LOCUS       DAVKFH010000001        86350 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKFH010000001 DAVKFH010000000
VERSION     DAVKFH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 86350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.001"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(388..480)
                     /locus_tag="VW006_000001"
     CDS             complement(388..480)
                     /locus_tag="VW006_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9378937.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            641..1612
                     /gene="ruvB"
                     /locus_tag="VW006_000002"
     CDS             641..1612
                     /gene="ruvB"
                     /locus_tag="VW006_000002"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9378938.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1593..2165
                     /locus_tag="VW006_000003"
     CDS             1593..2165
                     /locus_tag="VW006_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9378939.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2396..3154
                     /locus_tag="VW006_000004"
     CDS             2396..3154
                     /locus_tag="VW006_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9378940.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3163..3966
                     /locus_tag="VW006_000005"
     CDS             3163..3966
                     /locus_tag="VW006_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9378941.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            3988..5247
                     /gene="rseP"
                     /locus_tag="VW006_000006"
     CDS             3988..5247
                     /gene="rseP"
                     /locus_tag="VW006_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9378942.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5260..7113
                     /locus_tag="VW006_000007"
     CDS             5260..7113
                     /locus_tag="VW006_000007"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9378943.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7213..8592
                     /gene="bglA"
                     /locus_tag="VW006_000008"
     CDS             7213..8592
                     /gene="bglA"
                     /locus_tag="VW006_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9378944.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            8784..10592
                     /gene="glmS"
                     /locus_tag="VW006_000009"
     CDS             8784..10592
                     /gene="glmS"
                     /locus_tag="VW006_000009"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9378945.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10711..11760
                     /locus_tag="VW006_000010"
     CDS             10711..11760
                     /locus_tag="VW006_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9378946.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(11860..15657)
                     /gene="spuA"
                     /locus_tag="VW006_000011"
     CDS             complement(11860..15657)
                     /gene="spuA"
                     /locus_tag="VW006_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9378947.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDTHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVKIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16074..16487
                     /gene="rpsL"
                     /locus_tag="VW006_000012"
     CDS             16074..16487
                     /gene="rpsL"
                     /locus_tag="VW006_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9378948.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16507..16977
                     /gene="rpsG"
                     /locus_tag="VW006_000013"
     CDS             16507..16977
                     /gene="rpsG"
                     /locus_tag="VW006_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9378949.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17401..19482
                     /gene="fusA"
                     /locus_tag="VW006_000014"
     CDS             17401..19482
                     /gene="fusA"
                     /locus_tag="VW006_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9378950.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19586..23977
                     /locus_tag="VW006_000015"
     CDS             19586..23977
                     /locus_tag="VW006_000015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9378951.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24079..24342
                     /locus_tag="VW006_000016"
     CDS             24079..24342
                     /locus_tag="VW006_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9378952.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24335..24613
                     /locus_tag="VW006_000017"
     CDS             24335..24613
                     /locus_tag="VW006_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9378953.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24634..24786
                     /locus_tag="VW006_000018"
     CDS             24634..24786
                     /locus_tag="VW006_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9378954.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            24808..26049
                     /locus_tag="VW006_000019"
     CDS             24808..26049
                     /locus_tag="VW006_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9378955.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26059..26289
                     /locus_tag="VW006_000020"
     CDS             26059..26289
                     /locus_tag="VW006_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9378956.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            26647..27369
                     /locus_tag="VW006_000021"
     CDS             26647..27369
                     /locus_tag="VW006_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9378957.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            27586..28920
                     /gene="pepC"
                     /locus_tag="VW006_000022"
     CDS             27586..28920
                     /gene="pepC"
                     /locus_tag="VW006_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9378958.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(28976..29887)
                     /locus_tag="VW006_000023"
     CDS             complement(28976..29887)
                     /locus_tag="VW006_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9378959.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(29911..30714)
                     /locus_tag="VW006_000024"
     CDS             complement(29911..30714)
                     /locus_tag="VW006_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9378960.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(30742..31731)
                     /gene="manX"
                     /locus_tag="VW006_000025"
     CDS             complement(30742..31731)
                     /gene="manX"
                     /locus_tag="VW006_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9378961.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(31964..32983)
                     /gene="adhP"
                     /locus_tag="VW006_000026"
     CDS             complement(31964..32983)
                     /gene="adhP"
                     /locus_tag="VW006_000026"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9378962.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWAVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(33335..34147)
                     /locus_tag="VW006_000027"
     CDS             complement(33335..34147)
                     /locus_tag="VW006_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9378963.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            34337..35755
                     /locus_tag="VW006_000028"
     CDS             34337..35755
                     /locus_tag="VW006_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9378964.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            35808..36515
                     /locus_tag="VW006_000029"
     CDS             35808..36515
                     /locus_tag="VW006_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9378965.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            36610..37584
                     /gene="folP"
                     /locus_tag="VW006_000030"
     CDS             36610..37584
                     /gene="folP"
                     /locus_tag="VW006_000030"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9378966.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALATGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            37586..38908
                     /locus_tag="VW006_000031"
     CDS             37586..38908
                     /locus_tag="VW006_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9378967.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            38889..39443
                     /gene="folE"
                     /locus_tag="VW006_000032"
     CDS             38889..39443
                     /gene="folE"
                     /locus_tag="VW006_000032"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9378968.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            39486..40298
                     /gene="folK"
                     /locus_tag="VW006_000033"
     CDS             39486..40298
                     /gene="folK"
                     /locus_tag="VW006_000033"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9378969.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(40343..40660)
                     /locus_tag="VW006_000034"
     CDS             complement(40343..40660)
                     /locus_tag="VW006_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9378970.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            41017..41463
                     /gene="rplM"
                     /locus_tag="VW006_000035"
     CDS             41017..41463
                     /gene="rplM"
                     /locus_tag="VW006_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9378971.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            41482..41874
                     /gene="rpsI"
                     /locus_tag="VW006_000036"
     CDS             41482..41874
                     /gene="rpsI"
                     /locus_tag="VW006_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9378972.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(42136..42558)
                     /locus_tag="VW006_000037"
     CDS             complement(42136..42558)
                     /locus_tag="VW006_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9378973.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            42705..42881
                     /locus_tag="VW006_000038"
     CDS             42705..42881
                     /locus_tag="VW006_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9378974.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            43283..45496
                     /locus_tag="VW006_000039"
     CDS             43283..45496
                     /locus_tag="VW006_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9378975.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(45718..46194)
                     /locus_tag="VW006_000040"
     CDS             complement(45718..46194)
                     /locus_tag="VW006_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9378976.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQAQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFSKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            46417..49620
                     /locus_tag="VW006_000041"
     CDS             46417..49620
                     /locus_tag="VW006_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9378977.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKGG
                     AITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYYWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            50000..50385
                     /gene="tnpA"
                     /locus_tag="VW006_000042"
                     /pseudo
     CDS             50000..50385
                     /gene="tnpA"
                     /locus_tag="VW006_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(50764..51393)
                     /locus_tag="VW006_000043"
     CDS             complement(50764..51393)
                     /locus_tag="VW006_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9378978.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(51403..52404)
                     /locus_tag="VW006_000044"
     CDS             complement(51403..52404)
                     /locus_tag="VW006_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9378979.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLSSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(52435..53076)
                     /locus_tag="VW006_000045"
     CDS             complement(52435..53076)
                     /locus_tag="VW006_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9378980.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(53095..53910)
                     /locus_tag="VW006_000046"
     CDS             complement(53095..53910)
                     /locus_tag="VW006_000046"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9378981.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            54181..54615
                     /locus_tag="VW006_000047"
     CDS             54181..54615
                     /locus_tag="VW006_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9378982.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQESVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            54627..55817
                     /locus_tag="VW006_000048"
     CDS             54627..55817
                     /locus_tag="VW006_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEU9378983.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            55828..56319
                     /locus_tag="VW006_000049"
     CDS             55828..56319
                     /locus_tag="VW006_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9378984.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            56334..57113
                     /locus_tag="VW006_000050"
     CDS             56334..57113
                     /locus_tag="VW006_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9378985.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            57100..57918
                     /locus_tag="VW006_000051"
     CDS             57100..57918
                     /locus_tag="VW006_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9378986.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGENQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGKLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            57918..58211
                     /locus_tag="VW006_000052"
     CDS             57918..58211
                     /locus_tag="VW006_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9378987.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            58233..60134
                     /locus_tag="VW006_000053"
     CDS             58233..60134
                     /locus_tag="VW006_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9378988.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFMSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            60194..61195
                     /locus_tag="VW006_000054"
     CDS             60194..61195
                     /locus_tag="VW006_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9378989.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(61305..61640)
                     /locus_tag="VW006_000055"
     CDS             complement(61305..61640)
                     /locus_tag="VW006_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9378990.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(61651..61893)
                     /locus_tag="VW006_000056"
     CDS             complement(61651..61893)
                     /locus_tag="VW006_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9378991.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(61909..62103)
                     /locus_tag="VW006_000057"
     CDS             complement(61909..62103)
                     /locus_tag="VW006_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9378992.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            62269..63219
                     /gene="rsmH"
                     /locus_tag="VW006_000058"
     CDS             62269..63219
                     /gene="rsmH"
                     /locus_tag="VW006_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9378993.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            63231..63548
                     /gene="ftsL"
                     /locus_tag="VW006_000059"
     CDS             63231..63548
                     /gene="ftsL"
                     /locus_tag="VW006_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9378994.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            63552..65804
                     /gene="pbpX"
                     /locus_tag="VW006_000060"
     CDS             63552..65804
                     /gene="pbpX"
                     /locus_tag="VW006_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9378995.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            65806..66786
                     /locus_tag="VW006_000061"
     CDS             65806..66786
                     /locus_tag="VW006_000061"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9378996.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            67350..69455
                     /locus_tag="VW006_000062"
     CDS             67350..69455
                     /locus_tag="VW006_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9378997.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(69751..70233)
                     /locus_tag="VW006_000063"
     CDS             complement(69751..70233)
                     /locus_tag="VW006_000063"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9378998.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(70328..71812)
                     /locus_tag="VW006_000064"
     CDS             complement(70328..71812)
                     /locus_tag="VW006_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9378999.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDATRAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            71965..73575
                     /locus_tag="VW006_000065"
     CDS             71965..73575
                     /locus_tag="VW006_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9379000.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(73706..75136)
                     /locus_tag="VW006_000066"
                     /pseudo
     CDS             complement(73706..75136)
                     /locus_tag="VW006_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398391.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            75293..76739
                     /gene="cps4A"
                     /locus_tag="VW006_000067"
                     /pseudo
     CDS             75293..76739
                     /gene="cps4A"
                     /locus_tag="VW006_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
     gene            76741..77472
                     /locus_tag="VW006_000068"
     CDS             76741..77472
                     /locus_tag="VW006_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEU9379001.1"
                     /translation="MIDIHSHIVFDVDDGPKSIEESKALLREAYNQGVRMIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYREIHAGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVLKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKEFFVDNPRKIIMDQLI"
     gene            77481..78173
                     /gene="cpsC"
                     /locus_tag="VW006_000069"
     CDS             77481..78173
                     /gene="cpsC"
                     /locus_tag="VW006_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9379002.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            78183..78866
                     /gene="cpsD"
                     /locus_tag="VW006_000070"
     CDS             78183..78866
                     /gene="cpsD"
                     /locus_tag="VW006_000070"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEU9379003.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARTGYKTLLIDGDTRNSVISGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVIQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTAIGEVNKRDVQKAKQQLEQTEKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
     gene            78882..80249
                     /locus_tag="VW006_000071"
     CDS             78882..80249
                     /locus_tag="VW006_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9379004.1"
                     /translation="MDEKGLKIFLAVIQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMG
                     IDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLIL
                     FAIASLVLVPLIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTM
                     QGGMFKIDNDPRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            80255..80998
                     /locus_tag="VW006_000072"
     CDS             80255..80998
                     /locus_tag="VW006_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU9379005.1"
                     /translation="MNERMQILGVTIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCHEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEILQDMLKVFKQRYPNLNVVGYRNGYFSPEDEKKIQEDIKGGKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQKLNLEWLFRVVN
                     EPKRLFKRYFIGNVTFIKRVLDEKRKSKK"
     gene            80973..82058
                     /locus_tag="VW006_000073"
     CDS             80973..82058
                     /locus_tag="VW006_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9379006.1"
                     /translation="MKSENQKNNILHISRTMDIGGAERIVYQLSSDLKDEFDSVHVAS
                     TGGLWESKLLSKGVQHHKILDVDSKNPLTMVKILSSFYRIIKNNDITIVHTHHRMAAF
                     YIRLLKIINPKLIHVYTAHNVFKNKLPLYKFSLKKAYCVAVSKAVNDNLKHDVGISDS
                     TVIYNGVVFEKTDKLVGEIVNYNGIKLGCIARLSEQKGLTYLIDAMSLVKHRNITLFI
                     VGDGELRDELENKVKQLNLQKSVIFLGYRKDIVECINSFDYLVSSSLYEGFGLNAIEA
                     FMKGKTMVSSDIPGINEVVNNKNGILVPVKDDVALARAIEKLATDKKLREKLAYQAKK
                     DYETKFNYSLFLDNYRRLYRKLMGESK"
     gene            82055..83143
                     /gene="wecB"
                     /locus_tag="VW006_000074"
     CDS             82055..83143
                     /gene="wecB"
                     /locus_tag="VW006_000074"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU9379007.1"
                     /translation="MKKVMLVFGTRPEAIKMCPLVNELKQNDSIKTLVCVTGQHKEML
                     EQVLEVFKVVPDYDLGIMKANQTLFTITTSILDKIQAVLEKEKPDLVLVHGDTTTTFA
                     TALAAFYMGIKVGHVEAGLRTYNLQSPFPEEFNRQTTSIIADYHFAPTEVAKENLLRE
                     DRKNIFVTGNTVIDALKTTVQDNYHHPILEWAKGSKLIMLTAHRRENLGKPMENMFNA
                     VNRILNEFEDVKVVYPIHKNPKVRELASKVFGDNERMKIIEPLEVIDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVVAGTLKLVGTEEENIYSNFKLLLEDET
                     EYNKMSKASNPYGDGMTCKRIVDIILEV"
     gene            83185..84324
                     /locus_tag="VW006_000075"
     CDS             83185..84324
                     /locus_tag="VW006_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9379008.1"
                     /translation="MIKRKIYIICVLLSYFLPSFFTVGSMATLFALLSTLVKLFVTFD
                     YIPKVLLQHKVTLIDILVVLFLFFQAFAAFQSQTLYLNYVGGQFFLLGLYSFLKHFLL
                     LDCKTTIKSLFLTFILFLCVQVITQLLFPVGFDSLHPTGDNRLYFLGRKNIATPYIIV
                     GLGGFYLLNKKMNEFISLKEIIFLGLFGILSFLTQSSTAIICYGLFIFIRLLGLKENI
                     GKLYSLVSMAVYVCFSLSIIFSQSTILSTFTAIFSKNATFSGRINIWQLAIRIFEENF
                     WFGRGLNVNFNAWTNGIIVNSAHNTLLDILARTGIFPGVLFIVLLMNLFLGKYRIESK
                     TLLAMLASFMIYITMETSSMSILLLIIVLCVYWPRGEEKLHEQVT"
     gene            84308..85177
                     /locus_tag="VW006_000076"
     CDS             84308..85177
                     /locus_tag="VW006_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU9379009.1"
                     /translation="MSKLLNTIYYRLFRLYKKIYLQVFSKKTVDILGEKANLENELIV
                     SFTTIPSRLNYLPSMIKSIFNQTIIPNRFIMYVYKDEFEGINLESILELEIKNGLEIV
                     YVDENLRSHKKYFYAMKDNPNSIVVLVDDDIIYPRNTIKKLIASYRIYPQCVSAMRCH
                     RIKLFSDGSLYPYNQWEYEISGATIPSYFNFFTSGGGTLFPPCTRNEDLFNKKNIREL
                     SFLADDVWLNFLVVKNGIKTVKATRYKGTPLTIDDNPEESLVYLNTVYDNNNDKCIRN
                     MVEYYQINFTEDK"
     gene            85177..>86350
                     /locus_tag="VW006_000077"
     CDS             85177..>86350
                     /locus_tag="VW006_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEU9379010.1"
                     /translation="MNVEKNLKRGIFYTAIGKYSNVVIQLLVTAILSRILSPVEYGIV
                     AVVNVFLLFFQMLADSGIGPAIVQNKELNKSDLISIFSLTIYSGIVLSCIFALLGYPM
                     GVLYGETIYVSLFPLLGLCVLFYTITIVPQAILMKTMNFKIVNFLTIFSNIASGLVGV
                     ILAVSHFGVYSLIFSNIVKAMVLFIALYSKAKINFYWKVSKSSIGKIFEFSKFQFLFN
                     FWNYFARNLDNLLIGRLISPSDLGYYDKAYQLSLYPNQILSQVVTPALHPIMSNFQDN
                     ISKIGEVYLQISRIFVIVGIPISAYLYFNAQYVVTFMFGDNWSQSVPVFQILAATIWL
                     QMANSPTGAFYQATNQTKLLFRIGLLTSFINILAIVIGVMLQSIQCVAYMLLISFSL"
BASE COUNT        25702 a        15517 c        19318 g        25813 t
ORIGIN      
        1 gggacttttt tagcgaatat aactaagaaa taggtctgtg gtcgcttcag ctagttcaaa
       61 ctcaatttga ttgtaagtga ctgttaattg aagaactgga actactgtaa taatgatttt
      121 gcttgtctag tagtggcgta ttaaatgaga agtaacatgt aagattgtct cttcgtttac
      181 gaaatcaaaa tttctatgaa attgatagaa tccctaacgt tctcttaatc ttaaccgtct
      241 tgtcgtacag cggttgaaga aaatgtaaac ctttgacaaa attcgtgaac tatgggataa
      301 taaaaaggaa ttgagtacta gttctatatc ataggctaga aaagacccca agctcacgac
      361 caaataagct tgaggtcttt tttgacacta tttgtccttg tttagccatt tatcgactat
      421 acgaagaacg acaccgacca caattggtcc gatgatagtt ttgagtacta attccatcat
      481 gggctatctc acctcctttc gtaggcggtg tagcagtgtc gtagaatatt ataccacata
      541 agtgctaaac ttaggagtca cccaatcagg tggctttttt gtttgtggct tggttttttg
      601 atataataga gccatgagta gaattttaga taatgagata atgggggatg aggagttagt
      661 agaacgcacg ctccgtcctc agtatttacg tgaatatatc ggacaggata aggtcaagga
      721 ccagctacaa atctttattg aagctgccaa aatgcgggat gaagcgctgg atcatgtgct
      781 cttatttggg cctccaggtc tcgggaaaac gaccatggcc tttgttattg ccaacgaact
      841 gggagtcaat cttaagcaga cgtcgggtcc agtcattgaa aaagccggag atctggtagc
      901 tattttgaat gagttagagc ctggggatgt actttttatt gatgagatcc atcgtttgcc
      961 aatgtcagtg gaagaggtgc tttatagtgc tatggaggac ttctacatcg atattatgat
     1021 tggggctggt gagggtagtc gtagtgttca tttggagtta ccacctttta ccttgattgg
     1081 tgcgacgact cgggctggta tgctctccaa tccgctacgg gcacgttttg ggattacagg
     1141 ccatatggag tattatgccc atgctggctt gacagaaatt gtcgagcgga cggcagatat
     1201 ttttgagatg gaaatcactc atgaggcagc atctgagttg gccctacgta gtcgtgggac
     1261 ccctcgtatt gccaatcgtc tcctcaagcg cgtgcgcgat tttgcccaga taatggggaa
     1321 tggggtaatt gatgatatta ttaccgataa ggctttgact atgctggatg ttgaccatga
     1381 aggtttggac tatgtggatc aaaaaatcct tcgtaccatg attgagatgt acagtggagg
     1441 acctgttggt ctaggaactc tttctgtgaa tatcgccgaa gagcgtgaga cagttgaaga
     1501 tatgtatgag ccttacttga ttcaaaaagg ttttatcatg cggacacggt ctggacgggt
     1561 ggcgactgct aaggcatatg agcacttagg ttatgaatac agtgaaaaat aagcaagaaa
     1621 tcttagaggc ttttagagaa aatccagata tgatggccat tctgacgatc atccgagacc
     1681 ttggtctgaa agactcgtgg ttggcagcag gttctgtcag aaatttcatt tggaatcttt
     1741 tgtcagacaa atcccctttt gatcatgaaa cagatataga tgtgattttc tttgatccag
     1801 atttttctta tgaggaaacc ttattactgg agaaaaagct gcgagaggat tttcctcagt
     1861 accagtggga attgaaaaat caggtctata tgcatcagca cagtcctcac actgcttcct
     1921 ataccagttc tcgtgatgct atgagtaagt atccagaacg gtgtacgaca gttggactgc
     1981 gcttgaatga agaatcagat tttgaactct atgcacctta tggtttggag gatattttga
     2041 attttcaagt tcgtccaact cctcatttct tagaaaatga agaccgaatg gaactctatc
     2101 aaacacgttt atccaagaaa aattggcagg agaaatggaa aaatttgatt tttaaaaata
     2161 cttaaggaaa ctttaagcta gggagtgtac actaagttca taagttaaga agaccttaac
     2221 ttaaactcct aaaacttttt cataataatc tccctataaa aataaagtcg cctaatcagg
     2281 cggcttattt ttttgaaaaa tgggcttggt gcctgagaat aaatagctta gtgatagaag
     2341 aaaatgggga aatatggtat aatgaaacga tagatttttg aataggaata agatcatgtt
     2401 tggatttttt aagaaagata aggctgtgga agtagaggtt ccgacacagg ttcctgctca
     2461 tatcggcatc atcatggatg gcaatggccg ttgggctaaa aaacgtatgc aaccgcgagt
     2521 ttttggacac aaggcgggca tggaagcatt gcaaaccgtg accaaggcag ccaacaaact
     2581 gggcgtcaag gttattacgg tctatgcttt ttctacggaa aactggaccc gtccagatca
     2641 ggaagtcaag tttatcatga acttgccagt agagttttat gataattatg tcccggaact
     2701 acatgcgaat aatgttaaga ttcaaatgat tggggagaca gaccgcctgc ctaagcaaac
     2761 ctttgaagct ttaaccaagg ctgaggaatt gactaagaac aacacaggat tgattcttaa
     2821 ttttgctctt aactatggtg gacgtgctga gattacacag gcgcttaagt tgatttccca
     2881 ggatgtttta gatgccaaaa tcaacccagg tgacatcaca gaggaattga ttggtaacta
     2941 tctctttact cagcatttgc ctaaggactt acgagaccca gacttgatta tccgtactag
     3001 tggagaatta cgtttgagca atttccttcc atggcaggga gcctatagtg agctttattt
     3061 tacggacacc ttatggcctg attttgacga agcagccttg caggaagcta ttcttgccta
     3121 taatcgtcgc catcgccgat ttggaggagt ttaggaggaa atatgaccca ggatttacag
     3181 aaaagaacct tgtttgcagg gattgccctg gctattttcc taccaatttt aatgattggg
     3241 ggcctcttgc ttcagatagc aatcggaatc atagccatgc tagccatgca tgaacttttg
     3301 aagataagag gtctagcgac catgacgatg gagggcctct tgactctctt tgcaaccttt
     3361 gcattaacca ttcccttgga gaattacctg acttttttgc cagttgatgg gaatgtggtt
     3421 gcctatagtg ttttgatttc aatcatgtta ggaacgaccg tttttagcaa gtcttatacg
     3481 attgaggatg cggttttccc tcttgctatg agcttctacg tgggctttgg atttaatgct
     3541 ttactagatg ctcgtgttgc aggtttggac aaggctctct tagccttgtg tatcgtctgg
     3601 gcgacagaca gtggtgccta tcttgttggg atgaactatg ggaaacgaaa gttagcacca
     3661 agggtatcgc ctaataaaac ccttgagggt gccttgggtg gtattttagg agcaatttta
     3721 gtaaccatta tctttatgat agttgacagt acagttgctc ttccatatgg aatttacaag
     3781 atgtcagtct ttgctatttt ctttagcatt gctggacaat ttggtgattt actagaaagt
     3841 tcgatcaaac gtcattttgg tgttaaggat tctgggaaat ttatccctgg acatggtggt
     3901 gttttggatc gtttcgatag tatgttgctt gtatttccaa tcatgcactt atttggactc
     3961 ttttaatcaa aagacggagg aaacgctatg ctcggaattt taacctttat tctggttttc
     4021 gggattattg tagtggtgca cgagttcggg cacttctact ttgccaagaa atcagggatt
     4081 ttagtacgtg aatttgccat cggtatggga cctaaaattt ttgctcacat tggcaaggat
     4141 ggaacggcct ataccattcg aatcttgcct ctgggtggct atgtccgcat ggccggttgg
     4201 ggtgatgata caactgaaat caagacagga acgcctgtta gtttgacact tgctgatgat
     4261 ggtaaggtta aacgcatcaa tctctcaggt aaaaaattgg atcaaacagc cctccctatg
     4321 caggtgaccc agtttgattt tgaagacaag ctctttatca aaggattggt tctggaagaa
     4381 gaaaaaacat ttgcagtgga tcacgatgca acggttgtgg aagcagatgg tactgaggtt
     4441 cggattgcac ctttagatgt tcaatatcaa aatgcgacta tctggggcaa actgattacc
     4501 aattttgcag gtcctatgaa caattttatc ttaggtgtcg ttgttttttg ggttttaatc
     4561 tttatgcagg gtggtgtcag agatgttgat accaatcagt tccatatcat gccccaaggt
     4621 gccttggcca aggtaggagt accagaaacg gcacaaatta ccaagattgg ctcacatgag
     4681 gttagcaact gggaaagctt gatccaagct gtggaaacag aaaccaaaga taagacggca
     4741 ccgactttgg atgtgactat ttctgaaaag gggagtgaca aacaagtcac tgttacaccc
     4801 gaagatagtc aaggtcgtta ccttctaggt gttcaaccgg gggttaagtc agattttcta
     4861 tccatgtttg taggtggttt tacaactgct gctgactcag ctctccgaat tctctcagct
     4921 ctgaaaaatc tgattttcca accggatttg aacaagttgg gtggacctgt tgctatcttt
     4981 aaggcaagta gtgatgctgc taaaaatgga attgagaata tcttgtactt cttggcaatg
     5041 atttccatca atattgggat ttttaatctt attccgattc cagccttgga tggtggtaag
     5101 attgtgctca atatcctaga agccatccgc cacaaaccat tgaaacaaga aattgaaacc
     5161 tatgtcacct tggccggagt ggtcatcatg gttgtcttga tgattgctgt gacttggaat
     5221 gacattatgc gactcttttt tagataatcg aggaatatta tgaaacaaag taaaatgcct
     5281 atcccaacgc ttcgcgaaat gccaagcgat gctcaagtta tcagccatgc tcttatgttg
     5341 cgtgctggtt atgttcgcca agtttcagca ggtgtttatt cttatctacc acttgccaac
     5401 cgtgtgattg aaaaagctaa aaacatcatg cgccaagaat tcgaaaagat tggtgctgtt
     5461 gagatgttgg ctccaaccct tcttagtgca gaattgtggc gtgaatcagg tcgttacgaa
     5521 acctatggtg aagaccttta caaactaaaa aaccgtgaaa aatcagactt tatcttaggt
     5581 ccaactcacg aagaaacctt tacagctatt gtccgtgatt ctgttaaatc ttacaagcaa
     5641 ttgccactca acctttatca aattcagccc aagtatcgtg atgaaaaacg cccacgtaat
     5701 ggacttcttc gtacacgtga gtttatcatg aaggatgctt atagtttcca cgctaactat
     5761 gatagtttgg atagtgttta tgatgagtac aaagcagcct atgagcgtat tttcactcgt
     5821 agtggtttag acttcaaggc tattattggt gacggtggag ccatgggtgg taaggatagc
     5881 caagaattta tggccattac atctgctcgt acagaccttg accgctgggt tgtcttggac
     5941 aagtcagttg cctcatttga cgaaattcct gcagaagtgc aagaagaaat caaggcagaa
     6001 ttgctcaaat ggatagtctc tggtgaagat accattgctt actcaagtga gtctagctat
     6061 gcagctaact tagaaatggc aacaaacgag tacaaaccaa gcaaccgtgt tgtcgctgaa
     6121 gaagaagtta ctcgtgttgc aacgccagat gttaaatcaa ttgatgaagt tgcagccttc
     6181 ctcaatgttc cagaagaaca aacgattaaa accctcttct acattgcaga tggtgagctt
     6241 gttgcagccc ttctagttgg aaatgaccaa ctcaacgaag tcaagttgaa aaatcacttg
     6301 ggagcagatt tctttgacgt tgctagcgaa gaagaagtgg cgaatgttgt tcaagcagga
     6361 tttggttcac ttggaccagt tggtttgcca gagaatatta aaattattgc agatcgtaag
     6421 gtgcaagatg ttcgcaatgc agttgtcggt gctaacgaag atggctacca cttgactggt
     6481 gtgaacccag gccgtgattt tactgcagaa tatgtggata tccgtgaagt tcgtgagggt
     6541 gaaatttccc cagatggaca aggtgtcctt aactttgcgc gtggtattga gatcggtcat
     6601 attttcaaac tcggaactcg ctactcagca agcatgggag cagatgtctt ggatgaaaat
     6661 ggtcgtgctg tgccaatcat catgggatgt tacggtatcg gtgtcagccg tcttctttca
     6721 gcagtgatgg agcaacacgc tcgcctcttt gttaacaaaa cgccaaaagg tgaataccgt
     6781 tacgcttggg gaatcaattt ccctaaagaa ttggcaccat ttgatgtgca tttgattact
     6841 gtcaatgtca aggatgaaga agcgcaagcc ttgacagaaa aacttgaagc aagcttgatg
     6901 ggagctggtt atgaagtctt gacagatgac cgtaacgaac gtgtcggtgt taaatttagc
     6961 gatagtgact tgattggtct tccaatccgt atcactgttg gtaaaaaagc ggccgatggc
     7021 attgtagaag ttaagatcaa ggcaactggt gacaccattg aggttcatgc agataacgtg
     7081 cttgaaacgc ttgaaatcct aagcaagaaa taaaaactat aatcagaaga aaaacaagga
     7141 aaaaatgtaa ctagttttta ccttgttttt tctgtataat gggagaaatg agtagataaa
     7201 gaggtaaatg ttatgctaag atttccaaag gattttgtct ggggatcctc tacttctgga
     7261 ccgcagacag aaggacgtgt agctggtgac ggtaagggag acaatctctg ggattactgg
     7321 ttccaagtgg agccaaatcg ttactataat gggattggtt cagataagac atcgactttt
     7381 tatgaaaatt gggagcggga tattgagctt ttgttagaga ctggtcacac agtctttcgg
     7441 acttctattc agtggtcacg gatttttcca caaggctgtg gaaaagtcaa ccctcaaggt
     7501 gtggattttt atcgtaaggt ctttgaggct attaaggcta aaggaattcg tctgttagtc
     7561 aatctctatc attttgattt accttttgct cttcaagagg atggtgatgg ttgggaaaat
     7621 aaggcgacag tctcagccta tgaagactat gctcgttttt gttttgagac ttatggagat
     7681 ttagtggatc aatggattac ctttaacgag cccatcgttc ctgtagaatt tggttatttt
     7741 tatgatgccc attatccaca taaggtggat gcagaggcag ctgttaaggt agcctatcat
     7801 acacaattgg ccagcagccg ggctgttaag gcttgccatg aacttttgcc tgattccaag
     7861 attgggattg tcctcaactt gacaccggct tatccacgta gccagcatcc tgctgatgtc
     7921 aaggcagctc gtattgcggc cctttttcag gcccaatctt tcttagatcc atctgtcttg
     7981 gggacttatc cacaggagtt ggtagaaatc ttgcatgaac acggtctttt acctgatgct
     8041 acagaggaag agttggaact cattcgtgat aatacggtgg acttccttgg tgtgaactac
     8101 tatcaacctt tgcgtgttat ggctcctcga tttgctaagc atccagatag tccactctta
     8161 ccagaacatt tttacgagcc ttatgtgatg cctggacgta aaatcaattc tcaccgtggt
     8221 tgggagattt atgagcaagg gatttatgac atcgcccaaa atatcaagga aaattatggc
     8281 aatattgagt ggatgttgac tgagaatggt atgggtgttg aaggggaaga aaaattccgt
     8341 caagatggaa tgattcaaga tgattaccgt attgactttg taaaaggtca tcttcgtgaa
     8401 cttcaccgtg ccattgaaga tggtgccaac tgtaagggct acttgatttg gacctttatt
     8461 gactgctggt catggctcaa cagctataaa aatcgctatg gtttggtcga attagacttg
     8521 gaaacgcaag aacgtcgtct gaagaaatca ggccactggt tcaaagaatt aagcgataat
     8581 aatggatttt aaaaaggact ctgactgatt atcccaatcg gtcagagttt tttgcttaca
     8641 ggagcttaat ttgaactata ccaattttta ctcttgacaa gtgaaagaaa caagtatata
     8701 ctgtttttgc tgattctata aaagatgaat tagactatac caattttaag gagaaagcac
     8761 agcttgcctg tgtcgtatat actatgtgtg gaattgttgg tgttgttgga aacacaaatg
     8821 caactgatat tttgattcaa gggcttgaaa agcttgaata ccgtggctat gattctgcgg
     8881 gaatttttgt cctagatggt gctgataacc atttggtgaa ggctgttggt cgtatcgcag
     8941 aattgtctgc caagacagct ggtgttgagg gaacaactgg tatcggacat actcgttggg
     9001 caactcatgg gaaaccaacg gaagacaatg ctcacccaca ccgctctgag acagaacgtt
     9061 ttgtcttggt tcacaatggg gtgattgaaa actaccttga aattaaagaa gaataccttg
     9121 ctgggcacca cttcaaaggg caaacagata cggaaatcgc cgtacatttg attggaaaat
     9181 ttgcggaaga agacggtctc tcagttcttg aagcctttaa aaaagctctt catattatcc
     9241 gtggttcata tgcctttgcc ttgattgact ctgaaaatcc agatgtcatc tatgtagcga
     9301 aaaacaaatc tccacttttg attggtcttg gggaaggcta caatatggtc tgctcagatg
     9361 ctatggctat gattcgtgaa accaaccaat acatggaaat tcatgaccaa gagttggtaa
     9421 tcgtcaaggc tgatagcgtg gaagttcaag actatgatgg taacagtcgt gaacgtgcta
     9481 gctatactgc ggaacttgac ttgtcagata tcggtaaggg aacttatcct tactacatgc
     9541 ttaaggaaat tgatgagcaa ccaactgtta tgcgtaaact cattcaagcc tacacggatg
     9601 atgctggtca agtagtgatt gatcctgcta tcattaaggc tgttcaagac gcagaccgca
     9661 tctacatcct tgcagctgga acatcttacc atgcaggatt tgcttctaag aaaatgttgg
     9721 aagaattgac agatacacca gttgaacttg gcatctcatc tgagtggggc tacggtatgc
     9781 cacttctcag caagaaacca ctcttcatct ttatcagcca atctggtgaa acagcggata
     9841 gtcgtcaagt tttggtcaag gctaatgaaa tgggaattcc aagcttaaca gtgacaaacg
     9901 ttccaggttc aaccctctca cgtgaagcca actataccat gctccttcac gcaggtcctg
     9961 aaattgccgt ggcatcaact aaagcctata cagcgcaaat cgcagccctt gccttccttg
    10021 caaaagcagt cggagaagca aatggtaatg ctaaagcgca agcctttgac ctggttcatg
    10081 aattgtcaat cgtagctcag tctatcgaat caactctttc agagaaagaa accattgaag
    10141 ccaaggttcg tgaacttctt gaaacaactc gtaacgcctt ttacatcgga cgtggtcaag
    10201 attactacgt agccatggaa gcaagtctca aactcaaaga gatttcttat atccagtgtg
    10261 aaggttttgc ggcaggagaa ctcaagcacg gaaccattgc cttgattgaa gaaggaacac
    10321 ctgtcttggc tctcttgtca gatccagtcc ttgccaacca cactcgtgga aatatccaag
    10381 aggtggcagc ccgtggtgct aaggtcctca ctatcgcaga agaaaatgtt gctaaagata
    10441 ccgacgatat cgtccttacg actgtacacc cttacctctc accaatttca atggtcgtac
    10501 cgacgcaatt ggttgcttac tttgcaaccc tccaccgtgg cctcgatgtg gataaaccac
    10561 gtaaccttgc taaatcagta acggtagaat aagctaaaaa agtctagtta tctaggcttt
    10621 ttctaatgag caaattggtt gcttgtactc aagattcgtg ttagaataat ttttcaaatt
    10681 gaaggacaga gatagacaag gagaatcaca atggtagaat tgggaatttc aacatttggg
    10741 gaaataacgg agcttgaagg gactggacaa acttacagtc atgctgaacg cattcgccag
    10801 ttggtggcag agattgagct ggctgacaag gttggtttgg acgtatatgg gattggcgag
    10861 caccatcggg cggattttgc agtatcagcc ccagagattg tcctggccgc tggggcagtc
    10921 aataccaaga aaatccgttt gaccagtgca gtcagcattc tgtcaagcat ggatccgatt
    10981 cgtttgttcc aacagtatgc cactatcgat gctttgtcaa atggacgatc ggagattatg
    11041 gctggaaggg gctctttcac ggaatctttc cctctgtttg gttatgactt gaaagactac
    11101 gactctcttt ttgatgagaa attagacttg cttcagttag tcaatgaaaa gaccaagtta
    11161 gactggcaag gtcgattgac tcaaacgatt gctggtaaag aagtttatcc tcgcccagtt
    11221 caggacaaat tgcccttgtg gatagctaca ggtggtcatg tcgaatcaac agtgaagatt
    11281 gctcaggctg gtctaccgat tgtatatgct attattggtg gtaatccacg ttattttaaa
    11341 aagttgattc aggcttatcg tgagattgga agcgaagcgg gccatgctga caaagacttg
    11401 aaagtgggag cccattcttg ggggtggatt gcggaagatg gtgagcaggc ggtgaaagat
    11461 tatttccatc cgactaagca agtggtggat gctatttcca aagaccgtcc gcactggcag
    11521 gaattgcgtt atgagcaata tttggagcag gtagggccaa atggcgccat gtttgttggc
    11581 aatccagatc aggtggccga aaaattgatt cgcatgatag aggatttagg attggaccgc
    11641 ttcatgcttc atctaccgct tggttctatg cctcatgacc aggttctgag agctattgaa
    11701 ctcttcggaa cacaagttgc acccaaagta cgagcttatt ttgctatgaa agaggcttaa
    11761 taaaaaatcc taatcaagtt tattaggaca acaaatattg tacaaaaatt aatcccccga
    11821 gctataatgc ttgggggatt tttctatagg agggctagtt tagttctctt ttttattttt
    11881 tagtaagaaa ccgagaccaa ggagcgcaag gaggctgatt cctgcaaata ggagtttgtt
    11941 ttcgtttttg attcctgtat ttggaagttc agcttgttta tctggggttg caggtatatt
    12001 ttccttgcta gagttttcta ccgattcgtt tcgaaccgct tcttttacag atgcttcttg
    12061 tgcttcttgt gctggttgtt cagcttgtga atcagctgta gcttggctag gtttattttc
    12121 cgcatcagct actttggcat ctggtttagt agaatcaggt ctagcttctg gagctgggtc
    12181 ttgatgtgca gggtgagaag cttcattttg atgttcaggc ttggaagggg ttgagtctgg
    12241 tttctcttct gcagttgact catggctagt tccattttga gagactcgaa gaacagtagc
    12301 tgtaagggca ttcaatttca agcctttttc agtccattca agtcctttcg ggttggcaat
    12361 tttgactgat cctgcttggt tttcatctgc caaaacttcc gcatttctta gatgtgcaaa
    12421 ggcagttccc aaattaaatt cgcgagcttt ttcatccgca ttgacaaaga ctgcgtagat
    12481 atcgccgttt ggagcagtga tttggtagcc aatcactaca tcctcttttt ccacaccatt
    12541 ttggcctggg acagtgatga ggtggacacg gtctttgata tcttggagac tcttaagtcg
    12601 gaaggcatct gtagattgac gaagggcaat caaacctttc atatagtcac ggctcttgac
    12661 attttcagga taagctttac catctgtagc cttagtccag tcaaacttgt tgactgcgtc
    12721 gctagaatcg taagaatcat ggatgaagta aggatagtca aatgggttgc cgtccttatc
    12781 acgcaacaag tgagatttgt ttggaacctt atcctctgct actggagtct tgtaggctgg
    12841 gtcacggaat tgtttagtac gtccatattc ctgaccggag tggataaatg gagttccttg
    12901 agctgtcaag accatgagat ttccaagtcg taaacgacgg tggatttcag catagttctc
    12961 agccttgctt gggtcttttt tgatagactg ggcaatgatg tcaaagaggg tcaagttatc
    13021 atgggctgct atgtattgga tgacatctcc agggctgtca gcttcaaagt tagttggttg
    13081 agcaatgaga tttttaaaga tggtgttgac atcacgcttg ccacctgtga taaaggcagg
    13141 ttgaccttcg tttggataac cagatttgag gttgttacgg atgtcatctg aaaagacagc
    13201 gacagtatcg gtatgtttca tccaatcttg gtcagcagct ttagtaggca tgttttcatc
    13261 accggcatag gttctccaac cttcaccaag catgatgagg tttggattga gggcgcgtgc
    13321 agccttgtaa gcttcttcga tagaagcggc gtcatggtct cccatcatat cgaaacggaa
    13381 tccatccact ttgtaggtat caactaggta tttgatagag tcaactagga gccgtttggt
    13441 catatggtgg gttgttccca agcgtccacc accaaagcta gttcgaggtg tgccatcggc
    13501 atccataaag tggtagtagt ttggctctag gtcttcaaag atatcgactt tggctgtgtg
    13561 gttataaacg acatctagga tagctcccat accacgtttg tggatttcgt tgatgaggtt
    13621 tttaaattct gcgattcgtt tttctggatt cttaggatcg cttgagtaca taccagtcaa
    13681 ggagaagtag ttttgagggt catatcccca gttgtagttg ctgttgcttg aagcgtagtc
    13741 agacaagcgt tcatggtttt tcaattcatt gacaaagtag taagacaaga ctggaaggag
    13801 ctggatatgg gttacaccca agtctttgag atagtctagt ttttcaatga aggcttcaaa
    13861 agtcccaaat ggtttggtca agtcttttgc aatggcagga tctgaagtga agtcacgcac
    13921 atgagcttcg tagataacgg cgtcttcacg agtcttgaaa ttgtgaatct taccataagt
    13981 caagtcttga ggtccgagtt tagctggatc tacaaaggcg gctttagcca ctttatgggt
    14041 atcgtcaatt ttggcatcgt cgctattcca agcagcaaga gatttagcgt aaggatcgag
    14101 tgcaagaaca gttttacctt gacgctcgat ttggtattga taatagtagc cagtgaaatc
    14161 tgtgattccg agtttgtttg tgctgtctag agtttgtttc caagttcctc tttccccttt
    14221 ttcgagagcg acagttccaa ctactttgtc agggtcattc ttgtcgtaga caacaacaga
    14281 aaccttatca gcacttggtg accaaagggt caaatcaact tgttttcctt cttcttttag
    14341 gtcagctccc agtttgccat catagctgta tgtctcatct ttcaggtgcc agcttgtttt
    14401 ggtagtgaat tggtcggaat tgtagctaac agtataaggg tgttttgtgt cagagaaatc
    14461 tccgctgtag gtcactttct taccagcttc atcgattgca acatcggtaa tagttacctt
    14521 gtttcctagg tgattagtga tgttggagtg tttgaggata tcttcttttt tagcaccgac
    14581 aagtgttgaa aagctacttt caatgctaga agtgcctacg tgttgggctc ctgtcatacg
    14641 gatatcatgg acatagtatg gatttgtgta aatcgattca tcatcgtctt ttaggaaaat
    14701 ttggctatga tttttcaaat ctgtgaactt ataattttct ttacggattt tcacgtcgtc
    14761 tccttgtttg ctctcatcta gtaataaaaa tccaaattct cttgcggctt cattaagagg
    14821 aatgtcgata tagcggccat atttgcctgt agccgtaaag tctgttccgt caggccattg
    14881 agcgctactt ggatttttca catctcccca gtaccagaga gatttcttgt catagttgcc
    14941 atctgtgcgg tagtagttga cgcgaacagt tcctgcaggc tgtggctcgt aagagaaaac
    15001 cttgtaatct tggtctaacc aagcttcgtt catttttgga actagttttt ctacagattt
    15061 atcgccggtt agattttttc cagctgtatt gttgatgagg aagctaattt tcttggcttg
    15121 ttctcccttt aatttgacat ctaggtaata gccgtagtca tctttcttgg catccttgaa
    15181 ggacaaagct ccgtttggcc agttttcaga tggtttttca acatcgtccc aagtccatag
    15241 tccttgagca tccttgtttt cttcaggaag ttttttgaca tggatacgga agtagttatc
    15301 ttcgatgggc tcttctgcct tagtttcagt tgttactgga ctagtagaag tagttggctc
    15361 tgttgcaggt ttttcttctg ttttctctag ggaagcgttt gcatttctag gtgaagaaat
    15421 atcactttcg ttcttaccag agccagctgc tgtatcagta agaggttggg caagggtggt
    15481 tgtatttctg ttgttgggtt cgttagtggt tggtgtactt tcgtcggatg agatagttgg
    15541 tgtagccatg gcaagcaaga caaggcttgc gccgataagg acagaaccag ttccattttt
    15601 gagggaacgg atgctgtaga ccattttttt ctcagtgtga gatggtgttt ttctcataat
    15661 gattctccaa tcaataaatt tctatatcag tatagcatgt agtaaaacaa tatgcaagcg
    15721 ttttctttga tttgtgaaaa aaattcttac ttttgagaag ttttctgtac tattttatat
    15781 atcagtataa atgacggaga taaaaatgtc acataagcca agccgtatta ttaggaagat
    15841 gatgtgttcc cagctatttt tattcagaaa ttgatctcta acattaccaa taaaaaagaa
    15901 aaatatcttg acaagcaagg gaaaattttg ttacaataat agacggtact ttttactttt
    15961 ggtctctcaa gagtgtacag ggacgtgctg acaaatgttg caaaagtaca cacagatgat
    16021 atctgtcacc aagtgtatca tcaccaaaaa taaaaaaaca caggagaatg tagatgccta
    16081 caattaacca attggttcgc aaaccgcgta aatcaaaagt agaaaaatct aaatcaccag
    16141 ctttgaacgt tggttacaat agtcataaaa aagttcaaac aaacgtttct tcaccacaaa
    16201 aacgtggtgt tgcaactcgt gttggaacaa tgacacctaa aaaacctaac tcagcccttc
    16261 gtaaattcgc tcgtgtacgt ttgagcaacc ttatcgaagt tactgcctac atcccaggta
    16321 tcggacacaa cttgcaagag cacagcgtgg tgcttcttcg cggtggacgt gtaaaagacc
    16381 ttccaggggt acgttaccat atcgtccgtg gtgcacttga tactgcaggt gttaacgatc
    16441 gtaaacaagg ccgttctaaa tacggtacta aacgtccaaa agcataagga aaggggataa
    16501 agagaaatga gtcgtaaaaa tagagctcca aaacgtgacg tattgccaga tccgctatac
    16561 aattcacaac tagttactcg tcttatcaac cgcgttatgc ttgatggtaa acgtggtact
    16621 gctgcttcaa tcgtttacgg tgcttttgag caaatcaaag aagctactgg caacgatgca
    16681 cttgaagtat ttgaaacagc tatggaaaac atcatgcctg tacttgaagt acgtgcacgt
    16741 cgtgttggtg gttctaacta ccaagtccca gttgaagttc gtccagaacg tcgtacaaca
    16801 cttggacttc gttggttggt aacaatcgct cgtcttcgtg gtgaacacac aatgcaagac
    16861 cgtcttgcaa aagaaatctt ggatgctgct aacaacactg gtgcagcagt taagaaacgt
    16921 gaagatactc accgtatggc tgaagctaac cgtgcattcg cacacttccg ttggtaagat
    16981 aggatgcgaa agcgttaaga aagtcccaga gaaaataggg aatcgaagca ggttgcggtt
    17041 gcaaccaatg agattcatct ttttctccag acttttagct tgagctcaac taaatcatga
    17101 tgctaggcac ggaaggatgc aaggtgaaaa taggaaactg acgcagtatt caacgaatac
    17161 aaggagtttt atctttttca cgcagcatcc cgttccagct cacatcggct aactaacttt
    17221 agcctgggtt caaattagct aaatcgatta gtattagcta taactcagct taccatctcg
    17281 taagttgaaa ccaacaatag catgaaaaca ttgagaacgg gtaggtcctg cctatccgtt
    17341 tttattaaaa tcgtgttata atagaataga aatcaaaaat aaataggaga aacaaacctc
    17401 atggcacgcg aattttcact tgaaaaaact cgtaatatcg gtatcatggc tcacgtcgat
    17461 gccggtaaaa caacaactac tgagcgtatt ctttactaca ctggtaaaat ccacaaaatc
    17521 ggtgaaactc acgaaggtgc gtcacaaatg gactggatgg agcaagagca agaacgtggt
    17581 atcacgatca catctgctgc gacaacagct caatggaaca accaccgcgt aaacatcatc
    17641 gacacaccag gacacgtgga cttcacaatc gaagtacaac gttctcttcg tgtattggat
    17701 ggtgcggtta ccgttcttga ctcacaatca ggtgttgaac ctcaaactga aacagtttgg
    17761 cgtcaagcaa ctgagtacgg agttccacgt atcgtatttg ccaacaaaat ggacaaaatc
    17821 ggtgctgact tcctttactc tgtaagcaca cttcacgatc gtcttcaagc aaatgcacac
    17881 ccaatccaat tgccaatcgg ttctgaagat gacttccgtg gtatcattga cttgatcaag
    17941 atgaaagctg aaatctatac taacgacctt ggtacggata tccttgaaga agacatccca
    18001 gctgaatacc ttgaccaagc tcaagaatac cgtgaaaaat tgattgaagc agttgctgaa
    18061 actgacgaag aattgatgat gaaatacctc gaaggtgaag aaatcactaa cgaagaattg
    18121 aaagctggta tccgtaaagc gactatcaac gttgaattct tcccagtatt gtgtggttca
    18181 gccttcaaaa acaaaggtgt tcaattgatg cttgatgcgg ttatcgacta ccttccaagt
    18241 ccacttgaca tcccagcaat caaaggtatt aacccagata cagacgctga agaaactcgt
    18301 ccagcatctg acgaagagcc atttgcagct cttgccttca agatcatgac tgacccattc
    18361 gtaggtcgtt tgacattctt ccgtgtttac tcaggtgttc ttcaatcagg ttcatacgta
    18421 ttgaatactt ctaaaggtaa acgtgaacgt atcggacgta tccttcaaat gcacgctaac
    18481 agccgtcaag aaatcgacac tgtttactca ggtgatatcg ctgctgccgt tggtttgaaa
    18541 gatactacaa ctggtgactc attgacagat gaaaaagcta aaatcatcct tgagtcaatc
    18601 aacgttccag aaccagttat ccaattgatg gttgagccaa aatctaaagc tgaccaagac
    18661 aagatgggta tcgcccttca aaaattggct gaagaagatc caacattccg cgttgaaaca
    18721 aacgttgaaa ctggtgaaac agttatctca ggtatgggtg aacttcacct tgacgtcctt
    18781 gttgatcgta tgcgtcgtga gttcaaagtt gaagcgaacg taggtgctcc tcaagtatct
    18841 taccgtgaaa cattccgcgc ttctactcaa gcacgtggat tcttcaaacg tcagtctggt
    18901 ggtaaaggtc aattcggtga tgtgtggatt gaatttactc caaacgaaga aggtaaagga
    18961 ttcgaattcg aaaacgcaat cgtcggtggt gtggttcctc gtgaatttat cccagcggtt
    19021 gaaaaaggtt tggtagaatc tatggctaac ggtgttcttg caggttaccc aatggttgac
    19081 gttaaagcta agctttatga tggttcatat cacgatgtcg actcatctga aactgccttc
    19141 aagattgcgg cttcactttc ccttaaagaa gctgctaaat cagcacaacc agctatcctt
    19201 gaaccaatga tgcttgtaac aatcactgtt ccagaagaaa accttggtga tgttatgggt
    19261 cacgtaactg ctcgtcgtgg acgtgtagat ggtatggaag cacacggtaa cagccaaatc
    19321 gttcgtgctt acgttccact tgctgaaatg ttcggttacg caacagttct tcgttctgca
    19381 tctcaaggac gtggtacatt catgatggta tttgaccact acgaagatgt acctaagtca
    19441 gtacaagaag aaattattaa gaaaaataaa ggtgaagact aatccgtcct cactctagaa
    19501 ggaagtcact tagtggcttc cttttgtctt tagaaaatac ctctaaatat ggtaaaatag
    19561 tagaagaata atgtgaggaa aatgaatgtc aaatagtttt gaaattttga tgaatcaatt
    19621 ggggatgcct gctgaaatga gacaggctcc tgctttagca caggccaata ttgagcgagt
    19681 tgtggttcat aaaattagta aggtatggga gtttcatttc gtattttcta atattttacc
    19741 gattgaaatc tttttagaat taaagaaagg tttgagcgaa gaattttcta agacaggcaa
    19801 taaagctgtt tttgaaatta aggctcggtc tcaagaattt tcaaatcagc tcttgcagtc
    19861 ctactatagg gaggctttct ctgaaggtcc atgtgctagt caaggtttta agtcccttta
    19921 tcaaaatttg caagttcgtg ctgagggtaa tcagctattt attgaaggat ctgaagcgat
    19981 tgataaggaa cattttaaga agaatcatct tcctaattta gccaaacaac ttgaaaagtt
    20041 tggttttcca acttttaact gtcaagttga gaagaatgat gtcctgaccc aagagcagga
    20101 agaggccttt catgctgaaa atgagcagat tgttcaagct gccaatgagg aagcgctccg
    20161 tgctatggaa caactggagc agatggcacc tcctccagcg gaagagaaac cagtctttga
    20221 ttttcaagcg aaaaaagctg cagctaaacc caagctggat aaggcggaga ttactcctat
    20281 gatcgaagtg acgacagagg aaaatcgtct ggtatttgaa ggggttgttt ttgatgtgga
    20341 gcaaaaagtg actagaacag gtcgtgtttt aatcaacttt aaaatgacgg actatacttc
    20401 aagtttttct atgcaaaagt gggttaaaaa cgaggaagag gcccagaagt ttgacctcat
    20461 caagaagaat tcttggctcc gagttcgagg gaatgtggag atgaataact tcacacgcga
    20521 tttgactatg aacgtacagg atctgcagga agttgttcac tatgagcgga aggatttgat
    20581 gccagaaggt gagcgtcggg ttgagtttca tgctcatact aacatgtcga ctatggatgc
    20641 tttgccagag gtcgaagaga ttgttgcaac agctgctaag tggggacaca aggcggttgc
    20701 tatcacagac catgggaatg tccagtcctt tccacatggc tataaggcgg ctaagaaagc
    20761 gggaatccag ctgatctatg ggatagaagc caatatcgtg gaggaccgtg tccctatcgt
    20821 ctataacgaa gtggagatgg acttatcaga agcaacctac gtggtctttg acgtggaaac
    20881 gacgggactt tcagctatct ataatgactt gattcaggtt gcggcctcta agatgtacaa
    20941 ggggaatgtt attgctgaat ttgatgaatt tatcaatcct gggcatccct tgtcagcttt
    21001 tactacagag ttaactggaa ttacagatga tcatgtcaaa aatgccaaac cactagaaca
    21061 agttttgcaa gaattccaag aattttgcaa ggatacggtc ctagttgccc acaatgctgc
    21121 ctttgacgtt ggctttatga atgctaatta tgagcgtcat ggtcttccaa agattagtca
    21181 gccagttatt gatacgctgg agtttgctag aaacctctat cctgagtata aacgccatgg
    21241 tttggggcct ttgaccaagc gttttggtgt ggccttggaa catcaccaca tggccaacta
    21301 cgatgcggaa gcgactggtc gtctgctttt catctttatc aaagaggtag cagaaaaaca
    21361 tggtgtgacc gatttagcta gactcaacat tgatctaatc agtccagatt cttacaaaaa
    21421 agctcggatc aagcatgcga ccatctatgt caagaatcag gtaggtctaa aaaatatctt
    21481 taagctggtt tccttgtcta ataccaagta ttttgaagga gtgccacgga ttccgagaac
    21541 ggttctagat gcccatcgag agggcttgat tttaggttca gcctgttcag agggtgaagt
    21601 ttttgacgtg gtcgtttctc aaggtgtgga tgcggcggtt gaggtggcca agtattatga
    21661 ctttatcgag gtcatgccac cggctatcta tgcgcccttg attgctaaag agcaggtcaa
    21721 ggatatggag gaactccaga ccattatcaa gagtttgata gaggttggag accgccttgg
    21781 caagcctgtt ttggctacgg gaaatgttca ctatatcgaa ccggaagaag agatttatcg
    21841 tgaaattatc gtccgtagtt tgggacaggg tgcgatgatt aaccgaacta tcggtcatgg
    21901 tgaacatgcc caaccagcac cacttccaaa ggctcatttt cgaacgacta atgagatgtt
    21961 ggatgaattt gcctttttgg gagaggaact ggctcgtaaa ctggttattg aaaacaccaa
    22021 tgccttggca gaaatatttg aacccgttga agtcgttaag ggtgacttgt atacgccttt
    22081 catcgacaag gctgaagaaa cagttgctga gttgacctat aagaaagctt ttgagattta
    22141 tggaaatccg ctgccagata ttgttgattt gcggattgaa aaagaattaa catccatact
    22201 ggggaatgga tttgctgtga tttatctggc atcgcagatg ctggtgcaac gttctaatga
    22261 acggggttat ttggttggtt ctcgtgggtc tgtcggatct agtttcgttg cgaccatgat
    22321 tggaattacg gaggtcaatc ctctctctcc tcactatgtc tgtggtcagt gtcagtacag
    22381 tgagtttatc acagatggtt cgtacggttc aggatttgat atgccccata aggactgtcc
    22441 aaactgtggt cacaaactca gtaaaaacgg acaggatatt ccgtttgaga ccttccttgg
    22501 ttttgatggg gataaggttc ctgatattga cttgaacttc tcgggagaag atcagcctag
    22561 cgcccacttg gatgtgcgtg atatctttgg tgaagaatat gccttccgtg cgggaacagt
    22621 tggtacggta gctgccaaga ctgcctatgg atttgtcaag ggttacgagc gagattatgg
    22681 caagttttat cgtgatgcag aagtagaacg cctcgctcaa ggagcggcgg gtgtcaagcg
    22741 gacaacaggc caacacccgg ggggaatcgt tgttattccg aactacatgg atgtctacga
    22801 ttttacgcct gtccagtatc cagcagatga tgtcacggct gaatggcaga ccactcactt
    22861 taacttccac gatatcgatg agaacgtcct caaactcgat gtactgggac atgatgatcc
    22921 gaccatgatt cgaaaacttc aggatttgtc tggtattgac cctaataaaa ttcctatgga
    22981 tgacgaaggc gtgatggcac tcttttctgg gactgatgtg ctaggggtaa cacctgaaca
    23041 aattggaacg cctacgggta tgttggggat tccagagttt ggaacaaatt tcgtacgtgg
    23101 aatggtagac gaaacccatc cgacaacctt tgcggaattg cttcagctgt ctggtctgtc
    23161 ccacggtact gatgtttggt tggggaatgc tcaggatctg attaagcaag gaatagcgga
    23221 cctatcgact gttatcggtt gtcgggacga catcatggtt tacctcatgc atgcgggtct
    23281 ggaacctaag atggccttta ccattatgga acgggtacgt aagggtttgt ggctaaagat
    23341 ttcagaagag gagagaaatg gctatatcga agcaatgaag gctaataagg tgccagagtg
    23401 gtatatcgaa tcctgtggga aaattaagta catgttccct aaggcccatg cggcagccta
    23461 cgttatgatg gccttgcgtg tagcttactt caaggttcac catcctattt attactactg
    23521 tgcttacttc tccattcgtg ctaaggcttt tgatatcaag accatgggtg cgggcttgga
    23581 ggtcatcaag cgcagaatgg aagaaatctc tgaaaaacgg aagaacaatg aagcctctaa
    23641 tgtggaaatc gatctctata caactcttga gattgtcaat gagatgtggg aacgaggttt
    23701 caagtttggt aaattagatc tctaccgtag tcaggcgaca gagttcctca tcgacgggga
    23761 tacccttatc ccaccatttg tagcaatgga tggtctggga gagaacgttg ccaagcaact
    23821 ggtgcgggcg cgtgaagagg gagaattcct ctctaaaaca gaactacgca agcgtggtgg
    23881 actctcatca accttggttg aaaagatgga tgagatgggt attcttggaa atatgccaga
    23941 ggataaccag ttgagtttgt ttgatgagtt gttttaaaaa attgcttaat aatctattaa
    24001 aagaggctaa cgtatatcca atagatttac attagctttc ttttttgtta aaatagtcta
    24061 tggaaagagg gtgagagtat gtcaaagatg agtataagca tccgtctgga tagtgaggtt
    24121 aaggagcagg cccaacaggt gtttagtaat ctgggaatgg atatgacaac agctattaat
    24181 attttccttc gtcaggcaat tcaatatcag ggattacctt ttgatgttag actagacgaa
    24241 aatcggaagt tgctccaagc gttaacggat ttagaccaaa atcgtaatat gagccagtct
    24301 tttgaatcag tctcagattt gatggaggac ttacgtgctt aagattcgtt atcataaaca
    24361 gtttaaaaaa gattttaagt tggctatgaa gcgtggtttg aaggcagaat tattagaaga
    24421 agttttgaat tttctggttc aagaaaaaga acatcctgcc agatatcgtg atcattcatt
    24481 gacggcatcc aagcattttc aaggagttcg tgaatgccat acccagccag attggctttt
    24541 ggtttataaa gtagacaagt cggaattgat tttaaatttg ctgaggacag gcagtcacag
    24601 tgatttattt taatctattt taagggggtt ctcatgaaac taagaatatt tgcggaagat
    24661 aagccggcta agaaggtatt tgaatatcaa ttagaacttg ctgatcgtac aattcttcta
    24721 tcgacagcac tcttgtcagg tgctattgct ttagcaggaa tcttttctgc tttgaaagaa
    24781 aaataaaaat agaaaagaga aaacagaatg gttttaccaa attttaaaga aaatctagaa
    24841 aaatatgcga aattgttggt tgcgaacgga attaacgtgc aacctggtca cactttggct
    24901 ctctctattg atgtggagca acgtgaattg gcacatctaa tcgtgaaaga agcttatgcc
    24961 ttgggtgcgc atgaggtcat cgttcagtgg acagatgatg tgattaaccg tgagaaattc
    25021 ctccatgccc cgatggagcg tttggacaat gtgccagaat acaagattgc tgagatgaac
    25081 tatcttttgg agaacaaggc tagccgtctt ggggttcgtt catctgatcc aggtgccttg
    25141 aacggagtgg acgctgacaa gctttcagct tctgctaaag ctatgggact tgccatgaag
    25201 cctatgcgta tcgcaactca atctaacaag gttagctgga ctgtagcagc cgctgctgga
    25261 cttgagtggg ctaagaaagt cttcccaaat gctgcgagcg acgaagaagc agttgatttc
    25321 ctttgggatc aaatcttcaa aacttgtcgt gtctacgaag cagatcctgt taaggcttgg
    25381 gaggaacatg cagccattct caagagcaag gccgatatgc ttaataagga gcaattttca
    25441 gcccttcact acacagcgcc aggaacagat ttaacacttg gtttgccaaa gaaccacgtt
    25501 tgggaatcag ctggtgctgt caatgcacag ggcgaagaat tcttgccaaa tatgccgaca
    25561 gaagaggtct tcacagcgcc tgacttccgt cgtgcagatg gttatgtcac ttctacaaaa
    25621 ccgcttagct acaacggaaa tatcattgaa ggcattaagg tgacctttaa ggatggacaa
    25681 atcgtagata tcactgctga gaagggtgat caggttatga aagaccttgt ctttgaaaat
    25741 gcgggtgcgc gtgccttggg tgaatgtgcc ttggtaccag atccaagtcc aatttctcag
    25801 tcaggcatta ccttttttaa cacccttttc gatgaaaatg cgtcaaacca cttggctatc
    25861 ggtgcagcct atgcgactag cgttgttgat ggagcggaga tgagcgaaga ggagcttgaa
    25921 gctgcagggc ttaaccgttc agatgttcac gtagacttta tgattggttc taaccaaatg
    25981 gatatcgatg gtattcgtga ggatggaacg cgggtacctc ttttccgtaa tgggaattgg
    26041 gcaaattaag gagataatat gttaggaagt atgttcgttg gtctcctagt gggattttta
    26101 gcaggtgcta tgaccaatcg tggagagcga atgggatgtt ttggaaaaat gtttctcggt
    26161 tggatcggag cctttctagg tcacttgctc tttggaactt gggggccagt tttatcagga
    26221 acagctatta tcccagcgat tttaggagcc atgattgttt tagctatttt ttggagacga
    26281 ggaagttaat ttcctaaatc tgatactcaa tgaaaatcaa agagcaaact aggaaactag
    26341 ccgcaggttg ctcaaagcac tgctttgagg ttgcagatag aactgacgaa gtcagctaaa
    26401 aacactgctt tgaggttgca gatagaactg acgaagtcag ctaaaaacac tgctttgagg
    26461 ttgcagatag aactgacgaa gtcagctaaa aacactgttt tgaggttgca gatagaactg
    26521 acgaagtcag taacatatat acggcaaggc gacgttgacg cggtttgaag agatttttga
    26581 agagtatgaa acgaaaagga ggttggtcat tgtactagcc tccttttgat atgatataat
    26641 agttctatga gattagataa atttttagtt gcctgtgcgg tgggaagtcg gactgaggtc
    26701 aaaaacttgc tcaaggctgg gcgcgtgact gtaaatggta aaaaagaaaa atcagctaaa
    26761 ttgcagattg atgaaaaaat agatgagatt cgctttgatg ggcaagtgtt ggagtatgaa
    26821 gagtttgtct actacatgat gaacaagccc aaaggagtta tctcagcgac tgaggatccc
    26881 aagcacagaa ccgttctgga cttgttggat gacttggcgc ggagtaagga agtttttcca
    26941 gtaggacgct tggatattga cacgcatggt cttttgctct tgaccaataa tggtcagctg
    27001 gctcatgttc ttctttcgcc caagcgtcat gtggacaaga cttatcaggc tcaggtcaag
    27061 ggaattatga cccaagaaga tgtggagaca tttatcaagg gcattccgct caaagacttt
    27121 acctgtcagc ccgctagact ggagattctt tccacagatg cagaaaagaa tcagagccaa
    27181 atccgtgtga ccattgcaga agggaagttt catcagatca agcgtatggt gggctactgt
    27241 ggcaaggaag tggtggactt gcaacgattg actatgggaa ctttagtatt agatgagaac
    27301 ctggaacgag gggaatggcg tcgcttgacc aaggaagaat tagaaattct ccgcgctaat
    27361 attatttaat ttagtttaat actcaatgaa aatcaaagag caaactagga aactagccgc
    27421 aggctgtact tgagtacggc aaggcgacgt tgacgtggtt tgaagagatt ttcgaagagt
    27481 ataaattcga aaaaggtgag ggagaatgtc cttacctttt atgtttttta gttgcttcct
    27541 ttgtgaaaag agttataata gactgtagaa taaaaggagg aatctatgaa cgcgattcaa
    27601 gaatcattta ctgataaact atttgccaac tatgaagcaa atgtaaaata ccaagcgatt
    27661 gaaaatgctg ccagccacaa cggaattttt gcagctctgg aacgtcgcca aagccatgta
    27721 gacaacacac ctgttttctc attggattta accaaggaca aggtcactaa ccagaaagcg
    27781 tctggtcgtt gctggatgtt tgcggctctc aacaccttcc gccacaaact catctcgcaa
    27841 tacaaattgg agaactttga gttgtcacaa gcccacactt tcttctggga taagtatgag
    27901 aaatcaaact ggttcttgga gcaagtcatt gcgacttcag accaagaatt gactagccgc
    27961 aaggttagct tcttactcca aacacctcaa caagatggtg gtcaatggga tatggtcgtt
    28021 tccctctttg aaaaatacgg tgtcgtgcct aagtcagttt atcctgagtc tgtttcatct
    28081 agcagcagtc gtgagctaaa tgcgatcctt aataaattgc ttcgtcaaga tgctcaaatc
    28141 ttgcgtgatt tgcttgtttc tggtgcagat caagtgactg ttcaagctaa gaaagaagac
    28201 ctcttgcaag aaatctttaa ctttcttgct atgtcattag gacttccacc acgcaagttt
    28261 gactttgctt atcgcgataa agataacaac tacaaaagtg aaaaaggaat cacaccacaa
    28321 gagttctaca agaaatatgt caatcttcct ttagaagact acgtttctgt tatcaatgct
    28381 ccaactgctg ataaacctta cggaaaatct tacacagttg agatgttggg gaatgtggtt
    28441 ggtagccgtg cagttcgtta catcaacgtt ccaatggagc gcttgaaaga attggcgatt
    28501 gcccaaatgc aagcaggtga gactgtttgg tttggttctg atgtcggcca gctcagtaac
    28561 cgtaaggctg gaatccttgc gacagatgtt tatgactttg aatcaagcat ggacattaaa
    28621 cttactcaag acaaggctgg acgtttggac tatagcgaaa gcttgatgac ccacgccatg
    28681 gtcttgacag gtgttgactt ggacgaaaat ggtaaatcaa ccaagtggaa ggttgaaaac
    28741 tcatggggag acaaggtcgg tacagatggt tactttgttg cctcagacgc ttggatggac
    28801 gaatacacat accaaatcgt tgttcgtaag gaattgctga cagcagaaga acaagctgcc
    28861 tatggagcag aaccaattgt acttgcacca tgggatccaa tgggagcctt ggctaaataa
    28921 aagcatagaa aaaaggaatc agattttaga acctggttcc tttttagttg cttgattaca
    28981 tgatgtgaag aacatgtgcc acaataccca ctgcgaagag ggcaaggata atagtgattg
    29041 gagatacttt tttcttaagt aagtacatgc aaagtaaagt aaggagtagt cctgataatc
    29101 caggaatcaa catatccaag ttttgttgga aagtagtaac tttttcagga gtttgagaca
    29161 atccttgtcc tacttgtgcg aatgcttctt ggataccttt agacccttct ggcaatttat
    29221 cccaatggat ataagccttt tcatctagtt gaactttaga aacatcgaaa gcaaatttaa
    29281 tatttaccca gcgttgaaca aggacagcaa gaatgaacat cccaaggata gaagctcctt
    29341 tagtgatatc ttgaaggata ccaccagaca tatctttagt gatttctgat ccagccttgt
    29401 atccaatctc ttgaacatac cacaagaatg acatacgaat caagttccat gcaacaaaga
    29461 agaggagtgg ccccaagata ttgccagtaa gggcaagtga agcaccgaga gatccaagga
    29521 ttgggcgtac tgtaaaccag aatactgggt caccgatacc agcaagaggt cccatcatac
    29581 cgattttaac cccttggata gcagcgtcat cgatttccac accgttagca cgttcttctt
    29641 caagcgcaag agtaaccccc atgactggag cagctacgta tggatgagtg ttgaagaact
    29701 caaggtgacg ctcaagagca gcgatttgat cttctttttt agtatagagt tttttgatag
    29761 ctggaatgag tgtataagcc cagcccaagt tttgcatccg ttcaaagttc caagaccctt
    29821 gtaagaaggt tgaacgccac caaacttttt tacgatctga tttagttaat tgaagttttt
    29881 cagtcatgat gttttcagtc ctttcttatc ttagtagtct tctaggatat cgccgattgg
    29941 gtcgttagaa gttgcggctc ctccgccacc atttccacca gttttagaaa ggtgaaggta
    30001 gataagagcg atagcaacac cgatagcacc gaatccgatt agagtaatat ctgacacagc
    30061 agcgagaacg aaaccaagag cgaagaatgg ccatacttca cgagttgcca tcatgttgat
    30121 aaccatggcg taaccaacgg caacgaccat accaccaccg atagccatac catctttgag
    30181 ccagtctggc atggcactaa ggatactttg tacagtttca gttggtacca taaggagaag
    30241 agctgcagga agcgcgatac gaagtccttg gaagagtagc gcaatgaaat gcgcacgctc
    30301 cacagcgccg aagtcacctt ttttagcggc agcatctgca gtatgaacca aaccaactga
    30361 aattgtacga acaatcattg tcaagaaaag tccagctacg gcaagaggga tagcaaccgc
    30421 ttgggcaaca ccgataccag tcttggtaaa gtcaccacca agaaccatga taatggcagc
    30481 agcgacagaa gcaagtgcag catcaggagc gatagcagca ccgatatttg accaaccaag
    30541 ggcaatcatt tgaagcgatc caccgaggat aatccctgct tccaagtgac ctgttacaag
    30601 cccaataagg gtacaggcta caagtggttg gtgaaattgg aactggtcga ggatgccttc
    30661 aagacctgca aagaaggcta caacgactac taaaaccata gaaataatag acatgtttaa
    30721 aatcctttca taaataatgg cttatttgac attggctttg ttaatcaagt caaacaaatc
    30781 ttttttagaa tcatttggta ctttacggac atcaaattca acacccaagt cacgcatttt
    30841 ttcaaatgta gcaacatctt ctttgtccat agacaaaacg gtattgacca atgttttacc
    30901 tgttgagtga gccatagaac caacgttaag agtcttgatt ggcacgccgc cttcgatggc
    30961 acgaagggca tcttgaggtg tttcaaacaa gataagggca tgtgtttctc caaaacgtgg
    31021 gtcttttgaa atctcaatca gtttttgaat tggaaccacg ttagccttga cattacctgg
    31081 agctgcttgt ttaatcaatt ctttacgaag gtcgtcttta gccacgttat ctgaagcaac
    31141 gatgatacga tttgcttttg aatctggagt ccaagcagtt gcaacctgac cgtgaagtag
    31201 acgtgtgtca agacgggcaa gattgatttt caatttaccg tctccgataa cagttccttc
    31261 tgggatagca gtttgggcaa ctggagcagc tgcagcgctt gcaacttctt caactggatt
    31321 tagctcttct ggaagagctt tgatgccatc tttggcttct ttaatgatat tagcagcgac
    31381 tttttctaca cctgcagcag cgtccatgag gcgctctgtg taggcttgaa tcaacatcgg
    31441 taagttaagt cctgtgatga tggcaaactt acgctcagga ttttctccca tcacgcgact
    31501 agcttggtta aatggagaac cactccaaag gtcagccaaa actagaacct catcttctgc
    31561 gtcaaatgca gcaacagcgt tattaaactt agcgtataga tcatcaggac cttcatttgg
    31621 cataaaggtc acaacttgaa ccttttcttg ttcaccaaag atcatagatc ctgactgatg
    31681 aatacccgca gcaaattcgc cgtggctcgc aataatgatt ccgatactca ttattgtcat
    31741 tcctcctttt ttgttttagt tttcatttaa gaaaacttta agactgttta agtataaacc
    31801 gttttcataa aaaaagcaac tatttatcat aaaaaattaa aaaattttta tttttgtatg
    31861 ttgatatgtt gagtttttga ggagtaatct tgaattctta tttaaaattt aatataaaaa
    31921 agttggaaga agcttccaac ttttaaaata ggtttctatt agattagtgg gtgaagtcga
    31981 gtaccatacg tccttggatt tggccttttt ccatttcgtc gaaaatggca acagcatctt
    32041 ctactggacg tttttgaaca actgggacta ccagaccttc tgcaccaaat tggaaggctt
    32101 cttctaagtc tttacgagtt ccgacaagag aaccgatgac ttggattcca tcgaggactg
    32161 ttttaacgat gctgagttcc atcatttcag aaggaagacc aacagcgacg acgcgaccac
    32221 cagcacgaat ggagtcaaca gcctggttga aggcaacttt agacacagca gttacgacag
    32281 ctgaatgagc tcctccatca gttttttctt taatgagtcc agctacatct tcaacttcga
    32341 ggccgttaat cacaatgtca gcgcctactt cttttgcaag ggcaagtttg tcattgttga
    32401 tatcgactgc gataacatga gcattgaata cttttttagc gtattgaaca gcgaggttac
    32461 caagtccacc agcaccgtaa agaacagccc attggcctgg ttcaactttt gcttctttga
    32521 tagctttata ggttgttact ccagcacatg tgatagaaga agcttgggct ggatcaagtc
    32581 cgtcaggaac tttgacagca tagtcagcag ttacgataca ttgttcagcc ataccaccgt
    32641 ctactgagta gccagcattt ttcactgtac ggcaaagggt ttcgcgacca gttgtacagt
    32701 attcgcaagt gccacatcct tcaaagaacc aagcaacgct gacgcggtca ccgactttaa
    32761 ggcttttcac atctggagca atctctttaa cgataccgat accttcgtgc ccaagaacac
    32821 gtcctgggac ttgaccaaag tcaccatgag caacgtggag gtcggtgtgg caaacgccac
    32881 agtattcaac ttctacaagt gcttccccag tttcaagtgg acggagtact ttttcttcaa
    32941 tagcaacacc agtgctttct ggatttacaa caacagcttt catagctatc ctccttgata
    33001 ttgaccgaga acagggacaa cgggactgga ttgatttttt gtcaacagag tcgagtgtga
    33061 cgagctctct tgtatttata tgtgaagtat atcacaaaag aaagcctttt ccaaggtttt
    33121 ggagacaaaa agtagaacaa tcgattaact ggttgatgag actgtgaaaa gagcacaaag
    33181 accagcttgc aagctggtca tactcttcga aaatctcttc aaaccacgtc agcttcatct
    33241 acaacctcaa agcagtgctt tgagcaacct acggctagct tcctagtttg ctctttgatt
    33301 ctcattgagt atcagtatag gttataagaa taagttataa gaataagtct tgtagggttt
    33361 tggcaacccc atcttggtcg ttggtaaggg aaatttgctc atctgcataa gggagtagct
    33421 ctggattggc atttttcatg gcataaccct tcccagcaaa agcgagcatt tcggtatcat
    33481 tgtgttcatc tccaaaggca atcaaatctt ttttgtcacg attcattatc ttgagcaagt
    33541 agtccaaagc aaaggccttg ttgacacctt ttggggtaca ttcaaggata ttgagcggac
    33601 ctccccaggt attgatagaa agttgatgct ggtagaaggc gttcatttct tttgccaagg
    33661 catatttgtc actggctctg gtctgcaaga ggatacagtt agggtccttg gtcaccaatt
    33721 caggctggaa ttgatcttca ggctggaaag cttctacacc aaatagtttg ggattggcaa
    33781 tttcttcatt gggatttgta atgtagaatt ttttacgata ttctccagcg ataaaatcgg
    33841 cttgaatgtc ctctgaacgt tgaaccatat ctagcagata ttttttgtct acagtcaaac
    33901 acttttcaaa atcccaaact tggtctggta aatgagtaag ggatccgttg aagttaatca
    33961 taggagtgtc taagcccagt tcacggtaaa aatcttttga catacggtaa gggcgacctg
    34021 tcgtaataat aacctgatgg cctttttcag caactttttt gatggttttt ttggtaaagt
    34081 cagaaatttg actgtctgag ttgagcaggg ttccatctag gtcaactgca ataatttttt
    34141 tcgtcatatg gacctttcta gtgtgttttc agataagatg tcttctatta taacagaaag
    34201 caggcagaaa agcagattcc aaacaaaaaa tgaaattgga ttaatttctt aaacaaatca
    34261 aacaaagaca ttgaaaaagt gaatgataaa tgatataatt ctattattgt tcgtaaaaat
    34321 taaaaggaga ttgatgatgg acaaattatt taaactaaaa gagaacggta cagacgttcg
    34381 tacagaggtt ctcgctggtt taacaacttt ctttgcaatg agctatattc tctttgtaaa
    34441 cccacaaatt ctttcgcaaa caggaatgcc tgctcagggt gtgtttcttg caacgattat
    34501 cggtgcagtt gctggtacct tgatgatggc cttctatgct aacttgccat acgctcaagc
    34561 accaggtatg ggactcaatg ccttctttac ctttacagtc gtatttggac ttggttattc
    34621 atggcaagaa gccctagcta tggtcttcat ctgtgggatt atttcattgg ttattacctt
    34681 gacaaatgtt cgtaaaatga tcattgaatc gattccaaat gcccttcgct cggctatttc
    34741 agctggtatc ggtgtcttcc ttgcctatgt agggattaag aatgctggac ttttgaaatt
    34801 tacgattgat ccaggcaact atactgttgt aggagaaggg gctgacaaag ctcaagcaac
    34861 gattgcagca aactcttcag cagttccagg cttggtcagc tttaataatc cagctgtttt
    34921 agtggctctt gcaggacttg ccattactat cttctttgtc atcaaaggga ttaaaggggg
    34981 aattattctc tctatcttga caacaactgt tcttgctatt gcagttggtt tggttgattt
    35041 gtctagtatc gattttgcta ataaccatgt tggtgcagct tttgaagatt tgaagacaat
    35101 ctttggtgca gctcttggtt cagaaggttt gggagctttg gtttcagata cagctcgctt
    35161 gcctgaaact ctgatggcca ttcttgcctt ctcattgaca gatatttttg acacaattgg
    35221 taccttgatc ggtacaggtg aaaaagttgg tattgtagcg acaaatggtg aaaatcacca
    35281 atcagccaaa ttggataagg ctctttactc tgatttgatt ggaacgacag tcggtgccat
    35341 tgcaggtact tcaaacgtaa cgacttatgt tgagtctgct gctggtatcg gtgcaggtgg
    35401 acgtactggt ttgacagcct tggttgtagc tatctgtttt gcgatttcaa gcttctttag
    35461 cccacttcta gcgatcgtac caacagcggc tacagctcca atcttgatta tcgttgggat
    35521 tatgatgctt ggtagcttga aaaatatcca ttgggatgat atgtctgaag cagttcctgc
    35581 cttcttcaca tctatcttta tgggattcag ctactctatc actcaaggga ttgcagttgg
    35641 tttcttgact tacactttga ctaagcttgt caaaggtcaa gttaaagatg ttcatgccat
    35701 gatttggatt ttggatgcct tgtttatcct taactacatc agcatggcct tataatagaa
    35761 tgacccaggg ggatttcccc ccttttttaa tacaaaggag ataggtgatg aaagagaaaa
    35821 atatgtggaa agaattgttg aatcgtgcag gctggatttt ggtcttttta cttgccgtcc
    35881 ttttatatca ggttccccta gtggttacct ctattttgac tttaaaagaa gtagccctgc
    35941 tacagtcagg gctgatagtt actggccttt caattgtggt tctggctcta tttattatgg
    36001 gagctcgtaa aaccaagtta gctagtttta atttttcttt ttttagagct aaagatttgg
    36061 cacgtttggg cttgagttat ttagttattg tcgggtcaaa tatacttggt tctatcttgt
    36121 tgcaactgtc aaatgagacg acaacagcta accagtctca gattaacgat atggttcaga
    36181 atagttctct gatttctagc ttcttcttgc tagccttgct tgctccaatt tgtgaggaaa
    36241 tcttgtgccg tgggattgtt cctaaaaaga ttttccgagg caaggagaac ttgggatttg
    36301 tagtcggtac gattgtgttt gctttattgc atcaaccaag taatttacct tctttattga
    36361 tttatggagg tatgtcgaca gttctatctt ggacggccta caagacccaa cgtttggaaa
    36421 tgtcgatctt gcttcacatg attgttaatg ggattgcttt ctgtttgttg gctcttgtgg
    36481 tgattatgag tcggacatta ggaatttctg tttaaaagtt tttatgtagg aaccgacctc
    36541 tttctactag ggaaagatga atgcaatcgt gtccatcttt ttctttttat ggtaaaatag
    36601 aaaaataata tgatgaaaat ccttgaggga gtgaccgata tgtcaagtaa agccaatcat
    36661 gcaaagacag ttatttgcgg aattatcaat gtaaccccag actccttttc ggacggtggt
    36721 caattttttg ctcttgagca ggcactccag caggctcgta aattgatagc agaaggagcc
    36781 agtatgctcg atatcggcgg agaatcgact cggccgggaa gtagctatgt tgagatagaa
    36841 gaggaaatcc agcgtgttgt tccagtgatc aaagcgattc gcaaggaaag tgatgtcctc
    36901 atctctattg atacttggaa gagtcaagta gcagaggctg ctttggctac tggtgccgat
    36961 ctagtcaatg atatcactgg tctcatgggt gatgaaaaaa tggcccatgt ggttgctaaa
    37021 gctggagcga aagtggtcat catgtttaac ccagttatgg ctcgacctca gcatcctagt
    37081 tcgcttatct tccctcattt tggctttggt caaaccttta cagaaaaaga gttagctgac
    37141 tttgaaacat tgccaatcga agacttgatg gtggctttct ttgaacgagc actagcgaga
    37201 gcggcagaag ctggtattgc accagaaaat atcctgttgg atccaggaat tggctttggt
    37261 ctgaccaaga aagaaaatct tctcctttta cgggacctgg ataaactaca tcagaagggc
    37321 tatccaatct ttctcggagt gtcgcgcaag cgatttgtca tcaatatcct tgaagaaagt
    37381 gggtttgaag tcaatcctga gacagagctt ggtttccgaa atcgggacac ggcttcggct
    37441 catgtaacta gtatcgctgc gagacagggt gtagaagtgg tgcgcgtgca tgacgtagct
    37501 agtcacagga tggcagttga aattgcctct gccattcgtc tggctgatga agcggaaaat
    37561 ttagatttaa aacaatataa ataagatgaa agaaattgaa aacaatcagt ggattgctaa
    37621 ctaccggacg gatcaaccgc attttggctt ggaacgaatg gtggaactgt tagctttgcg
    37681 tggcaatccc catcttaaac tcaaggttct ccatatcgga gggactaacg gcaagggctc
    37741 gactattgct tttttgaaaa agatgctaga aaagctaggg ctgagagttg gcgtgtttag
    37801 ttcgccctat ctcattcatt acacagacca gattagcatc aatggggaat cgatctcaga
    37861 agcgaggcta gaagctctca tggcagacta tcagtctttg ctggagggag aagcggtcgc
    37921 caatttacag ggcacaaccg agtttgagat tatcacagcc ctagcctatg actactttgc
    37981 ctcagagcaa gtagatgtgg ctatcatgga agttggcatg ggtggacttt tggatagtac
    38041 caatgtctgt cagcccattt tgacaggaat tacaactatt ggcttggatc atgtggccct
    38101 acttggtgac accttggagg ccatagcaga gcagaaggca ggtattatca aacaagggat
    38161 gcccttggta acagggcgta ttgctccaga agccttgact gtgattgacc gcattgcgga
    38221 agggaaagat gcgccgagac ttgcctacgg gacagattat caggttcgtc atcaagaaag
    38281 tgtggtgaca ggggaagtct ttgactatac aagtgctgtc agacaaggtc gcttccagac
    38341 tagcctgctt ggtttgtacc aaatagagaa tgctgggatg gccatagctt tacttgatac
    38401 tttttgtcaa gaagatggtc gagagctagc aagcaatgac tttcttggtc aagccttgga
    38461 agaaacaagt tggccagggc gtttggaaat cgtgtcaaga gatcccttga tgattttgga
    38521 tggagcccat aatccccatg ctatcaaggc cttgttggta accttgcaag aacgttttgc
    38581 ggattatcat aaggaaatcc tcttcacttg tatcaaaacc aaagccttgg gggatatgtt
    38641 ggacttgctg ggagccatgc cagataccga gcttactcta acacattttg cggatagtcg
    38701 ggcgacggat gaaagcgtgc tgaaagaggc agctaagtct agaaatctca gctaccaaga
    38761 ttggcatgat tttctagagc agaatttgac agataaaaaa gaagagaaac aaacagttag
    38821 gattgtcaca ggttccttgt atttcttgag ccaagtgagg gcctatctga tggagaggaa
    38881 gaacgagaat ggatacacaa aagattgaag cagctgtaaa aatgattatc gaggctgtag
    38941 gagagaacgc taatcgcgag ggcttgcagg aaacacctgc tcgtgtagcc cgtatgtatc
    39001 aagagatttt ttcaggtctt ggtcaaacag cagaggaaca tttgtcaaaa tcctttgaaa
    39061 ttattgacga taatatggtg gtagaaaagg atatcttttt ccataccatg tgtgaacacc
    39121 acttcttgcc attttatggt agagcgcaca ttgcctacat tccagatggt cgtgtggcag
    39181 gcttgtctaa gctagcccgt acggttgaag tttattcgaa aaaaccacaa attcaagaac
    39241 gtttgaatat cgaagtggcc gatgccttga tggactatct aggtgctaaa ggagcctttg
    39301 ttgtcattga ggcggaacat atgtgtatga gtatgcgtgg tgttagaaaa ccaggcactg
    39361 caaccttgac gacagtagct cgtggtctat ttgaaacaga taaggacctc cgcgaccaag
    39421 cttatcgttt aatggggcta taaaaagaat ccgtttcaag cggatttttc tagaaaggaa
    39481 tcattatgga tcaactgcag attaaggatt tggaaatgtt tgcctatcat ggtctttttc
    39541 ctagtgagaa agaattgggg cagaaatttg tcgtttcagc catcctatcc tatgatatga
    39601 ccaaggcagc tacagacttg gatttaacag cctctgtcca ttacggagaa ttgtgtcagc
    39661 agtggacgac ttggtttcag gaaacgagtg aagatttgat tgaaacggta gcctataaac
    39721 tggtggaacg tacctttgag tcttatcctc ttgtccaaga aatgaagttg gaactgaaaa
    39781 aaccttgggc accggtgcat ttgtcactag atacttgctc ggtaaccatt catcgccgca
    39841 agcaacgagc ctttatcgcc ctaggaagca atatgggaga taaacaagca aacttgaagc
    39901 aagccattga caaactgcga gctcgtggca tctatattct caaagagtcc agtgtcttag
    39961 cgacggagcc ttggggtgga gtggagcagg atagctttgc caatcaagtg gttgaggtgg
    40021 aaacctggct accagcacaa gacttgttag aaaccttgtt agccattgag tcagagctgg
    40081 gacgggtgag agaagtgcat tggggacctc gtttgattga tttggacttg ctctttgtgg
    40141 aggaccagat cctttataca gacgacctca tattgcctca tccttacata gcggaacgcc
    40201 tttttgtcct tgagtcctta caggaaattg cgcctcattt tatccatccg atattaaaac
    40261 aaccgatccg taacttgtat gatgctttga aaaaatagaa aaactctagt tttcagttac
    40321 ttgcaactga aggctagagt ttttaaaata ggtcattttc ttctgggagg aggatagttt
    40381 ctctaccgtc catgtctaaa accagtactc ttgggggata acgagggtcg aaaggatggt
    40441 taaagtcaaa atcaatggct gtagggaggt gttgacttga aaagtggaag gtaatctttc
    40501 cttggttatt aagcaattga aactcgagtt cttcttccaa ttcaaagaca ttttttaaga
    40561 aatggtcgat aatataccaa aaagagtcaa tgatgtcatc aggcaagctg gtaacaatgc
    40621 caaaactagc agaccgcatg tgggtattgg taaaagccat atctctgtct cctttctttt
    40681 cccttatcat acagcaaata ggattaaaaa tcaagaaaag gtgatttttt gaaaaggatt
    40741 ttaactacag ggaaaaagaa ggaaaaaaat tcccaaaaat ctaccgaagt taataggtaa
    40801 attcccaaat taacttgatt atataacttt cagttatttt gagaagttac cgaaaaatat
    40861 tttttatatc tattgacttt taggggtgaa atttggtatg atagtaggcg gtattgttta
    40921 ccccatttga aaggccccgg aaccttccaa atacttttcg atgggaagga acacccatca
    40981 ccgtaaacaa aaatcgaact atatatagga gaaatcatga acaaaacaac atttatggct
    41041 aaaccaggcc aagttgaacg taaatggtac gtagttgacg caactgatgt accacttgga
    41101 cgtctttctg cagtagttgc tagcgtactt cgcggaaaaa acaaaccaac atttacacca
    41161 cacactgata caggtgactt cgtgattgtt atcaatgctg aaaaagttaa attgactggt
    41221 aaaaaagcaa ctgataaaat ctactacact cactcaaacc acccaggtgg attgaaacaa
    41281 atctctgcag gtgaacttcg ttctaaaaat gcagtacgtt tgattgagaa atcagttaaa
    41341 ggtatgcttc cacacaatac acttggacgc gctcaaggta tgaagttgaa agtatttgtt
    41401 ggagctgagc acactcacgc tgcacaacaa ccagaagttc ttgacatttc aggacttatc
    41461 taaggaaagg aacaataaag tatgtcacaa gcacaatatg caggtactgg acgtcgtaaa
    41521 aacgctgttg cacgcgttcg ccttgttcca ggaactggta aaatcactgt taacaaaaaa
    41581 gatgttgaag agtacatccc acacgctgac cttcgtcttg tcatcaacca accattcgca
    41641 gttacttcaa ctgtaggttc atacgacgtt ttcgttaacg ttgtaggtgg tggatacgct
    41701 ggtcaatcag gagctatccg tcacggtatc gctcgtgccc ttcttcaagt agacccagac
    41761 ttccgcgatt cattgaaacg cgcaggactt cttacacgtg actcacgtaa agttgaacgt
    41821 aagaaaccag gtcttaagaa agctcgtaaa gcatcacaat ttagtaaacg ttaattcgaa
    41881 agaattacta tacttataca gagcaccttt cggggtgttc tttttttata ctttcttact
    41941 aaattggtgc aattgacaca gttgttgcga ctttagtcgc ttacaaatgt ggctgcaacc
    42001 tgacaaggtc agttgcctca aaacgttaat caatacaatt atatcaacgt ttcaaagcac
    42061 tcaagagttt acctcatggg tgcttttttt atgttttttg aaaaagttca cctcaaagtt
    42121 taccttattt tcaccttatt gttttagaga ctgtaaggca aattcaccga cggccatagg
    42181 gactacatta gaagtattga cataaatctg tgttgtacct gtgtcgctat gtattagagc
    42241 ttcagaaatg gcttctaagc tcattcctgc ttgtttagcg agcgttgctc cggtatgacg
    42301 tagtttgtgt ggcgtagcgt gtgtaagctc tgggtgtcgt tttctgatag ttttcatctt
    42361 attattgaga tagtcagaat gtaaaggttt attgatattg cctttggtat ctatgtaagt
    42421 aaagatgaat tgttctggat tactgatgat accaaacttt gccagttcat atttttgttg
    42481 ttttttccat gaagtcagaa gttgaagtaa gtcattagaa atagagaaaa tgggaaatgt
    42541 ttccacaata aaacgcatat tatcagatgg ttaatgcgtt ttatatttgg gtttgaggct
    42601 tataaaatag gataagcgta ctgaaaactt agatatgaca aacaaaataa aatttttaaa
    42661 agctacatag aaacatggta taatgtatct aaaggaggta gcatatgtcc ttagatatag
    42721 ataaagaaaa gatgacaatc atgggaatag cttttgaaaa ccgatccgtt tttaagagtg
    42781 tttggtatgc tttaagtaca aatatgattg aaggatggcg acctacggtt agtgatgttg
    42841 aaaaattacg agatgaagct cttgctctgg ggatgactta atgaagcgca actgttatga
    42901 atcttatgac tatgttgatc cggataattt atataccgat ccgggttctt cagttctaag
    42961 aaataagcag gaaggaaaaa agcacgcgag cttgaatatc gtatgattgc tagtaagagt
    43021 ttgaaattat ttatcaatcc tatcttagtt aatagtttaa aagtagtttg tttcacaact
    43081 gcataagctt agaaaagttg aaaagtgata actctttaat aaactcttgg tttgttaaaa
    43141 gatattttgt ccactacatt tttttcaatt gactattatt tgtcatagat ataacagata
    43201 gaacattgca ttcagaataa tgaaaaacgc atcaaaaatg gtgcgttctg tttttatgct
    43261 accataaaag gaggaaaaca aattgaaaat ttttaaggga gagttttatc gaatctctgt
    43321 attaacagac aagctagtaa ggttagaata ctctcgaact ggaaattttg aggatagaac
    43381 gacacaactt atctataata gagattttgg ccaagtttcg ttagattata tcgagacatc
    43441 aaatgtacta gatattatga cggactattt tcatctgcac tttaataaag gagaatttaa
    43501 cgccgaaaat ttatttatag aattaaaagg aaattttgcc gtatatggta gtcgctggta
    43561 ttttggtgaa tctattgaaa cgttaaaagg aacagctcgg actctggata aggcagatgg
    43621 agcaatctcg ttagaagatg gaattattag ccgaaatggt atagccttat tggatgattc
    43681 tcaaggattt atttgggatg aacaatctgg ttatattgag agagaaaatc aaattgacct
    43741 gtatttcttt gcctatgggc atgattatag aggagcaatc agagactttt accatttgac
    43801 tggttcaaca cccttgttgc caagatatgc tttaggcaat tggtggagta gatattggcc
    43861 ttatacgtcg gatgaatact tggatttaat agacagattt gaaacagaga aaattccatt
    43921 atctatcggt gtgttagata tggattggca tataactgac attccagctc gttttggaag
    43981 tggctggaca ggatatagtt ggaatagaaa cttaatacca aatccagaac agttattgca
    44041 acaacttcat gatagaaagt taaaactctc cttaaatgtc catcctgctg atgggatacg
    44101 ggcttatgaa gaagcttatc ctcaaatcgc aaaacgattg gggttaaatg tagaactaga
    44161 agaacctgct atttttgatt tttttaatcc ctcttttagg gaagcctact ttaaagatgt
    44221 tcattatgag ttagaaaagc agggagtaga tttttggtgg attgactggc aacaagggac
    44281 acaaggtatg ctagatccac tttggctttt aaaccattat cactatcagg atagttgtaa
    44341 aaatgcagaa ggtggtttga ttttatcaag atatgcaggt cctggtagtc accgctaccc
    44401 tgttggtttt tcaggggata ctattattag ttggaattcc ttaagatttc aaccctattt
    44461 tacagcgaca gcatctaata tcggttatag ttggtggagt catgatatcg gtggacatat
    44521 gttgggggat tatgacgaag agctacaaac tagatggcta cagtttggtg tttttagtcc
    44581 gattactcga ttacatagtt ctagaagtcc ttttaatagt aaagaacctt ggtttttttc
    44641 agaaacaaca tctaagatta tgaagaaata cctccgtttg agacatcaga tgattcccta
    44701 tctatatacc atgaatgtaa agacacatga ggaaggtgcc ccattaatca gtccaatcta
    44761 ttatttctat ccagagaatg atgagagcta taatgttcca aatcaatact tttttggaac
    44821 agaactgatg gtggctccca ttgtagaaaa gatggatttg acattccaat ctgcaaaagt
    44881 agatgtatgg ttccctgaag gtgaatggta tgactttttt tcagagaaaa aatacacagg
    44941 tggtgtgaag ttaagtgttt atagagacat ctcgactacg cctgtgtttg caaaaagtgg
    45001 tgcaatcatt cccttggttg gttctgagat aggtatgggt gttgatttac ctgaagttgt
    45061 agattggtat gtattcccag gaaaacaaca ttcttttgaa atgcttgaag atcaaaatgg
    45121 tcaaagatat aaaacaagat tatcaatcga ttgggaaatg ggaatggtag agttagcatt
    45181 acaaggagat tctagtatcg ttccaagcaa tagaaaacat agaattcatt ttaaaggaac
    45241 gaatgtgtct ataattgaat tgccaaataa gaatgataca gctaaatttg aatggaaaga
    45301 taataaaagg acatctttaa atgacgaagt ttttagacta ctaaagacag cttctcttcc
    45361 atatgaatta aaagatagat tgttaaatca attcatcaat gcgaaaaact ctcatgactt
    45421 aatgaatatc ttgcatcatc aggataagga attgagaggg cgtttgttgg aaatgatatt
    45481 tactagccaa aactaattga aataattaga aagtcatttg tttagtgtat atgaagattc
    45541 tgtatggtat tcgttatcac tggtcacatt gaaaatgtga aataagcacc tacaacatag
    45601 actttacaac acttcatgtt ttatatcatg gggtgttttt ctatttaagt caaagcccat
    45661 caaaaaatct cactattcat caggttaaag gaaacctaat catagtgaga ttgtgaatta
    45721 tagtagagtt tgtatcgcct cttcaatttg ttttgggtct gtttttgaag aaaagcgttc
    45781 aaagacttgc ccatctcgac cgatgagaaa cttagagaaa ttccattcga ttcgttttcc
    45841 tagtgggccg gatttctggt cttttaacca gacatagaga gggtctgctt ccttaccgtt
    45901 gaccttaatc ttggcaaaac gtgggaaggt ggtttgaaaa tgtaggctac agaaggcgtt
    45961 gatttcctct gcgctgccgg gtgcttgtcc cataaactga ttgcaaggga aatccaatat
    46021 ttcgaagccc tgagcttgat agcgttcata gagttcttga agtccctggt actggggcgt
    46081 taaaccacat ccagtagcag tgttgacaat caagagaact ttgccacgat agctatccaa
    46141 gggagttgct tggttgtttt ggttcaaaac ggaaaaatca taaagtgaag tcatgagggc
    46201 ctcttttctt tttctacatt gtaacagttt ttactttttt cgtcgagtcg gatggaagat
    46261 attgttgaag aataagtctt caatatgata gaaaccgttt acaaataact ttaaaaatgg
    46321 tatgatttaa ttaaaccggt ttacttagtt gctataaatc tctaatttga gatttagttc
    46381 ctattgattt tacaatatgt ttattggagt gtatacatgc aaacaaaaac aaagaagctc
    46441 attgtgagtt tgtcttcact tgttttatca ggatttttat taaaccatta tatgacagtt
    46501 ggagcggaag aaacgactac gaataccatt cagcaaagcc agaaggaagt tcagtatcag
    46561 caaagggata caaaaaattt agttgaaaat ggtgattttg gtcagacgga ggacggaagc
    46621 agtccgtgga caggaagcaa agctcagggg tggtcagctt gggtagacca gaagaatagt
    46681 tctgcagatg cctcaactcg agtcattgag gctaagggtg gggctatcac tatctcaagc
    46741 catgagaaat taagggcagc ggttcaccgt atggttccta ttgaagctaa gaaaaagtat
    46801 aaactgcgtt tcaagattaa aacagatcat aaagtcggga ttgccaaggt tcgtatcatt
    46861 gaggaaagtg gtaaggacaa gcgattgtgg aattctgcaa cgacgtcagg aacaaaggac
    46921 tggcagacca ttgaagcaga ctatagcccg actttagatg ttgataaaat caagctggag
    46981 ttattctatg aaacaggaac tgggactgtt tcctttaagg atattgagct ggtagaggta
    47041 gcagaccagc tttctgagga ttctcaaaca gataaacagc ttgaggaaaa gattgattta
    47101 ccaattggaa aaaaacatgt tttttctctt gcggactata cttataaggt agaaaatcct
    47161 gacgttgctt cagtcaaaaa tggaatttta gaacctctta aggaagggac aaccaatgtc
    47221 attgtcagta aagatggcaa ggaagtgaaa aagattcctt tgaagattct agcctctgtt
    47281 aaggatgcat acacagcccg tttggatgac tggaatggca tcatcgctgg gaatcaatac
    47341 tatgattcta aaaatgaaca gatggccaaa ttaaaccagg aattggaagg aaaggtagct
    47401 gatagcctat ctagtatttc aagtcaggcg gaccgcacct atttgtggga aaaattttca
    47461 aattataaaa tgtctgcaaa tctgactgcc acttatcgga aattggagga gatggccaag
    47521 caagtgacca atccttcttc tcgttattat caagatgaaa ctgtcgttcg aacagtcagg
    47581 gattccatgg aatggatgca taaacatgtc tacaatagtg aaaagagcat tgttgggaac
    47641 tggtgggatt atgaaatcgg tacacctcgt gccatcaaca ataccttgtc tctgatgaaa
    47701 gaatacttct ctgatgagga aattaaaaaa tatacagatg tgattgaaaa atttgtacca
    47761 gatcccgaac atttccgaaa gacgactgat aacccattta aggctctagg tggaaactta
    47821 gttgatatgg gaagagtaaa agtaatagct ggtttactgc gtaaggatga tcaagaaatt
    47881 tcttctacca ttcgctcgat tgagcaagtg ttcaagttgg tagaccaagg tgaaggtttt
    47941 tatcaagatg gatcctatat agaccatacc aatgttgcct atacgggtgc ttatgggaat
    48001 gttttgattg atggcctgtc tcaactgttg ccagtcattc aaaagaccaa gaatccaatc
    48061 gataaagata aaatgcaaac catgtactac tggattgata aatcgtttgc tcctttgctg
    48121 gtgaatggag agctgatgga tatgagtcgt ggacgctcga tcagtcgtgc aaatagcgag
    48181 gggcacgtgg ccgcagtaga agtactaaga gggattcacc gaatagcgga tatgtctgaa
    48241 ggagaaacca aacaacgttt gcagagtctt gtgaagacca ttgttcaatc ggatagttat
    48301 tatgatgtct ttaagaattt gaagacttat aaggatatca gtttgatgca atccttgtta
    48361 agtgatgcag gagtcgcaag tgttccaaga acaagttacc tatctgcctt taacaagatg
    48421 gataaaacag ccatgtacaa tgcagagaaa gggtttggat ttggcttgtc actcttttcc
    48481 agtcgtacct tgaattacga acacatgaac aaggaaaata aacgtggttg gtatacgagt
    48541 gatgggatgt tctatcttta caatggcgat ttgagtcact atagcgatgg ctactggcca
    48601 acagttaatc catataagat gcctggtaca acagagacgg atgctaagag agcggatagc
    48661 gatacaggta aagttttacc gtctgctttc gttggaacga gcaaactaga tgatgccaat
    48721 gcgacagcaa ccatggattt caccaactgg aatcaaacat tgactgctca taagagctgg
    48781 tttatgctaa aggataagat cgccttttta ggaagcaata tccaaaacac ttcaacagat
    48841 actgctgcaa ctacaattga ccagagaaaa ctggaatcaa gtaatccata taaagtctat
    48901 gtcaatgata aagaagcctc ccttacagaa caagaaaagg attatcctga aacccaaagt
    48961 gtctttttag aatcatccga ttcgaaaaag aatattggtt actttttctt taagaagagt
    49021 tcaatcagta tgagtaaggc tttgcaaaag ggagcctgga aggatatcaa tgaaggacag
    49081 tcagacaagg aagttgaaaa tgaatttctt acgattagtc aggctcataa gcaaaatgga
    49141 gattcttatg gctatatgct catccctaac gtgggtcgtg ccaccttcaa tcaaatgata
    49201 aaagagttag aaagtagcct catcgaaaat aacgaaaccc ttcagtctgt ttatgatgcc
    49261 aaacaaggag tttggggcat tgtgaaatat gatgattctg tctctactat ttccaaccaa
    49321 ttccaagttt tgaaacgtgg agtctatacc attcgaaaag aaggggatga atataagatt
    49381 gcctactata atcctgaaac ccaggaatca gctccagatc aggaagtctt taaaaagcta
    49441 gagcaagcag ctcagccaca agtacagaat tcaaaagaaa aggaaaaatc tgaagaggaa
    49501 aagaaccatt cggatcaaaa gaatctccct cagacaggag aaggtcagtc aatcttggca
    49561 agtctagggt tcttgctact tggggcgttt tatttattcc gtagaggaaa gaacaactaa
    49621 tttgttcatc atctagatga attacatgaa actgtggaaa atagaaaaaa tccgaagaag
    49681 gccaaatatc ttcggatttt tcttttaaaa aaggataagt tgatacttat cctttttact
    49741 tcattatgtg actatagcgt tttgaaacta gaataggaca tcataactgc taaaagattt
    49801 ctataaattc atttgatttt cctaatcaat ttgttcgtat cctatttcac tctactatat
    49861 tagtccgtct cgcacctcac ggagcgagac aaaacaacca cccgctatgc gggtgcgcgt
    49921 cgaaggttat accaaaaaaa ctccaaacgc gatacaataa aggtgttcaa cccacttgta
    49981 aagcgaaagg agaaaaaata tggcacaaaa ggctcatagt ttatctcaca caaagtggag
    50041 gtgttctatc acattgtgtt cacccctaag tatagacgaa aagtcatcta taatcaatat
    50101 agaagtagtt taggcgaaat atttcatcgc ttgtgtagtt ataaaggtgt tgaaatgatc
    50161 gagggtcact taatgccaga ccatgtacac atgttagtca gtattccact aaggctaagt
    50221 gtttcgagtt tcatgggtta tttaaaaggc aaaagtgcac tcatgatgtt tgacaaacac
    50281 gccaatctca agtacaagtt tgggaatcgg catttctggg cggaaggtta ttatgtaagt
    50341 ccagtagggc ttaatgaaga cacaattaag aaatatagtc aagattaaag aaattatcga
    50401 gtggatgatt tcttcacgag tatgaaaatt tgagaactag taaagtatga tatatagtaa
    50461 aatgaaataa gaacaggaca aaatcgatca ggacagtcaa atcgattttt aacaatgttt
    50521 tagaagtaga ggtgtactat tccagtttca atctactata gcactagata aattgagtgt
    50581 aaaagaatat gaggatccct ttagggatag tggtaagtaa taccaaagtc tcttaaagag
    50641 gcaagtgacg agtcaagagc aataaggctt gaacaacgtg aaagccagcg tctttaggcg
    50701 ctggctgatg atttgggctt atagctctga gataaaccac ccgttagacg ggtagttatg
    50761 attttatctg agtgtaacat actgttgggc aatctcgctg atgcggtcaa agttgccttg
    50821 ggaagcgagt ttattgagtt cgccaccaat tccaacggca tctgcaccag cagcgaacca
    50881 ttgagggatg ttgtttagac cgactcctcc ggttaccatt acggaaactt gtggaatcgg
    50941 tgccttgact gcagagatat atgctggact gagagtacta cctgggaaga gtttgatgat
    51001 ttcactaccg gcttcaagtg cagtcgtgat ctctgtgagg gtaatacagc ctggaatgta
    51061 cggtgtgctg tagagattgc acattttcgc agtttcagca tggaaagatg gagaaacaac
    51121 gtaatttgct ccggctagaa tggcatctct agcagttacg gcatcaagca cagtacctgc
    51181 accgatacaa acactctgat cgtcctgata caagtctaca agttccttga tgatttgtcc
    51241 tgcatactga ttggtatagg cgatttcaat agctttgata ccgcccttga tacaagcaat
    51301 cgaggcttgt agtccttctt cctttgtatt tcctcgaatg acagcaacaa ttttctgttt
    51361 ttttagttca ataattgtat ctgatttggt catgtaattc tcctaacgaa tgatatcttg
    51421 tgcatttgcc agtaaatttt caatactagt tgcggaagtg gagagatggt ctcctggaag
    51481 agtgcatttg agagttgcgc tcgcaactgc aaagtcaatg gtagttttta gggaggaatg
    51541 atggagtagt tggtaaaggg caccagatat aaaggcatcc ccgctaccaa ttcgttgata
    51601 gactgcagtt tttagttgga cagactcttc aaatcgttct tccagagcat aggcttgata
    51661 gacatttttg tcttgctcat cactagagct gagggtatgg aaaatggcct tgaaaccata
    51721 ggcttctttt aaaagtcgca tgcgattttc cacctcttct aggctagcac tgtctcttgg
    51781 aaacatctct agattttggt catcaatcat gagaggatca ataccgaagc aatagtcagt
    51841 aaaacgtgca aacttagaaa attcatactt ggcttctagg actgaaatca tctttgttct
    51901 cagattgaga tccattgaaa cgacaattcc tcggcgcttg gcttcttcca agagtaggag
    51961 aaggatcgca cggacctctt gaccgatagc tacggtgatt ccactaaaat gaaaatggct
    52021 aatcccctga aagagagaat ccatatctag catgtttggc cgaatctggc tgatactcgt
    52081 atgcttacga tcgtagaaaa cttcactttg acgacaacca aagccgttct ccaaatagta
    52141 gaggccgatt cgatcgccaa gccgacagat tgaactggta tcgatttggt gctgtttcaa
    52201 gaatgtgaga aaacgatctc caatctcgtt ggcagggagt gcggtaaaga ctttcgttga
    52261 gatacccagg gcttgcaaat tacaagcgat gttaatttct gatccgccaa aataagtcga
    52321 actggcaaca tgatctccga tactggtggc atctaatggt gagattcgaa ttaatggttc
    52381 gccaaacagt aatattttcg tcatccatta gttcctttct ggtttagaat agctttactt
    52441 ggctaatact tctttcaaat aagcagcgac ttcatcatct tggcagtttt caaagaagta
    52501 ttcttggaag tatttgccag atatggtgtc tttgaggaag tcttggtcga ttgttttaag
    52561 gatggtcatc aaatcattgt gggtgatttg tttcacctcg tttaagatac gagcgttgcg
    52621 ttgttcaggg attacacgtt ctcttgggta gccaccgccc atttcttcag caaataagcg
    52681 ttcaaacatc aatttgaggg tcagttctgc cccccagcca aatcctttgg cataagggat
    52741 agacaaggcg ttaccaccat tgatttgaga ataaaggtaa gcgtcagttg ggtccactgc
    52801 tagaccacag acaacaccag ggaagctgtt taaagcaagc atagccccta cacccgtacc
    52861 acagccggta acaacaaagt caactgcctt tgtatttaaa aggatggcag ccattagtcc
    52921 gttctgcaca taagttaatt gactttctcc ttcttctcca cgcataccat agttaaataa
    52981 ttggtagtct tttttatctg tcgcttcttt tagactatcg taaataatgt gattcttgct
    53041 agcttgacta ttttcattga ttaatgcgat tttcataaga tcttctttct atttttactc
    53101 aggttgtttt ccgatgtagg ctaagatacc gccatctaca tataggatgt ggccattgac
    53161 aaaattgctg gcatcactag cgagaaagac agcagggccc atcaaatctt caggatttcc
    53221 ccaacgtgca gcaggtgttt ttgcaatgat gaactggtca aatgggtggc gagaaccatc
    53281 ttcttgcaac tcacgaagag gtgctgtttg aggagtggca atataacccg gtccaattcc
    53341 gttacattgg atattggctc caccgtattc agacgcaatg ttgcgggtca acattttcaa
    53401 gcccccttta gcagcagcat aagcgctaac tgtttcacgt cccagttcgc tcatcatcga
    53461 acaaatattg ataatctttc catgcccttt ctttatcata gaaggaataa ctgcctttga
    53521 aacgataaat ggtgcgttta agtcaatatc gatgacctta cggaaatcag cggcgctcat
    53581 ttcgcacatt ggaactcggc ggataatacc agcgttatta acgaggatgt caatgacacc
    53641 aacctcttgt tcgatttgct tgaccatggc ttggataccg tcctcgtctg tcacgtcaca
    53701 gacatatcca tgggcttcga tgccaacttc acgataagct tcaatccctt tattgaccag
    53761 atcttgattg atatcgttaa agacaatagt agcgccggct tcagcgtagg atttggcaat
    53821 agcaaatcca attccataag aagcgccggt gatgagagca atttttcctt ttaaagaaaa
    53881 ctgctcaatt gagaatgatg tatttgtcat ttgatacttc ctttcaaatt tgttaagact
    53941 tctctataac caaattatat tctagtaaac cggttttgtc aataaataaa tatttttatt
    54001 tataaaaaga tttattttcc ttaaaaatgg aatattttga aatttctgtc tattaaaatg
    54061 gttaaaaaga taatgaaagc gctaatattt ttttaaaaaa actattgaca agataaaaat
    54121 aaaggattac aatggggaat ataaagtaaa ccggtaaacc taaacaagaa aggagaaaag
    54181 atgaaaattg tacttgtagg gcatggacat tttgctacag ggatttatag ttctttacaa
    54241 ttgattgcag gcaatcaaga aagtgtggag gcgattgact ttgtggaagg aatgtcagca
    54301 gatgaactca agcaaaaaat cttacttgca atttcaaatg aagaagaagt tttaatccta
    54361 agtgatctct tgggaggatc gccattcaag gtttcttcta ccataatggg agaaaatcca
    54421 gccaaaacaa tgaatgttct ctcgggtttg aacttagcca tgttaatgga agcagtcttt
    54481 gctagaatgg ctcatagctt tgatgaggtt gttaataaat cagtagtggc ggcccagggc
    54541 ggagtcgtaa atggtaaaga attgttttca acggatgcag aggaagagga agaagatttc
    54601 gaatcgggta tttaaagggt aaaagaatga taaaaaaggt tacgattgaa aaaataaaat
    54661 cgcctgagcg cttcttagaa gtaccacttc tgacgaaaga agaagtcggc caggcaatcg
    54721 ataaggttat tcggcagtta gaactcaacc ttgactattt caaggaagat ttccctacgc
    54781 cagctacctt tgataatgtc tatccaatca tggataacac ggaatggacc aatggtttct
    54841 ggacaggaga actgtggttg gcttatgaat acagtcaaca ggatgcattt aaaaacatcg
    54901 ctcataaaaa tgttctttct ttcctggatc gtgtcaataa gagagtagaa ttggatcacc
    54961 atgatctcgg cttcttgtac acaccgtctt gtatggctga atataagata aatggagatg
    55021 gagaggctag agaagcaacc ttgaaagctg cagataagtt gattgaacgc tatcaagaaa
    55081 aaggtggttt tattcaagct tggggagact tgggcaagaa agagcattac cgtttgatta
    55141 tcgactgctt gctcaatatc caactcttat tctttgctta tcaagaaaca ggcgatcaaa
    55201 aatactacga tattgcagaa agtcatttct atgcttcagc taataatgta atccgtgatg
    55261 acgcttcgtc cttccacacc ttctattttg atcctgagac aggtcaaccc tttaaaggtg
    55321 taacgagaca agggtatagt gatgattcat gctgggcacg tggtcaatca tggggagtct
    55381 atggtattcc tttgacttat cgtcacttaa aagacgagtc ctgctttgac ttgtttaagg
    55441 gtgtgaccaa ttatttcttg aatcgtctgc caaaagatca tgtgtcctat tgggatttga
    55501 tttttaatga tggtagtgat caatcacgag attcttcagc aacagctatc gccgtctgtg
    55561 ggattcatga aatgctaaaa catctcccag aggtggatgc tgacaaagat atttataaac
    55621 atgctatgca tgccatgctt cgttccttga tcgaacatta tgcaaatgat caatttaccc
    55681 ctggtgggac aagtctcctc cacggtgtgt actcatggca ttcaggtaaa ggagtggatg
    55741 aaggcaatat ctggggtgac tactattacc tagaagccct tatccgtttc tacaaagact
    55801 ggaacctata ttggtaggag gagaaatatg acaatgccaa atattattat gacccgtatc
    55861 gatgaacggt tgattcatgg acaaggacaa ctttgggtaa aatacctagg ttgtaatacg
    55921 gtcattgttg ccaatgacga agtaagcacg gacaagatgc aacaaactct gatgaaaaca
    55981 gttgtgccag actcagttgc catgcgtttc ttccctttgc aaaaggtgat tgatatcatt
    56041 cacaaggcta atcctgctca aacgatcttt atcgttgtaa aggatgtgaa ggacgcttta
    56101 accttggtag aaggtggtgt ccctatcaaa gaaatcaata ttgggaacat tcacaatgct
    56161 actggtaaag agcaagtgac acgctccatc ttcctgggtg aagaggacaa ggcagccctc
    56221 aaggaattaa gccaaactca tcaagtaaca tttaatacga aaacaactcc aacaggaaat
    56281 gatggagctg ttcaagtcaa cattatggac tatatttaaa agaggagatc gttatgtcga
    56341 ttaatgtatt tcaagcgatt ttaattggat tatggacagc tttctgtttt agtggaatgc
    56401 tattaggaat ttacaccaat agatgtattg ttctgtcatt tggtgtcgga attattctag
    56461 gtgatctgcc tactgctctt gcaatgggag ctattggtga attggcttat atgggattcg
    56521 gtgttggtgc tggaggtact gttccaccaa acccaatcgg acctggtatc tttggtacct
    56581 tgatggctat cactagtgct ggtaaagtca gtccagaagc ggctcttgcc ctctctactc
    56641 cgattgctgt ggcgattcaa ttcttacaaa ctttcgccta cactgtacgt gctggtgcgc
    56701 ctgaaacggc gatgaagcac ttgaaaaacc ataatttgaa gaaattcaag ttcactctaa
    56761 atgcaacaat ttggttgttt gcctttattg gatttacctt gggttgcttg ggtgcccttt
    56821 caatggatac cttgttgaaa ctcgtagact acattccacc ggtattgctt acaggtttga
    56881 cagttgctgg taaaatgctc ccagctatcg gttttgcgat gatcttgtca gtgatggcta
    56941 agaaagagtt gattcccttt gtcttgttgg gatatgtttg tgcagcttat ctaaacatcc
    57001 caacaattgg tattgcaatt gtaggtacta tctttgcttt gattgaattt tataacaagc
    57061 caaaaacagc ggatcatgtg gtagaggagg aagcacacga tgactggatc taacaaatta
    57121 acaaaacgtg attatcttaa aacgtctttg cgggcattct tttgtcaaaa tggatttaac
    57181 tatagtaact atcaagggtt gggatatgcc aatgtgatgt atcctgcttt gaaaaaacac
    57241 tatggagaga atcaggaagg tttctaccaa gccttggaag aaaactgtga attctataat
    57301 accaacccac acttcctgcc ttttattacc agcttgcatc ttgtaatgtt ggaaaatggc
    57361 cgtccggcaa aagaaacacg tagcatcaag atggccttga tgggaccatt agcaggtatt
    57421 ggggattctc tctctcaatt ctgtttagct ccattgttct caaccatcgc agcttcgttt
    57481 gctcaagaag gcttggttgt cggtccaatc ttgttcttcc ttgcgatgaa tacgatttta
    57541 acagcgatta aattgtcaac tggtctgtat ggatacaaac taggaacgac tgtgattgat
    57601 aaactaagcg agcagatggc aacgatttct cgtattgcca atattattgg tgtaaccgta
    57661 attgctggtt tggcagcgac atctgttaag attatggtac cgatcacctt tgctgcaggg
    57721 gaagttaaag cagacgctaa acaaagtatc gtaagtattc agggaaagct tgataaggtt
    57781 gctccagctc ttctaccagc cctatttaca cttttagttt actacttgat caaagaaaag
    57841 aaatggacaa catataaact cgttatttta acagttatca tcggaattat cggaagctgg
    57901 cttaagatta tagcttaatg gatacaacat tgttttatgg aatagtgatt gtcttggcag
    57961 tgagtcccct tttactgtca agctttcatt ctattcgtca gcaaaagttg cttcgcaaac
    58021 agatggagca acgacaagag tatttagctt ctttaacatc tggtgatgaa gtgttgttat
    58081 tgtctggaat tcatggaaaa atcatttcta tccaagatga cttggtctcc ttgcagattg
    58141 caaaaggtgt ggtcatctat gtagaaaagg aaagtgtaat gggaaagaca aaagaactgc
    58201 tttttaagta gttctttttc tggtaataga taatgaaaga agaaagaaga caattttttg
    58261 aaagaatcga tggaaaccaa tgtcgtgatt atatcttgtc ccactgttca aaagactatg
    58321 agaaagtcaa gagttccctt gaacgcttga tggacaatcg ttttatgttt gatagtcctt
    58381 gggatatgga gccttgttca aaaatccatc aaatccagcc gatggtatgg gatcaagtat
    58441 ttgaagatga tccagaatgg tcttatatgc tcaatcgaca agaatatctc ttgcagttta
    58501 tgatagggta tgtggtagaa ggagataagg actatattca aaagtgcaag ttctttctat
    58561 ttgattggat tgagcaggtg agagaatttt ctcctcaatc cttgatgact agaaccttgg
    58621 ataccggtat tcgttccttt acttggttga aactactctt gctcctcttg aaatttgact
    58681 tgctagagga gaaagaacta gagaaaattt tggtcagtct agaaaagcag attgacttta
    58741 tgaaaagcca ctatcgcgcc aagtacaccc ttagtaactg gggaatttta caaacaattc
    58801 cgatgcttgc tatctatcat ttctttgcag ataagatgga cctagaagaa gcttaccatt
    58861 ttgcttcaga ggagttgaaa cagcaaattg agacacagat tttaggagat ggaagccagt
    58921 ttgaacagtc gattctctat catgtagagg tttataaagc cttgctggat ttgtgtctct
    58981 tgcttccaga cttgcaagat agttaccaag agttgctgga aaagatggcg acctatattc
    59041 aaatgatgac aggcttagat ggacgaactt tggcttttgg tgatagcgat tctacagaaa
    59101 cgacagaaat gttgagcctg tctgctgtgg ttttgaacaa ggaagacctt cttaacggtc
    59161 tggatgttaa agtcgatttg cttagcctct tgttcctggg gcgagaaaag gtcaagcgac
    59221 tgcaggaatt tgaaaagcga gcttggcagc ctaagttcat gagctttgaa gactctggac
    59281 atgtctgcat taaggatgaa catcgttatc tatttttcaa aaatggtccg ctaggaagtg
    59341 cccatagcca tagtgacgag aatagttttt gcttacagta tcaaggccaa cctattttca
    59401 tagatgctgg gcgttattct tatcgggaga tatatgaacg ttatctctta aagagtgctt
    59461 ggagtcattc gacctgcatt gtagatggga aagctccgga aagaatcacg ggatcctggg
    59521 aatatgaata ctatcctcac tccctgtttt gtcaccataa agaaagggag ggaatgcatt
    59581 atattgaagg ggcttattgg tcagcagaac ctgatttgcc ttatcttcac aggagaaaaa
    59641 tcctcatgtt ggtagaggat gtctggctct tggtagatga catcaggtgt caaggtcagc
    59701 atgaggcgtt gactcagttt atccttgaca aggatgtgac ctatcaagat gggaaaatca
    59761 atcagttgag actatggagt gaagttgatt ttgatttgga agataccatc atttctccta
    59821 aatacaatga gcttgaaaga agtagcaaac tcaccaagcg ccaattcttt gagaatcaga
    59881 tgctggatta taccatcatt gcgcatgaga gttttgaaat catccgtcat tctgtctacc
    59941 agacagatga tcgtgaagtg gaaaatgctc tggcttttga agtgaaaaat gacgaaacag
    60001 acaagctgat tctgttatta agcgaggata tttgtgtagg tgaaaaattg tgcctcgttg
    60061 acggaacaaa aatgcgtgga aaatgtctag tatatgataa aataaatgag agaatgattc
    60121 gcttgcagtg ctagaaatag gcattttgaa tagtgaatat gttataataa gtattagtag
    60181 gaggtgtttt agattggaga agaaactgac cataaaagac attgcggaaa tggctcagac
    60241 ctcgaaaaca accgtgtcat tttacctaaa cgggaaatat gaaaaaatgt cccaagagac
    60301 acgtgaaaag attgaaaaag ttattcatga aacaaattac aaaccgagca ttgttgcgcg
    60361 tagcttaaac tccaaacgaa caaaattaat cggtgttttg attggtgata ttaccaacag
    60421 tttctcaaac caaattgtta agggaattga ggacatcgcc agccagaatg gctaccaggt
    60481 aatgatagga aatagtaatt acagccaaga gagtgaggac cggtatattg aaagcatgct
    60541 tctcttggga gtagacggct ttattattca gccgacctct aatttccgaa aatattctcg
    60601 tatcatcgat gagaaaaaga agaaaatggt cttttttgat agtcagctct atgaacaccg
    60661 gactagctgg gttaaaacca ataactatga tgccgtttat gacatgaccc agtcctgtat
    60721 cgaaaaaggt tatgaacatt ttctcttgat tacagcggat acgagtcgtt tgagtactcg
    60781 gattgagcgg gcaagtggtt ttgtggatgc tttaacagat gctaatatgc gtcacgccag
    60841 tctaaccatt gaagataagc atacgaattt ggaacaaatt aaggaatttt tacaaaaaga
    60901 aatcgatccc gatgaaaaaa ctctggtatt tatccctaac tgttgggccc tacctctagt
    60961 ctttaccgtt atcaaagagt tgaattataa cttgccacaa gttgggttga ttggttttga
    61021 caatacggag tggacttgct tttcttctcc aagtgtttcg acgctggttc agccctcctt
    61081 tgaggaagga caacaggcta caaagatttt gattgatcag attgaaggcc gcaatcaaga
    61141 agaaaggcaa caagtcttgg attgtagtgt gaattggaaa gagtcgactt tctaaaatga
    61201 aggaaaatga cttgcaatct ctgttaagaa ataaaataat cccacctaga acaagctagg
    61261 tgggattatt tgcctatgaa atgagaaatt atgggagcaa gctcctaaat caactgtttt
    61321 tgatctactt ctttaactac ttgataaaag ttatagaagt aggccaaact tgaaatgatg
    61381 gttacgacta ggaatattga aaatttccat tggacagggt tggttaaaag ttgtggaaag
    61441 gatatgagga gaaagaagag ggctgcgttg aggacaggta tccgttttga ttgtattttc
    61501 tcaagtcctt tattgagcgc aggaagaaag aggagtagga gtagtaaaac tgtatgagaa
    61561 acagctcctg aaataagggc aaagaaaagg aaaatactga taaaaacatg aatgatcagt
    61621 agtctagcta gtgatttcat aaggtacctc ctaatcctgg tcttttttag ctcttgcaat
    61681 acgaagtgag tcgacaatat gtatcatcac tccgaaaaag aaagctccca gtatagtttt
    61741 aaaaatatgt tttgtattta gaagagaact gataaaattt ggattttcac ttgttagggt
    61801 atcaatgagt ggaattataa aaaatatcac tgttccataa atcgaacctg ctttcagacc
    61861 aggataacgt aactgtttct tttctttttt catgagtttg ctcctttttt aaaaatcttc
    61921 ctcccaaaag agactgttga ggtcagtttg gaggctgcgg gcgagattga gacagagttc
    61981 caaggttgga ttgtacttgt cgttttcaat catattgata gtctgtctcg agacaccgat
    62041 atccttggcg agttcgagct gggaaatacc caattccttg cgaaattctt tcacacgatt
    62101 catctggtct cctttctgat ttatgtcgta tatatttgac tatattatag tcttttaaac
    62161 ataaagtgtc aagtattttt gacatatttt ttgaagaaat agtagtctcc ttgtcctatt
    62221 tgtctgacaa gtgcaagctg gtcggatttg tggtaaaata gataagatat gacaaaagaa
    62281 tttcatcatg taacggtctt actccacgaa acgattgata tgcttgacgt aaagcctgaa
    62341 ggtatctacg ttgatgcgac tttgggcgga gcaggacata gcgagtattt attaagtaaa
    62401 ttaagtgaaa aaggccatct ctatgccttt gaccaggatc agaatgccat tgacaatgcg
    62461 caaaaacgct tggcacctta cattgagaag ggaatggtga cctttatcaa ggataacttc
    62521 cgtcatttac aggcacgttt gcgcgaagct ggtgttcagg aaattgatgg aatttgttat
    62581 gacttgggag tgtctagtcc tcaattggac cagcgtgagc gtggtttttc ttataaaaag
    62641 gatgcgccac tggacatgcg gatgaatcag gatgctagtc tgacagccta tgaagtggtt
    62701 aatcattatg actatcatga tttggttcgt attttcttca aatacggtga ggataaattc
    62761 tctaaacaga ttgcgcgtaa gattgagcaa gcgcgtgaag tgaagccgat tgagacaacg
    62821 actgagttag cagagattat caagttggtc aaacctgcca aggaactcaa gaagaagggt
    62881 catcctgcta agcagatttt ccaggctatt cgaattgaag tcaatgatga actgggagcg
    62941 gcagatgagt ccatccagca ggctatggat atgttggctc tggatggtag aatttcagtg
    63001 attacctttc attccttaga agaccgcttg accaagcaat tgttcaagga agcttcaaca
    63061 gttgaagttc caaaaggctt gcctttcatc ccagatgatc tcaagcccaa gatggaattg
    63121 gtgtcccgta agccaatctt gccaagtgcg gaagagttag aagccaataa ccgctcgcac
    63181 tcagccaagt tgcgcgtggt cagaaaaatt cacaagtaag agggaaaaag atggcagaaa
    63241 aaatggaaaa aacaggtcaa atactacaga tgcaacttaa acggttttcg cgtgtggaaa
    63301 aagcttttta cttttccatt gctgtaacca ctcttattgt agccattagt attattttta
    63361 tgcagaccaa gctcttgcaa gtgcagaatg atttgacaaa aatcaatgcg cagatagagg
    63421 aaaagaaaac cgaattggac gatgccaagc aagaggtcaa tgaactatta cgtgcagaac
    63481 gtttgaaaga aattgccaat tcacacgatt tgcaattaaa caatgaaaat attagaatag
    63541 cggagtaaga tatgaagtgg acaaaaagag taatccgtta tgcgaccaaa aatcggaaat
    63601 cgccggctga aaacagacgc agagttggaa aaagtctgag tttattatct gtctttgttt
    63661 ttgccatttt tttagtcaat tttgcggtca ttattgggac aggcactcgc tttggaacag
    63721 atttagcgaa ggaagctaag aaggttcatc aaaccacccg tacagttcct gccaaacgtg
    63781 ggactattta tgaccgaaat ggagtcccga ttgctgagga tgcaacctct tataatgtct
    63841 atgcggtcat tgatgagaac tataagtcag caacgggtaa gattctttac gtagaaaaaa
    63901 cacaatttaa caaggttgca gaggtctttc ataagtatct ggacatggaa gaatcctatg
    63961 taagagagca actctcgcaa cctaatctca agcaagtttc ctttggagca aagggaaatg
    64021 ggattaccta tgccaatatg atgtctatca aaaaagaatt ggaagctgca gaggtcaagg
    64081 ggattgattt tacaaccagt cccaatcgta gttacccaaa cggacaattt gcttctagtt
    64141 ttatcggcct agctcagctc catgaaaatg aagatggaag caagagcttg ctgggaacct
    64201 ctggaatgga gagttccttg aacagtattc ttgcagggac agacggcatt attacctatg
    64261 aaaaggatcg tctgggtaat attgtacccg gaacagaaca agtttcccaa cgaacgatgg
    64321 acggtaagga tgtttataca accatttcca gccccctcca gtcctttatg gaaacccaga
    64381 tggatgcttt tcaagagaag gtaaaaggaa agtacatgac agcgactttg gtcagtgcta
    64441 aaacagggga aattctggca acaacgcaac gaccgacctt tgatgcagat acaaaagaag
    64501 gcattacaga ggactttgtt tggcgtgata tcctttacca aagtaactat gagccaggtt
    64561 ccactatgaa agtgatgatg ttggctgctg ctattgataa taataccttt ccaggaggag
    64621 aagtctttaa tagtagtgag ttaaaaattg cagatgccac gattcgagat tgggacgtta
    64681 atgaaggatt gactggtggc agaatgatga ctttttctca aggttttgca cactcaagta
    64741 acgttgggat gaccctcctt gagcaaaaga tgggagatgc tacctggctt gattatctta
    64801 atcgttttaa atttggtgtt ccgacccgtt tcggtttgac ggatgagtat gctggtcagc
    64861 ttcctgcgga taatattgtc aacattgcgc aaagctcatt tggacaaggg atttcagtga
    64921 cccagacgca aatgattcgt gcctttacag ctattgctaa tgacggtgtc atgctggagc
    64981 ctaaatttat tagtgccatt tatgatccaa atgatcaaac tgctcggaaa tctcaaaaag
    65041 aaattgtggg aaatcctgtt tctaaagatg cagctagtct aactcggact aacatggttt
    65101 tggtagggac ggatccggtt tatggaacca tgtataacca cagcacaggc aagccaactg
    65161 taactgttcc tgggcaaaat gtagccctca agtctggtac ggctcagatt gctgacgaga
    65221 aaaatggtgg ttatctagtc ggggtaaccg actatatttt ctcggctgta tcgatgagtc
    65281 cggctgaaaa tcctgatttt atcttgtatg tgacggtcca acaacctgaa cattattcag
    65341 gtattcagtt gggagaattt gccaatccta tcttggagcg ggcttcagct atgaaagact
    65401 ctctcaatct tcaaacaaca gctaaagctt tggagcaagt aagtcaacaa agtccttatc
    65461 ctatgcctag tgtcaaggat atttcacctg gtgatttagc agaagaattg cgtcgcaatc
    65521 ttgtacaacc catcgttgtg ggaacaggaa cgaagattaa aaacagttct gctgaagaag
    65581 ggaagaatct tgccccgaac cagcaagtcc ttatcttatc tgataaagta gaggaagttc
    65641 cggatatgta tggttggaca aaggagactg ctgagaccct tgctaagtgg ctcaatatag
    65701 aacttgaatt tcaaggttcg ggctctactg tgcagaagca agatgttcgt gctaacacag
    65761 ctatcaagga cattaaaaaa attacattaa ctttaggaga ctaatatgtt tatttccctc
    65821 agtgctggaa ttgtgacatt tttactaact ttagtaggaa ttccggcctt tatccaattt
    65881 tatagaaagg cgcaaattac aggccagcag atgcatgagg atgtcaaaca gcatcaggca
    65941 aaagctggga ctcctacaat gggaggtttg gttttcttga ttacttctgt tttggttgct
    66001 ttctttttcg ccctatttag tagccaattc agcaataatg tgggaatgat tttgttcatc
    66061 ttggtcttgt atggcttggt cggattttta gatgactttc tcaaggtctt tcgtaaaatc
    66121 aatgaggggc ttaatcctaa gcaaaaatta gctcttcagc ttctaggtgg agttatcttc
    66181 tatcttttct atgagcgcgg tggcgatatg ctttctgtct ttggttatca agtgcatcta
    66241 gggattttct atattgtctt cgctcttttc tggctagtcg gtttttcaaa cgcagtaaac
    66301 ttgacagacg gtgttgacgg tttagctagt atttccgttg tgattagttt gtctgcctat
    66361 ggagttattg cctatgtgca aggtcagatg gatattcttc tagtgattct tgccatgatt
    66421 ggtggtttgc tcggtttctt catctttaac cataagcctg ccaaggtctt tatgggtgat
    66481 gtgggaagtt tggccctagg tgggatgctg gcagctatct ctatggctct ccaccaagaa
    66541 tggactctct tgattatcgg aattgtatat gtttttgaaa caacttctgt tatgatgcaa
    66601 gtcagttatt tcaaactgac aggtggtaaa cgtattttcc gtatgacgcc tgtacatcac
    66661 cattttgagc ttgggggatt gtctggtaaa ggaaatcctt ggagcgagtg gaaggttgac
    66721 ttcttctttt ggggagtggg acttctagca agtctcctga ccctcgcaat tttatatttg
    66781 atgtaagaat ggcaccctga tgtttcaggg tgtttttgtg tttaaataca caatgaaaat
    66841 caaagaacaa actagaaagc taactttagg ctgctcaaaa tatagtagat tgaaactaga
    66901 atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactgtcc tgatcgattt
    66961 gtcctgttct tatttcattt taatatacag ttttgaggtt gtagataagg agaagctgat
    67021 gtggtttgaa gaggttttcg aagagtgtaa aaaaatattg gtcagttaaa gtcaaaactc
    67081 ttgacctttt ctgactaatg tagtatatta tgaacgtaag gtaaatgaaa gccttactag
    67141 aacacttatt taaagtaaaa tagaaagagg tgggtctatg tttcatctag aaatcttcag
    67201 aagtaaagat agtctactcc tgcttgaaaa agaaaaacca gaaatagtac gtagggtagc
    67261 gatctagcta gtctaaattt tttaaaacaa tggtcaaaga tagtcaatat cagtaatcat
    67321 aactaagtaa acaaaaagag gtaaagaata tgaataacaa ctttaataat tttaataaca
    67381 tggatgattt atttaaccaa ttgatgggtg gtatgcgagg atacagttct gaaaatcgcc
    67441 gttacttaat taatggacgc gaagtcacac ctgaggaatt tgctcactat cgtacgactg
    67501 gtcaattacc aggaaatgca gaaactgatg tgcaaatgcc acaacaggca tcaggtatga
    67561 aacaagacgg tgtccttgca aaactaggtc gaaacttgac agcagaagcg cgtgagggca
    67621 agttggatcc tgttatcgga cgaaacaagg aaattcaaga aacatctgaa atcctctcac
    67681 gccgcaccaa gaacaatcct gttttggtcg gagatgcagg tgttggtaag acagcagttg
    67741 tcgaaggtct agcgcaagcc attgtgaacg gagatgttcc tgctgctatc aagaacaagg
    67801 aaattatttc tattgatatc tcaggtcttg aggctggtac tcaataccgt ggtagctttg
    67861 aagaaaatgt ccaaaactta gtcaatgaag tgaaagaagc agggaatatt atcctcttct
    67921 ttgatgaaat tcaccaaatt cttggtgctg gtagcactgg tggagacagt ggttctaaag
    67981 ggcttgcgga tattctcaag ccagctctct ctcgtggaga attgacagtg attggggcaa
    68041 caactcaaga cgaataccgt aacaccatct tgaagaatgc tgctcttgct cgtcgtttca
    68101 acgaagtgaa ggtcaatgct ccttcagcag agaatacttt taaaattctt caaggcattc
    68161 gtgacctcta tcaacaacac cacaatgtca tcttgccaga cgaagtcttg aaagcagcgg
    68221 tggattattc tgttcaatac attcctcaac gtagcttgcc agataaggct attgaccttg
    68281 tcgatgtaac ggctgctcac ttggcggctc aacatccagt aacagatgtg catgctgttg
    68341 aacgagaaat cgaaacggaa aaagacaagc aagaaaaagc agttgaagca gaagattttg
    68401 aagcagctct aaactataaa acacgcattg cagaattgga aaagaaaatc gaaaaccaca
    68461 cagaagatat gaaagtgact gcaagtgtca acgatgtggc tgaatctgtg gaacgaatga
    68521 caggtatccc agtatcgcaa atgggagctt cagatatcga acgtttgaaa gatatggctc
    68581 atcgcttgca agacaaggtg attggtcaag ataaggccgt agaagctgta gctcgtgcta
    68641 tccgtcgtaa ccgtgctggt tttgatgaag gaaatcgccc aatcggcagc ttcctctttg
    68701 tagggtctac tggggttggt aagacggagc ttgctaagca attggcactc gatatgtttg
    68761 gaacccagga tgcgattatc cgtttggata tgtctgaata cagtgaccgc acagctgttt
    68821 ctaagctaat tggtacaaca gcaggctatg tgggttatga tgacaatagc aataccttaa
    68881 cagaacgtgt tcgtcgcaat ccatactcta tcattctctt ggatgaaatt gaaaaggctg
    68941 atcctcaagt tattaccctt ctcctccaag ttctagatga tggtcgtttg acagatggtc
    69001 aaggaaatac agtaaacttc aagaacactg tcattattgc gacctcaaat gctggatttg
    69061 gctatgaagc caacttgaca gaagatgcgg ataaaccaga attgatggac cgtttgaaac
    69121 ccttcttccg tccagaattc ctcaaccgct ttaatgcagt catcgagttt tcacacttga
    69181 ctaaggaaga cctttctaag attgtagatt tgatgttggc tgaagttaac caaaccttgg
    69241 ctaagaaaga cattgacttg gtagtcagtc aagcggctaa agattatatc acagaagaag
    69301 gttacgacga agtcatgggg gttcgtcctc tccgtcgcgt ggttgaacaa gaaattcgtg
    69361 ataaggtgac agacttccac ttggatcatt tagatgctaa acatctggaa gcagatatgg
    69421 aagatggcgt tttggttatt cgtgagaaag cctaagacag aattttgaga ataaaaaaga
    69481 aggagccagc tgaaaaaaac tggttccttt tgaggtacga cgggcatgtc gtgtagtaga
    69541 gatgtactat tctagtttca atctactata gtagctcaga aatcggtact taaacgtgct
    69601 atatcaaaac cagtccttga aaaacgtgga ctggtttcgt gtttggatta ttactttgaa
    69661 cgacatgcgt taaaagttag ttgaaccgcc gtatgccgaa cggtacgtac ggtggtgtga
    69721 gaggggctag agattatccc ctactcgatt ttaaatcaca tgacgttcaa aggcatcatc
    69781 tgaaatccct tgttccaaga tgagttttgc ccattcttta gcagagaaga ggctgtggtc
    69841 cttgtagttt ccgcaagatt cgatggttgt ccctgggaca tcttcccaag tagtagtttc
    69901 agcgatttcc ttgagcgaat ccttgataac agctgcgatt ttagcactgg tgtgacgtcc
    69961 ccacataatc atgtggaagc ctgtgcggca accaaatggc gaacagtcaa tcatgccgtc
    70021 aatgcgggta cggatgagtt tggctaagag gtgctcgata gtgtgaaggc cggcggtagg
    70081 gatagagtct tcgtttggtt gcaccaagcg aatatcataa ttggagatga tgtctccttt
    70141 tggtcctgtt tcttccccaa tcaagcgaac atagggtgct ttgacaatgg tgtggtcaag
    70201 ttcaaaactt tcgacaataa cttcttttga catggtaaat cctttcagtt ttcttctctc
    70261 attatatcat aaaggttgct cctgagacag agagaaaacc tctccgaggc tggagaggtt
    70321 gaaatcttta cttacgatat aagcggtcgt attggtagta tgggtcaaag gttacgttga
    70381 tacccagttt acgaaggaca ttcttgtctt catcagtcaa gatgatggtt gagtgggctt
    70441 cgcttccttt gaggttgccg agttcttcca tagcgcgggt agcatcagga ttttctgtag
    70501 ctgtgatagc aagtgcaatc aggatttcat ttgaatgaag gcgtggattg cggctaccga
    70561 gatgatcgat tttaagacct tggattggct taacaacttc cggctcgatt aattttactt
    70621 ctttagcgat gtcagctgat tttttgatgg cgttgatcaa ggcagcggct gtagggccaa
    70681 agagttctga gttcttacca gtgacgattt ccccatttgg caattcaaag gctagggctg
    70741 gtccaccagt ttcttctgct ttttggcgcg caacgacagc aaccttacgg tctgcaggtg
    70801 tgataccgag gtcgttcatg agcaactcaa ttttcttgac ggcagcttcg ccaacttttt
    70861 cagctttgaa gtcaagaact gtttgatagt aacggcggat gatttcttgt ttagaagctt
    70921 cgacagcggc ctcgtcatct gtaatagcga aaccaaccat gttgacaccc atatctgtcg
    70981 gtgaagcgta tggtgatttt ccgagaatac gttccaacat gcgtttgagc actgggaaga
    71041 tttcgatatc acggttgtag ttgacagtgg tttctccata ggtttggaga tggaaggggt
    71101 caatcatgtt gacatcatca aggtcagctg tggcagcttc ataagccaag ttaactggat
    71161 gatgaagggg aagattccaa acagggaagg tttcaaattt agcgtagcca gatttgatgc
    71221 cattgatttg gtcgtggtac atattggaca tacacgttgc caattttcca gaaccaggtc
    71281 caggagcggt tacgacaatc aagttgcgac tggttttgat gtagtcgttt ttgcccatgc
    71341 cttctgggga aatgatgtga tccatatccg tcggatatcc tttgattgga taatgaagat
    71401 atgaatcaat tccgtttttc tcaagttggt tgcggaaggc atctgcagcg ggttggccag
    71461 cgtattgtgt aatgacaacg gaaccaacaa aaatccccaa ttcattgaat ttgtcaatca
    71521 aacgaagaac ttcttggtca taagaaatgc ctaagtcgcc acgtgctttg gaatgttcaa
    71581 tgttgctagc attaatggca atcacaacct caacctgctc tttcaattct tgcaagagct
    71641 tgattttgtt gtcaggttca taaccaggaa ggacacgagc agcgtggaaa tcttctaaca
    71701 ttttaccgcc aaactctaag tagagcttgc cgtcaaattg gttaatacgc tccaaaatat
    71761 ggtcgcgttg taaattcaaa tattgttcag aactaaaagc ttgttttttc atttttttac
    71821 ctctggactc tattataata aaaaattgga agttaggaaa ctacggagct aaaaaagaaa
    71881 ttaaaaagat taagcaaacg cttgcacaaa attttaaaaa gtgctatcat agactataga
    71941 ttattaaaat aatgaggtaa acagatgcaa gaaaaatggt ggcataatgc cgtagtctat
    72001 caagtctatc caaagagttt tatggatagt aatggagatg gagttggtga tttgccaggt
    72061 attaccagta agttggacta tctagccaag ctaggaatca cagcaatttg gctttctccc
    72121 gtttatgaca gccctatgga tgataatggt tacgatattg ctgattatca agcgattgca
    72181 acgatttttg gaaccatgga ggatatggat caactgattg cagaagctaa gaagcgtgat
    72241 atccgtatca tcatggactt ggtggtcaat catacctcgg atgagcatgc ctggtttgta
    72301 gaggcctgtg aaaatcctaa tagccctgag cgagactact atatctggcg cgatgaaccc
    72361 aatgacctag attctatctt tagtgggtct gcttgggaat acgatgaaaa gtcaggtcaa
    72421 tactatctcc actttttcag caagaaacag ccggatctca actgggaaaa tgaaaaactt
    72481 cgccagaaaa tttatgagat gatgaacttc tggattgata aaggtattgg tggtttccgt
    72541 atggatgtta ttgacatgat tggcaaaatt cctgacgaga aggtagtcaa taatggtcct
    72601 atgctccatc cctatctcaa ggaaatgaat caggcgacct ttggagataa ggatctcttg
    72661 acagtagggg agacttgggg agcaacgcca gagattgcca agctctactc tgatccaaag
    72721 gggcaagaat tgtctatggt cttccagttt gaacatatcg gtcttcagta tcaggaaggt
    72781 cagcctaaat ggcactatca aaaagagctg aatatcgcta agttaaaaga aatcttcaac
    72841 aaatggcaga cagagttagg agttgaggac ggctggaatt ccctcttctg gaacaaccat
    72901 gacctccctc gtattgtctc aatatgggga aatgaccaag aataccgcga aaaatctgcc
    72961 aaagcctttg caatcttgct tcatcttatg agaggaactc cttatatcta ccaaggtgag
    73021 gagattggga tgaccaacta tccgtttgaa acactagatc aagtagaaga tattgaatct
    73081 ctcaactatg cgcgtgaggc tcttgaaaaa ggtgttccga ttgaagaaat catggacagt
    73141 atccgtgtta ttggacgtga caatgcccgt acccctatgc aatgggacga gagcaaaaac
    73201 gctggtttct caacaggtca accttggttg gcggttaatc caaattacga gatgatcaac
    73261 gtccaagaag cgctggcaaa tccagattct attttctata cctatcagaa actggtccaa
    73321 attcgcaagg agaatagctg gctagttcga gctgactttg aattgcttga tacggctgat
    73381 aaggtctttg cttatatacg taaggatggc gaccgtcgct tcctagttgt ggctaacttg
    73441 tccaatgaag agcaagactt gacagtagaa ggaaaagtca aatctgtctt gattgaaaac
    73501 accctagctc aagaagtctt tgaaaaacaa atcttagttc catgggatgc tttctgtgtg
    73561 gaaatgactg actagaatga gcaaacctca agtttttgaa gcttgaggtt ttttactata
    73621 gtggattgaa actagaatag tgcacctctg cttctaaaac attgttagaa atcgatttga
    73681 ctgtcccgat cgatttgtcc tattcttatt tcattttgct atacttgtag ttgaggagaa
    73741 ttaagctcct cactagtaaa ctcttcataa tcctttttat ttttatgaag atattgtttg
    73801 aaagatgtga gtttccacgg atgggtttgt ggagggatat acttgcgtct ttcttttttt
    73861 gttctggttc ttgttcaaag tttttcgaat agagttcatg atttagtagc tcctttgtgt
    73921 gatagatttt gtcagcgata ttgaggtaga tgtctccgtc aaatgctttt ataactaatg
    73981 ctttcgtctt tctgatgaaa tagacttctt ttccttgttc ggtagggata tagtaacgat
    74041 tttggaatcg gatatggtgt ccactatcga cgactctctc cgccagtcta gccagaatga
    74101 gattcctttc agaggggtta ggaacctcct cgaagacaga gagttttgtc ttgtttccaa
    74161 actgttcatt aaaggtttga atgtaggaaa gcaggaaggt attggcttct tccaaggtat
    74221 gaatattgtt ttgttccagt tcgataggca ggcgagattg tagtgtctga ttgagtcttt
    74281 cgactctccc tttagcttga gggatagagg ttgtctcaag gagaatccct agttggtgac
    74341 aggcgtatcc aaactgtgta tgggtatcgt cctccatttt cttagagttg gaggcttgat
    74401 aggtaaagac cgttctctta tctgttttaa tttgaagggg aatgccgtga ttggctaaga
    74461 tttgttcgag gacatgatag taagcattca agttctcttg tttatcaaaa taagcgccta
    74521 ggatattgcc agaagcatca tcaatggcta agtgtaagtt agatgtttgg gctccaaact
    74581 aggcatgagg gctggcatcc atttgaatga gttcaccagc aaatttcttt ctgggtctac
    74641 taggatgtac ttttttaggg tcttcaagga agttttcagc cgtcggtaag attggattgt
    74701 ctaggggttg attcaggttc agtttagctt gttttcttac tctcttcttt gtctttctat
    74761 gggacttagg cgacaggata tttttcttat agagtatttt tctaacagta gtatcagaga
    74821 gctgaattcc ttcttcttca gctagcaatt cacagaaatg aaggacattt ggtttatatg
    74881 tttcatagga gaggtatttt tttaggagac gttctttgat ttcatcaggg attgcatgtt
    74941 ttggttttcg atttctgttt ccgtgtctga aggcttcttt tcctttcagt tgataggcta
    75001 gtagcagacg attgatttgt ctttcagaaa gattgagctc gacacaggct cgtttctttg
    75061 ttttctttcc ttgggctata gcttttatca caagatattt tttcgtttca ttcatattca
    75121 gttggatcct tttcatatga ctattctacc aaatgggaca ttttcacgtt cgatttacta
    75181 aagacattat cacattcgaa ttacacaaga tgcagatagt gaaaataaag gtgtagacat
    75241 taccgtaaaa aagtgatata attgtatgat gttcaatgta taggtgttaa tcatgagtag
    75301 acgttttaaa aaatcaggtt cacagaaagt gaagcgaagt gttaatatcg ttttgctgac
    75361 tatttattta ttgttagttt gttttttatt gttcttaatc tttaagtaca atatccttgc
    75421 ttttagatat cttaatctag tggtaactgc gttagtccta ctagttgcct tagtagggct
    75481 actcttgatt atctataaaa aagctgaaaa gtttactatt tttctgttgg tgttctctat
    75541 ccttgtcagc tctgtgtcgc tttttgcagt acagcagttt gttggactga ccaatcgttt
    75601 aaatgcgact tcgaattact cagaatattc aatcagtgtc gctgttttag cagatagtga
    75661 gatcgaaaat gttacgcaac tgacgagtgt gacagcaccg actgggactg ataatgaaaa
    75721 tattcaaaaa ctactagctg atatcaagtc aagtcagaat accgatttga cggtcaacca
    75781 gagttcgtct tacttggcag cttacaagag tttgattgca ggggagacta aggccattgt
    75841 cctaaatagt gtctttgaaa atatcatcga gtcagagtat ccagactacg catcgaagat
    75901 aaaaaagatt tataccaagg gattcactaa aaaagtagaa gctcctaaga cgtctaagaa
    75961 tcagtctttc aatatctatg ttagtggaat tgacacctat ggtcctatta gttcggtgtc
    76021 gcgatcagat gtcaatatcc tgatgactgt caatcgagat accaagaaaa tcctcttgac
    76081 cacaacgcca cgtgatgcct atgtaccaat cgcagatggt ggaaataatc aaaaagataa
    76141 attaacccat gcgggcattt atggagttga ttcgtccatt cataccctag agaaccttta
    76201 tggtgtagat attaattatt atgttcgttt gaattttacc tcatttttga aattgattga
    76261 cttattggga ggggtagatg ttcataatga tcaagagttt tcagctctac aataggaagt
    76321 tccttttccc agtagggaat gtccatctag actctgagca ggctctaggt tttgtacgtg
    76381 aacgctactc actagccgat ggagaccgtg accgtggtcg caaccaacaa aaggtgattg
    76441 tggctattct tcaaaaatta acgtcaaccg aagcactgaa aaattatagt acgatcatta
    76501 atagcttgca agattctatc caaacaaata tgccacttga gactatgata aatttggtca
    76561 atgctcagtt agaaagtggt ggaacttaca aagtgaattc tcaagattta aaagggacag
    76621 atcggatgga tcttccttct tatgcaatgc ctgacagtaa cctctatgtg atggaaatag
    76681 atgatagtag tttagctgta gttaaagcag ctatacagga tgtgatggag ggtagatgaa
    76741 atgatagaca tccattcgca tatcgttttt gatgtagatg acggtcccaa gtcaatagag
    76801 gaaagcaagg cacttttaag agaagcttat aatcaaggag ttcgaatgat tgtgtctact
    76861 tcgcatcgtc gaaaagggat gtttgaaact ccggaagaga agatagcaga aaactttctt
    76921 caggttcggg aaattgcaaa agaagtagca gatgatttag tcatagctta tggcgcagag
    76981 atatactata ctctggatgc tctagaaaag ctagaaaaaa aagaaattcc tacccttaat
    77041 gatagtcgtt atgctttgat tgagtttagc atgcatactt cctatcgtga gattcatgcg
    77101 ggattgagca atattttgat gttgggaatc acgccagtaa ttgctcatat tgaacgttat
    77161 gatgccttag agaataacga aaaacgtgtt cgtgaactga ttgatatggg gtgctatact
    77221 cagataaata gttatcatgt tttaaaacct aagttctttg gtgaaaaata taaattcatg
    77281 aaaaagagag ctcggtattt tttggaacgt gatttagttc atgtagttgc aagtgacatg
    77341 cacaatttag acagtagacc tccatatatg caacaggcat atgatatcat tgctaagaaa
    77401 tatggagcga aaaaagcgaa agaatttttt gtagataatc ccagaaaaat tataatggat
    77461 caattaattt aggagaaaat atgaaggaac aaaacacttt ggaaatcgat gtattgcaac
    77521 tattcagggc tttatggaaa agaaagttgg tcattttatt agtggcaatt ataacttctt
    77581 cagttgcttt tgcctacagt acttttgtta tcaaacctga gtttactagt acgactcgga
    77641 tttatgtagt taaccgtaat caggaagaga agtctggttt aaccaatcaa gacttgcagg
    77701 caggatcata cttggttaaa gactatcgtg aaattatcct atcgcaggat gttttggagg
    77761 aagttatttc tgatttgaaa ctagatttga cgccaaaagg tttggctaat aaaattaaag
    77821 taacagtacc agttgatacc cgtattgtct ctgtctcagt taatgatcga gttcctgaag
    77881 aggcaagccg tatcgctaac tctttgagag aagtagctgc tcaaaaaatt atcagtatta
    77941 ctcgtgtttc tgatgtgaca acactggagg aggcaagacc ggcgatatca ccatcttcgc
    78001 caaatattaa acgcaataca ctaattggtt ttttggcagg ggggattgga actagtgtta
    78061 tagttcttct tcttgaactt ttggacactc atgtgaaacg tccggaagat atcgaagata
    78121 cactgcagat gacacttttg ggagttgtac caaacttgag taagttgaaa taggagagag
    78181 gaatgccgac attagaaata gcacaaaaaa aactggagtt cattaagaag gcagaagaat
    78241 attacaatgc cttgtgtaca aatatacagt tgagcggaga taaactaaaa gtaatttccg
    78301 ttacttctgt taaccctggg gaaggaaaaa caactacttc cgtaaatata gcaaggtcgt
    78361 ttgcgcgtac aggctataaa actcttttga tcgatggcga tactcgaaat tcagttatat
    78421 caggagtttt taaatcgcgt gaaaaaatta cagggctaac agaattttta tctgggacag
    78481 ctgatttatc tcacggttta tgtgatacaa atattgaaaa tttatttgta attcaatcgg
    78541 gatctgtatc accaaaccct acagccttgt tacaaagtaa aaattttaat gatatgattg
    78601 aaacattgcg taaatatttt gattatatca ttgttgatac agcacctatt ggaattgtta
    78661 ttgatgcggc aattatcact caaaagtgtg atgcgtccat cttggtaaca gcaataggtg
    78721 aggtgaataa acgtgatgtc caaaaagcga aacaacaatt agaacaaaca gagaaactgt
    78781 tcctaggagt tgttttaaat aaattggata tctcggttga taagtatgga gtttacggtt
    78841 cctatggaaa ttatggtaaa aaataactta ggaaagattt tatggatgaa aaaggattga
    78901 aaatttttct agcagtaata cagagtatta ttgtcatttt attggtttat tttcttagct
    78961 ttgttagaga gacagaactt gaacgttctt cgatggttat actatacctt ctccactttt
    79021 ttgtattcta ttttagttcc tatggtaaca atttttttaa aagagggtac ctagttgagt
    79081 ttaatagtac tataagatat atttttttct ttgcaatagc tataagtgta ttaaactttt
    79141 ttatagcgga acggtttagt atctctagaa gaggaatggt atacttctta actttagaag
    79201 gaatatcctt atacttgtta aatttcttag taaagaaata ttggaagcat gtgtttttta
    79261 atctaaaaaa tagcaagaaa attttactgt taacagtaac gaaaaatatg gaaaaagttc
    79321 ttgataaatt gctagaatct gatgaacttt catggaaatt ggtagcagta agtgttttgg
    79381 ataaatctga ttttcaacat gataaaatac ctgtaattga aaaggaaaaa attattgaat
    79441 ttgcaacgca tgaagttgtg gatgaggtgt ttgtcaatct tccaggagag agctacgata
    79501 ttggagaaat tatctctagg tttgagacaa tggggataga tgtaactgta aatcttaaag
    79561 catttgataa gaatttgggt cgcaataaac aaattcatga gatggtagga ttgaatgtag
    79621 tcactttctc tacaaatttt tataaaacta gtcatgtgat ttcaaagaga attctcgata
    79681 tttgtggtgc cactattggc cttattcttt ttgctatagc tagtctagtt ttagttccat
    79741 tgattcgtaa agatggcgga tcagctattt ttgctcaaac tcgtataggg aaaaatggtc
    79801 gacattttac cttttataaa ttccgttcga tgcgaatcga tgctgaagct atcaaagaac
    79861 agttgatgga tcaaaatacg atgcaaggtg gtatgtttaa gatagacaat gatcctcgtg
    79921 ttacaaaaat tggtcgcttt attcgtaaaa ctagtttaga tgaattgccg cagttttgga
    79981 atgtctttat aggagatatg agtttggtgg gaacacgtcc acctacagta gacgagtatg
    80041 atcagtatac tccagaacag aaacgtcgac tcagctttaa acctggtatt acaggtttat
    80101 ggcaggttag cggccgtagt aaaataaccg attttgacga tgttgtaaaa ttagatgtgg
    80161 cttatattga taattggaca atctggaaag atattgaaat tttgcttaaa actgttaaag
    80221 ttgtatttat gagagatgga gcgaagtaat tcatatgaat gagagaatgc aaatattagg
    80281 agttactata gatccattaa cgatgaaaga aacagtggat gctgttgaac agtatgtttt
    80341 aaagaagcat cctttgcact tgatgggagt taacgcagat aaaattaatc agtgccatga
    80401 agatgagaaa atcaaaaaaa tcgttaatga atcaggaatc attaatgcgg atggagcatc
    80461 agttgttctt gcaagtaagt ttttaggaac gcctgttcct gaacgagtag cgggtattga
    80521 tttgatgcaa tgtcttttag agttgtcaaa taaaaaagga tattcagttt acttttttgg
    80581 agctaaagaa gaaattttac aagatatgct aaaagttttt aaacaaagat atcctaatct
    80641 taatgttgtt ggctatagaa acggatattt ttcacctgaa gatgaaaaaa agattcagga
    80701 agatattaaa ggaggaaaac cagattttgt atttgtaggg attacttctc ctaaaaagga
    80761 gtatatcatc caatcattca tggataatgg gattaatgct gtctttatgg gtgttggtgg
    80821 cagttttgat gtattatcag gacacattaa acgagctcca ttatggatgc aaaaattgaa
    80881 tctggaatgg ttgttccgtg tagttaatga acccaaacgc ctttttaagc gttattttat
    80941 agggaatgtt acttttatta agcgagtttt agatgaaaag cgaaaatcaa aaaaataata
    81001 ttctacatat ttctcgtaca atggatattg gtggtgcaga acgtattgta tatcaattga
    81061 gttctgattt gaaagatgaa tttgatagtg tccatgtcgc ttcaacaggt ggactatggg
    81121 agagcaaact tttatcaaaa ggagttcaac atcataaaat tttagatgtt gacagtaaga
    81181 atcccctaac tatggtaaag atcctttcaa gtttttatag aattatcaaa aataatgata
    81241 ttaccattgt tcacactcat catcggatgg ctgcctttta tattcgcttg ttgaaaatca
    81301 ttaatcctaa gttgatacat gtttacactg cacataatgt atttaaaaat aaattaccat
    81361 tatacaaatt ttccttaaaa aaagcgtatt gtgtagctgt aagtaaagcg gttaatgata
    81421 atttgaaaca tgatgtagga atcagtgata gtacagtaat ctataatggg gttgtttttg
    81481 aaaaaactga taagctagtt ggtgaaattg taaactataa cggaatcaaa ttggggtgta
    81541 ttgctaggtt atcagagcaa aaaggattga cctacctaat tgatgcgatg tctttagtga
    81601 aacacaggaa tattacgttg ttcattgtcg gagatggaga gcttcgagat gagcttgaaa
    81661 acaaggtgaa acaattaaat cttcaaaaat cagtgatatt tttaggttat cgaaaagata
    81721 ttgttgaatg tatcaatagt tttgattact tagtgtcatc atctttatat gaggggtttg
    81781 gtctaaatgc aattgaagca tttatgaagg ggaaaacgat ggtttcatca gatatcccag
    81841 gaataaatga agtagtcaat aacaaaaatg ggattcttgt tcctgttaag gatgatgttg
    81901 ccttagcgag agcgattgaa aagcttgcga ctgacaaaaa acttagagaa aaactcgctt
    81961 atcaagcaaa aaaagattat gagacaaaat ttaattattc cttgtttttg gataattatc
    82021 ggagacttta tagaaaatta atgggagaat caaaatgaaa aaagtcatgt tggtttttgg
    82081 gacacgtcca gaagctatta aaatgtgtcc tttagtaaat gaactgaaac aaaatgattc
    82141 aatcaagaca cttgtgtgtg taactggtca acataaggaa atgttagagc aggttttaga
    82201 ggtgttcaag gttgtcccag attatgattt gggtatcatg aaagcaaatc agaccctatt
    82261 taccattacg actagtattt tggataaaat tcaggcggtt ctagagaaag aaaaaccaga
    82321 tcttgttctt gttcatggag atactacaac aacatttgcg acagctttag cagcatttta
    82381 tatgggaatc aaagtgggac acgtcgaagc aggtctcaga acctataatc tccaaagtcc
    82441 atttccagag gaattcaatc gccagacaac ttctatcatt gccgactatc attttgcgcc
    82501 gactgaagtt gcgaaggaaa atctactaag agaggacaga aaaaatatct ttgtgacggg
    82561 aaatacagtc attgatgctt tgaagacgac tgttcaagat aactaccatc acccaatttt
    82621 agaatgggca aagggtagta agttaatcat gctaacggct catcgacgag aaaatcttgg
    82681 gaaaccgatg gaaaacatgt ttaatgctgt taatcgaatt ttaaacgaat tcgaagatgt
    82741 aaaagttgta tatcccatcc ataaaaatcc taaggttcga gaattagcaa gtaaagtttt
    82801 tggcgataat gaacgaatga aaatcattga accgttagag gttattgatt ttcataattt
    82861 tatgaatcaa agctatatga ttttgacaga ttctggaggt gtacaggaag aagcaccttc
    82921 tttaggaaaa cctgtcttgg tgatgcgtga tacaacagag cgtcctgagg gggtcgttgc
    82981 tggcacgttg aagttggtag gaacagaaga agagaatatc tatagcaact tcaaacttct
    83041 tcttgaagat gaaacagaat ataacaagat gagcaaagct agcaaccctt atggagatgg
    83101 aatgacttgt aaacggattg ttgatattat tctggaagta taaaaataat tcaattatgg
    83161 atgaggaaag aataaattag attgatgata aaaagaaaaa tatatataat ctgtgtatta
    83221 ctgtcgtatt ttttacctag tttttttaca gttggatcaa tggcgacttt atttgctttg
    83281 ctttctactt tagttaagct atttgtaact tttgattata ttcccaaagt tcttttacag
    83341 cataaagtaa cactgattga tattctagtt gttctatttt tattttttca agcatttgca
    83401 gcttttcaaa gccaaacgtt gtaccttaac tatgttggag gtcagttttt ccttttagga
    83461 ctatattcat ttttgaaaca ctttttattg ttggattgta aaacaacaat aaaaagcctc
    83521 tttttaacct ttatcttgtt tttgtgtgtt caagtaataa ctcaattgct ttttccggtc
    83581 ggtttcgatt ctttgcaccc tacaggagat aatagacttt actttttagg aagaaagaac
    83641 atagctaccc cgtatatcat tgttggatta ggtggttttt acttattaaa taaaaaaatg
    83701 aatgagttta tatcactaaa ggagattatt tttttaggat tgttcggaat tttatcattt
    83761 ttaacacaat ccagtacagc gattatatgt tatgggcttt ttattttcat acgtctattg
    83821 ggattaaagg aaaatatagg aaaactatat tctttagtca gtatggctgt atatgtttgt
    83881 ttttctcttt ctataatctt ttcacaaagt acaattttat caacttttac agcgattttt
    83941 tccaaaaatg caactttttc aggacgtatc aatatttggc aattagcgat tcgtattttt
    84001 gaagagaatt tttggtttgg acgaggcctc aacgttaatt ttaatgcttg gacaaatggg
    84061 attatcgtta attcagcaca caatacctta ttagatatac ttgctcgaac tggtatattt
    84121 ccaggtgttt tatttatagt gcttcttatg aatctgtttc ttggaaaata tagaatagaa
    84181 tcaaaaacac tgttggctat gttagcctca tttatgattt atattacaat ggagacttca
    84241 tctatgagca tattgctgct aattattgtt ctttgtgtat attggccacg tggagaggag
    84301 aagttgcatg agcaagttac ttaataccat ctattatcga ctatttagat tatataaaaa
    84361 aatttatcta caagttttta gtaaaaaaac ggttgacatc ttgggtgaaa aggctaattt
    84421 agaaaatgaa ctaattgttt cgtttacaac aattccaagt cgtcttaatt atttaccaag
    84481 tatgattaaa agtattttca atcagacaat tatccctaac cgttttatta tgtatgttta
    84541 taaagatgaa tttgaaggca ttaatttgga gagtattcta gaacttgaaa tcaaaaatgg
    84601 tttggaaatt gtctatgtgg atgagaatct tcgttcacac aaaaaatatt tttatgctat
    84661 gaaggataat ccgaacagta tagttgttct agttgatgac gatatcattt atcctcgaaa
    84721 tactataaag aaactgattg catcctatag aatttatcca cagtgtgttt cagcaatgcg
    84781 atgtcatcga attaagttgt tttctgatgg aagtttatat ccatataatc aatgggaata
    84841 tgaaatatca ggagctacta taccatcgta ttttaatttt tttactagcg gaggtgggac
    84901 attatttcct ccatgtactc gcaatgagga tctttttaat aaaaaaaata tcagggaact
    84961 cagttttcta gctgatgatg tatggttgaa ttttttggta gtcaaaaatg gaattaaaac
    85021 agtaaaagca acacgatata aagggacacc attaactata gatgacaatc ctgaggagag
    85081 tttggtatat ttgaataccg tatatgataa caacaatgat aaatgtatta gaaatatggt
    85141 tgaatattat caaattaatt ttacagagga taaataatga atgttgaaaa aaatttgaaa
    85201 cgtggaattt tctatactgc aatagggaag tattcgaatg tagttattca attacttgta
    85261 acagcaattc ttagccggat tctctcacct gtagaatatg gtatcgtagc agtagtaaat
    85321 gtatttcttt tattttttca aatgttagca gattcaggaa taggacctgc tattgttcaa
    85381 aataaagaac tgaataagtc agatttaatc agcattttct cattaacgat ttatagtgga
    85441 atcgtacttt cttgtatttt tgctttacta ggatatccta tgggggtgtt atacggcgaa
    85501 actatttatg ttagtttatt tccgctttta ggtctttgtg ttttatttta taccattaca
    85561 attgtaccgc aagctattct aatgaaaacg atgaacttta aaatagttaa ttttctaact
    85621 attttttcta acattgctag tggcttggtg ggcgttattc tagcggtatc acattttggt
    85681 gtctattctc taattttttc taatattgta aaagcaatgg ttttatttat cgctttatat
    85741 tctaaagcaa agataaattt ctattggaaa gtttcaaaaa gttctattgg taaaattttt
    85801 gagttttcaa aattccaatt tttgtttaat ttttggaatt attttgctag aaatttagat
    85861 aatttattaa ttggacgatt gattagcccg tcagatcttg gttattatga taaggcttat
    85921 caattatctt tgtaccctaa tcaaatttta tcacaggttg tgacaccagc attgcatcct
    85981 attatgtcca attttcaaga taatatcagt aagataggcg aagtttatct tcagatttct
    86041 agaatattcg taatagttgg catacctatt tcggcttatt tatactttaa tgctcaatat
    86101 gttgttacat ttatgtttgg agataattgg tcccaaagcg ttccggtgtt tcaaatatta
    86161 gcagcaacaa tttggttaca aatggctaat agccctactg gtgcatttta ccaagcaact
    86221 aatcagacaa agttattgtt tagaattggc ttattgacat cttttattaa tattttagct
    86281 attgttattg gtgtaatgtt gcaaagcatc caatgtgttg cgtacatgct tttgatttct
    86341 ttcagtctta
//
LOCUS       DAVKFH010000002        74799 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKFH010000002 DAVKFH010000000
VERSION     DAVKFH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.002"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..586)
                     /locus_tag="VW006_000078"
     CDS             complement(<1..586)
                     /locus_tag="VW006_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEU9379011.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNI"
     gene            complement(745..2172)
                     /locus_tag="VW006_000079"
     CDS             complement(745..2172)
                     /locus_tag="VW006_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9379012.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            2337..3209
                     /gene="hslO"
                     /locus_tag="VW006_000080"
     CDS             2337..3209
                     /gene="hslO"
                     /locus_tag="VW006_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9379013.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALAILPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            3196..4176
                     /gene="dusB"
                     /locus_tag="VW006_000081"
     CDS             3196..4176
                     /gene="dusB"
                     /locus_tag="VW006_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9379014.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(4406..6325)
                     /gene="pspC"
                     /locus_tag="VW006_000082"
     CDS             complement(4406..6325)
                     /gene="pspC"
                     /locus_tag="VW006_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9379015.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSEN
                     NPTVTSSGQDISKKYADEVKSHLEKILSEIQTNLDRSKHIKTVNLINKLQDIKRTYLY
                     ELNVLEDKSKAELPSKIKAELDAAFEQFKKDTLPTEPGKKVAEAKKKVEEAEKKAKAQ
                     KEEDYRNYPTITYKTLELEIAESDVKVKEAELELVKKEADESRNEGTINQAKAKVESE
                     QAEATRLKKIKTDREKAEEEAKRRADAKEQDESKRRKSRVKRGDLGEPATPDKKENDA
                     KSSDSSVGEETLPSPSLKPGKKVAEAEKKVEEAEKKAKDQKEEDHRNYPTITYKTLEL
                     EIAESDVEVKKAELELVKEEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAE
                     EEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEED
                     YARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQYNG
                     SWYYLNANGDMATGWFQYNGSWYYLNANGSMATGWLQYNGSWYYLNANGSMATGWLQY
                     NGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNT
                     TVDSYRVNANGEWVN"
     gene            complement(6404..6931)
                     /locus_tag="VW006_000083"
     CDS             complement(6404..6931)
                     /locus_tag="VW006_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9379016.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            complement(7014..8354)
                     /gene="hk06"
                     /locus_tag="VW006_000084"
     CDS             complement(7014..8354)
                     /gene="hk06"
                     /locus_tag="VW006_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9379017.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSRLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(8351..9004)
                     /gene="rr06"
                     /locus_tag="VW006_000085"
     CDS             complement(8351..9004)
                     /gene="rr06"
                     /locus_tag="VW006_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9379018.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(9325..11757)
                     /locus_tag="VW006_000086"
     CDS             complement(9325..11757)
                     /locus_tag="VW006_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9379019.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(11759..12217)
                     /locus_tag="VW006_000087"
     CDS             complement(11759..12217)
                     /locus_tag="VW006_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9379020.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            complement(12336..13064)
                     /locus_tag="VW006_000088"
     CDS             complement(12336..13064)
                     /locus_tag="VW006_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379021.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(13064..14071)
                     /locus_tag="VW006_000089"
     CDS             complement(13064..14071)
                     /locus_tag="VW006_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9379022.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(14109..14867)
                     /locus_tag="VW006_000090"
     CDS             complement(14109..14867)
                     /locus_tag="VW006_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379023.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            complement(14830..15120)
                     /locus_tag="VW006_000091"
     CDS             complement(14830..15120)
                     /locus_tag="VW006_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9379024.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(15191..15293)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15376..16494)
                     /gene="cbpD"
                     /locus_tag="VW006_000092"
     CDS             complement(15376..16494)
                     /gene="cbpD"
                     /locus_tag="VW006_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9379025.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTV
                     GWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGLWYYLGSSGAM
                     KTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(16583..16849)
                     /locus_tag="VW006_000093"
     CDS             complement(16583..16849)
                     /locus_tag="VW006_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379026.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(16851..18203)
                     /gene="dnaB"
                     /locus_tag="VW006_000094"
     CDS             complement(16851..18203)
                     /gene="dnaB"
                     /locus_tag="VW006_000094"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9379027.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILVDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(18247..18699)
                     /gene="rplI"
                     /locus_tag="VW006_000095"
     CDS             complement(18247..18699)
                     /gene="rplI"
                     /locus_tag="VW006_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9379028.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(18696..20669)
                     /locus_tag="VW006_000096"
     CDS             complement(18696..20669)
                     /locus_tag="VW006_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9379029.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(20805..21353)
                     /gene="raiA"
                     /locus_tag="VW006_000097"
     CDS             complement(20805..21353)
                     /gene="raiA"
                     /locus_tag="VW006_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9379030.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(21433..22095)
                     /locus_tag="VW006_000098"
                     /pseudo
     CDS             complement(21433..22095)
                     /locus_tag="VW006_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            complement(22092..23390)
                     /locus_tag="VW006_000099"
     CDS             complement(22092..23390)
                     /locus_tag="VW006_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9379031.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSNLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            23446..24081
                     /locus_tag="VW006_000100"
     CDS             23446..24081
                     /locus_tag="VW006_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9379032.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDNGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            24366..25286
                     /gene="cysK"
                     /locus_tag="VW006_000101"
     CDS             24366..25286
                     /gene="cysK"
                     /locus_tag="VW006_000101"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9379033.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYNGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(25483..25881)
                     /locus_tag="VW006_000102"
     CDS             complement(25483..25881)
                     /locus_tag="VW006_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9379034.1"
                     /translation="MKAPIPKAPLAHSFGSASIIAHTIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            25872..26159
                     /locus_tag="VW006_000103"
                     /pseudo
     CDS             25872..26159
                     /locus_tag="VW006_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26314..26637)
                     /gene="tnpB"
                     /locus_tag="VW006_000104"
                     /pseudo
     CDS             complement(26314..26637)
                     /gene="tnpB"
                     /locus_tag="VW006_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(26883..27923)
                     /locus_tag="VW006_000105"
     CDS             complement(26883..27923)
                     /locus_tag="VW006_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9379035.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(28002..28781)
                     /gene="rpsB"
                     /locus_tag="VW006_000106"
     CDS             complement(28002..28781)
                     /gene="rpsB"
                     /locus_tag="VW006_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9379036.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            complement(29005..30183)
                     /locus_tag="VW006_000107"
     CDS             complement(29005..30183)
                     /locus_tag="VW006_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9379037.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            complement(30277..30771)
                     /gene="mreD"
                     /locus_tag="VW006_000108"
     CDS             complement(30277..30771)
                     /gene="mreD"
                     /locus_tag="VW006_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9379038.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            complement(30771..31589)
                     /gene="mreC"
                     /locus_tag="VW006_000109"
     CDS             complement(30771..31589)
                     /gene="mreC"
                     /locus_tag="VW006_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9379039.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            complement(31648..32442)
                     /locus_tag="VW006_000110"
     CDS             complement(31648..32442)
                     /locus_tag="VW006_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9379040.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            complement(32435..33274)
                     /locus_tag="VW006_000111"
     CDS             complement(32435..33274)
                     /locus_tag="VW006_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9379041.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            complement(33259..34086)
                     /locus_tag="VW006_000112"
     CDS             complement(33259..34086)
                     /locus_tag="VW006_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9379042.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            complement(34083..34628)
                     /gene="pgsA"
                     /locus_tag="VW006_000113"
     CDS             complement(34083..34628)
                     /gene="pgsA"
                     /locus_tag="VW006_000113"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9379043.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            complement(34639..35460)
                     /locus_tag="VW006_000114"
     CDS             complement(34639..35460)
                     /locus_tag="VW006_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9379044.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFIFFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            complement(35502..36785)
                     /locus_tag="VW006_000115"
     CDS             complement(35502..36785)
                     /locus_tag="VW006_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9379045.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            complement(36782..38032)
                     /locus_tag="VW006_000116"
     CDS             complement(36782..38032)
                     /locus_tag="VW006_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9379046.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            38191..38559
                     /gene="yaaA"
                     /locus_tag="VW006_000117"
     CDS             38191..38559
                     /gene="yaaA"
                     /locus_tag="VW006_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9379047.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            38562..39659
                     /gene="recF"
                     /locus_tag="VW006_000118"
     CDS             38562..39659
                     /gene="recF"
                     /locus_tag="VW006_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9379048.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(39710..41188)
                     /gene="guaB"
                     /locus_tag="VW006_000119"
     CDS             complement(39710..41188)
                     /gene="guaB"
                     /locus_tag="VW006_000119"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9379049.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(41340..42365)
                     /gene="trpS"
                     /locus_tag="VW006_000120"
     CDS             complement(41340..42365)
                     /gene="trpS"
                     /locus_tag="VW006_000120"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9379050.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            42571..44193
                     /locus_tag="VW006_000121"
     CDS             42571..44193
                     /locus_tag="VW006_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9379051.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            44255..46807
                     /locus_tag="VW006_000122"
     CDS             44255..46807
                     /locus_tag="VW006_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9379052.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(46908..47222)
                     /locus_tag="VW006_000123"
     CDS             complement(46908..47222)
                     /locus_tag="VW006_000123"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9379053.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(47250..47588)
                     /locus_tag="VW006_000124"
     CDS             complement(47250..47588)
                     /locus_tag="VW006_000124"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379054.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            complement(47673..48440)
                     /locus_tag="VW006_000125"
     CDS             complement(47673..48440)
                     /locus_tag="VW006_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9379055.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDSYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            48619..49161
                     /locus_tag="VW006_000126"
     CDS             48619..49161
                     /locus_tag="VW006_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9379056.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDVIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(49202..49275)
                     /locus_tag="VW006_000127"
     tRNA            complement(49202..49275)
                     /locus_tag="VW006_000127"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49239..49241),aa:Asn,seq:gtt)
     gene            complement(49281..49352)
                     /locus_tag="VW006_000128"
     tRNA            complement(49281..49352)
                     /locus_tag="VW006_000128"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49317..49319),aa:Glu,seq:ttc)
     gene            complement(49404..50156)
                     /locus_tag="VW006_000129"
     CDS             complement(49404..50156)
                     /locus_tag="VW006_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9379057.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(50153..51478)
                     /locus_tag="VW006_000130"
     CDS             complement(50153..51478)
                     /locus_tag="VW006_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9379058.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYIFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     FKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(51499..51624)
                     /gene="comC"
                     /locus_tag="VW006_000131"
     CDS             complement(51499..51624)
                     /gene="comC"
                     /locus_tag="VW006_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9379059.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIKGGEMRISRIILDFLFLRKK"
     gene            complement(51789..51862)
                     /locus_tag="VW006_000132"
     tRNA            complement(51789..51862)
                     /locus_tag="VW006_000132"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51826..51828),aa:Arg,seq:tct)
     gene            complement(51906..52385)
                     /gene="rlmH"
                     /locus_tag="VW006_000133"
     CDS             complement(51906..52385)
                     /gene="rlmH"
                     /locus_tag="VW006_000133"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9379060.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            52568..53749
                     /locus_tag="VW006_000134"
     CDS             52568..53749
                     /locus_tag="VW006_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9379061.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            53807..54565
                     /locus_tag="VW006_000135"
     CDS             53807..54565
                     /locus_tag="VW006_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9379062.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            54778..56139
                     /gene="dnaA"
                     /locus_tag="VW006_000136"
     CDS             54778..56139
                     /gene="dnaA"
                     /locus_tag="VW006_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9379063.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            56298..57434
                     /gene="dnaN"
                     /locus_tag="VW006_000137"
     CDS             56298..57434
                     /gene="dnaN"
                     /locus_tag="VW006_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9379064.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            57499..57693
                     /locus_tag="VW006_000138"
     CDS             57499..57693
                     /locus_tag="VW006_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9379065.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEVTRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            57777..58892
                     /gene="ychF"
                     /locus_tag="VW006_000139"
     CDS             57777..58892
                     /gene="ychF"
                     /locus_tag="VW006_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9379066.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            58964..59533
                     /locus_tag="VW006_000140"
     CDS             58964..59533
                     /locus_tag="VW006_000140"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9379067.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            59534..63043
                     /gene="mfd"
                     /locus_tag="VW006_000141"
     CDS             59534..63043
                     /gene="mfd"
                     /locus_tag="VW006_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9379068.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            63101..63367
                     /locus_tag="VW006_000142"
     CDS             63101..63367
                     /locus_tag="VW006_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9379069.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            63360..63728
                     /locus_tag="VW006_000143"
     CDS             63360..63728
                     /locus_tag="VW006_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9379070.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            63848..65116
                     /locus_tag="VW006_000144"
     CDS             63848..65116
                     /locus_tag="VW006_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9379071.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            65113..66390
                     /gene="tilS"
                     /locus_tag="VW006_000145"
     CDS             65113..66390
                     /gene="tilS"
                     /locus_tag="VW006_000145"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9379072.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            66394..66936
                     /gene="hpt"
                     /locus_tag="VW006_000146"
     CDS             66394..66936
                     /gene="hpt"
                     /locus_tag="VW006_000146"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9379073.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            66952..68910
                     /gene="ftsH"
                     /locus_tag="VW006_000147"
     CDS             66952..68910
                     /gene="ftsH"
                     /locus_tag="VW006_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9379074.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAHHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            69032..69511
                     /locus_tag="VW006_000148"
     CDS             69032..69511
                     /locus_tag="VW006_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9379075.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            69606..69677
                     /locus_tag="VW006_000149"
     tRNA            69606..69677
                     /locus_tag="VW006_000149"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69639..69641,aa:Glu,seq:ttc)
     gene            69929..71474
                     /locus_tag="VW006_000150"
     rRNA            69929..71474
                     /locus_tag="VW006_000150"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            71526..71598
                     /locus_tag="VW006_000151"
     tRNA            71526..71598
                     /locus_tag="VW006_000151"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71559..71561,aa:Ala,seq:tgc)
     gene            71723..74624
                     /locus_tag="VW006_000152"
     rRNA            71723..74624
                     /locus_tag="VW006_000152"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            74701..>74799
                     /gene="rrf"
                     /locus_tag="VW006_000153"
     rRNA            74701..>74799
                     /gene="rrf"
                     /locus_tag="VW006_000153"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        23475 a        15006 c        13537 g        22781 t
ORIGIN      
        1 taatgttata aagcatggta cgagctgcat ttgagtagtc agtcacgtga gccgcaccgt
       61 cagtcaattt ccaaaccaac caagtatcga tagtaccaaa gagcaattcc cctttttctg
      121 ctcgctcttg agcaccttct acatgatcca aaatccaacg aaccttggta gcagagaagt
      181 aagcatcaat aatcaaacca gtcttttcat ggaatttttc cacataacct tggcttttta
      241 gttgctcagc caaaggtgct gtctggcgtg actgccaaac gatagcattg tagataggaa
      301 gtcctgtttt cttatcccag acaaccgttg tttcacgttg gttggtaatc ccgattgcct
      361 cgatttgatt tggcttgaca ccactttcga tgaaagcacc cgcaataact gactgaacag
      421 agttccaaat ttcattggca ttgtgctcaa cccaacctgc ctgagggaaa atctgggtaa
      481 actctttttg actcgagcta accttttccc cttttttgtt gaaaatgatg gcacgagaac
      541 ttgtagttcc ctggtcaatg gccatgatgt atttttcttg tgacatgtac tgtcctcctg
      601 ataaagatct tgaggtgttt cctctttaca cttactagta tacaaaataa agcgctttct
      661 tgtttatcaa ctttttttaa tttttaaaaa aatgtgagga gattgtgtga atttcacaaa
      721 aaagacctgg attaaccaag ccttttaagt gaataataac tggcgaatcg ctaccaaatc
      781 ctccatatcc aagtcatttt ttaaataata gactggtgtc tgttccttct tatgaatcgg
      841 gttattggta accagcaaat cataatgccg actagggtca taggcttcaa tggtaataaa
      901 acgattgtat tccaaatacc gtttcaaaat ggctgccatc cttgaaaaga caagaaaacc
      961 agatgtcaaa tccagaccaa tccgcatatg ctggccaccg ttttcagcca tatattcgat
     1021 taactggagc cattcccaga gaatcttctt atctaaatct gtcccctgtt gaaaagcagg
     1081 tagagcatga aaaatctcct ctgctgtctc tttaaaatca tgttccatca gtaaatatgg
     1141 atgacgattc tctaactggt acttgtagcg atcctgtaaa atatagccct tatagagata
     1201 gacttgagca caaagctgac tgagttcata ggtgacatgg tcctctgtat aatcccccaa
     1261 tagttcctcc ttcttcattt cttgaatcaa ttgcgtcagt aaatctgcca actgccctcc
     1321 aaaaccaaga atatactcca tagtatgaag aggaagaatg cgatgagata ggagaaaaga
     1381 gaagaatatc atcatctcac catcctcaac tcctagtgga atatgttgcc cagcaaaaaa
     1441 ggacggaacc tttctcagca aacgaaggta gaaaatattg tcaaaatacc ctcgcatttt
     1501 ctcttctatg acttgaaact gacaagcatt gacccgaaga cgttgttgac tgatgtgagc
     1561 ccagagaacc aagtccaatt tctgccccac agacaaactt gcaccgcaga tttcctctaa
     1621 attggcaatc tcctgttttc tctctggttt ctgcatgtga ccttcccatt cctgactcga
     1681 ccagaccttt cggaaaagac agaaatagaa atagcgaatc tgatgctctg gacctcgcca
     1741 acgtccattt tggatggata aatcaaattc tgacaaaatc tgatttaaac cagccaagtg
     1801 acgaccaagc gtagcctcgc taatcaccaa ttcttgagcc agctgatggg ctaaaaactg
     1861 ttggtggtag agaagataaa ccaaaatctg gtatttaaca gcactctcca aaaacaagct
     1921 ccgaatatct ctccccttgg tagctgcacc gatagacaga cgcagatttt catcttctga
     1981 gtgaatagcc agctctaagc cactatccaa agccttgtca ttgagcaggg tgacatattt
     2041 ggttagggtt gcttttgaaa atcctgtttc ttccattaca gccttgacgg tcgtctgaga
     2101 ctcttgtaat agaaaggaaa ggattgaaaa ttgaccacac tcggctttct ccatcaaatc
     2161 tcctaaatac atttgttacc cctttcattt tctaccttaa gcatagcata aatcttacaa
     2221 atgctaaaat aatctgtttc tctttttatt ttcatgctga tttcctggtc cattatcctg
     2281 aaaatcagta aacacacggc tccccctttg ggcattttta tgctaaaata gtagctatgg
     2341 ataaaattat taaaactata tcagaaagcg gagcctttcg tgcttttgtc cttgatagca
     2401 cggaaaccgt ccgcactgct caagaaaaac atcaaaccca agctagctca actgtagcgc
     2461 ttggtcgaac tcttatcgcc agccagattc tcgcagccaa tgaaaaagga aataccaaac
     2521 ttacagttaa ggtgttgggc tctagctctc taggtgctat tatcaccgtc gctgatacca
     2581 aggggaacgt caaaggctat gttcaaaatc ctggtgttga catcaaaaag actgcgactg
     2641 gtgaagtcct agtcggacct tttgttggaa atggtcaatt cctcgttatc acagactacg
     2701 gtgctggaaa tccttacaac tctataactc ccctcatctc tggagaaatc ggtgaagacc
     2761 ttgcctttta ccttactgaa agccaacaaa cgccttcagc ggtcggcctc aatgtccttt
     2821 tggacgagga agacaaggtc aaggttgcag gtggtttcct agttcaagtc ttgccaggag
     2881 ccaagaaaga agagattgct cgctttgaaa aacgcatcca agaaatgcca gctatctcta
     2941 ctcttctcga aagcgacgac catatcgaag ccctcctcaa ggctatctac ggggacgaag
     3001 cctacaagcg tctttctgaa gaagaaatcc gtttccaatg tgactgtagc catgaacgct
     3061 ttatgaacgc tcttgccatc cttccaagct cagacttaca ggaaatgaaa gaggaagacc
     3121 acggggtaga aatcacttgt caattctgcc aaactactta caactttgat gaaaaggacc
     3181 tggaggaact cattcgtgac aaatcttaat acacctttta tgattggcaa tgttgagatt
     3241 cccaatcgta ccgttttagc gcctatggct ggcgtgacca actcagcctt tcgtaccatc
     3301 gcaaaagagc tcggagctgg actcgttgta atggaaatgg tctctgacaa gggaatccaa
     3361 tacaacaacg aaaaaaccct gcacatgctt catatcgatg agggcgaaaa ccctgtctct
     3421 atccaacttt ttggtagcga tgaagacagc ctagcacgcg cagcagaatt catccaagaa
     3481 aacaccaaga ccgatatcgt cgatatcaac atgggctgcc ctgtcaacaa aatcgtgaag
     3541 aacgaagctg gagctatgtg gctcaaggat cctgacaaga tctactctat catcaacaag
     3601 gtccagtctg tccttgatat cccacttact gtcaaaatgc gtaccggctg ggcggaccca
     3661 tctctggcag tagaaaatgc cctcgctgct gaggctgcag gtgtttctgc cctcgccatg
     3721 catggccgta cccgtgaaca aatgtatact ggccacgcag accttgagac cctgtacaag
     3781 gttgcccaag ctctaaccaa gattccattc atcgccaacg gtgatatccg tactgtccaa
     3841 gaagccaagc aacgcatcga agaagttggt gctgacgcag tcatgattgg ccgagctgcc
     3901 atgggaaatc cttacctctt caaccaaatc aaccattact ttgaaacagg agaaatccta
     3961 cctgatttga cctttgaaga caagatgaag atcgcctacg aacacttgaa acgattgatt
     4021 aacctcaaag gagaaaacgt cgcagttcgt gaattccgtg gcctcgctcc tcactacctc
     4081 cgtggaacat ctggcgctgc caaactccgt ggagccattt cgcaagctag caccctggca
     4141 gagattgaag ctctcttgca attggagaag gcttaatagt ttaaaacccg taactctctt
     4201 aaagagtctc ttgagtgccg ccattctttc ctcatcaaaa aaactccgta gtctctagcg
     4261 ctataaatcc ggctagagat tacagagtta ttttttaatc taaacttcct acaagggtat
     4321 attatctact gaaaggatat ggtatttgta gggaatactt taaagagttc ttacaggaag
     4381 ccagcattaa cgagttatat taggtttagt ttacccattc accattggca ttgactctat
     4441 agctatctac agttgtgttg actgcaaggg cacctgagcc attgacatag taccatttat
     4501 ctgatacttt gaaccattgg cttgctttca tagcacctga tgcttcaaga tagtaccagg
     4561 tatctccatc tttcacccaa tctgttgcca ttgaaccgtt agcgttgagg tagtaccatg
     4621 aaccattgta ttggagccat cctgtcgcca ttgaaccatt agcgttgagg tagtaccatg
     4681 agccattgta ttggagccat cctgttgcca ttgaaccatt agcgttgagg tagtaccatg
     4741 aaccattgta ttggaaccat cctgtcgcca tatcaccatt agcgttgagg tagtaccatg
     4801 aaccattgta ttggagccat cctgtcgcca ttgaaccatc agtattgtag aagtaccaca
     4861 taccgttttc ttgtttccag cctgtttttg gagtagatgg ttgtgctggt ttttcagttt
     4921 ttggcggttg ctgttgagtc aagcgattat attcttcttc tgatctacga gcatagtctt
     4981 cttcagcttg ttgatcagct ggtttttctg cttttggttg ttcagctgga ttctctggtt
     5041 ttggagctgg agctggtttt tctggttgag gagccggcgc tggttgtggt tgttcagctg
     5101 gtttttcttt aactttatct tcttctgctg cttttcgttt agcttcttct tctgcttttt
     5161 tacgatctgt cttgattttt tctaaccttg tagcctcagc ttttttactc tcaacttccg
     5221 cttttgcttg cttaactttt tcctcgtttc gagatccctt agcttcctct tttactagtt
     5281 caagctccgc ttttttaact tccacatcgg actcagcaat ttcaagttca agcgttttgt
     5341 aagtaatggt tgggtagtta cggtgatctt cttctttttg atccttggct tttttctcag
     5401 cttcttcaac cttcttctca gcttctgcta ccttttttcc tggtttcagg gatgggcttg
     5461 gaagagtttc ttcacctacg ctagaatctg aagacttcgc atcattttct tttttatcag
     5521 gtgttgctgg ctctccaaga tctcctcgtt ttacccgact ctttcgcctc tttgattcat
     5581 cttgctcttt agcatctgct cttcgtttag cttcttcttc tgctttttca cgatctgtct
     5641 tgattttttt taaccttgta gcctcagctt gttcactctc aactttcgct tttgcttggt
     5701 taattgtgcc ctcgtttcga gattcgtcag cttccttttt tactagttca agctccgctt
     5761 ctttaacttt cacatcggac tcagcaattt caagttcaag cgttttgtaa gtaatggttg
     5821 ggtagttacg gtaatcttct tctttttgag ccttggcttt tttctcagct tcttcaacct
     5881 tcttcttagc ttctgctacc ttttttcctg gttctgttgg taatgtatct tttttaaact
     5941 gctcaaaagc tgcgtctaac tctgctttta tttttgacgg caactcagct ttcgacttat
     6001 cttctaaaac atttaattca tacaaatacg ttctcttaat gtcttgcaat ttgttaatta
     6061 gatttacagt tttgatatgt ttacttctat ctaaatttgt ttggatctca ctcaatattt
     6121 tttctagatg tgacttgact tcatcagcat acttcttcga tatatcttgc ccactagatg
     6181 taaccgtggg gttattctca cttctaaccc cttctgcatg gactactcct cctaagaaca
     6241 agctagcaac tactacacta gctactccaa tactaaattt acgaattgaa taatgtactt
     6301 ttctttcgct ttttgatgca aacatgttta tttccttcta tattttttct ttaaccttac
     6361 tatatcactt caatatgaac tgtatataaa tcgaattttg tatttatctc tttatgcttc
     6421 catttgggat tataacttca tgtaatagct tttcctcttc agctattcgt cgatacaaaa
     6481 taactgcata aactggaaaa actaaaacaa aagtcacata agcatgactc aacaaggtca
     6541 agccaatcaa ctcaggtaaa atatttaaaa agtaattagg gtgttttact gtcctaaaca
     6601 agatatgatc tacatatttg tgattattga caagcataag cttcactgtc caaatatctc
     6661 ccaacaagtg aatcaccaac atcaacatca gcatagaaaa aataagcaag accaaaccaa
     6721 ccatgcccat gccatcaaaa attgtcttgt gcaccattgc ctcaaccaag caagagagat
     6781 aaaaaacaat atgagccata gttaatcgtt ttgtattctc aactccaaat tctttcccgc
     6841 cattgctaag gattcgtttc tcattctcta tcgaacgctt taaaaaaatc aatcgaataa
     6901 agaatgttac taaaataatc gctaatacca tattagtatc ctttctttta tctacacaaa
     6961 gaataacaca cttatgttaa ccctatatga actttaataa aaaactaatc tgtctacaag
     7021 ctaatcttaa attccatacc gtgttcataa gggagaaaac tatagtccat ttgatagctt
     7081 tctaaaatat tattcacaat gtaaagaccc aaaccagaac tttcatcctt atctgtcacg
     7141 atttgagaat gatagaatat atcaaataaa tgttctagtt cttgatcact tagaggcgca
     7201 catgtatttt tgatagagag atagccatct tgctcggata tgattacgcg cccattttta
     7261 tctgaatatt taattgcatt actaatcagg tttgtcaaaa ccttatccaa tgccctaaga
     7321 ctcatgacga ccctggtagc atctgtcaat tgattttcaa ttgtaacacc tattgaatga
     7381 gctaattctt gataacgtga taaaatatct actaaaactg gcttaacagt caaggtctca
     7441 cgacactctg tccactcttg gaatttagaa gactccagta cttcttccaa taagcggctc
     7501 atctggtcaa tcttatttat actctttgca atatattttg gatgatcttt gtaatctcca
     7561 atattatgct gcatattctc taggataatt ctaagactgg ctaaaggggt tttcaactca
     7621 tgtgatgctc cacggacaaa ggaaaccttt tgattttgca attttacaat ttgctcatta
     7681 cgactttcca actcatgaat aaccttcaac aagtgctcat acataccatt aatctgtttt
     7741 ccaacttcac caacttcacc aacttcatct ttcctacttt catcaaaacg aatattgtca
     7801 tccaaatctt gcattttact agtcacttct gaaatgtaaa aaagaggatt gagcaagcgt
     7861 ttagtataaa aataagaaaa aacaaaggaa aaagcaattg taaccaaaaa tgtatatggg
     7921 agatacaaaa gcaaaatatt ctttgcttct ttgtagacat ccaccccgtg aacaaattgc
     7981 acggttacct gtttaccatc tgctgtacta acagacatat ctaacatcac gatataattt
     8041 gatagctttc catctttaat atccaagtca tgcacaagag ggagccgctt atctacaata
     8101 tctcttttaa ggtgagcaga tatggtcaag gactttgaat aagactggag taagcttgga
     8161 atttcatcag atgtcttagt ctttaaatac tccgtaaaca ctcttgcgct ctcattaaac
     8221 ttttccccct ccagttgaat ataataaaaa ggaaaggtca aataaatagc tatatgaatg
     8281 actagaccaa caccacctag aattgcaaaa cttcttaaaa aagacttggt taataattta
     8341 ggatttttta tcataagcta atcttatacc caacattttt cacagtcacg atacaatcta
     8401 aagctaattt tttgcgaaga gtacgaatat agacatcaac gaccctatca caaggtaaat
     8461 cagctacatc ttttgaaatt tcttccaata tctgctctct acttaaaact tgatttttat
     8521 gctgaatcaa acactttagt accagtaatt cctttggttt gagatcaatt tcttcatttt
     8581 tataatgtgc tttgtaagag gtaaaatcca ctgttacatc ctgatatcgc caaagatcct
     8641 ctatgacgta gtaacgtctg ataagcgcct taatacgctt gacaagcagt accaaagaaa
     8701 aaggttttgt cacatgatca tcagcataga gatcaaatac ctgtgactga gtagtttcat
     8761 ctcccagagc actcaagacg atgacaggaa tatcgtgctt ttgattgatt tcttttaaca
     8821 cttcaaaacc actcttccta ggcatcatta aatccagtac catgagatgg atagggagat
     8881 cttgaaattt ttccaagacc tcttgtccat ccttagccat aatgacatga taaccctctt
     8941 ctgtcagaaa tgctgcaatt ccttctctaa tcatttcctc gtcatctgca actaaaatgt
     9001 tcatctctct ccctttctac taccagaact ccatacatct attttctgta tgctatttga
     9061 aaatttccta ccagcctatc atctatgata atggaatgga tttcatttat ccgctacttt
     9121 ttgtcaacaa caacgtcaat acaagaaaat cattcatctt gcagcttata ctcttcaaaa
     9181 atctcttcaa accacgtcag ctctatctgc aacctcaaaa cagtgttttg agcaacctgc
     9241 ggctagctgc ctagtttgct ctttgatttt cattgagttt cactccactg tgacttatgg
     9301 tcagatgctg gtacttttat tcttttatgc aatatcaaat tttaactggc ctgctttgac
     9361 accaatctta agtgtgctgc ctgccactaa atctcccttg agaagaagtt ctgccaactt
     9421 gtcctccact tctgtttgca gggttctgcg aagtgggcga gctcccatct ctgggtcata
     9481 tccttgattt gctaacaatt tcagagctga agcttgtaat ttcaagtcaa tgcctttttc
     9541 agccaaactt gccactaaag gcttgaccat aatcttcacc acttcctgca tatggtcact
     9601 agataggcta tggaagacca ccttctcatc aatacggttg atgaattccg gtctataagc
     9661 ttttttcagt tcttcaaaca tgcgtttttc catattttcc tggtcaaaac gaatatcctt
     9721 agccccaaaa ccaacagtct tatcatcacg aagggcagtc gcacctaagt tcgatgtcat
     9781 aatgataatg gtatttgaaa aatcgacctt gcgtcccttg ctatctgtca agacaccgtc
     9841 atccagaacc tgcaagagaa cattaaagat atctggatgg gccttctcta cctcatcaaa
     9901 gaggagaacg gaatagggtt tattgcgaac cttctctgtc aactccccac cttcttcgta
     9961 acccacatag cctggaggag ctccatttag acggctggct gcgaatttct ccatatactc
    10021 actcatatca aagcggataa gggctgattc gtcatcaaag agaacttctg ccaaagcctt
    10081 ggccaactcg gtcttaccga cacctgtggg tcctaggaac ataaaggaac cgatcggacg
    10141 cttgtgactg cgaattcctg actgattgcg gcgaatggca cgactaatac ttgaaacagc
    10201 ttggtcttga ccgatgacac gtttgtgcag ttcagcttcc aagtttaagt atttcttggc
    10261 atcagtctga gtcagttttt gaacggggat acctgacaag cgactcaagg tggtcaaaat
    10321 atcagactct gtcaccaagt ctttatagac aggcacttcc tcttcttttg cgattagctg
    10381 ggctgcctgt ttccacttgc catccatcaa ggccttgtca gctggactca agtcagaatc
    10441 gtctgctttt acatgctttg atttattttg cactgttgct gccgcctcat ccaagagatc
    10501 gatagcagag tctggcaagt gacgactggt taaataacga tgagccatct taaccgctgt
    10561 ttcaaccgct tcatctgtga tttgtacacg gtgatgtttc tcataagtcg ccttcaaacc
    10621 ttgtaaaata gtcatactat ctgccacact tggttcttca atcgtcactt tagcgaaacg
    10681 acgagaaagt gccgcatctt tttcgatatg tttttgatat tcttcctgag tggtggcacc
    10741 aaccgttctc aaagttccac gcgccaaggc tggtttcaag atattggccg catccagagt
    10801 cgaatcaatt ccgctaccag aacccatgat ggtgtggagt tcatcgataa agaggatgac
    10861 ttggccatct tcttcaatat ccttgatgat attattcatg cgttcttcaa agtcaccacg
    10921 gaagcgtgtc cctgcaacga cattcatcaa atcaagttct aacacgcgca tcttagccat
    10981 ttccgcaggc acgtcaccac tagcaatacg ctgggcaaga ccaagcgcca gagctgtttt
    11041 cccgacacca gcatccccaa ccaagacagg gttgttctta gtcttccggc tcaagatttg
    11101 aatcatacgt gagatttcct tgtcccgacc gatgactggt tctaacttgc cagaacgcgc
    11161 ttgctctgtc aaatcatgcg tatagtcctc gagaccacca ctaggagtct gcggcatgcc
    11221 catcatattg gccatagaat tttgcttgtc agctactgta cgatggcgtt ggcgtaaagc
    11281 cttgagatct tcacgagtcc agcctgcccg ttcttctaaa tttcgacgaa gagcagcaat
    11341 cttgacctga tctttcttgt cttcataaga aaatccagcc ctctccaaga tacgagtcgc
    11401 caaggcattg ccatcatgca aaatcgcata gaggacgtgc tctgtcccta gcaccttagc
    11461 atggaccact gacgctacat actctgcttc gtcaaaaaga acctgcaaac gacgggagaa
    11521 cggcaattcc gtaaaggttt catcctggct atagtccgtt tcagtcagtt ccaaagccac
    11581 ctcttctaaa cggtccatct catacggata atcatttaaa gttgcccctg ctacactata
    11641 actgtgatta gacatggcaa tcaacaagtg ccaagactct agataacgag ctccaaaatg
    11701 tccagcaacc atgtaggcac tttcgataca ttcattcaat gcttttgaat agttcatctt
    11761 acttcccttt tctatctacc tcttgtatga tctgacgtag catgtttgct cgaacaactg
    11821 gagcttcttc tcctaaaacg cgatccaaag ctactgattc tagcaaattc atctcctgct
    11881 tggtcatcaa ttcctgctca accaaaagct ggagaatatc ctcataaatt tcttgactga
    11941 ctcgctcacc aatcgagtaa agcagctccc ggagcatttc atgatgacta gaaaactcaa
    12001 tccgtcctat acgaatgtag cctccgccac cacgcttact ttcaaccaag tagcctctac
    12061 tttccgtaaa gcgtgtcttg atcacgtagt taatctgact aggaacaacc tgaaaggtat
    12121 ctgccaactg actccgttgc aactccacga taccagattg atctaaaatc gccttgatgt
    12181 aggcctcaat atgatccgat gtatttttaa atctcataac aaacccacct ctctctttaa
    12241 accttgacct tgtttgacta ttataccgaa attttctcat ttttaaaaga aaaagggcgc
    12301 tggtaaagga taatcttcac caactcccta tttttctact tatctaagcc taattctgcc
    12361 aagatttgac gtttgtaggc aatcttttgg acttccttgt cctcatcttc agaccaatct
    12421 agtttaattt ctgaaacaat ctgcccaggg cgatttttca agatatagat gcggtcgctg
    12481 agattgaggg cctcctcaat actatgcgtg atgatcaggg ttgttagctg caactgcttg
    12541 tgaatctcaa gataccaagc gtggagttcc atctttgtca tctcatccaa ggcgctaaag
    12601 gcctcatcta agagaaagag cttgtgccca aaaaggtagg tccggagtaa ggctacacgc
    12661 tggcgcatcc caccgctaag ttcatgagga tacttgtctc ttacagctgt cagctggaag
    12721 gtcgcaagaa ttttatccgc tcgggaaata gcttctgcct tatccacctt ttgaatcaag
    12781 aggggcagaa tgatatttcc aagcaccgtc ttgtgctcca agagcagatc cttttgcaac
    12841 atataactca cgcgcccctt gggattttct tcaccatcaa ggacaattct ccctgactga
    12901 acttctaaaa tcccagcgat tagattaaag agggtggtct ttccaacacc acttgggcct
    12961 aggatggaaa ccacttcgcc tgaagtcacc tgtaggttga tatcctctaa aatcctctcc
    13021 tgaccatagg cataactgac gtgctctagt ctaatttctg tcattatttc acaaattcgt
    13081 tggtgaagcc tttgtctgtc aagtcttctt taaggatacc attttcttta tcccatttgt
    13141 agaaagcatt ccagcgagct gcgtcaaatt gaccccattt ttccttgtcg cttgcgtatt
    13201 cttttgacaa gtatttttga gattcgatga caaagtcacg tttttccttg agttcaggtg
    13261 cattcttgat gagaatatct gcagcttctt ctggatgttc catggcatat tggtagcctt
    13321 ttttgatggc ttggatgact ttgcgagctt cttctttgtt atctttcaga tagtcgttgt
    13381 ttgcgatgat aactggtgaa tagtagtcaa actccttgac atagtctttc aagtacatga
    13441 agttagcatc tacaccttga gatttagcaa ggataccatc ccaaccgtag taaatccaag
    13501 cagtatcaaa gacgccattg gcaatcggtg tgattgagtt tgagtcgtta tttggtactt
    13561 tttcaacctt ctcaaagtct ccaccttgag attctaccaa ggttttcaac atagcaagtt
    13621 cagttgggtc attccatgtc ccatatttct taccaaccaa gtcttttgga ctgcttacat
    13681 tatcagattt acgagagatg attcctgatg tattgtgttc aacaatagct gcaacggcag
    13741 tgattcctgc tcctttttcc aatttcttag ccatgtagtc ttggaaatac actgcaaatg
    13801 gtgcctttcc gttgataacc aagtcagaag aactttcttc tggtggcaat ttcaaatcaa
    13861 catccactcc agcttctttg aaataacctt tttccttggc aacataaagc cctgtgtggt
    13921 tggtatttgg tgtccagtct aggataaagt caaccttctt aagttctgcc tctttgttgt
    13981 ctttagaagc agttccttga ccacaggcca caagcacaac agctacaaga gctgttacaa
    14041 gcgttaaaaa cactttccat gttttcttca ttttgatttc ctcttttctt tttcaaaaac
    14101 gctctatttt aagtacgttt ccatttaatc acatattttt cactgatatc gaccagcttc
    14161 atacccaaaa gactgataat cgacaccaga ataataatgg caaacatggt atcatactga
    14221 aacagttttt tagactgaat catataaaca ccaagacctt caaaacctcc caaccactca
    14281 gataccacag ttgtgataaa ggcgtaggag acactgaccc tcagacctgc ataaaagtaa
    14341 ggcaggctaa ccgggatttt aaaatgccac aggatttgcc aagggtttgc tcgcatcaga
    14401 ctaaacaaag tcagcatatc cttgtcgcaa tgcctaaaac cgtccaaaat actaacgatg
    14461 atgggaaagg ttgttgttaa gataatcaag acaatcttgg gcaaaatccc ataacccagc
    14521 cacaaaacca ggataggagc tatggcaatg gtcggaatgg tctgaatgac caccatcata
    14581 gggtaaatca ggtcattgag ccaagtcaaa ctatccatga gcacagccat aagacaggca
    14641 atcaaaactc ccaaaatcag ccccagtaaa gccactctca aggtcgccca gctatggtgc
    14701 cagagaaatt ctctgtcacg aacaaagggc tggagaattt caagaggtgt cggcaggata
    14761 aacttgggga gaagtttaag aaaacctgct aactgccaga ttgacaatac tccgagaaag
    14821 cccaatagac taatgtgtcg tctcagtata cttttcaagt ttctcatcaa tacttaaaat
    14881 ctctcctaca tttattttga cattggcaaa gacattgtct gcctcctgcc ctgccacttc
    14941 cagcgcttct tttagaatgc gcataagctc atcaaactcc ccttccaaga ccgtttcaaa
    15001 tggtgtcact accatcgtca cttcttgagt ttgcagataa gcaatgacct gatcaataac
    15061 agctatccga tcaatcccct gtgctagggg taaaacttgc aaggcaatgc ttgctttcat
    15121 aagaacctcc tcttctcgtt ttccttttaa aaaaaagaaa aacctttacc atacatggaa
    15181 aaggtgcaaa attagactaa gtatcgttcc ctccgctggc attacccaga tcaggtgcgg
    15241 tcgaagttta acacttcctc tcagactgta cacagactcc catatattat atgtatatat
    15301 gatagcgcaa aataagaaaa atgtcaagga aactgcttac agaaaagagc aggaaatttc
    15361 tcctactcca attttctata ctcgttctcc atcactgttt actctgtagc catccaccgt
    15421 cgtattaaca gctaaggcgc ctgaagagta agaataatac cacttaccag agacctggta
    15481 ccagcctgtt ttcattgccc ctgaactacc cagataatac cacaaaccat tttcctttaa
    15541 ccatcctgtc tgcatttcac cagataattt caaataatac cagctagaac cgtctttcag
    15601 ccatcctgtt gatttagaac catttgattt gaaatgatac caactaccat ttattttctt
    15661 ccaaccgaca gttgaactat tattggatcc atagctagag atatttcctg attgattctg
    15721 ataattttgt gaagaagtca ctccctctag ctgtatatag cgacgacttc cgttataagc
    15781 cgtataactc aaccacttgt agccgtcttt ttcgagaatt tgatcataat gaaccttctc
    15841 tccaggataa tagtaatcaa tcacagttgc actaactagg ggttcagttt taatagcaga
    15901 cttggtctta aaataatgag ttccgcctgt tgaagttgag gattgactat tcccaacact
    15961 gccaccatcc aaatctttaa aatgaataaa tcctgtcatc gtgtttgctt ttataattcg
    16021 tttattatag gattctgtat aaccatagtt atattcctca atctcaatct gatctcccat
    16081 tacatttgac acccaggcaa catgaccata agttcctgca gtagaccaag taatggaacc
    16141 aatcgtcggt gtattatcta cacgataacc ttcccgacga gcacgatgtc cccattcatt
    16201 cgcatttcca taagctgccg gaatttcaaa accattgaca ttactcaaac gaaaggctac
    16261 aaaagaagta cactgacgag aatacatgcg ccactgatca atctcctggc tcccattttt
    16321 ataataagca ggataatcat ccccacgcgc aatcgatcca tttcctccgg aataggcata
    16381 gacactatca ccagctgcta gcatcaatcc tactactaaa aaaggaacaa gctttttaac
    16441 tgacatcttc aagtaataac ttgttcctct tgctataaac ggtaaaattt tcattcttcc
    16501 tccttgaaaa ataatataaa gcgatgattg ccttcaccta aattattcgg agaaaaaaga
    16561 aaaagtgaga aaatttctca ctttagtcaa gataatgaat caaattcttt tctgtaagaa
    16621 tatctgagta agtaaaggat tcaacgtaaa cattaacttg tttatctcct tccacatccc
    16681 caaactccac aataaatagg gatggataaa cttcaattag cttacccaat ctattttttt
    16741 ggcgcttacg accattctcc aaagtcattt ctacgacttg tccctcatgt gccttgattt
    16801 cttctttgat ttttttcatc ttggctacat ctgtaaatgc atctgacatc ttatgcctcc
    16861 ctctttgaga tacttgaaaa tttattgtat tctttttgga aaatcaattc cactgttcca
    16921 cgagctccac tacggttttt ctcgataata acttccacct tattatttgg gataccctcc
    16981 tcttcttcac caccacgttc atagtagtca tcgcgataga gaaaagctac gatatcagcg
    17041 tcctgctcaa tagacccaga ttcacgaata tcagacaaga ccggtctctt gtcctgacgt
    17101 tgttctacac cacgagaaag ctgactcaga gcgattactg gaaccttcaa ttccttggct
    17161 agtattttca actgacgaga aatttcagaa acttcttgtt gacgattttc tcgaccagtt
    17221 cccgtgataa gttgtaaata gtctaccaaa atcaaaccaa gatttccagt ttcttgagcc
    17281 aatttacgag aacgagaacg aatctctgta atccgaatac ctggcgtatc atcgatatag
    17341 atactggcgt tagctagatt accctgagca atagtatatt tttgccactc ctcatctgtc
    17401 aattgccctg tacggataga atgtgactcc accaagcctt ctgcagctaa catacgatct
    17461 accaagcttt ccgcacccat ttcgagtgaa aaaatagcaa ccgttttgtc caacttagtc
    17521 ccaatgttct gagcgatatt caaggcaaat gctgtcttac caactgctgg acgagctgct
    17581 aagataatca actcctcctc atgaagtcct gttgtcatat gatccaaatc acgataacct
    17641 gtcgcaatac ctgtaatatc ggtcgtttgt tgcgagcgag cttccagatt tccaaagttg
    17701 agattcaaca catctcgaat gttcttaaac ccgcttcgat ttgcattttc actgacatca
    17761 atcagccctt tttctgcctg agcaataatt tcatcagctg gttgtgacgc ttcgtaagct
    17821 tggttgacag actctgtcaa cttggcaatt aaacgacgta gcattgcttt ttctgcaaca
    17881 atcttagcat aatactccgc attagcagaa gttggcacag aattaacaat ctcaaccaag
    17941 taagacaagc caccaatatt ctgtaaatca ccttgattat caaggatagt acgaaccgtt
    18001 gttgcatcta tggcatcacc acgatcggat aaatcgacca tggcttggaa aatcaaacga
    18061 tgggcatact taaaaaagtc ccgagactca atgtattctc gcacaaaaac aagtttactc
    18121 tcatcaataa agatagcccc taaaacggat tgctcagcta agatatcttg aggttgtact
    18181 cgtaactctt ctacttctgc catcagactt cccttccttt tacaatcttg tcaagaaggt
    18241 gtaaacttat ccttctttca cacgaagatt gattacactt gtgatatctt gatagatttt
    18301 cactggcaca tcaatcaaac caaccgctcg aatcggagct tgtacttgaa tatgacgttt
    18361 atcaatctta attccaaatt gcttttgcaa ttcttctgca atcttcttat tggtaataga
    18421 accaaaggta cgaccatctg gaccaacttt ttcaacaaat tctacaacag tttcttctgc
    18481 ttcaagttgt gctttaattg cttttccttc tgcaatcatc tcagcgtgag ctttttcttc
    18541 cgatttttgt ttaccacgaa gttcacctac agcttgagca gtcgcttctt tggctagatt
    18601 ctttttgata agaaagtttt gcgcataccc tgttggtact tccttaattt cgcctttttt
    18661 accttttcct ttaacatctg ctaaaaagat tactttcatt cttctttctc cttttccttt
    18721 atttcattta atacaatttc tgtcagtttt tcacctgctt ctgacaaggt tacatcttta
    18781 atttgagctg ctgccaaatt aaagtggcct ccaccgccta actcttccat aatccgttgt
    18841 acattcagtt tactacgact tcgagctgag atagagataa atccttgtgt attcttcgca
    18901 agaacaaaac tcgcttcaat acctgacatg gctaacatgg catctgctgc cttactaata
    18961 acaactgtat catagcattt cgtgtcctta gcctctgcta ttagtacatc tgaacctaat
    19021 ttacgcccct gtaaaataag ttcattgacc tcacgatatt cttcaaaatc tgtcgcagcg
    19081 atttcctgga tagcaatact atcacttccg cgcgttctga gatagctagc aacatcaaat
    19141 gtccgactag ttactcgcga ggtgaaattt ttagtatcca acatcatacc agccatcaaa
    19201 acacttgctt gcatacgact caaacgattt ttcttagaat tctggaactg aatcaattcc
    19261 gttaccaact cactggcact acttgcacca ctttcgatat aagtaatgac cgcattatct
    19321 ggaaaatcct gatcccttct atggtggtca ataacaatgg tttgggtaaa taaatcataa
    19381 aattcttttg ataatgttaa ggctgtcttt gaatggtcta caagaatcaa caaagaacga
    19441 ttggtcacca tccccattgc atccttaaca gacaacaact tcgtaactcc ttctttttct
    19501 atgaatgaaa cagctcgttc aatatctgga gacatttgtt cttcatcata aagagcatag
    19561 ctattttcaa tcacattgct ggcgaacaac tgcataccta cagcagagcc caaagcatcc
    19621 atgtctaaat ttttgtgacc gactacaaaa acctgatcta cactccgaat cttatctgaa
    19681 atagctgtca tcatagcgcg cgtacgagtc cgtgtacgct tgattgaagc agcagaccca
    19741 ccaccaaaat aaactggatt tttcgtttcg tcgttttcct taacaaccac ctggtcgcca
    19801 ccacgtactt cagccaagtt caaattgagc aaagcaactt tccctatctc atcatgattt
    19861 ccatcgccat aagaaaatcc catacttaag gtcaagggca actgtctctg tttcgactct
    19921 tctctgaaag catcaataac agaaaattta tcattcatca agccctcaag caccgtgtag
    19981 tcagtaaata gataaaatcg atccatactt acccgacgag aaaacatcat gtatttttct
    20041 gaaaactctg atataaaatt agctacaaaa ctattgattt gactaatatc tgactcagaa
    20101 gttgcatcct ccaaatcatc ataattatcc acagagacaa tcccaatcac tggtcgactt
    20161 gttaccaatt catctgttat ggcttgttcc ctggatacat ctacaaaata caaaacaccg
    20221 gaagaagcat ccatatgaac agcataacgc ttctcaccaa gcttggcata agtagacgga
    20281 tttcctactg aagccttgat aatcgtttga acagcttcta aatcaaaatc accatcttcc
    20341 ttggtcaaaa tcaattcagc atagggatta aaccactcaa cctctccaga agataaattc
    20401 aatttcataa cacctacagg catctgttcc aatagagctg tcaaactttc ttccgcttgg
    20461 tggtttacat actgtatctg ttctacatca ctccttgtat aatgcactct cagtttctta
    20521 aataaaaaaa catagcctcc tacaaaaaga aacaaaatta aaaccgtcaa cagattatta
    20581 ttaacaaaaa taatgaaagt ggataagact ccaaacgcaa tcaatcctac tagaatagga
    20641 aaaattggac ttacataaaa ttttttcatt caaaacctct tggcacccat tataccataa
    20701 tacccctcaa aaagcgactt tttaaaagtg taatcagtaa ttctatcaat tataagaaaa
    20761 aggtagttta caattcagta aacctacctt tacacatatt gaaattaaga ttctttaacc
    20821 tctaacaaac caatttcgcc atcctcacga cgataaatca cattggttgt ttgatcttca
    20881 acatccacat agataaagaa atcatgcccc aataaatcca tttgtagaat tgcttcttcc
    20941 aaatccattg gttttaaatc aatttgtttt gaacgaacaa ctttagactg gacaacattt
    21001 gaatcttcca ccaaagcatc tgtaaataat tgaccagttg ctaccttatt tttattttta
    21061 cgctcgattt ttgttttatt tttacgaatc tgacgttcaa ttttatcagt tacaaggtca
    21121 attgaaccat acatatcttg agatacatct tctgcgcgga gagtaataga tccaagcgga
    21181 atcgttactt ccactttagc cgttttttca cgataaactt ttaagttaat tcgggcatcc
    21241 aactcttgtt ctggttggaa gtacttttcg atcttttcga gtttagaaac tacataatca
    21301 cgaattgctt ctgttacttc taggttttca ccacggatac tatatttaat catatgagta
    21361 ccttctttct aaacattttt gtttttatga ttttattata acgctttcat tctatttttg
    21421 caaatttttt cctcatctta caagggaaaa tgtttttaca tccttagcac cagcttcttc
    21481 caacagtttc ttaacacgat ttatagttgc tcctgtagta tagatatcat ctataagtag
    21541 gattttttta ggaatagtga ctccactttt aataaagaaa ggaagttctg tccccaagcg
    21601 ctctgaacga tttttagaag aactggctcc ctcttctctt ttctctaata aatccagata
    21661 ctcaaagcct gctgcctcta ccaagccctc aacctgatta aatcctctat tagcatatct
    21721 atcaggactt aggggaatta caacaaattg atactctttg tactttttca actcctcact
    21781 taaaaatgaa gcgaaaactt ttcttaacag gaagtctcca tcaaacttat accgactgaa
    21841 aaaatccttc atagcttgat tgtaagtaaa aatcgctcta tgactgactt caactcctta
    21901 tttacaccaa agttgacaat cttgacactt tgttgacaac tctgttttca tacaatttgg
    21961 acagttctct tccccaattc tttcaaaagt agaatcacag tctgaacaaa gacaagagtc
    22021 atcattcctc agaagtaaga gactactaaa agttaaaaca gtcttcatag tctgcccaca
    22081 taacaagcac ttcatagacc agcctcctta ttcatcatct gaatttcctt aatcgccttc
    22141 ttgattgaag catttaaccc atcatggaag aaaagcaaat ctcctgtcgg tctatccatg
    22201 cttcgtccaa ctcgtccacc aatctgaatc aaattagact tggtaaacaa acgatgattg
    22261 gcctctacta cgaaaacatc cacacaaggg aaggtaactc cacgctccaa gattgtcgta
    22321 ctgataagta ttgtcagttc tccatctcga aaagcttgta cttgctctaa tcgatcctct
    22381 gttacagaag atacaaagcc aattttctca tttggaaatt gctcctgtaa gatttcttct
    22441 aactgctccc ctttcttaat ttctgaagca aaaatgagta acggataagc tgtctttctc
    22501 tgcttctcaa tataggactt taactttggt gacaaacgat tcttgtctaa gtagcgatta
    22561 aaatccgata accaaattgg ttttggaata atcaacggat ttccatgaaa ccgtctcggt
    22621 aaactcagtc tttttagttc tcctaaacgg acctttttat ctaactcatt ggtcgaagtc
    22681 gctgttaaaa agattctcaa tccattctcc tttacactat tcttgacagc gtggtaaagc
    22741 gtgggattat caacataagg aaaagcatct acttcatcca ctatcagcaa atcaaaagct
    22801 tgataaaact tcaataactg atgggttgtt gcaacaacta gtggtgttcg aaaataaggt
    22861 tccgattctc catgtagcaa agctatcccg caagaaaaat cctgttgcag gcgcttgtac
    22921 agctccaaac aaacatctat gcgaggacta gccaaacaca ctgcaccacc cgcattgatc
    22981 actttagcca ctacttgata aatcatttct gtctttccag ctcctgttac cgcatgaact
    23041 aaggttggct tttgcttgtc tactgcttga agcagtccct ctgacacctt ctcttgaaaa
    23101 ggagttaatt ggccgcgcca tttgagaaca tcttgcttcg gaaaatcctc ctgcggaaaa
    23161 tagtataaag tttgatcact tctgactcgc ttcatcagca agcactctcg acaatagtaa
    23221 gcaccgatgg gcaaatacca ttcttctaga atagtactat tacaacgttg acagaaaagt
    23281 ttccccttct cctttctcat tgctggaagt ttctccgcca actgacgttc ttcttctgtt
    23341 aattcattct cagtaaataa acgaccgaga taatctaaat ttactttcat acttctctat
    23401 tcgtaaaaac tagcacttta gattattttt tagtacaatt aaatcatgga atttaggaca
    23461 attaaagagg acggccaagt ccaagaagaa ataaaaaaat ctcgctttat ctgccatgcc
    23521 aagcgtgttt atagcgaaga agaggctcgt gacttcatta ctgccatcaa aaaagaacac
    23581 tacaaagcga cacataactg ctctgccttt attattggag aacgtagtga aattaaacgt
    23641 acaagtgata atggtgagcc tagtggtact gctggtgttc ccatgcttgg ggtactagaa
    23701 aatcacaatc tcaccaatgt ctgtgtggtc gtgacacgct actttggtgg tattaaacta
    23761 ggcgctggag gactaattcg tgcttacgcc ggcagtgtcg ccttagctgt caaagaaatt
    23821 ggtattattg aaataaaaga acaggctggc attgctattc aaatgtctta tgctcagtac
    23881 caagagtaca gtaacttcct taaagaacat gatctcatgg agctggatac aaactttaca
    23941 gatcaagtcg atacgatgat ttatgttgat aaagaagaaa aagaaactat taaagctgca
    24001 cttgtggagt tttttaatgg aaaagtcact ttaactgacc aaggtttacg agaggttgaa
    24061 gttcctgtaa acttagtgta aacaatgaat aatacagcgt ttcgttgaca ttctcacaac
    24121 tactttagcg agcaaaataa aaagatgcgt accaaaatat actagaaaat gaagcaattc
    24181 aaacgaaacc tgatagcgtt ttccttcaca cctatttact agaattagct gaacgcaatc
    24241 acttgaaaat taatgacttt gatctatgat atatagaaat cctatcgctt cgataggatt
    24301 tctatatttt acaaatggta tggatagcgt tatactaaag atatcttata caaagaggta
    24361 ttcatatgtc tatttataac aacattactg aattaatcgg tcaaacaccg attgttaaac
    24421 ttaacaacat cgtgccagaa ggtgctgcag acgtctatat aaagcttgaa gcatttaatc
    24481 ctggttcatc tgtaaaagac cgtattgccc ttagcatgat tgaaaaagct gaacaagatg
    24541 gtattctgaa acctggttct actattgttg aagcaacaag tggaaacact ggtattggac
    24601 tttcatgggt aggtgctgct aaagggtata aagtcgtcat cgttatgcct gaaactatga
    24661 gtgtagaacg acgtaaaatt atccaagctt atggtgctga actcgtccta actcctggta
    24721 gcgagggaat gaaaggtgct attgctaagg ctcaagaaat cgctgctgaa cgtgatggtt
    24781 tccttcctct tcaatttgac aatccagcta atccagaagt acacgaaaga acaacaggag
    24841 ctgagatact agctgctttc ggtaaagatg gattagatgc ctttgttgct ggagtaggta
    24901 ccggtggaac gatttctggt gtttctcatg cactcaaatc agaaaattct aacattcaag
    24961 tttttgcagt agaagcagat gaatctgcta ttctatctgg tgaaaaacct ggtcctcaca
    25021 aaattcaagg tatctcagct ggatttattc ctgatacact tgatactaaa gcctataatg
    25081 gtatcgttcg tgtaacatca gatgacgctc ttgcactcgg acgtgaaatt ggtggaaaag
    25141 aaggcttcct tgtagggatt tcctcagctg cagctatcta cggagccatc gaggttgcca
    25201 aaaaattagg tacaggtaaa aaagtccttg ccctagcacc agataacggt gaacgttatc
    25261 tctctacagc actttatgaa ttgtaaccgt ccaataacga agtatattga aaaatctcca
    25321 gactagagaa ctcacggata gttcctaatc tggagatttc ttatttgcac ttttcttgta
    25381 caattttagt ccatggtaaa taagcctcta aaacctcttt gtttacgaga gtttccacgt
    25441 ttggaagaca ttctagaaga taggatagat atttctcact atttataatg gattgaaata
    25501 agatatgaac aaatcgatta ggacatgatg gtaaagcgta atcccttgtt tctcagcttt
    25561 cccagacaaa aaagtccaat agtaagtcag ctgactatca ctctctagca ccctataaga
    25621 agtttcatcc gcatgaagta agggctgagt caatagtctc tctcgcaaga ggttatagag
    25681 gggctccaaa tagtattgac tcgtcttgat atgccaatta gagatttcct tgcgtgtgat
    25741 tggtaaaccc atcttagccc aatcttcttc ttggcgataa ttgggtacct tcagattaaa
    25801 cttctgatgg atggtgtgag cgataataga agctgagcca aagctatgcg ctaaaggggc
    25861 tttaggaata ggagctttca caagcttatc cagatgatta tcttttactc gttatggaca
    25921 atgctatatg gcataaatca agtaccttaa agattccgac taatattggc tttgcattta
    25981 ttcctccata cacaccagag atgaacccca ttgaacaagt atggaaagag attcgtaaac
    26041 gtggatttaa gaataaagcc tttcgaactt tggaagatgt catacaagga ctggagaagg
    26101 aggtgataaa gtccatcgtt aatcggagat ggactagaat gctttttgaa agcagatgag
    26161 tgtaaaaaga aagtcctcat ttcaatagaa atcacaactt tctgatggat ttatagtaaa
    26221 atgaaataag aacaggacaa atcgatcagg acaatcaaat cgatttctaa caatgtttta
    26281 gaagcagagg tgtactattc tagtttcaat ctactatatt tttggagtga tagaaaagcc
    26341 cttcataagc caatctactt gttcaggtgc gagagctttg acatcctttt ctgtacttag
    26401 ccaagtcagt tttccgttct caaagcgttt atatagtagc caaaatcctt gaccatccca
    26461 gtaaagggct ttaaagcggt ctttacgttc accacaaaag agaaagactt gaccggagaa
    26521 agaatccaat tcaaagtggg ttttaactac ataggctaat gagtctattc cctacctcat
    26581 atctgtcttg tcacaaacaa ggtgaacttg acctaaatca cttagttgaa ttatcatagt
    26641 acaatacctt tcctccgata attatttttt atctagtata ctggaagttg gggaattagg
    26701 atagatacct tgttatgacg cgcttactat gaatttgaag tatagtctcc taaatgcact
    26761 tagcccttat tatagggctt tttgttttaa ttattctaat cgagtgaggc tggggaaaaa
    26821 acaatttcag gaaaaatcta agccctatac aaaaaaggaa gcaatttgct tcctttctat
    26881 tattagttat tcaaggctgc tgccattgta gctgcaactt cagcttcgaa gtcgtttgca
    26941 gctttctcga taccttcacc aacttcaaag cgagcaaact caactaccga agcgttaact
    27001 gattcaaggt atgcttcaac tgtcttgctg tcatccatga tgtaaacttg tgcaagaagt
    27061 gtgtaagctt ggtcaacttt agtgttatca agcatgaagc gatccatttt acctggaata
    27121 attttgtccc agattttttc tggtttacct tctgcagcca attcagcttt gatgtcagct
    27181 tcagcttgag caataacatc atcagttaat tgagcttttg atccatactt caagtgtgga
    27241 agagctggtt tattaaccat tgcacggctt tcgttgtctt ggtcgataac gtgattcaat
    27301 tgtgccaact catctttaac gaattgctca tccaattctt tgtaagaaag aactgttggt
    27361 ttcatcgctg cgatgtgcat tgacaattgt ttagcaagtg cttcgtctcc accttcaaca
    27421 actgaaataa caccgatacg tccaccgtta tgttggtatg ctccaaagtg ttgtgcgtct
    27481 gttttttcaa tcaatgcaaa gcgacggaat gagattttct ctccgatagt tgctgttgca
    27541 gatacgtatg cagcttcaag agtttcacct gaaggcatta tcaaagcaag agcttcttcg
    27601 ttgttagcag gttttccttc agcaatgact ttagctgtag tatttaccaa ttcaacgaat
    27661 tgagcgtttt ttgcaacgaa gtcagtttca gcgtttactt caataactgc tgcaacatta
    27721 ccgttaacat aaacaccagt caaaccttct gcagcaacac ggtcagcttt cttagctgcc
    27781 ttagccatac ctttttcacg aagcaattca atcgcttttt cgatgtcacc gtctgtttct
    27841 acaagcgctt ttttagcgtc cataacaccg gcaccagatt tttcacgcaa ctcttttaca
    27901 agtttagctg taatttctgc cattttaatt ctcctatatt ttttgaaaat aggagagccg
    27961 ggctaagccc cgccctccta ggtaattact atttgtataa attaagcgtt gtcgccttct
    28021 acaacttcaa cgatttcttc aattgaatct gcttgagttt ctgaagctgc aaattctgct
    28081 tcaactgcta ctgcatcctc accttgacgt ccttcgataa tagcgtcagc caatttagct
    28141 gtgatcaatt taacagcacg gatagcgtca tcgttagctg ggatgattac atcgatatca
    28201 tctggatcag tattggtgtc aaccatcgct acaactggga ttcccaattt tttagcttct
    28261 ttaacagcga tttgctcttt atgtgggtca actacgtaca tcacatctgg gatacgaggc
    28321 atatcttcga taccgcccaa gaatttttca agacgcgcac gttgtttgtt aagaagtgca
    28381 acttctttct taggaagaac ttcgaaagtt ccatcttctt ccatacgttt aatttctttc
    28441 aaacgagcga tacgtttttg gattgttccc cagtttgtaa gagttccacc caaccaacgg
    28501 tggttgatga agtattgacc tgaacgtact gcttcttcag caactgcatc agctgcttgt
    28561 ttcttagtac caacgaacaa tacaactgca tcgttagctg ctgcatcacg catgaagtcg
    28621 tatgcttggt cagcgtattt tacagtttgt tgcaagtcga taacgtggat tccgttacgt
    28681 tcagtaaaga tgtacttagc catcttagga ttccagcgac gagtttggtg accaaagtgt
    28741 acaccagcct caagaagttg tttcattgaa attactgcca tgagtatttc tcctttttgt
    28801 ttttttcctc ttcttgactt caactcgcaa aacgacccaa gggcaactgc ttcacaattc
    28861 atcaagaatg agtattatcg ttcacacgac aattttcatt ctaccatact tttccaatat
    28921 tttcaagcta ttttgatata atttttaata gggattgtca cggtaaaacc tgaaaagagg
    28981 gctctattcg agtccctctg taaattaatc tgcataaata tatgtaacaa aaccttcaga
    29041 agttgttgtt ggattgaacc atccacggtg atttccaatt gtacgattac ctgcataatt
    29101 tgattctgat acttggatac gtgttgttga ttcaacagct gtaacaaccg ctacgtgacc
    29161 atatccacca tcattccaac atgcaattgc tccaacttga ggtgttgaac ctgtacggaa
    29221 acctgctgct gctgcacttg tagcccactg tgctccatta ccccagtagt ctccagccca
    29281 aggtgccaat gtttttactc cccatgtaca ttctccaatt ggataacttg aagcgtttgt
    29341 actgtatgtt ggacgaactt ttgcacggac aggtgctgct gcagattcag atactgcttg
    29401 cacttgagct gttaagttag tgtttgctga agcaagtact gattgttgtt ggctagctcg
    29461 tttttcttta taagctgctt ctgctaccgc agctgcacga gcctctgcct cagctgctgc
    29521 tttttgctct aatagacttg ctttttcccc ttcagctgtc gctttctcag cagcaagact
    29581 taattcagca gcttttagtt ctgcctgttt cgtagtcaat gcttgagcat catcagccaa
    29641 tttttgttga ttagcaatta cagtattgat agcatcatta tttgctactt gtttttcaga
    29701 aatagctttt ttatctgcct tttgttgttc taacattttg ttgtttgcag atacgatttc
    29761 actcattgca gcaacacgtg aaatagcttc tgtaattgat tttgagttta caatggtatt
    29821 gatatagcta gttacggctc catttgtttg agcactacga gcttgttttt ccaacgattg
    29881 gttacgagaa acaatgtttt tagaaagttc tgtaatctca ccctcgagtt tcttagattc
    29941 tgcttgtaat ctatcatttt cagcttgcaa gttagactgc tcagcttgaa tagctgatac
    30001 ttgctcctga atttggtcaa cttgtttttg ggcttcttgt tgttgtgctg ttaagttact
    30061 aattttatta tcttgagcag caattttgtc atcagtcgtt tctgcatgcg cagttgttaa
    30121 aacagctact tgagaaacca ttactgtact taataaaagt gacgctaaga tttttttctt
    30181 catattacgt agatactcct tcttttaaag acaatactag tataccaaaa aaaggcctaa
    30241 taaatattac gcctttgtta catttatatg tcgttcttat agataatatt tttcaaaaat
    30301 aaattgaaaa acagtaatcc ataaaaaatt taaaatcatc gtcggtacca agctatagac
    30361 tataaaaatc gacatgctat ctactgtcaa ccctaccata aaagccaaaa gataactacc
    30421 catttcaaac aggaatgtca gcacaatcat agctagcatt cttgtccaac gattcaacaa
    30481 aataacacta ttcaatttat gaaggaaggc tcccaataag ataaataaga gagttgtaat
    30541 ccctattaga tggaaaaagt aaacatcata aaccaagcct ataacaaaac aatagactag
    30601 gtagagatac tctgatactt ctatcgtctc aaataagaga aatagaaaaa gaaaatgact
    30661 agccaaatgt acatggggga aaaatgagcc cagaagctgg ctaatatggg cgtcaattag
    30721 aacaaagaaa ggaagcaata aaaatactcc aactcgcttc aactgtctca ttatgaattc
    30781 cccactaatt ctatcacatc tacattatga gtatctgcac tcaatttaac agttacttct
    30841 cgtgtcaaat agtctgtact atgcgttgtg gcaaccactt caccaacagg aatatcagca
    30901 acgttaaagt ttcctaatcc accagtagtc accttatctc ccgcactaat atcgctatta
    30961 ctatttaatt ggctaatttt aagaacgtca ttttccttgt catagccaat aataattcca
    31021 taaattgtag tagagccatg ttgaatttta acagaaatct tatcagcatt ttccgtattt
    31081 gtcagaaggt tgactatagt agagttctcc tctacttttg aaacactccc aatcaagcca
    31141 ccatttgcaa tagctaacat gttctcagaa gcaccttttg atctacctgc atctaaggtc
    31201 aactcctgct tccaagatac cggagaacgc ataataacat ctgctgctaa agtctttgtg
    31261 gcttgcaatt tagacttcat atcaagcaat tggcgcagtt gttcattttc tgtctttaaa
    31321 ctttccacct catttgattt aacttctaat tggtaaagct gtttcttcaa actttcattt
    31381 tcattatatg ttcgtgtcaa atgagccaaa tctgatttga cagaatcaaa ccactgaaaa
    31441 ggtttttgta caactctatc aaccaatgag attccatctc ctaattttgt cacaattgta
    31501 cttgaataag tcgtcgctaa gagagctgac acaagcagaa cagtgacaaa aacaataatg
    31561 acatattttg attttttaaa acggttcata ttcctacctt tatatcaaaa actgttacag
    31621 taacttttta tcaattcctg aaagctacta agattttaag aaaaataaac aacaaccaag
    31681 tacgagaata acaagaatgg tcagcgtatc cttcagagtc catttcaatt gtcggtattg
    31741 acttctgcct tttccaccct gatagccacg cgcttccata gcgatagcca aggaatctgc
    31801 acgttttaaa cttgtcgcaa aaagaggaat caaaatggga atcatcgcct ttactttttg
    31861 aacgatgctt ccttctccaa aatccactcc acgagctttc tgtgcattca taatccgagt
    31921 cgtatcatcc atcaaggttg ggacaaaacg caaactcata gacagcatca atccaatttc
    31981 atgaactgga actttcacac gctttaaagg tgctaataaa gcttcgacag ctgatgccaa
    32041 acttaaaggc atggttgtta aggttaacaa agttgaaaag aaaataatca atacaaagcg
    32101 acaaaaaata atcccagctt gttgcaaagc ataatccgtg attctcacaa acgaaaactc
    32161 aaataaaaca ttcccattag aaatgaaaaa tagttgaaaa atagttgtga aggcaatcaa
    32221 gaaaaacata gacttcaagc cctgaataaa aaatgaaaga gatactcctg acaaggcaat
    32281 aaatatccct gtcgctataa aaagaatcag attcgtcaag gggttattag cccaaaacac
    32341 aatcaaaatc agtagcatca tagcaagcaa tttgctacgt ggatccaatc ggtgaacaat
    32401 cgaatcccct gggatataac gccctaaaat catactatcc atttagcgac tccttgaact
    32461 cctctatctt aatcggtaat cgtttaaatg acacgcctct atcagccaat cgtttacaaa
    32521 aggccgtaat tttaggtact cccaactgaa cttcttccat aaaaacaacg tcttgaaaga
    32581 catcacttgg tttgcccccc tttactaaac gtcccttttc cattacatag acttgattcg
    32641 catattcagc aacatcatcc atcaaatgcg ttaccaagac gatggtcatc cctgactggt
    32701 ggagtttttt gaacagggtc atcaactctt ttctccctag aggatctaga ccagctgttg
    32761 gctcatctaa gactaatata gctggctcca tggcaagtat gcctgcaatg gcaacacgtc
    32821 tcatttgtcc ccctgacagc tcaaacggac tacgatcaaa aagtgattca tcaattccaa
    32881 ccagagccag tttctcacgc gcagtcttca cagcatcttc ttcagaaact ccaaaatttt
    32941 gcggtccaaa agcaacgtcc ttcaaaaccg tttcttcaaa aatctgattt tcagcaaact
    33001 gaaataccaa gccaacctgt tttctaattt gacgaatatc tttattttta gaagtcgagg
    33061 tgattaaggt atcaaaaacc ctcacactcc cttgacttgg caccaataaa ccatttaaga
    33121 gttgtaaaat agttgattta ccactacctg tgtgcccaat taaagctgta taagagccat
    33181 cttcaatcgt caaagaaaca tccgacaaag ctgctgaagc taagggagta ccttcttgat
    33241 atgtaaaatt cacattttct agagcaattc ccatagctta tcctctagct cactttctgt
    33301 caaataattt tcaggtaaat catagccatt ctggctcaaa gattttttta attgattagc
    33361 aaaaggatcg tctaatccaa tttgatctaa atcatttcga gagaaaagct cccttggact
    33421 actagttgat tcaatttccc cttttttcat gaccaatacg cgatcactca tggcgacttc
    33481 ttccaaatca tgggtaatag aaatgaccgt catatcatag tcttttcgaa ttccttttac
    33541 tgtcccaata agttctctac gcccctcagg atccaacata ctcgttgctt catctaagat
    33601 taaaatagct ggtcttaggg ctacaacacc tgcaatggcc acacgttgct tttggccacc
    33661 tgatagacgc gctggctctc tctttttaaa gtccaacatg ccaactaaag ccagagcttc
    33721 ttccactctc tttttcattt cttgacgaga aagtccctga ttttccaaac caaaagcaac
    33781 atcatcttca acagtcgctc caacaaattg attgtctgga ttttgaaaaa ccataccgat
    33841 ttgacgacgt atattccaaa cattttcctc agtcaaacgt tggccatcaa ttacaatctc
    33901 tccggattct gcttccagta agccatcaat taatcgaacc gtcgttgatt taccactacc
    33961 attatgccct acaatcgaaa gccattctcc acgtttcacg tgaaacgtaa tatccttcac
    34021 atcgtagtag ttctgatttt ctttatagcg aaaagaaaga ttttttacat caattattga
    34081 tttcatttcg aaccaaatgt ccctttaaat acataggcac tacccttgaa atagtcatag
    34141 ccagagtaga tagtgaaaaa taaggctaca taaagtagaa cttgaccaag caaagtccaa
    34201 tgtaatagca agaaaataat ggcaaacatc tgactaaaag ttttaatttt tccaggcatt
    34261 gctgctgcta aaattgttcc accagtttca accaataaaa gccttaaacc tgtcacagct
    34321 aactcacgac agataatcac tgcaacaatc caagccggag ccatacctaa ctcaatcaac
    34381 ataataaaag ccgacataac tagtaactta tccgccatag gatctgcaaa tttaccaaaa
    34441 ttactgacca cattccattt acgagctaaa tatccatcta aatagtcggt aatactggca
    34501 acagcaaaga taatagctgc aactatatga ctctctatcg aatttcctat cgttaaaata
    34561 aagataaaaa taggtataaa gagaattcga cctattgtta agagattggg aatttgttct
    34621 tttttcattc gtttttcctt aatttttagt aaaggttaca gtgatttgtc cagtctgagc
    34681 tgttaatttc gataaatcaa cagtctgatt atctactgtc aaatcaacac cttttacaac
    34741 acctaacgta attgttacag gacttttagt tgcaactgtt gcttctgcac ttttcttctt
    34801 tggcgataag gttacaccgc cctcaagttc gctttctgaa acactgaccc aacttgtaac
    34861 atctgaaact gccaattgca atttaactgt ttccttactt gtcttataag cgatttctac
    34921 atgatttcct tcgcctgata cacttatagc tgattctata ctagaagaac tactacttga
    34981 ggagtgggga acagagctag ttgaacttgt tgattgaacc acactgtaat tagaaagaga
    35041 aggctcctct ggttgagttt gaatatagtt ccaaacataa taagtcacaa aaattaaaat
    35101 cgataaagca aaaaagataa aataaaataa aggtaaaaat gatatttttt tcttcttttt
    35161 cttacttgaa cgtctacgac ctgtcaactc atcttcatca acatctactt cctcataagt
    35221 aatcatactc ccagaatcat aagcatccaa aacaatttgg tcatctaact caacagccca
    35281 tgcatatttt ttcaagaaag aacgcgtgta aaaaggactt ggaagttgat cgaaatcgtc
    35341 tgcttccatt gcttccaaca tatctaactg gatttctgtc tttttctgca attcatctaa
    35401 actcaatccc tgattgattc tagctaatcg taaaacctct ccaattgttt tttttctcat
    35461 acttgtcatc ccttctttct agtgtctatt ataataggtt actacgatgg gaatattgta
    35521 aaatcaacta tgtcaccatc atctattaaa tgatgtccag catcaaggac atcctctaaa
    35581 gtaatttcct gtaaaatttt cggcaaatca aaaattgtct caccattttc aaaagcatca
    35641 tattgcgttg caataaattc aagagagttc atgctactga aaaattcgcc aaacatctct
    35701 cttttgataa tatctaaatg ttcctctgta atatctaaat cctttgtaaa attacgaata
    35761 gccttcctaa attgatgaga caaagcaact ggctctttcg tatccattgt caacatgaca
    35821 aaatgaaagc gacttgttat ttcaacttcc agagataagg acgcatcaat tttacctgat
    35881 tcataacatt tttgaaaacg atccgaagtc caaccaaaca tcattgcaaa caataatttt
    35941 aataaaatat gatgtcgata gcaatccgct tcagaaactt ctcgcttacc tctaactcca
    36001 atcgctagtt tgggagaaga tacttccatt ctcatactat ctgttggctt tacaggctgt
    36061 aaaaacaact tttctcttgc tacttcctga aaatctgaat ctttcagttc tttgctttca
    36121 aaatagtcct gtactcgctc cacatcaaaa ttaccaacta aaaacagaga catgtttaca
    36181 ggtttgtaaa actttgtaaa attttcttgc aaattagtta gattgatttg ggaaatggac
    36241 tcctcacttc caactatatc agttgctaaa ggtgtaccag gatacaaatt cgctaaagtt
    36301 gaaaagaata aacacgaatc tggatcatct tggtacattt ctcgttcttg ctgaataata
    36361 tcctgctctg tcagaatgga agcttcagta aagtgtgctg atgttaccaa ttcatcaagt
    36421 aaatataaat tttctaaaaa ataatccgtt gctgaaaaaa gatagtttgt ttttgtaaag
    36481 cttgtaaagg cattactatc tgcacctaga ctcgtaaaag ccgacatcaa atcactagaa
    36541 tcttctctct caaataattt atgttcaaga aaatgagcaa ttcctccagg atattgtttt
    36601 acatctccgt caacttctgt gacaagcgta tctatcgaac caaactgtac agttacactc
    36661 ccgtaaacct ctttaaattc ctttttaggc aaaagagcaa ctgtcaatcc gttggccaaa
    36721 cgagttcgat aaaccttttc ttttacagct ggatagtatt tttcttcaaa aacaaccttt
    36781 gtcattctat tccttccata aagtaaatcg cttgtagttt cacattatta gctactctac
    36841 aaatagcatc tttgtcaatc tgttcaagct ttgcaatcca acttttaaag tctgctgaag
    36901 attttccaaa taaggcattt tgataagcac gttcaatcaa tgaagattga ttatcttgag
    36961 aaagtaacaa cgaccaacga atcatttcct tggtctgatt taactcaaac tctgtaaaat
    37021 aacctttttt taaatcaagc agttgattat tcatcatttt acgagcctgg ttacgatttt
    37081 ctcgattgat accagcatac atcctcaaga atccactaaa taaatcaagc tcacttgaaa
    37141 tggtataagc taatccagca ttttcacgga catttgtaaa gagcttagag tgagcaaatc
    37201 caccaagtaa accattcatt acaatcatgg gtaaatgttg ctcatcacca tatttagaac
    37261 agtaatgata acctaattcc aaaatggatt gtcccacatt tttccgaacc ataccttcct
    37321 gaaggatatt agaataaggt tgacaatact gaaccttcac atctcctttt cgacctttaa
    37381 agccaaatga ttctaataca ttttgaattt caacctcatt aaaatcacct aggaaaaaga
    37441 aatctattcg atcattggct aaaaattctt ggaaacaaga ataagaactt tgtggagttt
    37501 cagctaaaat acgatttcgt aaatcactat attccaattg aagacgttca tcatgaaaaa
    37561 acaatttatc caattcttta tgtgcaaaat aaaaagaatc atccatatca gctgctaaac
    37621 ttgctagcaa ttgttttttc tcaatttcaa ataaggccgg atcaaaccca ttatcaacta
    37681 ctacgggtga aaaaagagtt tcttttacaa gttccaaaat ctgagaggtt agcacgtttt
    37741 tcctacttaa aaactcatca cgaacatagg taaatgtcaa ttctataatg tggctttgcc
    37801 ctcttctgaa acaattggtt gacatatctg taccgtatag actggccaag tgtctcctca
    37861 aatcttgaga agtggggtac atctgattag cagtctctag catacttgca ctcaacatgt
    37921 gacctgcaat cgtatcaagg gataatggag cggtaaaacg cacggtaatt ttgtttgttt
    37981 taaacttttt tgattggata aaatgtgttg aaattccatg cactaactcc atgatttacc
    38041 tccttatata cagacttcta ttatatcacg aaaacctgaa attttcttgt tccctgtaac
    38101 acttttgaaa taaaaatcgc ttacatttct cgctgtctct ctcactattt ttaggaaaat
    38161 atggtataat accaaagatt gaggtgaatt atggaataca aattatttga agaatttatt
    38221 accctccaag cactactcaa agaacttgga attacacata gcggaggagc tattaaatca
    38281 tttctctctg aacattctgt ttactttaat agggaattag agagtcgtcg tggaaaaaaa
    38341 cttcgtattg gcgataaagt tgacatccct gacatgaaca ttgacatctt gttgacacaa
    38401 cctacttctg aagaacaaga tgaataccaa gctgataaag ttgaaaaaga acggatcgct
    38461 aaacttgtca aaaagatgaa taagggagtt aaaaaagaca aatcgaaacc tgcttcatca
    38521 cctaaaagcc aacaagctcc acgattccct ggtagataat catgtggcta caacatctat
    38581 ctctcaagac ttttcgtaat tacaaagaaa cgaaaataga ctttaatcct aaattgaatg
    38641 tctttttggg acgtaatgca caaggaaaaa caaacatgtt agaggctatc tattttttag
    38701 ccttaacgcg tagtcatcga actcgaacag ataaaaatct cattcatttt gatgaggaac
    38761 aacttcatct ttcaggtctc gttcagaaaa aaactggatc cattccccta gaaatcgaac
    38821 taacacaaaa aggccgtgta acaaaagtta atcacttaaa acaggcacgc ctttcagatt
    38881 atgtaggaca catgaatgtt gtcttatttg ctcctgaaga tttacaacta attaaaggag
    38941 caccttcgat tcgacgaaaa ttcattgata tggaacttgg gcaaattaag ccaatctact
    39001 tatctgactt aaccaattat aaccacatcc taaagcaaag aaacacttac ctaaaatcag
    39061 atcaaaaaat agatgaaaca ttcctttctg tgttagatga tcagctagtt gattatggat
    39121 gtcgtgtaat gaatcaccgc ttagatttca taaaaaaact agaatcattt gggcgtaaga
    39181 aacattttga actctctaat cagatcgaag agttgtcaat atcctatcaa tcttctgtca
    39241 atataactga caaacaaaac ttatccgaat ctttcaaaat tgctttagaa aaaagtagat
    39301 ccagagattt atttaaaaag aatactggtg tcggtcctca tcgagatgac atttcttttt
    39361 atataaatgg gatggatgct agtttcggaa gccaaggtca acatcgtagt ctcgtcctct
    39421 cgataaaatt agcagaaatc gaattaatgg aaagcattac cacagaatct ccgatattac
    39481 tgcttgacga tgtgatgagt gaacttgaca acactagaca gttaaaatta ttagaaacga
    39541 tttctcaatc aatccaaacc tttatcacaa caacaagctt agaccatctt caaaatctgc
    39601 cagaaaatct aagtatcttc actattcagg atggtaaagc ttctgtaaat ggaaattgac
    39661 aacattgtaa aagaactcct gttttcactg gagttctttt cattgttttt tacatagaat
    39721 aatttggtgc ctcattagta atttgcacat catgaggatg gctttctttc aaaccagcac
    39781 cagacatttc aataaattga gcattatcgt gtagttcttt aaggttagct gcaccacagt
    39841 aacccatacc agagcgaata ccaccaatca tttggaaaac aatatcagct gccgctcctt
    39901 tataagcaac acgaccttca attccttctg gaacaagctt gtttgcttca ttgacagaac
    39961 cttggaaata acggtcgctt gaacctttct tcatagcagc aattgatccc ataccacggt
    40021 aagtcttgaa tttacgtcct tggaagattt cagtttcgcc tggagcttca tcagttccag
    40081 caaacataga tccaagcata acagcatttc cacctgcagc aagtgctttt acaatatctc
    40141 cagaatactt gatcccaccg tcagcaataa tcgttttacc atattcgcgc gcaacagctg
    40201 cagcatcgta gatagctgtt acttgcggaa caccaacacc agcaatcaca cgagtagtac
    40261 agatagaacc tggtccaata ccaaccttaa caacgtctac acccgcttca taaagggcac
    40321 gtgcaccttc agcagtagca atatttccag caatcaaagt ccgatctggg aaatgagcac
    40381 gaatctcggc aattttacgc aagacacctg cagaatgacc atgtgcagta tcaataacaa
    40441 tcgcatccgc tcctgcctca aaaagagcct ctgcacgttc aaatgtatct gaagtaacac
    40501 ctactgcacc tgcaactaga agacgaccaa actcatcttt agccgcattt ggaaactcaa
    40561 taactttttc aatatctttg atagtgatca aaccagaaag actgccttct tcatcgacca
    40621 acggaagttt ttcaatacga tgctcttgaa gaatactctc agccgttgca agatccgtac
    40681 ccacaggagc agtaacaaga ttttcactag tcatatggtt tgaaattggt tgattataat
    40741 ctgaaataaa acgaagatct cggtttgtca aaataccaac caatttacga ttttcaagtg
    40801 tttcaacaac tggaacacca ctgatgcggt aacgacccat aagctcatct gcttcagcaa
    40861 ttgtatgttc aggcgtcaag aagaacggat caataataac tccattttca gaacgtttta
    40921 ccttacgaac ctcgtctgct tgttgagcaa ttgacatgtt tttatggata actccgagac
    40981 cgcctgcacg agcaatagca atggccattt gactctctgt aactgtgtcc atggcagcgg
    41041 taataattgg gatatttaaa gtcaaattat ctgccaattt agttgttaaa tctgcatcgt
    41101 taggcaacac atgactttca gctggaataa gcaatacatc atcgaaggta aaaccttttt
    41161 tcaaaaattt agtgtcccaa ttagacattc gaagtttcct cttttctttc tttttgagct
    41221 tgactctttt tatattgtat ctatcatacc attctataaa aatttgtcaa atgttacagg
    41281 ggtaaataaa aaactatctt ttcttcgaga tagaaaagat agtttcttgt aaaataaact
    41341 tagttaaagt aatgaagtcc cattgcccct tttacctcag atagggtttg acccgcaact
    41401 tcacgcgctc tttcactacc tttttgaatc atattataaa cttctcccat atccttagca
    41461 aattcaatac ggcgctcacg aataggaccc agttcacgtt ctaatatttc aagtagataa
    41521 cgcttggtct tcacatcacc aagaccacct cgttgataac gttctttcat atcagcaatt
    41581 tcttgagcat cttctggacg accaaaaaca tctagataat ggaaaaccat atttccctca
    41641 atcttacctg gatcctcaac gcggatatga tctggatctg tatacatact cattactttt
    41701 ttacgcaaag tatccgcatc atcagctaaa taaataccat tatttagtga tttagacatt
    41761 ttagcatttc catctaaacc aggcaaacgc cctgctctct cattttctgg ataaatacct
    41821 tccggctcta ccaagacatc acagttatat gcattgttaa aagaacgaac aatttcacga
    41881 gtttgctcaa tcattggttt ctgatctgtc ccaacaggaa cataattagc cttgaaagct
    41941 gtgatatcag ctgcttgagc gattggatag accaagaatc ctgtcggaat gctttctcca
    42001 aatcctttct gagaaatctc tgtcttgact gttggatttc gctccaaacg tgctaacgaa
    42061 actagattca tataatacat agacaactca gccaactctg gaatctggct ttgaataaaa
    42121 atagttgact tatttggatc caatccaact gcaagataat ccaaagccac atttccgata
    42181 gactctacaa tggtttgagg atctttggca tgatctgtca aggcttgttg gtcagccaag
    42241 aacacaaaca tatcatactt atcctcttcc tgtaataata ctcgattttt gagacttcca
    42301 acataatgtc caatatgcaa ttttcctgtt ggacggtctc ctgttaaaat aatgggttta
    42361 gtcattttgt tctccttcga tatagtcctt tcaattatag cattttttta atgaaataac
    42421 cgtaatttat caaaataaat cagccttgct ttttacagat ttgtgtaaag gatactgtaa
    42481 atataattta cacaaaattg aaaaataaaa atttgaatat ttctgtattt tctagtagaa
    42541 taaatatatt acacatatag gagaaaaata ttgcttacag tatctgatgt ttcactacgt
    42601 tttagtgatc gcaaactttt tgatgatgtc aatatcaaat ttacagaagg aaatacttac
    42661 ggattaatcg gtgctaatgg tgccggaaaa tcaacctttt taaaaatttt agctggagat
    42721 atcgaaccta ctactggtca catctctctt ggtccagatg aacgtctctc tgttcttcgt
    42781 caaaatcact ttgactacga agatgaacgt gccattgatg tcgttatcat gggaaatgaa
    42841 aaactttata gcatcatgaa agagaaagat gctatctaca tgaaggaaga tttctcagac
    42901 gaggacgggg ttcgtgctgc cgaactcgaa ggagagtttg ccgaacttgg aggttgggaa
    42961 gcagagagtg aagcctctca actacttcaa aacctaaaca ttccagaaga attacactac
    43021 caaaacatga gcgaattggc caacggtgaa aaagtaaagg ttctcctcgc caaagcactt
    43081 tttggtaaac cagatgttct tctcttggac gagcctacta acggtttgga tatccaatcg
    43141 attacttggt tagaagactt cttaattgac tttgataaca cagttatcgt agtatcccac
    43201 gaccgtcact tcttaaacaa agtttgtact cacatggccg accttgactt tggaaaaatc
    43261 aaactctatg tcggaaacta cgacttctgg aaggaatctt ctgagcttgc tgctaaattg
    43321 ctagcagacc gtaatgctaa agcagaagaa aaaattaaac aattgcaaga attcgttgct
    43381 cgtttctctg ccaatgcttc taagtcaagg caggcaacat cacgtaagaa aatgcttgat
    43441 aagattgagc tagaagagat tgtaccatct agtcgtaaat atccatttat caactttaaa
    43501 gcggaacgtg agattggtaa tgatctcttg acagtagaaa atctaactgt aaagattgat
    43561 ggtgaaacta tcttggataa tattagtttc atcttgcgtc cagatgataa gacagcactt
    43621 attggacaaa atgatattca aacgactgca ttaattcgtg caatcatggg agatattgac
    43681 tatgaaggaa ctgtcaagtg gggagttaca actagtcaat cttacctacc aaaagataac
    43741 tcagctgatt ttgcaggagg agaatcaatt cttgactggt tgcgtcaatt cgcaagtaaa
    43801 gaagatgatg acaatacttt cctacgtggt ttcctcggcc gtatgctctt ctctggagat
    43861 gaagttaaca aacctgtaaa tgtcttgtca gggggagaaa aagttcgtgt catgctttca
    43921 aaactcatgc tcttaaaatc aaatgtcctt gtacttgatg atccaacaaa tcacttggac
    43981 ttggaatcta tctcaagctt gaatgatgga ttgaaaaact ttaaagaatc aatcatcttt
    44041 gccagccatg accacgagtt tattcaaact ttggctaacc atatcattgt cttatctaaa
    44101 aatggcgtca ttgaccgtat cgatgaaacc tacgatgaat tcctagaaaa tgcagaagta
    44161 caagcaaaag ttaaagaact ttggaaagac taaataaaac ttcaaaactc agttgggtta
    44221 accaactgag ttttctatca ttctacgagg taacatgaaa tcatttttaa aaacatatcg
    44281 aacctatttt atttctttca tcattcctgt agtgattatg tctggagtat atctatctca
    44341 aggtatctac tggaatagcg acaactctcc tctattagga gatggctttc atcaatacgt
    44401 tatttttgat gtagccttac gaaatatcct acatggaaat agtagtctgt tttacacctt
    44461 tacaagtggt ctagggctaa acttctatgc cctatctagt tattacttgg gtagttttct
    44521 cgcgcctcta gtttactttt ttgatctaac gaatatgcca gatgctgtct atctgaccac
    44581 tctcttaaaa tttggattga ttggtctgtc aacctttttt agtttgaata aattgtttca
    44641 atctatccct cagactttaa aactagcctt atctacttcc tatgctctga tgagtttcac
    44701 tgtcagtcaa ttagagataa aaacctggct agatgttttt atcttgattc ctttaattat
    44761 aactggttta catctactga taactgaaaa gaaactccta ttgtacttta caagtctgtc
    44821 aatcttattt attcaaaatt attattttgg atatatgaca gtattgtttc ttattttctg
    44881 gtatctctgt caaatttcgt gggactttaa gactcgaaaa tcatctgttc ttgatttcat
    44941 agttatctcc tttttagctg gtatggctag tttgattatg actcttccca ctctatttga
    45001 tttacagaca catggggaaa aattgactga agttacaaag tttcaaactg aaagtagctg
    45061 gtatcttgat ctctttgcta agcaattcat tggttccttt gacacaacaa agtatggggc
    45121 catcccaatg atttttgttg gactatttcc ctttattttg accattttat tttttacgct
    45181 gaaatctatt aagtttcacg tgaaactcat atatgtaata ttctttgcat ttctaattgc
    45241 aagcttttac atagaagctc ttgacttatt ttggcaaggc atgcatactc caaacatgtt
    45301 tttacatcgc tatgcttgga ttttctctac cttgttaatt tacacagcag cagaagtctt
    45361 aaagcgtctg aaagaactta aagtctggaa ttttttagtt tcgctttttc ttgtagtaac
    45421 aggattttta gctaccatct atctaaaatc gcattattct tttttaacag atttgaatat
    45481 tctgcttact cttgaatttt tggttgtcta ttctctttta ctccttgcag ttatcaaaaa
    45541 gtttatatct gtgaatctat ttgccattct aatctcttta tttatactgg ttgaaatgag
    45601 tttaaatgct tcatctcaaa tggacggaat tgctaaggaa tggggatttg cttctcgaag
    45661 tgcttatagt cgagatatcc cagctatgga atctttctca acatatattg gaaatcaatt
    45721 tactcgtact gaaaaactac aaactcagac aggaaatgac agtatgaaat tcaactacaa
    45781 tggaatctct caattttcat ctgttcgaaa tcgttcatca agctctactt tagataaact
    45841 tggttttaaa tcctctggga ctaatctcaa tctccgatat gcaaataata gtattttggc
    45901 tgatagttta tttggtatcc agtacaatat ctcagacagt cctattgata agtatggctt
    45961 taaagatatc tatcaaaaag ataatcttac cctatatgaa aatcaatact ctcttccgat
    46021 tgcagttgct agtcaatctg tttacaatga tgtcaagttc aatgaacata ccttggataa
    46081 tcaggcctca tttttaaatc aacttgctaa cgtcaatttt gattattttt ctccaatacc
    46141 ttatgaaaaa acagaaaaaa tagaaaatac taatgatttg attagtgtca caagttcttc
    46201 aaatgaagat gcagcaatcc agtatcaaat tgaagttcca gaaaacagcc aagtttatct
    46261 ctctttcata aaccttcact tttctaacga taaacaaaag aaggttgaca tccttgtaaa
    46321 tggtgaaaaa aagactttta caactgataa tgtcttctcc ttctttaatc taggatatac
    46381 taaagagaaa aaaactttca atatcaatgt tagtttccct ggaaattcac aagtatcatt
    46441 tgaatctcct accttctatc gtttagatac caaaactttc accgaggcaa ttcaaaaaat
    46501 taaagaacaa cctgtcacag tatcaacttc taaaaacaag gtttttgcta catatgatgt
    46561 ccaacaagat acatctattt tcttcaccat tccttatgac aaaggttggt ctgcctacca
    46621 agatggtaag aaaatagaaa ttaaacaagc tcaaactgga tttatgaaag ttgacattcc
    46681 caaggggaaa ggaactatta cactttcctt cattcccaat ggttttatta ctggagcaat
    46741 ctgttccttt acttctctct tactatttgg aatctataat cacagacgaa agtcatctaa
    46801 ggcataaaaa atcgaccagt taatcggtcg atttttttaa tcttcttcca ttttcttatg
    46861 ttgataggct agcataccta aactagtaaa tagaactagt atgacagcta ggaaaatgac
    46921 ttgttgatag agattgtttg tcgtaatccc gaaactgaat agctcattgg taaccaggga
    46981 ttaatagatc taaagaaatc atttgtcaaa gcaagtggat aagtacctgc acttgatgct
    47041 aactgtagta aaagcaaaat aagtgagaaa aaagctccta tacggctatt ccacgttgct
    47101 aaagtggtca ccatagacat gaatactaaa cttgttagga tgatgagaat aaatattctc
    47161 atctcatgat tagcagttaa accaataagc tgaactcctc catataccaa aattcctgcc
    47221 aaaacagcta taataccatt tatttcagct caagatttca accaagccca acggctctct
    47281 ggatgacgtc ctgaaggcaa tttcgcaaat atcatatttg ttgatattgc tgcaacaaaa
    47341 agagcaactg atatcatata aggagctatt gcgattccat ttacaggaac ttgatcattg
    47401 tctgtttttg aaagattgag tggatttgac aaaatctctg catttttaga ttctgttgat
    47461 actgatttga gttgatcact agcattacct agtccttgtc ctaaagaagc aagtcctgtc
    47521 tgtaaatctt ccaatccaga agttaacttt gttccacctt ctgctagttt cccagatcca
    47581 tctgccaatt tattagctct attctctaat ggagaagcac ctgaaagtaa ctgactggat
    47641 ttgtctgcta attgcccaga tcctgattgt aattaaccaa ctcctgctgt caatctagaa
    47701 gatttttgtg tcaaggtgtt tgagcctgaa actagtttat ccaaactacc tgtcaaggtg
    47761 gcattttttt cacttagttg acttgcgccc tgagaaactt tatcaacacc tgtagtatat
    47821 gcgtttacac ctgatgcaat cgactgactg gcaggaacta atttgctagt aacatctcct
    47881 tgtatctctg ttaatccact tgacaatcct atcaaagaag tagaagtaat aggtgatact
    47941 tgattagatt gattttttaa agtcgaaaga ttagaagatt gattttgtaa gttttctaaa
    48001 cttccctgta aatcttgtac taaagctaca attgactgag ccgattgaat actatcagtt
    48061 gaattttgag atacagaatc acttatctca gtttgttgct cacttgtcaa tgattgataa
    48121 gctgctgtcg attgaatatt ggataatgta gttgtttttt cagataaatc acttggtaaa
    48181 gtcactacca tatagtaatc gccatcttcc aatcccttct ttccttcctc ttcatctaca
    48241 aaatgaaaat ccaaggtttt attttctttt aaattggaca ccatgtcttt tcctattgcc
    48301 atagtattac cattatagga agcctcttta tcattattta caactgccac aggtaagtca
    48361 gacaattgcc catatgaatc ccacattgat gacaaaaata tgatattgta cagatctgga
    48421 ataagagaaa tcccgatcat gacaataata aaggttggtt ttttttaaat tgctttccat
    48481 tctttaaact tagtttctcc ttttttaaac atattgtcta aaattttaat ataatagatt
    48541 atacattaaa aaatctaaat tacaagataa aaaatctatt tttagacata tagtctaatt
    48601 tgtttacaag gaggaaatat gcaagaaagt aacaaacgct taaaaacaaa gcgaactatt
    48661 gaaaatgcta tggtacaatt actgatggaa cagccatttg ataaaatttc tactgtcaaa
    48721 ttagtagaaa aagccggaat tagtcgttcc agcttctata ctcactataa ggataagtat
    48781 gatgtgattg agcactatca aagcaagcta ttccatacat ttgaatatat ttttcaaaaa
    48841 catgctcatc acaaaagaga cgctatttta gaagtctttg aatatctaga gtcagaacca
    48901 cttctggctg cccttctttc tgaaaatggg actaaagaaa tccaaaattt cttacgaaat
    48961 aaacttcata tcatgctcag tatagattta caaaagcgat ttatgcaact gaatctaaat
    49021 accactgaat tagaatatag tagcatctat ctaactcatg cactttttgg tgtctgccaa
    49081 acttggattg cacatggaaa aaaagaaagt tctcaagaaa taacagactt ccttatgaaa
    49141 atgcttggtg atacaaattg atacaaaaag aggaacacag ctgtgttccc ttttatctat
    49201 actccgccag taggactcga acctacgaca tcatgattaa cagtcatgcg ctactaccaa
    49261 ctgagctatg gcggataaaa tagtccgtac gggattcgaa cccgtgttac cgccgtgaaa
    49321 aggcggtgtc ttaacccctt gaccaacgga ccttctatct gtagcagata taaccattat
    49381 atcaatttct tgctaattgt caatcacttt tgagattttt tctctaaaat atcttttaat
    49441 tttctaattt ttaatcttga aataggacaa cgatggtctt catagaaaac aatttctaag
    49501 ttttttcgat caatttctct gatattgcct atatttacca aaaatgactt gtgaggagaa
    49561 taaaatcgct gagtatgttt gtccttttcc tgaatatctg tcatggtacc ataaaactct
    49621 tttgcaaaat tcttaccaat aatgcgcaat ttatgagata cccctgttgt ttcaatatac
    49681 aaaatatcat ggtaaggaat ttttaaatca tttcccttgt aattgtagtc gaaataatct
    49741 acaacatctt cattttcaag taacatactc ttcgtgtaga agatattttg ctcaattctc
    49801 ttcttaaaca tctcatcatt gatatcctta tcaacaaaat ctagggctga tacctggtat
    49861 ttataggtta gagtcgcaaa ctctgatcga ctagtgataa agacaataat agcgtaagga
    49921 ttgtaatgac gaatgagctg agccacttca aatccctttt tctcaattcc atgaatatcg
    49981 atatctagga aataaagctg atttacttca tcattttcaa tgtattcttc aaattcacgg
    50041 acttttcccg ttgtcttgta tgatattgga atattcgatt ctttcgaaat ttcatccaat
    50101 attctctcta gtctcacttg atgttcaata acatcttcta aaattaaaac tttcattcaa
    50161 attccctctt aaatctaatg atttgtctaa atgtactgcc ttccatctct gtttctaaaa
    50221 taatattgtt gtacttatct agtagttctt tcacattatt taatccgact ccgcgatttc
    50281 ttcccttagt ggagaatcct aaggcaaata gatctcctga aggagtcatc gtcattttac
    50341 atgaattctg aatcaccata actgtttcag tttccatctt aataactgct acttccatct
    50401 gctttttata gctatcagcc gatccttcga cagcattatt caataaaacg ctcatgatac
    50461 gaaccaaatc caatagttca attggaagct tggtaatcgt atcttttact tccagtgtaa
    50521 actctacacc attatttcga gcatagacaa ttgactgagc aaccaaactt cgtaaagctg
    50581 agtcttctat gttgttcaaa tcaaagtaag tgtacttatc tgaacgcaat ttatgatttg
    50641 ctttgactaa aacttcattg taaattctgt caatttcctg taaattacca ctgtcaattg
    50701 ccatctgcat gctgacaagc attccagcat aatcatgtcg aaaaccacgg atttcattat
    50761 acagaccaac aatttcatct gtgtaattct gtaaatgttt ctgttcaaat ttcttctgct
    50821 taaaagcaat ctctttctcc atttgaactt tatgagaatt cattgcaaag aaggtcaaaa
    50881 ggagagagat aaagacaata gatgacaaaa tacttccaaa actattcaaa tgtttaatcg
    50941 tacttaccat atctgaaacg aaagatacaa tatgtagcaa tagtaaagca aaaaatactt
    51001 ttttcaagaa aggataaagg tagtccttgt caaaataggc tagttccaaa tggaaataat
    51061 aaatgatttt taatgtaaca aaataggtta acaccgtcac aacgaaaaag aatgggaaat
    51121 gatattgtaa aacaaaatta tctcctgtta tagaggagaa agttacggac agaaagttat
    51181 gagtgctctc atataaaaga gatagtagta aacttaggaa tagtcctcta tccctctcat
    51241 actgtttcat ccatcgaaaa taggaatata agcccaaagg aaataaaaat ctttcaatcc
    51301 ctattttatc taaatataga agataaaagg aaaattcaag tactatttca gttagtaatg
    51361 tataagcacc aaaaatgtat aattcttttc tatttattcg acctttacaa attaaacggt
    51421 aactgtgact aataattaaa aaatgaacaa taactgtccc aaatccaagt aaatccatta
    51481 ctctttctcc ttatttcatt acttttttcg taggaaaaga aaatcaagga tgattcttga
    51541 aatcctcatc tccccacctt taatattttg taagtctttt tccttcaaag ctacaaactg
    51601 ttccaattta actgtgtttt tcataataaa atctcctaaa atgttttttc ttgtaagcta
    51661 acttacaaaa accattatac aaaatggaat ttcactttag ataaaattct ctcaactgtc
    51721 atttttttct cccaaagtgt acttttttaa gaaaaaagcc gggaaaattc ccagctttgc
    51781 tattatattg atcccagcag gattcgaacc tgcgaccgtt cgcttagaag gcgaatgctc
    51841 tatccagctg agctatgaga cctaacacaa ttattctacc aaaaattcaa ttaaaagtca
    51901 attttctatt tatggtaggg gaatccctgc tgaatcgtaa aagcgcgata gatttgttca
    51961 acaagaacta gtctcattaa ctgatgaggc aaggttaggc gaccaaaact gacagaaaga
    52021 ttggctctat tttttacaga tgatgacaat cctaaacttc ccccaataat aaaagtaaga
    52081 gtagaaaatc cttttataga agtttcttct aactgcttac taaattcttc tgagaagaaa
    52141 gttttccctt caatggctaa cacaataacg aaatcacggt cagcaatttt tgataaaatt
    52201 ctctgacctt ctatttctaa aatcttttga ttttctgatt cactggcctt atctggtgtt
    52261 ttttcatctg ataactcaat catttcaaac ttagcaaatc tagaaattcg ttttgaatac
    52321 tctgcgatac catcttttaa atacttttct ttcagtttcc caactgttac aactttaatt
    52381 ttcatgactc tattctaaca tattctctat tttttcacat cttattcaca aaataaaaaa
    52441 tagatttcaa ttaagaaaat cacagtttca aaagagttat ccacagtttg tgtaaaactt
    52501 ttgtgtttaa gttataatta agctagtcag tttatacttt cagtaattca aacatatgga
    52561 ggcaaatatg aaacatctaa aaacatttta caaaaaatgg tttcaattat tagtcgttat
    52621 cgtcattagc ttttttagtg gagccttggg tagtttttca ataactcaac taactcaaaa
    52681 aagtagtgta aacaactcta acaataatag tactattaca caaactgcct ataagaacga
    52741 aaattcaaca acacaggctg ttaacaaagt aaaagatgct gttgtttctg ttattactta
    52801 ttcggcaaac agacaaaata gcgtatttgg caatgatgat actgacacag attctcagcg
    52861 aatctctagt gaaggatctg gagttattta taaaaagaat gataaagaag cttacatcgt
    52921 caccaacaat cacgttataa atggcgccag caaagtagat attcgattgt cagatgggac
    52981 taaagtacct ggagaaattg tcggagctga cactttctct gatattgctg tcgtaaaaat
    53041 ctcttcagaa aaagtgacaa cagtagctga gtttggtgat tctagtaagt taactgtagg
    53101 agaaactgct attgccatcg gtagcccgtt aggttctgaa tatgcaaata ctgtcactca
    53161 aggtatcgta tccagtctca atagaaatgt atccttaaaa tcggaagatg gacaagctat
    53221 ttctacaaaa gccatccaaa ctgatactgc tattaaccca ggtaactctg gcggcccact
    53281 gatcaatatt caagggcagg ttatcggaat tacctcaagt aaaattgcta caaatggagg
    53341 aacatctgta gaaggtcttg gtttcgcaat tcctgcaaat gatgctatca atattattga
    53401 acagttagaa aaaaacggaa aagtgacgcg tccagctttg ggaatccaga tggttaattt
    53461 atctaatgtg agtacaagcg acatcagaag actcaatatt ccaagtaatg ttacatctgg
    53521 tgtagttgtt cgttcggtac aaagtaatat gcctgccaat ggtcaccttg aaaaatacga
    53581 tgtaattaca aaagtagatg acaaagagat tgcttcatca acagacttac aaagtgctct
    53641 ttacaaccat tctatcggag acaccattaa gataacctac tatcgtaacg ggaaagaaga
    53701 aactacctct atcaaactta acaagagttc aggtgattta gaatcttaat tgacatctat
    53761 gtaaagaaag ctttacataa gagaaaagat gtgttagtgt agaatcatgg aaaaatttga
    53821 aatgatttct atcacagata tacaaaaaaa tccctatcaa ccccgaaaag aatttgatag
    53881 agaaaagcta gatgaactag cacagtctat caaagaaaat ggggtcattc aaccgattat
    53941 tgttcgtcaa tctcctgtta ttggttatga aatccttgca ggagagagac gctatcgggc
    54001 ttcactttta gctggtctac ggtctatccc agctgttgtt aaacagattt cagaccaaga
    54061 gatgatggtc cagtccatta ttgaaaattt acagagagaa aatttaaacc caatagaaga
    54121 agcacgcgcc tatgaatctc tcgtagagaa aggattcacc catgctgaaa ttgcagataa
    54181 gatgggcaag tctcgtccat atatcagcaa ctccattcgt ttactttcct tgccagaaca
    54241 gattctctca gaagtagaaa atggcaaact atcacaagcc catgcgcgtt ccctagttgg
    54301 gttaaataag gaacaacaag actatttctt tcaacggatt atagaagaag atatttctgt
    54361 aaggaagtta gaaactcttc tgacagagaa aaaacaaaag aaacagcaaa aaactaatca
    54421 tttcatacaa aatgaagaaa aacagttaag aaaactactc ggattagatg tagaaattaa
    54481 actatctaaa aaagacagtg gaaaaatcat tatttctttt tcaaatcaag aagaatatag
    54541 tagaattatc aacagcctga aataaggctg ttcttttatt tttttatctc acaaggttat
    54601 ccactatgtt tttcgataaa aagcttaata aatcaataat ttcttctttt atccccaacc
    54661 tgtggataaa gtttggtaac attgtggatt atttttcaca gcttgtggaa aattcttgct
    54721 atctatggta aaatatctct agtattaaac ttttaaatag taaaggagga gaaaggattg
    54781 aaagaaaaac aattttggaa tcgtatatta gaatttgcac aagaaagact gactcgatcc
    54841 atgtatgatt tctatgctat tcaagctgaa ctcatcaagg tagaggaaaa tgttgccact
    54901 atatttctac ctcgctctga aatggaaatg gtctgggaaa aacaactaaa agatattatt
    54961 gtagtagctg gttttgaaat ttatgacgct gaaataactc cccactatat tttcaccaaa
    55021 cctcaagata cgactagctc acaagttgaa gaagctacaa atttaactct ttatgactat
    55081 agtccaaagt tagtatctat tccttattca gatacgggat taaaagaaaa gtataccttt
    55141 gataacttta ttcaagggga tggaaatgtt tgggctgtat cagccgcttt agctgtctct
    55201 gaagatttgg ctctgaccta taaccctctt tttatctatg gaggaccagg ccttggtaag
    55261 actcacttat taaacgctat tggaaatgaa attctaaaaa atattcctaa tgcgcgtgtt
    55321 aaatatatcc ctgccgaaag ctttattaat gactttcttg atcacctaag acttggggaa
    55381 atggaaaagt ttaaaaagac ctatcgtagt cttgatcttt tgttaatcga tgatatacag
    55441 tcactcagcg gaaaaaaagt cgcaactcag gaagaatttt tcaatacctt taacgccctt
    55501 catgacaagc aaaaacagat tgtcctaacg agtgatcgta gtccaaaaca tctagaaggg
    55561 ctcgaggaga ggcttgtcac gcgttttagt tggggattga cacaaactat cacaccccct
    55621 gactttgaaa cacgtattgc cattttacaa agtaagacgg aacatttagg ctacaatttc
    55681 caaagtgata ctctagaata cctagctggg caatttgatt caaatgttcg agatcttgag
    55741 ggagccatca acgacatcac tttaattgcc agagtaaaaa aaatcaagga tatcactatt
    55801 gatattgctg cagaagccat tagagcccgc aaacaagatg ttagccaaat gctcgtcatc
    55861 ccaattgata aaatccaaac tgaagttggt aacttttatg gtgttagtat caaagaaatg
    55921 aagggaagta gacgccttca aaatattgtt ttggcccgtc aagtagccat gtatttatct
    55981 agagaactaa cagataatag tcttccaaaa attgggaagg aatttggggg aaaagatcat
    56041 accacagtca ttcatgccca tgccaaaata aaatctttga ttgatcaaga cgataattta
    56101 cgtttagaaa ttgaatcaat caaaaagaaa atcaaataat ttgtggataa cttttagttt
    56161 tttatctttt ttatccacat tttttaaaca agctaaaaaa cttgatatga cttgtttaaa
    56221 ggctgttttc cacagatttc acagactcta ttattactat tatctttcta atactaaaaa
    56281 taaataaagg agaatccatg attcattttt caattaataa aaatttattt ctacaagcat
    56341 taaatactac taagagagct attagttcta aaaatgccat tcctatttta tcaacagtaa
    56401 aaattgacgt gaccaatgaa ggtattactt taattggttc aaatggtcaa atttcaattg
    56461 aaaattttat ttctcaaaaa aatgaagatg ctggtttgtt aattacttct ttaggttcga
    56521 tccttcttga agcttctttc tttatcaatg tagtatctag tttacctgat gtgactcttg
    56581 attttaaaga aattgaacaa aatcaaattg ttttaaccag tggcaaatca gaaattaccc
    56641 taaaaggaaa agatagcgaa caatatccac gaatccaaga aatttcagca agcactcctt
    56701 taatacttga aacaaaatta ctcaagaaaa ttattaatga aacagccttt gctgcaagta
    56761 cacaagagag tcgtccgatt ttaacaggtg tccacttcgt attgagtcaa cacaaagagt
    56821 taaaaacagt tgcaacagac tctcatcgcc taagccagaa aaaattgact cttgaaaaaa
    56881 atagtgatga ttttgatgtc gtaattccta gccgttctct acgcgaattt tcagcggtat
    56941 ttacagatga tatcgaaact gtagagattt tctttgccaa taaccaaatc ctctttagaa
    57001 gcgaaaatat tagcttctat actcgtctcc tagaaggaaa ctatcctgat acagatcgct
    57061 tgattccaac agactttaac actactatta cttttaatgt ggtaaactta cgccagtcaa
    57121 tggagcgtgc ccgtctttta tcaagtgcga ctcaaaatgg tactgtgaaa cttgaaatta
    57181 aggatggggt tgttagcgcc catgttcact ctccagaagt tggtaaagta aacgaagaaa
    57241 tcgatactga tcaggttact ggtgaagatt tgaccattag tttcaaccca acttacttga
    57301 ttgattctct taaagcttta aatagcgaaa aggtgaccat tagctttatc tcagctgttc
    57361 gtccatttac tcttgtgcca gcagatactg acgaagactt catgcagctc attacaccag
    57421 ttcgtacaaa ttaagtgaaa gaggttgagc ctggctcgcc tcttttatga tataatcgaa
    57481 aaagaaaagg agagtagtat gtatcaagtt ggaaattttg ttgagatgaa aaaatcacac
    57541 gcttgtacaa tcaagtcgac tggtaaaaag gctaatcgtt gggaagttac acgtgtagga
    57601 gcagatatca aaataaaatg cagtaattgt gagcatgttg tcatgatggg gcgatatgat
    57661 tttgagcgaa aaatgaataa aattattgac tgagaaccct tagttagagg gttagcactt
    57721 tatccctttt tgtgttataa tattagggat tgaaatgaaa acggagaatg agaaatatgg
    57781 ctttgacagc aggtatcgtt ggtttgccaa acgttggtaa atcaacatta tttaatgcaa
    57841 ttacaaaagc aggagcagag gcagcaaact acccatttgc gactattgat ccaaatgttg
    57901 gaatggtgga agttccagat gaacgcctac aaaaactaac tgaaatgata actcctaaaa
    57961 agacagttcc cacaacattt gaatttacag atattgcagg gattgtaaaa ggagcttcaa
    58021 aaggagaagg gctagggaat aaattcttgg ccaatattcg tgaagtagat gcgattgttc
    58081 acgtagttcg tgcttttgat gatgaaaatg tgatgcgcga gcaaggacgt gaagacgcct
    58141 ttgtagatcc acttgcagat attgatacaa ttaatctgga attgattctt gctgacttag
    58201 aatcagtgaa caaacgatat gcgcgtgtag aaaagatggc acgtacgcaa aaagataaag
    58261 aatcagtagc agaattcaat gttcttcaaa agattaaacc agtcctagaa gacgggaaat
    58321 cagctcgtac cattgaattt acagatgagg aacaaaaggt tgtcaaaggt cttttccttt
    58381 tgacgactaa accagttctt tatgtagcta atgtggacga ggatgtggtt tcagaacctg
    58441 actctatcga ctatgtcaaa caaattcgtg aatttgcagc gacagaaaat gctgaagtag
    58501 tcgttatttc tgcgcgtgct gaggaagaaa tttctgaatt ggatgatgaa gataaaaaag
    58561 agtttcttga agccattggt ttgacagaat caggtgtaga taagttgacg cgtgcagctt
    58621 accacttgct tggattggga acttacttca cagctggtga aaaagaagtt cgcgcttgga
    58681 ctttcaaacg tggtatgaag gctcctcaag cagctggtat tatccactca gactttgaaa
    58741 aaggctttat tcgtgcagta accatgtcat atgaagatct agtgaaatac ggatctgaaa
    58801 aggccgtaaa agaagctgga cgcttgcgtg aagaaggaaa agaatatatc gttcaagatg
    58861 gcgatatcat ggaattccgc tttaatgtct aaaaattaat aaatggtgtc aattaggttg
    58921 gaaaaaaaat tccaaccctt ttggcttttg aaaggaaaaa taaatgacca aattacttgt
    58981 aggcttggga aatccagggg ataaatattt tgaaacaaaa cacaatgttg gttttatgtt
    59041 gattgatcaa ctagcgaaga aacagaatgt cacttttaca cacgataaga tatttcaagc
    59101 tgacctagca tcctttttcc taaatggaga aaaaatttat ctggttaaac caacgacctt
    59161 tatgaatgaa agtggaaaag cagttcatgc tttattaact tactatggtt tggatattga
    59221 cgatttactt atcatttacg atgatcttga catggaagtt gggaaaattc gtttaagagc
    59281 aaaaggctca gcaggtggtc ataatggtat caagtctatt attcaacata taggaactca
    59341 ggtctttaac cgtgttaaga ttggaattgg aagacctaaa aatggtatgt cagttgttca
    59401 tcatgttttg agtaagtttg acagggatga ttatatcggt attttacagt ctattgacaa
    59461 agttgacgat tctgtaaact actatttaca agagaaaaat tttgagaaaa caatgcagag
    59521 gtataacgga taaatggtga ccttattaga tttattctca gaaaatgatc agattaaaaa
    59581 atggcatcaa aatttaacag ataagaaaag acaactaata cttggtttat caacatctac
    59641 taaggctctt gcaattgcaa gcagtttaga aaaagaagat aggattgtgt tattgacgtc
    59701 aacttatgga gaagcagaag gacttgttag tgatcttata tctatcttgg gtgaggaact
    59761 cgtctatcca tttttggtag atgatgctcc tatggtggag tttttgatgt cttcacagga
    59821 aaaaattatt tcacgggttg aagccttgcg ttttttgact gattcatcta agaaagggat
    59881 tttagtttgt aatatcgtag caagtcgatt gattttaccg tctcccaatg cattcaaaga
    59941 tagtattgta aaaatctcag ttggtgaaga atatgatcaa cacgcgttta tccatcagtt
    60001 aaaggaaaat ggctatcgaa aagttactca agtacaaact cagggcgaat ttagtcttcg
    60061 aggagatatt ttagatattt ttgaaatatc ccagttagaa ccttgtcgaa ttgagttttt
    60121 tggtgatgaa attgatggta tcaggtcatt tgaagtagaa acacaattat cgaaagaaaa
    60181 taagacagaa ctcactatct ttccagctag tgatatgctt ttgagagaaa aggattatca
    60241 acgaggacag tcagctttag aaaaacaaat ttcaaaaact ttatcaccta ttttgaaatc
    60301 atacctagaa gaaattcttt caagttttca ccaaaaacaa agtcatgcag actctcggaa
    60361 gtttttatct ttgtgctatg ataagacatg gactgtcttt gattatattg aaaaagatac
    60421 cccaatattc tttgatgatt atcaaaaatt gatgaatcag tatgaagtct ttgaaagaga
    60481 cttagcgcag tactttacag aagaattaca gaatagtaaa gcattttctg atatgcagta
    60541 tttttctgat attgaacaaa tctataaaaa acaaagtcca gtgacctttt tctctaatct
    60601 tcaaaagggt ttaggaaatc tcaaatttga caaaatttat caattcaatc aatatcctat
    60661 gcaggaattt ttcaatcagt tttcttttct aaaagaagaa attgaacgat ataaaaaaat
    60721 ggattacacc attattctgc agtctagcaa ttcaatggga agtaaaacat tggaggatat
    60781 gttagaggaa tatcagatta aattggattc tagagataag acaaatatct gtaaagaatc
    60841 tgtaaactta atagagggta atctcagaca tggttttcat tttgtagatg aaaagatttt
    60901 attgataact gaacatgaga tttttcaaaa gaaattaaag cgtcgttttc gaagacaaca
    60961 tgtttcaaat gcagagagat taaaagatta caatgaactt gaaaaagggg actatgttgt
    61021 ccatcatatc catgggattg gtcaatatct aggaattgaa accattgaaa tcaagggaat
    61081 tcatcgcgat tatgtcagtg tccaatacca aaatggtgat caaatttcta tccccgtgga
    61141 acagattcat ctactgtcca aatatatttc aagtgatggt aaagctccaa aactcaataa
    61201 attaaatgac ggtcatttta aaaaggccaa gcaaaaggtt aagaaccagg tagaggatat
    61261 agctgatgat ttaatcaaac tctactctga acgtagtcag ttgaagggtt ttgctttctc
    61321 agctgatgat gatgatcaag atgcctttga tgatgctttc ccttatgttg aaacggatga
    61381 tcaacttcgt agtattgagg aaatcaagag ggatatgcag gcttctcagc caatggatcg
    61441 acttttagtt ggggatgttg gttttggaaa gactgaagtt gctatgcgtg cagcctttaa
    61501 agcagtcaat gatcacaaac aggttgtcat tctagttccg acgacggttt tagcgcaaca
    61561 gcactatacg aattttaagg aacgattcca aaattttgca gttaatattg atgtgttgag
    61621 tcgctttaga agtaaaaaag agcagactgc aacacttgaa aaattgaaaa acggtcaagt
    61681 cgatattttg attggaacac atcgtgtttt gtcaaaagat gttgtgtttg ctgatttggg
    61741 cttgatgatt attgatgagg aacagcgatt tggtgtcaag cataaggaaa ctttgaaaga
    61801 actgaagaaa caagtggatg tcctaacctt gaccgctacg ccaatccctc gtaccctcca
    61861 tatgtctatg ctgggaatca gagatttatc tgttattgaa actccgccga ctaatcgcta
    61921 tcctgttcag acctatgttt tggaaaagaa tgatagtgtc attcgtgatg ctgtcttgcg
    61981 tgaaatggag cgtggaggtc aagtttatta tctttacaac aaagttgaca caattgttca
    62041 gaaggtttca gaattacagg agttgattcc ggaggcttcg attggatatg ttcatggtcg
    62101 aatgagtgaa gtccagttgg aaaatactct attagacttt attgagggac aatacgatat
    62161 cttggtgacg actactatta ttgagacagg ggtggacatt ccaaatgcta atactttatt
    62221 tattgaaaat gcggaccata tgggcttgtc aaccttatat cagttaagag gaagagtcgg
    62281 tcgtagtaat cgtattgctt atgcttatct catgtatcgt ccagaaaaat caatcagtga
    62341 agtctctgaa aagagattag aagcgattaa aggatttaca gaattgggct ctggctttaa
    62401 gattgcaatg cgagatcttt cgattcgtgg agcaggaaat cttttaggaa aatcccagtc
    62461 tggtttcatt gattctgttg gttttgaatt gtattcgcag ttattagagg aagctattgc
    62521 taaacgaaac ggtaatgcta acgctaacac aagaaccaaa gggaatgctg agttgatttt
    62581 gcaaattgat gcctatcttc ctgatactta tatttctgat caacgacata agattgaaat
    62641 ttacaagaaa attcgtcaaa ttgacaaccg tgtcaattat gaagagttac aagaggagtt
    62701 gatagaccgt tttggagaat acccagatgt agtagcctat cttttagaga ttggtttggt
    62761 taaatcatac ttggacaagg tctttgttca acgtgtggaa agaaaagata ataaaattac
    62821 aattcaattt gaaaaagtca ctcaacggct gtttttagct caagattatt ttaaagcttt
    62881 atccgtaacg aacttaaaag caggcatcgc tgagaataag ggattaatgg agcttgtatt
    62941 tgatgtccaa aataagaaag attatgaaat tttagaaggt ctgctgattt ttggagaaag
    63001 tttattagag ataaaagagt ttaaggaaga aaattccatt tgatattttt cttctataaa
    63061 atagataaaa atggtacaat aataaattga ggtaataagg atgagattag ataaatattt
    63121 aaaagtatcg cgaattatca agcgtcgtac agtcgcaaag gaagtagcag ataaaggtag
    63181 aatcaaggtt aatggaatct tggccaaaag ttcaacggac ttgaaagtta atgaccaagt
    63241 tgaaattcgc tttggcaata agttgctgct tgtaaaagta ctagagatga aagatagtac
    63301 aaaaaaagaa gatgcagcag gaatgtatga aattatcagt gaaacacggg tagaagaaaa
    63361 tgtctaaaaa tattgtacaa ttgaataatt cttttattca aaatgaatac caacgtcgtc
    63421 gctacctgat gaaagaacga caaaaacgga atcgttttat gggaggggta ttgattttga
    63481 ttatgctatt atttatcttg ccaactttta atttagcgca gagttatcag caattactcc
    63541 aaagacgtca gcaattagca gacttgcaaa ctcagtatca aactttgagt gatgaaaagg
    63601 ataaggagac agcatttgct accaagttga aagatgaaga ttatgctgct aaatatacac
    63661 gagcgaagta ctattattct aagtcgaggg aaaaagttta tacgattcct gacttgcttc
    63721 aaaggtgata aaatggaaaa tttattagac gtaatagagc aatttttgag tttgtcagat
    63781 gaaaagctgg aagaattggc tgataaaaat caattattgc gtttacaaga agaaaaggaa
    63841 aggaagaatg cgtaaattct taattatttt gttgctacca agttttttga ccatttcaaa
    63901 agtcgttagc acagaaaaag aagtcgtcta tacttcgaaa gaaatttatt acctttcaca
    63961 atctgacttt agtatttatt ttagagaaaa attaagttct cccatggttt atggagaggt
    64021 tcctgtttat gcgaatgaag atttagtagt ggaatctggg aaattgactc ccaaaacaag
    64081 ttttcaaata accgagtggc gcttaaataa acaaggaatt ccagtattta agctatcaaa
    64141 tcatcaattt atagctgcgg acaaacgatt tttatatgat caatcagagg taactccaac
    64201 aataaaaaaa gcatggttag aatctgactt taaactgtac aatagtcctt atgatttaaa
    64261 agaagtgaaa tcatccttat cagcttattc gcaagtatca atcgacaaga ccatgtttgt
    64321 agaaggaaga gaatttctac atattgatca ggctggatgg gtagctaaag aatcaacttc
    64381 tgaagaagat aatcggatga gtaaagttca agaaatgtta tctgaaaaat atcagaaaga
    64441 ttctttctct atttatgtta agcaactgac tactggaaaa gaagctggta tcaatcaaga
    64501 tgaaaagatg tatgcagcca gcgttttgaa actctcctat ctctattata cgcaagaaaa
    64561 aataaatgag ggtctttatc agttagatac gactgtaaaa tacgtatctg cagtcaatga
    64621 ttttccaggt tcttataaac cagagggaag tggtagtctt cctaaaaaag aagataataa
    64681 agaatattct ttaaaggatt taattacgaa agtatcaaaa gaatctgata atgtagctca
    64741 taatctattg ggatattaca tttcaaacca atctgatgcc acattcaaat ccaagatgtc
    64801 tgccattatg ggagatgatt gggatccaaa agaaaaattg atttcttcta agatggccgg
    64861 gaagtttatg gaagctattt ataatcaaaa tggatttgtg ctagagtctt tgactaaaac
    64921 agattttgat agtcagcgaa ttgccaaagg tgtttctgtt aaagtagctc ataaaattgg
    64981 agatgcggat gaatttaagc atgatacggg tgttgtctat gcagattctc catttattct
    65041 ttctattttc actaagaatt ctgattatga tacgatttct aagatagcca aggatgttta
    65101 tgaggttcta aaatgaggga accagatttt ttaaatcatt ttctcaagaa gggatatttc
    65161 aaaaagcatg ctaaggcggt tctagctctt tctggtggat tagattccat gtttctattt
    65221 aaggtattgt ctacttatca aaaagagtta gagattgaat tgattctagc tcatgtgaat
    65281 cataagcaga gaattgaatc agattgggaa gaaaaggaat taaggaagtt ggctgctgaa
    65341 gcagagcttc ctatttatat cagcaatttt tcaggagaat tttcagaagc gcgtgcacga
    65401 aattttcgtt atgatttttt tcaagaggtc atgaaaaaga caggtgcgac agctttagtc
    65461 actgcccacc atgctgatga tcaggtggaa acgattttga tgcgcttgat tcgaggaact
    65521 cgcttgcgct atctatcagg aattaaggag aagcaagtag tcggagagat agaaatcatt
    65581 cgtcccttct tgcattttca gaaaaaagac tttccatcaa tttttcactt tgaagataca
    65641 tcaaatcagg agaatcatta ttttcgaaat cgtattcgaa attcttactt accagaattg
    65701 gaaaaagaaa atcctcgatt tagggatgca atcttaggca tcggcaatga aattttagat
    65761 tatgatttgg caatagctga attatctaac aatattaatg tggaaaattt acagcagtta
    65821 ttttcttact ctgagtctac acaaagagtt ttacttcaaa cttatctgaa tcgttttcca
    65881 gatttgaatc ttacaaaagc tcagtttgct gaagttcagc agattttaaa atctaaaagc
    65941 cagtatcgtc atccgattaa aaatggctat gaattgataa aagagtacca acagtttcag
    66001 atttgtaaaa tcagtccgca ggctgatgaa gaggaagatg aacttgtgtt acactatcaa
    66061 aatcaggtag cttatcaagg atatttattt tcctttggac ttccattaga aggtgaatta
    66121 attcaacaaa tacctgtttc acgtgaaaca tccatacaca ttcgtcatcg aaaaacagga
    66181 gatgttttga ttcaaaatgg gcatagaaaa aaactcagac gtttatttat tgatttgaaa
    66241 atccctatgg aaaagagaaa ctctgctctt attattgagc aatttggtga aattgtctca
    66301 attttgggaa ttgcgaccaa taatttgagt aaaaaaacga aaaatgatat aatgaacact
    66361 gtactttata tagaaaaaat agataggtaa aaaatgttag aaaacgatat taaaaaagtc
    66421 ctcgtttcac acgatgaaat tacagaagca gctaaaaaac taggtgctca attaactaaa
    66481 gactatgcag gaaaaaatcc aatcttagtt gggattttaa aaggatctat tccttttatg
    66541 gctgaattgg tcaaacatat tgatacacat attgaaatgg acttcatgat ggtttctagc
    66601 taccatggtg gaacagcaag tagtggtgtt atcaatatta aacaagatgt gactcaagat
    66661 atcaaaggaa gacatgttct atttgtagaa gatatcattg atacaggtca aactttgaag
    66721 aatttgcgag atatgtttaa agaaagagaa gcagcttctg ttaaaattgc aaccttgttg
    66781 gataaaccag aaggacgtgt tgtagaaatt gaggcagact atacctgctt tactatccca
    66841 aatgagtttg tagtaggtta tggtttagac tacaaagaaa attatcgtaa tcttccttat
    66901 attggagtat tgaaagagga agtgtattca aattagaaag aataatcttt aatgaaaaaa
    66961 caaaataatg gtttaattaa aaatcctttt ctatggttat tatttatctt tttccttgtg
    67021 acaggattcc agtatttcta ttctgggaat aactcaggag gaagtcagca aatcaactat
    67081 actgagttgg tacaagaaat taccgatggt aatgtaaaag aattaactta ccaaccaaat
    67141 ggtagtgtta tcgaagtttc tggtgtctat aaaaatccta aaacaagtaa agaaggaaca
    67201 ggtattcagt ttttcacgcc atctgttact aaggtagaga aatttaccag cactattctt
    67261 cctgcagata ctaccgtatc agaattgcaa aaacttgcta ctgaccataa agcagaagta
    67321 actgttaagc atgaaagttc aagtggtata tggattaatc tactcgtatc cattgtgcca
    67381 tttggaattc tattcttctt cctattctct atgatgggaa atatgggagg aggcaatggc
    67441 cgtaatccaa tgagttttgg acgtagtaag gctaaagcag caaataaaga agatattaaa
    67501 gtaagatttt cagatgttgc tggagctgag gaagaaaaac aagaactagt tgaagttgtt
    67561 gagttcttaa aagatccaaa acgattcaca aaacttggag cccgtattcc agcaggtgtt
    67621 cttttggagg gacctccggg gacaggtaag actttgcttg ctaaggcagt cgctggagaa
    67681 gcaggtgttc cattctttag tatctcaggt tctgactttg tagaaatgtt tgtcggagtt
    67741 ggagctagtc gtgttcgctc tctttttgag gatgccaaaa aagcagcacc agctatcatc
    67801 tttatcgatg aaattgatgc tgttggacgt caacgtggag tcggtctcgg cggaggtaat
    67861 gacgaacgtg aacaaacctt gaaccaactt ttgattgaga tggatggttt tgagggaaat
    67921 gaagggatta tcgtcatcgc tgcgacaaac cgttcagatg tacttgatcc tgcccttttg
    67981 cgtccaggac gttttgatag aaaagtattg gttggccgtc ctgatgttaa aggtcgtgaa
    68041 gcaatcttga aagttcacgc taagaacaag cctttagcag aagatgttga tttgaaatta
    68101 gtggctcaac aaactccagg ctttgttggt gctgatttag agaatgtctt gaatgaagca
    68161 gctttagttg ctgctcgtcg caataaatcg ataattgatg cttcagatat tgatgaagca
    68221 gaagatagag ttattgctgg accttctaag aaagataaga cagtttcaca aaaagaacga
    68281 gaattggttg ctcaccatga ggcaggacat accattgttg gtctagtctt gtcgaatgct
    68341 cgcgttgtcc ataaggttac aattgtacca cgcggccgtg caggcggata catgattgca
    68401 cttcctaaag aggatcaaat gcttctatct aaagaagata tgaaagagca attggctggc
    68461 ttaatgggtg gacgtgtagc tgaagaaatt atctttaatg tccaaactac aggagcttca
    68521 aacgactttg aacaagcgac acaaatggca cgtgcaatgg ttacagagta cggtatgagt
    68581 gaaaaacttg gcccagtaca atatgaagga aaccatgcta tgcttggtgc acagagtcct
    68641 caaaaatcaa tttcagaaca aacagcttat gaaattgatg aagaggttcg ttcattatta
    68701 aatgaggcac gaaataaagc tgctgaaatt attcagtcaa atcgtgaaac tcacaagtta
    68761 attgcagaag cattattgaa atacgaaaca ttggatagta cacaaattaa agctctttac
    68821 gaaacaggaa agatgcctga agcagtagaa gaggaatctc atgcactatc ctatgatgaa
    68881 gtaaagtcaa aaatgaatga cgaaaaataa ccctgagaga ggctggagtc tctctttttt
    68941 gtgcagttta ggagctaaag ggaacagaat ggagaaaatg gaacaaatgt gttttctaat
    69001 ctgttagact gtatctagaa aggggaaaat tatgattaaa gaattgtatg aagaagtcca
    69061 agggactgtg tataagtgta gaaatgaata ttaccttcat ttatgggaat tatcggattg
    69121 ggaccaagaa ggcatgctct gcttacatga attgattagt agagaagaag gactggtaga
    69181 cgatattcca cgtttaagga aatatttcaa aaccaagttt cgaaatcgaa ttttagacta
    69241 tatccgtaag caggaaagtc agaagcgtag atacgataaa gaaccctatg aagaagtggg
    69301 tgagatcagt catcgtataa gtgagggggg tctctggcta gatgattatt atctctttca
    69361 tgaaacacta agagattata gaaacaaaca aagtaaagag aaacaagaag aactagaacg
    69421 cgtcttaagc aatgaacgat ttcgagggcg tcaaagagta ttaagagact tacgcattgt
    69481 gtttaaggag tttactatcc gtacccacta gtaagtcatg caaaaaaaaa tgaaaaaaat
    69541 tagaaaaagt agttgacaaa gtttgaaaag actgtataat agtaagagtt gaaaataaca
    69601 actcaggtcc gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc
    69661 gaatcccgta cggactatgg tatgttgcgt caggagcact tgatgaaaaa aagtttaaaa
    69721 aagcttaaaa atcttcaaaa aagtgttgac aagtgaaagt ggctgtgata tactaatata
    69781 gttgtcgctt gagagaagca agtgacaaag acctttgaaa actgaacaag acgaaccaat
    69841 gtgcagggcg ctacaacata agttgtagta ctgaacaatg aaaaaacaat aaatctgtca
    69901 gtgacagaaa tgagtaagaa ctcaaacttt taatgagagt ttgatcctgg ctcaggacga
    69961 acgctggcgg cgtgcctaat acatgcaagt agaacgctga aggaggagct tgcttctctg
    70021 gatgagttgc gaacgggtga gtaacgcgta ggtaacctgc ctggtagcgg gggataacta
    70081 ttggaaacga tagctaatac cgcataagag tagatgttgc atgacatttg cttaaaaggt
    70141 gcacttgcat cactaccaga tggacctgcg ttgtattagc tagttggtgg ggtaagggct
    70201 caccaaggcg acgatacata gccgacctga gagggtgatc ggccacactg ggactgagac
    70261 acggcccaga ctcctacggg aggcagcagt agggaatctt cggcaatgga cggaagtctg
    70321 accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaagctctg ttgtaagaga
    70381 agaacgagtg tgagagtgga aagttcacac tgtgacggta tcttaccaga aagggacggc
    70441 taactacgtg ccagcagccg cggtaatacg taggtcccga gcgttgtccg gatttattgg
    70501 gcgtaaagcg agcgcaggcg gttagataag tctgaagtta aaggctgtgg cttaaccata
    70561 gtaggctttg gaaactgttt aacttgagtg caagagggga gagtggaatt ccatgtgtag
    70621 cggtgaaatg cgtagatata tggaggaaca ccggtggcga aagcggctct ctggcttgta
    70681 actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac
    70741 gctgtaaacg atgagtgcta ggtgttagac cctttccggg gtttagtgcc gtagctaacg
    70801 cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg
    70861 ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag
    70921 gtcttgacat ccctctgacc gctctagaga tagagttttc cttcgggaca gaggtgacag
    70981 gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc
    71041 gcaaccccta ttgttagttg ccatcattta gttgggcact ctagcgagac tgccggtaat
    71101 aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac
    71161 acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct aatctcttaa
    71221 agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg aatcgctagt
    71281 aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca
    71341 caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc cagccgccta
    71401 aggtgggata gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct
    71461 ggatcacctc ctttctaagg ataaggaact gcgcattggt cttgtttagt cttgagaggt
    71521 cttgtggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt
    71581 cgatcccgct aggctccatt ggtgagagat caccaagtaa tgcacattga aaattgaata
    71641 tctatatcaa atagtaacaa gaaaataaac cgaaaacgct gtagtattaa taagagttta
    71701 tgactgaaag gtcagaaaat aaggttaagt taataagggc gcacggtgga tgccttggca
    71761 ctaggagccg acgaaggacg tgacaaacga cgatatgcct tgggtagctg taagtaagcg
    71821 atgatccagg gatttccgaa tgggggaacc caacaggtaa tacctgttac ctacatctgt
    71881 taaggatgtg aggaggaaga cgcagtgaac tgaaacatct aagtagctgc aggaagagaa
    71941 agcaaaagcg attgccttag tagcggcgag cgaaacggca gaagggcaaa ccgaagagtt
    72001 tactcttcgg ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga atgatttggg
    72061 aagatcagcc aaagagagta atagcctcgt atttaaaata gtctttgtac ttagcagtat
    72121 cctgagtacg gcgggacacg tgaaatcccg tcggaatctg ggaggaccat ctcccaaccc
    72181 taaatactcc ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc
    72241 cgggagggga gtgaaataga acctgaaacc gtgtgcctac aacaagttcg agcccgttaa
    72301 tgggtgagag cgtgcctttt gtagaatgaa ccggcgagtt acgttatgat gcgaggttaa
    72361 gttgaagaga cggagccgta gggaaaccga gtctgaatag ggcgccttag tatcatgacg
    72421 tagacccgaa accatgtgac ctacccatga gcaggttgaa ggtgcggtaa gacgcactgg
    72481 aggaccgaac cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc ggagaaattc
    72541 caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag cgtcgacatt
    72601 agagattctt ggaggtagag cactgtttgg gtgaggggtc catcccggat taccaatctc
    72661 agataaactc cgaatgccaa tgaattatgg tcggcagtca gactgcgagt gctaagatcc
    72721 gtagtcgaaa gggaaacagc ccagaccacc agctaaggtc ccaaaataat tgttaagtgg
    72781 aaaaggatgt ggggttgcac agacaactag gatgttagct tagaagcagc tattcattca
    72841 aagagtgcgt aatagctcac tagtcgagtg accctgcgcc gaaaatgtac cggggctaaa
    72901 acaatttacc gaagctgtgg atacctttat aggtatggta ggagagcgtt ctatgtgtga
    72961 tgaaggtata ccgtgaggag tgctggaacg catagaagtg agaatgccgg tatgagtagc
    73021 gaaagacagg tgagaatcct gtccaccgta agactaaggt ttccagggga aggctcgtcc
    73081 gccctgggtt agtcgggacc taaggagaga ccgaaaggtg tatccgatgg acaacaggtt
    73141 gatattcctg tactagagta tgtagtgatg gagggacgca gtaggctaac taaagcagac
    73201 gattggaaga gtctgtctaa gcagtgaggt gtgaattgag tcaaatgctt aattctataa
    73261 cattgagctg tgatggggag cgaagtttag tagcgaagtt agtgacgtca cactgccaag
    73321 aaaagcttct agcgtttaaa catactctac ccgtaccgca aaccgacaca ggtagtcgag
    73381 gcgagtagcc tcaggtgagc gagagaactc tcgttaagga actcggcaaa atgaccccgt
    73441 aacttcggga gaaggggtgc tgacttaaag tcagccgcag tgaataggcc caagcaactg
    73501 tttatcaaaa acacagctct ctgctaaatc gtaagatgat gtataggggg tgacgcctgc
    73561 ccggtgctgg aaggttaaga ggagtgctta gcgtaagcga aggtatgaat tgaagcccca
    73621 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
    73681 ccgacccgca cgaaaggcgt aatgatttgg gcactgtctc aacgagagac tcggtgaaat
    73741 tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc atggagcttt
    73801 actgcagttt gatattgagt gtctgtacca catgtacagg ataggtagga gtctaagaga
    73861 tcgggacgcc agtttcgaag gagacgctgt tgggatacta cccttgtgtt atggccactc
    73921 taacccagat aggtgatccc tatcggagac agtgtctgac gggcagtttg actggggcgg
    73981 tcgcctccta aaaggtaacg gaggcgccca aaggttccct cagaatggtt ggaaatcatt
    74041 cgcagagtgt aaaggtataa gggagcttga ctgcgagagc tacaactcga gcagggacga
    74101 aagtcgggct tagtgatccg gtggttccgt atggaagggc catcgctcaa cggataaaag
    74161 ctaccctggg gataacaggc ttatctcccc caagagttca catcgacggg gaggtttggc
    74221 acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt tgggctgttc
    74281 gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc ggtccctatc
    74341 cgtcgcgggc gtaggaaatt tgagaggatc tgctcctagt acgagaggac cagagtggac
    74401 ttaccgctgg tgtaccagtt gtcttgccaa aggcatcgct gggtagctat gtagggaagg
    74461 gataaacgct gaaagcatct aagtgtgaaa cccacctcaa gatgagattt cccatgatta
    74521 tatatcagta agagccctga gagatgatca ggtagatagg ttagaagtgg aagtgtggcg
    74581 acacatgtag cggactaata ctaatagctc gaggacttat ccaaagtaac tgagaatatg
    74641 aaagcgaacg gttttcttaa gttgaataga tattcaattt tgagtaggta ttactcagag
    74701 ttaagtgacg atagcctagg agatacacct gtacccatgc cgaacacaga agttaagccc
    74761 tagaacgccg gaagtagttg ggggttgccc cctgtgaga
//
LOCUS       DAVKFH010000003        68080 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKFH010000003 DAVKFH010000000
VERSION     DAVKFH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.003"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..203
                     /locus_tag="VW006_000154"
     CDS             <1..203
                     /locus_tag="VW006_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9379076.1"
                     /translation="INELLIVGIKILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVAN
                     RWDRGLEAYKDKVYTRGIWIRD"
     gene            599..1462
                     /locus_tag="VW006_000155"
     CDS             599..1462
                     /locus_tag="VW006_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9379077.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            1748..1948
                     /locus_tag="VW006_000156"
     CDS             1748..1948
                     /locus_tag="VW006_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9379078.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            2015..2680
                     /locus_tag="VW006_000157"
     CDS             2015..2680
                     /locus_tag="VW006_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9379079.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            2752..3978
                     /locus_tag="VW006_000158"
     CDS             2752..3978
                     /locus_tag="VW006_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9379080.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4331..4405)
                     /locus_tag="VW006_000159"
                     /pseudo
     CDS             complement(4331..4405)
                     /locus_tag="VW006_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4476..5132
                     /locus_tag="VW006_000160"
     CDS             4476..5132
                     /locus_tag="VW006_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9379081.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            5122..5331
                     /locus_tag="VW006_000161"
     CDS             5122..5331
                     /locus_tag="VW006_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9379082.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPTIVERFLKFL
                     PVSIIFALILSRVVTGKVGNLPQIK"
     gene            5549..6379
                     /locus_tag="VW006_000162"
     CDS             5549..6379
                     /locus_tag="VW006_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9379083.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            6533..7387
                     /locus_tag="VW006_000163"
     CDS             6533..7387
                     /locus_tag="VW006_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9379084.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            7487..8860
                     /locus_tag="VW006_000164"
     CDS             7487..8860
                     /locus_tag="VW006_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9379085.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            8853..9914
                     /locus_tag="VW006_000165"
     CDS             8853..9914
                     /locus_tag="VW006_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9379086.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            9916..10608
                     /locus_tag="VW006_000166"
     CDS             9916..10608
                     /locus_tag="VW006_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379087.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(10637..11206)
                     /locus_tag="VW006_000167"
     CDS             complement(10637..11206)
                     /locus_tag="VW006_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9379088.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11341..11955
                     /locus_tag="VW006_000168"
     CDS             11341..11955
                     /locus_tag="VW006_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9379089.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            11957..13603
                     /locus_tag="VW006_000169"
     CDS             11957..13603
                     /locus_tag="VW006_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9379090.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            13615..14901
                     /locus_tag="VW006_000170"
     CDS             13615..14901
                     /locus_tag="VW006_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9379091.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            14953..15486
                     /locus_tag="VW006_000171"
     CDS             14953..15486
                     /locus_tag="VW006_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379092.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            15564..16034
                     /gene="nrdI"
                     /locus_tag="VW006_000172"
     CDS             15564..16034
                     /gene="nrdI"
                     /locus_tag="VW006_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9379093.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16327..17589)
                     /locus_tag="VW006_000173"
     CDS             complement(16327..17589)
                     /locus_tag="VW006_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9379094.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(17899..18357)
                     /locus_tag="VW006_000174"
     CDS             complement(17899..18357)
                     /locus_tag="VW006_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9379095.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(18354..18794)
                     /locus_tag="VW006_000175"
     CDS             complement(18354..18794)
                     /locus_tag="VW006_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9379096.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            19150..21099
                     /gene="mutL"
                     /locus_tag="VW006_000176"
     CDS             19150..21099
                     /gene="mutL"
                     /locus_tag="VW006_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9379097.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(21424..21891)
                     /locus_tag="VW006_000177"
     CDS             complement(21424..21891)
                     /locus_tag="VW006_000177"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9379098.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(21892..23097)
                     /locus_tag="VW006_000178"
     CDS             complement(21892..23097)
                     /locus_tag="VW006_000178"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9379099.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(23117..23752)
                     /gene="ribE"
                     /locus_tag="VW006_000179"
     CDS             complement(23117..23752)
                     /gene="ribE"
                     /locus_tag="VW006_000179"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9379100.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(23737..24837)
                     /gene="ribD"
                     /locus_tag="VW006_000180"
     CDS             complement(23737..24837)
                     /gene="ribD"
                     /locus_tag="VW006_000180"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9379101.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(24972..25086)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25243..25836
                     /gene="ruvA"
                     /locus_tag="VW006_000181"
     CDS             25243..25836
                     /gene="ruvA"
                     /locus_tag="VW006_000181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9379102.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            25846..26409
                     /locus_tag="VW006_000182"
     CDS             25846..26409
                     /locus_tag="VW006_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9379103.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            26406..27083
                     /locus_tag="VW006_000183"
     CDS             26406..27083
                     /locus_tag="VW006_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9379104.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(27220..28251)
                     /locus_tag="VW006_000184"
     CDS             complement(27220..28251)
                     /locus_tag="VW006_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9379105.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(28506..29189)
                     /locus_tag="VW006_000185"
     CDS             complement(28506..29189)
                     /locus_tag="VW006_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9379106.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILTSMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(29201..30145)
                     /locus_tag="VW006_000186"
     CDS             complement(29201..30145)
                     /locus_tag="VW006_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9379107.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            30277..33108
                     /gene="uvrA"
                     /locus_tag="VW006_000187"
     CDS             30277..33108
                     /gene="uvrA"
                     /locus_tag="VW006_000187"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9379108.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            33101..34162
                     /locus_tag="VW006_000188"
     CDS             33101..34162
                     /locus_tag="VW006_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9379109.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            34294..34692
                     /gene="spx"
                     /locus_tag="VW006_000189"
     CDS             34294..34692
                     /gene="spx"
                     /locus_tag="VW006_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9379110.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            34758..35327
                     /locus_tag="VW006_000190"
     CDS             34758..35327
                     /locus_tag="VW006_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9379111.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            35414..35680
                     /locus_tag="VW006_000191"
     CDS             35414..35680
                     /locus_tag="VW006_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9379112.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            35684..36103
                     /gene="ruvX"
                     /locus_tag="VW006_000192"
     CDS             35684..36103
                     /gene="ruvX"
                     /locus_tag="VW006_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9379113.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            36119..36424
                     /locus_tag="VW006_000193"
     CDS             36119..36424
                     /locus_tag="VW006_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9379114.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            36682..37932
                     /locus_tag="VW006_000194"
     CDS             36682..37932
                     /locus_tag="VW006_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9379115.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            37929..38471
                     /locus_tag="VW006_000195"
     CDS             37929..38471
                     /locus_tag="VW006_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9379116.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            38617..40149
                     /gene="cls"
                     /locus_tag="VW006_000196"
     CDS             38617..40149
                     /gene="cls"
                     /locus_tag="VW006_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9379117.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            40407..41945
                     /locus_tag="VW006_000197"
     CDS             40407..41945
                     /locus_tag="VW006_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9379118.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            42065..44272
                     /gene="nrdD"
                     /locus_tag="VW006_000198"
     CDS             42065..44272
                     /gene="nrdD"
                     /locus_tag="VW006_000198"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9379119.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            44284..44424
                     /locus_tag="VW006_000199"
     CDS             44284..44424
                     /locus_tag="VW006_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379120.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            44468..44974
                     /locus_tag="VW006_000200"
     CDS             44468..44974
                     /locus_tag="VW006_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379121.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            44967..45557
                     /gene="nrdG"
                     /locus_tag="VW006_000201"
     CDS             44967..45557
                     /gene="nrdG"
                     /locus_tag="VW006_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9379122.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            45565..46182
                     /locus_tag="VW006_000202"
     CDS             45565..46182
                     /locus_tag="VW006_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9379123.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRYASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            46448..46756
                     /gene="rpsJ"
                     /locus_tag="VW006_000203"
     CDS             46448..46756
                     /gene="rpsJ"
                     /locus_tag="VW006_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9379124.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            46973..47599
                     /gene="rplC"
                     /locus_tag="VW006_000204"
     CDS             46973..47599
                     /gene="rplC"
                     /locus_tag="VW006_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9379125.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            47624..48247
                     /gene="rplD"
                     /locus_tag="VW006_000205"
     CDS             47624..48247
                     /gene="rplD"
                     /locus_tag="VW006_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9379126.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            48247..48543
                     /locus_tag="VW006_000206"
     CDS             48247..48543
                     /locus_tag="VW006_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9379127.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            48561..49394
                     /gene="rplB"
                     /locus_tag="VW006_000207"
     CDS             48561..49394
                     /gene="rplB"
                     /locus_tag="VW006_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9379128.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            49498..49779
                     /gene="rpsS"
                     /locus_tag="VW006_000208"
     CDS             49498..49779
                     /gene="rpsS"
                     /locus_tag="VW006_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9379129.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            49791..50135
                     /gene="rplV"
                     /locus_tag="VW006_000209"
     CDS             49791..50135
                     /gene="rplV"
                     /locus_tag="VW006_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9379130.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            50148..50801
                     /gene="rpsC"
                     /locus_tag="VW006_000210"
     CDS             50148..50801
                     /gene="rpsC"
                     /locus_tag="VW006_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9379131.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            50805..51218
                     /gene="rplP"
                     /locus_tag="VW006_000211"
     CDS             50805..51218
                     /gene="rplP"
                     /locus_tag="VW006_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9379132.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            51228..51434
                     /locus_tag="VW006_000212"
     CDS             51228..51434
                     /locus_tag="VW006_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9379133.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            51459..51719
                     /gene="rpsQ"
                     /locus_tag="VW006_000213"
     CDS             51459..51719
                     /gene="rpsQ"
                     /locus_tag="VW006_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9379134.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            51745..52113
                     /gene="rplN"
                     /locus_tag="VW006_000214"
     CDS             51745..52113
                     /gene="rplN"
                     /locus_tag="VW006_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9379135.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            52191..52496
                     /gene="rplX"
                     /locus_tag="VW006_000215"
     CDS             52191..52496
                     /gene="rplX"
                     /locus_tag="VW006_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9379136.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            52520..53062
                     /gene="rplE"
                     /locus_tag="VW006_000216"
     CDS             52520..53062
                     /gene="rplE"
                     /locus_tag="VW006_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9379137.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            53080..53349
                     /gene="rpsN"
                     /locus_tag="VW006_000217"
     CDS             53080..53349
                     /gene="rpsN"
                     /locus_tag="VW006_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9379138.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            53634..54032
                     /gene="rpsH"
                     /locus_tag="VW006_000218"
     CDS             53634..54032
                     /gene="rpsH"
                     /locus_tag="VW006_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9379139.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            54224..54760
                     /gene="rplF"
                     /locus_tag="VW006_000219"
     CDS             54224..54760
                     /gene="rplF"
                     /locus_tag="VW006_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9379140.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            54844..55200
                     /gene="rplR"
                     /locus_tag="VW006_000220"
     CDS             54844..55200
                     /gene="rplR"
                     /locus_tag="VW006_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9379141.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            55218..55712
                     /gene="rpsE"
                     /locus_tag="VW006_000221"
     CDS             55218..55712
                     /gene="rpsE"
                     /locus_tag="VW006_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9379142.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            55684..55908
                     /gene="rpmD"
                     /locus_tag="VW006_000222"
     CDS             55684..55908
                     /gene="rpmD"
                     /locus_tag="VW006_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9379143.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            56053..56493
                     /gene="rplO"
                     /locus_tag="VW006_000223"
     CDS             56053..56493
                     /gene="rplO"
                     /locus_tag="VW006_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9379144.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            56506..57816
                     /gene="secY"
                     /locus_tag="VW006_000224"
     CDS             56506..57816
                     /gene="secY"
                     /locus_tag="VW006_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9379145.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            57967..58605
                     /locus_tag="VW006_000225"
     CDS             57967..58605
                     /locus_tag="VW006_000225"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9379146.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            58722..58940
                     /gene="infA"
                     /locus_tag="VW006_000226"
     CDS             58722..58940
                     /gene="infA"
                     /locus_tag="VW006_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9379147.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            58965..59081
                     /gene="rpmJ"
                     /locus_tag="VW006_000227"
     CDS             58965..59081
                     /gene="rpmJ"
                     /locus_tag="VW006_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9379148.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            59099..59464
                     /gene="rpsM"
                     /locus_tag="VW006_000228"
     CDS             59099..59464
                     /gene="rpsM"
                     /locus_tag="VW006_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9379149.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            59482..59865
                     /gene="rpsK"
                     /locus_tag="VW006_000229"
     CDS             59482..59865
                     /gene="rpsK"
                     /locus_tag="VW006_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9379150.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59908..60843
                     /locus_tag="VW006_000230"
     CDS             59908..60843
                     /locus_tag="VW006_000230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9379151.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            60855..61241
                     /gene="rplQ"
                     /locus_tag="VW006_000231"
     CDS             60855..61241
                     /gene="rplQ"
                     /locus_tag="VW006_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9379152.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            61506..61772
                     /locus_tag="VW006_000232"
     CDS             61506..61772
                     /locus_tag="VW006_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9379153.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            61782..63119
                     /locus_tag="VW006_000233"
     CDS             61782..63119
                     /locus_tag="VW006_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9379154.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            63363..64055
                     /locus_tag="VW006_000234"
     CDS             63363..64055
                     /locus_tag="VW006_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9379155.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(64158..65794)
                     /locus_tag="VW006_000235"
                     /pseudo
     CDS             complement(64158..65794)
                     /locus_tag="VW006_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(65784..66812)
                     /locus_tag="VW006_000236"
     CDS             complement(65784..66812)
                     /locus_tag="VW006_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379156.1"
                     /translation="MNITIPKGSLFTLLGPSGCGKTTLLRMIAGFNSIKDGEFYFDDT
                     KINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLKQKKVPKEELIQQTNKYLELMQI
                     AQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEPLSNLEAKLRLDMRQAIREIQH
                     EVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNI
                     IPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISD
                     SVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNRIRLRINTQKLNIFSADGSQNL
                     IKGVNHGM"
     gene            complement(66887..67993)
                     /locus_tag="VW006_000237"
                     /pseudo
     CDS             complement(66887..67993)
                     /locus_tag="VW006_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100970.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT        20321 a        12744 c        14912 g        20103 t
ORIGIN      
        1 taattaatga actattgatt gtcggtataa aaattttagt atatgaacca aatttaaacg
       61 ttagtataga taacgtaatt tttgaaaaga attttgaact atttacaaaa caaagtgatc
      121 taattgttgc aaatagatgg gataggggcc ttgaagctta taaagataaa gtttacacaa
      181 gggggatctg gataagagat taactattga tattaaaaag gcaataattt ataaaatttg
      241 tacaaaaaaa tgttaaattt tcacattttc atagtatact gtttaataga aagtaggttc
      301 tttgtaaccg ctccattata aaatcctttc actcttccgg tatactatta ggaaaaacat
      361 aactggagga tattttgagc tcacacctca tcgttccagt ggctgttccc caagctcgtg
      421 tactgcccaa aaaagttaga cattctattt agaacgagga ttgagttctg tattgtacaa
      481 ggctcggtcc ttttagagtc agcttaaggc tggctttttt attttttcta actatcagaa
      541 tattttgaca aatgaacaca aataatgata taatatgcaa aagctaggag gtggtaggat
      601 gattgaaaaa atggaattgg gggaatttta caaggaatta cgtttggcga gaaagctcaa
      661 gcagtcagat gtggcttgtg acgggctaac agcttctcag ttgtccaagt ttgagctagg
      721 gcagtccatg ttgtctgctg ataagctcat attagccatc caagggatca atgtgacctt
      781 tgatgagttt gggcacaagc tcaacaacta tcaagaatct ccacatatgc agattggtag
      841 aaaggttgtg gatcgctttg ctcatcaaga tatagctggc ttagagcaac tgttggagga
      901 agtcgagcaa gaacagatgg cagagactta tcgtcgtttg aatgctattg taatcaaaga
      961 tgccatccac tcactagata aaagctaccc actagcagag gaggatagcg agtttttgac
     1021 cacctatctc tatgctatcg agtcttggac ctggtttgaa ctctatctct tttgcaatac
     1081 tctgcccttc ttgagcaatc aagatctgat ctttttatca acctccttac tcgaaaaatc
     1141 caaagaattt aaagagttag tacacaatcg attgtatatg aagcaaggac tcttaaatat
     1201 catctcagag ctcatggagc gcaaactttt ttcttatatc ccaatctttg aagccgagct
     1261 ggagagtatg ctccgtccat acgatgtttt tgagaaagtg ttgtggcagt ttttaaagaa
     1321 gatgagcatt ttccttcaaa ctaagggaag caatcaaaaa gagattgaac actttatcca
     1381 atctctgcaa gtattagaaa atccacaatt aatagccctt tttgaattgc gtttgcagca
     1441 atacaaaaag cttatcgatt agttagaaat ggcaaaataa taataatctc agcaacgatt
     1501 ttcaataatg ttaaaatttg tttcttcata tttctccact cctcattagt agtaacttta
     1561 ttataccaga gaagtaatca aattcaccaa aatcgcaaat ctgaaatttc tataagaaaa
     1621 aatatcaaat atgcgatttt ctaaaataag ccaattttcg tgttatactg tacttgtaaa
     1681 gcacttgaag caaatcctag gtcgcagaag tggtttacaa atgaagataa ttgaaggagt
     1741 gtaaaagatg ttaaatttac aatttgcaga aacaatggaa ttgacagaag ctgagttgga
     1801 gatagtttat ggaggggaat ttgggaataa tgctgttatc ccagccggtg cttggggagg
     1861 gttcgggaca ccttggtcaa tcactaattt ctggaagaaa aattttaacg atagacctga
     1921 ctttgattct gaccgccgtc gttattaata tccaaagccc ttgccttttc ttgggtaggg
     1981 gctgtcttta tttcatagta taggaggaaa tagaatgaaa aagataatct cacaccgcta
     2041 ctttattata gtttttctac tagttattgc tgaccagaag ttcagtgttc tagttttacg
     2101 cagcgacctt gttactgggc tatctgactt tgcctattat ctgtcggata tgatgttgaa
     2161 ttttcttgtg gttttatttg ctcttattgc tatgatttgg tcggggaaat ggcagaaaat
     2221 caatagtaga aagtttaaag gatcctatct tttctattcc ttcctagctc tgcttgcttt
     2281 tgttgtttgg aatttcgtta ccttttttct tttcccacct actcgaaatg aaatttctta
     2341 tcaacatgct gctccgactt ttacaggggc tacagcattt ttgatgtatt ttttctatcc
     2401 tgtgattgca ggtcccattt ttgaagagat aatccatcgg ggattggtga tgacagctct
     2461 ggaaaaagga aagaaatggg ggctagatgt acttggctct gctattttat ttggggtctc
     2521 gcacattagt aatcacggtt gggtcttgac agactttgtc ttctatatgg gtggaggtct
     2581 tatatttgca gtcttattta gaatgacaaa gtcaatttat tggcctattg gactgcatat
     2641 agtctacaat ggtattggtc agcttttgat gttactgtaa ttttggttgt tagtagtatc
     2701 gcggtctatt cttggggaaa gattcccatt tgaacttaag aaggagttgt aatgaaagta
     2761 tttcttcaaa atagagattt taggcaatta actatcaacc agtggatttc aacgcttggg
     2821 gatacgattt tttatctggc cttcctgaat tatgtggcag atgcgtcttt tgcccctttg
     2881 gcgattttac ttatcacgat ttcagaaacc cttccccaag ttctacaaat ctttctggga
     2941 gttttggcgg attttcaaca tcatcgtgtc ttgaaataca cagtcattag ttttacaaaa
     3001 tttttgcttt actctatagt gtccctatcg ctttcagggc agtctttttc cttgttgcta
     3061 gtaaccttta tttgtatgat taacctcttg tctgacacat tgagttattt ttcaggcgcc
     3121 atgctcactc cgattttcat tagaattatt gggcaagatc atctggcaga agctattgga
     3181 tttaaacagt caactgttag tttagtgaaa acaatcagta atatcctagg aggagtctta
     3241 ctaggtattc tatccatcca gtttatttcc ttactgaacg ctcttacctt tttaatcgca
     3301 tttttaggta tcctcttcat aaaaactgac ctcttgaaag tagaaaaaac gattagctat
     3361 caagaaggac tctctgtaaa atccttttgc cagcatttgc tccaatcatc gaaattgata
     3421 tggaatatga ataaggtgct cttggttttg tttattatct ctactagtca agcagtgata
     3481 aatgttacag ttcctgtctc tactctattt ttgaggaatc aacccttttt gaatttacaa
     3541 acaggtcaat ctcttgcctt gctatccact tttgaactgt cagccctgat tgtcggaagc
     3601 cttgtaagtg gctatctgca acataccatt tccataaaaa cagctctcta tgcctcactt
     3661 gtcatccagt tgcttcttct agtaggattt gccacagttc gttttgactg gattctcatc
     3721 tttagtacct tggatgcctt tttcgcaggt gttctctctc ctagattgca agaactcgtc
     3781 tttaaacaaa tacctgagga gtcaatggga gcagttcaat cttctatcag tgccattacg
     3841 gttgttttac ctagcttatt tacaatatct ttggtaacca ttgctactag ctttggaact
     3901 ctggcagtta gctttgtttt attgctattt cttctagttg cctttgttat gcttctgaat
     3961 attcgtgaaa gtatttagtg gagaatttct ataatatatg tttgagactg gattgccagt
     4021 ctttttatga tggtcaaaat caacagactt tttctctttt ctgaatgtgt tatagtagac
     4081 tgaatctaaa atagtacgaa acaattgcta aaacatttat agaaattaat tttactttcc
     4141 caatcgattt gttctcatct tatttcaatc tgctatataa tagtccatgc tgtggctgga
     4201 ttcttttcag cctacttata ctcttcgaaa ctctcttcaa accacgtcag cttcaccttg
     4261 ccgtatatgt gttactgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc
     4321 tgacttcatc agttctatct gcaacctcaa aacagtgttt tgagcaacct gcggctagtt
     4381 tcctagtttg ctctttgatt ttcattgagt attattaaga caatatgagg agaaagatga
     4441 aagaaaaagg attttaggag ggggcgcagg cagccatgcc aacggctctt ggctatgtca
     4501 gtatcggcct ggcctgtgga attatcggtg cgccctatgt gacacctgtt gagatgggct
     4561 tgatgagtct ctttgtttat gctgggagtg cccagtttgc catgttggca ctgattgtgg
     4621 ttcaagctcc tgtggcagct attgctatga cggtttttct aatcaatttg cgtctctttt
     4681 tgttgagctt acacgcatcg acttatttcc gtcataccag tctttggcac aatatcggta
     4741 tgtctagtat cttgacagat gagacctatg gcgttttgat gggcgaatta gcccatacag
     4801 ataaggtcaa tccgatgtgg atgcacggga acaatctcaa cagctatgtg gcttggtttg
     4861 tgggaacagt ggtcggaacg gctctaggtg acctgttgcc aaatccagaa atctttggtt
     4921 tagattttgc cctggttggg atgtttatcg ggatttttgc ttcgcaattc cagatgatgc
     4981 aaagacggat ttctgtccgc aatctgctca ttatcttagc agttgttgca gtgtccttct
     5041 ttttactttt gacagtggtg tctcagtcac tagctgttct gtttgcgacg ctacttggtt
     5101 gtagcatggg gatggtttta gatggtcagt aagtatcttt tattagcagt tattttctct
     5161 ggtcttgtga cctggattcc ccgtatgatt cccttcatct tggtcaagta taagggcctg
     5221 cctacgattg ttgagcgttt tttgaagttt ttgcctgttt ccattatctt tgccttgatt
     5281 ctttcaagag tagtgacagg taaggttggg aaccttcctc aaatcaaatg actggacttc
     5341 ttagcagtct ttccaacagc ttgggtagct tttcgctacc gcaatctagt cggtacggtt
     5401 ctctttggag tagtcttgat tgccgtcttg cgtttggtct tttaaataag tcataaagaa
     5461 aacttatcac agagatagat atcatataat ggcgtaatta ctttttttct gataagatta
     5521 taaggtattc tattttggag gaaatgacat gaaaaaaatc gttaaatact catctcttgc
     5581 agcccttgct cttgttgctg caggtgtgct tgcggcttgc tcaggtggtg ctaagaaaga
     5641 aggacaagca gctagcaaga aagaaatcat cgttgcaacc aatggatcac caaggccatt
     5701 tatctatgaa gaaaatggcg aattgactgg ttacgagatt gaagtcgttc gcgctatctt
     5761 taaagattct gacaaatatg atgtcaagtt tgaaaagaca gaatggtcag gtgtctttgc
     5821 tggtcttgac gctgaccgtt acaatatggc tgtcaacaat cttagctaca ctaaagaacg
     5881 tgcggagaaa tacctctatg ccgcaccaat tgcccaaaat cctaatgtcc ttgtcgtgaa
     5941 gaaagatgac tctagtatca agtctctcga tgatatcggt ggaaaatcga cggaagtcgt
     6001 tcaagccact acatcagcta agcagttaga agcatacaac gctgaacaca cggacaaccc
     6061 aactatcctt aactatacta aggcagattt ccaacaaatc atggtacgtt tgagcgatgg
     6121 acaatttgac tataagattt ttgataaaat cggtgttgaa acagtgatca agaaccaagg
     6181 tttggacaac ttgaaagtta tcgaacttcc aagcgaccaa caaccgtacg tttacccact
     6241 tcttgctcag ggtcaagatg agttgaaatc gtttgtagac aaacgcatca aagaacttta
     6301 taaagatgga actcttgaaa aattgtctaa acaattcttc ggagacactt atctaccggc
     6361 agaagctgat attaaataat ttcttgaaat catccattct gagtaagatg ggtggtttct
     6421 cagtttttag ggatatagtt ttaaactata tatctgacta tctaataaca atttgtgaaa
     6481 tcaaacaaat tgtgagatac tagtacggta ttatttttaa ggagaaagaa tcatgaaaat
     6541 caaaaaatgg cttggtctag cagcccttgc tacagtcgca ggtttggctc ttgcagcttg
     6601 cggaaactca gaaaagaaag cagacaatgc aacaactatc aaaatcgcaa ctgttaaccg
     6661 tagcggttct gaagaaaaac gttgggacaa aatccaagaa ttggttaaaa aagacggaat
     6721 taccttggaa tttacagagt tcacagacta ctcacaacca aacaaagcaa ctgctgatgg
     6781 cgaagtagat ttgaacgctt tccaacacta taacttcttg aataactgga acaaagaaaa
     6841 cggaaaagat cttgtagcga ttgcagatac ttacatctct ccaatccgcc tttactcagg
     6901 tttgaatgga agtgccaaca agtacactaa agtagaagac atcccagcaa acggagaaat
     6961 cgctgtacca aatgacgcta caaacgaaag ccgtgcgctt tacttgcttc aatcagctgg
     7021 cttgattaaa ttggatgttt ctggaactgc tcttgcaaca gttgccaaca tcaaagaaaa
     7081 tccaaagaac ttgaaaatca ctgaattgga cgctagccaa acagctcgtt cattgtcatc
     7141 agttgacgct gccgttgtaa acaatacctt cgttacagaa gcaaaattgg actacaagaa
     7201 agcacttttc aaagaacaag ctgatgaaaa ctcaaaacaa tggtacaaca tcattgttgc
     7261 gaaaaaagat tgggaaacat cacctaaggc tgatgctatc aagaaagtaa tcgcagctta
     7321 ccacacagat gacgtgaaaa aagttatcga agaatcatca gatggtttgg atcaaccagt
     7381 ttggtaataa gaaacaggga ggtgggagag aaaattccac ctcttgcttt tgtatagagt
     7441 atagattgta aagaagacta ttcgttcata gaaaggtaga gagaatatgg tttttcctag
     7501 cgaacaagaa cagattgaaa aatttgaaaa ggatcatgta gtccaacatt attttgaggt
     7561 tttgcgtacc ttgatttcta agaaatcagt ctttgcccag caggttggac tcaaggaagt
     7621 cgcaaattat ctgggtgaga ttttcaagcg tgttggagct gaagtggaga ttgatgagag
     7681 ctatacagcg ccctttgtca tggcacattt caagagttcg cgtccagatg ccaagacctt
     7741 gattttctat aaccactatg acactgtgcc agcggatggg gatcaggtct ggacagagga
     7801 tccttttacg ctttcggtcc gcaatggctt catgtatggg cgtggggttg atgacgacaa
     7861 gggtcatatc acagctcgct tgagtgcttt gagaaaatat atgcagcacc atgatgattt
     7921 acctgtcaat atcagcttta tcatggaggg agcggaggaa tcggcttcaa cagacctaga
     7981 taagtatttg gaaaagcatg cagacaaact ccgtggggcg gatttgttgg tctgggaaca
     8041 agggaccaaa aatgccttgg agcagctgga aatttctggt ggcaataagg ggattgtgac
     8101 ctttgatgcc aaggtaaaaa gcgctgatgt ggatatccac tcgagttatg gtggtgttgt
     8161 ggaatcagct ccttggtatc tcctccaagc cttacagtct cttcgtgctg cggatggccg
     8221 tatcttggtt gaaggcttgt acgaagaagt acaagagccc aatgaacgag aaatggcctt
     8281 gctagaaact tatggtcaac gaaacccaga ggaagttagt cggatttatg gattggagtt
     8341 gcctctctta caggaggagc ggatggcctt tctaaaacgt ttctttttcg agccagcgct
     8401 taatatcgaa ggaatccagt ctggttatca aggtcagggt gttaagacta ttttacctgc
     8461 agaagccagt gccaagctag aggttcgtct ggttccgggc ctagaaccgc atgatgttct
     8521 ggaaaaaatt cggaaacagc tagacaaaaa tggctttgat aaggtagaat tatactatac
     8581 cttgggagag atgagctatc gaagcgatat gagcgcacca gccattctca atgtgatcga
     8641 gttggccaag aaattctatc cacagggcgt ttcagtcttg ccgacgacag cggggacagg
     8701 acctatgcat acggtctttg atgccctaga ggtaccaatg gttgcattcg gtctaggaaa
     8761 tgccaatagc cgagaccacg gtggagatga aaatgtgcga atcgctgatt atcacaccca
     8821 tatcgaatta gtagaggagc tgattagaag ctatgagtag agatattatc aagttagatc
     8881 agatcgatgt gacttttcac caaaagaaga gaaccattac agcggttaag gatgtgacca
     8941 ttcacatcca agaaggggat atctacggaa tcgttggata ttctggagca ggaaaatcaa
     9001 cccttgtacg ggtgattaat ctcttgcaaa aaccatctgc agggaaaatt accattgacg
     9061 acgatgtgat ttttgacggc aaggtgacct tgacggcaga gcagttgcgt cgtaaacgtc
     9121 aagatatcgg aatgattttc cagcatttta acctgatgag ccaaaagaca gcagaggaga
     9181 atgtagcctt tgcccttaaa cactctgaac tcagcaagga agaaaagaag gctaaagtag
     9241 ctaagttgtt ggacttggtt ggtttggcag atcgtgctga aaactaccct tcacaactat
     9301 ctggagggca aaaacagcgt gtggcaattg cgcgtgcctt ggccaatgat ccaaaaatct
     9361 tgatttcaga cgagtcaact tctgcccttg atccgaagac aaccaagcag attttggcct
     9421 tgttgcaaga tttgaaccaa aaattaggct tgactgttgt cttgattacg catgaaatgc
     9481 agattgtcaa agacattgcc aaccgtgtgg cggttatgca ggatgggcat ttgattgaag
     9541 agggtagtgt gcttgaaatc ttctcagacc ctaaacaacc tttgactcaa gactttatct
     9601 caacagctac aggtattgac gaagccatgg tcaaaatcga gaagcaagaa atcgtggaac
     9661 acttgtctga aaacagtctc ttggtgcaac tcaagtacgc tggatcttca acagacgagc
     9721 cacttttgaa tgaattgtac aagcattatc aagtaatggc taatattctc tatgggaata
     9781 tcgaaatcct cgatggtact cctgttggag aattggtggt ggtcttgtca ggtgaaaaag
     9841 cagcgttggc aggtgcccaa gaagccattc gtcaagcagg cgtacagtta aaagtattga
     9901 agggaggaca gtaagatgga atcattgatt caaacctatt tgccaaatgt ctataagatg
     9961 ggttggtctg gtcaggcagg ctggggaaca gctatctacc taaccctcta tatgacagtt
    10021 ctttccttca ttatcggagg cttcttgggg ctagtggcag gtctctttct cgtcttgaca
    10081 gcgccaggtg gtgtcttgga gaataaagtc gtattctgga ttttagacaa aattacctca
    10141 atttttcgtg cgattccctt tatcatcctc ttggcaatct tgtcaccact ttctcacttg
    10201 attgttaaaa caagtatcgg gccaaatgca gcccttgtcc cactttcttt tgcagtcttt
    10261 gccttctttg cccgtcaggt gcaggttgtc ttggctgaat tggatggcgg tgtcattgag
    10321 gcggctcaag cgagcggagc gactttctgg gacatcgtgg gtgtttacct atcagaaggt
    10381 cttccagatt tgatccgtgt gacgactgtg accttgattt cccttgttgg ggaaacagct
    10441 atggccggtg cggttggagc tggtggtatc ggtaacgtag ccatcgctta tggatttaac
    10501 cgctacaatc acgatgtgac catcttggca accattatta tcattttgat tatctttgca
    10561 atccaattct tgggagattt cttgactaag aaattgagtc ataaataaaa agagccgcgt
    10621 ggctcttttt aactgatcag attttctgtg caaatttttt actcaaggct tgtccaatca
    10681 aggcacccac tagggctccg atgacaatac ttgcgataaa tagaaggaca gttccagggt
    10741 ttggagcgac catgatacgg tcgatatatt cttgggattt tcctcttgcc agaagagtag
    10801 ccatataggc tttgggcgca atccacataa gcaagattgg tcctgttgta ctaaaggcga
    10861 aaataatgaa agaaaggaag ttctttgttt tgtccttgta ttttcctaaa tgagctactt
    10921 catctgctag gaggccacag ataattccag gaaggaagga accagcaccg tgtttagttc
    10981 ccaagaaaaa gagggcaatg acaaggccga tagtggtaat ggctccaaag cgcggaactt
    11041 ttgcgactag gatcatatag acgctaccgc cgacaagggc agtaaaggca ggcgcataaa
    11101 acatgtttcc agtttggtca aagagattgc ccaaaaggac accaatcccc atgcagagga
    11161 agtaaagaac tgcaaaaagc agtgtagtta agatagattt cttcatgaat tgtctcctga
    11221 taattttttc acaattctca ttgtaccacg gtttggtggg attgtaaatg gatactagaa
    11281 gtttttgaaa acgcttgaaa tatgatataa tggtttcata gttattttta ggggaatatc
    11341 atgggtagat ttttagactt tgtctttaat cgtttctttt tagggatgat tgcgacagcc
    11401 ttcttttggc tattaacttt agcaggaggg attatccttg gtctagcgcc ggctagtgcc
    11461 accttgatga gcttatatgc agaacatggt tatagctttc gggaatacag tttgaaggag
    11521 gcttggtctc tttacaagca aaattttgtc tcaagcaacc tgattttcta tagcttttta
    11581 ggtgtgggtc tagttttgac ctatggtttg tatctcttgg tgcaattgcc tcatcagacc
    11641 attgttcatt tgattgcgac ccttttgaat gtcctagtag ttgccctgat ctttttggct
    11701 tatacagtat ctttaaaatt acaagtttat tttgccttgt cctatcgaaa tagtctcaaa
    11761 ttatccttga ttggcatctt tatgagtcta gcagctgtgg ccaagattct ccttgggact
    11821 gtgctacttg tagcaattgg ttactatatg cctgccctgc tattttttgt aggaattggg
    11881 atgtggcatt tctttatcag tgatatgttg gaacctgtct atgaaatcat ccatgaaaaa
    11941 ttggcgacaa aatagaatga agcagttttg gctacatacg cttctaagaa cctatagttc
    12001 agtgatgatc attatcattg cgagttttgc aatcttactc tcttacgctg actgggattc
    12061 acgtgaaaag gaagcccaga gagtagccca gcgtgtaact gctagaacag tgagtgaaat
    12121 tgaatattac catagagagt caacccagat agctcaggct ttagttgaaa accaagctcg
    12181 tattgaggga atctataaat actttagtct tagcatgcca gactattttt actggcaatt
    12241 agagcggaaa gcttcgcctt atatatcagt ctctctgtat gaaaatgttg atgacctcta
    12301 tgttcgaaat gattttgtaa ctggagtggc cattgctttt caagattaca aggaagtcta
    12361 tgtttctact aaagacaaac gtagtggaga aaaaatcagg gctgaggatt tcaaaccagc
    12421 aggaaatagt tttgccattc cggtgtcaga tccagtgtca gatcaagatt taggagtgat
    12481 ttacatctcc ttggatcctg ctgttttata ccatgccatt gataatacta gaggtcatac
    12541 tccgatggca gtaacagtga cctcaccttt tgatacggag atttttcata ttggtgagac
    12601 agttgataag gagagtgaaa attggctagt tggcttaact tctcatggat atcaggttca
    12661 ggtggcagtt cctaaaaact ttgttttaca aggaacagtg actagctctg ctttgattgt
    12721 gggtttgagc cttctcttta ttgtcattct ttatctgact ttgaggcaga cttttgctaa
    12781 ttaccaaaag caggtagtgg atttagtaga atccattcaa gtcattgctc aaggcgaaga
    12841 ggggcgtcgg attgacattt ccgagaaaga tcaggaatta ctcctaatcg cggagacgac
    12901 caatgatatg ttggatcgat tggaaaagaa tatccatgat atttaccagt tagagcttag
    12961 tcaaaaagat gccaatatgc gagccttgca ggcgcaaatc aatcctcatt ttatgtataa
    13021 tacgctggag ttcttgcgca tgtatgcagt tatgcagagt caagatgagt tggcagatat
    13081 catttatgaa ttcagtagtc tcttgcgtaa caatatttcc gacgaaagag agaccctcct
    13141 caaacaggaa ttagaatttt gccgtaaata cagctatctc tgcatggttc gctatcccaa
    13201 gtccattgcc tatggtttca agatagatcc agagttagag aatatgaaga ttcccaagtt
    13261 taccttgcaa ccgctggtag aaaactattt cgcgcatggt gttgaccaca ggcggacaga
    13321 taatgtgatt agcatcaagg ctcttaaaca ggatggtttt gtggaaattt tggtggtcga
    13381 taatggtaga ggaatgtcgg ctgaaaagtt ggcaaatatc cgagaaaaat taagtcagag
    13441 atattttgaa caccaagcca gctacagtga tcaaaggcag tctatcggga ttgtcaatgt
    13501 acacgagcgt tttgtgctct attttggaga ccgctatgcc attactatag agtctgcaga
    13561 gcaagccggt gttcagtatc gtattacaat tcaagatgag tagaaaggga gaaaatgtat
    13621 aaagtattat tagtagatga tgagtacatg gtgacagaag gtctgaagcg tttgattccc
    13681 tttgataagt gggatatgga ggttgtcgca acagccagtc atgccgatga agctctagaa
    13741 tatgttcagg aaaatcctgt cgatgtcatc atttccgatg tcaatatgcc agacaaaaca
    13801 gggcttgata tgattcggga gatgaaagag atcttaccag atgctgccta tatcctgctc
    13861 tcaggttatc aggagtttga ttatgtaaaa agagcaatga accttagtgt ggtggactat
    13921 ttggtcaagc ctgttgataa ggtagagctg ggaaatctgc tggagaagat tgcaggtcag
    13981 ctcggcgaga gagggaagaa aagtcagact cttagtcaag aattagacga ggctggattt
    14041 gttagttatt taggggataa ggagaattgg tggataggtc tatccaagga aaaacaaggt
    14101 tccttcacca ttccctacta tgtcttgggc caagactggc agattttcat ttctggccaa
    14161 cctctagatg gtttagtcgt tacacctttt gaagctcctt atcaagaaca ctttgaacgc
    14221 tggaagctga atgctgagaa aaccctcttt tacggttctg taaatttgca gcagtctgag
    14281 agtctctttg cctattacga accgatttat agggttatca ttcagggaaa tctcaatcaa
    14341 atcgtagaag agttaaatct cttggagaag gtagttcttg aaaatacgcc gcgtgttccg
    14401 attactaaac agctttttat tcagtttgtc atggatgttt tccatttatt tgaacatctc
    14461 aaagctgatg acctgacaga catcgtcaaa accattcatg ctattcaatc cttcgatgaa
    14521 ttggtttctt atatcaagga aactctgatc agctttttcg gtcaataccg tatgaatgaa
    14581 aatgtggtca gtgtgctgga agtcattggt cgtgattacc aaaaagagct ttccctcaag
    14641 gatatcagta aggctctctt tatcaatcct gtttatctag ggcagttgat taagcgtgaa
    14701 accgattcga cttttgcaga gttactaaac aaacaacgta ttaaggctgc ccaacaactt
    14761 ttgctttcaa ctagtgacag catcgaagat atttgttatg ctgttggtta cagtaacctt
    14821 ggatatttct ataaagtttt ccgaaaattg tgcggaaaat cgccaaaagc ctaccgaaaa
    14881 caggtagaaa ctatactata agatttgtat tcctttacaa aaggtgctat aatattatca
    14941 ggaggttcta tcatgaaaaa gaaaccaatt tatctatggg tcttgctaat cttgtctgcc
    15001 cttatttcag ttccgtctct gtttggaatt gtgagtccct tgcctagtaa agaagccctt
    15061 cgtgctgctc agaaacaagt tgcaggggtc aatgctcagc aattagaaga ccagcttaat
    15121 tatacctata gagtagcaga agcatctcat tctattttta atgttgcttt gattgtgcta
    15181 tctactattt tagtggtcgt agcgattgtc ttccttgttc gtaaaaattt gcaatatgca
    15241 aactatactt acgttggcta tgttttgtta gctattattg gttcgattta tggctatgtt
    15301 ggtttacaag acgctgtgca gttggttcaa gatgagagca tgcgtttgac agtgagtatt
    15361 ggttcaaaag ctgttagcat tttctatatc gtcatcaatg ttctcttcct agcccttgtc
    15421 ttctataaga tgtggcgaca acagaaagcc ttggcagaag aagaggaaac agaagagctt
    15481 acctaagtat tgacaaaaaa gaactatcaa gatacaatct tggtagttgt tttttgatta
    15541 tagataaaat catggaggta catatgaaga caatttcttt agtttatatt agtttgagtg
    15601 gcaacactga aagttttgtg acgcgcttga aagactatct cttgtcccag tacaaaggga
    15661 ttgaggttca aaagattcat atcaaggatt tggtcaagga aggcaaaaat ttctatgaaa
    15721 tggaccatcc ctatgtcgct tttttgccga cctatctcga aggtgggaat ggcgtggata
    15781 acggagatgt tgagattttg acgacaccgg tgggagattt tattgcctat ggtaacaatg
    15841 ctagtaagtg ttttggtgtg gttgggtcag gaaatcgtaa ctttaataac caatactgcc
    15901 taacagccaa gcaatacagt caacgctttg gcttccctgt attggctgac tttgaaatgc
    15961 gaggcatgct ggaagatatc aaacatgtcg cagctattat cgcagatttg tatgagttgg
    16021 aaaaggagaa ttaatactca atgaaaatca aagagcaaac taggaagcta gtcgcaggtt
    16081 gctcaaagca cagctttgag gttgcagata gagctgacgt ggtttgaaga gattctcgaa
    16141 tagtataaag ttcttataag atgtggttga agttgttcaa ctgttttttg agtataaaca
    16201 gtctttaaga acgtaaaaaa gcataagttc aggtcttcaa aaccttgttc ttatgctttt
    16261 tatcatagaa atatttgctg aattttctct tgaaatgaaa ccttatcttc atctcgcgct
    16321 tgtcccttac cctgcttttc cgattgcgag tgcgtagata aagaagatgg cgaagccaaa
    16381 gaggaaaatc aatcccgcaa tggcaaggtg tttgagccaa gaaggaagat ttttgttttc
    16441 acgtgttacc aaggcgtaat tcaaaagagc aaagaaaggt gttgtcagga aagagccaat
    16501 catggcaaag cggagcatgg ttgaaacctg accagcgaag aacttgataa tgacgatacc
    16561 gatgatagca gtgatggtca tccagatgtt caaagattta cgattgtcct ctttttgact
    16621 gattagcagt cggagagatt cctgattaac gcgagaatag ccatcgataa cagttataac
    16681 tgttccaaag atacagagga aggcaataaa ggtaatcaag taacgggacc attcgccaag
    16741 aacagaggca tacatgccca cgaattgaga gatgtatttg gctgaagcag cttcaaccgc
    16801 ctgccctgta ggatactgaa tcagtgctcc cagtgccaca aagaagacgg ctaggatagc
    16861 tgttccaata taaccagtgt taaagtcaaa cagagcgtct tctgtgttaa agttgacggt
    16921 ctttctcttt tcagctgacc aaagtgaatt gatggctgaa atttcaatag gagccggcat
    16981 ccatcctagg agggagacga tgaagggcag agctgccatt tgccaaggtg tcttctcgac
    17041 aaaatcagaa ctgtattctg gatgcttgac cgccgcaatg ataactgcaa gaacagtcgc
    17101 aatggttaag gcagacataa tccatttgac catgccgtct aaaagtttgt agcctccaaa
    17161 gagtagcata gcccaaatga ttgcaacgag aatgagggac cactgagtaa tgctaagtcc
    17221 aatcattggg aaggcactgg cgatgatagc tgagcacaga atggcaacac cagccgtgtt
    17281 gaccatagcc gaaaagacat tgagaataaa gaaaatccag agatagagtt ttcctttttc
    17341 ggcataacct tcaaccaaag tctttccagt atcagctgtg tattcagcac caaaacggaa
    17401 aaatggatat ttaaagacat tggctaagat gaccaagaga agtagagacc aaccgtaaga
    17461 accgccagct tgagttgagg atacaatgtg ggaacctcca acagcggcag ttgccattag
    17521 gattccaggt cccattgctt ttagcttgct tgcccaggtt gattgattta aggaaatagc
    17581 ttgcgacatg ataaatactc ctttttgaat tttcagaata atctgtatat gcaatctata
    17641 ttctacaaaa aattttggaa aaatgcaaga atattttgaa aaaagataga aaatttacaa
    17701 agaaaatctg aaataactgt tatgatatag cagattgaaa ctagaatagt acacttttgc
    17761 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatt gatttatcct gttcttattt
    17821 catttcacta tagtaaggtg cagcaggata aagcaaaacc gagaagtttc tttctcggtt
    17881 ttgcttatta tgaggttcct attttctatg ctccaagagt tgattgatgt gatctacttt
    17941 tttagcctct cttttataga gaatagccca gatgatgaga tagacaatcg caaactcgat
    18001 aatgagctgg aggtagaaaa tccagtggaa ggggaaccaa ccagcaagag ttgctagtgg
    18061 gacaaagcca gccagcataa ggaagaaatg agtcagagtg gcacgaagca tgctccagtc
    18121 acggctgaat aagcggttgc caaagttgaa gagcataccg atggctgccc agataagtgt
    18181 gcagtagagc aagaccaggg caccgtgaac ctgatgctga tccatcactt ggccgatgag
    18241 agagtaggga tttagtggtg cgtaggtatt tggtgcataa atgagtgaaa agaggatgga
    18301 gaggatgagg ccgataagaa caccggcagc tgcatcgtga aatacttgtt ttttcatagt
    18361 tctaatttct ccttgatggt ttttagataa cggcgtgaag agtaggtgaa gctttcgttt
    18421 ttcaagaaaa tttctaccag accgtttggc gtgagcttga ggtgagagat ggaatcgata
    18481 ttgatgattt ctgattggga aatttggata aaattgcttg gcaagagttt aagaacctga
    18541 tagagtcgca aatcaatgct gtaggtctga ctcgcggttt ctgctagaac cttccgattc
    18601 tcgatataga agcgctgaat cttgccaatc tcaactagat agacctgatc atcgattttt
    18661 cctttgattt tttctctttg gtccagattt tctgcgaact cgatgacttt ctggactttt
    18721 tcggtttctt gaggtgcttg gacaatcagc ttttcctcct cgtaagtctc actaatctgt
    18781 agttctactt tcataaattt ctctccttgt ttttcataca aaattaatct cacttcttgt
    18841 acacctttat taatactctt caaaaatctc ttcaaaccac gtcagcgtcg tcttgccgta
    18901 ggatagttgt tgactttgtc agttttatct acaacctcaa aacagtgttt tgagctgtct
    18961 gcgtctagct ttctagtttg ctctttgatt tttattgagt ataccactat tttactccct
    19021 ctggcaaggg actttgtcta tgtggaggga ttgggctcct atgtggtgga gtttttctgt
    19081 tctttctgaa atatggtata atagcactaa ttaatttcta ggaaaataga tacagaaagg
    19141 ggctgaaaga tgtctcatat tattgaattg ccagagatgc tggcaaacca aatcgcagct
    19201 ggggaggtca ttgaacgtcc tgccagtgtg gttaaagagt tggtagaaaa tgccattgac
    19261 gcgggctcta gtcagattat cattgagatt gaggaagctg gtctcaagaa ggttcaaatc
    19321 acggataacg gtcatggaat tgcccacgat gaggtggagt tggccctgcg tcgccatgcg
    19381 accagtaaga taaaaaatca agcagatctc tttcggattc ggacgcttgg ttttcgtggt
    19441 gaagccttgc cttctattgc gtctgttagt gtcttgactc tgttaacggc ggtggatggt
    19501 gctagtcatg gaaccaagtt agtcgcgcgt gggggtgaag ttgaggaagt catcccagcg
    19561 actagtcctg tgggaaccaa ggtttgtgtg gaggatctct ttttcaacac gcctgcccgt
    19621 ctcaagtata tgaagagcca gcaagcggag ttgtctcata tcattgatat tgtcaaccgt
    19681 ctgggcttgg cccatcctga gatttctttt agcttgatta gtgatggcaa ggaaatgacg
    19741 cggacagcag ggactggtca attgcgccaa gcaatcgcag ggatttacgg tttggtcagt
    19801 gccaagaaga tgattgaaat tgagaactct gacctagatt tcgaaatttc aggttttgtg
    19861 tctttacctg agttgactcg ggctaaccgc aattatatca gcctcttcat caatggccgt
    19921 tatatcaaga acttcctgct caatcgtgct attttggatg gtttcggaag caagcttatg
    19981 gttggacgtt ttccactggc tgtcattcac atccatatcg acccttatct agcggatgtc
    20041 aatgtgcatc caactaagca agaggtgcgg atttccaagg aaaaagaact gatgaccctg
    20101 gtttcagaag ctattgcaaa tagtctcaag gaacaaacct tgattccaga tgccttggaa
    20161 aatcttgcca aatcgaccgt gcgcaatcgt gagaaggtgg agcaaactat tctcccactc
    20221 aaagaaaata cgctctacta tgagaaaact gagccgtcaa gacctagtca aactgaagta
    20281 gctgattatc aggtagaatt gactgatgaa gggcaggatt tgaccctgtt tgccaaggaa
    20341 accttggacc gattgaccaa gccagcaaaa ctgcattttg cagagagaaa gcctgctaac
    20401 tacgaccagc tagaccatcc agagttagat cttgctagca tcgataaggc ttatgacaaa
    20461 ctggagcgag aagaagcatc cagcttccca gagttggagt ttttcggaca aatgcacggg
    20521 acttatctct ttgcccaagg gcgagatgga ctttacatca tagatcagca cgctgctcag
    20581 gaacgggtca agtacgagga gtaccgtgaa agcattggca atgttgacca gagtcagcag
    20641 caactcctag tgccctatat ctttgaattt cctgcggatg atgccctgcg tctcaaggaa
    20701 agaatgcctc tcttagagga agtgggcgtc tttctagcag agtacggaga aaatcaattt
    20761 attctacgtg aacatcctat ttggatggca gaagaagaga ttgaatcagg catctatgag
    20821 atgtgcgaca tgctcctttt gaccaaggaa gtttctatca agaaataccg agcagagctg
    20881 gctatcatga tgtcttgcaa gcgatctatc aaggccaatc atcgtattga tgatcattca
    20941 gctagacaac tcctctatca gctttctcaa tgtgacaatc cctataactg tcctcacgga
    21001 cgtcctgttt tggtgcattt taccaagtcg gatatggaaa agatgttccg acgtattcag
    21061 gaaaatcaca ccagtctccg tgagttgggg aaatattaaa agtataaaaa agtctgggaa
    21121 aaattttcaa aatcagaaaa acgcataaaa tcaggtgttc aaaaaccttg attttatgcg
    21181 ttttatcatg gaaatagtta cttcatgttt tcctaattct tttcgaaact ctttttaaac
    21241 cacgtcagtt ttatcagtaa tctcaaaaca gtgttttgag ctaattttgc cagttttgtc
    21301 tgtaacatcg aagttgtgtt ttaccactct gcgactggtt tcctagtttg ctctatgatt
    21361 ttcacagagt attaaattgc gattttgcca agtttcttta ttcgtctaaa agtagagtct
    21421 gttctatgcg tctaatgtac gaattaggtt gaccatttca atagctcctt gtgcacactc
    21481 agaaccctta tttcctgctt tggtaccagc tcgttctatg gcttgttcaa ttgtatctgt
    21541 cgttagcaca ccaaacataa caggaatttc gctatttaaa ctgatttggg cgattccctt
    21601 agatacctcg ctacatacat aatcataatg acttgtattc cctctaatga cagctcccaa
    21661 gcagataatt gcatcatatt ttttactttt tgccattttt gatgcaatca gtggtatttc
    21721 aaaagctcct ggaacccagg ctacctctat atctttctcg tttacattct ctcttttgag
    21781 attatctagt gctccagata ataattttga agttataaat tcattaaatc tcgctacaac
    21841 aatacctatt ttaatattgt ttgctactaa attaccttca taagtgttca tttatttttc
    21901 ctccatattt aaaatgtgac ccattcgatt tttctttgtt tctaaataaa aactatcgta
    21961 aggattggct tctatttcga ttgatattct actggaaatg gtaattccat atttttctaa
    22021 ctgttcaacc ttgtcaggat tatttgtcag taaatgaagt gactgaagtc ccagatcttt
    22081 aagcatttgt gctccaatat gatattctct taaatcacct tcaaagccta atgcaagatt
    22141 ggcatcaagc gtatccatgc cttgatcttg taaatgatag gcttttaatt tattgataag
    22201 tccaattcct cgtccctcct gtcgcaagta aagtaagaca cccgaaccat tctcaacaat
    22261 cattttcata gctttatcga attgctgtcc acaatcgcaa cgtaaagagc ctaaaacatc
    22321 tcctgttaaa cattcggagt ggacccgaca taatacattg gcttcatcct ctatgtttcc
    22381 cataataaga gcaagatgat gttccccatt tagtttatct atatagctaa ttgctttgaa
    22441 attaccgtat ctagtaggca tattgacagt tgaaactcgt tctatcagct gatcatatac
    22501 ttttctatat tcttgtaatt ctttgatggt aattagtgga atgttgtgtt tcttcgagaa
    22561 ctgaattaaa tcatctgttc tcatcatttt gccatcatga ttcattattt cacaacatag
    22621 gccacactct tttagtccag ctaattttaa taaatcaaca gttgcttctg tgtgtccatt
    22681 tctttctaga acaccacctt tttttgcaat taaaggaaac atgtgtcctg gcctgcgaaa
    22741 atcagagggt gttatatctt cagctacaca catacgtgcg gtcagtcctc tttcctcggc
    22801 agaaatacct gtggtcgttt ctttataatc aattgaaact gtaaaagcag tcttatgatt
    22861 atctgtattg ttttcaacca taggtgaaag cattaattga ttagctaaac tttcgctcat
    22921 aggcatacaa attaatcctt tggcataagt agccataaaa ttaacatttt ctgttgtagc
    22981 tgcttgtgca gaacaaatta agtctccttc attttctcta tccttgtcgt ctataacaag
    23041 aacaagtcgt cccttctgca atgcttctaa tgtttcttgt atttttcgat attccattga
    23101 ctgattatcc tttctgctaa aatccatttt gatataatag ttccttagat atttctgatt
    23161 ttggagagtt atccatcagt ttttgcacat atttacctaa gatatcattt tcaagattta
    23221 ctgtactccc gacttgttta cttttaagaa tggtttgttt caaggtatga gggataacag
    23281 atactgaaaa gtttactttg gagactttag cgacagtcag actaatgccg tcaattgtaa
    23341 tagatccttt ttcaactatt aaatctaaaa tttctttttg tgtgttgatt tgataccata
    23401 cagcattatc atcttttttt attgacgaga tttttcctgt accatcaatg tgtcctgtaa
    23461 cgacgtgacc cccaagtcga ccgttgatag ataaggctct ttctagattc acctcacttc
    23521 catgttttaa tagagtaaga gctgttcgac tccatgtttc attcattaca tcaactgtaa
    23581 aggattgatg attgaaatga gtaactgtaa gacagatacc atttactgct atactatcgc
    23641 ctaaatggat atccgttaat atttttgagg ctttaattga tagtttacaa ttacgagagt
    23701 ctttctgtat tctttcaact tttccgattt cttcaattat tcctgtgaac atggataaat
    23761 cacttcactt tctatgagat agtcatttcc tatttgagaa aaagcataag gtttcaatct
    23821 aatagcgtca tttggcaaag aaatgccttc acctccgaca ggaaacttgg cactgcctcc
    23881 aaaaattttt ggtgcaatat atattttcag ctcatcaaca atttgttgtt ccaaagcact
    23941 ccaattcatt agactgcccc cttctagaac taggctatca atctgcatgt ttcctagatg
    24001 ttgcattaaa ctcgataagt ctatatgatt gccttttttc tttatggaaa gtatttcaca
    24061 gccatgattt tgatatagct tcattttatt tttgtcttca gaggaagtgg caatgtaagt
    24121 tttaatatca tttgctgttt ttacgatttt agaggtaaga ggagttcgta aatgtgtatc
    24181 gcatatgata cggataggat ttttcccttc ctccaatcta catgtcagca aaggatcgtc
    24241 ttgaataaca gtattgactc ccaccataat tgcactaaca tggtgtcgta actgatgcac
    24301 atgctttctt gcttcttctt cagtaatcca tttggattga tttgttttag tggctatttt
    24361 tccatccatt gacattgcat atttcataaa aacatagggt acatgctggg taatatactt
    24421 tctaaaactt tttattaagt taagacactc attttctaaa attccaacag taacttgaag
    24481 attattttcc tcaagtatct ttactccttt tccagataca ataggattac agtctaggct
    24541 tccaatgact actcttgtaa taccactatc gattatagca tctatacagg gaggtgtttt
    24601 cccgaagtga caacagggtt caagtgttac ataaagcgtc gctccgacag gggattctct
    24661 acagttttta agagcatttc tctcagcatg tgggccacca aaaaactcat gataaccttg
    24721 tccgatgatg tgattatctt ttacaataac tgcgccgacc ataggattgg gattgacgta
    24781 accagcccct ttttgtgcca gttttattgc taatttcata tattttgaat cgctcatctc
    24841 actacctcca aaaaaaatat accttgaata ggggactact caaggcatac aaaagaaaac
    24901 ttatgcgatt aacaaaaata ctctgaaatg acaagtaatc atttcagagc acgcaaaaag
    24961 cacaaatata cttttatctt ctttcatcca gactatactg tcggctttgg aatttcacca
    25021 aatcatgcct ttcggctcgt gggctatacc accggtaggg aattacaccc tgccctgaag
    25081 atagttattc aattacagat gattatagta cttaattttg aatatgtcaa cagataaata
    25141 ccgattgttt ttgatatact gtatttgtga taatcgattc tcgctcctcg gataaagaaa
    25201 atatgatata ctagataaac gaaataagag agaaggaata ctatgtacgc atatttaaaa
    25261 ggaatcatta ccaaaattac tgccaaatac attgttcttg aaaccaatgg tattggttat
    25321 atcctgcatg tggccaatcc ttatgcctat tcaggtcagg ttaatcagga ggatcagatt
    25381 tatgtgcatc aggttgtgcg tgaggacgcc catttgcttt atggatttcg ctcagaggat
    25441 gagaaaaagc tctttcttag tctgatttcg gtctctggga ttggtcctgt atcagctctt
    25501 gctattatcg ctgctgatga caatgctggc ttggttcaag ccattgaaac caagaacatc
    25561 acctacttga ccaagttccc taaaattggc aagaaaacag cccagcagat ggtgctggac
    25621 ttggaaggca aggtagtagt tgcaggagat gaccttcctg ccaaggtcgc agtgcaagca
    25681 agtgctgaaa accaagaatt ggaagaagct atggaagcca tgttggctct gggctacaag
    25741 gcaacagagc tcaagaaaat caagaaattc tttgaaggaa cgacagatac agctgagaac
    25801 tatatcaagt cggcccttaa aatgttggtc aaataggagc agagaatgac aaaacgttgt
    25861 tcgtgggtca agatgacaaa cccgctctac atcgcctatc atgatgagga gtggggtcag
    25921 cccctccatg atgaccaagc attgtttgag ttgttgtgta tggaaaccta tcaggcaggc
    25981 ctgtcttggg aaacggtact caacaaacgc caagctttcc gagaagcctt tcatagctat
    26041 caaattcact cagtcgcaga gatgactgag actgaattgg aagccatgct ggagaatcca
    26101 gctatcattc gaaatagagc caagcttttt gctacacgcg ctaacgccca agtccttcta
    26161 cagttacagg cagagtacgg ctcttttgat gcctatcttt ggtcttttgt tgaggggaaa
    26221 attgtcgtta acgatgttcc tgattatcgc caagcgccag ctaaaacacc cttatctgag
    26281 aaattagcca aagatctcaa aaaacgaggc ttcaagttca caggcccagt cgccgtattg
    26341 tcttttctac aggctgcagg gctagttgat gaccacgaga atgattgtga gtggaaaggt
    26401 cttaaatgat gtctaacaaa aataagggaa ttctgatttt tgcgattctc tatacagtcc
    26461 tctttgtgtt tgatggcgtt aaattgctgg cttctttaat gccatctgtc attgcaaatt
    26521 atcttgttta tgtagtttta gctttatatg gctccttctt gttcaaggat agattgatcc
    26581 aacaatggaa ggagattaga aagactaaaa gaaaattctt ctttggcgtc ttaacaggat
    26641 ggctctttct cattctgatg actgttgtct ttgaatttgt atcagagatg ttgaagcagt
    26701 ttgtgagact agatggacaa ggtctaaatc agtctaatat tcaaagtacc tttcaagaac
    26761 aaccactact gatagctgtt tttgcttgtg tcattggacc tctggtagaa gaattatttt
    26821 tccgtcaggt cttattgcat tacttgcagg aacggttgcc aggtttacta agcattattc
    26881 tggtaggact tgtttttgct ctgactcata tgcacagttt ggccctatca gagtggattg
    26941 gtgcagttgg ttacttaggt ggaggccttg ccttttctat tatttatgtg aaagaaaaag
    27001 agaatatcta ctatccccta cttgttcaca tgttaagcaa cagcctctcc ttaatcattt
    27061 tagctatcag tatagtaaaa tgaaatgaga acaggacaaa tcgatttcta acaatgtttt
    27121 agaagtagag gtgtactatt ctagtttcaa tatactgtaa tatgtgatga aaatgccagt
    27181 aatgataccg agaaaaaagc tgagaaactt ttcccagctt tatttgttat agtcaaagag
    27241 aatgacttgt tcctgtgcat ctacatgagc atggacccca aagggtacaa ttgctcttgg
    27301 agttgcgtgg ccgacattca gattatagac aatcgggata ttgctgtcaa tgatatccaa
    27361 tagtgcctct ttatagtcgt catggaaagt ttcatccata ggttttccga ccaagagtcc
    27421 attgatgacc gcgaatatgc cagtgtcctt taaagttagc aacatctttt tgaagtcttc
    27481 tggcttaggc ttttcttcgc ttgtttcgag caagaggatt ttcccttccc agtctgacaa
    27541 gtcagggaaa agtttgtatt tttggcagag ttccgtgcta tctgcgtatc gagagttgtc
    27601 aaagatatcg tagagggatt cgaggcaacc accgaggatt ttcccctcga actgggcact
    27661 tccttgcaac aagtcaaaac ctgtatttgt atgactgaca cgaggtgttc ccagggccgt
    27721 gggactaaaa tcagttcgtt cctcatacca aacgtcacta gggcggattt ctgaaattct
    27781 tcccgtctca atcaattctt taaagtagtg aaggctatag gctagcattt ctttgtctaa
    27841 ttcacaaatg tctgctaaaa aggattgacc ataaaaagtc ttgattccta atttatgcaa
    27901 catgaggtgg ttcatggttg tatccgagaa gccaagaaaa attttttgtt tgataacctt
    27961 ttggagttgg tcattttcaa aaagataagg tagcaagcga taggtatcgt ctccaccgat
    28021 ggcacatagg atcatgtcga tgctatcatc agaaaaggca tgaatcaaat cctctgcacg
    28081 agcttcagga tggtccttga taaagtctaa tccttttaac gaatggggca aaaagatggg
    28141 attgagtccc agatccttga gacgttggat acccaagtcc acttcgtgtt tgacaaaatc
    28201 ctctccgata atgccactag ataaactaac aataccaata gtagaaacca tatctcatcc
    28261 tcctagaaat agattgagcc tattttatca caaaatataa gagaaagcta tgtgggattt
    28321 cagaagaata cttttaatac tcttcgaaaa tctcttcaaa ccacgtcagt tttatttgca
    28381 acctcaaagc tgtgctttga gcaacctgca gctagcttcc tagtttgctc tttgattttc
    28441 attgagtata actcaaaaaa gtaagtgaaa aagcaaccgc acctgcagtt gcttttatcg
    28501 ttttcttatt cttgatagcg tgttggtcct gcacttgcac tacggatatt caagcgtttc
    28561 ttgagataga agcgaagggc taggagggct actccaataa tagctagtgg caatggatcc
    28621 agtactgggt taaggctagc tggtaggaag cttgttgcaa agaagacaag caaccaaagg
    28681 aacatagaag ttaggataac tagtacagat ttccagaaag gtggacgttg actgcgatcc
    28741 atatctggtc catagtattg gtaaacaaag tagtacatca agtagaaggc aaatccacca
    28801 accagtccaa ctaatagaag agtaatcaat ccatagccga aagcttgatc tgccgcaaag
    28861 aaggttgtga gggcgctgac aagggcaaag aggctagtga tgaaaagagc tgagtccata
    28921 atcatcagtt ttggatcatc attttctttt ggatgctctt tttcgtactg ctctttgaca
    28981 gtgaagctat gagcccaatg agttggtgcg ccgtataggg aacgggcagt tgtaccttta
    29041 gattgctcct caaggatttg gggaatgact tcctcaaaaa tagtctggat ttcagcgtct
    29101 gttttcccat ctttgatgaa ttgttgggta gcaatgtgga caaactcttg gtttttctta
    29161 gttaattttt gtagatcaat ttgagacata ggaactcctc ttagaaccat tttttatgga
    29221 tgagatagag agtgagcgag acactcatag caaaggcgat aaagacgatt aaccagaagg
    29281 catttggctc tccgtttagg ggaatttcat tatccttaaa gttcatcccg taggcagaaa
    29341 agaccatggt tgggatggac atgacgatgg tcacaagggc caaggttttc atgatgttgt
    29401 tctggttgtt agaaatgata gaggcaaagg tctctgtcat agaatgcaag acgtttccat
    29461 aaatatctgc catctcgatg gcctgttggg tttcaatcag ggtgtcttca agcaggtctt
    29521 cgtcctcaag gtatttcttg atattgctgg ttgaactggt caatttctta atcacgcgct
    29581 catttgtttt gagggaggcc ttgaaataga cgatagtttt ttccaattcc atgagctcaa
    29641 tcaattcttc attacgagtt gattgatgca gttgactttc gatttgttca ctcttgcggt
    29701 cgattgaacg aagggctgtt aggtaaagct ctgcattgcg ataaagaatt tgaaagataa
    29761 aacgtgaacg catgaaggta tagaaattac gcaatcgacg gttgataaag acatcaagga
    29821 caggtagtgg ttccaaacac gtagtgataa tggtttcctc agtgatgata ataccaagcg
    29881 ggatggttac gtagtaggtg cggttatttc tttcctccgt gaccggcacg tctacgataa
    29941 tcagggtata ctcgtcttca atggtaatac gagacatttc ttccgcatcg agcggtgctc
    30001 gaaggtcggc aatatcaata tcgaaggtgt tggcgatttc gagtgattca ttttgagtcg
    30061 gattgacgag attgatccaa gtacccggtt caagcgtatc gatctcttta aattcagttg
    30121 ttgtagagag aaaaacttgt ttcatatccc ttatcctttc tcatttttca gattttttca
    30181 cactgtacta ttatactaca aaatcggcct tttgggtaat agaatctcac tttttttggt
    30241 ataatggtaa gcaataatgg actagaaaga acaaagatgc aagataaaat tgtcattcat
    30301 ggggcgcgtg cccataattt aaaaaatatt gatgtggaga ttccgcgaga caagttggtt
    30361 gtcgtgactg gcttgtcagg ttcagggaag tccagtctgg cttttgatac tctctatgcg
    30421 gagggacaac gtcgctatgt agagagtttg tctgcctacg ctcgtcagtt cttgggaaat
    30481 atggaaaaac cagatgtaga tgccattgat ggtctcagcc cagctatttc catcgaccag
    30541 aaaacgacta gtaaaaatcc tcgctcgacg gtgggaacga cgactgaaat caatgactat
    30601 ctgcgtctcc tctacgcacg tgtggggacg ccttactgta tcaacggaca tggagctatc
    30661 aatgcttctt ctgtggagca aatcgttgat aaggttttgg agttacctga acgccagcgc
    30721 ttgcagatct tggctcctgt catccgcaag aaaaaaggcc aacataagag tgttatcgag
    30781 aaggttcaga aagacgggta tgttcgtgtt cgtgtggatg gggaagtcta tgatgtgacc
    30841 gaagtgccag agttgtctaa gagcaagcaa cacaatatcg atgtcgtggt tgaccgtatt
    30901 gttatcaagg agggcattcg tagccgtctc tttgattcca ttgaagctgc ccttcgtatc
    30961 gcagaaggtt atgtgattat cgacaccatg gacgactctg agttgttgtt ctctgagcat
    31021 tatgcctgtc cagtttgtgg atttactgtc ccagagttag aacctcgtct cttctctttc
    31081 aatgctccct ttggctcttg tagtgagtgt gacggcttgg ggattaagct ggaggtggat
    31141 actgatttgg tagtgccaga tgccagcaaa accttacatg agggagcgct ggcaccttgg
    31201 aatcctatct catccaacta ctatccaaac atgttagagc aggccatgaa agtctttgga
    31261 gtggctatgg ataagccttt tgaggacctg tcagaagaag ataagaactt gattctctac
    31321 ggctcagatg gtaaggaatt ccatttccat tatgaaaatg aatttggtgg tgtgcgcgat
    31381 atcgacattc cgtttgaggg agttatcaat aatatcaaac gtcgttacca tgaaaccaat
    31441 agcgattaca ctcgcactca gatgcgtctc tacatgaatg agctgacctg cggaacctgt
    31501 caaggctatc gtctcaatga ccaggccttg tctgtccgtg tgggcggcca gcaagggcca
    31561 catattggag aaatctcaga cctgtctatc gctgaccact tggacttggt gagccagtta
    31621 actttgtctg aaaatgaagc catcattgct cgtcccattc tcaaggaaat caaggatcgt
    31681 ttgaccttcc ttaataatgt gggtcttaac tatctaaccc tgtcacgttc agcaggaacc
    31741 ctttcaggtg gggaaagtca gcgcattcgt ttggcaaccc agattggttc caacctatca
    31801 ggtgtcctct atatcctaga cgagccgtca atcggtcttc accagaggga caatgaccgc
    31861 ctgattgcca gtctgaaaaa gatgcgtgac ttgggcaata ctcttatcgt ggtggaacac
    31921 gacgaagata ccatgcgtga agctgattat ctgattgacg ttggtcctgg tgccggtgtt
    31981 tttggtggtg agattgttgc agcaggtaca cctaagcaag tggctcgcaa cagcaagtct
    32041 atcacaggtc agtacttgtc aggcaaacgt gtcatcccag taccagaaga gcgccgtgtc
    32101 ggaaatggtc gttttattga agtgacagga gcgcgtgaga acaacttgca aaatgtcacg
    32161 gctcgctttc cactaggaaa atttatcgca gtgacaggtg tgtcgggttc agggaaatcg
    32221 actctaatca acagcattct caaaaaagcc attgcccaga agctcaaccg taattcagac
    32281 aaacctggta agtttaagac tattacaggg attgagcatg tcgaccgttt gattgatatt
    32341 gaccagagcc ctatcggacg gacgccgagg tctaacccag ctacctatac aggagttttt
    32401 gacgatatac gtgacctttt tgctcagaca aatgaagcca agattcgagg ctacaagaag
    32461 gggcgcttca gtttcaacgt caagggaggt cgctgtgaag cttgctcagg tgacgggatt
    32521 atcaagattg agatgcactt cttgccagat gtttatgtgg cttgtgaagt ttgccacgga
    32581 acccgctata acagtgaaac cctagaagtt cactacaagg aaaagaatat ttcgcaggtc
    32641 ttggatatga cggtcaacga tgcggtagaa tttttccaac acattccgaa aattcaacgc
    32701 aaactccaga ccatcaagga tgtaggtcta ggctatgtga ccttgggaca gccagctacc
    32761 accctttctg ggggagaagc ccaacgtatg aagttagcta gcgaactcca caaacgctcg
    32821 acaggaaaat ctttctacat tctggatgag ccgacgacag ggcttcatac agaggacatt
    32881 gctcgcctgc tcaaggtttt agctcgcttt gtagacgccg gcaatacagt cctcgtcatc
    32941 gagcacaatc tggatgttat caagacggca gaccatatca ttgacttggg acctgagggc
    33001 ggtgtcggtg gtggaaccat catcgtaaca ggaactccag aagaagtagc tgccaacgaa
    33061 gccagctata caggacacta tttgaaagga aagttacatc atgaataaac gcgtacaagc
    33121 atttctagct aaaatgcaag aaaaagaact agatggtatc atcatcaaca atcttaaaaa
    33181 cgtctattat ttgactggtt tttggggctc aaacggaaca gtctttatca gccgtgaccg
    33241 tcaggtctta gtgacagact ctcgctatat catcgcagct aagcaagaaa ccagtggttt
    33301 tgagattgtg gctgatcgtg atgaattggc tgtcattgca ggaattgtta aggacatgaa
    33361 cttgactcgt atcggttttg aagatgagat ttcagtgtct tattaccacc gtatgcaggc
    33421 agcttttgca ggtttgaact tgcttccaca aactcagttt gtggaaggtc ttcgtatgat
    33481 taaggatgag gcagagattg cagcgattcg caaggcttgt tctatctcag accaagcttt
    33541 ccgcgatgcg cttgacttta tcaaaccagg aaaaactgaa attgagattg ccaacttcct
    33601 tgatttccgc atgcgtgagt tgggagcatc tggcttatct tttgatacga tcctagctag
    33661 cggtatcaat tcttctaaac cccatgccca tccaatgcac aaaccagtgg agttgggaga
    33721 agccattacc atggacttcg gctgtctcta tgaccactat gtcagtgata tgacccggac
    33781 tatctatcta gggcatgtta gcgatgagca ggcagagatt tacaatacgg ttctaaaagc
    33841 taaccaagcc ttgattgacc aagctaaggc aggcttaggt ttccgtgact ttgacaaaat
    33901 ccctcgtgat attatcattg aggcaggtta tggtgactac tttactcacg gcattggcca
    33961 cggtattggt ctggatatcc atgaggaacc ctactttagt cagacttcta cagaaactat
    34021 taagacaggt atggccttga ccgatgaacc aggtatctat atcgaaggca aatatggcgt
    34081 tcgtatcgag gatgatatcc tgattacaga gacaggttgt gaattattga ccctagctcc
    34141 aaaagagttg atagtcattt agttgtttgt caagaaaaaa gtacgaaaat gacgaaaaaa
    34201 gtcaaaaaaa attaaaaata ggtcgcaagt cggatgtttt ttatggtata atagactaaa
    34261 ctgatagtaa catgtagcga aaggggtagg tacatgatta aaatttatac agtctcaagt
    34321 tgtactagtt gtaaaaaagc aaaaacctgg ctcaatgccc accagttaag ttataaagaa
    34381 caaaaccttg gtaaagaagg aattacgaga gaagaattac tggatattct aaccaaaaca
    34441 gataacggaa tagccagcat cgtttcgtct aaaaatcgct atgccaaagc ccttggagtg
    34501 gatattgaag atttgagtgt caatgaagtt ctcaatctga ttatggaaac accgagaatt
    34561 ttaaagagcc caatccttgt agatgaaaaa cgcctgcaag ttggctacaa ggaagacgat
    34621 attcgtgcct tcctaccacg ctctgtccgt aatgtagaaa atgcagaagc acgtttgcgt
    34681 gcagctctat aaacatcaag gctgggagca cttcccagtc ttattctatt ttaatttcaa
    34741 aaagaaagaa gaaagaaatg aaaaaaatag ttcttgttag tctagctttc ctttttgtcc
    34801 tggttggttg cggacagaaa aaagaaactg gaccagctac aaaaacagaa aaagatacgc
    34861 ttcagtcggc attgccagtt attgaaaatg ctgagaagaa tacagttgta actaagactt
    34921 tggtcttgcc caagtcagat gatggtagcc agcagacaca aacaattact tacaaagaca
    34981 agactttttt gagtctagct atccaacaaa aacgtccagt ctctgatgag ttgaagactt
    35041 atattgacca acatggagtg gaggaaactc aaaaagctct tcttgaagcg gaggagaagg
    35101 ataagtctat cattgaagct cgtaaattgg caggtttcaa acttgaaaca aaactattga
    35161 gcgcaacgga acttcaaaca acgactagtt ttgattttca agttctggat gtcaagaagg
    35221 cttcccagtt ggaacatctg aagaatattg gtttggaaaa tcttttgaaa aatgaaccaa
    35281 gcaaatatat ttcagataga ttggcaaatg gcgcgacaga acaatagaaa atccaaataa
    35341 atagactgcc tgaattgtag aaggtaagga attatgctat aatggtacta ttctaaggaa
    35401 agagggtgtt agaatgggat ttactgaaga aacagtacgt tttaaattgg acgattccaa
    35461 taaaaaagaa attagcgaaa ctttgacaga tgtttatgct tcgttgaacg ataagggtta
    35521 caacccaatt aaccaaatcg taggttacgt attgagtgga gaccctgcct acgttcctcg
    35581 ttataataat gcacgaaatc aaatccgtaa gtatgagcgt gatgaaatcg ttgaggaatt
    35641 ggttcgctac tacctcaaag gacaaggagt cgatctataa cctatgagaa ttatgggatt
    35701 ggacgtcggt tcaaaaacgg taggggtggc gattagcgat ccgcttggtt ttacagctca
    35761 agggcttgaa atcatccaga taaatgaaga acaaggccaa tttggttttg accgcgttaa
    35821 ggaattggtt gatacttaca aggtggaacg atttgtagtg ggcttgccta aaaacatgaa
    35881 caatacaagt ggaccgcgcg tagaagctag tcaagcctac ggagcaaagc tagaagagtt
    35941 ttttggttta ccagtagact atcaggatga acgcttgaca acagtggctg ctgagcgcat
    36001 gttgattgaa caagcagata tcagtcgcaa taagcgcaag aaagtcattg ataagttagc
    36061 agctcagctg attttacaaa attatttaga tagaaaattt taatataaag gagaggctat
    36121 gtcacacgat cataaccacg accacgaaga acgtgaacta atcacactag tagatgagca
    36181 aggaaatgaa accttgtttg aaatcctttt gacaattgat ggaaaagaag aatttggtaa
    36241 aaactatgtt cttctagtac cagttaacgc agaagaagac gaagacggac aagttgaaat
    36301 ccaagcttac tcattcattg aaaatgaaga tggaacagaa ggcgaattgc aaccaatccc
    36361 agaagactca gaagacgaat ggaacatgat tgaagaagtc ttcaacagct ttatggagga
    36421 gtgatacagt ctgggagact gtatcagccc aactccagaa atggggaaga gttggaacgt
    36481 ccagtgaaca tttttacccc aactcttaga aattgagaag agttggaaca tctagtggat
    36541 gtttttagcc catattcaca ttcatagtag ctagttatta gctaaccagg taagaggtcg
    36601 ggacgaaaat cctggccttg tttttattag taaccatact ttaatgcgat aattgattgg
    36661 acttttgtca aggagatgtg tatgtttgaa gtagaagaat ggctccatag tcggattggt
    36721 ttgaattttc gatcaggttt gggccgaatg cagcaagcgg tggatttgtt aggaaatcct
    36781 gagcagtctt accctattat ccacgtaaca gggactaatg ggaaaggatc taccattgct
    36841 tttatgaggg aattatttat ggggcatggc aaaaaagttg cgacctttac ctcccctcat
    36901 atcgtctcta tcaatgaccg aatctgcatt aatgggcaac ctatagcaga cgcagacttt
    36961 atccgtttga ctgatcaggt caaggagatg gagaaaacgc ttctgcaaac tcctgcccag
    37021 ttgtcctttt ttgaattgct gaccttagtt gcttttcttt attttaggga gcaagaggtg
    37081 gatttggttt tattagaagt gggaattggt ggcttacttg acacgaccaa tgtggtaact
    37141 ggagagcttg ctgtcatcac ctccattggg cttgaccatc aagaaacctt gggtgatagt
    37201 ctagaagcaa ttgcagagca gaaagctggt attttcaagg ctggtaaaaa ggcagtgatt
    37261 gcgaaattgc ctccagaagc tagacttgtc tgtcagaaaa aagccgaatc tttagctgtt
    37321 gacctttatc aggcaggtca agatttttca gtgctgaatg gtgatttttc aagctcttta
    37381 ctaaatcttt cacagctgaa cataggctta gaaggagcct atcagcagga gaatgcggcc
    37441 ttggcgttgc aaacttttct tctttttatg ggagaaagaa aggaagctat tgatgaacag
    37501 gctgtaagaa aggccttgga acagacccat tgggctggtc gcttggagcg tattcgccca
    37561 cagatttact tggatggtgc tcataacctc cctgccttga ctcgcttggt tgagtttatc
    37621 aaagaaaaag agcaggaagg ctatcgacct caaatcctct ttggatcctt gaaacgtaag
    37681 gattatcaag ggatgttggg ttatctgact gaaaatttac ctcaagtgga actcaaaatg
    37741 accggctttg actatcaagg gtctttggac gagacagatg taacaggtta ccatgtaatt
    37801 ccctcttacc gagaatttat cagcagtttc gaagaaaggg cagacgctca agacttgctg
    37861 ttcgttacag ggtctctcta ttttatctca gaagtacggg gctacctgct ggaccgtgag
    37921 cagataaatt gacccccttt tttgaacttg ttatactagg ggaattgctg gttagagaaa
    37981 atttctctaa attggtagca gaaaggaaat tcatcatgaa attaaaaaga ttcacacttt
    38041 ctcttgcttc tctagcaagt tttagtctct tagtagcttg ttcacaaaga gctcaacagg
    38101 ttcaacagcc tgttgctcag caggtccaac agcctgctca acagaatacc aatactgcaa
    38161 atgcaggagg taaccaaaat caagcggctc cagtacaaaa ccaacctgtt gctcaaccga
    38221 ccgatattga tgggacttat actggtcagg atgacggaga ccgtatcact ttagtggtaa
    38281 ctggaacgac tggtacatgg actgagctcg aatctgacgg ggatcagaaa gtcaaacagg
    38341 ttacatttga ttcagcaaat caacgcatga ttattggcga tgatgtcaaa atttacactg
    38401 taaacggtaa tcaaatcgtc gtagatgata tggatagaga cccatcggac caaatcgttt
    38461 taactaaata agactatgca agtagaaagg gctggatttt tcagcctttt tacttttacc
    38521 tagaaaataa tcataaatac ttgccttcta ccgaatacct gcgttatact agtatcaatt
    38581 acttgttttt agcattcaaa ctatcaagga ggggatatga aatataggaa atttcaatta
    38641 ttgatgtcca agtatggctt tagtctttcg attatgctac ttgaactttg tcttgttttt
    38701 ggtctctttc tttatttagg acgcatggct cccattttat ggattactgt cctcattcta
    38761 ctgagtatca tcacaatcat ttcgatagtc aaccgtaata cgactcctga gaataaggta
    38821 acctggttgt tagtagcctt tgtgccagta tttggtccct tgctctatct gatgtttggt
    38881 gaaaggcgat tgtccaaaaa agaaatcaaa caactgaaga agctaggctc tatgcatttc
    38941 caagaagcaa atagccagct actaaaagag aaattaaaag aaagtgacaa ggcagcttat
    39001 ggagtcatca agtccttatt gagtatggat accaatgctg acatctatga tcaaactgcc
    39061 tctacatttt ttcctaacgg agaagctatg tggaaaaaga tggtagaaga tcttaaaaag
    39121 gctgagaaat ttattttctt ggaatattac attatagaag aaggtttgat gtggaatcgc
    39181 atactagata tactagagca aaaggtagct cagggtgtag aggttaagat gctctatgat
    39241 gatatcggct gtatggctac tttaacagga gattatgcac atcgacttcg tcagctgggc
    39301 atcgaggccc ataaattcaa taaagttatt cctcgtttga cagtggctta taataacaga
    39361 gatcatagaa aaatattgat tgttgatggt cagatagcct atactggtgg ggtcaatctg
    39421 gcagatgagt acattaacca cgtcgagaga tttggttatt ggaaggatag tggaattcgc
    39481 ttagacggac tagcagtaaa agccctgaca cgcttatttt tgaccacttg gtacattaat
    39541 cgaggagaaa ttagtgattt tgatcaatat catttagaaa atcattctat cccgagtgac
    39601 ggtttaacca ttccatacgg aagtggaccc aagccaattt ttcgagcgca ggtagggaaa
    39661 aaagtttatc agagtttaat caatcaagca acagaatcgg tctatattac gacaccttat
    39721 ttgattatag attatgattt aacagagaca atcaaaaatg cagctatgag aggggtcgat
    39781 gttcgaatta tcacccctta cataccagat aagaagttca ttcagttagt cacgagagga
    39841 gcttatcccg accttctttc tgctggtgtt cggatttatg agtatagtcc aggttttatt
    39901 catagtaagc agatgttggt agacgaagat tttgcggtgg tggggacaat caatctcgac
    39961 taccggagct tggtacacca ttatgaaaat gcagtcttac tctataaaac tccttctata
    40021 agggaaatcg cccgagattt tcgaaatata tttgcagatt ctcaggaagt ctatcctcat
    40081 tctatcaaaa cgagctggta tcaaaagctt gtaaaagaaa tcgcccagct attcgcccct
    40141 atcttataag aaatctagaa cttaggatac aaaccagacc tatttttctt gtcttttact
    40201 aatagaaaga aagaggaggg gaattcatgc taactaagga agaagtgaat gatatgattg
    40261 agttcaaaca gacacattta taccgctttc gggaaattga aacctttgtg aaactatgca
    40321 aaaaatcact caaacagcca tcgcaagctg acaatcctag gactttttga tatactaaga
    40381 cagataaatt gaacaggaga aacgatatga gtgtgtatgg tagagtagaa gaagttcata
    40441 aggagaatcg tgaacctcta gaataccaaa tcgaacaaga atcgcatcat cgtgaatcaa
    40501 gtcgtcttcc tttggtgaaa attttactat ggagtacgct tgtaacgggg ataactctag
    40561 gagtaccgct attactcgac ttaatgagtg cacaagaagt gcaggatttt tatgcaggtt
    40621 gggggcttca tcagacaggg aagatttaca gcgactatta tggaagtcaa ggtttgcttt
    40681 attatttgct gacttacgtg agtcagggcg gatttttctt tgccatcttt gagtggttag
    40741 ccttggtagc aggaggattt ttccttttta gatcagcgga caccttgaca gagcaaggag
    40801 accaagctgg acatctggtg actatttttt acatgctagt tacaggtctt gcttttggtg
    40861 gaggctatgc gactctttta gcgcttcctt tcttattcgc agcctttagt ttagttgcgg
    40921 cttacctaag caatccaagc catgataagg gatttgtacg gattgggcta gctttggcag
    40981 gcggattttt ctttgctccc ttatcatcgc tcctgtttat tgctgtagtg agtttaggct
    41041 tgttggtctt taaccttggg catagacgct ttgcgcatgg gttttatcag tttcttgcag
    41101 tggctttagg tttttcactt gtcttttatc caactgccta ctatagtgct gcaacaggaa
    41161 gttttgggga tgcgattagt ggtattcgtt atcctattga cagtattcgc tttgatttta
    41221 cttctaaaat tttagagaat atgttttttt atggcttgtt gtcccttggt ttgggatttg
    41281 tggtttgtat ctttttaggt ctctttcaat ccaaaccctt taaactatac gtcatttcgg
    41341 ttcctgcaag tcttgtggta attttaggtt tgattttact tttcttttca caagagcctc
    41401 tgcacgcttc ttatttgatg gtcgtcttcc ctgttttcct acttttattg gtaaccaata
    41461 ttaagagtca acagaggggg cgtagtgcta gaagaagccg aagagaaacg ccagttagcc
    41521 tatggagtcg tttcttcaaa ggaaatctat atctgctagt ttttgggttt gtctatcttt
    41581 tgtctgttcc ttttttgatg aagtttgtcc tttatccagt accttatcaa gaacgtaatc
    41641 gtcttgctga tttggtaaaa gaggagacaa atacggaaga tgctatctat gcatgggatg
    41701 atactgcgac tctttatcgt aagagtgagc gcttgtcgcc atcggcgatt ttgtccccgt
    41761 tgcactatac agcaactgag gaaaatcgta ataagttact taatgacttg aaagaaaaac
    41821 aacctaaggt gattgtggta aatgacaagg tggtagtctg gtctgaagtg gaaacacttt
    41881 taaaagaaaa ttaccaacaa gtaaagactg attactcaga gtttaaagtc tataaaatta
    41941 aataaccaaa tcaatatctt gtgtattttt aaaaatttta ggatttttaa cacaagatat
    42001 tgatttttct ttttagagtg gtataatact ttttagaaag aacattttag aaaagagcat
    42061 gcgtatgatt gcactagaag aaaaaattac aattttgcca actctcttcg tcgagaaacg
    42121 agatgggaga cgtgttgtat ttgatgtgga caagattgac aaggctctcc acaaggcggc
    42181 tgacaaggtt atggatgtga cacccctggt tgaaaaacgc ctcaatgatc tgactgagcg
    42241 aattattaca gaaattcata gtcgctttcc acagggaatt aagatttacg aaattcaaaa
    42301 tatcgtagaa catgaactcc ttgaagccaa agaatatgcg ctggctgagg agtatattac
    42361 ttatcggaca cagagggatt ttgagcgctc aaaagcgacg gatatcaact ttagtattca
    42421 taaacttctc aacaaagacc agacagttgt caatgaaaat gctaataaag acagtgatgt
    42481 ctttaacact cagcgtgatt tgacagcagg gattgttggg aaatcaatcg gactgcaaat
    42541 gcttcctaag cacgtagcca atgcccacca aaagggggat atccactatc acgatttgga
    42601 ctacagtccc tataccccta tgaccaactg ctgtttgatt gattttaagg gtatgttgga
    42661 aaatggtttt aagattggaa atgcagaggt agagagtccc aagtctatcc agactgcgac
    42721 agcacagatt tctcaaatca ttgccaacgt tgcttctagc cagtacggtg gctgttcagc
    42781 tgaccgtatc gatgaaattt tggcgcctta tgcagagaag aattatcaaa aacatctcaa
    42841 agatgcagaa gagtgggtat tgcctgaaaa acgggaagat tacgcttgga agaaaacgca
    42901 aaaggacatc tacgatgcca tgcaatctct tgagtatgaa atcaatactc tcttcacttc
    42961 aaatggacaa acacctttta cttcgttagg ttttggtctg ggaaccagtc gttttgaacg
    43021 agaaattcaa aaagctattt taaacattcg catcaagggt cttggttcag aacaccgtac
    43081 ggctatcttt cctaaactta ttttcactct gaaaagaggc cttaatttag aggaaggaac
    43141 tcccaactac gacatcaagc agttggctct tgagtgtgca accaaacgga tgtatccaga
    43201 tgttttatct tacgataaga ttgttgactt gacaggatcc ttcaaggtgc ctatgggttg
    43261 ccgttctttc ctccaaggat ggaaggatga aaatggtgta gaagtcaatt caggtcgcat
    43321 gaatctgggt gttgtgacgg ttaatctgcc tcgtattgct cttgagtctg aaggtgatat
    43381 gaataagttc tgggaaatct tcaacgagcg aatgaatatc gcagaagatg ctcttgttta
    43441 ccgtgtcgaa cgcactaaag aggcgacacc agcaaatgct cctattcttt atcagtacgg
    43501 tgcttttggc catcgtctag gtaaagaaga aagtgttgac cagctcttta agaatcgtcg
    43561 tgctaccgtt tcgctgggct atatcggctt gtatgaagta gcgacagttt tctttggtaa
    43621 cagctgggaa agtaatccag atgctaagga attcacgcta gacatcattc acgatatgaa
    43681 acgccgtgta gaagagtggt cagaccaata tggctaccat ttctctatct actcaacacc
    43741 atccgaaagt ctgacagacc gtttctgtcg actagatata gacaagtttg gctctattcc
    43801 tgatatcaca gacaaggaat actacaccaa ctctttccac tacgatgttc gtaaaaatcc
    43861 aacaccgttt gaaaaattgg actttgagaa agtctatcca gaagcaggtg cgtcaggtgg
    43921 tttcatccat tattgtgagt atccagtcct tcagcaaaat ccaaaggcct tggaagctgt
    43981 ctgggattat gcttatgacc gtgtaggcta tctaggcacc aatactccga ttgaccgttg
    44041 ctacaagtgt gactttgaag gggattttga accaactgag agagggtttg cttgtccaaa
    44101 ctgtggcaat agcgacccta aaacagtaga tgtggtgaag cgaacttgtg gctacctagg
    44161 taatcctcaa gcaagaccga tggtcaacgg ccgtcacaag gaaatcgctg cgcgtgtcaa
    44221 acatatgaat ggttcaacga ttaaaatagc tgggcatcaa gtaacaaatt agaaagaaat
    44281 gaaatgggaa aatatcaact agacgataag gggcgcgcac aagtgacccg ttatcacgag
    44341 aaacactcta aaggtggagc tggtaagaaa gaacgcttgc ttagcttcag agaacaattt
    44401 ttaaacaaga acaagaaaaa ataaaagtga gagccagctc tcgcttttct catagtggga
    44461 ggtaaggatg gaattacgca gaccaagatt agcggataag aaagctgttt tagatatgat
    44521 gacagagttt gaaaaatttc agtcgcctca cgacggcggt ttctgggata cagagaactt
    44581 tgtgtatgaa gactggttag aaagcaatca ggaacaggaa atggggatta atctgcctga
    44641 aggatgggtt cctgcaattc agttagtggc tttttctgag aaaggtcaag cagttggatt
    44701 tcttaatctc cggttgcgcc tcagtaactt tctactagaa gaaggtggcc acattggcta
    44761 ctccattcgt ccatctgaaa gaggcaaggg ttatgcaaaa gagactctcc gtcagggctt
    44821 gcaagttgct aaggaaaaga acatcaagaa agctctggtg acctgtagcg tgaataatcc
    44881 tgctagcaga gcagtcattc tagcaaatgg tggaatattt gaggatgctc gcaatggagt
    44941 cgagcgttat tggatagagg tagcgaatga ataatccaaa accacaagaa tggaaaagcg
    45001 aggaacttag tcaaggtcgt atcattgact acaaggcctt taactttgtg gacggcgaag
    45061 gcgtgcgtaa ctctctctat gtatcaggct gtatgtttca ctgcgaggga tgttataatg
    45121 ttgcaacttg gtcttttaat gctggcattc cctatacagc agaattagaa gagcagatca
    45181 tggcagatct tgctcagccc tatgttcaag gtttgacttt gttgggaggg gagccttttc
    45241 ttaatactgg gattctcttg ccacttgtta agcggattcg gaaggaattg ccagacaagg
    45301 acatctggtc ctggaccggc tacacttggg aagaaatgat gttggaaact ccagataaac
    45361 tggaattctt gtcactgatt gacattcttg tcgatggaag atatgatcga actaagagaa
    45421 atcttatgct ccagtttcga ggttcatcta accaacgaat tatcgatgtg caaaaatcgc
    45481 tcaaaagtgg gcaagtagtg atttgggaca agctcaatga cggaaaagaa agctatgaac
    45541 aggtgaagag agaatgaaga aaaaatgaag aaaaaagact tagtagacca actagtctca
    45601 gagatcgaga cggggaaagt caggacactg ggaatatacg gtcatggagc ttcaggtaaa
    45661 tcaacctttg cacaggaatt gtaccaagct ttagattcta ctacagtaaa tttgctagaa
    45721 acagatccct atatcacctc cggacgctat ttggtagtac ccaaggacgc gccgaatcaa
    45781 aaggtgacag ccagtctgcc agtggcgcat gaactggaga gtttgcagag agatatcctt
    45841 gccttgcagg cgggtatgga tgtcttgaca attgaagaac cttggaaggc tagtgaggtc
    45901 ttgtctggag ccaaaccaat tttgattgtc gaagggatgt ctgttggttt tctacccaag
    45961 gaactctttg aaaaaaccat ctgtttctac acggatgagg agaccgaatt aaagcgacgc
    46021 cttgctagag atacgactgt gagaaatcgc tatgcatcct ttatattggc tagccatcag
    46081 atgagacggg agcagtatct gcgatactat aaagaaactg agtctaaggc ggatatctta
    46141 gtggaccaat cagaagataa atttgatgtc aagaggactt aacttatgta tagaagaaat
    46201 atagaagaaa atccctaatt ttagaaagaa aaagtaggaa aacagtttca tcccaaaaaa
    46261 acgaaaaaac ctaacaaatc ccttgcaatc gcaggggatt tgtgttattc tattatggtg
    46321 ctgtaaatta cagccttagt tttgatgcaa gaggttgcga cacgctcggt tgcattgcca
    46381 cgcaacaccg cgtcggtttt cttgtggagc tagcctatta tcttaaatag acgaaaagga
    46441 gaaaaagatg gcaaacaaaa aaatccgtat ccgtttgaaa gcttacgaac accgtacgct
    46501 tgacacagcg gctgcaaaaa tcgtagaatc agctactcgt acaggtgcac aagttgcggg
    46561 tccaatccca cttccaactg aacgtagcct ctacacaatc attcgtgcga ctcacaaata
    46621 caaagactct cgcgaacaat ttgaaatgcg tacacacaaa cgtttgatcg atatcgttaa
    46681 cccaactcaa aaaacagttg atgccttgat gaaattggat cttccaagtg gtgtaaacgt
    46741 agaaatcaaa ctttaatcta aaatataaaa gagcagaggc tggtgtttca atctaattga
    46801 acacgggcta aactcggtgt gaaaaagata aacttcctcg cgtctgctag acactgtgtc
    46861 agtttcctat tttcactttg agtttgacgc cctttgtatc ttagacttga gcataaaaaa
    46921 cgctcgttaa aaactttttg aataaaaaat atagaaaagg aactattttc tcatgacaaa
    46981 aggaatctta gggaaaaaag tgggaatgac tcaaatcttc actgaagctg gcgaattgat
    47041 ccctgtaaca gttattgaag caactccaaa cgttgttctt caagttaaaa ctgttgaaac
    47101 agacggatac aacgctatcc aagttggttt cgatgacaaa cgcgaagtat tgagcaacaa
    47161 acctgctaaa ggacatgtag cgaaagctaa cacggctcct aagcgcttca ttcgtgaatt
    47221 caaaaacgtt gaaggcttgg aagttggtgc tgaaattaca gttgaaacat tcgcagctgg
    47281 agacgttgtt gacgtaactg gtacttctaa aggtaaaggt ttccaaggtg ttatcaaacg
    47341 ccacggacaa tcacgtggac caatggctca cggttctcgt taccaccgtc gtccaggttc
    47401 tatgggacct gttgcaccta accgcgtatt caaaggtaaa aaccttgcag ggcgtatggg
    47461 tggcgaccgc gtaacaattc aaaaccttga agttgtacaa gttgttccag aaaagaacgt
    47521 tatccttatc aaaggtaacg taccaggtgc taagaaatct cttatcacta tcaaatcagc
    47581 agttaaagct ggtaaataat aaagaaaggg gaaatcagtc acaatggcaa acgtaacatt
    47641 atttgaccaa actggtaaag aagctggcca agttgttctt agcgatgcag tatttggtat
    47701 cgaaccaaat gaatcagttg tgtttgatgt aatcatcagc caacgcgcaa gccttcgtca
    47761 aggaacacac gctgttaaaa accgctctgc agtatcaggt ggtggacgca aaccatggcg
    47821 tcaaaaagga actggacgtg ctcgtcaagg ttctatccgc tcaccacaat ggcgtggtgg
    47881 tggtgttgtc ttcggaccaa ctccacgttc atacggctac aaacttccac aaaaagttcg
    47941 tcgcctagct cttaaatcag tttactctga aaaagttgct gaaaacaaat tcgtagctgt
    48001 agacgctctt tcatttacag ctccaaaaac tgctgaattt gcaaaagttc ttgcagcatt
    48061 gagcatcgat tctaaagttc ttgttatcct tgaagaagga aatgaattcg cagctctttc
    48121 agctcgtaac cttccaaacg tgaaagttgc aactgctaca actgcaagtg ttcttgacat
    48181 cgcaaatagc gacaaacttc ttgtcacaca agcagctatc tctaaaatcg aggaggttct
    48241 tgcataatga atttgtatga tgttatcaaa aaacctgtca tcactgaaag ctcaatggct
    48301 caacttgaag cagggaaata tgtatttgaa gttgacactc gtgcacacaa acttttgatc
    48361 aagcaagctg ttgaagctgc tttcgaaggt gttaaagttg ccaatgttaa cacaatcaac
    48421 gtaaaaccaa aagctaaacg tgttggacgt tacactggtt ttactaacaa aactaaaaaa
    48481 gctatcatca cacttacagc tgattctaaa gcaatcgagt tgtttgctgc tgaagctgaa
    48541 taatctaagg aggaaatatc gtgggaattc gtgtttataa accaacaaca aacggtcgcc
    48601 gtaatatgac ttctttggat ttcgctgaaa tcacaacaag cactcctgaa aaatcattgc
    48661 ttgttgcatt gaagagcaag gctggtcgta acaacaacgg tcgtatcaca gttcgtcacc
    48721 aaggtggtgg acacaaacgt ttttaccgtt tggttgactt caaacgtaat aaagacaacg
    48781 ttgaagcagt tgttaaaaca atcgagtacg atccaaaccg ttctgcaaac atcgctcttg
    48841 tacactacac tgacggtgtg aaagcataca tcatcgctcc aaaaggtctt gaagtaggtc
    48901 aacgtatcgt ttcaggtcca gaagcagata tcaaagtcgg aaacgctctt ccacttgcta
    48961 acatcccagt tggtactttg attcacaaca tcgagttgaa accaggtcgt ggtggtgaat
    49021 tggtacgtgc tgctggtgca tctgctcaag tattgggttc tgaaggtaaa tatgttcttg
    49081 ttcgtcttca atcaggtgaa gttcgtatga ttcttggaac ttgccgtgct acagttggtg
    49141 ttgtcggaaa cgaacaacat ggacttgtaa accttggtaa agcaggacgt agccgttgga
    49201 aaggtatccg cccaacagtt cgtggttctg taatgaaccc taacgatcac ccacacggtg
    49261 gtggtgaagg taaagcacca gttggtcgta aagcaccatc tactccatgg ggcaaacctg
    49321 ctcttggtct taaaactcgt aacaagaaag cgaaatctga caaacttatc gttcgtcgtc
    49381 gcaacgagaa ataatattaa actagtcgct taagcaacta gtaaatccgc cagctcggta
    49441 gcgctccata ggagtgcaag ccgctgtggt acaacattta aaggagaaaa tataaaaatg
    49501 ggacgcagtc ttaaaaaagg acctttcgtc gatgagcatt tgatgaaaaa agttgaagct
    49561 caagctaacg acgaaaagaa aaaagttatt aaaacttggt cacgtcgttc aacgatcttc
    49621 ccaagtttca ttggttacac tattgcagtt tatgacggac gtaaacacgt acctgtttac
    49681 atccaagaag acatggtagg ccacaaactt ggtgaatttg caccaactcg tacttacaaa
    49741 ggtcacgctg cagacgacaa gaaaacacgt agaaaataag gagaacataa atggcagaaa
    49801 ttacttcagc taaagcaatg gctcgtacag tacgtgtttc acctcgtaaa tcacgtcttg
    49861 ttcttgataa catccgtggt aaaagcgtag ccgatgcaat cgcaatcttg acattcactc
    49921 caaacaaagc tgctgaaatc atcttgaaag ttttgaactc agctgtagct aacgctgaaa
    49981 acaactttgg tttggataaa gctaatttgg tagtatctga agcattcgca aacgaaggac
    50041 caactatgaa acgtttccgt ccacgtgcga aaggttcagc ttcaccaatc aacaaacgta
    50101 cagctcacat cactgtagct gttgcagaaa aataaggagg taaaatcgtg ggtcaaaaag
    50161 tacatccaat tggtatgcgt gtcggcatca tccgtgattg ggatgccaaa tggtatgctg
    50221 aaaaagaata cgcggattac cttcatgaag atcttgcaat ccgtaaattc gttcaaaaag
    50281 aacttgctga cgcagcagtt tcaactattg aaatcgaacg cgcagtaaac aaagttaacg
    50341 tttcacttca cactgctaaa ccaggtatgg ttatcggtaa aggtggtgct aacgttgatg
    50401 cactccgtgc aaaacttaac aaattgactg gaaaacaagt acacatcaac atcatcgaaa
    50461 tcaaacaacc tgatttggat gctcaccttg taggtgaagg aattgctcgt caattggagc
    50521 aacgtgttgc tttccgtcgt gcacaaaaac aagcaatcca acgtgcaatg cgtgctggag
    50581 ctaaaggaat caaaactcaa gtatcaggtc gtttgaacgg tgcagatatc gcccgtgctg
    50641 aaggatactc tgaaggaact gttccgcttc acacacttcg tgcagatatc gattacgctt
    50701 gggaagaagc agatactaca tacggtaaac ttggtgttaa agtatggatc taccgtggtg
    50761 aagttcttcc agctcgtaaa aacactaaag gaggtaaata accaatgtta gtacctaaac
    50821 gtgttaaaca ccgtcgtgag ttccgtggaa aaatgcgcgg tgaagcaaaa ggtggaaaag
    50881 aagtagcatt cggtgaatac ggtcttcaag ctacaactag ccactggatc actaaccgcc
    50941 aaatcgaagc tgctcgtatc gccatgactc gttacatgaa acgtggtggt aaagtttgga
    51001 ttaaaatctt cccacacaaa tcatacactg ctaaagctat cggtgtgcgt atgggatctg
    51061 gtaaaggggc acctgaaggt tgggtagcac cagttaaacg tggtaaagtg atgttcgaaa
    51121 tcgctggtgt atctgaagag attgcacgtg aagcgcttcg acttgctagc cacaaattgc
    51181 cagttaaatg taaattcgta aaacgtgaag cagaataagg agaaggcatg aaacttaatg
    51241 aagtaaaaga atttgttaaa gaacttcgtg gtctttctca agaagaactc gcgaagcgcg
    51301 aaaacgaatt gaaaaaagaa ttgtttgaac ttcgtttcca agctgctact ggtcaattgg
    51361 aacaaacagc tcgcttgaaa gaagttaaaa aacaaatcgc tcgtatcaaa acagttcaat
    51421 ctgaagcgaa ataatagact agggaaggag aaatttcaat ggaacgcaat aatcgtaaag
    51481 ttcttgttgg acgtgttgta tctgacaaaa tggacaagac aatcacagtt gtagttgaaa
    51541 caaaacgtaa ccacccagtc tatggtaaac gtattaacta ctctaaaaaa tacaaagctc
    51601 atgatgaaaa caatgttgcc aaagaaggcg atatcgtacg tatcatggaa actcgcccgc
    51661 tttcagctac aaaacgtttc cgtcttgtag aagttgttga agaagcggtc atcatctaat
    51721 caaacctgaa aggagaaaac tgaaatgatt caaacagaaa ctcgtttgaa agtcgcagac
    51781 aacagcggtg ctcgcgaaat cttgactatc aaagttcttg gtggttcagg acgtaaattt
    51841 gcaaacatcg gtgatgttat cgtggcatct gtaaaacaag ctactcctgg tggtgcggtt
    51901 aaaaaaggtg acgttgttaa agcagttatc gttcgtacta aatcaggtgc tcgtcgtgct
    51961 gatggttcat acatcaaatt tgacgaaaac gcagcagtta tcatccgtga agacaaaact
    52021 cctcgcggaa cacgtatctt tggcccagtt gcacgtgaat tgcgtgaagg tggcttcatg
    52081 aagatcgtgt cacttgctcc agaagtactt taatttttag gaacaaacta gtcccctagc
    52141 ttcaagctag ggtgccctta tgggcgtaag aaaaatcaag gagaaaccta atgtttgtaa
    52201 aaaaaggcga caaagttcgc gtaatcgctg gtaaagataa gggaacagaa gctgttgtcc
    52261 ttactgccct tccaaaagta aacaaagtta tcgttgaagg tgttaacatt gttaagaaac
    52321 accaacgtcc aactaacgag cttcctcaag gtggtatcat cgagaaagaa gcagctatcc
    52381 acgtatcaaa cgttcaagtt ttggacaaaa atggtgtagc tggtcgtgtt ggttacaaat
    52441 ttgtagacgg taaaaaagtt cgctacaaca aaaaatcagg cgaagtgctt gattaatcac
    52501 gaaggaaagg agaagtataa tggcaaatcg tttaaaagaa aaatatctta atgaagtagt
    52561 tcctgctttg acagaacaat tcaactactc atcagtgatg gctgtgccta aagtagataa
    52621 gattgttttg aacatgggtg ttggtgaagc tgtatcaaac gctaaaagcc ttgaaaaagc
    52681 tgctgaagaa ttggcactta tctcaggtca aaaaccactt atcactaaag ctaaaaaatc
    52741 aatcgccggc ttccgtcttc gtgaaggtgt tgcgatcggt gcaaaagtta cccttcgtgg
    52801 tgaacgtatg tacgaattct tggataaatt ggtatcagtt tcacttccac gtgtacgtga
    52861 cttccacggt gttccaacaa aatcatttga tggacgcggg aactacacac ttggtgtgaa
    52921 agaacaatta atcttcccag aaatcaactt cgatgacgtt gacaaaactc gtggtcttga
    52981 catcgttatc gtaacaactg ctaacactga cgaagagtca cgtgcattgc ttacaggcct
    53041 tggaatgcct tttgcaaaat aatataggag gtaaatctaa tggctaaaaa atcaatggta
    53101 gctagagagg ctaaacgcca aaaaattgtt gaccgttatg ctgaaaaacg tgctgcatta
    53161 aaggcggcag gggactacga aggtttatct aaattacctc gcaacgcttc accgactcgt
    53221 ttacataatc gttgtagagt tacggggcgc ccacattcag tttaccgcaa atttggtctg
    53281 agtcgtatcg cttttcgcga acttgcgcat aaaggtcaaa ttcctggtgt aacaaaagca
    53341 tcttggtaat ttaagatatc aagggcgtca aaactccaag taaaaatagg aaacttgacg
    53401 aagaaactaa agtttctagg aaagtttatc tttttcacac agagtttagc ccgggttcaa
    53461 ttgggcttgc caatttgaac acgagctaca gctttggcaa aaaagaccaa tttgctttgg
    53521 agcattgctt ctgcattaaa ttgtctattt ttgctcgtgc tgttacgctc tttgtatcat
    53581 gtattaacta gcaagtgcaa cttgcaaact actagtaaga ggagaaaaac aaaatggtta
    53641 tgactgaccc aatcgcagac ttcctaactc gtattcgtaa tgctaaccaa gctaaacacg
    53701 aagtacttga agtacctgca tcaaacatca aaaaagggat tgctgaaatc cttaaacgcg
    53761 aaggttttgt aaaaaacgtt gaaatcatcg aagatgacaa acaaggcgtc atccgtgtat
    53821 ttcttaaata cggaccaaat ggtgaaaaag ttatcactaa cttgaaacgt gtttctaaac
    53881 caggacttcg tgtctacaaa aaacgtgaag accttccaaa agttcttaac ggacttggaa
    53941 ttgccatcct ttcaacttct gaaggtttgc ttactgataa agaagcacgc caaaagaatg
    54001 ttggtggtga ggttatcgct tacgtttggt aaaatcaaga tacaaagctc gtaaagaaca
    54061 aagcaaaatt aggaagttgg agaagtttgt ttacaaacaa gccaacttat ctattttgca
    54121 cagttcttag agcgtgttca gttcagctct tgaactaaat aagtatctga accccgtgaa
    54181 aactggccgt tttggcctga caatttaaca ggagaaaata aacatgtcac gtattggtaa
    54241 taaagttatc gtgttgcctg ctggtgttga actcgctaac aatgacaacg ttgtaactgt
    54301 aaaaggacct aaaggagaac ttactcgtga gttctcaaaa gatattgaaa tccgtgtgga
    54361 aggtactgaa gtaactcttc accgtccaaa cgattcaaaa gaaatgaaaa ctatccacgg
    54421 aactactcgt gcccttttga acaacatggt tgttggtgta tcagaaggat tcaagaaaga
    54481 acttgaaatg cgtggggttg gttaccgtgc acagcttcaa ggatctaaac ttgttttggc
    54541 tgttggtaaa tctcatccag acgaagttga agctccagaa ggaattactt ttgaacttcc
    54601 aaacccaaca acaatcgttg ttagcggaat ttcaaaagaa gtagttggtc aaacagctgc
    54661 ttacgtacgt agccttcgtt caccagaacc atataaaggt aaaggtatcc gttacgttgg
    54721 tgaattcgtt cgccgtaaag aaggtaaaac aggtaaataa tgttgagtgg ttgatcatca
    54781 accaccaacc tattttccaa ctttgtgcat agcacacgat ttaaaactaa agaggtgaaa
    54841 actgtgattt caaaaccaga taaaaacaaa ctccgccaaa aacgccaccg tcgcgttcgc
    54901 ggaaaactct ctggaactgc tgatcgccca cgtttgaacg tattccgttc taatacaggc
    54961 atctacgctc aagtgattga tgacgtagcg ggtgtaacgc tcgcaagtgc ttcaactctt
    55021 gataaagaag tttcaaaagg aactaaaact gaacaagccg ttgctgtcgg taaactcgtt
    55081 gcagaacgtg caaacgctaa aggtatttca gaagtggtgt tcgaccgcgg tggatatcta
    55141 tatcacggac gtgtgaaagc tttggctgat gcagctcgtg aaaacggatt gaaattctaa
    55201 taggaggaca ctagaaaatg gcatttaaag acaatgcagt tgaattagaa gaacgcgtag
    55261 ttgctgtcaa ccgtgttaca aaagttgtta aaggtggacg tcgtcttcgt ttcgcagctc
    55321 ttgttgttgt tggtgaccac aatggtcgcg taggatttgg tactggtaaa gctcaagaag
    55381 ttccagaagc aatccgtaaa gcagtagatg atgctaagaa aaacttgatc gaagttccta
    55441 tggttggaac aacaatccca cacgaagttc tttcagaatt cggtggagct aaagtattgt
    55501 tgaaacctgc tgtagaaggt tctggagttg ccgctggtgg tgcagttcgt gccgttgtgg
    55561 aattggcagg tgtggcagat attacatcta aatcacttgg ttctaacact ccaatcaaca
    55621 ttgttcgtgc aactgttgaa ggtttgaaac aattgaaacg cgctgaagaa attgctgccc
    55681 ttcgtggtat ttcagtttct gatttggcat aagaaagggg ataaaatggc tcaaattaaa
    55741 attactttga ctaagtctcc aatcggacgc attccatcac aacgtaaaac tgttgtagca
    55801 cttggacttg gcaaattgaa cagctctgtt attaaagaag ataacgctgc tatccgtggt
    55861 atgattacag cagtatctca cttagtaaca gttgaagaag taaactaatg aagttttagg
    55921 ggatgtgcac tgtaccatcc cctaaaacta gatatagtca tctatgatga catcgtatag
    55981 gcgagttgat gggggagaca accttttctc ccttatcggc gctagcattt tacaaaagag
    56041 gagaaaataa aaatgaaact tcatgaattg aaacctgcag aaggttctcg taaagtacgt
    56101 aaccgcgttg gtcgtggtac ttcatcaggt aacggtaaaa catctggtcg tggtcaaaaa
    56161 ggtcaaaaag ctcgtagcgg tggcggagtt cgccttggtt ttgaaggtgg acaaactcca
    56221 ttgttccgtc gtcttccaaa acgtggattc actaacatca acgctaaaga atacgcaatt
    56281 gtgaaccttg accaattgaa cgtctttgaa gatggtgctg aagtaactcc agttgttctt
    56341 atcgaagcag gaattgttaa agctgaaaag tcaggtatta aaattcttgg taacggtgag
    56401 ttgactaaga aattgactgt gaaagcagct aaattctcta aatcagctga agaagctatc
    56461 actgctaaag gtggttcagt agaagtcatc taagagaggt gacctatgtt ttttaaatta
    56521 ttaagagaag ctcttaaagt caagcaggtt cgatcaaaaa ttttatttac aatttttatc
    56581 gttttggtct ttcgtatcgg aactagcatt acagttcctg gtgtgaatgc caatagcttg
    56641 aatgctttaa gtggattatc cttcttaaac atgttgagct tggtgtcggg gaatgcccta
    56701 aaaaactttt cgatttttgc cctaggagtt agtccctata tcaccgcttc tattgttgtc
    56761 caactcttgc aaatggatat tttacccaag tttgtagagt ggggtaaaca aggggaagta
    56821 ggtcgaagaa aattgaatca agctactcgt tatattgctc tagttctcgc ttttgtgcaa
    56881 tctatcggga ttacagctgg ttttaatacc ttggctggag ctcaattgat taaaactgct
    56941 ttaactccac aagtttttct gacgattggt atcatcttaa cagctggtag tatgattgtc
    57001 acttggttgg gtgagcaaat tacagataag ggatacggaa acggtgtttc catgattatc
    57061 tttgccggga ttgtttcctc aattccagag atgattcagg gcatctatgt ggactacttt
    57121 gtgaacgtcc caagtagccg tatcacttca tctatcattt tcgtaatcat tttgattatt
    57181 actgtattgt tgattattta ctttacaact tatgttcaac aagcagaata caaaattcca
    57241 atccaatata ctaaggttgc acaaggtgct ccatctagct cttaccttcc gttaaaggta
    57301 aaccctgctg gagttatccc tgttatcttt gccagttcga ttactgcagc gcctgcggct
    57361 attcttcagt ttttgagtgc cacaggtcat gattgggctt gggtaagggt agcacaagag
    57421 atgttggcaa ctacttctcc aactggtatt gccatgtatg ctttgttgat tattctcttt
    57481 acattcttct atacgtttgt acagattaat cctgaaaaag cagcagagag cctacaaaag
    57541 agtggtgcct atatccatgg agttcgtcct ggtaaaggta cagaagaata tatgtctaaa
    57601 cttcttcgtc gtcttgcaac tgttggttcc ctcttccttg gtgtgatttc cattttaccg
    57661 attgcagcta aagatgtatt tggtctttct gatgttgttg cctttggtgg aacaagtctc
    57721 ttgatcatta tctctacagg tatcgaagga atcaagcaat tggaaggtta cctattgaaa
    57781 cgtaagtatg ttggtttcat ggacagaaca gaataaaagt atttactgaa tcagtaaata
    57841 ctgagggagt ggaggtttaa actctgacat ttgtaagagt tggatctccc ctcttctatt
    57901 ttgtttttaa ataggggtga aaagactttt tgcttctatt taaaaataaa ataaggagat
    57961 caaatcatga atcttttgat tatgggctta cctggtgcag gtaagggaac tcaagcagca
    58021 aaaatcgtag aacaattcca tgttgcacat atctcaacag gtgatatgtt ccgcgctgca
    58081 atggcaaatc aaactgaaat gggtgttctt gctaagtcat atattgacaa gggtgaattg
    58141 gttcctgacg aagttacaaa tggaatcgta aaagaacgcc tttcacaaga tgatattaaa
    58201 gaaacaggat tcttattgga tggttaccca cgtacaattg aacaagctca tgccttggac
    58261 aaaacattgg ctgaacttgg cattgaacta gaaggtatta tcaatattga agtgaaccct
    58321 gacagcctct tggaacgttt gagtggccgt atcatccacc gcgtaactgg agaaactttc
    58381 cacaaggtct ttaacccacc agttgactat aaagaagaag attactacca acgtgaagat
    58441 gataagcctg agacagtaaa acgtcgtttg gatgttaata ttgctcaagg agaaccaatc
    58501 attgctcact accgtgccaa aggtttggtt catgacatcg aaggtaatca agatatcaat
    58561 gatgtcttct cagatattga aaaagtattg acaaatttga aataaagcgt ttttcgcact
    58621 tgcaaaaatc cgctacaaat gttatactga gatagtctga cttataattg ttgtctctgt
    58681 gtctagaggc atcgaatcga aatttatgga ggtgcttttg cgtggcaaaa gacgatgtga
    58741 ttgaagttga aggcaaagta gttgatacaa tgccgaatgc aatgtttacg gttgaacttg
    58801 aaaatggaca tcagatttta gcaacagttt ctggtaaaat tcgtaaaaac tatattcgta
    58861 ttttagcggg agatcgtgtt actgtcgaaa tgagtccata tgacttgaca cgtggacgta
    58921 tcacttaccg ctttaaataa tcgaaaaact tggagggata agaaatgaaa gtaagaccat
    58981 cggtcaaacc aatttgcgaa tactgtaaag ttattcgtcg taatggtcgt gttatggtaa
    59041 tttgcccagc aaatccaaaa cacaaacaac gtcaaggata agatagaaag gagaaaacat
    59101 ggctcgtatt gctggagttg atattccaaa tgacaaacgc gtagtaatct cattgactta
    59161 tgtttatggt atcggacttg caacatctaa gaaaattttg gctgctgctg gaatctcaga
    59221 agatgttcgt gtacgtgatc ttacatcaga tcaagaagat gctatccgtc gtgaagtgga
    59281 tgcaatcaaa gttgaaggtg accttcgtcg tgaagtaaac ttgaacatca aacgtttgat
    59341 ggaaatcggt tcataccgtg gtatccgtca ccgtcgtgga cttcctgtcc gtggacaaaa
    59401 cactaaaaac aacgctcgca ctcgtaaagg taaagctgtt gcgattgctg gtaagaaaaa
    59461 ataatatagg aggtaaaagt cttggctaaa ccaacacgta aacgtcgtgt gaaaaagaat
    59521 atcgaatctg gtattgctca tattcacgct acatttaata acactattgt tatgattact
    59581 gatgtgcatg gtaatgcaat tgcttggtca tcagctggtg ctcttggttt caaaggttct
    59641 cgtaaatcta caccattcgc tgctcaaatg gcttctgaag ctgctgctaa atctgcacaa
    59701 gaacacggtc ttaaatcagt tgaagttact gtaaaaggtc caggttctgg tcgtgagtca
    59761 gctattcgtg cgcttgctgc cgctggtctt gaagtaacag caattcgtga tgtgactcca
    59821 gtgccacaca atggtgctcg tcctccaaaa cgtcgccgtg tataatcatc gcattacact
    59881 gcttttcgtt taagagggag taactaaatg atcgagtttg aaaaaccaaa tataacaaaa
    59941 attgatgaaa ataaagatta tggcaagttt gtaatcgaac cacttgaacg tggctacggt
    60001 acaactcttg gtaactctct tcgtcgtgta cttctagctt ctctaccagg agcagctgtg
    60061 acatctatca acattgatgg tgtgttacat gagtttgaca cagttccagg tgttcgtgaa
    60121 gacgtgatgc aaatcattct gaacattaaa ggaattgcag tgaaatcgta cgttgaagac
    60181 gaaaaaatca tcgaactgga tgttgaaggt cctgctgaag taacagctgg tgacattttg
    60241 acagatagcg atattgaaat tgtaaatcca gatcattatc tctttacaat cggtgaaggt
    60301 tcttctctaa aagcgactat gactgttaac agtggtcgtg gatatgtacc tgctgatgaa
    60361 aataaaaagg ataatgcacc agttggaaca cttgctgtag attctattta tacaccagtt
    60421 acaaaagtca actatcaagt ggaacctgct cgtgtaggta gcaatgatgg tttcgacaaa
    60481 ttaacccttg aaatcttgac aaatggaaca attattccag aagatgcttt agggctttca
    60541 gcacgtattt tgacagaaca tcttgatttg tttacaaatc ttactgagat tgctaagtca
    60601 actgaagtga tgaaagaagc tgatactgaa tctgacgacc gtattttaga tcgtacgatt
    60661 gaggaactgg acttgtctgt gcgttcatac aactgtttaa aacgtgccgg tatcaatact
    60721 gtgcatgatt tgacagaaaa atctgaagca gagatgatga aagtacgaaa tcttggacgc
    60781 aagagtttgg aagaagtgaa actcaaactc attgatttgg gtcttggatt aaaagataaa
    60841 taaaggagga atacatggct taccgtaaac taggacgcac tagctcacaa cgtaaagcaa
    60901 tgcttcgcga tttgacaact gaccttttga tcaacgaatc aatcgtgaca actgaagctc
    60961 gtgctaaaga aatccgtaaa actgttgaaa aaatgattac tctaggtaaa cgtggtgatt
    61021 tgcatgcacg tcgtcaagca gctgctttcg tacgtaatga aatcgcatct gaaaactatg
    61081 atgaagcaac tgataagtac acttctacta cagcacttca aaaattgttc tcagaaatcg
    61141 cacctcgtta tgctgaacgt aacggtggat acactcgtat ccttaaaact gaaccacgtc
    61201 gtggtgatgc agcgccaatg gcgatcatcg aattagtata aaatcatcaa ttttgttgag
    61261 tgttatgatg atggagtctt gtgctcttag tctagctctg gtctaccgct aggatttcgg
    61321 tcctagcggg aacactcatc ataagttggg atagtagacg cttgtttacg aaattgtttt
    61381 tttcttaaga acaacttcgt aagcaggcgt ttttgagtat tttcgttaga attatgctat
    61441 actatttgaa aagaatcctg tttaatgtta aggtttctta ttttaagaag aattggagtt
    61501 tacttatgaa agccattata actgttgttg gtaaagataa atctggaatt gttgcaggtg
    61561 tttctggtaa aattgcagaa ttaggattga atattgacga tatctctcaa actgtcttgg
    61621 atgaatattt tacgatgatg gctgttgtat ctagtgatga aaagcaagat tttacctatc
    61681 ttcgtaatga atttgaagct tttgggcaaa ctttgaatgt aaaaatcaat attcagagtg
    61741 cagcgatttt cgaagctatg tataatatct aggaggttat catggatatt agacaagtta
    61801 ctgaaaccat cgccatgatt gaggagcaaa atttcgatat cagaaccatt accatgggga
    61861 tttctctttt ggattgtatc gatccagata tcaatcgtgc tgcggagaaa atctatcaaa
    61921 aaattacgac aaaggcggct aatttagtag ctgttggtga tgaaattgcg gctgagttgg
    61981 gaattcctat cgttaataag cgtgtatcgg tgacacctat ttctctgatt ggggcagcga
    62041 cagatgcgac ggactacgtg gttctggcaa aagcgcttga taaggctgcg aaagagattg
    62101 gtgtggactt tattggtggt ttttctgcct tagtacaaaa aggttatcaa aagggagatg
    62161 agattctcat caattccatt cctcgcgctt tgactgagac ggataaggtc tgctcgtcag
    62221 tcaatatcgg ctcaaccaag tctggtatta atatgacggc tgtggcagat atgggacgaa
    62281 ttatcaagga aacggcaaat ctttcagata tgggagcggc caagttggtt gtattcgcta
    62341 atgctgttga ggacaatcca tttatggcgg gtgcctttca tggtgttggg gaagcagatg
    62401 ttatcatcaa tgtcggagtt tctggtcctg gtgtggtgaa acgtgctttg gaaaaagttc
    62461 gtggacagag ctttgatgta gtagccgaaa cagttaagaa aactgccttt aaaatcactc
    62521 gtatcggtca attggttggt caaatggcca gtgagagact gggtgtggag tttggtattg
    62581 tggacttgag tttggcacca acccctgcgg ttggagactc tgtggcacgt gtccttgagg
    62641 aaatggggct agaaacagtt ggcacgcatg gaacgacagc tgccttggcc ctcttgaacg
    62701 accaagttaa aaagggtgga gtgatggcct gtaatcaggt cggtggtcta tctggtgcct
    62761 ttatccctgt ttctgaggat gaaggaatga ttgctgcagt gcaaaatggc tctcttaatt
    62821 tagaaaaact agaagctatg acggctatct gttctgttgg attggatatg attgccatcc
    62881 cagaagatac gcctgctgaa actattgcgg ctatgattgc ggatgaagca gcaatcggtg
    62941 ttatcaacat gaaaacaaca gctgttcgta tcattcccaa aggaagagaa ggcgatatga
    63001 ttgagtttgg tggtctatta ggaactgcac ccgttatgaa ggttaatggg gcttcgtctg
    63061 tcgacttcat ctctcgcggt ggacaaatcc cagcaccaat tcatagtttt aaaaattaag
    63121 aaaataggag aaattttaag ttctatttaa gattagatgt gtatactata atcattaaat
    63181 aaagacctcc taatattatt tgaaacagat aacactgaat tagtttgaat ttgattttca
    63241 tctaatatct ttatttaatg aactcctaaa ctttttcata ataatctcct tcaaaagtcg
    63301 cctgtatggg tggcttttat tttatcattc atgatataat agaagcaaac ggaggacgga
    63361 aaatggtaaa agtacgattg tatttgatac gtcatggcaa gaccatgttt aacacgattg
    63421 gtcgcgcgca aggttggagc gatactccct taactgctga aggtgaacga gggattcaag
    63481 agttaggaat tggtttgcga gaatctgatc tacagtttga gcgcgcttat tcgagtgatt
    63541 ctggtcgtac cattcagacc atgggaatta tccttgaaga acttggcttg caggggaaaa
    63601 tcccttatcg catggacaag cgtatcagag aatggtgttt cggtagtttt gatggagcct
    63661 atgatggcga tcttttcatg ggcattattc ctcgtatctt taatgtggac cacgttcacc
    63721 aattgtctta tgctgaactg gctgagggct tggtagaggt cgatacagct ggttgggctg
    63781 aaggctggga aaaactcagt ggccgaatca aggaaggctt tgaaatgatt gcaaaagaaa
    63841 tggaagatca aggtggaggt aacgcccttg ttgtcagcca tggaatgact attggaacca
    63901 ttgtttatct gattaatggc atgcatccgc atggtctaga taatggtagc gtgacgattc
    63961 ttgaatatga ggacggtcag tttagggttg aagttgtcgg tgaccgtagt taccgagagc
    64021 taggacgtga gaagatggaa gaaggctcta tttaatcagt ctagacttgc ttgccatgag
    64081 ctagggattt gataagaata tcaagataag aaaaaacagc cgagggtact cctttcggct
    64141 gtttttgatg tggaaaacta aagtgtaatg ctattgcttt tagagatttt cataaacaag
    64201 agcaaggaac ctactgttag aacagtcagg atagttgaca aggctgcggc tacaccgtaa
    64261 tttcctctga gaacctctgt ataaatagct acagtcattg ttcttgtttt gacattgtag
    64321 aggaggatag aagtagagag ttctgaaatc agtgtgaccc aagataggat ggctccagaa
    64381 ataatatccg atagcatcat tggagttgta atcttagcaa agatattgag acgactactt
    64441 cctaagcttt cagcagcttc ttcaatactt ggtgctattt gttgtaagct agcaacagat
    64501 gagcgaatag tataaggtaa ttttctggca gatagagaca taatcaagat gaaagcagtc
    64561 cctgtaatca taagaaatcc acttccaaat agaccagaga aatgaaggca atccctagaa
    64621 cggttcctgg tacaatataa ggtaccatac tgaggctgtc aattaagttt gtaaacaaat
    64681 tccgttttct aacggctagg taggagataa atgtcgcaaa tagaacaact agaactaagg
    64741 caatcaaagg gatacgaatg gtattgaaaa tagcagatcc catacgatgg aaagctacct
    64801 tgtaactgtt tggagaataa cctttaacag ataccatacc tgatgttttt aggaaagagg
    64861 tataaattaa gtagatttga ggtaaaacag agataaagat aattccgtag actgttgcat
    64921 aaatggcagc tatttttcct tttgtagttt ttttaggctc aattggatgg agcagattca
    64981 tgctgaaact gtagcggttt gcaatgtgtt tttggataag gaaaattgcc aaggcaatga
    65041 taatcgccat aattgcaaaa gcagaatttc ctccaacctc gctaataaat tgggtataaa
    65101 tcaggacagg gaaagtccga tatccttcgc caatcaacat aggcgttcca aagtctgaga
    65161 atgctctcat aaatacaagc aaggagctgc tagtaaggtt ggaactagga gaggtaaaac
    65221 aaccgttacg atacgcttaa atccgaagga cccccatgct ttcagctgct tcaagtagag
    65281 aattgtcaat actgttcatt gttccagcaa catatagaaa taccagtggg aatagttgca
    65341 gtgtaaagac aagtacaatt cctttgaatc cataaatatc gatagctgga agatgaaggg
    65401 catttgtcaa aaatttagtg atgaccccat ttcgtcctag caagagaacc caggagtagg
    65461 ctcctacgaa aggagctgac atggaagcaa tgataatcaa tatttgtaga aatttctccc
    65521 ccttgaagtc atacatagag aagagataag ctaatagggt tcctacaact aaggaagtga
    65581 cagtagcggt aatggaaacc ttgaaactgt tgactagtgt ctcagagtag tagactttac
    65641 taaagaaagt gacaaaatta gctagtgaga attatccttc atgtataagt gcttacttga
    65701 gcacggtaat gataggataa acgagaaaga caagataggt aagaaagatg aagaaagagg
    65761 aggctgtcca aatatttagt tttttacatt ccatggttga ctccttttat caggttttgg
    65821 gaaccatctg cagaaaagat gtttaatttt tgcgtattga ttcgtagacg aatacgattg
    65881 tctttttgta gatcttcttc aaaagttgat tcttcactaa cttgaatttt tgaggcaaaa
    65941 cctgtctcaa tgaaatactc cgtatttagt ccaagataga cgctatcgct aatagttcct
    66001 tcaatatctc cagattcatc tttgataaac tcttcgggac gaatgcttac atgaatagct
    66061 tgctcctcaa cctgatcaag agctggcatt cgaagggcat agccatctga aaagacgata
    66121 taagcgccgt cgctccgttt ttcaagattg gcagggataa tatttgtgcg tccgataaag
    66181 gttgccacaa actcattagc tggtttatga tagagttctt ttggtcggcc gatttgttgg
    66241 atcaccccat ctttcataac agcaatttga tctgaaatag ccatggcttc ttcttggtcg
    66301 tgggttacat aaacagttgt aattcccact tcgtgttgga tttctcggat ggcttgacgc
    66361 atatccaagc gaagtttggc ctccagatta ctaagtggct cgtccatgag gagaacactt
    66421 ggattaaccg ctaaggcgca tgccaaggtg acacgttgtt gttgtccacc actgagttta
    66481 tcgggctttc gatccgcata ttgagcaatt tgcatgagtt caagatactt gttggtctgt
    66541 tgaatcaatt cttcttttgg aaccttcttt tgcttaagac caaaagcaac gttgtctcgg
    66601 acagtcaaat gtgggaaaat agcgtagttt tggaaaacca tcccgatatt gcgtttgctg
    66661 ggttccatat tattgatttt tgtatcatcg aagtaaaatt ctccatcttt gatactgttg
    66721 aaacctgcaa tcatacgaag aagggtcgtt ttcccacatc ctgaaggtcc aagaagggta
    66781 aagagacttc cttttggaat tgtaatgttc aaattctcaa taacagggac atcgtggtag
    66841 attttttggc gttaataatt ttgatctcac tcatagtgaa ccttttttac tgtttagatt
    66901 ggatatctgt aaagacttcg ttgtatttct taacgatatc tgatttattc ttgatgacat
    66961 aatcataatc ttcagtgagt gttttgattt tgtcaattgg tttcatgttt tcgcttgttt
    67021 tagcattttt acgaacagga cggttagtag tggttgtacc aagtgtatct tgtacttctt
    67081 gagagataat aaaatcgata aatttcttgt cattttccat atttttagct tttttaacga
    67141 tagcagcact agcaggtagg aagacggttc cttcttttgg atagactact ttaatgttag
    67201 ctccgtcatt taagagttta actgctggat cctcataaga gagaccaaca gccatttctc
    67261 catcaacgac tgctttatag acaccagatg aacttgaacc gattttatag tgtattgaaa
    67321 ctagaatagt acacctctac ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatt
    67381 aatttgtcct gttcttattt catttcacta tgccatcaat aagtgtgaaa agatctttta
    67441 cataagacca agccttatca tctttgtaac caccttgagc ttgtagcata tttgttaatt
    67501 gagcaaaggc gctagaagag tttgctgggt cagcagttgc gatttttcct tttagttcag
    67561 gtttgagaag gtcgctatat ccttcgatgt tcatgccttt agttaaatca gggttgacga
    67621 ttaaaacact accatctagt gtataaggag tagagaagcc agttgtgttt tgatattctt
    67681 tgataacatt atcattttct tttgaaatat agttttcaaa gagttctccg tgggtagcat
    67741 attgtgtata agaaccacca aagataacat cagctacagg aacttctttt tctgactcta
    67801 gttttttgaa aagttctcca gtaccagctt gaatcagttc tattttgata ccatattttt
    67861 cttcaaaggc aggaatagtt gctccaatta agccttctga gtttggtgaa taaacgacta
    67921 gcgaaccacc gtctccttta tcagatgaac tgtcatcggc agattcatta gaagaacaag
    67981 cagcataata catctatttc tttttcatga tggatatctc cgttgtgtta tttaagttta
    68041 ttttaaaaca atgtaagcgt ttttaaaacc tacaattcta
//
LOCUS       DAVKFH010000004        67333 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKFH010000004 DAVKFH010000000
VERSION     DAVKFH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.004"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(21..956)
                     /locus_tag="VW006_000238"
     CDS             complement(21..956)
                     /locus_tag="VW006_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9379157.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(953..1813)
                     /locus_tag="VW006_000239"
     CDS             complement(953..1813)
                     /locus_tag="VW006_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9379158.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRAGQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1921..4083
                     /locus_tag="VW006_000240"
     CDS             1921..4083
                     /locus_tag="VW006_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9379159.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4175..5434
                     /locus_tag="VW006_000241"
     CDS             4175..5434
                     /locus_tag="VW006_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9379160.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5448..6315
                     /locus_tag="VW006_000242"
                     /pseudo
     CDS             5448..6315
                     /locus_tag="VW006_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            6329..7162
                     /locus_tag="VW006_000243"
     CDS             6329..7162
                     /locus_tag="VW006_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9379161.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7163..7243
                     /locus_tag="VW006_000244"
                     /pseudo
     CDS             7163..7243
                     /locus_tag="VW006_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7520..8962
                     /gene="gtfA"
                     /locus_tag="VW006_000245"
     CDS             7520..8962
                     /gene="gtfA"
                     /locus_tag="VW006_000245"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9379162.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9100..10293
                     /locus_tag="VW006_000246"
     CDS             9100..10293
                     /locus_tag="VW006_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9379163.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10979..12958
                     /locus_tag="VW006_000247"
     CDS             10979..12958
                     /locus_tag="VW006_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9379164.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKTSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13025..14521
                     /locus_tag="VW006_000248"
     CDS             13025..14521
                     /locus_tag="VW006_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379165.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14521..15447
                     /locus_tag="VW006_000249"
     CDS             14521..15447
                     /locus_tag="VW006_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379166.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15456..16523
                     /locus_tag="VW006_000250"
     CDS             15456..16523
                     /locus_tag="VW006_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379167.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16534..17460
                     /locus_tag="VW006_000251"
     CDS             16534..17460
                     /locus_tag="VW006_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379168.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            18027..18887
                     /locus_tag="VW006_000252"
     CDS             18027..18887
                     /locus_tag="VW006_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9379169.1"
                     /translation="MSQFITHQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELVRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            18899..19792
                     /locus_tag="VW006_000253"
     CDS             18899..19792
                     /locus_tag="VW006_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379170.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            19805..20608
                     /locus_tag="VW006_000254"
     CDS             19805..20608
                     /locus_tag="VW006_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9379171.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20641..21351)
                     /gene="treR"
                     /locus_tag="VW006_000255"
     CDS             complement(20641..21351)
                     /gene="treR"
                     /locus_tag="VW006_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9379172.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21536..23503
                     /gene="treP"
                     /locus_tag="VW006_000256"
     CDS             21536..23503
                     /gene="treP"
                     /locus_tag="VW006_000256"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9379173.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23681..25306
                     /gene="treC"
                     /locus_tag="VW006_000257"
     CDS             23681..25306
                     /gene="treC"
                     /locus_tag="VW006_000257"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9379174.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25562..25810
                     /locus_tag="VW006_000258"
     CDS             25562..25810
                     /locus_tag="VW006_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9379175.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26017..26811
                     /gene="racE"
                     /locus_tag="VW006_000259"
     CDS             26017..26811
                     /gene="racE"
                     /locus_tag="VW006_000259"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9379176.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26808..27779
                     /locus_tag="VW006_000260"
     CDS             26808..27779
                     /locus_tag="VW006_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9379177.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27755..28276
                     /locus_tag="VW006_000261"
     CDS             27755..28276
                     /locus_tag="VW006_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9379178.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28273..28734
                     /locus_tag="VW006_000262"
     CDS             28273..28734
                     /locus_tag="VW006_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9379179.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28725..29465
                     /gene="xerD"
                     /locus_tag="VW006_000263"
     CDS             28725..29465
                     /gene="xerD"
                     /locus_tag="VW006_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9379180.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29465..30193
                     /locus_tag="VW006_000264"
     CDS             29465..30193
                     /locus_tag="VW006_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9379181.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30212..30781
                     /locus_tag="VW006_000265"
     CDS             30212..30781
                     /locus_tag="VW006_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9379182.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30768..31490
                     /locus_tag="VW006_000266"
     CDS             30768..31490
                     /locus_tag="VW006_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9379183.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31490..31732
                     /gene="yidD"
                     /locus_tag="VW006_000267"
     CDS             31490..31732
                     /gene="yidD"
                     /locus_tag="VW006_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9379184.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(31957..32922)
                     /locus_tag="VW006_000268"
     CDS             complement(31957..32922)
                     /locus_tag="VW006_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9379185.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33018..33770)
                     /locus_tag="VW006_000269"
     CDS             complement(33018..33770)
                     /locus_tag="VW006_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9379186.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(33767..34723)
                     /locus_tag="VW006_000270"
     CDS             complement(33767..34723)
                     /locus_tag="VW006_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9379187.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(34713..35672)
                     /locus_tag="VW006_000271"
     CDS             complement(34713..35672)
                     /locus_tag="VW006_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379188.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36095..36796)
                     /gene="galE"
                     /locus_tag="VW006_000272"
     CDS             complement(36095..36796)
                     /gene="galE"
                     /locus_tag="VW006_000272"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9379189.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37197..38261
                     /gene="pepA"
                     /locus_tag="VW006_000273"
     CDS             37197..38261
                     /gene="pepA"
                     /locus_tag="VW006_000273"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9379190.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38283..38579
                     /locus_tag="VW006_000274"
     CDS             38283..38579
                     /locus_tag="VW006_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9379191.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            39261..39704
                     /locus_tag="VW006_000275"
     CDS             39261..39704
                     /locus_tag="VW006_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9379192.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            39708..40472
                     /locus_tag="VW006_000276"
     CDS             39708..40472
                     /locus_tag="VW006_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9379193.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            40487..41215
                     /locus_tag="VW006_000277"
     CDS             40487..41215
                     /locus_tag="VW006_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379194.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            41208..42728
                     /locus_tag="VW006_000278"
     CDS             41208..42728
                     /locus_tag="VW006_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9379195.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            43073..43879
                     /locus_tag="VW006_000279"
     CDS             43073..43879
                     /locus_tag="VW006_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9379196.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(44045..44581)
                     /locus_tag="VW006_000280"
     CDS             complement(44045..44581)
                     /locus_tag="VW006_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9379197.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            44714..45604
                     /locus_tag="VW006_000281"
     CDS             44714..45604
                     /locus_tag="VW006_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9379198.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            45848..46201
                     /locus_tag="VW006_000282"
     CDS             45848..46201
                     /locus_tag="VW006_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9379199.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46202..47239
                     /locus_tag="VW006_000283"
     CDS             46202..47239
                     /locus_tag="VW006_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9379200.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(47286..48293)
                     /gene="galR"
                     /locus_tag="VW006_000284"
     CDS             complement(47286..48293)
                     /gene="galR"
                     /locus_tag="VW006_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9379201.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            48458..49636
                     /locus_tag="VW006_000285"
     CDS             48458..49636
                     /locus_tag="VW006_000285"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9379202.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49655..51136
                     /locus_tag="VW006_000286"
     CDS             49655..51136
                     /locus_tag="VW006_000286"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9379203.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            51206..51610
                     /locus_tag="VW006_000287"
     CDS             51206..51610
                     /locus_tag="VW006_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9379204.1"
                     /translation="MKDFHFDAISAFESYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            51791..52555
                     /locus_tag="VW006_000288"
     CDS             51791..52555
                     /locus_tag="VW006_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEU9379205.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            52552..53013
                     /locus_tag="VW006_000289"
     CDS             52552..53013
                     /locus_tag="VW006_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU9379206.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(53064..54326)
                     /locus_tag="VW006_000290"
     CDS             complement(53064..54326)
                     /locus_tag="VW006_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9379207.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGNGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(54326..54907)
                     /locus_tag="VW006_000291"
     CDS             complement(54326..54907)
                     /locus_tag="VW006_000291"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9379208.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(55307..55402)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(55785..56024)
                     /locus_tag="VW006_000292"
     CDS             complement(55785..56024)
                     /locus_tag="VW006_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9379209.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(56069..56167)
                     /locus_tag="VW006_000293"
     CDS             complement(56069..56167)
                     /locus_tag="VW006_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9379210.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            complement(56179..57006)
                     /gene="xth"
                     /locus_tag="VW006_000294"
     CDS             complement(56179..57006)
                     /gene="xth"
                     /locus_tag="VW006_000294"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9379211.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            57140..57596
                     /locus_tag="VW006_000295"
                     /pseudo
     CDS             57140..57596
                     /locus_tag="VW006_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            57593..58123
                     /locus_tag="VW006_000296"
     CDS             57593..58123
                     /locus_tag="VW006_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9379212.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            58251..59996
                     /locus_tag="VW006_000297"
     CDS             58251..59996
                     /locus_tag="VW006_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379213.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            59986..61737
                     /locus_tag="VW006_000298"
     CDS             59986..61737
                     /locus_tag="VW006_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379214.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            61910..62602
                     /locus_tag="VW006_000299"
     CDS             61910..62602
                     /locus_tag="VW006_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9379215.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            62645..63871
                     /locus_tag="VW006_000300"
     CDS             62645..63871
                     /locus_tag="VW006_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9379216.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            65002..67161
                     /gene="pfbA"
                     /locus_tag="VW006_000301"
     CDS             65002..67161
                     /gene="pfbA"
                     /locus_tag="VW006_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9379217.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            67145..>67333
                     /locus_tag="VW006_000302"
     CDS             67145..>67333
                     /locus_tag="VW006_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379218.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNC"
BASE COUNT        19741 a        12410 c        15078 g        20104 t
ORIGIN      
        1 tatattataa ttgtgttatt ttacttccaa ctattgagag aaatttcgcc actatttagc
       61 cagatttctt ttccgttatc acattgaact aaaagttttc cattttctga gatgtcttta
      121 gcaagtccct tgtagtcttt ttgctgtagt gtgaaagtga cttcttttcc tagaatgaat
      181 gactgttttt tgtataggta taatagctct tctgctggtg tttcgaagaa agcacgccag
      241 atttctatga tcaattcatt ccttgttata ggagctgtag ctttaaataa gctggcagct
      301 ttttctttta attcctgagg gaagtcttta atagtgaaat tgatacctac tccaataatg
      361 atatctgtga ctaagccagt ttctacagag gtcattgctt cagtaaggat tcctccaatt
      421 ttatgattgt ttagatagat atcattgacc cattttatgt cgacatctat taaagttagg
      481 ttcttaatag ctttgtagac agctccagct acaagtagtg tgtaggatgg taatttgtca
      541 taggggagat ttggtttaag atggagtgtc atataaatac caccttgtgg tgagtagaag
      601 gaacgttgaa aacgtcctcg gcctgctgtt tgataggaag ctagatagag ggtatttgct
      661 tcatggccta aatcaattgc ttcttttgca tctagttgtg ttgattttgt ttcgggttta
      721 aagctgactt taattggaag attttcttct agaatctctg gaagaataag gtcaccattc
      781 attagtttat atcctctatt tttgatacta tcaatttcaa tgccttcttg ttctagtcgc
      841 ttgatagctt tccaaattga tgttcggctt agggatagtt tttctgcgat tttttctccg
      901 ctgatatagt cggtttcttt agataggatt tggtagacag cttggtagga tttcataatg
      961 ttgcctttct cactagttgg tattgagagt attcttttct tgtatgactt ggagtctgat
     1021 taaagtattg tttataagct ttcgaaaaat ggagtggatc tgaaaaacct accgagtatg
     1081 caattacctt gatggactct tgggtatttt cgagaagttg tccagctcgg tgcattcgaa
     1141 cgtagagtag gtattctttg ggtgataagg tattaaattc tttgaatacg cttgataagt
     1201 agcttctgtg aacggatagt tcttttgcta aatcttgaat tgtaagtgat tgaggatagt
     1261 ggctatcaat taatcgtttg cattcaagat agagttggtg ggttgatgaa atattctttt
     1321 ttttctgatt gggagcaata gttcccagat gaaacatcag ttcatgaagt tgtcccatga
     1381 tatggagttg agctaattca cttgattttg taatctgagc gaagcggaca atgtctgaga
     1441 tgagttttgc agtagtctgg gtatgacaag tttcagattg gatgagatag gattgatcag
     1501 aaatttggga aagagcaaaa taatcagggg ctttccctcc agtgattcct aaccagtagt
     1561 aggcccaagg ttctttacta tctgcttgat aaaaggttag ttcctctggt tttaatagaa
     1621 agaaatctcc ttcttttaaa tcaacaattt tacccttgta atgaaatttt ccttgtcctt
     1681 tagtaatgta atgtagaacg tatgtatcac gaatggctgg accaaaagag taattaggtg
     1741 tgcattcctc atatccataa aagcttaggg caaggtcgat tgttccagtc tggtattctg
     1801 aaaaaactaa catgtgctac ctcctaccta acattttacc atattcttga gacatttttc
     1861 tattttggaa agcgatttca ggtgataaaa tataatcaat aaagtgatga ggtgaagtaa
     1921 atgggagtta ggatagagaa taatctattt tatgttgaga gtaaaaatct aagtttgatt
     1981 attgaaaatc gaaatggcta cttacttttg aaacatttag gaaagactat taagaactat
     2041 aaaggttcca atagtgttta tgaacgagac catgcctttt caggaaatcc aacggctact
     2101 aatcgaacct ttagtttaga tactcaacgt caaatttttg gacaacatgg cttaggagat
     2161 tttaggaaac caaccataca ggttcagcat aatgtaactg aagtaacaga ctttcgattt
     2221 gtagaagcaa agattttaaa aggtcagaat ggtccacagg gcttaccttc tccacatagc
     2281 atggacgata cagagactct tgtcttaatg ttagaagatt ctaaggctca acttagtctg
     2341 actttgtatt atactacttt taataatgat gcgactattg ctagctacag taaattagat
     2401 aataatagta atcaggaagt tgtcatccat aaagattttt cttttatggc tgattttcca
     2461 gctgcagatt atgaaatagc aactctgcag ggtgcttatg ctcgtgaaaa gactgttaga
     2521 cgtcaacagg tagaacaagg aatcttttcg attagttcaa accgaggtgc ttctggtcat
     2581 gctcaaacac cagctcttct actatgcgaa caaggagtca cagaggatgc tgggaatgtg
     2641 tttgctattc aactaatgta tagtggcaac tttgaagctt ttgttcaaaa aaatcaattg
     2701 aatgaagttc gggtggctat tggcattaat ccagaaaact tttcttggaa gttagcccct
     2761 gaggaatact ttgaaacacc ggtagcttta gtgactcatt cagatcaggg attaactggt
     2821 attagtcatg aaagtcagaa ttttgtactg aagcacatta tgctaagtga attttctaaa
     2881 aaagaacgtc caattctaat caataactgg gaagctactt actttgactt tcagagagaa
     2941 aaactgttag agttagcaga tgaagctaag aaagttggca ttgaactttt tgtattagat
     3001 gatggttggt ttggcaatcg ttttgatgat aatcgtgctt taggtgattg ggttgttaat
     3061 gaggaaaaac tgggtggaag tctagaaagt ctgatttcag ctatccatga aagaggtttg
     3121 cagtttggac tttggttaga acccgaaatg atttctgtag atagtgattt gtatcgtcaa
     3181 catcctgact gggctattca ggttcctgat tatgagcata cttattctcg gaatcaatta
     3241 gtacttaatc ttgccaatcc tcaggtagta gaatacttga aaagtgtctt agatcaactc
     3301 ctatcttatc atgagattga ttacattaaa tgggatatga accgcaatat cactaagcta
     3361 gggaatggat taacttatct agagacacag atgcaatctc atcagtacat gctggggctt
     3421 tacgaactcg tttcttatct gacagagaag cacagccata ttctctttga gtcctgctct
     3481 ggtggtggtg gacgaaatga tcttggtatg atacgctatt tcccacaagt ctgggctagt
     3541 gataatactg atgccattgc acgtttacca attcaatacg gttcatccta tctctatcca
     3601 accatttcta tgggggctca tgtgtcagca gtaccgaatc atcagatggg acgaatgaca
     3661 ccattagaaa cacgtggcca tgtagcaatg atgggaaatt tgggctatga gcttgatttg
     3721 acaaatttat cagatgaaga gaaagctacg attgctaatc aggtgaactt gtataaagaa
     3781 ttacgaccag tagttcagtt aggacagcag tatagactaa ttaatcctga tactgcatcc
     3841 aatgaagcag ctgtacaatt taattacgga aatcaaacga ttgtaaccta cgttcgcgtt
     3901 ttatctgttg tagagaccat ggaaacaact ttaaaattaa aagatttgga tgaagaggga
     3961 ctatataaat tacaggaaaa tggcgaagtt tactcaggtg cagaactaat gtatgcgggc
     4021 ttaactgtta tcttatccca aggagatttt ttgagtagac agtatatttt tagaaaacta
     4081 tgatatgtac tgaccccaaa agttagattt attctgtcta acttttgggg tgcagttcaa
     4141 taagggtgta taaatttata aaggaggctt tctaatggaa tggtataaaa aaatcggact
     4201 tcttgcaact acaggtttag ctttgtttgg gctcggcgct tgctccaact atggtaaatc
     4261 tgcggatggc acagtgacca tcgagtattt caaccagaaa aaagaaatga ccaaaacctt
     4321 ggaagaaatc actcgtgatt ttgagaagga aaaccctaag atcaaggtca aagtcgtcaa
     4381 tgtaccaaat gctggtgaag tattgaagac acgcgttctc gcaggagatg tgcctgatgt
     4441 ggtcaatatt tacccacagt ccatcgaact gcaagaatgg gcaaaagcag gtgtttttga
     4501 agatttgagc aacaaagact acctgaaacg cgtgaaaaat gcctacgctg aaaaatatgc
     4561 tgtaaacgaa aaagtttata acgttccttt tacagctaat gcttatggaa tttactacaa
     4621 caaagataaa ttcgaagaac tgggcttgaa ggttcctgaa acctgggatg aatttgaaca
     4681 gttagtcaaa gatatcgttg ctaaaggaca aacaccattt ggaattgcag gtgcagatgc
     4741 ctggacactc aatggttaca atcaattagc ctttgcgaca gcaacaggtg gaggaaaaga
     4801 agcaaatcaa taccttcgtt attctcaacc aaatgccatt aaattgtcgg atccgattat
     4861 gaaagatgac atcaaggtca tggacatcct tcgcatcaat ggctctaagc aaaagaactg
     4921 ggaaggtgct ggctataccg atgttatcgg agccttcgca cgtggggatg tcctcatgac
     4981 accaaatggg tcttgggcga tcacagcgat taatgaacaa aaaccgaact ttaagattgg
     5041 gaccttcatg attccaggaa aagaaaaagg acaaagctta accgttggtg cgggagactt
     5101 ggcatggtct atctcagcca ccaccaaaca tccaaaagaa gccaatgcct ttgtggaata
     5161 tatgacccgt ccagaagtca tgcaaaaata ctacgatgtg gacggatctc caacagcgat
     5221 cgaaggggtc aaacaagcag gagaagattc accgcttgct ggtatgaccg aatatgcctt
     5281 tacggatcgt cacttggtct ggttgcaaca atactggacc agtgaagcag acttccatac
     5341 cttgaccatg aactatgtct tgaccggtga taaacaaggc atggtcaatg atttgaatgc
     5401 cttctttaac ccgatgaaag cggatgtgga ttaggagtag agagactatg aaaaaagtat
     5461 tacaaaaata ttgggcatgg gcttttgtgg tcatccccct cttgttacaa gcaattttct
     5521 tctatgtgcc gatgtttcaa ggagcctttt acagttttac caactggaca ggattgactt
     5581 acaactacaa atttgtcggc ttaaacaact ttaagctcct cttcatggat ccaaaattca
     5641 tgaatgcgat tggctttacc gcaatcatta cgattgccat ggttggttgg tgagattgca
     5701 ctcgggatct tcattgcgcg tgtcttgaac tctaaaatca aaggccaaac cttcttccgt
     5761 gcttggttct tcttcccagc tgttttatct ggtttgacag tggctttgat cttcaagcaa
     5821 gtcttcaact acggtcttcc agcgattggg aatgcccttc atattgaatt tctccaaacc
     5881 agtcttttag ggactaagtg gggagcaatc tttgcggctg tctttgtcct tctttggcaa
     5941 ggggtggcta tgcccatcat catcttccta gctggtttgc aatctattcc aactgagatt
     6001 acagaggcag caaggattga tggtgcgact agcaagcaag ttttctggaa cattgaattg
     6061 ccttacttgc taccaagtgt ctctatggtc tttatcctag ccctaaaagg tgggctgact
     6121 gcctttgacc aagtctttgc catgaccggt ggtggtccaa acaatgccac aacctcactt
     6181 gggctcttgg tttataacta tgcctttaaa aacaaccaat tcggttatgc caatgccatt
     6241 gccgtaatct tgttcctctt aattgtagtg atttcgatca tccaattgag agtatctaag
     6301 aaatttgaaa tttaagagga gaagcatgat gaaacaagat gaaagaaaag ccctgattgg
     6361 caaatacatt ctattgattc taggatcggt tctgatttta gtgccgctcc ttgctaccct
     6421 ctttagttcc ttcaaaccca ctaaggatat tgtagataat ttctttggct ttccaaccaa
     6481 cttcacatgg gacaacttta gccgtctctt agctgatggg attggaggct attattggaa
     6541 ctctgtcgtc atcactgtct tgtctttact tgcagtaatg atctttatcc ctatggcagc
     6601 ctactccatc gctcgcaata tgagtaaaag aaaagtcttt accatcatgt ataccctctt
     6661 aatcctcgga atcttcgtac ctttccaagt catcatgatt ccgattacgg ttatgatgag
     6721 taaactcggt ttggctaata cctttggttt gatcttgctc tacttgacct atgcgattcc
     6781 acagaccctc tttctctatg ttggctatat caaaatctcg attccagaaa gtctggatga
     6841 agcagcagag atcgatgggg ctaatcaatt tacaacctat ttccgcatca tcttcccaat
     6901 gatgaaaccg atgcatgcga caaccatgat catcaatgcc ctttggttct ggaatgactt
     6961 catgttgcca ctccttgtct tgaaccggga ttccaaaatg tggactctgc ctttgttcca
     7021 atacaactac gcaggccaat atttcaacga ctacggacca agctttgcct cttacgtggt
     7081 cggcattatc agtatcacca ttgtctatct cttcttccaa cgccatatca tttcaggaat
     7141 gagcaacggg gcagtgaagt aatactcaat gaaaatcaaa gagcaaacta ggaagctagc
     7201 cgcaggttgc tcaaaacact gttttgaggt tgtggatgaa actgacgaag tcagtaacca
     7261 tacctacggt aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt ataagaagag
     7321 ctcatggaat ctatagtctt tgacacagag gaagagttca gcttgttgat aattctatgt
     7381 agaaaacaag aaagtagtac tttgtttatc acagtctata ttagtacgag tttgatgata
     7441 aatagagatt taaaatgaat ttgatttacg aagccagatc atatgagact tggcttcaat
     7501 tagaaaaagg agagtaatga tgccaattca aaataaaacc atgttgatta cctattctga
     7561 tagccttgga aataatctta aagacttata tgataatttg gaagagcatt ttggagatgc
     7621 tattggagga gttcaccttt taccattttt cccatcaaca ggtgatcgtg gatttgcgcc
     7681 agttgactac gacgaagtgg actcagcttt tggtgattgg gaggatgtta agcgtttagg
     7741 tgagaaatat tatcttatgt ttgattttat gattaatcat atttctcgtc aatccaagta
     7801 ttataaggac tatcaagaaa aacatgaagc cagtgaattt aaagatctct ttttaaactg
     7861 ggataagttt tggccagaaa accgtccgac acagtctgat gtagatttaa tttacaagcg
     7921 taaggatcgt gcaccaaagc aagagattgt gtttgaagat ggttcagtgg aacatttgtg
     7981 gaataccttt ggtgaggagc agattgatct tgatgtgacc aaagaagtaa ctatggaatt
     8041 tatccgtaag accattcagc acttggcaag taatgggtgt gatttgattc gtctagatgc
     8101 ctttgcttat gcagtgaaga aattggatac taatgatttc tttgtggaac cagatatttg
     8161 ggatttattg gacaaagttc gagatatcgc tgctgagtat gggacagagc ttttacctga
     8221 gattcatgaa cactattcga ttcagtttaa aatagcagac catgattact atgtttatga
     8281 ttttgctctt ccaatggtga cactttatac tctttacagt tccagaacag agcgtttggc
     8341 taagtggtta aagatgagcc cgatgaagca atttacgacg ctagataccc atgatgggat
     8401 tggagtagta gatgtcaagg atatcctgac cgatgaggag attgactatg cttcaaatga
     8461 actctataag gttggagcca atgtcaaacg taagtactct agtgccgagt ataacaactt
     8521 agatatctac caaatcaatt caacctacta ttcagcgctt ggagatgatg atgtcaagta
     8581 ttttctcgct cgtctaattc aagcttttgc cccaggtatt cctcaggttt actatgtggg
     8641 tctattagca ggcaagaatg acttgaaatt attagaagaa actaaagaag gtcgaaatat
     8701 taatcgtcat tactatagca acgaggaaat agcaaaagaa gtgcaacgac ctgttgtgaa
     8761 ggcccttctc aatctatttt ctttccgtaa ccgttcagaa gcctttgatc tagaagggac
     8821 tactgagata gagacaccaa cagctcacag cattgtaatc aaacgtcaaa ataaagataa
     8881 gtccgtaaca gcagtagcag aaattgactt gcaaaatcag acttatcggg taattgagaa
     8941 tggagttgaa gtaacatttt gaagccttga tatggtggat aaaatagggt ttttattttg
     9001 gaaaacgttt cctttgtttt caaattgcta aaaaagtggt acaatatagg atagcttact
     9061 attatctgaa tcagctgatt tggagagaaa ggattcattt tgaaatcaat aggctttatt
     9121 gaaaagctga aggggttgtc tagtaaagag ctgattttat tgggaattat cctgagtatc
     9181 tttttaccct tttatctttt tgtagttgta ttctgtttat atattatcag tctgattttt
     9241 acaggagaca tgaaaagtat tcttcagaaa atgggggagc attcgatgct gcttcttttt
     9301 cttggctata gtactgttat atccattctt gcacaaaatt ggatgggtct tgtggcttca
     9361 gtaggaatgt ttctatttac tattttcttt ttgcactatc agtcgatttt atcccataaa
     9421 ttctttcgat tgattttgca gttcgtcttg tttggtagtg tcttgtcagc tgcttttgcc
     9481 agtttagaac atttccaaat tgtgaagaaa tttaactatg cttttctttc ccccaatatg
     9541 caggtgtggc atcagaaccg ggcagaagtg accttcttta atcctaatta ttatggaatt
     9601 atttgttgtt tctgtattat gattgctttc tatctgttta caacgaccaa gttgaattgg
     9661 ttgaaagtat tctgtgtgat tgcaggcttt gttaatctct ttggtttgaa ctttactcaa
     9721 aatcgaactg cctttcctgc tattatcgct ggagcaatta tctatctctt tacgactatt
     9781 aaaaactgga aggccttttg gcttagtatt ggggtcttcg cgattggttt gagtttcctc
     9841 ttttctagtg atttgggagt tcgaatgggt actttagact cttctatgga agaacgcatt
     9901 tctatctggg atgctgggat ggccttgttt aagcaaaatc ctttttgggg tgaagggcca
     9961 ttgacctata tgcactctta tcctcggata catgctcctt atcatgaaca tgcccacagt
    10021 ctttatattg atacgattct gagttacgga attgtgggta ccattttatt agttctgtct
    10081 tctgttgctc ctgttcgctt gatgatggat atgagtcagg agtcggggaa acgtccgatt
    10141 atcggccttt atctatcttt ccttacagtg gttgctgtgc acggaatttt tgacttggct
    10201 ctcttctgga ttcagtcagg ctttattttc ttgctagtta tgtgcagtat tccattggag
    10261 catcgaatgt tggtatcgga catgacggat taagttttat aagtttgaaa tcttctacct
    10321 tgggtaggag attttttata cgatgggcat gtcgtgcatc tgaggtgtaa gtcctctgta
    10381 gggatccact accggtgaat acaatagcgc tccccaagcc tgactatcgt gaggtagcgg
    10441 attaaaatgg tctggggata gatcgtttta agtctgacgc aggaaataag aattgtcaga
    10501 gaaagcaata gtgaaatcgt aggctatgaa tagaaacgtg atagcgagga gcatatagtg
    10561 gataaggtag cgagaaattt taaagtataa aagatagtct taatctatat atgtaaatca
    10621 caaaagtaat tgaattcgga ataaggttgg tgtgaaaaga catagtaaac tgaatcttga
    10681 atactagaat gatacttcta caatattgtt ataatttaat ttgaattcca aaatcacatt
    10741 gttccgtccg tatttcattt tactgtatgt cttcctaaac tccaaagttt ctgcgtcaaa
    10801 tttcctattt tcactgtagt ctttgacact tcgaacgaca atttgaatca tatattcaaa
    10861 aaaaaatgaa aaatttgtgc caacaagcct aaatattctg aaaatttgtt tgatttttaa
    10921 tggataatgt gatataatgg ttgtcaatct ataattcaca taaacagagg agtagaaaat
    10981 gaaaaaaaat cgtgtatttg ctacagcagg tcttgtttta ttagcagcag gtgtacttgc
    11041 agcatgcagt tcttcaaaaa catctgattc atcagcccct aaagcttatg gctatgttta
    11101 tacagcagac ccagaaacct tggactacct gatttcaagt aaaaatagta caacagtagt
    11161 gacttcaaat gggattgatg gtttattcac taacgataat tacggtaatc ttgctcctgc
    11221 agttgcagag gattgggaag tctctaagga tggtttgacc tacacttata agattcgtaa
    11281 aggggttaaa tggtttacct ctgatggaga agaatatgca gaggtgacgg ctaaagattt
    11341 cgtgaacggt ttaaaacacg cagcagataa aaaatcagaa gctatgtatt tagctgaaaa
    11401 ttcggttaaa ggcttggcag attatctatc aggaacttca acagattttt caacagttgg
    11461 tgtcaaggcg gttgatgatt atacgttaca atacactttg aaccagcctg aaccgttctg
    11521 gaactctaag ttgacctatt ctattttctg gcctctgaat gaagaattcg aaacatcaaa
    11581 aggaagcgat tttgctaaac caacagatcc gacatccttg ctttataatg gtccattctt
    11641 gttgaaaggg ttgactgcaa aatcttctgt agagtttgta aaaaatgagc aatattggga
    11701 taaagaaaat gtccacctag atactatcaa tctagcttac tatgatggat cagatcagga
    11761 gtcgctagag cgtaacttca ctagtggagc ttatagttat gcccgtcttt accctaccag
    11821 ctccaactat tctaaggttg cagaagaata caaggacaat atctattaca cacaatcagg
    11881 ctctgggatt gctggtctgg gtgtgaatat tgatcgccaa agttacaact atacttctaa
    11941 aactacagat tcagagaaag tagctactaa gaaggcattg cttaacaaag atttccgtca
    12001 agccttgaat tttgctcttg atcgctcagc ttactcagct caaatcaatg gtaaagatgg
    12061 agcagcttta gcagttcgta atttatttgt aaaaccagac tttgtttcag ctggtgagaa
    12121 gacctttggt gatttagtcg ctgctcaact tcctgcttat ggtgatgagt ggaaaggtgt
    12181 gaatttagct gatgggcagg atggtttatt caatgctgac aaggccaagg cagagtttgc
    12241 gaaagctaag aaagctttag aagcagacgg cgttcagttt cctattcatc tggacgttcc
    12301 agtagaccaa gcatcaaaaa actatatatc tcgtattcag tcctttaaac aatctgtaga
    12361 aacagttctt ggtgttgaaa atgtcgttgt tgatattcaa caaatgacaa gtgatgaatt
    12421 ccttaatatt acttactatg ctgccaatgc ttcatctgag gattgggatg tatcgggagg
    12481 agtttcatgg gggccagact atcaagaccc atctacttac ctggatattt taaaaacaac
    12541 tagcagtgaa actacaaaaa catatttagg atttgataat ccaaatagcc cttcagtagt
    12601 tcaagttggt ttgaaagaat acgataaatt agttgatgaa gctgccaaag agacaagcga
    12661 cttgaatgtc cgttatgaaa aatatgcagc ggctcaagca tggttgacag atagttcact
    12721 ctttattcct gctatggctt cttctggtgc agcaccagtg ctttcacgaa ttgttccatt
    12781 tactggagct tctgcgcaaa caggctctaa ggggtcagat gtttacttca aatatttgaa
    12841 attacaagat aaagcggtga ctaaggaaga gtatgaaaaa gctcgtgaaa aatggttgaa
    12901 agaaaaagct gaatcaaatg agaaagctca aaaagaattg gcaagtcatg tgaagtaaat
    12961 aatttctaat agaactttct ctccatgagg agaaggttct tttgggattt ttaaaggaaa
    13021 taatatgaaa aaatatattt ttatgcgtgt tttgcggtca ttggtttcga ttttcttagt
    13081 aacgactttg acctacacga ttatctatac cttggttcct cgaaaattga ttttcaagca
    13141 ggatcctaac tataataaaa ttgcgacaac ggctgataaa cgtgataact atgaaaatac
    13201 tgtgtttgag cgtatgggct acattgagta ttacgatact aaagagttgc aagaaaaggc
    13261 aagtagcatg gattcttctg taacagtaga agcaaatgcg accaataaag ctatttatga
    13321 aaagtacatc aatcaattag gtcatggttg gactttggga gaatttactg aaagtggtca
    13381 attctatgct actcgtgaaa ttccaatttt tgaacgtgtt tttcacttct atgctaactt
    13441 gattgacatt gaccatacaa ataaaatcca agaccctgaa aatccagact tgaaacgcta
    13501 ccttcgtttt gaaaacgatc cagctatcgg atggtcattg gtcggttcag gaactaaaca
    13561 taaatatctc ttgtacttta acagtcagtt cccatttgtt catcaaaact ttgtgaactt
    13621 gaatttaggt gactcttacc caacctatgc taatacacca gttcttcagg ttattactca
    13681 aggtcaagga caaaccaaaa ctgcccaagt tcagttccca acaggtaaga aaacgtcttc
    13741 tgtaaatatt tactcaagaa cctacaagtc acctagtcag gctgactctc gtgaagtagc
    13801 tagctatggg aaagatgatc cttatacagc gactgaaagt aattaccaat atccatctat
    13861 gattgtcagc tctgctatta ctggtttgat tggtttggtt cttgcctatg ctcttgccgt
    13921 gccacttggt tcagccatgg ctcgtttcaa gaacacttgg attgatagcc tctcaacagg
    13981 ggctttgacc ttcttgcttg ctcttccaac gattgccttg gtttacatcg ttcgattgat
    14041 tggatcatct atttcccttc cagattcatt ccctatcttg ggagctggag attggcgttc
    14101 ttacgtttta ccagcagtca tccttggttt gttgggtgct cctggtacag ccatttggat
    14161 tcgtcgttac atgattgact tgcaatctca agactttgtt cgtttcgctc gtgcaaaagg
    14221 tttgtctgaa aaagaaattt caaacaaaca catctttaaa aatgccatgg ttccgctggt
    14281 ttcaggaatt cctggtgcca ttattggggt tatcggtggt gcaaccctta ctgaaacagt
    14341 cttcgccttc ccaggtatgg gtaaaatgtt gattgactct gtaaaagcat ctaataactc
    14401 tatggtcgtt ggtcttgtct tcatctttac atgtatttct atcttctcac gtcttttggg
    14461 agatatttgg atgactatta ttgacccacg tattaaattg actgagaaag gaggcaaata
    14521 atgtctacaa tcgataaaga aaaatttcag tttgtaaaac gtgacgattt tgcctctgaa
    14581 actattgatg cgccagcata ttcttactgg aaatcagtgt ttaaacaatt tatgaagaaa
    14641 aaatcaactg tagtcatgtt gggaatcttg gtagccatca ttttgataag tttcatctac
    14701 ccaatgtttt ctaagtttga tttcaatgat gtcagcaagg taaacgactt tagtgttcgt
    14761 tatatcaagc caaatgcgga gcattggttc ggtactgaca gtaacggtaa atcgctcttt
    14821 gacggtgtct ggttcggagc tcgtaactcc atcctcattt ctgtgattgc gacagtgatt
    14881 aacttggtta tcggtgtttt tgtcggtggt atttggggta tttcaaaatc agttgaccgt
    14941 gtcatgatgg aagtttacaa cgtcatctca aacatcccac ctcttttgat tgttattgtc
    15001 ttgacttact caatcggagc tggattctgg aatctgattt ttgccatgag cgtaacaaca
    15061 tggattggta ttgccttcat gatccgtgtg caaatcttgc gctatcgtga cttggaatac
    15121 aacttggcgt cacgtacttt gggaacacca accttgaaga ttgttgccaa aaatatcatg
    15181 cctcaattgg tatctgttat tgtgacaacc atgactcaaa tgcttccaag ctttatctca
    15241 tacgaagcct tcttgtcttt cttcggtctt ggattaccga ttacagtgcc aagtttgggt
    15301 cgtttgattt cggattattc acaaaacgta acaaccaatg cttacttgtt ctggattcca
    15361 ttgacaaccc ttgtcttggt atccttgtcc cttttcgtag ttggtcaaaa cttagcggat
    15421 gctagtgatc cacgtacaca tagataggag tagaaatgac aaaagaaaaa aatgtaattt
    15481 tgactgctcg cgatattgtc gtggaatttg acgttcgtga caaagtattg acagccattc
    15541 gcggcgtttc ccttgaacta gtcgaaggag aagtattagc cttggtaggt gagtcaggat
    15601 caggtaaatc tgttttgaca aagaccttca caggtatgct cgaagaaaat ggtcgcattg
    15661 cccaaggtag tattgactac cgtggtcagg acttgacagc tttatcttct cacaaggatt
    15721 gggaacaaat tcgtggtgct aagattgcga ctatcttcca ggacccaatg actagtttgg
    15781 accccattaa aacaattggt agtcagatta cagaagttat tgtaaaacac caaggaaaaa
    15841 cagctaaaga agcgaaagaa ttggccattg actacatgaa taaggttggc attccagacg
    15901 cagatagacg ttttaatgaa tacccattcc aatattctgg aggaatgcgt caacgtatcg
    15961 ttattgcgat tgcccttgcc tgccgacctg atgtcttgat ctgtgatgag ccaacaactg
    16021 ccttggatgt aactattcaa gctcagatta ttgatttgct aaaatcttta caaaacgagt
    16081 atcatttcac aacaatcttt attacccacg accttggtgt ggtggcaagt attgcggata
    16141 aggtagcggt tatgtatgca ggagaaatcg ttgagtatgg aacggttgag gaagtcttct
    16201 atgaccctcg ccatccatat acatggagtc tcttgtctag cttgcctcag cttgctgatg
    16261 ataaagggga tctttactca atcccaggaa cacctccgtc actttatact gacctgaaag
    16321 gggatgcttt tgctttgcgt tctgactacg caatgcagat tgacttcgaa caaaaagctc
    16381 ctcaattctc agtatcaaag acacattggg ctaaaacttg gcttcttcat gaggatgctc
    16441 caaaagtaga aaaaccagct gtgattgcaa atctccatga taagatccgt gaaaaaatgg
    16501 gatttgccca tctggctgac taggaggaag gaaatgtctg aaaaattagt agaaatcaaa
    16561 gatttagaaa tttccttcgg tgaaggaagt aagaagtttg tcgcggttaa aaatgctaac
    16621 ttctttatca acaagggaga aactttctcg cttgtaggtg agtccggtag tgggaaaaca
    16681 actattggtc gtgctatcat cggtctaaat gatacaagta atggagatat catttttgat
    16741 ggtcaaaaga ttaatggtaa gaaatcgcgt gaacaagctg cggaattgat tcgtcgaatc
    16801 cagatgattt tccaagaccc tgccgcaagt ttgaatgaac gtgcgactgt tgattatatt
    16861 atttctgaag gtctttacaa tcaccgttta tttaaggatg aagaagaacg taaagagaaa
    16921 gttcaaaata ttatccgtga agtaggtctt cttgctgagc acttgactcg ttaccctcat
    16981 gaattctcag gcggtcaacg tcaacgtatc ggtattgccc gtgccttggt catgcaacca
    17041 gactttgtta ttgcagatga gccaatttca gccttggacg tttctgtacg tgcccaagtc
    17101 ttgaacttgc tcaaaaaatt ccaaaaagag ctcggcctga cctatctctt catcgcccat
    17161 gacttgtcgg ttgttcgctt tatttcagat cgtatcgcag ttatttacaa gggtgttatt
    17221 gtagaggttg cagaaacaga agaattgttt aacaatccaa ttcacccata tactcaagcc
    17281 ttgctttcag cggtaccaat cccagatcca atcttggaac gtaagaaggt cttgaaggtt
    17341 tacgacccaa gtcaacacga ctatgagact gataagccat ctatggtaga aatccgtcca
    17401 ggtcactatg tttgggcgaa ccaagccgaa ttggcacgtt atcaaaaagg actaaactag
    17461 taatggtttt ataatttcca tgtcaatttt tatggaaatt ataaaacttt ttttgttgga
    17521 ctgatttgaa aaactttgaa agcttcctga aagaattttt gaaaaatttt aaaaattatg
    17581 aaatgttatt gacaggtgat gaaaaaggtg gtaggacagg aagcatcaaa aaagaaagca
    17641 gaaaaagaaa tgaataagag acaagccgta actatgaatc aaaactttta ctttagctac
    17701 tttagatagt gtttgtagac atgtcaccat ggatgcaaac agttcaagca atttgtttgt
    17761 atctatgtgg ctaagatttt tgattgtggt ccatatagat gaatatctaa atggactagc
    17821 taagttgtaa cttgttaggt tatttcaagt atccgtttag atagtatcta ggcggttttt
    17881 tgttttatct tttctgagaa ggagtttcat tatgaaagtt gttcgaaaat cactagcccc
    17941 ctcttggtag tggggctcct cttgacttct ctgatcagtt tgcatcagtt gaaggcagat
    18001 aagaaaaaga tgtctttcgt atcggtattt cgcaatttat tacccaccag tccttatacg
    18061 ctactagaga aggatttgtg gatgagctgg ccaaacaagg ctatgttgag ggggaaaata
    18121 ttgagattga tttgcaaaat gcacagggag aacaaagaaa tctaaaaacg atttcccagc
    18181 aactagcaga atccagcgat gtcgttctag ccatcgcaag gccttctgct cagagcttgg
    18241 ccaatacgac gcaaacgaca ccagttatct tttcagctgt aacagaccca gtcagtgcca
    18301 agttggttga gtcaagagaa catcctgggg gcaatgtaac tgggacaagc gatcagtcat
    18361 cagatgccat ttcaacccaa atcaatttga taaagaaagt gttgccaaag gctaaaacga
    18421 ttggaatcct ctatactcag agcgagccca attcggttgt tcaaaaggat gaagctaagc
    18481 gacttctgaa agaaaaaggc tttaccgttg ttgaaaaaac aatcttggac agtaataacg
    18541 tcaaggcggc agcagagagt ttgatggcag aggtggatat ggtttttgtc ccaactgata
    18601 atatcatttc gttaaccatg gaaacagtta agcaggtttc tattaaacac aaggttccag
    18661 tattcggtgg ttcaacagaa atgattgctg ttggtggctt gtataactac ggaaccaatt
    18721 atgaagaatt ggtacgtcag acagctcgca tgctgattcg tgttttaaaa ggtgagaaac
    18781 cagaaaatat agcagttgag ttgcctgaaa aactggaatt gcataccaat caagaaatgg
    18841 cagatgcatt gggaattgat attagtaagt tagaaggcaa ggaataagga ggattaagat
    18901 gaattttgtg ttatctagtt tatcagaagg tttgttgtgg tcgattgtgg cgattggggt
    18961 ctacttgact ttccgtattt tggatattgc ggatatgact gctgagggag cctttcctct
    19021 gggggcagca gtcgttgtat cacagataca ggcaggggca aatccttggc ttgcgacttt
    19081 acttgctttg ctggcaggta tggtagcagg tcttgtatca ggaatgctcc acaccaagat
    19141 gaaaatccca gctctcttga cagggattgt gaccttgaca gggctctatt caatcaatat
    19201 taaaatcatg ggcagtgtac ccaatctttc cttgggagat tctgcaaccg tctttaaaca
    19261 gttggcgagc ttgggtctga caaatgaagg aactgttttc tcacttagtt tggcctgttt
    19321 tttacttgtt tgtttggtct tgaccctttt gatgaaaaca gagattggcc tggtcttgcg
    19381 ttcgactggg gataatatcc cgatgagtga ggccaatgga gtcaatgtag acaccatgaa
    19441 gattgttggt tacatgattt caaacggttt gattgctcta tgtggttcct tgtttgccca
    19501 aaatgatgga ttttcagatg tgacttctgg gacaggaacc attgttgttg gtttgagctc
    19561 ggtgattatt gcggaagtct tgatacacga cttgaccatt ggaggccgct tattatccat
    19621 cggaatcggt gctattgttt accgtttgat tattttaaat atctatgaaa ttccaaatct
    19681 agatcaaaac ttggtacgtc tctttaatgc aatcttgctc gctttggttt tatttgcacc
    19741 agaattgcaa aagagattaa agattcgtgg tctgaaattg agaaatgaat aggaggagaa
    19801 agttatgaca agtttgttaa cacttgaaaa tattcacaag acatttgaag cagggacggt
    19861 caatgagaac catgtcctca aaggattaga tttagaggtt gaagagggag attttatctc
    19921 tgtgattggt ggaaatggag caggaaaatc cactttgatg aatatcttgg ctggcaatct
    19981 atcggtggac gaaggggacc ttctactggc agggaaatcc attaaaaatc tgagtgtcag
    20041 aaagagagcc aaggatattg cccgtgtttt tcaagatcct aagatgggaa cagcttctcg
    20101 tttgacgatt gaggagaata tggctattgc cttgagacgc gggcagaaga gagggcttgg
    20161 ttggggcgtg aaggagaagg atagaatcca gttccaagag gccttgaagg agttgaatat
    20221 tggtcttgaa aaccgcttaa aagtagatac tcaatatctc tcaggaggac aaagacaggc
    20281 tttgactcta gtcatgacag atctgatgaa accaaaactt ttacttttgg atgaacatac
    20341 tgcggcactt gacccgaaaa ccagtcaaat ggtgatggac ttgacgcaaa agattgtgga
    20401 gcaccatcag tggacgactt tgatgattac ccatgatatg aatcatgcga ttgagtacgg
    20461 caatcgtctc attatgctct atcaaggcaa gatagtggtg gatgtcaagg gagaagagaa
    20521 aaagcatctg acagttgaag accttatgca tctcttccag aaaaatagtg gccaaagtct
    20581 ggtcagtgat gaattagttt tgggataaag aaaaaggctg agatctggtg tctcagtctt
    20641 ttatttcttg cgttttgcaa aatctacaaa tctaaatttc tctaacttat ggcgactttc
    20701 ggtaaactga aattggcgtc cgtcttggag atagactttt gatttaatcg aaacgacata
    20761 agggtctttg ccaatgtcca tgagaatctt gtctcggtcg cttgagtggt caatggtgat
    20821 ttccttctga gcataatcaa taaggagttt gaggccattt tctatataag aatagataga
    20881 ctgctcagca atttggcgag tgagatttgg aattagttcc atatccagat agtccgtatc
    20941 cagaacggat accagatcat ccaccacacg ctggcggacc accttccaaa ccatccgaaa
    21001 ctctgggaaa ccggttatca gtgaggattt tttatcaata ataatcttgt ccagactgac
    21061 cacgttggtt ttagagcgca gtccaagttc tttaactagt tcttggtagc tggttaggtt
    21121 ggatacaggg aaattgacgg tttcttcttt gacgacttga gaaccttgct ctcttatctt
    21181 tttgatcaat ccttcctctt ggaggagaga cagggccttt cggacggtat cacgactcac
    21241 ttgatattgc tccataaggt cgtgctcgct aggaaggaaa tctccgacag cgtaagtctc
    21301 gttttgaatg gtttcttgga tttgcttaaa taattgttgg tatttcttca ttttgcctgc
    21361 tttaataggt ttttagtcta gtttaatgaa cacctgtatc tattttacca tacaagtgct
    21421 agaaaattta caaaaaaaca ccctttttat accaaacaag ttgcagacaa gtttggtatc
    21481 gtttacaata tacttatcaa atcaaacttg ctttgacaag tataaggaga atcaaatggg
    21541 aaaatttgaa caagaagcca aagatctgct tcaggcaatt ggaggcaaag aaaatgtgac
    21601 tgccgtaact cactgtgcga cacggatgcg ctttgtttta ggagatgata agaaggctaa
    21661 tgttaaagct atcgagtcaa ttccagctgt taaaggaact tttacaaatg caggtcaatt
    21721 tcaggtaatc attggaaatg acgtgcccat cttttataat gattttacag ccgtttcagg
    21781 tattgagggt gtttccaaag aagcagccaa gtctgcagct aagagtaatc aaaacgtggt
    21841 ccaacgtgtt atgaccactc tggcggagat ttttactccg attattccag ccttgatagt
    21901 cggaggattg atcctcggtt tccgtaatgt cttggaaggt gtccattggt cgatgttgga
    21961 tggcaagacc atcacagaat cctctcagtt ttgggcaggt gtcaatcact tcctctggtt
    22021 gcctggtgaa gctatcttcc agttcttacc agtagggatt acttggtctg tttctcgtaa
    22081 gatgggaacc agccaaattt tgggaattgt tctcggaatc tgtttggtat cgcctcagtt
    22141 gctcaatgcc tatgcggttg cttcaacgcc agcagctgat atcgcggcaa actgggtttg
    22201 gaattttggc tattttactg ttaatcgtat cggttaccaa gcccaagtta tcccagcctt
    22261 gcttgcaggt ttgagtctgt cttatcttga aatcttctgg cgcaagcata tcccagaagt
    22321 catttctatg atttttgtac ctttcttgtc attgattcca gccttgattt tggctcatac
    22381 tgttttggga ccaatcggtt ggacaattgg acaaggactt tcatcagttg tcttggcagg
    22441 tttaactggt ccagttaaag ggctcttcgg tgcaattttt ggtgccctct acgctccatt
    22501 tgtcatcaca ggtctgcacc atatgaccaa tgccattgat acacaattga ttgcggatgc
    22561 tggtggcact gccctatggc caatgattgc tctttctaat attgctcaag gctcagccgt
    22621 gtttgcctat tatttcatgc atcgccatga tgagcgtgag gctcaggttt cacttcctgc
    22681 aaccatttca gcctatctcg gtgttacaga accagctctt tttggggtta acgtaaaata
    22741 tatttatcca tttgttgctg ggatgactgg ttcagccctt gcaggcatgt tatccgttac
    22801 ttttaatgta actgcggctt ctattggtat cggtggtttg ccaggtattc tctctattca
    22861 acctcaatac atgctgccat ttgcaggaac tatgctagtt gcgattgttg ttccaatgct
    22921 cttgactttc ttcttccgca aggctggtct ctttacaaaa acagagggcg atacgaactt
    22981 gcaggcagaa ttcgttgctc aagaagaagc agaatttgtg aaccatgaac cagtagaact
    23041 tacttcggta gaaattatca gcccactaac tggccaagtg aaagaattga gtcaagcgac
    23101 ggatcctatt tttgcatcag gtgtcatggg gcaaggtctg gtcattgaac caagccaagg
    23161 tgagttgacc tctccagtta atgggacagt gacggttctt ttccctacca agcatgccat
    23221 cggcattgtc tctgacgagg gagttgaatt gctcatccac atcggtatgg atacagtagg
    23281 tcttgatggc aaaggttttg aaagtcttgt agtccaagga gatcacgtta cagttggtca
    23341 gcaactgatt cgttttgata tggatgtcat taaggctgca ggtctggtga cagaaactcc
    23401 tgttatcatc accaaccaag atgcttatac agcgactatt cccggaactt atccgacaac
    23461 gattcaagct ggagcatctc tcatggtcgc tacacgaatc taatactctt cgaaaatctc
    23521 ttcaaaccac gtcagctcta tctgcaacct caaaacagtg ttttgagcaa cctgtggcta
    23581 gcttcctagt ttgctctttg attttcattg agtataattg aaatagcaca aatagggacg
    23641 gaagactgct tcctcccttt tatcagaaaa ggagaaacag atgacacttg ataaaggaaa
    23701 agtagtctat caaatctatc caaaatctta caaagacacc actgaaaacg gtattgggga
    23761 tttccgtggg attatcgaaa aaattcccta tttagccaaa ctaggcgtgg atatggtctg
    23821 gctcaatcca ttctatccaa gccctcaacg ggataatggt tacgatattt cagattatat
    23881 ggcagtggat cctctttttg gtgatatggc tgattttgag gaaatggtgt gtgtcggtaa
    23941 agagcacaag attgacttta tgctggacat ggtacttaat cattgttcga cagagcatga
    24001 atggtttcag aaagccctag ctggtgataa gtattatcaa gactttttct tcatccaaga
    24061 ccaaccaaca gattggcagt ctaagtttgg tggttctgca tgggcgcctt tcggggatac
    24121 tgggaaatac tatcttcacc tatttgatga gacccaggct gaccttaact ggcgcaattc
    24181 caatgtccgt aaggagcttt tcaaggttgt taatttctgg cgcgacaagg gtgtcaaagg
    24241 tttccgattt gatgtgatca atttgatcgg taaggacgag gtttcagtgg actgtcctga
    24301 aaatgaaggg aagccagctt atacagacaa acccatcgtt cataactatt tgcgtatgat
    24361 gaaccaagcc acttttggat ctgacgatag ctttatgaca gttggggaaa tgtcttctac
    24421 caccatggaa aactgtgtcc tctattcgtc gcctgatcgt caggaattat ccatgacctt
    24481 taattttcat cacctcaagg tggactataa ggatggacaa aagtggacct tggctccctt
    24541 tgattttgaa gagttgaaga gtctttacca tagttggggt aaggaaatga gtgataaaga
    24601 cggttggagt gccctctttt ggaacaatca cgatcaacca cgtgccttga accgttttgt
    24661 cgatattcaa aacttccgca aggaaggagc caccatgcta gcagccagca ttcacctgtc
    24721 acgtgggaca ccttatatct acatgggtga ggaaatcggg atgattgacc cagactatga
    24781 ttctatggct gattatgtgg atgtcgaatc gcttaatgcc tatcagatgc tcttagaaga
    24841 aggaaagagt cagcaagaag ccttccagat tattcaagct aagtcgcgtg ataattcacg
    24901 tattcccatg cagtgggata cttctgaaaa tgcaggattt tcaacaggca ctccatggtt
    24961 gaaagtcggt aaatcctata aagatatcaa tgtagaaaat gaaatcaaag gtctgatttt
    25021 caccttctat caagacttga ttcgactgcg taaggaaatg ccaatcattt cagaaggaaa
    25081 ttacaaacca gcctttgaag atagcaaaca agtctacgct tttgaacgcc agtttgagga
    25141 tcaaaagtta ctagtgctca ataactttta tgccaaagaa gtggaaatcg acttgccagc
    25201 agtctaccaa aatggacaga ttttgatttc aaactatgaa gatgcagaag tgtctgagaa
    25261 aattctactc aaaccctatc aaacactggc tatttatgta aattaatact cttcgaaaat
    25321 ctcttcaaac cgtgtcagtt ttatctgtaa cctcaaagcg gtgctttgag caacctgtgg
    25381 ctagcttcct agtttgctct ttgattttca ttgagtataa acttagcaag gagacctctt
    25441 tttcagaaaa gcagggttct ttttgctgtc tattccatcc ttgaaagtgt agaacaaact
    25501 ggtcagggtg gctagtttgt ggtataatga aagggattca aaaagaaaca ggagaaaaat
    25561 gatggattta cttttagcaa ttgtattgat tgtgctagct tttctaggag gagctcttgg
    25621 aggaatgtac ttggttcgta agcaaatcga aaaagaattt gctgacaacc cacgtttgaa
    25681 tgctgaagca gttcgtactc ttttgagtgc aaatggtcaa aaaccaagcg aagctaaggt
    25741 acaacaagtt taccaccaaa tcatccgcca acaaaaggca gcccttgcta acaataaaaa
    25801 gaaaaaataa ataaggaaaa gtctgggatg aaagttccag acttctcact atgttggtta
    25861 tggtttaggg atgagacttt ttccttgcat tctattgata tttgattatg attaagagag
    25921 aggtagttga cattctatca agctgtcggt cagttcgttc agtacaagga atcataatga
    25981 acgatcaatc agaaaaaaga ctagaaagaa gactgtatgg ataatcgacc aattggtttt
    26041 ttggattcgg gtgtcggggg cttgaccgtt gtgcgcgagc tcatgcgcca gcttccccat
    26101 gaagaaatcg tctatattgg agattcggcg cgggcgccct atggcccccg tcctgctgag
    26161 caaattcgtg aatatacttg gcagctggtc aactttctct tgaccaagga tgtcaaaatg
    26221 attgtcattg cttgtaacac tgcgactgcg gtcgtctggg aagaaatcaa ggctcaacta
    26281 gatattcctg tcttgggtgt aattttgcca ggagcttcgg cagccatcaa gtccagtcaa
    26341 ggtgggaaaa tcggagtgat tggaacgccc atgacggtac aatcagacat ataccgtcag
    26401 aaaatccatg atctggatcc cgacttacag gtggagagct tggcctgtcc caagtttgct
    26461 cccttggttg agtcaggtgc cctgtcaacc agtgttacca agaaggtggt ctatgaaacc
    26521 ctgcgtccct tggttggaaa ggtggatagc ctgattttgg gctgtactca ttatccactc
    26581 cttcgcccta ttatccaaaa tgtgatgggg ccaaaggttc agctcatcga tagtggggca
    26641 gagtgcgtac gggatatctc agtcttactc aattattttg aaatcaatcg tggtcgcgat
    26701 gctggaccac tccatcaccg tttttacaca acagccagta gccaaagttt tgcacaaatt
    26761 ggtgaagaat ggctggaaaa agagattcat gtggagcatg tagaattatg acaaataaaa
    26821 tttatgaata taaggatgac caggactggt atgttgggtc ttatagtatt tttggtggcg
    26881 ttaacagttt gagcgactat aagacagatt ttcctctgtt tgaattctcc aaaatatttg
    26941 gagatgaaga gtatggtttc ccgctttcag ttactgtttt acgctatggt tctacctacc
    27001 gtttgttctc ctttgtggta gacatgctta atcaagaaat gggacgaaac ttggaagtta
    27061 ttcaacgtca tggggccctg ctcttggttg aaaatgggca actcttgtat gtagaattgc
    27121 ctaaagaagg ggtcaatgtt catgatttct ttgagacaag caaggtcaga gaaaccttgt
    27181 tgattgcgac tcgtaacgaa ggtaaaacca aggaattccg agctatcttt gataagttag
    27241 gctacgatgt ggaaaatctt aatgactacc ctgacctgcc tgaagtagca gaaacaggta
    27301 tgacctttga agaaaatgcc cgccttaagg cagaaaccat ttctcaatta acgggcaaga
    27361 tggttttggc agatgattct ggtctcaaag tcgatgtcct tggtggctta ccaggcgtct
    27421 ggtcagctcg tttcgcaggt gtgggagcaa ctgaccgtga aaataatgcc aaactcttgc
    27481 acgaattggc catggtcttt gaactcaagg accgctcggc tcagttccac acaaccctag
    27541 tcgtagccag cccaaataag gaaagtttag ttgttgaagc agactggtca ggttatatta
    27601 actttgaacc taagggtgaa aatggctttg gctatgatcc cctcttcctt gtaggagaga
    27661 caggtgagtc atcagctgaa ttaaccctag aagaaaaaaa tagtcaatct caccgtgcct
    27721 tagccgttaa gaaacttttg gaggtatttc catcatggca aagcaaacca tcattgtaat
    27781 gagcgattcc catggcgata gcttgattgt ggaagaagtc cgtgatcgct atgtgggcaa
    27841 agtcgatgcc gtttttcata acggcgattc tgaactacgt ccggattctc cactttggga
    27901 gggcatccgc gttgttaaag ggaacatgga cttctacgcc ggctacccag aacgtctggt
    27961 gactgagctt ggttcgacca agattatcca aactcatggt cacttgtttg acatcaattt
    28021 caactttcaa aagttggact actgggctca ggaggaagag gccgctatct gcctctatgg
    28081 tcacttgcat gtgccaagtg cttggttgga aggcaagatc ctctttctaa atccaggttc
    28141 tatcagtcaa ccacgaggta ccatcagaga atgtctctat gctcgtgtgg agattgatga
    28201 tagttacttc aaagtggact ttttgacacg agatcacgag gtgtatccag gtttgtccaa
    28261 ggagtttagc cgatgattgc caaggagttt gagactttct tgttggggca ggaggaaact
    28321 tttttgaccc ctgctaaaaa tctagctgtg ttgattgata cccacaatgc ggatcatgcg
    28381 accctcttgc tcagtcagat gacctatacc cgtgttcccg ttgtgacaga tgaaaaacag
    28441 tttgttggga cgattggact cagagatatt atggcttatc agatggagca tgacttgagc
    28501 caagaaatca tggcggatac ggatatcgtt catatgacaa aaacggacgt agcggttgtt
    28561 tcgcctgatt tcaccattac ggaggtcttg cacaagctag tagatgagtc cttcttaccg
    28621 gtcgtggatg cagagggtat tttccaaggg attattacgc gcaagtccat cctcaaggcc
    28681 gttaatgccc tcttgcatga ctttagtaag gaatatgaga ttcgatgcca atgagagaca
    28741 ggatttcagc ctttttagag gaaaagcagg gcttgtctgt caattccaag cagtcctata
    28801 agtatgattt ggagcaattt ttagacatgg taggtgagcg gatttctgag accagtctca
    28861 agatttacca agcccagcta gccaatctaa aaatcagcgc ccagaagcga aagatttcgg
    28921 cctgtaacca atttctatac tttctctatc aaaaaggaga ggtggacagc ttttaccgct
    28981 tggaattagc caaacaagct gaaaagaaga cggaaaagcc agagattcta tacctagact
    29041 ctttttggca ggaaagcgac catccagagg gccgcttgct agcgctctta atcctagaaa
    29101 tggggctctt gcccagtgag attttagcca tcaaggttgc ggacatcaat ctggattttc
    29161 aggtgctgcg aatcagcaag gcttcccaac agaggattgt caccattccc acggccttgc
    29221 tttcagaatt ggaacccttg atggggcaga cctatctttt tgaaagagga gagaaaccct
    29281 attctcgtca gtgggccttt cgtcagttag aatcttttgt caaggagaaa ggttttccat
    29341 ccttatcagc tcaagtctta cgtgaacagt ttattctaag acaaatagaa aacaaggtcg
    29401 atttgtacga aattgcaaaa aaattaggat taaaaacagt cctgacctta gaaaaatata
    29461 gataatggat attaaattaa aagattttga aggacccctg gacttgctct tgcatctggt
    29521 ttctaagtac cagatggata tctacgatgt gcccattacg gaagtcatcg aacagtatct
    29581 agcctatgtc tcaaccctgc aggccatgcg tctggaagtg acgggtgagt acatggtcat
    29641 ggctagtcag ctcatgctga ttaagagtcg taaactcctt ccgaaggtag cagaagtgac
    29701 agacttgggg gatgacctgg agcaggacct cctctctcaa atcgaagaat atcgcaagtt
    29761 caagctcttg ggtgagcact tggaagccaa gcaccaagaa cgggcccagt attattccaa
    29821 agcgccgaca gagttgattt acgaagatgc ggagcttgtg catgacaaga cgaccattga
    29881 cctctttttg gctttttcaa atatcctagc caagaaaaaa gaggagtttg cacaaaatca
    29941 cacgacgatc ttgcgggatg agtataagat tgaggacatg atgattatcg tgaaagagtc
    30001 cttgattgga cgagatcaat tgcgcttgca ggatttgttc aaggaagccc agaatgtcca
    30061 agaggtcatc accctctttt tggcaaccct agagttaatc aaaacccagg agctgatcct
    30121 cgtgcaagag gagagttttg gagatatcta tctcatggaa aagaaggaag aaagtcaagt
    30181 gcctcaaagc tagacttgat agagaggaaa gatgagtact ttagcaaaaa tagaagcgct
    30241 cttgtttgta gcgggtgaag atgggattcg ggtccgccag ttagctgaac tcctctctct
    30301 gccaccgaca ggcatccagc aaagtttagg aaaattagcc cagaagtatg aaaaggaccc
    30361 agattccagt ttggctttga ttgagacaag tggtgcttat agattggtga ccaagcctca
    30421 atttgcagag attttgaagg aatactctaa ggcgcctatc aaccagagct tgtctcgggc
    30481 tgcccttgag accttgtcca ttattgccta caaacagccg attacgcgga tagaaattga
    30541 tgccatccgt ggagttaact cgagtggagc cttggcaaag ttgcaggctt ttgacctgat
    30601 aaaggaagac gggaaaaagg aagtattggg gcgccccaac ctctatgtga ctacggatta
    30661 tttcctagat tacatgggga taaaccattt agaagaatta ccagtgattg atgagcttga
    30721 gattcaagcc caagaaagcc aattatttgg tgaaaggata gaagaagatg agaatcaata
    30781 agtatattgc ccacgcaggt gtggccagta ggagaaaagc agaagagctg attaagcaag
    30841 gcttggtgac ggttaacggc caagtggtgc gtgaactagc aaccactatc aagtcaggcg
    30901 acaaggtcga agttgaaggt caacctatct acaacgaaga aaaggtctac tatctgctta
    30961 acaaaccacg cggtgtgatt tccagtgtga cagatgataa gggtcgcaag acggttgtcg
    31021 acctcttgcc caatgtcaaa gagcgtattt accctgtggg tcgtttggac tgggatacat
    31081 caggtgtctt gattttgacc aatgatgggg actttacaga cgagatgatt caccctcgta
    31141 atgagattga caaggtttat gtcgcgcgtg ttaaaggtgt ggccaataag gacaatctcc
    31201 gccccttgac ccgtggtctt gagattgatg gtaagaaaac caagccagct gtttatgaaa
    31261 ttctcaaagt ggacccagtc aaaaatcgct ctgtggtgca gttgaccatc catgaagggc
    31321 gtaaccatca ggttaaaaag atgtttgaag ctgttggtct ccaagtagat aagttgtctc
    31381 ggactcgttt cggacaccta gacttgacag gactccgtcc aggagaatcc cgtcgtctta
    31441 ataaaaaaga aatcagccaa ctacacacca tggctgtaac taagaaataa tgaaacgaat
    31501 tttaatagcg cctgtgcgct tttaccaacg ttttatctca ccagtctttc caccctcttg
    31561 tcgctttgag ctgacttgtt ccaactacat gattcaggct attgaaaaac atggttttaa
    31621 gggggtattg atgggcttgg ctcggatttt acgttgtcat ccctggtcga aaacaggtaa
    31681 ggaccccgtt ccagaccact tttcccttaa acgaaatcaa gaaggggaat gaggtggggt
    31741 aaatagattt caaaatgata aaaacgcatc ctatcaggtt tgagtaaact tgataggatg
    31801 cgttttagaa tgtcaaaatt ttatactctt cgaaaatctc ttcaaaccgc gtcagctttc
    31861 atctgcaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgctcttt
    31921 gattttcatt gagtattaaa ttgagtttga agtggcttat ttcaaagctt tttgtatgtc
    31981 ttcaatcatg agttttgttg attcaagtcc gcctccgctt agataccaga ggtctggtgt
    32041 tagttggata atcttaccat ttttagcagc aggtgtttca gcgataaggg cattttctag
    32101 gacaccgtcg ttgctagagt tgtccccacc gatggcaagg gtacggttga tgacaaagag
    32161 gatgtcaggg ttgatttctt tgacactttc aaagctgact tcttgtccgt ggcgtgagtc
    32221 ttcaaatttt gtatcagttg gtttgaattt caaggtttgg tacaagaaag agaaacgaga
    32281 tttggcacca aaggctgcca tttttccttc attaaggagg atcgcaaggg cttttttgtc
    32341 agagctttca tttttagtag cgacttcttg gatgctcttg tctagcttgg ccaattcttc
    32401 cttggctttc tgtgtaccag tttcgccgaa ggcacttgct aaggattcga tattagcctt
    32461 ggtagaagtc cagtagtcgt ccttgcttgc ttggaagaga acggttgggg cgatttcttt
    32521 gaatttgtct acgaattttt gtgtacgtgg tgaagcgata atcaaatcag gctcaagggc
    32581 ggcgatagct tctaaatcag gttctttcat agaaccaaca tttttgacag ttcccactag
    32641 gtcttttaga taagtcggaa cagtttttgt aggcattccg acgatatttt tttcaaatcc
    32701 taaagcgcga atagtatccg cagcgccgag gtcaaaggtc acaatctttt caggaacttt
    32761 ggaaagtttg acctcggcca gtgaactttt aatggttacc tctgttggag cagagctact
    32821 ggtctctgtc tgactagtgc ttgagtttgt actacatgca ccaagtagga gcaagaagct
    32881 ggccactagg gcagtgaaat aaagtttaag ggatgttttc ataatttctc ctctttaaaa
    32941 tgtgataacg atttagggag tctcttgatc ttattgacta agagactgaa ggttctctaa
    33001 cttgagcttt tatgttacta gctatagata cagatctttt tgtcattgat atcagctagc
    33061 gtgatgggaa tctcataaag ttgactgagc aggtcagcct gcatgatttg atcggttctt
    33121 cccttgctaa agacctggcc gtccttgaag gcgacaattt catctgcata ctgactggct
    33181 atgttgatat cgtggaggac gatgataatg gtcttgccga gttcctccac cagtcgtcga
    33241 agaatctgca tcatgctgac gctttgcttg atatcgagat tgttgagtgg ttcgtccagc
    33301 aagataaagt ccgtatcctg ggccagtacc atagcgataa agacgcgctg gagttgcccc
    33361 cctgacaggc tattgatgta gcggtctttt aagttggtca gttccaaata gttcagagtt
    33421 tctcggattt tttcccagtc ttctgatcta agtcgacctc ggctgtaggg aaaacgtcca
    33481 aaactgacca gttcttcaac agtcaatttg gcttggtaat tgattttctg ttttaggatg
    33541 gttagttctt gggccagttc ttgcgaattc cagctctcga tttcacgtcc tttgatactg
    33601 agaactccct gatctttctt ggttagtctg ctcatgatgg agaggagagt cgattttcca
    33661 gcaccatttg gaccaataaa ggctgtcagt ttttgaggac tgacttcaag cgaaatgcct
    33721 tgcaaaatat cctgtttttg aatggatttg tcaatgtttt ccagtttcac tgacgagccc
    33781 tcctatatag taagataaag aataagaagc cacccacact ctcaatgatc atactgatac
    33841 gaatttccag tgcaaagact cgttcaatca aggcttgccc caaggttaag ctaataaatc
    33901 caaccagaat ggccactata aagagtaact tgtgctgata gtctttgaca atcaggtagg
    33961 tgaggttggc cagcataaag ccgaagaagg ccataggtcc taccaaggca gtggccgttg
    34021 aggtcaaaag cacgattccc cagaggagct ctttctgttc tttttcaaca tcgagtccca
    34081 atatctgagc cgtttctctt tgcaggtgca agacatctag aacgactgct tttcgaaaga
    34141 aaaagattgt caaagcgagg atgatcagag aaccgatggc taggatggaa gtgttgagat
    34201 gttgaaagga ggcaaaaaga ctattttgca gtttatcgta ttcgtttgga tccattagga
    34261 cttgaaggaa ggtgctgata tttcgaaaga gacttctgag cgctagacag atcagcagga
    34321 tgaagaccag gtcttgcttc atcagtgtct tcaagtaacc ttgtaaggcg agaaagaaga
    34381 gggactggac aagaagtaag actaggaatt ctaagatagg ggatttgcca agttgaagaa
    34441 acttgctttc aaaaaccagt agtagggttt gtagtaggac gtagaaggat tcaattccca
    34501 aaatactagg cgtcaggaag cgattttccg tcagggtttg aaaactaatg gtcgaaatcc
    34561 cagtcgcgat ggctaccaag agataaacga tgatcttttg ggaacgcaac ttccaagcaa
    34621 aggctgacaa gtgagtgatg ggccaaaagt agagaagaca agctccgatg gcaagaataa
    34681 tgagaatcca gaagagcttg gtatgtttgc ttttagtctg catcttttcg tccccctctc
    34741 cagagaagta ggataaagac gagactaccg atgattccta gcaagagact gacagacaac
    34801 tcatagggcc taatcagaac tcgggatagg atatcgcaag ccagaactag attggcacca
    34861 accagtgcga ccatgagttt ggtttgactt agattatctc catagcgctt gcgaacaaga
    34921 ttgggaacga taactccgag aaatggtagg ccacccacgg taatcatggt gacgcttgtc
    34981 gttagcgcca ccagaaagag ggccagtttt tcaagtaggg agtaggaaat ccccaaactc
    35041 tcgctggttt ctttccctag attcatgatg gtgaaggttt gggataattt ccaaacggtt
    35101 atcaggatga tgaggcctaa gaagagccac tcatactgat gggtctgaat catggagaag
    35161 gagccctggg tccaggcagt catactctga accagattga aacgataggc gataacttct
    35221 gtgactgagc cgataatccc gctatagatg atcccaatca gaggcaacat ccacctttcc
    35281 tttacagtaa aaatggtcat aaaggctagg aagaagaggg tgaatacgat ggatgaaaca
    35341 aaagcgaaga gcatcttttg ggtcagacta gccgatggaa agacaaaaag gctcagcacc
    35401 attcccagtt tggcggcttc agtcgttcca actgtactcg gtgcagcaaa ctgattttgg
    35461 gtaatagtct gcatgagaag gcctgccata ctcatactag aggcagtcag gagaatactg
    35521 atagttcttg ggagacggga ctcttgaaag aggagccagg tctgctggtc gaaatcaaat
    35581 agctttcccc atgaaaaatc actggtccca atgctaatag agagaaagac taggagtaga
    35641 agtaagccaa ttaaataatg agaaagtttc ataccccgtc ctttcatgta gatttggtat
    35701 cgaaagatat ctgcggatat aaatgtaaca ttatttttct aatctgtcaa taaaatttct
    35761 gacaatttaa taaatacaac aaggagagag caacaagact ttctcctttg ttatcctatt
    35821 ctaaaatgtt tttaccttaa tctgataaaa taatatcttc gagggagtag ctagccgtcc
    35881 aatcaagata ttgtttagct tttgaagcat ctgctaggac actggctggg tcactagcac
    35941 gtcgagcaac aatctcgtgt gggatttttt aatttagtaa ttcttcagca gttttaaaga
    36001 tttctttgat agtatagcct tttttagttc ctaagttaaa gatttgagaa gaactgtctt
    36061 cttgaaatag gtagttcatt cctttaacat gagcctatgc aaggtccaag acataaatgt
    36121 aatctcgaat acatgaaccg tcacgtgtat cgtagtcatc tccaaatatt tttaagctat
    36181 cattttgtcc caatgcggtc ttgttgatat ttggaatgat gtgagttgga tttttcacac
    36241 gcagaccgtt tgaagcatcc atttcagccc cagcaacatt aaagtaacgg aaaataacat
    36301 atttccagtc gtagcgattg gccatccagt aaatcattcg ttcgcccatc agttttgtct
    36361 ctgcataagg gttgacaggg tcgagcaggg tatcttcagt caccggcttg tcaatacagt
    36421 tatttccata gagagaagca gtcgaagaga acatgatttt ttgaatgcca acttcagata
    36481 agactttgag aacttggttc ataccagcaa cgttggcagt gaagtattta cttggatttt
    36541 caatactttc gctcacaaca atttcacctg cacaatggag gacagcatca atctgatttt
    36601 cttctaagta agcctttaag gcgctagcat cataaacatc cagttcttta aagctagcac
    36661 gactatccac agccgctcga tttcctgtgg agagattatc gaggacatgc accttatagc
    36721 cagcatttaa aagagcttta acggtatggg agccaatgta gctagtccca cctgtaacaa
    36781 gaattgtttt ggtcatgatt ttttcctttt ctagttttgt actatagtaa attgaaataa
    36841 gatatgaaca actctattag gaaagtcaaa ttaatttcta gaaatgtttc agcagctaca
    36901 gcgtactatt ccaaactcaa ccaactatat atttaaagaa agaagaaagg gaagtcaact
    36961 attttctgta aatttcatga aaaatccaaa caactttgat ggtgtattgt ttttttgttt
    37021 ggaaataaat attcgagtta attttggaat gtttactaga ttttgcacta catgcaagag
    37081 aatactatcc ttttctaaga tagagcagga ctaagatact aacttagaaa tccaccttcc
    37141 cactatcctt ctcctataaa aagtggtaaa atggatgaaa gaaagaagga actgaaatga
    37201 caacattatt ttcaaaaatt aaagaagtaa cagaacttgc tgcagtctca ggtcatgaag
    37261 cgcctgtccg tgcttatctt cgtgaaaagt tgacaccgca tgtggatgaa gtggtgacag
    37321 atggcttggg tggtattttt ggtatcaaac attcagaagc tgtggatgca ccgcgcgtct
    37381 tggtcgcttc tcatatggac gaagttggtt ttatggtcag cgaaatcaag ccagatggta
    37441 ccttccgtgt cgtagaaatc ggtggctgga accccatggt ggttagcagc caacgtttca
    37501 aactcttgac tcgtgatggt catgaaattc ctgtgatttc aggttctgtt cctccgcatt
    37561 tgactcgtgg aaagggggga ccaaccatgc cagccattgc cgatatcgtt tttgatggtg
    37621 gttttgcgga caaggctgag gcagaaagtt ttggcatccg tcctggtgat accatcgtac
    37681 cagatagttc tgcaattttg acagccaatg aaaaaaatat catctcaaaa gcttgggata
    37741 accgctacgg tgtcctcatg gtaagcgagc tagctgaagc tttatcaggt caaaaactcg
    37801 gcaatgaact ctatctgggt tctaacgtcc aagaagaagt tggtctgcgt ggcgctcata
    37861 cctctacaac caagtttgac ccagaagtct tcctcgcagt tgattgctca ccagcaggtg
    37921 atgtctacgg tggtcaaggc aagattggag atggaacctt gattcgtttc tatgatccag
    37981 gtcacttgct tctcccaggg atgaaggatt tccttttgac aacggctgaa gaagctggta
    38041 tcaagtacca atactactgt ggtaaaggcg gaacagatgc aggtgcagct catctgaaaa
    38101 atggtggtgt cccatcaaca actatcggtg tctgcgctcg ttatatccat tctcaccaaa
    38161 ccctctatgc aatggatgac ttcctagaag cgcaagcttt cttacaagcc ttggtgaaga
    38221 aattggatcg ttcaacggtt gatttgatta aacattatta aaccataagg gaggtggtag
    38281 ggatggtaga accaaaccta gaaagcctta taaaagatct ttacaatcat gctcgacatg
    38341 atttgagtga agatttagtt gctgctctcc tagagactac taaaaaactg cctactacaa
    38401 atgagcaatt gcaggcagtt cgtctctcaa gcctggtcaa tcgtgaattg ctcctaaatc
    38461 ccaaacatcc agcacctgag ttgctcaact tggctcgctt tgtcaaaaga gaagaagcca
    38521 agtacagagg aactgcgact tctgcgctta tgtatgagga actttttaaa atgctttgat
    38581 tccattgtgg atcaaagcat tttttataag acctatctcg tgagcgttca aatagtagta
    38641 acaaggaatc atgagaagtc agccgagacc atagtagtga agaaacttct gtaatggaag
    38701 tggagtgaag tgaggaatca tcaaacggta ggggataggc tgtttgaagg gtgctacgaa
    38761 aaagataggg taaggtgaaa ttatagcgga atgaaataag atatgaacaa attgattagg
    38821 gaaatcaaat gaatttatag aaatctttta gcagtcatgg cgtcctattc tagtttcaaa
    38881 atgctataaa tacgaacaaa ttgattggga aattcaaatc gctttctatc aatgttttag
    38941 aagatacagt gccctagtcc aacttcaata tactataaaa gaacagatgg aagcaattat
    39001 gatttacttc tatctttaaa ataaatgatc tgataggaaa aatgtgtccc ctcataaaaa
    39061 ataggatagt acggatggaa ggcttttgta gactggagga cgaaggaatt tagaaactct
    39121 cgttaaaagt tagttgagcc ggagtatggt ggtgtgaata attatcaaaa attatttcca
    39181 aatcgaaata gttgacagtt agaacgatga gtgttacaat tattttattc atttcgatat
    39241 cgaaataaat tggaggtgtt atgaaagata gtcatttgct agcccatcat attcgtttgt
    39301 tgaatgggcg gatttttcaa aagttactga gtcaagatcc tgaggctctt tataggggtg
    39361 aacagggcaa gattttagcg gttttatgga atagtgaaac tggctgcgca actgcgacag
    39421 atatcgcgct tgcgactggg cttgcgaata atacgctgac gactatgata aaaaagctag
    39481 aggaacaaaa gcttgtaatt gttagtccgt gtggaaaaga caagcgtaag aagtatttag
    39541 ttttaacgga gttaggcaag ttccagaaag aagtggggca tcgtgtcagt cagaaattgg
    39601 atactatctt ttacaaagga ttttcagagg aagaaattca ccaatttgaa ggttttcaag
    39661 aaagaatttt ggcaaatctg aaagagaagg aaaatgaggt ttagaatatg tcacaacaag
    39721 tgaaaaatgc tcacaaccta tacatccatg ccattcaaga cggacgagtt gctgaggctc
    39781 aagcccagtc tgtaggggat acctatatcc aacattcgac aggtgttcct gacgggaaag
    39841 aaggatttgc agccttcttt gcagatttct ttgagcgtca tccagagcgt cagattaaga
    39901 ttgtccgcac cattgaggat ggcaatctgg tctttgttca tgttcatcaa tatctgaatg
    39961 gtggagaagc tcaatgggtg acgacggata ctttccgtgc ggatgagaat ggtcgtattg
    40021 ttgagcattg ggatgtcatt gactactatc gaacacctga aaatgaccaa ctagatcaaa
    40081 tttttggaga ttttgaaatc aaggatttgg acaagaaagc agaaaataaa aagctggttc
    40141 gccgtttctt gacagaaact ttccaaaatg gggagctaga gcagtggagt gattatgtgg
    40201 cagacgattt gattcagcat aatcatgaga ttggacaagg aagtgctgct tataaaaact
    40261 atgtggctga atatagtgtc acttttgact ttgttttcca actcttagga caaggaaact
    40321 atgtggttag ctatggtcag actcagattg atggcgttgc ttatgcccag tacgatatct
    40381 tccgtttaga gaacgggaaa attgtggagc attgggataa taaggaagtc atacctaagg
    40441 tagaagactt gactaatcga gggaagtttt aaattgagga caaagaatga ttgaatacaa
    40501 aaatgtagca ctgcgctaca cagaaaaaga tgtcttgaga gatgtcaatt tacagattga
    40561 gaatggggag ttcatggttt tagttgggcc ttctgggtca ggtaagacga ccatgattaa
    40621 gatgattaac cgtctcttgg aaccaactga tggaaatatt tatatggatg gcaagcgcat
    40681 caaagactat gatgagcgtg aacttcgtct tactactgga tatgttttac aggctattgc
    40741 tctctttccc aatctaacag ttgaggaaaa tattgctctc attcctgaaa tgaagggttg
    40801 gactaaggaa gaaattgctc aaaaaacaga agagcttttg gcaaaagttg gattaccagt
    40861 agctgagtat gggcatcgac ttccaagtga gctatctggt ggagaacagc aacgggtcgg
    40921 tattgtccga gctatgattg gtcagcccaa gattttcctc atggatgaac ccttttcggc
    40981 cttggatgct atttcgagaa aacagttgca ggttctgaca aaagaattgc ataaagagtt
    41041 tgggatgaca acgatttttg taacccatga tacggatgaa gccttgaagt tggcggaccg
    41101 tattgctgtc ttgcaggatg gagaaattcg ccaggtagcg aatcccgaga caattttaaa
    41161 agtgcctgca acagactttg tagcagactt gtttggaggt agtgttcatg actaatttaa
    41221 ttgcaacttt tcaggatcgt tttagtgatt ggttgacagc cctatctcaa catttgcagt
    41281 tgtcactttt gaccttgtta ctagctattt tgcttgcgat tcccttggct gtttatcttc
    41341 gctatcatga gaagttggcg gattgggtct tacagattgc agggattttc cagaccatcc
    41401 cgtctctggc cttgttggga ctctttattc ccttgatggg aattgggact ttaccggcat
    41461 tgacagctct agtgatttat gcgatttttc cgattttaca aaatactatt actgggctga
    41521 agggaattga tccgagtctt caagaggctg ggattgcctt tgggatgacc agatgggagc
    41581 gtctcaagaa gtttgaaatt ccacttgcca tgcctgttat catgtctgga attcggacgg
    41641 cagcggtctt gattatcggt acggctacct tggcgacctt gattggggca gggggactgg
    41701 gttcctttat cctgttggga attgaccgta ataatgccag tttgattttg attggggccc
    41761 tttcttctgc agtgctagct attgccttta acttcctact aaaagtaatg gaaaaagcga
    41821 aattgcggac gattttctct ggttttgcct tggtggcttt attactgggt ctgtcttata
    41881 gtccagccct tttggctcaa aaagagaagg aaaacttgat tattgctggg aaaataggcc
    41941 cagaaccaga aattttggct aatatgtata aattgctgat tgaagaaaat accagcatga
    42001 ctgcgactgt taaaccgaat tttgggacga caagtttcct ttatgaagct ctgaaaaaag
    42061 gtgatattga catttatcct gaatttaccg gtacggtgac tgaaagtttg cttcaaccat
    42121 cacccaaggt gagtcatgag ccagagcagg tttatcaagt ggcgcgtgat ggcattgcca
    42181 agcaggatca tctagcctat ctcaaaccta tgtcttatca aaatacctat gctgtagctg
    42241 ttccgaaaaa gattgctcaa gaatatggct tgaagaccat ttcggatttg aaaaaagtgg
    42301 aagggcagct gaaggcaggc tttacgcttg agtttaacga ccgtgaagat ggaaataagg
    42361 gcttgcaatc aatgtatggt ctcaatctca atgtggcgac tatgcagcca gctcttcgtt
    42421 atcaggcaat tcagtcaggg gatattcaaa tcacggatgc ctattcgact gatgcggaat
    42481 tggagcgtta tgatttacag gtcttggaag atgacaagca actcttccca ccttatcaag
    42541 gggctccact catgaaagaa gctcttctca agaaacaccc agagttggaa agagttctta
    42601 atacattggc tggtaagatt acagaaagcc agatgagcca gctcaactac caagtcggtg
    42661 ttgaaggcaa gtcagcaaag caagtagcca aggagtttct ccaagaacaa ggtttgttga
    42721 agaaatgatg ggacagagac tttaaacata ctgaactcaa cttccttaaa cgactagata
    42781 aaagaatgct cagacaattt cgtctgggct tttttgatgt gagaatgact gaatgtcact
    42841 taaaatggaa agaaagagaa aattttcatg attttctaaa attttactgt aaagacagtt
    42901 gactattcat aaaataggtg tataatattt tgtgaactac ttaacaaata agaggtttca
    42961 gctcatgtcg actaaggtta gaaatcttgt gtataggcag ttcattatct aatagagcgt
    43021 tgcgtccgaa agtctatcca gacacggctc tttaaaaaca aaaggagaaa tgatgcatac
    43081 ttatttgcaa aagaaaattg aaaatatcaa aacaacccta ggtgaaatgt caggtggtta
    43141 ccgtcgtatg gttgcggcta tggctgattt aggattttca ggaactatga aggctatctg
    43201 ggatgacctc tttgcccatc gtagttttgc ccagtggatt tatttgctgg ttttaggaag
    43261 ttttcctctc tggctggagt tggtttacga acatcgtatt gttgactgga ttgggatgat
    43321 ttgtagcttg acagggatta tctgtgtaat ctttgtatcg gaaggtcgag caagtaatta
    43381 tctttttggc ttgattaact ctgttattta ccttattttg gccctacaga aaggctttta
    43441 tggtgaggtg ctgacgacac tttacttcac agtcatgcag ccaattggac ttctagtttg
    43501 gatttatcag gcacagttta agaaggaaaa gcaggagttt gtcgcgcgta aactggacgg
    43561 caagggctgg acaaagtatc tttccattag tgtgctttgg tggttggcct ttggcttcat
    43621 ttatcagtct attggtgcca atcgtcccta tcgtgattca atcacagatg caaccaatgg
    43681 ggtagggcaa atcctcatga cagctgttta ccgtgaacag tggatattct gggcggctac
    43741 caatgttttt tcaatctatc tctggtgggg agaaagcctg caaattcaag ggaaatatct
    43801 aatttatctc attaacagtc tagttggttg gtatcaatgg agcaaggcag ctaagcagaa
    43861 tactgattta cttaactagg aaaagatgtt tgaaagtgct gttttgagat ttcgattaaa
    43921 acagatatag ttgataatca aggatttata gtatgaaaaa gaggatcggc gggtcctctt
    43981 ttgttgttga aaagataaaa aactcagtaa cctagaaata agacaactga gctttactct
    44041 atattcaatt tttaggaatg agaaggtcta gataaaattg gacaacttcc tggcctgtga
    44101 aatcttgacc ttttttgagc caccaggtca atgtctcgat aaagttggac acgaccaagt
    44161 gttggaggta agaagtaggc atattagggt gggcttcttt taaattatca gctagcacgg
    44221 aatagacatg gtgttctagc tctttatgga gttgacggag gaagtagtca tttttggaaa
    44281 atagcagact ggtgatatgg tcttggtttt tatgaaaatg gagaaagagg tgggcgaggt
    44341 agtcctcggt tgaaatggct tgctctcttt caaaaagatg atggaagagg tagcggcaga
    44401 gctggtccag aagaagctcc ttactttcat agtgacagta aaaggtggat cttcccacat
    44461 ctgcgagatc aatgatatcc tgaacagtag tggcctcgta gcccttagca ttcaaaagtt
    44521 gtataaaagc ttgatagatg gcttttttgg ttttgctgat acggcggtca atgttagtca
    44581 tatggacact taaggcaaat tgttcagaac tgaataaagc tgacgttttg cttctatcct
    44641 ttctttgagt tttagtggat aatgataatg aacaaggtgt tcataaatct attataacaa
    44701 aggaatgaga aatatgaagg caaaatatgc tgtttgggtg gcttttttct taaatttgac
    44761 ttatgccatt gttgagttta ttgcaggtgg agtatttggt tctagcgctg ttcttgctga
    44821 ctctgtgcat gacttgggag atgcgattgc aattggaata tcagcttttc tagaaacaat
    44881 ctccaatcgt gaagaagaca atcagtacac cttgggctat aagcggttta gcctgctagg
    44941 agccttggta acagctgtga ttctcgtaac gggctctgtt ctagtcattt tggaaaatgt
    45001 cacgaagatt ttgcatccgc aaccagtcaa tgatgagggg attctctggt taggaattat
    45061 tgcgattact atcaatctgt tagcgagtct ggtggttggt aagggaaaga caaagaatga
    45121 gtctattctg agtttgcatt ttctggaaga tacgctaggg tgggtagctg ttatcctgat
    45181 ggcgattgtt cttcgattta cggactggta tatcctagat cctcttttgt cccttgtcat
    45241 ttctttcttt attctttcaa aagcccttcc acgtttttgg tctacactca agattttctt
    45301 ggatgctgtg ccagaaggtc ttgatatcaa gcaagtaaag agtggcctgg agcgattgga
    45361 caatgtggcc agccttaatc agcttaatct ctggactatg gatgctttgg aaaaaaatgc
    45421 cattgtccat gtttgtctaa aagaaatgga acatatggaa acttgtaaag agtctattcg
    45481 aattttccta aaagattgtg gttttcaaaa tattaccatt gaaattgatg ctgacctaga
    45541 aactcaccaa acccataagc gaaaggtgtg tgacttggaa cggagttatg agcatcaaca
    45601 ttagaaaaaa gtgaaaaata cttgggtact atcttatttg gaatagagta atttctttat
    45661 tatttaaata tttcaaaaat tggtaagaga agagcattgt ataaactccg gatatatgat
    45721 tgttaatgat aaaaattttt cgattagata caaaatgctt gacttggagt caactcaaag
    45781 ttatataata agataagtga gttagaatag cgtgaattca gtgaatgaaa tgagaggagg
    45841 ttagcgtgtg aatattaaat ctgccagtga tttgttggga atttcagcgg atacgattcg
    45901 gtattatgaa cgggttggtc ttgtgccacc gattactcgt actgctactg ggattcgtga
    45961 ttttcaagat caggatatcg aagcgctgga atttattaag tgttttcgtt cggcgggtgt
    46021 ctctgtagat agtttagttg actatatgtc gctctaccaa aagggagatg aaacgagaga
    46081 ggagaggctt ggtattttag aagaggaaaa gcaaaaatta gaggagcgct tgtctcagct
    46141 acagacagct ttaaatcgtt taaatctcaa aattaaactt tataaggaag gaaaatttta
    46201 aatgaaatca gcagtatata caaaggcagg tcaggttgga cttgctagca ttgaacgtcc
    46261 gcaaataata gaagcggatg atgtgattat tcgtgtggtt cgtgcgtgcg tttgtggttc
    46321 agatttatgg aggtaccgta atccagaaac gaaagctgga cataaaaata gtggacacga
    46381 agcgattggg attgttgaag aagctgggga agccattacg acggtgaaac caggtgattt
    46441 tgtgattgtc ccttttacac atggatgtgg tgagtgtgat gcctgtcttg ctggatttga
    46501 cggttcttgc gacaatcata ttggcaataa tttggggggt gattttcagg cagaatatat
    46561 tcgcttccac tatgcaaact gggcgctggt taaaatccct ggtcaacctt ctgattatac
    46621 agaagggatg ctcaagtccc ttttgactct tgcagatgtc atgccgacag gctatcatgc
    46681 agcgcgtgtt gcaaatgttc aaaaagggga caaggttgtt gttatcggtg atggggctgt
    46741 tggtcaatgt gctgtcatcg cggctaagat gcgtggagca tcacaaatta tccttatgag
    46801 ccgtcatgaa gaccgtcaaa agatggctat ggagtcaggt gcgacagctg ttgttgcaga
    46861 acgtggtcaa gaaggaatta ccaaggtgcg tgaaatcctc ggtggaggag cagatgcagc
    46921 acttgaatgt gttggtacgg aggctgctat agaacaggcg ctaggtgttc ttcataatgg
    46981 agggcgtatg ggctttgtag gagtcccaca ctataataat cgtgctcttg gttcgacatt
    47041 tatgcaaaat atctctgtag caggtggggc agcttctgct acaacatacg ataagcaatt
    47101 tttactaaaa gccgtccttg atggtgatat caatccaggt cgcgtcttta cttcaagtta
    47161 taaactggaa gatatcgacc aagcctacaa agatatggat gaacgtaaga ccattaagtc
    47221 tatgattata atcgaataaa aaacgaatag gagttctaaa actcctattc gttttttatg
    47281 ttatcctatt cttgatttag ggtactttct cttaatgtca gtctggttcc cagcatggtc
    47341 aggctaggga ttttccgacc gtggaggact tccttgttaa gaatatccat acctgctcgg
    47401 cccatttctt cagtataaac tgtaatacta gagaggggag gatagacctg tttggtcaga
    47461 ctagtgtcgt taaaggaaat gaggctgacg cgatctggca ggttgattcc agcttcttgg
    47521 agggcacgga gggcaccgat agctaaacta tcgctggctg cgaaaaatgc tggcggaagt
    47581 tggtctccca agctctgaat ggcctccttc atcaagtcat agccagactg ggcagtaaag
    47641 tttccttgaa agatcagttc atcatgatag attccttttg cttgactgta gtttctgaaa
    47701 ttttccagcc gcttatcctc aatgatttct tcttggtctg ttgtttcttc aaggcctgtt
    47761 agaatcccga tacggtccat tccttgactg aggaaataat cgacaacctg tttcatagca
    47821 gtgtaaaaat ccgtgataat acaggtatgt cccagggaaa gtgtatcgct gtctagaaat
    47881 acaagaggct tttggtattc ttcaaaggca gaaatctgag ctcgactaaa ctttccgatg
    47941 cagagaatcc caatcacttc ctcgcttagg gtaaaagggt ggtcattaaa atagcgcaag
    48001 atatcgtagt ccaactcttg ggctcttttt tctattccta ggcgaatctg gtagtagtag
    48061 aggtcgtcta gctccccttg ttcgctgacc cattgaataa tggcaatctt ttgcttgggt
    48121 ttgtgggact cgcctgtctt gaggtgcttg gtgtagccca gctcttcagc aacggttaaa
    48181 atacggtgtc tggtttcttc tgtaacagat aggctctggt cgcggttgag gacacgggat
    48241 acggtcgcga ttgagacaga ggctagctgt gcaatgtctt ttaaggtagc cataaatcct
    48301 ccttgattag gttagtatat catatttttc ttctttttac tgatatttta gtaaaatttt
    48361 agtaaaaagg attgaccttg ggaaattcct tggatacaat agaaagaaaa cgattacacg
    48421 ttaagatggc ttaacggaca gtcaaaggag aattcatatg gcacaacatc ttactactga
    48481 agtccttcgc aaagactttc ttgctgtttt tggtcaagaa gcagaccaaa ccttcttttc
    48541 accaggccgt attaatttga ttggtgaaca cacagactac aacggtgggc acgtttttcc
    48601 tgctgctatt tccttgggaa cttacggtgc agctcgtaag cgtgacgacc aagtcttgcg
    48661 tttctactca gctaactttg aggacaaggg cattatcgaa gtgcctctcg ctgacctcaa
    48721 gtttgaaaaa gagcacaact ggaccaatta tccaaaaggg gttcttcatt tcttgcaaga
    48781 agctgggcac gtgattgaca aaggttttga tttttatgtt tatgggaata tcccaaatgg
    48841 tgctggcttg tcttcttcag catccttgga gctcttgaca ggagtcgtgg cagagcatct
    48901 ctttgattta aaactagacc gtttggattt ggttaaaatc ggaaaacaaa cagaaaacaa
    48961 ctttatcgga gtcaactctg gtatcatgga ccagtttgct attggtatgg gggctgacca
    49021 acgtgctatt tacttagata ccaatacttt agaatacgac ttggtgccac ttgatttgaa
    49081 ggacaatgtc gttgttatca tgaacaccaa caaacgccgt gaattggcgg actctaaata
    49141 caatgaacgt cgtgctgagt gtgaaaaagc agtggaagaa ttgcaagttg ccttggatat
    49201 tcagaccctg ggtgaattgg acgagtgggc cgttgaccaa tatagctatt tgattaaaga
    49261 tgaaaatcgt ttgaaacgtg ctcgccatgc tgtgcttgaa aaccaacgta cccttaaagc
    49321 tcaagcagcc cttcaagcag gtgatttgga aacatttggt cgtttgatga atgcgtcaca
    49381 cgtttctctg gagcatgatt atgaagtaac tggtttggaa ttggatactc ttgttcacac
    49441 agcttgggca caagaaggag ttctcggtgc tcgtatgaca ggggctggtt ttggtggctg
    49501 tgccattgcc ttggttcaaa aagatactgt tgaggccttt aaggaagctg taggcaaaca
    49561 ctacgaggaa gtagttggat acgctccaag cttctatatc gctgaagttg caggtggcac
    49621 tcgcgtcctt gactagtcaa aaggaggctc tatagtgatc ttagtagata aatttgtaac
    49681 acatgtcatt tctgaaagtt catttgagga aatggatcga atctacctga ccaaccgtgt
    49741 tttggcacga gtgggagaag gtgttttgga agttgagacc aatctggata aattgattga
    49801 cctcaaggac cagctagttg aggaagccgt tcgattagag atgattgagg atagtcagac
    49861 tgcgcgtgaa atccttggta ctgaactgat ggacttggtg actccttatc cgagtcaggt
    49921 caatcgtgac ttctgggaag cctatgtcca ctctcctgag caagcgatag aggattttta
    49981 tcaactcagt cagaaaaatg attacatcaa actcaaggcc attgctaaaa atatcgctta
    50041 tcgtgttcca tctgattatg gagaacttga aattaccatc aatctctcta agcctgaaaa
    50101 ggatccgaaa gagattgcgg tagccaagtt ggtgcaagct agtaattatc ctcagtgtca
    50161 actttgttta gagaatgagg gttaccatgg tcgggtcaac cacccagctc gcagcaacca
    50221 ccggattatc cgttttgaaa tggttggtca ggaatggggg ttccagtatt cgccctatgc
    50281 ttactttaat gagcattgta tctttttaga tggccagcat cgtcccatgg ccattagtcg
    50341 tcagagtttt gaacgtctgt tggctatcgt agagcagttt ccaggatatt ttgctggatc
    50401 taatgccgac ctgccgattg tggggggctc tattctaact catgatcatt atcagggagg
    50461 ccgtcacgta tttcctatgg aattggctcc cttgcaaaag accttccgat ttgctggttt
    50521 tgagcaggtc aaggctggaa ttatcaagtg gccaatgtct gtcctacgct tgacttcgga
    50581 ttccaaagag gatttgatta acttagctga taagattttc caggaatggc gtcagtattc
    50641 agattctagt gtgcagattt tagcagagac agacgggaca ccgcatcaca ccatcacacc
    50701 aatagcgcgt aagcgtgatg gacagtttga gttggacttg gtcttgcgag acaatcagac
    50761 ttcagcagag catcctgatg gcatctatca tccccacaag gatgtccaac atatcaagaa
    50821 ggaaaatatc ggcttgattg aggtcatggg cttggcaatc ttgccaccac gtctgaaaga
    50881 agaagtagag caagtcgcta gctatcttgt aggagaagct gttacagttg ccgattatca
    50941 tcaggagtgg gcagaccaac tcaaatccca acatccagac ctaacggata aagaaaaagc
    51001 ccttgcaatc gtcaaggact ctgtgggtgc tatctttgtg cgtgtacttg aggatgcagg
    51061 agtctacaag cagacagaac aagggcagac agcctttatg cgctttgtgg aacaggtcgg
    51121 aattttacta gactaggagc tttctccttg cacagtccta taaaaagtag tatcatagtg
    51181 tcgtttgaaa tatcaggagg aaacgatgaa agactttcat tttgacgcta tatctgcctt
    51241 tgaaagttac gaaattgaaa aaatgagaga tggtcacgtt gtggtgacga ccaaagtagt
    51301 gaactcatcg ctcaactact atggcaatgc ccatggtggc tatctattca cgctatgtga
    51361 ccaaatcagt ggtttggtgg ttatctcgct gggacttgat ggggtgacac tccaatcctc
    51421 tatcaactac cttaaggcag gaaaactcga cgatgtattg accattaaag gagaatgtgt
    51481 ccatcaaggt cgtacaacct gtgtgatgga tgtggatatc accaatcaag agggcagaaa
    51541 tgtctgtaag gcaaccttta ccatgtttgt cacaggccaa aggtctgagg aaagacgggt
    51601 aagtatataa agaaaggtga gtgggataaa tttatctcac tcatttttaa tttatgagat
    51661 atgttagaag gaaatataaa agagagtgat gaaatacaaa tgtaataggt acatatatgt
    51721 ggtatactag ttatagtaga ttttaattgc ttatatcgag tgagttctca tattagtgag
    51781 gaatttttaa atggaattac actttaattt agaattagta gaaacctata aaagtaatag
    51841 ccaaaaggca cgtatcttaa cggaggattg ggtttatcgt cagtcttatt gccctaattg
    51901 tgggaacaat cccttaaatc attttgaaaa taatcggcct gtagcagatt tttactgtaa
    51961 tcattgtagt gaggagtttg aactaaagag caaaaaagga aatttttcat caacaatcaa
    52021 tgatggtgct tatgcaacga tgatgaagcg tgtgcaggca gataataatc ctaatttctt
    52081 ttttttaact tacacaaaaa attttgaggt aaataacttt cttgtccttc cgaagcaatt
    52141 tgttacaccg aaatcgatta ttcaaagaaa accacttgca ccaactgcta gacgagcagg
    52201 ttggattggt tgtaacattg atttatcaca agtaccttct aaaggaagga tatttcttgt
    52261 gcaagatgga caagttagag atccagaaaa agttacaaaa gaatttaagc aaggtttatt
    52321 tttaaggaag agctctctgt catcaagagg ttggacaata gaaattctaa attgtataga
    52381 taagatagag ggttcagaat ttacccttga agatatgtat cgttttgaaa gtgacctaaa
    52441 aaatatcttt gttaagaaca atcatatcaa agaaaagatt aggcaacagc ttcaaatatt
    52501 aagagacaaa gaaataatag aatttaaagg tagaggaaag tatcggaaat tatgaaaacg
    52561 aaacaacttg ttgcatcaga agaggtgtat gatttcttaa aagtcatctg gcctgattat
    52621 gaaactgaaa gccgttacga taacctaagt ttaatcgtct gtaccttatc agatcccgat
    52681 tgtgtgagat ggttatctga aaatatgaaa tttggtgacg aaaaacaact agctttgatg
    52741 aaggaaaaat atgggtggga agtaggagat aaattgccag agtggctaca tagctcctat
    52801 catagattat tgttaatagg tgaattattg gaaagcaatc taaaactgaa aaagtataca
    52861 gtagaaatta cagaaacttt atcacgttta gtaagtatag aggctgaaaa tccagatgaa
    52921 gccgaacgac ttgtaagaga aaagtataag agttgtgaaa ttgttcttga tgcagatgat
    52981 tttcaggact atgacactag catatatgaa taggtagatg tttttatttt gtcacacaaa
    53041 aaagaggctc gcacctcttt ttcttatttc tttttatgat ttaatacggc attgaggaca
    53101 atagcgagta ggctggctac gacgattccg tttgagaaga acatttggaa ggctgtcggc
    53161 atgctgacaa agagattact gttgttgaga ccgacacctg cagcgattga aacagctgcg
    53221 ataaggaagt tgtgttcatt gttagcaaag tcaactcggg cgaggatttg catcccttga
    53281 atagatacaa aaccaaacat caccagcatg gcaccgccga ggacggagct tggaatgatt
    53341 tgggcaaggg caccaaactt aggaagcagt ccaaggagaa ccaggaaacc agctgcgtag
    53401 tagattggca ggcgtttttt gatgcctgac aatttaacca aaccaacgtt ttgtgaaaat
    53461 ccggtgtaag ggaaggtgtt aaagattcct ccgagaagta ccgccaaacc ttctgcgcgg
    53521 taaccgttgc gaaggcgcgt gctgtcgatt ggatcttttg tgatatcaga caaggctaga
    53581 taaacaccag ttgactcaac catagacacc gttgcgatga tacacatcat gacaatagat
    53641 gagatttcaa aggttggcat cccaaagtag agtggagttg ggacatggac aagtggagct
    53701 accgcaacag gagagaagtc caccaagccc atagtagcag caatggcagt tccaacaacc
    53761 agaccaatca aaatagagat agacttgata aatcctttgg taaagatgtt gatcaagagg
    53821 ataatcagaa cagtaatagc tgcaagcaag agactttgac cagttggctc tggaacgtta
    53881 tttcccatat ttccaatagc gacagggatc aaggttaaac caatcgtggt aataacagat
    53941 cctgttacga tagatgggaa gagattggct acttttgaga agatgcctga aacaagaacc
    54001 acgtaaatcc cagatgcgat aagggcacca aacatagcgc cattaccatg gctttgccca
    54061 atcataatca agggagcgac cgactggaat gcaactccaa gaacgactgg gagtccaatc
    54121 ccaaagtatt tgttgagttg gagttggagg aaggttgcca ccccacacat gaagatatct
    54181 gtagaaatca ggtaggtcaa ctgctcagct gaatagccaa gggctgtcgc aatcatgatg
    54241 ggaaccagga tagatcctga gtacatggct agtaagtgct gcaagcccag aacggctgct
    54301 tgcgagtgtt tttcttgagt ttgcattaga gatctgcctc cttaaatacg acctgaccat
    54361 tttcaaaacg atccaagcga gcaagtgata ggacagggta gcctgctttt tcaagcaaat
    54421 cacgaccatc ttggaaggat ttctcaatca cgataccgat agcttggact gtggcaccgg
    54481 cctgttcgat gatttgaatc aagcctttag cagcttggcc attagcaagg aaatcgtcga
    54541 taatcaaaac cttgtcctct ggtgagagga attttccagc gatagaaaca gtgctggtca
    54601 cctgcttggt aaaggagtag acttgagcag ttaagatgcc ttcgttcatg gtgatgttct
    54661 tagctttttt ggcgaaaatc atgggaacgt ttaaggcttc agctgtaaaa acggctgggg
    54721 caatacccga cgcttcaatg gttacgacct tggtaatgcc agtagcagca aatttttccg
    54781 caaaaacctt accaatctct cgcatcaagc taaagtcaac ttggtgggtt aaaaaggaat
    54841 ctaccttgag gatgttatca cccaagatat gcccatcctt gaggatgcgc tcttctaata
    54901 atttcataag acctcctaaa gtctaaaagt taatttactt gttgtttaaa tatttctata
    54961 gtgatccctt ttgctaatac tatatatttg ataaaactat tacgagcgaa gcgagtctta
    55021 tcaaatattt cccgttgtag tggtatcata gacaataatc ttgttattgt ctatgacggg
    55081 atttttgaga gtaaaatagt tcggggaact attttagcct aagcctagaa atgaaagagc
    55141 taggggctca aaaattaggg atgaaattcc ctggattcct gaaattattc acaggataat
    55201 ttcacctccc gtccgcacta attaagggaa atattaaaaa tattcaaaaa agacctactt
    55261 aatctctaag taagtcccct aaatagacat ggcaaaaacg gccatatctc actgctgact
    55321 tacttattgt taggtgttcc ggcaccttgt agaaacgtcg tgccaattca cgacataaac
    55381 aagtaaaacg atattcaatt ttaaataggc ttgagccaat gtttttattt tacactaaat
    55441 aactttagaa atcaactatt ttgttagtgt tttggtttaa aaagcgaaca aaaataagag
    55501 agggtgaaca aaaactccat tgtaagctaa catttatact aaatgaaaat caaagagcaa
    55561 actaggaagc tatccacaac ctcaaaacac tgttttgagg ttgtggatag aattgacaga
    55621 gtcagtatca tatacctacg gtaaggcgac gttgacgtgg cttgaagaga ttttcgaaga
    55681 gtattagaag atttttccat cataaaaggc atactatcaa gtttttagac acctgacaat
    55741 atgccttttt ctaactttaa agacttttcc caatttttat tattctactc gctaaatctt
    55801 aaaaaatagc catctggatc caaaactgca aatttatgag gatagatata gggatcactg
    55861 acacgaaact ttcttttggt caagagacga taaataggat agtttgcctc catcactttt
    55921 tgatagagtt ttggaacatc ctttatgcca aaggagagat tgactccacg accaaagggg
    55981 taggtcagtt cagctagttg atcctttgtt ccctcctcta acattagttg acactcttca
    56041 agagaaagag aaagttttct tctggacgtt agtattcaat cctaaaaccc agtaaaccac
    56101 agtagaagga ccgggactgt tcgatattcg atacaagaaa ctcgggaatg accgcattgt
    56161 agtccatata gaaaatcctt acaagtcaat ttccaagaca atcggtgtat ggtcttggcg
    56221 agcacctgag tcaatcatat cagatttagt gaccttgtca gcgatacggt tacttgtgag
    56281 ccagtagtcg attctccagc ctgtattgtt gattttagaa gttttgctgc gttgtgccca
    56341 ccaagtgtag cgttcaggaa catcgccatg aacatggcgg aaggtgtctg taaatccagt
    56401 tgccaaaagg ttggtaaatc cagcacgttc ctcgtcagta aatccaggtg aacggcggtt
    56461 gctagcagga tttgcaaggt cgatttcatt gtgggctacg ttgtagtcac cggtcgcaag
    56521 gactggtttt tctttgtcta gttcagccaa atactcagca tatttaacat cccagacttg
    56581 gcgttcttcc aagcgtttga gaccgtcacc agcgtttgga gtgtaaactt gggttacgaa
    56641 aaatgcatca aattctagag tgatgatacg gccttccaag tccatggtag aaggggcacc
    56701 gatttctgga aaactgatcg taggcgtaag ttctttctta taaaggaaca tggttccagc
    56761 atagccttta cgggcaggct cttgggaaga gcgccatgtg ttttcgtagc ctgggaagag
    56821 ttcttctaaa atttccacgt gtttctttgt aggtcctttg gcagaaagct tggtttcttg
    56881 gatagcaatg atatcagcat tttcagcgac caaggtttgt aggacttctt gggacaattt
    56941 ggcacgagct gagtcactag ttagggcagc gtttagggaa tcaatattcc atgagataag
    57001 tttcataaag ttaccttttt cattcagatt atagatttta ttataccaaa aaaagatcta
    57061 tttccccaac gtatggtttg aaaaattact ctctttcgtt tataattaag aatgatttta
    57121 tgaaagggag tgaaaatata tgaaattcta ctcttatgac tatgtactca gccaaatcgg
    57181 tcagcaaaat ggtatcatgg ttggctttgg gattgttcta ttagctgtga cagttttttt
    57241 tgctttcaag gcataccatg ataaaaaggg aaccgaattt cgtgagttgg tcatgatttc
    57301 agctctggcc ttatttagct ctgcttttgg tcagcatcac gacttatcaa aacaatcaag
    57361 tttctaacaa taaatttcaa acttcacttc atttcatcga ggttgtttcc aaagatttgg
    57421 gagtagacaa gtcagaagtc tatgttaata cttccacaaa cacagatggc gcacttatca
    57481 aggtgggaga tcgctattat cgtgccctaa acggaagtga gccagacaag tacctgttag
    57541 agaaagtcga attgtataag acagacgcaa ttgaactggt ggatgtgaac aaatgacact
    57601 taattatatc gaaattttaa tcaaactggt cttgactctc aaatggctca acaacaatgt
    57661 tcactttgtg aaacgtttga ttgatggtaa gccaactctc cttatcaaaa atgggaatat
    57721 tgacccagaa gcctgtcgtt cagttggttt gtctgcatcg gatgtagccc tcaaacttcg
    57781 tagccaaggg attttccaga tgaagcaagt caaacgagct gtgcaagagc aaaatgggca
    57841 acttatcgtt gtgcaaatgg gagatgaaaa tcctaagtat ccagttgtga ctgacggtgt
    57901 gattcaagta gatgtcttgg aatcgattgg tcgtagcgaa gagtggttgc ttgataacct
    57961 cagtaaacaa gggcatgaca atgtagccaa tatctttatt gctgaatatg acaagggtgc
    58021 tgttacagtc gtaacttatg aaaaagaaaa acctggggtc ttgtactctt cgaaaatctc
    58081 ttcaaaccgc gtcaacgtcg ccttgccgta tgtaggttac tgacttcgtc agttctatct
    58141 acaacctcaa agcagtgctt tgagcagcct gcggctagtt tcctagtttg ctctttgatt
    58201 ttcattgagt attggcctca ggtttccatt tgcaatcaga aagggatttt atgtccatta
    58261 ttcaaaaact ttggtggttt ttcaagttag aaaaacgccg ttatctagtc ggaattgtgg
    58321 ccctggtctt ggtttccgtc ctcaatctca ttcctcctat ggttatgggg cgggttattg
    58381 atgccatcac atcggggcaa ttaacccagc aggacctcct tcttagccta ttttacttgc
    58441 tacttgcagc ctttggtatg tactatttgc gctatgtgtg gcgtatgtat atccttggga
    58501 cctcttattg cttgggacag atcatgcggt ctcgcttgtt taagcatttc acaaaaatgt
    58561 cgtcagcctt ttatcaaacc tatcggacgg gtgatctgat ggcacacgca accaatgata
    58621 tcaatgcctt gactcgttta gcaggtggcg gtgtcatgtc tgcggtggat gcctctatca
    58681 cggctctggt gactttgttg accatgctct ttagcatctc atggcagatg actcttgttg
    58741 ccattctccc cctacctttc atggcctatg cgactagtcg cctagggaga aagactcata
    58801 aggcctttgg cgaatcccaa gctgcttttt ctgaactcaa taacaaggta caggagtccg
    58861 tatcaggtat caaagtgacc aagtctttcg gttatcaggc agacgagttg aagtcttttc
    58921 aggcagtcaa tgaattaacc ttccaaaaga acctgcaaac catgaaatat gatagtctct
    58981 ttgaccctat ggttctcttg tttgttggtt cgtcctatgt tttaacgctt ttggttggct
    59041 ccttggtggt tcaggaaggg cagattacag ttgggaatct agtcaccttt atcagctatt
    59101 tggatatgct ggtctggcct cttatggcca tcggcttcct ctttaatact actcagcgag
    59161 ggaaggtttc ttaccagcgg attgaaaatc ttttgtctca ggaatctcct gtacaagacc
    59221 cggagtttcc tctggatggt attgaaaatg ggcgtttgga gtatgccatt gacagctttg
    59281 cttttgaaaa tgaggaaaca ctgacggata ttcactttag tttggcaaaa gggcaaacac
    59341 tgggcttggt tgggcagaca ggctctggga aaacgtcctt aatcaagctc ctcttgcgtg
    59401 aatacgatgt ggataagggc gccatttatc taaacggtca cgatattcgg gactatcgtc
    59461 tgacagacct tcgcagtctc atgggctatg ttcctcagga ccagtttctt tttgcgactt
    59521 caatcctaga caatatccgc tttggcaatc ctaacttgcc cctttcagcg gtcgaggaag
    59581 ctactaagct agcccgagtt taccaagata ttgtagacat gcctcaagga tttgatacgc
    59641 tgattggtga aaaaggagtc agtctttctg gtggtcaaaa gcaacggttg gctatgagtc
    59701 gggctatgat tttagaccct gatatcttga ttttggatga ttccttatcc gccgtagatg
    59761 ccaagacaga gtatgcgatt atcgacaacc tcaaggagac gcgaaaggac aagacaacca
    59821 ttatcactgc ccatcgcctg agtgcagttg tccatgcaga tttgatttta gtcctgcaaa
    59881 atggtcaaat tatcgaacga ggcaggcacg aagacttgct agctttggat ggctggtatg
    59941 cccaaaccta ccagtctcag cagctggaaa tgaagggaga agaagatgca gaataaacaa
    60001 gaacaatgga ctgtattgaa gcgcttgatg tcctatctca agccttatgg actccttacc
    60061 tttttggcac tcagttttct cctagcgacg acggtcatta aaagtgtcat tccccttgtg
    60121 gcttcccact ttatcgacca gtatctcagc aatcttaacc aactagccgt gaccgttttg
    60181 ctggtctact atggtctcta catcctacaa actgtagttc agtatgtcgg caatcttctc
    60241 tttgcgcgcg tgtcttacag tattgttagg gatattcgtc gggatgcctt tgccaatatg
    60301 gagaaactgg gcatgtctta ctttgacaag acgccagcag gttctatcgt ttctcgtttg
    60361 accaacgata ccgagacgat tagtgatatg ttttctggga ttttatccag ctttatctca
    60421 gcagttttta tctttctgac aaccctttat accatgttgg tgctggattt tcgtttgacg
    60481 gctttagtct tgctctttct ccccttgatt ttccttttgg tcaatctcta tcgaaaaaag
    60541 tcagtgaaaa tcatcgagaa aaccagaagt ctcttgtcag atatcaatag taagctggca
    60601 gagaatatcg agggaatcag gattattcag gcttttaatc aagagaagcg cctgcaggca
    60661 gaatttgatg aaatcaacca agagcacttg gcctatgcca accgttctgt agccttggat
    60721 gccctctttt tgagacctgc catgagtttg ttgaaacttc taggttatgc tgtcttgatg
    60781 acctattttg gctatcgtgg tctttctatc gggataacgg ccgggaccat gtatgctttt
    60841 atccagtaca tcaaccgcct ctttgaccct ttgattgagg tgacgcaaaa cttttcaaca
    60901 ctgcaaacgt ctatggtttc tgcaggccgt gtctttgcct taatcgatga gaggacctat
    60961 gagccccttc aagaaaatgg gcaagccaaa gtcaaagaag gcaatatccg ttttgaacat
    61021 gtgtgtttct cgtatgacgg taaacatccg attctggatg acatttcttt ctctgttaat
    61081 aagggtgaaa ccattgcctt tgtaggtcat acaggttcag ggaaatcgtc tattatcaat
    61141 gtcctcatgc gcttttatga attccagtca gggagagttc tcttggatga tgtggatatc
    61201 agggatttca gtcaagaaga gctgagaaaa aacatcggtt tggttttaca ggaacccttc
    61261 ctctatcatg gaactattaa gtccaatatc gccatgtacc aagaaatcag tgatgagcag
    61321 gttcaggctg cggcagcctt tgtggatgca gattccttta ttcaagaact tcctcagggg
    61381 tacgactccc ctgtttccga gcgtggttcg agcttctcta ctgggcagcg ccagcttctt
    61441 gcctttgcta gaacagtcgc cagccagcct aaaatcctga ttttggatga agcgacagcc
    61501 aatattgact ctgaaacaga aagtttggtt caagcttctc tggcgaagat gagacagggc
    61561 cgaacaacta ttgctatcgc tcaccgcctt tctactattc aagacgccaa ctgcatctat
    61621 gtcttggata agggacgcat tatcgagagt ggaacccatg aggaactctt gactctggga
    61681 ggaacctatc acaagatgta tagtttgcag gcaggggcca tggcctatac tctttgaaaa
    61741 tctctttaaa ccatgtcagc tttatctgca atctcaaagc tgtactttga ttttcattga
    61801 gtactagaag gaaatccttc aaattacaga tttctttcac cgccttttcc attttgtggt
    61861 ataatgaaaa atgttgacaa atagtataat aaaaacaaag gagaacagca tgctgaaatg
    61921 ggaagacttg cctgtggaaa tgaaatcaag cgaggttgag tcttactacc agcttgtctc
    61981 taaaaggaag ggttcgctga ttttcaagcg ttgcttggac tgggttttgg ccttggtctt
    62041 actggttcta acttctccca tctttctcat cttgagcatt tggatcaagt tggatagcaa
    62101 ggggccagtg atttacaagc aagagcgtgt gacccagtac aaccgtcggt tcaagatttg
    62161 gaagttccgt accatggtga cggatgcgga taaaaaagga agtctggtga cttctgctaa
    62221 cgatagccgt attaccaagg ttggaaattt catccgacgt gtccgtttgg acgaactgcc
    62281 tcagttggtc aatgtcctta aaggtgagat gtcctttgtc ggtacacgac ctgaagtgcc
    62341 acgttataca gagcagtata gccctgaaat gatggcaacc ttgctcttgc aagcaggaat
    62401 tacctctcca gccagcatca actacaagga tgaggacacc atcatcagtc aaatgacgga
    62461 gaaaggtctg tcagttgatc aggcctatgt ggagcatgtt cttcctgaaa agatgcgcta
    62521 taacctcgcc tatctccgag agtttagttt ctttggggac atcaaaatca tgtttcaaac
    62581 cgtgtttgag gtactaaaat aaagtagtca taagaaaatg agtacagata aaaggagcaa
    62641 atcaatgcca aattacaata ttccattttc accgcctgat atcacagaag cagaaattgc
    62701 tgaagtagcg gataccctgc gttctggttg gatcacaaca ggtcctaaaa caaaagaact
    62761 ggagcgccgc ttgtctcttt acacacagac acctaagact gtttgtctca actctgcgac
    62821 agccgctctg gagttgattt tacgcgtttt ggaagtggga cctggtgatg aagtcatcgt
    62881 tccagccatg acctatacgg cttcatgtag tgtcattacg cacgtgggag caacccctgt
    62941 catggtggat atccaagcag atacgtttga gatggactat gacctgcttg agcaagctat
    63001 cactgagaaa actaaggtga ttatcccagt agagctcgca gggattgttt gcgattatga
    63061 ccgtttgttc caagtcgtgg agaaaaaacg tgacttcttt accgcttcaa gcaagtggca
    63121 aaaggccttt aaccgtattg tcattgtctc tgatagtgcc cacgctttgg gatctactta
    63181 taaaggacaa ccttctggtt ctatcgctga ttttacttcc ttctcattcc atgctgttaa
    63241 gaactttaca acggcagaag gtggaagtgc gacttggaaa gccaatccag tgattgatga
    63301 cgaagagatg tacaaggaat tccaaatcct ttcccttcac gggcaaacta aggatgctct
    63361 tgccaagatg caactggggt catgggaata cgatatcgtt acaccagcct ataagtgcaa
    63421 catgaccgat atcatggctt cacttggttt ggtacaattg gaccgctatc caagtttgtt
    63481 gcaacgccgt aaggacattg tggaccgcta tgatagtggt tttgcaggtt ctcgcatcca
    63541 tcctttggca cacaagactg aaactgtcga atcttcacgc cacctctaca tcacccgtgt
    63601 agaaggagca agcctagaag aacgcaacct catcatccaa gaattggcta aagcaggaat
    63661 tgcaagtaat gttcactaca aaccgcttcc tctcttgaca gcctataaga atcttggatt
    63721 tgatatgacg aactatccta aggcctatgc cttctttgag aatgaaatta ccctccctct
    63781 tcatactaaa ttaagcgatg aagaagtaga ctatatcatt gagactttca aaacagtttc
    63841 tgaaaaagtg ctaactttat caaaaaaatg acaaactaca gtcaagcgaa agtgatcctg
    63901 tccctaaaaa gtctaattga gtgtaaaaac tgttgttttc aattgataat agtttacacc
    63961 tgtagttgag gcctctttct cctcagagag agaattttta taggattttc ctttcttgtg
    64021 ggagtcccgt ggtttgaaat aagatgtgag caatttggta tagcatttag aatccttact
    64081 agacatcatt tagaaaatct agtgtcttgt tctagttttc aattcaccct attttttgaa
    64141 agacgtgagt ttccatgagt gagattgtgg aaactagcgt cttttttttg ttttcagaat
    64201 attgttcaaa gttttgtgcc tgtctttcat gttctagtca ttcttttgca tgatagaatt
    64261 tatagcatgt tgatattata ataacgcaaa tatcccatat gtttagtgat gcttgctata
    64321 catcattaga tctcctgcga gacaatctat aaaacacttg tctacgatta cctatatgcc
    64381 ctattccagt attttagaag cactgcatct atttttatcg aggttaaatc tagcttttat
    64441 agaaggtcta tttaagaaat atattgtagt gttttagttt caatccgcca tatgagcgat
    64501 attcaggtaa atatccctgg cgaatgcttg tatgacaagg tatttgttct ttcatttata
    64561 atttacaaca tatcaacaaa tttaaatata gtaaatggga tattttatat tcaagctaag
    64621 aaagatagca tcacttttga atggaaggct aaagagcaaa ctaggaagtt ggccatagat
    64681 agctcaaaac cctgctttga ggttgtagat atagtaaaat gaaacaagaa taggacaaat
    64741 tgatcgggac agttaaatcg atttctaaca atgttttaga agtagaggtg tactattata
    64801 gtttcagtct actatagaac tgaccaagtc agtaacctag acttagggca aggcggcact
    64861 gacctagttt gaagagattt ccgaagagta taaattttaa tattttcttg tgttattcct
    64921 tgacaattca atttggaaaa tatatgataa agataatgac agcggtgtca ttctatctat
    64981 tttaagaaaa gtaataatca attgttaaaa atagtaaaaa aattggaggt tctgatgaaa
    65041 tattttgttc ctaatgaggt attcagtatt cgtaaattaa aggtggggac ttgctcggta
    65101 ctattggcaa tttcaatttt gggaagccaa ggtattttat cggatgaagt tgttactagt
    65161 tcttcaccga tggctacaaa agagtcttct aatgcaatta ctaatgattt agataattca
    65221 ccaactgtta atcagaatcg ttctgctgaa atgattgcct ctaattcaac cactaatggt
    65281 ttagataatt cgttaagtgt taatagtatc agctctaatg gtactattcg ttccaattca
    65341 caattagaca acagaacagt tgaatctaca gtaacatcta ctaatgaaaa taagagttat
    65401 aaggaagatg ttataagtga cagaattatc aaaaaagaat ttgaagatac tgctttaagt
    65461 gtaaaagatt atggtgcggt aggtgatggg attcatgatg atcgacaagc aattcaagat
    65521 gcaatagatg ctgcagctca agggctaggt ggaggaaatg tatattttcc tgaaggaact
    65581 tatttagtaa aagaaattgt ttttttaaaa agtcatacac acttagaatt gaatgagaaa
    65641 gctacaattc taaatggtat aaatattaag aatcaccctt ccattgtttt tatgacaggt
    65701 ttatttacgg atgatggtgc gcaagtagaa tggggcccaa cagaagatat tagttattct
    65761 ggtggtacga ttgatatgaa cggtgctttg aatgaagaag gaactaaagc aaaaaatcta
    65821 ccacttataa attcttcagg tgcatttgct attgggaatt caaataacgt aactataaaa
    65881 aatgtaacat tcaaggatag ttatcaaggg catgctattc aaattgcagg ttcgaaaaat
    65941 gtattagttg ataattctcg ttttcttggg caagccttac ccaaaacgat gaaggatggg
    66001 caaatcataa gtaaggagag cattcagatt gaaccattaa ctagaaaagg ttttccttat
    66061 gccttgaatg atgatgggaa aaaatctgaa aatgtgacta ttcaaaattc ctattttggc
    66121 aaaagtgata aatctgggga attagtaaca gcaattggca cacactatca aacattgtcg
    66181 acacagaacc cctctaatat taaaatttta aataatcatt ttgataacat gatgtatgca
    66241 ggtgtacgtt ttacaggatt cactgatgta ttaatcaaag gaaatcgctt tgataagaaa
    66301 gttaaaggag agagtgtaca ttatcgagaa agcggagcag ctttagtaaa tgcttatagc
    66361 tataaaaaca ctaaagacct attagattta aataaacagg tggttatcgc cgaaaatata
    66421 tttaatattg ccgatcctaa aacaaaagcg atacgagttg caaaagatag tgcagaatat
    66481 ttaggaaaag tatcagatat tactgtaaca aaaaatgtaa ttaataataa ttctaaggaa
    66541 acagaacaac caaatattga attattacga gttagtgata atttagtagt gtcagagaat
    66601 agtatattcg gtggtaaaga aggaattgtt attgaggatt caaagggtaa aataaccgtt
    66661 ttaaataacc aattttataa tttatccggt aagtatatat cattcatcaa atctaatgca
    66721 aacgggaaag aacctgttat acgtgatagc gatggtaatt tcaatattgt aacggagaat
    66781 gggctttaca aaattgtaac aaataattta agtgataaaa acgaaaaaga aaaaaacaaa
    66841 gaggaaaaac aatctaattc aaataatgta attgatagta accagaagaa cggagagttt
    66901 aactcaagta aagataatag acaaatgaat gacaagatcg acaataaaca agataataag
    66961 acagaagaag taaactataa aatagttgga gatggcagag aaactgaaaa tcatattaat
    67021 aaatctaaag aaatagtaga tgtaaaacaa aaattaccaa agactggttc gaacaagatt
    67081 atggaactat tcttaacagt gacaggaatt ggtttacttt tgacactaaa agggttgaag
    67141 tattatggta aagataaata aaatttgttc gatacaagga agttccgtag aaaatgagga
    67201 tattgtaggt tcacaaaatc agtatttttg gattattggt ggagctacag atttatataa
    67261 ttctaaagag gagataggtt attcagtctc agaagttgta catattttat cagaaagttt
    67321 gtcggttaat tgt
//
LOCUS       DAVKFH010000005        61903 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKFH010000005 DAVKFH010000000
VERSION     DAVKFH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.005"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            147..912
                     /locus_tag="VW006_000303"
                     /pseudo
     CDS             147..912
                     /locus_tag="VW006_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            928..1407
                     /locus_tag="VW006_000304"
     CDS             928..1407
                     /locus_tag="VW006_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379219.1"
                     /translation="MKLNKQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMM
                     MNTSNDLFIKAIELLPFYSLPVFVASNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
     gene            1793..2002
                     /locus_tag="VW006_000305"
     CDS             1793..2002
                     /locus_tag="VW006_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379220.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1992..2480
                     /locus_tag="VW006_000306"
     CDS             1992..2480
                     /locus_tag="VW006_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379221.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            2596..4056
                     /locus_tag="VW006_000307"
     CDS             2596..4056
                     /locus_tag="VW006_000307"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9379222.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4053..4877
                     /gene="nadE"
                     /locus_tag="VW006_000308"
     CDS             4053..4877
                     /gene="nadE"
                     /locus_tag="VW006_000308"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9379223.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5227..5778
                     /locus_tag="VW006_000309"
     CDS             5227..5778
                     /locus_tag="VW006_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379224.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            6493..7185
                     /locus_tag="VW006_000310"
     CDS             6493..7185
                     /locus_tag="VW006_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9379225.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVFVPFATKYEADNDLSAGQEQEITLGKNGKIVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            7239..7628
                     /locus_tag="VW006_000311"
     CDS             7239..7628
                     /locus_tag="VW006_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9379226.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(8289..9317)
                     /gene="queA"
                     /locus_tag="VW006_000312"
     CDS             complement(8289..9317)
                     /gene="queA"
                     /locus_tag="VW006_000312"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9379227.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFY
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            9468..10175
                     /locus_tag="VW006_000313"
     CDS             9468..10175
                     /locus_tag="VW006_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9379228.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            10315..10491
                     /locus_tag="VW006_000314"
     CDS             10315..10491
                     /locus_tag="VW006_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9379229.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            10621..11556
                     /locus_tag="VW006_000315"
     CDS             10621..11556
                     /locus_tag="VW006_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9379230.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            11549..12337
                     /locus_tag="VW006_000316"
     CDS             11549..12337
                     /locus_tag="VW006_000316"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9379231.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEK"
     gene            12338..12721
                     /locus_tag="VW006_000317"
     CDS             12338..12721
                     /locus_tag="VW006_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9379232.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            12737..13126
                     /locus_tag="VW006_000318"
     CDS             12737..13126
                     /locus_tag="VW006_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9379233.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            13210..14340
                     /locus_tag="VW006_000319"
     CDS             13210..14340
                     /locus_tag="VW006_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9379234.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            14345..15082
                     /locus_tag="VW006_000320"
     CDS             14345..15082
                     /locus_tag="VW006_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9379235.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            15093..15866
                     /locus_tag="VW006_000321"
     CDS             15093..15866
                     /locus_tag="VW006_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9379236.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            15856..16473
                     /locus_tag="VW006_000322"
     CDS             15856..16473
                     /locus_tag="VW006_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9379237.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            16683..17084
                     /locus_tag="VW006_000323"
     CDS             16683..17084
                     /locus_tag="VW006_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9379238.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            17084..17362
                     /locus_tag="VW006_000324"
     CDS             17084..17362
                     /locus_tag="VW006_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9379239.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            17352..18125
                     /locus_tag="VW006_000325"
     CDS             17352..18125
                     /locus_tag="VW006_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9379240.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            18126..19430
                     /locus_tag="VW006_000326"
     CDS             18126..19430
                     /locus_tag="VW006_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9379241.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            20004..20882
                     /gene="pstS"
                     /locus_tag="VW006_000327"
     CDS             20004..20882
                     /gene="pstS"
                     /locus_tag="VW006_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9379242.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            20882..21799
                     /gene="pstC"
                     /locus_tag="VW006_000328"
     CDS             20882..21799
                     /gene="pstC"
                     /locus_tag="VW006_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9379243.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            21789..22673
                     /gene="pstA"
                     /locus_tag="VW006_000329"
     CDS             21789..22673
                     /gene="pstA"
                     /locus_tag="VW006_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9379244.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            22684..23487
                     /gene="pstB"
                     /locus_tag="VW006_000330"
     CDS             22684..23487
                     /gene="pstB"
                     /locus_tag="VW006_000330"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9379245.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            23500..24258
                     /gene="pstB"
                     /locus_tag="VW006_000331"
     CDS             23500..24258
                     /gene="pstB"
                     /locus_tag="VW006_000331"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9379246.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            24270..24923
                     /gene="phoU"
                     /locus_tag="VW006_000332"
     CDS             24270..24923
                     /gene="phoU"
                     /locus_tag="VW006_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9379247.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            25080..25895
                     /locus_tag="VW006_000333"
     CDS             25080..25895
                     /locus_tag="VW006_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9379248.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(25915..27186)
                     /locus_tag="VW006_000334"
     CDS             complement(25915..27186)
                     /locus_tag="VW006_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9379249.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            27328..28059
                     /gene="budA"
                     /locus_tag="VW006_000335"
     CDS             27328..28059
                     /gene="budA"
                     /locus_tag="VW006_000335"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9379250.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            28069..28821
                     /locus_tag="VW006_000336"
     CDS             28069..28821
                     /locus_tag="VW006_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9379251.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            28933..29838
                     /gene="murB"
                     /locus_tag="VW006_000337"
     CDS             28933..29838
                     /gene="murB"
                     /locus_tag="VW006_000337"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9379252.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            29998..31155
                     /locus_tag="VW006_000338"
     CDS             29998..31155
                     /locus_tag="VW006_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379253.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            31136..31942
                     /locus_tag="VW006_000339"
     CDS             31136..31942
                     /locus_tag="VW006_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379254.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            31939..32712
                     /locus_tag="VW006_000340"
     CDS             31939..32712
                     /locus_tag="VW006_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379255.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            32709..33779
                     /locus_tag="VW006_000341"
     CDS             32709..33779
                     /locus_tag="VW006_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9379256.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            34040..34279
                     /locus_tag="VW006_000342"
     CDS             34040..34279
                     /locus_tag="VW006_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379257.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            34554..35039
                     /locus_tag="VW006_000343"
     CDS             34554..35039
                     /locus_tag="VW006_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9379258.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            35061..37679
                     /gene="alaS"
                     /locus_tag="VW006_000344"
     CDS             35061..37679
                     /gene="alaS"
                     /locus_tag="VW006_000344"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9379259.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPCITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(37769..39223)
                     /locus_tag="VW006_000345"
     CDS             complement(37769..39223)
                     /locus_tag="VW006_000345"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9379260.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            39402..40151
                     /locus_tag="VW006_000346"
     CDS             39402..40151
                     /locus_tag="VW006_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379261.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            40132..41766
                     /locus_tag="VW006_000347"
     CDS             40132..41766
                     /locus_tag="VW006_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379262.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            42451..43614
                     /locus_tag="VW006_000348"
     CDS             42451..43614
                     /locus_tag="VW006_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9379263.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            43611..44288
                     /locus_tag="VW006_000349"
     CDS             43611..44288
                     /locus_tag="VW006_000349"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9379264.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            44278..45132
                     /locus_tag="VW006_000350"
     CDS             44278..45132
                     /locus_tag="VW006_000350"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9379265.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALENVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            45151..46218
                     /locus_tag="VW006_000351"
     CDS             45151..46218
                     /locus_tag="VW006_000351"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9379266.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKTRGNTLKLVLVS
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            46228..47394
                     /gene="aroC"
                     /locus_tag="VW006_000352"
     CDS             46228..47394
                     /gene="aroC"
                     /locus_tag="VW006_000352"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9379267.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            47404..48507
                     /locus_tag="VW006_000353"
     CDS             47404..48507
                     /locus_tag="VW006_000353"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9379268.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            48518..48856
                     /locus_tag="VW006_000354"
     CDS             48518..48856
                     /locus_tag="VW006_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9379269.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            48948..50231
                     /gene="aroA"
                     /locus_tag="VW006_000355"
     CDS             48948..50231
                     /gene="aroA"
                     /locus_tag="VW006_000355"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9379270.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            50224..50700
                     /locus_tag="VW006_000356"
     CDS             50224..50700
                     /locus_tag="VW006_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9379271.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            50697..51545
                     /gene="pheA"
                     /locus_tag="VW006_000357"
     CDS             50697..51545
                     /gene="pheA"
                     /locus_tag="VW006_000357"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9379272.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            51542..52816
                     /gene="psr"
                     /locus_tag="VW006_000358"
     CDS             51542..52816
                     /gene="psr"
                     /locus_tag="VW006_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9379273.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            53095..53940
                     /locus_tag="VW006_000359"
     CDS             53095..53940
                     /locus_tag="VW006_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9379274.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            53930..55033
                     /locus_tag="VW006_000360"
     CDS             53930..55033
                     /locus_tag="VW006_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9379275.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            55035..56021
                     /locus_tag="VW006_000361"
     CDS             55035..56021
                     /locus_tag="VW006_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9379276.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            56037..57470
                     /locus_tag="VW006_000362"
     CDS             56037..57470
                     /locus_tag="VW006_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9379277.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            57499..58533
                     /locus_tag="VW006_000363"
     CDS             57499..58533
                     /locus_tag="VW006_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9379278.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            58733..59470
                     /gene="mecA"
                     /locus_tag="VW006_000364"
     CDS             58733..59470
                     /gene="mecA"
                     /locus_tag="VW006_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9379279.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            59621..60907
                     /locus_tag="VW006_000365"
     CDS             59621..60907
                     /locus_tag="VW006_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9379280.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            60909..61778
                     /locus_tag="VW006_000366"
     CDS             60909..61778
                     /locus_tag="VW006_000366"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9379281.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
BASE COUNT        18044 a        11414 c        14025 g        18420 t
ORIGIN      
        1 gcgcaacttt ttaaactcaa taagaatcaa agagcaaacc atgaatatta atggaggaat
       61 aaaaaatgta agttataaat gtaagtaagc attatggtca ttcaatcatt ctcaaagata
      121 taaattttgc acttaacaag ggtgaaattg ttggtctagt agggagaaat ggagttggta
      181 agagtacgtt gatgaaaatt cttgttcaaa ataatcaacc gacttcaggt aatattataa
      241 acagtgataa tgttgggtat ttaatcgaag aaccaaaatt atttttatct aaaacaggtt
      301 tagagaattt aaaatatttg tcaaatttat atggtgttga ctacaatcaa gaaagattta
      361 gaagtttgat ccaagagtta gatttgactc agtctattaa taaaaaagta aagacctatt
      421 ctttgggtac aaaacaaaaa ttagctttgc ttctaactct cgttacggaa cctgatatat
      481 tgattttaga tgaaccgact aatggtttag atattgaatc atcacaaata gttttagcgg
      541 ttctaaaaaa aattagcttt aaatgaaaac gtgggaattt taatatcgag tcataaatta
      601 gaagacattg aagaaatttg tgagagggtt cttttcttgg agagcgggct tttgacattt
      661 caaaaagtag gaaaagatag tcataatttc ttgtttgaga tagctttttc atcagctaca
      721 gatagagaca ttttcattac caaacaagaa tttggggata ttgttcagga agagggattg
      781 agaattacta tgtctgggaa tattcaaaat agtgagcttt ttaaattttt taacgaaaac
      841 tctattaaag tagttgattt tgaaactaaa aaagagacgc ttaaagatat ttacctaaat
      901 cgttcaaaat aaaggaaggt tataatcatg aaattaaata aacagaagaa tcggatgatt
      961 tacgtcttgt ctaattttct atatgccatc tcagtttcca ttatttatgc tttgaatggc
     1021 attgtgttac tagtcatagt aagtaaattg ggtattccag gtgatttagg attagatttt
     1081 atagtagcta ttgtagtcaa tacaattttg ttagtcctgt tttattttct attatcttac
     1141 attttttatt tatacaaatt gaaaagtggc ttggtatttg gtattttagt agctttacta
     1201 ctctttatcc ctaatatatt aaatacgatg atgatgaata ctagtaatga tttgtttatc
     1261 aaagcaattg aacttcttcc tttttattct ttacctgtat ttgtggcttc aaatacgatg
     1321 tctattagtc agtatcttgt ggtaatcact acaatcattt tattgtactt tttcactctc
     1381 aagaaaagca agaagtattc attttagttt catttagtat tattttgcat acctaaaata
     1441 caataaaaaa atcagtcatc ttggtatgct cctgctttca ctattcaaca cgtttttgac
     1501 ttatactagg ctcatttcca aaagcattat ataatagtga tatgaaacca actaaactaa
     1561 acaagaaata taagcaataa aaactcgttt aaaagatcct actaaagcta atactaaata
     1621 aaaataaaag agtaaactag gaagtttatt tcaaacaacc taaaatactg attttcggct
     1681 gaagataata ctggagtgca aattaatggg gttataataa atagctgata gcttgtgttg
     1741 gttttggatt ttttaagagt agatgagtat taaaactata aggaggacga aggtggctaa
     1801 aaatttaaaa ttaaaattag ctcgggtaga gcgtgattta acacaaggtc aactggcaga
     1861 ggctgtcggg gtgacgcgcc agactattgg tttgatagaa gcggggaaat acaatcccag
     1921 tctctcgctc tgccagtcca tttgcagatg tttagggaaa accctagacc aactattttg
     1981 ggaggaagaa gatgaaaaat agattttatt attatcaatt actagacgaa agagaagaac
     2041 aactgttcaa taaagcgggc tctgaaagtt tctatatctg catcgctttg tcgctcctat
     2101 cttatatcat ttcagtatta gcaccaagcc tttttaattc taatatgctg ctaatcgtta
     2161 tcatcatagg gacattttac tttttcaatc gtgcccgtta tctgggagtg acctactata
     2221 gtcgttttca ttttacgatt ttgggttgtt ttttcctaac cttggctatt acggctcttt
     2281 tgatgttgca gaattatcaa ttcaacatag aaatttatca gcacaatcct ttgaatttta
     2341 aatacctgtc tgcttgggtc attacttata tcatttacct tccgtggatc tttattggca
     2401 atcttggtct taagagctat ggcgaatggg ctcagaaaaa atttgaacaa gatatggatg
     2461 aattggagag tggagaatag cttgttactc ttttctcaat ccagctaaaa tgtgatataa
     2521 tagtactaat ttattggaat acatgaaagt tcttgaaaat tttcatgggt ttctagctaa
     2581 ggaagtagga aaagtatgta tccagatgat agtttgacat tgcacacgga cttgtaccag
     2641 atcaacatga tgcaggttta ctttgaccaa gggattcaca ataagaaggc ggtctttgag
     2701 gtgtatttcc gccaacagcc ttttaagaac ggctatgcgg tttttgcagg tttggaaaga
     2761 attgtgaact atcttgaaga cttgcgtttt tcagatagtg atatagccta tttggagtcg
     2821 cttggttatc atggggcgtt cttggattac cttcgcaatt tcaagttgga gttgaccgtt
     2881 cgttctgccc aagaagggga tttggttttt gctaatgaac cgattgtgca ggtggaagga
     2941 cctctagccc aatgtcagtt ggtcgaaacg gctcttttga acatcgtcaa ctaccagacc
     3001 ttggtggcga cgaaggcagc tcgtattcgt tcggttatcg aagatgaacc cttgatggag
     3061 tttgggacac gtcgggctca agaaatggat gcggccatct ggggaacacg cgcagctgtg
     3121 attggtggcg ccaatggaac tagcaacgtg cgtgcgggta agctctttga cattcctgtt
     3181 ttgggaaccc atgcccatgc cttggtacag gtttatggca atgactatga agctttcaag
     3241 gcttacgctg cgacccacaa aaattgtgtc tttcttgtgg atacctatga cacccttcgc
     3301 atcggtgtac cagctgccat tcaggtggcg cgtgagctgg gtgatcagat taactttatg
     3361 ggtgtgcgga ttgactctgg ggatattgcc tacatttcta agaaagtccg tcagcaactg
     3421 gacgaggctg gatttacaga ggctaagatt tatgcttcta atgatttgga cgaaaatact
     3481 atcctcaatc tcaagatgca aaaggccaag attgatgtct ggggcgtggg taccaagctg
     3541 attacagcct atgaccagcc agctcttggt gcagtttaca agattgttgc aatcgaagat
     3601 gaaactggtc agatgcgcaa tacgattaag ctgtctaata atgctgaaaa agtttctacg
     3661 ccaggtaaga agcaggtgtg gcgcattacc agtcgtgaaa aaggcaagtc agaaggcgac
     3721 tatatcactt atgatggtgt ggatattagt gacatgacag aaatcaagat gttccatccg
     3781 acctatacat acatcaagaa gacggttcgt aattttgatg ccgttcctct cttggtggat
     3841 atcttcaaag aaggaatatt agtttacaac ttgcctagtt tgactgacat tcaggattat
     3901 gcccgtaaag aatttgacaa gttgtgggat gagtataagc gtgtgctcaa tccgcagcac
     3961 tatccagtgg atttggcgcg tgatgtatgg caagataaga tggacttgat tgataagatg
     4021 cgcaaggaag cccttggtga aggagaagaa gaatgagttt gcaagaaacg attatccaag
     4081 agctgggtgt caaaccagtg attgatgccc aggaagaaat ccgtcgttct attgatttct
     4141 taaaaagata tctgaaaaaa catcccttcc taaaaacctt tgtactaggg atttctgggg
     4201 gacaagactc aaccttggca gggcgtttgg cgcaattagc tatggaagaa ctgcgagctg
     4261 aaacgggaga cgatagctac aaatttatcg ctgtccgcct gccatacgga gtgcaagctg
     4321 atgaagcaga tgctcaaaaa gctctagcct tcatccagcc agatgtcagc ttggttgtga
     4381 atatcaagga atcagctgat gccatgacag ctgcagttga agcgacaggt agtcctgttt
     4441 cagacttcaa caaggggaat atcaaggcac gttgccgtat gattgctcag tatgcccttg
     4501 ctggttccca tagcggagcg gtcattggaa cagaccacgc cgcggaaaat atcacaggtt
     4561 tctttaccaa gtttggtgac ggcggtgcgg atattctccc tctttaccgc ctcaataaac
     4621 gccaaggaaa acagctcttg cagaaacttg gcgcagagcc agccctttat gaaaaaatcc
     4681 caacggcaga cctagaagaa gataaaccag gcctagctga cgaagtcgca cttggagtca
     4741 cctacgcaga gattgacgac tacctagaag gcaaaacaat cagcccagaa gctcaagcga
     4801 ccattgaaaa ctggtggcac aaaggccaac acaaacgcca cttacccatc actgtatttg
     4861 atgacttttg ggagtaaaaa ggtccggggg acctttttag cttcttgccc tgaaattaaa
     4921 aagcaagaaa aacctccact ggaggttttc agcctctcat cttaaaataa gaaagtgaga
     4981 gaaggtctgg gggatcttga accccgagtt tagaaataag aaaatgaggc agattcggta
     5041 actcgaagag ttcgatttca tcgtcttacc cctgcaacga tgactaggtt tgaaaaagct
     5101 tgctagatcg catttcaaac caggcagcga ttgcgtcaag aaattagaag acaaactcgt
     5161 tttctagctg ttactgagtt gagccttttt actacgagca tagaaataag gaagtgaggt
     5221 agcatcatga aatctattgg tacgcaaata ttacagacag aacgtttgat tttaagaaga
     5281 tttgtggaga gtgatgcaga agctatgttt caaaattggg cttcatccgc tgagaatctg
     5341 acctatgtta cctgggatcc ccatcctgat atcgaaatca ctcgaaactc gattcgcaat
     5401 tgggttgctt cctatactaa tctcaactat tataaatggg ccatttgtct aaaagaaaac
     5461 ccagagcaag tgataggaga tatcagcatt gttaagatag acgaggctga tttaagctgt
     5521 gaaatcggct atgtgctagg caaggcttac tggggacatg gtatgatgac agaggccttg
     5581 aaagctatct tggacttttg ttttactcaa gcaggttttc aaaaggtcag agcacgttat
     5641 gccagtctca acccagcttc aggtcgtgtc atggaaaagg ctggaatgtc ctatctacaa
     5701 accattgtta atggtgtaga gagaaaaggc tatcttgcgg atcttattta ttatggtata
     5761 agtagggaag aatgttgaat tctattttct gtttctatcg aagtcaacta tttattgtaa
     5821 atataataat tagcattcca agtttatttg aaactttaaa atagcatatt gattagtaca
     5881 agacagatgt tctagttcct tctttaatct ggtttagtat tagttaaaaa atcgctttaa
     5941 gcttgtaact aagagggacc taatcgacta gattctccag ccgaacaggt ggtaatgtac
     6001 tttttatagt gtaatcctag ctgttgttaa atttaaaata gaatcctcta tcgagttagg
     6061 gaattaaatt caaccaattt tattcatgtt ttttctatca aattatctaa tattaaaata
     6121 gtctcattct gatgagaaaa ctattctatt tatactgcat agttgtataa ggaatgatga
     6181 aagatgagtg aaaattatca agttggaatg tttgtatcta aatatattag catgtattta
     6241 gataagatgt ctgcaatcct ttatatatga gattgattac gatatagtta aagtgttagt
     6301 tcaataatca atatatcatt ctaagtctat tttatatata aagtatattg tttaacgttt
     6361 gatagaaaag atgctatact agaagtagga tgtaatggca aaatctaata ctaataagcg
     6421 ctatgcaata cgtaaactcg gaaagatttt tggttctgcg gtaatcgggc taactttatt
     6481 tttaggagga atctgaatac aagttttgtt catgctgctg atgggattca atatgtcaga
     6541 gatgatacta gagataaaga agagggaata gagtatgatg acgctgacaa tggggatatt
     6601 attgtaaaag tagcgactaa acctaaggta gtaaccaaga aaatttcaag tacgcgaatt
     6661 cgttatgaaa aagatgaaac aaaagaccgt agtgaaaatc ctgttacaat tgatggagag
     6721 gatggctatg taactacgac aaggacctac gatgttaatc cagagactgg tcatgttacc
     6781 gaacaggtta ctgttgatag aaaagaagcc acggatacag ttatcaaagt tccagctaaa
     6841 agcaaggttg aagaagtttt tgttccattt gctactaaat atgaagcaga caatgacctt
     6901 tctgcaggac aggagcaaga gattactcta ggaaagaatg ggaaaatagt tacaacgata
     6961 acttatgatg tagatggaaa gagtggacaa gtaactgaga gtactttaag tcaaaaagaa
     7021 gactctcaaa caagagttgt taaaaaagga accaagcccc aagttcttgt ccaagaaatt
     7081 tcaatcgaaa cagaatatct cgatggccca actcttgata aaagtcaaga agtagaagaa
     7141 gtaggagaaa ttggtaaatt actcttacta caatctatac tgtagatgaa cgtgatggaa
     7201 caattgaaga aactacttct cgtcaaatta ctaaagagat ggtaaaaaga cgtataagga
     7261 gagggacgag agaacctgaa aaagttgttg ttcctgagca atcatctatt ccttcgtatc
     7321 ctgtatctgt tacatctaac caaggaacag atgtagcagt agaaccagct aaagcagttg
     7381 ctccaacaac aggctggaaa caagaaaatg gtatgtggta tttttataat actgatggtt
     7441 ccatggcaac aggttgggta caagttaatg gttcatggta ctacctcaac agcaacggtt
     7501 ctatgaaagt caatcaatgg ttccaagttg gtggtaaatg gtattatgta aatacatcgg
     7561 gtgagttagc ggtcaataca agtatagatg gctatagagt caatgataat ggtgaatggg
     7621 tgcgttaaca gattcctccc tgcgacaaaa ttgataatag tatgtctgaa tatataatta
     7681 tatataaaaa gctatagata aggtcatctt atagcgacta taaaaaagtg aacaagccta
     7741 gtcttctgta cattagaatt cagatttgtt cactttttgt ttaatactca atgaaaatca
     7801 aagagcaaac tagaaaacta gtcgcaggtt gctcaaagta ctgctttgag gttgtagata
     7861 gaactgacga agtcagtcac atatataatc caaggtgacg ttgacgcggt ttgaagagat
     7921 ttttgaagag tataagtctt tgattttgag actaaggaga gtcagggtca aagtagtgaa
     7981 tgattctgaa ataactcata atagtctctt gagaggacag ataagagtaa caaatatggt
     8041 gctactcatg tgtcttgcat tttgatttac aaacagcgaa gaaaatatag gaagctgaaa
     8101 taaaatatag atgaatcaat ttggagagtc aacgcaatct atggcaatat tttcgaagct
     8161 agggggaagc attttaattt caatgtacta ggcgtcttct acagctagct agagtttcaa
     8221 ttgtagatgt tctttgaaat aatatcttag cgatttattg gtattagaag aattagagaa
     8281 attctttctc aataaataaa catggcgtca ccaaaactga agaagcggta gtgttcttgg
     8341 atggcatggt ggtaggcatc taagactaat tcacggcctg caaaggcaga aaccaacatg
     8401 accagagttg attttggcag atggaagttg gttgagaagg catccacgac cttccactca
     8461 tacccaggtt tgataaagat attggtccaa ccagaatctg cttggatttg cccatcaaac
     8521 ttggaaccaa tagtttccaa ggtgcggata gaagtggttc cgacagcgat gacgcgacca
     8581 ccattttctt tgacagagcg aagggtggca gcagcttcct cagaaagttg atagaactct
     8641 gagtgcattt cgtgttcgtc cagattatcc acagaaacag gtctaaaggt tccgagtccg
     8701 acatggagag tcagatagac tagatgaaca cccttagctt ggatttctgc cagcagttct
     8761 ttggtgaagt gaagaccagc agtcggtgct gcagcagagc cgctttcctt ggcgtagacg
     8821 gtttgataac gttcacggtc atctaatttt tcgtggatat aaggtggcag aggcatttct
     8881 cccagacttt ccaagacttc taggaaaatt ccttggtatt caaagcggac aatgcgtccc
     8941 ccgtgggtca attcttctgt aacgacagcg ctgaggcggc catcaccaaa gctgatacga
     9001 gtaccgacct tgaggcgttt ggcaggttta gccagaactt cccactcgtc tccactagtg
     9061 ttcttaagga ggagaagttc cacatgacct cctgtctcca ctttttgacc atagaggcgg
     9121 gcagggagaa ctcgggtgtc gttcatgaca agggcatcac caggttccag catatcaata
     9181 atagagtaga agtgtttatc ttgcatttct cctgtctcac ggttgacgat gaggagtttg
     9241 gaggcatcac gtttttcaag gggcgtttgg gcaatcaatt cctctggcaa gtggaaatca
     9301 aaatcagctg tattcatata tttgttcatt ctttctaagt tctaatccta tccattataa
     9361 cacgtttcaa gtttggacgc tcttttttta gaaattaaat cgttttcact tgacaaaaat
     9421 tggtctatac catataataa atatagacag aaatggagga tgaaaagatg aaagttatta
     9481 aagttgaaaa ccaagttcaa ggtggaaaag ttgcttttga gattttgaag gaaaaattgg
     9541 ctaatggagc tcaaactcta ggacttgcga caggaagtag cccacttgaa ttttacaagg
     9601 aaattgttga gagtgacctt gatttttcaa atctaaccag tgtcaacctt gatgagtatg
     9661 tgggccttga tggggacaat ccacaatctt atcgttactt catgcaagaa aacttgttca
     9721 accaaaaacc atttaaagaa agtttcttgc ctcgtggtgt taaggacaat gctgaagctg
     9781 aagttgaacg ctacaaccaa attttggctg accatccagt tgacctccaa atcttgggaa
     9841 tcggtcgcaa tgggcatatc ggctttaatg agcctggtac tccatttgac agtcaaacgc
     9901 atctagtaga acttgaccag tctactatcg aagccaatgc acgcttcttt gccaagattg
     9961 aagacgtccc aacccaagct atttcaatgg ggattaaaaa cattttggat gccaagtcta
    10021 ttattctttt tgcttacggt gagtcgaaag cagaagccat tgccggaaca gtatctggcc
    10081 cagtgactga gaacctacct gcaagtagcc ttcaaaatca ccctgatgta accatcatcg
    10141 cagatgctga agcgcttagc ttactcgaaa aataaaaaag caatgttcta ttgaacgctt
    10201 tcaactcttg tataaatgga agaaaaaatc aaaattaaac cgcatttttg cttgacaatt
    10261 attcctttta cgtgtagaat gaaatagata ttgaacttga agggagtgaa aaaaatgtct
    10321 aaaacagtag tacgtaagaa tgaatctctt gacgacgcac ttcgccgttt caaacgtgcg
    10381 gttactaaag ctggtactct tcaagaaaca cgcaaacgtg aattctatga aaaaccttct
    10441 gtaaaacgta aacgtaaatc agaagtagct cgtaaacgta aaaaattcta attgaaatga
    10501 aaggctagag aaatctagtc ctttttcttt taaataaata ctccaaagcc tgcaaaaatc
    10561 tgaaacttcc tcctacaatt tgatataata gagagaagaa ctcatttgaa ggaggaaatg
    10621 atgtcggttt tagtaaaaga agtgattgaa aagcttagac tagacattgt ctatggtgaa
    10681 ccagagttgc ttgaaaagga aatcaataca gcggatatta cgcgacctgg tcttgaaatg
    10741 acaggctatt ttgactacta tacaccagag cggattcaac ttttggggat gaaggagtgg
    10801 tcttatctga tcagcatgcc ttccaacagc cgttatgaag ttttgaaaaa aatgtttcta
    10861 cctgagacac cagcagtcat tgttgctcgt ggtttggtgg ttccagagga gatgttaaag
    10921 gctgctagag aatgtaagat tgctatttta accagccgtg cagctaccag tcgtttatct
    10981 ggagagttat ctagctatct ggattctcgt ttggcagaac gtaccagtgt gcacggtgtc
    11041 ttgatggata tttatgggat gggcgtcttg attcagggag atagtggaat tggtaagagc
    11101 gagacaggtc ttgagcttgt caaacgtggt caccgtttgg tagccgatga ccgtgtcgat
    11161 atctttgcca aggatgagat tactctctgg ggtgaaccag ctgaaatttt gaaacacttg
    11221 attgaaattc gtggggttgg tattatcgat gttatgagtc tctacggtgc gagtgctgtc
    11281 aaggattctt cacaggttca gcttgctgtc tatttggaaa attacgatac acataagacc
    11341 tttgatcgtc ttggaaacaa tgcagaggaa cttgaagttt ctggcgtagc cattcctcgt
    11401 attcgtattc cagttaaaac aggtcgtaat atctctgttg tgattgaggc agctgccatg
    11461 aattatcgtg ccaaggaaat gggctttgat gcgacccgtt tgttcgacga acgactgaca
    11521 agtctcattg ctcgaaatga ggtgcaaaat gcttgatcca attgctattc aactaggacc
    11581 cctagccatt cgttggtatg ccttatgtat tgtgacaggc ttgattcttg cggtttattt
    11641 gaccatgaaa gaagcaccta gaaagaagat cataccagac gatattttag attttatctt
    11701 agtagccttt cccttggcta ttttaggagc tcgtctctac tatgttattt tccgatttga
    11761 ttactatagt cagaatttag gagagatttt tgccatttgg aatggtggtt tggccattta
    11821 cggtggtttg ataactgggg ctcttgtgct ctatatcttt gctgaccgta aactcatcaa
    11881 tacttgggat tttctagata ttgcggcgcc tagcgttatg attgctcaaa gtttggggcg
    11941 ttggggtaat ttctttaacc aagaagctta tggtgcaaca gtggataatc tggattatct
    12001 acctggcttt atccgtgacc agatgtatat tgaggggagc taccgtcaac cgactttcct
    12061 ttatgagtct ctatggaatc tgcttggctt tgctttgatt ctgattttta gacggaaatg
    12121 gaagagtctc agacgaggtc atatcacggc cttttacttg atttggtatg gtttcggtcg
    12181 tatggtcatc gaaggtatgc gaacagatag tctcatgttc ttcagccttc gagtgtccca
    12241 atggctgtca gttgtcctta tcggtctcgg tataatgatc gttatttatc aaaatcgaaa
    12301 gaaggcccct tactatatta cagaggagga aaaataaatg ttagaagttg catatattct
    12361 tgttgcccta gctttgattg tctttttggt ctatctgatc attactgtac aaaagcttgg
    12421 tcgtgtcatc gatgaaacag aaaagacgat taaaaccttg acttcagatg tggatgtgac
    12481 cttgcatcac accaatgagt tgttggctaa ggtcaatgtc ttggcagatg atatcaatgt
    12541 caaggtggct acgattgatc cactcttcag tgctgttgca gatttatctc tatctgtttc
    12601 agacctcaat gaccatgcgc gtgtcttgag caagaaagct tcatcagctg gttcaaaaac
    12661 actcaagact ggtgcaagtc tgtcagctct tcgtcttgca agtaaatttt tcaaaaaata
    12721 aaaaaggaga atccttatgg gtaaattatc ctcaatcctt ttaggaacgg tttcaggtgc
    12781 agctcttgcc ttgtttttaa caagtgataa gggcaaacaa gtttgcagtc aggctcaaga
    12841 ttttctagat gatttgagag aagatccgga gtatgccaag gagcaagtct gtgaaaaact
    12901 gacagaagtt aaggagcagg ctacagattt tgttctgaaa acaaaagaac aggttgagtc
    12961 aggtgaaatc actgtggaca gtatacttgc tcaagctaaa tcctatgctt ttcaagcgac
    13021 agaagcatca aaaaatcaat taaataatct caaggaacaa tggcaagaaa aagccgaagc
    13081 tcttgatgac tcagaagaga ttgtgattga tataacagaa gaataaacca tcaccatctc
    13141 cggacggact atgtatctgg ggatggtgat ttttatctgg aatctagtct ttgtggtata
    13201 ataattacta tgcagaaaaa accaacgtca gcctatgtgc acatcccatt ttgtacccag
    13261 atttgttatt attgtgattt ttcaaaggtc ttcatcaaaa atcagccagt cgacagctat
    13321 ttagagcatc tgctggaaga gtttcgttct tatgatattg aaaagttgtc aaccctttat
    13381 atcggtggtg gaacaccgac agccctgtcg gctccgcaac tggaggtgtt actgaatggc
    13441 ttgactaaaa acttggactt gtctgtcttg gaagagttga ccattgaagc caatccaggc
    13501 gatttggatg cggataagat agctgttttg aaaaattcgg ctgtcaatcg tgtttcgcta
    13561 ggtgtccaga cctttgatga taagatgttg aaaaagattg ggcgcagtca tttggagaag
    13621 gatatttatg aaaatatcga tcgtctgaaa ctggctggtt ttgacaacat ctccattgat
    13681 ttgatttatg ctctgcctgg tcagaccatg gagcaagtaa aggaaaatgt ggctaaagcc
    13741 attggactgg atattcccca catgagtttg tatagtttga ttttagaaaa ccatacggtc
    13801 tttatgaacc ggatgcgacg tgggaaattg cctctgccta aggaggaact agaagcggag
    13861 atgtttgagt acatcattgc agagctggag cgagcgggtt ttgagcatta tgagatttct
    13921 aatttctcca aacccggttt tgaaagtcgt cacaatctca tgtactggga caatgctgaa
    13981 tactatggta ttggtgcagg ggcatctggt tatgtcaatg gagtacgcta taaaaatcat
    14041 ggtcccattc gtcattatct cagtgcggtt gaggaaggca atgcttgtat tacagaagat
    14101 cacctgagtc aaaaggagca aatggaagaa gaaatgttct tgggactccg caagaaatcc
    14161 ggggtttcca tggcgcgatt tgaggaaaaa tttggacagt cttttgctgg actttatgga
    14221 gaaattgtca gagatttggt tcaacaaggc ctcatgcaga ttgagggtga ccacgttcgc
    14281 atgacaaaga gaggtctctt tttgggagat actgtagcag aacgatttat tttggagtag
    14341 gatcatgggc ttaacttatc aaatgaaaat gaaaattcct tttgatatgg ctgatatgaa
    14401 cggtcatatc aaacttccag atgtgatttt gctgtcactg caagtttcag ggatgcagtc
    14461 gattgaactg ggagttagtg ataaggccat tttggaagaa tataatctgg tctggattat
    14521 cgtagaatat gatattgagg tggttcgttt gcctcgtttt gcggaagaaa ttaccatcga
    14581 aacggaagcc ttgagctaca atcgactttt ttgctaccgt cgctttacta tttataatga
    14641 agcggagcag gagctcatcc acatgatggc gacctttgtt ctcatggacc gcgacagtcg
    14701 aaaagtccat gttgttgaac ctgagattgt ggctccttac cagtctgatt ttgataaaaa
    14761 acttatccgt ggaccgaagt atgagtcctt ggaagaaccg atcagcaagg attaccatgt
    14821 tcgtttttac gacttggata tgaatggtca tgtcaataac agtaaatact tggactggat
    14881 ttttgaggtc atgggagcgg attttttgac ccactatatt cccaagaaaa tcaacctcaa
    14941 gtatgtcaag gaagttcgac caggtggggt gattacatcg gctgttgaac ggactggact
    15001 ggaaagcaag catgaaatta caagtgatgg ggttatcaat gcccaagcaa tcattacttg
    15061 gcaagaaata aaaaaggctt agggagaaat agatgaaata taaaggctat ttaattgatt
    15121 tagacggaac catttataag gggaaagacc gaatccctgc tggagagact tttgttcatg
    15181 aattgcaaaa gcgggacatt ccctatctct ttgtaaccaa caatacaacc cgcactccag
    15241 agagtgttaa ggagatgttg gctcagaatt ttaatatcga tacgccccta tcgactgtct
    15301 acacagcgac tttggcaacc atcgactata tgaatgattt ggggcttgaa aagaccgtct
    15361 atgtcgtcgg agaagcagga ctcaaggaag ccatcaaggc ggctggttat gtggaagaca
    15421 aggaaaaacc tgcctacgtg gtagttggtc tggattggca agtcgactat gaaaaatttg
    15481 ccacagcaac tcttgctatt caaaagggtg cccattttat cggaaccaat cctgacctca
    15541 acatcccgac ggaacgcggt cttttgccag gtgctggctc actgattacg ctgcttgaag
    15601 tagcgacacg agtgaagccg gtttatatcg gaaaaccaaa tgccatcatt atggacaagg
    15661 cggttgagca cttaggtttg gaacgtgaag agttaatcat ggttggggac aattatttga
    15721 ctgatatccg ggctgggatt gacaatggca ttccaacgct cttggtgacg acaggtttta
    15781 ccaaggcaga agaagtagct ggcctgccaa tcgcaccgac acatgtggtt tctagccttg
    15841 cggagtggga ttttgatgaa aactaaattg accttttggg gctctatgct ctttctcctc
    15901 tccctctcaa tccttctgac catttatctg gcttggattt tctatcctat ggagattcag
    15961 tggctaaact taacgaatcg agtctatcta aaaccagaaa ccattcaata caattttcat
    16021 atcttgatga attatctgac caatcctttt agtcaggtct tacagatgcc tgattttcgt
    16081 tcgtcagcag ctggtctgca ccatttcgca gtggtcaaga atctctttca tttggttcag
    16141 ctagtagctc tagtgacact gccaagtttc tatgtctttg tcaataggat tgtgaaaaag
    16201 gactttttgt ctctttatcg aaaaagtctc ctggctctag tagtcttacc tgtgatgatt
    16261 ggacttgggg gagttttgat tggttttgac caattcttta ctcttttcca tcaaattctc
    16321 tttgtgggag atgatacctg gctttttgat ccagccaagg atcctgttat tatgattttg
    16381 ccagagacct tctttcttca tgccttcctc ctcttttttg ccctctatga aagcttcttt
    16441 ggctatctgt gtctgaaaag tcgtaggaag tgaaatgaaa gatgttttta atttgcatat
    16501 ataaaaatat atatcgttta aaaccatcgt taagagtgaa ttaaccaaat ccaattgtgt
    16561 caatcgttcg caaacagcct atttttcttg aaaaaatagg ctttttttct aaaaatccct
    16621 agtcaagcgc cttatttttt tgtataatag aagtagcaaa gtatagataa gaagagaaaa
    16681 gcatgattac actatttcta tcaccgagct gtacatcatg tcgtaaggct aaggcctggt
    16741 tagaaaaaca taaggttccc tttgtggaac acaatattat gaccagtcct ttaacaagaa
    16801 aagaattgca acacatcctt tccttgaccg aaaatggtac tgatgacatc atttcaactc
    16861 gttcaaaaat ttttcaaaaa ttgaatattg atgtagagag tatttcggta tcggaattgc
    16921 ttcatttgat tgagcagtat cctagtcttt tgcgtcgtcc aattattatt gatgccaagc
    16981 gtatgcaaat cggttttaat gaagatgaga ttcgtgcttt tctccctcgt agttatcgta
    17041 agcaagaact aaaagaagca agaatgagag ctggtattag ttaatgaaca aacagtatag
    17101 ttacccacta gatttgtcgt ggagcactga agaacttgct tcagtgcttt ctttttttaa
    17161 tgatgttgaa gctgcctatg aaggcaaagt agaggctaaa aagttactgg actcctataa
    17221 gggattcaaa gcggtcgtgc caagcaagag cgaagagaaa cgtttgggac gagaatttga
    17281 aacggttagt ggctattcac tttaccgagc agttcaggct gccaaagaaa aaggggaagg
    17341 aaagatttct cttggaaagt aaatttgaat ttgctaaaga gcttgttaag aaagcaggtc
    17401 agtacattct tgaccatatg caggaagact tgcgtgttga aaccaagtcc tctccaacag
    17461 atttggtgac cagactggac aaagaagttc aggaactctt ggttggtgag attttgtccc
    17521 gttatcctga ggataagatt tgtgctgaag aaggttgtct gcgagcctcg gttcaagagg
    17581 gcaaggtttg ggtcattgat cccattgatg gtaccaataa ttttgtagcc cagcaggaag
    17641 attttgctgt tatgatggct tattttgaaa atggtcaggg acagtttggt ctgatttatg
    17701 atgtggtcaa aggggattgt taccacggtg gtggggaatt tccagtttgt ctaaatgata
    17761 ggcccctagc tccttttaaa actaaaccac ttggagattt tctcattgca gggaatagtg
    17821 gtatgctgga aaccaatgaa tgggggctgg ctgatttgag ccgagcggtt cttggtgttc
    17881 gtgtctatgg aagtgcggcc attagttttg ccaagatttt gtcagggcgt ttgttgacct
    17941 atctcactta tctgcaacca tgggattatg ccgcagctag tattttaggg gaaagtctgg
    18001 gctatcgggt tgtgaccctt tttggtgaag ctcctgattt tcaaaccaga cagccggtca
    18061 tgatggtgcc tcttgagatg caggaggaaa ttcagtccta tatttacgaa aggaaaagaa
    18121 cttaaatgca atttccagaa ggatttgttg aaaaatataa agagatacta ggagatgagg
    18181 caagagattt tcttgcctct tttgaggagg aagcggtttc ggcctttcgg gtcaatccct
    18241 taaaagaaga gcaactttcc ttttctgatg ccattactca aaccccttgg ggccactatg
    18301 ggaaggtttc agggaaatcg cctgagcatg ctacaggttt agtttattcg caagaacctg
    18361 ctgctcaaat ggtggctcaa gtagctcaac ccagtcctgg tatgaaggtc ttggacttgg
    18421 ctgctgcacc ggggggcaaa tcaactcaac tggcagccta tctagcaggg gaggggctac
    18481 ttgtttccaa tgaaatttca agcaaacggg ctaagatttt ggttgaaaac atggagcgtt
    18541 ttggagcgac aaatgtcgtg gtgaccaatg aatctgccga ccgcttggtc aaggtcttta
    18601 aaggctattt tgacttaatc gttcttgatg ccccttgctc tggtgaagga atgtttcgta
    18661 agcaacctga tgctatggac tattggagct tagattatcc gagtcaatgt gctagcttgc
    18721 aaagagaaat tctggaggat gcagtgacca tgttagctga aggtggtcgt ctggtttatt
    18781 cgacctgtac ctgggcaccc gaggaaaacg aagagattgt caattggttg ctggaagagt
    18841 atgattttga cttgttgcca gtagagcata tcaatggaat ggtagctggt attgacctgc
    18901 cagaaacggc tcggatgtac cctcatcagt ttaagggaga gggtcagttt gttgcccatc
    18961 tacagtttaa gggtaacaat ccagctccta aatttaaggc aagtaagagt aacctcagcc
    19021 gtgaacaagt tgccttgtgg caggaatttg cccaaaacca tttgaaggtc aatctaccgg
    19081 gtatcttgca gacttttgga gaccagcttt atctcttgcc agaacttttg ccagatttag
    19141 ggaagctcaa gattgctcgc aatggactgc atctgggcac ttttaagaag aaacgctttg
    19201 agcccagttt cgctcttggt ttagccttga aaccgagtca ggtcgaacag tcggttgaaa
    19261 ttggccaaga agcgtttgtc aagtatgcgg ctggagaaat cgttcagctg gcagaaagtc
    19321 tgccaaatgg ttggtaccaa gttttggtta aaggcaatgg cttgggcttt gcaaaggtta
    19381 ctggaaatgt tttgaaaaat tattttccaa aaggcctcag attcaaatga aaatgctagt
    19441 caaatatctt attctatgtt atagtgaaag tatggatttt tttagtagga tgattcttga
    19501 tatcttgatt gccattacat gtagttttac tactcaatga aaatcaaaga gcaaactagg
    19561 aagctagcca tagatagctc aaaacactgg tttgaagaga ttttcaaaga gtacaagaag
    19621 tttagttatt agcgttctta ccgcttgtaa actagatttc tcataaaata gaatcttttc
    19681 cttttagttg taaactagtc tgggagagta gagaggtttg agataccttt ctagcttttg
    19741 gattatcatc taaggagagt aatttccctt gcattaaaaa ggggaaaaag agacacgaaa
    19801 tgactataat gggtgacaat gggggaaggg atagacaaga gattttatcc acatatgaaa
    19861 aaaggaggtt aggaaagagt aatatagcct atattatata aataatcaat tgcgcagaaa
    19921 tttggtaaga attcatgcgt caactcataa agaactactt aaaaaattca cagtattcat
    19981 aattattttc gaggagaaaa acagtgaaaa aaagaaaaaa gcttgctctg tctcttatcg
    20041 ctttttggct gacggcttgt ttagtaggct gtgctagctg gattgatcgt ggagaatcca
    20101 taacggctgt tggctcaact gccttgcaac ccttggttga agtagcggca gatgaatttg
    20161 gcacgatcca tgttggaaaa acggtcaatg tccaaggggg aggttctggt acaggcttgt
    20221 cccaggttca gtctggggca gttgatatag gaaactcaga tgtatttgct gaggaaaaag
    20281 acggaattga tgcttctgct cttgttgacc acaaggtcgc ggtagctggc ttggctctga
    20341 ttgtcaataa ggaggttgat gttgataacc taacgacaga gcaacttcgt caaatcttca
    20401 taggtgaggt aaccaattgg aaagaggttg gtggtaagga cttacccatc tctgttatca
    20461 atcgggcagc cggctctggt tctcgtgcta cctttgatac tgtcattatg gaaggtcagt
    20521 ctgccatgca aagtcaggag caggattcaa atggagcggt aaaatcaatc gtatcaaaaa
    20581 gtccaggagc tatctcttat ttatctctta cctatataga tgattcggtc aaaagcatga
    20641 agttgaatgg ctatgactta agtccagaaa atataagtag caataattgg cccttgtggt
    20701 cttatgagca tatgtataca ttggggcagc ccaatgagtt ggctgcagaa tttctcaatt
    20761 ttgttctctc ggatgagacc caagaaggga ttgtcaaagg attgaagtat attccgatta
    20821 aggaaatgaa ggttgaaaaa gatgctgccg gaactgtgac agtgttggaa gggagacaat
    20881 aatgaatcaa gaagaattag ctaagaaaat gttgcttcca tcaaagaatt ctcgtctgga
    20941 gaaattagga aaaggtttga cctttgcctg tctttctttg atagtcatcc ttgtggccat
    21001 gattttggtt ttcgtagcgc aaaaaggctt gtcgaccttc tttgtcaatg gtgtgaatat
    21061 ctttgacttt cttttgggag gaacttggaa tccttctagt aaagaatttg gtgcccttcc
    21121 tatgattttg ggttccttta tcgttaccat tctctcagcc cttatcgcaa caccctttgc
    21181 tattggtgca gcagttttta tgaccgaagt atcaccaaaa ggggcgaaga ttttgcaacc
    21241 agctattgaa ctcctggttg ggattccttc agtagtgtac ggatttattg gcttgcaagt
    21301 cgtcgttccc tttgttcgca gtgtctttgg tgggactggt tttgggattt tgtcagggat
    21361 ttccgtcctc tttgtcatga ttttgccgac cgtaaccttt atgacaacgg atagcttgcg
    21421 tgcggttcct cgttattatc gtgaggccag tttcgctatg ggagccactc gctggcagac
    21481 tatctggcgt gtgatcttga aggcggcccg ttctggtatt ttcactgcag tggtctttgg
    21541 gatggcgcgt gcctttggtg aggctttagc tatccagatg gttgtcggaa actcagctgt
    21601 tatcccaact tccttgacca caccagctgc aactttaact tctatattaa ctatgggaat
    21661 tgggaacact gtcatgggaa ctgtaaataa taatgttctc tggtcactgg ccttggtact
    21721 gctcttgatg agtttagttt ttaacagtgt gattaaattg attacgaaag aaagaggaaa
    21781 gaaaaattat gcgcgctaag aaattagata aacttgcaac agctgtcctc tatacgattg
    21841 ctagcatcat tgtgacaatc ttggcttcct taattctcta tatcttggtt cggggcttgc
    21901 cccatatctc ttggtctttc ttgactggaa ggtcttctgc ttttcaagca ggtggtggga
    21961 ttggcattca gctttacaat tcctttttcc tattggtcat taccttgatt atttctgtac
    22021 ctctttctat gggagctggg atttacttgg ctgaatatgc taaaaaaggt cctgttacca
    22081 actttgtgcg gacttgtatt gaaatcttgt cctctttacc atcagtggtg gtgggtctct
    22141 ttggttactt gatctttgta gtccagtttg agtatgggtt ttcaatcatt tcaggtgcct
    22201 tggccttgac agtctttaac ttgcctcaga tgacgcgtaa tgtagaggat agtttgaaac
    22261 acgttcacca tacccaacgt gaggctggtc tggctcttgg gatttctcgc tgggagacag
    22321 tggttcatgt tgttattcca gaagcgcttc caggtattgt aacgggtgtc gtcttggcat
    22381 ctggtcgtat ctttggcgaa gctgcagctc tgatctatac agcagggcaa tcggcgccag
    22441 ctcttgactg gtctaactgg aatatcctca gtgtgactag ccccatctct atcttccgtc
    22501 aagcagaaac cttggctgtc catatctgga aagtcaatag tgaaggcact attccagatg
    22561 gaaccattgt atcagcaggt tctgccgctg tgctcctgat ctttatcctg atttttaact
    22621 ttggagctcg taagttcgga agctatctac acaagaaatt aaccgctgcc taaaggagaa
    22681 gccatgtcaa catataactg ggatgagaag catatcctta cctttcctga agaaaaagta
    22741 gccctttcta ctaaggatgt ccatgtttac tatggtaaaa atgaatccat taaggggatt
    22801 gatatgcaat ttgaaagaaa taaaattaca gctttgattg gtccgtcggg atcggggaaa
    22861 tctacctact tacgcagtct caatcgcatg aatgatacca ttgatattgc taaagtaact
    22921 gggcagattc tctatcgtgg aattgatgtc aaccgtccag aaatcaacgt ttatgaaatg
    22981 cgtaaacaca ttggaatggt ttttcaacgc cccaatccat ttgctaagtc aatttaccgt
    23041 aatattacct ttgcgcatga acgtgctgga gttaaggata agcaagtcct agatgaaatc
    23101 gtagaaacct cccttcgtca ggctgccctc tgggatcagg ttaaagacga tctccacaag
    23161 tcagccttga ccttatcagg tggtcagcaa caacgtctct gtatcgctcg tgccatctct
    23221 gttaagccag atatcctctt aatggatgag ccagcctcag ccttggatcc gattgcgacc
    23281 atgcaactag aagagaccat gtttgagctc aagaaaaact ttaccatcat cattgtaacg
    23341 cataatatgc agcaggctgc tcgtgcaagt gactatacag gcttctttta cttgggtgat
    23401 ttgattgagt atgacaagac tgcaactatt ttccaaaatg ccaagctaca gtccaccaat
    23461 gactatgtat ctggtcactt tggttagaaa ggaaaccgta tgacagatgc gattttacag
    23521 gtatcagacc tgtccgttta ttataataaa aagaaggctt tgaatagtgt ttccctatct
    23581 ttccaaccta aggaaattac agccttgatt ggtccatctg gatcagggaa gtcaaccctc
    23641 ctcaagtctc tcaaccgcat gggagatctc aatccagagg tgaccacaac tggatccgtg
    23701 gtgtacaatg gtcacaacat ctacagtccg cgtacagata cggttgaatt acgtaaggaa
    23761 atcggaatgg ttttccaaca acctaatcct ttccctatga ctatctatga gaatgttgtc
    23821 tacgggcttc gtatcaatgg aattaaggat aagcaggttc tggatgaagc cgtagaaaaa
    23881 gccttgcaag gtgcctctat ctgggatgag gtcaaggatc gtctatatga ttcagctatt
    23941 ggattgtcag gtggtcaaca gcagcgtgtc tgcgtggccc gtgtcttggc aactagtcct
    24001 aaaatcatcc tcttggatga gccaacttcg gctttggatc cgatttcagc tggtaaaatt
    24061 gaggaaacct tgtatggtct aaaagacaag tacaccatgc ttctggtaac ccgttccatg
    24121 cagcaagctt cacgtatctc tgataagaca ggatttttcc tagatggaga tttgattgaa
    24181 tttaatgata ccaagcagat gttccttgat ccccaacaca aggaaacgga agactatatt
    24241 acaggaaaat ttggataagg agatgaaaga tgttacgatc tcaatttgaa gaagatttag
    24301 agaaattaca taaccagttc tacgctatgg gacaagaagt gctctcacaa atcaatcgta
    24361 cggtacgtgc ttttgtcacg catgaccgtg acctggcaaa agaggtcatc gaagatgatg
    24421 cagaagtaaa tgaatacgaa gtgaaactgg aaaagaaatc atttgaaatg atcgcactcc
    24481 aacaaccagt ctctcaagat ttgcgtacag tcttgactgt ccttaaggct gtatcagatg
    24541 tggagcgtat gggggatcac gctgtagcca ttgcccaggc aaccatccgt atgaaggggg
    24601 aagaacgtat tccagctgta gaggaagaaa ttaaaaaaat gggacgtgaa gttaaaagcg
    24661 ttgttgaagc agcacttgat ctttatctta atggttctgt tgacgacgca taccgggtgg
    24721 cctccatgga tgagcaaatt aaccactatt ttgaaactat ccgtgacctt gcgactgaag
    24781 agattaagaa aaatccagaa gccattgtta cgggtcgtga ttatttccaa gttatttcct
    24841 acttggagcg tatcggtgac tatgctaaaa atatctgtga atgggttgtc tactttgaaa
    24901 caggtaagat tgtcgaacta taaaatagtt taattctgta taaaaaggag cttgaaatca
    24961 actgatggct cctttttttg aacgaaaaaa tatttttgag ataaaaattt aaaaaacact
    25021 tgacaagtaa cttggatatc gttataatta tacaaaatta acagagaggt tgtttattta
    25081 tgaaatcaaa aaaatggata tttgttttat gtaattttct tgcaagtttc ttcttagtag
    25141 cttgccagtc aggttctaat ggttctcagt cagctgttga ggcaattaag caaaagggga
    25201 aattagttgt ggcaaccagt cctgactatg caccctttga atttcaatca ttggttgatg
    25261 gaaagaacca ggtagtcggt gcagacatcg acatggctca ggctatcgct gatgaacttg
    25321 gggttaagtt ggaaatctca agcatgagtt ttgacaatgt tttgaccagt cttcaaactg
    25381 gtaaggctga cctagcagtt gcaggaatta gtgctactga cgagagaaaa gaagtctttg
    25441 atttttcaat cccatactat gaaaacaaga ttagtttctt ggttcgtaag gctgatgtgg
    25501 aaaaatacaa ggatttaact agcctagaaa gtgctaatat tgcagcccaa aaagggactg
    25561 ttccagaatc aatggtcaag gaacaattgc caaaagctca attaacttcc ctaactaata
    25621 tgggtgaagc agtcaatgaa ttgcaggctg gaaaagtaga tgctgttcat atggatgagc
    25681 ctgttgcact tagttatgct gctaaaaacg ctggcttagc tgtcgcaact gtcagcttga
    25741 agatgaagga cggcgacgcc aatgccgttg ctcttagaaa aaatagtgat gatttgaaag
    25801 aagtggtaga caaggtcatc caaaaactta aggatgaagg aatttaccaa agctatcttg
    25861 aaaaagcagc aagtctaact gaagttgaag aataagaaaa agcagagttg gatgtcattc
    25921 caattctgct tttaaatagt ttaaaacatt ttccagattg tctagggaca ccttggcaaa
    25981 ttgataagga caggtactta aataggcctc ttcaaagaag tccaagcccg gttgactgtg
    26041 tttgagactg gcgattttag gatactgtct caggtctagc aataaaccat cgtgtagggg
    26101 ataatgagga aggggacaag gtgttttagt tatcctttcc tcgatttgtt tttggtaaga
    26161 ttcatgattg gtcaagcgag ccaaacgatt tttttcaaac aattgactgt caatatagag
    26221 tgacagggcc ttttgcataa tgaggttgct gtcgtagtct aggatatttt tgtaggccac
    26281 aaatgcttct ttgatagcat ttggaagaat gagtgctgta atacgaaggg caggaaaaag
    26341 gctggtcgag aaggacttga tataaatgac atgctcctct gtatcaagat agtggaaggt
    26401 ttggcccttc ttggagtcca aatcacccag ataatcgtct tctacgatat agacatcata
    26461 cttggcagct aagttaagaa tggatcgttt gtcttgctca gaataggaat gtcccagagg
    26521 atagtgaaat cggggaatgg tgtagaaaaa cttaattttt cctgtcttga agtgtcgctc
    26581 cagctccttc aggtcaatcc catcaatgcc tcgttcaatc gtttgatagt ccagcccctg
    26641 tgcaatcaag aggcgattca tccgatggta ggttggctgt tccaccaaga tttccttggc
    26701 ttggctagga aaggatattt gagagaggat aaacaaggct tgttgggttc cagaagtcag
    26761 tactagttgg ttagccttgc agtagagagc ttgctcaaag aggagtttgt gaatggattg
    26821 tcttaggtct tctaatcctt cttgattatc atagtagttg aagaggtagt tttctcggcc
    26881 aatcaaggtt tcattgacac agagtcggaa atcgtcatag gcactggcat gttcgtcggt
    26941 aacctcgatt tctaggtcct ggtgttgccc ttgttctagg acatagtagc cactctgggg
    27001 cttggcatag aggtattgtt cgtgccgtaa ttccagcagg gctcgttgaa tggtgtcctt
    27061 gctacagtga aagtcaaggc tcagttgacg gatagaaggc aggcgactac ccgtcggaaa
    27121 tcgtccagac tcgataccat ttttcagata ggaaacgacc tctttgtact tgctttgttt
    27181 cttcattcca gacctccctt gattttgtta cattgtaccc ttttttttcc ttcttggcaa
    27241 tgtctagagc gtttctctcg ttcacatcta ggggcaaatg tggtatactt aggagtaaga
    27301 tttatagaat aacagacaag aaagtgaatg gataagaacg tgcaggaacc agtgaaatta
    27361 tttcaataca atactcttgg agccttgatg gctggccttt atggtggtac catgacagtg
    27421 ggagaattgc tagagcatgg tgaccttggt ttgggaacct tggattctat tgatggggaa
    27481 ttgattgtct tggatggcaa ggcttatcag gccaaagggt caggagacca gccagaaatt
    27541 gtggaagtgt caccagatgc ccttattcct tacgctgcag tggtaccgca tcaggcagag
    27601 gtcattttcc gccagcgctt tgagatgaca gacaaggaat tgaaagaacg aattgagtct
    27661 tattatgatg gggaaaatct tttccgttct atcaagattc ggggagaatt ttcacatatg
    27721 catgtgcgta tgattcctaa gtcaacaccc gatactaagt ttgctgatgt cgcaacccat
    27781 caaccggaat atagtcgtga caatgttgcg gggactattg ttggtttctg gacgcctgag
    27841 attttccatg gggtcagtgt ggcaggctac catctgcact tcatatcaga tgatttgacc
    27901 ttcggtggac atgtcatgga ttttgtcatc aaggaaggca ttatcgaggt gggagcagtt
    27961 gaccagttgg accaacgttt cccagtccaa gaccgtcaat acttgtttgc taagttcaat
    28021 gttgacgaga tgaaaaaaga tattgaaaag gcagaatagg agaagaaaat gaccattcat
    28081 atcattatta ccatgttgct gttgctagct tttctgatag gaagcatttg gtttgccaaa
    28141 aagaaatacc agattaatct agctgtcttg ggcttgggtg cagtcgcttt ctttgtctct
    28201 tcgcagattt tggaaaaact ggtgcatatc ttgattttgc accctcaaaa agacggtagt
    28261 attgccctct tgcaagatca tccgcttatc tatattatct atggcctagc catggcagca
    28321 ttttttgagg aaacggctcg tcttgttttc ttcaaatggt tggagaaaaa gagaagtttg
    28381 gaaaaggcag atgccttggc ctatggccta gggcatggtg gcttggagtt gatttttcta
    28441 ggccttatta gtttactcaa tctttacatc gttctttcgg cagttcaaac tcagaatcca
    28501 caggttatgc aattactgtc tgaaaatatg ttaaaaacca ttcagtcact ttcggtctgg
    28561 cagatttatt tgcttggttt tgagcgaatc ttggcgcttg gtttccaatt acttttgaca
    28621 gtttgggttt accaagctgt tcgccagaag aaatggattt atctcctagc agcctatggc
    28681 ttgcatgcct tctttgatct ggcaccatct cttttccaag taggctggtt gacaaatcca
    28741 gtcttggttg aagtgattct agcactggag ctcgttctgg tcgcctatgg aaccaaggaa
    28801 atcttttgta aaaaatcata aaaagggggg aacctctttt tcttatgcaa aatccaaaca
    28861 aggtattttt atggtcgtca aatgtctcta aaaatggtat aatggaatga attttgtaaa
    28921 aggaagagtg acatgtctgt aagagaaaaa atgcttgaaa tcttagaagg aattgatatc
    28981 cgttttaagg aacccttgca tagctatagt tatacaaaag taggtggaga ggctgattat
    29041 ttggtctttc cacgaaatcg ttttgagttg gcgcgccttg tgaaatttgc caaccaagaa
    29101 aatatccctt ggatggttct tggcaatgca agcaatatca tcgttcgtga tggtgggatt
    29161 cgtggatttg tcatcttgtg tgacaagctc aataacgttt ctgttgatgg ctataccatt
    29221 gaagcagaag ctggggctaa cttgattgaa acaactcgca ttgccctccg tcatagtttg
    29281 actggctttg agtttgcttg tggtattcca ggaagcgttg gcggtgctgt ctttatgaat
    29341 gcgggtgcct atggtggcga gattgctcac atcttgcagt cttgtaaggt cttgaccaag
    29401 gatggagaaa tcgaaaccct gtctgctaaa gacttggctt ttggttaccg ccattcagct
    29461 attcaggagt ctggtgcagt tgtcttgtca gttaaatttg ccctagcccc aggaacccat
    29521 caggttatca agcaggaaat ggaccgcttg acgcacctac gtgaactcaa gcaacctttg
    29581 gaatacccat cttgtggctc ggtctttaag cgtccagtcg ggcattttgc aggtcagtta
    29641 atttcagaag ctggcttgaa aggctatcgt atcggtggcg tagaagtgtc agaaaagcat
    29701 gcaggattta tgatcaatgt cgcagatgga acggccaaag actacgagga cttgatccaa
    29761 tcggttatcg aaaaagtcaa ggaacactca ggtattacgc ttgaaagaga agtccggatc
    29821 ttgggtgaaa gcaagtagga agctgtattt gaaaagctgc tgctatgtca tggaaagggg
    29881 gtgaaagcct atcggtagcg aagatgtatg caggtggttt tactccctgc aagaggtagt
    29941 ggggacctga cagagccccg atcggttaat ctatgaaaaa gaaggaattt atgccaattg
    30001 aaaaaaccaa ttatcgaatt caaaaacgtc tctaaagttt ttgaagacag caacaccaag
    30061 gttctcaaag acatcaactt tgagttggaa gaagggaaat tctacaccct tctaggtgca
    30121 tctggttcgg ggaaatcaac tatcctaaac attattgcag gtttactgga tgcgacgaca
    30181 ggagatatca tgctagacgg tgttcgtatc aatgatattc caaccaacaa gcgcgacgta
    30241 cataccgtct tccaatccta tgccttgttc ccacatatga atgtgtttga aaatgttgcc
    30301 tttccacttc gcttgcgtaa aattgataag aaagaaatcg agcagcgtgt agcggaagtt
    30361 ctcaagatgg ttcagttgga aggttatgaa aaacgttcca tccgcaaact ttctggagga
    30421 caacgtcagc gtgtggccat cgcccgtgct atcatcaacc aaccccgtgt ggtcttgttg
    30481 gacgagcctt tatcagcgct ggacttgaaa ttgagaacag acatgcagta cgaattgcgt
    30541 gaattacaac aacgattggg cattaccttt gtctttgtca ctcacgatca ggaagaagct
    30601 cttgccatga gtgactggat tttcgttatg aatgatggcg agattgtcca gtctggaacc
    30661 cctgtggaca tctacgatga gccaatcaac cactttgttg ccacctttat cggggagtca
    30721 aacatcttgc caggtaccat gattgaggac tacttggtcg aatttaacgg caaacgcttt
    30781 gaagcggttg atggtgggat gaagccaaat gaacctgttg aggtcgttat tcgtccagag
    30841 gacttgcgca ttacccttcc tgaagaaggc aaactccaag ttaaggtcga cacccagctc
    30901 ttccgtggag ttcattatga aattatcgcc tatgacgaac ttggaaatga atggatgatc
    30961 cactcaaccc gtaaggccat cgtgggtgag gaaatcggtc tggactttga atcagaagac
    31021 atccacatca tgcgtctcaa tgaaaccgaa gaagagttcg atgctcgtat cgaagaatac
    31081 gtggaaatcg aagagcaaga agcaggtctg atcaatgcga tcgaggagga aagagatgaa
    31141 gaaaacaagc tctaaactct ttgtagtgcc ctacatgctt tggattgccc tctttgtatt
    31201 ggcacccttg gtcttgattt tcggacaatc ctttttcaac attgaaggcc agtttagttt
    31261 agaaaattat aaatcttact ttgcgtcaca aaacttgacc tacctcaaaa tgagtttcaa
    31321 ctcagtgctt tatgcaggga ttgtgacctt tgtgaccttg cttatcagct atccaacagc
    31381 cctctttttg acccgtctca agcaccgtca actctggctc atgctgatta tccttcctac
    31441 ctggatcaat ttgctcctta aagcctatgc ttttatcggg atttttggtc aaaatggctc
    31501 tattaaccaa ttcttggaat ttatcggaat tggttcacaa cagttgcttt ttaccgattt
    31561 ttcctttatc tttgtcgcaa gctacatcga gctccccttt atgattttgc cgattttcaa
    31621 tgtcttagac gatatggata ataatctcat caatgctagt tacgaccttg gtgcaactaa
    31681 gtgggagacc ttccgtcatg tcatcttccc tctatctatg aacggtgtgc gaagtggggt
    31741 tcagtcggtc tttatcccaa gtttgagtct cttcatgctg acccgtttga ttggtgggaa
    31801 ccgcgttatc accttgggga cggctattga gcagaatttt ctaaccaatg acaactatgg
    31861 tatgggttca accatcggtg tgattctcat cctgaccatg ttcatcacca tgtgggtgac
    31921 caaggaaagg agagaacgat gaaaaaattt gccaaccttt atctgggact ggtctttctg
    31981 gtcctctacc tgccgatttt ttacttgatt ggctatgcct ttaatgctgg tgatgatatg
    32041 aatagcttta caggttttag ctggactcac tttgaaacca tgtttggaga tgggagactc
    32101 atgctgattt tggctcagac atttttcttg gccttcctat cagccttgat agcgaccatt
    32161 atcgggactt ttggtgccat ttacatctac cagtctcgta agaaatacca agaagccttt
    32221 ctatcactca ataatatcct catggttgcg cctgacgtta tgattggtgc tagcttcttg
    32281 attctcttta cccaactcaa gttttcactt ggctttttga ccgttctatc tagtcacgtg
    32341 gccttctcca ttcctatcgt ggtcttgatg gtcttgcctc gactcaagga aatgaatggc
    32401 gacatgattc atgcggccta tgacttggga gctagtcaat ttcagatgtt taaggaaatc
    32461 atgcttcctt acctgactcc gtctatcatt gcaggttact tcatggcctt cacctattcg
    32521 ctggatgatt ttgccgtgac cttctttgta acaggaaatg gcttttcaac cctatcagtc
    32581 gagatttact ctcgtgctcg caaggggatt tccttagaaa tcaatgccct gtctgctcta
    32641 gtctttctct ttagtattat cctagttgta ggttattact ttatctctcg tgagaaggag
    32701 gagcaagcat gaaaaaaatc tattcatttt tagcaggaat tgcagcgatt atccttgtct
    32761 tgtggggaat tgcgactcat ttagatagta aaatcaatag tcgagatagt caaaaattgg
    32821 ttatctataa ctggggagac tatatcgatc ctgaactctt gactcagttt acagaagaaa
    32881 caggaattca agttcagtac gagacttttg actccaacga agccatgtac actaagataa
    32941 agcagggtgg aacgacctac gatattgcca ttccaagtga atacatgatt aacaagatga
    33001 aggacgaaga cctcttggtt ccgcttgatt attcaaaaat taaaggaatc gaaaatatcg
    33061 gaccagagtt tctcaaccag tcctttgacc caggtaataa attctccatc ccttacttct
    33121 ggggaacctt aggaattgtc tacaacgaaa ccatggtaga tgaagcgcct gagcattggg
    33181 atgacctttg gaagccggag tataagaatt ctatcatgct ctttgatggg gcgcgtgagg
    33241 tgctgggact aggactcaat tccctcggct acagcctcaa ctccaaggat ctgcagcagt
    33301 tggaagagac agtggataag ctctacaaac tgactccaaa tatcaaggct atcgttgcgg
    33361 acgagatgaa gggctatatg attcagaata atgttgcaat cggcgtgacc ttctctggtg
    33421 aagccagcca aatgttagaa aaaaatgaaa atctacgtta tgtggtaccg acagaggcca
    33481 gcaatctttg gtttgacaat atggtcattc ccaaaacagt taaaaaccaa aactcagcct
    33541 atgcctttat caactttatg ttgaaacctg aaaatgctct ccaaaatgcg gagtatgtcg
    33601 gctattcaac accaaaccta ccagcgaagg aattgctccc agaggaaaca aaggaagata
    33661 aggccttcta tcccgatgtt gaaaccatga aacacctaga agtttatgag aaatttgacc
    33721 ataaatggac agggaaatat agcgacctct tcctacagtt taaaatgtat cggaagtagg
    33781 agataaacgt aagaagacga atcagtcaag ctggttcgtt tttaaggaat agaagtcagt
    33841 ttttagagcc ctatcttttt ctattctagt tgtttatttg tttatttgct tatttgcttg
    33901 cctagtagta tactaagcct atactcttca aaccacgtca gcgttgcctt gccgtactca
    33961 agtacagctt gaggctagct tcctagtttg ctttttgatt tttattgagt attaacctta
    34021 cagaaagcgg caacaaataa tgaaaatgtc tacttttttc aaaaaaagtt tctggccaac
    34081 ctttatgatt gttaaccaaa ctgttatact attccattta aaggatggct tggatcgaca
    34141 atacctaaca actgagtcca tatattgggt gataggcacc tttatatttg gaaatattct
    34201 agtagctgtg tttagtaata tgaaaatttg ggataaaaag aaaaatggca gtaagaaaaa
    34261 atatatcctg aaaaagtaat cagagatgta gggaattggt ctaattcaac ggagatcatt
    34321 tccagttgac gcaagattaa aaaaacgata taataagaaa gttgagaatt gattttcact
    34381 tgtcttagtc cagagaaatg gcggtgctgc gagccatcta agctaggaag tcatgctact
    34441 caatcataca attgcataag aacaagagaa tgacaagttc attgaatgaa ggtggtaccg
    34501 cggtttttcg cccttcgtga tatgagcttg tcttttgatt tttggaggtg ttgatgaaaa
    34561 catttcttgt caaacaaaag tttcgtcttg gaggcgaacg cttcgctatc aagaatgaca
    34621 ggggagaaat tgcctatcag gtggagggat cattttttaa gattcctaaa acctttacca
    34681 tctatgatgc ggctggtgaa caagtcagtg agattagtaa agaaatcttg accttgcttc
    34741 ctcgttttga gattcagctt cgggatggct cgagttttgt cattcgtaag aagttgacct
    34801 tctggcgaga taagtatgag tttgataatc taggtcttcg tatcgagggc aatatctggg
    34861 atttgaattt caaattgctg gatgatcgcg atcagttgat tgcggaaatt aagaaggaac
    34921 tcttccatct gacctctacc tataccgtaa cggttcttga agacgcttat gcagacctag
    34981 tcatttccct ctgtgtcgcg attgactatg tggaaatgct ggaaagccaa tcacattaaa
    35041 caagtgaata aggagacaat atgaaacaac tatctagtgc acaagtacgc caaatgtggc
    35101 ttgatttctg ggcgaccaaa ggtcactcag tagaaccatc agtaagtttg gttcctgtaa
    35161 atgacccaac tcttttgtgg atcaactctg gggtagcaac ccttaagaaa tactttgacg
    35221 ggaccattat ccctgaaaat ccatgtatta ccaatgccca aaaggctatc cgtaccaacg
    35281 acatcgaaaa cgtagggaag accgcgcgtc accataccat gtttgaaatg ctggggaact
    35341 tctctatcgg ggattacttc cgtgacgaag ctatcacttg ggcttatgag cttttgacaa
    35401 gccctgaatg gtttgatttc cctgctgaaa aactttacat gacctactat ccagacgata
    35461 aagattctta caaccgctgg attgaagtgg gagtggatcc aagtcacttg attccaattg
    35521 aggacaactt ctgggaaatc ggtgcgggac cttctggacc agatacagaa atcttctttg
    35581 accgtgggga agcctttgac ccagaaaata tcggtcttcg cctgcttgca gaagatattg
    35641 aaaacgaccg ttatattgaa atctggaaca tcgttttgtc acaatttaac gcagaccctg
    35701 ctgttcctcg tagcgaatac aaggaattgc cacataagaa cattgatacg ggcgctggtt
    35761 tggagcgttt ggtggccgtt atccaagggg ctaagaccaa ctttgaaacg gacctcttca
    35821 tgccgattat ccgtgaagtc gagaaattgt ctggtaaggt ttatgaccaa gatggcgaca
    35881 acatgagctt caaggttatc gcagaccaca ttcgttctct ttcatttgct atcggtgatg
    35941 gtgcccttcc aggaaatgaa ggtcgtggtt atgttcttcg tcgtcttctc cgtcgtgctt
    36001 ctatgcatgg tcaaaaattg ggtatcaacg agcctttcct ttacaaattg gttccaactg
    36061 ttggaaaaat catggaaagc tactacccag aagtgcttga gaaacgtgac tttattgaga
    36121 aaatcgttaa gagcgaagaa gaatcatttg cccgtaccct tcactcaggt caacactttg
    36181 cccaaggcat tgtagcagac ttgaaagaaa aaggtcaatc tgttatcgct ggttcagatg
    36241 tcttcaaact ctatgatact tatgggttcc cagttgaatt gactgaagaa atcgctgaag
    36301 aagctggtat gactgtagac cgtgaaggtt ttgaagcagc catgaaagaa cagcaagaac
    36361 gcgcgcgtgc ctcagctgtc aagggtggct caatgggtat gcaaaatgaa actcttcaaa
    36421 acatcactgt agaaagtgtc ttcaactaca atgctagcca attgtcttct aaattggtag
    36481 ctatcgtggc ggacaatgca gaagtagaag ctgtttcaga aggaactgcc tctcttatct
    36541 ttgcggaaac gtcattttat gctgaaatgg gtggacaggt agctgactac ggacaaatct
    36601 tggatgagtc aggtaaggtt gtggctactg tgaccaatgt tcagaaagcc ccaaatggtc
    36661 aagcccttca tacagttgaa gtccttgcac cgcttgcctt gaaccaagaa tataccttgg
    36721 caattgatag caatcgccgt caccgtgtca tgaaaaacca cactgcgact catttgcttc
    36781 acgctgccct tcacaatatc cttggaaacc acgcaacaca ggcaggatct cttaacgaag
    36841 ttgaattcct tcgctttgac tttacccact tccaagcagt aactgctgag gaacttcgtg
    36901 cgattgaaca gcaagtcaac gaaaaaatct gggaagctct tgaagttaag acagttgaaa
    36961 cggatattga cactgctaaa gaaatgggag cgatggccct ctttggtgag aaatacggca
    37021 aggaagttcg tgtcgtgact atcggtgact actctattga actttgtggt ggtacccacg
    37081 ttggcaacac ttctgagatt ggtctcttca aaattgtcaa agaagaagga atcggttcag
    37141 gaactcgtcg tatcttggca gtgactggta aggaagcctt tgaagcctat cgtgaacaag
    37201 aggacgctct taaagctgtt gcagcaacct tgaaagcacc tcaagtcaag gaagtacctc
    37261 acaaggtaga aggacttcaa gaacaacttc gtcaacttca aaaagaaaat gctgagttga
    37321 aagaaaaagc cgcagctgca gccgcaggcg atatcttcaa ggatgtgaag gaagtcaacg
    37381 gtcaccgcta cattgctagt caagtgtctg tatcagatgc cggtgccctt cgtacctttg
    37441 cagataactg gaaacaaaaa gactactctg atcttcttgt cctagttgct gctatcggtg
    37501 acaaagtcaa tgtccttgta gcaagcaaga caaaagacct tcatgcagga aaccttgtca
    37561 aagaattagc accaatcatc gatggacgtg gtggtggtaa accagacatg gccatggcag
    37621 gaggaagcaa ccaagctaag atccaagaat tgttggatgc agtagcaggt aaattgtaat
    37681 caagtaaaga tctatccatt tgggtaggtc tttttgtgaa tacaaaaaag ccaaatccgc
    37741 ttggacctag cttggttatt atgagctatt agatggtatt gactgcccag acacttacgg
    37801 atctagctga gacagggaat tgtccataac cttcctcatc aattgtaact tgaccttggt
    37861 ggttaccaag taaatctaca aaggtttgat tagtccattc ttgaccgaca aacattgact
    37921 tgctgttttc ttggtcattt gagataagga ctgcgattgg ggattgattt tcagcacctg
    37981 aacgtaccca accgatacag ttagcatggt caaagtagtc attttgttct ccataggcca
    38041 aatcttttcg gatggctagg aggcggtcaa ggatttcttt gaaatcttgt tgagcatact
    38101 gccctgaaat cccatagtag tctccgtaaa agacacatgg aaggccgtct tggcgtaaca
    38161 aaatgagggc ataggctgct ggcttgaacc attcttcaac ggtagactca agggcttgtc
    38221 ctcgttgggt atcgtggttg tcgacaaaag tcacagcctt gtcaggcttg agttcaacca
    38281 ggctatctgt gaaaatgcca cgaaggtcat agtttgcgcc agcttgactg gcttcaaaga
    38341 gattctggtg gagacgaaca tcgacaaggt caaagtgttc ttccgttttt tcgagatagt
    38401 ccagattggc ttccttgtct gggttccaaa attcaccaaa aacatagaaa tcgtcaccgt
    38461 atttttcctt catatcgcgg atgaagttgc gcataaagaa agagtcaata tgcttaacgg
    38521 catccaaacg gaaaccagct acaccagtcg tttccatgaa ccaatcagcc cagtcataga
    38581 tgttttggat gacttcagga tgtttaaagt ctaggtcggc atacatgagg tagtcgtagt
    38641 ttccgttttc gttatcgacc aattcctcgt tagcccagcc cttgttgtcc ccttggatca
    38701 gataaatccc agatttactg cgtttggcat cgtagtctgt accggtgaag tggtaccaat
    38761 gccagtggaa gccattatag gtatcttggc gaccatcgaa ggtaaaacta gtccagccat
    38821 tgatggtgaa gggttctcca agttcaactg tacggtctac aggatcaact tcgatgacct
    38881 gaaaggcttc cctgtgatcg gcagcagcct tgtggttgag aactacatcg gccataggtt
    38941 gaattccctg tgctttaagg gcttgaatgg cttgaagata gtcttctttg aaaccatact
    39001 tggtgcggac agtccctttt tggttgaact ctcctaagtc aaataagtca tagaccccat
    39061 agccgacatc tttttcgttg gttgccttga aggctggtgg catccagacg tgactgatcc
    39121 ccagatgagc taggtgtgga gcattttcag ccagacgcgt ccagtgttga ccgtcgtggg
    39181 gcagatacca ttcaaagtat tgcatgagtg tttgattttg catgatgttt cctcttactt
    39241 gtcaatcttg ttctttattc tatcataaag tatctcttgg tgcaaacgtt tgcgtaagat
    39301 agaaggaaac tattttaact acttaaaata aagtattgat ttttttgtat caaagtgcta
    39361 gtatagcgat gtataataca gataaagata ggagttgtcc catgattgaa tttcaaaatg
    39421 ttagtaagtt gtatggtgat aaagaggcct tgagtaatct caatttgcag attgaaaatg
    39481 gagagattat gggcttgatt ggtcataatg gggctggaaa atcgaccacc ataaaatccc
    39541 tagtcagtat catttcaccc agcagtggtc gtattttggt agacggtcag gagttatcgg
    39601 aaaatcgctt ggctattaaa cgaaagattg gctacgtagc agactcgcct gacttatttt
    39661 tacgcttaac ggccaatgaa ttttgggaat tgatcgcctc atcctatgat ctgagtagat
    39721 ctgacttgga ggctagtcta gctaggctat tgaacgtttt tgattttgct gaaaatcgct
    39781 atcaggttat tgaaactctt tctcacggaa tgcgtcagaa agtctttgtc atcggagcac
    39841 tcttgtctga tcccgatatt tgggtcttgg atgaaccctt gactggtttg gatccccagg
    39901 ctgcctttga tttgaaacag atgatgaagg aacatgcaca aaaagggaag acagtcttgt
    39961 tttcaactca tgtcctagag gtggcagagc aagtctgtga tcggattgcc attttgaaaa
    40021 aggggcattt gatttattgt ggtaaggtag aggacttgag gaaagaccac ccagaccagt
    40081 ttttggaaag tatctacctt agtcttgctg gtagaaaaga ggaggttgcg gatgcgtctc
    40141 aaggtcatta aaaaattagt tgatatcaat atcctctatt catctcaaga agctaatctg
    40201 gctaatctac gaaagaagca ggctaagaat cctgggaaaa aagtaaatgt ttctgctaga
    40261 gtcctaagtt cttacatttt ttccagtctc ttgatgctca tctgttttag taatatagcc
    40321 attcattttc cttttgagga aatgcctatt tattttagct cgatggttgc tattttactg
    40381 gtgctggcct tttcaacttc tttcactgcc ttttacaatg tcttttatga gagtaaggac
    40441 ctggcctcct ataggcccta tgcctttaaa gaatcagaga ttataatggc caaaggtctg
    40501 tctgtcctct tgccagctct aactggaatt gtaccaatcc tagcttattt tctagccctc
    40561 tatattagtc tagctccttc tctttggctg ggtttgcctt tgatgctact gtccttgacc
    40621 ttattatttg tctctgttac tttagtgacg gtagtggcag tacatttctt ggctcagact
    40681 agggtcttca gaaagtatca gtctattttt tcgaatgtga tgattgggat aggagttctc
    40741 atacctttaa tatttatctt ctttcttcag tcgacttttg gaagtattgt tgacaaagtt
    40801 agagacattc catttctcct ttatcctctt catatctttt acaaaatagc agtggagcct
    40861 ttttcgacag aagccttagt gggtctgctc gcttggatag gactaactct cttcctgctt
    40921 tatctgacca aaaagaaggt ccttcctcgt ttttatgacg tgatcctgct taacagtgag
    40981 gagaaggtca aaaaagaacg tcgcagcaag gagaggattt caactactaa aaagggcttt
    41041 ttccgtatgg ttttacgcta ccacctcacc ctcttgggac aggggactgg tgtgattaca
    41101 gtgcttttta caagtgcttt ccttccttat ctcatgatga tcagtctgat ttccaaaatc
    41161 cgagattctc agatagttcc agacattcat cctccatact ggttaccctt gttttttgta
    41221 ggagtgttta tagcagttgt caataacaat atcaccagcc tgcattcaat tgccttgtcc
    41281 ttggagaggg aaaatgttga ttttctgaag agtttaccct ttgactttgc tcgttatgtg
    41341 aaagtgaaat tttggattat ctttgctgtt cagtcctttt taccagttct gactttgctc
    41401 ggtctttctc tatatctagg cttgtccatc ctttcgatga tttaccttct tgtagtctgg
    41461 atcattgcca gtgtcatcct ttcttgccac cattacttta aggacgttaa aaatctgtca
    41521 accaattgga gtagcattac ggacctggtg aatcgttcaa atggcatagt cgccatagtt
    41581 ttattgttta tttatagtgc aatcctgatg gcccttgtaa ttgggagcat attcttggtt
    41641 cagtctctct cccctatcct tgccatcaac ttgggagtag gagctcttat cgtcttgctt
    41701 gctcttgcta tttttggcta tcattattac ctgtcacgca tattggcaga aatagaaaaa
    41761 agatgagata gttggttgct tgcttgataa attcaattcg acaaacgctt ttatagtttg
    41821 ttagaatgta gttgtaaatg attaagattg gtaatcaaaa atatagggag gaatgttttg
    41881 aataagaaat ataatatagt tctattattg ttgtttatag tttatttatt gggatatttt
    41941 tcaatttcaa aaacgttaat tcctataatg tgtgtgatcc aagtattttt gatagaacat
    42001 atatttagag ttcgaaatag aatgatgcaa ataggtgaaa ttttaattat tgttgcttct
    42061 ataatattat ttattgatag tatattgagt ttgtaacaaa aggcttctct ataaaatcaa
    42121 attttccaga tacctactga agctgattta tttcttcatc tttcttctac tacctttgtc
    42181 actaaatttg acagacacat attgctgata caacctctct acctataatc tcacggttaa
    42241 cctgaggcta gtttcatagt ttgctatttg attttcgttg ataaaaagaa atgctcctcc
    42301 aagacggaag agcatttttt attgtgattc ttacacattt atttcctagt tttgcaggaa
    42361 gtctagtata ccatttttta aaagaagagt aaaaaagcaa gggtagtttc ttttttgaga
    42421 attgtctaaa agtttgatat aatggtagtt atgaatagaa taagagtcag caaaagggtt
    42481 gaaaagaagc ttgctaaggg gctagtttta ctagaagcca gtgatcttga gaatgtcaat
    42541 cttaaggatc aggaagtaga ggtgcagggt caggaaggaa actttcttgg gactgcctac
    42601 ctttctcagc aaaacaaggg cttgggctgg tttgtcagca aagacaaggt ggccttcaat
    42661 caaactttct ttgaaacgtt gtttagaaaa gccaaagaaa agagaaacgc ctactatcaa
    42721 gatgatttga caactgcctt tcgtctcttt aatcaagagg gagatggctt tgggggtctg
    42781 acagtggacc tttatggcga ctacgccgtc ttttcttggt ataactctta tgtttatcag
    42841 attcgtcaga ctatatcaga agcctttaga caggttttcc ctgaggtttt aggggcttat
    42901 gagaaaatcc gctttaaggg ttttgactat gaatctgccc atgtttatgg tcaagaagca
    42961 cctgactttt tcactgtttt agaaaatggt gtcctgtatc aagtctttat gaatgatggc
    43021 ttgatgacag gaattttcct agaccagcat gaggttcgcg gtagtttagt tgacggcttg
    43081 gctatgggta aatccttgct caatatgttt tcctacacag cggctttttc agtagctgcg
    43141 gccatgggag gagctagcca gacaacttct gttgatctag ccaaacgttc acgagaattg
    43201 tctcaagcgc attttcaggc aaatgggctc agcacagacg agcatcgttt tatagtcatg
    43261 gatgtctttg agtatttcaa atatgccaaa cgcaaagact tgacctacga tgtgattgtc
    43321 ctagatccgc ctagctttgc tcggaataaa aaacaaactt tctctgtggc caaggattat
    43381 cacaagttga tttcccagag tcttgagatt ttaaatccgg gagggattat cattgccagt
    43441 accaatgctg ccaatgtttc ccgtcagaaa tttacagaac aaattgataa aggctttgca
    43501 ggaagaagtt accagatttt aaacaaatat ggtcttccag cagattttgc ctataataaa
    43561 aaagatgaaa gtagtaatta cctcaaggtg attagtatga aggttagtaa atgaaattaa
    43621 tcgtttcagt aatgccaaga agtttagagg aggctcaggc tctggatgcc acgaggtacc
    43681 tggatgccga catcattgaa tggcgtgccg actatctgcc taaagaagcg attttgcagg
    43741 tagctccagc catttttgaa aaattcgcag gccgtgagtt ggttttcacg ctacgaactc
    43801 gctccgaagg tggagaaatc gacctttctc cagaagagta tatccatcta atcaaggaag
    43861 ttgcgcaatt ctatcaacca gactatattg attttgagta ctatagctac aaggatgttt
    43921 ttgaggaaat gctggacttc ccaaatcttg ttttgagtta ccataatttc caagaaacac
    43981 ctgagaacat gatggaaatc ttgtcagagt tgacgatcct aaatccaaaa cttgttaagg
    44041 ttgcggtgat ggctcacacg gagcaggatg tcttagactt gatgaactat acacgaggct
    44101 ttaaaaccct caatcctgaa caggaatatg tgaccatttc tatgggtaag gtgggcaagg
    44161 tctctcgtat cactgcggat gtgactggtt cgagttggtc ttttgccagt ctggatgagg
    44221 ttagtgcccc tgggcagatt tctctagcta gcatgaaaaa aatcagggag attttggatg
    44281 aagcttgatg gctatacacg tttagctgca gttgttgcca atcctattaa gcattctatt
    44341 tctcccttca tccacaatag agcctttgag gcgacagcta ccaacggtgc ttatgtggct
    44401 tgggagattg aagcgagtga cttggcagaa acagtggcca atattcgtcg ctaccagatg
    44461 tttggcatca atctgtccat gccctataag gagcaggtga ttccttattt ggataagctg
    44521 agcgatgaag cgcgcttgat tggtgcggtt aatacggttg tcaatgagaa tggcaattta
    44581 attggatata atacagatgg caagggattt tttaagtgct tgccttcttt tacaatttca
    44641 ggtaaaaaga tgaccctgct gggtgcaggt ggtgcggcta aatcaatctt ggcacaggct
    44701 attttggatg gcgtcagtca gatttcggtc tttgttcgtt ccgtttctat ggaaaaaaca
    44761 agaccttacc tagacaagtt acaggagcag acaggtttta aagtggattt gtgtgcttta
    44821 gagaatgttt ctgaactgca agcaaggatt gccgagtcgg atttgctagt taatgccacc
    44881 agtgtgggca tggatggcca atcctctcct gttcctgaaa acatagtctt accagaaact
    44941 ctcttagttg cagatatcat ataccaaccc tttgaaacac cgtttttgaa atgggctaga
    45001 agtcagggca atccagcagt caatggtctg ggaatgttgc tctatcaagc tgcagaagct
    45061 tttcaactgt ggacaggcaa ggaaatgccg acagaagaga tttggcagtc tttaacagaa
    45121 aaataccaat aaagaaaggg agactctcct atgaaaatca gaatcgatat tcctcatcat
    45181 ccttatgata ttcagattga aaaaggttgt atggcccagg ctggtcagtg gttgcgagaa
    45241 ctctggcaac cgcaaaaggt agtcattgtg acagataacc atgtagcctc tctctatgca
    45301 gagaaggtca agctcagcct agaagatgct ggttttcagg tagctgtttt tgatttctta
    45361 gaaggtgaag aaagaaagaa tttaactact gttcagaaag tctatgaatt tctagtcaag
    45421 caaggtctga ctcgtagcga tggaatcgtt gctcttggtg gtggcgttgt tggggacctg
    45481 gctggatttg tagcctctac ctatatgcgg ggtattcact ttgttcagat tccgactagt
    45541 ttgacagctc aggttgattc ttctatcggt ggaaagacag gcgttaacac accatttgct
    45601 aaaaatatgg tggggacctt tgcccaacca gatggggttc tgattgatcc acttgttctt
    45661 gaaaccctcg gaaaaagaga gttgattgaa gggatgggtg aagttatcaa gtatggcttg
    45721 attgaggatc cagaactgtg ggctctcttg acggaactga atggttctgt tgagagcatt
    45781 ttggaacatg cagagacctt gattgaacat tcttgtcagg tgaagcgcaa gatggtggtt
    45841 gaagatgagt tggacaatgg tattcgtctt tacctcaatt ttggccacac tattggccat
    45901 gccatcgaag cgactgccgg ttatggcaag gtcatgcatg gagaggctgt tgccatggga
    45961 atggtacaga tttccaaggt tgctgaggaa aaaggcctca tgccagctgg cattacccaa
    46021 tctatcacag agatgtgtca gaaattcggc ttgcctgttg actatgaaaa ttgggaagtt
    46081 gacaagcttt atcaggctct tactcatgac aagaaaacgc gtggtaacac cttgaaattg
    46141 gtcttggtgt cagagcttgg ttcagcgatc attcacccag tttctctgga agagatgaaa
    46201 gactacttgg taaaataagg agagtgtatg agatatttaa ctgcaggaga atcacacggc
    46261 ccccgtctaa cagctattat tgagggaatt ccagctggac ttccattgac agctgaggat
    46321 atcaatgagg accttagacg ccgtcagggt ggctacggtc gtggtggtcg tatgaagatt
    46381 gagagtgacc aggttgtctt tacttcgggc gttcgccacg ggaagacgac aggggcgcct
    46441 attactatgg atgtcatcaa taaggaccac cagaaatggc tggacatcat gtctgcggag
    46501 gacattgaag accgccttaa aagcaagcgg aaaattactc atcctcgccc aggtcatgcc
    46561 gatttggttg gggggattaa gtaccgtttt gatgatttgc gaaattcttt ggagcgttca
    46621 tcagctcgtg aaaccaccat gcgggtggca gttggtgcag tagccaaacg cctcttggct
    46681 gagctggata tggagattgc caaccatgtc gtggtctttg gtggcaagga aatcgatgtt
    46741 cctgaaaatc tgacagtcgc tgaaattaag caaagagctg cccagtctga agtttctatt
    46801 gttaaccaag aacgagaaca ggaaatcaag gactatattg accaaatcaa acgtgatggt
    46861 gataccatcg gtggggttgt ggagacagtc gtcggaggcg ttccagttgg tcttggttcc
    46921 tatgtccaat gggatagaaa attggatgca agattggctc aagctgttgt ctctatcaat
    46981 gcctttaaag gggtggaatt tggtcttggc tttgaggctg gttatcgtaa aggcagccaa
    47041 gttatggatg aaattctctg gtctaaagaa gacggttata ctcgccgtac caataatcta
    47101 ggtggttttg aaggtggtat gactaatggg caacccatcg ttgttcgtgg ggtcatgaaa
    47161 cccattccta ctctttataa acctcttatg agtgtggata tcgaaaccca cgaaccttac
    47221 aaggcaaccg tggagagaag tgatccgact gctcttccag ctgcaggaat ggtcatggaa
    47281 gcagttgtag caacggttct ggcgcaagaa atcctcgaaa aattctcatc agataatctt
    47341 gaggaactaa aagaagcggt agccaaacac cgagactata caaagaacta ttaaggagtt
    47401 cctatggcaa aaacaatcta tatcgcaggt cttgggttga ttggagcctc tatggcactt
    47461 ggtatcaaac gcgatcatcc agattatgaa attttaggtt ataatcgtag tcaagcttcg
    47521 agagatatcg ccttgaaaga aggcatgatt gaccgtgcaa cggatgattt tgctagtttt
    47581 gctcctttgg cagatgtcat tatcctcagc ttgccaatca aacaaactat tgctttcatt
    47641 aaggagttgg ccaatttgga tttgcgagaa ggcgttatta tttcagatgc tggttcgacc
    47701 aagtcaacca ttgtggatgc ggcggagcag tatttggctg gcaagtctgt tcgctttgtc
    47761 ggggcccatc ccatggctgg tagtcacaag acaggggctg cttcggcaga tgtcaatctt
    47821 tttgaaaatg cctattatat ctttacacct tcaagcctga caagtcagga cacgcttaag
    47881 gaaatgaagg atctgctttc aggtcttcat gctcgtttta tcgagattga tgccaaggag
    47941 catgatcgtg tcacttctca gattagccat tttcctcata ttttggcttc tagtctcatg
    48001 gagcagactg cggtctatgc tcaagagcat gagatggcaa ggcgctttgc ggcaggtggt
    48061 tttcgagata tgacccgaat tgcggaaagc gagccaggaa tgtggacctc cattctcttg
    48121 tccaatagcg agaccattct ggatagaatt gaggatttca aggaacgttt ggaagtgatt
    48181 ggtcaggcca ttagtaaggg agatgaagag caaatttgga acttttttaa ccaagcgcgt
    48241 gaacaacgcc agaccatgga aatccataag cgtggtggtg tggatagctc ttacgacctc
    48301 tatgttgacg ttcctgatga agaagatgtc atcctgcgga ttttggaact gctacgtggg
    48361 acttccttgg ttaacatcca catcaacgag gaaaatcgtg aggatattca cggaatccta
    48421 caaatttcat ttaaaaatgc tcaagacttg gaaagagctg agcatctcat aacagaaaat
    48481 accgactata cagtcgtcat caaataagga gaagaacatg tcaaatattt acgatagtgc
    48541 aaacgaactc agtcgcggtc tacgcggatt accagaatac aaggctgtta aagcagctaa
    48601 agatgcgatt gcagcagatg ctgaggcaag caaaatcttt acagaatatc ttgcctttca
    48661 agaggaaatt caaaaactag cacatacagg tcaaatgcca gatgcgtcct tccaagctaa
    48721 gatggaaggc tttggtaaac agattcaagg aaatagcctc ttgtcagaat tctttaccaa
    48781 gcaacaacaa ttggccattt acctttctga cattgaaaaa attgtttttg aaccagtttc
    48841 agaattgctg aaataagaaa agaacgatcc taaagtcagg aatttttaag gaatttctgg
    48901 ctttttatga taaaataggt aaaagtattg caagtatgag gtccagtatg aaactaaaaa
    48961 caaacattcg ccatttacat ggtagtatcc gcgtcccagg tgacaagtct atcagccacc
    49021 gttccattat ctttggaagt ttggctgagg gtgagaccaa ggtttatgat attctgcgag
    49081 gtgaagacgt tctttcgacc atgcaggttt ttcgtgacct tggtgttgaa attgaggata
    49141 aagatggggt tattaccatt caaggtgtag gcatggctgg cttaaaagcg ccacaaaatg
    49201 cccttaatat gggaaattct ggcacctcga ttcgcctgat ttcaggtgtc cttgctggtg
    49261 cagatttcga agtagagatg tttggagatg atagtctttc caaacgtcct atggaccgtg
    49321 tgacccttcc actgaaaaaa atgggcgtca gcatttcagg gcaaactgaa cgagacttgc
    49381 ctccccttcg cttaaaaggg acgaaaaacc taagacctat tcattatgag ttgccaattg
    49441 cctctgccca agtcaagtca gccttgatgt ttgcagcctt acaagctaag ggggagtcag
    49501 ttattatcga aaaagagtgc acccgtaatc atactgaaga tatgttgaaa caatttggtg
    49561 gtcatttaag tgtggacggt aagaaaatca cagtccaagg gccacaaaaa ttgacaggac
    49621 agaaggtggt cgtaccagga gatatttcca gtgcagcctt ttggttagtc gcaggtttga
    49681 ttgttccaaa ttctcgtcta gtgctgcaga atgtggggat caacgaaact cgcaccggta
    49741 ttattgatgt cattcgtgcc atgggtggaa aattggaaat aactgaaatc gatccagtcg
    49801 ctaaatcttc taccttgact gttgagtctt ctgacttgaa aggaacagag attggtggcg
    49861 ctttgattcc acgattgatt gatgaattgc ccattattgc cctgcttgcg actcaagccc
    49921 aaggtgtaac agttatcaag gatgctgagg agctcaaggt caaggaaaca gaccgtattc
    49981 aggttgtggc agacgcctta aatagtatgg gagcagatat tactcctacg gcagatggga
    50041 tgattatcaa agggaaatca gctcttcacg gtgctagagt caatacgttt ggtgaccacc
    50101 gtatcggcat gatgacagct atcgcagccc tcttggttgc agatggagag gtggagcttg
    50161 accgtgcaga agccatcaat accagctatc ctagtttctt tgatgatttg gagagcttga
    50221 ttcatggcta aggtgttatt agggtttatg ggggctggaa aatcgactat tgcaagaggc
    50281 ttggacccta attaccttga tatggatgct ctgattgaga agcgcctagg tatgtccatt
    50341 gcgaattttt tcgctgaaaa gggagaagca gcctttcgtc aggtagaatc agaagtccta
    50401 gctgatttac tacaaacaga ccaagtcgtg tcaactggag gaggagtggt tatttctcag
    50461 agaaatcgtg acttactcaa gactaataca gataacatct acctgaaagc agattttgaa
    50521 accctctacc aacgtatcgc agctgataag gacaatcagc gaccgctttt tctaaataat
    50581 agcaaggaag aactagtagc tatttttcaa gaaagacagg cttggtatga ggaagtggct
    50641 agtcgggttt tggatgtgac caagctaagc ccagaggaaa ttatagagga actaagatga
    50701 aaattgctta tctaggtccc aagggatcat tttcacacca cgttgtgcag atagcttttc
    50761 ctcatgagga attgcaggct tttgctaaca ttacagatgt catcaaggct tatgagcaag
    50821 gattagtgga ctattctgtg gtgccagttg aaaattctat tgagggtagt gttcatgaaa
    50881 ccttggacta tctttttcat caggctcaca tccaagcagt agcagaaatc gttcagccta
    50941 ttcatcagca gttgatggtg gttccaggcc atactaagat tgaaaagatt ttttcacatc
    51001 cacaggcctt ggctcaagga aagaaattca tcgatgaaca atatccagag gctcaaatcg
    51061 aggtaacagc tagtacagct tatgcggccc gttttatttc cgaacatcca gaccagccct
    51121 ttgcagcagt tgcacctaga agttctgctg aagaatatgg attggaactg attgccgagg
    51181 atattcagga aatggaagcc aatttcacac gtttctgggt tctaggagct gaaaagccta
    51241 gtattccctt gcaagcacaa actgaaaaga tgagtttggc cttgacatta cctgacaacc
    51301 ttccaggtgc actttataag gccctgtcga cctttgcttg gcgaggaatt gacttgacaa
    51361 aaattgaaag tcgtccactc aagacagcac tgggtgaata ctttttcatt atcgatgtgg
    51421 attataccga taaggacttg gtccactttg cccaaaaaga attagaagcg attggaatcc
    51481 agtataaaat tctgggtgcc tatcctattt atccaatatc agaccatgga aaggagagaa
    51541 gatgagtaaa gaaaatccct taagtcatca tgagcagttg cgttatgatt atttgctaaa
    51601 aaatattcac tatctcaatg agagagaaaa aaatgagttt gcctatttgc aagaaaagct
    51661 aactcttgct aggggaaata gtagctctag cttggaacaa gaaagagaag agcaggttga
    51721 cttaccaagc tatgcgaacc ggagtcgctc acaatccaaa tcacaagcac tctctttccc
    51781 tccaaaaaag aaaagacgga agctccgtct taaacgaatt tttatggtga ttttttcact
    51841 tttggtctgt gtggctttgg ccatggtatt catgtttttg cgtggttacc aagatgctag
    51901 cgcaaagaaa actgctgatg cccgggcagc tcaagtagaa gtctttaatg gtcaggacac
    51961 tagagatgga gttaatattt taatcatggg tactgatggt cgaatcggcc agaacagtgt
    52021 tgagacacga actgactcta ttatggtatt aaatgtcggg ggctcagata agaaaatgaa
    52081 gctagtcagt ttcatgcgtg ataatttggt ctatatagat ggttatagtc aagtgattaa
    52141 cggtagaaaa cagacagata acaagttaaa cgtagcctac gagttaggag aacaagaggg
    52201 gcaaaaaggg gcagaaatgg ttcgccaagt cttgaaagat aattttgact tggacattaa
    52261 gtactacgcc ttggttgatt tccaggcctt tgccacagcg attgacacgc ttttccctga
    52321 tggggtgacg attgatgctc aattttcaac attgaatggg cgtcccctaa cagaagctac
    52381 agtcggagat gatttatacg ctaccgagac tgagtctcca acccaaacca ttaaagtcgg
    52441 aaaacagcag atgaatggct cgaccctgct caattatgct cgtttccgtg atgatgatga
    52501 ggcggattac ggccgtacaa aaagacagca acaagtttta acagcaattt tagagcaaat
    52561 taaagatccc actaaacttt tcactggttc tgaagctctt ggaaaggttt ttgctatgac
    52621 ctcaaccaac gtaccctata ctttcctcct aacaaacggt ttatctgttt tggatggagc
    52681 aaaaaatggt attgaaaaat tgacgattcc agaactgggt gactgggtag atgcctatga
    52741 tgtttatgga ggcttgggct tgctggttga tcaaaacaaa tatcaaacca agctcgctca
    52801 aatgggcttg agataagata atactcaatg aaaatcaaag tgcaaactag gaagctagcc
    52861 gcaggctgct aaaaacacag ttttgaggtt gtagataaga ctgacgaagt cagttaccta
    52921 tacctacggc aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt ataagacaga
    52981 aaaatcaaag tttgatagct atttggatag cagtcaggct ttgatttttt acagtctgca
    53041 atagattttc accccttgtt tgtggtataa tgaaacgata cgatagaaag aataatgaac
    53101 aatatgactg atttaaaagc aattcaggct cgtagtctgg agatggctga atattttgtg
    53161 gccttttgta aagaacatga tttgctctgt tatctctgtg gcggaggcgc tattggtgcc
    53221 cttcgaaaca agggatttat tccttgggac gacgacctag acttttttat gcctcgtaaa
    53281 gattatgaga aattagcaga attatggcct cgttatgcag atgaacgtta tttcttgtca
    53341 aagagtcaca aggattttgt tgatcgtaat ctttttatta ccattcgtga caaggaaacc
    53401 acctgtatca agccttatca gcaggatttg gatttgccac atggtctggc cttggatgtt
    53461 ttgcctttgg attattatcc gaaaaatcca gctgagcgga aaaaacaggt tcgttgggcc
    53521 ttgatttatt cactcttttg tgcgcaaact attccagaaa agcatggtgc tctcatgaaa
    53581 tggggaagtc gcattttact gggtttgact ccaaaatctc tctgttatcg catctggaaa
    53641 aaagctgaga aagaaatgac taagtatgat ttggctgatt gtgatggcat tacagaatta
    53701 tgctcaggtc ctggctacat gagaaacaag tacccaatca catcttttga agacaatctt
    53761 ttcttgccat ttgaaggaac agagatgcct attccaatcg gctatgatgt ctatctcaga
    53821 actgcttttg gggattatat gacgcctcca ccagcagaca agcaggtacc gcatcatgat
    53881 gctgtcatcg ctgatatgga taagtcttat acagaataca agggagaata tggtggctaa
    53941 gaaaaaaatc ttatttttta tgtggtcttt ttctcttgga ggtggtgcag agaagattct
    54001 atcaaccatt gtttcaaatc tggatccaga aaagtatgat attgatattc ttgaaatgga
    54061 gcactttgac aagggatatg aatctgttcc aaagcatgta cgcattttaa aatcccttca
    54121 agattatcgc caaaccagat ggttacgagc ttttttgtgg agaatgagaa tttattttcc
    54181 aagactggct cgtcgtttgc ttgtaaaaga tgattatgat gttgaagttt cttttaccat
    54241 tatgaatcca ccactgttgt tctctaaaag aagagaagtc aagaagatat cttggattca
    54301 tggaagtatt gaagaacttc ttaaggatag ctctaaaaga gaatcacata gaagccagtt
    54361 ggatgctgcg aatacaattg tagggatttc aaaaaagacc agcaattcta tcaaggaagt
    54421 ttatccagat tatacttcta aattacagac aatctacaat ggatatgatt ttcagactat
    54481 tctagaaaaa tctcaagaga agatcgatat cgagattgct cctcaaagta tctgtactat
    54541 cggacggatt gaggaaaata agggttctga ccgtgtagtg gaagtgatac gattattaca
    54601 ccaagaggga aaaaactatc atctctattt tatcggggct ggtgatatgg aagaggaact
    54661 gaaaaaacga gtcaaagagt atgggattga ggactatgta catttccttg gttatcaaaa
    54721 aaatccttat cagtatctat ctcagacgaa agttcttttg tctatgtcta aacaagaagg
    54781 ttttcctgga gtgtatgtgg aggccttgag tctgggactc ccttttatct ctacgaacgt
    54841 tggaggggct gaggaattat cccaagaagg acgatttgga caaatcattg agagcaatca
    54901 agaggcagct caggcgatta ctaattacat gacttctgcc tcaaactttg atgtcgatga
    54961 ggctagccaa ttcattcaac aatttacaat tacaaaacaa atcgaacaag tagaaaaact
    55021 attagaggag tagcatggaa actgcattaa ttagtgtgat tgtgccagtc tataatgtgg
    55081 cgcagtacct agaaaaatcg atagcttcca ttcagaagca gacctatcaa aatctggaaa
    55141 ttattcttgt tgatgatggt gcaacagatg aaagtggtcg cttgtgtgat tcaatcgctg
    55201 aacaagatga cagggtgtca gtgcttcata aaaagaacga aggattgtcg caagcacgaa
    55261 atgatgggat gaagcaggct cacggggatt atctgatttt tattgactca gatgattata
    55321 tccatccaga aatgattcag agcttatatg agcaattagt tcaagaagat gcggatgttt
    55381 caagctgtgg tgtcatgaat gtctatgcta atgatgaaag cccacagtca gccaatcagg
    55441 atgactattt tgtctgtgat tctcaaacat ttctaaagga atacctcata ggtgaaaaaa
    55501 tacctgggac gatttgcaat aagctaatca agagacagat tgcaactgcc ctatcctttc
    55561 ctaaggggtt gatttacgaa gatgcctatt accattttga tttaatcaag ttggccaaga
    55621 agtatgtggt taatactaaa ccctattatt actatttcca tagaggggat agtattacga
    55681 ccaaacccta tgcagagaag gatttagcct atattgatat ctaccaaaag ttttataatg
    55741 aagttgtgaa aaactatcct gacttgaaag aggtcgcttt tttcagattg gcctatgccc
    55801 acttctttat tctggataag atgttgctag atgatcagta taaacagttt gaagcctatt
    55861 ctcagattca tcgtttttta aaaggccatg cctttgctat ttctaggaat ccaattttcc
    55921 gtaaggggag aagaattagt gctttggccc tattcataaa tatttcctta tatcgattct
    55981 tattactgaa aaatattgaa aaatctaaaa aattacatta gaacgggggt gaccaagtga
    56041 ttgatgggaa acgattatta tttagtttga ccatagtcag ttatgccttg acgctagtaa
    56101 gtggaattgt gtatctgttt aataataata atgttagctt actttctact ttattgttct
    56161 tgttggttag tagcttaatt gcttgttgga atgatatcaa gtattactta atccatttta
    56221 ttttctattt aaccattttt gtatttctgg tatcaagacc gaccattgat tattttaggg
    56281 atggtgcttt ggatacctat catccaatag cctatcgttt tgcctttata gttgtcatgg
    56341 tttcgattct gggcttgacc acaggaggca ttctggctcg ttacttcata gctaggaaga
    56401 aaataaaagt agcaaatata ggaaattctc taaaagaggt ttatatcaag cggttacgct
    56461 ttgtatcact aggagttttt cttctaacct atcctttcta tttcattcgg ttatttgaac
    56521 ggctcttgta tcgtttgcag acttcctact atgcctacta tgcaaatttt gaaagtaaac
    56581 tgccttattt tacctacatt ttgtctacct ttacggtcta tgcaatgtgt atgtatctgg
    56641 caaccaagcc aaagaaattg caggccacag cagtgcttgt ctcctttatt gcagctaata
    56701 ctattcattt ggcaattggg acacgaaatc cctttatttt aagtatttta tttgcttttg
    56761 tttattactt tatgcgggag caaactgaaa aaggaaaatg gattgggttt aaagaaaagt
    56821 tagcgatttt tgtaggttct cctattctca tgttagcgat gggagtactc aattatgtac
    56881 gggataatgt ccaagtttct catacaggtt tctgggatat cttacttgac tttatctata
    56941 aacaagggac tagttttggt gttctggctc gaggttttct atttaacagt agcctccctt
    57001 accgagattt ccgtaatttt acttttggtc ctgttcttga ttattttgca agggggagtt
    57061 tgggagccat tttcggagga aaagcctttg aacatacaac caatagtgtg gaactagcta
    57121 ttgatagtaa tagttatgcc cacaatctat cctatcttgt cttgaacaag gaatacttga
    57181 aagggcatgg tatcggaagt agttatatca tggagttgta taccgactat ggtatgattg
    57241 gagtctttct gcttagtttc ttactcggcg tattatttat agccatgctg caagtagcct
    57301 atcgctcaag gacaatccta tttgctttat ccctactcat cttgaataat ctattcttta
    57361 tgccaagaag cagcttttca gaaagtttct tcaatttatt tacaatgcaa ttctggggaa
    57421 ttgttcttgt gattatattt gtagcaaaaa tgcttacaaa ggaaaactag tatctactaa
    57481 acaaaggaga aaaaaatcat gtttgaacat tattcagtag ctgatttgtt tgcaaatctt
    57541 tataaaaagc gaaaagcaaa tattttagct ctcatcgctt tatttgctct cattgctgta
    57601 ccatttacaa ttaaagcagt taggaataaa aacactgtca aagatacaac aagttattca
    57661 acttatctta tctataaaat cactcctcca aaagagtcgg ccaaaacgat tttgaatcat
    57721 caaattggtg gttatagtga tttttatggg aaattgattg atggtaattt gaatggagct
    57781 tatcttttca atgatgtaga acccagtgag ttgaaaaaaa ttgccagtga attagacacg
    57841 acagaaacaa ccttgaaaaa ttcaacgaat gactattggt ggaaaaaatt gaccgtctac
    57901 tatatgattg acgatgcagg ggttggtgtg aaaattttga catcaagtaa agatgccaat
    57961 gacttgttag agaaaaaaat tgatgggttg attgagaaat ttaaacatgc ctatgcaaat
    58021 gtgaaaattg aaaaactgga aaccatcaac tctaaagaat tgaacgcaaa tggtgaaaca
    58081 gcgcttggct taaatgtgaa aaatctgatt cttcgtttag ttgttattgg agtggtttgt
    58141 gtgattttgg ttgtgatggg aaatgtgtta gtttatctct ttaatccaac aatcaataga
    58201 gtaggtgatt tttctcagta tcaaattgat tttgtaacag agattacaac aattgctaac
    58261 ctagcagatg ttttgtcata caaaaatact ggacaggaat tgaccatcgt tagctcaaat
    58321 aaagctatcc tagataaatt gaaacagagt caagaagctt taaaaggaat gcattttgta
    58381 gatttacagg atgtatcatc tcttttggaa agagatacag tccttcttgt tgaagagtac
    58441 ggagtgactc gttataagaa atttgagcaa agtcttcaaa ttctcagaaa cttaaatcgt
    58501 tctatccttg gtgtagcaac ctttaaatta taagtattct gatgtataag gtcttcaaaa
    58561 tcggctaaga gctgattttg aaggcttttt ctatctgtct gcagaaattt tcttttggag
    58621 tctcctatag aaagacccta gggaaaatct atccgtatag aggagccttc ttttgataaa
    58681 atcgtaaaaa tctagtataa tagatagaac tgaaagtatg aggttactag caatgaaaat
    58741 gaaacaaatt agtgatacaa ctttaaaaat cacgatgtct ttagaggatt tgatggatcg
    58801 tggaatggag attgctgact ttctcgttcc tcaagaaaaa acagaagagt tcttttatgc
    58861 tatcttggat gaactagaga tgcctgatag ctttctggat acaggcatgt tgagcttccg
    58921 tgtgactcca aaacctgata aggtagatgt ctttgtgacc aagtcaaaga ttgaccaaaa
    58981 tctagatttt gaagacttat cggatttgcc agatatggaa gaattggctc aaatgtctcc
    59041 agatgaattt atcaaaaccc tggaaaaaag catcgcagac aaaaccaagg atgatatcga
    59101 agccattcaa tctcttgagc aagttgaagc caaggaagaa gagcaagagc aggctgaaca
    59161 agaagctgag agtaagaaag aaccttacat ctactacatc ctttcttttg ctaagttggc
    59221 tgacttggta gtttttgcca agacagtgac ttttgaaatg gaaacttctg aactctacaa
    59281 aatgaacgag cgctattatt tgaccatttt agtggatatt gaaaatcatc caagcccata
    59341 tccagcttgg ctgttggccc gtatgcgcga gtttgcagac gatagtgata tcagtcgctc
    59401 agtcttacaa gagtatggtc aagtcttgat gagtcacgat gcagtgctca atctgcaaaa
    59461 aatcggctaa tcctttctgg aaagctattt tcagatttta agaagagcga atcgtctgat
    59521 tcgttttttc tttttatact gaaatagtga tttactataa taggaatttt cacaaaattc
    59581 tgttataatg gctatattag aaaatttcga ggagataaag atgacagtta aaattgcttt
    59641 actaggattt ggtaccgttg caagtggtgt gcctttcctc ctaaaggaaa atggaggaaa
    59701 aatcaatcaa tcagcacatt cagatatcaa agttgctaag gtattggtca aggatgaaga
    59761 tgaaaaaaat cgcttgcttg cagcagggaa tgactttaac tttgtaacca atgtggatga
    59821 tattttatca gaccaggata ttactatcgt agtggaattg atggggcgta ttgagcctgc
    59881 taaaaccttt atcactcgtg ccttggaagc tggaaaacac gttgttactg ctaacaagga
    59941 ccttttagct gtccatggcg cagaattgct agaaatcgct caagctaaca aggtagcact
    60001 ttactacgaa gcagcagttg ctggtgggat tccaattctt cgtactttag caaattcctt
    60061 ggcttctgat aaaattacgc gcgtgcttgg agtagtcaac ggaacttcca acttcatggt
    60121 gaccaagatg gtggaagaag gctggtctta cgatgatgct cttgcggaag cacaacgtct
    60181 aggatttgca gaaagcgatc cgacgaatga cgtagatggg attgatgcag cctacaagat
    60241 ggttattttg agccaatttg cctttggcat gaagattgcc tttgatgatg tagcccacaa
    60301 gggaatccgc aatatcacac cagaagacgt agctgtagct caagagtttg gttacgtagt
    60361 gaaattggtt ggttctattg aggaaacttc ttcaggtatt gctgcagaag tgactccaac
    60421 cttcctacct aaagcgcacc cacttgctag tgtgaatggc gtaatgaacg ctgtctttgt
    60481 agaatctatc ggtattggtg agtctatgta ctacggacca ggtgcgggtc aaaaaccaac
    60541 tgcaacaagt gttgtagctg atattgtccg tatcgttcgt cgtttgaatg atggtaccat
    60601 tggcaaagac ttcaacgaat atagccgtga cttggtcttg gcaaatcctg aagatgtcaa
    60661 agcaaactac tatttctcaa tcttggctcc agactcaaaa ggtcaggtct tgaagttggc
    60721 tgaaatcttc aatgctcaag atatttcctt taagcaaatc cttcaagatg gcaaagaggg
    60781 tgacaaggcg cgtgtcgtta tcatcacaca caagattaat aaagcccagc ttgaaaatgt
    60841 ctcagctgaa ttgaagaagg tttcagaatt cgacctcttg aataccttca aggtgctagg
    60901 agaataagat gaagattatt gtacctgcaa ccagtgccaa tatcgggcca ggttttgact
    60961 cggtcggtgt agctgtaacc aagtatcttc aaattgaggt ctgcgaagaa cgagatgagt
    61021 ggctgattga acaccagatt ggcaaatgga ttccacatga tgagcgtaat ctcttgctca
    61081 aaatcgcttt gcaaattgta ccagacttgc aaccaagacg cttgaaaatg accagtgatg
    61141 tccctttggc gcgcggtttg ggttcttcca gctcggttat cgttgctggg attgaactag
    61201 ccaaccaact gggtcaactc aacttatcag accatgaaaa attgcagtta gcgaccaaga
    61261 ttgaagggca tcctgacaat gtggctccag ccatttatgg taatctcgtt attgcaagtt
    61321 ctgttgaagg gcaagtctct gctatcgtag cagactttcc agagtgtgat tttctagctt
    61381 acattccaaa ctatgaatta cgtactcgcg acagccgtag tgtcttgcct aaaaaattgt
    61441 cttataagga agctgttgct gcaagttcta tcgccaatgt agcggttgct gccttgttgg
    61501 caggagacat ggtgaccgct gggcaagcaa tcgagggaga cctcttccat gagcgttatc
    61561 gtcaggactt ggtaagagaa tttgcgatga ttaagcaagt gaccaaagaa aatggggcct
    61621 atgcaaccta cctttctggt gctgggccga cagttatggt tctggcttct catgacaaga
    61681 tgccaacaat taaggcagaa ttggaaaagc aacctttcaa aggaaaactg catgacttga
    61741 gagttgatac ccaaggtgtc cgtgtagaag caaaataaag aatagaagat aggatgggga
    61801 aactcttaac cagagggttt catatccttt ttgtgaaaag aagtttatac tcaatgaaaa
    61861 tcaaagagca aactaggaag ctagccgcag gctgctcaaa aca
//
LOCUS       DAVKFH010000006        60074 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKFH010000006 DAVKFH010000000
VERSION     DAVKFH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 60074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.006"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(54..803)
                     /locus_tag="VW006_000367"
     CDS             complement(54..803)
                     /locus_tag="VW006_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9379282.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            936..3272
                     /locus_tag="VW006_000368"
     CDS             936..3272
                     /locus_tag="VW006_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9379283.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLMETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDSIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3374..4051
                     /locus_tag="VW006_000369"
     CDS             3374..4051
                     /locus_tag="VW006_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9379284.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4038..4835
                     /locus_tag="VW006_000370"
     CDS             4038..4835
                     /locus_tag="VW006_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9379285.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4852..5955
                     /locus_tag="VW006_000371"
     CDS             4852..5955
                     /locus_tag="VW006_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9379286.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            6256..7275
                     /gene="galE"
                     /locus_tag="VW006_000372"
     CDS             6256..7275
                     /gene="galE"
                     /locus_tag="VW006_000372"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9379287.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7277..8230
                     /locus_tag="VW006_000373"
     CDS             7277..8230
                     /locus_tag="VW006_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9379288.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(8292..8498)
                     /locus_tag="VW006_000374"
     CDS             complement(8292..8498)
                     /locus_tag="VW006_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9379289.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8550..9026
                     /locus_tag="VW006_000375"
     CDS             8550..9026
                     /locus_tag="VW006_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9379290.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9035..9706
                     /locus_tag="VW006_000376"
     CDS             9035..9706
                     /locus_tag="VW006_000376"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9379291.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9837..10175
                     /locus_tag="VW006_000377"
     CDS             9837..10175
                     /locus_tag="VW006_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9379292.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10178..10807
                     /locus_tag="VW006_000378"
     CDS             10178..10807
                     /locus_tag="VW006_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9379293.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11047..12198
                     /locus_tag="VW006_000379"
     CDS             11047..12198
                     /locus_tag="VW006_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9379294.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12511..13260
                     /gene="truA"
                     /locus_tag="VW006_000380"
     CDS             12511..13260
                     /gene="truA"
                     /locus_tag="VW006_000380"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9379295.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13250..14023
                     /locus_tag="VW006_000381"
     CDS             13250..14023
                     /locus_tag="VW006_000381"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9379296.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14001..14462
                     /locus_tag="VW006_000382"
     CDS             14001..14462
                     /locus_tag="VW006_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9379297.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            14689..16030
                     /locus_tag="VW006_000383"
                     /pseudo
     CDS             14689..16030
                     /locus_tag="VW006_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(16158..16361)
                     /locus_tag="VW006_000384"
     CDS             complement(16158..16361)
                     /locus_tag="VW006_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379298.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(16354..17436)
                     /locus_tag="VW006_000385"
     CDS             complement(16354..17436)
                     /locus_tag="VW006_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9379299.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(17562..18344)
                     /locus_tag="VW006_000386"
     CDS             complement(17562..18344)
                     /locus_tag="VW006_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9379300.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(18344..19687)
                     /locus_tag="VW006_000387"
     CDS             complement(18344..19687)
                     /locus_tag="VW006_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9379301.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(19786..21102)
                     /locus_tag="VW006_000388"
     CDS             complement(19786..21102)
                     /locus_tag="VW006_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9379302.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            21295..22521
                     /locus_tag="VW006_000389"
     CDS             21295..22521
                     /locus_tag="VW006_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9379303.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            22720..24294
                     /locus_tag="VW006_000390"
     CDS             22720..24294
                     /locus_tag="VW006_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9379304.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            24559..25347
                     /gene="codY"
                     /locus_tag="VW006_000391"
     CDS             24559..25347
                     /gene="codY"
                     /locus_tag="VW006_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9379305.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            25347..25922
                     /locus_tag="VW006_000392"
     CDS             25347..25922
                     /locus_tag="VW006_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9379306.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            26004..26089
                     /locus_tag="VW006_000393"
     tRNA            26004..26089
                     /locus_tag="VW006_000393"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26038..26040,aa:Leu,seq:aag)
     gene            26373..27503
                     /gene="ugpC"
                     /locus_tag="VW006_000394"
     CDS             26373..27503
                     /gene="ugpC"
                     /locus_tag="VW006_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9379307.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            28004..28762
                     /locus_tag="VW006_000395"
     CDS             28004..28762
                     /locus_tag="VW006_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9379308.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            28850..29362
                     /locus_tag="VW006_000396"
     CDS             28850..29362
                     /locus_tag="VW006_000396"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9379309.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            29544..30488
                     /locus_tag="VW006_000397"
     CDS             29544..30488
                     /locus_tag="VW006_000397"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9379310.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            30497..31174
                     /locus_tag="VW006_000398"
     CDS             30497..31174
                     /locus_tag="VW006_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9379311.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            31272..32030
                     /locus_tag="VW006_000399"
     CDS             31272..32030
                     /locus_tag="VW006_000399"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9379312.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            32068..33573
                     /gene="lytC"
                     /locus_tag="VW006_000400"
     CDS             32068..33573
                     /gene="lytC"
                     /locus_tag="VW006_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9379313.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            34071..34589
                     /locus_tag="VW006_000401"
     CDS             34071..34589
                     /locus_tag="VW006_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9379314.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLCVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            34719..35225
                     /locus_tag="VW006_000402"
     CDS             34719..35225
                     /locus_tag="VW006_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9379315.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            35225..35395
                     /locus_tag="VW006_000403"
     CDS             35225..35395
                     /locus_tag="VW006_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379316.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            35427..36659
                     /gene="clpX"
                     /locus_tag="VW006_000404"
     CDS             35427..36659
                     /gene="clpX"
                     /locus_tag="VW006_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9379317.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            36668..37255
                     /locus_tag="VW006_000405"
     CDS             36668..37255
                     /locus_tag="VW006_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9379318.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHAPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            37266..37646
                     /locus_tag="VW006_000406"
     CDS             37266..37646
                     /locus_tag="VW006_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9379319.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            37698..38588
                     /gene="rapZ"
                     /locus_tag="VW006_000407"
     CDS             37698..38588
                     /gene="rapZ"
                     /locus_tag="VW006_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9379320.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            38585..39562
                     /locus_tag="VW006_000408"
     CDS             38585..39562
                     /locus_tag="VW006_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9379321.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            39559..40470
                     /gene="whiA"
                     /locus_tag="VW006_000409"
     CDS             39559..40470
                     /gene="whiA"
                     /locus_tag="VW006_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9379322.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(40504..41472)
                     /locus_tag="VW006_000410"
     CDS             complement(40504..41472)
                     /locus_tag="VW006_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9379323.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEDYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQTLQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            41608..42465
                     /locus_tag="VW006_000411"
     CDS             41608..42465
                     /locus_tag="VW006_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9379324.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            42452..43231
                     /locus_tag="VW006_000412"
     CDS             42452..43231
                     /locus_tag="VW006_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9379325.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            43255..44607
                     /locus_tag="VW006_000413"
     CDS             43255..44607
                     /locus_tag="VW006_000413"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9379326.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            44681..45055
                     /locus_tag="VW006_000414"
     CDS             44681..45055
                     /locus_tag="VW006_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9379327.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            45057..45905
                     /locus_tag="VW006_000415"
     CDS             45057..45905
                     /locus_tag="VW006_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9379328.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            46182..46949
                     /locus_tag="VW006_000416"
     CDS             46182..46949
                     /locus_tag="VW006_000416"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9379329.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            46961..48145
                     /locus_tag="VW006_000417"
     CDS             46961..48145
                     /locus_tag="VW006_000417"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9379330.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVIKETYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            48142..50013
                     /locus_tag="VW006_000418"
     CDS             48142..50013
                     /locus_tag="VW006_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9379331.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAQDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(50156..51340)
                     /gene="mntE"
                     /locus_tag="VW006_000419"
     CDS             complement(50156..51340)
                     /gene="mntE"
                     /locus_tag="VW006_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9379332.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            51644..54340
                     /locus_tag="VW006_000420"
     CDS             51644..54340
                     /locus_tag="VW006_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9379333.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            54353..55144
                     /gene="yghU"
                     /locus_tag="VW006_000421"
     CDS             54353..55144
                     /gene="yghU"
                     /locus_tag="VW006_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9379334.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLRTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            55260..55670
                     /locus_tag="VW006_000422"
     CDS             55260..55670
                     /locus_tag="VW006_000422"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9379335.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            55682..57277
                     /locus_tag="VW006_000423"
     CDS             55682..57277
                     /locus_tag="VW006_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379336.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGRTFEGQVGKDSYLKTFKVQ"
     gene            57456..57620
                     /locus_tag="VW006_000424"
     CDS             57456..57620
                     /locus_tag="VW006_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9379337.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKENKDKFLSILTSLAGLALV
                     LVAVWLGWPK"
     gene            57699..58427
                     /gene="yaaA"
                     /locus_tag="VW006_000425"
     CDS             57699..58427
                     /gene="yaaA"
                     /locus_tag="VW006_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9379338.1"
                     /translation="MKILIPTAKEMNTDLPSIETTLLRSESQAVLDALALYSASQLES
                     FYKVSAEKAAEECQNIQALKRQTAQHYPALKLFDGLMYRHIKRDKLTEVEQAYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDEAVKQEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(58478..59962)
                     /locus_tag="VW006_000426"
     CDS             complement(58478..59962)
                     /locus_tag="VW006_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9379339.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
BASE COUNT        17536 a        11227 c        13725 g        17586 t
ORIGIN      
        1 ccaatctact atataaatga aaaccttagc tgtttggcca aggttcattt cttctatgca
       61 agttcttctc ttttcttatc tgggttccag atgaagcttg cgataaagct aaagatgatg
      121 gttaggatag cgaggataat agcaaggatg agggcaggga tgcggataaa ccaccagagt
      181 ggatttggtt ttttatcatt gtgccattgt ttcgtttctt cgtccagacg ttggaattct
      241 gtttgaatac gattggcgac tgtttcaatg ccttcatcat tcattttctt gatatctgag
      301 atatcgattg gatttccaaa gttcatatcg acacgttcac ggctaatcaa gcccttcaaa
      361 gtcatgggac cggtgtaggt aaccggcatg atacggacct tggccatttt ggcaatcagt
      421 gctgcgcccc ccttgacatc gtttgagtgg cggctaccac ttggaaacat gatgagagag
      481 cggtcacttt ttttgagaac gttgatagga tatttgatgg ctgaggcgct gggattttca
      541 cggtcgatgg gaaaggcgcc acacatacga atccaccaac caaagatacg gttggtaaag
      601 agttcttttt ttgccataaa gatgaactgt tttggcttgg tcgcaaaggc catataaaca
      661 ggatcccacc aggtacggtg aggcgcaact aaaatataat tttcatcttg attaggaatt
      721 ttatcagtat tatgatagtg agcattgcca ttgatggacc ataagagcaa tacaactaat
      781 ccacgcaaat aagtataaaa catgcgatct ccttcgattg ttttcttgtt attattatac
      841 cttatcaaag gagggctggc aaacttttcc cttgactaga tacatattta ggatgaaatt
      901 agaattctgt taaaaaaaat gatataatag aatttatgga taaaaataag attatgggat
      961 taacccaaag agaagtcaag gaaagacagg ctgagggttt ggtcaatgac tttaccgcat
     1021 cagccagtac cagcacttgg caaatcgtta aacgaaatgt ctttaccctt tttaacgctt
     1081 tgaactttgc cattgctttg gctcttgcct ttgtgcaggc ttggagcaat ctggtcttct
     1141 ttgctgttat ctgctttaac gctttttctg ggattgtgac cgagctacga gccaaacaca
     1201 tggtggacaa gctcaatctc atgaccaagg aaaaggtcaa aaccatccgt gatggtcagg
     1261 aagttgctct taatcctgaa gaattagtgc taggagatgt cattcgtttg tctgcaggag
     1321 agcagattcc tagtgatgcc ttggttttgg aaggctttgc ggaagtcaat gaagccatgt
     1381 taacgggaga aagtgatttg gtgcaaaagg aagttgacgg cttacttttg tcaggaagtt
     1441 tcctagccag tgggtcagtt ttatctcaag ttcaccatgt cggtgcagac aactatgctg
     1501 ccaaactcat gcttgaggct aagaccgtta aacccatcaa ctcccgtatc atgaaatcgc
     1561 tggacaagtt ggctggtttt actgggaaga ttatcattcc ctttggtctg gctctcttgc
     1621 tggaagcctt gcttttaaaa ggcctgcctc tcaagtcatc cgttgtaaac tcgtcgacag
     1681 ctcttttggg aatgttgcct aagggaattg cccttttgac cattacttcg ctcttgactg
     1741 cagtgattaa gttgggcttg aaaaaggtct tggtgcagga gatgtactct gttgagacct
     1801 tggcgcgcgt ggatatgctc tgtctggaca agacgggtac catcacccaa ggaaagatgc
     1861 aggtggaggc tgttcttccg ttgatggaaa cgtatggtga agaggctatt gccagcatct
     1921 tgactagcta catggcccat agtgaggata agaatccaac tgcccaagcc attcgccagc
     1981 gttttgtggg agatgttgct tatcctatga tttccaatct tcccttctcg agcgaccgca
     2041 agtggggggc tatggagtta gaaggcttgg ggacagtttt cttaggggcg cctgagatgt
     2101 tgctggattc tgaagtccca gaagccaggg aggccttgga gagaggatca cgtgtcttgg
     2161 tcttagctct cagtcaggag aaattagacc atcacaaacc acagaaacca tctgatattc
     2221 aggctctagc cttgctggaa atcttggact ccattcgaga gggagcagca gagacgctgg
     2281 actatctccg ttctcaggag gtaggactca agattatctc tggtgacaat ccagttacgg
     2341 tgtccagcat tgcccagaag gctggttttg cggactatca caactatgta gattgctcaa
     2401 aaatcaccga tgaggaattg atggccatgg cggaggagac agctattttc ggacgtgttt
     2461 cccctcatca aaagaaactc atcatccaaa cgttgaaaaa agcgggtcat acaacggcta
     2521 tgacagggga cggggttaat gatatcttgg cccttcgtga ggcggattgt tctatcgtga
     2581 tggcggaggg ggatccagca acccgtcaga ttgccaatct ggttctcttg aactcagact
     2641 ttaatgatgt tcctgagatt ctcttcgagg gtcgtcgcgt ggtcaataac attgcccaca
     2701 tcgccccgat tttcttgata aagaccatct attccttcct gttagcagtc atctgtattg
     2761 ccagtgcttt actaggtcgg tcagagtgga ttttgatttt ccccttcatt ccgatccaga
     2821 ttaccatgat tgaccagttt gtggaaggtt tcccaccatt cgttctgact tttgagcgaa
     2881 atatcaaacc tgttgagcag aatttcctca gaaaatccat gcttcgtgcc ctaccaagcg
     2941 ctctcatggt cgtctttagc gtcctgtttg tgaaaatatt tgggagtagc caaggttggt
     3001 ctgagttaga aatctcaact ctcctctatt atctcttggg gtcaattggt ttcttatccg
     3061 tatttagagc ctgcatgcca tttaccctat ggcgtgtcct cttgattgtt tggtcagtag
     3121 gaggtttcct agccacagct ctcttcccaa gaatccaaaa actgcttgaa atttcaacct
     3181 taacaggaca aacattacct gtttatggtg tcatgatgtt ggtctttacc gtgattttca
     3241 tcctgaccag tcgctatcaa gccagaaaat aaagaaaggc tgcaatctgt ggattgcagt
     3301 ttttttaggt acaagattgc cagtcgaaat gtggtataat aagaggtaat agagttttgg
     3361 aaagtgagag aagatgattt caaagagatt agaattggta gcttcctttg tgtcacaggg
     3421 ggctatttta ctagatgtgg gaagtgacca tgcttatctg cctatcgagt tggttgagag
     3481 aggccaaatc aaaagcgcta ttgcaggtga ggtggtggaa ggtccctatc agtctgcggt
     3541 taaaaatgtt gaggctcacg gcctaaagga gaaaatccaa gtccgtttag ccaatggctt
     3601 ggcagctttt gaagagactg accaagtgtc tgtcattacc attgctggca tgggtggtcg
     3661 tttgattgct aggattttag aagaaggttt ggggaagtta gctaatgtag agcgtttgat
     3721 cctccagccc aataatcgtg aagacgactt gcgtatctgg ctacaggatc atggattcca
     3781 gattgtagca gaaagcattt tagaagaagc tggaaagttt tatgagattt tggtggtgga
     3841 agcaggacaa atgaagctat cagccagtga tgttcgcttt ggtcccttct tgtccaaaga
     3901 agtcagtcca gtatttgtcc aaaaatggca aaaagaagct gagaagctag agttcgccct
     3961 cggacaaatc ccagaaaaaa atctggaaga acgtcaagtt ctagtagata agattcaagc
     4021 tatcaaggag gtgctccatg ttagcaagtg aagtgatcca agcttatgaa gccttttgcc
     4081 ctcaggaatt ttctatggag ggagacagcc gtggtctgca aattggcact ttagacaagg
     4141 gtatccaaag ggtcatggtt gccctcgata ttcgtgaaga gacggtggca gaggctattg
     4201 aaaagggtgt ggacttgatt atcgtcaagc acgcgcctat ctttcgtcct atcaaggact
     4261 tgcttgccag ccgtccgcaa aatcagattt acatcgacct gattaagcat gatatcgcag
     4321 tttatgtcag ccataccaat attgatatcg ttgaaaatgg gctcaatgac tggttttgtc
     4381 agatgctagg aatcgaggag acgacttatc tgcaggaaac aggtccagaa cgtggaattg
     4441 gacgtattgg gaatattcag cctcagacat tttgggaatt ggcccaacag gtcaagcaag
     4501 tctttgacct agatagcctt cgaatggtgc attatcaaga gaatgatttg cagaagccta
     4561 tttcaagagt agcaatttgt ggtggaagcg ggcagtcttt ctataaggat gctttggcaa
     4621 agggggcaga tgtctatatc actggtgata tctactacca tactgctcag gatatgctgt
     4681 ctgatggctt gttagcattg gatccaggtc actatatcga agtgcttttt gtggaaaaaa
     4741 tcgcagcact ccttactcaa tggaaagaga aaaaaggctg ggaactagag attttaccta
     4801 gtcaagcatc gaccaatcct ttccgtcata tctagttaga aagtgaagac aatgaaaaaa
     4861 gttgccatta ttggagcagg aattgtggga gcaacagctg cctactacct ctcgagagaa
     4921 agtgacctag aggtgaccgt ttttgaccat gagcaaggtc aagccaccaa ggccgcagca
     4981 ggaattatca gtccttggtt ttccaaacgc cgtaataaag cctggtacaa gatggcgcgc
     5041 ttgggggctg atttttatgt ggatttatta gctgatttag agaaatcagg acaagaaatc
     5101 gacttttacc agcgttcggg agtctttctc ttgaaaaagg atgaatccaa gttggaagaa
     5161 ctctatcaac tggctctcca gcgcagagaa gaatctcccc tgataggcca gttagccatt
     5221 ctggatcaag catcagctaa tgaattattc cctggcttgc agggattcga caacttgctc
     5281 tatgcttctg gtggagcgag agtagatggc caactcttgg taactcgtct gctagaagcc
     5341 agtcatgtca agttggtcaa agaaaaagtg actctgacac tcttatcatc aggctaccag
     5401 attggcgaag aggtgtttga tcaggttatt ttggcaacgg gagcttggtt gggggacatg
     5461 ttagaacctt taggttatga agtggatgtc cgtcctcaaa aaggacaact acgagattat
     5521 cagcttgccc aagacatgga agattaccct gttgtcatgc cagaagggga gtgggatttg
     5581 attccctttg caggtgggaa attatccttg ggcgctaccc atgaaaatga catgggattt
     5641 gatttgacgg tagatgaacc cttgctccaa caaatggagg aggcggcctt gcctcattac
     5701 ccagttttag ctagagtgac ttcaagggct gagcgtgtgg gaatccgtgc ctataccagt
     5761 gatttctctc ccttctttgg gcaggtgcca gaattggcag gtgtctatgc agccagtgga
     5821 ctaggttcat caggcctcac aactggtcct atcattggtt accatctagc ccaactgatc
     5881 caagacaagg agttgacctt ggaccctcta aattacccaa ttgaaaacta tgtcaaacga
     5941 gtaaaaagcg aataatactc aatgaaaatc aaagaacaaa ctaggtaact agccgcgggt
     6001 tactcaaagc actgctttga ggttgtggat agaactgacg aagtcagctc aaaacactgt
     6061 tttgaggttg tggatagaac tgacgaagtc agtaaccata cccatggcaa ggcgacgttg
     6121 acgtggtttg aattggattt tcgaagagta taagcatttt actgaaattt tatcaggtag
     6181 tttaggatgg caaatgacat tccctgtcaa aaatggtaaa ataagaaaaa ataatctgag
     6241 aatcgaggaa aaaagatgca agaaaagatt ttggtaactg gtggtgccgg ttttattgga
     6301 acccacactg ttattgaatt gatccaagca ggccatcaag ttgttgtggt ggataatctt
     6361 gtcaatagca atcgtaagag tttagaagtt gttgaaagaa tcacaggagt tgagattcct
     6421 ttctatgagg cagatattcg tgacacagct accctcagag atattttcaa gcaggaagaa
     6481 ccgactggtg tcattcactt tgctggtttg aaggctgttg gcgaatcaac acgtatccct
     6541 cttgcctact atgacaacaa tatcgctgga actgtcagcc ttttgaaagc catggaagaa
     6601 aacaactgta aaaacatcat cttcagttct tctgcgacag tttacgggga tccgcacaca
     6661 gtgcccatct tggaagattt cccactttca gtgaccaacc catacggtcg tactaagctc
     6721 atgctagagg aaattttgac tgatatttac aaagcagact cagaatggaa tgttgtcttg
     6781 cttcgttact ttaacccaat cggagcccat gagagtggtg atttgggaga aaatccaaac
     6841 ggtattccaa acaatctctt gccatatgtg actcaagtag ccgttggaaa attagagcaa
     6901 gtgcaagtgt ttggagacga ttacgatacg gaagatggaa caggtgttcg tgactatatc
     6961 cacgttgtcg atttggctaa gggtcacgtt gcagctttga aaaaaatcca aaaaggttca
     7021 ggactaaacg tttataacct tggaactggt aaaggttact cagttcttga aattatccaa
     7081 aacatggaaa aagcggtggg ccgtcctatt ccttaccgca tcgtagaacg tcgcccaggt
     7141 gatatcgctg cctgctactc agacccggca aaagctaaag cagaactcgg ttgggaagca
     7201 gaactcgaca tcacccaaat gtgtgaagac gcatggcgtt ggcaaagcaa gcatccaaat
     7261 ggatttgaag actaagatga tgatttcaat catcgtccct tgtttaaacg aagaggaagt
     7321 acttcctctt ttttatcagg ctctggaagc tttacttcca gatttggaaa cagaaatcga
     7381 gtatgtcttt gtcgatgatg gatcaagtga tgggaccttg gaactcttaa aggcctatcg
     7441 ggagcaaaat ccggcagtcc attatatttc tttctctcga aattttggca aagaagcagc
     7501 cctttatgca ggcttgcaat atgcgacagg agatttggtg gtggtgatgg atgcagacct
     7561 ccaagatcct cctagtatgt tgtttgagat gaaaaatgta ctagacaaaa atgtagactt
     7621 ggactgcgtt gggacacgga gaactagtcg ggagggagaa cccttctttc gcagtttctg
     7681 tgctgttctc ttttatcgcc tcatgcaaaa aatcagccca gtagctctgc cgtcgggtgt
     7741 ccgtgatttt cgtatgatga gaaggtctgt agtcgatgcc attttaagct tgaccgagtc
     7801 caatcgtttt tcaaaggggc tcttcgcctg ggtgggtttc aaaacctact atttggacta
     7861 tccaaatgtc gaaaggcagg ctggcaagac cagttggagt tttaggcaac tcttttttta
     7921 ctccattgaa gggattgtta atttttcaga tttccctttg actatagcct ttgtagctgg
     7981 tctcctatct tgttttcttt ctctgctgat gacctttttt gttgtggttc ggaccctcat
     8041 tttgggcaat ccgacatctg gttggacctc tctgatggct gttattctct ttcttggagg
     8101 cattcaactc ttgaccattg ggattctcgg taagtatatc agtaagattt atttagagac
     8161 taaaaaaaga ccactttatc ttatcaaaga aaaaagtgac cttcctgatt ttacagaaaa
     8221 aataaagtga aaagactata attttacatg gaaatgtgct aaactagagg gagtggatta
     8281 tcgccattga tttaggcttc ctggtttcca atcagggcgc gagttgggca atttttaaca
     8341 gcctctaaga catcctgact aggagaaatt tctttttcca gttggtcagg gtcatcgtaa
     8401 aaacgcacga ttccattatc gtggtaatca aataaatcag aataagtttg gcaaagtcca
     8461 caggcgatac atcgttcagg tataagtgtg attttcatgt tcatatttat attgtaataa
     8521 gaaaagtaaa aaaaaacaag gagtaaggta tggcaaaaga accgtggcaa gaagatattt
     8581 atgatcaaga agaatcaaga gcagggcgtc ggcatcgaaa ccacggaggg gctgatagga
     8641 tggctaatcg tattttgacg atcctagcta gtattttctt tgtaattgtg gtggtgatgg
     8701 tcatcgttct catctatcta tcatcggggg ggagtaatcg cacagcagcc ttaaaagact
     8761 ttcatgattc tgatgcaagt gtagtacaaa tctcatcttc aagcagttct cagcctgagc
     8821 agagttcaga gccagaatct acttctagta gttcagaaga agctgctaat cctgaaggaa
     8881 cgattaaagt tctcgcagga gaaggggaag cagctattgc cgctcgtgca ggaatctcca
     8941 ttgctcagtt agaggccttg aatcctgggc acatggctac aggatcttgg tttgctaatc
     9001 caggtgatgt tataaaaata aaataggagc cgatatgaaa acaattcaaa ttgctattga
     9061 tggtcctgct tccagcggta agagtacggt cgcaaagatt attgctaagg attttgggtt
     9121 cacctacctt gatacaggag ctatgtatcg tgcagcgacc tatatggctc ttaagaacca
     9181 attaggagtt gaagaagtcg aagcccttct agccttgttg gaccagcatc caatcagctt
     9241 tggacgttca gaaactggag accagcttgt ttttgtagga gatgtggata ttacccatcc
     9301 tatccgtgaa aatgaagtga ccaatcatgt ttctgctatt gcagcaattc ctgaagtgcg
     9361 tgagaaactg gtttctctcc aacaagaaat tgcccagcaa ggcgggattg tcatggatgg
     9421 tcgcgatatt ggaactgttg tattgccaca agcagaattg aaaattttcc tagtagcttc
     9481 tgttgatgag agagcagagc gtcgttacaa ggaaaatatt gccaagggaa ttgaaacaga
     9541 ccttgaaacc ctaaaaaagg aaattgctgc gcgtgactac aaggatagtc atcgtgagac
     9601 ttctcctctc aaacaagcag aggatgctgt ctaccttgat acaactggtt tgaacattca
     9661 agaagtagtt gaaaaaatca aagcagaagc tgaaaaaaga atgtaaatgt agaaaagccg
     9721 atagattgat atcggttttt ttctaaattt gtcaaaatgc tgattttagg gtataatagt
     9781 tgctgttcga gaaattttga ttttcaaaga atagtgaatg atgataagga gaaaccatga
     9841 acaatttacc aaattgtcca aaatgtaact cagagtatgt ctacgaagac ggtgccctac
     9901 tggtttgccc agagtgtgct catgagtgga atcctgctga agttgcagaa gtagaagaag
     9961 gtcttgtcgc tatcgatgcc aacggaaata aattgactga tggtgataca gtaactctca
    10021 tcaaggactt gaaagtaaaa ggtgcgccaa aagatttgaa acaagggacg cgcgtgaaaa
    10081 atatccgcat cgtagaaggc gatcacaata tcgactgtaa gattgatggt tttggtgcca
    10141 tgaaacttaa atcagagttt gtgaggaaga tttaagcatg tcaaaacact ataaacttgt
    10201 attttatagc cgtatcttct tgtttctagc ggcttttacg ggagtttatc ttgaaattac
    10261 taggcatggt ggttttggga tgcttctcta ttacacggtt ctgtccaacc tcttggtaac
    10321 tatttttacc ctttatcttc taaaggttat gagccgtgta ggtgaaaatt ggcaaaggcc
    10381 aagtctcttg cgcttaaaag gtggggtcac catgagtatc atgattacct gtgtgattta
    10441 ccatttcctc ttggcgccca ttgcgactaa tttctatacc ctagaaaatt tcctttgcca
    10501 ctatatcgtt cccatctggt ttttagcgga taccctcttt tttgacaaac agggtcaata
    10561 caagatttgg gatccagcag tgtggaccat tttacccttt ctgtatatga tgtttgctct
    10621 ttttaatggc ttggttctaa aactcaatat tccaaatgcc aaggataatc ctttccctta
    10681 cttctttttg aatgtgaaca agggttggaa tgttgtgttt aagtggtgtc tgattatctt
    10741 tgttgcctat atggtagcag gatttatttt ctactttatc aagcaaatca agagaaagtc
    10801 atcctaatac tcttcgaaaa tctcttcaaa ccacgttagt ttccatctgc aacctcaaaa
    10861 cactgttttg aacaacctgc ggctagcttt ctagtttgct ctttgatttt cattgagtat
    10921 gagatacaag ggattcattt tcgaatttaa gaaggagtct acaaaccaat gaggcttctt
    10981 cttttcttgc ttatttgata actatcaaaa aagtagaaga ttaagagaaa tatagaaaga
    11041 gatttcatga aacaattttt agaacgggcc agcattttgg ctctctccct cgttttgatt
    11101 acctcctttt ccatttcgag tgccctacca gccatgtttg actattatca gggttattct
    11161 aaggaacaaa ttgagctctt ggtgagcttg ccttcctttg gaatcatgat gatgttactg
    11221 ctaaatggtt tcttagaaaa aatatttcct gagcgcttac agattagttt gggcttgctg
    11281 attttatcat tgagcggtac agctcccttc tggtaccaag cctatccctt tgtctttgga
    11341 acacggcttc tctttggttt gggtcttggg atgatcaatg ccaaggccat ttctattatc
    11401 agtgaacgct accaaggaaa aaggcgaatt cagatgttag ggctacgcgc ttctgcagag
    11461 gtcgttggag cttctctcat taccttggcc gtcggtcagt tgttggcctt tggttggaca
    11521 gctatctttc tagcctatag tgctggattt ttggtgatgc ccctttatct gctctttgtc
    11581 ccttatggaa aatcaaagaa agaagtcaag aaaagagcga aggaagcaag tcgtttaact
    11641 cgagaaatga aaggcttgat ttttacctta gctatcgaag cggcagttgt agtttgtacc
    11701 aatacagcta ttaccatccg tattccaagt ttgatggtgg aaagaggatt gggggatgcc
    11761 cagttatcta gttttgttct tagtatcatg cagttgatcg ggattgtggc tggggtgagt
    11821 ttttctttct tgatttctat ctttaaagag aaactgctcc tctggtctgg tattaccttt
    11881 ggcttggggc aaatcgtgat tgccttgtct tcatccttgt gggtggtagt agcaggaagt
    11941 gttctggctg gatttgccta tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa
    12001 cgaattccag ctaaactcct caatcaagca acttcatttg ctgtattagg ctgtagtttc
    12061 ggagccttta cgaccccatt tgttctaggt gcaattggct tactaactca caatgggatg
    12121 ttggtcttta gtatcttagg aggttggttg attgtaatct ctatctttgt catgtaccta
    12181 cttcagaaga gagcttagga ttgattccta agtttttctt ttgatactca atgaaaatca
    12241 aagagcatag ttgattgagt ttggaatagt acgctgtagc tgctaaaata tttctagaaa
    12301 ttaatttgac ttttctaata gagttattca tatcttattt caatctacta tagaaagcta
    12361 gccgcaggtt gctcaaaata gtgttttgag gttgcagatg gaaactgacg tggtttgaag
    12421 agattttcga agagtatgag atttttatac ccatacaata tttgttttag gtccttgttt
    12481 acagactaat ttcttgataa aatagaggtt atgacgagat ataaagcaac tatttcctat
    12541 gatggttatg cctttgctgg ctttcagcgc cagtctcatg cgcgtagcgt tcaggaagaa
    12601 attgaaaaaa ccttgaccag attaaataaa gggcaaacca ttactgttca cggtgctggt
    12661 aggacagata gtggggttca tgccctggga caggtcattc attttgacct gccttatcag
    12721 atggatgagg agaaactccg ttttgccttg gatacccagt ctcctgaaga tattgatgtg
    12781 atttcgattg agcttgtggc agatgatttt cattgccgtt atgccaaaca tagcaagacc
    12841 tatgagttta ttgtggatag aggacgtccc aaaaatccta tgcgccgtca ctatgccacc
    12901 cactttccct acccactcga tgtggaacga atgcagattg caatcaaaaa gctagaggga
    12961 acccatgatt ttaccggttt tacagcctct gggactagtg tagaggataa ggttcgcacc
    13021 atcacagaag ctagtttaat agtcgatgag acaggacaat ttttgacctt taccttttca
    13081 ggaaatggtt tcttgtataa acagattcgc aatatggtgg ggactcttct caaaatcggt
    13141 aacaaccgca tgccagtaga gcagattgat ctcatcttgg agaagaagga caggcaactt
    13201 gcaggtccca ctgcagcacc aaatggtttg tatttaaagg agattcgtta tgaagaataa
    13261 tcgtatttta gcactttctg gaaatgatat ttttagtggt ggtggactgt cagctgattt
    13321 ggctacctat accttgaacg gcttgcatgg gtttgtagca gtgacttgtt tgacagcctt
    13381 gacagaaaaa ggatttgaag tctttccaac tgatgatacc atttttcaac atgaattaga
    13441 tagcttacgt gatgtggaat ttgggggaat taagattggt cttctcccta ctgtcagtgt
    13501 ggctgagaag gccttggact ttatcaaaca acgcccagga gtacctgtgg tgttggatcc
    13561 tgtcttggtc tgcaaggaaa cgcatgatgt agctgtcagt gagctctgcc aagagttgat
    13621 tcgctttttc ccttatgtca gtgtgattac gcctaatctc ccagaagcag aattattatc
    13681 cggtcaggaa attaaaacct tggaagacat gaaaactgca gcgcagaaat tgcatgattt
    13741 aggagcgcca gcagtcatta tcaagggagg caatcgtctt agtcaggaca aggctgtgga
    13801 tgtcttttat gatggacaga cctttactat cctagaaaat ccagttatcc aaggccaaaa
    13861 tgctggtgca ggttgtacct ttgcctctag cattgccagt cacttgatta aaggtgataa
    13921 acttttgcca gcagtagaaa gctctaaggc tttcgtttat cgtgctattg cacaagcaga
    13981 tcagtatgga gtaagacaat atgaagcaaa caaaaacaac taaaatcgcc cttgtatccc
    14041 tattaaccgc cctttctgtg gttctaggtt atttcttaaa aatcccaaca cctacaggaa
    14101 ttctaactct tttagatgct ggtgtcttct ttgcggcctt ttactttggt agtcgtgaag
    14161 gagcggtagt cggaggacta gcaagtttct tgattgacct cttatcaggc taccctcagt
    14221 ggatgttctt tagcttggtc aaccatggct tgcagggatt tttcgcagga tttaaaggaa
    14281 aaagtcagtg gttaggcctt attttagcaa ctattgccat ggtaggaggc tacgccttgg
    14341 gttctacttt gatgaatggc tgggcagcag ccctcccaga aattctacca aatttcatgc
    14401 aaaatatggt agggatgatt gtaggattta ttcttagtca aagtatcaag aagattaagt
    14461 aatactcttc gaaaatctct tcaaaccacg tcagcttcca tctgcaacct caaaacactg
    14521 ttttgagcag cctgaggcta gctttctagt ttgcactttg attttcattg agtataaaga
    14581 ggttgagtaa aaagtatttc aaataagaaa aacgcatagt atcaggtgtt gaatgaactg
    14641 ccccccaaaa gttagacaga aaaaatctaa cttttggggt gttttattat gaaactaact
    14701 tatgatgata aagttcagat ctatgaactt agaaaacaag gatatagctt agagaagctt
    14761 tcaaataaat ttgggataaa caattctaat cttaggtaca tgattaaatt gattgatcgt
    14821 tacggaatag agttcgtcaa aaaagggaaa aatcgttact attctcctga tttaaaacaa
    14881 gaaatgattc ataaagtctg acatgaaggc tagactaaag atagagtttc tcttgaatac
    14941 ggtctcccaa gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat
    15001 actattgttg agaaaacaag agggagagta cctaaaatgg gacgtaagcc aaaaaagaga
    15061 cctgaagaga ggacagaatt agaacgtctt caagcagaaa atgagtacct gagagcggag
    15121 aatgccatcc taaaaaagtt aagagaactc cgattgaagg aggaaaaaga gaaagacaga
    15181 aattgtttaa gaattaatga ctgagttttc gttagatctt cttctaaaag ccattaaact
    15241 agctcgttcg acctactact atcacttgaa acagctagac aaaacagata aggaccaaga
    15301 gcttaaagct gaaattcaat ccatttttat cgaacacaag ggaaattatg cttatcgtcg
    15361 aattcattta gaactaagaa atcgtggtta tctggtaaat cataaaagag ttcaaggctt
    15421 gatgaaagta ctcaatttac aagctaaaat gcgacagaaa cgaaaatatt cttctcataa
    15481 aggagacgtt ggtaagaaag cagagaatct cattcaaggc caatttgaag gctctaaaac
    15541 aatggaacag tgctacacag atgtgactga atttgccatt ccagcaagta ctcaaaagct
    15601 ttacttatca ccagttttag atggctttaa tagcgaaatt atcgcctata atctttcaac
    15661 ttcacccaac ttagaacaag tacaaacaat gttggaacag gcattcacag agaagcacta
    15721 cgagaatacg attctccata gtgaccaagg ctggcaatat caacacgatt cttatcatca
    15781 gtttcttgag ggtaagggaa ttcaagcatc catgtcacgc aagggaaaca gccaagacaa
    15841 cggtatgatg gagtccttct ttgggattct gaaatcggaa atgttttacg gttatgagaa
    15901 gtcgtttcag tcgcttaagc aattggaaca agctattgta gactatattg attactacaa
    15961 caataaacga attaaggtaa aactaaaagg acttagtcct gtgcaataca gaactaaatc
    16021 cttcggataa attaattgtc taactgtttg ggggcagtac attttaacac ttgatactat
    16081 gcgttttatt gtgggaagat tttcctcatt ttctagtgaa attgaacttt ttctaacatc
    16141 ttttgataga tgacttctta atcaattatc atttccattg ttgtttcttg gggtttcatc
    16201 ccttgtcttt gataaaagcg gagagcaccc tcgttatcat tccagacact tagggttaga
    16261 ttattgcagc caatttcttt agcataatag cagaaaaatg acaataaaat agcccctaga
    16321 tattttgaaa ggaaagcata ggggctatat agtttaatca aaataaagca aatctttgct
    16381 ggtgcttgta aagaggtcga agccatcctt ggtaacaaca ccgcagtctt caatacgaac
    16441 accgacttta ccagggatat agatacctgg ttcaacagag aagcacatgc cttcttcgat
    16501 gaccatgtcg tttccttcca tgatagatgg gaattcatgg acatccatac cgataccatg
    16561 cccgagacgg tggttgaagt actcaccata accagctttt tcgatgacct cacgggcagc
    16621 gcggtccact tcatgagcag tcacacctgg cttgataaag tcaagagcag cttgttgggc
    16681 ttcaagagtc aagttgtaaa tatctttctt gaattggtct ggtttgccga cagcgactgt
    16741 acgagtcata tctgacgcat agccattgac cagaacaccc aggtcaaaga ggagaagagc
    16801 atcattttca accttattag ctgctggaat gccgtgtgga ttcgcagcat tatcaccagt
    16861 caagaccatg gtatcaaagc tcatttcata accttcacgt ttcatggcaa agtcgatttg
    16921 tgcgatgata tctgtctcag tcttatcaag agaaatattg tcaaaaccaa catgaacagc
    16981 cttgtcagca taaagacctg caaccatcat tttttgcact tcatcagctg atttgatgag
    17041 gcgcatgcgt tggatacgag gagtgaggtt gtcaaactca gcagtctcaa aaactgtttt
    17101 caaaccatgg tatttggtca agatgagatt gtcaaactca acagcgacac gtttgaagtc
    17161 aagttgtgga agagcatgtt tgattttttg ccatggattt tcagaatcga catagcccac
    17221 tactgggaag gaaacggtgc tacttgcacg ttctacttca agagctggga caaagaggag
    17281 aggttcctga tccgctagga caaagaggaa catttggcgt tcatggggat cactgtaaaa
    17341 accagtgagg taattgattg tgacggggtc agatacgaca gcgacgtcta gtttttctga
    17401 ttcaagatat gttaggattt gttgtaattt agacatatgc taccttcttt ctacccctct
    17461 attttggcaa aaaatgagag aaaatgcaag ggagaagggt aaaagaaaag acaaaaaact
    17521 ccgacaagat aacggagttc tttgatatca ttttcctttg tttaagaaaa gtcagccttg
    17581 cttttgacgt cactgtactc ttcagcgatt tcttggctga gaatgtcctc ataggcaggg
    17641 agttggatgt cctgaccata tttcttcttg agggcagtag taactaggcg ataagccaga
    17701 ttggcattac gagagaggat aggcccgtgg aagtaggaac caaagacatt cttataatga
    17761 accccttcac cgaccttttc ttcgttgttt ccatttccat agacaacctg tcccagcggt
    17821 ttttggtcat cagagaggaa ggtacgacct tggtgatttt caaatccata gtaggtttca
    17881 tcgaaatctt cattgtgaat cttgatgtca ccgataaaac ggttattggt ctggttgagc
    17941 gtgtagtgtc ccatgacccc tagcccttcg atacgttttc ctgaagcttc aacataatat
    18001 tgacccaata gttggaaacc accgcagata gccagaacta caccgtcgtt ttggatgtag
    18061 ttgtcaatgc tctctttttt agcaggtagg tcgtctgcaa tgatactttg ttcaaagtct
    18121 tgaccaccac cgaaaaaggc gatgtcgtag tgattttcat caaagtcatc atggagagaa
    18181 acgatgtcaa cggtcacatg ggctcccagt ttttcagcca catacttgag catgaggatg
    18241 tttccattgt ccccgtaggt attcatgaga tttccgtaga ggtgggcaat gttgagctga
    18301 taggggtaat tgctatcttt tgaggaaagt gaagtataaa ccattagttc atctcctttc
    18361 taacaatctg acgactagcc agcagttcac gaaattccag catggcagta taagttgcca
    18421 gaatataggc atgcttgcag tcttgattct caatggtctt gagaacttgc tccagattac
    18481 tcgtttcagt gattttctca gctggatagc cagtcactcg gaggcgacga gcgatttcag
    18541 aatgacgaac accgccagcg ttgatttcag gaatgtccat gtcagtgatt tgctcaaagt
    18601 ctgcatccca gatccagcta gtgtcaattc catctgcata gttggcatta aggaggacag
    18661 ataggctaaa tggataaggt gctagtttga tcatttcgat agcttgggtt gcaccgactg
    18721 gatttttaat caagacaagg gtacattcct tgtcaccgat atgaaaggtt tcttggcgtc
    18781 caaagacagc acggctcttg tcaaatccct gtttgatgag ttgggaatct gcgccgagga
    18841 aacgggcgat ggccacagca gctagggcgt tatagatatt atagagcccg ccgatttgga
    18901 taccgtattc ttggccgtct atgacaaagc gagagcgatt gttggtcaac tcaaccagtt
    18961 ttgtcaaacg atagtcgaga tcaggacgtt tacatccaca accttcacag atataggcac
    19021 ccaagtttgc ataggtatta tgctcatatt tgaggatgcc ttggcagtca ggacagagaa
    19081 tcccttcggt attgtagtga gcaagttggg ctggtccttt ttccaagtca aaaccaaaat
    19141 actctatagg gtttggaata gttggcttgt agaaaagtgg actgtctccg ttaaggagaa
    19201 cagtagcagt tggaactttt cgaatggcat ccaatatcat gttataggta gtatagattt
    19261 caccgaaacg gtccatctgg tcacggaaga tattagtaat gacaaaaaga ctaggctgga
    19321 tatagtcaca gatacgagat agactggctt cgtcaatttc gaggacggca atatttttcc
    19381 cagtttttga agatttggct gttaggaagg ttgttgcaat ccctgtaatc atgttggcac
    19441 cgcttgggtt ggttagaact tgaccataaa cctcttttaa aatgccgaca gtgagggcag
    19501 ttgtcagggt ttttccattt gttccagtga caacgacaat ctcgtagttc ttagctaggc
    19561 tttgtaaaat atctttatca aattgaaggg cgactttccc tgggagcgta cttccacgtc
    19621 caagacggct taaaacgaag tgggaagaac gcccagcaag aaggcccaaa gtagttttta
    19681 agttcatgtt tctattatat cacaaaagag ggaaaagaca gatttggtgt ttcgtagaag
    19741 taaaaacagc ccaaagaggg ctgtcagtta agatgagatt tcaacttaaa tcgcaaacaa
    19801 gtcattcaag ttctcagcca aggttgggtg agtgaagatt tgttttgtga agtaagtgta
    19861 aggaatcttg ttgtccatag caacagtaat gatgttgatg atttcttgag aaccttctga
    19921 gaagatgctt gcaccaagaa tttcttttgt ttcagtatta acaacagctt tgaaagctcc
    19981 gcgaaggtct ccatttacgt gaccacgagg catggctgca acagggattt ctttcactgc
    20041 gtatggaagt ttcaaatcag ctgcttggct ttcagtcaaa ccaacttgtg aaagtgcagg
    20101 tgtgatgaac atagtatttg gcacattgag acggtcctca agtgtgtagc tgccatctcc
    20161 agcaaggtag ctgtaaacaa cacggaagtc atcaagtgaa atgtaagtaa attgaaggcc
    20221 accgttgaca tctccaactg caaagacacc aggaacgttt gtttgacagt gtttgtctac
    20281 tttaatagca ccacgttcag ttagttcaat atctgtattt tcaagttgaa gtggttctac
    20341 atttggtttg cgtccagttg cgtagagaag ggcgtcgaaa cggtaagttt cgtcttcagt
    20401 tacgacaagc acttggtcac catcgttttt gatttcagta gtatggatat tttgaagcaa
    20461 ttcaatgcca tcttcttcca tgtattgttt agcaagagct gcgatggaag gttctgcacg
    20521 aggtaggaat gtatccaagg catctaggac tgtgaccttg cttccaagtt tgttgtaaag
    20581 gccggcaaat tcaagaccga tatttccgcc accaaggatt ccaagttttt caggtaattt
    20641 gtccaagctt tggatacctg ttgagtcaaa gatgtttttg cttgtagcaa gtccaggaat
    20701 tggcaagacg tttgaaacag caccagtgtt gatgacgatt gtttcagcag tcagttcttt
    20761 cttttcatca ccagcttgga tttcgatgac tttatttgaa aggaagtgag cttccgcatc
    20821 aaagatatct acgcctgtac cagcaacagt cgcatagttt ttaccgttga ggcgaccagt
    20881 gatcgtgttt ttagtagcaa tcacttcttc aaaagacaag tccttttcag cagcaactag
    20941 caaggtttta gttgggatac aaccaatgtt gatacaagtt ccaccgtaca tagccttgct
    21001 gcgttcaacg agggcaactt ttttgccagc tgaagccaat ttacctgcta gtgttttacc
    21061 agctttacca aatccaataa caattaaatc gtatgttaac atttatcttc ctcctattcg
    21121 aatttcattc tagcacaaga aaaaaacttt tgcacaattc tgctacggaa aagacagaaa
    21181 aagataaaaa cgctttccag acaaaaaact agtaattttt tgacaagctt gataaactaa
    21241 aatcctatct atttttatat aagaaagaat aaatgttcga aaataggagg ttttatgaaa
    21301 tcgaatcgtt atattattgc ctttgctggg gtcattttac acttaatgct gggttcgact
    21361 tatgcttgga gtgtttatcg taaccctatt attgaaaaaa cgggatggga tcaggcttct
    21421 gttgccttcg cctttagtct agcaatcttt tgtttgggct tatcggctgc atttatgggg
    21481 cgtttggtag aaaaatttgg tccgaaagtc atggggagtc tatctgcttt tctatacgca
    21541 ggtggaaata tcttaacagg atttgcaata gaccgtcagg aactgtggtt gttgtatcta
    21601 gcttatggca ttttaggtgg gcttggtttg ggagcaggct atattacccc tgtgtcaacg
    21661 attataaaat ggtttcctga taaacgtggt ctcgcaacag gtttagcgat tatggggttt
    21721 ggttttgctt ctttattgac tagtcccata gcgcaacacc tcatcgcagg ggtagggatt
    21781 gtagaaactt tttatatttt aggagcaagt tactttatta tcatgctcct agcttcacaa
    21841 ttcattaagc gtccaaatga gcaagagctt gcaattttat cttcttcagg gaaagaaaaa
    21901 acagcctctt tgacgcaagg aatggctgca aatcaggctc taaaaagcaa tcggttttat
    21961 atgctttgga ttattttctt tatcaacata gcttgtggtt taggcttaat ttcagcggca
    22021 tcgccaatgg cacaggagat ggctggcttg tctacaagtc atgcagcagt aatggtgggt
    22081 gttttgggga ttttcaatgg atttggtcgc ttgctctggg cgagtttgtc tgactatatc
    22141 ggtcgccctc taacctttag tatattactg cttgtcaatc ttttcttttc tctctcactt
    22201 tggctcttta cagattccgt tttatttgta gttgctatgt ctattttgat gacttgctat
    22261 ggagctggtt tttctttgat tccagcttat ctcagtgata tttttggaac caaggaattg
    22321 gccgctctgc atggttatat tttaacagct tgggcaatgg ctggtttagc gggacctatt
    22381 ttattagcag agacttataa aatggctcat tcgtacacac aaaccttgtt cgtttttctc
    22441 attttataca gtatcgcctt ggctttgtct tattatctag gtcgttcaat caaaaaagaa
    22501 agtcaaaaac cgcttacatg attttctgta gaaatggggc tatcttttgc tctggtcttg
    22561 tgttatacta gataggttgc aaagaaaaca gtacttttct tttgtggaaa agaagcaaca
    22621 cgattttata tctagttata tgaaaatacg tcataaaaag aaaagtataa actaagccct
    22681 atagggtggc ttcgcaccac ctttagaaag aagaataacg tgaaatttaa tgaattaaac
    22741 ttgtctgctg atttgctagc agaaattgaa aaagctggtt ttgtagaagc tagtcctatc
    22801 caagaacaaa ctattccttt ggcccttgaa ggcaaggatg ttatcggtca agctcagact
    22861 ggtacaggaa aaactgcagc ctttggcttg cctacccttg aaaaaatccg tatagaagaa
    22921 gcgactattc aagccttggt catcgctcca actcgtgaac tagctgtcca aagtcaagaa
    22981 gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc gttcagtata tggcggatca
    23041 agcattgaaa aacaaattaa ggctcttaaa tctggtgccc atatcgtggt gggaactcca
    23101 ggtcgcctct tggacttgat taaacgcaag gccttgaaat tacaagacat tgaaaccctt
    23161 atccttgacg aagcggatga aatgcttaac atgggcttcc ttgaagacat cgaagccatt
    23221 atttcccgtg tacctgagaa ccgtcaaact ttgcttttct cagcaactat gccagatgcc
    23281 atcaaacgta tcggtgttca gtttatgaaa gcccctgaac atgtcaagat tgcggctaag
    23341 gaattgacaa cagaattggt tgaccagtac tatatccgtg ttaaggaaca agaaaaattt
    23401 gacaccatga ctcgtctcat ggatgtggca caaccagaac tcgctattgt atttggtcgt
    23461 accaaacgcc gtgtggatga attgactcgt ggtttgaaaa ttcgtggctt ccgtgcagaa
    23521 ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg tccttcgtga ctttaaaaat
    23581 ggcaatcttg atgttttggt tgcgacagac gttgcagcgc gtggtttgga tatttcaggt
    23641 gtgacccatg tctacaacta cgatattcca caagatcctg agagttatgt tcaccgtatc
    23701 ggtcgtacag gtcgtgctgg taagtcaggt caatctatta cttttgttgc tccaaacgaa
    23761 atgggttacc ttcaaatcat tgaaaacttg actaagaaac gcatgaaagg tctcaaacct
    23821 gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag ctctcaagaa aatcgaacgt
    23881 gattttgcag atgaaaccat tcgtgccaac tttgagaaat ttggtaagga tgctcgcaaa
    23941 ttggctgctg agtttactcc agaagaattg gcaatgtata tcttgagtct gacagtccaa
    24001 gacccagata gccttccaga agtggagatt gcacgtgaaa aaccactacc gtttaaacca
    24061 tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg gaggccgtcg tggggacgac
    24121 cgtcgagagc gtgatcgtcg tggcaatggt cgccgtgatg agttcaaaaa aggaagtcgt
    24181 ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg ataataaaaa accacgcaat
    24241 actttaagcg aaaagcaaac aggctttgtt attcgtaaca aaggtgataa ataagaaaaa
    24301 gcggttcaaa atgaatcgct tttttatata aggagaccga atgtgaaaaa gacaaataaa
    24361 attattatat tatcttttat aatattatga tacacaaaaa taggcgactt gtcaatagaa
    24421 aaggaacaat ttaattaaaa atattctggt ttttaattaa attgtaagtc aatgcaagtc
    24481 aataaggaat tttcagataa ttattgaaaa agagacgttt ttatgttata atgatagcga
    24541 ttaaattcga ggtgaaaaat ggcacattta ttagaaaaaa ctagaaaaat tacatcaatt
    24601 ttgaaacgct cagaggagca gttgcaggat gagctccctt acaacgctat tacgcgtcag
    24661 ttagcggata ttattcattg caatgcctgc attatcaata gtaagggacg tctgcttggc
    24721 tattttatgc gttataaaac aaatacagat cgcgtagagc aattcttcca aactaagatt
    24781 ttcccagatg actacgttca aggggctaat atgatttacg aaacagaagc aaacttgcct
    24841 gttgagcatg atatgagtat tttccctgtt gagagtagag atgattttcc agatggcttg
    24901 acgactattg caccgattca tgtatcgggg attcgccttg gttctttgat tatttggcgt
    24961 aatgataaaa aattcgaaga tgaggacttg gttcttgttg agattgccag taccgttgtt
    25021 gggattcagc ttcttaactt ccaacgtgaa gaagatgaga aaaatattcg tcgtcgtact
    25081 gctgtcacca tggcggtcaa taccctttct tactccgaac tccgtgctgt ttcagcaatt
    25141 ttaggggaat taaatggaaa tgaagggaag ttgactgcgt cagtgattgc agatcgtatc
    25201 ggaatcactc gctctgtgat tgtcaatgct cttcgtaaac ttgagtctgc ggggattatt
    25261 gaaagtcgct cacttggaat gaagggaacc tatcttaagg tcttgatttc agatattttt
    25321 gaagaagtga agaaaagaga ttactaatga caaaggcttt aatttcgatt gattatacag
    25381 aagattttgt tgctgatagt gggaagctga cagcaggtgc tccagctcag gcgatttcgg
    25441 atgccatcag caaggtaact cgattagctt ttgaacgagg agattatatc ttctttacta
    25501 ttgatgctca tgaagaaaac gattgtttcc atccggaaag taagttattt cctcctcata
    25561 atctgattgg gacgagtgga cggaatttat atggagattt ggggatcttt tatcaagagc
    25621 atggttcaga cagtcgtgtc ttttggatgg ataaacgcca ttactcagct ttttcaggga
    25681 ctgacctaga tattcgtttg agagagcgta gagtgtctac cgttatctta acaggtgtct
    25741 tgacggatat ctgtgtccta catacagcta tagatgccta taatctagga tatgacatcg
    25801 agattgttaa accagctgtt gcttccatct ggcctgaaaa tcatcaattt gccctaggtc
    25861 atttcaaaaa tacactcgga gctaagttag tagatgaaaa tctaaatgaa ctttctgagt
    25921 aacctggttg gtgaaaatga aaaaatttga aaaaaagtgt tgacaagcat cccaaaagtt
    25981 gatatactag taaagtaatc gatgcgggga tggcggaatt ggcagacgcg caggactaag
    26041 gatcctgtga ccgctttagg tcgtgagggt tcaagtccct ctctccgcat aggaaaagag
    26101 ttagctaaag ctagctcttt tgtatttctc taaaaaatta gaatccagtc aaatgtagtg
    26161 gagaatcagc gaaaatatgg gaaaactgat ccttgtttct gcttttgtca tagaagaata
    26221 ataaagatta tcttcttcaa attctccgat attctctaaa gttttgtgca agttgcacag
    26281 aacttttttt atttttttgg tcatcttgcc atagaaatat aaagcgtttt catctataat
    26341 ataattatca aaagacaaaa ggagttcacc tcatggtaga attgaatctt aaaaatattt
    26401 acaaaaaata tccaaacagc gaacactatt cagttgaaga tttcaacttg aacatcaaag
    26461 ataaagaatt tatcgttttc gtaggacctt caggatgtgg taaatcaact acactccgta
    26521 tgattgctgg tcttgaagac atcacagaag gtactgcatc tatcgatggc gtggttgtca
    26581 acgacgtagc tccaaaagac cgtgatatcg ccatggtatt ccaaaactac gctctttacc
    26641 cacacatgac tgtttatgac aacatggctt tcggtttgaa attgcgtaaa tacagcaaag
    26701 aagacattaa caaacgtgtt caagaagcag ctgaaatact tggattgaaa gaattcttgg
    26761 aacgtaaacc ggctgacctt tcaggtggtc aacgtcaacg tgttgccatg gggcgtgcga
    26821 ttgtccgtga tgcgaaagta ttcttgatgg acgaaccttt gtcaaacttg gatgccaaac
    26881 ttcgtgtatc aatgcgtgct gaaatcgcta aaattcaccg tcgtatcgga gctacaacta
    26941 tctatgtaac tcacgaccaa acagaagcga tgacacttgc agaccgtatc gttattatgt
    27001 cagctactaa gaaccctgct ggtacaggta ctatcggacg tgtagaacaa atcggtactc
    27061 ctcaagaagt ttacaaaaat ccagttaaca aattcgttgc aggattcatc ggaagcccag
    27121 ctatgaactt catcaacgtg aaattggttg gtagcgaaat tgtttctgac ggtttccgtt
    27181 tgaaagtgcc agaaggagca ttgaaagttc ttcgtgaaaa aggctacgaa ggaaaagaat
    27241 tgatctttgg tatccgtcca gaagacgtga atgcagaacc tgctttcctt gaaacattcc
    27301 cagactgtgt tgtaaaagcg actatctctg tatcagaact gcttggttca gaatctcacc
    27361 tttactgcca agttggtaaa gacgagtttg ttgcaaaagt tgatgctcgt gactacttgc
    27421 aaacaggtgc aacagttgag cttggatttg acttgaacaa agcacacttc ttcgatgtag
    27481 aaactgaaaa aacaatctac taagaataaa taaaattcaa agcactacaa gaaaagatat
    27541 ctctttatca attgtagtgg agagatatca gttaatctag ggagagaaac aaaatgcttc
    27601 tctccttttt gctagggaag tcatattata catctatatt gtgatgctct ttatactctt
    27661 cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta cgtatgatta ctgacttcgt
    27721 cagttttatc tgcaacctca aagctgtact ttgagtagct tacggctagt ttcctagttt
    27781 gctctttgat ttccattgag tattatttgt gggtaccatc tacaagtgaa gctatatgcg
    27841 taaactacgt gagcaattga attcgaacta gagaggtaat aataaattta tgctatagtt
    27901 atggtgactt gtatgctttt gattctagtt tatcaaataa tagattagaa ttgtcagata
    27961 atatcatttt gtgttataat gaagaaaaaa cagaggtgtt caaatgtcag aagcaggtca
    28021 taagttttta gcaaaattgg ggaaaaaacg cttacgtcca ggtggaaagc gtgccacaga
    28081 ttggttaatt gcagaaggag gattttcaaa agaaaagaga atactagagg ttgcgtgtaa
    28141 taggggaact acagcaattg agttggcaca gcgttttggt tgcaagataa ctgctgttga
    28201 tatggatgct caagctttag aagtggctaa aaaatctgct ggaacggcag gtgttgctca
    28261 tttaatcagt tttgaaagag caaatgcaat gaaacttcct tatcaagatg ctagttttga
    28321 tattgttata aatgaagcta tgctgactat gcaagccgat caagctaaga aaaaatgtgt
    28381 aatggaatat ctaagggtat taaaacctgg aggtcttctc ttgacacatg atgtgcttct
    28441 taaggaagct aaagagtcta tcagacagga attatcacaa gcaattcatg taaatgtagg
    28501 tcctttaact caagatggtt gggaacaggt gatgatagaa tcaggttatt gtgatgtgaa
    28561 agcattgact ggtgaaatga cattaatgaa attatcgggt atgatttatg acgaaggttt
    28621 gctaggaact ttgaaaattt gtgtaaatgc ttgtaaaaag gagaatagaa agcagttttt
    28681 aactatgtat aaaatgtttg ctaagaataa acagaaattg ggctttattg cgatggctag
    28741 ttataaatcg tcaaaacgtt agataattat tgaagttaac ttttcctttt ttctttctta
    28801 aaaaatatgc tataatagag agtaaaaaac tttgaaagaa agaaaaaaga tgaatttaaa
    28861 agattacatt gcaacaattg aaaattatcc aaaggaaggc attaccttcc gtgatattag
    28921 tcctttgatg gctgatggaa atgcttatag ctacgctgtt cgtgaaatcg ttcagtatgc
    28981 tactgacaag aaagtcgaca tgatcgtggg acctgaagct cgtggattta tcgtgggttg
    29041 tccagttgcc tttgagttgg gaattggttt tgcgcctgtt cgtaagccag gtaaattgcc
    29101 acgcgaagtt atttctgctg actatgaaaa agagtacggt gtcgatacct tgactatgca
    29161 cgcggatgcc attaagccag gtcaacgtgt tcttattgta gatgaccttt tggcgacagg
    29221 tggaactgtt aaggcaacta tcgagatgat tgaaaaactt ggtggtgtta tggcaggttg
    29281 tgccttcctt gttgaattgg atgaattgaa cggccgtgaa aaaatcggtg actacgacta
    29341 caaagttctt atgcattatt aatgaaaaca gtccctaggg ctgttttctc tacactagga
    29401 tataaaaata gactataact agttagagaa aaactataat tgaaaactat atcttcttgc
    29461 agtataataa aaggactaag tgtttgagat ttgtcttcaa acacatgcaa ttattcctga
    29521 gagagtacag ttaggagagg gttatgccga ttcgaattga taaaaaattg ccagctgttg
    29581 agattttacg gacagagaat atctttgtca tggatgatca acgtgctgcc caccaagata
    29641 tccgtccttt gaagatttta attttaaatc tcatgccaca gaaaatggtc acagagaccc
    29701 agttgttgcg ccacttggct aatacacccc tacaactgga tattgatttt ctctatatgg
    29761 agagccaccg ttctaaaaca actcgttcag agcacatgga gaccttctat aaaacttttc
    29821 ctgaagtcaa ggatgagtat tttgatggga tgatcatcac gggtgctcca gttgagcatt
    29881 taccatttga ggaagtggac tattgggagg aatttagaca gatgcttgag tggtctaaga
    29941 ctcatgtcta ttcgaccctt catatctgtt ggggagctca ggctgggctt tatctgcgct
    30001 atggtgtaga aaaataccag atggacagta agctatcagg tatttatcct caggacaccc
    30061 taaaagaggg tcaccttcta tttagaggct ttgatgatag ctatgtatcc cctcattcac
    30121 ggcacacgga gatttctaag gaagaggtct taaacaagac taatctcgag attttatcag
    30181 aaggacctca ggttggggtt tctattttgg ccagtcgtga tttacgagaa atttatagtt
    30241 ttggtcattt ggagtatgac cgtgatacct tggcaaaaga gtattttcga gatcgtgatg
    30301 caggtttcga cccacatatt ccagaaaatt actttaaaga tgatgatgtt aatcaggtac
    30361 cttgtctttg ttggtcttca tctgcagccc tctttttcag taattgggta aaccatgcgg
    30421 tctatcagga gacgcctttt gactggagaa agatagaaga tgatgcatct gcatatgggt
    30481 atttataaga ggaattatga catatttaga cgcttttaaa tcaggtacct tggttttacc
    30541 gagtgccctg ctcttgcatt ttaaggaact ctttccttct agcgacgatt ttctggtttg
    30601 gcaatttttc tatttgcaaa atacgacagg cttagaagaa atgtcgccaa gccagattgc
    30661 tgaaaggatt ggcaaggaaa tttcggatgt caaccagtcc atttctaatc tgacggaaag
    30721 gggactgctc cagtatcgta ctatcgaatt aaatggcgaa attgaattgc tctttgatgc
    30781 tagtttggcc ttggaacgtt tggatgacct gtttggagca gttcattcaa gttcagacca
    30841 gctaacacca caaaaccagc tcaaggattt ggtggaaacc ttccagcagg agttgggacg
    30901 attgttgacg ccttttgaga ttgaggattt gaccaagaca ctaaaggaag atggaaccag
    30961 tgctgacttg attaaggagg ctcttcgtga agctgttttg aatggaaaac caaactggaa
    31021 gtacattcag gcgattttga gaaactggcg ccatgaagga atcaagagtg tggctcaaat
    31081 tgaggccaag agagcagaaa gagaagcaag caatcctcag ttgacacagg tatctgcaga
    31141 tttcagaaat gccatggatc tctggaagga ttaatccatg caagtaggct tgaaatccga
    31201 gtaagatttg caagctgtgt ataattgtga tagaataaat agaaaataaa ttgaaaaaag
    31261 aggtatgtga aatgtcacgt aaaccattta tcgctggtaa ctggaaaatg aacaaaaatc
    31321 cagaagaagc taaagcattc gttgaagcag ttgcatcaaa acttccttca tcagatcttg
    31381 ttgaagcagg tatcgctgct ccagctcttg atttgacaac tgttcttgct gttgcaaaag
    31441 gctcaaacct taaagttgct gctcaaaact gctactttga aaatgcaggt gctttcactg
    31501 gtgaaactag cccacaagtt ttgaaagaaa tcggtactga ctacgttgtt atcggtcact
    31561 cagaacgccg tgactacttc catgaaactg acgaagatat caacaaaaaa gcaaaagcaa
    31621 tctttgcgaa cggtatgctt ccaatcatct gttgtggtga atcacttgaa acttacgaag
    31681 ctggtaaagc tgctgaattc gtaggtgctc aagtatctgc tgcattggct ggattgactg
    31741 ctgaacaagt tgctgcctca gttatcgctt atgagccaat ctgggctatc ggtactggta
    31801 aatcagcttc acaagacgat gcacaaaaaa tgtgtaaagt tgttcgtgac gttgtagctg
    31861 ctgactttgg tcaagaagtc gcagacaaag ttcgtgttca atacggtggt tctgttaaac
    31921 ctgaaaatgt tgcttcatac atggcttgcc cagacgttga cggtgccctt gtaggtggtg
    31981 cgtcacttga agctgaaagc ttcttagctt tgcttgactt tgtaaaataa tcagtaagta
    32041 gcaaaagcta ggtggaacag cattcagatg tctgttacat tttttatagg agagcaaaga
    32101 ttgaaaacaa aaattggatt agcaagtatc tgtttactag gcttggcaac tagtcatgtc
    32161 gctgcaaatg aaactgaagt agcaaaaact tcgcaggata caacgacagc ttcaagtagt
    32221 tcagagcaaa atcagtcttc taataaaacg caaacgagcg cagaagtaca gactaatgct
    32281 gctgcccact gggatgggga ttattatgta aaggatgatg gttctaaagc tcaaagtgaa
    32341 tggatttttg acaactacta taaggcttgg ttttatatta attcagatgg tcgttactcg
    32401 cagaatgaat ggcatggaaa ttactacctg aaatcaggtg gatatatggc ccaaaacgag
    32461 tggatctatg acagtaatta caagagttgg ttttatctca agtcagatgg ggcttatgct
    32521 catcaagaat ggcaattgat tggaaataag tggtactact tcaagaagtg gggttacatg
    32581 gctaaaagcc aatggcaagg aagttatttc ttgaatggtc aaggagctat gatgcaaaat
    32641 gaatggctct atgatccagc ctattctgct tatttttatc taaaatccga tggaacttat
    32701 gctaaccaag agtggcaaaa agtgggcggc aaatggtact atttcaagaa gtggggctat
    32761 atggctcgga atgagtggca aggcaactac tatttgactg gaagtggtgc tatggcgact
    32821 gacgaagtga ttatggatgg tgctcgctat atctttgcgg cctctggtga gctcaaagaa
    32881 aagaaagatt tgaatgtcgg ctgggttcac agagatggta aacgctattt ctttaataat
    32941 agagaagaac aagtgggaac cgaacatgct aagaaaatca ttgatattag tgagcacaat
    33001 ggtcgtatca atgattggaa aaaggttatt gatgagaacg aggtggatgg tgtcattgtt
    33061 cgtctaggtt atagcggtaa agaagacaag gaattggcgc ataacattaa ggagttaaac
    33121 cgtctgggaa ttccttatgg tgtctatctc tatacctatg ctgaaaatga gaccgatgct
    33181 gagaatgacg ctaaacagac cattgaactt ataaagaaat acaatatgaa cctgtcttac
    33241 cctatctatt atgatgttga gaactgggaa tatgtaaata agagcaagag agctccaagt
    33301 gatacagaca cttgggttaa aatcatcaac aagtacatgg acacgatgaa gcaggcgggt
    33361 tatcaaaatg tgtatgtcta tagctatcgt agtttattac agacgcgttt aaaacaccca
    33421 gatattttaa aacatgtaaa ctgggtagcg gcttatacga atgctttaga atgggaaaac
    33481 ccttattatt caggagaaaa aggttggcaa tatacctctt ctgaatacat gaaaggaatc
    33541 caagggcgcg tagatgtcag cgtttggtat taagcgatga tttgaaagag ggatgtgata
    33601 gtagcaccct ctttttcttt gttttatgat agttcatcct cgagtaaatt caagatcttg
    33661 ctcggaaatg aagcttatat agtagattga atatagacaa ataccttgtg attggtaaaa
    33721 cattttagaa attcatttac ctttcctaat cgacttggtt tcatcttatt tcaatctatt
    33781 atagtattgg ggaatttctt caaaccacat cagcttggtc agttctacct gcgacctcaa
    33841 aacttgtgct ttggtcaagc tgggtttagt ttcctagttt gctgatggat ttccattgac
    33901 tataagcatc caaccctctt tttgtcttct aaagaattct taaattatca gtctattgca
    33961 acttttctca tgtgggtcat ttggcttgca attgtctttc cttagtagta tactgaggta
    34021 gtaatcatta agaagtggtt acataaaaat aatgaatgag gtaaaataaa atggtagaat
    34081 tgaaaaaaga agcagcaaaa gatgtcgcaa cattaacaaa agcagcgcca gtagcattgg
    34141 caaaaacaaa ggaagttttg aatcaagcag ttgctgattt gtgtgtagct cacgttgctt
    34201 tgcaccaagt gcactggtat atgcatggtc gtggtttcct tgtatggcat ccaaaaatgg
    34261 atgagtacat ggaagctctt gatggtcatt tagatgaaat cagcgaacgc ttgatcacac
    34321 ttggtggaag cccattctct actttgacag agttccttca aaacagtgaa atcgaagaag
    34381 aagctggtga ataccgtaat gttgaagaaa gcttggaacg tgttctagct atttatcgct
    34441 acctcatcac tcttttccaa aaagcgttgg atgtgactga cgaagaaggc gatgatgtta
    34501 caaacgatat ttttgttggt gctaaggctg aacttgagaa aacagtttgg atgcttgccg
    34561 cagaacttgg acaagcacct ggtttgtaag aaacaagaca acatagataa atctgtagga
    34621 aatttcttac agattttttc tattttgtaa gctattccaa accagtcttt ttttgagttt
    34681 tttgataaaa tagtactatc agtgaaaagg atggaagcat gactaagaaa atcgtagcta
    34741 tttgggccca ggatgaagag ggtgtgattg gtaaggacaa tcgtctgcct tggcatttgc
    34801 cagcagaatt gcagcacttt aaagaaacaa ctctgaatca tgctatcttg atggggcggg
    34861 taacctttga tggaatgggg cgtcgcttgc ttccaaaacg ggagaccctg attttgacgc
    34921 gtaatccgga agaaaagata gatggggttg ctacttttca ggacgtccag tctgttcttg
    34981 actggtatca ggctcaagaa aagaatctct acattatcgg tgggaagcaa atttttcagg
    35041 cttttgaagc ttaccttgat gaagtgattg tcactcacat tcatgctcgg gtggaaggag
    35101 atacctattt ccctgaagag cttgacttgt ctctttttga gacagtttca agcaaatttt
    35161 acgccaaaga tgagaagaat ccttatgatt ttaccatcca ataccgcaag agaaaggaag
    35221 tttaatggaa cggagtttat ttggtctatt cacggctttt ctgtgtttca tctgctttct
    35281 ggcaggagca caagcttttc gtaaaaagcg ttacgggctt tctatcctcc tttggttaaa
    35341 tgcctttacc aatctggtca atagtattca tgctttttac atgaccttat tttagataga
    35401 atgacagtat agaatggaag gaaatcatgt ctacaaatag aaaaaatgat atgatggttt
    35461 attgctcatt ttgtggcaaa aaccaagaag aagtacaaaa aataattgct ggcaacaatg
    35521 cttttatttg taatgaatgc gtggagttag ctcaggaaat cattcgagaa gaattggttg
    35581 aggaagtctt ggcagacttg tctgaggtgc caaaaccaat tgaactcctc catatcttga
    35641 accactatgt aattggtcaa gatcgtgcca agcgtgcctt ggcagtggcg gtttataacc
    35701 actacaaacg catcaatttc cacgatacac gcgaagagtc agaagatgtg gatttgcaga
    35761 agtcaaacat tttgatgatt ggcccaactg gttcagggaa aactttcctt gcccagacct
    35821 tggctaagag cttgaatgta ccttttgcta ttgcggatgc gacagctctg acggaggctg
    35881 gttatgtggg tgaggatgtg gaaaatatac tcctcaaact cttgcaggtt gctgacttta
    35941 acatcgaacg tgcagagcgt ggcattatct atgtggatga aattgacaag attgccaaga
    36001 agagtgagaa tgtgtctatc acacgtgatg tttctggtga aggggtgcaa caagcccttc
    36061 tcaagattat cgagggaact gttgctagcg taccgcctca aggtggacgc aaacatccac
    36121 aacaagagat gattcaagtg gatacaaaaa atatcctctt catcgtgggt ggtgcttttg
    36181 atggtattga agaaattgtc aaacaacgtc tgggtgaaaa agtcatcgga tttggtcaaa
    36241 acaataaggc gattgacgaa aacagctcat acatgcaaga aatcatcgct gaagacattc
    36301 aaaaatttgg tattatccct gagttgattg gacgcttgcc tgtttttgcg gctcttgagc
    36361 aattgaccgt tgatgacttg gttcgcatct tgaaagagcc aagaaatgcc ttggtgaaac
    36421 aataccaaac cttgctttct tatgatgatg ttgagttgga atttgacgac gaagcccttc
    36481 aagagattgc taataaagca atcgaacgga agacaggggc gcgtggactt cgctccatca
    36541 tcgaagaaac catgctagat gttatgtttg aggtgccgag tcaggaaaat gtgaaattgg
    36601 ttcgcatcac taaagaaact gtcgatggaa cggataaacc gatcctagaa acagcctaga
    36661 ggtgactatg gaacttaata cacacaatgc tgaaatcttg ctcagtgcag ctaataagtc
    36721 ccactatccg caggatgaac tgccagagat tgccctagca gggcgttcaa atgttggtaa
    36781 atccagcttt atcaacacta tgttgaaccg taagaatctc gcccgtacat caggaaaacc
    36841 tggtaaaacc cagctcctga acttttttaa cattgatgac aagatgcgct ttgtggatgt
    36901 gcctggttat ggctatgctc gtgtttctaa aaaggaacgt gaaaagtggg ggtgcatgat
    36961 tgaggagtac ttaacgactc gggaaaatct ccgtgcggtt gtcagtctag ttgaccttcg
    37021 tcatgccccg tcagcagatg atgtgcagat gtacgaattt ctcaagtatt atgagattcc
    37081 agtcatcatt gtggcgacca aggcggacaa gattcctcgt ggtaaatgga acaagcatga
    37141 atcagcaatc aaaaagaaat taaactttga cccgagtgac gatttcatcc tcttttcatc
    37201 tgtcagtaag gcagggatgg atgaggcttg ggatgcaatc ttagaaaaat tgtgaggaaa
    37261 agaaaatggc aaaaacaatt catacagata aggccccaaa ggctatcgga ccctatgttc
    37321 aaggaaaaat cgttggcaac cttttgtttg ctagcggtca agttccccta tcccctgaaa
    37381 ctggggaaat tgtaggagag aatatccaag aacagacaga gcaagtcttg aaaaacatcg
    37441 gtgctatttt ggcagaagca ggaacagact ttgaccatgt tgtcaaaaca acttgcttct
    37501 tgagcgatat gaacgacttt gttcctttta atgaggttta ccaaacggcc ttcaaagagg
    37561 aattcccagc tcgttcagct gtggaggtag ctcgtcttcc tcgtgatgta aaagtcgaaa
    37621 ttgaagtcat cgcagagatt ggataagcta gttgaagttt ggtgttgcca aacttctttt
    37681 gatataagga gaaaaagatg acaaagaaac aacttcactt ggtgattgtg acagggatga
    37741 gtggcgcagg gaaaactgta gccattcagt ccttcgagga tctaggttat ttcaccattg
    37801 ataatatgcc gccagctctc ttgcctaagt ttttgcagct ggttgaaatt aaggaagaca
    37861 atcctaagtt ggccttggta gtggatatgc gtagccgttc tttcttttca gagattcaag
    37921 ctgttttgga tgagttggaa aatcaagatg gtttggattt caaaatcctc tttttggatg
    37981 cggctgataa ggaattggtc gctcgttaca aggaaaccag acggagtcac ccactagcag
    38041 cagacggtcg tattttagat ggaatcaagt tggaacgtga actcttggca cctttgaaaa
    38101 atatgagcca aaatgtggtg gatacgactg aactcactcc acgtgagctg cgtaaaaccc
    38161 ttgcagagca gttttcagac caagaacaag cccagtcttt ccgtatcgaa gtcatgtctt
    38221 tcggatttaa atatggaatc ccgattgatg cggacttggt ctttgatgtc cgtttcttgc
    38281 caaatcccta ttatctacca gaactgagaa accaaacggg tgtggatgaa cctgtttatg
    38341 attatgtcat gaaccatcct gagtcagaag acttttatca acatttattg gccttgattg
    38401 agccgattct gccaagttac caaaaggaag gtaagtccgt tttgaccatt gctatgggat
    38461 gtacgggtgg acaacaccgt agtgtggcat ttgctaaacg cttggtgcag gacttatcca
    38521 agaattggtc tgttaatgaa gggcatcgcg acaaagaccg cagaaaggaa acggtaaacc
    38581 gttcatgaga aaaccaaaga taacggtgat tggtggaggg actggaattc ccgtcattct
    38641 aaaaagtctg cgggaaaaag atgtggaaat cgcagctatc gtgacggtgg cagatgatgg
    38701 tggttcttca ggtgaactcc gaaaaaatat gcaacagttg acaccgccag gtgatcttcg
    38761 taatgtcctt gtggccatgt cggatatgcc taagttttat gagaaggtct ttcagtatcg
    38821 gttctctgag gatgccggag cctttgctgg ccatccattg ggaaatctca tcattgctgg
    38881 cttgtcagaa atgcagggtt caacctataa tgccatgcag ttattgagca aatttttcca
    38941 tacaacaggg aaaatttatc cttccagtga ccatcctttg acccttcatg cagtctttca
    39001 ggatgggaca gaagtggctg gagagagtca tattgtagac catcgaggca taattgacaa
    39061 tgtctatgtg accaatgccc taaacgatga tacgcctctg gccagccgtc gagtagtgca
    39121 gaccatcctt gaaagtgaca tgattgtcct agggccaggt tccctcttta cctctatttt
    39181 gcccaatatc gtgattaagg aaattgggcg ggctcttttg gaaactaagg cagaaattgc
    39241 ctatgtctgc aatatcatga cccaacgtgg ggagacggaa cactttacag atagcgacca
    39301 cgtggaagtc ttgcatcgtc accttggtcg cccttttatc gacactgtct tggtgaatat
    39361 tgaaaaagtg cctcaggaat acatgaattc caaccgtttt gatgaatact tagtgcaagt
    39421 ggaacacgat tttgtaggtc tttgtaagca agtttcgcgc gtgatttcat ctaacttcct
    39481 tcgtctggaa aatggcggtg ccttccacga tggagatttg attgtggacg agttgatgcg
    39541 cattatacag gtgaaaaaat gagtttcaca gtagcagtaa aagaagaaat cctgggccaa
    39601 caccatctga gccggcatga attatctgcc attatcaaaa tgtctggtag catcggtctc
    39661 tcgacttcgg gcttgacttt gtcagttgtg acagaaaatg ccaaactggc ccgtcacctc
    39721 tatgagtcct ttctccattt ctatgaaatc aaatctgaaa ttcgtcacca ccaacggagc
    39781 aatcttcgca agaatcgcgt ctataccgtt tttacagatg aaaaggtgca ggatttgtta
    39841 agtgatttgc acttggcaga ctctttcttt ggcctggaaa caggtattga tgaggcgatt
    39901 ttatcggacg aggaagcagg tcgtgcctat ctctgtggcg ctttcttggc aaatgggagc
    39961 attcgtgacc ctgagtcagg caagtaccag ttggaaatca gttctgttta tctggaccac
    40021 gcgcaaggaa ttgcctccct tctccaacag tttttactgg atgccaaggt gcttgagcgc
    40081 aagaaggggg ctgtgaccta tctccagcga gcagaagaca ttatggactt cttgatagta
    40141 ataggagcca tgcaggcacg tgatgatttt gagcgggtta agattttgcg agaaacccgt
    40201 aacgacctca atcgggctaa taatgccgag acagctaata tcgctcggac agtttctgcc
    40261 agcatgaaga ccattaacaa tatcagtaaa atcaaagata tcatgggctt agaaaatttg
    40321 ccagtagatt tgcaggaagt ggcgcaactg aggattcagc acccagacta ctctatccag
    40381 cagttagcag atagtctcag cacccctctg accaaaagtg gtgtcaatca cagactcaga
    40441 aaaatcaata agatagcgga tgaattataa aaccacgaat cctatgtgac tcgtggttct
    40501 tttttataaa ctggtagagt gttttggttg tactttttgt tcagggtcaa tgtagttgat
    40561 agcgttgttg acagcagttg gagcttctcc gaggcctgtc gcaatcagat caatttttcc
    40621 gtcatagtag cagcagtcac cgatagcata gatacctgct tggctggatt cctgtttgct
    40681 gttgacgata atcttgtgac ggttgaggtc gagcccccag tttttaaggt taccgacaga
    40741 agatttgaaa ccatagttga caaagaggtg gtctaggtca atggtttcag tttcatcaga
    40801 tttgactttt gtgatttcaa gtttatcaag tgtttttcca tttccaagga gttggctagg
    40861 ggcgaatggt gtcttgatgg ttacagatga ttcttgcaag gtttgaacac tgtgttccaa
    40921 ggcacggaaa ttatctctgc ggtgaacaag ggtagttggt gcgatttttt caaaagccaa
    40981 agcccaatcc acagccgagt ctcccccacc aagaatcgtc actttcttac cagcgtattg
    41041 ctgaatgtta gaaacgtggt agtggatatt ttcatagtcc tcaacccctt caagttccag
    41101 cggacgtggt ttgaaggcac cgccacccat agcgatgata actgttttag tcaggtgact
    41161 tcctttggaa gttgtgatgg caaattcttc ttcttgtttg tcaatctcaa gaaccgtttc
    41221 attgagatga ataggggtat caaatccatt tagctgttca atcaagcggt tagtcaactc
    41281 ttctccagtc aggtttggga agcctggtac gtctaggatt tccttttcag ggtagagaat
    41341 agcaggttgt ccacctagct ggggaagaga gtcgatgatt tgaaccttgg cttggcgtag
    41401 gtgggcataa aaggctgcaa aaagcccgac aggaccacca cccacaatgg taatatcata
    41461 gagttgagac atagtttctc ctttgttttt tctagtcagt ttattttatc atatttttgt
    41521 tcaatgtaaa atggactagg atagggaaaa aaatgccaga aaatggtata ataggacgaa
    41581 agtgttttga tagggaggat atcagggatg aattttcaac aattatccaa tctgcaatat
    41641 tggaccagtt tgtttgcaag tccatggacg atagctatca atctgattga tattttgatt
    41701 gttgcttata ttttatacca ttttacaaaa gctattgcag gaaccaagat tatgattttg
    41761 gtacgtggag ttttggtgtt tattttagct caaatccttg caaatatgat tggtttgact
    41821 acgatttctt ggttaatcaa tcaaattatt acttatgggg ttattgcggc ggttgttatc
    41881 ttctctccag agattcggac tggtttggaa cgtttgggaa gagcgacaga tttcttttcc
    41941 aatgccccta ttagtgctga ggaacagatg attcgtgcct ttgttaagtc tgttgaatac
    42001 atgagtcctc gtaaaatcgg ggccttggtt gctattcagc gtgtacgtac cttgcaggag
    42061 tatatgtcga caggaattcc cttggatgct aagatttctg cagaacttct cattaacatt
    42121 tttattccca acactcccct acatgacggt gcggtgatta tcaaagaaga acgtatcgct
    42181 gtgacgtctg cctatctgcc cttgacaaaa aacacaggga tttccaagga atttgggacc
    42241 agacaccggg cggctatcgg tttatcagaa gtctcagatg ccttgacttt tgtcgtatca
    42301 gaggaaacgg gaggaatttc gataacctat aatggaaggt ttaagcacaa cctaacactt
    42361 gatgaatttg aaacagaatt acgtgaaatc ttacttccaa aagaggaagt gggtcttagt
    42421 tttaaagaac gattgctagg aggatggaaa catgaaaaaa aatagtttat atatcatatc
    42481 ctcactcttt tttgcttgtg tcttatttgt ctatgctacg gcgacgaatt ttcaaaacag
    42541 taccagtgct aggcaggtaa aaacggaaac ctatactaat acagtaacca atgtccctat
    42601 tgacatacgc tataatagtg ataagtattt tattagcggt tttgcttcag aagtatcagt
    42661 ggtcttgact ggtgcaaatc gcctatcgct agctagtgaa atgcaagaaa gtacacgtaa
    42721 attcaaggtt actgctgacc taacagatgc cggtgttgga acgattgaag ttcctttgag
    42781 cattgaagat ttacccaatg ggctgaccgc tgtggcgact ccgcaaaaaa ttacagtcaa
    42841 gattggtaag aaggctcaga aggataaggt aaagattgta ccagagattg accctagtca
    42901 aattgatagt cgggtacaaa ttgaaaatgt catggtgtca gataaagaag tgtcgattac
    42961 gagtgaccaa gagacattgg atagaattga taagattatc gctgtcttgc caactagcga
    43021 acgcataaca ggtaattaca gtggttcagt acctttgcag gcaatcgatc gcaatggtgt
    43081 tgtcttaccg gcagttatca ctccgtttga tacaataatg aaggtgacta caaaaccagt
    43141 agcaccaagt tcaagcacat caaattcaag tacaagcagt tcatcggaga catcttcgtc
    43201 aacgaaagca actagttcaa aaacgaatta aaaatagaaa aggattttat aacaatgggt
    43261 aaatattttg ggactgatgg agtccgtgga gaagctaacc tagagctaac accagaatta
    43321 gcctttaaac taggacgttt tggaggctat gttcttagtc aacatgaaac ggaagcgccg
    43381 aaagtctttg taggacgtga cacacgtatt tcaggggaaa tgctggaatc ggccttggtg
    43441 gcaggtctcc tttcagtagg gattcacgta tacaaacttg gtgtccttgc aacaccagca
    43501 gtagcttact tggttgaaac tgaaggagca agtgccggtg tcatgatttc tgctagccac
    43561 aacccagccc ttgataacgg aatcaagttc tttggcggtg atggcttcaa actagatgat
    43621 gaaaaagaag cagaaattga agccttgcta gatgctgagg aagacactct tcctcgtcca
    43681 agtgcagaag gcttaggaat tttggtagat tatccagaag gcttgcgtaa gtatgaagga
    43741 taccttgttt caactggaac tcctcttgat ggaatgaagg ttgccttgga tacagctaat
    43801 ggagcagctt ctaccagtgc ccgtcaaatc tttgcagacc ttggtgccca attgacggtt
    43861 atcggggaaa caccagacgg tcttaacatc aaccttaatg ttggttcaac acatccagaa
    43921 gcccttcaag aagtggtcaa agaaagtggg tcagctattg gtttggcctt tgatggagac
    43981 agtgaccgct tgattgctgt tgatgagaat ggtgacatcg tcgatggtga caagattatg
    44041 tacatcatcg gaaaatacct ttctgaaaaa ggacaattgg ctcaaaatac aattgtgaca
    44101 actgttatgt ctaaccttgg tttccacaag gccttgaatc gcgaaggtat taacaaggca
    44161 gttactgcag ttggtgaccg ctacgttgtt gaagaaatga gaaaatcagg ttacaacctt
    44221 ggtggtgaac agtctggtca cgttatcttg atggattaca ataccacagg tgatggtcaa
    44281 ttatcagcag ttcaattgac taaaatcatg aaggaaactg gtaagagctt atcagagttg
    44341 gcggcagaag taacgattta tccacaaaaa ttagttaata tccgagtgga aaacgtcatg
    44401 aaggaaaagg ccatggaagt gccagctatc aaggccatca tcgagaagat ggaagaagaa
    44461 atggcgggga acggccgtat ccttgttcgt ccaagtggaa cagaacccct cttgcgtgtt
    44521 atggcagaag cgcctacaac agaagaagta gactactatg ttgataccat cacagatgta
    44581 gttcgtgctg aaattgggat tgactaaatc ctagaaaact agatgaaaat aaaaaattat
    44641 ggtacaatag cttataatat tgtagccgat ggagaaagac atgaatctta aacgtgaaca
    44701 agaatttgtt agtcagtatc attttgatgc gcgtaatttt gaatgggaaa atgaaaatgg
    44761 agctcctgaa accaaggtgg atgtgaattt ccaattgctc caacatgacc aagaaaatca
    44821 agtgacttcc ttgattgtca tcttgagctt tatgattgta tttgataagt ttgtcattag
    44881 tggaacaatc tcacaagtaa accatatcga tggccgtatt gtcaatgaac caaatgaatt
    44941 gaaccaagaa gaggtggaaa ccttggctcg tccatgtttg aacatgctca atcgtttgac
    45001 ttacgaagtg actgaaattg cattagactt accaggaatt aatttggagt tctaatatga
    45061 aattagctgt tttcacagat tcctctgctt atctcagtgc agagatcttg caaagagaag
    45121 acttgtttgt cttggatatt cctgtcaata ttgatggtga ggaatatgtc gaaggcatca
    45181 atctgtctgc tgaggaattt taccaaaaaa tggctcaggc ttctgaattg cctaagacca
    45241 gtcaaccaag tattgccaag ttagatgaga tcttaacttc gctcaaagaa caaggctata
    45301 cacatgcctt ggggcttttc ctatcttctg gaatttcagg tttttaccaa aatatccagt
    45361 atatggtcga tgactatgag ggcttaacca ttgctttccc agacactttg attacaagtg
    45421 ctcccctagg catcatggtt gaaagtgtct ttaattggcg tgatcagggc gatgattttg
    45481 ccagcattca ggataagcta gccattcaaa tcagccgtac gtcagccttt atcatggtag
    45541 atgacttgga tcatttggta aaaggtggac gcctttcaaa tggggctgcc attttgggca
    45601 atttgcttag cattaagcca atcctttatt ttaacgatca aggtgtgatt gaagtttacg
    45661 aaaaagttcg tactgaaaag aaggccacca agcgcttaat tgaaattatc aaggaaacaa
    45721 cggcttcagg tcaataccgg gtcattgtca ttcatgggaa tgctcctgaa aaggctgaag
    45781 aattgcgtca gcacttgctt gattttggct tgggttcgga tgtttcactt gctacatttg
    45841 gtagtgtcat tggaacgcac ctaggagcag gaagtattgc tctgggttat attccagtga
    45901 tttagttggc gcttctaggc tagttaagat gccttgtatc ttagaaattg aacacggact
    45961 acctgcctct tgaaaaaaga tgcctgtctt gcctttcgtg gaaagtcagt gccattccct
    46021 atttttcaga ggcagctaag gtcctttgta tcttgataat tgaacacgcc tggaaccctg
    46081 tgtgaaaaag atagttcttc caagaagtag acacttcttg attggaattc ctattttcac
    46141 tttgtgttct tacggacttt gtatcttaga caggagtaga gatgagtatt cgagtaatta
    46201 ttgccggttt taagggaaag atgggccagg ctgcttgtca gatggtattg actgatccag
    46261 acttggactt ggtggcagtt ttggatcctt ttgagtctga gtcagaatgg cagggtattc
    46321 ctgttttcaa ggataaggct gatttagctg gttttgaagc ggatgtctgg gtagatttta
    46381 ctactccagc tgttgcctac gaaaatacac gttttgctct tgaaaatggc tttgctccag
    46441 tagttggaac gactggtttc acgagtgaag aaattgcaga gctaaaagaa ttttctcgtg
    46501 cccaagactt gggtggcctg attgccccta actttgcctt gggtgctgtc ttactcatgc
    46561 aatttgcgac gcaggctgcc aaatatttcc caaatgtgga gattattgag ctccatcatg
    46621 acaagaaaaa ggatgctccg agtggaacag ccattaaaac agctgagttg atggcagagg
    46681 ttcgagagtc cattcagcaa ggtgcagcag atgaggaaga gctgattgct ggtgctcgtg
    46741 gtgctgactt tgatggtatg cgcatccact cagttcgttt gccaggcttg gtagctcatc
    46801 aggaagtcat ctttggcaat cagggagaag ggttgaccct ccgtcatgac tcctatgatc
    46861 gcatctcctt catgacagga gtcaatttgg gaattaaaga agttgtcaag cgtcatgagc
    46921 ttgtctatgg attagaacac ttattatgag attaatgcaa atgccttctg aatttcagaa
    46981 ggctttacca gtattagaaa aaattaaaga agcaggcttt gaggcttatt ttgttggggg
    47041 ctctgttcga gatgcccttc tccatagccc tatccacgat gtggatattg cgacgtcttc
    47101 ttatccagaa gagaccaagc agatttttcc gcgaacagcc gatatcggaa tcgagcatgg
    47161 aaccgtcttg gtcttagatg gggatgagga gtatgaggta acaacctttc ggacagagga
    47221 tgtctatgtg gactatcgca gacccagtgc ggtttccttt gtgcgctcgc tagaagaaga
    47281 cctcaaacgc cgtgatttca cagtcaacgc ctttgccttg gatgagacag gagaaatcgt
    47341 tgacttgttc catggtttag aagatttgga aaagcaagtc ttgcgagcag ttggagtggc
    47401 tagtgagcgt ttcaacgaag atgctttacg gattatgcgt ggtttccgtt ttcaggctag
    47461 tcttggtttt gcacttgagc cagaaacatt taaagctatg aagaccttga cgccgctttt
    47521 ggagaaaatt tctgtagagc gtaccttcgt cgagtttgat aaactcttgc tggctccatt
    47581 ttggagaagg ggcttggctt ccatgattga gagtcaagct tatgactatc tccctgatat
    47641 ggcatctagc caggacaagc tcaacagact gtttgatttg gagactgatt ttacttttga
    47701 atcctctgaa caagcctggg cggctttact atgggctttg gagattgaaa atgcgcagtc
    47761 atttttgaaa tcttggaaga cctcacgcca gtttgccaag caagttcagg atttgctgat
    47821 tattttggct ctgcgtgaaa atggagaatt gagtaagcgt gattgttatc gctttgactt
    47881 ggatttactt ttacaggctg aaaatcttcg tcaagctcag ggaaaagaag tcaacccaca
    47941 agtcattaaa gaaacctacc agagcttgac tattcatgat aagaaagaaa tccaaatcaa
    48001 cggcggtatt ttgatcaagg aatatggcta tcagccaggc ccagacttgg gagagatttt
    48061 aacagagatt gagtttgcca ttgtcgatgg agaattggag aataatcgtg aagccatcca
    48121 tgcttacctg agggagaaaa aatgagtgat tttatcgttg aaaaactaag taaatccgtt
    48181 ggtgacaaga ccgtttttag ggatatttcc tttattatcc atgacttaga cagaattggt
    48241 ttaatcggtg tcaatgggac tggcaagacc acccttttgg acgtcctttc tggtgtttct
    48301 ggatttgatg gggatgtcag tcctttttca gctaaaaatg attaccagat tggttacttg
    48361 actcaggatc ctgattttga tgatagaaag acagttttgg atacggttct atctagtgaa
    48421 ctcaaggaaa tccagctcat tcgtgagtat gaattgatta tgctcgacta tagtgaggac
    48481 aagcaggcgc gtttggaacg tgtcatggca gagatggact ctctccaagc ttgggaaatt
    48541 gagagtcagg tcaagaccgt tcttagcaaa ttgggcattc aagacttatc tactcctgtt
    48601 ggggaattgt caggtggtct gagaagacgg gtacagttgg cacaagtctt acttggcaac
    48661 cacgaccttt tgcttttgga tgagccgacc aaccatctgg atattgcgat tattgagtgg
    48721 ctgaccctct ttttgaaaaa ttctaagaag accgtccttt ttatcactca cgatcgttat
    48781 ttcttagacg ctttgtcaac acggattttc gagttggagc gagcaggctt gacagaatat
    48841 cagggcaatt accaggacta tgttcgcctt aaggcagaac aggacgagcg cgatgcagct
    48901 ctacttcata aaaaagaaca actctacaaa caagaactgg cctggatgcg cagacaaccg
    48961 caggcacgtg cgaccaagca acaagctcgt atcaatcgtt tccatgatct gaaaaaggaa
    49021 gtttcaggca gtagtgctga gacagacttg actatgaact ttgaaaccag tcggattggg
    49081 aagaaagtca tcgagtttca ggatgtttcc tttgcctatg aaaataagcc cattttgcaa
    49141 aattttaatc tcttagttca ggctcaagac cgtattggaa ttgttgggga caatggtgtt
    49201 ggaaaatcaa ccctacttaa cctgattgca ggaagtcttg agccgacagc aggacaagtt
    49261 gtgattgggg aaactgttcg catcgcctat ttctctcaac aaattgaggg tttggatgaa
    49321 agcaagcgtg tgatcaatta cctgcaggaa gtggcagagg aggtcaagac cagtggtggt
    49381 tctacgactt ccatcgctga gttgctggag caattcctct tcccacgttc gacgcatggg
    49441 actttgattg agaaattgtc agggggtgag aaaaaacgtc tttatctcct caaactgctt
    49501 ttggaaaaac caaatgttct tcttttagac gagccaacca atgacctaga tattgcaact
    49561 ttgacagtct tagagaattt cttgcaaggt tttgcaggtc ccgttttaac agtcagtcac
    49621 gaccgctatt tcttggataa ggtagcgacc aagattctcg cttttgagga tggcaagatt
    49681 cgtcctttct ttggtcatta caccgactat cttgatgaaa aagcttttga aacagatatg
    49741 gccaatcaag tgcaaaaggc cgaaaaggaa aaagtggtca aggttcgaga agacaagaaa
    49801 cgcatgacct accaagaaaa gcaggagtgg gcaagtattg aaggtgatat tgaaaccttg
    49861 gaaaaacgta tcgctgctat tgaagaggaa atgcaggcta acggctctga ctttggtaag
    49921 ctggctactc tccaaaaaga attggatgag aaaaatgaag cactccttga aaaatacgaa
    49981 cgctatgagt atctcagtga atttgatagt taagactatt cgaaaatcaa agtgcaaact
    50041 atgtcggctt cacattgccg actcaagtac agcctgtgac tggcttccta atttgcactt
    50101 tgatttttat tgagtataaa agaatagaaa aatcccgtct catgaaagga tttctctatt
    50161 cttttttggt ttcttgatga aagatatttt gccaggtttc gtggcgacgc cagatactgg
    50221 tatggatgat accatctaac tcatagcaga tgagtttggt tttttgactg atggaagtaa
    50281 tttgaatgtc cttgatagca gaatttaact cttttttggt cttataagcc tctttatcca
    50341 tctgctctcc atcttggcga atatagacaa aatcatcagt caagagttct tctagttggt
    50401 ttccttggtc aatcaattgt tcacgcataa gcaatttttt atagacattg tctaaaattt
    50461 catcctcagg gataggtgct ggttcgatat ggacatcggt atcaaagacg ccaaaacgct
    50521 cctccagcat agactcgacc tgatccgcga tttcatggct ttcaaaaaca gacaagtcag
    50581 gattcatctc tagtgtaata tccaggtaga tgttgctacc gtaggtgcga cctctttgcg
    50641 atttgacctt gctgattttg ggaatttcca tgatagcctt ttggtagtcc tcgagcaggc
    50701 ggtcgtcaaa gccatctgaa agactaaagg aagactcgat gaagatatca taggcagtct
    50761 tcaagataaa gaaagtgatg atgatagcaa ccagtttatc cacaatcgga taattaaaac
    50821 tactagctag gatggcaatg gcggttccaa gtgaggtaac agcgtcagaa agattgtcct
    50881 tagcagctgc cttcagcgcc ttggagttgg atttcttact gaggcgagta ttgtagagat
    50941 agaccacaaa cataatcgct gcagaaatga ttcctagagt tgcaccaaga ggatcaatga
    51001 ccgtttcttc ccgactgaga atcttttgaa tggtatctct tagaacatcg aaaccgacat
    51061 agaacatgat gatagaagtg atcaagcttg ccaaatcttc aatcttccaa tgaccaaaac
    51121 ggtggtcacg gtctgcaggc tggcgcgcca tccgaatccc gattaagagg gctacatttc
    51181 caatgatgtc cgatacgtta ttaaaaccat cggccaccaa actggatgaa tgaaggagat
    51241 gaccagctgc taatttggct gcagacaaga tcaaataggt cgaaatactg ataatggctc
    51301 cacgctcagc taacttgaga tttgagatag attgcttcat tcaacttcct ttctagtcac
    51361 atagcattct accattatac tggttttcaa ggaaaaattc aaataaggca gtttttactc
    51421 tttgaaaact ccccaaaatt tggtataatg gtactactca agggagtagc tggcagaaac
    51481 ctgtgatagt gtcgtcattc cgaattttat gctgaaaagt atgctttccg gccctatctt
    51541 aaacagcgag acttgttatg attaacaggt ctctttttgt ttgtcgtgaa aagatatgac
    51601 agaaagagag tctgttttgc acacaatgtc aaggaggaga cacatgtcaa aagaacaaaa
    51661 acgccaagcg ttttatactc agagccctga agaggtcttg caggctgtgg atgcgaccga
    51721 gcaaggtttg tcatcaagtg aggcggaaaa gcgccttgcc gaatttgggc acaatgaact
    51781 cgaagaaggc gagaaacgat caatcttggt caaattcatc gagcaattta aggatttgat
    51841 gattatcatc ctagttgcgg cagcaatctt gtcagtcgtg acttctggtg gggaagatat
    51901 cgcagatgcc attatcatcc tagctgtggt tatcatcaac gctgcctttg gtgtttacca
    51961 agaaggaaaa gctgaagaag ctattgaagc cctcaaatcc atgtctagtc cagttgcccg
    52021 cgttcttcgt gatggacaca tggcagagat tgactctaaa gaattggtac caggcgatat
    52081 cgttgccctt gaagcaggtg acgtggtacc agcggaccta cgtttgatag aagccaactc
    52141 tcttaaaatt gaagaagcag ccttgacagg tgaatctgta ccagtcgaaa aagacttgtc
    52201 agtcgagctt gcgacagatg ctggtattgg tgaccgtgtc aacatggcct tccaaaactc
    52261 aaacgtaacc tatggtcgtg ggatgggtgt tgttgtcaat acaggtatgt acactgaagt
    52321 tggtcatatt gctggtatgc ttcaagatgc ggatgagact gatacaccac tcaaacaaaa
    52381 tttgaacaac ctttctaagg tattgaccta tgctatcttg gtcattgccc ttgttacttt
    52441 tgtagtgggt gtcttcattc aagggaaaaa tccacttggt gagttgttga cttctgttgc
    52501 ccttgccgtt gcagccattc cagaaggact tcctgctatc gttaccatcg ttctttctct
    52561 tggtactcaa gttttggcca aacgacattc catcgttcgt aagttgccag cagttgaaac
    52621 acttggttca actgaaatca tcgcttctga taagactggt acgctgacta tgaacaagat
    52681 gacagtcgaa aaagtcttct acgatgcggt tctacatgac tcagctgatg atattgaact
    52741 aggtcttgaa atgccactac ttcgttcagt tgtcttggcc aatgatacga aaatcgatgt
    52801 ggaaggtaac ttgattggtg acccaaccga aacagccttt atccaatatg ccttggacaa
    52861 gggctatgat gtcaaaggtt tcttagagaa atatcctcgt gtagctgaat tgccatttga
    52921 ctctgaccgt aagctcatgt caacagttca cccattgcca gatggtcgtt tccttgtagc
    52981 agtcaagggt gcgccagacc aactcttaaa acgttgtctt cttcgtgata aggctgggga
    53041 tattgctccg attgatgaga aggttacaaa tctcattcat acaaacaatt ctgaaatggc
    53101 tcatcaagcc ttgcgtgtcc ttgcaggtgc ttataagatt atcgatagta ttccagaaaa
    53161 tctcacttct gaagagcttg aaaatgattt aatttttact ggtttgattg ggatgattga
    53221 ccctgaacgt cctgaagccg ctgaggctgt tcgtgtggct aaggaagcgg gaatccgtcc
    53281 aattatgatt acaggtgacc accaagacac agcggaagcc attgccaaac gtttgggaat
    53341 cattgacgca aacgatacag aaggtcacgt tttaactggt gctgaactca atgaactgtc
    53401 agatgaagaa tttgaaaaag tcgttggtca atactctgtt tatgcccgtg tgtctccaga
    53461 acacaaggtt cgtatcgtca aggcttggca aaaacaaggt aaagtcgttg ccatgacagg
    53521 tgatggtgtc aatgacgcgc cagctctgaa aacagccgat atcggtatcg gtatgggaat
    53581 cactggtaca gaagtttcta agggggcttc tgacatgatt cttgcagatg ataactttgc
    53641 gactattatc gtcgcagtgg aagaaggacg taaggtcttc tcaaacattc aaaagactat
    53701 tcagtaccta ctttctgcta atactgctga agtattaacc atcttcctat caaccttgtt
    53761 tggttgggat gtcttgcagc cggttcatct tttgtggatc aacttggtaa cggatacctt
    53821 cccagctatt gctcttggtg ttgaacctgc ggagcctggt gtcatgaatc ataaaccacg
    53881 tggacgcaag gcaagcttct tctcaggtgg tgttttgagt tctatcattt atcaaggtgt
    53941 actccaagca gctcttgtta tgagtgttta tggccttgcg attgcttacc cagttcatgt
    54001 gggtgacaat catgctattc atgcagatgc cctaacgatg gcctttgcaa cccttggttt
    54061 gattcagctc ttccatgcct acaatgtcaa gtctgtctac caatccatct tgacagttgg
    54121 cccattcaag tctaagacct ttaactggtc catcttggta tcctttatcc ttctcatggc
    54181 aacaatcgtt gtagaaccgc ttgaaggaat cttccacgta accaaactag acttgtcaca
    54241 atggggaatt gttatggctg gaagcttttc aatgattatc atcgtcgaaa tcgttaagtt
    54301 tattcaacgc aaactcggtt ttgacaaaaa tgcgatttaa gggaggtttc ggatgtcagc
    54361 atatcaatta ccgaccgtat ggcaggatga agctagtaat caaggagctt ttacggggct
    54421 aaacagacca acagcaggtg cccgtttcga acaaaacttg ccaaaaggag aacaagcttt
    54481 tcagctttat tcactgagaa caccaaatgg tgtgaaggtt actatcttat tggaagaatt
    54541 actagaagct ggttttaagg aagcggctta cgacttgtat aagattgcta tcatggatgg
    54601 ggatcaattc ggatcagact ttgtgaagct caatccaaat tccaagattc cagccttatt
    54661 ggaccagtca ggtactgaag atgtaagagt ctttgagtct gctcatattc ttctttacct
    54721 tgctgagaaa tttggagcct ttttaccaag taatcctgtg gaaaaggtag aagttttgaa
    54781 ttggctattc tggcaagcag gtgcagcacc ttttctaggt gggggatttg gacatttctt
    54841 caattatgct cctgaaaaat tggaatatcc tattaaccgt tttacgatgg aagtgaaacg
    54901 ccagttggat ttattggata aggaattggc tcagaaacct tatattgcag gcaatgacta
    54961 tacgattgca gatattgcta tctggtcttg gtatggacag ttagttcaag gaaatcttta
    55021 ccaaggttct gcaaaattct tggatgcctc aagttatcaa aatctagtaa aatgggcaga
    55081 aaaaattgcc aatcgtccag ctgttaagcg tggcttggaa gtaacttata cagaaattaa
    55141 atagcaaaaa agtcatcttc ggatggcttt ttgctacagt tgagggaaag ggtttgcagt
    55201 tatcggcttt tgtgctataa ttgctataat atagtaaaaa gcaagaggag tgtgaggaag
    55261 tggaaaagaa aattgtgaag gatatcttat ttttatctca agtgtctcag ccggcaagtc
    55321 aggaggacct ttatcttgcc agagatttgc aggatacact cttagcaaat cgtgatacct
    55381 gtgttggtct agctgccaat atgattgggg tgcagaagcg cgtgattatc tttaatcttg
    55441 gcttggttcc cgtggtcatg tttaacccag tgcttctgtc ctttgaagga tcttatgagg
    55501 cagaagaagg ctgtttgtcc ttggtaggtg tgagatcaac taagcgttat gaaaccataa
    55561 ggcttgccta tcgtgacagc aagtggcagg aacagaccat taccttgaca ggcttcccag
    55621 ctcagatttg ccagcatgag ctggatcact tggaaggacg aattatttag gaggaaagca
    55681 aatgaaacga atagtctttg aacttatttt tatcgcaacg acctggtata tctttttacc
    55741 gccccttaac ctgaccagct gggaatttct cttcttcctc tgtgggcatt tgttagttgt
    55801 ggcaatctta tttggctttg gcaaggggat aaaccttgtc aaaacggttc atgtgcgcca
    55861 cggtaaggcg gaagctgcct taaatcttga gggtttcaaa atcaatcggt tagggaaaat
    55921 tctgttagct tcgattggag gaattcttct cttggcagct ttggtttcct tggtaacttc
    55981 tagcatgttt caggctaaaa attatgccaa tgtagtcacg gttacggaaa aagactttac
    56041 tgaatttcct aagagtgaca ccagtaaggt tcctatccta gatagaagta ctgctgaaaa
    56101 aattggagac cgctacttgg gttccctaac cgataaggtg tcgcaatacg tagcggcaga
    56161 tacctatacc caattgacaa ttgatgggaa accttatcgg gtcacaccac tagaatatgc
    56221 agaccctatc aaatggttta acaatcaagc caagggaatt ggtgagtata tcaaggtgga
    56281 catggtaact ggaaatgcgg atttggtgga cttgaagaca ccaatcaagt attcagactc
    56341 ggagtatttt aaccgtgatg tcaaacgtca cctgcgcttg aagtacccga ccaaaatctt
    56401 taaaactcca tcttttgagg tggacgatga gggcaatcct ttctatgtag caacggttta
    56461 ccaaaagcaa tttggacttg ctgttcctcg tcctgcttca gtcattatct tggatgctac
    56521 aaatggagaa accaaggaat acagcttatc agatgttcca gaatgggtgg acaggatcta
    56581 tccagcagag gaaaccattg agcaaattaa ctacaacggc aagtacaagg acggtttctt
    56641 gaatgccatg atttccaaga aaaacgtgac ccagactacc aagggctata attacttgtc
    56701 tatcggtaat gacatctatc tctacacagg tgtgacgtcg gctaatgcgg atgagagtaa
    56761 tcttggtttc atccttgaaa atatgcgaac aggagaaatc actaagtata gcttggcttc
    56821 tgcgacagaa gaatcagccc gtgaatcagc agaaggtgct gttcaggaga aatcctacaa
    56881 agcaaccttc ccaatcctca tcaacctcaa tgacaagcct ctctacatca tgggcttaaa
    56941 ggacaatgct ggcttggtca aagagtacgc cctggtagac gcagtcgagt accaaaatgt
    57001 tatcgttgct actacagtgg aagagatgct cagcaagtat gccaataaaa acgaccttga
    57061 aattgacaat gcaacgacag aaagcatcaa gggagtagta gcagacctca aatcagctgt
    57121 tatcaagggc gacaccgtct acttctttaa agttgatggc aagatttaca aggttaaggc
    57181 ctcagtatcc gatgaccttc cttaccttga aaatggtaga accttcgaag gtcaagtagg
    57241 aaaagacagc tatctcaaga cctttaaagt acagtaatac tctgcgaaaa tctcttcaaa
    57301 ccacgtcaac tttatctgca acctcaaagc tgtgctttga gcaacctgcg gctagtttcc
    57361 tagtttgctc tttgattttc attgagtata aaaaaggttt atttaagaaa tatatgttat
    57421 agtaaaataa attaagacta atggaggaca aagaaatggg tttttatttg atggttgctt
    57481 caatgctact cggactgctc gctttgaaga taggtttttc tcagttcaag gagaacaaag
    57541 ataaattctt atctatctta acaagtttag ctgggctagc ccttgttctc gtggctgtct
    57601 ggttaggatg gcccaaataa atagaaaaac ctcggtcaaa ccgaggtttt tcagatgaat
    57661 ttcttggttg tggctaaaaa atatgctaca ctatcaatat gaagatttta atccctacag
    57721 caaaagaaat gaatacagac ttgccgagta tcgagacaac tcttttaaga tcagaaagtc
    57781 aggctgtact tgatgccttg gctctctatt ctgccagtca attggaaagt ttctacaagg
    57841 tatcagctga gaaggctgcg gaagaatgtc aaaatatcca agctttgaaa aggcaaactg
    57901 ctcaacacta cccagccttg aagctttttg atgggcttat gtaccgtcat atcaagagag
    57961 acaaactgac cgaggtagaa caagcctatc ttgaaaatca tgtcttcatt acctcggctt
    58021 tgtacggagt cgttccagcc ttgtcgccta tggcacctca ccgtttggat tttttgatga
    58081 aattaaaagt cgctggtaag actttgaaga gccattggaa ggcagcctat gatgaagctg
    58141 tgaagcagga agaagtgatt ttctctctct tgtcatcaga gtttgagact gtattttcta
    58201 aggaaatcag agcaaagatg gtgaccttca aattcatgga ggatagaggc ggtcagctga
    58261 agattcactc aactatctcc aagaaagcgc gcggggcctt tctaacagct ttaatagaaa
    58321 atcaagtaca aactgtgggg gaagcacgtc gcttgaactt tgctggattt gtttaccgag
    58381 aagatttgtc acaaccacag ggattggttt ttgtaaagga agtatagaaa tcagataaga
    58441 gaaaagtcag cacgattatg ctgacttttt tgataactta taaagtattg gacaaatgaa
    58501 tctcatttga taaggtgtta agatgattaa ccaaattcat gattgacaga tgatttcggc
    58561 aaataaactt cttggtaatc cctgaaatat agaaattaga cacgatgata tcgtaatctg
    58621 tctgatttaa aatctcagga cttgttttta attcatccca aatatcgaag gtaaagcggt
    58681 tattacagta ataggaaagc atatcaacaa aggtaaggga tatagcatgg tcaaagttac
    58741 tgataatcaa aaccttgata ggtggtcgat tttctaacaa ctgagtagag aggttttcag
    58801 catgggttaa gatggtgtac atcaagtgtt ccaactgttc aggatggtca tgctgtccaa
    58861 ttgcctgacg gtattgagcc agttcttgac gcgcacttcc cataaagtct gggaagtaaa
    58921 cttcaaattt tttaatcgtc aatgcttttt gttcaaataa gattggggta gaaaagattt
    58981 cctgtctttc aaagaaagca gtgttgtgta aatgccaaat cagctcatcg tggttagtaa
    59041 aagtaatctt atattgtttt gataggcgtt ctaatatttg actaagtaat tggtaagagt
    59101 atcttgcttg actgttatct tctaaagagt tgaagaattc ttgaggatct aggaaaatat
    59161 cattctgaag aaaagaaata aaaatttgag cgattgtatc tggagtcatt tcaagcccaa
    59221 aatgtttaga gaaataagca agtgtctcct ggaaatttgg aatgctcttt aaaagaggat
    59281 aatattcttt gtaaaagtgg ttggggaggt caatgaagtg accattttta accctgtgga
    59341 tactgatagc tatcatcagt ttgtacattc tataaattga aaatcgcatt ggataattcg
    59401 tgattttata gaagaagtca gcaaactctg ttaaatcttc ctcaggaagg tcaggaaaag
    59461 gccaatctag gaaataatag cgctcagaaa agtattgggc aaaaaagtaa cgaatatcga
    59521 tctcatttcc acgtatctcg gatggagtaa aagataattc aatttgaaac tgagatgaaa
    59581 gaacctttgt aatatttctg cccagtcgat agaggttggc actactaaag taaagatttt
    59641 ctattgttcg ataaataggt agcccctcat tgaagaacat atactctagt aattgaaaag
    59701 attgagaatt agcaagaaaa tattgataaa tatcttctac actagtgtta acttctaaat
    59761 ctatcatgat tccgttaaca gaagattgga tgttaataga tggaaaggca atacgtaatt
    59821 catttaaatc actttttaag attcgttctg tacatcctaa tttttcggct agttcatgta
    59881 atttcatcca attacgattc tgaattaggg tttccattaa gcgtaattgt ctttgaactt
    59941 ttgtggataa aaggtttctc atacgggtat ttcctccata attcattata tctttttttg
    60001 tctaagtagt ataaaaaaat tagagcaaga aaattgtcgt tagtaatcgg aaaaattaga
    60061 ataatataag tggc
//
LOCUS       DAVKFH010000007        55209 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKFH010000007 DAVKFH010000000
VERSION     DAVKFH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.007"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..925
                     /gene="glpK"
                     /locus_tag="VW006_000427"
     CDS             <1..925
                     /gene="glpK"
                     /locus_tag="VW006_000427"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9379340.1"
                     /translation="KELKWDDEILEILNIPKAILPEVRSNSEIYGKTAPFHFYGGEVP
                     ISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGEEMQLSENNLLTTIGYGING
                     KVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYARDSHNNDEVYVVPAFTGLGAP
                     YWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDIIDTMQVDTQTAIQVLKVDGGA
                     AMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGLSVGYWKDLDELKLLNETGELF
                     EPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            967..2793
                     /gene="glpO"
                     /locus_tag="VW006_000428"
     CDS             967..2793
                     /gene="glpO"
                     /locus_tag="VW006_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9379341.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            2863..3567
                     /locus_tag="VW006_000429"
     CDS             2863..3567
                     /locus_tag="VW006_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9379342.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            3864..4067
                     /locus_tag="VW006_000430"
     CDS             3864..4067
                     /locus_tag="VW006_000430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379343.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(4154..4762)
                     /locus_tag="VW006_000431"
     CDS             complement(4154..4762)
                     /locus_tag="VW006_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379344.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            5896..7173
                     /gene="ltrA"
                     /locus_tag="VW006_000432"
     CDS             5896..7173
                     /gene="ltrA"
                     /locus_tag="VW006_000432"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9379345.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGISTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVVSEAAAKRMMYSVSRFIEKRLGLKANMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            7629..8807
                     /locus_tag="VW006_000433"
     CDS             7629..8807
                     /locus_tag="VW006_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9379346.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            9121..9252
                     /locus_tag="VW006_000434"
     CDS             9121..9252
                     /locus_tag="VW006_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9379347.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            9273..10823
                     /gene="dltA"
                     /locus_tag="VW006_000435"
     CDS             9273..10823
                     /gene="dltA"
                     /locus_tag="VW006_000435"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9379348.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNAHIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            10820..12064
                     /gene="dltB"
                     /locus_tag="VW006_000436"
     CDS             10820..12064
                     /gene="dltB"
                     /locus_tag="VW006_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9379349.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            12078..12317
                     /gene="dltC"
                     /locus_tag="VW006_000437"
     CDS             12078..12317
                     /gene="dltC"
                     /locus_tag="VW006_000437"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9379350.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            12310..13578
                     /gene="dltD"
                     /locus_tag="VW006_000438"
     CDS             12310..13578
                     /gene="dltD"
                     /locus_tag="VW006_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9379351.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLQQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            13733..14173
                     /gene="adcR"
                     /locus_tag="VW006_000439"
     CDS             13733..14173
                     /gene="adcR"
                     /locus_tag="VW006_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9379352.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            14173..14877
                     /locus_tag="VW006_000440"
     CDS             14173..14877
                     /locus_tag="VW006_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9379353.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            14870..15676
                     /locus_tag="VW006_000441"
     CDS             14870..15676
                     /locus_tag="VW006_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9379354.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAVVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            15686..17191
                     /gene="adcA"
                     /locus_tag="VW006_000442"
     CDS             15686..17191
                     /gene="adcA"
                     /locus_tag="VW006_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9379355.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYTEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(18362..19135)
                     /locus_tag="VW006_000443"
     CDS             complement(18362..19135)
                     /locus_tag="VW006_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9379356.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            19311..20714
                     /locus_tag="VW006_000444"
     CDS             19311..20714
                     /locus_tag="VW006_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9379357.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            20829..21467
                     /locus_tag="VW006_000445"
     CDS             20829..21467
                     /locus_tag="VW006_000445"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9379358.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            21479..21919
                     /locus_tag="VW006_000446"
     CDS             21479..21919
                     /locus_tag="VW006_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9379359.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            21912..22343
                     /locus_tag="VW006_000447"
     CDS             21912..22343
                     /locus_tag="VW006_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9379360.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            22334..22804
                     /locus_tag="VW006_000448"
     CDS             22334..22804
                     /locus_tag="VW006_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9379361.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            22829..23602
                     /locus_tag="VW006_000449"
     CDS             22829..23602
                     /locus_tag="VW006_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9379362.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            23599..24399
                     /locus_tag="VW006_000450"
     CDS             23599..24399
                     /locus_tag="VW006_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9379363.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            24458..26752
                     /locus_tag="VW006_000451"
     CDS             24458..26752
                     /locus_tag="VW006_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9379364.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELCIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            26762..29878
                     /locus_tag="VW006_000452"
     CDS             26762..29878
                     /locus_tag="VW006_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9379365.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            29935..31701
                     /locus_tag="VW006_000453"
     CDS             29935..31701
                     /locus_tag="VW006_000453"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9379366.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            31847..32998
                     /locus_tag="VW006_000454"
     CDS             31847..32998
                     /locus_tag="VW006_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9379367.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            33143..34027
                     /locus_tag="VW006_000455"
     CDS             33143..34027
                     /locus_tag="VW006_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9379368.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(34145..34222)
                     /locus_tag="VW006_000456"
                     /pseudo
     CDS             complement(34145..34222)
                     /locus_tag="VW006_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            34426..34677
                     /locus_tag="VW006_000457"
     CDS             34426..34677
                     /locus_tag="VW006_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9379369.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            34665..34955
                     /locus_tag="VW006_000458"
     CDS             34665..34955
                     /locus_tag="VW006_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379370.1"
                     /translation="MGALGYYEGFVPYVSNQYQNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(35360..36691)
                     /locus_tag="VW006_000459"
     CDS             complement(35360..36691)
                     /locus_tag="VW006_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9379371.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(36713..38224)
                     /locus_tag="VW006_000460"
     CDS             complement(36713..38224)
                     /locus_tag="VW006_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9379372.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(38435..39382)
                     /gene="arcC"
                     /locus_tag="VW006_000461"
     CDS             complement(38435..39382)
                     /gene="arcC"
                     /locus_tag="VW006_000461"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9379373.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(39547..40563)
                     /gene="argF"
                     /locus_tag="VW006_000462"
     CDS             complement(39547..40563)
                     /gene="argF"
                     /locus_tag="VW006_000462"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9379374.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(40621..41850)
                     /gene="arcA"
                     /locus_tag="VW006_000463"
     CDS             complement(40621..41850)
                     /gene="arcA"
                     /locus_tag="VW006_000463"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9379375.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGEKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            42606..44285
                     /locus_tag="VW006_000464"
     CDS             42606..44285
                     /locus_tag="VW006_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9379376.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYNQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQFKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQQVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLYFYLNLTVDGQLVDQLQVQVS"
     gene            complement(44325..46409)
                     /locus_tag="VW006_000465"
     CDS             complement(44325..46409)
                     /locus_tag="VW006_000465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9379377.1"
                     /translation="MKPLLETIDTRFGTASKHAFSWGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKILVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            46580..47860
                     /locus_tag="VW006_000466"
     CDS             46580..47860
                     /locus_tag="VW006_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9379378.1"
                     /translation="MIYPKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            47951..50596
                     /locus_tag="VW006_000467"
     CDS             47951..50596
                     /locus_tag="VW006_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9379379.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEVTIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            50680..51549
                     /locus_tag="VW006_000468"
     CDS             50680..51549
                     /locus_tag="VW006_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9379380.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLYRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            51543..53423
                     /locus_tag="VW006_000469"
     CDS             51543..53423
                     /locus_tag="VW006_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9379381.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVMGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            53801..54163
                     /locus_tag="VW006_000470"
     CDS             53801..54163
                     /locus_tag="VW006_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379382.1"
                     /translation="MPKTKTLAELADVILWGFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(54233..54944)
                     /locus_tag="VW006_000471"
                     /pseudo
     CDS             complement(54233..54944)
                     /locus_tag="VW006_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(54889..55071)
                     /locus_tag="VW006_000472"
     CDS             complement(54889..55071)
                     /locus_tag="VW006_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9379383.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
BASE COUNT        16762 a        10068 c        12019 g        16360 t
ORIGIN      
        1 taaagaactc aaatgggatg atgagatttt ggaaatcctt aacattccga aggctatact
       61 tccagaagtt cgttctaact ccgaaatcta cggcaagaca gctccattcc atttctacgg
      121 tggagaggtg ccaatctcag gtatggctgg ggaccaacaa gcagccctct ttggacagtt
      181 ggcttttgag ccaggtatgg ttaagaatac ttatggaaca ggctctttca tcatcatgaa
      241 tactggggaa gagatgcagt tgtctgaaaa caacctcttg acaaccattg gttacggaat
      301 caacggtaag gtttattatg ccttggaagg ttctatcttc atcgcaggaa gtgctattca
      361 gtggcttcgt gacggtcttc gcatggttga aaattcacca gaatctgaaa aatacgctcg
      421 tgattctcac aacaacgatg aagtttatgt cgttccagcc tttacaggtc taggcgctcc
      481 atactggaac caaaatgctc gtggttccgt ctttggtttg actcgtggaa caagcaaaga
      541 agactttatc aaggcgactt tgcaatctat tgcttatcaa gtgcgtgata tcatcgacac
      601 catgcaagtg gatactcaga ccgccattca agtactgaag gtggatggtg gtgcagccat
      661 gaacaacttc ctcatgcagt tccaggcgga tattttaggc attgacattg cacgtgctaa
      721 aaacttggaa acaacagctc taggagcggc cttcctagca ggtttgtcag tagggtactg
      781 gaaagacttg gacgagttga aactcttgaa cgagacagga gaactctttg agccatctat
      841 gaacgaatct cgcaaggaac aactctacaa gggctggaag aaggctgtga aagcaactca
      901 agtctttgcg gaagtagacg actaatactg gcagaataaa gcgatttatt tagaaagtgt
      961 gtaaatatgg aattttcaaa aaaaacacgt gaattgtcaa ttaaaaaaat gcaggaacgc
     1021 accctggacc tcttgattat cggtggagga atcacaggag ctggtgtagc cttgcaggcg
     1081 gcagctagcg gtcttgagac tggtttgatt gaaatgcaag actttgcaga aggaacatct
     1141 agtcgttcaa caaaattggt tcacggaggt cttcgctacc tcaaacaatt cgacgtagaa
     1201 gtggtctcag atacggtttc tgaacgtgca gtggttcaac aaatcgctcc acacattcca
     1261 aaaccagatc caatgctctt accagtttac gatgaagatg gagcaacctt tagcctcttc
     1321 cgtcttaaag tagccatgga cttgtacgac ctcttggcag gtgttagcaa cacaccaact
     1381 gcgaacaagg ttttgagcaa gaaagaagtc ttggaacgcc agccaaactt gaagaaggaa
     1441 ggcttggtag gaggtggagt gtatcttgac ttccgtaaca acgatgcgcg tctcgtgatt
     1501 gaaaacatca aacgtgccaa ccaagacggt gccctcattg ccaaccacgt gaaggcagaa
     1561 ggcttcctct ttgacgaaag tggcaagatt acaggtgttg tagctcgtga tctcttgaca
     1621 gatcaagtat ttgaaatcaa ggcccgtctg gttattaata caacaggtcc ttggagtgat
     1681 aaagtacgta atttgtctaa taagggaacg caattctcac aaatgcgccc aactaaggga
     1741 gttcacttgg tagtggattc aagcaaaatc aaggtttcac agccagttta cttcgacaca
     1801 ggtttgggtg acggtcgtat ggtctttgtt ctcccacgtg aaaacaagac ttactttggt
     1861 acaactgata cagactacac aggtgatttg gagcatccaa aagtaactca agaagatgta
     1921 gattatctac ttggcattgt caacaaccgc tttccagaat ccaacatcac cattgatgat
     1981 atcgaaagca gctgggcagg tcttcgtcca ttgattgcag ggaacagtgc ctctgactat
     2041 aatggtggaa ataacggtac catcagtgat gaaagctttg acaacttgat tgcgactgtt
     2101 gaatcttatc tctccaaaga aaaaacacgt gaagatgttg agtctgctgt cagcaagctt
     2161 gaaagtagca catctgagaa atatttggat ccatctgcag tttctcgtgg gtctagcttg
     2221 gaccgtgatg acaatggtct tttgactctt gctggcggta aaatcacaga ctaccgtaag
     2281 atggctgaag gagctatgga gcgcgtggtt gacatcctca aagcagaatt tgaccgtagc
     2341 tttaaattga tcaattctaa aacttaccct gtttcaggtg gagaattgaa cccagcaaat
     2401 gtggattcag aaatcgaagc ctttgcgcaa cttggagtat cacgtggttt ggatagcaag
     2461 gaagctcact atctggcaaa tctttacggt tcaaatgcac cgaaggtctt tgcacttgct
     2521 cacagcttgg aacaagcgcc aggactcagc ttggcagata ccttgtccct tcactatgca
     2581 atgcgcaacg agttggctct tagcccagtt gacttccttc ttcgtcgtac caaccatatg
     2641 ctctttatgc gtgatagctt ggatagcatc gttgagccag ttttggatga aatgggacga
     2701 ttctatgact ggacagaaga agaaaaagca acttaccgtg ctgatgtcga agcagctctc
     2761 gctaacaacg atttagcaga attaaaaaat taagaaaaaa taaaagaggt ggagggcagc
     2821 attccttgtc gcccgtccct tctttttaat ggagacagaa agatgatgaa tgaattattt
     2881 ggagaatttc tagggacttt aatcctgatt cttctaggaa atggtgttgt tgcaggtgtg
     2941 gttcttccta aaaccaagag caatagctca ggttggattg tgattactat gggttggggg
     3001 attgcagttg cggttgcagt ctttgtatct ggcaagctca gtccagctca tttaaatcca
     3061 gctgtgacag ttggtgtagc tttaaaaggt gacttgcctt gggcttccgt tttgccttat
     3121 atcttagccc agttcgcagg ggccatgttg ggtcagattt tggtttggtt gcaattcaaa
     3181 cctcactatg aggcagaaga aaatgcaggc aatatcctgg caaccttcag tactggacca
     3241 gccatcaagg atactgtatc aaacttgatt agcgaaatcc ttggaacctt tgttttggtg
     3301 ttgacaatct ttgctttggg tctttacgat tttcaggcag gtatcggaac ctttgcagtg
     3361 ggaactttga ttgtcggtat cggtctatca ctaggtggga caacaggtta tgccttgaac
     3421 ccagctcgtg accttggacc tcgtatcatg cacagcatct tgccaattcc aaacaaggga
     3481 gacggagact ggtcttacgc ttggattcct gttgtaggcc ctgttatcgg agcagccttg
     3541 gcagtgcttg tattctcact tttctagttt atactcttcg aaaatcaaat tcaaaccacg
     3601 tcagcgtcgc cttaccgtac tcaagtacag cttgcggcta gcttcctagt ttgctctttg
     3661 attttcattg agtattagaa aacaattatg ttgatagagc ttgggcaaga gcccaatttc
     3721 agcaaaaaat gaagtaaatc ttctcataat aaaacgcatc atatcaagca cgaaaattcc
     3781 acgaggtcaa ctacagtcag aaagctgaac aacaagccaa aacgcccaaa aaaggcggca
     3841 aaaagcaagc acctgcaagc aacgtgccga aatggtcaaa tcctgattat gtcaacgaat
     3901 tagacccaaa aatcgttgat atgctagtag aatttcacaa gtcacaaggc actttggaaa
     3961 ctcccgaggc gcaagcagaa atcgcccaaa aacgtgaaga aatcgagcaa aggagagctg
     4021 agcttgagga taaaaaacaa gagcttttga accgcttgaa caaatagagt ttcgcaagta
     4081 ttatgcttac aaattacttg agcaattaac taaaatataa accctgccta gatataaagg
     4141 cagggtttat attttagaaa ttcacgtagg ttgttacggt ttttacatac ccagtatagt
     4201 ttgagtttct atagtattca gtgataaact tccattttct ttgagcaaca tggatataag
     4261 tacttgttat gtagtatgga tatgggcttt gtgaatccaa gtaagactga taagcttgta
     4321 taccaaaata tgctccacca attattgcac cccatggacc ccccaataaa gcacctatcc
     4381 taccaatcat ataactgatt ccagcaccag tcatgaagtt agcgaatgtg ttagcttgtt
     4441 tattcccatg tattgtgttg acgtaattcc aaacattagg atcgtatgat ctaaaagata
     4501 tatttaggtc gatttcattc ttttgataag ccatataaaa tgccccattg atatagacgc
     4561 cgtcagcacg tcgttcaata gtgtctacac ttccatctgg attgacaacc tcaagaactt
     4621 catcgcttaa aatatttact tgcgtatctc cgaaccgcac tgatgagcca ttctcaaact
     4681 gagcctcacc agatacaact ttagagtttg ccgataagct atcatcagca aaaacaacaa
     4741 gcgacgggga aatgctagac atacagaaaa cagacataac tagcaaacac atgcatttaa
     4801 acatcttaga cataacggaa actcttttgt atttttgatt tttttcaact tttattatac
     4861 aataaaacca aataaaaaga aagcggtaac aatatgctta atgcgaaaat tttttatata
     4921 tttttatgtt tgatcgttat cgaaactaca ggcttgttgt tgttgaaaag aggtctcgaa
     4981 atgggttatt tagacacaga agctattatc ctcgcagttt tttcatttgc tttttacaac
     5041 ctatgttcat tcgcttgggt ctgctctaca ataaaaaata aatagacgta ttttcaaaaa
     5101 aaaaaaaatg aatatttata ttagcaaaat gacgatttaa atcgtcgttt ttttgtagta
     5161 cgacgggcat gtcgtatatc tgaggtgtaa gtcctcggtg ggcacctgct accggtgaac
     5221 ccaatagcga ttcccaagcc tgactatcgt gaggtagcgg attaaaacgg tctggggata
     5281 gaccgtttta agtctgacgc aggaaataag aatcgtcaga ggaagggata gcgaaatcgt
     5341 ggctctacga acaggaacgt gatagtaagg cgtatatagc ggataaggag gcttcaaact
     5401 ctaaagtcca aaaaggtagt cgtaacctat atgtgtaaat cacgagagta attgaattcg
     5461 gactaaggtt tgtgtgaaaa agataaatct ttctagagtc taaagactct gcgtcagatt
     5521 tcctattttc actgtaacct tttaacgtcc tcatatcttg tataaacgag gaaagatgta
     5581 cgacttatcc cgtgaggttt catgagcgct gaaagcgtag taacaacgaa tcatgagaag
     5641 tcagccgagc ccatagtagt gaggaaactt ctgtagtgga agtggagcga aggggtgaat
     5701 actcaaacag tctggggaga gactgtttga ggtctgtcgc tagaaagaga aaacgacaga
     5761 tcgaagtaat cctacttcac ttgtgtctgt aaaatgagtg gtctgataga actggacttt
     5821 gacacgtaat gactagatga aggttcacca atataagatg tccctcaggc accaaaacaa
     5881 gaaaggaata cgcacatgtc aaaactgcta gataagatat tatcacgcga aaatatgctg
     5941 gaagcctaca atcaagtaaa atccaataaa ggctcagctg ggattgatgg aatgactatc
     6001 gaagagatgg ataattatct cagacaaaac tggcgcttga ctaaggaact gataaaacag
     6061 agaaaatata agcctcaacc agttcttaaa gttgagatac ctaaaccaga cggaggcatc
     6121 cgtcaactag gaatctcaac agttatggat agaatgattc aacaggccat tgtccaagtc
     6181 atgagcccca tttgtgaacc ccatttctca gatacgagtt atggtttcag accaaatagg
     6241 tcatgtgaaa aagccatcat gaagctctta gaatacttaa atgacggcta tgagtggata
     6301 gtggatatag acctagagaa atttttcgat acagttcctc aagatagatt gatgtcctta
     6361 gtacataaca ttatcgaaga cggagatacg gaatccttga ttcgtaagta tcttcattca
     6421 ggtgttatca ttaatggtca acgttataaa acactagttg gtacaccaca gggaggaaat
     6481 ttatctcctc tcttatccaa tatcatgctt aatgaattgg acaaggaatt agaaaagagg
     6541 ggacttcgat ttgtgcgcta cgcagatgat tgtgtgatta cggtcgtaag cgaggcagcc
     6601 gctaagcgta tgatgtattc agtcagtcgt tttattgaaa aacggctagg tttgaaagca
     6661 aacatgacca agactaagat taccagacca agagagttga aatatctagg ttttgggttc
     6721 tggaaatcat cagatggttg gaaaagtcgt ccacatcaag atagtgttcg gagatttaag
     6781 cttaaattga agaaactaac acagaggaaa tggagtatag acctaacaag acgtattgag
     6841 caactgaatt tgtctattcg aggatggata aactatttct cattgggaaa tatgaaaagt
     6901 atagtcgcca gcatagatga gcgcttgcgt actcgcctac gagtgattat ctggaagcaa
     6961 tggaagaaga aatcgagacg attatgggga ttgcttaagt taggggttcc taaatggata
     7021 gcagataagg tatctggctg gggcgaccat tatcaattag tagctcagaa gtcggtactt
     7081 aaacgtgcta tatcaaaacc agtcctggaa aaacgtggac tggtttcgtg tttggattat
     7141 taccttgaac gacatgcgtt aaaagttagt tgaaccgctg tatgccgaac ggcacgtacg
     7201 gtggtgtgag aggggctaga gattatcccc tactcgatta actcccctga aatttatttt
     7261 aattatgcaa atttcacgta tttttgatgc tgagacgacg atcctgggaa cttttcagat
     7321 atttttttga ctatctaaat ctatcattag aaaagcttag agcgccaaag gatttgagcg
     7381 tttttctgat ttttaagact ttttccagtc tctttttcga ttgaagatgt aattattcta
     7441 ctaactaact aacttcttag tactagccaa caacgataat cataattcct cctaaaatta
     7501 ggaataataa aggcaatagt ttttgttttt tcatgtaaaa aacctcactt ttgttttctg
     7561 ctattttatg ctaaaatatt aaaaatcaaa tttaattcca aagtttgtaa ctaaaggggg
     7621 agcgctacat gtctaattca tttgtcaagt tgttagtctc tcaattattt gcaaatttag
     7681 cagatatttt ctttagagta acaatcattg ctaacatata cattatttca aaatcagtaa
     7741 ttgccacatc actagttcct atcttaatag gaatatcctc ttttgttgcg agtcttttag
     7801 ttccgttggt tactaaaagg ttagcgctaa atagggtttt atctttatct caatttggaa
     7861 agactatatt attggcgata ctggtaggaa tgtttaccgt aatgcaatcc gtagcgcctt
     7921 tggtgaccta tctatttgtt gttgcaattt ccatactaga tggttttgca gcacctgttt
     7981 cctatgctat tgtgccacgc tatgcgaccg atttgggtaa ggctaattca gccttatcaa
     8041 tgactggtga agctgttcaa ttgataggtt ggggattagg tggactcttg tttgcaacaa
     8101 ttggtctgtt acctaccacg tttatcattt tagtcttgta tatcatttct agctttctga
     8161 tgttatttct tcctaacgct gaagtggagg tgttagagtc agaaactaat cttgaaattt
     8221 tgctcaaagg ttggaagtta gttgctagaa atcctagatt aagacttttt gtatcagcaa
     8281 atttattgga aattttttca aatacgattt gggtttcttc cattatactt gtttttgtaa
     8341 cggagttatt aaataaaacg gaaagttact ggggatattc taatacagca tactctattg
     8401 gtattataat tagtggctta attgctttta ggctatctga aaagttcctt gctgctaaat
     8461 gggaaagtat tctttttcct cttgtagcta tggcaatagt gacactgact attctatatt
     8521 ttccaaacgc acagatgttt ttattatttt cagctttagt tggtatgtta tcgcaactaa
     8581 aagaagttcc tgaaagtgta tttcttcagg aaacagtaga ggaaaatcat ttagttaatg
     8641 tctattccgt tcttgaagtg atttctacat tagcattttc agtatttgtt ttgctaatga
     8701 gctatattac tgagagtttt ggtattagca tcagtttttg gctatcagcc atttgtctga
     8761 tgatagaagc tattttaatt tatatcagac gagattattt taaatgagag agtggttaaa
     8821 aagtctttaa aaatcagaaa agcgcatagt atcaggtgtt gaaaaaactt gatattgtgt
     8881 gttttattat gggaagattt acttcattgt ctcctgaaat ggagttttga agcaggatca
     8941 ctctatttga aaatatacat agaaaaactg tataatcctt aaaataagct gaaatctatt
     9001 ggaaacatac ggtaaacaat tgaaaagata ctatataaag tggtacaatg gtagaaaaat
     9061 aagctagtaa gaatcactct tattttagcg aaaaattaca gaaatgaatg gtttttcttg
     9121 atgaaacaga gaaaagaatt gtacctcttt cttggtcgga cagccttgta ttttcttatc
     9181 tttctagggc tgctttactt ctttagctat cttggtcagg gtcaaggaag ctttatctat
     9241 aatgaatttt aacttgaagg agaatcccta ttgtgtcaaa taaaccaata gcagatatga
     9301 ttgaaaccat tgagcatttt gctcagacac agcctagcta tcctgtctat aatgttttgg
     9361 ggcaggaaca cacttatggc gatttaaagg ctgattcgga tagtttggct gcagtcattg
     9421 accaactagg cttgcctgag aagtctcctg tggttgtttt tggtggccaa gaatatgaaa
     9481 tgttggcaac ctttgtagcg ctgactaagt caggtcatgc ctacattcca attgatagcc
     9541 attcggcctt ggagcgagtt tcagctattt tagaagtagc agagccaagc ttgattattg
     9601 ccatttcagc ctttcccttg gagcaggttt ctacaccaat gataaatcta gctcaggttc
     9661 aagaagcctt tgcccaaggg aataactatg aaatcacgca tccagtcaag ggagatgata
     9721 attactacat tatctttact tctggtacga ctggtaagcc taagggagtg cagatttcac
     9781 atgataatct cctcagcttt acaaactgga tgattacgga taaggaattt gcgacaccga
     9841 gtcgtccgca aatgctggca cagccacctt attcttttga cttgtctgtc atgtattggg
     9901 caccgacctt ggcacttggt ggtacgcttt tcactcttcc ttcagtcatc actcaggact
     9961 ttaagcaact ctttgcggct atcttttcat tgccaatcgc tatctggaca tcaacaccat
    10021 cctttgcaga tatggccatg ttgtctgaat acttcaacag tgagaaaatg cctggaatca
    10081 ctcatttcta ctttgatggt gaagaattga cggtcaaaac agctcaaaaa ctgcgcgagc
    10141 gtttcccaaa tgcccatatc atcaatgctt acggcccaac agaagcgaca gtagctctgt
    10201 cagcagttgc cgtgacagac gagatgttag cgactctcaa acgcctacca atcggctata
    10261 ccaaggctga ttctccaacc tttatcattg acgaggaagg aaataaactg ccaaatggtg
    10321 agcagggaga aatcattgtt tctgggccag ctgtttcaaa aggttatatg aacaatcctg
    10381 aaaaaacagc agaagccttc tttgagtttg aaaatctgcc agcctatcac acaggcgatg
    10441 tgggaaccat gacagatgag ggcttgcttc tctacggcgg acgcatggac ttccagatta
    10501 agttcaacgg ttaccgcatt gagttagaag atgtctctca aaacctcaac aagtctcgct
    10561 ttatcgaatc tgctgtcgca gtaccgcgct ataacaagga ccacaaggta caaaatctat
    10621 tggcttatgt catcttaaaa gacggtgttc gtgagcagtt tgagcgagat atcgatatta
    10681 ccaaggccat caaggaagac ctgacagaca tcatgatgtc ctacatgatg ccatctaaat
    10741 tcctttaccg agacagtttg ccactaactc caaatggaaa gattgacatc aaaggattga
    10801 ttaacgaggt gaataagaga tgatggagtt ttttcaacag cttcctcatt tagagccata
    10861 tggcaatcct cagtattttg tttatgtgat tgctgcaacc ttgcccatct ttataggcct
    10921 ctttttcaag aaacgctttg cctggtatga agtgttggta agtctcttct ttattgtcac
    10981 catgttggtg ggtggaaaga ccaatcaact agctgccttg ggtatttacc tttgctggga
    11041 aatattgctc ctgcttttct acaagcatta tcgaaaaagc aaggatggca agtgggtctt
    11101 ctacttagtt agttttctgt ccctacttcc gattatcttt gtcaaggtgc aaccagctat
    11161 caatggaacg cagtctttgc ttgggttctt gggaatttct tacctgacct ttcgttcggt
    11221 tgggattatc atcgagctga gagatggagt gattaaggat tttaccctct gggaattcct
    11281 ccgtttcctt ctcttcatgc caactttctc gagtggtcca atcgatcgct ttaagcgatt
    11341 taatgaaaat tatcagacca ttcctgagcg agatgagttg atggatatgc tggatgaatc
    11401 tgtccgctat atcatgcggg gctttttgta taagtttatc ctagctcatg ttttaggaga
    11461 gaccttacta cctcctctga agaatttagc cttgcagtca ggtggcttct ttaatctcta
    11521 tgccttggca gttatgtata cttttggtct ggagctcttc tttgactttg caggttattc
    11581 tatgtttgct ttggccatct caaacttgat gggaatccgt agccctatca actttaacaa
    11641 gcccttttta tcaagggatt taaaggagtt ttggaatcgc tggcatatga gtctgtcctt
    11701 ctggttccgt gactttgtct ttatgcgaat ggtgatggtg ttaaccagaa agaaagtctt
    11761 taaaaatcgt aatgtaacct caagcatggc ctacattgta aatatgctga ttatgggatt
    11821 ttggcatggc gtgacctggt actatatcgc ctatggactc tttcatggac taggtttggt
    11881 catcaatgat gcctgggttc gcaagaaaaa aacgctcaat aaggaacgga aaaaagcagg
    11941 gaaggctgcc ctacctgaga atcgctggat tcagttgctt ggcatggttg tcactttcca
    12001 tgttgtcatg ttgtcattct taatcttttc tggattcttg aataatctat ggtttaaaaa
    12061 ataaaaggaa ataaaatatg gatatcaaat cagaagttat cgaaattatt gatgagttgt
    12121 ttatggaaga tgtttctgac atgatggatg aagatctttt tgatgcaggt gtcttggata
    12181 gtatgggaac ggttgagttg attgtggaga ttgagaaccg ttttgacatt cgtgtccctg
    12241 taacagagtt tggtcgtgac gactggaata cagctaataa aattatagct ggtattgtgg
    12301 agctacaaaa tgcttaaacg cttatggatg atcttcggac cggtcttgat cgctggtttg
    12361 ttggtttttc tgctcatttt cttttatcct actgagatgc atcataatct aggagctgaa
    12421 aagcgttcag cagtggctac tactatcgat agttttaagg agcgaagtca aaaagtcaga
    12481 gcactatctg atccaaatgt gcgttttgtt cccttctttg gctctagtga atggcttcgt
    12541 tttgacggtg ctcatcctgc ggtattagct gagaaataca atcgttccta ccgtccttat
    12601 cttttaggac aggggggagc tgcatcgctt aaccaatatt ttggaatgca acagatgtta
    12661 caacagctgg agaataaaca agttgtgtat gttatctcac ctcagtggtt cagtaaaaat
    12721 ggctatgatc cagcagcctt ccagcagtat tttaatggag accagttgac tagttttctg
    12781 aaacatcaat ctggggatca ggctagtcaa tatgcagcga ctcgcttact gcaacagttc
    12841 ccaaacgtag ctatgaagga cctggttcag aagttggcaa gtaaagaaga attgtcgaca
    12901 gcagacaatg aaatgattga attattggct cgttttaatg aacgccaggc ctcctttttt
    12961 ggtcagtttt cggttagagg ctatgttaac tacgataagc atgtagctaa gtatttaaaa
    13021 atcttgccag accagttttc ttatcaagca atagaagatg ttgtcaaagc agatgctgaa
    13081 aaaaatactt ccaataatga gatgggaatg gaaaattatt tctataatga gcagatcaag
    13141 aaggatttga agaaattaaa ggattctcag aaaagcttta cctatctcaa gtcgccagag
    13201 tataatgact tgcagttggt tttaacacag ttttctaaat ctaaggtaaa cccgattttt
    13261 atcattccac ctgttaataa aaaatggatg gactatgctg gtctacgaga ggatatgtac
    13321 caacaaacgg tgcagaagat tcgctaccag ttagaaagtc aaggttttac caatatagca
    13381 gatttttcta aggacggcgg ggagcctttc tttatgaagg acaccattca ccttggttgg
    13441 ttgggttggt tggcttttga caaggcagtt gatcctttcc tatccaatcc cacaccagct
    13501 ccgacttacc atctgaatga gcgctttttc agcaaagatt gggcgactta tgatggagat
    13561 gtcaaagaat ttcaatagat aatataatat agaagagttc aagaatgaaa ctagttttca
    13621 cgttttttct tgactctttt tttgcttgtg atataattaa ctggtaaaca aaatgtcaaa
    13681 gaatagtatt tttctcttaa aaaagagaag tctaaaagga aaggagtcag atatgagaca
    13741 gctagcaaag gatatcaatg cttttttgaa tgaggtgatt ttgcaggcgg aaaatcagca
    13801 tgaaatccta ataggtcatt gcactagcga ggtggccctg accaatactc aggagcatat
    13861 ccttatgctc ttgtcagagg aatctttaac aaattcagaa ttggctcgtc gtctcaatgt
    13921 cagtcaggcg gcagttacca aggccattaa gtctttggtc aaggaaggga tgttggaaac
    13981 atctaaagat tctaaagatg cgcgtgtgat tttttatcag ttgactgact tggctcgtcc
    14041 aatcgctgag gagcaccacc atcaccatga gcatacactt ttaacctatg aacaagtggc
    14101 gactcagttt actccaaatg aacaaaaagt gattcagcgg tttttgactg ctttagtagg
    14161 agaaatcaaa taatgagata tattacggta gaggatttgt ccttctatta tgataaggag
    14221 cctgttcttg aacatatcaa ttattgtgtt gatagtgggg aatttgttac cttgactggg
    14281 gaaaatggag cggctaagac gacgctcatc aaggctagtc ttggaattct gcaaccacgc
    14341 attggaaagg tggctatttc aaagacaaat acgcaaggta agaaattgag aatagcctat
    14401 cttcctcaac aaattgccag ttttaatgct ggttttccaa gtacggtcta tgaatttgtc
    14461 aagtcgggtc gctatccgag aaaaggctgg ttccgtcgtt tgaatgctca tgatgaggag
    14521 catatcaagg ctagtctgga ctcagttggc atgtgggaac atcgagacaa acgcttgggg
    14581 tctctatctg ggggacaaaa gcagcgagcg gtaattgcgc gtatgtttgc ttctgaccct
    14641 gatgtgttta tcctagacga gccgacaacg gggatggatg caggaagtaa aaatgaattt
    14701 tacgaactca tgcaccacag cgcccatcat catggcaagg ctgttttgat gattacccat
    14761 gaccctgaag aagttaagga ttatgcggat cgcaatattc atctagtccg taaccaagac
    14821 tcgccatggc gttgtttcaa tgttcatgag aatggccagg aggtgggcca tgcttagttt
    14881 attatcttac gactttatac agcgcgcctt tttggcggtt attgctatga gtcttttctc
    14941 accggtattg ggaaccttcc tcatcttgcg tcgtcagagt ttgatgagtg atacccttag
    15001 ccacgtctca ctttcaggtg tagcctttgg tctggttttg gggatttctc caactgtttc
    15061 tactattgcc attgtcttga ttgcggcggt ctttctggag tatctccgta cggtttacaa
    15121 gagctttatg gaaatcggga cagctatcct catgtcaaca ggtctggctg tttctctgat
    15181 tgtcatgagc aagggtaaaa gctcgagttc aatgagtttg gaccaatatc tctttggttc
    15241 gatcgtgact atcagtgaag aacaggtcat ttccctcttt gtcattgcgg tggttgtttt
    15301 gattttgacc tttctctttc ttcgtcctat gtatatctta acttttgacg aagatacggc
    15361 ctttgtggat ggcttgccag ttcgtaccat gtccattctt tttaacatgg tgacaggggt
    15421 ggctattgcc cttatgattc ctgcagcagg agctcttctg gtatcgacca ttatggtctt
    15481 gccagctagt attgccctgc gtctggggaa aaactttaaa tcggttatgc tgcttgccag
    15541 tgcgattggc tttttgggaa tggtagcagg actttacatt tcctactatg cagaaacacc
    15601 tgcaagtgca agtattacca ttatttttgt aactgtcttt atactaatca gtttagtaag
    15661 acgttttatc aaataggaga cgaacgtgaa aaaaattagc ttattactag ccagtctatg
    15721 tgccttgttt ttagtggctt gttccaatca aaaacaggca gatggtaaac tcaatatcgt
    15781 gacaaccttt taccctgtct atgaatttac caagcaagtc gcaggagata cggctaatgt
    15841 agaactccta atcggtgctg ggacagaacc tcatgaatac gaaccatctg ccaaggcagt
    15901 tgccaaaatc caagatgcag ataccttcgt ttatgaaaat gaaaacatgg aaacatgggt
    15961 acctaaattg ctagatacct tggataagaa aaaagtgaaa accatcaagg cgacaggcga
    16021 tatgttgctc ttgccaggtg gcgaggaaga agagggagac catgaccatg gagaagaagg
    16081 tcatcaccat gagtttgacc cccatgtttg gttatcacca gttcgtgcca ttaaactagt
    16141 agagcacatc cgcgacagct tgtcagcaga ttatcctgat aaaaaagaga cctttgagaa
    16201 gaatgcagct gcctatatcg aaaaattgca agccttggat aaggcttaca cagaaggctt
    16261 gtctcaagca aaacaaaaga gctttgtgac tcaacacgca gcctttaact atcttgcctt
    16321 ggactatgga ctcaaacaag tcgcaatctc aggcctttct ccagatgcag agccatcagc
    16381 tgctcgcttg gcagaattga cagagtatgt caagaaaaat aaaatcgcct atatctattt
    16441 tgaagaaaat gcctcacaag cccttgctaa cacactttca aaagaagcag gtgtcaaaac
    16501 tgatgtcctc aatcctttag aaagtctgac agaagaggac accaaggctg gagaaaacta
    16561 catttccgtg atggagaaaa acctcaaggc tttgaaacaa acaacagacc aagaaggccc
    16621 agcaatcgaa cctgaaaagg cagaggatac caagacagtc caaaatggtt acttcgagga
    16681 tgcagctgtc aaggaccgca ccttgagtga ctatgcaggt aactggcaat cagtttatcc
    16741 tttccttgaa gacggcacgt ttgaccaagt ctttgactac aaggctaagt tgactggtaa
    16801 gatgacccag gctgagtaca aggcttacta tacaaaaggc tatcagacag atgtgactaa
    16861 gattaacatt actgataata ctatggaatt tgttcaaggt ggacaaagca agaaatacac
    16921 ttacaagtat gtcggtaaga aaattttgac ttacaagaaa ggcaatcgtg gcgtgcgttt
    16981 cctctttgaa gccacagatg ctgacgctgg acaattcaaa tatgttcagt ttagtgacca
    17041 caatgttgcc ccagttaagg cagaacattt ccatatcttt tttggaggta caagccaaga
    17101 agctctattt gaagaaatgg ataattggcc aacctactac ccagataacc tatctggcca
    17161 agaaatcgcc caagaaatgt tggcgcattg atgagaaatc gactagttca taagagctag
    17221 tcggtttttt gatactagat ggctaacttc attatagacc tttcagaact aagggaacct
    17281 gaaaaaataa ataaaactct tgaaatatct ggacttatat gctagaatgt agttagtgta
    17341 taaggttcat tagaacacca aagcccctaa ctagtgccat agttaagggc ttttttgacg
    17401 tatcttagcg atttaatggg tgtttatagg ctagtcagtc ggtctacttc ttaccatcta
    17461 gccatttgca gacgaaatac aagataattc cagctataac atctgctata atagtaagag
    17521 caagctctgg tataaggctc attagtttca cctcctctca cgaacccata ggaacgtaat
    17581 cggtaaccga tgacaaaagt attatatcac actaaattta gatcatcaac ctcacttaat
    17641 gcttgaacta ttgtagatta tatgctaaaa tgtagttaat tgataaggtt cattagaaca
    17701 ccaaagcccc taactatggc cgtagttaag ggcttttttg acgtatctta gcgatttaac
    17761 gggtgtttat aggctagtca ctcggtctac ttcttactat ctagccatct gcagacgaaa
    17821 tacaagataa ttccagctat aacatccgct ataatagtaa gagcgagctc tatgataagg
    17881 ctcattagtt tcacctcctc tcacgaaccc ataggaacgt aatcggtaac cgatgacaaa
    17941 aatagtatac cacaatacat ttagatcatc aaggtcactt aattcttgaa atatagggtt
    18001 taggcgacaa agtctttaaa atcagaaaaa cgcataatat caggtgtgca aaaacttgat
    18061 actatgcgtt ttattgtggg aaggtttact ccattttctc ctgaaattga gtttttgtcc
    18121 agcctctgtt tttagggttg ctgagaaaat aatgtcatgt ggtgaatatt tgtaaatcag
    18181 tcagcagaca gaacgaatac tcttcgaaaa tctcttcaca tcatgtcagc ttcgtctttc
    18241 cgtatatatg tgactgactt catcagttct atctacaacc tcaaaacagt gtttcgagct
    18301 gactttatca gttttcaaat ctgtactttg agcaagctga gactagcttc ctatttgatt
    18361 ttcattgaat atcagaaacc cattctccat caaataattc gactgcgtct aataattttt
    18421 gatctggcac ggtgtctgaa ataaaggttg tgtatttgga gaggggatta attttaaaaa
    18481 atccagtctt gtaaaattta gaactatcaa tcagtaagat ggtttcatgg gctttgtcaa
    18541 taatattctt ttttgaaata gcttggctga gagaagcttc ataaacatat tggtcatcaa
    18601 tacctcttgc tgaacaaaat gctaaatcga tattaaaatg atctaataaa gaattttcct
    18661 tatcatagtt gaccacggaa caggattgat gtttgacctc gccagatgtg ataaagattt
    18721 tggagctatc tttaacagtt tcagataggg tttgtgcagt atgtaaacca tttgtaaaaa
    18781 taatcaaatt atcaagttca gaaagatagg gacaaagttc gtagacagta gtactggaat
    18841 ccagataaac acacatacca gaccgaataa agtctttagc gagactagcg attagtcttt
    18901 tttgcctagt actttctcct tcccgtattt gatgagaaag ttcaattgtg ttcatagagg
    18961 acagggtcac gtatccgtgc tttcttttga taagaccttg attttctaag aaaattaaat
    19021 cacgacgtaa ggtacttgtg ctggagaaag tgatttctgc cagctctttt acggcaattc
    19081 tttttttctt tttaataatt tcaatcaatt caagtacacg ttcatctttt atcataagct
    19141 cctcctaatt tatcatttca actatattat agcacaaatt ggaggaattt gaattatttt
    19201 tatgaatatt gggttaacat ttgaacatta ttcaagtaag cgttcacata ttgaaaaaat
    19261 aaaatgtggg gattataata aagttaatca aggacgaaga gagaagaaaa atggaagcgg
    19321 ttttagcaat agatttaggt gcgacttctg gaagagcaat cgttggttac ctttctgaaa
    19381 ataaactagt aatggaagaa ataaatcgct tttctaatct acctattaga gtaaaagggc
    19441 atttatcttg ggatattgac tttctactag ctaaaattct tgaaagtatc cgcttggcta
    19501 atactagtta caagatttta tctatcggta ttgacacatg gggagttgat tttggactga
    19561 ttgataatga aggtaagctg ttatcacaac ctgttcatta tcgtgatgaa agaacaaagg
    19621 gagtgttaaa ggaaatatct gaaatgactg aattagaaaa actgtattca gagacaggaa
    19681 atcagattat ggagataaat accttgtttc aactctttaa ggcacgtcaa gaatctcctg
    19741 actctttcta taagaccaat aagattcttt taatgccaga tttgtttaat tatctcttga
    19801 caggtaagtt tgctacagaa aaaagcattg cttcaacaac tcaattattt gatcctagga
    19861 gtcaaaattg gaatcagaat atcttaaaac tatttgaatt ggattcatct ttacttcctg
    19921 aaattgtttc agagggaaat gttcttggaa ggataaaaaa ggagtatggt ttaggcgata
    19981 ttcctgttgt gaatgtttgt agtcatgata cagcaagtgc gattgtctca gtacctaaga
    20041 cagaaggtag tttatttatt tcatcaggta cttggtcttt ggttggagtg gaacttactt
    20101 caccgattct tactaccgaa tccttcagtt atggatttac aaatgaagtc ggtaaagatg
    20161 gagagattac atttctgaag aattgtacag ggttgtggat catagaggaa ctaagacgtt
    20221 catttgaacg aagagggaaa gcctattctt ttgatgatat taggacaatg gtggagaaag
    20281 aaaaagaaaa tcttcctctg attgatactg aatcaactga atttgcaaca gaatctgata
    20341 tgcacaagac tttgacagaa tatctagctt atcatcatga aactagagag tggacagatg
    20401 gacaactatt taagattgtt tatgaaagcc tagctgaaac gtataggaaa gcgatagagt
    20461 tactagaaga actaactcat aaggtttata agaggatata tgtgattgga ggaggtgcta
    20521 gagccagtta ctttaaccaa atgattgctg atagaactgg taaagaggtt cttacaggtt
    20581 cgactgaggg tacagctgtg gggaatattg ttgtgcagct catagctatg ggacaattaa
    20641 aagggatgga agaagctcac catgttattg aggagtttct acaattagag agttattact
    20701 cccaaaagaa ttaaaaagat tgagagtttg taaatttgcc tccctccccc ttcttaagtt
    20761 ttgtacaaga aggggggata attggtgaat tgaaaaatat ttagtgtttt gatatgagga
    20821 ggacaagaat gtcagatgta aaacaagaat taattaagta tggcaagaag ctagtagaaa
    20881 cagatttgac gaaaggaaca ggtgggaatc tcagtgtttt cgatcgtgaa aaacaattga
    20941 tggcaattac cccgtcgggt attgatttct ttgaaatcaa agaatccgat attgtagtga
    21001 tggatattaa tggaaatgtt gtagagggag aacgcttgcc atctagcgaa tggtatatgc
    21061 atttgattca atatcaaact cgtgatgata tcgatgcaat tatccatgct catacaactt
    21121 atgcaacagt attagcttgt ctcagagaac cacttccggc tagtcattat atgattgcag
    21181 tggcagggaa agatgttcgg gtagctgagt atgcaacata tggtacgaaa gaattggctg
    21241 tgaatgcagc taaagcaatg gaaggtcgta gagcagtttt actagcgaat catggaattt
    21301 tagcaggtgc acaaaattta ttgaatgcat ttaatattgt tgaagaagtt gaatattgtg
    21361 caaaaattta ttgtttagct aagaattttg gagagccagt agttcttcct gatgaggaga
    21421 tggatttaat ggcagaaaaa tttaaaacat acggtcagag aaaataggga ggatattaat
    21481 gttaaaacat ataccgaaaa atatttctcc agatttattg aagactttaa tggaaatggg
    21541 acatggagat gaaatagtat tagctgacgc gaattatcct tctgcctcat gtgcaaataa
    21601 gctaattcgt tgtgatggcg taaatattcc agaattatta gattccattc tgtatttaat
    21661 gccattagat agttacgtcg atagttcaat tcagtttatg aacgttgttt cgggtgatga
    21721 tattcctaag atatggggta cctatagaca gatgattgaa ggtcatggta cagatcttaa
    21781 aacgattact tatcttagaa gagaagactt ttatgaacgt agtaagaaag cttatgctat
    21841 tgttgctaca ggagaaactt cactttatgc taatattatc cttaagaaag gagtagttgt
    21901 tgaaagagaa aatgtttaat agaggaattt tagttgccag tcatggtaat tttgctagcg
    21961 gagctctcat gaccgcagaa atgtttgttg gtgagacaac aaatgataga gttaggacat
    22021 taggtttgat gcctggagag aatattgtag agtttgaaca ttattttaaa aatcaagtaa
    22081 atgaactgtt agactcaaat caagaggtta tcgttttgac tgacttgatt ggaggaagtc
    22141 ctaataatgt ggctttgtca cggtttttaa atttggattc agttgatatt gtaacagggt
    22201 ttaatatccc tctcctagtg gaattaatat caagttatga ttcaaaaatc aatttagaag
    22261 aaattgttca aaatgctcaa aatagtttgt ttaatgttaa acaacaactt aacgtagagg
    22321 aggaagagga tttatgtcta tagagtttgt tcgtattgat gaccgtctgg tacatggtca
    22381 agttgtcact acgtggctaa aaaagtatga tattgagcaa gttatcattg ttaatgatcg
    22441 catctcagaa gataaaacac gacaatctat tttaaagatt tctgcaccgg taggtttaaa
    22501 aattgttttc tttagtgtaa aacggtttgt ggaagtttta aactctgtgc caataaaaaa
    22561 gagaacaatg ctgatatata caaatccaaa agatgtgtat gattctattg aaggaaattt
    22621 aaaattggag tacctcaatg taggacagat gagtaaaacg gaggaaaatg aaaaggtaac
    22681 gggaggtgta gctctaggtg aagaagacaa atattatttt aagaaaatag ttgataaggg
    22741 aacgagagtt gaaattcaaa tggttcctaa tgataaagtt acaatgttgg aaaaattttt
    22801 ataaaaataa tttaaggagg tacagtatat gctattcaca caagcattac tggtgacatt
    22861 agttgggatt attgccacta ttgactataa tggaccgtta tttatgattc accgtccgtt
    22921 agttacaagt gcaatggttg gcttagtctt aggagatttc acccaaggtg ttcttattgg
    22981 ttcagctctt gaattaactt ggctcggtgt aacaggtatt ggaggttaca ctccaccaga
    23041 tactatttca ggtgcgatta ttggtactgc atttggtatt ttgtctggtc aaggagaaac
    23101 tgctggtatc gctatagcag ttccaattgc agttgctact caacagttgg atgttcttgc
    23161 aaaaacttta gatgtttatt ttgtgaaaaa agctgataat gatgctaaaa acggagatta
    23221 ttcaaagatc ggtttttatc attattcaag tttggtttta atcactttat ttaaaattgt
    23281 accaattttc ctagctatta tgcttggagg ggaatatgtg gcagacttgt ttgctaaggt
    23341 tccaccaatc gttatgcagg gacttaactc tgcaggtgct ttactacctt caattggttt
    23401 tggtatgctt ttaaatatga tgctcaagaa aaatatgtgg gtattcttgt tgattggatt
    23461 catttgttct gtgtatggag gaatgtcaac cattgggatc tcactagttg gtattgcggt
    23521 agcatacttc tacgatatga ttggaagtaa accacaagaa acaacttcaa gtagtgatgt
    23581 tgaggaggat cttgatctat gatgaataat aaagtaacta aagttgaact taaaaaagtt
    23641 ttcaaacgaa gttttatgta tggttcttca tggaactatg agagaatgca gaacctaggt
    23701 tttctatata caattcttcc agtattgaaa aaactatacc cagacaaaga ttcagcttct
    23761 cctgcaatga aacgtcacct tgagtttttc aatactcatc aaacagcggc accatttatt
    23821 cttggagtta cttccgctat ggaagaacaa gaaggaaatg aaggtgcagc ttcaattact
    23881 ggtattaaag ttggcttgat ggggccactg gctggtctag gagatagttt gttctggctg
    23941 acactagttc ctatctgttt tagtattggt gcgtcttatt ctaaagacgg cggtgcttta
    24001 ggtatcttta tcgccttaat attgtttaat attattaata ttcctgttaa atatttcggt
    24061 ttgaaatatg ggtatactaa gggttctagt cttatccaag aaaataatac aaaaggaaca
    24121 ttgaatcgcg ttacgagtat ggcgacagca ttagggctag tactagtggg tggtttgatt
    24181 ccatcaatgg ttggtattaa ttttggatta gaatttaagc agggggaact tgttatttct
    24241 gttcaagaaa tgattacaaa attaattcca ggatttatcc ctatggcttt gactttatta
    24301 atgtgtaaat taattagaaa aggaaagaat ccggttgtac taatctttag tgttatggct
    24361 attggagtta tcctagttgt tttaggaatt ttgaagtagt agaaagtgtg gaggtggtat
    24421 ttgggatatc acctccattt tggaagagag gtaaagagtg aaattatggt ataagaaagc
    24481 tgccgcaaat tggaatgaag ccttgccgat tgggaacggt catttaggtg gtatgattta
    24541 tggttcagct acaaaagaat gtattcaact aaacgatgag actatttggt atagaggaaa
    24601 gtcagataga aataatccag actcactatt gcatcttaaa aaaattcggg aatatctttt
    24661 agatggagaa attcagaaag ccgaagaatt gataaagtta acaatgtttg ctaccccaag
    24721 agatcaaagc cactatgaat tacttgggga actttgcatt gagcatatag atattcagtc
    24781 ttgtgctctt tcattgtatg aaagagagct agatttagat acagctattt ctaatgttgt
    24841 gtttgagcct aatagttgta atttacaaat aaaaagagaa tattttacga gttttaataa
    24901 gaatatttta tgttgccgta tagtgtcatc agttcaaaac acattaaatt taaacattaa
    24961 tttgggtaga aataaacggt ttaatgacga agtatctaaa ctggattcaa gtacaatttt
    25021 aatgtcggcc tctgctggag gtagaaaagg tgttcagttt aaagtagtat gtcattctaa
    25081 ggttacggat ggtgaagtaa gtgtattggg agagacaata gttattcgga atgctacaga
    25141 ggtatttctt tatctcaaat caatgacgga ttattgggga aatatagata tttcttctct
    25201 tcagggagaa tttagtagta ttgattactt tacagaaaaa gatgaacatg taaaaaaata
    25261 tcaggagcaa tttaatagag ttgattttaa actagactat agtaaaggtt gtcttagcat
    25321 tccaacgaat ctacttcttg aaaacactaa aaagtatagt aactacttga ctaacttgtt
    25381 atttcattat ggaagatatc tgttaatatc gtctagtcaa ccgaatggtt tacctgccaa
    25441 tcttcaagga atatggtgtg atgaattaaa tccaatttgg ggttctaaat atacgattaa
    25501 tattaatact caaatgaatt attggatggt aggtccatgt gatttaccag aagtagaata
    25561 tccattattt gatatgctcg aaagaatgag agaaccggga agactaaccg ctaagaaaat
    25621 gtatggagct agaggtttta cagcacatca taatacggat ggttttggcg atacggctcc
    25681 ccaatctcat gccatggggg ctgcaatttg ggtattaact attccatggt tatgtactca
    25741 tatttgggaa cactatttat atttccaaga tgagcgtatt cttacggaac attttgaaat
    25801 gataaaagaa gcatttcttt tctttgaaga ttatttattt gaggtggatg gctacttgat
    25861 gacaggtcca agtgtctcac cggaaaataa atatcgctta aaaaatggta ttgaaggaaa
    25921 tgcttgtcta tcatctacaa ttgataatca aattctaaga tatttttgtg attcatgcat
    25981 tggcattgca aaacaattag gagacaattc ggattttatt agtcgtgtga aggagttaaa
    26041 aaagaaatta cctagaacaa aaataggtag taatgggcaa atacaagaat ggctagagga
    26101 ttatgaagaa gtagagcctg gtcatagaca catttcacct ctatttgggc tttatcctta
    26161 taatgagatt gatattcata aaactccgga attagcagaa gcagctaaaa tcactatcaa
    26221 taggagatta tcaaacgcta actttttatc ttcacaggat agggagcaag cgattaataa
    26281 ttggttagta agtggtttgc atgctagtac acaaacaggt tggagtgctg catggctgat
    26341 tcattttttt gcgagactat atcaaggtga acctgcttat aaccagatta atggtttgtt
    26401 aaataatgcg actcttggca atttatttct tgaccatcca ccatttcaaa ttgatggtaa
    26461 tttaggtttg gtgagtggaa tttgtgaatt attagtacag agccatcata attggttatc
    26521 actaattcca gctttacctt ctgcttggtc agaaggagaa gtgaaaggtt tcagagtaag
    26581 aggaggatat aaggtatcgt ttgcttggaa aaatggggat ataacattcc taaaattgga
    26641 aggaggaaac aaagatcaaa aagtaagagt aagaatatat ggcaaaaata ctgatgtaca
    26701 aaatattgaa ttggtattta attcagaaaa aattattgag ttaaattttt aggtataagt
    26761 catgaataaa gaaaaaataa aaagaaaatt aatcacaata ttgtttgtat gtattgggat
    26821 gttatgtttt ggattgttag caggagttaa ggctgataat cgtgttcaaa tgagaacgac
    26881 gattaataat gaatcgccat tgttgctttc tccgttgtat ggcaatgata atggtaacgg
    26941 attatggtgg gggaacacat tgaagggagc atgggaagct attcctgaag atgtaaagcc
    27001 atatgcagcg attgaacttc atcctgcaaa agtctgtaaa ccaacaagtt gtattccacg
    27061 agatacgaaa gaattgagag aatggtatgt caagatgttg gaggaagctc aaagtctaaa
    27121 cattccagtt ttcttggtta ttatgtcggc tggagagcgt aatacagttc ctccagagtg
    27181 gttagatgaa caattccaaa agtatagtgt gttaaaaggt gttttaaata ttgagaatta
    27241 ttggatttac aataaccagt tagctccgca tagtgctaaa tatttggaag tttgtgccaa
    27301 atatggagcg cattttatct ggcatgatca tgaaaaatgg ttctgggaaa ctattatgaa
    27361 tgatccgaca ttctttgaag cgagtcaaaa atatcataaa aatttggtgt tggcaactaa
    27421 aaatacgcca ataagagatg atgcgggtac agattctatc gttagtggat tttggttgag
    27481 tggcttatgt gataactggg gctcatcaac agatacatgg aaatggtggg aaaaacatta
    27541 tacaaacaca tttgaaactg gaagagctag ggatatgaga tcctatgcat cggaaccaga
    27601 atcaatgatt gctatggaaa tgatgaatgt atatactggg ggaggcacag tttataattt
    27661 tgaatgtgcc gcgtatacat ttatgacaaa tgatgtacca actccagcat ttactaaagg
    27721 tattattcct ttctttagac atgctataca aaatccagct ccaagtaagg aagaagttgt
    27781 aaatagaaca aaagctgtat tttggaatgg agaaggtagg attagttcat taaacggatt
    27841 ttatcaagga ctttattcga atgatgaaac aatgccttta tataataatg ggagatatca
    27901 tattcttcct gtaatacatg agaaaattga taaggaaaag atttcatcta tattccctaa
    27961 tgcaaaaatt ttgactaaaa atagtgagga attgtctagt aaagtcaact atttaaactc
    28021 gctttatcca aaactttatg aaggagatgg gtatgctcag cgtgtaggta attcctggta
    28081 tatttataat agtaatgcta atatcaataa aaatcagcaa gtaatgttgc ctatgtatac
    28141 taataataca aagtcgttat cgttagattt gacgccacat acttacgctg ttgttaaaga
    28201 aaatccaaat aatttacata ttttattgaa caattacagg acagataaga cagctatgtg
    28261 ggcattatca ggaaattttg atgcatcaaa aagttggaag aaagaagaat tagagttagc
    28321 gaactggata agcaaaaatt attccatcaa tcctgtagat aatgacttta ggacaacaac
    28381 acttacatta aaagggcata ctggtcataa acctcagata aatataagtg gcgataaaaa
    28441 tcattatact tatacagaaa attgggatga gaatacccat gtttatacca ttacggttaa
    28501 tcataatgga atggtagaga tgtctataaa tactgaaggg acaggcccag tcccttttcc
    28561 aacaccagat aaatttaatg atggtaattt gaatatagca tatgcaaaac caacaacaca
    28621 aagttctgta gattacaatg gagaccctaa tagagctgtg gatggtaaca gaaatggtaa
    28681 ttttaactct ggttcggtaa cacacactag ggcagataat ccctcttggt gggaagtcga
    28741 tttgaaaaaa atggataaag ttgggcttgt taaaatttat aatcgcacag atgctgagac
    28801 tcaacgtcta tctaattttg atgtgattct atatgacaat aatagaaacg aagttgctaa
    28861 gaaacatgtt aataatttgt cgggtgaatc tgttagtcta gatttcaaag aaaaaggagc
    28921 aaggtatatt aaagttaaat tactaacgag tggagtgcct ttgagtttag cagaagtaga
    28981 ggtttttaga gaatcagatg gtaagcaatc tgaagaggat atagataaaa taacagaaga
    29041 taaagtagtc tctacaaata aggtagctac tcaaagttca accaattatg agggtgtagc
    29101 tgctttagca gttgatggta ataaagatgg agattacgga catcattcgg tgactcatac
    29161 taaggcagat tctaacgctt ggtggcaggt cgatctggga gaagagttta cggtttctaa
    29221 agttgatatt tataatagaa cagatgccga acctcagcgt ttatctaatt ttgatgttat
    29281 ttttctatct tcatcaggag aagaagtttt tagaagacat tttgataaag tagttgatgg
    29341 tttgttatct ttaaaagtac cttctgtagg ggctaagcta gtcaaaatag aattaaaatc
    29401 agcagctatt ccgttaagtt tagcggaagt tgaagtctat ggttcaaaga gaactccgaa
    29461 gaaactttct aatattgcat taaccaaaga aactcgacag agttcaacgg attacaatgg
    29521 tttttctcgt ctagcagttg atggaaataa aaacggagat tatggtcatc attcagtgac
    29581 tcataccaaa ggagattctc cttcatggtg ggagatagat ttagcacaaa ccgaagaatt
    29641 agaaaagtta attatttata atagaacaga tgctgaaatt cagagattat caaattttga
    29701 tattattata tatgattcaa ataatcatga agtttttaaa caacatattg acagtttaga
    29761 aagcaataat ctatccatag acttaaaagg gctgaaggga aaaaaggtta gaatttcttt
    29821 gagaaacgca ggaattcctt taagtttggc agaggtagag gtttatactt ataagtaatt
    29881 ttaaaaatta tcatcggtag tatgaaagaa acagaaaaat aagaggaaaa tagtatgatt
    29941 caacatccac gtattggtat tcgtccgact attgatggtc gtcgtcaagg tgtacgcgaa
    30001 tcacttgaag tacaaacaat gaacatggct aaaagtgtgg cagatttgat ttcaagcaca
    30061 ttgaaatatc cagatgggga acctgtggaa tgtgtgattt ctccatctac cattggtcgt
    30121 gttccagagg ctgcagcttc ccatgagttg tttaaaaaat caaatgtttg cgcaacaatt
    30181 acagttacac catgctggtg ttatggtagt gaaactatgg atatgtctcc agatattcct
    30241 catgctattt ggggatttaa tgggacagaa cgcccaggag ctgtctatct tgcagctgta
    30301 ctagcttcac atgctcaaaa agggattcca gcctttggga tttatggtag agatgttcag
    30361 gaagctaatg atacagatat tccagaagat gtcaaagaaa aacttttacg ttatgcgcgt
    30421 gcagctcttg caactggctt gatgagagac actgcttacc tatcaatggg tagtgtttcg
    30481 atggggattg gtggttctat tgtaaatccg gatttcttcc aagaatactt aggaatgcga
    30541 aatgaatcgg tagatatgac ggagttcaca cgccgtatgg accgtggtat ttacgaccct
    30601 gaagagtttg aacgtgctat ggtttgggta aaagaacata tcaaagaagg agttgaccga
    30661 aatcgtgagg atttgattct ttctaaagaa gagaaagaaa aacaatggga atttgttatt
    30721 aagatgttca tgattggacg tgacttaatg gttggtaacc caagacttgc tgaacttggt
    30781 tttgaggaag aagcagttgg tcaccatgct ttagtagctg gtttccaagg tcaacgtcag
    30841 tggacagacc attttccaaa tggagatttt atggaaactt tcctcaatac tcagtttgac
    30901 tggaatggta ttcgaaaacc atttgtattt gcgacagaga atgattcact aaatggtgtg
    30961 tctatgctct ttaattatct actaacaaat actccacaaa tctttgctga tgtgcgtact
    31021 tattggagtc cagaggctgt tgaacgtgta acaggatata ctttagaggg tcgtgctgca
    31081 gctggcttct tacatctaat caactctggt tcttgtacat tggatggtac aggtcaagct
    31141 actcgagatg gcaaacctgt tatgaaacca ttctgggagt tggatgaaag tgaagtacag
    31201 gctatgcttg aaaatacaga cttcccacca gcaaaccgcg agtacttccg tggaggagga
    31261 ttctcaactc gtttcttgac gaagggggat atgccagtaa caatggtacg tctcaatctt
    31321 ctaaaagggg ttggtccagt gctacaaatt gcagaaggtt acacacttga acttcctgaa
    31381 gatgttcacc atactttaga taatcgtaca gattcaggat ggccaactac ttggtttgct
    31441 ccacgtttga caggaaaagg tgctttcaag tctgtctatg acgtcatgaa taattgggga
    31501 gctaatcacg gagccataac atatggacac attggagcag acttgattac cttggcttct
    31561 atgttgagaa ttcctgtcaa tatgcataat gtacctgagg aagatatctt tagacctaaa
    31621 aattggtcct tatttggaac agaagatcta gaatcagcag actatcgtgc atgtcagttg
    31681 ttgggaccac tacataaata aaacttgttt atataggagg tgaacttacg tccctcctat
    31741 ccttttaaaa agatttgtta aacaattcac aaataattga aaacgaatac aaaaagtaat
    31801 ataatgatgt taaatagata gcgcggaggc gcaggaggaa aattatatgg ctatatttta
    31861 tgttccggca gtcaacctta ttggaaaagg tgttgtaaat gaagtgggtc cttatatcaa
    31921 ggaacttggc tataaaaagg cacttttggt gacagataag tacatcgaag gcagtgatat
    31981 tttacctaag actttaaaac cactggatac agaaggaatc gaatatgtca tctttagcga
    32041 tgtagagcca aaccctactt gtaaaaatgt cacagatggg gtagctgctt tgcaagaaca
    32101 tggctgtgac tttatcatca gtcttggcgg gggttctcca caggatgcag ctagttgtat
    32161 ttctatcatg gctacaaatg gtggaaaacc acaggattat gaagggcttc ataagtctgc
    32221 taaaaaaggt ttgccagttg tggctatcaa tacaacggca ggtacatcag cagaaattac
    32281 cattaactat gtgattactg atgaagaacg caaggttaag atggtaatgg ttgacaagaa
    32341 tagccttgcc ttgatttctg tcaatgaccc tgaactcatg ctttccaaac ctaaaggcct
    32401 gactgctgct actggtatgg atgctctgac tcatgctgtt gaagctttgg taacacctgg
    32461 tgcttatgat gtaaccaaga aactgtctat tggtgctatt gagcttatca aggaatatct
    32521 tcctcgtgct gtagaaaatg gacatgatat tgaagcgcgt gaaggtatgg tcaatgccat
    32581 cttccttggt ggtatgagct ttaataatgc tggtcttggc tatgttcact caatggctca
    32641 ccaactcggt gcagtatata atttgccaca tggcgtgtgc tgtgccatgt tgctaccagt
    32701 tatagaacgt gaaaatgcta aacgtgtacc agaagctttc cgcaatgttg ccaaagcctt
    32761 gggacttcat gtagaaggta aatcagatca agaatgtgcc gattatgcga ttgctgagat
    32821 tgagaagctt tctgagacag taggtattcc taagaaactg actgaacttg gtattgaaga
    32881 aaaagatttc gactttgaat acctttctaa gaatgccttg attgatgcct gcgcaccagg
    32941 aaatccattt atgccaacct tagaagaaac gattgccttt tataaagagt tattttagca
    33001 agagattgga cgacaatagg acaattcacc tctaattatt gacatttcag gaaaaatcgc
    33061 agtaaaaacg gcacagcctt ctttaaatgt gctataatac tagaaaaata cttgtggagg
    33121 ttccattatg gcaatatttt tcatgatttt tctgattgtt tgtgtgctcc tattggtgat
    33181 agtcacactg agtacagttt atgtggttcg tcagcagtcg gtggcgatta ttgaacgctt
    33241 tgggaaatac caaaaggttg ctaatagcgg tattcatatt cgcttgcctt ttgggattga
    33301 ctcgattgca gcacggattc agttgcgctt gttgcaaagt gatattgtgg ttgagactaa
    33361 gaccaaggac aatgtgttcg ttatgatgaa tgtagcgact cagtaccgtg tcaacgagca
    33421 gagcgtgaca gatgcttact ataaactcat acgtccagaa tctcagatta aatcttatat
    33481 cgaagatgct cttcgctctt ctgttccaaa attaaccttg gatgaattgt ttgagaaaaa
    33541 agatgagatt gcccttgagg ttcaacacca agtagcagaa gaaatgacca cttacggcta
    33601 cattatcgtg aaaaccttga ttaccaaggt cgaaccagat gcagaagtta agcaatctat
    33661 gaatgaaatc aatgcggcgc aacgtaagcg ggtcgcagca caagaattgg cggaagctga
    33721 caagattaaa attgtcactg cagctgaagc cgaagcagaa aaagaccgcc ttcatggtgt
    33781 ggggattgcc caacaacgta aggcgattgt ggatggattg gcagagtcta tcaccgaact
    33841 caaggaagcc aatgttggca tgacagaaga acaaatcatg tctatcctct tgaccaacca
    33901 gtatttggat accttgaata cctttgcctc taaaggaaat caaaccatct ttttaccaaa
    33961 tactccaaat ggtgtggatg atatccgtac acaaatcttg tcagcccttc gcgctgagaa
    34021 gaaataatag actaatactc ttcgaaaatc tcttcaaact acgtcagcgt cgtcttgccg
    34081 tatagatgtt actgacttcg tcagttctat ctgcaacctc aaaacagtgt tttgagctga
    34141 cttcgtcagt cttatctact acctcaaaac ggtgttttga gcaacctgta gctagtttcc
    34201 tagtttgctc tttgattttc attgagtata aagagcttgg caacaggctc tttttgtatt
    34261 gcgtttatta agaaaaacaa aaaacatcga tatacattta actaaaaatt attgtatgtt
    34321 atctccctta attaggaatg ataagggaat aactagaaag atttgtgaat acaaactatt
    34381 tctgatatac aaaatataca gtaataatga atgatgggag atgggatgaa agaatttcaa
    34441 tttgagagaa agcagcgttt ttctttgagg aaatatgcaa taggagcttg ttcggtcttg
    34501 ctaggaacga gtttattttt tgctggtatg ggtgctcagc ctgtacagga tacagaaacg
    34561 agttcagcac taatttcaag tcattatttg gatgagcagg atttatctga aaagctgaaa
    34621 tctgagttgc aatggtttga attagaaaac aagcttttga acttatggga gcattaggtt
    34681 actatgaagg atttgttcct tatgtttcaa atcaatacca aaaccaagct gaagaagaat
    34741 acaaaccgct atctgataaa tatattttcg aaaaaatctt aggaaaaaca tatgcagcct
    34801 tcaaaaaaga tcaaattaat gagcgtgtcg agaaattagg taagttaaaa cctattacaa
    34861 taaattacaa cggaaaatca gaagtaattg atagtaaaga aaaattacaa gagcttatga
    34921 ataaagccgt taaagacgaa gtggctcaaa tatagtaaaa tgaaataaga acaggacaaa
    34981 tcgatcagga cagtcaaatc gatttctaaa aatgttttag aagtagaggt gtactattct
    35041 agtttcaata tactataaaa tcaggcaata ccacggctaa aaagtttgag tttatcgaaa
    35101 taccagtgca aaaactgaaa aaagcaattt ataaagctca tctcaaagat tcagatgact
    35161 ttagaccaga aacatccaca ccaaatcttt ttgaaagctg tttgaagctt tgtccttgct
    35221 ttcttagttc atagatctga actttatctt cataactcaa tttcataata aaacacccca
    35281 aaagttagat ttattctgtc taacttttgg ggtgcagttc attaatcggt cgattttttt
    35341 tgtagaaaca gttgcctgat taagttgcaa gtcgatagac ggcttcggca taaatatcca
    35401 tagcacggta caaatcttct agaacggcac attcatttgc ctgatgttct gtctgcttcg
    35461 ctcctgggaa taaggcgccg aaggctacac aatttggcat ggtgcgagca aaagtggcac
    35521 caccggatga aatagcagga ctgttatcgc cagtcttttc ttggtagatt tgcatcagtg
    35581 tgctaacgag tttactgtct tttgcgacgt atagaggcgc tagatagtca aactcttcgt
    35641 agcggagttg gtagttttgt gcacatcttg taagcaactc tactagttct tccttgtcag
    35701 ctaagacagg agtccgaatg tcaatacgga tttcagaacg ttcatgattg atcatgagac
    35761 ctgcgacatt aaaggatagg tgaccagaag gttcatctgc tatatcacca aagatttgtc
    35821 ttcctgtgcc gtcttgacct gcttgtgttg caagaaaact gagagcaggg tgttcttgga
    35881 gaggagcaag aatggtagct agtcggatga cagcattgat accttgacta gcatccttag
    35941 catgctttgg cactccgaga accgttacgg tttgttcagt ggtttggtaa tcataaccag
    36001 cttctttgag accgttacaa acctgttcat agaggagacc ttggtagttg gccttgtctg
    36061 gtacaacgtt aaaggcgcct cctacttcaa gctctagttg atccgatccc gggccatgaa
    36121 gtttgacctg tagaagccct ttttcagcat aggtcagagg aaaagatgag tcaggtgcaa
    36181 agcccatact ggcctgttct tcgatggtat tgtagcgtgc catgcagcgc cagagggttt
    36241 cctcatcggt accaaagata aagcgtacgc gctttttgaa ctgaatacct tggtccagca
    36301 agctttttac tgcatagaga gctgcgagcg aagggccttt atcatcttgg acaccacgtc
    36361 cgaataccca gccgtctttg atagttgctt caaatggcgg tgtctgccaa tctgcttcat
    36421 cacctgatgg aacaacatcc aaatgacaga gaatggccag aagctctgct ccctgaccga
    36481 tttctgcata tccgtaataa cctttagggt caagataggt agtgaaacct atgtctcgac
    36541 aaatctctaa agttttttct aggacatctt ggattgcttg tccaaaaggt gttccatttt
    36601 ctccttcatt gagtactgaa ggataggaaa tcaaggtttt taatgatata agaaattcat
    36661 ctttaacttg atttgttata tctattttca tggaatcacc tcactcacta ttttatagga
    36721 atggaaggaa ggttccaagg agaagaaggg cgatggtcac tacaataata gcgactacga
    36781 gtttgcccat gaatttccac caagtaccaa tgttgatacg tccaagtgca agagctccca
    36841 tcacaatacc agatgttggt gcaatcaagt tcaagacacc tgaagcagat tggtaagcag
    36901 tgataatcaa gctaggacgg acatttacaa attctccaag tggagccatg atacccatag
    36961 ttgcgctggc aagaccagat gaagatggga tcaagaatga cataggtaga tagaagatat
    37021 aagttacaac gataaagact tgtgaagata gaccgctcaa gccttcttta ccccagttga
    37081 ggattgtatc ggtaatcata ccgtcgttca tgataacttg aataccacga gcaatcgcta
    37141 cgatcaaggc aacactgagc aagtcagcag caccattcat gaaggaagag ataatcttat
    37201 cttctttaag accataaata acaccaatca ggatacccat aaaggcaaag agcatggcgc
    37261 cttctgggaa gtaccaagta cctagtgcag aagtagatga accgacaata ttaccaataa
    37321 ctggaagacc agtcaaccaa gcattaaagt catcaaaaat ggtaacgcca aggtctgtcc
    37381 atggaatgaa gctcaatacc atcaagatga atgtcaacac aaataagaag agaactgatt
    37441 tttgtttgct gctaagtgta gattctacag atgaagattc ttctacgtta aagtgtttca
    37501 aatcttcttt gcgagtacta taaaccagtg acttagtcgg atctttttga atcttatccg
    37561 cataacggta aacaaaccaa gtactaagag cagtcaaggt aacccagaaa atcagacgaa
    37621 gtacgatacc gtcccctgta ccaactcccg cagtcgctga agcaatacct gtcgcaaatg
    37681 gattcagagt agatgccaaa cagccgattt gagaaccgag caaaataatt gcaacaccag
    37741 tcaggctatc aaaaccaacg gccatcataa ctggcacaag gagtggatag aaggccattg
    37801 tttcttcccc cataccataa gttgtaccac cgagggcaaa caaaggcatc agtaccaaaa
    37861 ttaacatttt ttcgcggccc ttatacttct tcacgataga ggcaatccct acgtcaagag
    37921 caccagtttt gttgacaatg ccaaggaaac caccaaccat aaggatgaag aaggctacat
    37981 caatcgctgc gctcgtttct ttaatgagcg aaccttcctc tggatgagta cctagcatag
    38041 cccgaatcgg tgccatgagg acatcccaaa tcccttgtgg attttgaggc tgagcctcgt
    38101 aaataccttc tataaaggcc cccgcaggga taaaccaagt tagcactgcc ataatagcaa
    38161 tgattatcaa taatacggtg taagatgaag gcatcttaaa ccctttttta gctttttcac
    38221 tcatttgttt ttcctcctga tgtctaacaa agtgtaaaaa accaactact ttcttaaata
    38281 aacatttaag aaaacgcttt ctatttatat ttgttttacc atattaaagt ctaaaaaact
    38341 aatatctttt gaaatattgt acatgttttc aagaataatt tctaatttga tataaagata
    38401 ctagaaatag aatacatctt attagtaaaa caacttatcc tttttcaata attgttccgc
    38461 tttcagattc aatcaaggcg cctagatttt caagggaagt aataactgct tttccttctg
    38521 gacgaccatt gacaaaagcg atagctgctt ctacttttgg aagcatgcta cctggtgcaa
    38581 actgatcttg tttgatatat tcttccagct gggcaacatt cacatgttcc aatttttcct
    38641 ggtttggctt gttgtagtta acaaatacat aatctacacc tgtcaaaacg atgaagaggt
    38701 ctgcatcaac caattctgcc aaacgttggg aagcgaagtc tttatcaata accgcttcga
    38761 caccagtcaa atgtccattg ttttctttga cgacgggaat accgccacca cctgcagcta
    38821 cgacgacttg accattattt aaaagagtac ggatggtttc aatttctttg atgtcaacag
    38881 gttttggtga ggcaacgacc ttacgccagc cacggccagc atcttccttg aaagtcgctc
    38941 cgctcttttc ggcttctgct tttgcttctt cttctgaata gaaaggaccg attggtttac
    39001 tcaagttaac aaaagctgga tcatttttat ctacgacaac ttgcgttaca acagaggcaa
    39061 catttttttc gatgccttca tccaagagag cattttgcaa agcatttttc aaccagaaac
    39121 cgatgctacc ttctgtcata gcgacaagtg agtcgagtgg gaaggcaggg ttcttttcag
    39181 agtctgatgc caaatgttgg agcaagagat tcccaacttg aggtccatta ccgtgagtga
    39241 taatcagatc atctccattt ttaatcaatt ttacaagatg cttagctgtt tcaactaaag
    39301 cttcttgttg agcctttgct gatgggtcag aagaaagaat cgcatttcct cccaaagcta
    39361 ctacaatttt acgatttgcc ataaattctc ctttatcaca ctcaatagaa tgcgtttaga
    39421 tttcaattta atgatttttc acatatttta taagaaataa tagattacca ttatataaaa
    39481 gaggaccgga ctaaagctat tagtcgcagc cctcatagct gttggtagac ggtttattat
    39541 ctaaaattat actttaggaa tataaaggtt accaagtgta gcagccataa cagctttgat
    39601 agtgtgcata cggttttctg cttgatcgaa gtggcgagcg tacttgctgc ggaagacttc
    39661 gtctgttact tccatttctt ctacaccaaa tttttcagca acgtctttac cataaacagt
    39721 gtgagtatcg tggaatgctg gcaagcagtg taggaagatc aagttttcat tgcctgcttt
    39781 tttaactaag tccatattga cttggtaagg tttaagaaga gctacacgtt ctgcgaattt
    39841 gtcttcttca cccattgata cccaaacgtc tgtgtaaaga acgtctgcat ctttaactgc
    39901 ttcatcagca tcttcagtga tgagaacatg tgcgccactt tctttagcaa atccttctgc
    39961 caattcaacg atttcttttt ctgggaagag ttcttttggt gagaagatgt gaacattgac
    40021 accaaggata gcacctgtta cgagcaagct gttggcaaca ttgttacgtc catcaccaca
    40081 gtataccaat gtcaagcctt ccaagcgacc gaagttttct tgaacagtca agtagtcagc
    40141 gagcatttga gttgggtgcc attcgtcagt tagaccgttc catactggaa cgcctgagaa
    40201 ttctgccaat tcttcaacca tacgttggct gaatccgcgg aattcaatcc cgtcaaacat
    40261 acgtcccaat actttagcag tatcttcagt agattctttt ttacccaact gaatatcatt
    40321 tgctccgagg tattctgggt gagcaccaag gtcgatagcc gcagttgtaa aggctgcacg
    40381 agtacgagta gatgtttttt caaataggag agcgatattc ttgccagcaa ggtagtggtg
    40441 ttgaatattg cgttttttca aatctttcaa gtgagctgaa agaccaataa ggtattctaa
    40501 ctctgcacgg gtaaagtctt tttctgctaa gaagctgcgt ccttggaata ctgaatttgt
    40561 cattttatta tttcctcttt ctatttttta cattttctat tgacgaatac cgaacagcga
    40621 ttacacttct tcacgttcaa atggcataga catacaacga ggtccaccac ggccccgaac
    40681 caattcactt ccgcgaatct taatcaagcg aagcccgtat tcttccaaaa tcttattggt
    40741 cacggtattg cggtcataaa caactaccac accaggtgcg atggtcaaag tgttagaacc
    40801 gtcgttccat tgttcacgcg cagctgctac gatattgcca ccaccgcaac gaatcaaatg
    40861 aactttttct acaccaaggt tttgagcaag aagttcagct aagtcacctt tctcttcaac
    40921 aattttaagt ttttcgtttt cgtaagtaac tgagtaaacg tgaaggtcgc cttcgatttc
    40981 tgggtgaata gtgaacttgt catagtctac catagtgaag acagtatcca agtgcatgaa
    41041 tttacggttg ttagcaaatt caaaggccaa aactttcttg aagccaacat ttttcttgaa
    41101 gatgttgacc aaaagttttt cgatagaagc tgcgtctgta cgttgagaga tacctactgc
    41161 aaggacgtct ttagaaagaa ctagctcgtc tccaccttcg atacgcgtat cttcttcacg
    41221 gttgtagacc aaatccactt tttcgccata gattgggtgg tatttgaaga tatacttacc
    41281 gtagagtgtt tcacggttac gagtgtctgc aaacatgtgg ttaagcgata cggcgtttcc
    41341 aattgttgca aatgggtcgc gagtgaaata gaggtttggc atcgggtcaa ttgcaaatgg
    41401 ataatctgat tcaactaagt cagttagatc tttagcttcg tcaggaattt ctggcaattc
    41461 aactttttga atcccagcca ttgttttttc aaccaattct tggttgtcct tgatgccgtg
    41521 aagcaattca cgaatagcaa ccttagtttg acgatcacgg atgttggctt cgtctaagta
    41581 ttcctcgata aattgatcgc ggatttctgg agaggtcaat gattcagcag cgagttgttc
    41641 taggtagaga acctcaattc cttcatcgcg aagagcttgg gcaaatgcat catgttcttt
    41701 ttgagcatct tccaagaaag gaatatcatc aaaaagaagc ctttcaagat agtccggcaa
    41761 caagttttct aactccttgc ctggacggtg caacataact tttttcagtt tcccaatttc
    41821 tgagaagacc tgaattggat gtgaagacat gtattttcct ccttaaaaat tttgacttgg
    41881 caccattttg ggtaccgctt acatgtatgt tataacgcaa ttcctaggta atgtcaatgg
    41941 ctataatttc tcggaaaata atataaatta tgatatttag cacaatttta tgttattata
    42001 cacaacatag gatggaatac atatatacaa atgcataaac ttctattaaa gaggatataa
    42061 aattttttta aaaaattttt caaaaaatac acaaataacc aaaaagtgtc caattatcat
    42121 ctggttatca cttggatctg tataaagaga aatgtttgtg tgaaaggata aaaagatgca
    42181 acattctttt gaagatagat tttggagaag catttcttgt tcaattgtac aaattataga
    42241 atcttttctg agtatttagg aagagataat tatcttattg accctctttt tattttttgt
    42301 tatagtcgac gaggtgatag tttagaataa tcacttattc acaatctgtg gaaaagtctg
    42361 gagagtgaat ggtcttcaca caatgacctg aaagaagcct gagaataatt atggagagta
    42421 gcattctgag aggtgttagc agaaccatat gacagagctg tttgaagagg gaatattgag
    42481 gagaaaaatc ctgagcctac cagttggagt tggaaagagc tggctgttag atcatggttt
    42541 attatccaca acctgtggat aaccttgtga acaagagaag ttgcaaaaga aggagatata
    42601 taacgatgaa gaaaatcaaa ccgcatggac cgttaccaag tcagactcag ctagcttatc
    42661 tgggagatga actagcagcc tttatccatt ttggacctaa tactttttat aatcaggaat
    42721 ggggaacagg tcaggaggat cctgagcgct ttaacccgag tcagttggat gcgcgtgagt
    42781 gggttcgtgt gctcaaggaa acgggcttca aaaagttgat tttggtggtc aagcaccacg
    42841 atggctttgt cctttatccg acagctcaca cagattattc ggttaaggtc agtccttgga
    42901 ggagaggaaa gggcgacttg ctccttgaag tatcccaagc tgccacagag tttgatatgg
    42961 atatgggggt ctacctgtca ccgtgggatg cccatagtcc cctctatcat gtggaccgag
    43021 aagcggacta caatgcctat tatctggctc agtttaagga aatcttatca aatcctaact
    43081 atgggaatgc tggtaagttc gctgaggttt ggatggatgg tgctagaggg gaaggggccc
    43141 agcaggtcaa ttatgaattt gaaaaatggt ttgaaaccat tcgtgacttg cagggcgatt
    43201 gtttgatttt ttcaacagaa ggcaccagta tccgctggat tggcaatgaa cgagggtatg
    43261 caggtgatcc actgtggcaa aaggtgaatc ctgataaact aggaacagaa gcagagctgg
    43321 actatcttca gcacggggat ccctcgggca cgattttttc aatcggagag gcagatgttt
    43381 ccatccgtcc aggctggttc taccatgagg atcaggatcc taagtctctc gaggagttgg
    43441 tcgaaatcta ctttcactca gtagggcgag gaactccact cttgcttaat attccgccga
    43501 atcaagctgg gctctttgat gcaaaggata ttgaacgact ttatgaattt gcgacctatc
    43561 gcaatgagct ctataaagaa gatttggctc tgggagctga ggtatctggt ccagctcttt
    43621 ccgcagactt tgcttgtcgc catttgacag acggccttga gaccagctct tgggcaaacg
    43681 atgcagactt gcccatccag ttagaactcg acttaggttc tcctaaaact tttgatgtaa
    43741 ttgagttaag agaagatttg aagctagggc aacgaatcgc tgcttttcat gtgcaagtag
    43801 aggtggatgg tgtctggcag gaatttggca ggggcttcac agttggtcat aagcgcctct
    43861 tgcgaggtcc gctagtagag gcacagaagg tgcgcgtgat gattaccgag gcacaatcta
    43921 ttcctgtatt gaccaagatt tccctttata aaactcctgg attatcaaaa aaagaagttg
    43981 ttcaggaact agcatttgca gaaaaaagcc tagctgtggc aaagggagaa aatgcctatt
    44041 ttacagttaa gcgcagagaa tgtagtggtc ctttagaagc taagatttcg attcaaccgg
    44101 ggacaggtgt ccatggtgtc gcctatcagg atgagattca agtccttgcg tttcaagctg
    44161 gtgagactga aaaaagtctg acgctaccaa ccttgtattt cgcaggagat aaaaccttgt
    44221 atttctatct gaacctaacg gtggatggtc agcttgtgga tcaacttcaa gtccaagttt
    44281 cataaaagaa gaacctttgc gcgatgcaaa ggttcttttg gttattagtg acttggtaac
    44341 cagctgaggg tgaaagttag ttgttcagct tttaagaggt cttggtgttg aatagttgat
    44401 acgagtattt tgtccagtcg gcattctttg acaaagttaa aatggttgtg gttttgttta
    44461 gtatggatat ccagccattt atcttcttta gctaggtaga ctcgtagatg gtcaaagaga
    44521 gggattccga ggtcatagct tggttttcct ggacaggttg gataaaatcc gagagctgac
    44581 cagatgtacc aagcagagag actaccattg tcttcatctc caggataggc ttcccaactt
    44641 gggtgaaaag ctttctgacg gagcgtcttg ataagaaggg cagtgtagtc agggtaatcg
    44701 ctgtagcgga agagataagg aatgtggaaa ctaggctggt tggaaatggc tatttgtcca
    44761 aaaggagcag tagccatctc gctcatttcg tgaatttcgt aaccatagcc tgttgtttca
    44821 aagaggggag catcttgaca ggctttcaaa agatagttgc taaaggtttc ttttccaccc
    44881 atcagttgga ttaagccagg gatgtcgtgg agaacgccta aagtagcttg aatggcagag
    44941 cattcagcgt agtctcgccc ccaactataa ggagagaagt cagggtgaaa gtttccttga
    45001 ttgtctcgtg ctcgcatgta acctgtctca gcgtcaaata gctggcggta attttgtgaa
    45061 gcagccttgt aggtttcagc gatttctatg ttctctagtt ttttggcaca gctggcgata
    45121 caaaagtcac tataggcata gtctagagta tggctaacac tttcatggtg gtcggtagag
    45181 aggtaaccta gttcttggta ttgggctagt ccgtggcggc cattgatgcc gagagggtcg
    45241 gctttgctgg ctgtttcgag catggcttgg aagagttctc cttctaggtc aggggccatg
    45301 tccttgcagg cgctatctgc gataataccg tctaaaagtg tacctggcat cataccccgt
    45361 tcatctggag ccagccattt tggaaggaaa ccagtatcgc ggtagctatt gaggaaacct
    45421 tctaaaaagc gttgatagtg ctccggtatg ataagggcaa agagggggaa ggtggtgcgg
    45481 aaggtatccc agaaaccatt gttgctaaag aggacaccag gcttgacagt accagtagcc
    45541 agatccatgt ggatggcttg ccctgactca tcaacctcat aaaaagtctg tgggaagagg
    45601 aagagtctgt agaggcagtg gtcaaagaag gttcggtcag cctctcctgt ctctataatg
    45661 tcaaaacgat ggaggagatt ttcccaatcc gcttgggcac ttgatttaca gctatcaaaa
    45721 tcttcttgag gtagattgat tagagcttga gaaggagaga tgaaagaagt ggctagttgc
    45781 atctcggttt gactacttac taagtcaatt cgccagtctc cagcttcttg gctgatagca
    45841 agaatatccg tgttcatttg cagggcagtg aacatcgtta gcgaattttt gttagtttca
    45901 gttttacctt cttgtcgcag ggcaagagtc cgcttatcta cttgctctac tgtcagttca
    45961 tctgctgcgt gaagatagag ggagagggct ttgccttgct tttgattcaa acgaatagaa
    46021 gcaccatagc aagttggtgt gagctgggtt tcaatctgat aacgcagaga aaagagcttc
    46081 aaatagtgag gctggaagca agctttatct atatcataag aagactggcg gtgaaagagg
    46141 ctgtctcccc ccagttgact ggtgacaggt gtcagaagga gccaagagta gtccccaatc
    46201 caaggactgg gctggtgagt taatcgaatc ccctgaaaga taggcagatg cggatcaaaa
    46261 aaccaagatc catcctggtc actggtctgg ggcacaaagt aattcatccc aaaaggcacg
    46321 cctgtgtatg gcagggtatt tccccaagag aaggcatgct tgctagcagt accaaagcgg
    46381 gtatcgatgg tttcaagtag tggtttcata gtctttcctt tagctgtttt tctacattat
    46441 atcagaaaaa gagggccttt agaaaaggag tttcaaaaat aactattttg tagaaaaatg
    46501 actatcattt gtgtatgtat tttctgtctc aaaagctata tactgaacat gaaacttgtt
    46561 tgaaagaggg aattgcacga tgatttatcc gaaagaaatt gttagagaat ggctagatga
    46621 agtagcagag cgggctaagg accatccaga gtgggtggat gttttcgagc gttgctacac
    46681 cgataccttg gacaatacgg ttgaaatctt agaagatggt tcaacttttg tcttgactgg
    46741 ggatattcct gctatgtggc ttcgggattc gacagcccaa ctcagaccct acctgcatgt
    46801 agctaaaaga gatgccctcc tgcgtcagac cattgcaggt ttggtcaaac gtcagatgac
    46861 cttggtactc aaggatcctt atgccaattc cttcaacata gaggaaaact ggaaggggca
    46921 ccacgagacc gaccacaccg accttaatgg ctggatttgg gaacgcaagt atgaggtgga
    46981 ctcgctttgc tatcctttgc agttggctta tctcctctgg aaagaaactg gcgagactag
    47041 tcagtttgat gagacttttg tcgcagcgac taaggaaatt ctccatctat ggacagttga
    47101 acaagaccac aagaactctc cttatcgttt tgtccgagat acggaccgta aggaagacac
    47161 cttggtaaat gatggctttg gacctgactt tgcagtgaca ggtatgactt ggtcagcctt
    47221 tcgtccgagt gatgactgtt gccagtatag ttacttgatt ccgtcaaata tgtttgctgt
    47281 agtagtcttg ggttatgtgc aagaaatctt cgcagcatta aacctagctg atagccagag
    47341 tgttattgct gatgccaagc gtcttcagga tgaaatccaa gaaggaatcg aaaactacgc
    47401 ttacaccacc aacagcaagg gcgaaaagat ttacgctttt gaagtagatg gcctaggaaa
    47461 tgccagcatc atggatgatc caaatgtacc aagtctacta gctgcgccct atctgggcta
    47521 ctgttcgatc gatgatgaag tgtatcaagc cactcgccgt accattctga gctctgaaaa
    47581 tccatacttc taccaaggag aatacgctag cggtctcgga agttctcata ccttctatcg
    47641 ctatatctgg ccaatcgccc tttctatcca aggattgaca acaagagata aggcagagaa
    47701 aaaattcttg ctggatcagc tggttgcctg cgatggtggt acaggtgtca tgcacgaaag
    47761 cttccacgta gatgatccga ccctctactc tcgtgaatgg ttctcctggg ctaacatgat
    47821 gttctgtgag ttggtcttgg attacttgga tattcgctaa ggggctcgct ttagctcaac
    47881 cgattcttat cagaatcaca agtttacatt taaaacgtta aaatttaaat ttagaatgag
    47941 gttttacttc atggaaaatg ttgttgtaca tattatctca catagtcact gggaccgtga
    48001 gtggtacttg ccttttgaaa gccatcgtat gcagttggtg gaattgtttg acaatctctt
    48061 tgatctcttt gaaaatgacc ctgagttcaa gagtttccac ttggatggac aaactattgt
    48121 ccttgatgac tacttacaaa ttcgccctga aaatcgcgac aaggtccaac gctacattga
    48181 cgagggcaaa cttaaaattg gtccctttta catcttgcag gatgactact tgatctccag
    48241 tgaagccaat gtccgcaata ccttgattgg tcaacaagaa gctgccaaat ggggtaaatc
    48301 aacccagatt ggctactttc cagatacctt tggaaatatg ggacaagcgc ctcaaattct
    48361 tcaaaaatca ggcattcacg tggcggcctt tggtcgtggt gtgaagccga ttggatttga
    48421 caaccaagtc cttgaagatg agcagtttac atcccagttt tcagaaatgt actggcaggg
    48481 tgtggatggt agtcgtgttt taggtattct ctttgccaac tggtacagta atgggaatga
    48541 aatcccagtt gacaaagatg aggccttgac cttctggaaa caaaaattgt cagatgtgcg
    48601 tgcctacgct tcgaccaacc aatggttgat gatgaacggc tgtgaccacc agcctgtaca
    48661 gaaaaatctg agcgaagcca ttcgtgtggc aaatgaactc ttcccggatg taatctttgt
    48721 tcatagttct tttgatgaat atgttcaagc tgtagaaggt gcgcttcctg aacacttatc
    48781 aactgttaca ggcgagttga ccagtcagga aacagatggc tggtacacac ttgccaacac
    48841 ttcttcatcc cgcatttacc taaaacaagc cttccaagaa aatagcaacc tcctagagca
    48901 agtggtagaa cccttgacta ttatcactgg tggacacaac cacaaggacc agttgaccta
    48961 tgcttggaaa acacttttgc agaatgcgcc acatgatagt atctgtggct gtagcgtgga
    49021 cgaagttcac cgcgagatgg aaacgcgttt tgccaaggtc aaccaagtag gaaactttgt
    49081 taaaagtaat ttgctcaacg agtggaaggg taaaattgct acggataagg ctcaaagtga
    49141 ctatctcttt actgtcatta acacaggctt gcatgataag gtcgatactg tcagcacagt
    49201 gattgatgtg gcgacttgtg atttcaagga attgcaccca acagaaggct acaaaaagat
    49261 ggctgctctt atcttgccaa gttaccgtgt ggaggacttg gatggtcgtc ctgtagaggt
    49321 tacaatcgaa gacctcggag ctaattttga atacgattta ccaaaagata agttccgcca
    49381 agctcgtatt gctcgacaag tgcgcgtgac cattccagtt cacctagcac cgctttcttg
    49441 gacaaccttc caattgctgg aaggagaaca agaacaccgt gagggtattt accaaaacgg
    49501 agtgattgat acaccattcg taacggtgag tgtggatgac aacatcacag tctatgataa
    49561 gacaactcac gaagcctatg aagattttat ccgcttcgaa gaccggggtg acatcggaaa
    49621 cgagtatatc tatttccaac caaaaggaac agagccaatc tttgcagagc ttaagggcca
    49681 cgaggtcttg gaaaacacag atcgctatgc taaaatcttg ctcaaacatg aattgaccgt
    49741 gcctgtcagt gcggatgaaa agctagaaga agagcaacaa ggtatcatcg agtttatgaa
    49801 gcgtgaggct ggacggtcag aagaattgac aaacattcct ctggaaactg agttgattgt
    49861 ctttgttgac aatccacaaa tccgcttcaa gactcgcttt actaacactg ccaaggatca
    49921 ccgtatccgt ctcttggtca agactcataa cacgcgtcca agcaatgatt ctgaaagtat
    49981 ctatgaggtg gtgacacgac caaacaaacc agctgcttca tgggaaaatc ctgaaaatcc
    50041 tcaacaccaa caagcttttg tcagtctgta tgacgatgaa aaaggggtga ctgtatccaa
    50101 caagggattg aatgaatacg aaatccttgg ggataacacc attgccgtga ccattttgcg
    50161 tgcatcaggt gagctaggtg actggggcta cttcccaacg ccagaagcac aatgcttgcg
    50221 tgagtttgaa gtcgaatttg cgattgaatg ccaccaagcc caagaacgct tctcagccta
    50281 tcgtcgtgcc aaagccttgc agacaccgtt taccagcctt cagcttgcta gacaggaagg
    50341 aagcgtggtt gcgactggta gcctcttgag ccattctgtt ctcagcatac cgcaagtttg
    50401 tccaacagcc tttaaagtgg cggaaaatga agaaggctat gtcctccgtt actataatat
    50461 gagtcaagaa aatgtgcgca tatcagaaca tcaacaaacc attcttgact tacttgagcg
    50521 accatatcca gttcattcag gactattggc tccacaagag attcgtacag aattcatcaa
    50581 aaaagaagaa atttaatttc aaaaagtaaa catcaaaaga aaggaggggc gaaaaagtaa
    50641 gaactaactg ctgattcgcc cctttttatg gtaaaaacaa tgaccattgc aacgattgat
    50701 atcggaggga ctgggattaa gtttgccagt ctgactcctg atgggaaaat actggataag
    50761 acaagtattt caacgcctga aaacttggag gatttactag cgtggctaga tcaacgcttg
    50821 tcagaacagg attacagtgg gattgctatg agcgttccag gtgcggtcaa tcaagagaca
    50881 ggtgtgattg atggcttcag tgcggtgccc tacatccatg gcttttcttg gtatgaggcg
    50941 cttagctctt atcagctacc tgtccattta gaaaatgatg ccaactgcgt tggactcagt
    51001 gaactactag ctcatccaga gcttgaaaat gcagcctgtg tcgtgattgg gacagggatt
    51061 ggcggagcca tgattatcaa tggtagactt tatcgaggtc gccacggtct gggtggagaa
    51121 tttggctaca tgacaaccct tgcccctgct gaaaaactta ataactggtc gcaactagca
    51181 tcaactggga atatggtacg atacgtgatt gaaaaatctg gtcatactga ttgggacggt
    51241 cgcaagattt accaagaggc cgcagctggt aatgctcttt gtcaagaagc cattgagcgc
    51301 atgaaccgca atctgacgca aggcttgctc aatatccagt atctgatcga tccagatgtc
    51361 atcagtctgg gtggctctat cagtcaaaat ccagatttta tccaaggtgt taagaaggct
    51421 gttgaagact ttgtcgatgc ctacgaagaa tacacggtcg caccagttat ccaggcctgc
    51481 acctatcacg cagatgccaa tctctacggt gcccttgtca actggctaca ggaggaaaag
    51541 caatggtaag atttacagga cttagtccca aacaaacgca agctattgag gttttaaaag
    51601 gtcacatttc tctaccagat gtggaagtgg ctgtcactca gtctgaccaa gcctctatct
    51661 ctatcgaggg tgaggaaggt cactatcaat tgacctaccg caaacctcac caactttatc
    51721 gtgccttatc cttgttggta acagttctag cagaagctga taaagtagag attgaggaac
    51781 aagcagctta cgaagatttg gcttacatgg ttgactgttc tcgaaatgcg gtgctgaatg
    51841 tggcttctgc caagcagatg attgaggtct tggctctcat gggctactca acctttgagc
    51901 tttacatgga agacacttac cagattgaag gacagcctta ctttggctat ttccgtggag
    51961 cttattcagc agaggagttg caggaaatcg aagcctatgc tcaacagttt gacatgacct
    52021 ttgtaccatg catccagacc ttggcccact tgtcggcctt tgtcaaatgg ggtgtcaagg
    52081 aagtgcagga gctccgtgat gtagaggaca ttcttctcat tggcgaagaa aaggtttatg
    52141 acctgattga tggcatgttt gccacgttgt ctaaactgaa gactcgcaag gtcaatatcg
    52201 ggatggacga agcccacttg gttggtttgg gacgctacct gattttgaac ggtgttgtgg
    52261 atcgtagtct cctcatgtgc caacacttgg agcgcgtgct ggatattgct gacaaatatg
    52321 gtttccactg ccagatgtgg agtgatatgt tcttcaaact catgtcagcg gatggccagt
    52381 acgatcgtga tgtggaaatt ccagaggaaa ctcgtgtcta cctagaccgt ctcaaagacc
    52441 gtgtgactct ggtttactgg gattattatc aggatagcga ggaaaaatac aaccgtaatt
    52501 tccgcaatca tcacaagatt agccatgacc ttgcatttgc agggggagct tggaagtgga
    52561 ttggctttac acctcacaac cattttagcc gtctagtggc tatcgaggct aataaagcct
    52621 gccgtgccaa tcagattaaa gaagtcatcg taatgggttg gggagacaat ggtggtgaaa
    52681 ctgcccagtt ctctatccta ccaagcttgc aaatctgggc agaactcagc tatcgcaatg
    52741 acctagatag tttgtctgcg catttcaaga ccaatactgg tctaacggtt gaggatttta
    52801 tgcagattga ccttgccaac ctcttaccag acctaccagg taatctcagt ggcatcaatc
    52861 ccaaccgcta tgtcttttat caggatgttc tctgtccgat acttgaccga cacatgacac
    52921 ctgaacagga caagccacac ttcgctcagg cggcgggaac tattgctgac attaaagaaa
    52981 aagctggaaa ctatgcttat ctctttgaaa ctcaggccca gttgaatgct attttaagta
    53041 gcaaagtaga tgtgggacga cgcattcgtc aggcctacca agcggatgat aaagaaagtt
    53101 tacaacaaat cgccagacaa gaattaccag aacttagaag ccaaattgaa gacttccatg
    53161 ccctctttag tcaccaatgg ttgaaagaaa acaaggtctt tggtttggat acagttgaca
    53221 tccgtatggg cggactcttg caacgcatca aacgagcaga aagccgtatc gaggtttatc
    53281 tggctggcca gcttgaccgc atcgacgagc tggaagttga aatcctacca tttactgact
    53341 tctacgcaga caaggatttc gcagcaacta cagctaacca gtggcatacc attgcgacag
    53401 cttcgacgat ttatacgact taatactctt cgaaaatctc ttcaaaccac gtcagcttcc
    53461 atctacaacc tcaaaacagt gttttgagca gcctgcagct agcttcctag tttgctcttt
    53521 gattttcatt gagtataaaa acaagaacac cttgcttggc gcagggtgtt tcgcgtgaaa
    53581 cagaagaatt atctggtttc aaatgctaca gttagacaaa cttatgataa aatagcagaa
    53641 agtgaatgtt tcctaagagc aattctatcg atgagcaatt ggaggtatta tgctacactt
    53701 aaaattagta aaacaagaaa tagaagctga aaagccagca tctgtagaag cttggatcat
    53761 ttccgtcaaa tttaaaaaag gttgctaccg acatatatag attccaaaaa caaaaacgtt
    53821 agcggaacta gcagatgtga ttttatgggg ttttgatttt gcaaatgatc atgctcacgc
    53881 atttttcatg gataatgttg agtggagtca tgcagattct tactttctta gctttgttag
    53941 tgacgatgtt gaagaacgtt acacagaaaa tgtctatctg gatagcctaa gtgtcaaaca
    54001 aaaatttaag tttattttcg acttcggtga tgaatggcgt tttgaatgcc aagtactgag
    54061 agaaatcgag acagaggacg aagaagctta tctcgtacgt tcggttggaa cgtcgccaga
    54121 acaatatcca gattatgatg gttttgacta tgaagaatgg taaaattgaa atctaaaaaa
    54181 caattggaat cagtctgtgt aggcttagta tttcaataga cttcctgcaa aactagaatc
    54241 ctaattcatg attgataata ccagcactca aattcattcg taatccgaag cgtttacgat
    54301 gatttcgata ggttgttgaa aacattttaa acgtttttac tttggcaaag atgttctcaa
    54361 ccttgcttct ctccttagat agcgcatggt tacaggcttt atcttcaact gttagcggct
    54421 tgagtttgct ggatttacgt ggagtttgtg cttgaggata tatcttcatg agcccttgat
    54481 aatcactgtc agccaagatt ttaccagaat gattcgctaa ttgttttttt agggcgattg
    54541 atttgtactt ccgtcgcatc aatcattacc gtgtcctcag aactgagagg agttcttgaa
    54601 atcgtaacac cactttgaac aagagttact tcaacccatt ggctccgacg gattaagttg
    54661 ctttcgtgaa taccaaaatc agccgcaatt tcttcataag tgcggtattc tcgcacatat
    54721 tgaagagtgg ccataagaag gtcttctagg cttaatttag gttttcgtcc accttttgcg
    54781 tgtttaagtt gataatctgt ttttaacaca gctaacatct ctttaaaagt ggtacgctga
    54841 acaccaacaa gacgcttaaa tcgtgtatca gttaattgtt tacttgcttc ataatttcgc
    54901 agggagtcta ttgactcttt ggtaggtgtc aatgtttttt tcatctatac cgagaattat
    54961 tttcccgcca tttgtatttg caaatgctga gtaggtttcc cagaaagact ctggaagatt
    55021 gtttttagct tttttgtatt ctaaatcaac cccttcaaat tttaagtcca tatttttcct
    55081 ttacatctgt tttttgtggt tctggtattt gttcaagttg agtgataata tagcgaattg
    55141 aatttcgaga gtttttaacc cgctttaatt tcttttttaa cccgctttaa tttctttttt
    55201 aacacgtgt
//
LOCUS       DAVKFH010000008        54756 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKFH010000008 DAVKFH010000000
VERSION     DAVKFH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.008"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..74
                     /locus_tag="VW006_000473"
     tRNA            1..74
                     /locus_tag="VW006_000473"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asn,seq:gtt)
     gene            182..1028
                     /locus_tag="VW006_000474"
                     /pseudo
     CDS             182..1028
                     /locus_tag="VW006_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1063..1860)
                     /locus_tag="VW006_000475"
                     /pseudo
     CDS             complement(1063..1860)
                     /locus_tag="VW006_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2126..2362
                     /locus_tag="VW006_000476"
     CDS             2126..2362
                     /locus_tag="VW006_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9379384.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            2593..3879
                     /locus_tag="VW006_000477"
     CDS             2593..3879
                     /locus_tag="VW006_000477"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9379385.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(4143..5291)
                     /locus_tag="VW006_000478"
     CDS             complement(4143..5291)
                     /locus_tag="VW006_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9379386.1"
                     /translation="MKYNKTKYPNIFYYETAKGKRYYVRRSFFFRGKKREKSKSGLTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSTLLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(5470..6393)
                     /locus_tag="VW006_000479"
     CDS             complement(5470..6393)
                     /locus_tag="VW006_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEU9379387.1"
                     /translation="MSYSYVALDVETANDFRGSVCSIGLVKFKDGNIVDAFYTLINPE
                     EEFDDFNIFIHGITPEDVLDSPTFPEVRKAIVDFIGSDIVVAHFAQFDMGALKDVYQK
                     YELDFDNIEYICSYRLAKVALPGQLNYKLKRLAKNLNIELDHHNALSDARASGLILEY
                     LLSTNSFSDLNAFLKEYSYNKTGLLGQYGFKRKKSYQYKENLIYQPTEEEKAAMNPDH
                     YFYGLYFCFTGKLERMTRKEANKATALVGGIPEKGVTKHTNILVVGEQDWRVVGTDGL
                     SSKMKKAQTLLEKGQDIEIMTENDFIRLLEE"
     gene            complement(6406..6789)
                     /locus_tag="VW006_000480"
     CDS             complement(6406..6789)
                     /locus_tag="VW006_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU9379388.1"
                     /translation="MTEKEIISHFQIRIIDFDGDLMPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDAIESLDDPTEFDYLK
                     FMSYYNLKTMTNEVMVKEEYFALME"
     gene            complement(6802..7164)
                     /locus_tag="VW006_000481"
     CDS             complement(6802..7164)
                     /locus_tag="VW006_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379389.1"
                     /translation="MFFTFEKIKELADKQGISLNKLEEKLGFSRNTIYNMKKSTPNVE
                     RVSMIADYFNVSTDYLLGRTDNPTIAGSHDYKWEGKTLNVEEMASNVMMFGGRELTDE
                     KKKIIQSIIEGYLKEAGD"
     gene            complement(7542..7763)
                     /locus_tag="VW006_000482"
     CDS             complement(7542..7763)
                     /locus_tag="VW006_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379390.1"
                     /translation="MSKLTKEDVLQVSQEIINDAIPVIQDMLDEVFKEYPIDMEIRKA
                     ILNSVLVAHKLSTETTVSLLTELVNAQEN"
     gene            7882..8028
                     /locus_tag="VW006_000483"
     CDS             7882..8028
                     /locus_tag="VW006_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379391.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLFKQASHEIELRALES
                     ETKK"
     gene            8040..8243
                     /locus_tag="VW006_000484"
     CDS             8040..8243
                     /locus_tag="VW006_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379392.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVASSVISELLKYG
                     KGSESVKEKVADVLGIENPWRNH"
     gene            8386..9099
                     /locus_tag="VW006_000485"
     CDS             8386..9099
                     /locus_tag="VW006_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEU9379393.1"
                     /translation="MNEIFNFHGQEVRTLTIDDEPWFVGKDVADILGYSKARNAIALH
                     VDEDDALKQGLTDNLGRVQETIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRK
                     QGGFIREDLDEDAFIALFTGQKKLREQQVTMLEDIDYLKSEQPIHPSYAQSLLKKRKA
                     RVVACLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNISRYDLLPKKFADAALAYWMTW
                     EPSTNTKMKIMKLNSFDDV"
     gene            9113..9370
                     /locus_tag="VW006_000486"
     CDS             9113..9370
                     /locus_tag="VW006_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9379394.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYSELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            9456..9776
                     /locus_tag="VW006_000487"
     CDS             9456..9776
                     /locus_tag="VW006_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379395.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQGYNHAYERGREAERQEIRKNIRRPFKGFTYDNEPPQGLRPEPLALPEPKQSA
                     IRFL"
     gene            9792..10085
                     /locus_tag="VW006_000488"
     CDS             9792..10085
                     /locus_tag="VW006_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379396.1"
                     /translation="MEELIEWLETKEKLNREMISDEETSDFNLHLENDNDTMFMIKEY
                     LNDYNKLAKDYRILSSENRLLKLEKMELEGRHIYEDMRMKYRANRRKWGARYV"
     gene            10078..10884
                     /locus_tag="VW006_000489"
     CDS             10078..10884
                     /locus_tag="VW006_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU9379397.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNKNRLDK
                     DKNKKRITTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENDVDLVKEALKTAI
                     NSGKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            11024..11794
                     /locus_tag="VW006_000490"
     CDS             11024..11794
                     /locus_tag="VW006_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9379398.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LAFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            12003..12677
                     /locus_tag="VW006_000491"
     CDS             12003..12677
                     /locus_tag="VW006_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9379399.1"
                     /translation="MMKFLNGDCMDIMKQYPDNYFDLAIVDPPYFSGPEKRKFYGRKI
                     SPIGVSRLYGEISEWKIPNRDYFDELFRVSKNQIIWGVNYFDYSFGSGRIVWDKVNGQ
                     SSFSDCEIAYCSYHDSTRLFRYMWNGMMQGKSISEGHIQQGNKALNEVRIHPTQKPVN
                     LYLWLLQTYAKEGDKILDTHVGSASSLIACGELGFDYVGCEIDKSIFNLAQQRLDAYE
                     KQLKLF"
     gene            12687..12914
                     /locus_tag="VW006_000492"
     CDS             12687..12914
                     /locus_tag="VW006_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379400.1"
                     /translation="MLELYFVYNGHCKFYLGRFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            13041..13208
                     /locus_tag="VW006_000493"
     CDS             13041..13208
                     /locus_tag="VW006_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379401.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            13195..13404
                     /locus_tag="VW006_000494"
     CDS             13195..13404
                     /locus_tag="VW006_000494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379402.1"
                     /translation="MKYDKQAEIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LKKKNKELRRKAEELEDDGRVKEKS"
     gene            13376..13693
                     /locus_tag="VW006_000495"
     CDS             13376..13693
                     /locus_tag="VW006_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379403.1"
                     /translation="MMEELKKKVNAVYNWTVEDGKPQPPQQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     GGN"
     gene            13695..14204
                     /locus_tag="VW006_000496"
     CDS             13695..14204
                     /locus_tag="VW006_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9379404.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPEWFYENNIEKFCLAWLDGYEVEKE
                     KRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDANFGWVFDCEG
                     IDIEEVENE"
     gene            14197..14787
                     /locus_tag="VW006_000497"
     CDS             14197..14787
                     /locus_tag="VW006_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9379405.1"
                     /translation="MNKDLIETPRFNFFIGDEVLLKGKIVGFDVDENKCVENVVRLEY
                     GQTLNVPNNNIYITDDIVDKSKIKVVVPQFVADWYEENKDSFEFNVCDWIAFRDEAKK
                     SENREFNNWINNSRENPIQTLVNMNQFGYEVEEEKRYLVTLKNRQPLVKSQSGSTLYF
                     SQDITARNYKGTQKELEDANFGWVFDCPGIEIEEVE"
     gene            14945..15205
                     /locus_tag="VW006_000498"
     CDS             14945..15205
                     /locus_tag="VW006_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEU9379406.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYEKVKVGDEVML"
     gene            15202..16284
                     /locus_tag="VW006_000499"
     CDS             15202..16284
                     /locus_tag="VW006_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9379407.1"
                     /translation="MKFLDLFAGIGGFRLGMESAGHKCIGFCEIDKFARESYKAIHNT
                     KGEIELHDITAVSDESIRGIGSVDIICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYNVEWQVLNSKNFGVPQNRER
                     VFIIGHLRRERTRRIFPLSGKNQSTSNQSVMKIGNINPSGNGMNGEVYQADGLAPTLT
                     TNKGEGQKIAIKSNTIKQFGVLQPNFNQCGVVYETDGIAPTIRAYQGGGLEPKIIQRG
                     HGYNQGGEHDIAPTLTSNRYHENNHLSDGFRIRKLTPKECWRLQGFPDWAFDKAQEVN
                     SNSQLYKQAGNSVTVNVIAAIAKELS"
     gene            16320..16388
                     /locus_tag="VW006_000500"
                     /pseudo
     CDS             16320..16388
                     /locus_tag="VW006_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            16385..16783
                     /locus_tag="VW006_000501"
     CDS             16385..16783
                     /locus_tag="VW006_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379408.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIISEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTYIGGMP
                     AVYLKGFGDGYAWTEYEEIIGNIYENPVRR"
     gene            16787..17212
                     /locus_tag="VW006_000502"
     CDS             16787..17212
                     /locus_tag="VW006_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9379409.1"
                     /translation="MAIDIKKRLKALPYIDIKAKSKHQEIISLKSGILRGQQFDSMPK
                     SKSNKNQTEELNVLIIDKSEQLYKEIKQMYHERDELVQAIESLDDPVENIVMRLLYID
                     GLSWKEVQIKLNCSPATIQRAKHKALLKLSKMYDKNDSK"
     gene            17299..17865
                     /locus_tag="VW006_000503"
     CDS             17299..17865
                     /locus_tag="VW006_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9379410.1"
                     /translation="MVVVEPIRNRDDVQLMIEWLALHSAVKESDRQRNLMLFLSGVNL
                     GFRIGDIVKLKVKHVKGWHVQIVDEKTDKPTKRKMPKKFKNAMRQYIKDKKDEDFLFP
                     SRNGKHQHIKPNTAYKIIKRAAEEVGLENIATHSMRKTFGLFMYEQTKDVALIMDLLN
                     HSSQSISLRYIGKNQDSQDRAMTKFQGF"
     gene            18031..18237
                     /locus_tag="VW006_000504"
     CDS             18031..18237
                     /locus_tag="VW006_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379411.1"
                     /translation="MKGIIKRLFNKRTTKQKPLGKIVVGVEIENRSELKELTQQCCEA
                     IEHLNNCIDKLNKFELKVSTSIIK"
     gene            18234..18530
                     /locus_tag="VW006_000505"
     CDS             18234..18530
                     /locus_tag="VW006_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU9379412.1"
                     /translation="MIEVSTREERNQFYNSSEWRTIRRQALKRDHYECVWCRDEGKVT
                     TTNLEVDHIKELELYPEFALDIDNLRTLCKACHNKRHDRFDKNDRNFRKDEWWG"
     gene            18649..18987
                     /locus_tag="VW006_000506"
     CDS             18649..18987
                     /locus_tag="VW006_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379413.1"
                     /translation="MEEYSEKNIKELENQLLSKIGYFSPRKKDAIQYEKVNRYLYLVR
                     LLYELKAKLHEDGLVITVHNGQQRFQKANSLIKEINTTSNQLLAIERSFDFEVENSPV
                     EKPTSGSDLL"
     gene            18984..20624
                     /locus_tag="VW006_000507"
     CDS             18984..20624
                     /locus_tag="VW006_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEU9379414.1"
                     /translation="MISHPLVDDYIKMAESGEIVVNKERKLLFKIIKEKIYPRDDLYF
                     DNDLIDKFIRFAEKNFFPLAKYQLFLTPFIFLFRKEDGEPHFDEYLYTLARGGGKNGF
                     MSARSSFFISPIYPIRDYDVTITANSEKQGKVSFEEVYETIQRRGLEDHFYLTKMSIT
                     GRANNSVFSFRTNNPKTMDSARDGCLEFDEIHQFEDDKAVKVQRSGLGKIAHARTFYN
                     GTNGYVREGFYDKLIEKSMQILNGEVDDFRLFPFICKLDSADEVDDMKKWPKANPMLD
                     ESTPYAKRLLARTKADYDDLELEPSGRQEFMTKRMNLPEADLEKDVTSREKLVACLRS
                     PGIDLLGKSCVAGFDYASIRDFASVGLLFKNGDEFIWKQHSFARKVFLKAFKLKAPIQ
                     EWADKGLFTIVDGPSIDPRLLVAKLEEWSKFYQIELVCADGFRMDLLKPLLEEAGFEY
                     EFLRNPGAIQSKVAPIIEDGFANERFVFENDNSMIWYTDNTYIKEDKDGNKRFLKKEP
                     VRRKTDGFHALIAALYKRELVHESNVGEFLDMLDSWDF"
     gene            20681..21829
                     /locus_tag="VW006_000508"
     CDS             20681..21829
                     /locus_tag="VW006_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9379415.1"
                     /translation="MGFLNLFKRDAPDVGFDFEELERMLGRLQLKSLAIDKSAEFVAR
                     IFAKSEFRYMVENHHVTSDWDYILNVRPNKNESASEFWQKVIYRLLTKNEVLIVLSDD
                     KQLLVADAFTRRRYALYDDTFEMVSVGDYTFQRKFAMSDVIFLQYNNNRLQEYVSDLF
                     SDYEKLHTRLVEALARNNQIRGVLATKANGTFDEDKRKKLQRYADNLFKSFTSKTVAI
                     VPTMDGVEYSELTNTVGTTNLSVEELKKLRRQFDDEVADILGLPTALMHGDMADLSNS
                     QKMFNSYCYESLVKKVGDALNHAMVSSAGYANQKKFVIFGEGGLDKFALAENIDKLIS
                     SGAMTRNEVRLELGLEAVPGGDSFLITKNYQGENEIEKGGNEDDNHSD"
     gene            21810..22532
                     /locus_tag="VW006_000509"
     CDS             21810..22532
                     /locus_tag="VW006_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEU9379416.1"
                     /translation="MTIIQIKGAIVSNDDRWFYDWIDMDATAPKDIVLPTTGEDVEVH
                     INSGGGDVYAGSEIYTALRAYQGHVTVKIVGIAASAASVIAMAGDQVEISPTAQIMIH
                     NVSAGVRGDHKALLHEAGVLEGFNKSIAYAYIDKTGKALDDLLDLMEGETWFDARSAV
                     NHGFADKVMFESQEAPVLVASQTPVIPRDFIEKIRSAMTPDVELLADLVRDRLNNSLL
                     IPEDKKKEDKTAEPVGLGRFPF"
     gene            22551..23666
                     /locus_tag="VW006_000510"
     CDS             22551..23666
                     /locus_tag="VW006_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEU9379417.1"
                     /translation="MMKLGNEFTTARQNFMNAVETGAPMEEQNKLYNEMIEALTDKLQ
                     DDAREAARKEIATMNPYDAQLTAEAREFFNNIEKLPPKGIEKLFPQETIDRIFEDMVA
                     ARPLLQHIGLRNAGIRLKFLKSSRTGQAVWGKINAEIQGQLKQEFAEEEAIQSKLTAF
                     VVIPKDSEKFGPAWLQSYVSIQITEAFAAALEAGYLNGDGDKKPIGLSRTLTGTAESE
                     KVTYAEKQAQKKPLTFADSATVVKELTEVYKYHSTKADNTTPVAVEGNLVMVVNPVDA
                     WDVKKQYTSLNAQGVYVTAMPYNLILVESVAQTSGKVTTFVKGRYDAFVGGGIEFGRF
                     TETYALEDLNLYTAKQFAYGKAHDEKTAAVWTLNIGK"
     gene            23680..23970
                     /locus_tag="VW006_000511"
     CDS             23680..23970
                     /locus_tag="VW006_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEU9379418.1"
                     /translation="MEIEAKLHSLLDPFKERMRIFHSGEDANLSRMLESSQMVITRLV
                     GSGNIHDHQIRELILERARYVYNDQVEFFYENFKADILALSLDKIEMEDSDD"
     gene            23963..24151
                     /locus_tag="VW006_000512"
     CDS             23963..24151
                     /locus_tag="VW006_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379419.1"
                     /translation="MIKVLKEFYDLKEGVLRQVGERFEATEERFAELKEHLPGYVELE
                     KSSRSRSSKEVVSVNEEE"
     gene            24138..24527
                     /locus_tag="VW006_000513"
     CDS             24138..24527
                     /locus_tag="VW006_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9379420.1"
                     /translation="MKRSEGPSYRYKKPEYQNGDLRTPLTFYTSKIKKGVDGRDVSYE
                     KAFYTMGQTYSPSMKDVEIATGKAMKAKMTLKIRDPLTDYQPENRHFVEVGDIRLVGK
                     KWQIIDVRPDYDNRDFLIVLIGGGRDV"
     gene            24520..24918
                     /locus_tag="VW006_000514"
     CDS             24520..24918
                     /locus_tag="VW006_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379421.1"
                     /translation="MSSGVNLKGFDDILRNIEARLGEPVVRRNVNRALKETVEEFEPT
                     FKRAMAVYADTGKTVGAVVYGNVTGTSSGVPMVKLGFKSPRWTLIHLNEFGYAKNGHP
                     RGFGVMRRFFEASKPVFKSKVGMKLKQEFL"
     gene            24918..25298
                     /locus_tag="VW006_000515"
     CDS             24918..25298
                     /locus_tag="VW006_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379422.1"
                     /translation="MIKDKLTELYDALEEDESLSGISIKSFERPESLGDDETSIVIIP
                     VGPPMQTAHGSNTSLAKTFLYQINVESTNRVECKELQGRIEKIMEEQGFYQTEGGLEQ
                     WIPDIKRYVDVRTYRGMSALYEKY"
     gene            25315..25911
                     /locus_tag="VW006_000516"
     CDS             25315..25911
                     /locus_tag="VW006_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9379423.1"
                     /translation="MTVKGTALIGLKSVTIRVHDGQTPTVGKNLFTLEGKDNEGATQT
                     ARVTGLSSDPVKTYGSNVAYHVSNRGVGDVKVEMGLLDVPLALYTNALGYGDDDGIYY
                     FGADTVAKNVSILIESNTADGEPVYYGFYKGQLSMDAIDFETIKDKANELATTNVNFA
                     ATASSDTATKGRYGAIVYGSDAEKLKKLKGQLNMAAAG"
     gene            25960..26253
                     /locus_tag="VW006_000517"
     CDS             25960..26253
                     /locus_tag="VW006_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379424.1"
                     /translation="MAKVQFTIKNDKGEDVLKKSKEITTRDYRDYLVMNDSLTSDLSE
                     VEKLDKQLGFIASLFDDVTVEQLLEYTDFAKVISIFTDIYAHLVGDVDPKEKS"
     gene            26502..26732
                     /locus_tag="VW006_000518"
     CDS             26502..26732
                     /locus_tag="VW006_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379425.1"
                     /translation="MSRKEKARRRYWFWFTIFILYLAFGLYCICTNFGDTLGMLLLSP
                     FIFASLPLYAYLFLGLFIWAFMSLAMDDFYKE"
     gene            26789..30904
                     /locus_tag="VW006_000519"
     CDS             26789..30904
                     /locus_tag="VW006_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEU9379426.1"
                     /translation="MASGTPLGAMYIELGLDVSNFNPTLTGAKNAVKYFQNTVRSLDS
                     TLKGNEKNASLLQAKYKTLGQAIDSQRKVLDEMKKSFDKLEPGTAKFDKAAADIQREN
                     AKLAAMEGQLRGVEKALQDVGRENSWSGKMDALSEKFAKGGKKLRAMGDAMKPVSAAL
                     LAGFTLSTKKAIDFESQMNTTKSLLADTIPTADELNSTTQKLGESSKNWAKQYGISTA
                     SINEGMQEIIKKGFDANQTIEAMPAILDAAKASGEDFNTVMNATTNILQQFGLSTQDT
                     ERVTNSLTFVANKTAAGFADMGAAMEYVGPVAKNVGMDLEETAATVGLLSNNGIAGEK
                     AGTALRGALTRLLKPSKQNANAMAKLGFSAEEFRSGAIKLPDILDRIKKNTEGMTDAQ
                     KSALIATAFGTEAQTAMNILVDQGGDALRNLANETKGATTYTKDLANELSKSSKNGVE
                     RFKSSLEVLQINIGQKLLPLLTPVIEKVNQFIDWLSKAPPAVQSLAVGIGGFLALGYP
                     LLNFLGNGATALSILIKHAGKLGGLLKGGIGLAKVGSEATVLAEGAGVAASSSGLLSG
                     AIGALTNPIGLLVGGVALLAGGLVYLGHKKDEARIKAEEFGSQLSDTARKELRDFQSK
                     VDETSKAVANFGTHAGDAEKVSGAFKKLYDEIAAGAEKSNQRMQELTGKWGLSEEDLA
                     KAREKNAQMVSNAESMMNKINDIYQRHNGDASKFSQEEKEIILNNQREMIKTRISMMN
                     LSAEQQKAALQALNGEIGSLNETQLKHTRDVLKKSLDEEKQLYETSKSELKELLEGKV
                     IDQETYNKKLQTLETNHQQTMEALGTKYYQVMQNLDAKVKARTGQSWNYWEEAKKVLE
                     EYGLSYEVIGQKATEASQKMGESNSILAKYTSDMSKEIREANDAWSLLVGNINENGNF
                     EIKSNVKEVIGEATKSAEGWEQLQFIVKNANINSNARATIAEALVESGKWSTMTLEEK
                     QLIVQNQAGLQAIFDSENHLRIWNSMPAEVKQLLLENTDVMNKAEEASKALYNYDALT
                     PKQKELLATDENFRKAVARSTDTLTTWNATTPFTKDLKLDPTNVLNNGQLSIDKIMAW
                     NLSNPETKSLNATDNTSEAVDSAKASVNSPKQEKPIDLLAKDQTAGVRNETSAAINAI
                     KQNNPVDILAKNSTSSTVSEVKSGVNSIQDKTVTISARDNASGVLAGIRSWITRVTGD
                     FFTNVFASRHAHGTNYHPGGLAMVNDQRNSTYKELVTLPDGRSFIPEGRDVLLPLPKG
                     AKVLRADKTKRLMREMGVPKYAAGVGIPSDAKFLREMEEAQAKIVVQAKGETNSKDSD
                     KVVSEIAILRASMEKILTAILEKPSDTYLDADKISMSVYQRQGAIYAREGM"
     gene            30904..31602
                     /locus_tag="VW006_000520"
     CDS             30904..31602
                     /locus_tag="VW006_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9379427.1"
                     /translation="MFYMIINGFNTSTIPHCVVTDFGQVEAAKPRVVEDANLYGANGS
                     YRVLDGGYESYERTFSFYIPKLLDVSTIVEKFQPKDNVLEFSYQLGSVFYADFIGATY
                     SPHGMHAWKLEIKLNMQPFRYQKNVAPLIFAASGNINNPGSVYSEPVIEIEGDGDISL
                     TIGRTTMHLTVRRKVTIDCRHKKQNIYNADGAVQNTLRKRGGFFELAVGNNSLVFTGS
                     VRKVTVRPNWRYIL"
     gene            31599..33216
                     /locus_tag="VW006_000521"
                     /pseudo
     CDS             31599..33216
                     /locus_tag="VW006_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860112.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            37821..39742
                     /locus_tag="VW006_000522"
                     /pseudo
     CDS             37821..39742
                     /locus_tag="VW006_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841354.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            39723..39926
                     /locus_tag="VW006_000523"
     CDS             39723..39926
                     /locus_tag="VW006_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379428.1"
                     /translation="MNTEQLNQALQMTISEISTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            39929..40279
                     /locus_tag="VW006_000524"
     CDS             39929..40279
                     /locus_tag="VW006_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379429.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            40288..40587
                     /locus_tag="VW006_000525"
     CDS             40288..40587
                     /locus_tag="VW006_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379430.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            40591..40923
                     /locus_tag="VW006_000526"
     CDS             40591..40923
                     /locus_tag="VW006_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9379431.1"
                     /translation="MQQITEIIIAFATSFLTVAVGGIVKAVKDYLLRKGGEKAVIIAE
                     ILAKNAVHAVEQVASETGYKGEEKLEQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            40932..41888
                     /gene="lytA"
                     /locus_tag="VW006_000527"
     CDS             40932..41888
                     /gene="lytA"
                     /locus_tag="VW006_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9379432.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHWR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYWFDNSGEMATGWKKIADKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTMADKPEFTVEPDGLITVK"
     gene            complement(42026..42205)
                     /locus_tag="VW006_000528"
     CDS             complement(42026..42205)
                     /locus_tag="VW006_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379433.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(42347..42496)
                     /locus_tag="VW006_000529"
     CDS             complement(42347..42496)
                     /locus_tag="VW006_000529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379434.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            42777..43244
                     /locus_tag="VW006_000530"
     CDS             42777..43244
                     /locus_tag="VW006_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9379435.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            43277..43370
                     /gene="ffs"
                     /locus_tag="VW006_000531"
     ncRNA           43277..43370
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW006_000531"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(43453..44594)
                     /locus_tag="VW006_000532"
                     /pseudo
     CDS             complement(43453..44594)
                     /locus_tag="VW006_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(44649..45719)
                     /locus_tag="VW006_000533"
     CDS             complement(44649..45719)
                     /locus_tag="VW006_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEU9379436.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(45736..46116)
                     /locus_tag="VW006_000534"
     CDS             complement(45736..46116)
                     /locus_tag="VW006_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU9379437.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(46129..46392)
                     /locus_tag="VW006_000535"
     CDS             complement(46129..46392)
                     /locus_tag="VW006_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9379438.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(46392..46625)
                     /locus_tag="VW006_000536"
     CDS             complement(46392..46625)
                     /locus_tag="VW006_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379439.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(46625..46993)
                     /locus_tag="VW006_000537"
     CDS             complement(46625..46993)
                     /locus_tag="VW006_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379440.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            47564..47755
                     /locus_tag="VW006_000538"
     CDS             47564..47755
                     /locus_tag="VW006_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9379441.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            47778..47981
                     /locus_tag="VW006_000539"
     CDS             47778..47981
                     /locus_tag="VW006_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379442.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(48136..48303)
                     /locus_tag="VW006_000540"
     CDS             complement(48136..48303)
                     /locus_tag="VW006_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEU9379443.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            48323..48688
                     /locus_tag="VW006_000541"
                     /pseudo
     CDS             48323..48688
                     /locus_tag="VW006_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            complement(48908..49087)
                     /locus_tag="VW006_000542"
     CDS             complement(48908..49087)
                     /locus_tag="VW006_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379444.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(49229..49378)
                     /locus_tag="VW006_000543"
     CDS             complement(49229..49378)
                     /locus_tag="VW006_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379445.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            49683..50126
                     /locus_tag="VW006_000544"
     CDS             49683..50126
                     /locus_tag="VW006_000544"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9379446.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            50128..50643
                     /locus_tag="VW006_000545"
     CDS             50128..50643
                     /locus_tag="VW006_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9379447.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            50657..52018
                     /gene="radA"
                     /locus_tag="VW006_000546"
     CDS             50657..52018
                     /gene="radA"
                     /locus_tag="VW006_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9379448.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            52091..52588
                     /locus_tag="VW006_000547"
     CDS             52091..52588
                     /locus_tag="VW006_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9379449.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            52613..53396
                     /locus_tag="VW006_000548"
                     /pseudo
     CDS             52613..53396
                     /locus_tag="VW006_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            53541..54509
                     /locus_tag="VW006_000549"
     CDS             53541..54509
                     /locus_tag="VW006_000549"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9379450.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(54646..54756)
                     /locus_tag="VW006_000550"
                     /pseudo
     CDS             complement(54646..54756)
                     /locus_tag="VW006_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        16822 a         9234 c        12611 g        16089 t
ORIGIN      
        1 tccgccatag ctcagttggt agtagcgcat gactgttaat catgatgtcg taggttcgag
       61 tcctactggc ggagtaattg agttaagagg tttagacctc ttttttgtta tgtaataata
      121 atctattttg tgttatagta aaatgaacca aaaatagtac acaatgtggt ataatctttt
      181 tatggcatat tcaatagatt ttcgtaaaaa agttttctct tattgtgagc gaacaggtag
      241 tataacagaa gcgtcacacg ttttccaaat ctcacgtaat accatttatg gctggttaaa
      301 gctaaaagag aaaacaggag agctaaacca ccaagtaaaa ggaacaaaac caagaaaggt
      361 tgatagagat agacttaaaa actatcttac tgacaatcca gacgcttatt tgactgaaat
      421 agcttctgaa tttgcctgtc atccaactac catccactat gcgctcaaag ctatgggcta
      481 cactcgaaaa aaagaaccac acctactatg aacaagaccc agaaaaagta gccttatttc
      541 ttaaaaattt taatagttta aagcacctag cacctgttta gattgatgaa acaggattcg
      601 atacttattt ttatcgagaa tatggtcgct cattaaaagg tcagttaata agaggtaaag
      661 tatctggaag aagatatcag aggatttctt tggttgcagg tctaacaaat ggtgagttaa
      721 tcgctccaat gacttacgaa gagatggtga cgagcgactt ttttgaagct tggtttcaga
      781 agtttctctt accaacatta accacaccat cggttattat tatggataat gcaagattcc
      841 atagaatggg taagttagaa cttttatgcg aggagtttgg gcataaactt ttacctcttc
      901 ctccctactc acctgagtac aatcctattg agaaaacatg ggcttatatc aaaaagaacc
      961 tcaaaaaggt attaccaagt tgcaatacct tttacgaggc tcttttctct tgttcttgtt
     1021 tcaattgact atattagagg cgagacattt ttcggttctt tgtcaactgt agtgggttga
     1081 agaaagcgaa gatctagaaa ggacaaattt cgtcctttct tttttgaagt tttcaaagtt
     1141 cctaaaacca aaggcattgt gcttgataag tttgatgaga ttattggtgg cttccagttt
     1201 ggcgttggaa taaggtaatt gaagggcgtt gacgattttc tctttatctt tgaggaaggt
     1261 tttaaacaaa gtctgaaaca gaggtggaaa agcaagagct gatagagatt atagtggtgt
     1321 ttcaagtctt cggaatagct caaaagttta tctagaattt ctttattagt caagtgcata
     1381 cgaaaagtag ggcgataaaa tcgtttatca ctcagtttct gactatcttg ttgaatgagc
     1441 ttccagtagc gcttgatagc cttgtattca tgggatttcg gatgatggct tgtgttctgc
     1501 tctcaagaac agttatgata ttgagtttat caaagtcctg agcaataaag ctcatctcca
     1561 tctcccgatt gaaacagtca ctccccggac tgtttcaacg tcctaggaca taatctcagg
     1621 aagacgcgaa aaatcatgct caaagtgaaa attattgatc ttgcgaatga cagttgaagt
     1681 tgaaatagac aactgatgat caatgtcggt catagaaatc ttttcaatta acttttgagc
     1741 aatcttttgg ttgatgatac aaggaatttg atgattcttc ttgacgatag aagtctcagc
     1801 gagctccatt tttgagcaat gatagcactt aaaacggcct tttctaagaa gaattctagt
     1861 ttgaattttt ttatactaga aaatcagaac cataatacct atataaaaat attatagttc
     1921 taataggatt tacccaaaag ttttaaggcg gtctttttag aactttaatt gtttgaaatt
     1981 taggtagcaa atttgtttct attttgtcaa cttttcctat ttttatcttg ttgaggctgg
     2041 tattttaaca attcaggaat tgatagtgaa tgtgtaaaat tttttgttag aataagttta
     2101 taaaaaagaa aaggagtatt tgattatgtt acaaaaaatt tatgagcaga tggctaattt
     2161 ctatgatagt attgaagaag agtatggtcc tacatttggt gataattttg actgggaaca
     2221 tgttcatttt aaatttttaa tttattattt agtgagatat ggcattggtt gtcataggga
     2281 ttttatcgtt taccattatc gtgttgctta tcgtttgtat cttgaaaaat tggtaatgaa
     2341 tcggggtttt atttcttgtt gaggtaattt tagtaaattt ccgaactaat ttactctttt
     2401 atgtaaagat aatagtaaat agctagtaat ttttctaaat cattttttaa tagttggaaa
     2461 tagcaaatct ttctattgtt tcttcttgat aaaaaggcga ttttttatta taataaattg
     2521 taagatataa ttgcaggtga gagtcctgcc atgtatgtga gaaaggaaga gcctgagggc
     2581 tcagacaaaa ttatgacttc agttgttgtt gtaggtaccc aatggggtga tgaaggtaaa
     2641 gggaagatta cagacttcct ttcagcgaat gcagaagtga ttgcacgtta ccaaggtggt
     2701 gataatgctg gtcacacgat tgtgattgac ggtaagaaat ttaagttgca cttgattcca
     2761 tctgggattt tcttccctga aaaaatatct gtcattggga atggtatggt tgtaaatcct
     2821 aaatctcttg taaaagagtt gagctatctt catgaggaag gtgtaacaac tgataacttg
     2881 cgtatttctg atcgtgcgca tgttattttg ccttatcata tcgagttgga tcgcttgcaa
     2941 gaagaagcta agggcgacaa taagattggt acgacaatta agggaattgg tccagcttat
     3001 atggacaagg ctgctcgtgt tggaattcgt attgcagatc ttttagataa agatattttc
     3061 cgtgagcgtt tagaacgtaa ccttgctgaa aagaatcgtc tttttgaaaa attgtatgac
     3121 agtaaagcga ttgttttcga tgatattttt gaagaatatt acgaatatgg tcaacaaatc
     3181 aagaaatacg tgatagatac atctgttatc ttgaatgatg cgcttgataa tggcaaacgt
     3241 gtgctttttg aaggtgcaca aggtgttatg ctagatatcg accaaggtac ttatccattt
     3301 gttacgtcat caaaccctgt agctggtggt gtgacaattg gttctggtgt cggtccaagc
     3361 aagattgaca aggttgtagg tgtatgtaaa gcttatacga gtcgtgtagg agatggtcct
     3421 ttcccaactg agttgtttga tgaagtggga gaacgtatcc gtgaagtggg tcatgagtat
     3481 ggtacaacaa ctggtcgtcc acgtcgtgta ggttggtttg actcagttgt gatgcgtcat
     3541 agccgtcgtg tttctggtat tactaacctt tctttgaact ctattgatgt tttgagcggt
     3601 ttggatactg tgaaaatctg tgtggcctat gatcttgacg gtcaacgtat tgactactat
     3661 ccagctagtc ttgagcaatt gaaacgttgc aagcctatct atgaagagtt gccaggttgg
     3721 tcagaagata ttaccggagt tcgcaatttg gaagatcttc ctgagaatgc gcgtaactat
     3781 gttcgtcgtg tgagtgaatt ggttggcgtt cgtatttcta ctttctcagt aggtcctggt
     3841 cgtgaacaaa caaatatttt agaaagtgtt tggtcctaag agatttttaa gatttgttta
     3901 agataggtcg ggtatactat agacagttac aagaagacct cctaacttgt tgtaacaaat
     3961 atcctaaact tttctttttc ataataatct cccttaactc caccgaatca ggtggagttt
     4021 tttagctcta tttcaggctt ttggggacta ttctaaaaat aatttttcga tatttttcgg
     4081 tatttttcgg attttggtcg gggaattggc ggggactttt tgagattttg gtggggattt
     4141 ttttagcgaa tatgactaag aaataggtct gttgtcgctt cagcaagttc gtcctcaact
     4201 tggttataac gatccgtcat ataaaccttt gtatgcccca gcgcttggct taattgttca
     4261 agcggaaccc ctgcaataat gctttgagtc gtgaagaagt ggcgcatcat gtgaggtgtt
     4321 acatgcaatc ctgttgcttc attcactaga ttgaagtttc tatttaactg gtttggattg
     4381 atgagaccac ctttctcgtt gatagttata taatccttgt gctgttcctt gataatccct
     4441 aactttcgct taatcttaga agcttcagct atcagataat agataaggtc tgttccgata
     4501 tcatcaaggc agacatatcg ctctgaatcc ttcgttttaa gcccgccttt ccctttcaag
     4561 gtctggttgc ttcggctgtc tctaagatgc agtatagccc gtccgctgtc gttctgagtg
     4621 atgtccattg gacgcaaacc aaagacttct cctcttctca atccaaaaat ggtaagatag
     4681 gtcagagcgt agaattgttt tggcataatc tcttctgcct ttgctatcca agtcttgaac
     4741 tctttgagag tcactttctt gtttgcagca gggatatcac tctggccgat gaaaacacct
     4801 ttcaagcgat ttgagagcag atttccattt ttgacggcat cattcagcaa tgccatgaag
     4861 ctggaattga gggtttgaac agtgtatctg gtatggttct gcaacttttc agcgataaag
     4921 agttcatact catttctatc caaattttta agcagggtag aaccaaactt tgccttgata
     4981 tggttcttgt agagattatc attgaggtag taggaagtgt cattccagcg ccctgttgac
     5041 aatctctttt cagaataaat atcccaatat tgatcaagtg tcagattcgt attgatacct
     5101 aattcttgtt cttggatttg ttgctcaagc tctactaagg ctgcacgagc ttgtggaagg
     5161 gttgtgagac cacttttact tttttctctt tttttacctc ggaagaaaaa agaacgtctg
     5221 acatagtatc gcttgccttt ggcagtctca tagtaaaaga tatttgggta ttttgtttta
     5281 ttatatttca ttgtattctc cttgtttatc ggcttctgga caaggtctaa acattgagaa
     5341 tattgacatc acccctttca tggtgtaaaa tagggtatag aaaagaggcc tttttaatgg
     5401 ctgatttttt ataagggtaa gcttcacaat caaactttgg cgagggagat tgtggagctt
     5461 ttttgttaat tactcctcaa gcaatcttat aaaatcattt tctgtcatga tttcaatatc
     5521 ttgacctttt tctaacaagg tttgtgcttt tttcatttta ctacttaacc catctgtacc
     5581 gactactctc caatcttgtt ctcctacaac taagatatta gtgtgtttag tcactccttt
     5641 ttcaggaata ccaccaacta atgcagtagc tttgttagct tcttttctag tcattcgctc
     5701 gagttttcca gtaaagcaaa agtacaaacc gtaaaagtaa tggtctgggt tcattgctgc
     5761 cttttcttct tctgttggct gatagataag attttctttg tactgataac tttttttcct
     5821 tttaaatcca tactgaccaa gtaagccagt tttattgtag ctatattctt ttaaaaaagc
     5881 gttgaggtcg gaaaatgaat tagtagataa taagtattct aaaatcaatc cgcttgctcg
     5941 tgcatctgat aaagcgttgt ggtggtctag ctcaatattc aaatttttag ctagtctttt
     6001 tagtttgtaa ttcaattgtc cagggagagc gaccttggct aatcgatacg aacaaatata
     6061 ttctatatta tcaaaatcca gctcgtattt ttggtataca tctttaagag cacccatatc
     6121 aaactgtgca aagtgggcta caactatatc agaaccaata aaatcaacaa tcgcctttct
     6181 cacctctggg aatgtaggtg aatcaagaac atcttcaggt gtaatgccat ggatgaaaat
     6241 attgaaatca tcaaactctt cttctggatt gattaaagtg taaaaagcat caacaatatt
     6301 tccatcttta aattttacta atccgataga acaaacacta ccgcgaaagt cattcgcagt
     6361 ttcaacatct aaagcaacat acgagtaaga catatgagtc tcctttcact ccatcaatgc
     6421 aaagtattcc tcttttacca tgacttcatt cgtcatggtt tttagattgt agtaggacat
     6481 gaatttgagg taatcaaact ctgtggggtc gtctaagctt tctatcgcgt cttttacgag
     6541 atgatggatc atattcctat cagcttcgtt ttcacagcgt aggcgagcgt tctggtactc
     6601 tgagcgtgtg tggtctttgt gtccaagttc atgcagtagt actttaactc tctctttctt
     6661 gctgagtttg ttagacaaga aagctgtatt ggtttctttt tcgtaaaatc caagttcatc
     6721 aggcatcaaa tctccatcaa aatcgataat acgaatctga aaatgactta taatttcttt
     6781 ttcagtcact aagcagtacc tctaatcacc agcttctttg agataacctt caatgataga
     6841 ctggatgatt ttcttctttt catctgttaa ttctcgaccg ccaaacatca tgacattaga
     6901 tgccatttct tcaacgttta gagtcttacc ttcccatttg tagtcatgac taccagcgat
     6961 tgttgggtta tccgtgcgac caagtaaata atctgtggac acgttgaagt agtcagcaat
     7021 cattgaaact cgttcaacat ttggtgtgga tttcttcatg ttataaattg tatttctgct
     7081 aaaacctagt ttttcttcaa gtttatttaa tgaaatacct tgtttgtcag ccaattcttt
     7141 tattttttca aatgtgaaaa acattgatac atcaaccttt ctaaggcatg acaaaaaata
     7201 tttaataaat ttactacaaa actgttgaca aagtttaata aatttactac aatagttttt
     7261 gtaagctaaa gagttagcga acaagacaac taaaaaataa agcctaatga aactgattgg
     7321 cgtccgtttt ctaggtataa ccttactttt tagtaggtct tttctctatg ttttgatttt
     7381 aataaattta tttatcaatg tcaagaaatt cgctaacttt ttagataatt ttttaaaagg
     7441 aggtgaggaa atgagaccaa gacgatatcc gtatagtggg aaaaaagagt ctaccctcgt
     7501 aaaggcagac cctgaattag tagaaaaaat tctaagaaac actagttttc ttgagcgttt
     7561 acaagttctg ttagcaacga aaccgtagtt tctgtactga gtttatgagc gacaaggacg
     7621 ctattaagaa tagcctttct aatttccatg tcgattgggt attctttaaa tacctcatct
     7681 aacatatctt ggataactgg aatagcatcg ttgataattt cttgagaaac ttgtaaaaca
     7741 tcttctttag tgagttttga catataaaat ttcctccttt ctattggaat tttgactaaa
     7801 acggtgagag gtcctagtca agattattat agcaatttag gaagatatta cctcagtctt
     7861 gagactgata taggaggttg aatggaagat aaaatcatcg aacttgctga ttacttcatc
     7921 agcgagaaca caacgtacag agaagctaaa atagcgtgtg agaagctatt taaacaagcc
     7981 agccatgaga tagaactcag agcgctggaa agtgaaacga agaaatagaa aggagaaaga
     8041 tgagtaaaga actaaagata atcaaggcta aaatcaaaac tcgtttgatt gagttggata
     8101 tgactcaagc tgaattggca aaacaagtat ctgtagcatc atcagttatt tcagagttgc
     8161 tgaagtatgg caaaggtagt gagtctgtta aggaaaaagt tgcagatgtt ttgggtattg
     8221 aaaacccttg gagaaatcac tgagaggtcc atacatgcaa gcgaaaataa tactgaattg
     8281 gcagaagaaa aatcaccaac ttagtcagat gatgatcgat agtcttgagg gactagatgt
     8341 ttgggaaact attttaacac taggaaaagt aagaagagga atattatgaa cgaaattttt
     8401 aattttcacg ggcaggaagt ccgtactttg acaattgatg acgagccttg gttcgttggg
     8461 aaagatgttg cagacatcct aggatatagc aaggctagaa atgcgattgc tcttcatgtt
     8521 gatgaagatg acgccctaaa acagggcctc acagataatt taggaagggt ccaagaaact
     8581 attatcatca acgaatctgg tctctactct cttatcttat ccagcaagtt gcctcaggct
     8641 aaagagttta agcgctgggt gacatcagag gttttgccag ctattcgcaa gcagggcggt
     8701 ttcattcgcg aggacttgga cgaggatgcc tttatcgctc tattcactgg tcaaaagaaa
     8761 ttgcgtgagc aacaggttac catgcttgaa gatattgact acctcaagag tgagcaaccg
     8821 attcatccta gctatgctca atcattactg aagaagcgca aagctcgtgt cgtggcttgc
     8881 ttaggtggta ttgatagtcc agcttatgcg gataagactt tcgctcagtc agtctttaga
     8941 caagctgaga ttgatttcaa agaccacttc aacattagtc gctatgattt gctaccgaaa
     9001 aagtttgcag atgcagcctt ggcctactgg atgacgtggg agccaagcac aaacactaag
     9061 atgaaaatca tgaaattaaa ctcatttgat gacgtctaga aaggggaaga agatggacaa
     9121 tgttctactt tcactgtctg aatggattaa atctattatc aaggacacaa tcacaaggct
     9181 agtcgaaata gagaaagata gtgatcacta ttcagagttg atggatgtga acactacctg
     9241 cgaatttcta ggaattaagt atgccacctt ttcagataat tatcgttact taaagggatt
     9301 tccaaaggaa ttacctggta agaaatggtc aaaaagagcc atcaaagaat ggctctctaa
     9361 tcaaatataa taactttact aaaaggcttc tggacaaggt cttagcaaaa ttatttgact
     9421 atattatagc acaaaaagag gataaggaga taaaaatgtt tgaaccaccg attttagacc
     9481 agttgatggg ggttggagcc ttactgcttg gatttgcagg ggcttgccgt catatcaaat
     9541 tacaggaaca acgcaaggaa gaagaaagac gagaagagca agaatttgcg tctatgatta
     9601 ttcaaggcta taaccatgct tacgaacgtg gtagagaagc tgaacgtcaa gaaatccgca
     9661 agaatattcg tcgtccgttc aagggcttta cctacgacaa tgaaccgcct caaggattgc
     9721 gtcctgagcc gttagctttg ccagaaccta aacagtctgc aatcagattt ttgtaatgag
     9781 gaggtcagga aatggaagaa ttgattgaat ggcttgaaac caaagaaaaa ttaaacaggg
     9841 aaatgatttc tgacgaggaa acatcggact ttaacctaca tctagaaaat gataatgata
     9901 ccatgttcat gatcaaggaa tacttgaacg attataacaa gctagccaaa gactatcgaa
     9961 ttctatcgtc tgaaaatcgt ctacttaagc ttgaaaaaat ggagctggaa ggcaggcaca
    10021 tctatgagga tatgcggatg aagtaccgtg ccaatcgtag gaagtggggt gctcggtatg
    10081 tctgaaatta agtggataaa aatcacaacc gatatttttg acgatgaaaa gatttgcctg
    10141 attgatgcct tgcctgatcc tgatgccatc ttagtgatat ggttcaagat tttgacactt
    10201 gctggaaaac ataacagtaa tggtttgttg atgatgactg ataaggttca ctatacagat
    10261 gaaatgttag ctactatttt tcgtagacca ttgaatacag taagaatggc tattggagtt
    10321 tttgaacagt ttgggatgat tgagattatc gatggtatta ttagcttgcc aaattgggaa
    10381 aaacatcaaa acgttgacgg aatggagaaa atcaaggaac agacacgtaa ccgtgtagcc
    10441 aaataccgta aaaaacagaa aaatcttgct cttggtaacg ttacaggtaa cgttacagta
    10501 acggacggta acgcactaga agaagataaa gataagaata agaatagatt agataaagat
    10561 aagaataaga aaagaataac tactactagt agtggtagtg aagaaaatat cttagaactt
    10621 tttcaatctg agtttcgtag actcttatct ggatttgaaa ttgaagaaat caaccatcta
    10681 ctaaatgaga atgatgtgga tttggtgaaa gaagcattga agactgctat taactcagga
    10741 aagccgaaca tcaaatatat tggtgggatt ttaagaaatt ggcagatgaa caatgttacc
    10801 actgttgaac aggttcgtca atcggaaaag aagaacaagg ataagaaaga agaacaggag
    10861 gccaaggacg aatgggggta ctagaactaa ttgaacaatt cgagattgac tattatccgt
    10921 taagctacga gaagaaaact cttttagcag accaaccaat tcatcaagtg gttgcctgct
    10981 tgtctgaaat ggctagctgg catgaatgcg gaggtcggct agtatggtag acaatgtgtt
    11041 tgaggaaatc gccttatctt atcgtaggaa tacagaacaa caagaagagt tctgcgaaaa
    11101 gcataacatc cctttgataa agatattgag gaccgagagt gttgtatgtc gcatgtgtga
    11161 atctgagcgg attcatgaag aaaatcagga aagagtgaat gaactggcta atgctgagaa
    11221 tgagcgagag aggaaatact atcttgaaaa gttctctctt tatgatgaag ttttgaaaaa
    11281 tgctactttg gacaattttg aaacaccaac cgaaaaagaa gcggaaaagc tagcttttgc
    11341 aaagaggatt tgtcgtgagt ggtctgaggg tgctaggaac aacatcgtgt tacaaggaga
    11401 agctggaaca ggtaagagcc atttggcttt tgctatggtt aaggctctat ctgagtacac
    11461 gaaagagatt gctattttta ttaacgtaac tgacttgctg atgaagatta aagctgattt
    11521 tagtcaggaa gagtttctgg tcaataaaat tgctagtgct aagttcttgg ttttggatga
    11581 tttaggaatg gaaaaagata gcgaatggtc atttactatt ctctacaata tcttgaataa
    11641 gcgttcaaac acaatcatta ccacaaattt gatttctgcc gatattcaaa aaagatatgg
    11701 cagacctttc atgtccagac taatgaaggg tgtagataaa gaccatttga tggttttcaa
    11761 tgatttgaca aacaagcgaa agcaatattt ttagaatagg ggtggctgat gtttatttta
    11821 aagcatggga caagagagga taagccgttt ctaaggtctg caattatcag tgtgactgga
    11881 ttggacattt catgttctga ggagaagaaa gccatgcggt ttgtttctcg ggcggctgcc
    11941 gtacaggttg gaaaggcttt gaggggttcc tttgggaact tttaccctgt tgaggtggag
    12001 tgatgatgaa attcttaaat ggtgactgca tggacatcat gaaacaatat cctgataatt
    12061 actttgacct agctattgtt gatccaccat atttttctgg tccagaaaaa aggaaatttt
    12121 acggacggaa aatcagtcca ataggtgtaa gcagactcta tggcgaaatc tcagagtgga
    12181 aaattccaaa cagagattat tttgatgaac ttttcagggt atctaaaaat caaattattt
    12241 ggggtgtgaa ctacttcgac tattcttttg gttctggccg tattgtgtgg gacaaagtta
    12301 atggtcaatc aagtttttca gattgtgaga tagcgtactg cagttatcat gatagtacta
    12361 ggctatttcg ttacatgtgg aatggtatga tgcaaggtaa atcaatctct gaaggtcata
    12421 tacaacaagg aaataaggca ttgaatgagg ttagaatcca tccgacacaa aaaccagtca
    12481 acctttacct ttggttattg caaacttacg caaaagaagg cgataaaata cttgatactc
    12541 acgtcggttc agcaagtagc ttgattgctt gcggagagct aggatttgac tatgttggat
    12601 gcgagattga taaaagtatt ttcaacctcg ctcaacagag acttgatgct tatgagaagc
    12661 agttgaagtt attttaggag gtattgatgt tagagcttta cttcgtctat aacgggcact
    12721 gcaagtttta ccttgggagg tttgacaatg tcgatgatct cattgagcag atggaagatc
    12781 atcagtgggc tttctcggct atcactcatc caagatttca gaagcacatt ggtcagcgga
    12841 cgacacgatt tgactacggt tctaaggatt gttactattt agcgactttt tcaggaggag
    12901 aaaaaaatga ttgaacttat taaagaattt ggaatggcta ttctgtggtt atttctcggc
    12961 tatttagtcg gggaacgtgc agcaagaaag gaaaagaaag atgatcaata acgttacatt
    13021 tttagtggga ggtaagaaac atggttggag taacctatca ggaaattcat ctctttgttg
    13081 aatttttgaa agagcagtac gggcaaggtc gtccagacta tattgaagcc ctgaacgact
    13141 tagacggtct ggtggaagtc tcctacagag aagctattga aagattttta gaagatgaag
    13201 tacgataaac aggctgagat tgacggactg aaacgcacaa tcgagcaaaa cgaagagaag
    13261 ataatcgagt attcgaagcc gtgtgatgca cgcaagagac ggattagagc gctggagcgc
    13321 gatttgttga agaaaaagaa taaagaatta agacggaaag cggaggagtt ggaagatgat
    13381 ggaagagtta aagaaaaaag ttaatgcagt atacaactgg acggtagaag acgggaagcc
    13441 gcaacctccc cagcaagatt taccacaagc agtgaaagac cgggcggact atttttggga
    13501 aatggcagaa gatggtatga cgtttatggg agcgatggaa tgcatcttcg ctgatgaaaa
    13561 gcctacagac tatgatttgg gagctactaa gggttggttg ccaaaatcta aggagtttga
    13621 tgattgggtt ggctattcgc caggaatgtc tcaggtagtt attgcagttt atttgattta
    13681 tggaggaaac taagatgaat aagcaggaat tgattgagaa atataaaaaa cttgagggtg
    13741 tatggaatgc tgaaggagca gaactagctc gtcaaatttt tctgcaagac ttggaacaac
    13801 tagataaacc aaaaccagtc aaagttccgc agtgtgtggc ggaatatata gaatttaaaa
    13861 agaaaaacaa ttttcatgtt tacggtgcaa tgagagtaat tgaagatcat tatgataaga
    13921 aagttcctga gtggttttac gaaaataaca tcgaaaaatt ctgtcttgct tggcttgacg
    13981 gctacgaggt tgaaaaagag aagcggtatt ttgttaagat taaagggaat attaaagaaa
    14041 atatgttggt ttatggagaa cttttgaaaa ggtatttctt tacaaaaagc tttagtttag
    14101 acgatgttat atattcccac acccgtaaag aactagaaga cgcaaacttc ggctgggtgt
    14161 ttgattgtga aggaattgat atagaggagg tggaaaatga ataaagatct aattgaaaca
    14221 ccacgcttta acttttttat aggggatgaa gttctcttga aagggaaaat agtcggtttt
    14281 gatgtagatg agaacaagtg cgttgaaaat gttgttagat tggaatacgg gcaaactctc
    14341 aatgtaccca acaataatat ttatattaca gacgacatcg ttgataaatc caaaattaaa
    14401 gtcgtagtac cgcagtttgt ggcggattgg tatgaagaga ataaggattc ttttgaattt
    14461 aatgtttgtg attggattgc tttcagggat gaggctaaaa aatcagaaaa tagagagttt
    14521 aataattgga ttaataatag cagagaaaat cctattcaaa ccctcgtcaa catgaaccaa
    14581 ttcggctacg aggtcgagga agagaagcgg tatttggtga ctttaaaaaa taggcagcct
    14641 ttggtcaaat cgcaatcagg gagtactctt tattttagtc aagatataac agctaggaat
    14701 tataaaggta ctcaaaaaga actagaagac gcaaacttcg gctgggtgtt tgattgtcca
    14761 gggattgaga ttgaggaggt ggagtgatga gccttacgct aaatagcaca attggagact
    14821 tagttttggc aatcggagaa attatcgttg gttctgatgg taaaaccact acagcgatac
    14881 tggagatacc tgatcaaagc ttttacttag agattgagct taaattgaag gaggaggtca
    14941 taaattgaaa cgattcatag ctatctggat tctgctatct gctggattga atatctggca
    15001 gagtatctac attaaaaagt tagaagaaaa gcgccctatt gtcgtctata aagctgataa
    15061 cgcaggcgct gagatattcg gtaaagtcgt cgagaaagga cggcatggca agctatacac
    15121 gcttaccatt cgtgactacg gggtgttcgt ggttacgaag gacgtgtatg agaaagtgaa
    15181 agttggggat gaggtgatgt tgtgaaattc ttggatttat tcgcaggaat tggcggtttt
    15241 cgtttaggaa tggagtctgc cggccataaa tgtattggct tttgtgaaat tgacaagttt
    15301 gctagagaaa gctataaagc gatacacaat acgaaaggag aaatagaatt acatgacatc
    15361 acagcagtat cagatgaatc tattcgagga atcggaagtg tggacattat ctgtggagga
    15421 tttccgtgcc aagctttctc aattgcagga aacagacgag gttttgaaga tacacgagga
    15481 actttgttct ttgaaattgc taggttcgca tctattctca gacctaaata tctattcctt
    15541 gagaatgtta aaggattgct caatcacgaa aatggagtta cattcgagac cattatctca
    15601 accttggatg aactggggta caacgtggaa tggcaagtgc ttaacagcaa gaatttcgga
    15661 gtcccccaaa atcgggaacg agtgttcatt atcggacatc ttagaagaga acgtaccaga
    15721 agaatttttc ctctcagcgg aaaaaatcag tcaactagta accaatcagt catgaaaatt
    15781 gggaatataa acccatctgg caacggaatg aatggggaag tctatcaagc tgatggtcta
    15841 gctcccacgc taacaacaaa caagggagag gggcaaaaga tagcaataaa aagcaatact
    15901 ataaaacaat ttggggtatt gcaacccaat tttaatcaat gtggagtggt ttacgaaaca
    15961 gatggcatcg caccaactat cagagcatat caaggcggag gacttgagcc taaaattatt
    16021 caacgtggtc atggttataa tcaaggcgga gaacatgaca tcgctcccac tctgacaagt
    16081 aatagatatc atgaaaataa tcatttatca gatggctttc gaatcagaaa gctaacacct
    16141 aaagaatgct ggaggttaca aggttttcct gattgggctt ttgacaaagc gcaagaggtc
    16201 aactctaaca gtcaattata taaacaagca ggaaatagcg tgacagttaa tgtcatagca
    16261 gcaatagcaa aggagttatc atgaacgcac tagaaaaagt cgaacaatgg tttatcgacc
    16321 gtgaaatcaa agaccgaaaa ggtcgctgga ttgatggctc gtttgtcaaa gaggaggatt
    16381 tggcatgata ccgaaattta gagggttatc cattgacgaa aaaagcaaag gagaatggca
    16441 atacggacat ttaattgaag atagaggaag agcatttatt atcagcgaag tggtagaagc
    16501 caatgaacaa tacattacta taggttcttg gtgtcctgta aatccagcaa cattaggaca
    16561 atcaacagga ctcagagaca agaacggcaa ggaggtattc atcggtgaca tcgttaaatg
    16621 tacaagagga tgtctccatg aagtatattt agaaaaagaa tacggtggca catacatagg
    16681 tggaatgcct gctgtatacc taaaaggatt tggagatgga tatgcgtgga cggaatatga
    16741 ggaaatcatc ggcaacatct acgaaaatcc ggtcaggagg taagatttgg caatagacat
    16801 caaaaaaaga ttgaaggctt tgccttatat cgatatcaaa gctaagtcaa agcaccaaga
    16861 aatcatcagt ttgaagtcag gtattttacg aggacagcag ttcgacagca tgccaaaatc
    16921 aaaaagtaac aagaatcaaa ctgaagaatt gaatgtgttg attattgaca agtcagaaca
    16981 gctatataaa gaaatcaaac aaatgtacca cgaacgtgac gaactcgttc aagcgattga
    17041 gtcgctcgat gatccagtgg aaaacatcgt gatgcgatta ttgtacattg acggattatc
    17101 ttggaaagaa gttcaaataa aactaaattg tagccctgcc accatccaac gagcaaaaca
    17161 taaagcgtta ctaaaattat ctaaaatgta tgataagaat gatagcaaat gataatttta
    17221 atgtggtaaa ttagtatcat gaagaatagc agagaggaaa cctctgcttt tttttgcatt
    17281 aaaaggaggt gaggatatgt ggtagttgtt gaaccaatcc gaaatagaga tgatgttcag
    17341 cttatgattg aatggctggc gttgcatagt gcagtcaaag agtcagatag acaacgcaac
    17401 ctcatgctct tcctttctgg tgttaatttg ggatttcgta ttggtgatat cgttaaactg
    17461 aaagtaaagc acgttaaagg ttggcatgtc cagatcgtcg atgaaaagac agacaagcca
    17521 accaaacgaa agatgccaaa gaaattcaag aatgctatga ggcagtacat aaaagataag
    17581 aaagatgaag acttcctctt tccaagccga aacggaaagc atcagcatat aaaacctaac
    17641 acagcttata agattatcaa aagagctgct gaagaagttg gtctggaaaa catagctact
    17701 cactctatga gaaagacctt tggtttattc atgtacgaac aaaccaagga tgtcgctctg
    17761 ataatggacc tactgaatca ctcaagccag agtatttcac ttcggtatat cggcaaaaat
    17821 caagattcac aagacagagc catgacgaaa tttcagggct tttaattttt ttattttaac
    17881 atcaattcat tgttttaagg ttatgatgat ttcatttcgc acatgcaaga taaacgcttg
    17941 ataaagctga gttaaaactc atgtagcgag ttcactagaa tatgtaaaac aaggaattga
    18001 aagagtaaaa acaaaggaga ttgcacagtt atgaaaggta ttattaaaag actttttaat
    18061 aaaagaacca ctaaacaaaa accattagga aaaattgtag ttggagtcga aattgaaaat
    18121 cgctcagaat taaaagagtt aactcaacaa tgttgtgaag caatcgaaca cttgaacaat
    18181 tgtattgaca agctaaataa atttgagctc aaagtatcaa catcaataat caaatgattg
    18241 aagtttcaac tcgagaagag cgcaaccagt tttacaattc cagcgagtgg agaacgataa
    18301 gaaggcaagc acttaaacga gatcattacg aatgtgtatg gtgtagggat gaaggcaagg
    18361 tcacgactac taatttagaa gttgaccata tcaaggagct agagttatat ccagagttcg
    18421 cgcttgatat cgataaccta cgaacattgt gcaaagcatg tcataataaa aggcatgatc
    18481 gttttgataa gaatgacaga aatttccgaa aagatgaatg gtggggttag gtgaacgaat
    18541 cttaaacacc ccccggtcaa aaaaatcagg aaatttcaaa ggagccagta agcggtctgc
    18601 actcgactgt ccaaattttt aacaaaaaat taaagggggt ggggggtaat ggaagaatac
    18661 tcggaaaaaa atataaaaga attagaaaat cagctacttt ccaaaatcgg ctatttcagt
    18721 ccgagaaaaa aggatgcgat ccagtacgaa aaagtgaatc gttatcttta tcttgtcaga
    18781 ttgctatatg agctgaaagc taaacttcat gaagacggat tggtcatcac tgttcacaat
    18841 gggcaacaga gattccaaaa agcgaattct ctcatcaagg aaatcaacac aacaagcaat
    18901 cagcttttgg ctattgagcg atcgtttgat tttgaggttg aaaattctcc tgttgagaaa
    18961 cccacgtctg gaagtgacct gctatgattt ctcatccctt ggttgatgac tacatcaaaa
    19021 tggccgagag tggagaaatt gtcgtcaaca aggaaaggaa gttgctgttt aaaatcatca
    19081 aggagaaaat ctatcctcgt gatgatttgt attttgataa tgacttgatt gataagttca
    19141 ttcgttttgc ggaaaagaat tttttccctc tggccaagta tcagcttttc ttgactccgt
    19201 ttatctttct ttttcggaag gaagacgggg agccacactt cgacgagtat ttgtacacac
    19261 ttgctcgtgg gggtggtaag aatggtttta tgtctgccag atcctcgttc tttatcagtc
    19321 ctatctaccc tatcagagat tatgatgtga ctatcactgc caattctgag aaacagggca
    19381 aggtttcatt tgaggaggtc tatgagacta ttcaaaggcg tggccttgag gaccatttct
    19441 atctaactaa aatgtctatt acaggtcgag cgaacaactc ggtcttttct tttcggacga
    19501 ataatccgaa gaccatggac tccgctcgtg atggctgtct tgagtttgat gagattcacc
    19561 agtttgaaga tgataaggcc gtgaaggttc aacggtcagg tctcggtaag attgctcatg
    19621 ctcggacttt ctacaacggt acgaatgggt atgtgcgtga gggattttat gacaagctga
    19681 tagagaagtc tatgcaaatc ttgaacggag aggttgatga tttcaggctc tttcctttca
    19741 tctgtaagtt agacagtgcg gatgaagtgg atgatatgaa gaagtggcca aaggcaaatc
    19801 cgatgttgga tgaaagcact ccttacgcta agaggttact tgcaagaact aaagctgact
    19861 atgatgacct tgagttggaa ccatctggtc gtcaggagtt tatgactaaa cggatgaacc
    19921 ttcctgaagc tgaccttgag aaagatgtca cctctcgtga aaagttagtt gcttgtttgc
    19981 gttcacctgg tattgacttg ttaggtaagt cttgtgttgc agggtttgac tatgctagta
    20041 ttcgagactt tgcaagtgtt ggtttgttgt ttaagaacgg ggatgagttt atctggaaac
    20101 aacattcatt tgctcgtaaa gtgtttttga aagctttcaa actaaaagcc cctattcagg
    20161 aatgggcaga taaaggtttg tttacgattg tggacggtcc tagcattgat ccgcggctgt
    20221 tggttgcgaa gctggaagaa tggagtaagt tttatcagat tgaacttgta tgtgctgatg
    20281 gtttcagaat ggacttgtta aaaccgcttt tggaagaggc tggttttgaa tatgagttct
    20341 tgcggaatcc tggggcgatt caatctaagg ttgcgccaat cattgaagac ggatttgcca
    20401 atgagcggtt tgtctttgag aacgataact ctatgatttg gtatacggat aatacctata
    20461 tcaaagagga taaggatggc aataagcgtt tcttgaagaa agagccggtc agaagaaaga
    20521 cagatggttt ccatgccttg attgctgctc tatacaagag ggagctagta catgagtcaa
    20581 acgttgggga attccttgac atgctcgata gctgggactt ttaatctaag aataaatttt
    20641 gggtgggtgg tcggcaaaaa taagaggaag gaggggaagc atgggattct taaacttatt
    20701 taagcgtgat gcgcctgatg ttggttttga ctttgaagag ttggaacgaa tgttgggacg
    20761 cttgcaattg aagagtttag cgattgataa gtcggcggag tttgtggctc gtatctttgc
    20821 taagtcggaa tttcggtata tggtggagaa tcatcatgtg acgtcagact gggattatat
    20881 tttaaatgtg cgtcccaata agaatgagtc ggcctcagag ttttggcaga aggtcattta
    20941 tcgcttattg acgaaaaacg aagtacttat tgtcttgtct gatgataagc agttactggt
    21001 agctgatgct tttactcgta ggcgttatgc tctgtatgat gacacctttg agatggtgtc
    21061 tgtcggggat tatacatttc agaggaagtt tgctatgagt gatgtcatct ttcttcagta
    21121 taataacaat cgcttgcaag aatatgtatc tgacttgttt tctgattatg aaaaactaca
    21181 tactcgtctg gtggaagcct tagctagaaa taatcagatc cgtggggttc tggctacaaa
    21241 agcgaatgga acttttgatg aggataagag aaagaaactg cagagatatg cggacaatct
    21301 ttttaagtcc tttacgtcta agacggttgc cattgtaccg acaatggatg gtgtggagta
    21361 ttcggagttg accaatacgg taggaactac aaatctatca gttgaagagt tgaagaaact
    21421 gagacgtcaa tttgatgacg aggttgcgga tatattaggt ttgccgacgg ctttgatgca
    21481 tggagatatg gctgatttgt ctaatagcca gaagatgttt aacagttatt gttatgaatc
    21541 gctggttaaa aaagtgggtg acgctctaaa ccatgctatg gtgagttcgg ctggatacgc
    21601 taatcagaaa aaatttgtga tttttggtga ggggggactg gataagtttg ctctggctga
    21661 gaacatagat aaactcatct catctggggc tatgactcga aatgaggtga gattggaact
    21721 tggtcttgag gctgttcctg gtggggattc tttccttatc actaagaact atcagggtga
    21781 aaacgaaata gagaaaggag gaaatgagga tgacaatcat tcagattaag ggagcgattg
    21841 tatccaatga tgaccgctgg ttttatgatt ggatagacat ggatgcaact gctcctaagg
    21901 atattgtatt gccgacgacg ggagaagatg tggaggtgca catcaattct ggtggtggtg
    21961 atgtgtatgc tggtagtgaa atctatacag cattgcgtgc ttatcagggg catgtgacgg
    22021 tgaaaatcgt ggggattgct gctagtgctg cgagtgttat cgctatggct ggtgatcagg
    22081 tggaaattag tcctacagcg caaatcatga tccataatgt atctgctggt gtacgtggtg
    22141 accataaggc gctcttgcat gaggctggtg tgttagaagg gtttaataag tccatcgcat
    22201 atgcctatat tgataagact ggtaaggcat tagatgattt actggatcta atggagggtg
    22261 aaacgtggtt tgatgctcgt tctgctgtta atcatggctt cgcagataag gtcatgtttg
    22321 aaagtcagga agctcctgtc ttggtagcta gtcagacacc tgtgattcct cgtgatttta
    22381 tcgaaaaaat ccgatctgcg atgaccccag atgtggagtt attggcggac ttggttcgtg
    22441 ataggttgaa taacagttta cttatcccag aagataagaa aaaagaagac aagacggctg
    22501 aacctgtggg gttgggtcgt ttcccatttt aagaaaggaa ataaaaaaca atgatgaaac
    22561 ttggtaatga atttacaact gctcgtcaga actttatgaa tgcagtagaa actggtgctc
    22621 ctatggagga gcaaaataag ctctataacg agatgattga ggcattgaca gataagttgc
    22681 aagatgatgc tagagaagct gctcgtaaag aaatcgcaac tatgaatcca tacgatgcac
    22741 agttgactgc tgaagctcgc gaatttttca acaacattga gaaacttcct cccaaaggaa
    22801 ttgagaaatt gttccctcag gaaacaattg accgtatttt tgaggatatg gtggcagccc
    22861 gtccacttct tcaacacatc ggcttgcgta atgctggtat tcgtttgaaa ttcctcaagt
    22921 ctagtcgtac aggtcaagcg gtttggggta aaatcaatgc tgaaattcaa ggacaattga
    22981 aacaagaatt tgctgaagaa gaagcgattc agtctaaatt gactgctttt gttgtaatcc
    23041 ctaaagactc tgagaaattt ggtccggctt ggttgcaatc ttatgtctct attcaaatta
    23101 cagaggcctt tgctgctgct cttgaagctg gttatttgaa tggtgatggg gacaagaaac
    23161 ctattgggct gtctcgtact cttacaggga ctgcagaaag tgaaaaagta acttatgctg
    23221 agaagcaagc tcagaaaaaa ccattgactt tcgcagactc tgctaccgtg gtaaaagagt
    23281 tgacggaggt ttataaatac cattctacaa aagctgacaa taccactcct gttgcagtag
    23341 aaggtaattt ggttatggta gtgaaccctg tagatgcctg ggatgtgaag aagcaatata
    23401 cgtctctcaa tgctcagggt gtatatgtaa cagctatgcc ttataacctc atcttggttg
    23461 agtctgttgc tcaaacttct ggtaaggtga cgacgtttgt caaaggtcgc tacgatgcct
    23521 ttgtaggtgg tgggattgag tttggacgat ttaccgaaac ctatgcttta gaagacttga
    23581 atctctacac tgctaagcaa tttgcttatg gtaaagcgca tgatgaaaag actgctgctg
    23641 tgtggacact gaatattgga aaataaaagg tgactaagga tggagattga agctaaactt
    23701 cattccctcc ttgatccttt taaggagcgg atgagaattt ttcatagcgg agaagatgcg
    23761 aatctctcac ggatgttaga aagttctcaa atggttatta cccgcttggt aggaagtggg
    23821 aatatacacg atcatcaaat aagggaactt atcttagaac gtgctcgcta tgtctataat
    23881 gaccaagttg aattttttta cgaaaatttc aaagcggata ttttagcatt atcgttagat
    23941 aagatagaaa tggaggatag tgatgattaa ggtattaaaa gagttttatg acctcaagga
    24001 gggagtcctt aggcaggtcg gagaaaggtt tgaagcgaca gaagaacgat ttgctgagct
    24061 aaaggaacat ttacctggct atgtcgagtt ggaaaagtcg tccagaagtc gtagctctaa
    24121 ggaagtagtg agtgtaaatg aagaggagtg agggaccaag ctatcgttac aaaaagcctg
    24181 agtatcaaaa tggagacctg agaactcccc tgactttcta tacctctaaa ataaaaaaag
    24241 gggttgatgg tcgtgatgtg agttacgaga aggcttttta tacgatgggg caaacttatt
    24301 ctccaagtat gaaagacgtt gagattgcga ctggaaaagc gatgaaagct aagatgactt
    24361 tgaagattcg tgaccctctg acagactatc agcctgaaaa tcggcatttt gtcgaagtgg
    24421 gggatatccg tctagttggt aagaaatggc aaatcattga tgtccgtcct gattatgata
    24481 atcgggattt tttgattgtc cttatcggag gtggtcgtga tgtctagtgg agttaatcta
    24541 aaaggatttg atgatatttt gaggaatatt gaggctcgac taggagagcc ggtagttcgt
    24601 agaaatgtca acagagcctt gaaggagacg gttgaggagt ttgagccaac tttcaaacgg
    24661 gctatggcgg tgtacgctga cactgggaag acggttggtg cggtcgttta tggaaatgtg
    24721 actggtacat ccagcggagt tccaatggtt aaattaggtt ttaaaagtcc tcgttggact
    24781 cttattcact taaatgaatt tggctatgca aagaatgggc atcctcgtgg tttcggagtc
    24841 atgcgtcgct tttttgaagc gagtaagccg gtctttaagt ctaaagtcgg catgaagtta
    24901 aaacaggagt ttttgtaatg attaaagaca aattaactga actctacgat gctttggaag
    24961 aggatgagtc tttatctggt attagtatca agtcttttga gcgtcctgag agcttgggag
    25021 atgacgagac cagtattgtc attatccctg tagggcctcc aatgcagacg gctcatggta
    25081 gtaatactag tttggctaag acttttctct atcaaatcaa tgtagagtct actaatcgag
    25141 tggagtgtaa agaactccaa ggaagaattg aaaaaataat ggaagaacag ggattttatc
    25201 agactgaagg tggtttggaa caatggattc ctgatattaa gcgctatgta gacgttcgga
    25261 cttatcgtgg tatgagcgct ctatatgaga aatactagaa agaaggaaaa agaaatgaca
    25321 gtaaaaggaa ctgcacttat cggacttaaa tcagtcacca ttcgtgtgca tgatggacag
    25381 actccaacgg ttggaaagaa cctctttacg ctagaaggta aagataatga aggggctaca
    25441 cagaccgcta gagtaactgg attatctagt gaccctgtaa agacttatgg tagtaatgtt
    25501 gcttatcacg tctccaatcg aggagtcggg gatgttaagg tagagatggg cttgcttgat
    25561 gtcccattag ctttatatac gaatgcttta gggtatggcg atgatgatgg tatctattac
    25621 ttcggtgcgg acactgttgc taagaatgtt tccattctca ttgaaagtaa tactgctgat
    25681 ggggaaccgg tttactatgg tttttataaa ggtcagcttt ctatggatgc tattgatttt
    25741 gaaacaatca aggataaggc taatgagtta gctactacta atgtgaattt tgctgcgaca
    25801 gctagttcag acactgcaac taagggtcgt tatggtgcta ttgtctatgg ttcggatgct
    25861 gaaaagttga agaaattgaa gggtcaacta aacatggctg ctgcagggta gaaggagggc
    25921 gaaagctctc ttttttaccg tttttagaaa ggaaagtata tggctaaggt tcaatttaca
    25981 atcaaaaatg acaagggaga agatgtccta aaaaagagta aggaaatcac aactcgtgat
    26041 tatcgtgact atttggtcat gaacgattcc ttgacaagtg atttatctga ggtagagaag
    26101 cttgataagc agttgggatt tattgctagt ttgtttgatg atgtgacagt agaacaatta
    26161 ctagaataca cagattttgc aaaagtgatt tctattttta cagacattta tgctcatctg
    26221 gtaggtgatg tagacccaaa ggaaaagagc tagcacctgg gcaagctatc caacgttttt
    26281 atggctttat caagcaagtt acagagggtc cttatggaat gagtattcgc gacgtgatgg
    26341 atacgagttg ggaggacttg atgggcgttc ttggaagtat gaaaacgagt gaacaagaag
    26401 aagtggttga cttggcagat tttttagaga gtctctaagt caggacttta caaataaaag
    26461 acttaggaat ataatagagc tatataaggg aggtggaaga tatgtctcga aaagaaaagg
    26521 cgcgtagaag atattggttt tggtttacca tctttatcct ttatcttgct tttggcttgt
    26581 attgtatttg tacaaatttt ggagatacct tgggtatgct tttattatca ccctttatct
    26641 ttgcttcctt gccgctctat gcttatctat ttcttggttt gtttatctgg gcttttatga
    26701 gcctggcaat ggatgatttt tataaagaat gatgaaaagt ccgtaagggc ttttttcttt
    26761 gctctgagga gttaggaagg agaacaaaat ggcaagcggt acgccgttag gagcaatgta
    26821 tatcgagcta gggctggacg tgtcgaattt caatcctact ctaactggtg caaaaaacgc
    26881 tgtaaagtac tttcaaaata ctgtccgttc tttggacagt actttaaaag ggaatgaaaa
    26941 aaatgctagc ttacttcaag cgaaatacaa gactttggga caagccattg attcacaacg
    27001 taaagttttg gatgagatga agaaaagttt tgacaagctt gaaccgggga cagctaagtt
    27061 tgataaggct gctgctgata ttcagcgcga gaatgccaag ttagcagcaa tggaaggtca
    27121 gctacgtggt gttgaaaaag ctttgcaaga tgtagggcgt gaaaatagtt ggtctggtaa
    27181 gatggacgcc ttgagtgaga aatttgctaa aggtggaaaa aaattgcggg ctatgggtga
    27241 tgcaatgaag cctgtatctg ctgctttatt agctggtttt acactttcga ctaagaaggc
    27301 gattgacttt gaaagtcaga tgaatacaac taaatctctg ttagctgata ctattccaac
    27361 agcggatgaa ctgaacagta ccacacaaaa gctgggggag agttcaaaaa attgggcgaa
    27421 acagtatggg atttctactg catctatcaa tgaagggatg caagaaatta tcaagaaagg
    27481 ttttgatgcc aatcaaacaa tcgaagccat gcctgctatc ctagacgctg ctaaggcgtc
    27541 tggtgaggat ttcaatacgg ttatgaacgc cactacgaat atcttgcaac aatttgggct
    27601 atctactcag gatacagagc gagtaaccaa tagtttaacc tttgtcgcca ataaaacggc
    27661 tgctggtttc gctgatatgg gggctgcaat ggagtatgtt ggtcccgttg ctaaaaacgt
    27721 tggtatggac ttggaagaga ctgcagctac tgttggtctt ttgtccaata atggtattgc
    27781 tggtgaaaag gctggtacgg ctcttcgtgg agctttaaca aggctgctga aaccatctaa
    27841 gcaaaatgca aatgcaatgg caaaacttgg tttttctgca gaagaatttc gtagtggtgc
    27901 gattaagcta cctgatattt tggatcgtat caaaaagaat acggaaggaa tgacagatgc
    27961 tcagaaatcg gccttgattg ctacagcctt tggtacagaa gctcagacag cgatgaatat
    28021 cttggttgac caaggtggag atgctttgcg taatcttgct aacgaaacca agggggcgac
    28081 aacttatact aaggatttag caaatgaatt atctaagtct tctaaaaatg gtgtggaacg
    28141 ttttaaatct agtttagaag ttttacagat taacattggt cagaaattat tacccttact
    28201 aactcctgtg attgagaagg tgaaccaatt tatcgactgg ttaagtaagg ctcctccagc
    28261 cgttcagagc ttggcggttg gtattggtgg ttttttggct ctaggatatc ctttattgaa
    28321 ctttctaggc aatggtgcga cggctcttag tattttgata aaacatgctg gtaaattagg
    28381 aggactttta aagggcggta tcggtcttgc taaggtagga agtgaagcca ctgttttagc
    28441 ggaaggagct ggtgttgctg catctagttc tggtctcttg tctggagcta ttggggcttt
    28501 gacaaatccg attggtttgc tggtgggtgg tgttgcttta ctagctggag gattggtcta
    28561 tctagggcat aagaaagacg aggcgcgtat taaggctgag gaatttggtt ctcagttgag
    28621 tgatactgca aggaaagaat taagagactt ccaaagtaag gtagatgaaa ccagcaaagc
    28681 agttgctaac tttggtacac atgctggaga tgctgagaaa gtctctgggg cttttaaaaa
    28741 gctttatgat gagattgctg ctggtgccga aaagtctaac cagagaatgc aggaactgac
    28801 tggcaaatgg ggtctcagtg aggaagacct cgcaaaagct agagagaaga atgctcaaat
    28861 ggtttcaaat gctgaaagca tgatgaacaa aatcaatgat atttaccaac gtcacaatgg
    28921 agatgctagt aagttttctc aagaagaaaa agaaatcatc ttgaataatc aaagagagat
    28981 gataaaaact agaatttcaa tgatgaactt gtcagcagag caacagaagg ctgcgcttca
    29041 ggctttgaat ggagaaattg gtagtttaaa cgagacgcaa ttgaaacata cacgagatgt
    29101 tttgaagaag tctctggacg aagaaaaaca actttatgag acttctaaaa gtgagctgaa
    29161 agagttgctg gaaggaaagg ttattgacca agaaacctat aacaagaaac ttcaaacttt
    29221 agaaacaaac caccagcaaa ctatggaagc cttagggact aagtattatc aggttatgca
    29281 aaatcttgac gctaaggtaa aagctcggac tgggcaaagc tggaactact gggaagaagc
    29341 gaagaaggtt ctggaagaat atggtttatc ttatgaagtg attggtcaaa aagctacaga
    29401 agcttctcaa aaaatgggag aatctaatag tatccttgcc aaatacacta gtgatatgag
    29461 taaggaaata agagaagcga atgatgcttg gtctttactt gttggtaata ttaatgagaa
    29521 tggcaacttt gagattaaat ccaatgtaaa agaagtaatt ggggaagcga ctaaatctgc
    29581 tgagggttgg gagcaattgc aatttattgt taaaaatgcg aatatcaatt caaatgcccg
    29641 ggcaacgatt gctgaggctt tggttgagtc tggtaagtgg tcaactatga cactggaaga
    29701 aaaacagttg attgtccaaa atcaggcagg actgcaagct atctttgata gtgaaaacca
    29761 tcttagaatc tggaacagta tgcctgcaga agtcaagcaa ctccttttgg aaaatacaga
    29821 tgtgatgaat aaggctgagg aagcttccaa agctctctat aactacgatg ccttgactcc
    29881 taaacagaaa gagttgctgg ctacggatga gaatttcaga aaggctgttg cgcgttcgac
    29941 tgacacgcta acgacttgga atgcaaccac accgtttaca aaagatttga aactagatcc
    30001 tacaaatgtt ttgaataacg ggcaattgtc aatagataaa atcatggctt ggaatctatc
    30061 taatccagag actaaatcat taaatgctac tgacaatact agtgaagcag ttgatagtgc
    30121 aaaagccagc gtaaactctc cgaaacaaga aaaaccaatt gatttacttg cgaaagatca
    30181 gactgcaggc gttagaaatg agacgagtgc ggctattaat gcgattaagc aaaataatcc
    30241 agtagatatc cttgctaaaa atagcacttc cagtactgtt agcgaggtca aaagtggcgt
    30301 taatagtatt caagacaaaa cggttacaat cagcgctcga gataatgctt ctggtgtgct
    30361 tgcaggcatt aggtcttgga ttactagggt aactggtgat ttctttacga atgtttttgc
    30421 tagtcggcat gctcatggta ccaactacca tccaggtggt cttgctatgg tcaatgacca
    30481 acgcaatagt acctacaagg aattagttac tcttccagat ggtaggagct tcattcctga
    30541 ggggagggat gttcttcttc ctcttcctaa aggggctaaa gttttgcgag cggataagac
    30601 caaacgcttg atgcgtgaga tgggtgttcc taaatatgct gcaggagttg gcattccgag
    30661 tgatgctaaa ttccttcgtg agatggaaga agcacaagct aaaatagtgg ttcaagccaa
    30721 aggagagaca aatagcaaag acagcgataa agtcgtgtct gagatagcga ttctgagagc
    30781 aagtatggag aaaatcctta ctgctatcct tgaaaagccg tcagatactt acctagacgc
    30841 tgataaaatt tcaatgagcg tctaccaacg tcaaggtgca atttatgcca gggagggaat
    30901 gtaatgtttt atatgattat caatgggttt aatacatcaa ctatccctca ctgcgtggtg
    30961 acggattttg gtcaggtgga ggcagctaaa cctagggtgg ttgaagatgc taacctctac
    31021 ggagccaacg gaagttatcg ggtgctggat ggtggctatg agagttatga acggactttt
    31081 tcgttctata ttcctaagtt actggatgtt tctactatcg tggagaaatt tcagcctaag
    31141 gacaatgtgc tagagtttag ctaccagttg gggtctgtat tttatgcgga ttttattggt
    31201 gcaacctata gccctcatgg gatgcatgcc tggaaactag aaatcaagtt gaacatgcaa
    31261 ccgttccgtt atcagaaaaa tgttgctcct cttatctttg ccgcaagtgg aaatatcaac
    31321 aatccaggct ctgtctatag cgagcctgtg attgagattg agggagacgg tgatatttct
    31381 ttgactatcg gaaggacaac tatgcacttg accgttagac gaaaagtgac cattgattgt
    31441 aggcataaga aacagaatat ctataatgca gatggcgcgg ttcaaaatac tttacgtaaa
    31501 cgtggaggct tctttgagtt ggcagttggt aataacagtc tggtctttac tggttcggtt
    31561 cgcaaggtca cggttcggcc gaattggagg tatatcttat gatttatctt actgaaggca
    31621 atacaccttt aaatgaggcc tacaatgatg aaattgtcca tttggggaac aatacctatc
    31681 aactgacctt tcgttttcct acatcggata ccaagtggga attactgaaa gaggaaactt
    31741 ttctgactgc agatgacctg catggtgagc aggattttta tatttttgag gttgaaaagc
    31801 aacaaggata tattcaagtc tatgccaatc aggttatcag tttgttaaat aattacatcg
    31861 ttagctctat cgaggttgac cgtgtcagtg ggacaagggt gttgagtgct tttgctggta
    31921 gtattaccag agccaatcct ttttctttct tctctgatat tgatgatagg catacgctca
    31981 atatcaagga taagaatgcc atggaggtct tggccaaagg caagcattct atccttggtc
    32041 agtggggcgg agatatggtg cgaaacggct acaatttacg cttgttgaag aatggcggtt
    32101 ctgaaaatga atcgcttttt atgtacaaga aaaacttgtc cagctaccag cataagacct
    32161 caacgaagtc tttaaaaact cggataacct ttaaaacgac tgttaagggc gagggagaaa
    32221 atgcggttga ccatgattat atggtggtga ttgacagccc cttacttggg aactacagcc
    32281 aaatctacga agatgtggtg gaagtcaatg accaagacgt gacagatgag gctagcttga
    32341 ttgaatacgg taagcagtat tttcggacaa gtatgtgcga catgctagaa gataaccttg
    32401 aaatctcggt tgtcggtcag agtgatgttg cggtgcagat gtttgatgtg gtcagtttct
    32461 accatgagtg gtacggtctt gatgttcgta agaaaatcac caaatatacc tattcgccaa
    32521 tggcaaaact cctaaaatca ataggttttg gaaccttcca gtccagtctt gcaaatgcga
    32581 tcggtgggat tgtaaatgat gccgttttga atgaaagccg aaatctgcat cagatttttg
    32641 aagaacgttt gaaaaaggaa atcgccaacg ctgaccgtgc ctttgacgct gaatttttca
    32701 agcgtgagaa aaccatcacg gatgccatcg aacttgccaa ggccaaagcg gaagaagtca
    32761 agcaagaact gtctgacact atcaatcagc gctttaatag ctttgacaac gggccattga
    32821 aagaagctaa gcgcaaggct gaggaagctt tgcgaaatgc tggcgcaagt agttctcttg
    32881 ctcaggaatc caagcggatt gggctggatt ctgttgctag acttgaagcg tttaagtcgc
    32941 agactacgag cgcacaaacg gctctgtcag gtgacttgga cgctctgaaa cggactatcg
    33001 tgaatgatat tcgaccgaag caagcacagg ttgaagctga gattgccaag caagttgaag
    33061 cacttgttca gaccaaaaaa gaactggctg gcgcaagtac cctgcttgca caggaagcta
    33121 agcggattga gctggattct gttgctagac ttgaagcgtt taagtcgcag actacgagcg
    33181 cacaaacggc tctgtcgggt gacttggacg ctctgaaacg gactatcgcg aatgatattc
    33241 gaccgaagca agcacaggct gaaactgaga ttgccaagca agttgaagca cttagccgga
    33301 ctaaaaatga actggctggc gtgaagtcag cgcaagcgac gtatgaggag acgacgactc
    33361 gtagactgtc agaactgacc aacttggcca atggtaaagc cagcaagtca gaactcacgc
    33421 agacagctga ggagctagct agtcggattg cgagtgtaca ggcatccggt cgaaatctat
    33481 tcttgaactc actattcaag caggatattc caaaaacagg aatttggaca acgagtacat
    33541 atacggctac tatcgatagc gaaagtaagt atcttggaca caaggctctt aaaattatag
    33601 gtttgaatcc atctggccgt gatggaggta atcccaaggt tacttatcca gctctgggtc
    33661 aattcgggaa agtaattccc ggaagtacga ctaatcaaga tgtaaccatt agtttttatg
    33721 ctaaggcaaa taaaaatgga ataatgctga gatctcgatt agggaatatc ggatataaaa
    33781 ctggaaatgt gacattgtcg acagaaatta agcgatatgt tgtccatatt ccaaaaggtt
    33841 ggacaaacga atccaagcag accacaaatg aatggttgtt caatttcaac caggaaggaa
    33901 ccgtttggat ttggatgccg aagttcgaaa taagcgatgt agatacttct tattcagaag
    33961 ctcctgaaga tgtcgaaagc caaatctcaa cagtcgaatc gacctttaaa cagcgtgctg
    34021 attcactcga cgctggtgtg aatcgtctga ctgaaggcct tagaaccaaa gtggatatca
    34081 gcgcactcaa cgtgactgct gagaatatcc ggcaatctgt gaagagtctt gagacagaca
    34141 cgcagaacaa gctaaatcag aagttgagtc aggctgaatt tgaggtgcga gccggctcta
    34201 tccgtcagga aatcctgaac gcaaccaagg ataaagccag caagtcagaa ctcacgcaaa
    34261 cagctgagga actggctagt aagatagcga gtgtgcaggt cgggggtagg aattatatcc
    34321 ggggtacaaa gcgcatgatg ctagccagag gattgtgggc atcaggtact tttagaccgt
    34381 caggcgctgg gacggcaaag acgattgatg tatcagacag tccagcaact ggctttgata
    34441 aagcgatacg attgacctca agcaatgcta gagaccaaat aggtattgct caagatggat
    34501 ttcatatctc gcaaggcaca tacacgatgt cttgttgggt caaaggcaga agaggtcaaa
    34561 aggtcaagct acaaacttat tggcaagtca atgataattc gggtatttcg cccatcttta
    34621 cattaaagga tgaaaattgg acaaagctat cgtttactag cgctagaaat agggctggag
    34681 tcgcatcaat tggctatgtg tatctcgtaa atgctgaagt cggagaatat ttagatgttc
    34741 ttgcgcccca gctggaagac ggaagtttgg caacaagctc aaaagaagct cctgaagata
    34801 tagaaggtca gatttcaaca gtagaatcga cctttaaaca acgagccaac tcgctcgacg
    34861 ctggtgtgag aagcttgact gaagggctta gaaccaaagt ggatatcagc tcactcaatg
    34921 tgactgctga aaatatccgg caatctgtga agaggcttga gacagacacg cagaacaagc
    34981 taaatcagaa gttgagtcag gctgaatttg aggtgcgagc tggctctatc cgtcaggaaa
    35041 tcctgaatgc aaccaaggat aaagccagca agtcagaact cacgcagaca gctgaggagc
    35101 tagctagtcg gattgcgagt gtgcaggcat ccggtcgaaa tctattcttg aactcactat
    35161 tcaagcagga tatttcaaaa acaggaattt ggacaacgag tacatatacg gctactatcg
    35221 atagcgaaag taagtatctt ggacacaagg ctcttaaaat tataggtctg aatccatctg
    35281 gccgtgatgg agggaatccc aaggttactt atccagctct gggtcaattc gggaaagtaa
    35341 ttcccggaag tacgactaat caagatgtaa ccattagttt ttatgctaag gcaaataaaa
    35401 atggaataat gctaagatct cgattaggga atatcggata taaaactgga aatgtgacat
    35461 tgtcgacaga aattaaacga tatgttgtcc atattccaaa aggttggaca aacgaatcca
    35521 agcagaccac aaatgaatgg ttgttcaatt tcaaccagga aggaaccgtt tggatttgga
    35581 tgccgaagtt tgaaataagc gatgtagata cttcttattc agaagctcct gaagatatag
    35641 aaggtcagat ttcaacagta gaatcgacct tcaaacaacg agccaactcg ctcgacgctg
    35701 gtgtaagaag cttgactgaa gggcttagaa ccaaagtgga tatcagcgca ctcaacgtga
    35761 ctgctgaaaa tatccggcaa tctgtgaaga gtcttgagac agacacgcag aacaagctaa
    35821 atcagaagtt gagtcaggct gaatttgagg tgcgagccgg ctctatccgt caggaaatcc
    35881 tgaacgcaac caaggataaa gccagcaagt cagaacttac gcagacagct gaggagctct
    35941 ctagtaagat agcgagtgtg caggtcggag gaatcaacct cttgcgtaat actgcgagtt
    36001 tgttgattgg cgatcgttca aaaggatgtt ggatgagtgc aagcggagga aatgggcgag
    36061 cgattagcgt agaagttttg gatcctccca aaaaaatgat aaaaaacatg attcgtgtta
    36121 ttgaaaatac gaatggtgga aataaagatt taactcaact tgttagattg cgaattggtg
    36181 aaaagtacac gatttcttgt tatgcaagga ttgctagcga tagcccaaat gcaaacgtga
    36241 acttgctatt tcgttcgtgg gcaaataata ccgatttaaa tcgcaaattt cagaaatcca
    36301 tctctcataa aaattggcaa aaatattcat ttacatttac tgctgatgca attgaaaatt
    36361 caattcaatt tggacaatct ggcgcaggaa ttatcgaaat atgcgctccg aaaatcgaat
    36421 caggaacgtt agcgactgat tacagcgaag ctcctgaaga tatagaaggt cagatttcaa
    36481 cagttgaatc gacctttaaa caacgagcca actcgctcga agctggtgtg aatcgtctga
    36541 ctgaaggcct tagaactaaa gccgatatca gctcactcaa tgtgactgct gagaatatta
    36601 ggcagtcggt gaagagtctt gagacagaca cgcagaacaa actaaatcag aagttgagtc
    36661 aggctgaatt tgaggtgcga gctggctcta tccgtcagga aatcctgaac gcaaccaagg
    36721 ataaagcaga taagacttta gttgtatctg aagctgggaa attgcgtgaa gaattttcaa
    36781 aaatgaaggt gggaggacgg aatctatgga taaaatccaa gacggttgga gctgtaattg
    36841 aaaaattacc tgaaaaccac atcacaggtc aaaaagaatg ctataggcta gagaacaact
    36901 ctactttaac gttcaacctt gaaccagatt tcagctcaag gttgtatcaa aaagttactt
    36961 ttagcgcttg ggtcaagtac gaaaatgtag tccaaggtcg aaatttttgg aatgtattta
    37021 attgcttcaa acattatctt tttagaaaaa atagtgagac cggagtacag agtggtccag
    37081 attatgctac gcttggtatg tataaaggtt cggcagattg gaaatatatt acattcactt
    37141 atgactactc tgaaaaaaca aattttgatc aattgaagac atcattgcga ttcaatcttg
    37201 aaggtgctac aagcggtaca gcttgggtaa caggaatcaa ggttgaaatc ggtagtgtgg
    37261 cgacggactg gagtcctgcg cctgaggacg ctgatggtct catcactgaa gctaaggcta
    37321 cctttgagcg gacagctcag ggcttgcgaa ccgatttatc agctattcag gaatatgtaa
    37381 ataaagacgg tcagcgacag gaagccctac agcgctatac tcgtgaggag agcacgagac
    37441 aagcgacagc agtccgtgag ctggtcaatc gtgatttcgt tggtaaggct acttatcaag
    37501 aagatgttaa gggtatcaat caaaggattg aagctgttaa aactagtgcg aataaagaca
    37561 tcgctagtca aatcgctagc tatcgtcaat ctgtagatgg taagttcacg gatatttcaa
    37621 gtcagataac tacttataag caagatgtgg gcggtcaaat cagtggcctt tcaaatagac
    37681 ttacaagcag tgagcaagga accactactc agatttcaaa tctttcaaat cggataaaca
    37741 gtaataaaca aggcacagat aatcagattt caaatttaaa gactcaggtc gctacaaaca
    37801 aggataatgc tgaacgacaa atgggtagaa tatctgatca ggtttctgca aacaaagcga
    37861 atgctgatag tcaatttgcg aatgtgacca atcaactagt gcgaaaagta gagactactg
    37921 acttccagcg tgttaaggaa accagtaaac tttacgagcg gattttgggc aatactgaaa
    37981 atggaattgc ggataaggtt gctcgcatgg ctctgaccaa tcaactgttt caggttgagg
    38041 tggctaaaaa tgccagcaat ggtcaaaatt tgttgaaagg cacaaaagac ttctcagggg
    38101 gttggaaaaa caagggtgca aattggaaaa agcacgctga aaaatacaaa ggtgttgatg
    38161 tcctatttaa aaataattcg tggaatggcg ttggacaaga gattgatgca aaaattggtg
    38221 aagtctatac attcagctta tggatgaaga gcgactggaa gaatgataca gtaaatttct
    38281 atgtaaatag aaatggatct gttgagaaag gttggggtgt tccatctgaa acatcggtcg
    38341 ctataacaag tgaatggaaa cgttactcat ttaccttcaa aattactgtg gacggcttca
    38401 tctttcctcg tgtagaacga cttaatcaaa atacaaatct atatattgca ggtcttaaac
    38461 ttgaaaaagg atcgtatgca acaccgtaca ccgaagctcc tgaagacacg gacgaagcga
    38521 ttcgctctgt tcaaagccaa ctaactggct catgggcagt tcaaaacatc aactcggctg
    38581 gagatatcat ctctggaatc aatcttggcg ccaatggaca taaccgcttt gttgggaaat
    38641 tgacccacat cactggagag accctgattg acagagcagt catcaagtct gccatggttg
    38701 ataagctcaa aacggccaat tttgaagctg gttcggtcac gactacgata ttagacgctg
    38761 aagcggtaac tgctgagaag ttgaaagttg acgatgcgct tattagaaaa ttaactgcaa
    38821 aagatgcttt tattgaccaa ctgatatcta aacgtatctt ctctattaag gttgagtccg
    38881 tcatttctag ctcaaccttc ctagaagcct atcaaggccg aattggtgga ttcacacttg
    38941 gtcaatttga ccagggtggc ggtcgctgga tttcaggtgt caatcagttc tctgttggta
    39001 tggggaatgg tgccgggcat ggagtccgga cagccttctg ggcgaactgg ggaaataatt
    39061 ggaactatgc cggacctaaa gcatggaacg tcaatactga tgggaaaatg tactgtagga
    39121 atgaagtcgg tttttatgat caagtggatt tttcgaattc atcgagagca aacttctatg
    39181 ggaatactac tttttctcgt tctcctgtgt tttcaaatgg tatcgaactt ggaagtaaag
    39241 atgtgcttgg tgatggttgg aatcccaaag gcggaaggaa tgcggttgtt tggtggaatc
    39301 aggtcggtag cggtagcgtg aagtattgga tggaacaaaa atcagacaga cgcttaaaag
    39361 agaacatcac agatacagct gtgaaagcct tggacaaaat caacagatta agaatggttg
    39421 catttgattt catcgaaaat aaaaaacatg aggagattgg tctaatagct caagaggctg
    39481 aaaccatcgt tccaaaaatt gtctcacgag atcctgagaa tccagatggc tatctgcata
    39541 tcgactatac cgctttagtt ccttacttaa tcaaggctat tcaagaatta aatcaaaaaa
    39601 tagaaaaaat ggagaaaaca atagcatgaa taacaacatg ttgaccaata tcgcacttaa
    39661 agcaatttag gagcttgctc ttgaaaatag aaaacgaaca cacagattgg agaacttaga
    39721 aaatgaacac agaacagctt aaccaagcct tacaaatgac aattagtgaa atatcaacaa
    39781 cttcaacaaa ttcgatgatt acaagtaata tcttgagtat tcagttgaat gagcaaaggg
    39841 aagagaatca aagacttcaa gcacgagtgg atgagctgga agctctgctt gatgaacaaa
    39901 ctaaaccagc agacaaagga gaatagacat ggcagaaaca attcaaaaca cagataactt
    39961 actagacctt acaaaaatca cagaaccatt tgatcttgcg agtgctttgc gctacatgaa
    40021 agaaaatgga gagttcattc gttgcaagaa tgtaagcgat gacttctata tgtatcgtga
    40081 cgttcaaaaa cgtcctgtga tcgtaaatgg ccgtcgccaa ttcaaggatg ttgaaaccgt
    40141 ttgggcattc aatcagtggg gtggtacaat cgcaacaatc aacgtagccg ttctgttgaa
    40201 tcatgaattc tatatcatga aatttgatgc agagggcaat cctgactgga cggttccaac
    40261 ggtagaacct aaagaatagg aggttgtatg ccaattgaag aagctgaaaa aatcgctcaa
    40321 agtcaggtag cttgggcgat tttgtttatc ttgcttttct ttattatcat tcgatatctt
    40381 atcaagactt cggacaagcg agagaagaag attatggatt tgcacgagca atcaaaggcc
    40441 gactctaata gacgagaaga gcgtttgatg actcacctag aaaagaccac tacagaatta
    40501 accacaatca ctcacacggt cggagacatt caaaaagaaa tggttcgcat gaacgaccgc
    40561 atggaagaaa tcgaaaaagg agaataacaa atgcaacaaa ttactgaaat catcattgct
    40621 tttgcgacaa gctttttaac agtagcagta ggcggtattg taaaagcagt aaaagattat
    40681 cttttgcgta aaggcggaga gaaagcggtg atcatcgctg aaattctagc taaaaatgca
    40741 gttcatgccg ttgagcaagt agcttcagag actggctata agggcgaaga aaagctggag
    40801 caggctcgtg ctaaagtccg tgctgagctt acaaaataca atattagtat gactgacaaa
    40861 gacttagaca ccttcgtaga gtcagcagtg aaacagatga atgacgcatg gaaaggacga
    40921 taggataggg aatggatatc gatagaaaca gactacgaac aggcttgccc caggttgggg
    40981 tgcagcctta tcgacaagta cacgcccact caacaggcaa ccgtaactca acagctcaaa
    41041 atgaggctga ttaccactgg cggaaagacc cagaattagg ttttttctcg cacgttgtcg
    41101 gaaatggccg tgtcatgcag gtaggacctg tgaacaacgg aagttgggat gttgggggcg
    41161 gttggaatgc tgagacctat gcagcggttg aactgattga aagccattca actaaggaag
    41221 agtttatggc tgactatcgc ctctatatcg aattgctacg caatctagca gatgaagcag
    41281 gcttgccgaa aacgcttgat acagggagtt tagctggaat taaaacgcac gagtattgca
    41341 cgaataacca accaaacaac cactcagacc acgttgaccc ttatccttat cttgcaagtt
    41401 ggggcattag ccgtgaacag tttaagcaag acatcgaaaa cggcttgagc gctgcaacag
    41461 gctggcagaa aaatggcact ggctactggt acgtacactc agacggctct tatccaaaag
    41521 ataagtttga gaaaatcaac ggtacctggt attatttcga tggctcaggc tatatgcttt
    41581 cagaccgctg gaagaagcac acagacggca actggtactg gttcgacaac tcaggcgaaa
    41641 tggctacagg ctggaagaaa atcgctgaca agtggtacta ttttgatgta gaaggtgcca
    41701 tgaagacagg ctgggtcaag tacaaggaca cttggtacta cttagacgct aaagaaggtg
    41761 ccatggtatc aaatgccttt atccagtcag cggacggaac aggctggtac tacctcaaac
    41821 cagacggaac gatggcagat aaaccagagt tcacagtaga gccagatggc ttgattacag
    41881 taaaataaat agaaaggaaa ctttctaaat tgttctttca ccgcaggctc aggcttgcgg
    41941 ttttttattt gctctgattt ttcaaaaaag tgcttacttg aagaataggg aggtgttttg
    42001 tcaaaaataa aaacagtgac cgaaatcact gcttatcagt tgtagcaaat tcataaagtt
    42061 tttctgctgt gagaagtgcc attttgtcca tgcttgtttt tccttttctg aggtcagaaa
    42121 cagtagtcca tggaactcca gcaccttggg aaatagcaga tgtagacatc gaactgtcta
    42181 acaattcttg aataacttct ctcatcttat ttgtcctttt tattttttag ataaatatat
    42241 acattgattg caattataaa aatagctatt gcactaacca ttgcatttcc tctttccatt
    42301 tgataaaata gaggtgtaag gggctttcgc cccaacctct tagcgtttac ctttttcttt
    42361 gacgggattt cggtttacgc tttttgtttt gccttgcgac cgttattgcg gtcactagac
    42421 ttgcgatagc agttaccgtt tcaggaatat tgtctattgc cttttcaagt aacctgagcc
    42481 aatcttcttt gttcaacttc atcaccccct ttccttatct tgattatatt atatcacggt
    42541 aaaccgagaa agtcaagcgt tttgatgaag tttttttaat tttttcaaaa aaaaatagac
    42601 cttgtccaga ggtcggggag ttggaggggg caccctccaa aagcattgat ttaataaggt
    42661 tttattttac ctttttcata ataatctccc tatagagtca ccgcattcgg tggcttcttt
    42721 tgtgttggga ttcatgatat aataataaaa tcgataagta ggaaaagaga aaagagatgt
    42781 attatacgct tgaagaaaaa gaagtcttta tgagggaggc tttgagagag gctgagattg
    42841 ctcttgaaca cgatgaaatt ccaattggtt gtgtgattgt caaagatggg gaaatcattg
    42901 gtcgtgggca taatgcgcgt gaggaattac agcgagcggt tatgcatgcg gaaattatgg
    42961 ctatagagga tgcgaacttg agtgaggaga gctggcgctt gctggattgc acactttttg
    43021 tgaccattga accttgtgtc atgtgtagtg gggcgattgg gcttgcccgc attccaaatg
    43081 tggtctatgg ggctaaaaac cagaaatttg gcgctgctgg gagtttgtac gatatcttga
    43141 cagatgagcg tctcaatcat cgtgtggagg ttgaaatagg aattttggaa gatgaatgcg
    43201 cagctatcat gcaggatttt tttagaaata gacggaaaaa ataattttgc ttttaaaatg
    43261 aataggaatg tgatataata aatagtggag caacagttct gcgtgaagcg ggtcagggga
    43321 ggaatccagc agccctaagc gatttgaatt gtgtgctctt ttttcgtgca ctttaaaaac
    43381 cctttaaaat caacacttta aggggttttt gtttgtcttg tataagaaaa aggggcagac
    43441 gaggggcaca atttaaaatt ttatcttgtc taacttgcta gatatgtctg ataccatttt
    43501 ttgagtaacg tgagaataaa tctctagtgt ggtctttgag tcactatgcc ctactctgtc
    43561 catgatggca gtcaagggaa tgcccagctc agcaagtagg gatatgtgag aatgtctaaa
    43621 tgtatgtgta gttatgtttt tttctatgcc gattttttga ccatgtcttt tcaatgcaca
    43681 aataaccctg gcatttgtta ttggttctcc tagagtattg atgaaaataa aatctgtatc
    43741 aaatccattt gtcgcattct ctattatttg ctctttgata atatctaaca ctttttgagg
    43801 tgctgttata accctatcgg acttgattgt ctttggtgta gttctctctt tttgtctgaa
    43861 atcgtatgta tgcttgatgt gaatagtctt tttagaaaaa tctatatctt ccttgtagtt
    43921 taaggctgcc agctctccat acctcatgcc agtaagaaaa agaactttag ctattcggat
    43981 atactttgta attcgataat cacatagggc ctcgtctttt aaattttgga tgaataactt
    44041 aaactctttt tggtctaagt attttgtatt tttctttctg agttcgtcgg atgtaattac
    44101 ttttctaggc gtttcaacaa atagcatttc atttgtatca atataattca ttctgatagc
    44161 aaatttcatt atctgattga gcttgaattt gattttagaa acatagttat gagatctccc
    44221 atcttgtaat agttgatcta ttactttttg taataaacgt ctatcaaatt tctaactagg
    44281 tagtcgccct ctatctgctt taaaatctct ttttttacat tctttgaagc gtagacggtt
    44341 gagtttttta cgccgtgttt ccaattctcc tcaaattctt catatagttt ttcaaaagtt
    44401 atattagaaa cagaatgttg tttttctcct aacttttgtt ttatcttttc ctgaagtaaa
    44461 atcgcagctt gatttcttgc ttggggagtt tttttctcca tcgttactga gacttttttt
    44521 aatttctcag tatatggatc tttgtatctc tcaaaaaatt tatatttgcc gtttggcaat
    44581 tcttccatcc acattgattt tacctcactt ttttgataaa atgggtatag taaagagggc
    44641 tttttttatt atgagaattt cttgattact tctaaaattt cattggcata atttgcgact
    44701 tcgagagggg tagacaccgg gaagatgcct ttatcacgga tttcgatagt gctttttttt
    44761 gaatttgaac gataacgtaa tacccatttt ttgatgttat cgtcaaccaa aacattgaaa
    44821 tagctacggt tatctctgta aaatacacgt tctggagaaa ctacatccct agcaagcatt
    44881 ttaacaacag tataaacttc taattcagca ggagttgtga taatttcgtc agttacttcg
    44941 acaatctctt cagcttcagc ttcaactttt ggaatatcgg ttgttacttt ggtttcaacg
    45001 cttgtattaa gtgcagcact taatttttca ttaactcgct ctgtgatgaa ttgattaaac
    45061 cctttcacaa taatgggaga aaatgtcgtt aagatatttt gagtaacacg cccttcatag
    45121 atttcagaag ttagatatct gaggaagcta tctgacggtg tagtgatatt ttcagtaagg
    45181 aacgctttaa gattgttgag gtatttcaat tcagaagctg atgaaacaat attatcaata
    45241 tcaaaattct ctttgtggaa tttgatgatt tcagtaaatt gattttcttt gatatcagtc
    45301 acatcgattg ttaagaatgg agttgtgtcc attttatttg gctcatctaa atcagtaaag
    45361 aatttatatt ctctaccatt tgtcaagata ccgaattttg atttagtagt tacgaaatat
    45421 ctgaatagtt gagagtcgtg cttagtaagg ttttctgtaa ttgatttaca ttcaattagg
    45481 atttgaggct cgccatccaa aatgatcgca tagtcgactt tttcgccttt tttaatgccc
    45541 acgtcggcag taaattccgg gacaaattca agtggattga agatatcata tccaagcgct
    45601 tggaagaacg gcataatgaa ggcattcttt gtttgttctt cattcgtaat gctttggctg
    45661 aggtctgcta cacgtttgcc gacttgtttt aaatcggctt ttactttatc aatttccata
    45721 ttgatactcc tttttttaat ttactaatgc taaatactct tctttaacca tgatttcatt
    45781 tgtcatggtt tttagattgt agtaagacat gaatttgagg taatcaaact ctgtggggtc
    45841 gtctaagttt tctagagcgt cttttacgag atgatggatc atattcctat cagcttcgtt
    45901 ttcacagcgt agacgggcgt tctggtgctc tgagcgtgtg tggtctttgt gtccaagttc
    45961 gtgtagggcg acttggattt gttgttcagg agtcaagttg tggtcaatag cgagcacgtt
    46021 tgtgtctgga ttgtagaaac cgctattgtg ccagtttgaa ccgtcgaaga gacaaagttc
    46081 tacttgatat tcttcgcaga gtttagcgag tgtcatagtt ctccttcgtt atttatagac
    46141 atctctgcgg tgggcgattt ccacggctag gacgactagt ttatcgtctt ggatatcaca
    46201 gatgatgcgg taattctcta ctctgtatcg ccagtaacct gcaaggttgg cttttagtgc
    46261 ttttccgtgt tgtcgtggat tggtcgtgtt ttcaatgttt ttagcaagcc aggataggat
    46321 ttgttttctg gttggggtat ctagtttttt aagttgcttg agagcttttt tatcaatatc
    46381 tagccgatac attaagcaat atcctctcga gttagtccta gttcatccaa gacctcatcc
    46441 atggtataag tagctgggtc ggctaaatac tccatatagg cttggtcggc tgctcgtgcg
    46501 tcttcgatat cttccatgag tgccatgaag tcgtcaaaat ccatggtcgt tgtgtcgata
    46561 ccgtgtttgt ttaggtagtc cgtaatgtag gaattttttt ctgtgaagtt gatagtgata
    46621 gtcattagcg ttctcctttg cttttgaagt gggcggatag gacggatgta atgaagtcga
    46681 tatcatcttc atttagtggt ttcccgtcaa ataacatggt gttggctgct gctttgcgta
    46741 agtctatgat ttgtccgttt acttgggcaa attcatcact cccagcgata gcagggttat
    46801 ctgttcgtcc gagcaggtag tcggtggata cgttgaaata attagcaatt tctgcgatac
    46861 gttcagcgtt tggcgtagaa ttttttatct tatagagtgt atttctacca taacctaagt
    46921 cttcttctac ttgtccaaga gcttttccac gcttttttgc caattctttt attttttcaa
    46981 atgtctcaaa cattgttaaa tcaacctttc taagacatca caaaaaaatt taacaaattt
    47041 ggtgtaaaaa ggttgactaa ttatcccaaa aggtgtaaaa tgttttttgt aagtgataaa
    47101 caactaaaaa aacaactaag aaataaatta taaaaatgtt ttggcgaacg gtatttatag
    47161 atttattggt gtttttatta tgctttaatt ttagccgatt tggtgtaagt tgtcaagtat
    47221 aatgcagaaa aatagttaca tttttagttg tttcttattt acaaataagt aaagaggagg
    47281 aacgtgctga tggtaagtat tcttaaaaat ttagaacaag aaaaagacca ccttgaaaaa
    47341 gtcattaagg tagtcagcgc tggtggtaaa tttctgagat tgccatatca aaaaagtcac
    47401 gctcgattag tgagaatctg aaattgattt ctcaaaatct ttaccatcat cgtagatgtc
    47461 gcgtttgctt gctttgagta aatattcttt aaaagtcata aggttaactc ctttctgctt
    47521 atattatagc agaaaaagaa gaaagaaaaa gaaaggagaa aatatgccag atatcgcaaa
    47581 cggtcgcgaa agagttattg ctttcttgaa agagaaaggc attaaaaaag caactctagc
    47641 ggttgcttac ggctttaaac gacaggaagt gacaaacatt ctaagtggaa cgactaaagg
    47701 tccacgagcg aacagtttca ttcttcaggt tattgaagat tatgggattg agtaggaaag
    47761 atttgaggag taggaaaatg agaccaaaac gatatccgta tagtggacaa aaagagtcca
    47821 cctttgtaaa ggcagaccct gagttagttg aaaaactttt aagaaacact agttttcttg
    47881 agtgtttaca aaaaaagcct atcaattttc agatagactc agaagaattt aagcgtctta
    47941 gctatgaagc cattcatgat acttctcaag taactcaata ggaggaagga ataaaatgat
    48001 tcaccattat ataactcact atgccagcaa tgggaaagat tacgccgaag catggattca
    48061 aattgatttt ttgggaatgt gcttttgtgt atggaaaaag cgtacaacca ttgaacgatt
    48121 gtacgcaaac gaagattaga cttttttcca accgttgcct ttagcagatg tcggagggag
    48181 ccgatcacct tttccgatag ttgcggtatg accattagta acttttccgc cacgaggtcc
    48241 tacctctaca tagcgaccag gtttctgatt atctgttcca ggttttattg gagtatttgc
    48301 catactatct cctcctttct ataagtttga gaaaatcaac ggtatctggt attatttcga
    48361 tggctcaggc tatatgcttt cagaccgctg gaagaagcac acagacggta attggtacta
    48421 ctttgaccaa tcaggcgaaa tggccacagg ctggaagaaa atcgctgaca agtggtacta
    48481 ttttgatgta gaaggtgcca tgaagacagg ctgggtcaag tacaaggaca cttggtacta
    48541 cttagacgct aaagaaggcg ccatggtatc aaatgccttt atccagtcag cggacggaac
    48601 aggctggtac tacctcaaac cagacggaac actggcagac aagccagagt tcacagtaga
    48661 gccagatggc ttgattacag ttaaataaat agaaaggaaa ctttctaaat tgttctttca
    48721 ccgcaggctc aggcttgcgg ttttttattt gctctgattc ttcaaaaaag cgttttcttg
    48781 aagaataggg aggaggaatg gcagggtatt attgccaaaa acgccatttt gttaataata
    48841 gctccttttt attttttgat tattgtcaaa aacggtgttt tgtcaaaaat aaaaacagtg
    48901 accgaaatca ctgcttatca gttgtagcaa attcataaag tttttctgct gtgagaagtg
    48961 ccattttgtc catgcttgtt tttccttttc tgaggtcaga aacagtagtc catggaactc
    49021 cagcaccttg ggaaatagca gatgtagaca tcgaactgtc taacaattct tgaataactt
    49081 ctctcatctt atttgtcctt tttatttttt agataaatat atacattgac cgcaattata
    49141 aaaatagcta ttgcactaac cattgctttt cctcttttca tttgataaaa tagaggtgtg
    49201 aggggctttc gccccaacct cttagcgttc acctttttct ttgacgggat ttcggtttac
    49261 gctttttgtt ttgccttgcg accgttattg cagtcaccag acttgctata gcagttaccg
    49321 tttcagggat attatctatc gccttttcca gtaacctaag ccaatcttct ttgttcaact
    49381 tcctcacctc ctttccttat cttgattata ttatatcacg gtaaaccgtg aaagtcaagc
    49441 gatttgatga agtttttttg aaaaacaaag gggcaaaaaa ggggcaaaag tgtcgtaaac
    49501 ctctgtaaaa cgatgtaaaa aatcaacttt gccctcgctt taaagctcta aatttcaacg
    49561 tattgtgaaa cagtgtaaat tatcgtatcg cctataattg ttgtgtgctc tttttttcgt
    49621 gctttttccg aataaataag atagaataat ctagaataaa tgataataga aaagagaaaa
    49681 ttatgaaaat tcgtggtttt gaattggttt cgagttttac agatgaaaat ttattgccca
    49741 agcgtgagac agcgcatgcg gctggttacg acttaaaggt tgctgtgcgt acagttgttg
    49801 cgccaggaga gattgtcttg gttccgacag gggttaaggc ttatatgcag ccgactgagg
    49861 ttctctacct ctatgatcgt tcttcaaatc ctcgtaagaa gggcttggtt ttaattaact
    49921 cagttggggt cattgatggg gattattatg gaaatcctgg aaatgaaggg catatttttg
    49981 cgcagatgaa gaatatcaca gatcaagagg ttgttcttga agttggggaa cgtattgtcc
    50041 aggctgtttt tgctactttc ttaattgcag atggagatgc agctgatggc gttcgaactg
    50101 gtggatttgg atcgacaggg cactagaatg aagattatct ttgtacgtca tggggagcca
    50161 gattaccgtg agttagagga gcgttcttat ataggatttg ggatagattt ggcacccttg
    50221 tctgagatgg gacggcagca agtccagaaa ttgagcaaaa atcctttact ctcgtcagct
    50281 gaaataatcg tatcttctgc agtcacaaga gctttagaaa cggcttcgta tgtggtctgt
    50341 gctacgggtc ttcctttaag agtagagcct ttattacatg aatggcaggt ctataaaaca
    50401 ggaatagaaa actttgaaac agctagaaga ctgtttttag aaaacaaggg ggagttgctt
    50461 cctaatagtc ctattcaata tgagacagct acggaaatga agtctcggtt tctagaatgt
    50521 atgtctaagt atcgagaaca tcagactgtg gtagttgttg ctcatcgaat gctcatgcgc
    50581 cagtttgtgc caaatgagaa gattgatttt tgccaagtga ttgagtgtga gttagagata
    50641 tagaaagagg tttgtcatcg caaagaaaaa agcgacattt gtatgtcaaa attgtgggta
    50701 taattcccct aaatatctgg gacgttgccc caactgtggg tcttggtctt cttttgtgga
    50761 agaggttgag gttgccgagg tcaagaatgc gcgtgtgtcc ttgacaggtg agaaaaccaa
    50821 gcccatgaaa ctagctgagg tgacttccat caatgtcaat cgaaccaaga cggagatgga
    50881 ggaattcaac cgtgtgcttg gaggcggagt ggtaccagga agtctcgtcc tcatcggtgg
    50941 ggatcctggg attgggaaat caactcttct cctacaagtc tcaacccagt tgtcccaagt
    51001 ggggacagtt ctctatgtca gtggggagga gtctgcccag cagattaaac tacgtgcaga
    51061 gcgcttaggt gatattgata gtgagtttta tctctatgca gagaccaata tgcagagtgt
    51121 tcgtgcagaa gtggagcgta tccagccaga ctttctcatt attgattcca tccagaccat
    51181 catgtctcct gagatttcag gggtgcaggg gtctgtttct caggtacgtg aagtgaccgc
    51241 tgaactcatg cagttggcca agaccaataa cattgccatc tttatcgtag gtcatgtgac
    51301 caaagaagga accttggctg ggcctcgtat gttggagcat atggtggata cggtgcttta
    51361 ctttgaaggg gagcgtcacc acacctttcg tattttgaga gcggtcaaaa atcgttttgg
    51421 ttccactaat gagattggga tttttgagat gcagtcgggc ggcttggttg aggtactcaa
    51481 tccgagtcaa gttttcctag aagagcgttt ggatggggcg actggttcct ccatcgttgt
    51541 aaccatggaa gggacgcgtc cgattttggc ggaggttcag gctttggtaa caccgaccat
    51601 gtttggaaat gccaagcgta ctacgacagg acttgatttt aaccgtgcta gcttgattat
    51661 ggctgttttg gaaaaacggg cagggcttct cttgcaaaat caggatgcct atctcaaatc
    51721 tgctggtggt gttaaattgg atgaacctgc cattgacttg gctgttgcag ttgctattgc
    51781 ttcgagctac aaagacaagc caactaatcc tcaggaatgt tttgtcggag aactgggctt
    51841 gacaggagag attcggcgcg tgaatcgtat tgagcaacgc atcaacgaag ctgctaaact
    51901 gggctttact aagatttatg tacctcagaa ttccttgaca ggaatcactc tgcctaagga
    51961 aattcaggtc attggcgtga caacgattca ggaagtcttg aaaaaggtct ttgcataatc
    52021 cgtgacaaat tctcttaaaa atgataagat aggagaaata tttgactatc aaattttcaa
    52081 ggagggaatc gtgtcgtatt ttgaacagtt tatgcaagcc aatcaggctt atgttgccct
    52141 acatgggcag ttaaatctgc cacttaaacc taaaaccaga gtagccattg tgacctgtat
    52201 ggactcacgt ttgcacgttg cgcaagctct gggcttggca cttggggatg ctcatatctt
    52261 gcggaatgca ggtggtcgag tgactgaaga catgattcgt tcgctagtta tttcccagca
    52321 acaaatgggg acaagagaga ttgtggtatt gcaccataca gactgtggtg ctcagacctt
    52381 tgaaaatgaa ccttttcagg agtatttaaa agaggaatta ggtgtagatg tgtcagacca
    52441 ggacttcttg cccttccaag atatagaaga gagtgtacgc gaggatatgc aactgcttat
    52501 cgagtctccc ctaataccag acgatgtcat tatctctggt gctatttaca atgttgatac
    52561 aggaagtatg acagtcgtag aattataaat acttcattta gaaagaaagt gtatgaagaa
    52621 aagcagtatt ttatttattt ttattttatt gctatgtatt ggtttacagt atgaaaccat
    52681 ctactatacg gacggttcga ggtcaggtgc ggaatatgga ctaatgggag tttctatctt
    52741 tctagctctc ttttacatga ttccggctct ttattttctc ttccgtattg ggaaaaaatg
    52801 ggaattgcca aagaaggttt tgattctgtc tttattggga gggatgttcc tttcaggctg
    52861 gttgtctagt tttgctaata cttatataca tgatttactg ggggttcttt tcccagatag
    52921 tccattttta aatgcctttg aaagtgctat tgtggctcct ttggtagaag aacccttgaa
    52981 attattgcca cttgtttttg ttttggcttt gattcctgtg cgaaaattaa aatttttgtt
    53041 tttacttgga attgcctccg gtttgggatt ccaaatgatt gaggatattg gttatattcg
    53101 tacggatttg ccagagggct ttgactttac tatttcgcga attttagagc gtatcatctc
    53161 aggaattgcc tctcactgga ctttttcagg tctagctgta gtaggtgttt acttgcttta
    53221 cagagcctat aaaggacaga aggttggcaa gaaacagggt cttattgttt aactctcctt
    53281 ttgtggagtt ggaaacagag ttgcctttag cgattccagt ggttacggct attgctctct
    53341 atggttatta tcatgcttat tgctttgttg agaaacacaa tgagttgatg acctagaata
    53401 tttttcaaaa gaatgatgca agggtacaaa tatggtgccc ttcttttatt tttgattgaa
    53461 aaatagtgca aaaagcgcta caatggtaga tggaaaatct tgtgaaaagc acaagcgata
    53521 catatatacc ggaggaaatc atgtcttttt ctgatttaaa gctgtttgcc ctttcttcta
    53581 ataaagaatt ggcagaacgt gtggcgcagg agattgggat agagttgggg aaatcaagtg
    53641 ttcgccaatt ttcagatgga gagattcagg tcaacattga agaatcaatc cgtgggaaac
    53701 acgtctttat cttacaatca actagttcgc ctgtaaatga caatctgctt gaaattttga
    53761 ttatggtaga tgctttgaag cgtgcgagtg cagagtctgt caatgttgtc atgccttact
    53821 atgggtatgc acgtcaggat agaaaggcga gagcgcgtga gccaatcact tcaaaacttg
    53881 tcgcaaatat gcttgaagta gctggagtgg atcgtttatt gaccatcgac ttgcatgctg
    53941 cgcaaattca aggattcttt gatattcctg tggatcattt gatgggtgct cctctaattg
    54001 cagattattt tgagcggcgt ggtatggttg gttctgacta tgtggttgtc agcccggacc
    54061 atggaggggt gactcgtgct cgtaagttgg cagaattttt gaaaacatct atcgctatta
    54121 ttgataaacg tcgtagcgtt gataagatga atactagtga agttatgaac atcatcggta
    54181 aggttgaagg caagacttgt atcttgattg atgatatgat tgataccgct ggaacgattt
    54241 gtcatgcggc agatgctctt gcggaagctg gtgctgttgc agtctatgca agctgtacgc
    54301 acccagttct ttctggtcct gctacggaca atatccaaaa atcagctatt aagaaattgg
    54361 ttgttttgga taccatctat ctgccagaag agcgtttgat tgataagatt gagcagattt
    54421 caatcgctca tctcctaggg gatgctatcg tacgtattca tgaaaaacga ccactttctc
    54481 cacttttcga tattgagaaa aaaatttaat gaccaagcct gagatgattc tcaggttttt
    54541 tcgtcttttt tccgaataaa tggatagtag cggtgaatct agtaaagcta gatttaaaac
    54601 tgtgatataa taaaaagaga agaaatatga ctatacgtca tcagaagttt cagcgaccat
    54661 catttttgaa cagtgatagc acttgaatcg acgctttcta aggagaattc tagtaggtat
    54721 accagtcgtt tcaagataag gaattttaga aggttt
//
LOCUS       DAVKFH010000009        48735 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKFH010000009 DAVKFH010000000
VERSION     DAVKFH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.009"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(47..1936)
                     /locus_tag="VW006_000551"
     CDS             complement(47..1936)
                     /locus_tag="VW006_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9379451.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNFFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2074..3639
                     /locus_tag="VW006_000552"
     CDS             2074..3639
                     /locus_tag="VW006_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9379452.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3639..4379
                     /locus_tag="VW006_000553"
     CDS             3639..4379
                     /locus_tag="VW006_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9379453.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4488..4703)
                     /locus_tag="VW006_000554"
     CDS             complement(4488..4703)
                     /locus_tag="VW006_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379454.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VW006_000555"
     CDS             complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VW006_000555"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9379455.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6874..7230)
                     /locus_tag="VW006_000556"
     CDS             complement(6874..7230)
                     /locus_tag="VW006_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9379456.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7468..7704)
                     /locus_tag="VW006_000557"
     CDS             complement(7468..7704)
                     /locus_tag="VW006_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379457.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VW006_000558"
     CDS             complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VW006_000558"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9379458.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VW006_000559"
     CDS             complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VW006_000559"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9379459.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9368..11068)
                     /locus_tag="VW006_000560"
     CDS             complement(9368..11068)
                     /locus_tag="VW006_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9379460.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11268..12935)
                     /locus_tag="VW006_000561"
     CDS             complement(11268..12935)
                     /locus_tag="VW006_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9379461.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12938..13303)
                     /locus_tag="VW006_000562"
     CDS             complement(12938..13303)
                     /locus_tag="VW006_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9379462.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13460..13648)
                     /locus_tag="VW006_000563"
     CDS             complement(13460..13648)
                     /locus_tag="VW006_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9379463.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13764..14249)
                     /locus_tag="VW006_000564"
     CDS             complement(13764..14249)
                     /locus_tag="VW006_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9379464.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            complement(14518..16062)
                     /locus_tag="VW006_000565"
     CDS             complement(14518..16062)
                     /locus_tag="VW006_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9379465.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16275..16577
                     /gene="gatC"
                     /locus_tag="VW006_000566"
     CDS             16275..16577
                     /gene="gatC"
                     /locus_tag="VW006_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9379466.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16577..18043
                     /gene="gatA"
                     /locus_tag="VW006_000567"
     CDS             16577..18043
                     /gene="gatA"
                     /locus_tag="VW006_000567"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9379467.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18043..19485
                     /gene="gatB"
                     /locus_tag="VW006_000568"
     CDS             18043..19485
                     /gene="gatB"
                     /locus_tag="VW006_000568"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9379468.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19614..20174
                     /gene="efp"
                     /locus_tag="VW006_000569"
     CDS             19614..20174
                     /gene="efp"
                     /locus_tag="VW006_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9379469.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20196..20585
                     /locus_tag="VW006_000570"
     CDS             20196..20585
                     /locus_tag="VW006_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9379470.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20578..21000
                     /gene="nusB"
                     /locus_tag="VW006_000571"
     CDS             20578..21000
                     /gene="nusB"
                     /locus_tag="VW006_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9379471.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     AVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21049..21276)
                     /locus_tag="VW006_000572"
     CDS             complement(21049..21276)
                     /locus_tag="VW006_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9379472.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(21767..21952)
                     /gene="briC"
                     /locus_tag="VW006_000573"
     CDS             complement(21767..21952)
                     /gene="briC"
                     /locus_tag="VW006_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9379473.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22111..22878)
                     /locus_tag="VW006_000574"
     CDS             complement(22111..22878)
                     /locus_tag="VW006_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9379474.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22875..23741)
                     /locus_tag="VW006_000575"
     CDS             complement(22875..23741)
                     /locus_tag="VW006_000575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9379475.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23778..25145)
                     /gene="accC"
                     /locus_tag="VW006_000576"
     CDS             complement(23778..25145)
                     /gene="accC"
                     /locus_tag="VW006_000576"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9379476.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASRNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25157..25579)
                     /gene="fabZ"
                     /locus_tag="VW006_000577"
     CDS             complement(25157..25579)
                     /gene="fabZ"
                     /locus_tag="VW006_000577"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9379477.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(25576..26061)
                     /locus_tag="VW006_000578"
     CDS             complement(25576..26061)
                     /locus_tag="VW006_000578"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9379478.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26064..27299)
                     /gene="fabF"
                     /locus_tag="VW006_000579"
     CDS             complement(26064..27299)
                     /gene="fabF"
                     /locus_tag="VW006_000579"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9379479.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27321..28052)
                     /gene="fabG"
                     /locus_tag="VW006_000580"
     CDS             complement(27321..28052)
                     /gene="fabG"
                     /locus_tag="VW006_000580"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9379480.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28086..29006)
                     /gene="fabD"
                     /locus_tag="VW006_000581"
     CDS             complement(28086..29006)
                     /gene="fabD"
                     /locus_tag="VW006_000581"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9379481.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTSLLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTTHLAHVEDQASLVALLKK"
     gene            complement(28999..29973)
                     /gene="fabK"
                     /locus_tag="VW006_000582"
     CDS             complement(28999..29973)
                     /gene="fabK"
                     /locus_tag="VW006_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9379482.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(30092..30316)
                     /locus_tag="VW006_000583"
     CDS             complement(30092..30316)
                     /locus_tag="VW006_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9379483.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30376..31350)
                     /locus_tag="VW006_000584"
     CDS             complement(30376..31350)
                     /locus_tag="VW006_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9379484.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31350..31784)
                     /locus_tag="VW006_000585"
     CDS             complement(31350..31784)
                     /locus_tag="VW006_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9379485.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31878..32663)
                     /locus_tag="VW006_000586"
     CDS             complement(31878..32663)
                     /locus_tag="VW006_000586"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9379486.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33032..34396
                     /locus_tag="VW006_000587"
     CDS             33032..34396
                     /locus_tag="VW006_000587"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9379487.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34399..34770
                     /locus_tag="VW006_000588"
     CDS             34399..34770
                     /locus_tag="VW006_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9379488.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            34984..36258
                     /gene="serS"
                     /locus_tag="VW006_000589"
     CDS             34984..36258
                     /gene="serS"
                     /locus_tag="VW006_000589"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9379489.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVEAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36283..37182)
                     /locus_tag="VW006_000590"
     CDS             complement(36283..37182)
                     /locus_tag="VW006_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9379490.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37292..37840)
                     /locus_tag="VW006_000591"
     CDS             complement(37292..37840)
                     /locus_tag="VW006_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9379491.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38058..39380)
                     /locus_tag="VW006_000592"
     CDS             complement(38058..39380)
                     /locus_tag="VW006_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9379492.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFTFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39471..39559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39723..42059)
                     /locus_tag="VW006_000593"
     CDS             complement(39723..42059)
                     /locus_tag="VW006_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9379493.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKGQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQIGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42163..42711)
                     /locus_tag="VW006_000594"
     CDS             complement(42163..42711)
                     /locus_tag="VW006_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9379494.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42708..43010)
                     /gene="zapA"
                     /locus_tag="VW006_000595"
     CDS             complement(42708..43010)
                     /gene="zapA"
                     /locus_tag="VW006_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9379495.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43097..43978
                     /locus_tag="VW006_000596"
     CDS             43097..43978
                     /locus_tag="VW006_000596"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9379496.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            43982..44596
                     /gene="lepB"
                     /locus_tag="VW006_000597"
     CDS             43982..44596
                     /gene="lepB"
                     /locus_tag="VW006_000597"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9379497.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            44830..47196
                     /locus_tag="VW006_000598"
     CDS             44830..47196
                     /locus_tag="VW006_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9379498.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKNNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47244..48527)
                     /locus_tag="VW006_000599"
     CDS             complement(47244..48527)
                     /locus_tag="VW006_000599"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9379499.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        14273 a        11187 c         9193 g        14082 t
ORIGIN      
        1 tctatcttcc attatatcat actttttcag tctatggtag ctctctttaa aaggctccga
       61 tactgccgcc acctccgcct ccagagaagc caccgccaga acttccactt ccagaagata
      121 cggagtaggt acttgctgta tttgcgacac tagcataatg gctcatttgc gcgcttgaat
      181 gataaaacat actgtgccag ccataagcta catagagatt gatatctgga ttttcaactt
      241 gaatctgatg aaccttcatc aaatgactaa ccttgtccgc atagccaaat aaggttgcat
      301 agaccaagag acgattccaa acaacaatac tttccaattc agccttatcc agtcgtgcaa
      361 tctcacgtaa catattttca aaactggtcc agagatagta gacctcagct cccgcttcat
      421 ttagaacacc atcacgatta tctagtcgaa gcttccaata atagaaaaca gacaaaacta
      481 accctagaaa accaagtatt ggcaaaggga ggtaaagata gccatgaacg tctaaactgt
      541 acaagaacaa accaaatccg ataaataggg gcaagatagt caagacaccc atacccactt
      601 gcaaagtctt ttccaaacca gttaaaggac gatagtaatc tgggagcccc cagaaggaaa
      661 ctcgttttct cactccttct tgcatctggt tcaatacctc ttcaaaagaa gatttgagtt
      721 gaagccctct tgcttgaatc cgtttttcat cagaaacttt ggctctacga taaagactat
      781 cagatacctt gtaatccgca aaaaaattgg aaagagtggc ttcttttttg cctgaaaagg
      841 ctagatttag acagtctctc tcaaatcttg acaaaccatc ttctttcacc aatctcaagc
      901 caactgcatc tccttctgaa ataatagaga cattcccacg gtctatcaca tctagcaagg
      961 tagcttgaat aagttggtca aaagtgaatt tgccagcccc cttgaccaag ggactcactt
     1021 cctccaagga ggtcgagtag actgcttctg ataaaaccat aggctctaat tccattggtg
     1081 gttcatagag acgatgattt ttggcatatt tgactgaagg agtggtcttt cttctataaa
     1141 taaaatagaa gcagacactc aataacaagg agatagaaag tatcgaaggg aggacccaag
     1201 taacgagttg tttactctga tctttttctc taacaatcga gtcttctatc ttattaaact
     1261 cttctaaacg attccctttc aagccctgat ccctagcgct agcaaaatcg gtccgagacc
     1321 aataggcatg caactcaact ccacgcttag ccggaagatt gtctaaacgg atagtataat
     1381 caaggttact cttttcaatc gttccctctc taaaaagttt ccctgtatgg aaaaagagtt
     1441 tctcagcccc cttgtctccc cttacatgaa attcaaactt ttcaatagac tctgaactat
     1501 ctgtcagagg ttgccaattt aattcagcga tatcatcata aaggaaaagt aaatttttta
     1561 agttccagac gaggtcaact tcaactatgt cgccctcctg acctggatta tagactctca
     1621 cagtataacc atccgcttct tctgttactt cgctagtcac atctgctagt tctgcaccgt
     1681 ttttcgcagc ctgaaccttt ggatgagggt caatgtcaaa cccgctaggc atcttaccag
     1741 cacgtccaag tcccacgatt tggcccttaa agtcctcctc aaactggtaa actatcttct
     1801 gtctaaactc tgccgtatta tctgcatgaa tatacaaatc cccttgataa gagtttatct
     1861 tgaaatcaat ggcaaaaaca gagagtggaa gaaggcaaaa caagcctaac accagtaaga
     1921 aaaaagtttt tttcatcaac taagccccct ttttatcttt cttatcatta tagcatcttt
     1981 tcgtaagtaa aggcttactt atgttgaaaa attacgcatt tttcgataaa ataggagaca
     2041 gttatttttt aatagatcag aataggagaa atcatgagaa aaatatacct atctattttc
     2101 acaagtctct tgctgatgct aggacttgtc aatgttgctc aagccgatga atatttacgc
     2161 atcgggatgg aagcagcata tgctcccttt aactggaccc aggatgatga tagcaacgga
     2221 gctgtcaaaa tcgatgggac caatcagtat gccaacggat acgatgttca aatcgccaag
     2281 aaaatcgcta aggacttagg taaagaacct ttggttgtta aaaccaagtg ggaaggtcta
     2341 gtccctgccc ttacttctgg taagattgac atgattatcg caggtatgag tccaactgca
     2401 gaacgcaaac aagaaattgc cttttcgagc agttactaca ctagcgaacc agttttgctt
     2461 gtcaaaaaag attctgccta cgcaagtgct aaatctttgg atgactttaa tggtgcgaaa
     2521 atcacttctc aacaaggtgt ctacctctac gacttgattg cacaaatccc aggtgctaaa
     2581 aaagaaacag ccatgggaga cttcgctcaa atgcgccaag ctcttgaggc tggtgtcatt
     2641 gatgcttatg tttctgaacg tccagaagca ctgactgctg aagctgcgaa ctctaagttc
     2701 aagatgattc aagtagaacc tggtttcaaa actggggaag aagatacagc tatcgctatc
     2761 gggcttcgta aaaatgacaa tcgtattagc caaatcaatg ccagcattga aaccatttca
     2821 aaagatgacc aagttgcctt gatggatcgt atgatcaagg aacaacctgc cgaagctaca
     2881 acaactgaag agactagcag tagtttcttt agccaagtcg ctaaaattct ttctgaaaac
     2941 tggcaacaac tcttgcgtgg tgctggtatc actcttttaa tctctatcgt cggaaccatc
     3001 ataggtctca ttattggact tgccattggt gtcttccgta ctgctcctct ctctgaaaac
     3061 aaagccattt atggccttca aaaactagtc ggctgggttc tcaatgtcta cattgaaatt
     3121 ttccgtggta cgccaatgat tgttcaatcg atggttatct actatggaac tgcccaagct
     3181 ttcgggatca accttgaccg tacactggct gctatcttca tcgtttcaat caataccggt
     3241 gcctacatga ctgaaatcgt ccgtggtggt atcctagcag ttgacaaggg acaatttgaa
     3301 gctgcgactg ctcttggtat gacccataac cagaccatgc gtaagattgt cctacctcag
     3361 gtagtccgta acatcctacc tgcaactggt aatgaatttg tcatcaatat caaagataca
     3421 tctgtattga acgttatctc tgttgtcgaa ctttatttct caggaaatac cgtggcaaca
     3481 caaacctatc aatacttcca gacatttaca atcatcgccg tgatttactt tgtcctcacc
     3541 ttcaccgtaa cacgtatcct acgatttatc gagcgccgaa tggacatgga tacctatact
     3601 acaggtgcta accaaatgca aacggaggat ttgaaataat gacacaagca atccttgaaa
     3661 ttaaacacct caaaaaatcc tatggacaaa acgaagtgct aaaagacatt tcactcactg
     3721 tccacaaggg agaggtcatc tctatcatcg gaagctctgg aagcggaaaa tcgaccttcc
     3781 tacgctccat taacctactt gaaacaccaa ctgatggaca aatcctttat catggacaaa
     3841 acgtcctcga aaaaggctat gacctcacgc aataccgtga aaagttgggg atggttttcc
     3901 aatcctttaa cctctttgaa aatctcaatg ttcttgaaaa cacaatcgtc gctcagacaa
     3961 ctgtcctaaa acgcgaacgc acagaagctg aaaagattgc caaagaaaac ctggaaaagg
     4021 tcggcatggg agaacgctac tggcaagcca aaccaaaaca actctcaggt ggtcaaaaac
     4081 aacgtgtggc catcgctcgt gccctctcca tgaatccgga cgctattctc tttgatgaac
     4141 caacatcagc tctcgatcca gaaatggttg gagaagtcct caaaatcatg caggacctgg
     4201 ctcaggaagg cttgaccatg attgtcgtaa cccatgaaat ggaatttgcc cgtgatgtct
     4261 ctcaccgtgt tatctttatg gataagggcg tgatcgctga agaaggtaaa ccagaagacc
     4321 tcttcaccaa tcctaaagaa gaccgaacaa aagagttcct tcaacgctat ctcaaataaa
     4381 aagaaaaggc tgcatcaacc gtgcagtctt tttgctgtcc tcaaaatgaa aaggcagatt
     4441 ctctatctct actacttgaa ggtcaagaat ccgccgttat ccaaagatta atcttcaaat
     4501 ttttcataat ttttttcata gaaatcaatt aagcctaggg ctgtttcaaa tgaaatattt
     4561 tttacttttg cacgaccctg cgccagagca atgatagaca tttcacgcgc attcgtttct
     4621 ttagagatac ggtagcctgt gattttctta tcacgaaccc agccaacaac tgattctact
     4681 ttttcaaagt ttgacttagc catgtttttc tcctattttc ttctttataa tatttaattg
     4741 tatacgtttt tactactttt gtcaataaaa aaaacatata ttcaacgaaa ataattagtt
     4801 cagaattttc ttacttcctt tttaaatccg ataatatata ggttttgcta gcctctacac
     4861 cataaatatt tgctaataaa actactgtat tattaccctc ttaaggtgcg aaattattca
     4921 agttcttaaa aaatgcttct ctatttccgt aagatagctt atttcttcga tttaagaact
     4981 gaagatgata gttctaacct ttagaaatcc aagccttcac tagctcaaaa actcctatct
     5041 ttcttcctca ataaatcatc tgagagatag gtatttctta tatccttcaa attcatcact
     5101 cctttggata ttgaggtgct agcctagaag cagacacccg attatctaaa ccatccttaa
     5161 tagagatgac aagacagata agctcagtta attacttata tatcttttta atatggtata
     5221 tttgtcaaca aaaagaaatc aaatcatacc ttatactcaa tgaaaatcaa agagcaaact
     5281 aggaagctag ccgcaagttg ctcaaaacac tgttttgagg ttgcagatag agctaacgtg
     5341 gtttgaagag attttcgaag agtattaaag acagtgtgac tatgcttgtc aatagatagg
     5401 aaatcaaaat gaaggatgga aatctgagcc aaacatcata agtgctataa tgaacagaag
     5461 taagaattgc acgataagtt ttctaaaacc ataggaatcg tcccctttct tcatcttcat
     5521 tatagcatat atacatcagt tgtacaaata atatgatatt tttttattag tcctcagaca
     5581 agcatattat aaagcgtttc attaccattt aagttaatat aagctggatc aaaaccttcc
     5641 attctacgaa tcaaacctgc ataatcatgc ttatctgcta aagcgatccc aatcaatact
     5701 gggcctgttc ccttgctagc tcgtttgata tactcaaaac gtgtgatatc atcatttggc
     5761 cccaggatat catttacaaa ctcacgcaaa gctcctggac gttgtgggaa attgaccaca
     5821 aaataatgtt tgataccatc ataaatcaag gcacgctctt ccatttctgg catacggttg
     5881 atatcattat ttcctccaga aatgatacaa caaatggttt tccccttaat atattcagct
     5941 aaaacctcta aagaggcgat actagctgct ccagcaggtt ctgcgactat cccttgctta
     6001 gagtaaaggt caatcaaggt ttcagaaatc aatccctcat cgacacctac caaagtttga
     6061 atatgttgac gagttgcttc ataggtcaac tgacctacct tttgcacagc aatcccatcc
     6121 gcaaatttat caatttcctt gagtttgact ggacctccag cctcaaaggc agctttcatg
     6181 gaacgcgctc cattcgcctc tactccgata acctcaatct ctggacttgt ttccttgata
     6241 taggtagaaa ccccggcaat gagaccgcca ccaccaacag gaaccaagac agcatcaaaa
     6301 tcaatcgatt cttttcgagc ttcttctaaa atctcataag caactgttcc ttgacctgct
     6361 tgaacatgag catcatcaaa aggatcaata aaggtacgat tttcagagac tgtaaattct
     6421 tgagctgctt tggctgaggc atcaaaggta tctccaacta gtttaatagt tacaaaatcc
     6481 ccaccaaaaa agcgaacctg accaatcttt tgttgtggcg tagtaatggg cataaagata
     6541 gtagcaggaa ttttcatttc attacaagta taggctactc cctgcgcatg atttcccgca
     6601 gaagcgcaaa ctaccccacg ttcacgttct tccttgctga gctgggaaat ggcataatag
     6661 gcaccacgaa ttttaaagga gcgaacacgc tgggcatttt cttttttcaa ataaatctta
     6721 gcaccatact tctccgataa ataatgatcg taatccagtg gagtattcac aaccacaccg
     6781 ttcaagacct tgtgagcctt gattatatct tttgaactta acatataaaa cctctttttc
     6841 tataaaaaac agtcactaat gactgttttt tagttattca tctccgaaaa tttcagtaac
     6901 actttcatgg tatttatgag ccatacgaat cgaaaactct ttaggaagtt cataacgatt
     6961 tgtggaaatc caatcatata accattctac caaactttct tcactatcat tacgatggaa
     7021 gataaaatag tttaaaattt ctttatattc tacatcattc aaatccaacg tgtctacaaa
     7081 ttgactacga ggaacaaaat tagaatctaa ttttcttagt tgatctgtca attctcccaa
     7141 ttgtaaatcc aactgaaaaa atagtttttg cagttgccta tcagtttcat cacatcctgt
     7201 cttgatagaa aacaaattgg taatattcat atgatttcaa cccctaaata agtacatcta
     7261 atatcccacg aatagctgaa gctaaagttt tcgcagtcca gtcatttaaa cccgattctt
     7321 tcaatccaga tgcaataatt tgctctattt gatataaacc gccatctatc gtctcattta
     7381 ttctgcgaag aggaccttca atataggata aagcttctcc gaatctaaca gctgatttag
     7441 gtcctaatga tttaccaact atatttgtta atatttttta tttctcaaaa tccatttcgc
     7501 tatataacga gcagatttag tcgcaccagc tctctcaaac ggagaatgaa tttcaagacc
     7561 ttcttctcta gctatttgtg ccaatttgaa gaattgttca aaggtcatat gcaacctacc
     7621 atcttcaaaa ttagcatcat cataaatgtt aattacagca ttttcatcat acgaccaatc
     7681 tgctttattt tggtctgcta gcactattac cgacggtgta attacattat acaactagaa
     7741 ccaataataa cgatgcaatt tttctcataa tctttttcat aaaatcatct ccagcgctat
     7801 atatttctaa ttagttatag attttgaatg catcatcgtc gtttttacca acgaatggca
     7861 ttgctttacg caattctgca ccaacttttt caatttcaag gttagctgct tgttcacggt
     7921 aagcagtcaa ttttggacgt ccagctttat agtcatttac aaagtcattt gcaaatttac
     7981 cattttggat gtctgccaag acagccttca tattttcttt aacttgttca gtgattacac
     8041 gtggacctga tacatagtca ccgtattcag cagtgtttga aatagattga cgcattttct
     8101 tgaatccacc ttcgtagatc aagtcaacga tcaatttcat ttcgtgaaga acttcaaagt
     8161 aagccaattc tggagcgtaa cctgcttctg tcaagacttc gaaacctgct tcgataaggg
     8221 cagtcaaacc accacaaagt acagcttgtt caccaaacaa atcttcttca gtttcttctt
     8281 tgtaagttgt ttcaagaaga cctacacgag ccgctccaac acctttacac cagtccatag
     8341 caatgttttt agcatttcct gttgcatctt ggtatactgc ataaagagct ggaacaccaa
     8401 atccttcttc gtaagtacga cgtaccaagt gtcctggtcc tttaggagca cacatgaaga
     8461 catctacatc cgcaggaact ttgataaatt caaagtggat gttgaaacca tgggcaaatc
     8521 caactgcgtt tccagcttcc aagtttggag cgatttctgc ttcgtacaat tcttgttgaa
     8581 tttcgtctgg cgccaagatc atgataacat cagccaactt agtagcttct gctactgtgt
     8641 aagtatcaaa tccatcttct tttgctttat caaaagattt acctggacgt acaccgataa
     8701 taacgtcacg acctgaatca cgcaagtttt gagcatgcgc atgcccttgt gaaccataac
     8761 cgataacggc gattttttta ccgtcaagtg ctgctacttt aacatctttt tcatattcca
     8821 tttgaactgt catagctttt ctctcttttc tattatttat tgccttttaa gctggtttaa
     8881 caaatttaag ttggattttt aatcgcgggt aaatccagtt gcacccgttc gagcaatatt
     8941 gcgaataccg tatgggcgaa tgactcgcaa tagggcttcg ctcttttctg catttcccgt
     9001 catctgaatg gtaatcgagc ttggcgctac gtctaccact gttgcacgga aaggttgaat
     9061 aatcgctaaa atctcagctc tcttctcagc tggcgctgac atcttaacca aaatcacctc
     9121 gcgctccaaa tgaggcttgt ctgtaatatc tcgaatgcga atcacatcaa tctgacgatt
     9181 gagctgtttg atgatttgct ccacttcatc atgagaagca acatcaataa taatagtgat
     9241 acgcgataca ttcggatctt ctgttgctcc aacagagatg ctttcaatat taacctgacg
     9301 acgagatagg acacctgtaa agcgattgag gactcctgaa cgattttgta gttttgctgt
     9361 taacattcta cgcatggaac tgcaccccca acatctcatg attactctta ccagccggta
     9421 ccattggtaa cacctgttcc ttacgagaaa tatctacctc aattagcata ggaacatcct
     9481 cagtgatgac ttcaaggtct tgagccaagg tctcaggatt gtcaaactta tagtttttaa
     9541 taccataagc ctgcgccatc aattggaaat caggaagggt atcaaagacc gactctgatg
     9601 ttctgccttc atagaaggat tcctgccact ggcgaaccat tccgagtgaa tgattgttca
     9661 gcataaccac cttgattggc accttgtaaa tattcaaaat agccaactcc tggttggtca
     9721 tttggaaacc accatcccca acaaacaaga ctacttcctt atctgggtta gcaattttag
     9781 caccgattgc tgctggaatt ccaaagccca ttgttcccaa accacctgaa gtcactaact
     9841 gacgttcatt ttggtaggga taatactgag ctgtccacat ttggtgttga ccaacgtctg
     9901 ttaccacaat ggcatctcca ttcgtcaatt caccaattcg ttcaataact gcttgcggtt
     9961 gaaccacacg ctctttcttg tcatacgaac gaacacgatt cttgtcttta gtgactttct
    10021 caatccactt ttcagtattg ttgtgaactg ttggttctgc tagcaacatt tgcaaggcct
    10081 tcttagcatc tccaactaca ggaatgtctg cactgataat cttgccaatc tcagctgggt
    10141 caatatcaat gtgggcaacc ttagcattct tagcgaaagt cttaggattc cccgtcaaac
    10201 ggtcatcgaa acgagaacca atactaatca taaagtccgc ttccgtcatg gcaatatttg
    10261 ctgcgaatga cccgtgcatg cctcccattc caagaaagag tgggtgactc gttgcaatcg
    10321 ttccttgtcc caaaagactg gttaccactg gaatttgata gcgttctgca aattcattta
    10381 gttccgtagc agcctcagca taactaattc caccaccagc taacaagact ggctttttag
    10441 ccttggacaa ttgcttcaag attttcttga tttgcatatc attcggctca agagtcggct
    10501 gataacttgg taaattcact tctggtgaat aaatgaagtc tgtttctaaa gcagatatgt
    10561 cttttggtag gtcaattaca actggccctg gacggcctgt agttgcgata tggacagctt
    10621 ccgtaatgat acgcggaata tcagctgtct cacgaacttg gtaattgtac ttagtgattg
    10681 gcatggtaat tcctacgatg tctgcctcct gaaaggcatc cttcccaatc cctgctcgcg
    10741 ccacctgacc tgtaaagacc aaaaggggaa cgctatcgct catagcatcc gcaatccctg
    10801 taatggcatt tgttgctcct ggtccactag tgacgacggc aacacccaac tttccagttg
    10861 atttggcata gccttcagct tcatgcaaac aaccttgctc atggcgccct agaatgtggc
    10921 gaatgccttt aaaattatat atcgcatcat aaaaaggcaa aaccgcacca ccaggataac
    10981 caaagatggt atcaactcct aaatcacgaa gtgtttccaa aactaggtcc gaccccgtct
    11041 taggagattc taaactgatt ttctccatta ttcccctttc tttcctctta aaaataactt
    11101 gttactatca taccattttt tcaaaatttt tcaagataaa agaagaaatt ttctgaattt
    11161 tctattttaa cgtttattta tgaatgttat ttttgttttt attaaaaaga taccgatttc
    11221 atttactaaa aagaaaaaag aactgatttc tcagtccttc attaatctta ttccacacta
    11281 aataggtatg ggtaaacagg ttgttgacct tggtgaatct cgacttcaac gtcttcgaat
    11341 tcttctacga tttcttgagc gatttcattg gcaagttctt cgcttccgtc ttcaccgaca
    11401 tagaaggtta cgatttcact gtcttcatcc aacatatgtt tcaaggtttc agtcaatgtt
    11461 tggtgcatat cagggtttga cacaagaatt tttccatcca ccatacctaa attatcgttt
    11521 tcatggattt ctaagccatc gatcgttgta tcacgcacgg ctgttgtgac gcttccgcta
    11581 acgacatcgc taagagcagc tgtcatacgc tcttggtttt cttcaatgga cttgcttgga
    11641 tcaaaggcaa gaagacttgt cataccttga ggaagagtgc gagcctctac cactaccgct
    11701 ggttgctcca aaacttctgc cgcagattga gctgccatga agatgttctt gttgtttggc
    11761 aagaagatga tgttacgggc attaacctgt tcaacagcct tgataaagtc ttctgttgaa
    11821 gggttcatgg tttgaccgcc ttcgataaca taatccacgc cttgagaaca gaagatatct
    11881 gctagacctt taccagccac cacagcaatc aaagcatact ctttttcttc agccgacttg
    11941 ataacttgag tagcttcttt ctcaacctgt gcttcgtgtt ggttacgcat attgtcaact
    12001 tttaccttga ccaagctacc atatttgaga ccttcttgca taacaagtcc tggatcttct
    12061 gtatgaacat ggactttgac aatttcatca tcgttaacaa cgaggagaga atctccaagc
    12121 tcatccaagt agttacggaa ttcatcgtag tcaaaatctt tagcataggt tggaccttgc
    12181 ttaagagcta ccatgatttc agtacagtaa ccaaacgtga tgtcctcagt cgctacgtga
    12241 ccagctacag acttatgatg ctctacattg atcatctcac tcatgttggc aggagtcgct
    12301 acaaagtcct cagatgcaat atattcgcca gtaagggctg aaaggaaacc ttcgtagatg
    12361 aagaccaatc cttgaccacc tgagtccaca acgccaactt ctttcaatac tggaagcatg
    12421 tctggtgttt tagctagagc tgttttagca ccttccaagg ctgcgcgcat gacttcaaca
    12481 gtgtcatctg tttgctcagc ttttttctta gcaccgatag cagctccacg agaaactgtc
    12541 aaaatcgttc cttcaacagg tttcatcact gccttatagg caacttccac acctgattgg
    12601 aaggccagag ccaagtcttg acctgttaac tcgtctttat ccttgatagc ttgggaaaat
    12661 ccacggaaaa gctgagacgt aatcactcct gagttcccac gcgcacccat caaaagccct
    12721 ttggcaagaa tgctcgctac ttctccaact gtagaagctg gcttgtctgc aacttcttta
    12781 gcaccatttt caatggtcat tcccatattt gtcccagtat ctccatctgg aactggaaag
    12841 acgtttaatg aattgacata ttcagcttgc ttattcaagc gagttgatgc agcctgcacc
    12901 atttcttgaa ataagctagt agtaattttt gacacggtta ttctcctaca actttgatat
    12961 tttgaatgta gacatttaca gtctgagcag taattccaag ctggttttcc aagctaaagg
    13021 caacacgctc ttgaatgttt tttgacactt cactaatctt tgttccgtag cttaacacgg
    13081 tatatacatc aactgcaata ctgccatctt cggctgcctt tacgacgaca cctttagaat
    13141 aattttcctt acctagcagg gcttggaaat tatctttgag ggcattttta ctagccatac
    13201 cgaccacacc aaaaatctca gttgctgctc cacctacgac ggttgcaatc acttcatctg
    13261 ttagttcgat ttgaccatct tttgtattaa tttttacagt catccttttt acctcaacta
    13321 gttgatactc tattttatca tatttcagcc caagtgtaaa agcagaatga tgtatcagcg
    13381 gatatttact ctatttttca aacgatgtta tacccacaat aaaagaaaaa agaccctaag
    13441 gtctcctttg cttttattat taaacgcgtt caactttacc tgatttcaaa gcacgagctg
    13501 aagcccaaac ttttttaggt ttaccatcga taagaacagt aactttttga aggtttggtt
    13561 ttacggcacg ttttgtttgg ttcatcgcgt gtgaacggtt gtttcctgat acagtcttac
    13621 gacctgtaaa gtaacatact ttagccattg tgttttcctc ctattagatc taatatagcg
    13681 gatgtgctag caccacatac cgtactatgt tatcacactt tcttgttttt tgcaagggaa
    13741 ttggaagatt ttttatttgt gtcttaaatc aggtcttgcg tgacattttc tgctctccac
    13801 atgccattgt tgattaacag aacaccagaa ttaaaattat gtgtataaaa atcatctcta
    13861 actgcagcta agggtatagc cgtcaagtcc aaatcccaca gctcatctat cgattttctt
    13921 acaacaatat ctgaatccaa atacagtaca cgagactcgc ttacatactt tggaataaaa
    13981 tacctaaaaa aagccgcata tgaaagtccc tcaaagggga gacgataacc tttcagaata
    14041 ctactgtcaa tctgaacatt cacaatctca ctattcaaag tctctagtct tttttccatc
    14101 aattggaacc attctcgcgg aaggtcatca ttaaaaacat aaaacttaag attataatga
    14161 tgaacacaaa gagattttat tgttgtttca actttatcca tataagcatt atctgcacct
    14221 aagacaatcg cttttttctc ttctttcact ctttatctta tttcttttta ttcccatcat
    14281 attattccca tcatatgttt ctacgtaacc attattttcg cctattcgtt cgtaaaacca
    14341 taccagtgga gattttagat gaagtcccat tacggtttac aatttttaca ttacgacacg
    14401 gagttttaca aatcgatttc atttgccaaa cgtagttagt gaggcagtta gctagttcgc
    14461 caaatagcga ctagcgtcca acaatttgga actttagctc caattgttgg tactgagtca
    14521 catcttctcc tctaactcta cgtctggata cttgtccgca aaccagcgga gggcaaagtc
    14581 attttcaaag agaaagactg gttggtcaaa acggtctttg gctaagatat tgcgacttga
    14641 cgacatccgt tcatccaagt cctcaggctt gatccaacga acggtctttt tacccattgg
    14701 gctcatgacc acttccgcat tgtattcgcc ttccatacgg tgtttaaaga cttcaaactg
    14761 gagttgacca acagctccca gcatgtactc acctgtttgg taattcttat aaagctgaac
    14821 ggctccttct tgcaccaatt gctcaatccc cttgtggaag gatttttgct tcataacatt
    14881 cttggcagaa actttcatga aaatttcagg agtaaaggtt ggcagtggtt caaattcaaa
    14941 cttgtttttt ccaaccgtca aggtatcccc aacctgataa gtaccggtat cgtaaacccc
    15001 gataatatca cctgccacgg cattgatcac attctcacga ctttccgcca taaactgggt
    15061 aacattagat agtttagccc ccttaccagt acgagggaga ttgacactca tgccgcgctc
    15121 aaattcgcct gatacgatac ggacaaaggc aatacggtca cggtgacgag gatccatgtt
    15181 ggcttggatt ttaaagacaa agcctgagaa atccttgtca taaggatcca caatttcacc
    15241 gtctgttttc ttgtgaccat gtggttctgg agcaaacttg aggaagattt caaggaaggt
    15301 ctgcacacca aagtttgtca gggctgaacc gaaaaagaca ggcgtcaatt ctccagccag
    15361 aatagcttcc tctgaaaact cattcccagc ttcatttaaa agctcaatgt catccttgac
    15421 ttgctcgtag aaaggattgc taccaaagag tttgtccccg tcttctagac tggcaaaacg
    15481 ctcatcccct ttgtaaagct ctaaacgttg gttatagagg tcatacaagc cctcaaaggc
    15541 tttccccatc ccgataggcc agttcatagg gtagctagca atgcccaaga tttcttccaa
    15601 ttcttgcaag agatccaaag gctcacgacc gtcacggtcc agcttgttca taaaggtaaa
    15661 aactggaatg ccacgatgtt tcacaacctc aaacaatttc ttggtttgag cctcgatccc
    15721 cttggcagag tccacgacca tgaccgcagc atccaccgcc atcaaggtac gataggtatc
    15781 ttctgagaag tcctcgtgcc ctggcgtgtc taagatattc acgcgcttgc cgtcgtagtc
    15841 aaattgcata acagatgaag taacagaaat cccacgttgc ttctcgatat ccatccagtc
    15901 agatttagca aaagtccctg ttttcttccc ttttaccgta ccagcctcac gaatctcacc
    15961 cccaaagtag agtaactgct cagtgatggt tgttttcccc gcgtccgggt gggagataat
    16021 ggcaaaggta cgacgtttct taatttcttc ttgaatattc ataagttctc tttctttgat
    16081 tctctatttt tcttgtttca atagctgaga atgattttta cattggattt taccattcct
    16141 ttcaatactc cattatatcg gattttagca tttttttcaa tttctatctc ttttcacttc
    16201 cccctccctt atttatagga aaatatggta aaatagaaca gactaaaaat catcatttca
    16261 cgaaaggatg caagatgaaa attacgcaag aagaggtaac acacgttgcc aatctttcaa
    16321 aattaagatt ctctgaagaa gaaactgctg cctttgcgac caccttgtct aagattgttg
    16381 acatggttga attgctgggc gaagttgaca caactggtgt cgcacctact acgactatgg
    16441 ctgaccgcaa gactgtactc cgccctgatg tggccgaaga aggaacagac cgtgatcgct
    16501 tgtttaaaaa cgtacctgaa caagacaact actatatcaa ggtgccagct atcctagacg
    16561 atggaggaga tgcctgatga cttttaacaa taaaactatt gaagagttgc acaatctcct
    16621 tgtctctaag gaaatttctg caacagaatt gacccaagca acacttgaaa atatcaagtc
    16681 tcgtgaggaa gccctcaatt catttgtcac catcgctgag gagcaagctc ttgttcaagc
    16741 taaagccatt gatgaagctg gaattgatgc tgacaatgtc ctttcaggaa ttccacttgc
    16801 tgttaaggat aacatctcta cagacggtat tctcacaact gctgcctcaa aaatgctcta
    16861 caactatgag ccaatctttg atgcgacagc tgttgccaat gcaaaaacca agggcatgat
    16921 tgtcgttgga aagaccaaca tggacgaatt tgctatgggt ggttcaggtg aaacttcaca
    16981 ctacggagca actaaaaacg cttggaacca cagcaaggtt cctggtgggt catcaagtgg
    17041 ttctgccgca gctgtagcct caggacaagt tcgcttgtca cttggttctg atactggtgg
    17101 ttccatccgc caacctgctg ccttcaacgg aatcgttggt ctcaaaccaa cctacggaac
    17161 agtttcacgt ttcggtctca ttgcctttgg tagctcatta gaccagattg gaccttttgc
    17221 tcctactgtt aaggaaaatg ccctcttgct caacgctatt gccagcgaag atgctaaaga
    17281 ctctacttct gctcctgtcc gcatcgccga ctttacttca aaaatcggcc aagacatcaa
    17341 gggtatgaaa atcgctttgc ctaaggaata cctaggcgaa ggaattgatc cagaggttaa
    17401 ggaaacaatc ttaaacgcgg ccaaacactt tgaaaaattg ggtgctatcg tcgaagaagt
    17461 cagccttcct cactctaaat acggtgttgc cgtttattac atcatcgctt catcagaagc
    17521 ttcatcaaac ttgcaacgct tcgacggtat ccgttacggc tatcgcgcag aagatgcaac
    17581 caaccttgat gaaatctatg taaacagccg aagccaaggt tttggtgaag aggtaaaacg
    17641 tcgtatcatg ctgggtactt tcagtctttc atcaggttac tatgatgcct actacaaaaa
    17701 ggctggtcaa gtccgtaccc tcatcattca agatttcgaa aaagtcttcg cggattacga
    17761 tttgattttg ggtccaactg ctccaagtgt tgcctatgac ttggattctc tcaaccatga
    17821 cccagttgcc atgtacttag ccgacctatt gaccatacct gtaaacttgg caggactgcc
    17881 tggaatttcg attcctgctg gattctctca aggtctacct gtcggactcc aattgattgg
    17941 tcccaagtac tctgaggaaa ccatttacca agctgctgct gcttttgaag caacaacaga
    18001 ctaccacaaa caacaacccg tgatttttgg aggtgacaac taatgaactt tgaaacagtc
    18061 atcggacttg aagtccacgt agagctcaac accaattcaa aaatcttctc acctacttct
    18121 gcccactttg gaaatgacca aaatgccaac actaacgtga ttgactggtc tttcccagga
    18181 gttctaccag ttctcaataa aggggttgtt gatgccggta tcaaggctgc tcttgccctc
    18241 aacatggaca tccacaaaaa gatgcacttt gaccgcaaga actacttcta tcctgataac
    18301 cccaaagcct accaaatttc tcagtttgat gaaccaatcg gatataatgg ctggattgaa
    18361 gtcaaactag aagacggtac gaccaagaaa atcggtatcg aacgtgccca cctagaggaa
    18421 gacgctggta aaaacaccca tggtacagat ggctactctt atgttgacct caaccgccaa
    18481 ggggttccct tgattgagat tgtatctgag gcagatatgc gttctcctga agaagcctat
    18541 gcttatctga cagccctcaa ggaagttatc cagtacgctg gcatttctga cgttaagatg
    18601 gaggaaggtt cgatgcgtgt ggatgccaac atctcccttc gtccttatgg tcaagagaaa
    18661 ttcggtacca agactgaatt gaagaacctc aactccttct caaacgttcg taaaggtctt
    18721 gaatacgaag tccaacgcca ggctgaaatt cttcgctcag gtggtcaaat ccgccaagaa
    18781 acacgccgtt acgatgaagc gaataaagca accatcctca tgcgtgtcaa ggaaggggct
    18841 gctgactacc gctacttccc agaaccagac ctacccctct ttgaaatttc tgacgagtgg
    18901 attgaggaaa tgcggactga gttgccagag tttccaaaag aacgtcgtgc gcgttatgta
    18961 tctgaccttg gtttatcaga ctacgatgct agtcagttga ctgctaataa agtcacttct
    19021 gacttctttg aaaaagctgt tgccctaggt ggtgatgcca aacaagtctc taactggctc
    19081 caaggggaag tcgctcagtt cttgaatgct gaaggtaaaa cactggaaca aatcgaattg
    19141 acaccagaaa acttggttga aatgattgcc atcatcgaag acggtactat ttcatctaag
    19201 attgccaaga aagtctttgt ccatctagct aaaaatggcg gtggcgcgcg tgaatacgtg
    19261 gaaaaagcag gtatggttca aatttcagat ccagctatct tgatcccaat catccaccaa
    19321 gtctttgccg ataacgaagc tgctgttgcc gacttcaagt caggcaaacg taacgccgac
    19381 aaagccttta caggattcct tatgaaggca accaaaggcc aagccaaccc acaagttgcc
    19441 cttaaactac ttgcacagga attggcgaag ttgaaagaaa actagacaga acaaaaccag
    19501 ccctaaggtt ggttttttct tctctaccaa ctcccaataa ctattttggc tttatttcca
    19561 gagtatttta tggtaaaatg aagagtaata atatttatta aagaggtaaa aacatgattg
    19621 aagcaagtaa attaaaagct ggtatgacct ttgaaacagc tgacggcaaa ttgattcgcg
    19681 ttttggaagc tagtcaccac aaaccaggta aaggaaacac gatcatgcgt atgaaattgc
    19741 gtgatgtccg tactggttct acatttgaca caagctaccg tccagaggaa aaatttgaac
    19801 aagctattat cgagactgtc ccagctcaat acttgtacaa aatggatgac acagcatact
    19861 tcatgaatac agaaacttat gaccaatacg aaatccctgt agtcaatgtt gaaaacgaat
    19921 tgctttacat ccttgaaaac tctgatgtga aaatccaatt ctacggaact gaagtgatcg
    19981 gtgtcaccgt tcctactact gttgagttga cagttgctga aactcaacca tctatcaaag
    20041 gtgctactgt tacaggttct ggtaaaccag caacgatgga aactggactt gtcgtaaacg
    20101 ttccagactt catcgaagca ggacaaaaac tcgttatcaa cactgcagaa ggaacttacg
    20161 tttctcgtgc ctaatctcta gaaagaggtc attctatggg aattgaagaa caacttggcg
    20221 aaatcgttat cgccccacgt gtacttgaaa aaatcattgc tatcgctact gcaaaggtag
    20281 agggtgttca ctctttttca aacagatcag tgtctgatac cctttcaaaa ctttcactcg
    20341 gccgtggcat ttatcttaaa aacgtggacg aagaactcac agcagatatc tatctctacc
    20401 ttgagtacgg agtaaaagtt cctaaggtag cggttgctat ccagaaagct gtcaaagatg
    20461 ccgtccgtaa tatggctgat gtagaactcg ctgctatcaa tattcacgtt gcaggtatcg
    20521 tcccagataa aacaccaaaa ccagaattga aagatctatt tgacgaggac ttcctcaatg
    20581 actagtccac tattagaatc tagacgccaa ctccgtaaat gcgcttttca agctctcatg
    20641 agccttgagt tcggtacgga tgtcgaaact gcttgtcgtt tcgcctatac tcatgatcgt
    20701 gaagatacgg ctgtacaact tccagccttt ttgatagacc tcgtttctgg tgttcaagct
    20761 aaaaaggaag aactagataa gcaaatcact cagcatttaa aagcaggttg gaccattgaa
    20821 cgcttaacgc tcgtggagag aaacctcctt cgcttgggag tctttgaaat cacttcattt
    20881 gacactcctc agctggttgc tgttaatgaa gctatcgagc ttgcaaagga cttctccgat
    20941 caaaaatctg cccgttttat caatggactg ctcagccagt ttgtaacaga agaacaataa
    21001 aaagaaaaag tgtttgatat ctatcaaaca ctttttcttt gcctcccact atctaaaaaa
    21061 atgataatag atataattgt aaacaaaaat ccagataggt tttgcatgat tgagaaagtt
    21121 aaaaaaacta tggcagagaa tcgttaatct cagattgtcg gtagaacgat aaacaagggc
    21181 aaaaaagaaa ccaatcagac tataatataa taaactaatt ggatctctgt gagatagtat
    21241 caaatgacta agcccaaaga taaggcctga taagatgaca tctaaataat acttggacta
    21301 gggaaagaag gtattcataa aataccctct atcaagagtc tcctcaaaaa caggaccgat
    21361 gattacaggc aggacaaaag ataagatagt cgataaaaag gttggttgtc catttgaaag
    21421 aaccacggta aaatactcat catgaatatt cctatgatta atcaaatgag catagcgtgc
    21481 ccaaaaatta ccgagaatct gataaaccac ataagttgca aataaggaga agacaaatga
    21541 ccagttccag ctctttttct tttttaacct ccaaattaat agaaaaggaa acttcccact
    21601 aatcccactg ttaaaataag agaatagaca tcagctccta accctaaaat gatcgtcaca
    21661 tacaatccaa ttgtttgtgg taaataggta gatagtaaaa taataagcaa aaatattcca
    21721 aattgtctta gtttctttgt gtttctcatc gtactttttt gaaagattac cctgttcgga
    21781 agccgtactt ccaagcatct atataagaat taagtgcccc ttgcctcata tagggagcaa
    21841 attctctata atataaccat ctactatatc catcttccca aacagcaaga ccacctgaag
    21901 tttgctccaa gtcctcagtt gaaataactg taaatgtttc tgtacctgtc attgcaagta
    21961 ccttcttaaa atagattgtt gtaggctcac atttatagta tatttctttt tttgtctatt
    22021 ttatagtcta tctcctcaac tggcaatttt tcgacctgaa ttgcattttt ccataaaaaa
    22081 tgagaccttt ctagtctcat ttagtcattc ttagtatttt ctaaatcgtt gatagcgttc
    22141 ttccagcaac tcttctagcg gtttttgtga aagtctagcc agctccgttt ggagttcttt
    22201 tttgacactc ttaattagtt ctttactaga aagtcctact tcagaaatca ccttatccac
    22261 cacgtccatt tctaacagtt catgcgaagt gattttcatc agttctgctg cttccatggc
    22321 gcgagtaccg tccttccata aaatggaagc aaagccttct ggactgagaa tggcatagat
    22381 agaattttcc agcatccaga cacggtccgc gacagctaga gccagagccc cgcctgaacc
    22441 accttcaccg ataataatgg cgataatagg aactttcagg tcactcattt ccatgagatt
    22501 gcgagcgata gcttcccctt gaccacgttc ttccgctccg acaccaggat aagcacctgc
    22561 tgtattgata aaggtcacaa ctggacggcc aaatttctca gcctgtttca tcaaccgcag
    22621 tgcctttcgg tagccttctg gatgtggttg gccaaaattc cgtttgaggt tgtcttgcaa
    22681 actcttgcct ttttggatac caaccactgt tacagcttgg tctccaagcc aaccaatacc
    22741 accaacaact gcaccatcat cacgaaaaga acggtcacca tgtaattgga taaattcatc
    22801 aaaaatgcct gtcgcaaagt ccaaggttgt caagcgactc tgctcacgcg cttctctgac
    22861 tatttttgca atattcatct aggactccct ccatgcaatc tgactaggct agcaatcgta
    22921 tctggtaagt ctcttctttt gacaatagca tccacaaagc catgttctaa taggaattct
    22981 gccttttgga aatcctcagg caagctttca cgaaccgtat tttcaatcac acgacgccca
    23041 gcaaaaccaa ccaagctctg tggttcagcc agaatgatat cgccttccat agcgaaagaa
    23101 gctgtcacac caccagtcgt tggatctgtc aaaatggtca ggtaaaagag accagcattt
    23161 gaatggcgtt taaccgctgc agagatctta gccatctgca tgagactcat gattccttcc
    23221 tgcatacggg ctccaccaga ggctgtgaat aggacaactg gcaatttttc gacagtcgca
    23281 tactcaaaca aacgagtgat tttttcacct acaaccgtac ccatagaagc catgataaaa
    23341 ttagaatcca taatcccaag agccacagtc tgacctttaa taagagcagt tcctgtcaca
    23401 acggcttcat gcagacctgt tttttcacgc atagatgcca gtttcttttg gtaaccaggg
    23461 aaatgcaagg gatccttgct ttcaatccct gtaaacaatt ctttgaaggt tcccatatca
    23521 atcgtcaaag ccaagcgttc ttgggcagaa atacgaaagg tatagctaca gtgcggacag
    23581 atacgttcac ttcccagatc cttctgatag atggtatgct tacagcctgg acactgggaa
    23641 aataattcat ctggaacctc tggcttagct tgaggttttt ccctaaccga acgattggga
    23701 ttgattcgaa tatacttatc ttttttacta aatagagcca ttgattcccc ttttcggttt
    23761 aaactcttaa agtcatttta ttctttttct tgatatttag gtaagaaggt ttccatcaag
    23821 aaggaagtat cataatcccc agcaatgaca ttgcgatctg aaatgagatc aagctggaaa
    23881 tctgcattgg tctgcactcc ttcaatctct aattcataga gggcacgttg cattttcatc
    23941 aaggcgtcaa aacgattttc gccgtgtact atgattttgg caatcatact atcataataa
    24001 ggcggaatgg tataacctgg ataaactgct gaatccacgc gcaagccaac tccaccactt
    24061 ggcagataga gattagtaat cttacctgga cttggagcaa agttaaaggc tgggttttct
    24121 gcattgatac gacactcgat ggcatgaccg cgtaggacaa tatcttcttg cttaacagac
    24181 aaaggctgac ctgccgcaat gcaaatctgt tccttaacga tatcaacacc tgaaacaaac
    24241 tctgttactg gatgttctac ctgaacacga gtattcatct ccatgaaata gaaatttcta
    24301 cttgcttcat caagaagaaa ttcaatggtt cctgcattct catagccaac aaactctgcc
    24361 gctcgaacag cagcagcacc tatttcatga cgcagcgttt ttccgattgc aatcgaggga
    24421 ctttcttcca aaaccttttg gttattcctt tgaagagaac aatcccgttc acccaagtga
    24481 atcacatgtc catgctcatc acctaggatt tgaacctcaa tgtgccgagc tggatagata
    24541 acccgttcta tgtacatggc accattgcca taattggcct tggcctcact agaggcagtt
    24601 tcaaaggcag aaacgaggtc atctggtttt tcaaccttac gaatcccttt accacctcca
    24661 cctgctgaag ccttgagcat aacaggatag ccaatttttt cagcaacaat caaagcttct
    24721 tcagagttat gcacttctcc atctgaacct ggtataacag gcacacctgc tttaatcatc
    24781 tgagcacgcg cattgatctt atcccccatc atatccataa catgaccaga tggaccgata
    24841 aacttgatac ctacttcttc acacatggtc gcaaatttgg aattttcact gagaaatcca
    24901 aaaccagggt gaatagcttc tgcctcagtc aagactgcag ctgatagaac tgcattaata
    24961 ttgagataag actctgttgc cttgccagga ccaatacaaa ctgcttcatc tgccaaaagc
    25021 gtatgaagag cttccttatc agcagttgaa taaaccgcta ccgtcgcaat ccccaattca
    25081 cgtgccgcac ggataatacg aaccgcaatt tcaccacgat tggcaattaa aatttttcga
    25141 aacatggaga acctccttag ttcccaattg caaaagtaag gataccactg gctgcaagct
    25201 tgccatccac ttcagccttt gcttcaacca cagctatggt gccacgacgt tttacaaaag
    25261 tcgctgtcat aaccaattgg tcgcctggta caacttgctt cttgaactta accttgtcca
    25321 taccagcgta aaagaccagt tttcctttat tttcaggttt tgataactcc aacacaccag
    25381 cagtttgcgc caaggcttcc ataatcagaa cacctggcat aactgggtat tgaggaaagt
    25441 ggccgttaaa gaaaggttcg ttaatggtca catttttgat agcaacaatg gtatcctcgc
    25501 tcacttccaa gacacggtcc actagaagca taggataacg gtggggaaga gcttctttga
    25561 ttccttgaat atcgatcatt tgatacgtac caatccttta ccaaactcaa ccatttcttc
    25621 gttagagacg agaatttccg ttaccacacc atccttagga gctgggattt cattcatgac
    25681 tttcatggct tcgataatta ccaatgtttg accttttttg acactatcac caactgtaac
    25741 gaaggcaggt ttatctggtc cagcagccaa gtaaaccact ccaacaagtg gactctctac
    25801 aagatttccc tcagtagcca cacttgcttc agctggagct ggaacttctt ctgctacagt
    25861 ctctgctgga gcagatgtag gagctactgg actcggtgtt gctagaacgg gtgctggagc
    25921 gacttgagtt gcaacttcag gcacaggtct tgcttcattc ttgctaaact gcaactcatc
    25981 cgtcccattt ttataagaaa attctctcaa acttgactgg tcaaattgag tcatcaagtc
    26041 tttaatatcg tttaaattca tacttatcta ttctcccaac gtttgaaagc aagaactgca
    26101 ttgtggcctc caaaaccaaa agtatttgaa atagcgtatg gaatttcttt ctccaaacct
    26161 tgtccataaa cgacattagc ttcgatataa tctgatactt cacttgtccc agctgtcatt
    26221 ggtacaaagt tatgacgcat agcttcgatg gtgacgatag cttctactgc acccgcagcc
    26281 cccagcaaat gtcctgtaaa agacttggtt gatgatacag gtacttcctt accaagaaca
    26341 gctacgatag caccactttc tcctttttca ttggcaggag ttgacgttcc gtgagcatta
    26401 acataggcta cttgctctgg agaaatctca gcttcttcca aggctagttt gatggccttg
    26461 atagctccct gaccttctgg atgtggagaa gtcatgtggt aggcatcaca agtatttccg
    26521 taaccaacca cttcagccag gatagtagct ccacgttttt cagcgtgttc aagactttct
    26581 agaaccaaca tccctgaacc ttcacccata acaaacccat tgcgatcctt atcaaatggg
    26641 atcgaagcac gagttggatc ctctgtagta gagagagctg ttaaggcttg gaaaccagcg
    26701 atggcaaaag gtgtgataga agcttctgtt cctcccacca acatcacatc ttggaaacca
    26761 aacttaatgg agcggaaggc atccccaatc gcatcatttg atgaagagca ggcagtattg
    26821 atagatttac aaacaccgtt tgcaccaaaa cgcatggcta cattcccaga agccatattt
    26881 ggtaaagctt ttggaagagt cattggtttg acacgtttgg gtcctttttc atgaaggcga
    26941 agtacctgat cttcaatttc cttgattcca ccaataccag atgcaacgat aacaccaaaa
    27001 cgatccctat taagagcctc tacatcaaga ttggcatggt ttacagcctc ttgggctgca
    27061 tacaaggcat ataaagaata gttatcaaaa cggttggtat ctttttttac aaagtattta
    27121 tcgaacggaa aatcttggat ttctgccgca ttatgcacat caaagtcact atgatcaaat
    27181 tttgtaatgc caccaatgcc gattttccca gttgctaaac tattccaaaa ttcttctggt
    27241 gtatttccga ttggagatgt tactccataa cctgttacca ctactcgatt tagtttcatt
    27301 cttttcacct ctagctttcg ctacatactt aagccaccat caatggcaat cacttgacca
    27361 gttagataat cttggcctgc taaaaatact gtcaaatctg caacctgctc tgcctgccca
    27421 aattctttca tcggaatctg agctagtgta gcttccttaa tcttatctga taagatagct
    27481 gtcatatcag actcaatcat tcctggagca atcacattga ctcgtatatt ccgactagcg
    27541 acctcgcgtg ccacagactt ggtaaagcca atcaagccag ccttagaagc agcatagtta
    27601 gcttgaccaa tattccccat caaaccaaca acactagaca tattaatgat agcaccttct
    27661 ctggctttca tcatcggttt caagactgat tgtgtcatat taaaggcacc agtcagattg
    27721 accttgagca ctttttcaaa atctgcttct gtcatcttga gcataagagt atcttgggta
    27781 atccctgcat tgttgaccaa aacatctact gaacccagtt ctgcaatagc ttgatcaatc
    27841 atacgcttag cgtctgcaaa atctgataca tctcctgaaa tgggaaccac cttgatacca
    27901 tagtttgaaa actcagcgag caattcttct gagattgccc cacgactgtt taagacaatg
    27961 ttggctcctg cttgagcaaa cttgtgggcg atggcaagac caattccacg actcgaacct
    28021 gtaataaaga tatttttatg ttctagtttc atttttttcc tttcaaaact tctacttatt
    28081 ttagtctatt tttttaaaag tgctactaaa ctcgcttgat cttccacatg agctaagtga
    28141 gtagtttgat caattttttt aacaaaacct gacaagactt tccccggtcc aatctcgata
    28201 aagttgctta tgcctgcttc ttgcatgacc ccaatacttt catagaaacg aacgggttcc
    28261 ttgacctgac gcgtcaagag ctgagcaatg tcctcttttt gcatcacagc agcttctgta
    28321 ttgccgacta ggggacaagt aaaatctgaa aaacttacct gagccagagt ttcagctagt
    28381 ttctggctag caggttcaag gagagaggtg tgaaagggac ctgacacctt aagaggaatc
    28441 aagcgtttgg cacctgcttc ttgcaaaagt tcaaccgctc gatcaactgc aaccacttct
    28501 ccagcaatga cgatttgtgc aggtgtgtta tagttggctg gagtaaccac tccaagttca
    28561 gaagcttttt gacaggcttc ttcaatgacc tctactggcg tattgagaac tgctaccatc
    28621 ttgccagagt cagcaggagc cgcttcttcc atataggctc cacgcttagc taccaaggca
    28681 accgcatctt caaaatccaa ggcgccgctt gccaccaagg cagagtattc tccaagagac
    28741 aaaccagcaa ccatatcagg ctgatagccc ttttcttgca ataaacggta gatagcaacc
    28801 gaagtcgcta gaatggctgg ttgcgtatag cgggtctgat tgagtttgtc ttcttccgta
    28861 tcgatgagat aacgcaaatc ataacctagc acctgactcg ctcgatcaat cgtttcttta
    28921 acaatcggat actgatcata gaaatcccgt cccatcccta gatactgggc accttgacca
    28981 gcaaataaaa aggctgtttt agtcatttct tacaactcct gtccagcgag aggcttcttc
    29041 ttgaattttc ttagcggctc cgtaatacaa atcttttagg atttcttcag ctgtttcttc
    29101 tttagaaaca agccctgcga tttgacctgc catgacagag ccaccctcca catcaccgtg
    29161 aacaactgct ttggctaggg cacctgctcc catttgttca aagatttcta aatcaggatc
    29221 ttcctgctta aaggcatctt tttcagccag ttcaaaatct ctagtcaact gatttttaat
    29281 agcacgaaca gcatgaccaa agtgctgagc tgaaatcgta gtgtcaatat cccttgcttt
    29341 taaaattttc tccttgtagt ttggatgggc attcgactct tttgcaacta caaaccgtgt
    29401 ccccacctgt acagcctctg cacctagcat aaagccagcc gcagcacctt caccatccgc
    29461 aattcctcct gcagcaataa caggaataga tacagctgtg gctacctgtc gcaccaaggt
    29521 catggttgtt aatttaccga tatgcccccc agcttccatt ccttctgcaa taacagcgtc
    29581 tgcaccgatt ttttccatgc gtttagctaa agcgacacta ggaacgacag gaataacgat
    29641 tatcccagct tcatggaaac gttccatata cttgcttgga tttcctgctc ctgttgtgac
    29701 aactttaaca ccttcttcaa taacgagatc cacgatatct tccacaaagg gagataagag
    29761 catgatgttg accccaaagg gtttatcagt caatgatttg attttatcaa tattggcctt
    29821 gacaacttct ttcggggcat ttcccccacc gataattcct aatcctccag ccttggaaac
    29881 agcccctgcc aaatcaccat cagcaaccca ggccatccct ccttggaaaa taggatagtc
    29941 aatcttcaat aattctgtaa tacgcgtttt catagtgcct ccaaccttcc ttgcttacgt
    30001 aatagttcga tttcaccata atttgacagt caaactatta cctaaacaag agggagtggg
    30061 tttctcccta ctccttctac taatattctg cttattttgc ttgctcttca acgtaagcaa
    30121 ccaagtcacc aactgttttc aagtcatttt ctgcttcgat ttggatatca aaagcatctt
    30181 cgatttctga gattacttgg aacaagtcca atgaatctgc gtccaaatca tcaaaagttg
    30241 attcaagtgt tacttctgat gcgtcttttc caagttcttc aacgataatt tcttgtactt
    30301 tttcaaatac tgccatgata ggactccttt aaaataaata gtttttttat aacaatgtgt
    30361 tcaccacatg attacctaaa ttgtaagaat gagcgtgccc caggtcaaac ctccaccgaa
    30421 gcctgataga agaacagtct ggctaccatc taaagggatg agaccttgtt ctacacactc
    30481 tgaaagtaaa atcgggatac tggctgcact ggtattgcca tattccatca tattggctgg
    30541 aagtttggct cggtcaacac caatttttct agccatctta tccaaaatac ggtcattggc
    30601 ttgatgaagt agcagataat ccaagtctgt cacctctata ggagattcat caatagtctg
    30661 cttgatagac ttggctacat ctcgaatggc aaaatcaaag actgtgcgtc catccatctt
    30721 caaaaacgaa tctgcacttt cttgatctga aaatggagaa tgcaaacctg aatgcccata
    30781 agttaaacac tcgctgcgac ttccatcgct attgagactc tcagctaaga aatgctcttg
    30841 ctcgctagct tctaacaaga caccaccagc accatctcca aacaacacag ctgttgatcg
    30901 atccgaccaa tcgactgcct tagagagggt ttcactacca atcaccaagc ctttttgaaa
    30961 gcgaccagaa gcgataaact tttcagcagt tgaaagagca aatacaaatc cactgcaagc
    31021 cgcggttaag tcaaaagcaa aggctttatt agcaccaata ttagcttgaa cacgagcagc
    31081 tgtagagggc atcatcgaat ctggagtaat ggtagctagg atgataaaat ccagttcttc
    31141 tcctgttatt ccagcttttg ccatcagttt cttagcaacc tctgtagcca aatcactggt
    31201 agattctgtt cttgaaatat gcctttgtcg tattcccgtt cgacttgaaa tccactcatc
    31261 attggtatcc ataatctgag ccaagtcgtg atttgtaacc acttgctctg gcacataatg
    31321 agcaacctga cttatttttg caaaagccat tatttcaaat cctccaaaaa ttggtaaaga
    31381 ttagtcaagc ctttacccat gacagcaatt tcttcctcgc tcatgccatc aataattttt
    31441 tctaccatgg ccttgtggaa gcgtttatgc agtctatgaa tcaagcgacc cttctttgtc
    31501 aaatgcagat gcaccacacg acgatcctgt tctgaccgaa ctcgctcaat gtagccctta
    31561 cgctctaaat tgttcaaact tgtcgtaaca gttccaagag ttaccattaa ctcttttgac
    31621 acttgacttg gagtcacatc tggagcctta ccgatgacat caattgtatg catttctttg
    31681 atggagatat ccttaaaacg actacctctc aagttcactt cctcaattac aaggacattg
    31741 ttaaatatag atgttaaata ttcattgatt cgttggtagt ccatttttca tatccctcct
    31801 tcttcaaaaa acttttacaa tcaaaacatt tgacagaaag aattatatca aaattcaaag
    31861 aatgatgcaa gtatttttta ttttcctgta aatttaggtc ttcttctctc cgaatgagct
    31921 cgcactcctt ctttgaaatc ctctgtttga gctaaggatt tctgtaggtt cagttctaaa
    31981 gtagcgtaac cctgccagtc tttaaattgg ctctcccaaa ccaacttctt aatggcagca
    32041 taggaattac ttgaagcacg tcttaatttt ttaagaagct gttctctcgt cttttcaagt
    32101 ttttcagctt cagaaacgcg gtaaaccaac ccccattcta gggctttttc tgccgttaaa
    32161 gcctctcctg tcatagctaa ttgagcagca cgcgtcacac cgatactgcg gctcaagaga
    32221 tgaattcccc ctgcatctgg agccaaacca acaccaacaa aggcttggat aaacttagcc
    32281 ttatccgtcg ccaaacagaa atctgccgca acagccatat tcgctgcggc acctgcaaca
    32341 gcaccgtcaa cttccattaa aacaggtttg gctatttgct tgattttata agaaatcgta
    32401 ttgaccaact ctgcgatttt tgtcaatgat ggaatatcat cctcatccac tgcccgcttc
    32461 atctctacca aatctccccc aactgagaag acctttccat tggcattgat taagataaaa
    32521 tgcacagctg gattttcttc tgccaaagtc agagcttcta aaatctcctc acacatggga
    32581 atatgaaaac catttgcgac ctcaggacgg ttcaaggtaa ggattgccaa gtcctcttca
    32641 agctgataaa taatgtgttc catcagggct cctttttatt ttataagcag tttatactct
    32701 tcgaaaatct cttcaaaccg cgtcagcttc catctgcaac ctcaaaacag tattttgagc
    32761 tgacttcgtc agtcttattt acaacctcaa agcagtgctt tgagcagtct gcggctagct
    32821 tcctagtttg ctctttgatt tcattgagtg ttaaattatt tcactgtcaa agtatatctt
    32881 ttttaggagc agaaagcaag aaaaaatcga ctgaaagtgt ctcagccgat aagaaaggct
    32941 cgctttaggc tagaaagcat cttattttct tcattgaaaa aagcctttct ttctgctata
    33001 atcgtataaa atacttactt taggagttct tatgaaagtt gttaaatttg gaggtagttc
    33061 tcttgcctct gctagtcaat tagaaaaagt tttaaacatc gtcaaaagcg attcagagcg
    33121 tcgttttgta gtcgtttctg cgcctggtaa acgcaatgct gaagatacta aggttacgga
    33181 tgccctgatt aaatactacc gcgactatgt tgcgggtaac gatattagca agagccaaag
    33241 ctggattatc gaccgctatg ctgctatggt tagtgaattg ggactaaaac cagctgtgct
    33301 agaaaaaatt tctaaaagca ttcacgcctt ggccactctt cctattgaag aaaatgaatt
    33361 tctctacgat actttcctag cagccggtga aaataacaat gccaaattga ttgctgccta
    33421 ctttaaccaa aatggtatcg atgcacgcta tatgcaccct agagaagctg ggattgtggt
    33481 cacaagtgaa cctggtcacg ctcgcatcat tccatcaagt tatgacaaga ttgaagaatt
    33541 gacaaacacc aatgaagtcc ttgtcattcc tggtttcttt ggtgtcacta aggaaaatca
    33601 aatctgtact ttctcacgtg gaggttctga tattacaggt tctatcattg ctgctggtgt
    33661 caaagccgac ctctatgaaa actttacgga cgttgatggt atctttgcag cccaccctgg
    33721 tattatccac caaccacact cgattcctga gttgacctac cgtgaaatgc gcgagttggc
    33781 ctatgcaggc ttctcagtcc ttcatgacga ggctcttctt cctgcctacc gtggaaaaat
    33841 tcctctggtt atcaagaata ccaacaaccc tgaccatcca ggtactcgta tcgttctaaa
    33901 acacagtaat gatgaatttc cagttgtggg aattgctggt gactcaggct ttgtcagcat
    33961 taacatgtcg aaatacctca tgaaccgtga ggttggattt ggccgcaagg ttctgcaaat
    34021 cctggaagaa cttaacatcg gttgggaaca tatgccaaca ggtatcgacg atctttctat
    34081 cattctccgt tctcgccaac taactcccat caaggaagaa gaaatcctgc gtcagttggt
    34141 tcaaaaggct aaagtagatc atgcagaaat cgaacacgac ctttctatca ttatgattgt
    34201 tggtgaaaaa atgaagagcc acatcggagt gactgctact gcgacacgcg ctctatctga
    34261 aaacaaaatc aacatccaga tgatgtctca aggttctagt gaagtttcta ttatgtttgt
    34321 tgtcaataaa gaccaagaga aagcagcaat taaagccctc tacaatgcct tttttggaga
    34381 aagtaaggaa gactaagcat ggcccaatca cttaacaaaa cagtactcct cagtacgaca
    34441 ggcacttcct acctctctat cgctgggaaa gttgggaaat tccttgtcgg agatcaggct
    34501 ttggaatttt acccagatgt caatgtcgaa caatttatcc agattccttg gagccatatc
    34561 aatcagattg gagccaatgt cactggacgc aaaatcagcc gccacttcga agtctttaca
    34621 gacaaaggaa aattcctctt tgcttcaaaa gactcaggtg ccattctcaa aattgcacgc
    34681 gaaaaactag gcaatgacaa ggtcgtcaaa cttccgaccc tgattcagac aattagccaa
    34741 aaatttaaaa atctatttgc aaaaaagtag aaactttagt ataatcatag caacggataa
    34801 gtaggttatc ttcttctttc agaaagtctg cgggtactgc gagcagatag gggggatagg
    34861 tgaaattcta ccgttagtaa caattacata cacaatcaag tgcacacctg tgaagttgga
    34921 tggaaccgtg gccctgccac tccaacgttt tgtcaggcgt gcttttttca taaaggagtt
    34981 cttatgttag atatcaaacg tattcgtaca gattttgaag ctgtcgcaga aaaattagct
    35041 acacgtggtg tagaggctgc tgtcttgaat gaaatgaaag aaatcgatgc taaacgtcgt
    35101 aacatcttgg tcaaggttga aactctcaaa gcagaacgta acacagtttc tgctgagatt
    35161 gcccaagcta agcgcaacaa ggaaaataca gatgacaaga ttgctgccat gcaaaatcta
    35221 tctgctgagg ttaaagcctt ggatgctgaa ttggcagaaa tcgatgctaa attgacagaa
    35281 tttacaacga ctcttccaaa tatcccagct gacagcgttc ctgttgggac tgacgaagac
    35341 gacaatgtgg aagttcgccg ttggggtact ccacgcgagt ttgacttcga acctaaagct
    35401 cactgggatc tcggtgaaga ccttggtatc cttgactggg aacgcggtgg taaggtaaca
    35461 ggcgctcgct tcctcttcta taaaggcctc ggtgctcgtt tggaacgtgc tatctacaac
    35521 tttatgttgg atgaacatgg aaaagaaggc tatactgaag tcatcacacc ttacatagtc
    35581 aaccatgatt ctatgtttgg tactggtcag tatccaaaat ttaaggaaga tacttttgaa
    35641 ctcagcgata ccaactttgt cttgattcca actgctgaag ttcctctgac aaactactac
    35701 cgtgatgaaa tcttagacgg caaagatctt ccaatctact tcactgccat gagtccgtca
    35761 ttccgttctg aggctggttc tgccggtcgt gatacgcgtg gcttgatccg tttgcaccaa
    35821 ttccacaagg ttgaaatggt caaatttgcc aaaccagaag aatcttacga agaattggaa
    35881 aaaatgacag ccaacgctga aaacattctt caaaaactca accttccata ccgtgtcgtt
    35941 gctctctcta ctggagatat gggcttctca gctgcgaaga cttacgactt ggaagtgtgg
    36001 attccagcac aaaacaatta ccgtgaaatc tcaagctgtt caaacacaga agatttccaa
    36061 gcccgtcgtg cccaaatccg ttaccgtgat gaagcagatg gcaaggtgaa actccttcat
    36121 accttgaacg gttctggact tgcagttgga cgtacagtgg ctgcaattct tgaaaattac
    36181 caaaatgaag atggttctgt gaccatccca gaagcacttc gtccatacat gggtggagct
    36241 gaagtcatca aaccataaaa aataaggttt agctatttct agctagacct tttttcgtaa
    36301 ccaaatcaga taagcaccta gtacaaagaa taaaatagtt aggcatataa tggtttcagc
    36361 caataccagg taatccagaa atggaagttt caaaattccc tgagccatct tgagcgaggt
    36421 cgctgtgata atggttggga aggtgagggc tgagaaggct ggttgaaaac cttgttttaa
    36481 aatgttgggc agacgagtta aaacaaagaa aaagaaggat tgagaagcca aaatcatgac
    36541 aatcaagacc caagtcggca ggctggttcc tcctactcga actagagaag ccaagagtag
    36601 agagaaagga gcacagtaga ttccttcttg tccaagcaag gctagtggga gtggatgttt
    36661 ctttaaatcg ctataaataa ggggatagag atagaaggtc aagagaaaac caaaactcaa
    36721 ggtcgcatag gcaatttcga taatacctac cagaggatag gtcaaggcag ccactgctat
    36781 ccccacatag agaaccgtcc aacttggagt ggcatgaacc ctccgccctg aacaagcaaa
    36841 cttgatggta aaaccagcaa tcaaggtcaa atccaagaga aatgaaaacc accaaatccc
    36901 ttgtgctacc aaaggaagat aagagaatac gcgaaagaca taggtcgata aaatcatccc
    36961 agccatagga aaggttgcca ttcctgacaa aagagggggc ttggtcaatt cttgcttggt
    37021 ttctttccaa ttaaagagat gcagaattag aaagtaaatc cataaaacca aaccagtcag
    37081 actaaaaaga tgggatagaa ccggcaacgt atctaaaata agatttccag ctcctgccaa
    37141 acctagcaaa caacctgaaa atactaaggg gagttttttc atcctaacct ccaataatca
    37201 tgttagtttc agtataacat aaaagcgctt aaatgaggat ttaaaaaaac gaagtccgct
    37261 tatttcagac ttcattttac tcagatatga attaggcata aggttgcaat tctggattaa
    37321 ttggtgtatt agctaagttg ttggcatagt tacagaggat tgctaggctg acaccaaaaa
    37381 ccacatccaa ggcattttgt tgagtgtagc cagcttctaa aaactcagac aaggcttcat
    37441 ctcctacacg acccttggta ttgataactg ccaaggtaaa cttagctagg gtatccaatt
    37501 taggatctgt ttcaattgga gtacgattgc gaagagcttg aatcaagtca tcattcatct
    37561 ggatttgttt gatggaaaag gctgtgtgac ctgcgacaca gaaggcacaa ccattggtca
    37621 cggctgccgt gatttgcacc acttcacgct caacgggtgt caggctgttg cgacggtgga
    37681 tagatgagac aatttggtag gcttctaaaa cagtcggggc attggccaag agaccgatta
    37741 ggttgggaat atagccattg ttgtcttttt ctactgtttc aagaatttct ttcacttctg
    37801 ctggtgctga ctctactgta tggatagtaa atgttgtcat aagatacctc ttttcttatt
    37861 attgacacta atattattgg aaaatcttat aaaatcctga ttcctaagtt tatctaagat
    37921 aaagctttat tctctcataa gattttcgtt gttatattag tttatcacac ttccaatcac
    37981 ttgcataata tatattatat atcaggctga taaaaattat ttataggcaa aaaaatcaca
    38041 cgagctgtgt gattccatta tttgtcaaaa tactttttag tttcagcaat aacgactggc
    38101 gacaagacca agagggcaat caagtttggc agagccatca aggcgttaac gatatctgcg
    38161 ataatccaga ccatatccaa ctcgataaat cctcctaaca agaccatgag cacaaaaaac
    38221 acacggtaga gccagataaa gcgagcccca aagaggaact caaaacagcg ttctccgtaa
    38281 tagttccaac ctagaatcgt tgtaaaggta aaaagtacaa ggaagatggt caagagggca
    38341 ggcccaaagt gtgaaaagac tgttgagaaa gctgactgag tcaaggcaac cccattcaag
    38401 tcaccactcc aaactccagt taccaagatg gtcaaaccag ttagagtaca aatgatgagg
    38461 gtatcaataa aggttcctgt catggaaatc aaaccttgct ctactggttc atttgtcttg
    38521 gcagctgcag ctgcaatagg agcagaaccc agaccagatt cgtttgagaa cacaccacgc
    38581 gccacaccat tttgaatagc catccgaacg ctagcaccag caaatccacc taccgcagca
    38641 aggggactaa aagctgaggt aaagactaaa gcgattgtgc cagggatttt tccgatatta
    38701 aagaaaataa ctgtaagagt tcctaagata taaatgaggg ccataaaagg aacaacagta
    38761 gttgaaacct tagaaataga cttgagtcca ccaaagactg caatcgctac aaagacagac
    38821 aagacgagag ctgtgatggc tggcgaaatc gtcgttgtat tttggataga ttctgtaatc
    38881 gagttgactt gggtgaaggt tccgattccc aagagagcaa ccaatactcc tgctactgca
    38941 aacaaaacag caagtggtcg ccacttttct cccatcccta gaaggatata gtgcatggga
    39001 cctcccgcta ctgcaccatg gtcgtccttg gtgcggtatt tgatggccaa gagtccttcc
    39061 gcatacttgg tagccattcc aaagaaagcc gccatccaca tccaaaatag agctcctggt
    39121 ccaccaacct tgatagccgt cgcaactcct atgatatttc ctgttccaac agttgatgcc
    39181 aaggctgtac acagagctgc aaaactggat acatcaccat gtcccttatc ctggataaaa
    39241 ataagctgaa aggccttggg cagacgcaaa acctgcaaga gtcctagccg catggttagg
    39301 taaatccctg ttccgaccaa taaaatcaag aggggcggtc cccaagcaaa agcatcgatt
    39361 gatttaagca attctaacat tttcttctcc tatcgtttca accccaaaag aaagagcaca
    39421 tgcaagatac atgtactctg gaatgcttag ataaatgcta aaaagcggtc tatcctagct
    39481 ctgtcctttt acctgagagt ttgagcagtt gcctgccttg ccccttcggt gcctttacgg
    39541 tctctccaga gttccgtcca tttacagtca tggaaaatca aacgattccc cacttctatt
    39601 aaacttcatt cggtgttggt atttaattga ttctaatttt acaaaaaatg ttggcttttg
    39661 tcaatgtgtt tattagtaaa aattagttca acagttttta ctttataaag tccagaatac
    39721 tgctatcctt taaaagtgac aatagtcgca ccactgcctc cagcattttg tggggcatag
    39781 ccgaaactct tgacatgttt gtttctttgc aagtatttgg taactccttc acggatgact
    39841 cctgttccga taccatggat gatatcaact tgagccatat tgttaagcaa ggcttggtcg
    39901 atgaaggtat ctagctcatt catggcttct tcatagcgct tgcctcgaag atccagtcta
    39961 gcttgaggtc ctcgcccaga agttcgtttc acaacattga cctgtttctt cttgactggt
    40021 ttttcttgct gggcttgaac aagatcaaac tctttctctt ccaaggtcat cttaatcaag
    40081 ccaatttggg cttcccagcg gccgtccttg agttgactgg tcaaggtacc acgctgacca
    40141 taactgagaa ccacgatatc atctcccacc tttggagctc gttttttctt ggccttttga
    40201 aggaccttgt ttttagacaa gtccactttt tcaggagcca attttttcaa cttggccttg
    40261 gcttcaatga tttcgtgggg cttgagttgg gatttactgt ggagattttt gagaatctgg
    40321 tcactttcac ttagggccat atccacaatc tcagcagcct gttcacgcgc cttgttaagc
    40381 tcggtttcct tttcacgatt aagctcgttg tagagttttt ttagcgcacg gttcatcttg
    40441 agattttctt gctccacctc acggatattg tccaagcgtt tgcggctttc cagcgtctgc
    40501 tcttctaatt gctcaatgat acgattgacg tcattgtcct gatcgatctg ctgactggca
    40561 tctcctacga taacttcaga taggcctaga cgtttggcaa tttcaaaggc attacttcgg
    40621 ccaggaacac cctgcataaa gcgataggtc gggcgaagag ttgcagtatc aaactccata
    40681 ctggcatttt gcacaaaggc tgtctcaata ccgtaggcct tgagttctgg atagtgggtc
    40741 gtcgccatgg tcttgatttg acgcaggcga aggtcctcca gaatagccat ggcaagggct
    40801 gctccctctt ggggatcagt accagccccc aactcatcca aaagtaagag tgaatgttgg
    40861 ttgaccttgc caagaatatc cacgatattg gtcatatggc tagagaaggt agacaagctc
    40921 tgctcaatag actgctcatc tccaatatca gcaaagattt cttcaaaaat accaacacga
    40981 cttcccttgt ctgctaaaat cggcaatcct gactgggcca tgacctgtgt caagcccaga
    41041 gttttaagca tgatggtctt cccacctgta ttgggacctg taatgacaat agctgttaaa
    41101 tcttgaccaa aatagacatc atttgcgacg gcatttttga ccaaaggatg gcagacatgg
    41161 agcagttgaa tctcttgatt ttctgacagc tgaggcacga ctgcttgtct ttcttgaata
    41221 aatcgaacct tggcacgaat caagtccaga tgaccgataa tccaagcgtc attagcaatc
    41281 tcagccgcat gagggcggac acgctcagaa atttcttgga gaatgcgaag catttcatag
    41341 cgctcatctg ctcgcagact agcaatttct tcgctcagtt tgactacctc acgtggttcg
    41401 atatagacgg tgtttccact agcagaaata tcatgaacga cacctgcaat cttattgcgg
    41461 taggtgtttt tgactggtaa aacctgacgg ccatttctgc tagcaacaat tccttccgtc
    41521 aacatctgcc ctttttgctt gagcaagtct tgtaaaacat cgcgtacctg actctcgcta
    41581 tcatgtattt ttcgacggat tcgcgccaat tcttcactgg caaaattttc aatgaaaccc
    41641 gcatcattaa aggcctgaag atttccttgt aattgcggaa aatcatgtaa tttctcaaac
    41701 caaagggcta attcttccaa gctgacattt tccagattgg cgtaaaaact ttgaagttct
    41761 cggctggcaa gaagcacgcg tttcaagagt aggaactcct cgatattgag atccgctccc
    41821 atctccaacc tcttgcagac tcctgcaatt tccttagttg agagaatagt aaaatgcggt
    41881 tgctcgacga aaagagcctg catttctttc atctcagcaa aagcctgttt gattttatct
    41941 gctttggcag tcggagccag ctgtctcaat tgctccaagc cctgctcggt caacaaatga
    42001 ggctcaaaca aggccttgac cttatcgaac tctaatgttt ctaatatttt cttattcatc
    42061 tttatttcct tgtctttgaa tgtctaggtg tggtagcttt ttacattctg atagattcga
    42121 gtcaaatctg taaacaaagg gctaggaaaa ctcctgccct ttttatccga ttaaatttgt
    42181 cacccagatt tgtttgagcc aactggttgt tatcggtatg ctctggatga tgtgttttgc
    42241 gacgatactc ttttcaagag gattttgtat aactgccata gggatggtcg ccaagattgt
    42301 caaggccatt tgcaagacaa ataaggtcac caacatggac aagatacctg ctgaaacttg
    42361 gaacaactta ccacccagtt ttttgctagg aatcaagtgt aagagaagac caagcaaacg
    42421 accaatgcta tagacaatcc caaatacaag caagtagccg atacctgcat aaaagacctt
    42481 atccagctga aagagttgat ccgatgggaa aaagaaagtc ccctgacctt cctgcgaatt
    42541 tgcataaggg agcaataaat ggaattgctc tccaagcccc ttataaaact ggccagccat
    42601 aaaagccgat gccatggctg aaatcaggta ataaacctgt aagagcaggc ctctccgata
    42661 gccgatataa aatccccaaa ccaagaccaa tagaagaagg aatgaaatca taaggaatcc
    42721 tcaatcttgc tctgttcttg cttacaagtc acaagcttgt gacggagttc ttctagctct
    42781 tgctccttat cgtcaaattc aatctcacgg ctgagctgag ttgataaaca gttgactgcc
    42841 aacaaaagag cgattgtttc atcatctgcg ctaggcattt gttctttaat tgcttggtat
    42901 ttttctgtcg caaccttagc gatttcctcc ataaaaaggt tatcatgttc gcttgtcaag
    42961 gttaacgttt ttttcccgaa tgtaaatttg aatcgattta gatttgccat aaaattcacc
    43021 tcacgatatt ataccaaaat tcgctaattt tgtcagtttt tacaaatttt actgcttttg
    43081 tggtacaata gaaactatgg caagtatcac actcacacca agcgaaaagg atattcaggc
    43141 ttttcttgaa cactatcaaa ccagtctggc tcctagcaag aatccctata tccgctactt
    43201 tttgaaacta cctcaagcaa cggtttctat ctatacttct ggaaaaatct tgcttcaggg
    43261 tgaaggggct gaaaaatacg ccagtttctt tggctatcaa gctgtagagc aaaccagcgg
    43321 acaaaatctt cctttaattg ggacagatga ggtgggaaat ggttcctact ttggtgggct
    43381 tgcagttgtg gctgcctttg tcacacctga ccagcacgac tttttacgaa aactcggtgt
    43441 gggggattct aagactctga ccgaccaaaa gatccgtcag attgctccta ttctcaagga
    43501 aaaaattcag caccaggcac tccttctctc acccagcaag tacaacgagg tcatcggaga
    43561 ccgctacaat gctgtttcgg ttaaggttgc cctccataat caggctatct atctcctcct
    43621 tcaaaaaggt gttcagcctg agaaaattgt gattgatgcc tttaccagtg ctaaaaatta
    43681 tgacaagtac ttggcacaag aggccaatcg tttcagcaat cctatcagct tagaagaaaa
    43741 ggctgagggc aaatacttgg ctgtcgcagt ttcttctgtc attgcgcgtg atctctttct
    43801 ggaaaatctt gaaaatctgg gacgagaact gggttatcag ctcccaagtg gagctggaac
    43861 agcttctgac aaggtggcta gccagatttt gcaagcctat ggtatgcagg gactcagctt
    43921 ctgcgccaaa ttgcacttta aaaacactga aaaagcgaaa aaacgcttag aaaggtaagt
    43981 tatgaattca tttaaaaatt tcttaaaaga gtggggattg ttcctcctaa ttctgtcatt
    44041 actagcttta agtcgtatct ttttttggag caatgttcgc gtagaaggac attccatgga
    44101 tccgacccta gcggatggcg aaattctctt cgttgtaaaa caccttccta ttgaccgttt
    44161 tgatatcgtg gtggcccatg aggaagatgg caataaggac atcgtcaagc gcgtgattgg
    44221 aatgcctggc gacaccattc gttacgaaaa tgataaactc tacatcaatg acaaagaaac
    44281 ggacgagcct tatctagcag actatatcaa acgcttcaag gatgacaaac tccaaagcac
    44341 ttactcaggc aagggctttg aaggaaataa aggaactttc tttagaagta tcgctcaaaa
    44401 agcccaagcc ttcacagttg atgtcaacta caacaccaac tttagcttta ctgttccaga
    44461 aggagaatac cttctcctcg gagatgaccg cttggtttcg agcgacagcc gccacgtagg
    44521 taccttcaaa gcaaaagata tcacagggga agctaaattc cgcttctggc caatcacccg
    44581 tatcggaaca ttttaagaaa cctaagaggc cgagaatcac caatctcagc ctcttcttct
    44641 atcgtgagaa gtgattgttt attgacaaat actcaatgaa aatcaaagaa caaactagga
    44701 agctagccgt aggctgctta aaacagtgtt ttgaggttgc agatggaagc tgacgtggtt
    44761 tgaagatatt ttcgaagagt ataaaataaa aacaatccca actctcacct attcatgcaa
    44821 aggaatttta tggaagttta tttttcagga accattgaac ggattatttt tgaaaatccc
    44881 agcaattttt atcgcatcct cctcctagaa atcgacgata cggacgcaga ggattttgat
    44941 gactttgaaa tcattgtcac aggaaccatg gctgatgtaa ttgagggcga agactatact
    45001 ttttgggggc aaattgtcca gcactccaag tatggagaac aactgcaaat cagtcgttat
    45061 gatcgcgcaa aaccaactag taagggcttg gtcaagtact tttcaagtag ccatttcaag
    45121 gggattggtc tcaagacagc tcagaaaatc gtggatacct atggcgaaaa taccattgac
    45181 gaaattttgc aacacccaga aaagttagaa ggcatcgcag gactctctgc caaaaatcgc
    45241 gaggctttcg tctccactct ccgtctcaac tacggtacgg agatggtttt ggccaaacta
    45301 gccaactacg gcattcccaa caaactagcc tttcagattc aagactttta caaggaagaa
    45361 acccttgatg tggttgaaaa ttatccctac caactggttg aggatatcaa gggtttgggc
    45421 tttaccattg ctgaccaact agctgaggaa ctaggcatcg aaagtcaggc tcctgaacgc
    45481 ttccgcgccg gtctagttca cagtcttttt caggcctgta tggaaacagg agacacctat
    45541 gttgaagcac gggatttgct ggaacaaacc cttactctcc ttgagtcttc ccgtcccgtg
    45601 gaactagacc ccagccaagt tgcccaagag ctctcctacc tgatcgaaga agacaaggtt
    45661 cagcagattg ataccaagat ctttgacaac agcctctttt tcgctgagga aggcatccgc
    45721 agtcacttgg ttcgtatcct tgaaaaagga aaacagaaga gtcatgattt agaaaccatt
    45781 caaaaacata tcactactat cgaggaagag ctgggaattg agtatgataa cattcaaaaa
    45841 caggctatct gtgacgctat ccagaacaag gtctttatcc tgacaggtgg gcctggtact
    45901 ggtaagacaa ctgttatcaa tggaatcatc gctgtttatg cccttttaga aggacttgac
    45961 ttcaggaaga aaaacaatct gcccattctt cttgctgctc caactggacg agccgctcgc
    46021 cgcatgaatg aattgacagg tttgcctagc gcgaccatac accgccactt gggaatgaca
    46081 ggtgacgatg ataccagtca tctggaagat tacctagatg ctgactttat catcgtggat
    46141 gaattctcta tggtggatac ttggctggcc aatcaactct tctccaatat ctcttctaac
    46201 agtaagatcc tcatcgtggg tgacagtgat cagctacctt ctgtcagtcc tggacaggtt
    46261 ctagcggatc tgcttcatat tcctttgatt cctcagactc gcttggaaaa aatttatcga
    46321 caaagcgaag aatcaaccat cgtcacccta gctagtcaga ttcgacaggg catcttgcca
    46381 gctgatttca cccaaaaaaa agctgaccgt tcctactttg aaattgctag tggccatatt
    46441 cctgccacta ttgaaaaaat cttaggtgcc gccctcagaa atagtattcc tgcccgtgat
    46501 atccaagttc tggctcctat gtaccgaggg acggcaggga ttgatgctat caatcagctc
    46561 atgcaagacc tgctcaatcc cccacaaaaa gatcaactca gttttgaagc tcctcagtgc
    46621 cactatcgta agggagacaa ggtcattcat ttggttaacg atgctgaaat caatgtcttt
    46681 aatggggatt taggagctat cacagacctg attcctggta aatacaccga atcgaaacaa
    46741 gacgagattg tcattgattt tgatggtaat gaagtctctt acccccgtaa cgaatggtac
    46801 aagattcgct tggcctatgc catgagtatt cataagtctc agggaagtga gttcccagtt
    46861 gtcatcctac cgattactag tgctagccga cgtatgctgg agcgaaatct catctacaca
    46921 gccattacac gtgccaaaag caaactcatc ttactaggcg aattacaagc cttcgactat
    46981 gctacccaac acatcggaac tgcccgaaaa acctatctga ttgaacgctt cagtgatcta
    47041 ctggagaatg ttgaagaaaa gcaacaagct gtctctgaaa cagtcacatc aagtgcctct
    47101 gaacaatcct acatcctaac cgaagaaaac tgggacagca ttccagccat gattgggatt
    47161 acagacacag acctcaaaga gatttttgga aaatagtgca tcagaaaaac caccaattca
    47221 ggtgggtttt ctgtttatta agattatttt actgttgctg tgcttgtgat caattcaaca
    47281 gcttttttga tagtgatatc atgtttcaac atgtcagctg aaagcaagtt ttgaacttgt
    47341 gcaacttcca tgttgtagtc tgctgccaat tgctcaactt ctttttggat ttcttcttct
    47401 gaagcatcaa atccttcagc tttggcaact gcttcgataa caaggttagt cttagtacgt
    47461 gactcagctt ctgcttggta ttggttgtga aggtcttctt gagtagttcc agtgatttgg
    47521 aagtacatgt cagggttgat cccttgacgt tgcaaattcc caaggaattc atttactgaa
    47581 cggtgaactt cttcatggat catttcttct ggaagttcta cgatttcagc attttctaca
    47641 gctgtatcaa ttgctgcacc ttcaactgca tctttgtaag cttcttcttt agcagcagcc
    47701 aattctttgc ggtatttttc tttcaagtca gcaagtgttt caacttcttc atcaatgtct
    47761 tttgcaagtt catcgtcaag agctggaact tctttagctt ttacttcgtg gatagttgtc
    47821 acgaatttag cttctttacc tgcaaggtct tctgcttggt agtcttctgg gaatgttacg
    47881 ataacatcaa cggtttcgcc agctgagtga cctaccaatt ggtcttcgaa accagggatg
    47941 aattgacctg aaccaagtcc aagtgagaag ttttcacctt ttccaccgtc aaattcaaca
    48001 ccgtcgatag aaccaacgaa gtcgatcaca acagtgtcgc cgttttcagc agcagcttcc
    48061 ttgataacca attcagccag gttgttgcgt tcgcgttcga tacgctcttc gacatcagcg
    48121 tcagttactt ctttttctac atcaactgat acttcaaggt ttttgtagtc acccaatttt
    48181 acttcaggtt ttgtaacgac ttcagcagtg ataacccagt cttgaccttt ttccattgaa
    48241 gttacgtcaa tttttggttg ggcaaccact tcaagaccag cttcttttac agctgcttca
    48301 taagcgtttg gcaaaagagc gttcataacg tcttggtaaa gtgattcttc accaaatttt
    48361 tggtcgaaga taggacgtgg aaggtgacct ttacggaaac ctggaacatt aagagatttc
    48421 ttcactgact tgaagacacg gtccaattct ggtttgattt ggtcttgaga gatagtgaaa
    48481 gtcaagacac cacggtttgt ttctttgttt tcaaatgata cagacattct gtcatttctc
    48541 cttaaaattt tttaaataca gtctattata acattttcta taaataaaat ctagactaat
    48601 gcagccaatt atagacaaca agcctttttc ctaactaaaa cactcataaa gtatagacaa
    48661 ttttagacta ctttggatat cttttggtta gtcattttcc aaaactaatt tatctaattt
    48721 ttcagttata tagta
//
LOCUS       DAVKFH010000010        44729 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKFH010000010 DAVKFH010000000
VERSION     DAVKFH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.010"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(15..824)
                     /locus_tag="VW006_000600"
     CDS             complement(15..824)
                     /locus_tag="VW006_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9379500.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(826..1629)
                     /locus_tag="VW006_000601"
     CDS             complement(826..1629)
                     /locus_tag="VW006_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9379501.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDNLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1639..3126)
                     /gene="tacF"
                     /locus_tag="VW006_000602"
     CDS             complement(1639..3126)
                     /gene="tacF"
                     /locus_tag="VW006_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9379502.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3462..4169
                     /locus_tag="VW006_000603"
     CDS             3462..4169
                     /locus_tag="VW006_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9379503.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4174..5196
                     /locus_tag="VW006_000604"
     CDS             4174..5196
                     /locus_tag="VW006_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9379504.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5213..6082
                     /gene="licA"
                     /locus_tag="VW006_000605"
     CDS             5213..6082
                     /gene="licA"
                     /locus_tag="VW006_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9379505.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6072..6950
                     /locus_tag="VW006_000606"
     CDS             6072..6950
                     /locus_tag="VW006_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9379506.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            6962..7651
                     /locus_tag="VW006_000607"
     CDS             6962..7651
                     /locus_tag="VW006_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9379507.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7747..8595
                     /gene="dprA"
                     /locus_tag="VW006_000608"
     CDS             7747..8595
                     /gene="dprA"
                     /locus_tag="VW006_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9379508.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9484..10527
                     /locus_tag="VW006_000609"
     CDS             9484..10527
                     /locus_tag="VW006_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9379509.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10694..12781
                     /gene="topA"
                     /locus_tag="VW006_000610"
     CDS             10694..12781
                     /gene="topA"
                     /locus_tag="VW006_000610"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9379510.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            12887..13246
                     /locus_tag="VW006_000611"
     CDS             12887..13246
                     /locus_tag="VW006_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9379511.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13339..13971
                     /locus_tag="VW006_000612"
     CDS             13339..13971
                     /locus_tag="VW006_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9379512.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            14015..14671
                     /locus_tag="VW006_000613"
     CDS             14015..14671
                     /locus_tag="VW006_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9379513.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14690..15322
                     /locus_tag="VW006_000614"
     CDS             14690..15322
                     /locus_tag="VW006_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9379514.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            15350..15784
                     /locus_tag="VW006_000615"
     CDS             15350..15784
                     /locus_tag="VW006_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9379515.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15796..16833
                     /gene="leuB"
                     /locus_tag="VW006_000616"
                     /pseudo
     CDS             15796..16833
                     /gene="leuB"
                     /locus_tag="VW006_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            16830..17099
                     /locus_tag="VW006_000617"
     CDS             16830..17099
                     /locus_tag="VW006_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9379516.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17103..17462
                     /gene="leuD"
                     /locus_tag="VW006_000618"
     CDS             17103..17462
                     /gene="leuD"
                     /locus_tag="VW006_000618"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9379517.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17624..18247
                     /locus_tag="VW006_000619"
                     /pseudo
     CDS             17624..18247
                     /locus_tag="VW006_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            18413..20344
                     /locus_tag="VW006_000620"
     CDS             18413..20344
                     /locus_tag="VW006_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9379518.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSGVSGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            20331..21647
                     /locus_tag="VW006_000621"
     CDS             20331..21647
                     /locus_tag="VW006_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9379519.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFFHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            21822..22808
                     /locus_tag="VW006_000622"
     CDS             21822..22808
                     /locus_tag="VW006_000622"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9379520.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCVKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            22872..22943
                     /locus_tag="VW006_000623"
     tRNA            22872..22943
                     /locus_tag="VW006_000623"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22905..22907,aa:Arg,seq:ccg)
     gene            23126..23824
                     /locus_tag="VW006_000624"
     CDS             23126..23824
                     /locus_tag="VW006_000624"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9379521.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            23815..27354
                     /gene="smc"
                     /locus_tag="VW006_000625"
     CDS             23815..27354
                     /gene="smc"
                     /locus_tag="VW006_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9379522.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSHQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            27351..28145
                     /locus_tag="VW006_000626"
     CDS             27351..28145
                     /locus_tag="VW006_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9379523.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            28145..28963
                     /locus_tag="VW006_000627"
     CDS             28145..28963
                     /locus_tag="VW006_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9379524.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            28967..30244
                     /gene="ftsY"
                     /locus_tag="VW006_000628"
     CDS             28967..30244
                     /gene="ftsY"
                     /locus_tag="VW006_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9379525.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVIEVSQKEVKEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(30290..31777)
                     /locus_tag="VW006_000629"
     CDS             complement(30290..31777)
                     /locus_tag="VW006_000629"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9379526.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWD
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(31926..32666)
                     /locus_tag="VW006_000630"
     CDS             complement(31926..32666)
                     /locus_tag="VW006_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9379527.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(32666..34831)
                     /locus_tag="VW006_000631"
     CDS             complement(32666..34831)
                     /locus_tag="VW006_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9379528.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            35004..35957
                     /locus_tag="VW006_000632"
     CDS             35004..35957
                     /locus_tag="VW006_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9379529.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLQQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSSLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            36034..38022
                     /gene="uvrB"
                     /locus_tag="VW006_000633"
     CDS             36034..38022
                     /gene="uvrB"
                     /locus_tag="VW006_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9379530.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            38032..38595
                     /locus_tag="VW006_000634"
     CDS             38032..38595
                     /locus_tag="VW006_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379531.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            38561..39049
                     /locus_tag="VW006_000635"
     CDS             38561..39049
                     /locus_tag="VW006_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379532.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQMWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAIGDLKVVGFISYGNFRDETMQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            39123..39590
                     /locus_tag="VW006_000636"
     CDS             39123..39590
                     /locus_tag="VW006_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9379533.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(39587..40102)
                     /locus_tag="VW006_000637"
     CDS             complement(39587..40102)
                     /locus_tag="VW006_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9379534.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(40114..40641)
                     /locus_tag="VW006_000638"
     CDS             complement(40114..40641)
                     /locus_tag="VW006_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9379535.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(40919..41482)
                     /locus_tag="VW006_000639"
     CDS             complement(40919..41482)
                     /locus_tag="VW006_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9379536.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(41466..42017)
                     /gene="coaC"
                     /locus_tag="VW006_000640"
     CDS             complement(41466..42017)
                     /gene="coaC"
                     /locus_tag="VW006_000640"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9379537.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(42029..42718)
                     /gene="coaB"
                     /locus_tag="VW006_000641"
     CDS             complement(42029..42718)
                     /gene="coaB"
                     /locus_tag="VW006_000641"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9379538.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            42948..44618
                     /locus_tag="VW006_000642"
     CDS             42948..44618
                     /locus_tag="VW006_000642"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9379539.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIYVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT        13878 a         7851 c         9735 g        13265 t
ORIGIN      
        1 ataatccccc tcaatcagtt tttgcgataa gctttaatgc tatgactata ccactcttgc
       61 atttcttttg atggtggtgt catataatcg ccatacatct gggttaaaaa ttggtcatat
      121 tttttgggaa caggcaacat acggccctca aactcagtta aaatcagttc tttaaaggta
      181 tcaactggga agatttcttt catcccttcc ttaccgatcc caactcctcc ttcatattga
      241 ggaatgttgg ttacagcatt tttgactagt tgatcaattt tcttgtaaaa gtagcgagga
      301 ttgacaaatc ggagagcgta ccagctacat aatctaagaa aatcttttag tttgctatca
      361 ccgtgaactg ctcgtgattt tttgatataa gctagttgac gaagagccac atacttatag
      421 ctcttgtcga caatgctcaa gtctgtaaat cgatcaattg ggaagacatc gatgaaaagg
      481 ctggtatcat gacgcttgta cttaacatgg tcttctataa cagtagaagt gtccaaaatc
      541 gatgcgaaat tatggaagta ccaagaagat gtatcgtagg aaagaacctt gtagcgaggg
      601 tgattttctt cttcaataat cttcagtaaa cgctcataat cctcacgata aagggaaata
      661 tcaatatcat catcccaagg aatcatacct ttgtggcgga tggctccaag catggttcca
      721 taactgagaa aataaggaat atcatgtttc ttacaagtct catcaatata gtccagcagg
      781 gctagttgaa tttctttaat ttcttttttt tctaaatatt gcatcctaat cctccaattt
      841 ataagcgtga aattcatgac tgtagaagcg tttttcttct ggtggtaggg tcatataatc
      901 tccataaaat tgtgtcaaaa tagtatcaaa tttttcaggt gcaggaaggc ttaaattctc
      961 aaagggtaaa tcgattgttt tatcaaaggt accacttggg aagacttcct tttccttaaa
     1021 ttttgaaggg ataaaagcca tatactgccc attttcacga ctatattttt gaatttcttt
     1081 ctcgatttta tttgcaaaat aacgaggaga aactggtcga aggagcaacc aaaaggctgt
     1141 tcgtatccaa tcttttaaaa ggttatcctt atagacaata tttttatgtt tactgaaaga
     1201 cagcagtttg aagctttcca gtttataaca agtatcaatg accttaggat catcaaagcg
     1261 atccataggg aaaatatcga taaagatacc agactcatag gtttttgtat ttcgagtatc
     1321 aataatttta gtcgtactgt cggttatctt gataaagttg ttaaagtagt tcttatcagt
     1381 ttctaaggat aggagttcat acttgctttt ttccttttga aaaatgttaa taaatcgttg
     1441 gtagtcttct ctaggcatgg ataaatcaat atcgtcgtcc caagggataa agccctcatg
     1501 tcgaaccgcc ccaatcagag taccgtagtt aataatatag ttgatattgt gctttttaca
     1561 gagagtatca atataatcca aaatttctaa ttcaatttgt ttggcatctt caacggttag
     1621 ttgtttcatt ttaaactcct atgatttttt aaatttattt tttaaggcta gaacatggtt
     1681 taaaaattca tagaaaatgc tatcttttgt gaagacaagt agactaatat aagagatagc
     1741 tgatagcaag ataatcaaac cagtattaat caaaaatggc aaattaatga ccatatccac
     1801 aggatacacg aaattaatca ggaaataaat tgctacaaag gaaagtgaaa agagagagta
     1861 tcgaacagta tagctaaaga tatgtcccaa gtggatgagt tgttttctat ggatgaaaat
     1921 gatatagaaa acaagtagag aagcctctga taacatagtt gtcagtaagt agtattcggg
     1981 agccacgata cggttgaaaa agaggagact attcaagccc aaattgagta gcccagcaaa
     2041 gactgtataa actgtgatac gtttttcata gccattggta aagagaattt gggaaccaag
     2101 aatggtatct aaggccagga taatcgtacg aaaagcgaag agagaggtca agatgccgcc
     2161 tccgatatat ttttcactac cgtaaagtag gatggcattt ggtcctaaaa ccatgagtcc
     2221 aaaactcagt ggaatgataa agaagttaaa gattcgacta cctctattaa ccagagaaac
     2281 ataggcttct ttgtctcctt tccccagata gtaactgaga cgaggcacac tcactccaat
     2341 tgcacctgtt acaaccccag ctataacggt cacaattcgc tgagctatgg tatagtaact
     2401 aacgttgaca tcaatccctg ttttaacgag gaagaggcga tctaaaaaag tgaagagcat
     2461 attggcattg gcaaagacta acatggctgt cagagggaga aagagtggtt taaaatcact
     2521 taggtgaatc ttaacaagtt tgatgtctct tttaatccaa aaataactaa tcaggtagtt
     2581 aatcagcgtc gataaactca tcacaagtgt atagacaaca atatcgtgtt catttttaac
     2641 aaataagaaa atagagacca gcatcaggat acggatgaag gcagttttgt aaaagagaaa
     2701 actgtaattt tccagagctt cattgaccca ttcgattgaa aaaatctggg caatgagttg
     2761 aatccccata acaaggtaga cctttttgat gattggatta tcagtaaaga agagaggata
     2821 ggctaggata tagacagcag tggtcaaaat cgtacaagcg atgcacaaat aaaaaagact
     2881 agaaaaggtt ctgttaagat cttttttgtt atccttgaca ttactgatag cccttaaacc
     2941 gtagttatag acaccataag ttgcaaaggg caagaaaaat gacaaaatag tgtcgactga
     3001 gttgaagtaa ccatagtcag ttcggtccaa gacacgcgcg acataggttc cagttaggat
     3061 gggaaaaata atattcaaga cacgaattcc catgtaagat agagcattta attttatact
     3121 tttcattcaa tttacctcgt ttttcattat atcataaagt tagctaataa gaaatgaagg
     3181 gcagtaagcc aagcaatcac tttgaagttt caaatcttaa attttaagtt ttctttaagg
     3241 aaagtatatt attctgaagg actctaaaat ttcgcagtca tttattagta attgctacag
     3301 aattcctagt cattactaga aatggactag tttctttgaa taatagaact gcataattct
     3361 cctattctag aaggggagga ccagtatttc ttttatgata ggactagatt gtggtataat
     3421 agagagaata agttttttta gtaagacaaa ggagaaaata gatgatttat gcaggaattc
     3481 ttgccggtgg aactggcaca cgcatgggga tcagtaactt gccaaaacaa tttttagagc
     3541 taggtgatcg acctattttg attcatacaa ttgaaaaatt tgtcttggag ccaagtattg
     3601 aaaaaattgt agttggtgtt catggagact gggtttctca tgcagaagat cttgtagata
     3661 aatatcttcc tctttataag gaacgtatca tcattacaaa gggtggtgct gaccgcaata
     3721 caagtattaa gaaaatcatt gaagccattg atgcttatcg tccgcttact ccagaggata
     3781 tcgttgttac ccacgattct gttcgtccat ttattacact tcgcatgatt caggacaata
     3841 tccaacttgc ccaaaatcat gacgcagtgg acacagtggt agaagcagtt gatactatcg
     3901 ttgaaagtac caatggtcaa tttattacag atattccaaa tcgtgctcac ctttatcaag
     3961 gacaaacacc tcaaacattc cgttgcaagg acttcatgga cctttatgga tctctttctg
     4021 atgaagagaa ggaaatcttg acagatgcat gtaaaatctt tgtgatcaaa ggaaaagatg
     4081 tggccttggc caaaggtgaa tactcaaatc tgaagattac aaccgtaaca gatttgaaga
     4141 ttgcaaaaag tatgattgag aaagactagt aaaatgatta atcaaattta tcaactaact
     4201 aagcctaagt ttatcaatgt caaatatcag gaagaggcta ttgaccaaga gaatcatatc
     4261 cttatccgtc ccaactacat ggctgtctgt catgcggatc agcgttacta tcagggaaaa
     4321 cgtgatccca agattttgaa taaaaagctt ccaatggcaa tgattcacga gtcatgtgga
     4381 accgtcattt ctgacccgac cggaacctac gaggttggtc aaaaagttgt catgattccc
     4441 aatcagtctc ctatgcagag tgatgaagaa ttctatgaaa actacatgac agggacccat
     4501 ttcttgtcta gtggatttga tggctttatg agagagtttg tttctctccc taaagatcgt
     4561 gtggtggctt atgatgctat tgaagatacg gttgcagcca ttacagagtt tgtcagtgtg
     4621 ggcatgcacg ctatgaatcg tctattgact cttgctcata gcaagcggga gcggatcgcc
     4681 gttattggag atggaagttt agcttttgtg gttgccaata ttatcaacta tactttgcca
     4741 gaatcagaga ttgtggttat tggtcgtcat tgggaaaagt tggaactctt ctcatttgcc
     4801 aaagaatgct atattacgga taatattcct gaagagttgg cctttgacca tgcttttgaa
     4861 tgttgtggtg gtgatggtac tggaccagct attaatgact tgattcgcta cattcgtcct
     4921 cagggaacga ttctcatgat gggagttagc gaatataaag tcaatctcaa tactcgcgat
     4981 gccttagaaa agggcttgat tttggttggg tcatctcgtt ctggtcgcat tgattttaaa
     5041 aatgctatcc aaatgatgga agtcaagaaa tttgccaatc gtcttaaaaa tatcctttat
     5101 ctagaagaac ctgtaagaga aattaaagat attcatcgtg tctttgcaac cgatttaaac
     5161 acagccttta aaacagtgtt taagtgggaa gtataagtac tggaggttaa ttgtggagaa
     5221 aatcattaaa gaaaaaattt cttccttact tagtcaagaa gaggaagtcc tcagtgttga
     5281 acaactgggt ggaatgacca atcaaaacta tttggccaaa acaacaaata agcaatacat
     5341 tgttaaattc tttggtaaag ggacagaaaa gcttatcaat cgacaagatg aaaagtacaa
     5401 tcttgaacta ctaaaggatt taggcttaga tgtaaaaaat tatctttttg atattgaagc
     5461 tggtatcaaa gtaaatgagt atatcgaatc tgcgattacg cttgattcaa cgtcaatcaa
     5521 gaccaagttc gacaaaattg ctccaatatt acaaactatt catacgtctg ctaaggaatt
     5581 aagaggagaa tttgctcctt ttgaagaaat caaaaaatac gaatccttga ttgaagaaca
     5641 aattccttat gccaactatg aatctgttag aaatgcagtc ttctccttag agaaaagact
     5701 ggctgactta ggtgttgaca gaaaatcttg tcatatcgat ttggtgcctg aaaactttat
     5761 cgaatcacct caaggacgac tttatttgat tgactgggaa tattcatcaa tgaatgatcc
     5821 aatgtgggat ttggctgccc tctttttaga gtctgaattc acttcccaag aggaagaaac
     5881 tttcttatct cactatgaga gtgaccaaac accggtttct catgaaaaga ttgctattta
     5941 taaaatttta caagatacta tttggagtct atggactgtc tataaggaag agcaaggtga
     6001 agattttggt gactatggtg tgaatcgtta ccaaagagct gttaaaggtt tggcttctta
     6061 tggaggttca gatgaaaagt aaaaacggag ttccttttgg ccttctctca ggtattttct
     6121 ggggcttggg tctaacggtt agtgcttata tcttttcgat ttttacagat ttgtcaccct
     6181 ttgtggtggc tgcagctcat gattttttga gcatctttat cttactagct tttctcttgg
     6241 taaaagaagg gaaagttcgc ctctcaattt tcttaaatat tcgcaatgtc agtgttatca
     6301 tcggagcctt gctagcaggc cctatcggta tgcaggccaa tctttatgca gttaagtata
     6361 tcggaagttc tttagcttca tctgtatcgg ctatttaccc tgcgatttca gttctattgg
     6421 ctttcttctt tttgaagcac aagatttcga aaaatactgt atttgggatt gtcttgatta
     6481 ttggagggat tattgctcag acctataagg ttgaacaggt taattctttc tacattggga
     6541 ttctttgtgc tttggtttgt gctattgcat ggggaagtga gagtgttctt agctcttttg
     6601 ccatggaaag tgaattgagt gaaatcgaag ccctcttaat ccgtcaagta acttcgttct
     6661 tgtcctatct tgtgattgtg ctcttctctc atcagtcatt tactgcagta gccaatggac
     6721 aattgctagg tctcatgatt gtttttgcag cctttgatat gatttcctac ttggcttatt
     6781 atatcgctat caatcgcttg caaccagcca aggctacagg cttgaacgtg agctatgtag
     6841 tatggacggt cttgtttgca gttgttttct tgggtgcacc gctagatatg ctgaccatta
     6901 tgacgtcact tgtcgtcatt gctggagttt atattattat taaagaataa aggagattcg
     6961 tatgaaagcc attatcttag cagcgggatt gggaactcgc ttgcgtccta tgactgaaaa
     7021 tacccctaaa gccttggttc aggttaatca aaaacctttg attgagtacc aaattgagtt
     7081 tctcaaagaa aaaggaatca atgacatcat catcattgtt ggttatctta aagaacaatt
     7141 cgattacttg aaagagaaat acggtgttcg tctcgttttc aatgataaat acgctgacta
     7201 caataacttt tactctctct atcttgtaaa agaagaattg gccaacagct atgttattga
     7261 tgctgacaat tatctcttta aaaatatgtt ccgcaatgat ttgacacgtt cgacttattt
     7321 tagtgtttat cgtgaagatt gtaccaacga atggttcttg gtttatggag atgactacaa
     7381 ggttcaagac attattgttg atagcaaggc aggtcgcatc cttagtggtg tatccttctg
     7441 ggatgctcca actgcagaaa agattgtcag ctttatcgac aaggcttatg taagtggtga
     7501 atttgttgat ctctattggg acaatatggt taaggataat atcaaagagc tagatgtcta
     7561 tgttgaagaa ttagaaggca atagcattta tgagatcgat agtgtccaag actatcgtaa
     7621 attagaagaa attcttaaaa acgaaaatta aagattccaa catctgacaa aatagtcgga
     7681 tgttttttga ttttttacga acttttacga atagatagat gagtagaaaa agaaatggag
     7741 ttatttatga aaatcacaaa ctatgaaatc tataagttaa aaaaatcagg tttgaccaat
     7801 caacagattt tgaaagtgct agaatacggt gaaaatgttg atcaggagct tttgttgggt
     7861 gatattgcag atatctcagg ttgccgtaat ccagccgttt ttatggaacg ttattttcag
     7921 atagacgatg cgcatttgtc gaaagagttt caaaaatttc catctttctc tattttagat
     7981 gactgttatc cttgggattt gagtgaaata tatgatgcgc ctgtactttt attttacaag
     8041 ggaaatcttg acctcctgaa attcccgaag gtagcggtcg tgggcagtcg tgcttgtagc
     8101 aaacagggag ctaagtcagt tgaaaaagtc attcaaggct tggaaaatga actggttatt
     8161 gtcagtggtc tggccaaggg cattgacaca gcagctcata tggcagctct tcagaatggc
     8221 ggaaaaacca ttgcagtgat tggaacagga ctggatgtgt tttatcctaa agccaataaa
     8281 cgcttgcaag actacatcgg caatgaccat ctggttctaa gtgaatatgg acctggtgaa
     8341 caacctctga aatttcattt tcctgcccgt aatcgcatca ttgctggact ttgtcgtggt
     8401 gtgattgtag cagaggctaa gatgcgttca ggtagtctca ttacgtgtga gcgagcaatg
     8461 gaagaaggac gcgatgtctt tgctattcct ggtagcattt tagatggact atcagacggt
     8521 tgccatcatt tgattcaaga aggagcaaaa ttagtcacca gtgggcaaga tgttcttgcg
     8581 gaatttgaat tttaaaaatg acctaagcta gaattctaag aaaaaatcaa ttttaagaga
     8641 aaatgaaccc aacatttcca taataaaacg catattagca agtttttaac acttgataat
     8701 atgcgttttt tctaagtgga ttagtagagt agaggatttt tctcatataa tactcttcga
     8761 aaatctcttc aaactacgtc agcttccatc tgcaacctca aaacagtatt ttgagctgac
     8821 ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag caacctgtgg ctagcttcct
     8881 agtttgcgct ttgattttca ttgagtataa gggaaagtat agtgaattga aataagatgt
     8941 gaacaactct atcaggaaag tcaaattgat ttatagaaat attttagcag ccaaggtgca
     9001 ctgttataga ttcaatacac tataatttag tgtaattgag aaaggagaaa tgattgtgat
     9061 tgatgttggc taggttatgt tcaatgattc ctaccgtctc aaatcttgtc agtaaggaaa
     9121 aataaattct tcaaaagtag agattacaag gcttgtttaa gaaagaattc aaagaccttg
     9181 acaaataaaa ataaaatggt tattataaaa aatggtctga aatagatgat gatacttttc
     9241 gaaaatctct tcaaactacg tcagctcagc tttgccttgc tgtgttttga gcaagctacg
     9301 gttagcttcc gagtttgatt ttcatttact agaaatgaaa ctgatgagag atatcagtag
     9361 acatttgagt caggatatta tggaaaatga taaaaagagc tcgtgagatt ggcatatcag
     9421 actactaaag tattgagttt gttaggattt tagcgactag ttagctggga aaggaagata
     9481 tttgtgacaa ataataaact gtattcgttg atagaattta gaaataaaat atatgaagaa
     9541 ttagaacttt ccagaagtga tttagcgatt ttactatgtg ccatgcttat cgcctctatc
     9601 ggattaaata tggattcgac tcccgtgatt attggagcca tgttaatctc tcctttgatg
     9661 acacctattc tgggagtggg gctctctcta gctatatttg attttaaatt gttaagaaaa
     9721 tcttttaaaa tattagctat tcaaattctt gccagtctaa tagcttcaac actttatttt
     9781 tatctttctc ccatttcgta tgctagttcg gagattgttg ctagaacctc tccgactatt
     9841 tgggatgttc tcattgcttt tgtaggaggg atagcaggta ttattggtgc taggaaaaaa
     9901 gagaccaata atattgttcc tggtgttgct attgcaaccg ccttgatgcc tcctctttgt
     9961 acagtaggtt atgctattgc ttctgctaat ctaaaattta tcataggctc ctcttaccta
    10021 ttcctcatca attgtagctt tattgtcatt gcgacttata taggtgttag gttgatgatg
    10081 gttaagaaac attattttaa agataatgaa gaagactcta aaatgcgtag gattttgctt
    10141 ctagttgctg ttttgctgat gattccgagt ttcatctctg caacgacttt agtgagagaa
    10201 acgttgaaaa aagagtccct taagaaattt atatcagagc agtttcaggg gtataatatt
    10261 ttgaaaaaaa cctattctaa aaagactcat accctaaagc taaccatttc aggaaattat
    10321 ttgacagaag aagaactcga tatgatttca agtaagagag gtgactatgg tttaagtgat
    10381 gtttctgttc aagtttcaca attgtctgat tcagaacaac ttagtaagga agagctggtg
    10441 gagtatttct tccagtatat caaggataag gaagcaaaag aaaaggagaa agccaataaa
    10501 tttgatacag agtctgagga gcaataattt cttgagaata gctggtttct ctcgtgagtc
    10561 ttctatgtat atcaaaggaa gactgaggtt ttaaatataa aacttttctt ctattatagt
    10621 agctaagtct tgacagggtt caaaaaatgg tttacacttt ataaagttta ttactttgaa
    10681 aaggtgtgat actgtggcta cggcaacaaa aaagaaaaaa tcaacagtta aaaaaaatct
    10741 agtcatcgtg gagtcgcctg ctaaggccaa gacgattgaa aaatatctag gcagaaacta
    10801 caaggtttta gccagtgtcg ggcatatccg tgatttgaag aaatccagta tgtccgtcga
    10861 tattgaaaat aattatgaac cgcaatatat taatatccga ggaaaaggcc ctcttatcaa
    10921 tgacttgaaa aaagaagcta aaaaagctaa taaagttttt ctcgcgagtg acccggaccg
    10981 tgaaggagaa gcgatttctt ggcatttggc ccatattctc aacttggatg aaaatgatgc
    11041 caaccgtgtg gtcttcaatg aaatcaccaa ggatgcagtc aaaaatgctt ttaaagaacc
    11101 tcgtaagatc gatatggact tggtcgatgc ccaacaagct cgtcggatct tggatcgctt
    11161 ggtagggtat tcgatttcgc ctattttgtg gaagaaggtc aagaagggct tgtcagcagg
    11221 tcgcgttcag tccattgccc ttaaactcat cattgaccgt gaaaatgaaa tcaatgcctt
    11281 ccagccagaa gaatactgga cagttgatgc tgtctttaaa aagggaacca aacaatttca
    11341 tgcttccttc tatggagtag atggtaaaaa gatgaaactg accagcaata acgaagtcaa
    11401 ggaagtcttg tctcgtctga cgagtaaaga cttttcagta gatcaggtgg ataagaaaga
    11461 gcgcaagcgc aatgctcctt taccctatac cacttcatct atgcagatgg atgctgccaa
    11521 taaaatcaat ttccgtactc gaaaaaccat gatggttgcc caacagctct atgaaggaat
    11581 taatatcggt tctggtgttc aaggtttgat tacctatatg cgtaccgatt cgactcgtat
    11641 cagtcctgta gcgcaaaatg aggcggcaag cttcattacg gatcgttttg gtagcaagta
    11701 ttctaagcac ggtagcaagg tcaaaaacgc atcaggtgct caggatgccc atgaggctat
    11761 tcgtccgtca agtgtcttta atacaccaga aagcatcgct aagtatctgg acaaggatca
    11821 gcttaagcta tataccctta tctggaatcg ttttgtggct agccagatga cagcggccgt
    11881 ttttgatacc atggctgtta aattgtctca aaaaggggtt caatttgctg ccaatggtag
    11941 tcaggttaag tttgatggtt atcttgccat ttataatgat tctgacaaga ataagatgtt
    12001 accggacatg gttgttggag atgtggtcaa acaggtcaat agcaaaccag agcaacattt
    12061 cacccaaccg cctgcccgtt attctgaagc aacactgatt aaaaccttag aggaaaatgg
    12121 ggttggacgt ccatcaacct atgcgccaac cattgaaacc attcagaaac gttattatgt
    12181 tcgcctggca gccaaacgtt ttgaaccgac agagttggga gaaattgtca ataagctcat
    12241 cgttgaatat ttcccagata tcgtaaacgt gaccttcaca gctgaaatgg aaggtaaact
    12301 ggatgatgtc gaagttggaa aagagcagtg gcgacgggtc attgatgcct tttacaaacc
    12361 attctctaaa gaagttgcca aggctgaaga agaaatggaa aaaatccaga ttaaggatga
    12421 accagctgga tttgactgtg aagtgtgtgg cagtccaatg gtcattaaac ttggtcgttt
    12481 tggtaaattc tacgcttgta gcaatttccc agattgccgt catacccaag caatcgtgaa
    12541 agagattggt gttgagtgtc caagctgtca tcagggacaa attattgagc gaaaaaccaa
    12601 gcgtaatcgc ctcttctatg gttgcaatcg ctatccagaa tgtgaattta cctcttggga
    12661 caagcctgtt ggtcgtgact gtccaaaatg tggcaacttc ctcatggaga aaaaagtccg
    12721 tggtggtggc aagcaggttg tttgtagcaa aggcgactac gaggaagaaa agattaaata
    12781 agaggagggg cccgaaagta aatttcaggc tccttttggt tagaacttga caaaatacat
    12841 cattttatgc gaaactagaa gaagattatt ttaactagga gaagttatgc gtcttatcta
    12901 tctgataatt ggttttttat cactggcctt ggctattgtt ggggttgttt tacccttgtt
    12961 gcctacaaca cctttccttt tgttgtctat tgcttgtttc tccagaagtt ccaagcgatt
    13021 cgaagactgg ctttatcata ccaagctcta tcaaacatat gtagctgatt ttcgcgagac
    13081 taagtctatt acgcgtgaac gaaagaaaaa aatcatcgta tccatttaca ttttgatggg
    13141 gatttctatt tattttgccc ctctcttacc agtcaaaatc ggtctgggtg ctttgaccat
    13201 ctttatcact tattatctct tcaaggtcat tccagacaaa gaatagttaa aacagtagtt
    13261 atttaccttg ataaaattga aagcatattc ataacaatat gatataataa atttgaagta
    13321 atattcaagg agaatcaaat gatttacgaa ttttgtgctg aaaatgtgac tttacttgaa
    13381 aaagcgatgc aggctggagc tcgtcggatt gaactctgtg ataatctagc agttggtggg
    13441 acaacaccta gctatggagt gactaaggca gcggttgaac tggcagctaa ctacgataca
    13501 accatcatga ccatgattcg gccacgtggt ggtgactttg tctataatga cctagaaatt
    13561 gctatcatgc tagaagacat ttgtttgact gctcaggctg gaagtcaagg ggttgtattt
    13621 ggagctttaa ctgctgataa aaagttggat aagcctaatc tggaaaagtt aattgctgca
    13681 tcaaaaggaa tggaaattgt ctttcacatg gcctttgatg aactaagtga tgaagatcaa
    13741 ccggaagcta ttgactggct cagtcaagcc ggtgtcactc gtatcctaac tcgtgctggt
    13801 gtgtctggcg actccttaga aaaacgtttt gttcactatc acagaatttt ggagtacgct
    13861 aaaggtaaaa ttgaaattct accaggtggg gggattgacc ttgacaaccg tcaaaccttt
    13921 attgaccagg tgggggtaac acaattgcat ggtactaagg ttgtttttta aaaaatagaa
    13981 aggaactgct agccttgggt agcagttttc acttatgttt gaaattttta aatcctatca
    14041 atttaatcaa gaaaaggctc atgattatgg ttttatagaa aatagcgaag tctggacata
    14101 tagttgccag attttgcaag gtgactttgt catgactgtg tccatcactg ctgataatgt
    14161 gaactttcaa gtctttgacc aagagactgg tgacctctat cctcacgttc atatggaaag
    14221 catgagggga agttttgtcg gaaatgtccg tgaggcttgt ctggagattc tttaccagat
    14281 tcggaaggct tgttttgatg tgcaagattt tatctgtcat cagactaagc gtatcatgac
    14341 tcaagttcag gaaaagtatg gaaaccagtt ggagtatctg tgggaaaaat cgcctgatac
    14401 agctgtattg cgccatgaag gcaatcaaaa gtggtatgcc gtcttgatga aaatctcttg
    14461 gaataagctg gaaaagggca gagaaggaca agtggaagca gtcaacctca agcatgacca
    14521 agtagctaat ttgctttcac aaaaggggat ttatccagcc ttccatatga gcaagcgcta
    14581 ctggattagt gtgtcccttg atgatacttt atcagatgaa gaagtactgg aattgataga
    14641 aaaaagttgg aacttaacct ctaaaaaatg aaatatttta ataattttca tgaactttca
    14701 attagctaaa tattctttac tgaagaaatt tttagaaaat ataggattta ccacactaga
    14761 ggaatatggt gccatcttca aatacctgat tgagaatgtc aagacggatc gtcagatcat
    14821 ctgttcgcct cactgtcatg atgacctcgg aatggcagtg gcaaatagcc ttgctgctgt
    14881 caagaatggt gcaggacgtg ttgaagggac tatcaatggt attagggagc gagctgaaaa
    14941 tgctgctttg gaagaaattg cagtggctct caatattcgc caagattact accaagtaga
    15001 aaccagtatt gtcctaaatg agaccatcaa tacgtcagaa atggtttctc gcttctctgg
    15061 tattccagtt cctaaaaaca aagccgtcgt tggtggcaat accttctccc acgaatctgg
    15121 tattcaccaa gatggagtcc ttaaaaatcc tctcacttat gagatcatca cacctgaatt
    15181 ggttggtgtt aagattccgc ttggaaaatt atctggtcgc catgcttttg ttgagaaact
    15241 gagagaattg gccctagatt ttacagaaga ggatatcaaa ccactctttg ctaagttcaa
    15301 ggcactggcc gataagaagt aagaaatcac agatgcagat cctcgagctt tggtagctgg
    15361 aaccatggtt gaaaatccag aaggcttcca ctttgatgat ttacaacttc aaactcatgc
    15421 agataatgac attgaagcgc tcgttagcct agccaatatg gatggtgaga aagtcgaatt
    15481 taatgcgaca gggcaaggtt ccgttgaagc aatctttaac gctatcgata agttctttaa
    15541 ccaatctgtt cgtttggtgt cctacactat caatgcggta acagatggaa tcgatgccca
    15601 ggatcgggtt ttggtcactg ttgaaaacag agatacagaa accatcttta atgcagcagg
    15661 gcttgatttt gatgtgttga aggcttctgc tattgcctat ataaacgcta atacctttgt
    15721 tcaaaaagag aatgcaggtg agatgggacg cagtgtttct taccacgata tgcctagtgt
    15781 gtaaaggaga aggctatggc aaagaaaata gtagctctag taggagacgg aattggccca
    15841 gaaatcatgt aggctggttt agaagttctg gaggctctag ctgaaaaaac aggttttgac
    15901 tatgagattg acagacgacc gttcggaggt gcagatattg atgcagcagg acctccctta
    15961 cctgatgaaa cccttaaggc aagtagggaa gcagatgcta tcctactagc agctatcggt
    16021 agtcctcagt atgatggagc agcggttcgc cctgaacaag gcctgatggc tctccgtaag
    16081 gaactcaatc tttacgctaa tattcgtcct gtaaaaatct ttgacagtct caagtatttg
    16141 tcaccactca aaccggaacg aatttctggt gtagactttg tcgtggtgcg tgaattgaca
    16201 ggcgagattt actttggaga tcatattctt gaagagcgca aagcgcgtga tatcaacgac
    16261 tatagctatg aggaagtgga gcggattatt cgcaaagcct ttgaaattgc aagaaatcgc
    16321 agaaaaatcg ttactagtat cgataagcaa aatgttctag cgacctcaaa actctggcgg
    16381 aaagtagctg aggaagtcgc acaggatttc ccagatgtaa ccttggaaca ccagctggta
    16441 gactcagctg ctatgcttat gattaccaat cctgctaagt ttgatgttat tgtaacggag
    16501 aatctttttg gagatatttt atctgatgaa tcaagcgtct tatctggtac acttgaggtt
    16561 atgccatcag ccagtcattc tgaaaatgga ccaagtctct atgaacctat tcacggttca
    16621 gcacctgata ttgcaggtca aggaattgcc aatcctactt ccatgatttt atcagttgcc
    16681 atgatgttga gagatagttt cggacgttat gaggatgcag agcgtatcaa acatgctgtt
    16741 gagacaagtc tggcggcagg aattttaacg agagatatag gaggtcaggc ttcaacaaag
    16801 gaaatgacgg aagctattat tgcaaggtta tgaagttaga cgaaaaaatt actctagtcc
    16861 ttttgatttg gaatgtcatc attttcttga tttatggcat tgacaaattc aaggcaagaa
    16921 gaagaacttg gcgcatccaa gagaaaatct tactcatttt agcccttact tgtggtggtt
    16981 ttggggcctg gttagctgga atcacttttc accacaagac tcgaaaatgg tactttaaaa
    17041 cagtttggtt tcttgggatg gtgaccacac tagtagcctt atattttatt tggaggtaat
    17101 ggatggcagg gtcttcgagg gaacacgctg cttgggttct agcggactat ggttttaagg
    17161 tcgtgattgc aggatctttc ggtgacattc attacaataa tgaactcaat aatggcctgt
    17221 tgccaatcgt tcagcctaga gaggttagag agaaactagc ccagctaaaa ccaaccgacc
    17281 aggtaactgt ggacttggaa caacaaaaaa tcatctcacc agttgaagaa ttcaccttcg
    17341 agatagatag cgagtggaaa cataaactcc taaatagttt ggatgatatc ggtattacct
    17401 tgcagtatga agagttgatt gctgcttatg aaaaacaacg accagcctac tggcaggatt
    17461 agaaaaaata gaaaaggaaa tatagtaaac tgaaataaga tgtaaacaaa tgaattagag
    17521 cttaacatcc atttccagca attttttaga aactacagtg gactattctg gattcaacac
    17581 attataaaat tatgacaaaa cacattcaca agaaggctac gacattttaa aaggtgaggg
    17641 cggatgtatc gtttgcccta ctaaagttgg ttacattatc atgaccagca acaaggcagg
    17701 acttgagcgt aagttcgcag ccaaagaacg taagcgtaac aaaccaggtg ttgttctctg
    17761 cggtagcatg gatgaacttc gcgctttagc gcaactcaac ccagaaattg aagcattcta
    17821 ctaaaaacat tggatgaaga tattcttctt ggttgtatcc ttccttggaa accagaagcc
    17881 tttgaaaaac tcaaagcata cgggaatggc cgtgaagaac ttatgactga tgtacgtggt
    17941 actagctgtt ttgttatcaa gtttggaaaa gcaggtgaac aattggctgc caagctttgg
    18001 gaagaaggta aaatggtcta cgcctcatat gcttcaatga caaaacgatt gaaactcgct
    18061 atgagagcaa ggtgtaatgg tgtctatggt cgataaggac ggcaaactca tcccagaaca
    18121 aggaggagca cgttcaactt caccagcacc agttgtgatc cgtaaagggc ttgacattga
    18181 taaaatcatg atgcacctgt cagatacttt taactcatgg gactaccgtc aggttgagta
    18241 ttattaagat aaaaaaagaa gtctagtgtt atgagatatt aaagctccta acactggact
    18301 ttttgtttag aatttctttt ctttttctat aggatatggt attctatgta gaaaatatat
    18361 gttaataagt aatgccaata tttaaacatc attagtaaaa ggagttagat tgatgaataa
    18421 aagaaaagtt agtttagaag atttttataa atggtatagt ctaaataaag aagagttatt
    18481 aaataaggca actgttggtg aaaagtttaa tgataaatta aaagaagagt ttctccagga
    18541 atggcctttg gataggattt taacaatgtc aatcgatgaa tatgtaatag gaaagggaca
    18601 gcaaaataag tctttatgct acgctcttga gaagggaaaa tacaaaaatc tatttcttgg
    18661 aatttctggt ggctcagctt caaaatttgg tatttattgg aataaaaaaa caaacaaata
    18721 taaagatcaa gctaataatg agatttcaga gttggatcag cgattttcaa aattaaaatc
    18781 agatttgtat gaaattatca aagaaggtat tcgttttaac tttgaaaatc ctatttttga
    18841 tatgaaaaga tcaacaaatg aatttattgg tcgttctgct atggtgacaa aattactttg
    18901 tatctatact gagggagatc ctttctttgg tgtaaatatt aatagtcaga aagaattttg
    18961 gaaccacttt gtttctcaga caaatcaagg tggaccttat ctgcaaaatc ataaaataat
    19021 tgaactagtg tccaaaactt atcctgagtt ggagccatcg aaattaggaa ctatgctttt
    19081 tgagtattct aagcttttta tggaaaataa ggaagacaat agtacaatgg attcatcaaa
    19141 caattttcgt catcaattaa ctcaatctct attaaagtct ccaaacctca tcctccgtgg
    19201 tgctcctggc acaggaaaga cttatcttgc taaagaaatt gccaaagaat taacggatgg
    19261 caacgaagac caaatcggat ttgtacaatt tcacccatcc tatgattata cggattttgt
    19321 agaaggttta agaccagtat caaatgggga tggagctatt gagtttaggc tacaggacgg
    19381 tatttttaaa gatttttgtc agaaagcaaa agaaacccaa ttgattggag gacaagataa
    19441 ttttgatgag gcttgggatt cttacttaga atatataaat gttgctgaag aaaaagaata
    19501 tataacaaaa acatcttact tatctattaa tagtagacaa aatttgtcag taaattatga
    19561 tagtggtgtt tcaggatggt cactacctag caaatatgtt tacgagttgt ataaagataa
    19621 aaattataat aagcaagaat actacaaaag tggtggaaaa actgtcctag aaacattgag
    19681 aaagagattt ggtttgaaag actatgtttc cccaacagaa attgatactg ataagaaatt
    19741 cgtcttcatc atcgatgaaa tcaatcgtgg ggagatttct aagatttttg gcgaactctt
    19801 tttctctatc gaccctggct atcgtggtga aaaaggaagt gtttctaccc aatatgcaaa
    19861 tctacacgaa actgatgaaa agttctatat ccccgaaaat gtttacatca tcggaactat
    19921 gaatgatatt gatcgttcag tggatacctt tgattttgct atgcgtcgtc gttttcgttt
    19981 tgttgaagtt actgccgagg gtcaagttgg catgttggat aaagagctga atatccatgc
    20041 agaagaagca aaaattcgtc taagaaactt gaacgctgct atcgaaaatg ttcaggaatt
    20101 aaacagtcat tatcatattg gaccaagtta ttttcttaag ttgaaggatg tagattttga
    20161 ctatgaatta ctctggtctg attatattaa gcctctccta gaagactact tgcgaggttc
    20221 ttatgatgag gttgaaactt tggaaacttt gaaaaaagca tttgaactga caaataatga
    20281 gcaaaaagat caggcagtag ctgatgacaa tgaaggtgat gaaaacgatg atgcggatta
    20341 ctgataatca acacaagatt attaaagaaa aatttgttga agaatatcct aaactaagca
    20401 atcttctttt agacagaacc ttggaaagtc tatcccaaga tgaacgtatt ttcatttttc
    20461 caaatgattt gactcatact cctgatttgg ataaggacca aaagattttt gaaacagtca
    20521 atcagaaaat caagacaggg aacgtgattg gttttcttgg atatggtcag gaaagattaa
    20581 cgatttcctc acgattttct gatgagagta atgaccattt tttgcattat ctcttaaaca
    20641 aggttcttta tatcaatctc actagtttag atgttgcttt gtctcgtgaa gagaggcttt
    20701 atcaactttt gatgtatctc ttccccaagt atctacaagc tgctattcga aaaggtcttt
    20761 ataaggaata tcatcgattt tttcataacg acagtaatgt taagggagtg attgatgtaa
    20821 gaagccatct caagaaaaat cttcctttca cgggaaatat tgcctacgca acgagagagt
    20881 tcacctatga taatcccctc atgcagttgg tccgtcacac tattgaatac attaagaatc
    20941 agaaaagcat tggtcaaggg gtactagata atctctcaac tagtcgtgaa aacgtatctg
    21001 aaatcgtgcg tgtaacgccc tcttataaac tagctgatcg tgctaagatt actcggagaa
    21061 atcaatctaa acctatacgt catgcatact ttcacgagta cagaaactta caagaacttt
    21121 gtctgatgat cctaaaccaa gaaaagcacg gtttagggta tcaagatcaa aaaatctatg
    21181 gtattctctt tgatgttgcc tggctttggg aagagtatgt ttacaccttg ttgccaaaag
    21241 gttttgtaca tcccagaaat aaggataaga cggatggaat ttcagtattt tctgttggga
    21301 aacgaaaagt atatccagat ttttatgaca gagaacgaaa gattgttcta gatgcaaaat
    21361 ataaaaaact ggaattgact gaaaaaggaa tcaaccgtga ggacttattc cagctgattt
    21421 cctattctta tattttaaaa gctgagaagg ctggactgat ttttcctagt atggagcagt
    21481 cagtaaatag tgaaatagga aaagtagctg gctatggagc tcaattgaag aagtggtcta
    21541 ttcgaatccc tcagaatgcc tcatcctata gtgcattttg taaaatgatg gaaaattcag
    21601 aagaaaattt taaagcgatt attgatgaag aagtggggag aaaataattt cccctctttc
    21661 ttttattttt aacacaagta aaacccgaac gatatatttt aattcataaa aaaaattgac
    21721 aaaaactgta ctttggttta taataaatta aggaaacgtc ggaaaaggcg tagtgatgcg
    21781 agagcatagg taggtcatta caaaagaaac gagacatcga tatgttaaat gaatttccaa
    21841 tttttgatta cgaagatatt caattgattc caaataaatg tgtcattaaa agccgtgcag
    21901 aagcggatac aagtgtcact ttaggaaatc acacctttaa actacctgtt gtgccagcga
    21961 atatgcagac gattttggat gaaaatgtag cagagcaact ggctaaaggt ggttacttct
    22021 acattatgca ccgttttgat gaggcaggac gcattccttt tattaaacga atgcacaatc
    22081 aagggctcat tgcttccata tctgtcggtg ttaaggacta tgagtatgat ttcgttagac
    22141 agctcaagac tgatgctcct gagtatatca ctattgatat tgctcatggt catgcggata
    22201 gcgtgatttc tatgattcaa cacatcaaga aagaattgcc agataccttt gtcattgctg
    22261 gaaatgtggg aacaccagaa gctgtgcgtg aattggaaaa tgctggtgcg gatgctacta
    22321 aggtcggaat cggtcctggt aaggtttgta tcaccaaggt taagactggt tttggtacag
    22381 gtggttggca gttggctgct ctacgctggt gtgtcaaggc tgcgcgtaaa ccgattatcg
    22441 ctgatggagg aattcgtact cacggtgata ttgccaagtc tatccgcttc ggtgctagca
    22501 tgatcatgat tggttccctc tttgcaggac atattgaaag tccagggaaa acgattgaag
    22561 tcgatggtga acagttcaaa gaatattatg gttcagcctc acaatatcaa aaaggtgctt
    22621 acaaaaatgt ggaaggcaaa cgtatcttgc ttcctgctaa aggtcatctg caagacactt
    22681 taactgagat ggaacaagac cttcaaagtg ctatttctta tgcaggtgga cgtcaggttg
    22741 ctgaccttaa acacgttgat tatgtgatcg tgaaaaactc catctggaat ggggatgctt
    22801 cccactaaga cggggtcttt gactaaaaaa cttgttatta gagcaaattt ctgttataat
    22861 aaaacaagtt tccaccctta gtgtaatgga tatcacgtaa gattccggtt cttgagatgg
    22921 gggttcgatt ccctcagggt ggatgtaaac attctaaaaa gcctttaata gggctttttt
    22981 ctttacactg ctctaaattt accgctaaaa ctgagaatct ctgtctaaag aatttccaaa
    23041 tgctttccag tcccgtttta gagtgactca gggggctttt tttgatataa taaaaaggac
    23101 tgttatcagt tagaaagagg ttggtatgaa agaattacaa actgtactaa agaaccattt
    23161 tgcaatcgaa tttacagaca aaaagttact ggaaactgcc tttactcata cgagttatgc
    23221 caatgagcac cgcctcttaa aaatttcaca taatgaacgc ttggaatttt taggagacgc
    23281 tgttctacag ttattgattt cagaatatct gtataaaaaa tatcctaaaa agcctgaagg
    23341 tgacctatca aaactccgtg ctatgattgt ccgtgaggag agtttagctg gttttgcgcg
    23401 tgattgccag tttgaccagt ttatcaagtt gggtaaaggg gaagaaaaat ctggtggtcg
    23461 caatcgtgac accattcttg gtgatgcctt tgaagccttt cttggtgccc ttcttttgga
    23521 taaggatgtg gccaaggtca aggaatttat ctatcaagtc atgattccta aggttgaagc
    23581 aggcgaattt gagatgatta cagactataa aacccatctc caagagttac ttcaggtcaa
    23641 tggtgatgtg gctattcgtt atcaggtgat ttctgaaaca gggcctgctc acgataaggt
    23701 ctttgatgta gaagttcttg ttgaaggtaa gagtatcggt caaggccaag gtcgttctaa
    23761 gaaattagca gagcaggaag ctgccaaaaa tgccgttgag aaggggctgg attcatgtat
    23821 ttaaaggaaa tcgaaattca ggggttcaag tcttttgctg ataagaccaa ggtcgttttt
    23881 gaccaaggtg tgacggcagt tgttggacca aatggatctg gaaagtccaa tattacagaa
    23941 agtctgcgtt gggctttggg ggagtctagt gtcaagagtc tccgtggggg caagatgccg
    24001 gatgtcatct ttgctggaac agaaagtcgc aaaccgctca attatgcttc tgtagttgtg
    24061 actctggata atcatgacgg atttatcaag gatgcaggtc aagaaatcag ggtggaacgc
    24121 catatctatc gtagtggaga tagcgaatac aagattgacg gcaagaaagt ccgtctgcgt
    24181 gatattcatg acctcttctt ggatactgga ttgggacgag attccttctc tattatttcc
    24241 caagggaagg ttgaggagat ttttaattcc aagcctgagg aacgacgagc tatttttgaa
    24301 gaagctgctg gagttttaaa atacaagact cgcagaaaag aaaccgagag taaactgcag
    24361 caaactcagg ataatctgga ccgcttagag gacattatct acgagttgga taatcaaatc
    24421 aagcctcttg agaagcaagc tgagaatgcc cgtaagtttt tagacttgga aggacaacgt
    24481 aaggctattt atttagacgt tctggttgct caaatcaagg aaaataaggc tgaactagag
    24541 tcgacagaag aagagttggc tcaggttcaa gaactcttga tgagttatta ccaaaagcgt
    24601 gaaaaattag aagaagaaaa tcaaactctt aaaaagcaac gccaagattt acaggctgaa
    24661 atggccaaag accaaggcag tttgatggat ttgaccagtc tgattagtga cttagagaga
    24721 aaattagccc tatcgaaact ggaatctgag caagtagccc tcaatcaaca ggaagcacaa
    24781 gcccgtttgg ctactttgga ggataagaga agttcactca gccaagaaaa atctgataaa
    24841 gaaagctctt tagccctctt ggaagaaaat ctagtccaaa ataatcaaaa actcaatcga
    24901 ttagaggctg aattgctagc tttttcagat gatcctgatc agatgattga gctcttacgt
    24961 gaacgctttg tggccctttt acaagaagaa gcagatgtct caaaccaact gacccgtatt
    25021 gagaatgagt tggaaaatag tcatcagctt tctcaaaaac aagcagatca actagaaaag
    25081 ctgaaagaac agttggctac agctaaagag aaggctggtc agcaaaaggc tgagcttgaa
    25141 actgccaagg agcaggttca gaaattattg gccgactatc aagtctgtgc taaggagcaa
    25201 gaggagcaga aaacttccta tcaagctcaa caaagtcaac tctttgaccg tctggatagt
    25261 ctcaaaaaca agcaggctag agcgcaaagt ttggaaaata tcctgagaaa tcatagtaac
    25321 ttttatgcag gtgttaagag tgttctccaa gagaaagatc gtctaggtgg gattattgga
    25381 gcagttagtg agcacctgac ctttgatatg cattatcaaa ctgccctaga aattgcgctt
    25441 ggagcgagta gtcagcacat tattgtagaa gatgagaatg cggcaaccaa agccattgat
    25501 ttactcaaac gaaacagatc tggtcgtgca accttccttc ctttgaccac tatcaaggct
    25561 cgtacgattt ctagtcagaa ccaagatact attgctgcaa gtccaggatt tctgggcatg
    25621 gcagatgagc tggttacttt cgatactaga ctggaagcca ttttcaagaa tttgctagct
    25681 acaacggcca tttttgatac cgtggagcat gcgcgtgcag cagcgcgcca ggttcgttat
    25741 caggttcgta tggtgacatt ggacggaaca gagctgcgca caggtggttc ttatgcaggt
    25801 ggagctaatc gccagaataa cagtattttc atcaagccag aactggagca attacaaaaa
    25861 gaaattgctg aagaagaagc aagtttgcgt tcagatgaag tgagtttgaa gaacttgcaa
    25921 gatgagctag ctagattgac agaaagatta gaagccatca aatctcaggg agagcaggct
    25981 cgtattcagg agcaaggctt gtccctcgcc tatcagcaga ccagtcagca ggttgaagag
    26041 ctagaaactc tttggaaact ccaagaagag gaattaaatc gtctttctga gggagaatgg
    26101 caagcggata aggaaaaatg ccaagagcgc cttactacaa tcactagtga aaagaataat
    26161 ctggaagctg agattgaaga gattaagtct aataaaaacg ccatccaaga acgttatcaa
    26221 aacttgcagg aagagctagc tcaagctcgt ctacttaaga cagaactgca agggcaaaaa
    26281 cgttatgaag ttgctgatat tgaacgctta ggtaaggaac tagataatct tgatattgag
    26341 caagaagaaa ttcagcgcct tcttcaagaa aaggttgaca atcttgagaa agttgatacg
    26401 gatttgctca gtcaacaggc tgatgaagcc aaaactcaaa aaacaaatct ccaacaaggt
    26461 ttgattcgca agcagtttga gttggatgat attgagggtc aactggatga tattgctagt
    26521 catttggatc aggctcgcca gcagaatgaa gagtggattc gcaagcaaac tcgtgctgaa
    26581 gccaagaaag aaaaggttag cgagcgcttg cgccatctac aaaatcaatt aacagaccag
    26641 taccagatta gctatactga agcactagaa aaagcgcatg agttggaaaa tctcaatctg
    26701 gcagagcaag aggtgcagga tttagagaag gctattcgct cactgggacc tgtcaattta
    26761 gacgctattg aacagtacga agaagttcac aaccgtctgg atttcctaaa tagccaacgt
    26821 gatgatattt tgtctgcgaa aaacctgctt cttgaaacca tcacagagat gaatgatgag
    26881 gtcaaggaac gctttaaatc aacctttgag gccattcgtg agtcctttaa agtgaccttc
    26941 aagcagatgt ttggtggagg tcaggcagac ttgatattga ctgagggcga ccttttaaca
    27001 gctggtgtgg agatttctgt tcaacctcca ggtaagaaaa tccagtcgct taacctcatg
    27061 agtggtggtg aaaaagctct atcggctctt gccttgcttt tctccattat tcgtgtcaag
    27121 accattcctt ttgtcatctt ggatgaggta gaggctgcgc tagacgaagc caatgttaaa
    27181 cgtttcgggg attatctcaa ccgctttgat aaggacagtc aatttattgt cgtaacccac
    27241 cgtaagggga ccatggcagc ggccgattct atctatggag tgaccatgca agaatcgggt
    27301 gtttcaaaga ttgtttcagt taagttaaaa gatttagaaa gtattgaagg atgacaatta
    27361 aactagtagc aacggatatg gacggaacct tcctagatgg gaatggacgc tttgatatgg
    27421 atcgtctcaa gtctctcttg gcttcctaca aggaaaaagg gatttacttt gcggtggctt
    27481 cgggtcgggg atttctgtct ctggaaaaat tatttgctgg tgttcgtgat gacattattt
    27541 tcatcgcgga aaatggcagt ttggtagagt atcaaggtca ggacttgtat gaagcgacta
    27601 tgtctcgtga cttttatctg gcaacttttg aaaagctgaa aacgtcacct tatgtagata
    27661 tcaataaact gctcttgacg ggtaagaagg gttcatatgt tctagatacg gttgatgaga
    27721 cctatttgaa agtgagtcag cattataatg aaaatatcca aaaagtagcg agtttggaag
    27781 atatcacaga tgacattttc aaatttacaa ccaacttcac agaagaaacg ctggaagctg
    27841 gtgaagcttg ggtcaatgat aatgtccctg gtgtcaaggc tatgacaact ggctttgaat
    27901 ctattgatat tgttctggac tatgtcgata agggtgtagc tattgttgaa ttagctaaaa
    27961 aacttggtat cacaatggat caggtcatgg cttttggaga caatcttaat gacttacata
    28021 tgatgcaggt tgtgggacat cctgtagctc ctgaaaatgc acgacctgaa attttagaat
    28081 tagcaaagac tgtgattggt caccataagg aacggtcggt tatagcttat atggagggct
    28141 tataatggca gatataaaat tgattgcatt ggacttggac gggaccttgc tgactactga
    28201 taaaaggctg acggatcgta ccaaggaaac cttgaaagct gcgcgtgatc gtggtatcaa
    28261 ggtcgtattg acaactggtc gtcccttaaa agccatggat ttctttctcc atgagttagg
    28321 gactgacggt caggaagatg agtataccat tacttttaat ggtggattag ttcagaaaaa
    28381 tacaggagaa atccttgata aaacagtctt ttcatatgat gatgtggcac gtttgtatga
    28441 agaaacagag aaattatcac tgcctctcga tgccatctca gaaggaacag tttatcaaat
    28501 ccaatcggac caagaaagtc tttatgccaa attcaatcca gctttgacct ttgttccagt
    28561 ggactttgaa gacttatcta gtcaaatgac ctacaacaaa tgcgtgactg cctttgctca
    28621 agaacccttg gatgcagcca ttcagaagat ttctccagaa ttgtttgacc aatatgaaat
    28681 ctttaaatca cgtgaaatgt tgctagaatg gtcaccaaag aatgttcata aagcaacagg
    28741 tttggcaaaa ctaatcagcc atcttggaat cgaccaaagt caagtgatgg cttgtggtga
    28801 cgaggccaat gacctctcta tgattgaatg ggcaggtctt ggtgttgcta tgcaaaacgc
    28861 tgttcctgaa gtaaaggcag ccgcaaatgt agtgacgccg atgaccaacg atgaggaagc
    28921 tgtcgcctgg gctatcgaag aatatgtgct aaaggagaac taagatatgg gattgtttga
    28981 ccgtctattc ggaaaaaaag aagaacctaa aatcgaagaa gttgtaaaag aagctctgga
    29041 aaatcttgat ttgtctgaag atgttgatcc taccttcaca gaagttgagg aagtttctca
    29101 ggaagaagca gaggttgaaa ttgttgaaca agctgtgttc caagaagagg aaatccaaga
    29161 cacagttgaa gaaagtctgg atttagagcc agttatagaa gtttctcaaa aagaagtcaa
    29221 agaatttcca cactcagaag aagggaatac tgagtttcta gagactgtag aagaaaataa
    29281 ttctgaaatt cttgaagcag aaaggcctca aacagaagaa actgttcagg aaaaatatga
    29341 ccgcagtctt aagaaaactc gcacaggttt cggtgcccgc ttgaatgcct tctttgctaa
    29401 cttccgctct gttgacgaag aatttttcga ggaactggaa gaactgttga ttatgagtga
    29461 tgttggtgtc caagtcgctt ctaacttaac ggaggaacta cgttacgaag ccaagcttga
    29521 aaatgccaag aaacctgatg cacttcgtcg tgtcatcatt gagaaattgg ttgagcttta
    29581 tgaaaaggat ggtagctacg atgaaagcat ccacttccaa gataacttga cagttatgct
    29641 ctttgttggt gtgaatggtg ttgggaaaac aacttctatc ggaaaactag ctcaccgcta
    29701 caaacgagct ggtaagaagg tcatgctggt tgcagcagat accttccgtg cgggtgcagt
    29761 agctcagcta gctgaatggg gccgacgagt agatgttcca gtagtaactg gacctgaaaa
    29821 agctgatcca gccagcgtgg tctttgatgg tatggaacgt gccgtggctg aaggtatcga
    29881 tattctcatg attgatactg ctggtcgtct gcaaaataag gataacctta tggctgagtt
    29941 ggaaaagatt ggtcgtatta tcaaacgtgt tgtgccagaa gcaccacatg aaaccttctt
    30001 ggcacttgat gcatcaacag gtcaaaatgc cctagtacag gccaaagaat tttcgaaaat
    30061 cacaccttta acgggaattg ttttgactaa gattgatgga actgctcgag gaggtgtggt
    30121 tctagccatt cgtgaagaac tcaatattcc tgtaaaattg attggttttg gtgaaaaaat
    30181 cgatgatatt ggagagttta actcagaaaa cttcatgaaa ggtctcttgg aaggtttaat
    30241 ctaatcagaa gcaaaaatcc tgcaaggcat aaacttgcag gaaatttttt tattctaagc
    30301 gaccatcttg acgataggcg atatctggtt gccaagtcca tttggcaccg aatttttcaa
    30361 gtaggtcaaa gctggcttga ggtcccatgc ttccagcttt atagtcatga agtggggcac
    30421 cattttcatc ccagagctct tcaatacggt caatcaactt ccatgatgca ccaacttcat
    30481 cccagtggct aaagttagtt gagttgttat ttaggacatc ataaatcaat ttctcgtatg
    30541 gttctggaga agcaccagtt gcagtcgcat ctgtacggta atcaagtgag ttaggagcca
    30601 agttaaattc ttctcctact tgcttcccat ttaggctaag agagaagcct tctgttggtt
    30661 gaatatagat ggtcaaaata tttggagcaa gtggttctcc aaagatagaa tccatttgtt
    30721 taaagacgat gttgacatga gttccttttt cagtcagtcg tttacctgta cggaaaaaga
    30781 aaggaacact acggaatcga tcgctgtcta caaagaaggc accagatgta aaggtttcag
    30841 ttgttgattc tggattcaca tttggctcgc tacgataaga gatgtatttc atgccatcaa
    30901 tcttaccaga gcggtattgc ccacggataa agtgttcttt gagttcttca tcagttggat
    30961 gatagaggtt tttaaagacc ttaatctttt cagcacgaat ctcgtctttt gtgaagcttg
    31021 ctggtttgtc catggcgagg agcgaaagaa gttgtagagt gtggttttgg accatgtcac
    31081 ggagggcacc ggattggtca tagtagccac cacgttcttc tacacccaag cgctccgcaa
    31141 aggtaatttg aacattgtcg ataaaatcct tgttccaaac gttttcaaaa atcaagtttg
    31201 caaagcgaac tgcaaagatg ctttggatca tttccttacc aagataatgg tcgatacgga
    31261 aaatttgttc ttcgtcaaat gttgctagga gttcgtcatt caacttgctt gcagttgcgt
    31321 aatctgtacc aaatggtttt tcaacgatca agcgctcaaa acctttgcca tcgacaatgt
    31381 tttcagattt gaggtgtttg gcaatggttc caaagaactg aggtgccata gacaagaaga
    31441 agagcttatt gtgttcagct tggtattttt cattaagctc agcttgtaat tgacgcaaag
    31501 caatataatg ttccgaatca ttgacatcat ggctttgata gtagaagtgg ctagcaaatt
    31561 cttgggcttg ctcggtacta tctgccaaat caaggatgga ctcgacaact acagattcaa
    31621 aatattcctt actccaaggt ctacgggcag ttccaataac ggcaaagtgc ttggaaagat
    31681 tgccggattt atatagtcta aaaagggaag ggtagagctt gcgtttagcc aggtctccac
    31741 tcgcaccgaa aattgtaaca ataaccttag atgacatcta gctacctaat ttctattttt
    31801 attcctagtc ttgctaggta tgaggaaacc tcatttcata aacttaattc taacataatt
    31861 ttccgaaaaa tcaaaaattc caatacgaag tagaaaaact gcaaggaaat ccttacagtt
    31921 tgagtttaga cgtttaagac cttatctaag aactctttca gacgagggtg ttgtgggtta
    31981 tcaaagattt ggtcaggtgt tccgtcttca aggaactcgc catctgcagt aaagataacg
    32041 cggttggcaa cctgacgagc aaatcccatc tcatgggtta cgataatcat ggtcatacct
    32101 tgctcagcca attccttcat aacgttaagt acgtctccaa ccatctcagg gtcaagggca
    32161 gaagttggtt catcgaagag catgatgtct ggattcattg ctaggccacg agcgatggcc
    32221 acacgttgtt tttgaccacc tgataggcta tctggattgg cattagcttt atctgctagt
    32281 ccaacctttt caagcaactc cattcccaat tcctcagctt cttccttagt catcaacttg
    32341 tgctcaatag gagcaaaggt gatgttgtcc aatacagaca tatgagggaa gaggttgaag
    32401 tgttggaata ccatgccgat attttcacgg acgtggtcaa cattggttgt tttttcagtt
    32461 aaatcatagc cgttcacagt gatgtgaccg ctagtgactt cttctaaaag attgaggcta
    32521 cggaggaaag ttgacttacc agaacctgaa ggaccgatga tacaaacaac atctccttca
    32581 tagaacttag tcgtaattcc ttttaggact tcattttttc catagtgctt gtgtaaatca
    32641 tttacatcaa tttttaattt tgccattaac gaatcctctt ttctaagcgt ttcgctagtc
    32701 tagtcaaaag tgtgataatt acaagataga agatagcaag gattgcatac atcttgaaac
    32761 tttggtagtt acgagcaatg ataatcttac cagtttggaa gagttcaacc aaaccgatag
    32821 cagatacgat agttgtatct ttaagagcga taacgaattg gttgacaaag tttggcaaca
    32881 tcaatttagt tgcttgtggc aagataatct tacgcatggt ttttccataa gagataccca
    32941 agcttcggct ggcttccatt tggccaactg gaacggcctg aataccacca cgaacgattt
    33001 cagcgatata agcagccgca ttgagtgaga gggcaatggt tccagctaca aagtcgttaa
    33061 ttgggctttg ttggcctgtg atagactcga tgaagtttgg aattccccag aagatgaagg
    33121 ctgcaagaat catcaatgga ataccacgaa taacgtcaac gaaaatctca gagatgacgc
    33181 gaagagattt gtatgggcta acgctaaaca taccgaagat aattccgatg acaatggcaa
    33241 tagcaaatga gataagagct agagcaagag tgataccaag accgctaagg agttgtttgt
    33301 agttgttttg aagcaagccc cagagcgttg tttcgtcaac agtacttgtt gaagcagttg
    33361 aagattcgct agctaggtat ttgtcaagaa tcttttggaa ttcaccgttt gctttaaggt
    33421 ttgcaagtcc gttgttgaac atttcaatca gttctggatt tgctcctttt ttaacggcaa
    33481 aggctgtttc accgattgga gttccagaga ttggagtttt caatttttga ccttggctga
    33541 tagaatattt gagaacaggt tcatcatcca taacggcatc aatggcacca gtgtttaaac
    33601 tgtcatacat tgaagaacca tcagcaaagg ttttgatttt gtaaccgtat ttgctttgat
    33661 tttctgttag gaaggtttga gaagcagttc cgtttttaac accgactgtc tttcctttta
    33721 gatcttcata agaagcaata ttgcttgatt ctttgacacc aagaatggta ttagcagtgt
    33781 agtatgattc tgagaagtca aaagttgcct tacgagcatc tgtgacagac atcccagcaa
    33841 tgataccatc ggcttgacca gcttggacag cactgatagc agcatcaaaa ccagggttgg
    33901 tgatttcaat ttcaaaacct tggtctttag cgattgcctt aatcaattcc atatcaatac
    33961 cagtgtattg gttgcttgaa ttttggaaaa caaaaggtgc aaaagaagaa tcgctggcaa
    34021 tgatatattt agccttaaca ggaatagctt ttaatcctgc gagagtagtt gtagttggca
    34081 ctgctgaaga tgatgaagca gtccatttct tgataatttt atcaagacta ccatcttttt
    34141 tcatttcagc caaggcttgg ttaaattcag taaccaggtg ctcgtattta cttccttttt
    34201 tcacaccgaa agcaaaactt cctacagctt caccatccat ttcaatatgg agatcttgac
    34261 cttggttaat ggcatattcg ataacaggtt tgtcatccat catggcatcg atggcaccag
    34321 cactcaagct gttgttcatt aaatcaccag tgtcaaatgt tttaatagta aagccgtatt
    34381 tatctttgat tgtttcaagg aaacgttgag cggcagttcc gtttttaaca ccaacggttt
    34441 tgccagttaa ttggtcgtac ttgctaattt tgtgtgactt tgtagtagca atgacaactt
    34501 ttgtatcata gtaagtatca gacatggtga agactttttc acgttcttta gtctttgtca
    34561 tccctgccat gatagcgtcg gcttgcccag cttgaaccgc attgactgct gcgtcaaatc
    34621 caggatagga catctgaatg ttccagcctt taatctcagc gactttgtta ataatgtcaa
    34681 catcaattcc tttataagtt tgatctgaat ctttaaactc aaaaggtgca taggcggtat
    34741 cagaaacaat cttaatcgtt tctgctttgg caatacctaa tgagaaaatt gggaataaaa
    34801 ttagcaaaaa tgctagaaat tttttcttca tttttagtct ccttttccga atattctctc
    34861 attatatcag aaaaagtaaa aaaaggcaaa ttgttatatt tttcgtgtca gaaaatataa
    34921 cattgatttt tccttttctt tcttgaattg ctattaaaaa gtgctataat aatagtatat
    34981 atagaagaaa agaggacggg gatatgaaga acaaaagaat atttaaagac ttccaagctt
    35041 caaaaatgag tttaaacatt tacacaagcc ccttgttagc ctttgttttt gtcttcatag
    35101 gagagtttgt ggcttttact ttgtatggta ttggcttgtt agctctcatc ggacttgcta
    35161 gaaattttgg agaggctggt caaaatcttg caagctactt gcagaccttg caacagagct
    35221 tgacggataa aacaagtgac tttcgtttaa ttttaggatt actggccttt ggttttattc
    35281 ttaacactgt gttcagatgg acaagaaaag ttgagaaaag acctattcga accttgggat
    35341 tttatagaga gaatttcctc agcaatcttc tgaaaggatt tagtctaggc ttggcacttt
    35401 ttcttctgac cttgttaggt ttagtggtct taggtcaata tcgtttggaa tccattcact
    35461 tgaatcctta ttctcttgcc tttgtcgtct ttactatccc attttggatt ttacagggga
    35521 cagcagaaga agtggtggcc cgtgcttggc tacttcctca attggcctca agaaccaatc
    35581 taaaactagc tattcttata tctagcctgt tctttaccct gcttcatatg ggcaattcta
    35641 gcctcacccc tctatctcta gtaaatctct ttttattcgg agttgccatg gctctttatc
    35701 ttctcaaaac tgatacagtt tggggtgttg caggtattca tggtgcttgg aattttgctc
    35761 agggtaatct ctttgggatt ttagttagtg gtcaaccgtc aggaacgtct ctgatgacct
    35821 ttttaccaca aggcaatcaa gattggctat caggtggttc ttttggcata gaaggttcca
    35881 ttatgacaag tctggtatta ctactgctga ttgtctatct tgctaataaa ttaaagaaag
    35941 aaaatgaaag gatgtgactt cggtccgtcc ttttcttcgt gaaaatacta taagtatgct
    36001 aaaataggaa tagcacatgg agagaggatt cttatgatca atcacattac agataatcaa
    36061 tttaaactag tatcaaaata tcaaccatca ggagatcaac cccaagctat cgagcagttg
    36121 gtggataaca ttgagggggg agaaaaagct cagattctga tgggggcgac tggaacaggg
    36181 aagacctata ctatgagtca ggtcatttct aaagtcaata aaccaactct ggttattgcc
    36241 cacaataaaa ctctggctgg tcagctctat ggggagttta aggaattttt ccctgaaaat
    36301 gcagttgagt atttcgtatc ctactatgat tattaccagc cagaggccta tgtcccttct
    36361 agcgatacct atattgagaa ggatagttct gtcaatgacg agattgacaa gcttcgccac
    36421 tcagctacct cagccctttt ggagcgtaat gatgttattg tcgtggcctc agtctcttgt
    36481 atctatggtt tgggttcgcc caaggaatac gctgatagtg tcgttagtct ccgtcctggt
    36541 ctagagattt ctcgtgataa actcttgaat gacttggtcg atattcagtt tgaacgtaat
    36601 gatattgatt tccaacgcgg aagatttcgt gttcgtgggg atgtggtaga gattttccca
    36661 gcttcccgag atgaacatgc ctttcgagta gaattttttg gagacgaaat tgaccgtatt
    36721 cgtgaagttg aggctctgac aggtcaggtg ttgggagaag tggatcattt agcgattttc
    36781 ccagcgacac actttgtgac caatgacgac cacatggaag ttgccattgc aaagattcag
    36841 gccgagttgg aggagcagtt agctgtcttt gaaaaggaag gtaaactgct tgaagcccag
    36901 cgtttgaaac agcggacaga gtatgatatc gaaatgttgc gtgagatggg ctataccaat
    36961 ggggttgaaa attattctcg ccacatggat ggacggagcg aaggagagcc tccttatacg
    37021 cttctcgact tcttcccaga tgatttcttg attatgattg acgagagtca tatgaccata
    37081 gggcaaatca agggcatgta caatggagac cgttcgcgta aagaaatgct ggttaattat
    37141 ggtttccgtt tgccgtctgc tttggacaat cgtcctctcc gtcgggagga gtttgagagt
    37201 cacgttcatc agattgttta cgtttcagcg acacctggtg actatgaaaa tgaacagacc
    37261 gagacagtga ttgagcaaat cattcgtcca acgggactct tggatccaga ggtggaagtc
    37321 cgtccgacta tgggacagat tgatgacctc ttgggtgaaa tcaatgcccg cgttgaaaaa
    37381 aatgagcgta cctttatcac aactttgacc aagaaaatgg cagaggactt gaccgactac
    37441 ttcaaggaaa tgggtatcaa ggtcaagtac atgcactcgg atatcaagac cttggaacgg
    37501 acggagatta tccgtgacct gcgcttgggt gtctttgatg tcttggtcgg aattaacctg
    37561 ctccgtgaag gaattgacgt tcctgaagtg agcctcgtag ctattctcga tgctgacaag
    37621 gaaggtttcc ttcgcaacga acgtggactc atccagacta ttggacgtgc tgctcgtaac
    37681 agtgaaggcc atgtcatcat gtatgcagac acggttaccc agtctatgca acgtgctatc
    37741 gatgaaactg cccgccgtcg gaaaattcag atggcctata atgaagagca tggtatcgta
    37801 ccacagacaa tcaagaaaga aatccgtgac ctgattgccg tgaccaaggc agttgctaag
    37861 gaagaggaca aggaagtcga catcaatagc ctcaacaaac aagagcgtaa agaactcgtc
    37921 aagaaactag aaaaacaaat gcaagaagcc gtcgaagtgc ttgactttga actggcggct
    37981 cagatccgtg atatgatgtt ggaagtcaag gccttggatt aggggaatag tatgatttat
    38041 ttaagaaagt taaagagaga agatttgatg tctttatggg aaatggctta ttcacaactt
    38101 aatccagttt ggaaacagta tgatgctccc tattatgatg attatcagta tttttcaaat
    38161 tttaaagaat tcgaactaca aaaatcagaa tccattttaa gcaactcaaa tcgccttggt
    38221 atttttgttg atgataaact agttgggact gtttcgcgtt attgggtatg taaacaaaca
    38281 agatggatgg aattgggaat tggtatttat gataaaaaat tctggaacac tggtattggg
    38341 aaagttgcta tgttgcagtg gatagatagg acgtttcagg attacttgga gttggagcat
    38401 ctgggtttga caacttggtc agggaatctt ggtatgatga aacttgctga aaaattaaga
    38461 atgaaaaaag aagctcatat tccaaaagtt cgttattatc aaggtaaata ttttgatagt
    38521 attaaatatg gtattttgag agaagactgg gagaaaataa atgacggtta ttatcaaatc
    38581 aatggaaact cctaaagaga tagaaggtaa atcccttgtt cactggcaaa tgtggagaga
    38641 ggcttatgat gaccttttgc ctgcagaatt tcaggagaca atgacattag aaagatgtcg
    38701 actctttagt caaaagtatc cagaaaatac attgattgcg ataggtgatc tgaaggtagt
    38761 tggttttatc agttatggta actttcgtga tgagactatg caagctggtg aaattattgc
    38821 tttatatgtt ttaaaagact attatggaaa aggtatcgca caaaagttaa tgaaagcagc
    38881 tttgactgct cttaatcatt tttctgaaat tttcttatgg gtattgaaag ataacaagcg
    38941 cgccattgct ttctatcaaa aaatgggttt tacttttgat ggacaagaaa aaatacttga
    39001 acttggaaag cctataaagg aaaaacgaat ggtgttctat tctaaataat tttaaaaaag
    39061 taaaagataa tatctctggt agtaagtctg aaaatttaat aaattagaaa gcgagtaaat
    39121 ttatgtcccg ttcccaatta acaattttaa caaatatctg tctgattgaa gacctcgaaa
    39181 ctcagcgcgt ggtgatgcag tatcgcgccc ctgaaaacaa tcgctggtct ggttatgcct
    39241 ttcctggagg tcatgtagaa aatgatgagg cttttgcgga gtctgtcatt cgtgaaatct
    39301 acgaagaaac agggttgact atccaaaatc ctcaacttgt cggcattaaa aattggccac
    39361 tagatacagg tgggcgctat attgtcattt gttataaggc gactgagttc tctggtagcc
    39421 ttcggtcttc agatgaagga gaagtttctt gggtgcaaaa agaccagatt ccaaacttgg
    39481 atctggccta tgatatgcta ccattgatgg aaatgatgga agctcccgac aagtcagagt
    39541 ttttctaccc tcgccgtaca gaagacgatt gggaaaagaa aatcttctag tcttttacta
    39601 aataacctag ctgatccaag gcctcctcga tatagtggag gtcttgttgt gtttcggctt
    39661 cgactaggtg gtaatggatg ccatctgtca attcagatag aggtctaaag tcagaatgct
    39721 cgacttgttc tagaaaatgt tgcacatctc tgcgacaaga cagtttaagc aaggtttcaa
    39781 tttctccata aacgggatgg tcaatcaagg tattctggac atgcccacca ttatcgacaa
    39841 tagcaaggag ttcctgaccg atttcttcaa cttcatgttt cactttgaaa agtttgtgaa
    39901 cataaggatt ggtatcaatt tgcttataga tgtagccacg attggtggat aggataggag
    39961 cgccatcggc tctcaaaatt gcaatgtcct gcacaatgac ctgacgtgtg acatgaaaat
    40021 gttccgccaa actttggcca ttaagggctt taggagcttc tttcaagagt tggagcaggg
    40081 cttgtttgcg atcttttgtc atagcttttc cttttaacgg cgttttcgaa gcactttata
    40141 gacagctagt gctaatgtat agtctaccat actatggata attgtgccaa atccaactag
    40201 tacaaataga acatagaaca tattttctac attggtaccc gaagttgcgt aaaaaaggac
    40261 acaggctaat acttcagcaa gggcatgaac aacagccaaa acaaaattga aaacccagga
    40321 agattttggt ttatctaggg tatcggggaa tttttgtagg taaagagctc ctaaagcacc
    40381 aaaagatata tgggaaaaag cccgaaaaac gataaccatg ggatagccag ccatcaaaaa
    40441 tccaaaacta gaggctagga tgacaaaaac tgccatcaag ggcgacaaga acatggctat
    40501 aaaaatagcg atgtggctcc ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg
    40561 cataacaatt ggaatcaaaa tcgcaatagc cgttaaaagg gctgtcattg tcataaattg
    40621 tgtctttttc cgtgtattca caagaatctc ctttttaact gcatatacac tagtatggta
    40681 caataaacca gacaataaag caagaattta cttgggttta tagatcattt tttagttaaa
    40741 agttatagta gatggaaact agaatagtcc acctctactt ctaaaacatt gttagcgatt
    40801 tgactgtcct gatcgatttg tcctgttctt atttcgtttt actatagtaa agatttcatt
    40861 aaaaagaaac tgtatagagc aaaatctcca ccttcaggtt tgaaagcgga gattgttttt
    40921 attttttcaa ggtttgtagt cgtggaacaa tggctagggt tagaattgca gaaatgacca
    40981 attcagcaat tgaatttgtt gagataacgg ttgccagaag aagttggata tttccattat
    41041 aaacatttcc aaataggaag aagattcctc caaggacaaa gatagtattt gtcaaggaac
    41101 caagggctcc agctaaaatc agaccagtct tgtttttcat cagtttatag actaagtaag
    41161 gagttaaacc aatcaaaata cgtgggacga tggcaatgat agctgagtag atgtttccgt
    41221 ttggtacgaa gggagagaag aggtagcttg tcggtagaat cgtaatcgtg ttaaccgtca
    41281 agctaagtaa tcccatcaaa aatccaagtg taaccccaac tcgtggacca taaataatgc
    41341 tggcaataat gacaggaata tgaacaatgg tcggtttgat tggaaatgga aaaaggttaa
    41401 agataagtga gctcagaaag tgtatcacga gcatggtagc aaaaaagata gcaatgggtg
    41461 caatattaga gcgttttttc atcgatagtt tcctttattc tttctaaaat aattgtgagg
    41521 tcagctaaag ctcctcgtcc gtggtctcca caagctagta gggattcctt aggagcaatc
    41581 agctgatagc cgtaggtttc taatgttttc agattattct gagttactgg atggtcatac
    41641 atttttgtat tcatagcagg agctattagt ttgggaatat gacttggtag ggctagagct
    41701 gtactggtta ccatgttgtc cgcaaatccg tgagctagtt ttgcaatagt gttagcagtt
    41761 gcaggtacca cgataaataa atctgctttt tttccaagtt cgatatgatt gacctgatca
    41821 ggatagggtt ccttcatgac atccaagtgg acaggattct gtgagagtac ctgtagtgtc
    41881 aaaggttgga taaactctgt agcagcctga gtcattaaga cagtgacttg atggccttgt
    41941 ttttttagag aactgactaa atctgccgac ttataagagg cgattgaacc cgttacagcc
    42001 aagagaatgt ttgccatagt tttcctttct aagaatgata ggcttgaatt ttttcaagga
    42061 ggagttctgc aatttcttct ttagtctgga ctgtttgaag ctgatttttc tcaacaaata
    42121 tagctcggtg ctgatctgct gaaatttgag tcaggtcatt cgcgatgatt aaatctgctt
    42181 gattcttgat aagacttttt cgtgcaatgt caaccagatg atcttcggta acatcaacca
    42241 gcagtttgaa accaatcaga tgaatagtgg gattccattc cttgactagg gatatgattt
    42301 tgggtgtctt tttaaggaac aaaacctgaa cctcatcagt tgaagaaatc ttggcctgat
    42361 gattctgctt gcttaaaaat tcttttagat tggagctagc ctgaacttcc tcaagccctg
    42421 tcatataaac aggagtgtag tcagaaacag ccattgagtg gatcaagacc tgataatcct
    42481 gaacacgttc ttgcatttct attagaaggt ccttggtatt ggtaatttct cgaatactta
    42541 ggttaggatg aggctctggc ttcagagctc gttttgtcgt aattaaacaa acttcatgcc
    42601 ctgcagaaag caaagtctct gtgataattt tccccaagtg acctgtagaa tggttagtga
    42661 tagagcggac gctatcgata gcttcactgg taccgcccga tgtaactaaa attttcatag
    42721 cactattgta cacaaaaata agcggtaagt aaaatgaaag tgataaattt ttggtaaaaa
    42781 cgaagattta ctatagtttc aaactataaa gccatataaa atttaagaaa ggactctaaa
    42841 agccttaaaa acagagatat ttacgaacgt ttagagagtt ttaggatgtt aaaaatcttg
    42901 ttttgtgata taatgaatta tcatataaga ggttagagga attttgaatg aaaacagata
    42961 ttgaaatcgc acagagtatt gagttgaagc caattgttga tgttgtagag aaacttggta
    43021 tttcttatga cgatttggag ttgtacggaa agtacaaggc taaactcagc tttgataaaa
    43081 ttcgtgcagt tgagagcaat ccagtcggta aattgatctt ggttactgcc atcaacccaa
    43141 cacctgcagg tgaaggaaag tcgacgctta ccattggtct tgcggatgcc ttgaacaaga
    43201 ttggcaagaa aaccatgatt gctatccgcg aaccgtctct tggtccagtc atggggatca
    43261 agggtggtgc tgctggtggt gggtatgcac aagttctgcc aatggaagac atcaacctcc
    43321 actttactgg agatatgcat gctattacaa ctgccaacaa tgccctttct gccttgattg
    43381 acaaccactt gcaccaaggg aatgagctgg aaattgatca acgtcgtatc ctctggaaac
    43441 gcgttgtgga cttgaacgac cgtgcgctcc gccatgtgac tgttggtctt ggtggtcctc
    43501 taaacggtat tccacgtgag gatggttttg atattacagt tgcttcagaa atcatggcaa
    43561 ttctttgctt ggcaacggac atcgaggact tgaaacgtcg tttggcgaat atcgttattg
    43621 gttatcgcta tgaccgtacg cctgtttctg taggtgattt gcaggttgag ggtgccttgg
    43681 ctttgatttt gaaggatgct attaagccaa acttggttca gacaatttac ggtacacctg
    43741 cctttgtaca cggtggtcca tttgccaata tcgctcatgg ctgtaactct gttttggcga
    43801 cgacaacagc ccttcacttg gctgattaca ctgttactga agctggtttt ggtgcggacc
    43861 ttggtgctga gaaattcctt gatatcaaga caccaaactt gccaacatct ccagatgcag
    43921 ttgtcattgt cgcaaccctt cgtgccctta agatgaatgg tggtgtggct aaagacgctc
    43981 tgactgaaga aaatgtagag gcagttcgtg caggttttgc taacttgaaa cgccacgttg
    44041 aaaatatccg taagttcggt attccagcag ttgtagctat taacgaattt gtatctgata
    44101 cagaagctga aattgcagtc ttaaaagaac tctgtgcctc aatctatgta ccagttgaat
    44161 tggctagtgt ctgggctgat ggagcagaag gtggagtagc acttgccgaa acagttgtta
    44221 agacaattgc tgaaaatcca gctaactata aacgtttgta cgataatgcc ctttctgtcc
    44281 aagaaaagat tgaaaaaatt gtcactgaaa tctatcgtgg tagcaaagtg aactttgaga
    44341 agaaagctca aacacaaatt gctcaaatcg ttcaaaacgg ttgggacaaa ttgccaatct
    44401 gtatggctaa aactcaatac agtttctcag acaatccaaa tgcgcttgga gcaccagaaa
    44461 actttgaaat taccattcgt gaattggtac caaaattagg tgcaggcttc atcgttgcct
    44521 taactggtga tgttatgacc atgccaggtc ttccaaaacg tccagcagct ctcaacatgg
    44581 atgttgaaag cgatggaact gtactaggct tgttctagtg gattgaaatt gactttaagt
    44641 gagtttggaa ataatatcca agctcatttt ttctcagact gcaagcagtt catcaataag
    44701 atttatactc ttcgaaaatc tcttcaaac
//
LOCUS       DAVKFH010000011        44247 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKFH010000011 DAVKFH010000000
VERSION     DAVKFH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.011"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            99..575
                     /locus_tag="VW006_000643"
     CDS             99..575
                     /locus_tag="VW006_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9379540.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            613..918
                     /locus_tag="VW006_000644"
     CDS             613..918
                     /locus_tag="VW006_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9379541.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            966..2441
                     /locus_tag="VW006_000645"
     CDS             966..2441
                     /locus_tag="VW006_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9379542.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2791..9492
                     /locus_tag="VW006_000646"
     CDS             2791..9492
                     /locus_tag="VW006_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9379543.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9740..10596)
                     /locus_tag="VW006_000647"
                     /pseudo
     CDS             complement(9740..10596)
                     /locus_tag="VW006_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10807..11166)
                     /locus_tag="VW006_000648"
     CDS             complement(10807..11166)
                     /locus_tag="VW006_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9379544.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            11258..12214
                     /locus_tag="VW006_000649"
                     /pseudo
     CDS             11258..12214
                     /locus_tag="VW006_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12198..12755
                     /locus_tag="VW006_000650"
     CDS             12198..12755
                     /locus_tag="VW006_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9379545.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12748..13002
                     /locus_tag="VW006_000651"
     CDS             12748..13002
                     /locus_tag="VW006_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379546.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            13014..15068
                     /locus_tag="VW006_000652"
     CDS             13014..15068
                     /locus_tag="VW006_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9379547.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15247..16107
                     /locus_tag="VW006_000653"
     CDS             15247..16107
                     /locus_tag="VW006_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9379548.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            16318..17028
                     /gene="ccdA2"
                     /locus_tag="VW006_000654"
     CDS             16318..17028
                     /gene="ccdA2"
                     /locus_tag="VW006_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9379549.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            17048..17614
                     /locus_tag="VW006_000655"
     CDS             17048..17614
                     /locus_tag="VW006_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9379550.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17625..18737
                     /gene="msrB"
                     /locus_tag="VW006_000656"
     CDS             17625..18737
                     /gene="msrB"
                     /locus_tag="VW006_000656"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9379551.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            19005..19742
                     /locus_tag="VW006_000657"
     CDS             19005..19742
                     /locus_tag="VW006_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9379552.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19739..21430
                     /locus_tag="VW006_000658"
     CDS             19739..21430
                     /locus_tag="VW006_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9379553.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            21546..22109
                     /locus_tag="VW006_000659"
     CDS             21546..22109
                     /locus_tag="VW006_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9379554.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            22131..27800
                     /locus_tag="VW006_000660"
     CDS             22131..27800
                     /locus_tag="VW006_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9379555.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEALKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            27861..29582
                     /gene="pabB"
                     /locus_tag="VW006_000661"
     CDS             27861..29582
                     /gene="pabB"
                     /locus_tag="VW006_000661"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9379556.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            29702..31228
                     /locus_tag="VW006_000662"
     CDS             29702..31228
                     /locus_tag="VW006_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9379557.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            31315..32234
                     /locus_tag="VW006_000663"
                     /pseudo
     CDS             31315..32234
                     /locus_tag="VW006_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            32369..33328
                     /locus_tag="VW006_000664"
     CDS             32369..33328
                     /locus_tag="VW006_000664"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9379558.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            33425..34264
                     /locus_tag="VW006_000665"
     CDS             33425..34264
                     /locus_tag="VW006_000665"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9379559.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(34312..34482)
                     /locus_tag="VW006_000666"
     CDS             complement(34312..34482)
                     /locus_tag="VW006_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9379560.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            34603..35487
                     /gene="miaA"
                     /locus_tag="VW006_000667"
     CDS             34603..35487
                     /gene="miaA"
                     /locus_tag="VW006_000667"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9379561.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            35480..36718
                     /gene="hflX"
                     /locus_tag="VW006_000668"
     CDS             35480..36718
                     /gene="hflX"
                     /locus_tag="VW006_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9379562.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            36711..37334
                     /locus_tag="VW006_000669"
     CDS             36711..37334
                     /locus_tag="VW006_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9379563.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            37349..38278
                     /locus_tag="VW006_000670"
     CDS             37349..38278
                     /locus_tag="VW006_000670"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9379564.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            38299..39054
                     /locus_tag="VW006_000671"
     CDS             38299..39054
                     /locus_tag="VW006_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9379565.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(39108..40016)
                     /locus_tag="VW006_000672"
     CDS             complement(39108..40016)
                     /locus_tag="VW006_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9379566.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            40080..40349
                     /locus_tag="VW006_000673"
     CDS             40080..40349
                     /locus_tag="VW006_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9379567.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            40349..40729
                     /locus_tag="VW006_000674"
     CDS             40349..40729
                     /locus_tag="VW006_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9379568.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            40818..41045
                     /locus_tag="VW006_000675"
                     /pseudo
     CDS             40818..41045
                     /locus_tag="VW006_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(41085..41810)
                     /locus_tag="VW006_000676"
     CDS             complement(41085..41810)
                     /locus_tag="VW006_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9379569.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            41972..43813
                     /gene="typA"
                     /locus_tag="VW006_000677"
     CDS             41972..43813
                     /gene="typA"
                     /locus_tag="VW006_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9379570.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            43833..44087
                     /locus_tag="VW006_000678"
     CDS             43833..44087
                     /locus_tag="VW006_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9379571.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
BASE COUNT        13698 a         8080 c         9762 g        12707 t
ORIGIN      
        1 ataaaaaaag agacaaaaaa gaacaaaaag taattgacaa cggtttcata tagtgttata
       61 cttatggcat aaagttaatg gaaagaaggg aaaaacttat ggtattagat tatttctttg
      121 acaaaaacct tgtgttttgc ttagaagcgg ataatcaaga acatctcttt gatcaggttg
      181 caactttatt ggaagaacga gaaattgtaa ctccaactta tcgtgaagcc ttgatcacgc
      241 gtgaaaagtc atttccaact ggtttagata tggaatttct aggaaaggac ttgccaaatg
      301 tagcgattcc tcacacagat attgttcata atctagctga gaaagtggtg gttgttcgat
      361 tagaaaaacc agtaactttt cacaatatga tagctcctga taaggaagta gaagtatctc
      421 tactcttctt tatcattaac aattcaagtt caagtcaaac gaacattctg gctcagttga
      481 tggacttttt cacaggaaat ggtcatcttg aagacctatc aaaaatttct gaaccagaaa
      541 aactttatgc ttacattgct gaagcaaccg cttaatcttg tctattaaaa aataaaatcg
      601 gaggaaatcc aaatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac
      661 caaattaaaa gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat
      721 gcagtcatgg tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca
      781 atcgctgcaa cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc
      841 ttattccgta tcccagcaat gagcgctcca gtatttgaca atattgaagc agctattaaa
      901 gaacatggat taagctaatt ctaatttgtt aacattcata taacataaaa aagggaggaa
      961 ctgttatgga tgcaatcttt gacctaatcg gaaaggtttt caatcccatc ttagaaatgg
     1021 gtggacctgt catcatgttg atcattttga cagtattggc tttacttttt ggagtgaaat
     1081 tctccaaagc gcttgaaggt ggtatcaaac ttgccatcgc tcttacaggt atcggtgcta
     1141 tcatcggtat gctaaatggt gctttctcag catcacttgc aaaattcgtt gaaaacactg
     1201 gtatccaatt gagtattaca gacgttggtt gggcaccact cgcaacaatc acttggggtt
     1261 ctgcttggac actatacttc ttgctcatca tgttgattgt caacgtagtg atgcttgcta
     1321 tgaagaaaac cgatacactt gatgtcgata tctttgatat ctggcacttg tctatcacag
     1381 gtctcttgat taaatggtat gctgataaca atggtgtgag tcaaggggtt tcactcttta
     1441 ttgctacagc agctatcgtc cttgtcggtg tgttgaaaat tatcaactct gacttgatga
     1501 aacctacatt tgatgacctt cttaacgccc caagttcatc accaatgaca tcaactcaca
     1561 tgaactacat gatgaaccca gttatcatgg ttttggataa gatttttgaa aaattcttcc
     1621 caggccttga taaatatgac tttgatgctg ctaaattgaa caagaaaatc ggtttctggg
     1681 gatctaaatt cttcatcggt ttcatccttg gtatcgttat cggtattatg ggaactccac
     1741 atccaattgc aggtgttgca gatgcagata aatggcgtct tgttatcaaa ggatggttgt
     1801 ctcttggttt gactgccggt gtatctttgg aactcttctc acttatcggt tcatggttca
     1861 tcgcagccgt agaaccacta tcacaaggta ttacaaacgt tgctactaaa cgtcttcgag
     1921 gacgtaaatt caatatcggt cttgactggc cattcatcgc tggtcgtgct gaaatctggg
     1981 cttgtgccaa cgtacttgca ccaatcatgt tgattgaagc agtgcttctt tcaaaagttg
     2041 gaaatggtat cttgccactt gcaggtatca tcgctatggg tgttactcca gctctcttgg
     2101 ttgtaactcg tggtaaattg ctccgtatga ttatcttcgg aacactcttg ttgccactct
     2161 tccttctttc aggtacactt attgcaccat ttgcaacaga acttgctaaa ggtgtaggtg
     2221 ccttcccaga aggtgtgagc caaactcaat tgattactca ctctactctt gaaggaccaa
     2281 tcgaaaaact tcttggttgg acaattggta acactacaac tggtgatatc aaagcaatcc
     2341 ttggtgcagt agtcttcctt gtattctata tcggtatctt tgcttggtac agaaaacaaa
     2401 tgatcaaacg taacgaagag tacgcagcaa aagcaaaata atgcgctcct ataaaatata
     2461 aactcaatga aaatcaaaga gcaaactagg aaactagccg caggttgcta aaaacacagt
     2521 tttgaggttg tagataagac tgacgaagtc agctcaaaac atggttttga ggttgtagat
     2581 gaaactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc
     2641 agtaatcata tctacggcaa ggcgacgctg acgaagtttg aattggattt tcgcagagtg
     2701 taaattgttg aaactaggtt gtcataccat gtataccact tgggctttgg tagtagctaa
     2761 ctgcactaaa tataatataa ggagggtttt atggggaaag gccattggaa tcggaaaaga
     2821 gtttatagca ttcgtaagtt tgctgtggga gcttgctcag taatgattgg gacttgtgca
     2881 gttctattag gaggaaatat agctggagaa tctgtagttt atgcggatga aacacttatt
     2941 actcatactg ctgagaaacc taaagaggaa aaaatgatag tagaagaaaa ggctgataaa
     3001 gctttggaaa ctaaaaatgt agttgaaagg acagaacaaa gtgaacctag ttcaactgag
     3061 gctattgcat ctgagaagaa agaagatgaa gccgtaactc caaaagagga aaaagtgtct
     3121 gctaaaccgg aagaaaaagc tccaaggata gaatcacaag cttcaagtca agaaaaaccg
     3181 ctcaaggaag atgctaaagc tgtaacaaat gaagaaatga atcaaatgat tgaagacagg
     3241 aaagtggatt ttaatcaaaa ttggcacttt aaactcaatg caaattctaa ggaagccatt
     3301 aaacctgatg cagacgtatc tacgtggaaa aaattagatt taccgtatga ctggagtatc
     3361 tttaacaatt tcgatcatga atctcctgca caaaatgaag gtggacaact caacggtggg
     3421 gaagcttggt atcgcaagac tttcaaacta gatgaaaaag acctcaagaa aaatgttcgc
     3481 cttacttttg atggcgtcta catggattct caagtttatg tcaatggtca gttagtgggg
     3541 cattatccaa atggttataa ccagttctca tatgatatca ccaaatacct tcacaaagat
     3601 ggtcgtgaga atgtgattgc tgtccatgca gtcaacaaac agccaagtag ccgttggtat
     3661 tcaggaagtg gtatctatcg tgatgtgact ttacaagtga cagataaggt gcatgttgag
     3721 aaaaatggga caactatttt aacaccaaaa cttgaagaac aacaacatgg caaggttgaa
     3781 actcatgtga ccagcaaaat cgtcaatacg gacgacaaag accatgaact tgtagccgaa
     3841 tatcaaatcg ttgaacgagg tggtcatgct gtaacaggct tagttcgtac agcgagtcgt
     3901 accttaaaag cacatgaatc aacaagccta gatgcgattt tagaagttga aagaccaaaa
     3961 ctctggaccg ttttaaatga caaacctgcc ttgtacgaat tgattacgcg tgtttaccgt
     4021 gacggtcaat tggttgatgc taagaaggat ttgtttggtt accgttacta tcactggact
     4081 ccaaatgaag gtttctcttt gaatggtgaa cgtattaaat tccatggagt atccttgcac
     4141 cacgaccatg gggcgcttgg agcagaagaa aactataaag cagaatatcg ccgtctcaaa
     4201 caaatgaagg agatgggagt taactctatc cgtacaaccc acaaccctgc tagtgagcaa
     4261 accttgcaaa tcgcagcaga actaggttta ctcgttcagg aagaggcctt tgatacgtgg
     4321 tatggtggca agaaacctta tgactatgga cgtttctttg aaaaagatgc cactcaccca
     4381 gaagctcgaa aaggtgaaaa atggtctgat tttgacctac gtaccatggt cgaaagaggc
     4441 aaaaacaacc ctgctatctt catgtggtca attggtaatg aaataggtga agctaatggt
     4501 gatgcccact ctttagcaac tgttaaacgt ttggtcaagg ttatcaagga tgttgataag
     4561 actcgctatg ttaccatggg agcagataaa ttccgtttcg gcaatggtag cggagggcat
     4621 gagaaaattg ctgatgaact cgatgctgtt ggatttaact attctgaaga taattacaaa
     4681 gcccttagag ctaagcatcc aaaatggttg atttacggtt cagaaacatc atcagcaacc
     4741 cgtacacgag gaagttacta tcgccctgaa cgtgaattga aacatagcaa tggacctgag
     4801 cgtaattatg aacagtcaga ttatggaaat gatcgtgtgg gttgggggaa aacagcaacc
     4861 gcttcatgga cttttgaccg tgacaacgct ggctatgctg gacagtttat ctggacaggt
     4921 acggactata ttggtgaacc tacaccatgg cacaaccaaa atcaaactcc tgttaagagc
     4981 tcttactttg gtatcgtaga tacagccggc attccaaaac atgacttcta tctctaccaa
     5041 agccaatggg tttctgttaa gaagaaaccg atggtacacc ttcttcctca ctggaactgg
     5101 gaaaacaaag aattagcatc caaagtagct gactcagaag gtaagattcc agttcgtgct
     5161 tattcgaatg cttctagtgt agaattgttc ttgaatggaa aatctcttgg tcttaagact
     5221 ttcaataaaa aacaaaccag cgatgggcgg acttaccaag aaggtgcaaa tgctaatgaa
     5281 ctttatcttg aatggaaagt tgcctatcaa ccaggtacct tggaagcaat tgctcgtgat
     5341 gaatctggca aggaaattgc tcgagataag attacgactg ctggtaagcc agcggcagtt
     5401 cgtcttatta aggaagacca tgcgattgca gcagatggaa aagacttgac ttacatctac
     5461 tatgaaattg ttgacagcca ggggaatgtg gttccaactg ctaataatct ggttcgcttc
     5521 caattgcatg gccaaggtca actggtcggt gtagataacg gagaacaagc cagccgtgaa
     5581 cgctataagg cgcaagcaga tggttcttgg attcgtaaag catttaatgg taaaggtgtt
     5641 gccattgtca aatcaactga acaagcaggg aaattcaccc ttactgctca ctctgatctc
     5701 ttgaaatcta gtcaagtcac tgtctttact ggtaagaaag aaggacaaga aaagactgtt
     5761 ttggggacag aggtgccaaa agtacagacc attattggag aggcacctga aatgcctacc
     5821 actgttccgt ttctatacag tgatggtagt cgtgcagaac gtcctgtaac ctggtcttca
     5881 gtagatgtga gcaagcctgg tattgtaacg gtgaaaggta tggctgacgg acgagaagta
     5941 gaagctcgtg tagaagtgat tgctcttaaa tcagagctac cagttgtgaa acgtattgct
     6001 ccaaatactg acttgaattc tgtagacaaa tctgtttcct atgttttgac tgatggaagt
     6061 gtacaagagt acgaagtaga tagctgggag attacggaag tagataaagc caaactttca
     6121 gtagctggat cacgtattca aatgactggt cagttagctg gagaaactat tcatgcaacc
     6181 cttgtggtag aagaaggaaa tgctgcagca cctgtagtgc caactgttac tgttggaggt
     6241 gaagcagtaa caggtcttac tagtcgacaa ccaatgcaat atcgtactct atcttatggt
     6301 gcccaattgc cagaagtcac agcaagtgct gaaaatgctg atgtgacagt tcttcaagca
     6361 agcgcagcaa acggcatgcg tgcaagcata tttgttcagc ctaaagatgg tggccctctt
     6421 caaacctatg caattcaatt cctagaagaa gcaccaaaaa ttgctcactt gagcttgcaa
     6481 gtggaaaaag ctgacagtct caaagaagac caaactgtca aattgtcggt tcgagctcac
     6541 tatcaagatg gaacgcaagc tgtattacca gctgataaag taaccttctc tacaagtggt
     6601 gaaggggaag tcgcaattcg taaaggaatg cttgagttgc ataagccagg agcagtcact
     6661 ctcaaagcgg aatatgaggg agctaaaggc caagttgaac tcactatcca agccaatact
     6721 gagaagaaga ttgcgcaatc tatccgtcct gtaaatgtag tcacagattt gcatcaaaaa
     6781 cctactcttc caacaacagt aacggttgag tatgacaaag gtttccctaa agctcacaaa
     6841 gtcacttggc aagctattcc gaaagaaaaa ctagactcct atcaaacatt tgaagtacta
     6901 ggtaaagttg aaggaattga ccttgaagcg cgtgcaaaag tctctgtaga aggtatcgtt
     6961 tcagttgaag aagtcagtgt gacaactcca atcgcagaag caccacaatt accagaaagt
     7021 gttcggacat atgattcaaa tggtcacgtt tcatcagcta aggttgcatg ggatgcgatt
     7081 cgtccagagc aatacgctaa ggaaggtgtc tttacagtta atggtcgctt agaaggtact
     7141 caattaacaa ctaaacttca tgttcgcgta tctgctcaaa ctgagcaagg tgcaaacatt
     7201 tctgaccaat ggaccggttc agaattgcca cttgcctttg cttcagactc aaatccaagc
     7261 gacccagttt caaatgttaa tgacaagctc atttcctaca ataaccaacc agccaatcgt
     7321 tggacaaact ggaatcgtac taatccagaa gcttcagtcg gtgtcctatt cggagattca
     7381 ggtattttga gcaaacgttc agttgataac ttaagcgttg gcttccatga agaccatgga
     7441 gttggtgcac cgaagtctta tgtgattgag tattatgttg gtaagactgt cccaacagct
     7501 cctaaaaacc ctagttttgt tggtaatgag gaccatgtct ttaatgattc tgccaactgg
     7561 aaaccagtta ctaatctaaa agcccctgct caactcaagg ctggagaaat gaaccacttt
     7621 agctttgata aagttgaaac ctatgctgtt cgtattcgca tggtgagagc agacaacaaa
     7681 ctaggaacgt ctatcacaga ggtacaaatc tttgcgaaac aagttgcggc agccaagcaa
     7741 ggacaaacaa gaatccaagt tgacggtaaa gacttagcaa acttcaaccc tgatttgaca
     7801 gactactacc ttgagtctgt agatggaaaa gttacggcag tcacagcaag tgttagcaac
     7861 aatggcctcg ctaccgtcgt tccaagcgtt cgtgaaggtg agccggttcg tgtcatcgcg
     7921 aaagctgaaa atggcgacat cttaggagaa taccgtctgc acttcactaa ggataagaac
     7981 ttactttctc ataaaccagt tgctgcggtt aaacaagctc gcttgctaca agtaggtcaa
     8041 gcacttgaat tgccgactaa ggttccagtt tacttcacag gtaaagacgg ctacgaaaca
     8101 aaaaacctga cagttgaatg ggaagaagtt ccagcggaaa atctgataaa agcaggtcaa
     8161 tttaccgttc gaggccatgt ccttggtagt gatcttgttg ctgaggtcac tgtacgagtg
     8221 acagacaaac taggcgaagc tctttcagat aaccctaact atgatgaaaa cagtaaccag
     8281 gcctttgctt cagcaaccaa tgatattgac aaaaactctc atgaccgcgt tgactatctc
     8341 aatgacggag atcattcaga aaatcgtcgt tggacaaact ggtctccaac accatcttct
     8401 aatccagaag tatcagcggg tgtgatcttc cgtgaaaatg gtaagattgt agaacggact
     8461 gttgctcaag ccaaacttca cttctttgca gatagtggta cggatgcacc aactaaactc
     8521 gttttagaac gctatgtcgg tccagagttt gaagtgccaa cctactattc aaactaccaa
     8581 gcctacgacg cagaccatcc attcaacaat ccagaaaatt gggaagctgt gccttatcgt
     8641 gcggataaag acatcgcagc tggtgatgaa atcaacgtaa catttaaagc tgtcaaagcc
     8701 aaagctatga gatggcgtat ggagcgtaaa gcagataaga gcggtgttgc gatgattgag
     8761 atgaccttcc ttgcaccaag tgaattgcct caagaaagca cgcaatcgaa gattcttgta
     8821 gatggaaaag aacttgctga tttcgctgaa aatcgtcaag actatcaaat tacctataaa
     8881 ggtcaacggc caaaagtctc agttgaagaa aacaatcaag tagcttcaac tgtggtagat
     8941 agtggagaag atagccttcc agtacttgtt cgcctcgttt cagaaagtgg aaaacaagtc
     9001 aaggaatacc gtatccagtt gactaaggaa aaaccagttt ctgagaagac agttgctgct
     9061 gtacaagaag atcttccaaa actcgaattt gttgaaaaag atttggccta caagacagtt
     9121 gagaaaaaag attcaacact gtatctaggt gaaactcgtg tagaacaaga aggaaaaact
     9181 ggtaaagaac gtatctttac agcgattaat cctgatggaa gtaaggaaga aaaactccgt
     9241 gaagtggtag aagctccgac agaccgcatc gtcttggttg gaaccaaacc agtagctcaa
     9301 gaagctaaaa aaccacaagt gtcagaaaaa gcagatacaa aaccaattga ttcaagtgaa
     9361 gctagtcaaa ctaataaagc ccagttacca aatacaggta gtgcggcaag ccaagcagca
     9421 gtagcagcag gtttagctct tctaggtttg agtgcaggat tagtagttac taaaggtaaa
     9481 aaagaagact agaaattttg ataccttctt tatcattaaa gaccaaagaa aaactcttgt
     9541 cctaaataga agataaagag tctggaacaa aatagttctc aaccacaaaa aagctagata
     9601 tttccaattg cggaactcta gctttttaat tttgagtcgt tctcttattg tatagtagat
     9661 tgaaactaga gtagtacact tctacttcta aaacattgtt agaaatcgat ttgactgtcc
     9721 ttatcgattt gtcctgttct tatttcattt cactatgttt taggaggtta ttggccataa
     9781 gtaccaatcc catgtcaatt ctcacttgac gcttgcctct cagattacat ctcttgtaac
     9841 ccaaacaagc ctttatctgc ccaaagacag gttctacatc aatcttgcgt tgagcgaaaa
     9901 tctgtctatc ttggggagat aaaagcgcct gacattcttt agctttcaag ttttgatagc
     9961 gttcgttcat atacaatccc ttttgagggg ctgattcagg ttcatcagcg tagtaaacct
    10021 tgatttcctg ttgaaagtct gtctgtgttt tctggtactt gatatggtga aaacgatagc
    10081 accagccatc aggatgtatg tagctatcct ccttgtcatt atagtgccaa ttcgctaagt
    10141 ttttagctga ctgtttatac cctctctttt gttccttatc aaacatggca tatttaatca
    10201 gaaggggagt gatttcttct tgaaaataga cttgtatctg ttcttgaagt ttggcggaga
    10261 atttctctgt cgttttcttc cacacaaaac tatacttgtt ggcattggat tcaatcttag
    10321 ttccgtcaat aaacaaacaa tctaaggtca ctaactcttc catttttcaa tttttcgacc
    10381 agagaaaatc ccttgtgaat aggcaaatag aagagtagat acaagcattt tagggtgata
    10441 agacgggcga ccaaaggcat gataggaggt gtagaagtga cgttcctcca aggtattcac
    10501 cactttttca atagtaaaga cgagatggtc ttgtggtaag aaggaactga tttctagtgg
    10561 taaagttgtt tgatttgtgt tatagtgaat atgcatggga tagcctttct aaatggttta
    10621 ttaagcaatt ctattttacc aagactatcg taaccatgca aaaaagcaac ggcaaatccg
    10681 ttgccttttt tggatataag agactattgt cccagactct tttctttgta taaaattgta
    10741 tagtgttatt gtctatacta ttttgtagag aaagcaaaaa tccctagaaa atgcttaggg
    10801 attcgactat ttcattaaga acaattcaac aatcatctct atatacatga taagggttaa
    10861 taaaaatcct gtacgccaga agaatttgat gaatttagga tagtaaaagc tgcgtttttt
    10921 taaaaggaag aaaaagacga gaataatggc taggaacgag agggctagcc ccagtctagg
    10981 gaggaaatta tgggtaaagg ttttagctgt aatcaagtaa tactcaaata ccaagactgg
    11041 aaaagccaaa tccgcaaagt ttcgtcctag tttttttaat ctaaaaagtt tggttaggat
    11101 aatgcagacg actaaggtca gtatcaataa taaaatagat gctaatttca ttaaaatcat
    11161 acccatattg tatcataaaa aaaggctaat ggaaacgaga aacagaggaa tttataggaa
    11221 agtcaggctt ttgagattgt gggtgatttt tgttataatg aaagttataa aatcttatga
    11281 taccttgaat gattattatc gaaaactctt tggagaaaag acttttaaag tccctattga
    11341 tgcgggattt gactgtccca atcgtgatgg aactgtggct catggaggct gtactttttg
    11401 tacggtttcc ggttctggag atgccattgt agccccagat ccgcctatcc gtgagcaatt
    11461 ttataaggaa attgacttta ttcatcgtaa gtggccagat gttcagaagt atctggttta
    11521 ttttcaaaat tttaccaaca cccatgaaaa ggtggaagtt attcgagagc gttatgaaca
    11581 ggctatcaat gagccaggtg tggtaggaat caatattgga acgcgcccag actgtttacc
    11641 agacgaaacc atcgaatatt tggctgagtt atcggagtgc atgcatgtga cggttgaatt
    11701 gggcttgcag actacttatg aagcaacctc tgacctgatt aaccgtgcgc attcttatga
    11761 attgtaggtg gaaacggtca agcgtttgag aaagtatcca aaaattgaga ttgtttccca
    11821 tttgatcaat ggtttgcctg gtgaaaccca tgagatgatg gttgaaaatg tccgccgttg
    11881 tgtcacggat aacgatattc aagggattaa actgcatttg ctccatctta tgacaaatac
    11941 tcgtatgcaa cgagattacc atgagggacg tttacaattg atgagtcagg acgaatatgt
    12001 cagggtcatc tgtgaccaac tggaaattat tcccaaacat atcgtcatcc atcgaattac
    12061 aggagatgcg cctagggata tgctgattgg tcctatgtgg agcctcaata aatgggaagt
    12121 cctcaacagc attgagatgg agatgagacg tcgtggaagt gttcaaggat gcaaggctgt
    12181 aaaacaggag tttgaaaatg aaaagaccac ttgagatggc acatgatttt ttggctgagg
    12241 tcgtgacaaa agaggatgtc gtagtggatg cgactatggg aaatggtcat gacacgcttt
    12301 ttttagccaa gctagccaag caagtctatg cctttgatat tcagaagcaa gccttggaaa
    12361 agacccaaga gcgtttgcat caggctggct tgacaaatgc ccagttaatc ttgcaaggcc
    12421 atgagacact ggaccagttt gtgacaaaag ctaaggcagg gatttttaat ttgggctatt
    12481 tgccgtcagc tgataagtct gtcatcaccc gaccgcagac aacgattgag gcattagaaa
    12541 agctatgtgg cttacttgtc aaaggtggac gaattgctat tatgatttac tatggtcatg
    12601 aaggaggcga cctcgagaga gatgctgtct tggattttgt gatccagttg aaccaacaag
    12661 agtacacagc tgccatttac cgaactttaa accaagtcaa caacccgccg tttttagtga
    12721 tgattgaaaa attagagaga tacagacatg gataaacaat acctacacga aaagctggat
    12781 gccatgcgcc agaattttgt tgaatcaacc taccacgaac gagcggtggg cgtgctagac
    12841 caagcgcata tgagcaaaaa aatgcttaaa atcaagaaaa aattagttgc tcttgaaatg
    12901 gaacggtgcc agagaaaaat tgagcacaaa gactgttcca agattgacca aaaaatcaaa
    12961 gagcagaagg agatatttga atcctgttgt aaaaaagatt aaggaggacg tgcgtggaat
    13021 tactgattta cctcatccta tttttactgg tcttgattgt ctcaagtaca accaataagc
    13081 tcctaccctt tttgcctctc cctttggtgc agattctttt gggaattgtg attggtctct
    13141 ttttacccaa tactgacttt caccttaata cggagttgtt tttggcactg gttatcggcc
    13201 ccttgctttt ccgagaggct gaagaagcag atgttacggc tattttaaaa cactggcgaa
    13261 tcattgttta tctcatattt ccagtgattt ttatctcgac cctgagtttg ggtggcttgg
    13321 cccatcttct ttggttcagc cttcccttgg cagcttgctt ggctgttggg gcagcccttg
    13381 gtcctacgga cttggtggcc tttgcctctc tttcggagcg ttttagcttt cctaagcgcg
    13441 tgtccaatat tcttaagggc gaaggactct tgaatgatgc ttctggtttg gtggcttttc
    13501 aggtagcttt gacagcttgg acaactggag ctttttctct ggggcaagct agcagttcac
    13561 tcatcttttc aatcctaggc ggttttttaa ttggattttt aacagccatg accaaccgct
    13621 tcctccatac cttcttgcta agtgtgcgtg caacggatat tgccagtgaa cttttattag
    13681 aattgagttt gcctcttgtg accttctttc tggcagaaga agttcatgtt tcaggtatta
    13741 ttgccgtcgt agttgctgga attttaaagg caagtcgttt caagaaaatc acgctcctcg
    13801 aagcccaagt ggatacggtg accgagacgg tctggcatac agtgaccttt atgctcaacg
    13861 gttctgtctt tgtgatttta gggatggagc tggaaatgat agcagaacct atcttgacca
    13921 atccaatcta taatcctcta cttttattgc tatctcttat cgcccttacc tttgtcctct
    13981 ttgtcattcg ttttattatg atctatggct attatgccta tagaacccga cgcctcaaga
    14041 aaaagctaaa taagtatatg aaggacatgt ttctcttgac cttttcaggt gttaagggaa
    14101 cggtgtcgat tgctacgatt ctcttgatac caagtaatct agaacaggag tatcctctct
    14161 tgcttttcct tgttgcaggt gtgacgcttg ttagcttttt aacaggtctc ttggtcttgc
    14221 ctcatctttc tgatgaagag gaagaaagca aggattatct catgcatatc gccattttga
    14281 atgaagtaac gctagagttg gaaaaagagt tggaagacac cagaaataaa ctttccctct
    14341 atgcggctat tgacaattat catggacgta ttgaaaatct cattttaagc caagaaaacc
    14401 aggatgatca agaagactgg gctgctttga aactcttgat tcttagtatt gaaagtgatg
    14461 gtttggaaca ggcctatgaa gaggggaata ttagcaatcg tgcttaccga gtttaccaac
    14521 gttatctgaa aaatatagaa caaggaatca atcgtaaact tgcctcaaga ctgacctatt
    14581 attttcttgt ttccttgagg attttacgtt ttctccttca tgaagttttt actcttggaa
    14641 agaccttccg tagctggaag gacaaggagc aaagccgtct ccgtgctctt gattatgacc
    14701 aaattgcaga gctctatctt gccaatacag agatgattat tgaaagtttg gaaaacctga
    14761 agggagtcta cagacgctct ttgattagtt ttatgcagga gtctcgtctt cgagaaacag
    14821 ctattatcag cagtggtgcc tttgtcgaac gggttatcaa tcgtgtcaaa cccaacaata
    14881 tcgatgaaat gctgagaggc tattatctgg agcgcaagtt gattttcgaa tacgaagaaa
    14941 aacgattaat tacaactaag tatgccaaga aattacgaca aaatgtaaat aacttagaga
    15001 actattcctt gaaggaagct gccaataccc tgccgtatga tatggtggaa ttggtaagaa
    15061 gaaattagtt aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttggattat
    15121 atatgtgact gacttcgtca gtttcatcta caacctcaaa gcagggcttt gagcaacctg
    15181 cggctagctt cctagtttgc tctttgattt tcattgagta taagattata aatgaaggag
    15241 tatgacatga aaaagtggtg gaaagagctg atagacaagc ctttattaaa agcttttttg
    15301 cattattatc aagcatcaga tagtgagttg accagtgtcg cagtagccta ctattggttg
    15361 atttcgattt tccccctgct tttggtggtg gtcaatatcc tcccctattt tcagattcct
    15421 gtgggagaat ttctgtgctt tatgaaagac gttctgcccc caagcctcta tgaaggtgtg
    15481 gaaaaaatag ccagagaagt tttgacgcaa ccttcaacag gtttattgag tttttctgtt
    15541 ttatcggcgt tatggagttt ttcaaaatct atgaatttct tgcaaaaagc ttttaacaag
    15601 gcttatggcg tcgaaaagag tcgtggactt atttcccatc agatgctaag tctcctagtc
    15661 agttttggct tgcagctcct ctttgcgttt gccttgtttt tgatcttatt tggtcagatg
    15721 attttggctt tacttgctca ttattggaca aaagatggca ttatctatca ggctttacaa
    15781 gggttggcag gtcctctgat ttacgccctc ctctttgcta tcttggtcat gctttattat
    15841 tttcttccca atatatctaa caggaaaatt agctataccc taccaggcag tgcctttgtt
    15901 ctcttagtga ttttaggttt actaactctg ttctctagtt atctcaatta ctatgtccat
    15961 catttggtgg atgtccgaat tttaggttcc gttcttcttg ttgttatgat gttttggttt
    16021 atcttgattg ctaaaattgt tatcctaggc gcggttatca atgccagtat gcagagtttg
    16081 agagatccgg tctttaaaaa agactaacta tttatccatt acaaaagcgc atagtatcaa
    16141 aactttatag aaccttgata ctatgcgctt ttgtgctttg acagtttttt gtatagtttt
    16201 tgtccaagat ttctaaatca tctgattaaa ttcctatttt cccctatcat tgtccctgtt
    16261 ttttcgaaaa tggaaggagt ataataaatc tatcaataaa atgataggag gaactttatg
    16321 ggtcatattt tcttttttct aagtgtcttt ttggcaggga ttctatcctt cttttctcct
    16381 tgtatcttac ctttgttacc ggtctataca ggagtgttac tagatgataa ggatggtgct
    16441 caggcttcta gcggcaaatt ttcaatctca gttactagtt tattacgaac tctggccttt
    16501 atagcaggaa tttcctttat atttattttg ttgggctatg gagctggttt tttaggcgat
    16561 ttgctttatg cttcttggtt ccaatatctt actggggcaa ttattatcct tcttggtttg
    16621 caccaaatgg agattctaca ctttaagggg ctttataagg aaaagaggct acaactgcaa
    16681 ggacaggggc aaaatggtaa gggctatagt caggcatttt tattgggctt gacctttagt
    16741 tttgcttgga cgccttgcgt ggggccggtt ctggggtctg ttttggcctt ggcggcttca
    16801 ggtggttcag gagcttggca gggagctggt ctcatgttgg tgtatacgct gggcttggcg
    16861 ctaccattct tgcttctagc tctgacctct agttatgttt tgaaacattt ccgaaaactt
    16921 catccctatc tcggaatcct caaaaaagtg ggtggttttc tcattattgt gatgggactc
    16981 ttggttctgt ttggaaatgc ttcaatttta agtcaattat ttgaataaaa tggaaaggaa
    17041 tatcaatatg aaaaaatggc aaacatgtgt tcttggagca ggttcgctcc tttgtttgac
    17101 ggcttgttca ggcaagtccg tgactagtga acaccaaacg aaagatgaaa tgaagacgga
    17161 gcagacagct agtaaaacaa gcgcagctaa agggaaagag gtggctgatt ttgaattgat
    17221 gggagtagat ggcaagacct accgtttatc tgattacaag ggcaagaaag tctatctcaa
    17281 attctgggct tcttggtgtt ccatctgtct ggctagtctt ccagatacgg atgagattgc
    17341 taaagaagct ggtgatgact atgtggtctt gacagtagtg tcaccaggac ataagggaga
    17401 gcaatctgaa gcggacttta agaattggta taagggattg gattataaaa atctcccagt
    17461 cctagttgac ccatcaggca aacttttgga aacttatggt gtccgttctt acccaaccca
    17521 agcctttata gacaaagaag gcaagctggt caaaacacat ccaggattca tggaaaaaga
    17581 tgcaattttg caaactttga aggaattagc ctaggaggtg tcttatgaat gataagttaa
    17641 aaatcttctt gttgctagga gtattttttc tagccataac cggtttctat gttctattga
    17701 tacgaaatgc agggcagaca gatgcctcgc aaattgaaaa ggcggcagtt agccaaggag
    17761 gaaaagcagt gaaaaaaaca gaaattagtg aagacgcaga cttgcacgaa atttatctag
    17821 ctggaggttg tttctgggga gtggaggaat atttctcacg cgttcccggg gtgacggatg
    17881 ccgtttcagg ctatgcaaat ggtagaggag aaacaaccaa gtacgaatta attaaccaaa
    17941 caggtcatgc agaaaccgtc catgtcacct atgatgccaa gcaaatttct ctcaaggaaa
    18001 tcctgcttca ctatttccgc attatcaatc caaccagcaa aaataaacaa ggaaatgatg
    18061 tggggaccca gtaccgtact ggtgtttatt acacagatga caaggatttg gaagtgatta
    18121 accaagtctt tgatgaggtg gctaagaaat acgatcaacc tctagcagtt gaaaaggaaa
    18181 acttgaagaa ttttgtggtg gctgaggatt accatcaaga ctatctcaag aaaaatccaa
    18241 atggttactg ccatatcaat gttaatcagg cggcctatcc tgtcattgat gccagcaaat
    18301 atccaaaacc aagtgatgag gaattgaaaa agaccctgtc acctgaggag tatgcagtta
    18361 cccaggaaaa tcaaacagaa cgagctttct caaaccgtta ctgggataaa tttgaatccg
    18421 gtatctatgt ggatatagca actggggaac ctctcttttc atcaaaagac aaatttgagt
    18481 ctggttgtgg ctggcctagt tttacccaac ccatcagtcc agatgttgtc acctacaagg
    18541 aagataagtc ctacaatatg acgcgtatgg aagtgcggag ccgagtagga gattctcacc
    18601 ttgggcatgt ctttacggat ggtccacagg acaagggcgg cttacgttac tgtatcaata
    18661 gcctctctat ccgctttatt cccaaagacc aaatggaaga aaaaggctac gcttatttac
    18721 tagattatgt tgattaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac
    18781 cgtatgtagg gttactgact tcgtcagttt tatctacaac ctcaaagcta tactttgagc
    18841 tgacttcgtc agttttatct acaacctcaa agcggtactt tgagaaacct gtggctatct
    18901 tcctagttta ctttttgatt tttattgagt ataagaggtc tttcctaagc agttagagga
    18961 gaattttgct atactgatac tagtaagtgg taaaggagca gagcatgacc tacacaatct
    19021 taatcgtaga agatgaatat ctggtaagac aaggtttgac taaactggtc aatgtagcag
    19081 cctacgatat ggaaatcatc ggtcaggctg aaaatggaag gcaggcttgg gaattgatcc
    19141 aaaagcaggt gccagatatc attttaaccg atatcaacat gcctcatcta aatggcatcc
    19201 agttggccag tctggtacga gaaacctatc ctcaggttca tttggtcttt ttaacaggtt
    19261 acgatgattt tgattatgcc ttgtctgctg tcaaactagg tgtggacgac tacctgctca
    19321 aacccttttc tcgtcaggat attgaggaaa tgttggggaa aatcaaacaa aaactagaca
    19381 aggaagagaa agaagagcag ttacaagatt tattgactaa taggtttgaa ggaaacatgg
    19441 cccagaaaat ccagtctcat ctggctgata gccaatttag tttaaagtct ttagccagtg
    19501 acttaggttt tagtccgacc tatctgagtt ccttgattaa gaaagagttg ggcttgcctt
    19561 ttcaggatta tctggtgaga gaacgtgtta aacaagccaa gctcttgctt ttaactacag
    19621 atctgaagat ttatgagatc gcagagaagg ttggttttga agatatgaac tattttaccc
    19681 aacgttttaa gcagattgca ggtgtgacac ctcgtcagtt taagaaggga gaagaccgat
    19741 gaagcgttct tctcttttag ttagaatggt tatttccatc tttctggtct ttctcattct
    19801 cctagctctg gttggaactt tctactatca atcaagttct tcagccattg aggccaccat
    19861 tgagggcaac agccaaacga ccatcagcca gactagccac tttattcagt cttatatcaa
    19921 aaaactagaa accacctcga ctggtttgac ccagcagacg gatgttctgg cctatgctga
    19981 gaatcccagt caagacaagg tcgagggaat ccgagatttg tttttgacca tcttgaagtc
    20041 agataaggac ttgaaaactg ttgtgctggt gaccaaatct ggtcaggtca tttctacaga
    20101 tgacagtgtg cagatgaaaa cttcctctga tatgatggct gaggattggt accaaaaggc
    20161 cattcatcag ggagctatgc ctgttttgac tccagctcgt aaatcagata gtcagtgggt
    20221 catttctgtc actcaagaac ttgttgatgc aaagggagcc aatcttggtg tgcttcgttt
    20281 ggatatttct tatgaaactc tggaagccta tctcaatcaa ctccagttgg ggcagcaggg
    20341 ctttgccttc attatcaatg aaaaccatga atttgtctac catcctcaac acacagttta
    20401 tagttcgtct agcaaaatgg aggctatgaa accctacatc gatacaggtc agggttatac
    20461 tcctggtcac aaatcctacg tcagtcaaga gaagattgca ggaactgatt ggacggtgct
    20521 tggcgtgtca tcattggaaa agttagacca ggttcggagt cagctcttgt ggaccttgct
    20581 tggggccagt gtcacatctc ttcttgtctg tctctgctta gtgtggttca gtcttaaacg
    20641 ctggattgct cctttgaagg atttgagaga aaccatgttg gaaattgctt ctggtgctca
    20701 aaatcttcgt gccaaggaag ttggtgccta tgaactgaga gaagtaactc gccaatttaa
    20761 tgctatgttg gatcagattg atcagttgat ggtagctatt cgtagccagg aagaaacgac
    20821 ccgtcagtac caacttcaag ccctttcgag ccagattaat ccacatttcc tctataacac
    20881 tttggacacc atcatctgga tggctgaatt tcatgatagt cagcgagtgg tgcaggtgac
    20941 caagtccttg gcaacctatt tccgcttggc gctcaatcaa ggcaaggact tgatttgtct
    21001 ctctgacgaa atcaatcatg tccgccagta tctctttatc cagaaacaac gctatggaga
    21061 taagctggaa tacgaaatta atgaaaatgt tgcctttgat aatttagtct tacccaagct
    21121 ggtcctacaa ccccttgtag aaaatgctct ttaccatggc attaaggaaa aggaaggtca
    21181 gggccatatt aaactttctg tccagaaaca ggattcggga ttggtcatcc gtattgagga
    21241 tgatggcgtt ggcttccaag atgctggtga tagtagtcaa agtcaactca aacgtggggg
    21301 agttggtctt caaaatgtcg atcaacggct caaacttcat tttggagcca attaccatat
    21361 gaagattgat tctagacccc aaaaagggac gaaagttgaa atatatataa atagaataga
    21421 aactagctaa ctcccagtca attctgggag ttttatgcgt aattgggcaa actttctagg
    21481 ttgtctatct tgctaaaaac aagaaaaaac ggtatgatag aagagtgaca acagaggtaa
    21541 cagagatgaa cgagacacag attcaaagag aaacacgtca ggtagtggaa gatgttttag
    21601 aaaagaccaa tttgaagcag ggggcccttt ttgttttagg tctatcttct agtgaggtgc
    21661 taggtggtca tattgggaaa gaatccagtc aagagattgg tgagctgata gtggagacta
    21721 tcttggatat tctaggtagt agaggtatcc atttagcagt tcaaggttgt gagcatgtca
    21781 atcgggctct cgttgttgaa cgtcaggtgg cagagcagtt tggtctggaa attgtcagtg
    21841 tccatcctac tcttcatgca ggaggttcgg ggcagttggc agcctttaag tttatgcagg
    21901 atccagttga ggttgaattt atcaaggctc atgctggatt ggatatcgga gacactgcaa
    21961 ttggcatgca tgtcaagcat gttcaggttc cgattcgccc tattttgaga gagattggtc
    22021 atgcccatgt aacggcactg gctagtcgtc caaaattaat cggaggtgcg cgtgcgcact
    22081 atccgcaaga tgctattaga aagacctgag aatgaataaa ggagataaaa gtgtttaaaa
    22141 aagaccgttt ttcaattcgt aagattaagg gagttgtagg ctctgtattt cttggaagcc
    22201 ttttgatggc tccttctgta gtggacgcag ccacctatca ctatgtaaat aaagagatta
    22261 tttcacaaga agctaaagat ttaattcaga caggaaagcc tgacaggaat gaagttgtat
    22321 atggtttggt gtatcaaaaa gatcagttgc ctcaaacagg gacagaagca tctgttttga
    22381 cagcttttgg ttatctatca ggtgacatcc tcaaaacgct tggcttggac actgttttag
    22441 aagaaacctc agcaaaacct ggagaggtga ctgtagtcga agttgagact cctcaatcaa
    22501 caacaaatca ggagcaagct aggacagaaa accaagtagt agagacagag gaagctctaa
    22561 aagaagaagc acctaaaaca gaagaaagtc caaaggaaga accaaaatcg gagataaaac
    22621 ctactgacga cacccttcct aaagtagaag aggggaaaga agattcagca gaaccagctc
    22681 cagttgaaga agtaggtgga gaagttgagt caaaaccaga ggaaaaagta gcagttaagc
    22741 cagaaagtca accatcagac aaaccaacag aggaaccaaa agttgaacaa gtaggtgaac
    22801 cagtcgagcc aagtgaagac gaacaggcac caacggcacc agttgagcca gaaaagcaac
    22861 cagaagctcc tgaagaagag aaggctgtag aggaaacacc gaaaccagaa gataaaataa
    22921 agggtattgg tactaaagaa ccagttgata aaagtgagtt aaataatcaa attgataaag
    22981 ctagttcagt ttctcctact gattattcta cagcaagtta caatgatctt ggacctgttt
    23041 tagaaactgc aaaaggtgtc tatgcttcag agcctgtaaa acagcctgag gtaaatagtg
    23101 agacaaataa acttaaaacg gctattgacg ctctaaacgt tgataaaact gaattaaaca
    23161 atacgattgc agatgcaaaa acaaaggtaa aagaacatta cagtgataga agttggcaaa
    23221 acctccaaac tggggttaca gaagctgaaa aagttgcagc taatacagat gctaaacaaa
    23281 gtgaagtaaa ttctgagact gcaagtctta aaacagcaat ttctagatta aatacagata
    23341 aagttgaact cgaaaatcaa cttaagattg cacagggaaa aactgaaact gatttcagca
    23401 tggaatcttg gactgttttg agtacagcta aaaataaagc tcaggaagta aaagataacg
    23461 gaacagcgac ccaagaacaa ataaacgaag cagagaaaag tttgaaaaca gcccttgctg
    23521 acttatctgt tgacaaaaca gctttgggaa gcgctatcga tacagctact aagaaaaata
    23581 aagagaatta tacaaatcaa acatgggcag agttggaaac agcgcttaca gcagcaaaat
    23641 ctgtaaatac taacgagtct aaacaaagcg acgttaacga agctgctgaa aaattaactg
    23701 caactatgga aaaattggtt gaattgtctg aaaagccacg tttgactcta tctattgaaa
    23761 agagagacat agatcgaaaa gctacggtca cgtatacttt ggaaaatcca gcaaatactc
    23821 aaataaaatc aataactgca actctgaaaa aaggagagga agttgttaag gattttgttt
    23881 taacagaaga aaatcttaaa actaatcatt taactgcttt gtttgaaaag ctagactact
    23941 acaaagaata cactctatct acggatatgg tatataacag aggaaatgat gatgagacgg
    24001 agagtatttc agaagaacta attcagctga atcttaagaa actagaattg aaagatatcc
    24061 agacagtctc tctgatgaaa tttgaaaatg gtcaagaaag tcaggttaca cacttgtctg
    24121 ataaaccgac cgatttgagt aaattatact taaaagttac atcatcaacg tctaaggatg
    24181 ctgtgttagc cgttagctct atagaagaag agattattga aaataaaaaa atctttaaaa
    24241 ttcatgcaga tacaccagaa ttagtagttc gtaagaaaga tggcagtctt tctaagggat
    24301 ttgactatta catggagaga gttatccctc atgatggtga tatttattat gattttaaag
    24361 acttgatttc agctatgaca tctaatccta caggtacgtt tattttaggg agagatattt
    24421 cctcgcgtaa tgtaaaaccg gatggaaatg gaaaatctta tatcaaaggt gaatttaaag
    24481 gaaaactact tggtacaaat gataatgtta gacactcgat ctttgactta gaatatccat
    24541 tgtttgatac tataaaatca ggagtcgtga aagatattga tttcaagcat gtcaatatgg
    24601 tattcccaga tagcaatcaa ggagataacg tagcaacaat tgcaagagta atcaaagata
    24661 agacaaaaat tgagaatgtt aatgttgaag gttatctcga gggtcgagat catgtagctg
    24721 gtttagttaa taatcttgaa ggtaattccg aaattgaaaa tgtatccttt acaggaaaga
    24781 ttaagtctaa aggtggtaat tctattacag caggtattgc aggtagaaat atcttatctc
    24841 gtgttaaacg tgcctatgta aatgcagata tagaggtaca tcgatcatct aattctagca
    24901 tgcttgttgc agtaaatgga ataaacgcag atgcaagtgg tggttgggga acctggggta
    24961 gacttactga gtcagttgct aaaggaacat tagaaaccaa gcaaggaggg caagctggag
    25021 gagcctcttc tacagtctgg ccttatggag caattgataa tgttgtcagc tatgcaaaag
    25081 tgaccaaagg gaaagaatta tttggatcag atggtgattt aaattatgat tggtttatga
    25141 aaaaaattag taatattttt ggtgttcaag gcatcagctc aggcgattcg ggtagtgatt
    25201 ctaaatttac gcgtatttct gaggaagaag caaatcaaaa agttgcttct tataatatta
    25261 cagctcccaa cttgatgagt gactcaagtc ttttggtaga cagactcaat gaaagttgga
    25321 aaaatactga tcaatttgaa agtatacagg attatcaagc ccaaaaccaa ttaatttacc
    25381 agaacttgac taaatttact ccttattaca ataaagaatt tattgttcat gaaggaaatg
    25441 ctctcactcc tgaacaggaa atattgaaga ctaagaaaat caaatcgatt gtagggttga
    25501 aaggaacaga gttcgttgta gatggcagtg atattgatac tatcatgctt cattttgagg
    25561 atggtagtca gaagcgatat aaggtaacct ctactggtaa gttttcgata actaatttac
    25621 cggaatatca ggttgaagat ttaaatgtgg tgtatacatc agaacatata gtacatccac
    25681 tagattcgtc actgataaat aaccttgttg aagaattgaa aaaagtagaa ttgtatacag
    25741 agtctactta ccaagttcta ggtattgata aagataatgc taacaaattg aatagaacta
    25801 agagattatt cttagatgag tcattagatg ctgttaaaac acaattgcct acctttgtaa
    25861 aaacaatgtt tgaaaatgag tggttgcaca tcaatggaga atctagtgga gctgtcgctg
    25921 ctttacgaca aaaaatcatg gataataaga cggctatatt gcttgctttg acttatatca
    25981 atcgttatta tgatgttaaa ttctcagact ataatattaa aaaactgatg ctctttaaac
    26041 caactttcca tggtgaaaag attgatctat tagatcgatt aatccgacta ggatcatctg
    26101 gagaaaatag attaaaaggt tcagaaaatg cagaaacgtt caagcagtta tttgctagcg
    26161 aaacgaagca gaaggatttg gtgacttatt tagactacaa tcgctctttg ttgactaatt
    26221 atcaaacaac aggggaatgg tttaaagaaa cgactaagga ttatattcaa tttgaggaac
    26281 gtccatcctt agtagaggag attaaagatg ccaaatatcg agtttacgat aatttaaccg
    26341 caccgtatta tcaaggatat atcctacctc tcttaacgtt gaagaatact catttagcaa
    26401 ttttatcgaa ctatagtact atgacatttg tcagccgtga aaaacgccct aattggaaaa
    26461 atgaagattt tgataagtgg gtgaaatatg tagcaactgc tcatagaaac catgttgata
    26521 cttggtataa gattttacct gataatatca aaggtaaaat ggttaaagag aatgttactg
    26581 ctgtttggga aggattgagc atcccaggaa gtgaatgggt agaccagaat gctgtagata
    26641 gaaaaggaag agattacgcc ccagctcgag aattctttaa tcttgtaggt ggtcctatgg
    26701 gaggttggta tgcttaccat ggttatggtg cgcatgctgg aggaagaaat cgggttaatt
    26761 atgaggtgtt tgatgtatta agtgagtatg gtatatcggt ctttactcat gaattgactc
    26821 acgttaatga tacttggatt tacttaggag ggtatggtag acgtgaaaat atgggacctg
    26881 aagcttatgc tcagggatta ttccagtctc ctgttccagg tcagccgggt tggggggcgc
    26941 ttggtctcaa tatggcattt gaaagaaaaa atgatggtga tctaatttat aatgcctccc
    27001 caactcaatt tgaaaatagg aaagagttag atagttatat gaaaaattat aacgatactc
    27061 tcatgatggt ggactacttg gaaggagatg cagttatctc taaaggtaaa gaagctatta
    27121 ctaaatggtt taaaaaagtt gaacctaagg ttgttagtca aacagctcag tatgatacgg
    27181 ttcgtcagtt gactgctgaa gagaaagaaa aactttctgt ttctagtgtt gatgatttag
    27241 ttgatcaagg attaatgtca gacagagctg taggaaataa tacctataat ccagctgatt
    27301 ttgaaacaag ttatattgcg atagattata tgactggtat ttatggtgga ggaaagaata
    27361 gtgtaggttc accaggtgcc ttgatgttta aacataatac tttccgtatg tggggctact
    27421 atggttttga agaaggtgtt ctaggatatg cttctaataa atttaagcaa gcatctagag
    27481 atgaaggtca tgctggactt agtgataact ttattatttc taagatttcc aaaggggaat
    27541 ttttaactat ggaagcattt aaaaagggat attttaagaa agtagtagaa gagttaaaaa
    27601 ctaaaggtat tcgtcctgtt actatcaatc aaaaaacata tagcaccttt gaagaattgc
    27661 aagaaggatt taagcaggct gttgaaagag atttgaagaa aaatcagtta gatgagaggg
    27721 aaacaagaaa cttcaaattc caagttttca gacaacttct tcaacaaaca gatagcttta
    27781 aaacttctat ctttagataa tcgaaatctc tcatctgctt tgcggatgag ggttttctta
    27841 ttttatgcta tacttaaagt atgcatagaa aaacagtgat tgattttagg actttggggg
    27901 agagatatac ctttacccag cctattaaag agttgaaaac gagaaatgta gaagaagtgg
    27961 cagatttgct ggcacaagtg gaaagctacc aagagcaagg ttattatgtg gtggggtatg
    28021 tcagctacga ggctgcacct gcttttgagg aaaaattagc agttcacaag gatcccctac
    28081 tgggagagta cctgctttat tttactgttc atgatagcgt agaaacttcc cctattcctc
    28141 tgacttatga ggatattgat ttgccctcaa attggcagga agtaacgtct gcagcagact
    28201 atgaaaaggc cattgcccaa attcaccatc atttacggca gggagatacc tatcaggtca
    28261 actacaccgt ccaacttaag caaaagttaa atgccaatcc ttttgccatc tacaatcgta
    28321 tggtggtaga gcaggaggcg ggctacaatg cctatgtgga acatgacgag atggcagtga
    28381 tttccatgag cccagagctc ttttttgagc aaaatgatcg cgagttgaca acacgaccaa
    28441 tgaaggggac gactcagcgt ggggtaactg accaagaaga tcttgaacag gccagttggt
    28501 tggaacagga tcccaaaaat cgctctgaaa atatgatgat tgtggacctc ttgcgcaatg
    28561 atatgaaccg tatttctgaa gttgggagcg agcacgtgga gcgtctgtgt caggtagagc
    28621 agtattcaac tgtttggcag atgacttcga ccatcaagag tcagttgcga gaggatgtgg
    28681 accttgttga aatcttccac tcactctttc cttgtggttc cataacgggt gcaccgaaaa
    28741 ttgcgacaat ggagattatc aaggacttgg agcctcaacc gcgtggagtc tactgtggaa
    28801 cgattggtct cttgcttcca aatggacgac ggatttttaa tgtggccatt cgtaccattc
    28861 aacttcacaa aggtcaagcc atttatggag ttggcggagg gattacttgg gatagcactt
    28921 gggaatctga ataccgtgaa gttcatcaaa aggcagcagt tctctatcgt aaacaagctc
    28981 gcttccaatt aattacaact ggaaaaatca gccaaaaaca actgatgttt gaagaacaac
    29041 atctggaaag gctgagaaaa gctagtcggt attttgctta tccttttgac ccagaaattt
    29101 tgagacaaaa gattgaagca gagtgtcaga cttgtgatgc taatcaagat taccgcttgc
    29161 gaatttctct cagcaagtct ggagagatag aattcagtcg tcaaatctta acacctctta
    29221 gtccaagttt ttgtcaggcc aaactttgtc tgcaagaagc gaatttgaat caatccttta
    29281 cctattttaa aacgacttac agaccgcatt tgagcataga ggaacaagag aagatttacc
    29341 ataatggaaa aggagaactt cttgaaacct ctatcggaaa tttggttctg aaaatcgatg
    29401 ggaaactcta cacaccgcct atccgacttg gaatcttgcc aggaatttat caccagtatt
    29461 tactagaaac aggacaggta gaagagaaag tcttgacctt ggcagatttt gcccaagcag
    29521 aagctattta cggctgtaat gcagtgagag gcttgtatga gttgcaagtg ataggattgt
    29581 gagttttagc tattaatagt ttttaatata tattaaaata tattggtgtt tacttttgat
    29641 gagatatcat atttgtgtta taatgtaacc gtaaacaata gaaatttaaa ggagattttt
    29701 gatgaaatca aaaatagttt tgggagcttc gctagcgatc gcaacactta gcttagtatc
    29761 tcttgtcgag attgaaggac tctcaccgtt tttaatagag aatgttagcg caaatactca
    29821 ttctgcaaat aaagttatta atcataaagt aagtatttac ttagaaaatg ctgatgaagg
    29881 taaaggtcta actgtaaact tttcaacaga cagtgttagt ccaaatttat ttgatgagtt
    29941 cgaaaaaaag agtggaatta caataactac tatgttagtg aatgctaaga caggtgaagt
    30001 tgtagaaaaa agattaacac caagcgtttt tttacgttcc aatgatttaa ccagtgggac
    30061 catatcttcg tttattttta gtgaatatcc tgatggagaa tataaatatg ttgtgtctaa
    30121 aggagacttt atcgatccta agactcagtt taaacatcag tatagaggag aatctccagt
    30181 ttttagaata cgtaatcgaa aatatgtgga gttgggaaca actgataaaa aattagatga
    30241 aagacgtgat aatagcgttt ataaagatgg ggttgttgaa cacaaggtaa atttaagttt
    30301 aacaagttat caaggaggta atggagttac tgcaattttt tcaactgata gtgttaactc
    30361 taacttactt aattcttttg gtgaaaaggc taaaaaagtt ttgattaggt ctaagttaat
    30421 taatgttaag actggcgagg ttattgatga aacgttttct ccaaaagtaa gtttaacctc
    30481 taaaattttg aaaagtggtt ctacagcagt attttatttt atagatttaa ctgatggcga
    30541 atataaatat gttgcgtatg aaagccagca atatactgat cctcaaacaa cattgactca
    30601 tcagtataga ggagaatctc ctatttttag tatcaaagat ggaaaatttt ctggtttagt
    30661 atctgcctca aaaccagacg aaaacccgaa gccaacgcca aaaccggatg agaaaccaaa
    30721 accttcagca ccacaacagg agaaaacaaa acctacagtt cagtctggtt gggtaggttc
    30781 atcttattat caaaatggta aaaaagtcac tagtaagtgg atttttgata aaaaatataa
    30841 ttcatacttc tatctagatg catcaggaaa ttatgttcaa aacgcttggg taggtaacta
    30901 ttatctcaaa tcaggcggat acatggctaa aagtgagtgg atttatgata agaactatgg
    30961 ttcatactac tatctaacat cagaaggcag ctacgctcgt aacacttggt caggcaatta
    31021 ttatttgaag tcaaatggta agatggctaa aagtgagtgg gtatacgata gtaactacaa
    31081 atcatattac tacttgacat cagaaggaag ttatgctcgt aacacttggg taggcaatta
    31141 ctatcttaaa tcaaacggta aaatggctgt taatgaacgt acaccagatg gttatcgtgt
    31201 agatggttca ggtaaatggg ttaaataaga aactaaaaaa taggataata ggtaatatca
    31261 gatgaataaa aaaaattatt ttcatgtgct ttagcatttt caacgattgg aacattgtca
    31321 acgattgata aatcaggagt tcttcctttt tcagcgcaaa cggtgcaggc tgacatacaa
    31381 acgcttaccc caatggcaaa atattatatt attcttccga aggatgcaga aatatataaa
    31441 acttggagag gtactgtcaa tattcccatt attgatgcta caaaaactac tccagaatta
    31501 tcttatttca aagaagatca tcgcaactat atagctaatg agaataagtc tggtgccaat
    31561 tacatagagt ggaagggaac agtagaagag tttaaagagg ccattaagaa gttgactgat
    31621 aaaaaatcaa ctactgcaac accaaaaaag gatgaaaagc caacaccaaa accggatgag
    31681 aagccaaaac caacacctac agttcagtct ggttgggtag gttcatccta ttatcaagat
    31741 ggtaaaaaag tcattagtaa gtggattttt gataaaaaat ataattcata cttctatcta
    31801 gatgcatcag gaaattatgt tcaaaacgct tgggtaggta actattatct taaatcaggc
    31861 ggatacatgg ctaaaggtga atgggtttat gatgccacct atcaagcttg gtattatttg
    31921 acatcagatg gttcttatgc ttacagtaca tggcaaggaa attactatct aaaatcggat
    31981 ggtaaaatgg ctgtcaatga atgggttgat ggtggacgtt attatgttgg cgctgacgga
    32041 gtttggaagg aaggtcaagc aagtacagct tcttctagta atgatagcaa tagtgaatat
    32101 tctgctgctt taggaaaggc aaaaagttat aattcgttat tccacatgtc aaaaaaaaac
    32161 gtatgtatag acaattaact tctgattttg ataaattttc aaatgatgca gctcaatatg
    32221 ccattgatca ttagatgatt aacagatact ggtaggcgaa aaaattcctt ataatccatt
    32281 tacgcaaacc tttgcataag tgaatggatt ttgatatact agtactgtaa gcattttcaa
    32341 ttactacaca atgaaaaagg agaatatgat gagtcaaaag attattggga ttgaccttgg
    32401 tggaacttct atcaaatttg caatcttaac aacagcagga gaaatccaag gaaaatggtc
    32461 aatcaagacc aacattttgg atgagggaag tcatatcgtt gatgatatga ttgagtctat
    32521 tcaacatcgt ttggacttgc ttggattggc agcagcggac ttccaaggca ttggaatggg
    32581 atcaccaggt gtggttgacc gtgacaaagg gactgttatc ggtgcctaca acttgaactg
    32641 gaaaaccctt caaccaatca aacaaaagat tgaaaaagct ttgggcattc catttttcat
    32701 cgataatgat gccaacgtag cagctcttgg tgagcgctgg atgggtgctg gagataacca
    32761 accagacgtt gtctttatga cactcggtac tggtgttggt ggcggtatcg tcgcagaagg
    32821 caaattgctt cacggtgttg ctggtgcagc aggtgagctt ggtcacatca ctgttgactt
    32881 tgaccagcca atctcatgta cttgcggtaa gaaaggctgc cttgagacag ttgcttcagc
    32941 aacagggatt gtcaacttga ctcgtcgcta tgccgatgaa tacgaaggcg atgcagcctt
    33001 gaaacgcttg attgataatg gagaagaagt aactgctaag actgtctttg atctcgcaaa
    33061 agaaggagac gaccttgctt tgattgttta ccgtaacttc tcacgttact tgggaatcgc
    33121 ttgtgctaac attggttcaa tcctaaaccc atcaacaatc gtcatcggtg gtggtgtgtc
    33181 agctgcggga gaattccttc tacaaggtgt tcaaaaagtt tacgatgaaa atagtttccc
    33241 acaagtacgc acatctacta aattagctct tgcaactcta ggaaatgacg ctggagttat
    33301 cggagcagca tcacttgtat tgcaataaga taatgagaga ggaaaatgct aacctagagt
    33361 tagtaatgtt cctcttttat tttttatgct atactagtta ggacaatgaa tgaggtgaga
    33421 gtaaatgaca aaagcagata cgatttttaa agagaatatt gaatgcattc taaaggaagg
    33481 tgttttctca gagcaagctc gtcctaagta caaggatggg actgttgcta attccaagta
    33541 tgtaactggt gccttttccg agtacgactt atctaaagga gaatttccta tcacaacctt
    33601 gcgtcccatt gcaatcaaat ccgccatcaa agaagttctc tggatttacc aagaccagtc
    33661 aaatagctta gaagtgctta atgacaagta caatgttcac tactggaatg actgggaagt
    33721 tggagacacg ggaaccattg gtgagcgcta tggtgccgtt gttaagaaac acgacattat
    33781 caataagctt ctcaaacagt tggaaaccaa tccttggaat cgccgcaata ttatttcgct
    33841 ctgggattac caagctttcg aagaaacaga tgggctgctc ccgtgcgcct ttcagaccat
    33901 gtttgatgtc cggcgtgttg atggggaaat ctatctggat gcgaccttga cccagcgctc
    33961 caatgatatg ctggtggccc accacatcaa cgctatgcag tatgtggctt tacagatgat
    34021 gattgccaaa cattttggtt ggaaggttgg gaagttcttc tatttcatca acaacctcca
    34081 tatctatgat aatcaatttg aacaagctca ggaattgctc cgtcgggagc cgtcaaactg
    34141 ccaaccacgc ttggttttaa atgttcctga tgggactaat ttctttgata tcaaagcaga
    34201 agattttgag ttggtggatt atgaccctgt taagccacag ttgaagtttg acctagctat
    34261 ttaaaagaat agaaaaaaga agttgagaat aatcccaact tcttttgttt cttaacgtga
    34321 tacgcggcga cgagctgctt ttttacgatt ttcttcgatg aaagctgctt tttgctcttc
    34381 tggttcgatt actttctttt taaatgcgta tactgcacct gcaacggcag cgacagttcc
    34441 tgcgacacct gttacaagac ctttagcgaa tcctttagcc atgagtcttc ctcctttata
    34501 ttctcaatca gccagcctcc tcaagaggtc acatttttct gactgacctt tttgtgttat
    34561 aataatagta acgaaaaaat gggaattttt caaggaaaaa agatgaaaac aaaaataatt
    34621 gtgattgttg gaccgactgc tgttggaaag acggctctag ctattgaagt tgcaaagcgc
    34681 tttaatggcg aagtggttag tggagatagc cagcaagtct atcgaggact tgatattggg
    34741 acggccaagg ctagtccaga agagcaggca gctgttcctc atcatttaat cgatgttaga
    34801 gagataaccg agtcttactc ggcttttgat tttgtttcag aagctaagat gactattgag
    34861 gatattcaga gccgtggcaa gctagccatt atcgccggtg ggactggact ttatatccag
    34921 agcttgctag aaggttacca cctaggtggg gagactcctc atgaggagat tttagcttat
    34981 cgagctagtt tggagccata ttcagatgag gaattagccc atttggtgga gcaagcaggc
    35041 cttgagattc cccagtttaa tcgtcgtcgt gctatgcgtg ccttagaaat tgcccatttt
    35101 ggtcaggatt tggaaaatca agagatcttg tatgaaccgc tgattatctg cttggatgat
    35161 gaacgtagtc aactttatga gcgtatcaac caccgagtgg atttgatgtt tgaggctggg
    35221 cttttggatg aggccaagtg gctgtttgac cattccccta atgtacaggc tgctaaagga
    35281 attggctata aggaactctt tccttatttc cgtggagagc agacctttga ggaagctcgt
    35341 gagagtctta aacaggcgac ccgtcgtttt gccaagcgtc agctgacctg gttccgtaat
    35401 cgcatgcagg tcacctttta tcagattgga gaatctggtg tacaagaccg cattttaagc
    35461 cagatagagg agtttttaga tgattgaaac ggagaaaaaa gaggagcgag tcctgctgat
    35521 tggtgtggaa ttgcagggta tggacagttt tgacctctcc atggaagaat tggctagttt
    35581 agcgaaaacg gcaggggcag tcgttgtaga tagctacaga caaaaacgtg aaaaatatga
    35641 ttccaagacc ttcgtcggct ctggtaagtt ggaagagatt gcgcttatgg tggatgcaga
    35701 agaaatcact actgtcatcg tcaacaatcg tctgacccca aggcagaatg tcaatctaga
    35761 ggaagttctc ggtgttaagg tcattgaccg tatgcagttg attttggata tctttgccat
    35821 gcgggctcga agccatgaag ggaagctcca agtccaccta gcccaactca aatacctctt
    35881 gcctcgcttg gttggtcagg ggattatgct cagccgtcag gcagggggaa ttggttcccg
    35941 tggtcctggt gaaagccaac tggagctgaa ccgtcgtagc gttcgcaatc aaatcacgga
    36001 tatcgagcgc cagcttaagg tggttgagaa aaatcgtgcg actgtcagag aaaaacgttt
    36061 ggagtctagc acttttaaga ttggtttgat tggttatact aatgctggga aatcaactat
    36121 catgaacatc ttgaccagta agacccagta tgaagcagat gagctctttg cgactctgga
    36181 tgcgacaacc aagagtattc atctgggagg caatctccaa gtaactttga cagataccgt
    36241 tggctttatc caagatttgc cgacagagtt ggtgtccagt ttcaagtcaa ccttggaaga
    36301 aagcaagcat gtggaccttc tggttcatgt tatcgatgct agcaatcctt accacgagga
    36361 gcatgaaaaa acggttctct ccatcatgaa agacctggac atggaagata ttcctcactt
    36421 gacgctttat aataaagcgg atttggtgga ggatttcacg cctacccaaa cgccatatac
    36481 cctcatttct gccaagtctg aggacagtcg tgaaaacttg caagcattat tgctagataa
    36541 gattaaggaa atttttgaag catttaccct gcgagtgcct ttttcaaagt cctacaagat
    36601 tcatgattta gagagtgttg caattctgga agaacgtgat tatcaggaag acggcgaagt
    36661 gattacaggc tacatttccg agaaaaataa atggaggtta gaagaatttt atgactgata
    36721 ttaaaacttt ggctctaaag tatggaggtt atacaagtct ggacaaggtc tatctggatc
    36781 agcttctagc tggtaaaaca gatcaggagc agttaacact tattacacct ccgccaagtg
    36841 ttgttaatgc ctactttgca gaactctatc agaaaaagag tcctcaggct gcgacggatt
    36901 attttgcaga actcagtcag gaactgaatc tttacaatac tgagccaagt ttcaccctag
    36961 aaaataagcc ttttattcgg cttaatctgt ctggtaaatc ctttggtttt tgttatgaga
    37021 gtgacggcct tggtcggatt ttctttgaaa atgaggagaa aatctcggat gacttgctct
    37081 ttgaaattgc gcaaattttc ccccatcaat tagtctttga agagtctggt aagatttaca
    37141 tgaagcctgt cgaggacgag gaagttatca gcctagaaag tctcacagct ttgactgatt
    37201 tggaaagatt ggctgatggt cgaaaacgtc tcaaaggcta cagccaagag gatttattgc
    37261 aagaagctgc tgctttttct ggcaaacgct atttccgatc ggaaaaccgc acagccatgt
    37321 tatatattga ttaattagaa agtatcgaat ggatattcaa tttttaggaa cgggggctgg
    37381 tcagccctct aaagcccgca acgtttcaag tctcgccctg aaacttttgg atgagattaa
    37441 cgaagtttgg ctctttgact gtggagaagg tacgcaaaat cgcattctgg aaaccacaat
    37501 tcgaccacgt aaggtcagca aaatctttat tacccatctg catggagacc acatttttgg
    37561 tttgccaggt ttcctttcta gccgtgcctt tcaggccaat gaagagcaga cagatttgga
    37621 aatctacgga cctcaaggaa tcaagtcatt tgtcttaacc agccttcgtg tgtcaggttc
    37681 tcgtctgccc taccgcattc atttccatga gtttgaccaa gattctctgg gtaaaattct
    37741 tgaaaccgat aaattcactg tgtatgcaga ggagctggac cacactattt tctgtgttgg
    37801 ctatcgtgtc atgcaaaagg atctagaagg gacgctggat gctgaaaaac tcaaggctgc
    37861 tggtgttccg ttcggcccgc tttttggtaa aatcaaaaac ggccaggatc ttgttttgga
    37921 agacggaact gaaatcaagg cagcagacta tatctcagcg ccacgtccag gtaagattat
    37981 cactatttta ggagacactc gaaaaacggg tgccagtgtg cgtctggctg tcaatgcaga
    38041 tgtcctagtt catgagtcca cttatggcaa gggtgatgaa aaaattgctc gtaaccatgg
    38101 tcactcaact aatatgcaag ctgcacaagt agcggtagaa gcaggtgcca aacgcctcct
    38161 actcaaccat atcagtgccc gtttcctctc aaaagatatt agcaaactca agaaagacgc
    38221 tgccacaatt tttgaaaatg tccatgtggt caaagacttg gaagaagtgg aaatctagca
    38281 gtcacagaaa ggataagtat gcctactatt ctcattaccg gagctagcgg tggtctagct
    38341 caagaaatgg tcaaactcct gcccaatgac caactcatct tgcttggtag aaataaggaa
    38401 aaattagccc aactctacgg aaattattcc catgcagaat tgattgaaat tgatattacc
    38461 gacgattcag ccctagaagc tctggtaact gatctttatc tccgctatgg caagattgat
    38521 gtcttgatta acaacgctgg ttacgggatt tttgagggat ttgaccagat tgctgataaa
    38581 gatattcacc agatgtttga ggtcaatacc tttgccctga tgaatctgtc tcgtcacctt
    38641 gcggctcgta tgaaggaaag cagcaaaggg catatcatca acatcgtcag catggcaggt
    38701 ctaatagcta ctggcaagtc tagtctttac tcagcgacca agtttgcggc tattggtttt
    38761 tcaaatgctc tgcgactcga acttatgccc tatggagtct atgtgacaac agtcaatcca
    38821 ggtccaatcc gaacaggatt tttcgaccaa gccgacccag atggaactta tcttaaatcg
    38881 gttgaccgct tcctgctaga accagatgca gtggctaaaa agattgtcaa gattatagga
    38941 aaaaacaaac gagaactcaa tctcccgatt ttgttgaacc tagctcataa gttttatacc
    39001 ctctttccta agctagctga taagttggca ggggaaactt ttaattataa gtaaaaagaa
    39061 ccaatgtgca ggttgttgct agcctacata ttggttctta tcatttctca actatcaaac
    39121 tgaacttctt ctaggagata gtctataaag cgttcgccca tcttagatag gctggttttc
    39181 tcatgctgga tatagaccag ctcgatcggg tcatcaatat ccagtgggat agaaacgata
    39241 ttgtctccgt ttaggttgct gttcaaaatc cctgtcgcaa tggtataacc atccaaacca
    39301 atcaagagat taaagagggt ggcacggtca ctgaccacaa tggatttctt gtggtgttct
    39361 tgagaaagaa tctcttctga aaagtagaag gagttgtgcg tcccttggtc atagctgagg
    39421 taagggaaat tctccaaatc agacagtttc accttgtctt tctttgccag agggttggtc
    39481 ttgctgacaa agatatgtgg ttgcgctgtg aagagatggt gggctagcag gtgattgtca
    39541 tccagcatct tggttaaaac atcacggttg taactgttta agaagaggac cccgacctca
    39601 ctgcggaagt tcttgacgtc gtcgataatc tcccaagtcc gagtttcacg aaggaagagt
    39661 tcgtatttct ccatatcgct tttcttgagc aaagagacaa aggcattgac cacaaaggca
    39721 tagtgttgag acgaaacgct aaatagttcg cggtgggcga caggattttt atagcgttcc
    39781 tccagaagct gggtctgctc gacaacctga cgggcataag agagaaactc catcccatca
    39841 cgggtcaagg tgattccctt gggattgcgg ataaagatct caatgcccat ttcattttcc
    39901 aaatctcgca ctgcattgga gagacttggc tgagtgataa agagttgctt ggctgcctca
    39961 ttcatggagc cagtttcgac gattttgata atataatgta attgttgaat tctcatgttt
    40021 ttattgtacc atacttgcct tagaatcgct aaagaagata ggaaaataag gagcattgtg
    40081 tgagtctctt cttgaaaaaa acacaaaatt ttgctagaat gagtcttatg aaaacattct
    40141 atgatgtgca gcaattcctc aaacgatttg gtattattgt ttacatggga aaacgcttat
    40201 atgatattga actgatgaag ttggaactat ctcggattta cgatgcaggg ttgatggata
    40261 aattagacta tctagaggca gaagcagttc ttcgcagaga gcacaaggta gaattggatt
    40321 atattgagaa aaatggagaa aagaactaat ggttacttgg attttgtggg cacttatact
    40381 agcaatgttg gcgtggatgg gctttaacta tcttcgtatt cgccgtgcgg ctaaaattgt
    40441 ggacaatgag gagtttgaag ccttgattcg tacgggtcaa ttgattgatt tgcgcgaccc
    40501 agcagaattc cacagaaaac atatccttgg tgcacgcaat attccttcaa gtcagttgaa
    40561 aactagtctt gcagcccttc gtaaagataa acctgtcctt ctctacgaga accaacgtgc
    40621 gcaacgagtt acaaatgcag ctctttactt gaaaaaacaa ggtttttctg agatttatat
    40681 cctttcttat ggcttggatt cttggaaagg gaaagtgaag actagctaac cttatttcca
    40741 aattagtcga atgataggat gaagctattg ttttatagta taattaattt gtataaaatt
    40801 taaggagttc ttagaacata aaagaaaaga agaaaaatcc actttttgtg ggtatcttgt
    40861 cgattattct tggtttgtta tttccaattg taggcctgat tttgggtatc ataggattgg
    40921 tcttggctat ttcataccaa aaagagtcat aattagacta taaaatagaa aagattctta
    40981 atatcttagg aattgtgatt tctgtagtta actggattgt agcaatcgcc ttaatttttc
    41041 ggtaaataaa agaaaaaagc tttagtttta aagctttttt tgttttatcg actcatctct
    41101 aaatagtttt taatgatttg taactctttt tggttcaaag ggcggtagtt actttctgct
    41161 aaatctgggt tcaatgtgaa gtccccaaat tggattctct tgaggatagt caccttaaca
    41221 ccaaccgaga ggaacatttt cttgatttga tgaaattttc cttctgaaat ggtgatagag
    41281 gcttgactga tggagggact tgcagataga atctctagtt ttgcgggttt acagacagta
    41341 ccatctaaaa agacaattcc cttttgaaag gcttggatat ggtcaggtgt tagaagtcca
    41401 ttaacctcaa cttgataagt cttatcgaca tgatattggg gatggaggag ttggaagccc
    41461 aagggaccgt tatcggtcaa gaggaggagt cccgttgtat ctcggtccag tcggccaacg
    41521 gcatagagct tgtcagactg gatgtcagat ggaagcaggt ccatgacggt cggaagttcc
    41581 ttgtctttgt tggctgtaac gacaccagca ggtttatgaa gcataagata ggtgtgttca
    41641 tagccttgaa tgattcgatc ctgaaaaagg agttcttgta gtcctgtatc gatattttga
    41701 gctagggagc gggctgggca accatcgact agaattttcc ctctgaggag tgcttgcttc
    41761 atggcctttc ggctgatttt ttcttgggct aataaattat ctaaacgcat actgtgccta
    41821 tcttatcata agtcgcttgc tttgaaaagg cttgacgtga aaagaaaagc ggagtgaaaa
    41881 catttacact tgcttgagtt atgttattta tttgaaatta tggtataatc gttcagttag
    41941 aaaataaatt ttgaatatta tagaggaaat catgacaaaa ttaagagaag atatccgtaa
    42001 cattgcgatt atcgcccacg ttgaccacgg taaaacaacc ctggttgacg aattattgaa
    42061 acaatcagaa acgcttgatg cacgtactga attggcagag cgtgctatgg actcaaacga
    42121 tatcgaaaaa gagcgtggaa tcaccatcct tgctaaaaat actgccgttg cttacaacgg
    42181 aactcgtatc aacattatgg acacaccagg acacgcggac ttcggtggag aagttgagcg
    42241 tatcatgaaa atggttgacg gtgttgtctt ggtcgtagat gcctatgaag gaaccatgcc
    42301 acaaactcgt ttcgtattga aaaaagcctt ggaacaagac cttgtcccaa tcgtggttgt
    42361 taacaaaatc gataagccat cagctcgtcc agcagaagta gtggatgaag tcttggaact
    42421 tttcatcgag cttggtgcag atgacgacca gcttgatttc ccagtggttt atgcttcagc
    42481 gatcaacgga acttcttcat tgtcagatga tccagctgac caagaagcga ctatggcacc
    42541 aatctttgac acgattatcg accatatccc agctccagta gataactcag atgagccttt
    42601 gcagttccaa gtgtcacttt tggactacaa tgacttcgtt ggacgtatcg gtatcggtcg
    42661 tgtcttccgt ggtacagtta aggttgggga ccaagttacc ctttctaaac ttgacggtac
    42721 aactaaaaac ttccgtgtta caaaactctt cggtttcttt ggtttggaac gtcgtgaaat
    42781 ccaagaagcc aaagcgggtg acttgattgc cgtttcaggt atggaagaca tctttgtcgg
    42841 tgaaaccatc actccgacag atgcagtaga agctcttcca atcctacaca tcgatgagcc
    42901 aactcttcaa atgactttct tggtcaacaa ctcaccattt gctggtaaag aaggtaaatg
    42961 ggtaacttct cgtaaggtgg aagaacgctt gcaggcggaa ttgcaaacag acgtttccct
    43021 tcgtgttgac ccaactgatt caccagataa atggactgtt tcaggacgtg gagaattgca
    43081 cttgtcaatc cttatcgaaa caatgcgtcg tgagggctat gaacttcaag tatctcgtcc
    43141 agaagttatc gtaaaagaaa tcgacggtgt taaatgtgaa ccatttgaac gtgtacaaat
    43201 cgacactcca gaagaatacc aagggtctgt tatccaaagc ctttctgaac gtaaaggtga
    43261 aatgttggat atgatttcaa ctggtaatgg tcaaactcgt ttggtcttcc ttgttccagc
    43321 gcgtggtttg attggatact caactgagtt cttgtcaatg actcgtggtt acggtatcat
    43381 gaaccatacc ttcgaccaat acttgccatt gattccaggg gaaattggtg gacgtcaccg
    43441 tggtgccctt gtttctatcg atgctggtaa ggctacaact tactcaatca tgtctatcga
    43501 agaacgtggt acgatctttg tcaacccagg tactgaggtt tatgaaggaa tgatcatcgg
    43561 tgaaaactct cgtgaaaatg acttgacagt taacatcacg aaggcaaaac aaatgaccaa
    43621 cgttcgttca gctactaagg accaaacagc tgttatcaag acgcctcgta tcttgacact
    43681 tgaagagtct cttgagttct tgaacgacga tgagtacatg gaagtaacgc ctgagtctat
    43741 ccgtttgcgt aaacaaatcc ttaacaaagc agagcgtgag aaagctaaca agaagaaaaa
    43801 atcagctgaa taagagctag aaagagagaa agatggtcta tttagtccta ggaattttac
    43861 tgctcctact ctatgtattt gcgacaccag aaagcattaa agggacagtc aatatcgtcg
    43921 ctatggtatg tattttagtg gcactcttga ttttattggt tctatctttt ctgaaaattt
    43981 ttcaattacc aacagaaata ttcctagcaa tagccatgtt gctcctagct tactttagtg
    44041 ttagagacat tacactcatg ccagtcaaaa aaagtaaaag aagataaaaa agagagtttt
    44101 ggctctcttt tttgctagga attttacaag attgcaattt atttttgagt tatcaccatc
    44161 tcttttgttt ggataatatg aatgggaaaa acttgaataa aattcgttaa tggaatctat
    44221 tttatatttg gttagtactg gttgcat
//
LOCUS       DAVKFH010000012        41619 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKFH010000012 DAVKFH010000000
VERSION     DAVKFH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.012"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..3298
                     /locus_tag="VW006_000679"
     CDS             <1..3298
                     /locus_tag="VW006_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9379572.1"
                     /translation="GAKGEKGDQGEQGLQGRDGEQGPKGEDGKNPTVKVTDGQDGTHT
                     ITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKL
                     KEVVIADGKQGPKGDKGADGAKGEKGAQGERGLTGAQGAKGADGAAGRDGRDGRDGKD
                     VLNGKVNPEANQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTP
                     EVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGQNGNPPHT
                     IVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDP
                     ANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQ
                     DGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAP
                     KIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNG
                     IDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSH
                     TITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKD
                     GKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTI
                     VITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTETIIKDGKSPVATV
                     RDNQDGTYTIRVENGNGTVSETTVRDGKSPTAKVVDNGDGTHTITVVNSDGTTTTTTV
                     RDGREPKLEVIDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVD
                     SDGREYKSIIKDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKS
                     PKVSVEDNGDGSHTITIINSDGTVTKTVIKDGKDGRDGRDGRDGKDGKDGKDGKCGCQ
                     DKPVTPSNDKPVPPTPNVPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKP
                     VPAVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILL
                     SLYVGRRKED"
     gene            complement(3535..4083)
                     /locus_tag="VW006_000680"
     CDS             complement(3535..4083)
                     /locus_tag="VW006_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9379573.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            4404..4892
                     /locus_tag="VW006_000681"
     CDS             4404..4892
                     /locus_tag="VW006_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9379574.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            4889..6076
                     /locus_tag="VW006_000682"
     CDS             4889..6076
                     /locus_tag="VW006_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9379575.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            6069..6434
                     /locus_tag="VW006_000683"
     CDS             6069..6434
                     /locus_tag="VW006_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9379576.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            6450..7796
                     /gene="asnS"
                     /locus_tag="VW006_000684"
     CDS             6450..7796
                     /gene="asnS"
                     /locus_tag="VW006_000684"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9379577.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            7949..8239
                     /locus_tag="VW006_000685"
     CDS             7949..8239
                     /locus_tag="VW006_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9379578.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            8251..8721
                     /gene="ssbA"
                     /locus_tag="VW006_000686"
     CDS             8251..8721
                     /gene="ssbA"
                     /locus_tag="VW006_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9379579.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            8753..8992
                     /locus_tag="VW006_000687"
     CDS             8753..8992
                     /locus_tag="VW006_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9379580.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            9287..10687
                     /locus_tag="VW006_000688"
     CDS             9287..10687
                     /locus_tag="VW006_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9379581.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            10689..11048
                     /locus_tag="VW006_000689"
     CDS             10689..11048
                     /locus_tag="VW006_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9379582.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            11102..11851
                     /locus_tag="VW006_000690"
     CDS             11102..11851
                     /locus_tag="VW006_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9379583.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            11841..12140
                     /locus_tag="VW006_000691"
     CDS             11841..12140
                     /locus_tag="VW006_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9379584.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            12211..13146
                     /locus_tag="VW006_000692"
     CDS             12211..13146
                     /locus_tag="VW006_000692"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9379585.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            13232..13744
                     /locus_tag="VW006_000693"
     CDS             13232..13744
                     /locus_tag="VW006_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9379586.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            13769..14377
                     /locus_tag="VW006_000694"
     CDS             13769..14377
                     /locus_tag="VW006_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9379587.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            14478..14675
                     /locus_tag="VW006_000695"
     CDS             14478..14675
                     /locus_tag="VW006_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9379588.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(14718..16163)
                     /locus_tag="VW006_000696"
     CDS             complement(14718..16163)
                     /locus_tag="VW006_000696"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9379589.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVKDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            16269..17891
                     /locus_tag="VW006_000697"
     CDS             16269..17891
                     /locus_tag="VW006_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9379590.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLCSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            18095..20053
                     /locus_tag="VW006_000698"
     CDS             18095..20053
                     /locus_tag="VW006_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9379591.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSVQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            20225..20458
                     /locus_tag="VW006_000699"
     CDS             20225..20458
                     /locus_tag="VW006_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU9379592.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            20464..21906
                     /locus_tag="VW006_000700"
     CDS             20464..21906
                     /locus_tag="VW006_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379593.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIYSSSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            22064..23158
                     /locus_tag="VW006_000701"
     CDS             22064..23158
                     /locus_tag="VW006_000701"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9379594.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            23166..24332
                     /locus_tag="VW006_000702"
     CDS             23166..24332
                     /locus_tag="VW006_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9379595.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            24425..27523
                     /locus_tag="VW006_000703"
     CDS             24425..27523
                     /locus_tag="VW006_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9379596.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            27571..28188
                     /locus_tag="VW006_000704"
     CDS             27571..28188
                     /locus_tag="VW006_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9379597.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            28199..29533
                     /locus_tag="VW006_000705"
     CDS             28199..29533
                     /locus_tag="VW006_000705"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9379598.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            29543..30007
                     /locus_tag="VW006_000706"
     CDS             29543..30007
                     /locus_tag="VW006_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379599.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            30004..30498
                     /locus_tag="VW006_000707"
     CDS             30004..30498
                     /locus_tag="VW006_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379600.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            30579..32234
                     /gene="mltG"
                     /locus_tag="VW006_000708"
     CDS             30579..32234
                     /gene="mltG"
                     /locus_tag="VW006_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9379601.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            32305..32787
                     /gene="greA"
                     /locus_tag="VW006_000709"
     CDS             32305..32787
                     /gene="greA"
                     /locus_tag="VW006_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9379602.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            33295..33693
                     /locus_tag="VW006_000710"
     CDS             33295..33693
                     /locus_tag="VW006_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379603.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            34138..35145
                     /locus_tag="VW006_000711"
     CDS             34138..35145
                     /locus_tag="VW006_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9379604.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            35220..35420
                     /locus_tag="VW006_000712"
     CDS             35220..35420
                     /locus_tag="VW006_000712"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9379605.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            35455..36171
                     /gene="atpB"
                     /locus_tag="VW006_000713"
     CDS             35455..36171
                     /gene="atpB"
                     /locus_tag="VW006_000713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9379606.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            36185..36679
                     /gene="atpF"
                     /locus_tag="VW006_000714"
     CDS             36185..36679
                     /gene="atpF"
                     /locus_tag="VW006_000714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9379607.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            36679..37215
                     /locus_tag="VW006_000715"
     CDS             36679..37215
                     /locus_tag="VW006_000715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9379608.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            37230..38735
                     /locus_tag="VW006_000716"
     CDS             37230..38735
                     /locus_tag="VW006_000716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9379609.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            38751..39629
                     /locus_tag="VW006_000717"
     CDS             38751..39629
                     /locus_tag="VW006_000717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9379610.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            39715..41121
                     /gene="atpD"
                     /locus_tag="VW006_000718"
     CDS             39715..41121
                     /gene="atpD"
                     /locus_tag="VW006_000718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9379611.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            41132..41551
                     /locus_tag="VW006_000719"
     CDS             41132..41551
                     /locus_tag="VW006_000719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9379612.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
BASE COUNT        12810 a         7739 c         9287 g        11783 t
ORIGIN      
        1 aggtgctaaa ggtgaaaaag gggaccaagg tgaacaaggt cttcaagggc gtgatggtga
       61 acaaggacca aaaggagaag atgggaaaaa cccaacagtt aaagtaactg atggtcaaga
      121 tggaacgcat acaattacaa ttaacgatgg taaaggtggt ataactacta cagtagtaag
      181 agatggattt gatggtgcaa gccctcttgt ttctactcat agaaatgaag cagataaaac
      241 aacaactgtt attttctatt atgatctaaa tgataataat caatttgatg aaggagatac
      301 aaaacttaaa gaagttgtta tcgcagatgg aaaacaagga ccaaaaggtg acaaaggtgc
      361 agatggtgcc aagggtgaaa aaggagccca aggagaacgc ggcctgactg gagcgcaagg
      421 agctaaaggt gcggatggag cagcaggtcg tgacggacgt gatggacgtg acggtaaaga
      481 cgtgttgaac ggcaaagtta acccagaagc aaatcaaggt aaagacggtg ataaatacgt
      541 caatacagaa acaggcgacg tcttcgttaa gaataacggc aactgggata aagagggcaa
      601 catcaaggga cctaaagggg acaaaggtga acgcggagaa gatggtaaga ctccagaagt
      661 aactgtaact ccaggtaaag atggccatag tactgacatt acattcactg ttccaggtaa
      721 agatccagtt acagtaaatg ttaaggacgg agaaaatggt ctgaacggta aaactccaaa
      781 agttgattta cttcgtgtcc aaggacaaaa cggaaatcca cctcatacaa ttgtgacatt
      841 ctatacagat gaaaacaatg atggcaaata tacaccagga actgatgaac ttctaggttc
      901 agaaatgatt aaagatggtg ctaaaggtgc ggacggacga gatggtaaat cattgcttac
      961 tgtcaaggat ggtaaagaaa ctaaagttta ccaagaagat ccagctaacc caggacaacc
     1021 attaaatcca gaaaaaccac ttgcggtaat tagagatgga gtagatggaa aatcacctac
     1081 agttacagct gttcgtaaag atgaagcagg gcataaaggt gtagaaatca ctgttgataa
     1141 ccatgatggt tcacaaccaa ctacagtctt tgttcaagat ggtgctaagg gagaaactgg
     1201 tgcaaccggt caagatggac aaactcctac aatcactact caacgtggac aagatggcca
     1261 aagcactgtt gtaactatca caacatcagg taaagatcca gtaaccttca ctgtaaaaga
     1321 tggtaagaat ggtaaagatg gccgtgcacc gaaaatcaaa gtagaagata ttacttcacc
     1381 ttcaagaatt agacgcgata cagatgctgc tgcaactcca acgcgtaacg gtatccgtgt
     1441 tacagtttat gatgatgtta atgacaatgg ggtatacgac gaaggtgtcg ataaagtatt
     1501 aaatagtaaa gatatttata acggtataga tggacgtgat ggttcagctc caactattac
     1561 tacaaaagat aatggagatg gaactcacac tatcacagtt caaaacccag atggttctga
     1621 atcaacaaca gttgttaaag atggtaaaga cggtaaaact gcgaatatca ctacaacaga
     1681 aaacccagat ggaagccaca caattacggt gacaaatcca gatggttcaa ctaaagaaac
     1741 tgttgttaaa aacggtaaag acggtaagac tcctaaagtt gaagtaacgg ataacaacga
     1801 tggaactcat actgttaaag ttacagatgg agacggcaat gttaccaacg ctatcatcaa
     1861 agatggtaaa gacggtaaag ctgcaacagc aacaactact gaaaatccag atggaagcca
     1921 cacagtaaca atcactaacc cagacggaac taagaatgag tttgttgtta agaatggacg
     1981 tgacggtgtt gacggacgta ctccaaccgc atctgttcgt gataatggag acggaagtca
     2041 tacaatcgtt attacaaatc cagaaggtgt gacaactgaa accacagttc gtgatggtaa
     2101 atcaccaaaa gtgactataa ctgatgaaca aaatggaact cataagatct ctgttctaaa
     2161 tggtgacgga acaactactg aaacaatcat taaagatggt aaatcaccag tagcaacagt
     2221 tagagataac caagatggta cttacactat tcgtgtggaa aacggtaatg gtactgtttc
     2281 tgaaaccaca gttcgtgacg gtaaatcacc aactgctaag gttgtggata atggagatgg
     2341 aactcacact atcacagttg tgaactcaga cggaacaact acaacaacta cagttcgtga
     2401 tggtagagaa ccaaaacttg aagttattga taacaacgat ggttcacaca ctattaaagt
     2461 gacaggtgct gatggtaaag gaacgacaac tacaatcttt gatggtaaat caccaaaagc
     2521 gaacatcgtt gataacggag atggaactca tacattaaca atcgtagatt ctgatggtcg
     2581 tgaatacaaa tctattatca aagatggtaa agacggcaaa gatagcgttt caccaactgt
     2641 aactgttaaa aataataacg atggaactca cgttgttaca atcactaatc cagatggaag
     2701 taagacagaa atggtgatta aagacggtaa agatggtaaa tcaccaaaag tttctgttga
     2761 agataatggt gatggtagtc atacaatcac aatcatcaat tctgatggaa ctgtgacaaa
     2821 aacagttatt aaagatggca aagatggtag agatggacgt gatggtcgag acggcaaaga
     2881 cggtaaagac ggtaaagatg gaaaatgtgg atgccaagac aaaccagtaa caccatcaaa
     2941 tgacaaacca gttcctccaa caccaaatgt gccgacacca gaagtaccgg ttaaacctgt
     3001 gccagcgcaa ccaacaccaa atgtaccgac accagaagtg ccagtacaac caactccagc
     3061 tgttccaaca ccagaagtac cggttaaacc agtaccagcg gttccagaac aaccagtagt
     3121 accaacaccg gctcaaccag caactccagt aaatgctaat ccagtagcac caactacagg
     3181 taaagaaaac cgtggggaca aattacctga aactggaagc caatctgatt atatctctgt
     3241 tcttttaggt agcggtattc tattgagcct atatgtagga cgaagaaaag aagattaatc
     3301 agccaaaagc agtcactaat ttctcgaaag aagatgataa gtgatagatc tcgaatgaaa
     3361 gaatattctt agaatgattt tcctatgatg gaaaagactg ctaaaaacct tgagtggtaa
     3421 cgctcaaggt ttttttggat ggctgatgtt tgtctgaaaa tcttgattga gaaataaaaa
     3481 gccaccctat taggtggcta taattgattt agactttcta aaattttcta ttttttactg
     3541 aatcgcttcg accaagtctt gagcttgttt ttcaagtgag tttaggactg tttcttcaag
     3601 aaccaatttt ccgtctgccc aggcagagtc attaacacgt gcagcagtga aatcaccaac
     3661 gccttgtgta cggataaatg gcaagaggtc tttgtagata gcgaaaagtt gatcgtgccc
     3721 tgcattggct acagatgata ctgtgacaaa cttgtcttga agggcagaaa cgccacgtgt
     3781 atcagacaag tcaagggcac gagatagcca gtcaagcaag tttttcactg taccagggat
     3841 agagaagttg tagactggag agaaaatcca gatagcatcc gcaacgagaa ctgcttcacg
     3901 agcagcagct acagctggat gtgttggaac ttccaaatct tggctgaaga gaggaagggc
     3961 tgaataatca aggtagctaa cttccgcttt accagcaagt gctttctcag cttcgagcgc
     4021 catttggtgg ttgaaagaac cttgacgtag tgatccgacg ataaataata cttttttaga
     4081 catgatgtgt ctcctttttc ttaaaatttc gagaactctc tcgttgttat aaaatatatt
     4141 acaacaaaac aaaagaactt gcaagaaatt tgctcagagt ttatttgaaa attgtaaaat
     4201 ttcggaaact ctaatcattt gtgactgaaa aagtaagaac tgtttcactt tcaaaatcgg
     4261 ttttgtttga agggagaaat agaattggtt gctcaaagca cagctttgag gttgcagata
     4321 aagctgacgt ggtttgaaga gattttcgaa gagtattctt atattcactt gtaataaagc
     4381 ttaatagatg gtaaaataga cgagtgaaaa aaagacaaaa caaagcaaaa aataatctac
     4441 tgtggcagta tggtctaggg atgacgattt tgtttgtggt tatcagtggt tcctttctgt
     4501 atatggtttc tcttagcatg aaaccctatc aaacagctaa aagtgaagga gaaaaattag
     4561 ctcagcagta tgcaggatta gagcaggtcg atcaggttga tttatacaat ggcttggaat
     4621 cttattacag cgttcttggt cgtaataaac agcaagaagc gcttgctgtc ctgattggta
     4681 aagatgacca taagatttac gtttatcagc taaatcaggg tgtttcacaa gaaaaagcag
     4741 aaacggtttc taaggaaaag ggagctggcg agattgacaa gataaccttt ggccgttatc
     4801 aagataagcc aatctgggaa gttaagtcag gatctgattt ttatctagta gattttgaaa
     4861 caggagcatt ggtcaacaag gagggcctat gaaactatcc aaccgtgttt tagaaatgga
     4921 agaaagtgtg actttggctg ctggagcccg tgccaaagca ctgaaggctg agggtagaga
     4981 tattttgtct ctaaccttgg gtgagccaga tttcactact cccaaaaata tccaagatgc
     5041 cgccattgca tcgattcgag atgggcgtgc ttctttttat acagtaacct caggtctgcc
     5101 agagcttaag gcggcggtca atagctactt tgagcgcttt tacggctatt ctgtagcgtc
     5161 aaatcaagtg acagtcgctg cgggagccaa atattctctc tataccttct ttatggctgt
     5221 ggtcaatcca ggtgatgaag tcatcatccc aaccccatac tgggttagct atggagatca
     5281 ggtcaagatg gcagaaggcg ttcccgtctt tgtttctact aaggaagaca atcactttaa
     5341 ggtgaccgta gatcagttag aagcagctcg cactgacaag accaaggttt tggtgctgaa
     5401 ttcgccatct aatcccacag gtatgattta cacccgtgag gaactcttgg caattggaaa
     5461 ctgggctgta gaaaatgata ttctcatcct agcagatgat atctatggcc gcttggttta
     5521 taatggtcat gagttcacac cgatttctag cctatcggaa gcgattcgca agcaaacagt
     5581 ggtcatcaat ggtgtgtcta aaacctatgc catgactggt tggcggattg gttatgccgt
     5641 tggagaagca gatattatcg ctgccatgtc caagattgca ggtcaaacaa cttcgaatcc
     5701 gtcagcagta gcccaatatg cagcagttga ggctctatca ggtgagcaag atactgtaga
     5761 aagcatgcgt caggcctttg aggaacgtct taataccatc tatcccctcc ttgcagaggt
     5821 accaggattt gaagtggtca aaccgcaagg ggccttctat ctcttcccaa atgtcaaaaa
     5881 ggccatggag atgaaaggct acacggatgt gacagacttt acaactgcta tcttagaaga
     5941 agccgaagtg gccttggtaa caggagctgg atttggagca ccagaaaatg tccgcctcag
     6001 ctatgcgaca gacctagaca cgcttaaaga agcagtcgaa cgcttgaaag catttatggg
     6061 tagtgagaat gattgatcat tttgagatta aggtaaagga tttacaaatt tcagaaggat
     6121 tttataggag ttttctcgct cctttggact ataaattggc ttttaagact agttctctaa
     6181 ttagttttct atccccgaac agccctcatc ctggtggtga tttttggctg actcagggaa
     6241 cacaagatcc tgttcatttt gcttttttag cagaaaataa ggaagaagtt caggcttgtt
     6301 atgaggctgg gttagaggca ggtgggcgag acaatggggc tcctggttat cgaagtgagc
     6361 atccgattta ctatgctgct tttatgattg acctggatgg gaacaatata gaggtggttt
     6421 gccataaaga ataaaaaaga aaaggaaaaa taatgacaaa acgtgtaacg attattgacg
     6481 taaaagacta tgttggtcag gaagtgacga ttggcgcttg ggttgccaac aaatcaggaa
     6541 aaggaaaaat cgctttctta caattgcgtg atggaacagc cttctttcaa ggtgtggctt
     6601 ttaaaccaaa ctttgtcgaa aaatttggtg aagaagtggg acttgagaag tttgatgtta
     6661 tcaaacgctt gagccaagaa acgtctgttt atgtgacagg tattgtcaaa gaggacgaac
     6721 gttctaaatt tggctatgag ttggacatca cagacatcga agtgatcggt gaatctcaag
     6781 actacccaat cacaccaaaa gaacacggaa cagacttttt gatggataac cgtcacttgt
     6841 ggctacgctc tcgtaagcaa gtagctgtgt tgcaaatccg taacgctatt atctatgcaa
     6901 cttatgagtt ctttgacaag aacggtttta tgaagtttga cagcccaatt ctttcaggaa
     6961 atgcggcaga agattctaca gaactctttg aaactgacta cttcggaacg ccagcctact
     7021 tgagccaatc aggtcagctt tacctagaag caggggctat ggctcttggt cgtgtctttg
     7081 actttggtcc agttttccgt gctgaaaaat caaaaacacg ccgtcacttg actgagttct
     7141 ggatgatgga tgctgagtac tcatacttga cacatgatga gtcgcttgac ttgcaagaag
     7201 cttatgtgaa agctcttcta caaggtgttc ttgaccgcgc gcctcaagcc ttggaaacct
     7261 tggaacgtga tacagaactc ttgaaacgct acattgcaga gccattcaaa cgtatcactt
     7321 acgatcaagc cattgacctc ttgcaagagc atgaaaatga tgaagatgct gactacgagc
     7381 atcttgagca tggtgatgac tttgggtcac cacacgaaac ttggatttca aaccactttg
     7441 gtgtgccaac atttgtcatg aactatccag cagccatcaa ggccttctac atgaaaccag
     7501 ttcctggaaa tccagagcgc gtgctttgtg cagacttgct tgctccagaa ggctatggag
     7561 aaattatcgg tgggtctatg cgtgaggaag attacgatgc ccttgtcgct aagatggatg
     7621 aacttggcat ggatcgtaca gaatatgaat tctaccttga ccttcgtaaa tacggtacag
     7681 ttccacacgg aggatttggt atcggtatcg aacgtatggt aaccttcgca gcaggaacaa
     7741 aacatatccg tgaagctatt ccattcccac gtatgttgca ccgtatcaaa ccataagaac
     7801 aggaaaacgc ccatgtggcg ttttttcgaa ttctagtgtt tttacttgcg taaaaaaatt
     7861 ttttctagta taatagttta ttgtgagcga acctcactta ccccttgcaa agtcttgggg
     7921 tcattagacc aaaaggagga acatatcaat ggctaaatac gaaattcttt atatcattcg
     7981 tccaaacatt gaagaagaag ctaaaaacgc tttggtagca cgttttgact ctattttgac
     8041 tgacaacggt gcaactgttg ttgaatcaaa aacttgggaa aaacgtcgtc ttgcatacga
     8101 aatccaagat ttccgtgaag gactttacca catcgttaac gttgaagcaa atgacgatgc
     8161 agctcttaaa gagtttgacc gtctttcaaa aatcaacgct gacattcttc gtcacatgat
     8221 cgtcaaaatt gacgcgtaag aaggtaaact atgattaaca atgttgtact tgtagggcgt
     8281 atgacacgtg acgctgagtt gcgttatacc ccatcaaatg tagcagttgc gacttttact
     8341 cttgcagtaa accgtacatt taagagtcaa aatggtgaac gtgaggctga ttttatcaat
     8401 gtcgttatgt ggcgccaaca ggctgaaaat cttgctaact gggctaaaaa aggctcactt
     8461 atcggggtga caggtcgtat ccagactcgt agttacgata accagcaagg acaacgtgtc
     8521 tacgtgacag aggtcgtggc tgagaatttc caaatgttgg aaagccgtag tgtgcgtgag
     8581 ggccacacag gtggagctta ctctgcacca actgcaaact attcagcacc tacaaattca
     8641 gtaccagact tttcacgtaa tgaaaatcca tttggagcaa caaacccatt ggatatttca
     8701 gatgatgatt taccattcta atggacaact aaacttataa aggagaaaaa acatggctca
     8761 acaacgtcgt ggcggattca aacgccgtaa aaaagttgat tacatcgcag caaacaaaat
     8821 tgaatatgtt gattacaaag atactgagct tcttagccgt ttcgtttcag aacgtgggaa
     8881 aatccttcct cgtcgtgtaa caggaacttc agctaaaaac caacgtaaag taacaacagc
     8941 tatcaaacgc gctcgcgtaa tggctttgat gcctttcgta aacgaagatt aaagaaaaga
     9001 cccggttggg tctctttttc atgagcttga aaacaagaaa gcgaattaag accccaacgg
     9061 gtcttttttc acaagctttg aaatgagaga gcgagttagg ctcagtggct ccctttttac
     9121 gagtttgaaa acaagagaac acattaaaag cacgatggag ctttttcata aatatgtctt
     9181 ttttctcatc caagaaatta aaataaaaat gataaacttt ttgaaaatag gggatttgcc
     9241 tactttatga tataatggaa agagttagat tgaaagaggt agtgagatgg acgcgaaatt
     9301 aagatacaag gcaaagaaga tcaagattgt cttttttgat attgatgata cattgcggaa
     9361 ttcaaagaca gggtttattc caactacaat tcccactgtt tttaaacagt tgcgtgaaaa
     9421 aggaatttta acaggaatcg cctctggacg tggcattttt ggtgttgttc cagagattcg
     9481 tgatctcaag cctgactttt ttgtaacttt gaatggagct tatatcgaag ataaaaaagg
     9541 tcaggtcatt tatcagcatc agattgaaaa gtcagatgtt gaggagtata tctcttgggc
     9601 taagcaagaa ggaattgagt atggcttggt tgggagtcat gatgccaagt tgtcgactcg
     9661 caccgatatg atgagtgaag ctatcaatcc aatttatccc gacttagatg tagatcccga
     9721 tttccatgaa aaagaagata tctatcagat gtggactttt gaagataagg gagatgactt
     9781 gcacttgcct gacagtctct cagacaaact tcgcatggtt cgttggcatc aacattcgtc
     9841 tgatattgtg ccgatttcag gctccaaagc gacgggggtg gaaaaggttg tggaacacct
     9901 tggcttgaaa ccagagaagg tcatggtttt tggagatggt ctcaacgact tggaactctt
     9961 tgattatgct ggaatcagcg ttgccatggg aatttctcat gataaaatca aagaaaaagc
    10021 agattatatt acaaaaacat tagaagaaga tggcattttt gatgccttag aagtatttgg
    10081 tatggtagaa aaagaattgc attttccaca agtagacatt gaaacagtag aaggtcctct
    10141 tgcgactatt aagaccaatc acggagactt acgtatcaag cttttccctg aacatgctcc
    10201 taaaacagtg gctaactttg tatctctttc aaaagatggc tactatgatg gtgtcatttt
    10261 ccaccgtatt atcaaggact ttatgatcca aggtggagac ccaactggaa ctggtatggg
    10321 tggcgagtca atctacggcg aatcttttga ggatgaattc tcagaagagc tttacaatat
    10381 ccgtggtgct ctttccatgg caaatgctgg tccaaatacc aacggcagcc agttctttat
    10441 cgtgcaaaac caacacctac cttattcgaa gaaagaaatt actcgtggtg gttggccaga
    10501 accgattgca gaaatctatg ccaatcaagg tgggacacct cacctagacc gccgtcacac
    10561 ggtttttggt cagttagctg atgaagcatc ttacgctgtc ttggatgcca ttgctgctgt
    10621 tgagacagga gctatggaca agccagttga agatgttgta attgaaacta ttgaaatcga
    10681 ggactaagat gaaaatcggt gataagctaa aggggcgtat tacagggatt cagccctacg
    10741 gtgcctttgt tgagttagag acgggtgata cggggctgat tcacatttca gaaattcgga
    10801 caggatttat tgaaaatatt catgaaacct tgaaagtcga tgaagaggtt caagttcagg
    10861 tagtggattt agatgaattt acggggaaag ccagtctttc tatccgcact ttggaggaag
    10921 aaaagtacca gtttcctaga cggagacgct tttcaagcga ccgctttaac tacggttttg
    10981 ctccctttcg acgaatgcta ccaatctgga ctggagaagc cctgcaccat ttaaaaaaga
    11041 agaagtagtt tagagtttta tattttttgt aatgttaata tgaatttgct ataataggac
    11101 catggaagaa gaacaattat taaaatcagg agagcgcatt aaccagctct tttcgacaga
    11161 tatcaaaatc attcaaaata gagaggtttt tagctattcg gtggatagtg ttctcttatc
    11221 acgatttcca cgttttccta agaaggggtt gattgtggat ttctgtgctg ggaatggagc
    11281 agtggggctt tttgctagca ctcgtactca agcacagata ttgtctgttg agattcagga
    11341 gcgtttggcg gatatggctg aacgctctgt ccgtttgaat ggattagagg agcaaatgca
    11401 ggtcatctgc gatgatttga aaaatatgcc tgctcacatt caggggagta aggtggatat
    11461 gattttgtgt aatccaccct atttcaaggt gaatccttat tctaatctga acgagagtga
    11521 acattatctc ttggctcgac acgaaatcac gactaatttg gaagaaatct gtcgtagtgc
    11581 ccagagtatt ctcaagtcta atgggcgttt ggccatggtt catcgtcctg atcgacttct
    11641 ggatattttg gatacgttaa aacgacataa tctagcccct aagcgcctgc agtttgttta
    11701 tccaaaaaga gaaaaggaag ccaatatgct tttgattgag gcgatcaagg atggctcaac
    11761 aagtggcttt aaggtcttac ctcctttgat tgtccacaat gatgatggct cttatacgcc
    11821 cgaaatcgaa gagatttatt atggatcata aggcttatat gtacgttctg gagtgtcgtg
    11881 atggatccta ctatataggc tatacgactg atatgagaag acgcctcgct atccacaata
    11941 gtgggaaggg agccaaatat acacgagcac gcttgccagt caaacttatc tatgctcaag
    12001 gttttgccag taaggaagaa gccatgtcgg ctgaagctct tctcaagcgt aagaagaggc
    12061 cacagaagga agaattttta tctgaaaatc aagatagaaa tttactcagt tattttgaag
    12121 aatcgtgggg agtcctttga ctcctcttac ctttgtcaaa aagcgaaaaa aatgctacaa
    12181 taaagaaaat aaacaaaatg aggtattatc atgtccaaga ttctagtatt tggtcaccaa
    12241 aatccagact cagatgccat cggatcatct gtagcttttg cctaccttgc aaaagaagct
    12301 tacggtttgg atacggaagc tgttgccctt ggaactccaa atgaagaaac agcctttgtc
    12361 ttgaactatt ttggtgtgga agcaccacgt gttatcactt ctgccaaagc agagggggca
    12421 gagcaagtta tcttgactga ccacaatgaa ttccaacaat ctgtatcaga tatcgctgaa
    12481 gtagaagttt acggtgttgt agaccaccac cgtgtggcta actttgaaac tgcaagccca
    12541 ctttacatgc gtttggagcc agttggatca gcgtcttcaa tcgtttaccg tatgttcaaa
    12601 gaacatggtg tagctgtgcc taaagagatt gcaggtttga tgctttcagg tttgatttca
    12661 gatacccttc ttttgaaatc accaacaaca cacccaacag ataaaatcat tgctcctgaa
    12721 ttggctgaat tggctggtgt gaacttggaa gaatatggtt tggcaatgtt gaaagctggt
    12781 accaacttgg ctagcaaatc tgctgaagaa ttgattgata tcgatgctaa gacttttgaa
    12841 ctcaacggaa ataatgtccg tgttgcccaa gtgaacacag ttgacatcgc tgaagttttg
    12901 gaacgccaag cagaaattga agctgcaatg caagctgcca acgaatcaaa cggctactct
    12961 gactttgtct tgatgattac agatatcgtc aactcaaact cagaaatctt ggctcttggt
    13021 gccaatatgg acaaggtcga agcggctttc aacttcaaac ttgaaaacaa tcatgccttc
    13081 cttgctggtg ccgtttcacg taagaaacaa gtggtacctc aattgactga aagctttaat
    13141 gcgtaagatt ttgggtgtca gctcaaaatc ggaaagtcta gtttgcctta tatcgaaagg
    13201 agtttcggct ccttttttct aggagtgaag tatgttagaa aatggcgatt tgatttttgt
    13261 gagagatggg tcagacatgg gacaggccat ccagacttcc acaggtaact atagccatgt
    13321 tgccatttat ttggatggga tgatttatca tgctagtgga caggctggtg ttgtctgtca
    13381 agaaccggca gacttctttg agtccaatca tttatacgac ctctatgttt acccagaaat
    13441 ggatatccag tcggtgaagg aaagagcttg caaacatctt ggagcaccct acaatgcttc
    13501 tttctatcca gatgcacctg gtttctactg ctcccagtat atagcagaaa tcctacctat
    13561 ttttgaaact attcctatga aatttggaga tggggagcag gagattagtg atttttggag
    13621 ggagtattac atagaactag gtctgcctgt tcctctgaac caagctggta ccaatcctag
    13681 tcagttggca gcatcgcctc tgttacaatg taaagaaagg aatcttcatg attcagattt
    13741 ttaatccatc tcgtttgacg agacagccat ttttggagaa ttgatccgct atctggacca
    13801 gtatgaggat gtgattctac gggaaattaa ggctcaattt ccagatgttg cagttgataa
    13861 actcatggaa gagtatataa aggcaggctt gattctacgt gaaaataagc gctattacct
    13921 caattttcct acgcttgaat cacttgatag tcttgaactg gatcaagaga tttttgtcag
    13981 agaagctagt ccggtctatc aagccttgtt ggagcagagt tttgagacgg aattgcgcaa
    14041 tcaaatcaat gcagctattt tagttgaaaa gacggacttt gcgcgcatta aaatgactct
    14101 gtccaattat ttttacaagg tcaaacagca gtatcctttg acagaaaaac agcaggagct
    14161 ctatgacatt ttaggagatg ttaatcctga gtatgcactc aagtatatga cggctttttt
    14221 gttgaaattt ctcaaaaaag accagcttat gcagaaatgc cgtgatatct ttgtggacag
    14281 tttggttgtc ttaggctata ttgtgcaaaa tgaagatgga aagtatgagt tggctatcga
    14341 ttttgataag gagagattaa ctttctactt agcgtgattt cttgtttctg agtacattgt
    14401 ttgactttcc ttagtattcg gtataaacta tatgtaaccg gtaacacata tcggaataaa
    14461 ctaaaggaga caatcatatg tcacttgaaa acaaattgga acaagcaaca ggcgctgtca
    14521 aagaaggttt tggtaaagtt actggagaca gcaagacaga acttgaagga gctgttgaaa
    14581 aaacagttgc taaggcaaaa gacgttgtag aagacgcaaa aggtgctgta gaaggtgccg
    14641 ttgaaggttt gaaaaacgtt tttactaaag aataggaaaa aatcaagggt ttcattttcc
    14701 cttgattttt tctattctta taaataattt tctgcgacgg ctgtatctcc tgggtaggat
    14761 tctttcttgc cctggatgat ttggtaacaa tcggctccct tacccgcaat aataactgca
    14821 tctaattcgt gatttgtgat agccattgcc gccttgatgg cttcttggcg atccgcaatc
    14881 ttctcaacag gatgattgat gtagctacta atttcatctg caatggccat tgggtcttca
    14941 tagttagggt catcagcagt cagaaagact tgaatctcag ggtgttgatt gaggaggagg
    15001 ccaaagtcct tacgacgact ttctcccttg tttcctgttg atcccagaac cagagcaatc
    15061 tttccggttt gatgagtttc aaccacattg atgagttttt tcagactatc cccattgtgg
    15121 gcatagtcga tgaagacctt ggctccattt ttctgagtga ggacttccat acgaccagga
    15181 acgcgggttg cagcgatgcc ttttttgatg tcctcaagac ttgctccgag acggagacaa
    15241 gcaagtccag cagcaactgc attttcttgg ttgaagttgc caatgagttg gatatcataa
    15301 tctccagcga gtttacccgt agctgaaaag ctaaaggctt tggaattctc gatttggtta
    15361 tcaaattggc taccatagaa atcatggtct tgatctttaa cctgttcttt caagactgag
    15421 aagtggtcca tgtcactgtt aatgatgact gctcggctat tttccatcaa gagacgcttg
    15481 tggtagaaat agtcttcaaa gctagggtgt tcaatcgggc cgatatggtc tgggctgata
    15541 ttgaggaaaa ctcccacatc aaaggttaga ccatagacac gtctgaccag ataggattga
    15601 ctggagactt ccatgatgag gtgggtacgg tcattttgca cagcctgatt catcatgtca
    15661 aagaggtcaa tactctcagg ggttgtcaac gctgacttaa agaaagtctc gccatcaaga
    15721 gttgtgttca tggtcgacaa catagcaggt cgatgccctt gagataagat gttataggcg
    15781 aaataggctg ctgttgtctt acccttagta ccagtaaagg caaggagttt gagtttttcc
    15841 tgtggattac catagaactc catggcaatc aaactcatgg ctttctttat atcgttcaca
    15901 atgatgacag ggataccgac ttcgtagtcc ttttcagcta cataccaagc taagccttgt
    15961 gttatagcag aaagaaggta ttctttttta aaggcagcgc cttttgcaaa aaaaagagtg
    16021 tcttctgtta cttttcggct gtcgtagctg atgctatcaa aaacaacttt gctgtagttg
    16081 tagtggtaat gaccttggtc aataatttcg cgaaaaaggc catctttctt taaaatatct
    16141 aatacagttt caatttttat catactttct attgtaaacc gaaagtcgca aatttacaag
    16201 taacaaggaa aagtttataa tggaagataa ggagtttttc ctagttatca aaattgaatg
    16261 aggaatctat gtcgcacgaa aacaatcacc agcaggccca gatgttacgg gggactgctt
    16321 ggctaacggc tagtaacttt atcagtcgcc tactcggggc tgtttacatt atcccttggt
    16381 acatctggat gggggcttat gcagctaagg caaatggtct ctttaccatg ggttacaata
    16441 tctatgcttg gttcttgttg gtttcaacag cggggattcc agttgcggtg gccaagcaag
    16501 ttgccaagta taataccatg cgagaagaag agcatagctt tgccctgatt cggagcttct
    16561 taggctttat gacaggacta ggcctggttt ttgctttagt cttgtatgtc tttgctcctt
    16621 ggctagcaga cttgtctggc gtgggcaaag acttgatccc aatcatgcaa agcttggctt
    16681 ggggagtctt gattttcccg tctatgagtg ttatccgagg atttttccaa gggatgaata
    16741 acctcaaacc ctatgccatg agccaaattg ctgagcaggt cattcgtgtt atctggatgc
    16801 tcctagcaac ctttatcatt atgaagctcg gttcaggaga ttatctagca gccgttaccc
    16861 aatcaacctt tgctgccttt gtcggtatgg tagccagttt tgcagtcttg atttatttcc
    16921 ttgcccaaga aggttcactc aaaagagtct ttgaaacagg agataagatt aacagtaagc
    16981 gtctcttggt tgataccatt aaggaagcca ttccttttat cctgacaggg tctgccatcc
    17041 agctcttcca gattttggat cagctgacct ttatcaatag tatgagctgg tttaccaact
    17101 acagcaatga ggacttggtt gtcatgtttt cttatttctc agccaatcct aataaaatca
    17161 cgatgatttt gatttctgta ggggtttcga ttgggagtgt tggtttgcca cttttgacgg
    17221 aaaactatgt caagggggac ttgaaagcag cttctcgtct ggttcaggac agtctcaccc
    17281 tactctttat gttcttgcta ccagcaacgg ttggagtggt tatggtagga gaacctctat
    17341 atacagtctt ctatggtaag ccagatagtt tggctctggg cttatttgtc tttgcagttt
    17401 tgcagtctat tattttaggc ttgtacatgg tcttgtctcc aatgcttcag gccatgttcc
    17461 gcaaccgcaa ggccgttctc tattttatct atggttctat tgccaagcta gtcttgcaac
    17521 tacctaccat cgccctcttc cacagttatg gtcctttgat ttcaacaacc attgctctca
    17581 tcattcctaa cgtcttgatg tatcgggata tttgtaaagt aactggtgtc aagcgcaagg
    17641 tgattttgaa gcgaaccatt ttaatcagtt tgctgaccct agtcatgttt ctgttaatag
    17701 gaaccatcca gtggctgtta ggatttttct tccaaccaag tggacgtttg tgtagcttct
    17761 tttatgtagc tcttgtcggt gccatggggg gtggacttta tatggttatg agtctgcgta
    17821 cctatttatt agataaggta ataggaaaag cccaagcaga tcgcctgcga gcaaaattta
    17881 agctttcgta aggtagcatt tataaataaa aggaataagt tgaacaatct tttaggagaa
    17941 cttgaagctt attccttttt tagcgtaaaa atagctaaaa ttatatttca aaaatagaaa
    18001 atgaggcttt tttcgatttt ttggaaaaaa tcattgacta aacaaactaa acgttgtata
    18061 ataaaacaaa tatttaaagc aggaggttcc ggaaatgaaa aagtctaaga gcaaatatct
    18121 aaccttggca ggtcttgtcc tgggtacagg agttttattg agcgcgtgtg gaaattctag
    18181 cacggcgtca aaaacataca actatgttta ttcaagtgat ccatctagct tgaactatct
    18241 agcagaaaac cgcgcagcaa catccgatat tgttgcaaat ttggtagacg ggttattaga
    18301 aaatgaccaa tatgggaata ttattccatc attagcagag gattggactg tttctcagga
    18361 cggtttgacc tatacctaca aacttcgtaa ggatgccaag tggtttactt ctgagggaga
    18421 agaatatgcg cctgtaactg cccaggattt tgtgacaggt ttgcaatatg cagctgataa
    18481 aaaatcagaa gccttgtatc tagtgcagga ctctgttgct ggtttggatg actatatcac
    18541 tggtaaaaca agcgactttt caactgtcgg tgtcaaggca cttgatgacc aaacggttca
    18601 atatactttg gttaaaccag aactttactg gaattcaaaa acacttgcaa cgatactttt
    18661 tcctgttaat gcagatttcc tgaaatcaaa aggggatgat tttgggaagg cggatccatc
    18721 tagtattttg tacaatggac ctttcttgat gaaagcactt gtctcaaaat ctgctattga
    18781 atataagaaa aaccctaatt actgggatgc taagaatgtc tttgtagacg atgtgaaatt
    18841 gacctactat gatggtagcg accaagaatc actggaacgt aattttacag ctggtgctta
    18901 tactacggct cgtctttttc ctaacagctc cagctatgaa gggattaaag aaaaatacaa
    18961 aaacaatatc atctatagtg tgcaaaattc aacttcatat ttctttaatt ttaacctaga
    19021 taggaagtct tacaattata cttctaaaac aagtgacatt gaaaagaaat cgacccagga
    19081 agcagttctc aataaaaact tccgtcaggc tatcaatttt gcttttgaca gaacatctta
    19141 tggggctcag tctgaaggga aagaaggtgc aacaaagatt ttgcgtaacc tagtggttcc
    19201 tccaaacttt gtcagtatca agggaaaaga ctttggtgaa gttgtagcct ctaagatggt
    19261 caactatggt aaggaatggc aaggtatcaa ctttgcggat ggtcaagacc cttactacaa
    19321 tcctgagaaa gccaaggcta agtttgcgga agctaagaaa gaactcgaag caaagggtgt
    19381 tcaattccca atccacttgg ataagactgt ggaagtaaca gataaagtag gcatacaagg
    19441 agttagttct atcaaacaat caattgaatc tgttttaggt tctgataatg tagtgattga
    19501 cattcagcaa ttaacatcag atgagtttga cagttcaggc tactttgctc aaacagctgc
    19561 tcagaaagac tatgatttat atcatggcgg ttggggacct gattatcaag acccgtcaac
    19621 ctatctcgat atttttaata ctaatagtgg aggagttcta caaaatcttg gactagagcc
    19681 tggagaagcc aatgacaagg ctaaggcagt tggactggat gtctatactc aaatgttgga
    19741 agaagctaat aaagagcaag atccagccaa acgttatgag aaatatgctg atattcaagc
    19801 ttggttgatt gatagttctt tagttcttcc aagtgtttcg cgtgggggaa caccatcatt
    19861 gagaagaacc gtaccatttg ctgctgccta tggtttaacc ggtacaaaag gggttgaatc
    19921 atataaatac ctcaaagtac aagataagat tgtcacaaca gacgaatatg caaaagccag
    19981 agaaaaatgg ttgaaagaaa aagaagaatc caataaaaaa gcccaagaag aattggcaaa
    20041 acatgtcaaa taaaatctaa ttgtagataa gtttgtgtaa gaagtctttg tgcttaaaaa
    20101 cgcataatat caggtatata aaatcctaga tgttatgcgt tttgttatag gaagatttat
    20161 tagattttat cctcaaattg agttattccc gactttatcc gttgaaatat tataaggagt
    20221 atttatgaaa ttaaaattat taagagtaga tactaaggtg attatgggga gtttcttact
    20281 tgttctgtct agtctacttg ctttgttgct tccccttatc ttaaagggtt taatagatgg
    20341 gagttctatt gaaaatatag gttccaaagt atttcaatcg tttttgattt ttattggtca
    20401 agccttgttt tcttctattg gttactatct gtttagtcaa tcgggtgaaa aaagatagca
    20461 aaaatcagga aaaaagtgat agaggggttg atttatgcag agaaatcctt ctttgataag
    20521 agccaaagtg gggagttgac ttctgccatt gtcaatgaca tgagtgtcat tcgtgagttt
    20581 ttaattacga ctttcccaaa tattattctg agtttagtta tggtacttgg ttccattgta
    20641 gtcttattta gtcttgattg gaatctttct ctacttttat tcatcactct tccttgtatg
    20701 atgtttatta tcttgcccct ttccaatatc agtgaaaagt atagtcgtcg tttacaggag
    20761 gaaatcggat ttttaacagg gcaattgact gaaaagattc aagaacatga attaattaag
    20821 actaaccaag cagagaaaag tgtacaggat gttttggata attgtattga aagggtacaa
    20881 aacaattcac tgaaatcaga tagggttact tcttttgaga ccccgtttgc tctcttattt
    20941 atctttgcga ctatagttgt gatgctaacc tatgggggtt atcgggtcag cgcaggatat
    21001 atatctgtgg gaaccttggt ttcgtttttg atttacctct ttcaattact taatcctatt
    21061 agtaatatag ctaattttgt aactatttat tctagtagca agggatcttc agttgcactg
    21121 gagaacttgc ttgcagttcc taaagaaaaa tttgagggag gaaaatcggt atcaggacaa
    21181 gggttgaatt ttaaccatgt ctattttggt tatgatgaaa atcgacctgt cttaaaggat
    21241 attacttgtt caattttcaa ggggcaaaaa attgcttttg ttgggccatc tggatcagga
    21301 aaatcaacga ttgtgcgttt gttagagcgg ttttataaac cgctttcagg agatattcta
    21361 atggagcaat caagtatata tgattttaac ttaaaagaat ggagaagtaa aatcgcttgg
    21421 gtttcacaaa ataatgcagt cttatctggc agtattcgtg acaatctttg tctcggtttg
    21481 aatcgcttag taactgatga tgaattgatg aaagtgctag acttagtatc actaggtgat
    21541 gagattcgct ccatgaaaga gggactagat actgaagttg gtgaacgcgg acgactcttg
    21601 tcaggggggc gaagccaaag acttcaaata gctagagcct acttaaaaga tgctgaaatt
    21661 cttatatttg atgaagccac tgctaatctt gatgcggatt ctgagtatgc gattatcagt
    21721 agcctctatt ctgtactaaa ggagaagacg gttgtgatta tagcgcatcg cttgtcaacg
    21781 gtaaaagatg tggattgtat tttcttctta gaggagggga aaatcactgg ctcaggaact
    21841 cataaggaac tactggaaaa tcatgagcgt tatgctcgtt ttgtgcagga gcaaatgata
    21901 gagtgaaggt gtcttttgag attcaccatt ttatagtcta ttaaagggag caggaaaaac
    21961 tcccttttta tatagtttga aactataact agctcttgaa aagaagaaaa tgagttgatg
    22021 aaaataagtg gtacaatagt tactatagat ttggaggaat tgtatgagca aggaattaca
    22081 cattaacaca attttagcac aagcagggat caagtctgat gaggcaacag gtgctttggt
    22141 gacaccactt catttttcaa cgacctatca gcatccagag tttggtcgat ctactgggtt
    22201 tgactatacg cgcactaaaa atccaactcg tagtaaggct gaggaagtct tggcggctat
    22261 tgagtcagca gactatgccc tagcgactag ctcagggatg tcagctattg tactggcctt
    22321 tagcgtcttt ccagtaggaa gtaaggtctt ggcagtgcgt gatctttacg gtggttcttt
    22381 tcgctggttt aaccaagtgg agcaggaagg tcatttccat tttacctatg ccaatacaga
    22441 agaagagttg attgccgagt tagaaaagga tgtggatgtt ctctatatcg aaaccccaac
    22501 caatcccttg atgttggaat ttgatatcga aaaactagca aaattggctc atgctaaggg
    22561 tgccaaagtg gtggtggaca ataccttcta tagccctatc taccaacgtc cgattgaaga
    22621 tggagcagat atcgttctcc attcagcaac caagtatcta gcaggccaca atgatgtctt
    22681 ggctggagtg gttgtgacca atagtttaga actatacgag aagctttttt acaatcttaa
    22741 tacaacaggg gcagtcttgt ctccatttga cagctaccag ttgcttcgtg gtctcaagac
    22801 cttgtctctt cgtatggagc gctcaacagc taacgcccaa gaagtggttg cctttttgaa
    22861 ggattctcca gcagttaagg aagttctcta cactggtcgt ggaggcatga tttcctttaa
    22921 agtagccgat gaaacacgca ttcctcatat tttgaacagt ctcaaggtct tctcttttgc
    22981 ggaaagtttg ggtggagtgg aaagtcttat tacttatcca acgactcaaa ctcatgctga
    23041 tattccagca gaagtacgcc attcttatgg tttgacagat gacctcttgc gtttgtctat
    23101 tgggattgag gatgctagag atttgattgc agatttgcgc caagccttag aaggataaga
    23161 caaagatggg aaaatatgat tttacaagcc tgcccaaccg tttagggcac catacctata
    23221 aatggaaaga aacagaaacg gatagtgaag ttctaccagc ttggatagcg gatatggact
    23281 ttgtagtctt gcctgaaatc caccaagccg tgcaaactta cgcagaccaa ctggtttatg
    23341 gttataccta tgccagtgaa gacttaatta aggaagttca aaagtgggaa gctacacaat
    23401 acggttacaa ctttgacaaa gaggctcttg tctttatcga gggtgtggta ccagccatct
    23461 caacagctat tcaaaccttt acaaaagaag gcgaggcggt tttaattaac acgcctgtct
    23521 acccaccctt tgctcgcagt gtcaagttga ataatcgtag attgattact aattccttag
    23581 tggaaaagga tagtctgttt gagattgact ttgaccaact tgaaaaggat ttggtggaag
    23641 aggaggttaa actctatgtt ctttgcaacc ctcacaatcc tggtggacgt gtttgggaaa
    23701 aagaagtgtt ggagaagatt ggccaactct gccaaaaaca cggtgttttg ttagtttcgg
    23761 atgagattca ccaagatttg accctctttg gtcacaaaca ccagtctttc aataccatca
    23821 atcctgcctt caaaaatttt gctatcgtct tgagcagtgc cactaaaaca tttaatattg
    23881 ctggaacaaa aaattcctat gcagtcattg aaaatcctaa gttgagactg gctttccaga
    23941 aacgcctgtt ggccaataat cagcatgaaa tttcaggctt gggttatttg gcgacagaag
    24001 ctgcctatcg atacggtaaa gattggctag aggaactcaa gcaagtcttt gaagaccaca
    24061 tcaattatgt ggtggatcta tttggaaaag agactaaaat caaggtcatg aaaccgcaag
    24121 gtacctactt gatttggctt gacttttcag cttatgacct gactgatgaa acattgcaag
    24181 agttgttgag aaatgaagcc aaggttatcc tcaaccgtgg tttggatttt ggagaggaag
    24241 gaagtctcca tgcccgcatc aatatagcta tgcccaaatc tctgttgcaa gaagtctgtc
    24301 agcggattgt ggctactttt gccaaatgtt aaaaatccag ccttctagga gaaaagtctt
    24361 cctagaaggc tattttcata ggcgaaaata tggtataata aaaagataag gtaaaggtga
    24421 aaatatggct aaattgattc cggggaaaat tcgtatcgaa ggtgttgccc tttatgaaac
    24481 tggtaaggtt gatatcatca aggaaaagaa caatcggctc tacgctcgcg ttgcagaaga
    24541 agaactgcgc tatagtttag aggatgattt gattttttgt gcctgtgatt tttttcaaaa
    24601 gaggggctac tgtgtgcatt tggcagcgct agagcatttt ctgaaaaatg atgagcgtgg
    24661 tcaggaaatc ttgtggagtc tggaagaagg tcatgaagaa aaagaggccg ttgaaaccaa
    24721 ggtgaccttg ggtggcaagt ttttggatcg aattttatct ccaaaatcag aatgcgccta
    24781 tgagttatca gctgtgggtc aggtggaagc aggaaccaat catatcttgt ggactctgag
    24841 aatcggccaa atcaatagcc aaaaatacta tgtcattcgg gatattccac tttttttaag
    24901 gattgtcgag cagagaaagt cgtatatgat tggcaagatt tatgaggagt ctctttcttg
    24961 ggaagccttt gatgatgcca gccaagaact tttgactttc ttgcacggac taacggagga
    25021 agggctggtt ccagacctct ttttccaaaa ccaaggtcgg cacctctttt tccctctgac
    25081 cttttttgag cagggtgtga atttactgat ggccttgccc cattttcaat ttgaccatca
    25141 agtggatagc taccaaaccc tgctttttca ggatatgcat gctgatgcca atctatttgc
    25201 ctttacagta acagaatatt cggactattt tgaaatggaa atcagtgaga gtcctagggt
    25261 caatgtcttt tatcaaggag cagtactttt ccataaaggt caggtttatt ttttaacaga
    25321 ccagcagatg cgtcttctca aggaaattaa agcgttacct gtggatcgac aaggaaagaa
    25381 atacctgcaa tttgatagca gtgatcgaga caaattggcc tcctgtctga ctctttttat
    25441 ccagatgggc accgtttcag ctccagaacg tctacaaatc aaaacttttg cgccctcttt
    25501 ttactttgac agggaagaag ataatcggat tcgtttagaa attcagtttg attatggaaa
    25561 tagacaggta tctagccgac aagagctaga agaattacca ttttcaagtg acgcggactt
    25621 agaagaaaga attttccaag tctgtttggc tgcgggcttt gaagccgatt ttcaatcttg
    25681 gcgtcaggcc ttaaaagcgg agtctgtcta tcattttttt catgatatca ttccagtctt
    25741 tgaaaaactc gggcatgttg acctatcaga taaactagaa gagctttata gcctagcgag
    25801 tcctcaggtg cagattgcct ccaagggagg tctcttggaa atccagtttg attttcaaga
    25861 tattgcccag gaggaaatcg accaagccat acaggccttg gttgccaatc aggattttta
    25921 tattgattcg tctaatcaag tttacttttt cgatgaagaa accaagaaaa ttcgccaaaa
    25981 tctacaggaa ctggggcaat ttgaattaaa agatgggacc ttgcaggctc ggaaatcctt
    26041 ggcctacagt ttagctcatc tctttgaagg gcgagaccgt gtttcttttt cacaagaatt
    26101 ccagaatctg gcccaggatt tgacgcatcc agaggacttt cctttgcaag caactcaggt
    26161 caaggctgac ttgcgagatt atcaggaaaa gggaattggc tggttgcaga tgctccatca
    26221 ttatggtttt ggtgggattt tggctgatga tatgggactt gggaaaaccc ttcagaccat
    26281 tgcttttttg actagtcaag tgacaaaaga aagtcgggtt ttgattctcg ctccgtcggg
    26341 tttgatttac aactgggcag atgagtttca gaaatttgct ccacagttgg atgtggctgt
    26401 tgttcatggt ttgaaagcaa gtcgtgagga gattcttgcc gagagccatc aaatctatgt
    26461 gactagctat gccaccttcc gtcaggacag tgagctttat caggggatgg cctttgactt
    26521 ccttttctta gatgaagctc aggtcatgaa aaatgcccag accaagattg cccagacctt
    26581 gagacaattt gtggtgccgt cggtctttgc cttgtcagga actccgattg aaaaccatct
    26641 gggtgagttg tggtctattt ttcaaatcgt catgcccgga cttttgccaa gcaagaaaga
    26701 atttatgaaa ttgccagcag agcgagtggc tcagtttatc aagcctttcg tgatgcgacg
    26761 caagaaagaa gaagttctga ctgaattgcc agacttgatt gaggtggttt ataaaaatga
    26821 actggaagac cagcaaaagg ctatttacct tgcccagtta caacagatgc gagaccatct
    26881 ggctcaagtg tcagaacagg aatttcagcg aagtcgtgtg gaaattttat ctggtttgat
    26941 gcgcttgcgt caaatatgtg acactcctgc cctgtttatg gaagattatc agggagccag
    27001 cggcaaacta gatagtctcc gagacctact ggtacaggtg gcagacggcg gacaccgtgt
    27061 cttgattttc tctcagttca agggaatgtt ggaaaaaatt gaacaagaac tgccagactt
    27121 gggcctgaca tcctttaaaa ttacgggttc aaccccagcc aaggaaagac aagacatgac
    27181 caaggccttt aaccaaggag aaagagatgc ctttctgatt tccctcaagg ctggtggggt
    27241 cggtctgaac ctgacaggtg cagatacagt gatcttggtt gacctttggt ggaatcctgc
    27301 ggtggaagcg caagccattg gccgtgccca tcggatgggt caggaagaaa cggttgaggt
    27361 ctatcgcttg gtgaccaagg gaaccattga agaaaaaatt caggaactcc aagaacaaaa
    27421 gaaacatctg gtgtcacaag tattggacgg cacagagtca cgtggtagtc tgaccctagc
    27481 agaaattaga gaaattttgg gaatttctga agccagcact tgaaaaatca agacaatacg
    27541 ctataatgaa gtgttgaaag gaaatagaaa atgaaacaag aacgatttcc attggtgtca
    27601 gatgacgagg tcatgttgac tgaaatgcca gtcatgaatc tctatgatga gtctgatctg
    27661 atcagtaata tcaagggtga gtatcgagat aaaaattatt tagaatgggc tcctattgct
    27721 gaagaaaaac cagtaaaacc gattgaaaag caagtaggga aacctaaaaa agctccttta
    27781 ggggttaaaa aagaaggaaa gagctatgcg gaggtggcgc gtgaagaagc gcgtgcagac
    27841 ttgaaaaaga aacgctctgc taactatcta actcaggatt tcagccttgc gagacgtcat
    27901 tctcagccca gtctagttag acagggcaat caaccgacaa ctcctttcca aaaggaaaat
    27961 cctggtgaat ttgtcaaata tagccaaaaa ttgacccagt ctcattatat cttggcggaa
    28021 gaagttcatt ctatccctac caagaatgaa gaagtgtcag cacctgctcc aaagaaaaac
    28081 aattatgatt ttctaaagaa gagccaaatc tacaataaaa aaagtaaaca aacagaacaa
    28141 gaacgtcggg ttgcccaaga gttgaatctg accagaatga cagaataggg gagaaaacat
    28201 gtcaaagaca tatcatttta tcggaattaa gggatcaggg atgagtgcct tggccttgat
    28261 gttgtaccag atggggcaca aggttcaggg atcagatgtt gaaaagtact actttaccca
    28321 acgcggtctt gagcaggcag gaattaccat tcttcctttt gatgaaaaga atctagacgg
    28381 tgatatggaa attatcgctg gaaatgcctt tcgtccagat aacaacgtcg aaattgccta
    28441 tgcggaccaa aatggtatca gctacaaacg ttaccatgag tttctaggta gctttatgcg
    28501 tgactttgtt agcatgggag tagcaggagc acatggaaaa acttcaacga caggtatgtt
    28561 gtctcatgtc ttgtctcaca ttacagatac cagcttcttg attggagatg ggacaggtcg
    28621 tggttcggcc aatgccaaat attttgtctt tgaatctgac gaatatgagc gtcacttcat
    28681 gccttaccac ccagaatact ctattatcac caacattgac tttgaccatc cagattattt
    28741 cacaagtctc gaggatgttt tcaatgcctt taacgactat gccaaacaaa tcaccaaggg
    28801 tctttttgtc tatggtgaag atgctgaatt gcgtaagatt acgtctgatg caccaattta
    28861 ttattatggt tttgaagctg aaggcaatga ctttgtagct agtgatcttc ttcgttcaac
    28921 aactggttca accttcaccg ttcatttccg tggacaaaac ttggggcaat tccacattcc
    28981 aacctttggt cgtcacaata tcatgaatgc gacagccgtt attggtcttc tttacacagc
    29041 aggatttgat ttgaacttgg tgcgtgagca cttgaaaaca ttttccggtg ttaaacgtcg
    29101 tttcactgag aaaattgtca atgatacagt gattatcgat gactttgccc atcatccaac
    29161 agaaattatt gcgaccttgg atgcggctcg tcagaaatac ccaagcaagg aaattgtagc
    29221 agtctttcaa ccgcatacct ttacaagaac cattgccttg ttggacgact ttgcccatgc
    29281 tttaaaccaa gcagatgctg tttatctagc gcaaatttat ggctcggctc gtgaagtaga
    29341 tcatggtgac gttaaggtag aagacctagc caacaaaatc aacaaaaaac accaagtgat
    29401 tactgttgaa aatgtttctc cactcctaga ccatgacaat gctgtttacg tctttatggg
    29461 agcaggagac atccaaacct atgaatactc atttgagcgt ctcttgtcta acttgacaag
    29521 caatgttcaa taggatgttc ccatggaaat tccaattaag atcattcagg caagcaagtc
    29581 tgatttgcct gagatagggg cacttcagac ctcgtctttt ccagctgaaa agcagcaact
    29641 ttcccatatt ttagaagaaa gtatccgtaa gtgtgcggat acctttcttc tagctaggga
    29701 tgaaaatcaa cttttaggct atattttatc aagtccccag tcagacaatc cgcaatatct
    29761 aaaagtacat tctttagtca tcgagtctga ccatcagaga cagggcttgg gaacacttct
    29821 tcttgcagcc ttgaaagagg tggcagttga gctggattac aaagggattc gtttggagag
    29881 tcctgatgag ctgctttcct attttgaaat gaacggtttt gttgatgaag aagcaacttt
    29941 gctctatgca actagccagg gctatagtat gatttggttt aatccctttt atctggagga
    30001 acaatgaaaa tcagacaagc aagattagaa gatttggatc ggattgttga acttgaattt
    30061 gaaaattttt cggtcgaaga agccattcct ccttctgtct ttgaagcaca tttgagagaa
    30121 attcagacct cttttctggt tgctgaaaaa gaaggaagaa tcatgggtta tatcgaagga
    30181 ccagttggcc tgcaccgcca tctgcaagac cagtctttta cagaagaaat aaaagactat
    30241 agtcatgagc ctggtggtta tatctctgtg acctgtctgt ctattgccaa ggaggcacag
    30301 ggactcggac tgggtcagaa attgctgaca gccttgaaag aagttgctct tgaagatgag
    30361 agagatggca ttaatctaac ctgtcatgac tatctcatcg cctactatga aaaacatgga
    30421 tttgtcaacg aaggccagtc ccagtcaacc tttgcagggg aaacatggta tgatatggtc
    30481 tgggaaatga aaaaataagt taggaaaagt atcatagatt gctttttttg gacttttaag
    30541 atataatatt atggattatc aacaaggagg aaaaactttt gagtgaaaag tcaagagaag
    30601 aagagaaatt aagctttaaa gagcagattc tgagagattt agaaaaagta aaaggctatg
    30661 atgaagttct gaaagaagat gaggcagtag ttcgcactcc tgcaaatgaa ccttcaactg
    30721 aagaactcat ggctgattcc ttgtcaacgg tagaggagat tatgagaaaa gctcctaccg
    30781 tgcctactca cccaagtcaa ggtgtaccag cttctccagc agatgagatt caaagagaaa
    30841 ctcctggtgt tccaagtcat ccaagtcaag atgtaccttc ttctccagcg gaagaaagtg
    30901 gatcaagacc aggtccaggt cctgttagac ctaagaaact tgaaagagaa tacaatgaaa
    30961 ccccaacaag ggtagctgtt tcctatacga cggcagagaa aaaagcagaa caagcaggtc
    31021 cagaaacacc tacgcctgct acagaaacag tggatatcat cagagataca tcacgtcgta
    31081 gccgtagaga aggagcaaaa cccgttaagc ctaagaaaga gaagaagtca catgtgaaag
    31141 cttttgtgat ttcattcctt gtattccttg ccttgctctc agcaggtggt tactttggtt
    31201 accagtacgt gctagattcc ttattaccta tcgatgctaa ttctaagaaa tatgtgacgg
    31261 ttggaattcc agaaggttca aacgttcaag aaatcggtac gacgcttgaa aaagctggtt
    31321 tggtaaagca tggtctgatt tttagttttt atgccaagta taaaaattat accgacttga
    31381 aagcaggtta ctacaatttg caaaagagta tgagtacaga agacttactc aaagagttgc
    31441 aaaaaggtgg aacagatgaa ccgcaagaac ctgtacttgc gactttgaca attccagaag
    31501 gttatacctt ggatcagatt gctcaagctg tgggtcaatt gcaaggtgac ttcaaagagt
    31561 ctttgacagc ggagactttc ttggctaaag ttcaagatga gacgtttatc agtcaagcag
    31621 tagcgaaata tcctacttta ctggaaagtt tgcctgtaaa agacagcggt gcgcgttatc
    31681 gtttggaagg ataccttttc ccagctacat actctatcaa ggaaagcaca actattgaga
    31741 gcttgattga tgagatgtta gctgctatgg ataagaacct atctccttac tatagtacta
    31801 tcaaatctaa aaacttgact gtcaatgagt tgttgaccat tgcttccttg gtcgaaaaag
    31861 aaggtgccaa gacagaagat cgtaagctca ttgcaggtgt attctacaat cgtttgaatc
    31921 gtgatatgcc acttcaaagt aatattgcaa tcttgtatgc ccaaggaaaa ctggggcaaa
    31981 atatcagtct agctgaggat gttgcgattg ataccaacat tgattcacct tataatgttt
    32041 ataaaaatgt aggtctcatg cctggtccag tcgatagtcc aagtctggat gcgattgagt
    32101 caagcatcaa tcaaactaag agcgataacc tctactttgt agcagatgtc acagaaggca
    32161 aggtctacta tgctaacaat caagaagacc acgaccgcaa tgtcgctgaa catgtcaaca
    32221 gcaaattaaa ctaaacaaac taaaattatg ttatacttca cataattttc tttagaaaat
    32281 attatcagaa gaaagttgag aaaaatggca gaaaaaacat atcctatgac ccttgaggaa
    32341 aaggaaaaac ttgaaaaaga attagaagaa ttgaaattgg ttcgtcgacc agaagtggta
    32401 gaacgcatta agattgcccg ttcatacggt gacctttcag aaaacagtga gtacgaagca
    32461 gctaaggatg aacaagcctt tgtcgaagga caaatctcta gcttagaaac aaaaatccgc
    32521 tatgctgaaa tcgtcaatag cgacgcagtt gcccaggacg aagtagcgat tggtaaaaca
    32581 gtcaccatcc aagaaattgg tgaggacgaa gaagaagttt atattatcgt aggttcagct
    32641 ggtgcggatg cctttgcagg taaggtttca aatgaaagcc caattgggca ggccttgatt
    32701 ggcaagaaaa caggtgatac agcaaccatt gaaacgcctg ttggtagcta tgatgtaaaa
    32761 atcttgaagg ttgaaaaaac agcctaaaaa cagaaaaagg agtggggagg cgatgtgctt
    32821 cactcactcc tttttccatt ttgctattct tcgaaaatct cttcaaacca cgtcagcgtc
    32881 gccttgccgt atgtatggtt actgacttcg tcagtttcat ctacaacctc aaaaccatgt
    32941 tttgagccga cttcgtcagt ttcatctaca acctcaaaac catgctttga gctgacttcg
    33001 tcagtttcat ctacaacctc aaaaccatgc tttgagctga cttcgtcagt ttcatctaca
    33061 acctcaaaac catgctttga gctgacttcg tcagttctat ctacaacctc aaaacagtgt
    33121 tttgagcaac ctgcggctag tttcctagtt tgctctttga tttttattga gtattagatg
    33181 tatagtgcat tgaagttaaa atagtacact ttgatttcta agacattgtt agaaattaga
    33241 ttaaattcct taatcgattt gcgcatgttt tatttcattt cactatactt tagattggaa
    33301 aataaggaat cacataaatc gcaagaaata gggaatctga ttcgtgttta taaccgttca
    33361 aaaagagaag aggctgaaag tgagccactt aatctttatg tcgaagatga aaagggcaat
    33421 ctcctggcag gtttgatagc agagactttt ggaaattggc tagaaatcga gtatttgttt
    33481 gtaaaagagg aactgcgagg gcaaggaatc ggttcaaaac tattgcaaca ggcagaaagt
    33541 gaagctaaga atcgaaattg ttgttttgcc tttgtcaata cttaccagtt ccaggcaccg
    33601 gacttttatc agaaacatgg ctacaaggaa gttttttctt tgcaagacta tctctacatt
    33661 aggcaaagat attattacca aaagaatctg taaggtgagt atggaggtat aatacaaaga
    33721 gaggctcgca cataagtatg gaggtggact gatgtatatg agtaactatc gataaatacc
    33781 agttataacc tagaaattgt gtctttctag acgatattga ggacaatata atcgtagccc
    33841 caaaattagg aatcaagact tatcaggtta agaaaagaag tgatgttgtt gatattttaa
    33901 acaatttatt taaactaaaa actctctatc tattaatccg agattcccct ctcggatttt
    33961 gtgttgcttt cattaaattt acaggtaaga cttgctagct tcagtaaata tacacaagtt
    34021 tttcagaata gagtgaaatc caaaagaaaa tggaaataat gaaagctctt gtaggcataa
    34081 gaaaacacct ctgtgttata cttgttgttc aaccacaaac acaagaaagg cacagagatg
    34141 caagacaatt atactacaaa agccaaacat ttgacaatcg atagccgtcg cttaatcgaa
    34201 agatggaaaa aagaagggaa atcaaataga gaaattgcct ctctgcttgg aaaagttcct
    34261 caaactatcc acactgaaat caagcgtggg acagtccgac aatgtcttgg aaaagggcgc
    34321 ttcaaagagg tttattctgc cgactatgct caacagtctt atgaaaataa tcgcaagcgc
    34381 tcggtcaaga aatcaagctt gaccaaggaa ctaaaggaaa agattctcca ctatcataac
    34441 caaaaatttt cgcctgaaat gatggtgatg gctaaagggg ttaacgtggg aatttcaacc
    34501 atttactatt ggattcatca tggaaaattg gggttaagca aacaggatct gctttaccct
    34561 agaaaaggaa aagcgcttaa gaaacaggct agcaccaact ttaaacctgc tggtcaatcc
    34621 atcgaacagc ggtctgaagc tatcaatctt cgcttggaga atgggcatta tgagattgat
    34681 acggttctac ttacgagagc gaaaaactac tgcttgcttg tcttgacgga tcgaaagagt
    34741 agacatcaga tcatccgatt gattccaaat aaaagtgctg aggtggtcaa tcaggctcta
    34801 aaactcatct taaaacaaca caagattctt tccatcacgg cagataatgg aacggaattc
    34861 aatcgcttgt ttgatgtatt ttctgaggag cacatctatt atgcgcaccc ctatgcctct
    34921 tgggaaaggg gaactaatga gaatcacaac aggctcattc gtagatggtt acctaaggga
    34981 accaagaaaa tgactcccaa agaagtcgca ttcatcgaaa agtggattaa caactatcct
    35041 aaaaaatgct tggactacaa gtcacccaga gaagacttct ggatggctaa cttgaacttg
    35101 aaatttagca aaatggaaat aatttttatt aaacgcttcc aatagcggtt aaaagttgac
    35161 aaatttaaat tttaggacta cactaagtaa gtaaatttta ttttaaagga gaatttgtta
    35221 tgaatttaac atttttaggc ttatgtattg cctgtatggg cgtatctgtc ggtgaaggtt
    35281 tattgatgaa tggactgttt aaatcagtag cacgccaacc agatatgctt tctgagtttc
    35341 gtagtttgat gtttttaggt gttgccttta ttgaaggaac tttctttgta actcttgtct
    35401 tctcatttat tatcaaataa atacatggaa cgagaagaaa agggaggatt ttagatggaa
    35461 gaaagtatta atccaatcat ctctattggt cctgttatct tcaatctgac tatgttagcc
    35521 atgactttgt tgattgtggg agttattttt gtctttattt attgggcaag ccgcaatatg
    35581 accttgaaac ccaaaggaaa gcaaaatgta cttgagtatg tctatgactt tgttattgga
    35641 tttacagaac ctaacattgg ttcgcgctac atgaaagatt actcactctt tttcctttgt
    35701 ttattccttt tcatggtgat tgccaataac cttggcttaa tgacaaagct tcaaacgatc
    35761 gatgggacta actggtggag ttcgccaacc gctaatttac agtatgactt aaccttatct
    35821 tttcttgtca ttttgttgac acatatagaa agcgttcgtc gtcgtggatt taaaaaaagt
    35881 ataaaatctt ttatgagtcc tgtttttgtc ataccgatga atatcttgga agaatttaca
    35941 aacttcttat ctttggcttt gcggattttt gggaatatct ttgcaggaga ggtcatgacg
    36001 agtttgttac ttcttctttc ccaccaagct atttattggt atccagtagc ctttggagct
    36061 aatttggctt ggactgcatt ttctgtcttt atttcctgca tccaagctta tgtttttact
    36121 cttttgacat ctgtgtattt agggaataag attaatattg aagaggaata gaaaggagta
    36181 actgatgcac gtaacagtag gtgaattaat tggtaatttt attttaatca ctggctcttt
    36241 tattcttttg ctagtcttga ttaaaaaatt tgcatggtct aatattacag gcattttcga
    36301 agaaagagct gaaaaaattg cttcagatat tgacagagct gaagaagccc gtcaaaaagc
    36361 agaagtattg gctcaaaaac gcgaagatga attggctggt agccgtaaag aagctaagac
    36421 aatcattgaa aatgcaaagg aaacagctga gcaaagtaag gctaatatct tagcagatgc
    36481 taaactagaa gcaggacact taaaagaaaa agccaatcaa gaaattgctc aaaataaagt
    36541 agaagcttta cagagtgtta agggtgaggt cgcagatttg accatcagct tagctggtaa
    36601 aatcatctca caaaaccttg acagtcatgc ccataaagca ctcattgatc agtatatcga
    36661 tcagctagga gaagcttaat ggacaagaaa acagtaaagg taattgaaaa atacagcatg
    36721 ccttttgtcc aattggtact tgaaaaagga gaagaagacc gtatcttttc agacttgact
    36781 caaatcaagc aagttgttga aaaaacaggt ctgccttctt ttttaaaaca agtggcagta
    36841 gacgagtcgg ataaggaaaa aacaattgct tttttccaag attctgtgtc gcctttatta
    36901 caaaacttta tccaggttct ggcctacaat cacagagcaa atctttttta tgatgtgctt
    36961 gtagattgct tgaaccgact tgaaaaagaa acaaatcgat ttgaagtgac gattacgtct
    37021 gctcatcctc taactgatga acagaagact cgtttgctcc ctttgattga gaaaaaaatg
    37081 tctctgaaag taaggagtgt aaaagaacaa atcgatgaaa gtctcattgg tggttttgtc
    37141 atttttgcca atcacaagac aattgatgtg agtattaaac aacaacttaa agttgttaaa
    37201 gaaaatttga aatagaaagt ggtgttcttt tggcaattaa cgcacaagaa atcagcgctt
    37261 taattaagca acaaattgaa aatttcaaac ccaattttga tgtgactgaa acaggtgttg
    37321 taacctatat cggggacggt atcgcgcgtg ctcatggcct tgaaaatgtc atgagtggag
    37381 agttgttgaa ttttgaaaac ggctcttatg gtatggctca aaacttggag tcaacagacg
    37441 ttggtattat catcctaggt gactttacag atatccgtga aggcgataca atccgccgta
    37501 cagggaaaat catggaagtc cctgtaggtg aaagtctgat tggtcgtgtt gtggatccgc
    37561 ttggtcgtcc agttgacggt cttggagaaa tccacactga taaaactcgt ccagtagaag
    37621 caccagctcc tggtgttatg caacgtaagt ctgtttcaga accattgcaa actggtttga
    37681 aagctattga cgcccttgta ccgattggtc gtggtcaacg tgagttgatt atcggtgacc
    37741 gtcagacagg gaaaacaacc attgcgattg atacaatctt gaaccaaaaa gatcaagata
    37801 tgatctgtat ctacgtcgcg attggacaaa aagaatcaac agttcgtacg caagtagaaa
    37861 cacttcgtca gtacggtgcc ttggactaca caatcgttgt gacagcctct gcttcacaac
    37921 catctccatt gctcttccta gctccttata ctggggttgc tatggcggaa gaatttatgt
    37981 atcaaggtaa gcatgttttg attgtatacg atgatctatc aaaacaagcg gtagcttatc
    38041 gtgaactatc gctcttgctt cgtcgtcctc caggtcgtga agccttccca ggggatgttt
    38101 tctatctcca cagccgtttg cttgagcgct cagctaaagt ttctgatgaa cttggtggtg
    38161 gatcaattac agccctacca tttatcgaga cacaagcagg agatatctca gcctatatcg
    38221 caaccaacgt gatttctatc actgatggac aaatcttcct tggcgatggc ctcttcaatg
    38281 caggtattcg tccagccatc gatgcgggtt catctgtatc tcgtgtaggt ggttctgcac
    38341 aaatcaaagc catgaagaag gttgctggta cacttcgtat cgaccttgct tcataccgtg
    38401 agttggaagc ctttactaag tttggttctg acttggacgc agcaacacag gctaagttga
    38461 accgtggacg tcgtaccgtt gaggtcttga aacaacctgt tcacaaacca ttacctgttg
    38521 agaaacaagt aaccattctt tatgctttga cacatggttt cttggatact gttccagtag
    38581 atgatattgt tcgtttcgag gaagagttcc atgccttctt tgatgctcaa catccagaga
    38641 ttttggaaac cattcgtgat acaaaagact tgccagaaga agcagtcttg gatgctgcga
    38701 ttacagagtt tctcaatcaa tctagcttcc aataagaata gaggtgtcag atggcagtgt
    38761 ctctaaatga tattaaaaca aaaatcgcct caacaaaaaa tacgagtcaa atcactaatg
    38821 ccatgcaaat ggtatcggct gctaagttag gtcgctctga agaagctgct cgcaacttcc
    38881 aagtttacgc tcagaaagtg cgtaaacttt tgacagatat ccttcatggt aatggagctg
    38941 gtgcttcaac taatccgatg ttgattagcc gttctgtgaa gaagacaggc tatatcgtta
    39001 tcacttcaga ccgcggtttg gttggaggtt ataattcctc tattttgaaa gctgttatgg
    39061 agttgaaaga agaataccac ccagacggta aaggttttga aatgatctgt atcggtggga
    39121 tgggagctga tttctttaag gctcgcggta ttcaaccact ttatgaatta cgtggcttgg
    39181 cagaccaacc tagctttgat caagttcgta agattatttc aaaaactgtt gaaatgtacc
    39241 aaaatgaact ctttgatgag ctttatgttt gctacaacca ccatgtcaat acgctaacca
    39301 gtcaaatgcg tgtggaacaa atgcttccga ttgttgactt ggatccaaat gaagcggatg
    39361 aagagtacag cttgactttt gaattggaaa ccagccgaga agaaattctg gagcagttgt
    39421 tgcctcagtt tgcagaaagt atgatttacg gtgccattat cgatgccaag acagctgaga
    39481 atgctgcggg tatgacagcc atgcaaacag cgacagataa tgctaagaaa gtcatcaatg
    39541 atttgacaat tcagtataac cgtgccagac aggcggcgat tacacaagaa attacagaaa
    39601 tcgtagcagg tgctagtgcc ttagaatagg ctctagtcca gctcgtatga aaatgaactt
    39661 aggacctagt tgagctagga accgacagta tcttatatag aataggagaa ggagatgagt
    39721 tcaggtaaaa ttgctcaggt tatcggtccc gttgtagacg ttttgtttgc agcaggggaa
    39781 aaacttcctg agattaacaa tgcacttgtc gtctacaaaa atgacgaaag aaaaacaaaa
    39841 atcgtccttg aagtagcctt ggagttagga gatggtatgg ttcgtactat cgccatggaa
    39901 tcaacagatg ggttgactcg tggaatggaa gtattggaca caggtcgtcc aatctctgta
    39961 ccagtaggta aagaaacttt gggacgtgtc ttcaacgttt tgggagatac cattgatttg
    40021 gaagctcctt ttacagaaga cgcagagcgt cagccaattc ataaaaaagc tccaactttt
    40081 gatgagttgt ctacctcttc tgaaatcctt gaaacaggga tcaaggttat tgaccttctt
    40141 gccccttacc ttaaaggtgg taaagttgga cttttcggtg gtgccggagt tggtaaaacc
    40201 gtcttaatcc aagaattgat tcacaacatt gcccaagagc acggtggtat ttcagtattt
    40261 actggtgttg gggaacgtac tcgtgagggg aatgaccttt actgggaaat gaaagaatca
    40321 ggcgttatcg agaaaacagc catggtcttt ggtcagatga atgagccacc aggagcacgt
    40381 atgcgtgttg cccttactgg tttgacaatc gctgaatact tccgtgatgt ggaaggccaa
    40441 gacgtgcttc tctttatcga taatatcttc cgtttcactc aggctggttc agaagtatct
    40501 gcccttttgg gtcgtatgcc atcagccgtt ggttaccaac caacacttgc tacggaaatg
    40561 ggtcaattgc aagaacgtat cacatcaacc aagaagggtt ctgtaacctc tatccaggct
    40621 atctatgtgc cagcggatga ctatactgac ccagcgccag caacagcctt cgctcacttg
    40681 gattcaacaa caaacttgga acgtaaattg gtacaattgg gtatctaccc agccgttgac
    40741 ccacttgctt caagctcacg tgccttggca cctgaaatcg ttggagaaga gcactatgca
    40801 gttgctgctg aagtaaaacg tgtccttcaa cgttaccatg aattgcaaga tatcattgct
    40861 atccttggta tggatgagct ttctgatgaa gaaaagacct tggttgctcg cgcccgtcgt
    40921 atccagttct tcttgtcaca aaacttcaac gttgcggaac aatttactgg tcagccaggt
    40981 tcttatgttc cagttgctga aactgtacgt ggctttaagg aaatccttga tggtaaatac
    41041 gaccacttgc cagaagatgc cttccgtggt gtaggttcta tcgaagatgt gattgcaaaa
    41101 gctgaaaaaa tgggatttta agaggtgacc tatggctcag ttaactgtcc agatcgtgac
    41161 accagatggt ctcgtctatg atcaccatgc cagctatgta tcggttcgaa ctctggatgg
    41221 tgagatgggg atcttgccac gacatgaaaa tatgattgcg gttttagcag ttgatgaagt
    41281 aaaggtaaaa cgtatcgatg ataaagatca cgtgaactgg attgcagtaa acggtggcgt
    41341 tattgaaatt gccaatgata tgatcacaat cgtcgctgac tctgcagaac gtgctcgtga
    41401 tatcgatatt agccgtgcag aacgtgccaa acttcgtgca gaacgtgcaa ttgaagaagc
    41461 acaagacaaa catttgattg accaagaacg tcgtgctaag attgctttgc aacgtgccat
    41521 taaccgtatt aatgtcggaa atagactata agaaaaaatg aacttgaaaa taccaagttc
    41581 attttttagg ctctttgtca actgtagtgg gttgaaaaa
//
LOCUS       DAVKFH010000013        40225 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKFH010000013 DAVKFH010000000
VERSION     DAVKFH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.013"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            5..75
                     /locus_tag="VW006_000720"
     tRNA            5..75
                     /locus_tag="VW006_000720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VW006_000721"
     tRNA            110..183
                     /locus_tag="VW006_000721"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VW006_000722"
     tRNA            266..355
                     /locus_tag="VW006_000722"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VW006_000723"
     tRNA            366..439
                     /locus_tag="VW006_000723"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VW006_000724"
     tRNA            443..515
                     /locus_tag="VW006_000724"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VW006_000725"
     tRNA            528..608
                     /locus_tag="VW006_000725"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VW006_000726"
     tRNA            613..683
                     /locus_tag="VW006_000726"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VW006_000727"
     tRNA            698..770
                     /locus_tag="VW006_000727"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            784..855
                     /locus_tag="VW006_000728"
     tRNA            784..855
                     /locus_tag="VW006_000728"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ttg)
     gene            865..948
                     /locus_tag="VW006_000729"
     tRNA            865..948
                     /locus_tag="VW006_000729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Leu,seq:caa)
     gene            1055..2332
                     /locus_tag="VW006_000730"
     CDS             1055..2332
                     /locus_tag="VW006_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9379613.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2408..3892
                     /locus_tag="VW006_000731"
     CDS             2408..3892
                     /locus_tag="VW006_000731"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9379614.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLIDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3953..5233
                     /locus_tag="VW006_000732"
     CDS             3953..5233
                     /locus_tag="VW006_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9379615.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5237..5857
                     /locus_tag="VW006_000733"
     CDS             5237..5857
                     /locus_tag="VW006_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9379616.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6023..7135
                     /locus_tag="VW006_000734"
     CDS             6023..7135
                     /locus_tag="VW006_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9379617.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7458..7883
                     /locus_tag="VW006_000735"
     CDS             7458..7883
                     /locus_tag="VW006_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9379618.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     SLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7894..8253
                     /locus_tag="VW006_000736"
     CDS             7894..8253
                     /locus_tag="VW006_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9379619.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8360..9004
                     /gene="pcp"
                     /locus_tag="VW006_000737"
     CDS             8360..9004
                     /gene="pcp"
                     /locus_tag="VW006_000737"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9379620.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9032..9889)
                     /locus_tag="VW006_000738"
     CDS             complement(9032..9889)
                     /locus_tag="VW006_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9379621.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10019..11161
                     /gene="tgt"
                     /locus_tag="VW006_000739"
     CDS             10019..11161
                     /gene="tgt"
                     /locus_tag="VW006_000739"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9379622.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11259..13076
                     /locus_tag="VW006_000740"
     CDS             11259..13076
                     /locus_tag="VW006_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9379623.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13229..14380
                     /gene="nagA"
                     /locus_tag="VW006_000741"
     CDS             13229..14380
                     /gene="nagA"
                     /locus_tag="VW006_000741"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9379624.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14543..15601
                     /locus_tag="VW006_000742"
     CDS             14543..15601
                     /locus_tag="VW006_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9379625.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15752..16117
                     /locus_tag="VW006_000743"
     CDS             15752..16117
                     /locus_tag="VW006_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9379626.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16193..17134
                     /locus_tag="VW006_000744"
     CDS             16193..17134
                     /locus_tag="VW006_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9379627.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17082..18098
                     /locus_tag="VW006_000745"
     CDS             17082..18098
                     /locus_tag="VW006_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9379628.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18100..18426
                     /locus_tag="VW006_000746"
     CDS             18100..18426
                     /locus_tag="VW006_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU9379629.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18419..18823
                     /locus_tag="VW006_000747"
     CDS             18419..18823
                     /locus_tag="VW006_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9379630.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18828..19088
                     /locus_tag="VW006_000748"
     CDS             18828..19088
                     /locus_tag="VW006_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379631.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19051..19512
                     /locus_tag="VW006_000749"
     CDS             19051..19512
                     /locus_tag="VW006_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9379632.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19490..19936
                     /locus_tag="VW006_000750"
     CDS             19490..19936
                     /locus_tag="VW006_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9379633.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            20039..20626
                     /locus_tag="VW006_000751"
     CDS             20039..20626
                     /locus_tag="VW006_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9379634.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            20687..21640
                     /locus_tag="VW006_000752"
     CDS             20687..21640
                     /locus_tag="VW006_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9379635.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21691..22881
                     /locus_tag="VW006_000753"
     CDS             21691..22881
                     /locus_tag="VW006_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9379636.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYFAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23030..23401
                     /gene="rnpA"
                     /locus_tag="VW006_000754"
     CDS             23030..23401
                     /gene="rnpA"
                     /locus_tag="VW006_000754"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9379637.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23376..24200
                     /locus_tag="VW006_000755"
     CDS             23376..24200
                     /locus_tag="VW006_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9379638.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24219..25205
                     /locus_tag="VW006_000756"
     CDS             24219..25205
                     /locus_tag="VW006_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9379639.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25256..25879
                     /locus_tag="VW006_000757"
     CDS             25256..25879
                     /locus_tag="VW006_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9379640.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26010..27467
                     /locus_tag="VW006_000758"
     CDS             26010..27467
                     /locus_tag="VW006_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9379641.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27490..27771
                     /locus_tag="VW006_000759"
     CDS             27490..27771
                     /locus_tag="VW006_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9379642.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27848..28333
                     /locus_tag="VW006_000760"
     CDS             27848..28333
                     /locus_tag="VW006_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9379643.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28350..29015
                     /locus_tag="VW006_000761"
     CDS             28350..29015
                     /locus_tag="VW006_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9379644.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29019..29882
                     /locus_tag="VW006_000762"
     CDS             29019..29882
                     /locus_tag="VW006_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9379645.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29884..30588
                     /locus_tag="VW006_000763"
     CDS             29884..30588
                     /locus_tag="VW006_000763"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9379646.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30767..32440
                     /locus_tag="VW006_000764"
     CDS             30767..32440
                     /locus_tag="VW006_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9379647.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            32552..33643
                     /gene="ulaG"
                     /locus_tag="VW006_000765"
     CDS             32552..33643
                     /gene="ulaG"
                     /locus_tag="VW006_000765"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9379648.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33757..35733
                     /gene="tkt"
                     /locus_tag="VW006_000766"
     CDS             33757..35733
                     /gene="tkt"
                     /locus_tag="VW006_000766"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9379649.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35850..36149
                     /gene="yajC"
                     /locus_tag="VW006_000767"
     CDS             35850..36149
                     /gene="yajC"
                     /locus_tag="VW006_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9379650.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36196..36624
                     /locus_tag="VW006_000768"
     CDS             36196..36624
                     /locus_tag="VW006_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9379651.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLCQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36626..37036
                     /locus_tag="VW006_000769"
     CDS             36626..37036
                     /locus_tag="VW006_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379652.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37336..39987
                     /gene="adhE"
                     /locus_tag="VW006_000770"
                     /gene_synonym="adhC"
     CDS             37336..39987
                     /gene="adhE"
                     /locus_tag="VW006_000770"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9379653.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12205 a         7208 c         9062 g        11750 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttaacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      841 atccagctac cccagttact atttgccggc gtggcggaat tggcagacgc gctggactca
      901 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      961 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
     1021 aaattttatt tagattaaga aagtgtaggg gagtatgtct tgttctatcg atttattaaa
     1081 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1141 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttatga aggatctatt
     1201 tcattattta gtttctactt tggatctcac cgtagcaaag gtagatgatt ttggcaatct
     1261 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1321 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1381 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1441 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1501 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1561 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1621 cttacgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1681 ctctgaattt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1741 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1801 agctgatttt tttgactatc taaatcattc tgcgattttt actgcggaac gtgatgggca
     1861 gacctattat ttttatccta ttcaggctgg ggactatttg gctacgtccg aaatccaagc
     1921 atttgctctg aatggggatg aggttattat ttacccccaa gagaaggatt ttgaaactca
     1981 tcgtagttac cagtaccaag atttaacgac tcgaggaaca gttgagtttc gtagtgtgtg
     2041 tacacagcca cttgatagga cttttgcttc tgcagctttt cacttgggat tattggttaa
     2101 tttagacaag ttagaagctt acttagaaac agcacctttc tttaaagtat ttggttatga
     2161 ttacaagtct ttaaggagac aattttctaa gaaaaatctt acagatgagg aagaaactac
     2221 gattattgaa ttttccaaag acttactcct actagctgag gagggactag tggtgagaaa
     2281 taaggaagaa atgacctatt tacagccttt gagagaagaa ttgagcctat aatttctctt
     2341 ataaagggag aattttctga aaaatcatga tataatggac gagactatag ataaaggata
     2401 gagagtaatg acattagttt atcaatcaac gcgtgatgcc aacaatacag taactgccag
     2461 ccaagcaatt ttgcaaggtt tggcgacgga cggcggtttg tttacaccgg atacttatcc
     2521 aaaggtagat ttgaactttg acaaattgaa agatgcttct taccaggaag ttgctaagct
     2581 agttttgtca gcatttttag atgactttac agttgaggag ttggactact gtatcaacaa
     2641 tgcctacgat agcaaatttg atactccagc tattgcacca ttagtgaaat tagatgggca
     2701 atacaatttg gaacttttcc atggttcaac gattgccttt aaggatatgg ccttgtctat
     2761 tttgccatac tttatgacga ctgctgctaa gaaacatggt ttggagaaca agattgttat
     2821 cttgacagcg acatctggtg acacggggaa agctgctatg gcggggtttg cgaatgtgcc
     2881 tggtactgag attatcgtct tttatccaaa ggatggtgtc agcaagattc aagagttaca
     2941 aatgaccact cagactggcg acaatactca tgttattgct attgatggta actttgacga
     3001 tgcgcaaaca aatgtgaagc acatgtttaa cgacgtggct cttcgtgaaa aattgactac
     3061 caacaagttg caattttcat cagctaactc tatgaacatt ggtcgtctgg tgccacaaat
     3121 tgtttattat gtttatgctt acgctcaatt ggttaagact ggtgaaattg tagctggtga
     3181 aaaggttaac ttcacagtac caacaggaaa ctttggaaat atcttggctg ccttttatgc
     3241 caaacaaatt ggtttgccag ttggtaaatt aatctgtgct tcaaatgaca acaatgtttt
     3301 gacagacttc tttaaaacac gtgtctatga caaaaaacgt gagtttaagg taacaaccag
     3361 cccatctatg gatatcttgg tatcttcaaa cttggagcgc ttgattttcc atcttttggg
     3421 aaataatgct gaaaagacaa ctgaacttat gaatgccttg aacacgcaag gacaatataa
     3481 gttgatagac tttgatgcag agattttgga cctctttgca gctgaatatg cgactgagga
     3541 agaaacggca gcagagatca agcgtgtttg tgagttagat tcttatatcg aggaccctca
     3601 tacagctgtt gcttcagcag tttataaaaa ataccaatcg gccactggag atgtaactaa
     3661 gacagtgatt gcttcaacag ctagtccata caagttccca gtagttgcag tagaagctgt
     3721 aactggaaaa gcaggtttaa cagactttga agccttggct caattacatg aaatctcagg
     3781 cgttgcagtg ccaccagcag ttgatgggct tgaaatagct ccaattcgtc acaagacaac
     3841 agtggcagct gctgacatgc aagcagcggt tgaggcttat ttaggacttt aagacagagg
     3901 gagcaaactc ggttgggaaa ccaactgagt ttcttttcat caggaggaga gattgtttaa
     3961 gaaaaataaa gacattctta atattgcatt gccagctatg ggtgaaaact ttttgcagat
     4021 gctaatggga atggtggaca gttatttggt tgctcattta ggattgatag ctatttcagg
     4081 ggtttcagta gctggtaata ttatcaccat ttatcaggcg attttcatcg ctctgggagc
     4141 tgctatttcc agtgttattt caaaaagcat agggcagaaa gaccagtcga agttggccta
     4201 tcatgtgact gaggcgttga agattacctt actattaagt ttccttttag gatttttgtc
     4261 catcttcgct gggaaagaga tgataggact tttggggacg gagagggatg tagctgagag
     4321 tggtggactg tatctatctt tggtaggcgg atcgattgtt ctcttaggtt taatgactag
     4381 tctaggagcc ttgattcgtg caacgcataa tccacgtctg cctctctatg ttagtttttt
     4441 atccaatgcc ttgaatattc ttttttcaag tctagctatt tttgttctgg atatggggat
     4501 agctggtgtt gcttggggga caattgtgtc tcgtttggtt ggtcttgtga ttttgtggtc
     4561 acaattaaaa ctgccttatg ggaagccaac ttttggttta gataaggaac tgttgacctt
     4621 ggctttacca gcagctggag agcgacttat gatgagggct ggagatgtag tgatcattgc
     4681 cttggtcgtt tcttttggga cggaggcagt tgctgggaat gcaatcggag aagtcttgac
     4741 tcagtttaac tatatgcctg cctttggcgt cgctacggca acggtcatgc tgttggcccg
     4801 agcagttgga gaggatgatt ggaaaagagt tgctagtttg agtaaacaaa ccttttggct
     4861 ttctctgttc ctcatgttgc ccctgtcctt tagtatatat gtcttgggtg taccattaac
     4921 tcatctctat acgactgatt ctctagcggt ggaggctagt gttctagtga cactgttttc
     4981 actacttggg acccctatga cgacaggaac agtcatctat acggcagtct ggcagggatt
     5041 aggaaatgca cgcctccctt tttatgcgac aagtatagga atgtggtgta tccgcattgg
     5101 gacaggatat ctgatgggga ttgtgcttgg ttggggcttg cctggtattt gggcagggtc
     5161 tctcttggat aatggttttc gctggttatt tctacgctat cgttaccagc gctatatgag
     5221 cttgaaagga taggaaatgc aaaaaacagc ttttatttgg gatttagacg ggactttatt
     5281 ggactcttac gaagcgattt tatcagggat tgaggagact tttgctcagt tttctattcc
     5341 ttatgataag gagaaggtga gagagtttat cttcaagtat tcggtgcaag atttgcttgt
     5401 gcgggtggca gaagatagaa atctggatgt tgaggtgcta aatcaggtgc gtgcccagag
     5461 tctggctgag aagaatgctc aggtagtttt gatgccaggt gcgcgtgagg tgctagcttg
     5521 ggcagacgaa tcaggaattc agcagtttat atatactcat aaggggaaca acgcttttac
     5581 cattctcaag gacttggggg tggaatccta ttttacagag attttaacca gtcagagtgg
     5641 ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt atcagttgaa
     5701 ttctgataat acttattata taggggatcg gactctggat gtggaatttg cccagaatag
     5761 tgggattcaa agcatcaact ttttagagtc tacttatgaa gggaatcaca ggattcaagc
     5821 gttagcagat atttcccgta tttttgagac taagtgataa aaagattgtg tcagttttgt
     5881 gacagagacc taacaaacta tttcaagtaa cctagtttgt tacaaggaat agacagttct
     5941 gttaaatagg cccgagaggg ctttttttct acattttttg tgttatgata gacaggtact
     6001 catttgaaag gaatttgaaa gaatgaagaa aagaatgtta ttagcgtcaa cagtagcctt
     6061 gtcatttgcc ccagtattgg caactcaagc agaagaagtt ctttggactg cacgtagtgt
     6121 tgagcaaatc caaaacgatt tgactaaaac ggacaacaaa acaagttata ccgtacagta
     6181 tggtgatact ttgagcacca ttgcagaagc cttgggtgta gatgtaacag tgcttgcgaa
     6241 tctgaacaaa atcactaata tggacttgat tttcccagaa actgttttga caacgactgt
     6301 caatgaagca gaagaagtaa cagaagttga aatccaaaca cctcaagcag actctagtga
     6361 agaagtgaca actgcgacag cagatttgac cactaatcaa gtgaccgttg atgatcaaac
     6421 tgttcaggtt gcagaccttt ctcaaccaat tgcagaagtt acaaagacag tgattgcttc
     6481 tgaagaagtg gcaccatcta cgggcacttc tgtcccagag gagcaaacga ccgaaacaac
     6541 tcgcccagtt gaagaagcaa ctcctcagga aacgactcca gctgagaagc aggaaacaca
     6601 agcaagccct caagctgcat cagcagtgga agtaactaca acaagttcag aagcaaaaga
     6661 agtagcatca tcaaatggag ctacagcagc agtttctact tatcaaccag aagagacgaa
     6721 aataatttca acaacttacg aggctccagc tgcgcccgat tatgctggac ttgcagtagc
     6781 aaaatctgaa aatgcaggtc ttcaaccaca aacagctgcc tttaaagaag aaattgctaa
     6841 cttgtttggc attacatcct ttagtggtta tcgtccagga gacagtggag atcacggaaa
     6901 aggtttggct atcgacttta tggtaccaga acgttcagaa ttaggggata agattgcgga
     6961 atatgctatt caaaatatgg ccagccgtgg cattagttac atcatctgga aacaacgttt
     7021 ctatgctcca ttcgatagca aatatgggcc agctaacact tggaacccaa tgccagaccg
     7081 tggtagtgtg acagaaaatc actatgatca cgttcacgtt tcaatgaatg gataaacccg
     7141 acttgataac atcattttga cgaatgagat ctagctttcg tgatggaaag cgattctcgt
     7201 tcgttttttc tttgtcatac tcttcgaaaa tctcttcaaa ccacgtcagt tttatctgaa
     7261 acttcaaagc tgtgctttga gcaacctgcg actagcttcc tagtttgctt tttgattttc
     7321 attgagtatc aatttgaatg gaaaatggaa agttatcatc ttgtaatgag ttaagcaaca
     7381 ttcttgcaat ctattttact ttatatcaca attaattagt caaatattga taaatcaata
     7441 aaaagagagg ggaagaaatg ctagagattc aagatttact gtatcaactc cgcttgtctg
     7501 agcaagcgag tacgcaattg tttgaaaaaa ggcttgggat tagtttgaca cggtatcaga
     7561 ttttactgtt tttgctggag cattctcctt gtaaccaaat ggcggttcag gagcgtttga
     7621 aaattgatca ggctgctttg acacggcatt tcaaaatttt ggaaacggaa ggtttggtgg
     7681 agcgtcatcg taatcctgaa aatcagcggg aagtgttggt agaggctgcg aagtatgcca
     7741 aggagcagtt agtggtgaat ccctctctgc aacatatcag ggttaaggaa gagatagaaa
     7801 gtatcttaac agagtttgag agaacagaac tcagccgttt attaaataaa ttggttttgg
     7861 gtattgaaaa tatagaaatt taaggagaaa tagatgtcaa ttattttaac aacgatcgtt
     7921 gctttggagc atttttacat tttttatttg gaaagtattg ccacgcaatc agatgcgact
     7981 agtcgtgtat ttaatatgga aaaggaagaa ttggctcatc cgtcagtaag ttcattgttc
     8041 aaaaatcaag gaatttataa ggctctgcta ggagtctttc tcttgtatgt catttatttc
     8101 tcacagaatt tagaaattgt gactattttt gtcttatttg tgattggtgc tgcgacttac
     8161 ggctctttaa cagcggataa aaaaattatt ttgaaacaag gtggatcagc tattttggcc
     8221 ttgattagta ttttactctt taaatacact tgaaggtcga ttctaatctc gctaatcctt
     8281 tttaatccag aataagggaa atatgttata cttgttttta agaaaaaagt ctcattgaat
     8341 tggttttgag gagttagaaa tgaaagtatt agtgacaggt tttgagccct ttggagggga
     8401 aaagggcaat ccagctttgg aggccattaa aggtttacca gctgaaatcc atggtgctga
     8461 ggtccgttgg ctagaggtgc cgacagtttt tcacaaatct gctcaagtat tggaagaaga
     8521 gatgaatcgt tatcaacctg actttgtcct ttgtattggg caagctggtg gaagaactag
     8581 tttgacacct gaacgagtga ccattaatca agacgatgca tgcatttctg ataacgaaga
     8641 taatcaaccg attgaccgtc ccattcgccc agatggtgct tcggcctact ttagtagttt
     8701 gccgattaaa gcgatggttc aagctataaa aaaagagggc ttaccggcct ctgtttccaa
     8761 tacggcaggg acttttgtct gcagccattt gatgtatcag gctctctatt tggtagaaaa
     8821 gaaatctcca tatgttaagg caggttttat gcatattcct tatatgatgg aacaggtggt
     8881 gaacagaccg actactccag ctatgagttt agtggatatt cggcgaggga tagaagcagc
     8941 aatcggcgct ataatagaac atggagatca ggaactcaag ttggtaggcg gagaaactca
     9001 ttgatagaaa aaagcttgag gagaaaaacc ttcaagcttt tggacgtttt cgggccaata
     9061 ctgctcggta aaacataatt ttagtgcatt ggatataagg taggagtgaa aaactagcaa
     9121 tgccaaaggt aatccaattg aggaagtacc aaggaagaag ctgtaaatct aggacaaagt
     9181 gctggaactt gtagcccttc ataaaggaac ggctagtttt taggattcgt cttggtggga
     9241 cctgtcctag gtctagacta taacagagaa gaaattccac ctgtgaatag gcataatact
     9301 gtggaatata gaggatattt cctacaatga tcaagatgag acttgcaaga aagtagagtc
     9361 caaagaccat gaggaaacgc tcggtttcaa ctgatgagag atctagattt ggaaactcag
     9421 gatgtagggt gacgaatttt ttggctaaaa agctactata aaagaggagg taaatcccaa
     9481 gtaaattagg gatactccat aaaaagagat agaaacgttt gagaagtagg gtcaaaaagg
     9541 tttgagaaaa gcgctcctca tcaaagagag ctaggctgtt ttttacagat ggctccgttt
     9601 tagaatcttt catgagtgtc agtgttgcat agacggaact ggtcaaaaga atagtcccga
     9661 taaaggagac tagtagagga aagaggtagg tttgaagtat ttggccaagt atgctgaaaa
     9721 atggctgttc taaaacagtc ccgtggatcc gagataaggg attaagaaaa ccagataaga
     9781 tgaccagcat actgggaagg atatagagga gaaagagacg gggggtgtca gcctgaaaat
     9841 gttttgactc ctgacgaatt gtttttaaat caatttttgg atagttcatt ctcttattat
     9901 accatagttc ttatacatag ttcgtgacag ttcctacttt ttttgataaa atcatacagt
     9961 gtgtccttgg gcacactgta tgaactggga ctgtctttcc cagcttcgga ggtaaaaaat
    10021 gtcagattca ccaatcaaat atcgtttgat taagaaagaa aaacacacag gagctcgtct
    10081 gggagaaatc atcactcccc acggtacctt tccgacacct atgtttatgc cagttgggac
    10141 acaagccact gtcaaaactc agtcacctga agaattgaag gagatgggtt cgggaattat
    10201 cctatcaaac acctatcatc tctggcttcg ccctggagat gaactcattg cacgcgctgg
    10261 tggtctccac aagttcatga attgggacca gcctatcttg acagatagtg gtggttttca
    10321 ggtttattct ttagcagata gccgtaatat cacagaagaa ggagtaacct ttaaaaatca
    10381 tctaaatggt tctaagatgt tcctatcccc agaaaaagcc atctctattc agaataatct
    10441 gggttcagac atcatgatgt cctttgatga atgtcctcag ttttatcaac cttatgacta
    10501 cgttaagaaa tcgatcgagc gtaccagccg ttgggctgag cgtggtttga aggctcaccg
    10561 tcgtccacat gaccaaggtt tgtttggaat tgtgcaaggt gcaggatttg aagaccttcg
    10621 ccgccaatca gctcatgatc ttgtcagcat ggatttctca ggctactcta tcggtggttt
    10681 ggcagtggga gaaacccatg aagagatgaa tgcggtcttg gactttacaa ctcaactgct
    10741 acctgaaaat aaacctcgtt atctgatggg tgtgggagcg ccagatagct tgatcgatgg
    10801 ggtcattcgt ggggtggata tgtttgactg tgtcttaccg actcgaattg ctcgtaacgg
    10861 gacttgtatg accagtcaag gacgtttggt tgtgaaaaat gcccagtttg ctgaggactt
    10921 tacgccactg gatcctgagt gtgactgcta cacatgtaat aactatacac gcgcttacct
    10981 tcgtcacctg ctcaaggctg atgaaacctt tggtatccgc ttgactagct accacaatct
    11041 ttacttcttg cttaacctga tgaagcaagt gcgacaagcc atcatggatg acaatctctt
    11101 ggaattccgt gagtattttg tggaaaaata tggctataat aagtcaggac gtaatttcta
    11161 aaatggaatt gatataaaaa aatcctaagt tttctcttag gatttttctt ctttttttga
    11221 tagaataaag tgtacaatga aaggaagaat aaactcgtat gcgcattaaa tggttttcct
    11281 tgattaggat tataggttta cttttggtac tcttgtatca cttctttcag acgatctttc
    11341 ctggaggatt tttcggggta gatgtctttt tcacattttc aggtttcctg attacagctc
    11401 tactcattga agaattttct aagaacaatg agattgattt gataggattt tttagaagac
    11461 gcttttatcg gattgtgcca cctgtggttt tgatggtctt ggtgaccatg ccttttactt
    11521 tcttggttcg ccaagactat gttgctggaa ttggtggcca gattgcgggc gtcttaggct
    11581 ttatgaccaa cttctatgaa ctcctaacag gtgggagtta tgaatctcag ttcattcctc
    11641 atttgtttgt tcataattgg agtttggcag ttgaggttca ctactatatt ctttggggat
    11701 tggcagtttg gttcttatcc aaacaagcta aatcaaatgg tcagttgaag gggatggtct
    11761 ttctcttatc tgctgctgcc ttcttgatca gtttcttctc catgtttatt ggtagttttc
    11821 tagtgacctc ttattcctct gtttatttct ccagtttaac tcatgtctat ccattctttt
    11881 tgggaagtat gttagcaact attgtaggtg ttcgtcagac gacttccctc gtcaagcagt
    11941 tggataaaat ctgggattta cgaaagactt tggtagtttt tggaggaggc tttggtttcc
    12001 tagttctttt gactttcttt gtcaaattca cttatctttt tgcctatctt atcggcttct
    12061 tgcttgccag tcttgcagct cttgccatga ttctggcggc gcgtgtctta catgaaaaga
    12121 cacatcatat acaggagccg aagattatca gctttttagc ggatactagc tatgcggttt
    12181 atcttttcca ttggcctttc tatatcattt tctcacagtt gacatcaaat cttcttgctg
    12241 tattactgac tctgatttgt tcttatggct ttgccagtct gtcattctat gtattggaac
    12301 cttggattgc aggcaagaac acacctattt tacaaactct tcgtcccctg ccttatattc
    12361 acgcaattct tgtagcaggt acaggaatct tgaccatcat tgtctgcacg gtgaccttgt
    12421 tggcaccaca agtgggagcg tttgagacag acttgactgt caatggcttg aagcaagctg
    12481 caacaaatat tggccagacc aaggtgatgg cagaacgggc agatgcaaac agtttgggaa
    12541 ttgctgatgg cactatgtta attggtgact cagtggcttt aagggcaaat acagcactac
    12601 agacagctct tcctggagca cagattaacg cgcaggtcag cgtaacaacc aagaccgcaa
    12661 atgaaatcat gctaaataat agccagaata aatttttacc taagacggtg gtcattgcga
    12721 ctggggtaaa taatcctgag aattacaagg atgactggga cagtatcgtg aaaaatcttc
    12781 ctaagggaca ccatatgatt ttggtgactc cttatgaggg agataagaca aaagagacct
    12841 atgccatcgt tgagaaggct gctgcctata tgagagaatt ggcagagaag acaccttaca
    12901 ttacgatagc agattggaat caagttgcga aagagcatcc agaaatttgg gctggaacag
    12961 accaggttca tttcgggagt gagagtagca ctatcgaagc aggagcaaaa ttgtatgcag
    13021 atacgattgc cacagctttg cagacagctc aagacaagcc ggttaaatca aaataacttg
    13081 tattaaaaaa gagaagagtt ggagaaatcc agctctttta aaatatctcc aaaaaatagg
    13141 tctataccat ttacaaataa aaaagaaagg tttataatgt aattgacata ataaattgta
    13201 gaatcaatct tttaaggagg ttaacattat gcctaactat attaaagcgg atcagttttt
    13261 ctacccacac ggagttcgtc gaggtggtta cttggaactt gtggacggca agtttgggaa
    13321 acatgtagag cagattcctg aaggggctga ggtgattgac tatacaggtt atagcattgc
    13381 cccaggtctt gtggatactc atattcatgg atatgcaggt gtagatgtga tggacaacaa
    13441 cattgaaggt acattgcata ctatgagtga aggacttctt agtaccggtg ttaccagttt
    13501 cttacccaca actttaacag ccacttatga gcaattgctt gcagtcactg aaaatcttgg
    13561 aaaccattat aaagaagcaa caggtgctaa gattcgtggg atttattatg aaggtccata
    13621 tttcacagaa acttttaagg gggcacaaaa tccaacttat atgagagacc cgggtgttga
    13681 ggagtttcat tcttggcaaa aagcggcaaa tggcttgctt aataaaattg cccttgcacc
    13741 agaacgtgat ggggtggaag actttgttcg tacagttacg ggcgaaggtg tgacggttgc
    13801 tcttggacat tcaaacgcga cttttgatga agccaaaaaa gcagtcgatg ctggagcgag
    13861 tgtttgggtg catgcctata atggaatgcg tgggttgact caccgtgaat tgggtatggt
    13921 tggagccatg taccaattgc cacataccta tgcagagttg atctgtgatg gtcaccacgt
    13981 agatccaaag gcttgcgaaa ttcttatcaa acaaaaagga acagaaaaca tcgctcttat
    14041 cacagactgt atgacagctg ggggattgga agacggagat tatatgttgg gagaattccc
    14101 agttgtcgtt gcaaatggaa ctgcacgcct caaatcgaca ggtaacttgg caggttctat
    14161 cctcaaactc aaagatggtt tgaagaatgt ggtcgaatgg ggaattgcga atccgcatga
    14221 agcagtcatg atggccagct tcaacccagc taaatccgtt cacatcgatg acgtctgtgg
    14281 ccaaatccgt gaaggctacg acgctgactt catcgtatta gataaagatt tggaattggt
    14341 agcaacctat ctagatggcg taaaacgtta tcaagcataa gagagaaggc agaagttaga
    14401 gactagcttc tgctttttta tacagggtac tttactggta aataaaaagt taaaaaaatc
    14461 acaaaaaaag cttgaagaaa caaagaaaaa ggcttacaat tgaactataa atattacaaa
    14521 taaaaaaacg gaggagtgct ttatgaaagc ctatacttat gttaaaccag gacttgcttc
    14581 ttttgttgat gtagacaaac cagttattcg caagccaaca gacgctattg tgcgtattgt
    14641 aaaaaccact atttgtggaa cagacctcca tattatcaaa ggggatgttc ctacttgcca
    14701 aagtggtacc attcttggcc acgaagggat tgggattgtt gaagaagttg gggaaggagt
    14761 ttccaacttc aaaaaaggcg acaaggtctt gatttcttgc gtctgtgcct gtggtaaatg
    14821 ctactactgt aaaaaaggaa tttatgccca ctgtgaagac gaagggggct ggattttcgg
    14881 tcacttgatt gatggtatgc aggctgaata tctacgtgtc cctcatgcag ataatactct
    14941 ttaccatact ccagaagact tgtcagatga agctttggtt atgctgtcag acattctgcc
    15001 tactggatat gaaattggtg tcttaaaagg gaaagtagaa cctggttgca gcgtagccat
    15061 tattggttca ggtccagttg gattggctgc tcttttaaca gcccaattct attcaccagc
    15121 taaattgatt atggtagacc tagacgataa ccgcttggaa actgccctat cattcggtgc
    15181 gactcataag gttaattctt cagaccctga aaaagccatt aaagaaattt atgatttgac
    15241 agatggtcgt ggtgtggatg tcgctatcga agctgttggt attcctgcaa catttgattt
    15301 ctgtcaaaag attatcggtg tagacggaac ggttgccaac tgtggtgtgc atggtaaacc
    15361 agttgaattc gatttagata aactttggat tcgcaacatc aatgtaacaa ctggtttggt
    15421 atctacaaat acgactccac aattgttgaa agcacttgaa agtcataaga ttgaaccaga
    15481 aaaattggta actcactatt tcaaactcag tgaaattgaa aaagcctatg aagtcttcag
    15541 taaggcagca gaccaccatg ccattaaggt cattatcgaa aacgatatct cagaagccta
    15601 agtagtaaaa atatttttgt acataagtaa atagaaattc agtcatccat cagatggctg
    15661 gattttttat caaaaaatta agaaatgagc atatttcttt ccttgtctgg cggaattggt
    15721 tataatatac ggtacaaagg aatgaatgaa tatgtatcgt gttatagaaa tgtacggaga
    15781 ttttgaaccg tggtggttct tagaaggttg ggaagaagat attgtagcaa gtagaaaatt
    15841 tgaccagtat tatgatgctc tcaaatacta caaaacttgc tggtttagat tggaacaaga
    15901 atcccctctt tataaaagta gaagcgactt gatgaccatt ttttgggacc cggaagacca
    15961 acgctggtgt gatgaatgtg atgagtattt acaacaatac cattctttgg ctcttttgca
    16021 ggatgagcag gttatcccag acgaaaaact acgctcaggc tatgaaaaac aaaccagtca
    16081 ggaaaggaat cgttcttgcc gtatgaaatt aaaatagaga aaagtaactt ttttggagtt
    16141 gcttttttta tttttctaac gctttgcgaa tagtataggt gaggaggtaa gtatggttca
    16201 agaaattgca caagaaatca ttcgttcagc tcggaaaaaa gggacgcagg atatttattt
    16261 tgtccctaag ttagacgcct atgagcttca tatgagggta ggagacgagc gctgtaaaat
    16321 tggtagctat gattttgaaa agtttgcagc cgttatcagt cactttaagt ttgtggcggg
    16381 tatgaatgtg ggagaaaaaa gacgtagtca actgggttcc tgtgattatg cctatgacca
    16441 gaagatggcg tctctacgtt tatctactgt aggcgattat cgggggcatg agagtttggt
    16501 tatccgtttg ttgcacgatg aggagcagga cttgcatttt tggtttcagg atattgaaga
    16561 attaggcaag cagtacaggc aacggggact ctatcttttt gctggtccgg ttgggagtgg
    16621 taagacgacc ttgatgcatg aattgtccaa gtcactcttt aaaggacagc aagttatgtc
    16681 catcgaagat cctgtcgaaa tcaagcagga cgacatgctt cagttgcagt tgaacgaagc
    16741 aatcggccta acctatgaaa atctaatcaa actttccttg cgtcatcgac cagatctctt
    16801 gattatcgga gaaattcgtg acagcgagac ggcgcgtgca gtggtcagag ctagtttgac
    16861 aggtgcgaca gtcttttcaa ccattcacgc caagagtatc cgaggtgttt atgagcgtct
    16921 gctggagttg ggtgtgagtg aagaagaatt ggcagttgtt ctgcaaggag tctgctacca
    16981 gagattaatc gggggaggag gaatcgttga ctttgcaagc agagattatc aagaacacca
    17041 agcagccaag tggaatgagc aaattgacca gcttcttaaa gatggacata tcacaagtct
    17101 tcaggctgag acggaaaaaa ttagctacag ctaagcaaaa aaatatcatc accctattta
    17161 acaatctctt ttctagcggt tttcatctgg tggagactat ctccttttta gataggagtg
    17221 ctttgttgga caagcagtgt gtgatccaga tgcgtgcggg cttgtctcag gggaaatcat
    17281 tctcagaaat gatggaaagt ttgggatgtt caagtaccat tgtcactcag ttatctctag
    17341 ctgaagttca tggcaatctt cacctgagtt tgggaaagat agaagaatat ctagacaatc
    17401 tggccaaggt caagaaaaag ttaattgaag tagcgaccta tcctttgatt ttgctgggtt
    17461 ttcttctctt aattatgctg gggctacgga attacctgct cccacaactg gatagtagca
    17521 atattgccac ccgaattatc ggtaatctgc cacaaatttt tctaggcatg gtagggcttg
    17581 tttccgtgct tgccctttta gcactaactt tttataaaag aagttctaag atgagtgtct
    17641 tttctatctt agcacgcctt ccctttattg gaatctttgt gcagacctac ttgacagcct
    17701 attatgcacg tgaatggggg aatatgattt cacagggaat ggagctgacg cagatttttc
    17761 aaatgatgca ggaacaaggt tcccagctct ttaaagaaat cggtcaagat ctggctcaaa
    17821 ccctgaaaaa tggccgtgaa ttttctcaga cgataggaac ctatcctttc tttaggaagg
    17881 aattgagtct catcatagag tatggggaag ttaagtccaa gctgggtagt gagttggaaa
    17941 tctatgctga aaaaacttgg gaagcctttt ttacccgagt caaccgcacc atgaatttgg
    18001 tgcagccact ggtttttatc tttgtggcac tgattatcgt tttactttat gcggcaatgc
    18061 tcatgcccat gtatcaaaat atggaggtaa atttttaaaa tgaaaaaaat gatgacattc
    18121 ttgaaaaaag ctaaggttaa agcttttaca ttggtggaga tgttagtagt cttgctgatt
    18181 atcagcgtgc ttttcttgct ctttgtacct aatctgacca agcaaaaaga agcagtcaat
    18241 gacaaaggaa aagcagctgt tgttaaggtg gtggaaagcc aggcagaact ttatagcttg
    18301 gataaaaatg aagatgctac tctgagcaag ttacaaggag atgggcgcat cacggaagaa
    18361 caggttaaag cttataatga atactatact aaaaatggag gggcaaatcg taaagtcaat
    18421 gattaaggcc tttaccatgc tggaaagtct cttggttttg ggacttgtga gtatccttgc
    18481 cttgggctta tccagctctg tccagtccac ttttgcagca gtagaggagc agattttctt
    18541 tatggagttt gaagaactct atcgggaaac ccaaaaacgc agtgtagcca gtcagcaaaa
    18601 gactagtcta aatttagatg ggcagacgct tagcaatggc agtcaaaagt tgacagttcc
    18661 taaaggaatt caggcaccat caggccaaag tattacattt gaccgagctg ggggcaattc
    18721 gtccctggct aaggttgaat ttcagaccag taaaggagcg attcgctatc aattatatct
    18781 aggaaatgga aaaattaaac gcattaagga aacaaaaaat tagggcagtg attttactgg
    18841 aagcagtagt cgctctagct atctttgcca gcattgcgac cctccttttg ggacaaattc
    18901 aaaaaaatag gcaagaggaa gcaaaaatct tgcaaaagga agaagtcttg agggtagcta
    18961 agatggccct gcagacgggg caaaatcagg taagcatcaa cggagttgag attcaggtat
    19021 tttctagtga aaaaggattg gaggtctacc atggttcaga acagttgttg gcaatcaaag
    19081 agccataagg tcaaggcttt taccttgtta gaatccctgc ttgccctcat tgtcatcagt
    19141 gggggattac tcctttttca aactatgagt cagctcctca tttcagaagt tcgctaccag
    19201 caacaaagcg agcaaaagga gtggctcttg tttgtggacc aacttgaggc agaattagac
    19261 cgttcgcagt tcgaaaaagt agaaggcaat cgcctataca tgaagcaaga tggcaaggac
    19321 atcgccatcg gtaagtcaaa gtcagacgat tttcgtaaaa ccgatgctag tggacggggg
    19381 tatcagccta tggtttatgg cctcaaatca gctcagatta cagaggacaa tcaactggtt
    19441 cattttcgtt tccagtttca aaaaggctta gaaagggagt tcatctatcg tgtggaaaaa
    19501 gaagaaagtt aaggcaggtg ttctcctcta cgcagtcacc atagcagcca tctttagtct
    19561 tttgttgcaa ttttatttga accgacaaat cgcccactat caagactatg ctttaaataa
    19621 agaaaaattg gttgcttttg ccatggctaa acgaaccaag gataaagccg agcaagaaag
    19681 tggggaacag gtttttaatc taggtcaggt aagctatcaa aacaagaaaa caagcttggt
    19741 gacgactgtt cgtacgtcta agagccaata tgagtttctg tttccttcag tcaaaatcaa
    19801 agaagagaaa agagataaaa aggaagaggt agcgaccgat tcaagcgaaa aagcggagaa
    19861 aaaaaatcag aagagaagcc tgaaaagaaa gagaattcct agtcaattca actataatgc
    19921 gttgaatcca gaatagtcca ctgtagtttc tagaaaattg ctggaaatgg atgttaagct
    19981 ccaattcatt tgtttatatc ttatttcagt ttactatact ttgtgctaaa ctaaagatat
    20041 gaaacatgat tttaaccaca aagcagaaac tttcgactcc cctaaaaata tcttcctcgc
    20101 aaacttggta tgtcaagcag tcgagaaaca gattgatatt ttatcagaca aagtaatttt
    20161 agatttcggt ggtggcacgg gtctattagc cttgccccta gccaagcagg ctaagtcagt
    20221 tactcttgta gacatttctg agaaaatgtt ggagcaagct cgtttgaaag tggagcagca
    20281 agcaatcaag aatatccagt ttttggagca agatttaccg aaaaatccct tggagaaaga
    20341 gtttgattgc cttgctgtta gtcgggttct tcatcatatg cctgatttgg atgcggctct
    20401 ctcactgttt catcaacatt tgaaggaaga tgggaaactc atcattgctg attttaccaa
    20461 gacagaagct aatcatcatg gatttgattt agctgaactg gaaaacaagc taattgagca
    20521 tggtttttca tctgtgcata gtcagattct ctatagcgct gaagacctgt ttcaaggaaa
    20581 tcactcagaa ttctttttaa tagtagccca aaaatcactc gcctagtcag ggagtgattt
    20641 ttctataagg atggaaaaaa gaagggaaat ttgataagat aggaatatgg attttgaaaa
    20701 aattgaacaa gcttatacct atttactaga gaatgtccaa gttatccaaa gtgatttggc
    20761 gaccaacttt tatgacgcct tggtggagca aaacagcatc tatctggatg gtgaaactga
    20821 gctaaaccag gtcaaagaca acaatcaggc ccttaagcgt ttagcactac gcaaagaaga
    20881 atggctcaag acctaccagt ttctcttgat gaaggctggg caaacagaac ccttgcaggc
    20941 caatcaccag tttacaccag atgccattgc tttgcttttg gtgtttattg tggaagagtt
    21001 gtttaaagag gaggaaatta ctatcctcga aatgggttct gggatgggaa ttctaggcgc
    21061 tactttcttg acctcgcttg ataaaaaggt ggattacttg ggaatggaaa tggatgattt
    21121 gctgattgat ctggcagcta gcatggcaga tgtaattggt ttgcaggctg gctttgtcca
    21181 aggagatgcc gttcgtccac aaatgctcaa agaaagcgat gtggtcatca gtgacttgcc
    21241 tgttggctat tatcctgatg atgccgttgc gtcgcgccat caagttgctt ctagccaaga
    21301 acatacttac gcccatcact tgctcatgga acaagggctt aagtacctca agtcagacgg
    21361 atacgctatt tttctagctc cgagtaattt gttgaccagt cctcaaagtg atttgttaaa
    21421 agaatggctg aaagaagagg cgagtctggt tgctatgatt agtctgcctg aaaatctctt
    21481 tgctaatgcc aaacaatcta agactatttt tatcttacag aagaaaaatg aaatagcagt
    21541 agagcctttt gtttatccac ttgctagctt gcaagatgca agtgttttaa tgaaatttaa
    21601 agaaaatttt caaaaatgga ctcaaggtac tgaaatataa aatagatttt gttataatag
    21661 ttgaaaacgc ttaaaaaggg gtatcatgtt atgacaaaaa caattgcaat caatgcagga
    21721 agttcaagtt tgaaatggca attatactta atgccagaag aaaaagtgtt agccaaaggt
    21781 ttgattgaac gtatcggttt gaaagattca atttcaactg taaaatttga cggccgttct
    21841 gaacaacaaa ttttggatat tgaaaatcat acacaagccg ttaaaatttt attggatgac
    21901 ttgattcgtt tcgatattat caaggcttat gacgagatta caggtgttgg acatcgtgtt
    21961 gttgctggtg gagaatattt caaagaatca acagttgttg agggagatgt tttagaaaaa
    22021 gttgaagagt tgagtttgtt ggctcctcta cacaacccgg ccaatgcagc aggtgttcgt
    22081 gccttcaagg aattgttgcc agacattacc agtgtagttg tttttgatac ttccttccac
    22141 acaagtatgc cagagaaagc ttatcgctac cctctaccaa caaaatatta cacagaaaac
    22201 aaggttcgta aatacggtgc tcatggtaca agtcaccagt ttgtagcagg agaagctgca
    22261 aaactcttgg gacgtccatt agaagacttg aagttaatta cctgtcatat tggtaacgga
    22321 ggctcaatta cagctgtgaa agccggcaaa tctgtagaca cttctatggg gttcactcct
    22381 cttggtggta ttatgatggg aacgcgtaca ggggatattg atccagctat cattccttat
    22441 ttaatgcaat atacagagga ttttaacaca ccagaagata tcagtcgtgt tcttaaccgt
    22501 gaatcaggtc ttttgggagt ttctgctaat tctagcgata tgcgcgatat agaagcagct
    22561 gtagcagaag ggaatcacga ggctagcttg gcttatgaaa tgtatgttga ccgtatccaa
    22621 aaacatatcg gtcagtactt tgcagtgcta aatggagcag atgccattgt tttcacagca
    22681 ggtgtcggtg aaaatgcaga gaatttccgt cgtgatgtaa tctcagggat ttcgtggttt
    22741 ggttgtgatg ttgatgatga aaagaatgtc tttggcgtta caggagacat ctcaacagag
    22801 gcagctaaaa tccgtgtctt ggttattcca acagatgaag aattagtcat tgcccgtgac
    22861 gttgaacgct tgaaaaaata agtgaaacta aaaaaatatt caatacaagg agttgggaaa
    22921 gttatttttc cagcttcttt ttctgatgaa attgtccaaa accttgctat gattggcttt
    22981 tttgaaaaat atggtataat agtagtaatt taatagatgg agttgagttt tgaagaaaaa
    23041 ctttcgtgta aaaagagaga aagattttaa ggcgattttc aaggagggga caagttttgc
    23101 taatcgcaaa tttgtggtct accaattaga aaaccagaaa aaccattttc gagtaggtct
    23161 atcagttagc aaaaaactgg ggaatgccgt cactagaaat caaattaagc gacggattcg
    23221 gcatattatc cagaatgcaa aagggagtct ggtagaagat gtcgactttg ttgtcattgc
    23281 tcgaaaagga gtcgaaacct tgggatacgc agagatggag aaaaatctac tccatgtatt
    23341 aaaattatca aagatttacc gggaaggaaa tgggagtgaa aaagaaacta aagttgacta
    23401 gtttgctagg actgtctctg ttaatcatga cagcctgtgc gactaatggg gtaactagcg
    23461 atattacagc cgaatcggct gatttttgga gtaaattggt ttacttcttt gcgaaaatca
    23521 ttcgcttttt atcgtttgat attagtatcg gagtggggat tattctcttt acggtcttga
    23581 ttcgtacagt cctcttgcca gtctttcagg tgcaaatggt ggcttctagg aaaatgcagg
    23641 aagctcagcc acgcattaag gcgcttcgag aacaatatcc aggtcgagat atggaaagca
    23701 gaaccaaact agagcaggaa atgcgtaaag tatttaaaga aatgggtgtc agacagtcag
    23761 attctctttg gccgattttg attcagatgc cggttatttt ggccttgttc caagccctat
    23821 caagagttga ctttttaaag acaggtcatt tcttatggat taaccttggt agtgtggata
    23881 caacccttgt tcttccgatt ttagcagcag tattcacctt tttaagtact tggttgtcca
    23941 acaaagcttt gtctgagcga aatggcgcta cgactgcgat gatgtatggg attccagtct
    24001 tgatttttat ctttgcagtt tatgcgccag gtggagtcgc cctatactgg acagtgtcta
    24061 atgcttatca agtcttgcaa acctatttct tgaataatcc attcaagatt atcgcagagc
    24121 gcgaggccgt agtacaggca caaaaagatt tggaaaatag aaaaagaaaa gccaagaaaa
    24181 aggctcagaa aacgaaataa ataaggagga atctggtaat ggtagtattt acaggttcaa
    24241 ctgttgaaga agcaatccag aaaggattga aagaattaga tattccaaga atgaaggctc
    24301 atatcaaagt catttctagg gagaaaaaag gctttcttgg tctatttggt aaaaaaccag
    24361 cccaagtgga tattgaagcg attagtgaaa cgactgttgt caaagcaaat caacaggtag
    24421 taaaaggcgt tccgaaaaaa atcaatgatt tgaacgagcc tgtgaagacg gttagtgaag
    24481 aaaccgttga ccttggtcat gtggttgatg ctattaaaaa aatagaggaa gaaggtcaag
    24541 gtatttctga tgaagtcaag gctgaaatct taaaacatga aagacatgcc agcactatct
    24601 tagaagaaac tggtcacatt gagattttaa atgaacttca aatcgaggaa gcgatgaggg
    24661 aagaagcagg cgctgatgac cttgaaactg agcaagatca aactgaaaat caagacttga
    24721 aagagatggg cttgaaggtc gagcaaagtt atgatattgc ccaggtggct acggatgtga
    24781 ctgcctatgt tcaagcgatt gtggatgaca tggatgttga agctacactt tcaaatgatt
    24841 ataaccgtcg tagcatcaat ctacaaattg acaccaacga accaggtcgt attatcggct
    24901 accatggtaa agtcttgaag gccttgcaac tgttggctca aaattatctt tacaaccgct
    24961 attccaaaac cttctacgtt acaatcaatg tcaatgatta tgtcgaacac cgtgcagaag
    25021 tcttgcagac ctatgcgcaa aaattggcga ttcgtgtttt agaagaagga cgtagtcatc
    25081 aaacagatcc aatgtcgaat agtgaacgca agattatcca ccgtattatt tcacgtatgg
    25141 atggcgtgac cagttactct gaaggtgatg agccaaatcg ctatgtcgtt gtagatacag
    25201 aataagtaaa atcagggttg acctgatttt ttgctagcta gaggaggtta aactgatgtt
    25261 gaataagata agagactatt tagactttgc tggtttgcag taccgtaatc ctgataaagc
    25321 gggagcagag cgagagaaga tgctggcatt ccgccacaaa ggacaagagg cccgaaaggc
    25381 ttttacagaa ctggccaaag cctttcaagc aagccatcca gaatggcaac tccaacagac
    25441 tagtcagtgg atgaatcagg cccagcgttt gagaccacat ttttgggttt atctacagag
    25501 agacggacaa gtgacagaac ctatgatggc cttacgtttg tatgggacat ctactgactt
    25561 tggaatttct ttggaagtca gtttcatcga acgtaagaag gatgagcaaa cactgggcaa
    25621 gcaggccaaa gttttagaca ttccaaccgt taaagggatt tattatctaa cctactctaa
    25681 tggtcaaagt caacggtggg aggcgaatga agaaaagcgt cgtactttac gcgagaaggt
    25741 gagaagtcaa gaagttcgaa aagttttagt gaaggtagat gttcctatga cagaaaattc
    25801 gtctgaagaa gaaatcgtag aaggcttatt gaagtcttat tctaaaattc ttccctatta
    25861 tctagctacg agaaaataag ataatttgta aaacatcata aatcatacag tccaagagtg
    25921 aacagtccgc tgtgtaattc ttggtctttt tgtttgcgct ttcgcattat ataataaact
    25981 tacaaaaaca attcaaaagg agaacaatta tggaagtcgt ttcaagtgtt ctaaattggt
    26041 tttctagcaa tattttgcag aatcccgcat ttttcgtagg tttattggtg ttgataggat
    26101 atgcactttt gaaaaaacct gcccatgacg ttttttcagg gtttgttaaa gcaacagtag
    26161 ggtatatgtt gcttaacgtg ggtgctggtg gtttggttac aacctttcgt ccaatcttag
    26221 cagctcttaa ctacaaattc caaattggtg cagcggttat cgacccttac tttggacttg
    26281 ctgcagcaaa caacaaaatt gcagcagagt ttccagattt tgttggaact gcaactacag
    26341 ctctattgat tggttttgga ataaatatct tgctcgtagc tcttcgaaag attacgaagg
    26401 taagaaccct ctttattact ggtcacatca tggtacaaca agctgcaaca gtatctctta
    26461 tggttctatt cttagtacca caattgcgca atgcttacgg tacagcagcg attggtatca
    26521 tctgtggact ttactgggca gttagttcaa atatgactgt tgaggcaact caacgcttga
    26581 ctggtggtgg cggatttgcg attggtcacc aacagcaatt tgcaatctgg tttgtagata
    26641 aagtagcagg acgctttggt aagaaagaag aaagtttaga caatcttaaa ttacctaagt
    26701 tcctctcaat cttccacgat acagttgttg catctgctac cttgatgctc gtattcttcg
    26761 gggccattct tttaatcttg ggtccagaca ttatgtctaa taaagaagtc atcacttcag
    26821 gaactctatt caatcctgct aaacaagatt tctttatgta cattatccaa acagccttta
    26881 ccttctcagt ttacttgttc gttttgatgc aaggtgtccg aatgttcgta tctgagttga
    26941 caaacgcctt ccaaggtatt tcaaacaaat tgttgccagg ttcattccca gcggttgacg
    27001 ttgcagcttc ttatggattt ggttctccaa atgctgtctt gtcaggattt acctttggtt
    27061 tgattggtca attgattaca attgtcttgc tcatcgtctt taaaaatccg attcttatta
    27121 ttacaggatt tgtaccagtg ttctttgaca atgcagccat tgcggtctac gctgataaac
    27181 gcggcggatg gaaagcggct gttatccttt cctttatatc aggtgtcctt caagttgctc
    27241 taggagctct ttgtgtggcc cttctcgatt tggcatctta tggtggctac catggaaata
    27301 tcgactttga attcccatgg cttggatttg gatatatctt caaatacctt ggtattgttg
    27361 gttatgtact tgtgtgtctc ttcttgcttg ttattcctca acttcaattt gccaaagcaa
    27421 aagataaaga gaaatattac aacggtgaag ttcaagaaga agcttagtat ctagaaaagg
    27481 agaaataaaa tggttaaagt attagcagcg tgcggaaatg gaatgggttc atcaatggtt
    27541 atcaagatga aggttgaaaa tgctctccgt aagcttaatc aaacagattt tacagtcaat
    27601 tcatgcagtg tcggtgaagc taaaggttta gcagtaggat atgacatcgt aatcgcttct
    27661 cttcatttga ttcaagaatt ggaagggcga actaatggga agttaattgg acttgataac
    27721 ttgatggatg ataaagaaat caccgaaaaa ctcagtcaag cactacagta aaaggttgga
    27781 gggggctgga cagaaactga gagttatcgt ttctgtcctt ctccctcttt aaataaagga
    27841 ggcagatatg aatttaaaac aagctttaat tgacaatgac tcgatccgac taggtttaga
    27901 ggctaacaat tggaaagaag cagtcaaggt agcagtagat cccttaattg aaagtggggc
    27961 aattttgcca gagtattacg atgctatcat tgaatcgact gaagagtatg ggccttacta
    28021 tatcttgatg ccaggtatgg ctatgcccca cgctagacct gaagctggtg tgcaaagtga
    28081 tgccttttca ttgattacct tacaaaatcc tgttgtattt tcagatggga aagaggtatc
    28141 tgttttgttg gcactagcag caacaagctc aaaaattcac acaagtgtag ccattccaca
    28201 aattattgcc ctgtttgaat tagaagattc tattgcacgt ttacaggctt gccagactaa
    28261 agaagatgtc ttggctatga ttgaagaatc taaggatagc ccttatctcg aaggattgga
    28321 tttggaaagt tagaaagagg aataaagaaa tgacaaaaag aatacctaat ttacaagttg
    28381 cattagacca ttcagacttg caaggagcga ttaaagcagc tgtttctgtt ggtcaggaag
    28441 tagatattat cgaagctgga actgtttgct tgcttcaagt tggaagtgaa ctggctgaag
    28501 tcttgcgtag ccttttccca gataagatta ttgtggcaga cacaaaatgt gctgatgctg
    28561 gtggaacagt tgctaaaaat aatgcggttc gtggagcaga ctggatgact tgtatctgtt
    28621 gtgcaaccat ccctactatg gaagcagctc taaaggctat caagactgaa cgaggagaac
    28681 gaggcgaaat ccagatcgag ctttatggcg attggacttt tgaacaagct cagctttggc
    28741 tagatgcagg tatctcacaa gctatttatc accaatctcg tgatgctctt cttgctggtg
    28801 aaacttgggg tgaaaaagac cttaataagg ttaaaaaact cattgacatg ggcttccgtg
    28861 tatctgtaac aggtggtcta gatgtagata ctctcaaact ctttgaaggt gttgatgtct
    28921 ttacctttat cgcaggtcgt ggaattacag aggctgcgga tccagcagga gcagcgcgtg
    28981 ccttcaagga tgaaatcaaa cgaatttggg ggtaaatcat ggtacgtcca attggaattt
    29041 atgaaaaggc aaccccaaca cactttactt ggctagaacg tttaaatttt gccaaggagt
    29101 taggctttga ttttgtcgag atgtctattg acgaacgtga cgagcgttta gcaagacttg
    29161 actggagtaa ggaagaacgc ttggaagttg tcaaagcaat ctatgaaact ggtgttcgta
    29221 ttccttctat ctgtttttca ggccatcgtc gctacccatt gggttcaaaa gatccagttc
    29281 tagaggaaaa atctctagaa ctcatgaaaa aatgtatcga attagctcaa gacttgggag
    29341 ttcgtacgat tcaattagct ggttacgatg tttactatga ggaaaagtca ccccagacac
    29401 gccaacgttt tatcaaaaat ttgagaaaag cctgtgactg ggctgaagaa gctcaggtgg
    29461 tacttgctat tgaaattatg gatgatcctt tcatcaatag catcgaaaaa tatttggcta
    29521 tagaaaaaga gattgactct cccttcctct ttgtatatcc agatattggt aatgtgtctg
    29581 catggcataa tgatatctat agtgagtttt atcttggtca tcatgccatc gcagctctcc
    29641 atctcaagga tacttatgca gtgacagaaa gttcaaaggg ccagttccga gatgtacctt
    29701 tcgggcaagg ttgtgtcaaa tgggaagaag ctttcgatat tttaaaggaa accaattata
    29761 atggaccttt cctaatcgaa atgtggtctg aaaattgtga aacagtagaa gaaacacgcg
    29821 cagccattca agaggcgcaa gcttttctct atccactcat taagaaagca ggtttgatgt
    29881 aagatgaatc aagtaatcaa tgctatgcgt aaacgagtct gtgatgccaa tcaatcattg
    29941 ccaaaacatg gacttgtcaa atttacctgg gggaatgtat ctgaagttaa tcgcgaactc
    30001 ggtgtcattg ttatcaaacc atcaggcgtg gattatgacg aattgacacc tgaaaacatg
    30061 gtagtgactg atctagatgg taagatccta gaaggggatt taagaccatc ttccgacctc
    30121 ccaactcatg tgcaattata taaggcttgg tcagaaattg gtagtgtggt tcacacccat
    30181 tcgacagaag ctgttggttg ggctcaggca ggtcgtgata ttcctttcta cggaacaacc
    30241 catgcagatt atttctacgg ttcaatccct tgcgcccgta gtttgaccaa ggacgaagta
    30301 gaagtggcct atgaaaaaga tactggcctg gttatcgtag aagagtttga acatcgcgga
    30361 cttaacccgg ttgaagtacc aggaattgtt gtacgcaatc acggtccatt cacctggggc
    30421 aaaaatccag agaatgctgt ttatcactct gtcgtactag aggaagtatc aaagatgaat
    30481 cgctttacag aacaaatcaa tccaagagtt ggacctgctc cccagtacat actagaaaaa
    30541 cactaccaac gtaaacatgg accaaatgct tattatggtc aaaagtaaga acgatgaagg
    30601 aggagaaaaa gataaattta gctcctcttt ttacatttga tttttattga gagtaaagtt
    30661 ggaattgaag taattttaaa agatttttta gaaatagcgc ttgatatata tatggtaaaa
    30721 taaaaagaat tgctgtgata tcaatagatt tgggggattt tttaatatgg tactggataa
    30781 ggcaagttgt gatttgcttc aatatttgat ggatcaagaa acgtccaaaa cgattatggc
    30841 gatttcgaaa gatttgaaag agtcaagaag gaaaatttat tatcacattg acaaaatcaa
    30901 tgctgctctg gttgacgagg cgcttcacat cattagtatt ccacgaattg gtattcactt
    30961 aacggaagag cagagagatg cttgttgtaa actattatcg gaagtagatt cgtacgatta
    31021 tatcatgagt gcgcatgaac gtatgatgat aatgttacta tggataggta tttctaaaga
    31081 acgtattacg attgaaaaat tgatagagtt aacagaggta tctaggaata ctgttctcaa
    31141 tgatttgaat agtattcgtt atcaactaac tttggaacaa tatcaggtga tcttgcaagt
    31201 gagcaagtca cagggatacc accttcatgc ccaccctctt aataaaattc agtatcttca
    31261 atcgcttcta tatcatattt ttatggaaga aaatgccact tttgtatcta ttttagaaga
    31321 taagatgaaa gagaggttag atgatgagtg tttgctttct gttgaaatga accaattttt
    31381 taaggaacag gttcctttag ttgaacaaga tttagggaag aaaataaacc atcatgaaat
    31441 aacttttatg ttgcaggttc taccttattt gctgttaagc tgtcataatg ttgaacagta
    31501 tcaagaaaga catcaggata tagagaaaga attttctttg ataagaaaaa gaatagagta
    31561 tcaggtgtct aagaaattag gagaacggtt gtttcaaaag tttgaaattt ctttgtcagg
    31621 acttgaagtt tctcttgtag ctgttctcct cctctcctat cgtaaggatt tggatattca
    31681 tgcagaaagt gatgattttc ggcaattaaa acttgcttta gaagaattta tctggtattt
    31741 tgaatcacaa atccgaatgg agattgagaa caaggatgat ttgttacgaa atttgatgat
    31801 ccactgtaaa gccttgttat ttagaaagac ttacggtatt ttttctaaaa atcctctaac
    31861 aaaacaaatt cgatccaagt atggagaatt atttttagtc actagaaaat ctgcggaaat
    31921 tttagaagga gcatggttta ttcggctaac agacgatgat attgcctatt tgacgattca
    31981 tattggagga tttttaaagt atacaccatc gtctcaaaaa aatatgaaaa aagtttatct
    32041 cgtttgtgat gaaggtgttg cggtttcgag acttttgctg aaacaatgca aactttattt
    32101 tccaaatgag caaattgaca ctgtatttac aacagaacaa tttaagagtg tggaagatat
    32161 tgcacaagtt gatgtagtga ttactactaa tgatgatttg gatagcagat ttccgatttt
    32221 aagggttaat cctatccttg aagcagaaga tattttgaaa atgctagact atcttaaaca
    32281 caatatattt cgtaataaga gcaaaagttt cagtgaaaat ctttctagtc ttatttcgtc
    32341 ttatattgta gacagcaagt tggctagtaa gttccaagaa gaggttcaaa cacttataaa
    32401 taaagaaata gtagttcaag cttttttgga agatatttga aggacagtcc aatgatgaac
    32461 acaaacctgt gtttttcttg gtctttttta gtgttttgaa gggtggtata ctaatctcaa
    32521 agataacaat tatatccaaa ggaggcaaca tatgccaaac gtcaaagaaa ttacaagaga
    32581 gtcatggatt ttagccactt tcccagagtg gggaacatgg ttgaacgaag aaatcgaaga
    32641 agaagtcgta cctgaaggca actttgccat gtggtggcta ggcaactgtg gtacttggat
    32701 taagacacca gctggtgcta acgttgtcat ggacctttgg tcaaaccgtg gaaaatcaac
    32761 caaaaaagtg aaagatatgg ttcgtgggca ccaaatggca aatatggcag gtgttcgtaa
    32821 gttgcaacca aacttgcgtg ttcagccaat ggttatcgat ccatttgcta tcaacgaact
    32881 agactattac ttagtttcac acttccacag tgatcatatc gacccataca cagctgcagc
    32941 aattctcaac aatcctaagt tagagcatgt taagtttatc ggtccttacc actgtggacg
    33001 aatctgggaa ggatggggtg ttccaaaaga acgtatcatc gttgttaaac caggtgacac
    33061 tatcgaatta aaagatatga agattcatgc agtagaatca tttgaccgta cttgcttggt
    33121 aactctccca gtgaacggtg ctgatgagac aggcggtgaa cttgctggct tggctgttac
    33181 agatgaagaa atggctcaaa aggctgttaa ctatatcttt gaaacaccag gtggaaccat
    33241 ctatcatggt gcagattctc acttctcaaa ctattttgca aaacatggta aagactttaa
    33301 aattgatgtt gctttgaata actatggtga aaatccggta ggtatccaag acaaaatgac
    33361 atctatcgat cttcttcgta tggcagaaaa tctgcgtacc aaagtcatta tcccagttca
    33421 ctatgatatc tggtctaact tcatggcttc tactaatgag attctagaac tttggaaaat
    33481 gcgaaaagat cgcttgcaat acgatttcca tccatttatc tgggaagttg gcggtaagta
    33541 cacttatcct caagatcaac acttagtaga ataccatcat ccacgtggtt ttgatgattg
    33601 ttttgaacaa gactctaaca ttcaatttaa agctttgcta taatataaaa atattctttg
    33661 gaggttatcc gtatgttgca tgatacggat aattttcata taatagtaaa atagaatgtg
    33721 tgattcaata atcacctcaa atagaaagga aattctatgt caaatctatc tgttaatgca
    33781 attcgttttc taggtattga cgccattaat aaagccaact caggtcatcc aggtgtggtt
    33841 atgggagcgg ctccgatggc ttacagcctc tttacaaaac aacttcatat caatccagct
    33901 caaccaaatt ggattaaccg cgaccgcttt attctttcag caggtcatgg ttcaatgctc
    33961 ctttatgctc ttcttcacct ttctggtttt gaagatgtca gcatggatga gattaagagt
    34021 ttccgtcaat ggggttcaaa aacaccaggt cacccagaat ttggtcatac ggcagggatt
    34081 gatgctacga caggtcctct agggcaaggg atttcaactg ctactggttt tgcccaagca
    34141 gaacgtttct tggcagccaa atataaccgt gaaggctaca atatctttga ccactatact
    34201 tacgttatct gtggagacgg agacttgatg gaaggtgtct caagcgaggc agcttcatac
    34261 gcaggtttgc aaaaacttga taagttggtt gttctttatg attcaaatga tatcaacttg
    34321 gatggtgaga caaaggattc ctttacagaa agtgttcgtg accgttacaa tgcctacggt
    34381 tggcatactg ccttggttga aaatggaaca gacttggaag ccatccatgc tgctatcgaa
    34441 acagcaaaag cttcaggcaa gccatcttta attgaagtga agacggttat tggatatggt
    34501 tctccaaaca aacaaggaac taatgctgta cacggcgccc ctcttggagc agatgaaacc
    34561 gcatcaactc gccaagccct cggttgggac tacgaaccat ttgaaattcc agaacaagta
    34621 tatgctgatt tcaaagaaca tgttgcagac cgtggcgcat cagcttatca agcttggact
    34681 aaattagttg cagattataa agaagctcat ccagaactgg ctgcagaagt agaagccatc
    34741 atcgacggac gtgatccagt cgaagtgact ccagcagact tcccagcttt agaaaatggt
    34801 ttttctcaag caactcgtaa ttcaagtcaa gatgccttga atgttgtggc agctaaatta
    34861 ccaaccttcc taggtggatc agctgacctt gctcactcaa acatgactta tatcaagacg
    34921 gatggacttc aagacgatac caatcgcttg aatcgtaaca ttcagtttgg tgttcgtgaa
    34981 tttgcaatgg gaacaatctt gaacgggatg gcccttcatg gtggacttcg tgtatacggt
    35041 ggaactttct tcgtcttctc tgactatgtg aaggcagctg tccgcttgtc agccttacaa
    35101 ggacttcctg tgacttatgt ctttacccat gattcaatcg cagttgggga agatggtccg
    35161 actcacgaac cagttgagca tttagcaggt cttcgtgcta tgccaaatct aaatgttttc
    35221 cgtccagcag atgcgcgtga aacgcaagca gcttggtacc ttgcagtgac aagtgagaaa
    35281 acaccaactg cccttgtctt gacacgtcaa aacttgactg ttgaagatgg aacagacttc
    35341 gacaaggttg ctaaaggtgc ttatgttgta tatgaaaatg cagccgactt tgataccatc
    35401 ttgattgcga caggttcaga ggttaatctt gctgtctcag ctgctaaaga attggctagt
    35461 caaggcgaaa aaatccgcgt agtcagcatg ccatctacag atgtctttga taaacaagat
    35521 gcagcttaca aggaagagat tcttccaaat gcagtccgcc gtcgtgttgc agtcgaaatg
    35581 ggtgcaagtc aaaactggta caaatatgtt ggtctcgatg gtgccgttct aggtattgat
    35641 accttcggag cctctgcccc agcaccaaaa gtattggcag aatatggctt tactgtagaa
    35701 aatcttgtaa aaattgttcg aaacttgaaa taatcctaaa aatcagggcg taagctctgg
    35761 tttttcttac cagaaaagta aggtacaatc ttgtaaaagt agctgaaatt tgatatagta
    35821 gtcctatgta aaagacaaag gagaaaacaa tgaatccaaa tattactttt ttaatcatgc
    35881 ttgtaggtat gatggccttg atgttcttta tgcaacgttc tcaaaagaaa caagctcaaa
    35941 agcgtatgga aagcttaaat aaactacaaa aaggctatga agtgattaca atcggtggac
    36001 tttatggaac agtcgatgaa gtagatacgg agaagggaac aatagttctt gacgtagatg
    36061 gagtttactt gacttttgaa ctagctgcta tcaagacagt attaccgctg aaagaaacag
    36121 cttcactaga aggcgcaatt gaaaaataag acgggatcac gaactcccgt ttttctataa
    36181 aagaaaggaa atgggatgaa aaaattagtc tttgtctgtc tgggaaatat ttgccgtagc
    36241 cctatggccg agtttgttat gaaatcaatg acagataact acgaaatcca aagtcgagca
    36301 acttcctctt gggaacatgg caatccgatt cataagggga ctcagggaat ttttcaagag
    36361 tatgagattc cttatgacaa gaacaagaca tcgcttcaga ttagtaagga agattttgaa
    36421 gcctttgatt atattatcgg aatggacgct tcaaatattt ctgacttatg tcagatgtgt
    36481 ccagtagact gtcaagataa gatttactca ttttcatctg aaagtgttcc agacccttgg
    36541 tatacaggag attttgaaga aacctatcga cgtgttcaag agggctgtca agcttggtta
    36601 gaacgtttag aaaaggagag ttaaggtgga aaatcttacg aatttttacg aaaagtatcg
    36661 tgtctatctg actcgtccac gtttagagct tttggcagta gttaccattg ttttttgtgc
    36721 tgtactcgtc ttttttctaa atattccagg aaaaggtgtc ttaaaactcg ataatggaac
    36781 gattgtttat gatggcagtc ttgtccgtgg taaaatgaat ggccaaggta ccattacctt
    36841 ccaaaatgga gaccaatata caggtggctt caacaatgga gccttcaacg gaaaaggtac
    36901 ctttcaatct aaagaaggct ggacctacga aggtgatttt gtaaatggtc aggctgaagg
    36961 aaaagggaaa ctaacaacag aacaagaagt cgtttatgaa ggaactttta aacaaggcgt
    37021 ttttcaacaa aaataaagcc tccttatcaa aggaggtatt attagaatca caaggtaagc
    37081 gcttacctgt aaatcccttt ctttccaaat ccctcttcca agcaagtttg tgaaataaaa
    37141 aatatttgaa ataaatttca caaacttcaa agataaaact tgataagaaa agaaaatgag
    37201 aaaagtttca caagagttta aaaattcttt gtgaaatgtt tatgaaactg tttacaaagt
    37261 tgaaataatg cgttataata aacttgtgaa aaaattaaca aaggatatcg ttccttgaaa
    37321 gctatggagg aaaatatggc tgataaaaaa actgtgacac cagaggaaaa gaaactcgtt
    37381 gctgaaaaac acgtagatga gttggttcaa aaagctctag ttgcccttga agaaatgcgt
    37441 aaattggatc aagaacaagt tgactacatc gttgccaaag catcagtagc agctttggat
    37501 gcccacggag aattggcttt acatgccttt gaagaaacag gacgtggtgt atttgaagac
    37561 aaagcaacta agaacttgtt tgcttgtgaa cacgtagtaa acaatatgcg ccacactaag
    37621 acagttggcg ttatcgaaga agacgatgta acaggattga ctcttattgc tgaaccagtt
    37681 ggtgttgttt gtggtattac tccaacaaca aacccaacat caacagcaat cttcaaatca
    37741 ttgatttcat tgaagacacg taacccaatc gtctttgcct tccatccatc agcacaagaa
    37801 tcatctgctc atgcagctcg tatcgtccgc gatgcagcta tcgcagctgg tgctcctgaa
    37861 aactgtgtgc aatggattac tcaaccatct atggaagcaa caagtgccct tatgaaccac
    37921 gaaggtgttg cgacaatcct tgcaacaggt ggtaatgcca tggttaaggc ggcttattca
    37981 tgtggtaaac cagctcttgg ggtaggtgcc ggaaacgttc cagcttatgt tgaaaaatca
    38041 gcaaacattc gtcaagcagc acacgatatc gtcatgtcta aatcatttga taacggtatg
    38101 gtctgtgcat ctgaacaagc agttatcatt gataaagaaa tttacgatga atttgtagca
    38161 gagttcaaat cttaccacac ttactttgta aacaaaaaag aaaaagctct tcttgaagag
    38221 ttctgcttcg gcgtcaaagc aaacagcaaa aactgtgctg gtgcaaaatt gaacgctgac
    38281 atcgttggta aaccagcaac ttggattgca gaacaagcag gatttacagt tccagaagga
    38341 acaaacattc ttgctgcaga atgtaaagaa gttggcgaaa atgagccatt gactcgtgaa
    38401 aaattgtcac cagttattgc agttttgaaa tctgaaagcc gtgaagatgg tattactaag
    38461 gctcgtcaaa tggttgaatt taacggtctt ggacactcag cagctatcca cacagctgac
    38521 gaagaattga ctaaagaatt tggtaaagct gttaaagcta ttcgtgttat ctgtaactca
    38581 ccttctactt ttggtggtat cggggacgtt tacaatgcct tcttgccatc attgacactt
    38641 ggatgtggtt cttacggacg caactcagtt ggggataacg ttagtgccat taacctcttg
    38701 aatatcaaaa aagtcggaag acggagaaat aacatgcaat ggatgaaact tccttcaaaa
    38761 acatactttg aacgtgattc aattcaatac cttcaaaaat gtcgtgacgt tgaacgtgtc
    38821 atgatcgtta ctgaccatgc catggtagag cttggtttcc ttgatcgtat catcgaacaa
    38881 ctggaccttc gtcgcaataa ggttgtttac caaatctttg cggatgtaga accggatcca
    38941 gatatcacaa ctgtaaaccg tggtactgag attatgcgtg ccttcaaacc agataccatc
    39001 atcgcactcg gtggtgggtc tccaatggat gctgccaaag taatgtggct cttctacgag
    39061 caaccagaag tggacttccg tgaccttgtc caaaaattca tggatatccg taaacgtgcc
    39121 ttcaagttcc cattgcttgg taagaagact aaattcatcg cgattccaac tacatctggt
    39181 acaggatctg aagtaacacc atttgccgtt atctctgata aagcaaacaa ccgtaaatac
    39241 ccaatcgctg actactcatt gacaccaact gtggcaatcg tagatcctgc tttggtattg
    39301 acagttccag gatttgttgc tgctgatact ggtatggacg tattgactca cgcgacagaa
    39361 gcatacgtat cacaaatggc tagtgactac actgatggtt tagcacttca agccattaaa
    39421 ttggtctttg aaaatctcga aagctcagtt aagaatgcag acttccactc acgtgagaaa
    39481 atgcataacg cttcaacaat cgctggtatg gcctttgcca atgccttcct aggtatttct
    39541 cactcaatgg cccataagat tggtgcgcaa ttccacacaa tccacggtcg tacaaatgct
    39601 atcttgcttc catacgttat ccgttacaac ggtacacgtc cagctaagac agcaacatgg
    39661 cctaagtaca actactaccg tgcagatgaa aaataccaag atatcgcacg catgcttgga
    39721 cttccagctt ctactccaga agaaggggtt gaatcttacg caaaagctgt ctacgaactc
    39781 ggtgaacgta ttgggatcca aatgaatttt agagaccaag gaattgacga aaaagaatgg
    39841 aaagaacatt ctcgtgaatt agccttcctg gcttatgaag accaatgttc accagctaac
    39901 ccacgtcttc caatggtaga ccatatgcaa gaaatcatcg aagatgcata ctatggctac
    39961 aaagaaagac caggacgccg taaataattg tttatcagtc tagaagcaag acaaaaactc
    40021 aatttgaggg aaagatccag taatttttct atgataaaag gcatcctatc aaggtttttg
    40081 aacacctgat aggatgcctt tttatgatat tgaggccttt ttgccctttt tgaaaaacta
    40141 gaatagaaac aaaatatata atagattgaa actggaatag tacacttctg cttctaaaac
    40201 attgttagaa ttcgatttga ctgtc
//
LOCUS       DAVKFH010000014        39172 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKFH010000014 DAVKFH010000000
VERSION     DAVKFH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.014"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            147..281
                     /gene="rpmH"
                     /locus_tag="VW006_000771"
     CDS             147..281
                     /gene="rpmH"
                     /locus_tag="VW006_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9379654.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            565..1458
                     /locus_tag="VW006_000772"
     CDS             565..1458
                     /locus_tag="VW006_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9379655.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            1763..2536
                     /locus_tag="VW006_000773"
     CDS             1763..2536
                     /locus_tag="VW006_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9379656.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            2536..3096
                     /gene="rnmV"
                     /locus_tag="VW006_000774"
     CDS             2536..3096
                     /gene="rnmV"
                     /locus_tag="VW006_000774"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9379657.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            3168..4067
                     /locus_tag="VW006_000775"
     CDS             3168..4067
                     /locus_tag="VW006_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379658.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            4387..4683
                     /locus_tag="VW006_000776"
     CDS             4387..4683
                     /locus_tag="VW006_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9379659.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            4760..6790
                     /locus_tag="VW006_000777"
     CDS             4760..6790
                     /locus_tag="VW006_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9379660.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            6792..7433
                     /locus_tag="VW006_000778"
     CDS             6792..7433
                     /locus_tag="VW006_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379661.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            7426..7719
                     /locus_tag="VW006_000779"
     CDS             7426..7719
                     /locus_tag="VW006_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379662.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            7970..8842
                     /gene="rsmA"
                     /locus_tag="VW006_000780"
     CDS             7970..8842
                     /gene="rsmA"
                     /locus_tag="VW006_000780"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9379663.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            8844..9722
                     /gene="rsgA"
                     /locus_tag="VW006_000781"
     CDS             8844..9722
                     /gene="rsgA"
                     /locus_tag="VW006_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9379664.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            9733..10389
                     /locus_tag="VW006_000782"
     CDS             9733..10389
                     /locus_tag="VW006_000782"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9379665.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            10352..11014
                     /locus_tag="VW006_000783"
     CDS             10352..11014
                     /locus_tag="VW006_000783"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9379666.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            11016..12272
                     /locus_tag="VW006_000784"
     CDS             11016..12272
                     /locus_tag="VW006_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9379667.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            12262..13203
                     /locus_tag="VW006_000785"
     CDS             12262..13203
                     /locus_tag="VW006_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9379668.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            13470..14297
                     /gene="purR"
                     /locus_tag="VW006_000786"
     CDS             13470..14297
                     /gene="purR"
                     /locus_tag="VW006_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9379669.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            14364..15614
                     /locus_tag="VW006_000787"
     CDS             14364..15614
                     /locus_tag="VW006_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9379670.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            15740..16534
                     /gene="pflA"
                     /locus_tag="VW006_000788"
     CDS             15740..16534
                     /gene="pflA"
                     /locus_tag="VW006_000788"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9379671.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(16609..17535)
                     /gene="yidC"
                     /locus_tag="VW006_000789"
     CDS             complement(16609..17535)
                     /gene="yidC"
                     /locus_tag="VW006_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9379672.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(17613..17891)
                     /locus_tag="VW006_000790"
     CDS             complement(17613..17891)
                     /locus_tag="VW006_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9379673.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17925..18665
                     /locus_tag="VW006_000791"
     CDS             17925..18665
                     /locus_tag="VW006_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9379674.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18721..19404
                     /locus_tag="VW006_000792"
     CDS             18721..19404
                     /locus_tag="VW006_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9379675.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            19516..20508
                     /locus_tag="VW006_000793"
     CDS             19516..20508
                     /locus_tag="VW006_000793"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9379676.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            20573..21112
                     /gene="rsmD"
                     /locus_tag="VW006_000794"
     CDS             20573..21112
                     /gene="rsmD"
                     /locus_tag="VW006_000794"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9379677.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            21102..21590
                     /gene="coaD"
                     /locus_tag="VW006_000795"
     CDS             21102..21590
                     /gene="coaD"
                     /locus_tag="VW006_000795"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9379678.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGRDIACYVPESILEEIRNEK
                     KD"
     gene            21574..22611
                     /locus_tag="VW006_000796"
     CDS             21574..22611
                     /locus_tag="VW006_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9379679.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            22669..22899
                     /locus_tag="VW006_000797"
     CDS             22669..22899
                     /locus_tag="VW006_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9379680.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            22923..24206
                     /gene="murA"
                     /locus_tag="VW006_000798"
     CDS             22923..24206
                     /gene="murA"
                     /locus_tag="VW006_000798"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9379681.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            24199..24387
                     /locus_tag="VW006_000799"
     CDS             24199..24387
                     /locus_tag="VW006_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9379682.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            24426..25250
                     /gene="endA"
                     /locus_tag="VW006_000800"
     CDS             24426..25250
                     /gene="endA"
                     /locus_tag="VW006_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9379683.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            25479..26810
                     /locus_tag="VW006_000801"
     CDS             25479..26810
                     /locus_tag="VW006_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9379684.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRIAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(27008..27078)
                     /locus_tag="VW006_000802"
     tRNA            complement(27008..27078)
                     /locus_tag="VW006_000802"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27044..27046),aa:Cys,seq:gca)
     gene            27494..31105
                     /gene="rpoB"
                     /locus_tag="VW006_000803"
     CDS             27494..31105
                     /gene="rpoB"
                     /locus_tag="VW006_000803"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9379685.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            31138..34815
                     /gene="rpoC"
                     /locus_tag="VW006_000804"
     CDS             31138..34815
                     /gene="rpoC"
                     /locus_tag="VW006_000804"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9379686.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            34926..35339
                     /gene="ndk"
                     /locus_tag="VW006_000805"
     CDS             34926..35339
                     /gene="ndk"
                     /locus_tag="VW006_000805"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9379687.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(36268..36900)
                     /locus_tag="VW006_000806"
     CDS             complement(36268..36900)
                     /locus_tag="VW006_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379688.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(36902..37669)
                     /locus_tag="VW006_000807"
     CDS             complement(36902..37669)
                     /locus_tag="VW006_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9379689.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            complement(37720..>39172)
                     /locus_tag="VW006_000808"
     CDS             complement(37720..>39172)
                     /locus_tag="VW006_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9379690.1"
                     /translation="QINNDIVNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDN
                     LESSIVRKYEWDIDKVTGGGESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSK
                     NYVPNKGYLGKEEGEEGIISDIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFG
                     KSSASPDWIVKAIFDAVDDGNDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYA
                     NQKGVIIVAALGNDSLNVSNQSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDR
                     LGNLSDFSNKGDSDAIYAPAGSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYG
                     NSFAAPKVSGAIAMIIDKYKLKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDL
                     NMLQLEYKNEQSWDSFIDNVNLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPN
                     TSENTYNSLQVSLVGVLLLFISMVNILWAKKSK"
BASE COUNT        11562 a         7515 c         8863 g        11232 t
ORIGIN      
        1 agggcttgca ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga
       61 atcagataga atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat
      121 acaaaatcta tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc
      181 gtgcgcgtaa acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg
      241 cagctcgtcg tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa
      301 aaatcagtag gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca
      361 aatggattcg gaagcgatgt tatagtagat tgaaactaga atagtacacc tctgtttcta
      421 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tattttattt
      481 tactataaag ttgaagtagg tggagatggt acagcaacaa tcgtctttaa agatggttca
      541 gctattacaa ttccaggaaa tcaattggta gcacaagatc caaaagcaca agatagcact
      601 aaaccgactg ctgaaaaatc aactgttaaa gcacctgctc aaagagtaga tgtaaaagat
      661 ataactcatt taacagatga agaaaaagtt aaggttgcta ttttacaagc aaatggttca
      721 gcattagacg gagcgacaat caatgtagct ggagatggta cagcaacaat cacattccca
      781 gatggttcag tagtgacgat tctaggaaaa gatacagttc aacaatctgc gaaaggtgaa
      841 tctgtaactc aagaagctac actagagtat aagctagaaa ctataccagg tggagataag
      901 ggaggcaata ctggaagctc agatgctaat gcgaatgaag gcggtggtag ccaggcgggt
      961 ggatcagctc acacaggttc acaaaactca gctcaatcac aagcttctaa gcaattagct
     1021 actgaaaaag aatcagctaa aaatgccatt gaaaaagcag ccaaggataa gcaggatgaa
     1081 atcaaaggcg caccgctttc tgataaagaa aaagcagaac ttttagcaag agtggaagca
     1141 gaaaaacaag cagctctcaa agagattgaa aatgcgaaaa ctatggaaga tgtgaaggaa
     1201 gcagaaacga ttggagtgca agccattgcc atggttacag ttcctaagag accagtggct
     1261 cctaatgctg ctcctaagac aacaagtgca ccgcaagcaa ctgcaggaac aatgcaagat
     1321 gttacctacc agtcacctgc tggcaaacaa ttacctaaca caggttcagc atcaagtgca
     1381 gcacttgcta gtcttggtct agtggtggca acaagtggtt ttgctttgct aggaagaaag
     1441 actagacgta gaaaatagaa cagctagaaa attctattct ctacttaaag ttagattata
     1501 agggggattt tgagaagtca tcaatcctag tgatgggtga gaaaagtgag aacccaagat
     1561 aatcacatac tttagctgaa taggaatatt ctatcaatgt agccaatctc ttctgtctct
     1621 aactgtggaa taggagatgg gcaatatcgg atagaaaaga tagcagaata gctctctatt
     1681 gaagagagga ggggaaaccg aaaaattagg tgcccctcct cttttttggt ataatagaag
     1741 atagaaaacg aggttagaag agatgatttt tgatacacat acacacttga atgtagaaga
     1801 atttgcaggt cgtgaggcag aagaaattgc cttggctgct gagatgggtg tgatacagat
     1861 gaatattgtt ggttttgata aaccgacgat tgagcgtgcc ttggagttgg tagatgagta
     1921 tgagcagctc tatgcgacta ttggttggca tcctacagaa gctggtactt atacagagga
     1981 agttgaggct tacttgttgg ataagttaaa acattccaag gttgtggctt taggtgaaat
     2041 tggcttagac taccattgga tgacagcgcc caaagaggtg caggagcagg tttttcgccg
     2101 tcagattcag ctatctaagg acttggattt gccttttgtt gtccataccc gtgatgcgct
     2161 ggaagatacc tatgagatta tcaagagtga gggcgttggt cctcgtggtg gtatcatgca
     2221 ttcattttca gggacgcttg agtgggcaga gaagtttgtg gatcttggta tgaccatttc
     2281 cttctcagga gtggtgactt tcaagaaggc aactgacctc caagaagcag ctaaagagtt
     2341 acctttggac aagatgttgg tggaaacaga tgcgccttac ttagcacctg tacccaagcg
     2401 tggtcgtgaa aataaaacag cctatactcg ctatgtggtc gactttatcg ctgacttgcg
     2461 tggtatgacg acagaagagc tggcggtagc aacgactgca aatgcagaac gaatttttgg
     2521 attggacagc aagtaatgaa agagaaaatt tctcaagtta tcgtggttga aggtcgctat
     2581 gatacggtca atctcaagcg ttatttcgat gtggagacct atgagacacg aggttctgcc
     2641 atcaatgatc aggatataga gcgaattcag cgtctacacc aacgtcatgg agtcattgtc
     2701 tttacagacc cagattttaa tggggagcgg attcggcgca tgattatgac ggccattcca
     2761 acagttcagc atgcctttct caagcgagat gaagctgttc ccaagtccaa gaccaagggg
     2821 cgttctctgg gaattgagca tgccagctat gaagacctga aaacggctct agctcaggtg
     2881 acagaacaat ttgaacatga gagtcagttt gacattagtc gtagcgactt gattcgcctt
     2941 ggttttctag caggggcaga cagccgtaag cgtagagaat atctcggaga gactctccga
     3001 atcggctatt ctaatggcaa gcaactcctc aaacgcctag agttgtttgg ggttactttg
     3061 gcagaagtgg aagaagctat gaaatcttat gagtaggaaa gatgtagccg ttacaatttt
     3121 ttaagtttca cagtattttt cgaagcaggt agaagaggag gcgtctgatg ttaattggtc
     3181 aaaaaattaa agagattcgg atagaaaaag gaattagtcg tccagatttt tgtggagatg
     3241 agcaagaact gacagttcgt caactgtcgc gaattgaaag tggagcttcg caaccgagtt
     3301 tgcccaagtt agcctatatt gctcgccggc taggagttcc agtttatagc cttatgccgg
     3361 atttttcagc tcttccttct gcttatttag aattgaaata ccagatttta cgtgaaccaa
     3421 tctatggtaa agaagaggag tatgataaga aagaagcgtg tttggaagag atttataaaa
     3481 catactttga taatcttcct aaagaagaac aattagcatg tgaagtattg caggcgtgtt
     3541 tggatacttc tagaactaga aggcctgaat atgcagagtt aatacttgag gaacatatgt
     3601 ctcagatcat agaaaaagaa gtttattcaa caaatgatat gttgttgatt cgtttgtttt
     3661 tttatcaaat gctcattaga aaagatcttg ccaaatttat aaatcaaatc gaaaagctaa
     3721 tgctctttct tttggaacag aaaaaggtaa ctaaattaga aaacttcttt ataattagag
     3781 atactcttat ttcaggaatg tgttgtcttg aaaaggtagg agtaactgat tgttttaatg
     3841 attatctatc gtgtttacaa gaaattatgg ataaaactca agattatcaa aagaaacctc
     3901 ttgtatttat gtttttgtgg aagcaagcat taagagtaga aagagatttt agtttagctg
     3961 aatcatttta tcagtcttct aaaacatttg cgaagctaat tggagatgga tttctagtaa
     4021 agaaattgac agaggaatgg caagaggatg tcaaaaaata tttataaaca tagtgaatca
     4081 gtgacaaaaa tgtccttgtc ctcgtatcaa aacagttcta aagttcgtct ttagaactgt
     4141 ttttttagat ataagctaaa aataatatga aatagttaga ttttaaggac attgatgtcc
     4201 ttgatagtgt gataaagatg gagtataatg actataaaag ttcatttcag ggtgctattt
     4261 tgacaattta gagaaaaaca aataatttta gaatatcatt tggtaatata atagtatcaa
     4321 cgttgataat ggaaagagga gcaagtaact caacaatctt gtaatcaaaa atagaggagt
     4381 atcaatatga aacaaacagt taaaaagtta gctcttgtag cgagcattgc agcaacatta
     4441 ggtggaggtg tatcagttgc ctctgcagcc gttcaatacc cagaaggtgg tgtttggact
     4501 tatggttcag gtaacggtgg tgcttactca aactactatc acccttcaaa ataccatagt
     4561 tcaacagttg ttagcagaaa aactggttca tctgacaagg gatatgctgg tgctggaggg
     4621 acttctcgtg catggattcg tacttcttgg ggagagaaag ttgcattcta ctataatgtt
     4681 tagaatggta tagccctatt gtttagcttt acttggtcac cttctcacta agtgaccaag
     4741 taactttttg gaggaaatga tgaaacgttt atttattttg atttcaatgg tattggtatc
     4801 gctttatatg gtgataactt ccgttgacca tcgagaagag attttatttg gtaactatcc
     4861 ttctgttgat gtgacaggaa tgatgataaa tcaaccagta gctagtcgcg aagaggtgac
     4921 agaggctttg agtcacttgg cggtagagca taatagtctc atcgcccgtc gaatcgttga
     4981 gccaaatgaa gctggagaaa cactctttac ctatgccact tatggtgagg gagagcttcc
     5041 agaaggtctg accatttcct ccaaggagag tgcagaaacg agtgatttat tagggtctta
     5101 cttgattgtg tcaggaagtt tggatggagt gagcttacag accaccttga aagagcttgg
     5161 ttatcaaggc tttgtttcga atggagaaga tccattttcg atagtcttac tattggcggc
     5221 cacccctatg gtgctactga gtttagctat ttttctgctg acctttatga gtctgaccct
     5281 gatttatcgg atcaaatccc ttcgtcaggc agggattcgc ttaatagctg gtgagagctt
     5341 gtttggagtt gctctcagac cagtgttaga agatgtgaga cagcttatct gctcagtgct
     5401 ggtatccagt cttttgggat tggggattct ctggtatcaa ggtgccttgt ttatggcaac
     5461 ggtacaactg gtcatcattg ctcttctact ttatggattg accttggcag ggatttctac
     5521 cttactaagt gtcgtctatc tacttggttt acaggaaaat agtctggtgg atctattgaa
     5581 agggaaactc cctctcaaac gtatgatgac attgatgatg gtggggcaac tcttagctgt
     5641 attggtggtc ggatcgagtg cgacagctct cctaccccac taccgtgaaa tgcaggaaat
     5701 ggagagagct agcaataaat ggagccagtc ctcagaccgt taccgtctat cctttggttg
     5761 gtctagtgca tttgccgatg aagaaggaac gcgtaaggat aatcgtgagt ggcagacatt
     5821 tactgaagaa cggttagcca atacagactc tttttatatt atgagcaatg ttgacaattt
     5881 ctcagatgga gcagaagtgg acctagatgg caatcgtctc agtgactaca caccgttagg
     5941 gaatgttatc tatgtctcgc cgcgctatct gatagaagaa aagattaccg tttcttcaga
     6001 gtttatggac aagatgcaaa acttgtctga gggagagttt gggctgatct tgcctgagag
     6061 cttgcgagag cagtctgcct actaccaagg attgtttaca gattacctgc aaaacttttc
     6121 atctgaaagt gtagaagtga cgagtcagaa acactacctc ccacaggtaa ggctagcttt
     6181 tacagaaaca ggacaggaac gtttcctcta taatgatggg tacaagacaa cacgccagta
     6241 cctaaaagat ccgattattg tagttctaac gccgcaagcg actggaacaa gacctgttgc
     6301 agggatgttg tggggaacta cggctaatag tgccttgaaa ctagatcgat atggagacag
     6361 catcacagct ctaaaagagc aaggtctgta tcacaaggtt tcttacttgg taaaaagcca
     6421 gctatttttt gccaaggtac taaatgacaa acgggtggag ttttactctc tccttattgg
     6481 gacgattttg accctgtcta cggctatctt gttatttgat tccatgaatc ttctctattt
     6541 tgagcagttc agacgggaac ttatgattaa acgtcttgct ggtatgacaa tctatgagct
     6601 tcatggcaag tatttactgg cgcaaggagg agttctcttg cttggcctag tcctatctag
     6661 tattttgaca agagatggtt tgattagcgc tctagttgta gctttgttta cgcttaacgc
     6721 cctcttgatt ttagtaaggc aggataaaaa agaagaagct ggtagcatgg cagtattgaa
     6781 aggaaaataa gatgattgat attcaaggat tggaaaagaa atttaatgac cgcgcgattt
     6841 tctctggttt gaatctcaag ctggagaagg gcaaggttta tgccttaatc ggaaagagtg
     6901 gaagcggaaa gacgacgctg ctgaatatct tgggaaagct agaaaagata gatggtggaa
     6961 gggttctcta tcaggggaaa gatttaaaaa ccattcctac tcgtgagtat tttcgagacc
     7021 agatgggcta tctctttcaa aatttcggcc tcttagaaaa ccaatcaatc aaagaaaatt
     7081 tggatttggg ttttgttggt cagaaaatct caaaagtaga acgtttggaa aggcaagtgg
     7141 gggctttaga aaaagttaat ctagggtatt tggatttaga acaaaaaatt tatactttat
     7201 ctgggggaga ggcccaacga gttgcccttg ctaagactat tttgaaaaat ccacccttga
     7261 ttttggcaga tgaaccaaca gcagctcttg atcctgaaaa ttcagaggag gttatgaatc
     7321 tcttggtgga tttgaaagat gaaaatcgaa ttatcatcat tgcgacccat aatcccctag
     7381 tctggaataa ggctgatgaa atcattgata tgaggaaact tgctcatgtg tgaaaaaatc
     7441 cgtattcgca gggtatctga ttatcctagt gccagaggtg gtttagaaga tatcctcatc
     7501 atggaaaata tgaccaatca tctccttttg gttcaaatcc gagtgcatgg ctatttgctt
     7561 gattttgcta gtattgaagg gcaaaggcaa aagcattatc gtttgaaaaa tttacctcag
     7621 acggttgaac tgacagtgga tgatgtggag gaggatgtgg atttgaccct acccgaaaat
     7681 cgaagttatc aagaagctga tttttttgat actcaatgaa aatcaaagag caaactagga
     7741 agctagccgc aggctgtact tgagtacggc aaggtgaagc tgacgtggtt tgaagagatt
     7801 ttcgaagagt atgaacgtat gtttcgagag aagcactaag tccctcttta aagatttcca
     7861 agactatctt tcttcatgag gaaagatagt tttttggtat gattttcatt cccaaaatac
     7921 aaggggaatg tgttacaata gtagtaacag ataatagaaa agagaataga tgagaattgc
     7981 agattatagc gtgaccaagg cagtgttgga gcgtcacggt tttaccttta aaaagtcctt
     8041 tgggcaaaat ttcttgacgg ataccaatat ccttcaaaaa attgtggata cggctgaaat
     8101 tgatgatcag gtcaatgtca tcgaaatcgg gccaggtatt ggtgccttga cagaattttt
     8161 ggctgagcgt gcagcccaag tcatggcttt tgagattgac caccgtttga tgccaatttt
     8221 ggcagatact ctgcgtgatt ttgataatgt gaccgtagtt aacgaagata ttctcaaggt
     8281 tgatttggcg caacatatcc aaaattttaa aaatcctaac ctgccaatca aggtagtggc
     8341 taatttgcct tactacatca cgacgcctat tctcatgcac ttgattgaga gtggcattcc
     8401 tttttgtgag tttgtggtca tgatgcagaa agaagtagcg gaccgcattt cagcccagcc
     8461 taacaccaag gcttacggta gcttgtctat cgccgtgcag tattacatga cagccaaggt
     8521 tgcctttatc gtgcctcgta cggtctttgt gccagcgcca aatgtggatt cagccatctt
     8581 gaaaatggtg cgtcgtccag agccagccgt agcagtagaa gatgagaact ttttctttaa
     8641 ggtttccaag gctagtttta cccatcgccg caagaccttg tggaataact tgacaggtta
     8701 ctttggtaag actgaagagg tcaaggacaa gctgaccaag gctttggacc aggcaggctt
     8761 gtcaccaagt gtgcgtgggg aagctctcag cttggcagaa tttgccggtc tagcagacgc
     8821 acttaaaggg caaggactct aagatgcagg gacaaatcat taaagccttg gcaggtttct
     8881 actatgtgga gagtgatggc caggtttatc aaacacgcgc gcgtgggaat ttccgtaaaa
     8941 aaggccatac cccttatgtt ggggactggg tagatttctc tgccgaggaa aattcagaag
     9001 gctatatcct caaaattcac gaacggaaaa acagtctggt tcgtccgcct attgtcaata
     9061 tcgatcaagc tgtagtaatc atgtccgtca aggaacctga ttttaacagc aatttgctgg
     9121 atcgtttctt ggttcttttg gagcacaagg gcatccatcc cattgtctat atttccaaaa
     9181 tggatttgtt ggaagatagg ggagaactgg atttttacca gcagacctat ggtgacatcg
     9241 gctatgactt tgtgaccagt aaagaggaac tcctgtcttt gttaacaggc aaggttacgg
     9301 tctttatggg gcagacaggt gttgggaagt caactcttct caataaaatc gcaccagacc
     9361 tcaatcttga aacgggagaa atttcagaca gtctaggtcg cggtcgccat accactcgag
     9421 ctgttagttt ttacaatctc aacgggggta aaatcgcaga tacaccaggc ttttcatctc
     9481 tggactatga agtatcaagg gctgaagacc tcaatcaggc ctttccagag attgctactg
     9541 ttagccgaga ttgtaagttc cgtacttgta cccataccca tgagccgtct tgtgccgtca
     9601 aaccagctgt tgaagagggt gttattgcaa ccttccgttt tgacaattac ctgcaattcc
     9661 ttagtgaaat tgaaaatcgt agagaaacct ataaaaaagt cagcaaaaaa attccaaaat
     9721 aaggagaaac ctatgtctca atacaagatt gctccgtcaa ttctggcagc agattatgcc
     9781 aactttgaac gtgaaatcaa acgtctagaa gcaactgggg cagaatatgc ccatatcgat
     9841 atcatggaca gtcattttgt accgcaaatc agttttggtg caggtgtggt cgagagcctt
     9901 cgtcctcata gtaagatggt tttcgattgc cacttgatgg tgtcaaaccc tgagcatcat
     9961 ctggaagatt ttgcgcgtgc aggtgcagac atcatcagta tccatgtaga agcaacgcct
    10021 catattcatg gcgccctcca aaaaattcgt tcactcggag ttaagccttc agtcgttatc
    10081 aatcctggca catcagttga agccatcaag cacgtccttc atctagttga ccaagtttta
    10141 gtcatgacgg ttaatccagg ttttggtggg caagcctttc tgccagaaac catggataag
    10201 gttcgtgagt tggttgctct tcgtgaggaa aaaggtttga actttgaaat cgaagtggat
    10261 ggtgggattg atgaccaaac tattgcccaa gccaaagaag ctggtgcgac cgtttttgta
    10321 gcaggttcct atgtctttaa gggagatgtc aatgagcgag tacaaactct cagaaaacaa
    10381 ctggactagg gttgccgttt ttgcaggcgg agaccgcggt cattatcgga cagattttga
    10441 tgcttttgtt ggggtggatc gaggctcgct ctgggtcttg gaagaagact tgcttcttgc
    10501 tctagcagtt ggggattttg attctgtgac cgaagaagag cgacaggtga ttcaaaaacg
    10561 tgcccagcat tttgtccaag cccgaccaga aaaagatgat accgatttgg aattggctct
    10621 cttaaccatc tttgagaaaa atcctcaggc tcaggttact attttcggtg ccttgggtgg
    10681 ccgtattgac catatgttgg ccaatgtctt tctgcctagc aatcctaagt tggcacccta
    10741 tatgcatcaa atagaaattg aggatgggca aaacttgatt acttattgtc cagaagggac
    10801 cagtcagcta gaacctcgtt cagactacga ctatctagcc tttatgccag ttcgggatag
    10861 ccagctgact attcttggag ccaagtatga gttgacagag gaaaattttt tctttaaaaa
    10921 agtgtacgct tctaacgaat atatagatag ggaagtgtcg gtaacttgcc cagatggtta
    10981 tgtggtcgta ctgcatagca aggacaggag gtaggatgga aagtttactt attctattat
    11041 taattgccaa cctagctggt ctctttctga tttggcaaag gcaggatagg caggagaaac
    11101 acttaagtaa gagcttggag gatcaggcag atcatttgtc agaccagttg gattaccgct
    11161 ttgaccaagc cagacaagcc agccagttag accaaaaaga tttggaagtg gttgtcagcg
    11221 accgtttgca agaagtgcgg attgaattgc accaaggtct gacccaagtc cgtcaagaaa
    11281 tgacagataa tctcctccaa actagagaca agacagacca acgtctccaa gccttgcagg
    11341 aatcaaatga gcaacgtttg gaacaaatgc gccagacggt cgaggaaaaa ctagaaaaga
    11401 ccttgcagac acgcttacag gcttcctttg agacagtttc taaacaactg gagtctgtca
    11461 atcgtggcct tggagaaatg cagacagttg cccgtgatgt cggagctctt aacaaggttc
    11521 tctctggaac caagacgcga gggattctgg gagaattgca actggggcaa attattgaag
    11581 acatcatgac acctgcccag tacgaacgag aatacgcaac ggttgaaaac tctagtgaac
    11641 gagtggagta tgccatcaag ttacccggac aaggcgacca agaatacgtc tatctgccaa
    11701 ttgactctaa gtttccactg gcagattatt accgcttgga agaagcctat gagacaggtg
    11761 acaaggatga gattgaacgc tgtcgtaagt cactcctagc aagcgtcaag cgctttgcca
    11821 aggatatcaa gagcaagtac atagcaccgc ctcggacgac caattttgga gttttgtttg
    11881 ttccgacaga aggtctttat tcagagattg tgcgcaatcc ggtcttcttt gatgatttga
    11941 gacgggaaga acagattatc gttgcaggac caagtaccct atcagccctt cttaactccc
    12001 tatcagttgg tttcaagacc cttaatatcc aaaagagtgc cgaccatatc agcaagactc
    12061 ttgccagtgt caagaccgag tttggcaagt ttggtggtat tctggtcaag gcacaaaaac
    12121 atctccaaca tgcctctggc aatattgatg aattattaaa ccgtcgtacc atagctatcg
    12181 agcggacgct ccgtcacatt gagttgtcag aaggcgagcc tgcgcttgat ctactccatt
    12241 ttcaagaaaa tgaggaagaa tatgaagatt agtcacatga aaaaagatga gctatttgaa
    12301 ggcttttacc taatcaaatc agctgacctg aggcaaactc gagctgggaa aaactaccta
    12361 gcctttacct tccaagatga tagtggcgag attgatggga agctctggga tgcccaacct
    12421 cataacattg aggcctttac cgcaggtaag gttgtccaca tgaaaggacg ccgagaagtt
    12481 tataacaata cccctcaagt caatcaaatt actctccgcc tgcctcaagc tggtgaaccc
    12541 aatgacccag ccgatttcaa ggtcaagtca ccagttgatg tcaaggaaat tcgtgactac
    12601 atgtcgcaaa tgattttcaa aattgaaaat cctgtctggc aacggattgt ccgaaatctc
    12661 tacaccaagt atgataagga attctactcc tatccagctg ccaagaccaa ccaccatgcc
    12721 tttgaaacgg gcttggccta tcatacggcg accatggtgc gtttggcaga cgctattagc
    12781 gaagtttatc ctcagctcaa taagagcctg ctctatgcgg ggattatgtt gcatgactta
    12841 gctaaggtca tcgagttgac ggggccagac cagacagagt acacagtgcg aggtaatctt
    12901 cttggacata tcgctctcat tgatagcgaa attaccaaga cagttatgga actcggcatc
    12961 gatgatacca aggaagaagt cgttttgctt cgtcatgtca tcctcagtca ccacggcttg
    13021 cttgagtatg gaagcccagt ccgtccacgc attatggaag cagagattat ccatatgatt
    13081 gacaatctgg atgcaagcat gatgatgatg tcaacagctc ttgctttggt ggataaagga
    13141 gagatgacca ataaaatctt cgctatggat aatcgttcct tctataaacc agatttagat
    13201 taataattta agaaaaatga gcatttttta ggataagaat gttcgttttt ttatgtgaat
    13261 atggtataat aagtaaaaga caaaaatgaa tactcttcga aaatctcttc aaactagggt
    13321 agtatcgcct tgtcgtatgt atatatgcag gtatattaca gggtttgtca gttctattga
    13381 caatctcaaa acagtgtttt gaaccaccag cgaccagctt tctagtttgc tttttgattt
    13441 tttgaataaa aatggaatag gaaatagaaa tgaaattaag aagaagtgat cggatggttg
    13501 tcatttccaa ctatttgatt aataatcctt ataaactaac tagtctcaat acttttgctg
    13561 aaaagtatga gtctgctaaa tcatccatct cagaagatat cgtcattatc aaacgcgcct
    13621 ttgaggaaat tgaaatcggt catatccaga cagtgactgg ggctggcgga ggtgtcatct
    13681 tcacaccgtc tatttcgagt caggatgcta aggaaatggt tgaagacttg cgtaccaagt
    13741 tgtcagaaag tgaccgtatc ttgccaggtg gttatatcta tctgtctgat ttgcttagca
    13801 caccagccat cttgaaaaat attggtcgta ttattgccaa aagctttatg gaccaaaaaa
    13861 ttgacgcggt tatgaccgta gcaactaagg gtgtgccact tgcaaatgca gttgccaatg
    13921 tcctcaatgt ctcttttgtc attgtgcgcc gtgacctgaa aattaccgaa ggttcaactg
    13981 ttagcgtcaa ctatgtttca ggttcaagtg gtgaccgtat cgagaaaatg ttcctttcaa
    14041 aacgtagtct taaggcaggt agccgtgtct tgattgtgga tgacttcttg aaaggtggcg
    14101 gaacggtcaa cggtatgatt agtctcttgc gcgagttcga ctcagaactg gcaggtgtag
    14161 cggtctttgc ggacaatgcc caagaagaac gtgaaaagca gtttgactac aagtcactct
    14221 tgaaggtaac taatattgat gtcaagaacc aagccatcga tgttgaggtt ggcaatatct
    14281 ttgacgaaga taaataagag atagaactaa aggttggaac gattgtccca gcctttcttt
    14341 gcaaacagaa tagaaggaag cttatgaaaa caccatttat caatagagaa gagttagaag
    14401 cgattgttgc cgagttcccg actccctttc acttgtatga tgagaagggg attcgtgaga
    14461 aggcaagagc cgtcaaccaa gctttttcgt ggaacaaggg ctttaaggaa tattttgcag
    14521 ttaaggctac tccaactcca gctattttga aaattctcca agaagaaggt tgtggtgtgg
    14581 actgctctag ttatgtagag cttttgatga gccataaact ggactttctg ggttctgaga
    14641 ttatgttctc ttccaacaac acgccagaca aggaatacgc ctatgcacgt gaattgggtg
    14701 cgaccattaa cttggatgcc tttgaagata ttgaacatct ggagagagta gcaggcattc
    14761 cagaaatcat ctcttgtcgt tataatcctg gaggcgtttt tgaactgggg acagacatta
    14821 tggacaatcc tggggaggct aagtttggca tgaccaagga ccagctcttt gaagcctttg
    14881 ctatcttgaa ggaaaaagga gccaagactt ttgggattca ctccttccta gcgtccaata
    14941 ccgtgaccca tctctattat ccagagttgg ctcgtcagct ctttgaactg gctgttgaaa
    15001 tcaaggaaaa gttgggcatt tggctagact ttatcaatct ttctggcggt attggtgtta
    15061 attatcgtcc agaccaggag ccgaacgata tcgccttgat tggtgaggga gttcgtaagg
    15121 tgtatgaaga agttcttacg tcagcaggtc ttggtcaggt caagattttc accgaattgg
    15181 gtcgttttat gctggcacct cacggtgctc tagtcacaag agtgactcat aagaaggaaa
    15241 cctaccgtac ctatctaggt gtggatgcct cagcagtcaa cctcatgcgt ccagctatgt
    15301 acggagctta ccatcatatt agcaacgtga cccatccaga tggaccagct gaagcggtag
    15361 atgtggttgg ttcactctgt gaaaacaatg ataaatttgc agtcaatcgc gaactgcctc
    15421 atacagaaat cggtgatttg ctggtaattc atgatacagg tgcccacgga ttttcaatgg
    15481 gctaccagta taatgccaaa ttacgttctg cggaaatcct ctataccgaa gaaggtaaag
    15541 cccgtcaaat ccgccgtgca gagcgccctg aggactattt tgcaacctta tatggcttcg
    15601 attttgaaga ataatctgat aatagattga aaatgaaatt gaaaaacaga ttgctttcta
    15661 aaaaataggc aaaaatcttg tttttccttc aagtcgtgat ataataaaac tataaaacgt
    15721 tttcaaggaa ggtaacgata tgtctgaaga aacaattgat tatggacaag tgacaggaat
    15781 ggtgcattcg acagaaagct ttgggtcagt agatgggcct ggtattcgct ttattgtctt
    15841 tttgcagggc tgtcacatgc gttgccagta ttgccacaac ccagacactt gggctatgga
    15901 gtccaataag tcacgtgaac ggacggtaga tgatgtcttg acagaggcct tgcgctaccg
    15961 tggtttctgg ggaaataagg gtgggattac agtcagtgga ggagaagctc tcttgcagat
    16021 tgatttcctg attgctctct tcaccaaggc taaggaacaa ggaatccact gtaccttgga
    16081 tacctgtgct cttcctttcc gtaataaacc acgttacctt gagaagtttg acaaactcat
    16141 ggctgtcact gacttggttc ttttggatat caaggaaatc aacgaagaac agcacaagat
    16201 tgtcactaga caaaccaata aaaatatctt ggcttgtgcc cagtatctat cagatattgg
    16261 aaaacctgtc tggattcgcc acgtgctagt tccaggattg acagacagag atgatgactt
    16321 gattgaactt ggtaagttcg tcaagaccct caaaaatgtt gataagtttg aaattctacc
    16381 ttatcacacc atgggtgagt tcaagtggcg tgaacttgga attccatatt ccctcgaagg
    16441 agtcaaacca ccaacagcag atcgcgtcaa gaacgctaaa aaactcatgg ataccgaaag
    16501 ttatcaagat tatatgaaac gtgtacatgg atagaaaaga agcctgatgg aaacatcggg
    16561 cttttgattt gcaaaaagac ttagcaaatc agctaagcct ttttcttctt atctcgaacg
    16621 ttgttttcca gcgttgcgat ttttgtgttt tttcttgctt gtgatagcag ttggttgttc
    16681 aggggtaacg tcttttcgtc cacttggttt agagaaagca cttgcttttg gtgggttctt
    16741 ggctagttct tcacggactt ttttgcgaag ttttggacga acgatatagt tgacgataaa
    16801 ctgttggaga atcatcatga aaccaccgac aacccagtaa agtgtgacac tagctggtga
    16861 gaagagggag aagacgacga tcatgagtgg gctcatgtaa atcattttct tgatttgttc
    16921 tctttgcatt tcatcttcta ctccgtgaag tgaaaggagc gattgaagat agtaaaggac
    16981 accagcacag gcaaccaaaa tcatacttgg agaacctaga ggaatgccta ggtagcttgc
    17041 ttgagcaacc ccttcagtat gttgggcagc aaagtagata gcagagaaga aaggcatttg
    17101 aaggaggata gggaaacatc ctacaccgcc aaacatgctg ataccgtgct ctttttgagc
    17161 agcaaagaga gcttgttggg cttcgagttt ttcttcttga gtagtcgctt ctttgagacg
    17221 cgtttggtgt ggctcaagga cgtgcttgag ggcgttcatc ttttcagagt gaagcgttgc
    17281 cttccatgat tggtagatac caagtggcaa gataatcaag cgcacgataa tggttacgat
    17341 gatgatagcg acaccaaagc ctagaccttt atcagtagcg aagtacttga tagcttcagc
    17401 cataggggct ccgatggtat tccaaataaa tcctgttggc tgacctgtgg ttttatcgac
    17461 attgacacaa ccagtcaaga caagcaacat agccgctccc atagctgaga gtgcaaaacg
    17521 tttaatagat ttcactgttt ttattccttc tttaaaaatt atacctttct attctactgt
    17581 ttttttacaa aatatacaat agttctggag acctaatttg cgattttaaa gtcagagtag
    17641 ggaggaaagt tgcttagttt gacatctaag tagcttactt ttgaaaaagg tgtcggtcct
    17701 tttcggattt cttggataaa ttttgccatg atagcagatg agtctgcttg ggctaagatt
    17761 tccactgtgc catcgtcgtt attccatact cgacctgtga tgccaccaat ttcaagtgcc
    17821 aagctgtaaa caccccaacg aaagccgact ccctgtaccc taccttgggc aatcattcta
    17881 accttttgca taccaaaccc ctttgattgt gttataatat ttctatgact attataacct
    17941 caaaagccaa ttctgtggta aaaaatgcca agaaattacg ccaaaaaaaa taccgcaagt
    18001 ctgcctactt gattgaaggc tggcacttgt ttgaagaagc tgtccaagct ggagtgacga
    18061 ttgagaaaat ctttgcccta gaaaattatc gagatcagtt agctactttt tcgcaaactg
    18121 tctgggtgtc agaggatatt ttgctagatt tggcggattc tcagactcca cagggaattg
    18181 ttgccgtggt tcaaaaagaa gaagtaggac aagctgattt gagtcagggc aagttcttgt
    18241 ttttggaaga tgtgcaagat cctggtaatg taggaactat cattcgaact gcggatgcag
    18301 caggttttac aggagtgatt gtttcagata agtcggcaga catctacagt ctcaaaaccc
    18361 tacgttccat gcaaggtagt catttccatc tgcccattta ccggatgact agtcaagcgc
    18421 ttcttgacga aactaaaaag gtagctatcc cagtgctagc aacaacccta tctaaagatt
    18481 ctgttgatta cagagaactg ccttctatag aaaattttgt actagttatg ggaaatgagg
    18541 gtcaaggaat tagtcccctt atggctgaaa gtgcagacca gttggtccat attagcatga
    18601 aggggcaggc cgagagtttg aatgttgcgg ttgcagccgg tattttaatc ttccatttaa
    18661 gctaatttta actttttttg ttataatcaa ggaaagatgt tcacagaaaa ggagaaatgg
    18721 atgaatcaca ctattataca tgaccgtgca ggtctcaatc aattttacgc taaggtttat
    18781 gcctttgttg gtctgggaat cggactatct gctttggtat caggccttat gttgacggtc
    18841 tttcagtctc agttggttta ctttttgatg caggggcgtc tctggttgac cattgctact
    18901 tttgcggagt tggctttggt tttcgttgcc agtagcatgg cttcaagaaa tagtccagct
    18961 gctcttccag tatttttact ttactccgtt ttaaacggct ttacccttag ttttgtggtg
    19021 gctttctata ctccgggtac agttttatct gcctttgtat cgagcgccct tctcttcttt
    19081 gtcatggcgg cagttggtat ttttaccaag aaagacttga gtgggattgg tcgagcaatg
    19141 atggcagctc tcattggttt actgattgct atggtagtca atattttctt ggctagtggc
    19201 ttctttgatt atatgattag cgtagccatg gttttggttt tttcagggtt gattgcttgg
    19261 gataaccaaa agattcgcct cgcctatgaa caatcacaag gtcgtgtagc gacaggctgg
    19321 gtggtgtcaa tggctctcag catctatctt gattttatca atcttttcct aagtattcta
    19381 cgtatctttg gtcgcaatga ctaaaaggtc tatgaaatca gtgttttgag tgacactgat
    19441 tttttttaaa ttttctcttt tcttttcttg gaaataggtg tataatacgt ttattaaatt
    19501 tttgaggagt tgtctatgaa gaaaagtttt atccatcaac aagaagaaat ttcctttgtc
    19561 aaaaacactt ttacccagta tttgaaagat aagctagaag ttgtcgaagt tcaaggtcct
    19621 atcttgagta aggtcggtga cggaatgcag gacaacctgt ctggtgtgga aaatccagta
    19681 tcggtcaagg ttctccaaat ccctgatgct acttatgaag tggtgcactc acttgctaaa
    19741 tggaaacgcc acaccttggc tcgttttggc tttggtgaag gagagggtct ctttgtccac
    19801 atgaaagccc ttcgtccaga tgaggattcc ttggatgcaa cccactctgt ttatgttgac
    19861 cagtgggact gggagaaggt tatcccaaat ggtaagcgta acatcgttta tctaaaagaa
    19921 acagttgaga agatttataa ggctattcgc ctgactgagc tagctgttga agcccgctat
    19981 gacatcgagt ctatcttgcc aaaacaaatc acctttatcc atacagaaga attggtagaa
    20041 cgctacccag acttgacacc gaaagaacgt gaaaatgcga tttgtaaaga atttggagcc
    20101 gtctttttga ttggtatcgg tggcgagttg ccagatggta aaccgcacga tggacgtgca
    20161 ccagactatg atgactggac aagcgagtct gagaatggct acaagggtct aaatggtgat
    20221 attcttgtct ggaatgagtc tttaggtgga gcctttgagt tgtcttctat gggaattcgt
    20281 gtagatgaag aaactcttag acgtcaggtg gaaattacag gtgatgaaga ccgcttggaa
    20341 ttggaatggc acaagtcttt gttgaatggt ctattcccat tgacaatcgg tggaggaatt
    20401 ggacaatctc gtatggccat gttcctactt cgcaagagac acatcggaga agtgcaaaca
    20461 agtgtttggc ctcaagaagt ccgcgatact tacgaaaata ttttgtagag aatcgaaccg
    20521 caaggttcgg ttttctttct ctttttgtct ataatttggt ataataaaca gtatgaaaat
    20581 cgtatcagga atctatgggg gacgtcccct caagacacta gaaggcaaga cgacaagacc
    20641 tacttcggat aaggttaggg gagccatttt taacatgatt ggtccctact ttgaagtggg
    20701 acgagtcttg gacctttatg caggtagtgg tggtttatct atcgaagcag tatcgcgtgg
    20761 catgtccagt gctgttttgg tggagcgaga ccgtaaggct cagaccatcg tggctgaaaa
    20821 tatccagatg accaaggaag ttggaaaatt tcaactccta aagatggatg cagaaagggc
    20881 attggaacag gtatctgggg aatttgacct cgttttctta gaccctccct atgccaagga
    20941 acaaatcgta gcagatattg aaaaaatggc tgagagagag cttttttctg aagatgttat
    21001 ggttgtgtgc gagacggata aagccgttga acttccagaa gaaattgcct gtctgggtat
    21061 ctggaaggaa aagatttatg gaattagtaa ggtgacagtc tatgtcagat aagattggct
    21121 tattcacagg ctcatttgat ccgatgacaa atgggcatct ggatatcatt gaacgggcga
    21181 gcagactttt tgataagctt tatgtgggta ttttttttaa tccccacaaa caaggatttc
    21241 tccctcttga aaatcgtaaa cgggggctag aaaaggcttt gggacatttg gaaaatgttg
    21301 aagtcgtggc ttctcatgat gaattggtgg tcgatgttgc aaaaagattg ggtgctactt
    21361 gtctagtgcg tggtttgagg aatgcgtcgg atttgcaata tgaagccagt tttgattact
    21421 acaatcatca gctgtcttct gatatagaga ctatttattt acatagtcga cctgaacatc
    21481 tctatatcag ttcatcaggc gttagagagc ttttgaagtt tggtcgggat attgcctgct
    21541 atgttcccga gagtattttg gaggaaataa gaaatgaaaa aaaagattag atggccctta
    21601 tacgtcattg cggccttgat tgtgactttc ttggcatttg tagtgccctt gccttattat
    21661 atagaggttc caggtggttc ggaagatatt cgccaagtcc ttaaaataaa tgacacagaa
    21721 gataaggaag ctggtgccta tcaattcgtt acggttggtg ttcaacatgc cactttagct
    21781 catatgattt atgcttggtt gacacctttt acagatattc gtagtgctca ggagactaca
    21841 ggtggttctt ccgatgttga atttatgcga atcaatcaat tctacatgca aacatcgcaa
    21901 aatatggcca agtatcaagg actaaaaaca gctggtaagg atatcgaact caagtacttt
    21961 ggagtttatg ttttgaatgt gacggataat tcaaccttta aagggattct caatatctct
    22021 gatacagtca cagcagtcaa tgatcagacc tttgatagtt ccaaagactt gattgattac
    22081 gtcagttctc aaaaattagg ggattccgtc aaggtcacct atgaagagga tgggcaaacc
    22141 aagtctgcag aaggaaagat tatcaccttg gaaaatggca aaaatggaat tggaatcggc
    22201 ttgattgacc gtacagaggt aatcagcaat gtcccaatta gcttttcaac agctggtatt
    22261 ggcggtccaa gtgctggtct catgtttagt ctagctatct atactcaaat agctcaccca
    22321 gatcttcgta atggtcgtat tgttgccggt acaggtacca ttgaccgcga tggtaatgtg
    22381 ggagacattg gaggtattga taagaaggtt gtagcttcgg ctagggcagg tgctgctatt
    22441 ttctttgctc ctgataaccc tgttagcgaa gaagaacaaa aggcgcatcc ggacgcgaaa
    22501 aacaactacc aaacagccct agaagcggct aaaacaatca agacggatat gaaaatcgtg
    22561 cccgttaaaa ccctacaaga tgcgattgat tacttgaaaa acaatcccta atttttgagt
    22621 gaagtttcca aactagcgca caaacagaga agatggtata atagtcaaat ggttcaatta
    22681 ttattcactc taagtagtca tatgctcttt atttatgtga gtttttacct tttaaaggat
    22741 cttgttagat gggagaaggt tttaaaagtg acagatgaga atacaagaaa agttcgttta
    22801 ttagtagcct tttttagcat tgtcataggc tacatcctga gttctttctt tattagcctg
    22861 tatcatttgt ggcaagaagc gcttagagga ttattatgaa atcaagagta aaggaaacga
    22921 gtatggataa aattgtggtt caaggtggcg ataatcgtct ggtaggaagc gtgacgatcg
    22981 agggagcaaa aaatgcagtc ttacccttgt tggcagcgac tattctagca agtgaaggaa
    23041 agaccgtctt gcagaatgtt ccgattttgt cggatgtctt tattatgaat caggtagttg
    23101 gtggtttgaa tgccaaggtt gactttgatg aggaagctca tcttgtcaag gtggatgcta
    23161 ctggcgacat cactgaggaa gccccttaca agtatgtcag caagatgcgc gcctccatcg
    23221 ttgtattagg gccaatcctt gcccgtgtgg gtcatgccaa ggtatccatg ccaggtggtt
    23281 gtacgattgg tagccgtcct attgatcttc atttgaaagg tctggaagct atgggggtta
    23341 agattagtca gacagctggt tacatcgaag ccaaggcaga acgcttgcat ggcgctcata
    23401 tctatatgga ctttccaagt gttggtgcaa cgcagaactt gatgatggca gcgactctgg
    23461 ctgatggggt gacagtgatt gagaatgctg cgcgtgagcc tgagattgtt gacttagcca
    23521 ttctccttaa tgaaatggga gccaaggtca aaggtgctgg tacagagact ataaccatta
    23581 ctggtgttga gaaacttcat ggtacgactc acaatgtagt ccaagaccgt atcgaagcag
    23641 gaacctttat ggtagctgct gccatgactg gtggtgatgt cttgattcga gacgctgtct
    23701 gggagcacaa ccgtcccttg attgccaagt tacttgaaat gggtgttgaa gtaattgaag
    23761 aagacgaagg aattcgtgtt cgttctcaac tagaaaatct aaaagctgtt catgtgaaaa
    23821 ccttgcccca cccaggattt ccaacagata tgcaggctca atttacagcc ttgatgacag
    23881 ttgcaaaagg cgaatcaacc atggtggaga cagttttcga aaatcgtttc caacacctag
    23941 aagagatgcg ccgcatgggc ttgcattctg agattatccg tgatacagct cgtattgttg
    24001 gtggacagcc tttgcaggga gcagaagttc tttcaactga ccttcgtgcc agtgcagcct
    24061 tgattttgac aggtttggta gcacagggag aaactgtggt cggtaaattg gttcacttgg
    24121 atagaggtta ctacggtttc catgagaagt tggcgcagct aggtgctaag attcagcgga
    24181 ttgaggcaag tgatgaagat gaataagaaa tcaagctacg tagtcaagcg tttactttta
    24241 gtcatcatag tactgatttt aggtactctg gctctaggaa tcggtttaat ggtaggttat
    24301 ggaatcttgg gcaagggtca agatccatgg gctatcctgt ctccagcaaa atggcaggaa
    24361 ttgattcata aatttacagg aaattaggct ggagaaccag cctttttcta aagataagga
    24421 gaaatatgaa caaaaaaaca agacagacac taatcggact gctagtgtta ttgcttttgt
    24481 ctacagggag ctattatatc aagcagatgc cgtcggcacc taatagtccc aaaaccaatc
    24541 ttagtcagaa aaaacaagcg tctgaagctc ctagtcaagc attggcagag agtgtcttaa
    24601 cagacgcagt caagagtcaa ataaagggga gtctggagtg gaatggctca ggtgctttta
    24661 tcgtcaatgg taataaaaca aatctagatg ccaaggtttc aagtaagccc tacgctgaca
    24721 ataaaacaaa gacagtgggc aaggaaactg ttccaaccgt agctaatgcc ctcttgtcta
    24781 aggccactcg tcagtacaag aatcgtaaag aaactgggaa tggttcaact tcttggactc
    24841 ctccaggttg gcatcaggtc aagaatctaa agggctctta tacccatgca gtcgatagag
    24901 gtcatttgtt aggctatgcc ttaatcggtg gtttggatgg ttttgatgcc tcaacaagca
    24961 atcctaaaaa cattgctgtt cagacagcct gggcaaatca ggcacaagcc gagtattcga
    25021 ctggtcaaaa ctactatgaa agcaaggtgc gtaaagcctt ggaccaaaac aagcgtgtcc
    25081 gttaccgtgt aaccctttac tacgcttcaa acgaggattt agttccctca gcttcacaga
    25141 ttgaagccaa gtcttcggat ggagaattgg aattcaatgt tctagttccc aatgttcaaa
    25201 agggacttca actggattac cgaactggag aagtaactgt aactcagtaa aagataagcc
    25261 taaactccta tgtcacttat ggatgtagga gttcttttta ctagtttaag cagggctaga
    25321 acaggtacta agaaaaaata gcaacttcta aaactaactt ccagttttgg gagagagatg
    25381 gaagttactt tgagaagtta cggtaagaaa aaataatatg tattggatta aattttgtga
    25441 tataataaag gataagatac tattttagga gaagaactat ggaagaccca agtagtcaga
    25501 atttgttgct acaatttgtt ttattgttta tcttgacggt attaaatgcc tttttctcag
    25561 ccacagaaat ggccatggtt tctcttaatc gtgcccgagt tgaacaaaag gcggaagaag
    25621 gagacagacg ctacatccgc ctgctgaagg tactagaaaa tcctaaccac tttttatcaa
    25681 ccattcaggt aggaatcacc ctgattacaa tcttatcagg ggcgagttta actaatactc
    25741 taggacgtgt gattgcctct tggcttggga atggcgaaac agcacaagcc gtggcaactt
    25801 ttctatcctt ggcatttttg acctatattt ccatcgtttt tggggaatta tatcctaaga
    25861 gaatcgctct taatctaaag gatgccttgg caattcgtat agcgccggtt atcattggga
    25921 tcggaaaact agtcagtcct tttgtttggc ttttagctgc ttcgaccaat ttcttgagcc
    25981 atttgactcc tatgtcattc gatgatgcgg atgaaaagat gacccgtgat gaaattgcct
    26041 atatgctgac aaatagtgaa gaaacattgg atgctgatga gattgagatg ctacaaggtg
    26101 tcttttcgct cgatgaactg atggcacgag aggttatggt tcctcgaacg gatgccttta
    26161 tggtggatat tcaggatgat agtcaagcca ttatccaaag tattttaaaa caaaattatt
    26221 ctcgtatccc ggtttatgat ggggataagg acaatgtaat tggaatcatt cacaccaaga
    26281 gtctccttaa ggcaggcttt gtggacggtt ttgacaatat tgtttggaag agaattttgc
    26341 aagatccact ttttgtacct gaaactattt ttgtggatga cttgctaaaa gaactgcgaa
    26401 atacccaaag acaaatggca atcttgctgg atgaatacgg tggtatggct ggtttggtga
    26461 ctttggaaga cctcttagag gaaattgttg gtgaaattga tgatgaaacg gacaaagcag
    26521 caatagatgt tcatcagatt ggcgaggata cctacattgt tcaaggaact atgaccctca
    26581 atgatttcaa taattacttt gatgttgaac tggaaagtga tgacgttgat accatcgctg
    26641 gttattattt gacaggagtg ggaactattc caacgactga gaaactcagc tatgaattgg
    26701 ttagccaaaa caaacagttt atcttaacca atgataaagt gaagaatgga cgtgttacca
    26761 aggtaaaagt ccaaatcaca gaagtcgaaa tagaagaaga aacagaataa actagaggct
    26821 tttgtcacca ggcgtgacga agcttttttc aattgatttc aaaagggaat tatgccatgg
    26881 aaagaactct gccacaaact cgctccaaaa gtttttaaag ttatacgtat ttattcgagg
    26941 gaaaataaaa aaagcccttc aaattgggct ttctgttcat ttgaaacctg ataaatcaag
    27001 ttatagaagg cggtagacgg atttgaaccg acgatcaagc ttttgcagag ccgtgcctta
    27061 ccacttggct ataccgcctt aacgtttatt attatacctt gaaaatgatt ttcagtcaat
    27121 agttttttca gttttttgta ccatagcgtg attcgaatgt gatgccaagt tttctatggg
    27181 aaaaaattta ccaaaaacct actaaaaccc cttgaaaaat ctagctaaat agggtataat
    27241 atgggtaatc atttgtcgta ggttttgtct gaaatattgt ccagacaagg ctcacagcag
    27301 ttaaatcttc tgaaaaagtc agatttagct gctctttttg tgcttttttt caagattttg
    27361 agcatttgta acagaggctt aaagattctg aaaattcgtc aagaggacac ggtgataagg
    27421 ggtttacaac catatgacga ttagaaaagc ctgattgaca aggcttggaa cttatttaca
    27481 aaggagaatc atcttggcag gacatgacgt tcaatacggg aaacatcgta cccgtcgtag
    27541 tttttcaaga atcaaagaag ttcttgactt accaaatttg attgaaattc aaactgactc
    27601 attcaaagct ttcctagacc acggtcttaa ggaagtgttt gaagatgtat tgccaatttc
    27661 aaacttcaca gacacaatgg agttggaatt tgttggatat gaaatcaagg aaccaaaata
    27721 tacgctagaa gaagctcgta tccacgatgc tagctactca gcaccaattt ttgtaacctt
    27781 ccgtttgatc aataaagaaa caagcgaaat caagacccaa gaagttttct ttggtgattt
    27841 cccaatcatg acagaaatgg gtactttcat catcaatggt ggtgaacgta ttatcgtttc
    27901 tcagttggtc cgctcaccag gtgtttactt taacgacaaa gtagacaaaa atggtaaggt
    27961 gggctatggt tcaactgtta tccctaaccg tggagcttgg ttggaacttg aaagcgactc
    28021 aaaagatatc acctacactc gtatcgaccg tactcgtaag attccattta caaccttggt
    28081 tcgtgctctt ggtttctcag gtgatgatga aatctttgat atctttggtg acagcgaatt
    28141 ggttcgcaac actgttgaaa aagatatcca caagaatcca atggactctc gtacagacga
    28201 agccttgaaa gaaatttacg aacgccttcg tccaggtgag cctaagacgg ctgaaagctc
    28261 acgtagcttg cttgtagctc gcttctttga cccacgtcgt tatgacttgg cagcagttgg
    28321 tcgttacaaa atcaataaaa aactcaatgt taaaacacgt ttactcaacc aaaccattgc
    28381 agagccattg gtagaccctg aaactggaga aatcttggta gaagctggta cgattatgac
    28441 tcgtagcgtg attgaaagca ttgaaagcca tttggatggc gacttgaaca agattgtcta
    28501 catcccaaac gatgcagccg ttgtgactga gcctgttgtt cttcaaaaat tcaaggttat
    28561 tgctccaact gatccagatc gcgtcgtaac gatcattggt aatgctaacc cagatgacaa
    28621 ggttcgtacg gtgactcctg cagatatcct tgctgagatg agctacttcc tcaacttggc
    28681 tgaaggactt ggccgtgtag atgatatcga ccaccttgga aatcgtcgta tccgtgcggt
    28741 tggtgaattg cttgccaacc aagtacgttt gggactttct cgtatggaac gtaatgtccg
    28801 tgaacgtatg tctgttcagg acaatgaagt cttgacacca caacaaatta tcaatattcg
    28861 tcctgtaaca gctgcagtta aagaattctt tggttcatca cagttgtcac agttcatgga
    28921 ccaacacaac ccgctttctg agttgtctca caaacgccgt ttgtcagcct taggacctgg
    28981 tggtttgact cgtgaccgtg ctggatatga agtgcgtgac gtgcactaca ctcactatgg
    29041 tcgtatgtgt ccaatcgaga cacctgaagg acctaacatc ggtttgatca ataacttgtc
    29101 atcttacgga cacttgaaca aatatggttt tgttcaaaca ccataccgta aggttgaccg
    29161 tgaaacaggt gttgtcacga acgaaattgt ttggttgaca gctgatgaag aagatgaata
    29221 tactgtagct caggctaact ctcgtctgaa tgaagatgga acctttgctg agaagattgt
    29281 catgggacgt caccaagggg tcaaccaaga gtatccagct aatattgttg actacatgga
    29341 cgtttcacca aaacaggtag ttgccgttgc gacagcatgt attcctttct tggaaaacga
    29401 tgactccaac cgtgccctca tgggagccaa tatgcaacgt caggctgtgc cactgattaa
    29461 tcctcaggca ccttacgttg gtactggtat ggaataccaa gcagcccacg attctggtgc
    29521 ggctgtgatt gctcagtatg atggtaaagt tacttacgca gatgctgaca aggtagaagt
    29581 tcgtcgtgaa gatggttcat tggatgttta ccacatccaa aaattccgtc gttcaaactc
    29641 aggtactgct tacaaccaac gcactctcgt aaaagttggt gatgtcgttg aaaaaggcga
    29701 tttcatcgct gacggacctt ctatggaaaa tggagaaatg gcgcttggac aaaacccaat
    29761 cgttgcctac atgacttggg aaggttacaa cttcgaggat gccgttatca tgagcgaacg
    29821 cttggtgaag gacgatgtct acacatctgt tcaccttgaa gaatacgaat cagaaacgcg
    29881 cgatacaaag cttgggcctg aagaaatcac tcgcgaaatt ccaaacgttg gtgaagatgc
    29941 cctcaaagac cttgacgaaa tggggattat ccgtattggt gctgaggtta aagaaggtga
    30001 tattcttgta ggtaaagtaa cacctaaggg tgagaaagat ctttcagctg aagaacgtct
    30061 cttgcacgct atctttggag acaagtctcg tgaagtgcgt gatacttctc ttcgtgtacc
    30121 acacggtgcc gatggtgtcg ttcgtgatgt taagatcttt acacgtgtaa atggagatga
    30181 gttgcaatca ggtgttaaca tgttggttcg tgtttacatc gctcaaaaac gtaagattaa
    30241 ggtcggagat aaaatggccg gacgtcacgg aaacaaaggg gttgtctctc gtatcgttcc
    30301 tgtagaagac atgccttacc ttccagacgg aactccagtc gacatcatgt tgaacccact
    30361 tggggtacca tcacgtatga atatcggtca ggttatggag cttcaccttg gtatggcagc
    30421 tcgtactctt ggtattcaca tcgctacacc agtctttgat ggagcaagtt ctgaagatct
    30481 ttggtcaact gttaaagaag caggtatgga tagcgatgcc aaaacgatcc tttacgatgg
    30541 acgtactggt gaaccgtttg ataaccgtgt ttctgtcgga gtcatgtaca tgatcaaact
    30601 ccaccacatg gttgatgata aattgcacgc gcgttcagtc ggaccatact caactgttac
    30661 ccaacaacca ctcggaggta aggctcagtt tggtggacaa cgtttcggtg agatggaggt
    30721 ttgggctctt gaagcctacg gtgcgtcaaa tgtccttcaa gaaatcttga cttacaagtc
    30781 ggacgatatc aacggacgtt tgaaagccta tgaagctatt acaaaaggca aaccaattcc
    30841 aaaaccaggt gttccagaat ccttccgagt tcttgtcaaa gaattgcaat ctcttggtct
    30901 tgacatgcgt gtcctagacg aagatgacca agaagtggaa cttcgcgact tggatgaagg
    30961 aatggacgaa gatgtcatcc acgtagatga ccttgaaaaa gcccgcgaaa aagcagccca
    31021 agaggctaaa gcagcctttg aagctgaaga agctgagaaa gcaacaaaag cggaagcaac
    31081 agaagaagct gctgaacaag aataagcagt tcacttagaa tagaaaggga agaaatagtg
    31141 gttgatgtaa atcgttttaa aagtatgcaa atcaccctag cttctccaag taaagtccgt
    31201 tcatggtctt atggagaagt caaaaaacct gaaacaatca attatcgtac cttgaaacca
    31261 gaacgtgaag gactctttga tgaagtgatc tttggtccta caaaagactg ggaatgtgct
    31321 tgtggtaagt acaaacgcat tcgttacaga ggaattgttt gtgaccgctg tggggttgaa
    31381 gtaacgcgta cgaaagttcg tcgtgagcgt atgggacata tcgaattgaa agctcctgta
    31441 tctcacatct ggtacttcaa ggggattcca agccgtatgg gcttgaccct tgatatgagc
    31501 cctcgtgccc tcgaggaagt tatctacttt gcggcttatg tggtgattga tcctaaggat
    31561 acaccacttg agcacaagtc tatcatgaca gagcgcgaat accgagagcg cttgcgcgaa
    31621 tatggttatg gttcatttgt tgctaagatg ggtgcggaag ccatccaaga ccttttgaag
    31681 caagtagatc ttgaaaaaga aattgctgaa ctcaaagaag aattgaaaac tgctactgga
    31741 caaaaacgtg tcaaagccat ccgtcgtttg gatgttttgg atgcctttta caagtctgga
    31801 aacaaacctg aatggatgat tcttaacatc cttccggtta tcccaccaga tcttcgtcca
    31861 atgttgcagt tggatggtgg ccgttttgcc tcatctgact tgaatgacct ttaccgccgt
    31921 gttatcaacc gtaacaaccg tttggctcgt ttgcttgagt taaatgcacc aggtatcatc
    31981 gttcaaaatg agaagcgtat gcttcaagaa gcagttgacg ctttgattga caatggtcgt
    32041 cgtggtcgtc caatcacagg accaggtagc cgtccattga aatcattgag ccacatgctt
    32101 aaaggtaaac aaggacgctt ccgtcaaaac ttgctcggta aacgtgttga cttctcagga
    32161 cgttccgtta tcgccgttgg tccaactctt aagatgtacc aatgtggtgt gccacgtgaa
    32221 atggcgattg aactctttaa accatttgtc atgcgtgaaa tcgttgcccg tgatatcgtg
    32281 caaaacgtca aagcagctaa acgcttggtg gaacgcggag atgagcgtat ctgggatatc
    32341 cttgaagaag tgattaaaga acacccagtg cttttgaacc gcgcaccgac ccttcaccgt
    32401 ttgggtatcc aagccttcga gccagtcttg attgatggta aggctcttcg cttgcaccca
    32461 cttgtctgtg aagcctacaa tgctgacttt gacggggacc aaatggccat ccacgtacca
    32521 ctttcagaag aagcacaagc agaagctcgt atcctcatgc tagctgctga gcacatcttg
    32581 aacccgaaag atgggaaacc ggtagttact ccatctcagg acatggtttt gggtaactac
    32641 tacttgacca tggaagaagc tggtcgcgaa ggtgaaggaa tggtcttcaa agaccgtgac
    32701 gaagcggtta tggcttaccg caatggttat gttcacctcc actcacgtgt tggtatcgca
    32761 acagacagcc tcaacaagcc ttggacagaa gagcaaagac ataaggtctt gcttacaaca
    32821 gttggtaaaa ttctcttcaa cgatatcatg ccagaggggc taccatactt gcaagaacca
    32881 aacaatgcca acttgacaga aggtgttcca gctaaatact tcttgccact tggtggagct
    32941 atcaaggaag ctatcagcaa tcttgagctc aaccctccat tcaagaagaa aaacctcgga
    33001 aatatcatcg ctgaaatctt caaacgtttc cgtacgacag aaacttctgc cctacttgac
    33061 cgcatgaaga acctcggtta ccaccactca actcttgcag gattgacagt gggtattgcc
    33121 gatatcccag tcgttgatga caaggctgaa atcattgaag aatcacacaa acgtgtcgaa
    33181 caaatcacca aacaattccg tcgtggtatg atcacagacg acgagcgtta caatgctgtt
    33241 acagctgaat ggcgtgctgc ccgtgaaaaa cttgagaaac gcttgattgc taatcaagat
    33301 cctaagaacc caatcgttat gatgatggac tctggagccc gtggtaacat ctcaaacttc
    33361 tcacagcttg ccggtatgcg tggtctgatg gctgctccga atggacgtat catggaattg
    33421 ccaatccttt caaacttccg cgaaggtttg tcggtattgg aaatgttctt ctcaactcac
    33481 ggtgctcgta aaggtatgac cgatacggcc cttaagacag ccgactcagg ttacttgact
    33541 cgtcgtttgg tcgacgttgc ccaagacgtt atcatccgtg aggacgactg tggaactgac
    33601 cgtggtctct tgatccgttc tatcgcagaa ggaaaagaga tgatcgagtc tctcgaagag
    33661 cgtctcaacg gtcgttacac taagaaaact gttaaacatc cagaaactgg tgcagtgatt
    33721 attggtccaa atgaattgat tacagaagac aaggcgcgtg aaattgtcaa tgctggtgtg
    33781 gaagaagtga ctatccgttc tgtatttaca tgtaacactc gtcacggtgt ctgccgtcac
    33841 tgttacggta tcaacttggc gactggtgat gcggttgaag ttggtgaagc agttggtaca
    33901 atcgctgccc aatctatcgg ggaacctggt acacagctta caatgcgtac cttccacaca
    33961 ggtggggttg cctcaaatac cgatatcact cagggtcttc ctcgtgtcca agaaatcttt
    34021 gaagcccgca atcctaaagg ggaagcggtt attacagagg ttaaaggaca agttactgct
    34081 atcgaagaag atgcatcaac tcgtaccaag aaagtctttg ttaagggtga aactggcgaa
    34141 ggtgaatatg tcgttccatt tacagctcgt atgcgtgtcg aagttggggg ccaagtagcg
    34201 cgtggtgctg ctctgacaga aggttctatc caaccaaaac gtctccttgc agttcgtgat
    34261 gtcttgtcag ttgaaacgta ccttctcggt gaagtacaaa aagtttaccg tagccaaggg
    34321 gtagaaatcg gtgacaaaca catcgaggta atggttcgtc aaatgatccg taaagtccgt
    34381 gtcatggatc caggtgatac agatcttctc atgggtaccc tcatggatat caatgacttt
    34441 acagatgcca acaaagatgt ccttatcgca ggtggagttc cagcgacagg tcgcccagtc
    34501 cttatgggaa ttaccaaagc ctcacttgaa acaaacagtt tcttgtcagc ggcttccttc
    34561 caggaaacaa ctcgtgtcct tactgacgca gctatccgtg gtaagaaaga ccatctcctt
    34621 ggacttaaag aaaatgttat catcggtaag atcatcccag ctggtactgg tatggcccgt
    34681 taccgtaacc ttgaaccaca tgctgtcaac gaagaagaat accttaatcc tccagtagag
    34741 gaagaaggaa atgaagaaac aacagaagta gttgtggata ctgccgttga aactgtggaa
    34801 gaaacagtag aataagagag aatgagagaa cccgagggtt ctctcattct ctcttctgaa
    34861 aactttctcc atttttccac gaaatgtggt aaaataaaag aaagaagctg acaaaggaga
    34921 cgggtatgga acaaacattc tttatcatca aaccagatgg tgtaaaaaga gggctagtgg
    34981 gtgaagtgtt aaagcgcatc gaacaacgtg gatttacaat cgaaaaattg gagtttcgtt
    35041 cacaggtttc agaagagttg attgaccagc actatcagga cttggttggt cagagttttt
    35101 acccaccgat tcgtgaattc atgacttcag gtccagttct tgtgggtgtc atttctggtc
    35161 ccaaagtaat cgaaacttgg cggaccatga tgggtgcaac tcgtccagaa gaagctttac
    35221 caggcactat tcgaggtgat tttgcaaaag ctgctggaga aaatgagatt atccaaaatg
    35281 ttgtacatgg ttcagattca gaagagtcag ctaagcgaga aattgctctt tggttttaag
    35341 agtggattgg ctcaatcaat tggttaaaag ctcatttgaa tagaaagtat agtcaattag
    35401 tttaagacat gacgcatgat atcaaacttt ttagtttttg atatggtgcg ttttttgatt
    35461 aagtgactat tagtttgtct cgctccgtta ggtgcgagac aaaaaaaaca cccgctatgc
    35521 gggtgtgcgt cgaaggttat accaaaaaaa ctccaaacgc gatacaataa aggtgttcaa
    35581 tccaattgta aagcgaaagg agaaaaatat ggcacaaaag gttcttttat cctgttcttg
    35641 tttcaattga ctatatttct atactttttc ttcctttctc tgtgaaaatc gcaagttttt
    35701 cccacctttt ctcaatactg aaacaacatc tttccagaac ttcaataatt tctttttgtg
    35761 ttaaatcatt atttaactaa atctttggag gcaatacgtt ttcaaagcta cttttttacc
    35821 ataggttttg agccttcaac acagattact aagccaaaaa tgctctaaat ttttgaaatt
    35881 ctaacaaatt ccactcaact ttatcatcat ctttgacttt cacaatatct tcctcaaaat
    35941 aacatggaat aaagatagtt ttcttttaaa acggttgtag gaggaaaatc tctcctacac
    36001 tattattgat tctgagagaa aattgcaaaa tatcatctac caaactttca attattaaga
    36061 ttttttgcag tttatctgaa ttagaatcgc aaggcttctc aataatataa ttgtcgaatt
    36121 ttttcattct aacttcctct ccattatttc ttcctttatc aagaaaaagt tacctcaata
    36181 ttaataaagt acaattctaa attaactcca attcttgatt catgataata tttcagtaac
    36241 tcatcgtgca attaaaaaca aggattttta tttcagatga tccatcgtga acacttcatc
    36301 agccatctcc caaattgccg gattatgtgt tgcgataatg attagcctat tatcatctcg
    36361 aagagatagc aaaatctcca taatcaactg agaggttgct gggtctattg aagctgttgg
    36421 ctcatctgcc agaataaagg gtggattctt taagataatt tttgccaagg caacccgttg
    36481 cgattctccg cccgataact caaagatgcg cttatctagg tcaagataaa ccaggccgac
    36541 ctgttctaaa gcctgcttct gcctcaaccg ctgttccgac cgactcaact tttgaccaat
    36601 gagacctagc ttaaggtttt cttcaatact ttggttttca attaagccaa agttctggaa
    36661 gaggtagccc aattcgtgac ggaaaaaatc acttgattta taattggcca agtctttacc
    36721 tcggtaaaaa atcgtcccat cataaggttc taatttccca atcatgttca tcaaggttgt
    36781 ttttccgcta ccacttgaac caattaaggc atagactttt ccagcctcaa atgtcattga
    36841 aagattcgaa aataactctc gttctccaaa agatttactc acctgtttaa gttcaatcat
    36901 attaacctcc cttcaaaaca agcatggaca tcttgttttc tttctgcatt tggacatgta
    36961 actgtaaaag agatagacca gtaaagagta acaagactaa gaaagcaact cctatctcta
    37021 cctgaagaaa tacactcgca acaaatccca gtaaaaacac acccagttga gcaaagagat
    37081 aagtgcgatg gatttctaag aacctgagac ctgcaatgcg tttgataaaa atggcacgtc
    37141 taaattcttc aaagtagagc ctattcatag tgttaaacaa caagattgaa gttgcaatcc
    37201 caagcacagc tcctgctagc attacccaac gttccctctg gatattatcc aataatgtga
    37261 tgtagttgtg gtaacctgtt tgcatttctg agacccaatt ttcaatgcct tgtctctgga
    37321 taagctcctg ggcatcagac aattgattaa agagaacgta gttctgtact gcgtctatcc
    37381 aaaacaaaat ggactgtgga ccagttgatt ggggtgttat aacaatgatg attggatctt
    37441 tcaaaaactg ctggtaagaa ataggggtcg tattatacac aaaacgatcc tgacctgatt
    37501 ctaaataacc taccgtagca gtcatttgct gtcgttcatc ttgactagat atgcgactgg
    37561 ttaagtcgtc ttcaaaaaca gatttataat gttcttcctc tgaacggagg tgttcaggca
    37621 gcaataagac aaactcccct acatcaaggt gattcatctt ttgactcatt ttagttgaac
    37681 tgtaggactt actttttcag agggctccaa attttatttt catttacttt ttttagccca
    37741 taaaatattt accatactta taaaaagtag taatactcca actaaactga cttgtaatga
    37801 attatatgta ttttctgaag tgttaggtaa ataattttga gagtaccctt ctcgggcaat
    37861 agtaataata ttgtgtgtgt ttatttgttt aattccaata gtagtttgaa ttctctcttc
    37921 caactcaatt aaattaacat tatctatgaa actatcccaa ctttgttcat ttttatattc
    37981 taattgtaac atattcaaat catatctcaa tacgtttggt atatttaaca cttttatacc
    38041 attttttact ggtaatgttt cttccagaat tttttttaca aacatataat tatagggctg
    38101 atcttttaat ttgtatttat caataatcat tgcaatcgca ccagaaactt taggagcagc
    38161 aaatgagttt ccataaagat acgtatatcc tcctagtgtt gccgaaaaaa tccaatcttc
    38221 tttatatttt tctgcattaa taaagttatt aagtcctaat tctgaaagag ataatgttga
    38281 gcctgcaggc gcatatattg catcagaatc ccctttattg ctaaaatctg ataaattacc
    38341 taagcgatct atgcctccga ccgaaattgt agatgagaaa taacttggaa catcaactac
    38401 taatcctggt tttcttactt ttttgcgtga actaataagt ttcaataaat ctgactgatt
    38461 tgatacattt agggagtcat tccctaatgc agctactata attactcctt tttgattcgc
    38521 gtaatcaata gcctttttat acttcaaaaa tgtttcaaaa tcatttgttc cgtcctcata
    38581 ttctccatca atcattaaat attgtccagt actaagattg ataatatcat tgccatcatc
    38641 tacagcatca aaaattgctt ttacaatcca atctggacta gctgacgact taccaaatat
    38701 tctatagacg ttaatattaa cgtgaggatt tactccatta atattgtcat cccctacaat
    38761 ttgagctaca acagccgtac catgacctaa tctatcttga atatctgata ttattccttc
    38821 ctctccctcc tcttttccta aatatccttt attggggaca tagttttttg agtgatttga
    38881 aagatttttc agtaatccag tattttgtaa atcgactcct gaatctaaaa tagcaattga
    38941 aactttagaa ttacttttag aatataattt ataactttcg cctccaccag taactttatc
    39001 tatatcccat tcatattttc ttacaatcga cgattctaag ttatctttgt catgctctat
    39061 atttcctcct ctattatcta gatgagaaac attttcctta attttattat ctaaattatc
    39121 ttcgcctagt aaattattac tttcagagtt aacaatatcg ttatttattt gt
//
LOCUS       DAVKFH010000015        35195 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKFH010000015 DAVKFH010000000
VERSION     DAVKFH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.015"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..83)
                     /locus_tag="VW006_000809"
     CDS             complement(<1..83)
                     /locus_tag="VW006_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEU9379691.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEE"
     gene            78..332
                     /locus_tag="VW006_000810"
     CDS             78..332
                     /locus_tag="VW006_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9379692.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            578..1912
                     /gene="trmFO"
                     /locus_tag="VW006_000811"
     CDS             578..1912
                     /gene="trmFO"
                     /locus_tag="VW006_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9379693.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1996..2733
                     /locus_tag="VW006_000812"
     CDS             1996..2733
                     /locus_tag="VW006_000812"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9379694.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2742..3299
                     /gene="frr"
                     /locus_tag="VW006_000813"
     CDS             2742..3299
                     /gene="frr"
                     /locus_tag="VW006_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9379695.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3359..4213
                     /gene="cvfB"
                     /locus_tag="VW006_000814"
     CDS             3359..4213
                     /gene="cvfB"
                     /locus_tag="VW006_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9379696.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4222..4437
                     /locus_tag="VW006_000815"
     CDS             4222..4437
                     /locus_tag="VW006_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9379697.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4523..5491
                     /locus_tag="VW006_000816"
     CDS             4523..5491
                     /locus_tag="VW006_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9379698.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5469..5648)
                     /locus_tag="VW006_000817"
     CDS             complement(5469..5648)
                     /locus_tag="VW006_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379699.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            5684..6184
                     /locus_tag="VW006_000818"
     CDS             5684..6184
                     /locus_tag="VW006_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379700.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6187..6447
                     /locus_tag="VW006_000819"
                     /pseudo
     CDS             6187..6447
                     /locus_tag="VW006_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6814..7140)
                     /locus_tag="VW006_000820"
     CDS             complement(6814..7140)
                     /locus_tag="VW006_000820"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379701.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7086..7499)
                     /locus_tag="VW006_000821"
     CDS             complement(7086..7499)
                     /locus_tag="VW006_000821"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9379702.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(7500..7925)
                     /locus_tag="VW006_000822"
     CDS             complement(7500..7925)
                     /locus_tag="VW006_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9379703.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8102..8671
                     /locus_tag="VW006_000823"
     CDS             8102..8671
                     /locus_tag="VW006_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379704.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8739..9389
                     /locus_tag="VW006_000824"
     CDS             8739..9389
                     /locus_tag="VW006_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU9379705.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            9373..11613
                     /locus_tag="VW006_000825"
     CDS             9373..11613
                     /locus_tag="VW006_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9379706.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11755..11979
                     /locus_tag="VW006_000826"
     CDS             11755..11979
                     /locus_tag="VW006_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379707.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYDSYSEYPVGSGWNYGRYEIINYYSGGY"
     gene            12012..12599
                     /locus_tag="VW006_000827"
     CDS             12012..12599
                     /locus_tag="VW006_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379708.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            12603..13784
                     /locus_tag="VW006_000828"
     CDS             12603..13784
                     /locus_tag="VW006_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379709.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    13944..14075
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14091..14621
                     /gene="infC"
                     /locus_tag="VW006_000829"
     CDS             14091..14621
                     /gene="infC"
                     /locus_tag="VW006_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9379710.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14654..14854
                     /gene="rpmI"
                     /locus_tag="VW006_000830"
     CDS             14654..14854
                     /gene="rpmI"
                     /locus_tag="VW006_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9379711.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14906..15265
                     /gene="rplT"
                     /locus_tag="VW006_000831"
     CDS             14906..15265
                     /gene="rplT"
                     /locus_tag="VW006_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9379712.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15323..15703
                     /locus_tag="VW006_000832"
     CDS             15323..15703
                     /locus_tag="VW006_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9379713.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15953..16753
                     /locus_tag="VW006_000833"
     CDS             15953..16753
                     /locus_tag="VW006_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9379714.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16764..17702
                     /locus_tag="VW006_000834"
     CDS             16764..17702
                     /locus_tag="VW006_000834"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9379715.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17744..19645
                     /locus_tag="VW006_000835"
     CDS             17744..19645
                     /locus_tag="VW006_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9379716.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(19706..21361)
                     /gene="pavA"
                     /locus_tag="VW006_000836"
     CDS             complement(19706..21361)
                     /gene="pavA"
                     /locus_tag="VW006_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9379717.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIGLDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21466..21963
                     /gene="ybeY"
                     /locus_tag="VW006_000837"
     CDS             21466..21963
                     /gene="ybeY"
                     /locus_tag="VW006_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9379718.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHKLNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21944..22339
                     /locus_tag="VW006_000838"
     CDS             21944..22339
                     /locus_tag="VW006_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9379719.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22356..23255
                     /gene="era"
                     /locus_tag="VW006_000839"
     CDS             22356..23255
                     /gene="era"
                     /locus_tag="VW006_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9379720.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23304..24128
                     /gene="mutM"
                     /locus_tag="VW006_000840"
     CDS             23304..24128
                     /gene="mutM"
                     /locus_tag="VW006_000840"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9379721.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24128..24733
                     /locus_tag="VW006_000841"
     CDS             24128..24733
                     /locus_tag="VW006_000841"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9379722.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24720..25919
                     /locus_tag="VW006_000842"
     CDS             24720..25919
                     /locus_tag="VW006_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEU9379723.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25919..26068
                     /gene="rpmG"
                     /locus_tag="VW006_000843"
     CDS             25919..26068
                     /gene="rpmG"
                     /locus_tag="VW006_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9379724.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26108..26341
                     /gene="secG"
                     /locus_tag="VW006_000844"
     CDS             26108..26341
                     /gene="secG"
                     /locus_tag="VW006_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9379725.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26443..28797
                     /gene="rnr"
                     /locus_tag="VW006_000845"
     CDS             26443..28797
                     /gene="rnr"
                     /locus_tag="VW006_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9379726.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNH
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYVDVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28760..29227
                     /gene="smpB"
                     /locus_tag="VW006_000846"
     CDS             28760..29227
                     /gene="smpB"
                     /locus_tag="VW006_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9379727.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29243..30103
                     /gene="tehB"
                     /locus_tag="VW006_000847"
     CDS             29243..30103
                     /gene="tehB"
                     /locus_tag="VW006_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9379728.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30176..31129
                     /gene="coiA"
                     /locus_tag="VW006_000848"
     CDS             30176..31129
                     /gene="coiA"
                     /locus_tag="VW006_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9379729.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31148..32950
                     /gene="pepF"
                     /locus_tag="VW006_000849"
     CDS             31148..32950
                     /gene="pepF"
                     /locus_tag="VW006_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9379730.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32952..33665
                     /locus_tag="VW006_000850"
     CDS             32952..33665
                     /locus_tag="VW006_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9379731.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33732..34673
                     /gene="prsA"
                     /locus_tag="VW006_000851"
     CDS             33732..34673
                     /gene="prsA"
                     /locus_tag="VW006_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9379732.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34790..35131)
                     /locus_tag="VW006_000852"
                     /pseudo
     CDS             complement(34790..35131)
                     /locus_tag="VW006_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10584 a         6543 c         7920 g        10148 t
ORIGIN      
        1 atctcttcaa aagtcgtgcg ctgaacacca acaagacgct taaatcgtgc atcagttagt
       61 tgtttacttg cttcatcatt catagaacta ctataccata ttttgtttcg caggaagtct
      121 attggaaagt aagaaatatt gaagctgagg ctattagaag aaattgtgag cgtggtgcta
      181 ttttttcagg taaaataaaa tatcacgaag attcacagtt taaaggagat cactatgttg
      241 aatgttatgc tgttttagat aatacggtta tagcaagaga tagaataaca gtccctatcg
      301 atccgttatg tggaaaagat tttatagagt agcatataat tgattcttaa ctggaatact
      361 ctttatctct ttacatcaag aaaatgacta aacagggaag tttgccttct tccctttttt
      421 tgttatacta gtagaagaaa aaaatagaaa gatttgtggg agtgaaaaag tcctgtggac
      481 tttttcagcc tgagccaaga aactcgaaag ctcgtaagtc tgattggctt tttcaatgtg
      541 aatcttaact tcatactccc aaagaggtat tagtgtcgtg tctcaatctt atatcaatgt
      601 tatcggtgct ggtttggcag gttctgaagc agcctaccaa atcgcagagc gtggtattcc
      661 agttaaactt tatgaaatgc gtggtgtcaa gtcaacacct cagcacaaaa cagacaattt
      721 tgctgagttg gtttgttcca attccttgcg tggagatgct ttgacaaatg ctgttggcct
      781 ccttaaggaa gaaatgcgtc gcttgggttc tgtgattttg gaatctgctg aggctacacg
      841 tgttcctgca ggtggtgccc ttgcagtgga ccgtgatggt ttctctcaaa tggtgaccga
      901 aaaagttgcc aaccacccct tgattgaagt ggttcgtgat gaaattacag aattgccgac
      961 agatgttatt acggttatcg ctactggtcc tttgacaagt gatgccttgg ctgaaaagat
     1021 tcatgccctt aatgacggtg atggttttta tttctacgat gcggcagcgc ctattatcga
     1081 tgtcaacact atcgatatga gcaaggtcta cctcaaatca cgttatgata agggagaagc
     1141 ggcctacctc aatgccccta tgaccaagca agaatttatg gatttccatg aagctttggt
     1201 caatgcagaa gaagcaccgc ttaattcttt tgaaaaagaa aagtactttg aaggatgtat
     1261 gcctatcgaa gtcatggcca aacgtggcat taaaaccatg ctttatggtc ctatgaaacc
     1321 agttggtctt gagtatccgg acgactacac aggacctcgt gatggagaat ttaaaacacc
     1381 ttatgcggtt gtccagctcc gtcaggataa tgcagctggt agcctataca atattgttgg
     1441 tttccagacc cacctcaaat ggggagaaca aaagcgtgtc ttccaaatga ttccgggtct
     1501 tgaaaatgcg gagtttgtcc gttatggtgt catgcatcgc aattcttaca tggattcacc
     1561 aaatcttctt gagcagactt accgttccaa gaaacaacca aatctcttct ttgctggtca
     1621 gatgacgggg gtggaaggct atgttgagtc ggcggcttcg ggcttagttg cgggaattaa
     1681 cgcagctcgt ctcttcaagg aagaaagcga ggttattttc cccgagacga cagcgattgg
     1741 aagcttagct cattacatta cccatgccga cagcaaacat ttccaaccaa tgaatgtcaa
     1801 ttttgggatc atcaaggagt tggaaggcga gcgtatccgt gataagaagg ctcgttatga
     1861 aaaaattgca gagcgtgccc ttgccgactt agaggaattt ttgactgtct aatttttttg
     1921 aaagaattgc tcatgatact ataaaaatct tagaaattgt gataaaatag gtaggatgaa
     1981 agaaggagag tgaaaatggc gaatcccaag tataaacgta ttttaatcaa gttatcaggt
     2041 gaagcccttg ccggtgaacg tggcgtaggg attgatatcc aaacagttca aacaatcgca
     2101 aaagagattc aagaagttca tagcttaggt atcgaaattg cccttgttat tggtggagga
     2161 aatctttggc gtggagaacc tgcagcagaa gcaggtatgg accgtgttca ggcagattac
     2221 actggaatgc ttgggactgt tatgaatgct cttgtgatgg cagattcatt gcaacaagtt
     2281 ggggttgata cgcgtgtaca aacagctatt gctatgcaac aagtggcaga gccttatgtc
     2341 cgtggacgtg cccttcgtca ccttgaaaaa ggccgtatcg ttatctttgg tgctggaatt
     2401 ggttcaccat acttctcgac agatacaaca gcggcccttc gtgcagctga aatcgaagca
     2461 gatgccatcc tcatggctaa aaatggcgtc gatggtgtgt acaatgccga tcctaagaag
     2521 gacaagacag ccgttaagtt tgaagaattg acccaccgtg atgttatcaa caaaggtctt
     2581 cgtatcatgg actcaacagc ctcaaccctc tcaatggaca acgacattga cttggttgtc
     2641 ttcaacatga accaatcagg caacatcaaa cgtgtcgtat ttggtgaaaa tatcggaaca
     2701 acagtttcaa ataatatcga agaaaaggaa taagaaagaa tatggctaac gtaattattg
     2761 aaaaagctaa agagagaatg acccaatctc accaatcact tgctcgtgaa tttggtggta
     2821 tccgtgctgg tcgtgccaat gcaagcttgc ttgaccgtgt acatgtagaa tactatggag
     2881 tcgaaactcc tcttaaccaa atcgcttcaa ttacgattcc agaagcgcgt gttttgttgg
     2941 taacaccatt tgacaagtct tcattgaaag acatcgaacg tgccttgaac gcttctgatc
     3001 ttggtatcac accggctaat gacggttctg tgattcgctt ggttatccca gctcttacag
     3061 aagaaactcg tcgtgacctt gctaaagaag tgaagaaggt cggcgaaaat gctaaagtgg
     3121 ctgtccgcaa tatccgtcgc gatgctatgg acgaagctaa gaaacaagaa aaagcaaaag
     3181 aaatcactga agacgaattg aagactcttg aaaaagatat tcaaaaagta acagacgatg
     3241 ctgttaaaca catcgacgac atgactgcta acaaagagaa agaacttttg gaagtctaaa
     3301 aataaacaga aaaactcagt tggcattgct ggctgagttt tattcgaaag aaggaaatat
     3361 gaatacaaat cttgcaagtt ttatcgttgg actgatcatc gatgaaaatg accgttttta
     3421 ctttgtgcaa aaggatggtc aaacctatgc tcttgctaag gaagaaggtc aacatacagt
     3481 aggggatacg gtcaaaggtt ttgcatacac ggatatgaag caaaaactcc gcctgacaac
     3541 cttagaagtg actgccactc aggaccaatt tggttgggga cgtgtcacag aggttcgtaa
     3601 ggatttgggt gtctttgtgg atacaggcct tcctgacaag gaaatcgttg tgtcactcga
     3661 tattctccct gagctcaagg aactctggcc taagaagggc gaccaactct acatccgtct
     3721 tgaagtggat aagaaagacc gtatctgggg cctcttggct tatcaagaag acttccaacg
     3781 tcttgctcgt cctgcctaca acaacatgca gaaccaaaac tggccagcca ttgtttaccg
     3841 tctcaagctg tcaggaactt ttgtttacct accagaaaat aatatgcttg gttttattca
     3901 tcctagcgag cgttacgcag agccacgttt ggggcaagta ttagatgcgc gcgttattgg
     3961 tttccgtgaa gtggaccgca ctctgaacct ctccctcaaa ccacgctcct ttgaaatgtt
     4021 ggaaaacgat gctcagatga ttttgactta tttggaaagt aatggcggtt tcatgacctt
     4081 aaatgataag tcatctccag acgacatcaa ggcaaccttt ggcatttcta aaggtcagtt
     4141 caagaaagct ttaggtggtc ttatgaaggc tggtaaaatc aagcaggacc agtttgggac
     4201 agagttgatt tagggaggct tatgagaaaa tcattttaca cttggctcat gaccgagcgc
     4261 aatcctaaaa gtaacagtcc caaagcaatt ttggcagacc tcgcttttga agaggcagct
     4321 tttccaaaac atacagatga ttttgatgag gtcagtcgct ttttggagga gcatgccagt
     4381 ttctctttta acctaggaga ttttgacagc atttggcagg aatatctaga acactagcat
     4441 ttattcattg ggtttgggct agtaatttct ccatccctct gctataataa aaagaaataa
     4501 aaggattaga gaggttcttt atttgaagga acattcaata gacattcaac tgagtcatcc
     4561 agatgacctg tttcatcttt ttggttccaa tgaacgccat cttcgtttga tggaagaaga
     4621 gcttgatgtt gtgattcatg ctcgtacgga gattgtccag gttttgggag aagagtctgc
     4681 ctgtgaggaa gcccgtcaag ttattcaggc tttgatggtc ttggtaaatc gtgggatgac
     4741 cgttggtacg ccagatgtag tcactgcgat tagcatggtc aaaaatgatg aaattgacaa
     4801 gtttgtcgcc ctttacgaag aagaaattat caaagataat actgggaaac ctatccgtgt
     4861 caaaacccta gggcaaaagc tttatgtgga cagtgtcaaa cagcatgatg tgacctttgg
     4921 aattgggcca gcaggtacag ggaagacctt ccttgcagtg accttggcag tgactgccct
     4981 taaacgtggg caagtcaagc gaattatcct aactcgtcca gcggtggaag cgggagagag
     5041 tcttggattt cttccgggtg atcttaagga gaaggtggat ccttaccttc gtcctgttta
     5101 cgatgccttg tatcagattc tggggaaaga ccaaacaact cgactcatgg agcgtgaaat
     5161 tatcgagatt gcgccccttg cctatatgcg tggacggacc ttggatgatg cctttgtcat
     5221 tctcgatgag gcgcaaaata cgaccatcat gcagatgaag atgttcttga ctcgtttagg
     5281 ttttcattct aagatgattg tcaatggaga tattagtcag attgacctgc cacgtaatgt
     5341 caagtccggt ttgattgatg ctcaagagaa actcaagaac atccatcaga ttgactttgt
     5401 tcatttttca gccaaggatg tggttcgcca tccagttgtc gctcagatta tccgagccta
     5461 tgaatattct actgaagttg cacacgactg attttgagga agtttgcctg caaaagaata
     5521 tacttttgag agaggaaaat ccagttgtat aggctaaagg ttttatccaa aggtttgaga
     5581 caacgattag gcacgatgga aagaactttt atgtggctga tgacgatcag tgcatcttcc
     5641 tgtgtcataa tcacagtgca caagaaagta ggaatttgaa aagatgattg accaactatc
     5701 taagtattac agttgtagga tactaactga aaaggatatt ccaagtattt tatctttata
     5761 tgaaagtaat cctctgtatt ttcagcattg tccaccagag ccaaattttg caactgtaaa
     5821 agaggacatg ctttgtctac ctgaaggtaa agctaaggct gataagtttt ttgttggatt
     5881 ttggaatgga tctgaccttg tggctgttat agattttgtc tatgcacatc ctgatgagga
     5941 gactgttttt attggtttgt ttatggttga tcaagcctat cagaggaaag ggattggtag
     6001 tcatattgtg acagaagcac tagcttattt tgctaagaac tttcgaaagg cacgtttggc
     6061 ttatgttaag ggaaatccgc aatctcagca tttttgggaa aagcagggct ttaaatcaat
     6121 tggatgcgag gttaagcaag aactctatac ggttgttatc gctgaacaga gcctagaaga
     6181 ttagaaatgg catcaagtaa gaactatttg gaatttgttt tggaacaatt atcaggatta
     6241 gatgatgtga cttaccgttc catgatgggg gagtatattc tttacttccg cggcaagatt
     6301 attggcggca tttatgacga tcgcttttta gttaaacccg tgcaagcagt cttagataag
     6361 attgaccaat cttcttttga gtttccatac aaaggtgcca aagaaatgat ttgagtggaa
     6421 gaacttgata ataagatgtt tctataagac ctgattttag ctatgtataa ccaactgcca
     6481 acgcccaaac ctaaaaagaa aaagcaaggg tgaacgaagt aaaaaagaag tctgctaagg
     6541 ccctgtcttt gcacgggtaa aattttatat ataaaaagaa gctgggacta aagagctcag
     6601 cttcctttgg tttatataat tgtcattaca agacgaagtg gttgggcgaa actctgttga
     6661 ctttattcaa tttagagttt cttatgcaca attgagtctg gaacgaaagt ctccagttgc
     6721 aaagtataca gtacaataaa ccaacgatgt aatagctgat gacacaaagc acagtgggta
     6781 ggacttgcga agtcaccctt ttcttttcaa aatttatact aaatcattga tatcagtgta
     6841 gtcacgatta agtccttgag caactggtag gctagtcaag taaccttgat aagtggtcac
     6901 accttgacgc aagccttcat cttcagagat tgcttgtgcg aatcctttgc cagccaaagc
     6961 ttcgatataa ggaagagtga cattggttag ggcgatggtt gaagtgcggg caaccgcacc
     7021 agggatattg gcaacggcat agtggagaac accgtgtttt tcatagacgg gttcatcgtg
     7081 cgttgtcaca cggtcagctg tttcgataac gccaccttgg tcaacagcaa cgtcaacgat
     7141 acagagcctg gacgcatttg tttgaccatc tcatctgtca ccaattccgg tgcttttgca
     7201 ccagggatga gaatggctcc aatcaccaca tcagcatctc tcatacttgc ttcaatgttg
     7261 aatgaattag atataagagt ttgaatttga cttccaaaga cttcttctag aactgagaga
     7321 cgcttggcac taatatctaa aatagtcact tgagcaccaa gaccaagggc gatgcgggca
     7381 gcatgtgtac cgacgacacc atcaccgatg atagttactt ttccttttgg aacacctggt
     7441 acaccaccag gtagaacacc agagccacca gcttgcttag taaggaagtg agctccgatt
     7501 taaacagcca tacgacctgc aacctcactc ataggaacga ggagcggtag ttgtccttga
     7561 ttgtcacgaa cagtttcata ggcaattcct gttgtttttg ctgctaacat agcatctgct
     7621 aattctggag cagcggccat gtgcaagtag gtgaagagaa gaagatcgtc gcgcaagtaa
     7681 ccgtattcag aacttaaagg ttcttttact ttcacaacca actctgctgc ccaagcttca
     7741 ccagcagtag cgacaatctc agctccttgc ttttgatagt cagcatcagt aaagccagaa
     7801 ccgagaccag catttgtttc gataaggaca cgatgaccac gactaactaa gctatgaaca
     7861 cctgcaggtg tgagggcgac acggttttcg ttatttttaa tttcttttgg gattccgatt
     7921 aacattgaga taacctacct ttcaattgac ggtcttgttt tggttgtcac attccagttc
     7981 ataaatcaaa aatgtgacgg tttcattgta tatgaaaccg cttcaaaaat caagaaaaac
     8041 ttgtcatcca aattttttta tgctagacta gtgaaaatca agctctaatg gagggaaaag
     8101 tatggaatca atatttgtga aatttgccca gtatccgtct atagaaacgg agcgtttatt
     8161 gcttagacct gtaactttgg atgatgcgga agcaatgttt gactatgcct cggacaaggg
     8221 taatacacgt tacacttttc caaccaatca aagcttggaa gaaaccaaga ataacattgc
     8281 tcagttctac ttggctaatc ccttgggacg ttgggggatt gaattaaaag ataacggcaa
     8341 gtttatcgga actattgact tacacaagat tgatcctgtt cttaagaagg cagctattgg
     8401 ctacattatc aataaaaagt attggaatca aggattaacg ccagaaacca atcgtgctgt
     8461 gattgagcta gcttttgaga agataggaat gaataagtta gttgctttat acgataagga
     8521 caattctgcg tcaggaaagg tcatggagaa atcaggcatg cgtttttccc atgcagaacc
     8581 atatgcttgt atggaccagc atgaaaaagg ccgaatcgtg acaagagttc attatgtctt
     8641 gaccaaggaa gactattttg caaataaata agcagttgaa aagaaatttt tcgactgttt
     8701 tttcttcctc ttacgaataa tctaagagag gagaaaatat ggaagcaatt atcgagaaaa
     8761 tcaaagagta taaaatcatc gtcatctgta ctggtctggg cttgcttgta ggcggatttt
     8821 tcctgctaaa gccagctcca caaacacctg taaaggaaac gaatttgcag gctgaagttg
     8881 cagctgtttc caaggatttg gtatccgaaa aggaagtgaa caaggaagag aaggaagaac
     8941 cccttgaaca agatctaatc acagtagatg tcaaaggtgc tgtcaaatcg ccagggattt
     9001 atgacttgcc tgtaggtagt cgagtcaatg atgctgttca gaaggctggt ggcttgacag
     9061 agcaagcaga cagcaagtcg ctcaatctag ctcagaaagt tagtgatgag gctctggttt
     9121 acgttcctac taagggagaa gaagcagtta gtcaacagac tggttcgggg acagcttctt
     9181 caataagcaa ggaaaagaag gtcaatctca acaaggccag tctggaagaa ctcaagcagg
     9241 tcaagggact gggaggaaaa cgagctcagg acattatcga ccatcgtgag gcaaatggca
     9301 agttcaagtc agtagacgag ctcaagaagg tctctggcat tggtggcaaa acaatagaaa
     9361 agcttaaaga ctatgttaca gtggattaag aatttctcta ttcccctaat ttacctgagt
     9421 tttctattac tttggcttta ttacgctatt ttctcagcat cttatcttgc tttgttgggc
     9481 tttgtttttc tgctagtctg tctctttatc caatttccgt ggaaatctgc tggtaaagtt
     9541 ctaataattt gcggaatctt tggattttgg tttgtttttc aaaattggca acagagtcaa
     9601 gcgagtcaaa atctggcgga ttctgttgaa agggtacgga ttttgcctga tactattaag
     9661 gttaatggtg atagtctatc ctttcgtggc aagtctaacg gtcgtgcttt ccaagtctat
     9721 tataaactcc agtccgagga ggagaaagaa gcctttcaag ctttaaccga cctgcatgag
     9781 ataggactag aagggaagct ttcggagcca gaagggcaga gaaattttgg tggctttaat
     9841 taccaagcct atctgaagac tcagggaatt taccagactc tcaatatcaa aacaatccag
     9901 tcacttcaaa agattggcag ttgggatata ggagaaaact tgtccagttt acgtcgaaag
     9961 gctgtggttt ggattaagac gcactttcca gaccctatgc gcaattacat gacaggactc
    10021 ttgctgggac atctggacac cgactttgag gagatgaatg agctttattc cagtctagga
    10081 attatccacc tctttgccct atctggtatg caggtaggtt ttttcatgaa tggatttaag
    10141 aaacttctct tgcgattggg cttgacccaa gaaaagttga aatggctgac ttatcccttt
    10201 tcccttatct atgcgggact aactggattt tcagcatcgg ttattcgcag tctcttgcaa
    10261 aagctactgg ctcaacatgg ggttaagggc ttggataatt ttgccttgac ggtgcttgtc
    10321 ctctttattg tcatgccaaa ctttttcttg acagcaggag gagtcttgtc ctgcgcttat
    10381 gcttttatcc tgaccatgac cagcaaagaa ggggaggggc tcaaggctgt tactagtgaa
    10441 agtctagtca tctccttggg catattgccc attctatcct tctattttgc ggaatttcaa
    10501 cctttgtcta tccttttgac ctttgtcttt tcctttctat ttgacttgac cttcttaccg
    10561 ctcttgtcta ttttatttgt cctttccttt ctctatccag tcattcagct gaactttatc
    10621 tttgaatggt tggagggcat tattcgcttg gtgtcacagg tgacaagtag acctctggtc
    10681 tttggacaac ccaatgcatg gtttttaatc ctattgttaa tttccttggc tttggtctat
    10741 gatttgagaa aaaacattaa aaagctaacg gtattgtgct tattgattac agggctcttt
    10801 ctcctgacca agcatccact ggaaaatgaa atcaccatgc tggatgtggg gcaaggagaa
    10861 agtattttcc tacgggatgt aactgggaaa accattctca tagatgtagg tggtaaggca
    10921 gaatcttata agaaaatcga aaaatggcaa gaaaaaatga cgaccagcaa tgcccagcga
    10981 accttgattc cctatctcaa aagtcgagga gtagctaaga ttgaccagct aattttgact
    11041 aacacggaca aggagcatgt tggagatttg tcagaggtga ccaaggcttt ccatgtaggg
    11101 gagattctag tatcaaaaga cagtctgaaa cagaaggaat ttgtggcaga actacaggcg
    11161 actcaaacaa aggtgcgtag tatgacagta ggggagaact tgcccatttt tggaagtcag
    11221 ttagaagttc tatctccaag gaaaatggga gatggaggac acgatgatac cctagttctg
    11281 tatgggaaat tcttggataa gcaatttctc ttcacgggaa atttggagga gaaaggagag
    11341 aaggacttgc tgaagcacta tccagacttg aaagtaaatg ttttgaaagc tagccaacat
    11401 ggcaataaaa aatcatcaag tccagccttt ctagaaaaac tcaaaccaga gcttactctt
    11461 atctcagttg gaaagagcaa tcgaatgaaa ctcccccatc aggaaacatt gacacgactg
    11521 gaaggtatca atagcaaagt ttatcgaact gaccagcaag gagctatacg ttttaagggg
    11581 ttggatagtt ggaaaatcga aagtgttcga taggaaggat aaatgttgta gattagtgaa
    11641 ataaactaaa aatttgttgc ataataatga taaaaatggt ataatgaaaa cgtattcaat
    11701 attgaggata taaaatcatt aaaaattagc aaaaatcgtt taattagtta gtttatgatt
    11761 attggtcttt ttcagtccag tatatctgct gtgacagtca ctaaaagtta caagtatgat
    11821 tggaatacgg tttgggaata tagtaccaac tatcacgacc atcagtatgc ttggattccg
    11881 tcatggtctc gttatgacag ctattctgag tatccagttg gttctggctg gaactacggt
    11941 cgttatgaga tcataaacta ttacagcgga ggctattaat tcttaaagag tgagaaaaag
    12001 gagggctaga tatgttgcag cttactcatg tgaccttaaa aacgcgacaa gtcatcttgc
    12061 aagatgtgga tttcaccttt aaaaagggta gggtttatgg tcttcttgct atcaatggct
    12121 ctggaaagac gaccctgttc cgtgccatta gcaatttaat tcccataagt agtggaaata
    12181 tcgcagcccc tccttcttta ttttattatg agagtattga atggctggat ggaaacttaa
    12241 gtgggatgga ctaccttcgt cttatcaaaa acatctggaa gtcaggtctg aacttgaggg
    12301 atgaaatcgc ctattgggaa atgtctgact atatcagtct tcccattcgc aagtattcct
    12361 taggcatgaa gcaacgcttg gtgattgcca tgtatttcct cagtcaggcc aaatgctggc
    12421 tcatggatga gattacaaat ggcttagatg agtattatcg acagaagttt tttgataggc
    12481 tagcacaaat cgatagacaa gaacagctgg ttcttttaag ttcccactat aaggaagagt
    12541 tggttgatgt ctgcgataga gtagtaacca ttcatcaggg gcagatagaa gaggtttagt
    12601 ttatgaaaga tgttagtcta tttttattga aaaaagtttt caaaagccgc ttaaactgga
    12661 ttgtcttagc tttatttgta tctgtactcg gtgttacctt ttatttaaat agtcagactg
    12721 caaactcaca cagcttggag agcaggttgg aaagtcgcat tgcagccaac gagagggcta
    12781 tcaatgaaaa tgaagagaaa ctctcccaaa tgtctgatac cagctcggag gaataccagt
    12841 ttgctaaaaa taatttagac gtgcaaaaaa atcttttgac gcgaaagaca gaaattctaa
    12901 ctttactaaa agaagggcgc tggaaagaag cctactattt gcagtggcaa gatgaagaga
    12961 agaattatga atttgtatca aatgacccga ctgctagctc tggcttaaaa atgggggttg
    13021 accgcgaacg gaagatttac caagccctgt atcccttgaa cataaaagca catactttag
    13081 agtttccgac ccacgggatt gatcagattg tctggatttt agaggttatc atcccaagct
    13141 tgtttgtggt tgcgattatt tttatgctaa cacaactatt tgcagaaaga tatcaaaatc
    13201 atctggacac agctcactta tatcctgttt caaaagtgac atttgcaata tcctctcttg
    13261 gagttggagt gggatatgta actgtgctgt ttatcggaat ctgtggcttt tcttttctag
    13321 tgggaagtct gataagtggt tttggacagt tagattatcc ctacccgatt tatagcttag
    13381 tgaatcaaga ggtaactatt ggaaagatgc aggatgtatt atttcctggc ttgctcttag
    13441 ctttcttagc ctttatcgtc attgtggaag ttgtgtattt gattgcttac tttttcaagc
    13501 aaaaaatgcc tgtcctcttt ctttcactca ttgggattgt tggcttattg tttggcatcc
    13561 aaaccattca gcctcttcaa aagattgcac atctgattcc ctttacttac ttgcgttcag
    13621 tggagatttt atctggaaga ttacctaagc agattgataa tgtcgatcta aattggagca
    13681 tggggatggt cttacttcct tgcctgatta tccttttgtt agtggggatt ctatttatcg
    13741 aaagatgggg aagttcacgt aaaaaggaag tttttaaagt ttagttttcc tatagggaag
    13801 ctaaagaaaa actaacatag ggagagaatc aacttggttc tctctttttg attcgaaaac
    13861 caaaccaaaa tacaaacaca aacttttcaa aaaataactt tttatcttga caagagctag
    13921 aaaacttggt atcatataaa agttgagaaa agcagaagtg agagcttctc gccttgtgac
    13981 attaagttgc ctggccctac ggatgaaaag tttcgaagaa acgctatcat aacgtgcggg
    14041 cttgtatatt tacgagtccg ctattgtttt tctctaataa aacaaaagag gtgaaaacca
    14101 tagcaaagca agacttattc atcaatgatg agattcgtgt acgtgaagtt cgcttgattg
    14161 gtcttgaggg agaacagcta ggcatcaagc cactcagtga agcgcaagct ttggctgata
    14221 acgctaatgt tgacctagta ttgattcaac cccaagccaa accgcctgtt gcaaaaatta
    14281 tggactacgg taagttcaaa tttgagtacc agaagaagca aaaagaacaa cgtaaaaaac
    14341 aaagcgttgt tactgtgaaa gaagttcgtc taagtccaac aattgacaag ggtgactttg
    14401 atacaaaact tcgtaatgca cgtaagttcc ttgaaaaagg aaataaagtt aaggtatcta
    14461 ttcgctttaa gggtcgtatg attacccata aagagattgg tgcaaaagtt ttagccgagt
    14521 ttgctgaagc aactcaagat attgccatca tcgaacaacg tgctaaaatg gatggacgtc
    14581 aaatgttcat gcaattggcg ccagcgactg acaaaaaata attgtcagaa agttaaataa
    14641 aggagaaaaa atcatgccaa aacaaaaaac acaccgcgca tcagctaaac gtttcaaacg
    14701 tacaggttct ggtggactta aacgtttccg tgcttacact tctcaccgtt tccacggaaa
    14761 aactaagaaa caacgtcgtc atcttcgtaa agcatctatg gtgcattcag gagattacaa
    14821 acgtatcaaa gcaatgctta ctcgcttgaa ataatagctt gactgtaagt aaacaattct
    14881 agaaaatatt tggaggaaat aaaacatggc acgtgttaaa ggtggcgttg tatcacgcaa
    14941 acgtcgtaaa cgtattctta aattagcaaa aggttactat ggagctaaac acatcttgtt
    15001 ccgtactgca aaagaacaag taatgaactc ttactactat gcataccgtg accgtcgtca
    15061 gaaaaaacgt gacttccgca aattgtggat tactcgtatc aatgcggcgg ctcgtatgaa
    15121 cggactttca tactcacaat tgatgcatgg tttgaaattg gctgagatcg aagttaaccg
    15181 taaaatgctt gctgacttgg ctgttaacga tgcagtagct ttcacagctc ttgcagatgc
    15241 agctaaagca aaacttggta aataacaact gatgagactg gaacaggatt gtccagtctt
    15301 tttaaaaata aaggagaaag atatggcttc aaaaatgcta cacacttgct tacgagtaga
    15361 aaatcttgaa aaatcaatcg cattttatca agatgctttt ggttttaaag aattgcgtcg
    15421 cagagatttt ccagaccatg ccttcacgat tgtctatcta ggtcttgagg gtgatgatta
    15481 tgagttggag ttgacttata actacgatca cggtccgtat gtggttggtg atgggtttgc
    15541 ccatatcgcc ctcagtacac ctgatcttga ggcacttcat caagaacaca gtgcaaaagg
    15601 ctatgaagtg actgagccaa atggtctacc aggaactaca ccaaactatt actttgttaa
    15661 agaccctgat ggatataagg tcgaagtcat tcgtgaaaaa taatttcgtg caagttaagt
    15721 agaatattcg ttttctactt gacttttttt agctgaaaga gtatactaat aaaaattcaa
    15781 cctttaaggg gagtccagag agactcacaa ggtgtcagat aaaagaatag tgcaattttc
    15841 tagaggagac cttttgagtg tgctctcttg tgttgtacga ttttaactga ggccttgcac
    15901 tagcaaggtc ttttctttat ctggtcccct taaaatttaa ggaggaaaag ttatgaatct
    15961 cacatgtaag aagcgtttgg gtgtcattcg gttggaaacc atgaaggtgg ttgcacaaga
    16021 ggaaatcgcg ccagcaatct ttgaattagt cctagaagga gaaatggttg aagccatgcg
    16081 agcaggccaa tttcttcatc tgcgtgtacc agacgatgcc catctcttac gtcgtcctat
    16141 ttcaatttcg tctattgaca aggcaaacaa gcagtgtcac ctcatttatc ggattgaggg
    16201 agctgggact gcaatttttt caaccttaag tcagggagac actcttgatg tgatggggcc
    16261 tcagggaaat ggttttgact tgtctgacct tgatgagcag aatcaggttc tccttgttgg
    16321 tggtgggatt ggtgttccac ccttgcttga ggtggccaag gaattgcatg aacgtggagt
    16381 gaaagtagtg acagtcctcg gttttgctaa taaggatgct gttattttga aaacggaatt
    16441 ggctcagtat ggtcaggtct ttgtaacgac agatgatggt tcttatggca tcaagggaaa
    16501 tgtttccgtt gttatcaatg atttagacag tcagtttgat gctgtttact cgtgtggggc
    16561 tccaggaatg atgaagtata tcaatcaaac ctttgatgat cacccaagag cctatttatc
    16621 tctggaatct cgtatggctt gtgggatggg agcttgctat gcctgtgttc taaaagtacc
    16681 agaaagcgag acggtcagcc aacgcgtctg tgaagatggt cctgttttcc gcacaggaac
    16741 agttgtatta taaggagaaa attatgacta caaatcgatt acaagtttct ctacctggtt
    16801 tggatttgaa aaatccgatt attccagcat caggctgttt tggctttgga caagagtatg
    16861 ccaagtacta tgatttagac cttttaggtt ctattatgat caaggcgaca acccttgaac
    16921 cacgttttgg aaatccaact ccaagagtgg cagagacgcc tgctggtatg ctcaatgcaa
    16981 ttggcttgca aaatcctggt ttagaggttg ttttggctga aaagctacct tggctggaaa
    17041 gagaatatcc aaatcttcct attattgcca atgtagctgg tttttcaaaa caagagtatg
    17101 cagctgtttc tcatgggatt tccaaggtaa ctaatgtaaa agctatcgag ctcaatattt
    17161 cttgtcccaa tgttgaccac tgtaatcatg gacttttgat tggtcaagat ccagatttgg
    17221 cttatgatgt ggtgaaagca gctgtggaag cctcagaagt gccagtttat gtcaaattaa
    17281 ccccgagtgt gaccgatatc gttactgtcg caaaagctgc agaagatgcg ggagcaagtg
    17341 gcttgaccat gatcaatact ctggttggaa tgcgctttga cctcaaaacc agaaaaccaa
    17401 tcttggccaa tggaacaggt ggaatgtcag gtccagcagt ctttccagta gccctcaaac
    17461 tcatccgcca agtagcccaa acaacagacc tgcctatcat tggaatgggg ggagtggatt
    17521 cggctgaagc tgccctagaa atgtatctgg ctggggcatc tgctatcgga gttggaacag
    17581 ctaactttac caatccttat gcctgccctg acatcatcga aaatttacca aaagtcatgg
    17641 ataaatacgg tattagcagt ctggaagaac tccgtcagga agtaaaagag tctctgaggt
    17701 aaactgcaat caatctgttc ttgatttttt attagtttgt aatatgaatt taggagaatt
    17761 ttggtacaat aaaataaata agaacagagg aagaaggtta atgaagaaag taagatttat
    17821 ttttttagct ctgctatttt tcttagctag tccagagggt gcaatggcta gtgatggtac
    17881 ttggcaagga aaacagtatc tgaaagaaga tggcagtcaa gcagcgaatg agtgggtttt
    17941 tgatactcat tatcaatctt ggttctatat aaaagcagat gctaactatg ctgaaaatga
    18001 atggctaaag caaggtgacg actattttta cctcaaatct ggtggctata tggccaaatc
    18061 agaatgggta gaagacaagg gagcctttta ttatcttgac caagatggaa agatgaaaag
    18121 aaatgcttgg gtaggaactt cctatgttgg tgcaacaggt gccaaagtaa tagaagactg
    18181 ggtctatgat tctcaatacg atgcttggtt ttatatcaaa gcagatggac agcacgcaga
    18241 gaaagaatgg ctccaaatta aagggaagga ctattatttc aaatccggtg gttatctact
    18301 gacaagtcag tggattaatc aagcttatgt gaatgctagt ggtgccaaag tacagcaagg
    18361 ttggcttttt gacaaacaat accaatcttg gttttacatc aaagaaaatg gaaactatgc
    18421 tgataaagaa tggattttcg agaatggtca ctattattat ctaaaatccg gtggctacat
    18481 ggcagccaat gaatggattt gggataagga atcttggttt tacctcaagt ttgatgggaa
    18541 aatagctgaa aaagaatggg tctacgattc tcatagtcaa gcttggtact acttcaaatc
    18601 cggtggttac atgacagcca atgaatggat ttgggataag gaatcttggt tttacctcaa
    18661 gtctgatggc aaaatggctg aaaaagaatg ggtctacgat tctcatagtc aagcctggta
    18721 ctacttcaaa tctggtggct acatggcgaa aaatgagaca gtagatggtt atcagcttgg
    18781 aagcgatggt aaatggcttg gaggaaaaac tacaaatgaa aatgctgctt actatcaagt
    18841 agtgcctgtt acagccaatg tttatgattc agatggtgaa aagctttcct atatatcgca
    18901 aggtagtgtc gtatggctag ataaggatag aaaaagtgat gacaagcgct tggctattac
    18961 tatttctggt ttgtcaggct atatgaaaac agaagattta caagcgctag atgctagtaa
    19021 ggactttatc ccttattatg agagtgatgg ccaccgtttt tatcactatg tggctcagaa
    19081 tgctagtatc ccagtagctt ctcatctttc tgatatggaa gtaggcaaga aatattattc
    19141 ggcagatggc ctgcattttg atggttttaa gcttgagaat cccttccttt tcaaagattt
    19201 aacagaggct acaaactaca gtgctgaaga attggataag gtatttagtt tgctaaacat
    19261 taacaatagc cttttggaga acaagggcgc tacttttaag gaagccgaag aacattacca
    19321 tatcaatgcc ctttatctcc ttgcccatag tgccctagaa agtaactggg gaagaagtaa
    19381 aattgccaaa gataagaata atttctttgg cattacagcc tatgatacga ccccttacct
    19441 ttctgctaag acatttgatg atgtggataa gggaatttta ggtgcaacca agtggattaa
    19501 ggaaaattat atcgataggg gaagaacttt ccttggaaac aaggcttctg gtatgaatgt
    19561 ggaatatgct tcagaccctt attggggcga aaaaattgct agtgtgatga tgaaaatcaa
    19621 tgagaagcta ggtggcaaag attagtacta taagtgaata tgatttgagt gaatagtaag
    19681 ttaaaaatcc tgatttcaag taaaatcagg attttttcat ggatgcaatt tttttggagt
    19741 ctggtgtgac gcggagggtc ttttgtcctg tgtaagtgac aaagccgggt tttccaccag
    19801 ttggtttatt gagttttttg acttcaatca tatctacctg caccagattc gacaggcgcc
    19861 cttgagagaa gtaggcagct aactctgctg cgtctgtctt gactgcatca gatgggtcaa
    19921 gatttcctga gatgacaaca tggcttccag gaatgtcctt agcatggaac caaagttcct
    19981 ccttgcgggc cattttaaag gtcaattcct cattttgaag attgtttcgt ccgacataga
    20041 tgatggtttt gccatcgctt gctagatatt gttctagttt tttgcgtttc tggattttct
    20101 cccgttgtct tctgcggata aaacctgttt gaatcaattc ttcacggatt tcagcgattt
    20161 cttccagtcc agcttggttg aggacggttt ctacactttc cagatagaga atagtggctt
    20221 tggtttcttc aatcaaatca gtcaagtatt tgacagcttc tttgagtttc tgataccgtt
    20281 taaaatagcg ttgggcattc tggttgggag tcagagcctt atcaagccca atcatgatag
    20341 gttggttggt atagtagttg tctaggataa cctggtcttg gtcgttaggc acttggtgga
    20401 ggaaggttgt cagcaattct cctttttgac gaaattcttc agcgttgtct gtcgccagta
    20461 attctttttc ctgttttttg agtttgtgtc ggtttttctg aagttcattt tcaacacgac
    20521 gaatcagttc actggcctgc tgtttgacgc ggtcgcgctc agccttatcc ttatagtagg
    20581 tgtccaacaa atcagaaaga tttgcaaaag gctctcccac ctgatttgca aaaggaactg
    20641 gactgaagga agtctcagtc aagcatggct tggtttcctg attgaaaaaa tttcggaaag
    20701 cggaaagttt ttcactaacc agtatccttt ccaattcatt tgccgtatcg cgtcccagac
    20761 cttgaaagag gctttgaaga ttttttgctg ttagttcttg ggtttgcagg atttcaaaga
    20821 gcttttcatc cttgatagta aaaggattga gagattctgt acttggcgga gcgatatagg
    20881 tcgatcctgg aagtaaggtg cggtagctat tttgtgaaaa gccgacgtgt ttgataactt
    20941 cgaggatttt atgactgctt ttatcgacca gtagaatatt actgtgtttc cccataattt
    21001 cgataatcaa ggtagcctgg atatggtctc caatctcgtt tttattggaa actgtcattt
    21061 ccacaatacg gtcattttcc acttgctcaa tcgactcaat cagggccccc tgcaaatact
    21121 ttctcaaaac catgataaag gtagaaggtt gggctggatt ttcaaaagtc gtttgggtca
    21181 gctgaatgcg tccaaaaact ggatgggcag aaaggagcag gcgatggctt tggcgattgc
    21241 tgcggatttg caagaccaac tcttgttcaa aaggctgatt gattttctgg atgcgaccat
    21301 tcactaattc gcttcgcaat tcctcaacta tgtggtgtaa aaaaaatccg tcaaatgaca
    21361 tcgttctctc cttgtgattg tattccatag tattatatca aaaaggtaga ataaaatcat
    21421 ggaaatgtgg tataataaag ccaagtaaag agaaacgaga agcacatgta tattgaaatg
    21481 gtagatgaaa ctggtcaagt ttcaaaagaa atgttgcaac aaacccaaga aattttggaa
    21541 tttgcagcca aaaaattagg aaaagaagac aaggagatgg cagtcacttt tgtgaccaat
    21601 gagcgtagtc ataaacttaa tctggagtac cgtgacaccg accgtccgac agatgtcatc
    21661 agccttgagt ataaaccaga attggaaatt gcctttgacg aagaggattt gcttgaaaat
    21721 ccagaattgg cagagatgat gtctgagttt gatgcctata ttggggaatt gttcatctct
    21781 atcgataagg ctcatgagca ggccgaagaa tatggtcaca gctttgagcg tgagatgggc
    21841 ttcttggcag tacacggctt tttacatatt aacggctatg atcactatac tccggaagaa
    21901 gaagcggaga tgttcggttt acaagaagaa attttgacag cctatggact cacaagacaa
    21961 taaacgaaaa tggaaaaatc gtgacctgat atccagttta gaatttgcca tcacagggat
    22021 ttttactgct atcaaggaag aacgcaatat gcgaaaacat gcagtgacgg ctctagtggt
    22081 catccttgca ggttttggtt ttcaggtgtc acgaatcgaa tggctctttc tcctattgag
    22141 cattttcttg gtggtagcct ttgaaattat caattctgct attgaaaatg tggtggattt
    22201 ggccagtcac tatcactttt ccatgctggc taaaaatgcc aaggatatgg cggctggcgc
    22261 ggtattagtg gtttctcttt ttgcagcctt aacaggcgca ttgatttttc tcccacgaat
    22321 ctgggattta ttattttaaa cagtaagagg aaattatgac ttttaaatca ggctttgtag
    22381 ccattttagg acgtcccaat gttgggaagt caaccttttt aaatcacgtt atggggcaaa
    22441 agattgccat catgagtgac aaggcgcaga caacgcgcaa taaaatcatg ggaatttaca
    22501 cgactgataa ggagcaaatt gtctttatcg acacaccagg gattcacaaa cctaaaacag
    22561 ctctcggaga tttcatggtt gagtctgcct acagtaccct tcgcgaagtg gacactgttc
    22621 ttttcatggt gcctgctgat gaagcgcgtg gtaaggggga cgatatgatt atcgagcgtc
    22681 tcaaggctgc caaggttcct gtgattttgg tggtgaataa aatcgataag gtccatccag
    22741 accagctctt gtctcagatt gatgacttcc gtaatcaaat ggactttaag gaaattgttc
    22801 caatctcagc ccttcaggga aataacgtgt ctcgtctagt ggatattttg agtgaaaatc
    22861 tggatgaagg tttccaatat ttcccgtctg atcaaatcac agaccatcca gaacgtttct
    22921 tggtttcaga aatggttcgc gagaaagtct tgcacttaac tcgtgaagag attccgcatt
    22981 ctgtagcagt agttgttgac tctatgaaac gagacgaaga gacagacaag gttcacatcc
    23041 gtgcaaccat catggtcgag cgcgatagcc aaaaagggat tatcatcggt aaaggtggcg
    23101 ctatgcttaa gaaaatcggt agcatggccc gtcgtgatat cgaactcatg ctaggagaca
    23161 aggtcttcct agaaacctgg gtcaaggtca agaaaaactg gcgcgataaa aagctagatt
    23221 tggctgactt tggctataat gaaagagaat actaagtaga ggtaggctca tgcctgcttc
    23281 ttgtttttac agaaggagga cttatgcctg aattacctga ggttgaaacc gtttgtcgtg
    23341 gcttagaaaa attgattata ggaaagaaga tttcgagtat agaaattcgc taccccaaga
    23401 tgattaagac ggatttggaa gagtttcaaa gggaattgcc tagtcagatt atcgagtcaa
    23461 tgggacgtcg tggaaaatat ttgctttttt atctgacaga caaggtcttg atttcccatt
    23521 tgcggatgga gggcaagtat ttttactatc cagaccaagg acctgaacgc aagcatgccc
    23581 atgttttctt tcattttgaa gatggtggca cgcttgttta tgaggatgtt cgcaagtttg
    23641 gaaccatgga actcttggtg cctgaccttt tagacgccta ctttatttct aaaaaattag
    23701 gtcctgaacc aagcgaacaa gactttgatt tacaggtctt tcaatctgcc cttgccaagt
    23761 ccaaaaagcc tatcaaatcc catctcctag accagacctt ggtagctgga cttggcaata
    23821 tctatgtgga tgaggttctc tggcgagctc aggttcatcc agctagacct tcccagactt
    23881 tgacagcaga agaagcgact gccattcatg accagaccat tgctgttttg ggccaggctg
    23941 ttgaaaaagg tggctccacc attcggactt ataccaatgc ctttggggaa gatggaagca
    24001 tgcaggactt tcatcaggtc tatgataaga ctggtcaaga atgtgtacgc tgtggtacca
    24061 tcattgagaa aattcaacta ggtggacgtg gaacccactt ttgtccaaac tgtcaaagga
    24121 gggactgatg ggaaaaatca tcggaatcac tgggggaatt gcctcaggta agtcaactgt
    24181 gacaaatttt ctaagacagc aaggctttca agcagtggat gccgacgcag tcgtccacca
    24241 actacagaaa cctggtggtc gtctgtttga ggctctagta cagcactttg ggcaagaaat
    24301 cattcttgaa aacggagaac tcaatcgccc tctcctagct agtctcatct tttcaaatcc
    24361 tgaagagcaa aaatggtcta atcaaattca aggggagatt atccgtgagg aactggctac
    24421 tttgagagaa cagttggctc agacagaaga gattttcttc atggatattc ccctactttt
    24481 tgagcaggac tacagcgatt ggtttgctga gacttggttg gtctatgtgg accgagatgc
    24541 ccaagtagaa cgcttaatga aaagggacca gttgtccaaa gatgaagctg agtctcgtct
    24601 ggcagcccag tggcctttag aaaaaaagaa agatttggcc agccaggttc ttgataataa
    24661 tggcaatcag aaccagcttc ttaatcaagt gcatatcctt cttgagggag gtaggcaaga
    24721 tgacagagat taactggaag gataatctgc gtattgcctg gtttggtaat tttctgacag
    24781 gagccagtat ttctttggtt gtacctttta tgcccatctt cgtggaaaat ctaggtgtag
    24841 ggagtcagca agtcgctttt tatgcaggct tagcaatttc tgtctctgct atttccgcgg
    24901 cgctcttttc tcctatttgg ggtattcttg ctgacaaata cggccgaaaa cccatgatga
    24961 ttcgggcagg tcttgctatg actatcacta tgggaggctt ggcctttgtc ccaaatatct
    25021 attggttaat ctttcttcgt ttactaaacg gtgtatttgc aggttttgtt cctaatgcaa
    25081 cggcactgat agccagtcag gttccaaagg agaaatcagg ctctgcctta ggtactttgt
    25141 ctacaggcgt agttgcaggt actctaactg gtccctttat tggtggcttt attgcagaat
    25201 tatttggcat tcgtacagtt ttcttactgg ttggtagttt tctattttta gctgctattt
    25261 tgactatttg ctttatcaag gaagattttc aaccagtagc caaggaaaag gctattccaa
    25321 caaaggaatt atttacctcg gttaaatatc cctatctttt gctcaatctc tttttaacca
    25381 gttttgtcat ccaattttca gctcaatcga tcggccctat tttggctctt tatgtacgcg
    25441 acttagggca gacagagaat cttctttttg tctctggttt gattgtgtcc agtatgggct
    25501 tttccagtat gatgagtgca ggagtcatgg gcaagctagg tgacaaggtg ggcaatcatc
    25561 gtctcttggt tgtcgcccag ttttattcag tcatcatcta tctcctctgt gccaatgcct
    25621 ctagccccct tcaactagga ctctatcgtt tcctctttgg cttgggaacc ggtgccttga
    25681 ttcccggggt taatgcccta ctcagcaaaa tgactcccaa agccggcatt tcgagggtct
    25741 ttgcctttaa tcaggtattc ttttatctgg gaggtgttgt tggtcccatg gcaggttctg
    25801 cagtagcagg tcaatttggc taccatgctg tcttttatgc gacaagcctt tgtgttgcct
    25861 ttagttgtct ctttaacctg attcaatttc gaacattatt aaaagtaaag gaaatctagt
    25921 gcgagtaaaa attaatctca aatgctcctc ttgtgacagt atcaattacc taaccagtaa
    25981 aaattcaaaa acccatccag acaagattga ggttttaaag tattgtccca aggaaagaaa
    26041 agtaacccta catcttgaat ctaagtagat tttatggtaa aataataggg attttaagga
    26101 gtttgatatg tataacctat tattaaccat tttattagta ttatctgttg tgattgtgat
    26161 tgcaattttc atgcaaccaa ccaaaaacca atccagcaat gtatttgatg ccagttcagg
    26221 tgatttgttt gaacgcagta aagctcgcgg ttttgaagct gtaatgcagc gtttgacagg
    26281 gattttagtc tttttctggc tagccattgc cttagcattg acggtattat caagtagata
    26341 agaaaataat gggcaggact aggtctttgc ctctttttat ttttaaagga tgtttgagaa
    26401 ggttttacag taaaagaaaa ttaaaaaatc tagaaagaaa atatgaaaga tagaataaaa
    26461 gaatatttac aagacaaggg aaaggtgact gttaatgatt tggctcaggc tttgggaaaa
    26521 gacagttcca aggattttcg tgagttgatt aaaaccttgt ccttaatgga aagaaagcac
    26581 cagattcgct ttgaagaaga tggtagtctg acattagaaa ttaagaaaaa acatgagatt
    26641 accctcaagg ggatttttca tgcccataaa aatggctttg gctttgttag tctggaaggc
    26701 gaggaggacg acctttttgt agggaaaaat gatgtcaact atgctattga tggtgatacc
    26761 gtcgaggtag tgattaagaa agtcgctgac cgcaataagg gaacagcagc agaagccaaa
    26821 attattgata tcctagaaca cagtttgaca acagttgtcg ggcaaatcgt tctggatcag
    26881 gaaaagccta agtatgctgg ctatattcgt tcaaaaaatc ataaaatcag tcaaccgatt
    26941 tatgttaaga aaccagccct aaaattagaa ggaacagaag ttctcaaagt ctttatcgat
    27001 aaatacccaa gcaagaaaca tgatttcttt gtcgctagtg tactcgatgt agtgggacac
    27061 tcaacggatg tcggaattga tgttcttgag gtcttggaat caatggacat tgtatctgag
    27121 tttccagaag ctgttgtcaa ggaagcagaa agtgtgcctg atgctccgtc tcaaaaggat
    27181 atggaaggtc gtctggatct aagagatgaa attaccttta ccattgacgg tgcggatgcc
    27241 aaggacttag acgatgcagt gcatatcaag gctctgaaaa atggcaatct ggagcttggg
    27301 gttcacatcg cagatgtttc ctattatgtg accgaggggt ctgcccttga caaggaagcc
    27361 cttaaccgtg cgacttctgt ttacgtgaca gaccgagtgg tgccaatgct tccagaacga
    27421 ctatcaaatg gcatctgctc tctcaatccc caagttgacc gcctgaccca gtctgctatt
    27481 atggagattg ataaacatgg tcgtgtggtc aactatacca ttacacaaac agttatcaag
    27541 accagttttc gtatgaccta tagcgatgtc aatgatatcc tagctggcga tgaagaaaag
    27601 agaaaagaat atcataaaat tgtaccaagt atcgaactca tggccaagct tcatgaaact
    27661 ttagaaaaca tgcgtgtgaa acgtggagct ctcaattttg ataccaatga agcgaagatt
    27721 ttagtggata aacaaggtaa gcctgttgat atcgttcttc ggcagcgtgg cattgccgag
    27781 cggatgattg agtcttttat gttgatggct aatgaaacag ttgccgaaca tttcagcaag
    27841 ttggatttgc cttttatcta tcgaattcac gaggagccta aggctgaaaa ggttcagaag
    27901 tttattgatt atgcttcgag ttttggcttg cgcatttatg ggactgccag tgagattagt
    27961 caggaggcac ttcaagacat catgcgtgct gttgagggag aaccttatgt agatgtattg
    28021 tccatgatgc ttcttcgctc tatgcagcag gctcgttatt cggagcacaa tcacggccac
    28081 tatggactag ctgctgacta ttatactcac tttaccagtc caattcgtcg ttatccagac
    28141 cttcttgttc accgtatgat tcgggattac ggctgttcta aggaaatagc agagcatttt
    28201 gaacaagtga ttccagagat tgcgacccag tcttccaacc gtgaacgtcg tgccatagaa
    28261 gctgagcgtg aagtcgaagc catgaaaaag gctgagtata tggaagaata cgtgggtgaa
    28321 gagtatgatg cagttgtatc aagtattgtc aaattcggtc tctttgtcga attgccaaac
    28381 acagttgaag gcttgattca catcactaat ctacctgaat tttatcattt caatgagcgt
    28441 gatttgactc ttcgtggaga aaaatcaggt atcactttcc gagtgggtca gcagatccgt
    28501 atccgtgttg aaagagcgga taaaatgact ggagagattg atttttcatt cgtacctagt
    28561 gagtttgatg tgattgaaaa aggcttgaaa cagtctagtc gtagtggcag agggcgtggt
    28621 tcaaatcgtc gttcggataa gaaggaagac aagagaaaat caggacgctc aaatgataag
    28681 cgtaagcatt cacaaaaaga caagaagaaa aaaggaaaga aaccttttta caaggaagta
    28741 gctaagaaag gagccaagca tggcaaaggg cgagggaaag gtcgtcgcac aaaataaaaa
    28801 ggcacgccac gactatacaa tcgtagatac gctagaggca gggatggtcc tgactggaac
    28861 tgaaatcaag agtgtacgag ctgctcgaat taatctcaag gatggctttg ctcaagtgaa
    28921 aaatggagaa gtttggctga gcaatgttca tatcgcgcct tacgaagagg gcaatatctg
    28981 gaaccaggaa ccagaacgtc gtcgtaaact cttgctccat aaaaagcaaa ttcaaaaatt
    29041 ggaacaagag accaaaggga caggaatgac cttagttccc cttaaggtct atataaaaga
    29101 tggctacgct aagcttcttt taggacttgc caaagggaag catgactatg acaaacggga
    29161 gtctatcaaa cgtcgtgagc aaaatcgaga tatcgcgcgt gtgatgaaag ctgttaatca
    29221 gcgataaaaa gaggaattga aaatggaaaa attagttgcc tataaacgca tgcctttgtg
    29281 gaataaacaa acaatgcctg aagctgttca gcaaaagcac aatacaaaag ttgggacttg
    29341 ggggaaaatt actgtcttga agggagctct caagtttatt gaattgacag aagaagggga
    29401 agttctagct gaacacctct ttgaagcagg ggcagacaat ccaatggccc aacctcaagc
    29461 ctggcaccga gtggaagctg ccacagatga tgtggaatgg tacttggaat tttattgtaa
    29521 acctgaggat tattttgcta aaaaatacaa taccaatcct gttcattcag aggtcctaga
    29581 ggccatgcag acagtgaaac aagggaaagc tttggatttg ggttgtggtc aggggcgtaa
    29641 ttctcttttt ctagcccagc aagattttga tgtgacggct gtagatcaaa atggactagc
    29701 tcttgaaatc ttgcaaagca ttgtggagca ggaagatttg gacatgcctg ttggccttta
    29761 cgatatcaat tcagctagca ttgaacaaga atatgatttt atcgtttcaa cagttgttct
    29821 catgtttcta caagcggacc gcattccagc tattattcaa aatatgcagg agaaaaccag
    29881 tgttggcggt tacaacctta tcgtttgtgc catggacacg gaggattatc cttgctcggt
    29941 taacttccca ttcaccttta aagaaggaga actggcagac tattacaagg attgggaatt
    30001 ggttaagtac aatgaaaatc caggccattt gcaccgtcgc gatgagaatg gcaatcgtat
    30061 tcaactacgc tttgcgacct tactagctaa gaaaatcaag taaacacaca tgaagattag
    30121 gaattttcct gatctttttt cttttttacg aatgatatag aaaaggaggg aattcatgtt
    30181 tgttgcgaga gatgctaggg gagaattggt aaatgtgtta gaggataaac ttgagaagca
    30241 agcatacacc tgcccagctt gtggaggcca gctccatttg cgtcaaggac caagtgtacg
    30301 gacgcatttt gcccataaat ccttaaaaga ctgtgatttt ttctttgaaa atgaaagtcc
    30361 agaacacctg gccaataagg aatccctcta tcactggttg aaaaaagaga caaaggttca
    30421 attagagtac ccgctttcag aacttaaaca gattgcggat gtatttgtaa atggcaatct
    30481 agctctagaa gttcagtgta gtcccttgcc tcagaaagtc cttaaagagc gaagtgaggg
    30541 ctatcgtagt cagggttacc aagtactgtg gttgctgggt caaaaactgt ggctcaagga
    30601 gcgtttgact cgtctacagc aaggttttct ttatttcagt caaaacatgg gcttttatgt
    30661 ttgggaatta gacaaggaaa aacaagtttt aagactcaaa tacctgattt accaggatct
    30721 ccgcggtaaa ctccattatc aaatcaagga attttcctat ggtcaaggta gtttattgga
    30781 aatattgcgt cttccctata agagacaaaa aatatctcat tttacagttt ctgaggacaa
    30841 ggacatctgt cgctatatcc ggcaacaact ttattatcaa aatctctttt ggatgaaaga
    30901 acaagcagaa gcctatcaaa agggagaaaa tatcctgact tatggactga aagaatggta
    30961 tccacaaatt cgaccaatag tgggcaaatt tttccagatt gaacaagact tgactagcta
    31021 ttatcagcac ttttatacct attaccaaaa aaatcctcaa aatgattggc aaaagcttta
    31081 tccaccagcc ttttatcagc aatatttctt gaaaaatatg gtagaataga aaggatggag
    31141 gaatctaatg gtattacaaa gaaatgaaat aaatgaaaaa gatacatggg atctatcaac
    31201 gatctaccca actgaccagg cttgggaaga agccttaaaa gatttaacag aacaattgga
    31261 gacagtagcc cagtatgaag gccatctctt ggatagtgcg gataacctac tagaaatcac
    31321 tgaattttct cttgaaatgg aacgccagat agagaagctt tacgtttatg ctcatatgaa
    31381 gaatgaccag gatacacgtg aagctaagta tcaagagtac tatgccaagg ccatgacact
    31441 ctacagccag ttagaccaag ccttttcatt ctatgagcct gaatttatgg agattagcga
    31501 aaagcagtat gctgactttt tagaagctca accaaagctg caggtttatc aacactattt
    31561 tgacaagctc ttgcaaggca aggatcacgt tctttcacaa cgtgaagaag aattattggc
    31621 tggagctgga gaaatctttg gttcagcaag tgaaaccttc gctatcttgg acaatgcgga
    31681 tattgtgttc ccttatgtcc tagacgatga tggtaaagaa gttcagctat ctcatgggac
    31741 ttacacacgt ttgatggagt ctaaaaaacg tgaggttcgc cgtggtgcct atcaagctct
    31801 ttatgctact tacgaacaat tccaacacac ctatgccaaa accttgcaaa ccaatgttaa
    31861 ggtgcaaaat taccgtgcta aagttcgtaa ctacaagagt gctcgtcatg cagccctcgc
    31921 agcgaatttt gttccagaaa gtgtttatga caatttggta gcagcagttc gcaagcattt
    31981 gccactctta catcgctatc ttgagcttcg ttcaaaaatc ttggggattt cagatctcaa
    32041 gatgtacgat gtctacacac cgctttcatc tgttgaatac agttttacct accaagaagc
    32101 cttgaaaaaa gcagaagatg ctttggcagt cttgggtgag gattacttga gccgtgttaa
    32161 acgtgccttc agcgagcgtt ggattgatgt ttacgaaaat caaggcaagc gttcaggtgc
    32221 ctactctggt ggttcttacg ataccaatgc ctttatgctt ctcaactggc aggacaatct
    32281 ggacaatctc tttactcttg ttcatgaaac aggtcacagt atgcattcaa gctatactcg
    32341 tgaaactcag ccttatgttt acggggatta ctctatcttt ttggctgaga ttgcctcaac
    32401 taccaatgaa aatatcttga cggagaaatt attggaagaa gtggaagacg acgcaacacg
    32461 ctttgctatt ctcaataact tcctagatgg tttccgtgga acagttttcc gccaaactca
    32521 atttgctgag tttgaacacg ccattcacca agcagatcaa aatggggagg tcttgacaag
    32581 cgatttccta aataaactct acgcagactt gaaccaagag tattatggtt tgagtaagga
    32641 agacaatcct gaaatccaat acgagtgggc tcgcattcca cacttctact ataactacta
    32701 tgtatatcaa tattcaactg gctttgcggc cgcctcagcc ttggctgaaa aaattgtcca
    32761 tggtagtcaa gaagaccgtg accgctatat cgactacctc aaggcaggta agtcggacta
    32821 tccacttaat gtcatgagaa aagctggtgt tgatatggag aaggaagact acctcaacga
    32881 tgcctttgca gtctttgaac gccgtttaaa tgagtttgaa gcccttgttg aaaaattagg
    32941 attggcataa aatggttgaa tcgtatagta agaatgctaa ccataacatg cgtcgtcctg
    33001 tcgtcaaaga agaaattgta gacttgatgc gtcagcgtca aaagcaggtc acaggtttct
    33061 tgaaagaatt ggaagacttt gcccgcaagg aaaatattcc tattattccc catgaaacgg
    33121 ttgcttattt ccgttttctt atggaaacca tgcagcctaa aaatattctg gaaattggga
    33181 cggctatcgg tttttcagct ctcttgatgg ctgaacatgc gccaaatgct aagattacaa
    33241 ctattgatcg taatccagaa atgattggtt ttgccaagga aaattttgcc cagtttgaca
    33301 gtcgcaagca aatcactctc ctagagggag atgcggtgga tgtcttatct acactgacag
    33361 agtcttatga tttcgtattt atggattctg ccaagtctaa atacatcgtc tttctgccag
    33421 aaatcctcaa acatttggaa gttggtggtg tggttgtctt ggatgatatt tttcaaggtg
    33481 gtgatgttgc caaggatatt atggaagtcc gtcgtggtca gcgaaccatt tatcgaggcc
    33541 ttcaaaaatt atttgatgca accttagaca atccagaact caccgcaaca ttagtgcctt
    33601 taggagatgg tattctcatg cttcgtaaaa atgtagcaga tgttcaactg tctgaaagcg
    33661 aatgattttc agaaaaattt aagaaaaaat agtaaaatag atagagtaac acttatctca
    33721 aaggagtaga catgaagaaa aaattattgg caggtgccat cacactatta tcagtagcaa
    33781 ctttagcagc ttgttcgaaa gggtcagaag gagcagacct tatcagcatg aaaggggatg
    33841 tcatcacaga acatcaattt tatgagcaag tgaaaaacaa cccttcagcc caacaagtct
    33901 tgttaaatat gaccatccaa aaagtttttg aaaaacaata tggctcagag cttgatgata
    33961 aagaggttga tgatactatt gccgaagaaa aaaaacaata tggcgaaaac taccaacgtg
    34021 tcttgtcaca agcaggtatg actcttgaaa cacgtaaagc tcaaattcgt acaagtaaat
    34081 tagttgagtt ggcagttaag aaggtagcag aagctgaatt gacagatgaa gcctataaga
    34141 aagcctttga tgagtacact ccagatgtaa cggctcaaat catccgtctt aataatgaag
    34201 ataaggccaa agaagttctc gaaaaagcca aggcagaagg tgctgatttt gctcaattag
    34261 ccaaagataa ttcaactgat gaaaaaacaa aagaaaatgg tggagaaatt acctttgatt
    34321 ctgcttcaac agaagtacct gagcaagtca aaaaagccgc tttcgcttta gatgtggatg
    34381 gtgtttctga tgtgattaca gcaactggca cacaagccta cagtagccaa tattacattg
    34441 taaaactcac taagaaaaca gaaaaatcat ctaatattga tgactacaaa gaaaaattaa
    34501 aaactgttat cttgactcaa aaacaaaatg attcaacatt tgttcaaagc attatcggaa
    34561 aagaattgca agcagccaat atcaaggtta aggaccaagc cttccaaaat atctttaccc
    34621 aatatatcgg tggtggagat tcaagctcaa gcagtagtac atcaaacgaa tagtccaaat
    34681 caatgagtca gggaaaaaac tctacttcag gaaaaaatga agcaaacatt cccacaataa
    34741 aacgcgtagt acaaggtttg tactgacccc caaaagttag acaaataatt tatccgaagg
    34801 atttagttct gtattgcaca gggctaagtc cttttagttt taccttaatt ctcttgttgt
    34861 tgtaataatc aatatagtct ataatggctt gttccaattg attaagtgat ttaaatgttt
    34921 tctcatagcc ataaaacatt tcggatttta aaataccaaa gaaagattcc atcctaccgt
    34981 tgtcttggct gtttcccttg cgtgacatag atgcttgaat tcccttactc tctaggaacc
    35041 gatgataaga atcgtgttgg tattgccagt cttggtcact atggagaatc gtattctcgt
    35101 agtgcttctc tttgaatgcc tgttccaaca ttaacgatca atcaatttaa tcatgtacct
    35161 aatattagaa ttgtttatct caaatttatt tgaaa
//
LOCUS       DAVKFH010000016        35009 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKFH010000016 DAVKFH010000000
VERSION     DAVKFH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.016"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(80..505)
                     /locus_tag="VW006_000853"
                     /pseudo
     CDS             complement(80..505)
                     /locus_tag="VW006_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(581..793)
                     /locus_tag="VW006_000854"
     CDS             complement(581..793)
                     /locus_tag="VW006_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9379733.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(802..1107)
                     /locus_tag="VW006_000855"
     CDS             complement(802..1107)
                     /locus_tag="VW006_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEU9379734.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1094..1288)
                     /locus_tag="VW006_000856"
     CDS             complement(1094..1288)
                     /locus_tag="VW006_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9379735.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(1458..1808)
                     /gene="tnpB"
                     /locus_tag="VW006_000857"
     CDS             complement(1458..1808)
                     /gene="tnpB"
                     /locus_tag="VW006_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9379736.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(1789..1974)
                     /locus_tag="VW006_000858"
                     /pseudo
     CDS             complement(1789..1974)
                     /locus_tag="VW006_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2278..3840)
                     /gene="guaA"
                     /locus_tag="VW006_000859"
     CDS             complement(2278..3840)
                     /gene="guaA"
                     /locus_tag="VW006_000859"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9379737.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CLYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3982..4680
                     /locus_tag="VW006_000860"
     CDS             3982..4680
                     /locus_tag="VW006_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9379738.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4723..5619)
                     /locus_tag="VW006_000861"
     CDS             complement(4723..5619)
                     /locus_tag="VW006_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9379739.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(5628..6563)
                     /locus_tag="VW006_000862"
     CDS             complement(5628..6563)
                     /locus_tag="VW006_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9379740.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6560..7285)
                     /locus_tag="VW006_000863"
     CDS             complement(6560..7285)
                     /locus_tag="VW006_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9379741.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(7369..8004)
                     /locus_tag="VW006_000864"
     CDS             complement(7369..8004)
                     /locus_tag="VW006_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9379742.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8006..8833)
                     /locus_tag="VW006_000865"
     CDS             complement(8006..8833)
                     /locus_tag="VW006_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9379743.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8855..9190)
                     /locus_tag="VW006_000866"
     CDS             complement(8855..9190)
                     /locus_tag="VW006_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEU9379744.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(9191..9907)
                     /locus_tag="VW006_000867"
     CDS             complement(9191..9907)
                     /locus_tag="VW006_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU9379745.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            10420..11031
                     /locus_tag="VW006_000868"
     CDS             10420..11031
                     /locus_tag="VW006_000868"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9379746.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11076..11804
                     /gene="rlmB"
                     /locus_tag="VW006_000869"
     CDS             11076..11804
                     /gene="rlmB"
                     /locus_tag="VW006_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9379747.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(11843..12754)
                     /gene="trxB"
                     /locus_tag="VW006_000870"
     CDS             complement(11843..12754)
                     /gene="trxB"
                     /locus_tag="VW006_000870"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9379748.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12820..13044)
                     /locus_tag="VW006_000871"
     CDS             complement(12820..13044)
                     /locus_tag="VW006_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9379749.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13122..13865)
                     /locus_tag="VW006_000872"
     CDS             complement(13122..13865)
                     /locus_tag="VW006_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9379750.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13865..14665)
                     /locus_tag="VW006_000873"
     CDS             complement(13865..14665)
                     /locus_tag="VW006_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9379751.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14823..15179)
                     /locus_tag="VW006_000874"
     CDS             complement(14823..15179)
                     /locus_tag="VW006_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9379752.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15192..15704)
                     /locus_tag="VW006_000875"
     CDS             complement(15192..15704)
                     /locus_tag="VW006_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9379753.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15704..16198)
                     /locus_tag="VW006_000876"
     CDS             complement(15704..16198)
                     /locus_tag="VW006_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379754.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16333..16779
                     /locus_tag="VW006_000877"
     CDS             16333..16779
                     /locus_tag="VW006_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9379755.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16776..17423
                     /locus_tag="VW006_000878"
     CDS             16776..17423
                     /locus_tag="VW006_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9379756.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17444..18025)
                     /gene="pdxT"
                     /locus_tag="VW006_000879"
     CDS             complement(17444..18025)
                     /gene="pdxT"
                     /locus_tag="VW006_000879"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9379757.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18026..18901)
                     /gene="pdxS"
                     /locus_tag="VW006_000880"
     CDS             complement(18026..18901)
                     /gene="pdxS"
                     /locus_tag="VW006_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9379758.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19053..20432)
                     /locus_tag="VW006_000881"
     CDS             complement(19053..20432)
                     /locus_tag="VW006_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9379759.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20726..21649
                     /locus_tag="VW006_000882"
     CDS             20726..21649
                     /locus_tag="VW006_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9379760.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21709..22314
                     /locus_tag="VW006_000883"
     CDS             21709..22314
                     /locus_tag="VW006_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9379761.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22333..23577
                     /locus_tag="VW006_000884"
     CDS             22333..23577
                     /locus_tag="VW006_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9379762.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSS
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23800..24057)
                     /locus_tag="VW006_000885"
     CDS             complement(23800..24057)
                     /locus_tag="VW006_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9379763.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24099..26135)
                     /locus_tag="VW006_000886"
     CDS             complement(24099..26135)
                     /locus_tag="VW006_000886"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9379764.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26395..27312)
                     /gene="glyQ"
                     /locus_tag="VW006_000887"
     CDS             complement(26395..27312)
                     /gene="glyQ"
                     /locus_tag="VW006_000887"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9379765.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(27331..28092)
                     /locus_tag="VW006_000888"
     CDS             complement(27331..28092)
                     /locus_tag="VW006_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9379766.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(28365..29207)
                     /locus_tag="VW006_000889"
     CDS             complement(28365..29207)
                     /locus_tag="VW006_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9379767.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLESLKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(29322..30713)
                     /locus_tag="VW006_000890"
     CDS             complement(29322..30713)
                     /locus_tag="VW006_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9379768.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(30948..31325)
                     /locus_tag="VW006_000891"
     CDS             complement(30948..31325)
                     /locus_tag="VW006_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9379769.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            31540..32517
                     /locus_tag="VW006_000892"
     CDS             31540..32517
                     /locus_tag="VW006_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9379770.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            32514..33596
                     /locus_tag="VW006_000893"
     CDS             32514..33596
                     /locus_tag="VW006_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9379771.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTINLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(33660..34690)
                     /locus_tag="VW006_000894"
                     /pseudo
     CDS             complement(33660..34690)
                     /locus_tag="VW006_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10388 a         7787 c         6278 g        10556 t
ORIGIN      
        1 ttcaaaactt tgagaaaata accaccctcg cttttttaaa ctgagcttag gctaaaagag
       61 tccactggac tcttttactc cgtcccataa ccaatgattt aatggcgcat tcagctagat
      121 tattggaaag gaccagatgt ccgtctttca aaatagtctt aaaggtttct ttatacttga
      181 ggctatactt gaggctgtat tcaattgccc ttcctagttt tgaacccgat aaaactgact
      241 gacgccggca ccaagcaaag aaatcttcca ttaggggttg gagatgttct tgacgtttct
      301 gtagtcgttc atcagctggc aaagcctccc agtttctttc caaggaaaat aactgattac
      361 aataagctaa acctttagct cctaaggatg atttattcgc ttgcttgggg gtcgcttcaa
      421 aaaacttcct tctcacatgt acccaacatc caacaagttc agcttcttcc agttgacaat
      481 aagcttctag cggttcgcag tttaccaagc ttagcgtcgc cttgctccta aaccttggac
      541 tgctatcgca taggcagaac tagaggacta aagtcctaag ttactgccgc aacatatcac
      601 aatgcacata gccagaataa tctcctagga attcttgcac taccgaacca ctccgacgct
      661 gatcatggtg gtacagcgtg attgcttgat tctcagcttt cccagacaaa aaggtccaat
      721 agtaggtcag atggctatca ttctctagaa cccgatagga ggtttcatct gcatgaagta
      781 agggctgaat caatagtctc gttataaagg ggctttagga ataggagctt ttacaatttt
      841 atcacttgga ttattcttac tgcatgtttg gcatttgtaa gcgtgttgga tatgatccac
      901 tctttttaat tgcgcaggaa taaagaccaa ttcttgtcgt tgaaggctcg ctccaatctc
      961 ttttaaatcg tcctgacaat cagggcaaat gctctcttct acttgatgat gaacttcttc
     1021 tgaatcaaat tgggcaagaa tagcttgacg tttcccttta gatttcttac gtttgtaggt
     1081 aatttcctct gtttcaactg ggaacatctc catctttctc agatggagac tcttcctcaa
     1141 aaaggttgag ttgtccaaaa gggcaaacac ttttctcaga ggattttcca gagagctttt
     1201 gcgttagata agccacttgt tcacacagaa gggaaagttc attagtgaga ctatcaatta
     1261 tagcactctg ttgttgaatg attttcaatc ttctcccata gactttcctc cttatttcct
     1321 aactttatta tatagtgtat tgaatctata acagtacacc ttgactgcta aaatatttct
     1381 ataaattaat ttgactttcc tgatagagat gttcatatct tatttcaatt cactatacta
     1441 aaaagaaagt cctcatttca atagaaatca cgactttctg ataaatttat tttgggagtg
     1501 atagaaaagc ccttcataag ccaatctact tgttcaggtg tgagagcttt gacatccttt
     1561 tctgtactgg gccaagtcag tctgccgttc tcaaagcgtt tatatagtag ccaaaatcct
     1621 tgaccatccc agtaaagggc tttaaagcgg tctttacgtc caccacaaaa gagaaaaact
     1681 tgaccggaga aaggatccaa ttcaaagtgg gttttaacca gataagccag tgaatcgatt
     1741 ccttgtctca tatcagtctt cccacacacg agatagacct gccctaggct agatagatga
     1801 attgtcatag agcaacacct tatccaaaag ctgttctacc atttcgagat tgagagattg
     1861 aaaaaaactt acttcaagct tgccaatacg aatttaaagc agaatatcat ttctcttttt
     1921 agaatcaaaa ggacgagatt gtggaatctc tacaggaaca ataggttgtg gcattaaaat
     1981 atcctccaat agttttactt tactaatagt atacaggagg aactccatga atgatagata
     2041 ccttgttatg acgcgcttac tatattagaa agttggctct ctaattaaat taattctcat
     2101 tatatatatc aaaagaccgc agtttgaaaa ctacggtcta attttttgtc caaatgtaaa
     2161 tcacttggaa ctattattca atttattctt tttagtaaat gttcccgttt taatcccagt
     2221 tacagttttt aatcctctgt aaccgttgat tttacaaagc ttttaatttt tttgcgatta
     2281 ctcccactca actgttgcag gtggtttact tgtaatatcg tagacgatac ggttaacatg
     2341 atccacttca tttacgatac gtactgagat tttttgaagt acttcccatg gaattttggc
     2401 aaaatcagca gtcataccat cgatagaagt gatagcacgg attgcaatcg tgtagtcata
     2461 cgtacgaccg tcacccataa caccgactga acgaacgcct gtgttaacag tgaagtattg
     2521 ccaaatatcg cggtcaagtc cagctttagc gatttcttca cgaagaatag cgtctgattc
     2581 acgaacggtt tcaagtttct cttcagtgat ttcacccatg acacggatag caagtcctgg
     2641 tcctgggaat ggttggcgcc atacgatatg gtctggcata ccaagctctg taccaagagc
     2701 acgaacttca tccttgtaaa gagtattgag tggttcaatc aattcaaact gcatatcttc
     2761 tggaagacca cctacgttgt ggtgtgactt gatagtttga gctgtatccg taccagactc
     2821 gataacatct gtatataaag taccttgagc aaggaatttc acatctttga gcttgcttgc
     2881 ttcgtcatcg aatacataga caaactcgtt accgatgatt ttacgttttt gttcaggatc
     2941 agaaacgcca gcaagtttgt caaggaaacg tttagcagcg tctgctttga cgatattcaa
     3001 accaaactta ccaccgagca tgtccataac ttgatcagct tcgcctttac gaagaagacc
     3061 gtggtctacg aagatacaga tcaattgatc gccaatcgct ttttggagaa gaaccccaac
     3121 gacagatgag tcaacaccac ctgatagacc aagaaggaca cgtttatcac cgacggtttc
     3181 acgaattttt ttgatctgca tgtcaatgaa attatccatt gaccagtcac ctttagcctt
     3241 acaaatgtta agggcaaagt tacgaaggat atcatttccg tatacagaat gacgaacttc
     3301 tgggtggaat tggataccgt aaatgtgttt atctgggttt tcgatggctg cgtataggca
     3361 gtcagctgat gtacctgtac gaacaaagtc agcaggaatc tcagtaaccg catcaccatg
     3421 gctcatcaaa acagtctgtt catcaggtgt tgattcaaaa agcgctgatg gtgtgtgagt
     3481 tagggttgat tgaccgtatt cacgatttcc agcatcacct gcaggaacaa cttttcctcc
     3541 aagtttatgg gtcaataact gcataccata acagattccc aaaattggaa ttccgagttc
     3601 gaagatttct gggtcaatat caaatgaacc atcttcatat acagaatttg gaccacctga
     3661 tagaataatt cctacaggat tgacttcacg aacttcagca gctgaaattt tatggctttt
     3721 tagttctgaa aaaacaccaa tctcacggat acggcgtgaa atcagctggt tgtactggct
     3781 accatagtcc aatacgatga ttttttctac atcttgcaaa tcagttgaaa tgttgctcat
     3841 ctttttcctt tctcaaaaga aatatctttt ctcaatattc tatcacaaat aagggcgaat
     3901 taccaactaa acaaggcgaa gtttgacttt ttctagttta ttggggtaca atagagaaaa
     3961 gatagaaatg aagtgaaaaa tatgctacct gcttatatga aaatccatga tcagataaaa
     4021 aaggatattg acgagcaccg ttgggctatt ggtgagaggc ttcccagtga aagagattta
     4081 gctgagcagt ttgcggtcag tcgcatgacc ctccgccaag ccgtatctct attagtcgaa
     4141 gaaggcgtct tagagcgccg tgtaggaagc ggcacctttg tttccagtac tcgagtacaa
     4201 gaaaagatgc gagggacaac cagttttact gaaattgtca aatcccaagg taaagttccc
     4261 tctagccagc tcatttccta caaaaaaacc attcccaatg agcaagaagt tgccaagcta
     4321 ggaatttttc caacggacaa tattatccga atggagcggg tccgctatgc cgaccaagtt
     4381 cccctagttt atgaagttgc ttctattcct gaaaaattca ttaaggactt taaaaaagaa
     4441 gaaatcacca gtcatttctt ccaaaccttg caaaaacatg gctatcgtat cggcaaatct
     4501 caacagacca tctatgctcg ccttgctaaa gaaaagattg cccactattt ggaagttgaa
     4561 aaaggacatg ctattcttgg attgacccag gtttcctacc tagaagatgg tactgctttt
     4621 gaatacgtaa aaagtcaata tgtaggcgaa cgctttgaat tttatcttga aaataattag
     4681 attaccacgc aaaaactctc tgtcacggac aaagagtctt ttttattttt ctaagaataa
     4741 atctttcaaa gaaatcaaag ttacagcaaa caatatcatt gccataccaa taaaatcaat
     4801 gggataaaat tgttcattca ttattaagaa ggcaaagaaa accgccgata ttggctcaat
     4861 tgaagccaac aagcttgact tgaccggtcc tatcagactg gctcctttaa ggaaagctgt
     4921 ataggcaaag acagtcccga taaggataat gcctgcaaac gcaaggagaa aatcaagact
     4981 agtcgggata tcggcctgta gaacccctgt aaaaggaagg gcgaccaaac ctgctatgac
     5041 catccccaca ccaatgacca agctgctccc ccacttttta atcaaggcta tgggtaaaat
     5101 gatatacaaa gcataagtca aggcagaaaa gagaccccag aacaggccag caggtgtcat
     5161 ggataactgg tccaactgcc catgtgttgc gatcaggaag gttcctccga tggcgaatat
     5221 gatggaaact atctctccca gtgtcggtgc caccctatcc ttgatacagc tataaattaa
     5281 aattccgaca ggacaaacat actgaagcac cgtcgctgtt cccgcattgg tctcctgaat
     5341 agcagacaga taggcgaatt ggttgagaaa aagaccaatc agagcaaaaa taagaagaga
     5401 cagcaaactc tttctatcct ttaaaaagac cagtatttta tcctttgcag tagcataagc
     5461 caagagcatg agaattccac cagcgattaa aagacgcaag ttggtcaaga ccagagccga
     5521 aattccgtgt gccattaggt attggccact cgttcctgac aacccccaag caataccagc
     5581 cacaactgtt agtaaagtcc cttttaaact atttgacatg tctctcctta ctcttctttg
     5641 cgaatcaagt tcatcacttg attgcggtct aatatccatt gagctctctc gtccttttca
     5701 taggtacgat tcgataggaa aatagtggct tcttgcttct gacgattcca catgataaag
     5761 gtacctgtat agcccgtatg gtctagccaa tctccttcca aattccatgc taaagaacgt
     5821 tccttgtcat ccaaaggaga aaaattttga tttaagtctc ttgcaaaatc atctgctaaa
     5881 tagtgttcta aaaagatttg taaatccttt atagtcgaaa ataaaccagc actcccagca
     5941 tgtctaccca ggagacgagc cttgggatca tgcactatgc ctgcctctac acctctaact
     6001 gttggaacag caagctcaac tggcccaaac ttggtttccg tcattcccca aggtttccag
     6061 acttgatcct ttaaaatcac atccaaatct tgattaaaaa ttctttccaa aataaagccc
     6121 aacagcaaaa aatggacatc cgaataaaga aaggctggct gacttcgtct gttgagatga
     6181 aacatcgctt cctttaattc aggggctgtt aaaagatcac gattaggaat aaaaggatca
     6241 aggtctgttg catgagtcaa gagctggcga atagtgatgt ctggataatc actctcaggt
     6301 aaaaaatcta ttaccagtct atcaatatct aattgaccta tttcccacaa gaaggtacaa
     6361 actgtgccaa ccccaacaac cttgctgaca ctagctaggt cataaactag tcctgcctca
     6421 gtctgcaagc catgctctgg gtcactctgg cctaaataga actctgtcca ttgattgtcc
     6481 ttaaaatacg caaaagaggc tccgggataa attcctgcct cgatttgttc ttctattttt
     6541 ttaataatct tggtccactt catacttctt caaaccacaa ttcaacatta tttctatctt
     6601 tacaaaggaa gaatttttca gacttaggaa taaaatattc agtagactca aacttctggc
     6661 gaagacttgc tatgtctaat tcattgacca agaacttgag catagataag tcccaggtaa
     6721 ccgtattgtc cacagtcaaa tcctgcccct gagctggaat aaaatcaagg gatgccccaa
     6781 tttcagatga ttccaagaaa ctttcgatat cagttgggag atgtaattcc atagaaatct
     6841 caaatttact taaagaaatt gatgccaaat ctgtttgaaa ttcaggcttt tctcctactt
     6901 ctactagact tgctatgtca tcttccgcat gaatcaaaat caaatcatct tctggtgaga
     6961 aaatttcaaa agcgtagcca ttttgacctt tatataatcg atgaatcgaa tctgttttag
     7021 ataagattcc ttcaatttct aagggatttt ccaccttgac aatcaatcta gctagttttt
     7081 ttcttccctc taccttacga gtacgcatac tgggagcttc ttctaaaacc agcttttcaa
     7141 gacccgtttg gtcacctagt gacagaaagg ccgattcttc taacaaggcc ttcattccaa
     7201 gggtttcaat ataaaatgtt tcatttaatt ttctattatt agcttttaaa gtaggaataa
     7261 tccgtacaat ctgatttaca ttcataaatt cctccgtcac tttttatttt aaaggatttt
     7321 agaatttttt acaagatatt atgctcaaat ttaacattat ttagctattt agtaaagata
     7381 gtctttcaag gattttgaca aagcctgata accagcaata ctgaggtgca gtccatcagt
     7441 tgtatattct tttttgagtt ggcctgcttg gtctgtcaaa caatcaaata ctggcacaaa
     7501 ttccacctgc atataggcag atgcaagctc ttgataggct tgattccagt tctgaatttt
     7561 ttcattcgag cggatataga ctgcctgctg gtactcctct ctctcattga caggcaaaat
     7621 ggaaagcaat ttaatctctg tcaatggata atcgcgagca acggattgaa tgatagcttc
     7681 gagattattg agagcctcat tcacaggaac atcctttccg atatcatttg tcccaatcag
     7741 aagaaaaatt ttatctactg ctccaccata tagatgagca tcaaggttct ctagtaacag
     7801 tcctgtctga tagccacgaa ttcctcgatt gacaatcgtc tttgaagtcc caaatagctc
     7861 ctgtagagga taatactcga caatggaatc cccaataaaa agaatgtttg gttctacaac
     7921 agaaatgtga tttaggtgac gatacttagt ttgaattttt tcttgttcct ttaggagcca
     7981 attttctaat aactgtactg ccacttcatt ctcctttttc taaccagttt tccaagactt
     8041 gataaactcc tccttggctg ttggctggtg ctagagcagt cgccacagct ttggctttct
     8101 catcagcatt ttccatcgca taggcaattc cagccatttc aagcatttca acatcatttt
     8161 cactatcacc aaaagccatg atttcttggg atttcaagtc ccagcgcttg agtaattcct
     8221 ccaagcccca tgctttatga atcccagctt ggaggatatc aatgcaacca tagccactgg
     8281 atacagctcg gacacggcca tcaaagagag cattgatttc ttccaaaacc gaactcaaac
     8341 gttcctcacc aacaaccatg ctcatcttga gcacaccacc aaagaggtca gatgttaatt
     8401 catccacaaa ttgcatccgt tggtagaatt tttcaatcat ttctggagtc ataaaacttt
     8461 caagatctgt aaaaatcgta ccttccttga caaaatcacc cttcatcccc gttacaacaa
     8521 actggtcctg acacgctcga cccttgaaat gagtcaaagc cttgttgaca atggcgtcat
     8581 cccatgtctg agcctgaatc aattcattgt tttcaaaaat acgagcgcca ttagcaacaa
     8641 ccagaaccac tcgatccacc aagggactca gtagttgtct catgcggtga atttcattgc
     8701 ccgtcgcaat gacaaaacga atgccccttt gatccaactg atctaaaatc ttttccaatc
     8761 gtgggagatc aagctgacct ctagcatcca gcaaggtccc atccatatct gtcgcaatta
     8821 ccttaatcat catttacttt cctctggatt caagctaata ccacttcaat cccacatgtt
     8881 cattcatttc tttataggct gtatcaattc gttccttagt cgcttcatct aactggtcac
     8941 gatgactgcc atcctctata aaatcttcca aaatataagt ttggtaatga tacaagggat
     9001 atccttctgg tgaaaaggtt ccctttggtg ttcgattgac ccaagtagga tgagctttag
     9061 ctgttccgat agttgttttt ccatccttct tcttgatggt gacatccatg agaacaccac
     9121 gttcagtcca cttagcattc tcttcatctc ccatagattc aattcgttga ttggaaatga
     9181 agttccccat ttaatagaca atgagctttt tatctccatc tttttccacc gtttcagatg
     9241 gttcaacaac gtgaggatgc cctccaaaga taatatccgc tccccaatca atcatcttgt
     9301 gataaagagc tttttgttct tcagttggtt ccaatcgata ctcaacaccc atctgaggca
     9361 tgataatggt gatatctgct tccttctctg cccgttcaac ttcagccttt atcttatctt
     9421 cgtttaaatc tgaaagataa cgattatagt cttcctgaga aatatactgc tcaattccat
     9481 tgaaaccata ggaataggct aacaatgcaa ccttgatagc attcacttcc ttaatgacca
     9541 gcggagcctg agcacgtggt tcgtgcgtat aaactccgat tggagtgatt ccagctttct
     9601 caataatatc ggccattgaa ataactccct caatttgcga atccaaaata tgattatgag
     9661 ctaaatccag cacatgataa cctgcctcct taatagcatc cataacttca acaggagcat
     9721 taaagagaag ataacccgct aaataatgat ccttattaat ggttccttca aaatcaccaa
     9781 tagctaaatc tgcttgcttg agccaaggag tcacatactc aaaattttca tgaaagtcat
     9841 aggtaccgtc ttctttttta gctgaaaaga aaagtccatc gtggtagagc aaatccccgt
     9901 gggccataat tctggcagtt ttttcctctt cctactcctg agacttttgc ttagtcccct
     9961 cttgagaaat agtatctcgt ttttgactag tcaagggatt ccttggaagc tttcaaacaa
    10021 caagaccaag cccattgata aaccaactgc tagcaagagt atcgccacaa atcccttatt
    10081 gctccacttg cgataactcc taaaaagttt accaagccct tcataaaacg aaaagctgaa
    10141 ccaccctgat ttcgattttg tcttctttgt atcttcgttc tccctacttt cttatgcaag
    10201 ccttttcttt ttattatatc acagataagt atttctttca caattgaatt gaacttccta
    10261 tctattttct ataaatccta aatgccataa tgctttcaat tcttgtcatt ttgtgatatc
    10321 atgtagagga aatgaactaa tccacagtgg cttattccaa gtataccact tgggctttgg
    10381 cagtagctaa ctgcactaaa cataatataa ggagagaaaa tgtctgcaat agaacgtatt
    10441 acaaaagctg ctcacttaat tgatatgaac gatattatcc gtgaagggaa tcctactcta
    10501 cgcacggttg ctgaggaagt cactttcccc ctatctgacc aggaaatcat cctaggcgaa
    10561 aagatgatgc aattccttaa acattcccaa gatcctgtca tggctgaaaa aatgggactc
    10621 cgcggtggtg ttggactggc tgctccccag ttagatatct caaaacgcat tatcgctgtt
    10681 ttggtaccta atattgttga agaaggcgaa actccacagg aagcctacga tttggaagcc
    10741 attatgtaca atccaaaaat cgtctctcac tctgttcaag atgctgctct tggcgaagga
    10801 gaaggttgcc tgtctgttga ccgtaacgtg cctggctatg ttgttcgcca tgcccgcgtt
    10861 actgttgact actttgacaa agatggagaa aaacaccgta tcaaactcaa aggctacaac
    10921 tccattgttg ttcagcatga aattgaccac attaacggta tcatgtttta cgatcgcatc
    10981 aatgaaaaag acccatttgc agttaaagat ggtttactga ttcttgaata aagaaaatcc
    11041 cgttgcaaga cggggttttg tgttataata gaggcatgaa aacaaatgat attgtctatg
    11101 gtgtccacgc tgttaccgaa gccctccttg caaatacagg aaacaaactc tacctccaag
    11161 aagatctccg aggtaagaat gttgagaaag tcaaggaact agctacagaa aagaaggtgt
    11221 ccatttcttg gacatcaaaa aaatctctct ctgagattac tgaaggtgct gttcatcaag
    11281 gttttgttct acgagtgtct gaatttgcct atagcgagct agattacatc cttgcaaaaa
    11341 cacgccaaga agaaaatcca cttctattga ttctagatgg tctaaccgat ccccataatc
    11401 tgggttctat cttgcgaaca gccgatgcga ccaatgtttc aggtgtcatc attcccaagc
    11461 accgtactgt cggagtaact cctgtcgttg ccaaaacagc cacaggtgct attgaacacg
    11521 ttccaattgc ccgagtgacc aacctcagtc aaaccttaga taaacttaag gatgaaggtt
    11581 tctggacctt tggaacggat atgaacggta ctccttgcca caagtggaat acaaaaggga
    11641 aaatcgccct catcattgga aatgaaggaa aaggtatctc tagcaacatc aaaaaacagg
    11701 tcgatgaaat gattaccatt ccgatgaatg gacatgttca aagccttaat gccagtgttg
    11761 ctgcggccat tctcatgtac gaagttttcc gaaatagact ataaaaaagt ttccagtcat
    11821 ctgattggaa acttttttat gattaactat gttctgtaat gaatttatag gcttcttgac
    11881 cagcgatagc tccatctcca accgctgttg ttacttggcg aaggtctttc aagcgaacat
    11941 ctccaactgc aaagataccg tcgactgcag ttttcatgtg gttatctgtc acaatccatc
    12001 ctgcctgatc ttggatattc aattctttaa caaaatcgct aagagggtcc aaaccaacat
    12061 agataaagac accaccgaag gcttgttctg tcacttgacc tgttttcaca ttttcaaata
    12121 cgactgattc tactcggttt tcacccttga tttcccttac tacagaatcc cagataaagc
    12181 tgattttttc attcgcaaag gcgcgatctt gtaaaacctt ttgggcacga agttggtcac
    12241 gacggtgaac aatggtaaca gtcttagcaa aacgagtcaa gaagagggct tcttcaacag
    12301 ctgaatctcc accaccaact accaataaat cttggtcacg gaagaaagca ccatcacaca
    12361 cagcacagta agaaacacca cgactgttca gttcttcttc tccaggcact cccaaaggac
    12421 ggtgtttaga accagttgct acgataactg tacgtgtttc atatgtttgg tcatcagtca
    12481 tcactttctt aaaatcacca tggtcttcga cattttcaac ataaccataa atgtgctcaa
    12541 caccaagatt ttcaagtggt tcaaacatct tttcagccaa ttcaggtcca ctaatattag
    12601 cgtatcctgg gtaattttcg atatcagatg tattattcat ctgaccacct ggcagaccac
    12661 cttcaatcaa agctactttt agattgcttc gagcagcata caaggccgca gtcatccctg
    12721 caggtccagc accgataata atagtatcgt acatatagat tccttctttc ttgttgtaac
    12781 tatctttatt ctaactcttt cttgtcaatc acgcaaggat tatgccttga aaacaagaat
    12841 taaactcata accgcaaagg ataatactgg gacaaccaag actccaatca taatcatatc
    12901 atagagatgg gcttggcgag ccctggctgt cttatcaact cccgacatgg ctctcagacc
    12961 aatccaaatc cctaaaaaaa tcagaacaag gatggtggtc aagatcaaac tctcgaaata
    13021 taaagaaaat agttgcagta gcatgatttc tctcatttct atctttttta aagagtaaac
    13081 tcagctagtc caactaactg agttttcctt tatctattat atcaaatata agtccgtttg
    13141 taactagcga agaattcttt tgtccgctct tctttagggg tgtggataat ctcatccgga
    13201 gttccagact cgatgatttt ccccttatct aagaagagaa ttttatccgc aacttgggct
    13261 acaaaggaca tgtcatgact gaccaaaatc atggtctgac ctgacttagc agcatctgca
    13321 atagactttt ctacttcacc gaccaattct gggtcaaggg ctgaagttgg ttcgtctaag
    13381 agcaaaacat ctggtttcat agcaagcgca cgcgctaggg caacccgttg cttctgtcca
    13441 cctgataaat ggcgaggata atggttttca cggtccgaaa gcccaacctt agccaactct
    13501 tccttggcaa tcttagtcgc ttcttggtca gataatttct tgacaacaac caagccttct
    13561 ttcacattat caagtgctgt tcggcgttca aataaattaa actgttggaa aaccatagac
    13621 aacttacggc gtagggcaag gatttcttct tgagtgattt tagaaaaatc aactgaaaaa
    13681 ccatcaatct gaatagagcc actgtcaggt gtttcaagat aattgagact gcgaagaaag
    13741 gttgattttc cagctcctga agaaccaatc aaggctacga cttctccttt ttgaatatcc
    13801 aagttcagat gatccaagac agtctgtcct gaaaaggatt tgcttaaatt cgaaatctta
    13861 atcattaacg aaggtctcct ttcacatctg tttgcactgt atcaggtgca gaaatagcca
    13921 tttttctctc gatgaaacga ccgaggcttt caattccgat attgactacc caataaacaa
    13981 gggcaacgga gatgaagcgt tcaaaatagc gataatcagc tccacctaga atctgagctt
    14041 gggcaaagac ttccacaaca cccgcactaa aagctagaga tgttcccttg gtcaaaccga
    14101 tgagggaatt aatcaaggtt ggagtagcta ccaccgctgc attaggaata atcactcgtc
    14161 gataaacttg cgctcgggtc atacccagac tgcgtgccgc ctcaatctca ccaggattaa
    14221 ctgagagaat ggctgcacga atggtttcac tagcataagc tgcctcatta aaggcaaaag
    14281 cgacaatcgc aaaagctgca gctggaatcg cattgatatt gagaccagtt ccccattgct
    14341 gattgagggc tttcaaagcc aaagggattc cgtagtaggt caacatgagt tgcaccaaaa
    14401 tcggtgtccc ttttaagaaa ctaacaaaga aggcctgcaa gggatataaa atcttgacac
    14461 gattgatctt cacaatggca aaaagaagcg ccaaaaccaa gccaaaaagg gcaccaccaa
    14521 ttgtcaacat aattgttgtt ggaagttgtt ggacaattct agggattcca tcaaagaccg
    14581 aacgtaggct aaacagcttg ccatccggaa tcaattgcat caagttttgg taccaatctg
    14641 atgctaaaat cgttgtaaca ttcataaaaa catcctcttc tgataatgat tcattctacc
    14701 atgcttctgc cgtcaatgaa attatttgct acggtcagat acagactttg tctacctact
    14761 agtttatcat actttgaaac agggaagtta tatattttat ctatcaaaga gatagataaa
    14821 aactatttca gtcccagttc ttcataagat tttcgataac cgatttgctt aacagttcca
    14881 ttttctacta aaatgggccg ttttaacaac ataccgtcac ttgctagcaa ctcagccgct
    14941 tcttggtttg acaaacttcc taccttatct tttagcccta attcacggta tttgatacca
    15001 ctggtgttga aaaattgctt caattcaaat cctgaggttt ctagccaatt caaaatgact
    15061 tcttggctag gtgtttcttc cacgatatgg acggctttat agtccacacc taattgattt
    15121 aattcttgtt ttgctttttt acaagttgaa catttggggt attcgataaa ttctaacatg
    15181 tgcttctcct tctatttgtt tatatcttta aaatctacgc cttcatgctc caagagccaa
    15241 gctttctttt ccactcctgc agcatagcct gtcagacgct tgcctgctcc caacacacga
    15301 tgacaaggta ctaggataga ccaaggattg cgtcccactg ctccaccaat tgcttgagca
    15361 gaagccactt gcaggtcttg agcaatttgt ccataggtca ctgtctgacc ataaggaatg
    15421 ccctgtaaat agtcccaaac tctcttttca aaattcgttc cgattggcgc caagagcaag
    15481 ttggataaat cctgaggctt gcctttaaag taatcatcta agcaagcaat aactgggtct
    15541 aaaataggat gactaacaac ttcttctatc gtttcatctc ctagtcccct ctcaaaatgc
    15601 ttctgctcct gaacccaaat tccatacaaa taatggtcat cagctacaag tgatagaata
    15661 ccaattggtg aggagtagag tatttttaca tacttctttt gcattatttc tttaattttt
    15721 gataggccaa gcgttcgcca tctactggca ccttaccaac ttgtttaaaa ccaagttttt
    15781 caaaaatatg ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa tcaagataat
    15841 caaaaccttc aatcaagccc tctaagaagg tttgagcaac tccttttccc tgcacatctg
    15901 ctgccacagc aatacggtga aagactagat actctgactc tccagcttgc cagtttccct
    15961 cataaatagc ttcataggcc tcctctggac tcttggtcac agcagcatag gctagcagtt
    16021 ctccctcttc caaggctacg taggcttgcc ctgagatgat atcatcgata ataacatctg
    16081 catttggata gccattttgc cactggtcac taccagcatc tgctaaacat tttttggcat
    16141 cctccatcac ctgcatgatg gcatctactt catttggaaa agctaaacga atttccatct
    16201 tttttcctca tttctgacta gtttctccca tcatagcata atttaagtat ttctacaagc
    16261 agttaaaact tgactttgtg tttttgttat tttataatct agttatcaaa actatataaa
    16321 aaggagttaa aaatgaaaac taccttttcc tacccaaaat gggcagaaat tccaaacatt
    16381 gacctctatc tggaccaggt tttgctctat gtcaatcagg tctgcgcccc tatctctcct
    16441 aataaagaca agggcctaac agcatctatg gtcaacaatt atgtgaaaaa tggttacctg
    16501 acaaagcctg acaaaaaaaa ataccaacgc caacagattg cccgtttgat tgctatcaca
    16561 accctcaagt ctgtattttc tattcaagaa atagcccaga cacttaatac tctacaaact
    16621 caagcaagtt cagaccaact ctacgatgct tttgtggact acatgaacca aggaattgac
    16681 ccagctaacc ctattatcca aaccagctgc caaaccgtta aactctatca tcaaactcta
    16741 gacttaatcg atcatactca agaggaggta atccaatgaa cactagtctt aaactcagca
    16801 aacaactcag ttttggagag gagattgcta atagcgtgac ccatgctgtg ggtgcagtca
    16861 tcatgcttat cttgctgcct atttcatcca tctatagtta tgaagcacac ggatttttat
    16921 cctctatcgg cgtttccatt ttcgtcatca gtctctttct catgttccta tcatccacca
    16981 tttatcactc tatggcctat ggttcgaccc acaaatatgt tttgcgaatc attgaccatt
    17041 ctatgattta cgttgccatt gccggatcat acacgcccgt tgtcttgacc ttgatgaata
    17101 actggtttgg ctatctgatt attgtcatcc aatggggaac gaccatcttt ggtattctct
    17161 ataaaatctt tgctaaaaag gtcaatgaga aatttagcct tgctctttac ctgattatgg
    17221 gctggttggt tctggctatc attcctgcca ttatcagtca aacgacaccc gttttctgga
    17281 gtctcatggt aactggcgga ctctgttata cagttggagc tggattttat gccaagaaaa
    17341 aaccttattt ccacatgatt tggcatctct ttatcctagc tgcgtccgca cttcaataca
    17401 tcgctattgt ttattacatg taaaaaagtt gagaaattca atctcaactt ttttctttac
    17461 acatattgat aaagtactgg tgcaagcgca catcatcagt caattctgga tgaaaagaac
    17521 ttaccaacat atttttttct tgggctgcaa caatttgatt gttcactgtt gctaaaattt
    17581 ctacaccctc accaacacta ctgataatcg gaccacggat aaaggtcatt ggaatcttgc
    17641 caactccctt acattctgct tccgtgtaga aacttcctaa ttggcgccca taagcattac
    17701 gctcgaccac catatccata gttcctagat gactctcttt ctgagaagtg atttccttag
    17761 ccagcaaaat taagcccgca caggtcccaa acactggtaa gccagataga atggcttctc
    17821 ggatgggaag tagcatgttc tggtcacgta agagcttgcc catggttgta gactcaccac
    17881 caggcaaaat caaacccgac aagtcactct gatcttgctg aaaatcatct agatttctga
    17941 gttctacact ctcgacacct aattgatcta gcacttttgc atgttctgca aaggcccctt
    18001 gcaaggccaa tattccgatt ttcatctatt ttcctcgttc agccatgaga atttggattt
    18061 cattttcatt aataccaacc atggcttctc ctaaatcttc agagatttga gctaggattt
    18121 gaggattacg gaagttagtc acagccttaa caatggcact cgctcgttta acaggatctc
    18181 ctgacttgaa aatacctgaa ccgacaaaga ccccctctgc ccctaattgc atcattaacg
    18241 cagcatctgc tggcgttgca acacctccag cagcgaaatt tacaactggc aattttccat
    18301 gttcatgaac atattggacc aattctacag ggacttgcaa atccttggca gcaacataaa
    18361 gctcgtcctc acgtaagttt tgaatgcggc gaatttcctg attcatcata cgcatatgac
    18421 gaacagcttg gacgatatcc cctgtccctg gttctccttt ggtacgaatc atggaagcac
    18481 cttcagcgat acgacgcaag gcttcaccca aatccttagc accacagaca aaaggaactt
    18541 ggaattcttt cttgtccaca tggaaacggt catcagctgg agatagaact tcactctcgt
    18601 cgatataatc aatttcaata gcctctaaaa tctgagcttc aacaaaatgc ccgattctga
    18661 ccttagccat tactggaata ctaaccgctt cttggatttc cttaatcatc tttgggtcgc
    18721 tcatgcggga aactcctcca gctgcacgaa tatcagccgg aattcgttcc aaggccatca
    18781 cagctgccgc accagcagct tctgcgatac gagcctgttc aggattctgc acatccataa
    18841 taacaccacc cttgagcatc tgtgccaagt ttttatttag ttcataacga ttttcagtca
    18901 tttgttttat cctcatcatt tgtattggta gctacctttt cattttaagt cagaacagaa
    18961 tgaagcacaa gataaaaaaa ctataattgt atggggacaa attagctaaa aactatctga
    19021 ccttaactta aggccagata gctcattcat ttttattttt cagccgtaag ggcagccatt
    19081 gtgatgtagt tgtatggttt gttgaagtgt ggcaagaaga agaggtctgt caatgccaat
    19141 ttatcaattg tcacatgctc ttggatagca agtgagaaca tgtggattcc catgctaatt
    19201 gcaatatcat gtgaaaccat ttgggcacca agaatttcac ggctatcttt gtcaaagaca
    19261 atcttaattg ctacttcatg gttgtcatgt ttcatgaatt ctggtttttg aagatcgtta
    19321 aagcctgttt cagttgcgtt gtaaccagca gctttcgctt tttcaagagt caaaccagtt
    19381 gaaaccatgt gaagaccgta gattgagata ccatttgaac cttgaacacc gattccttcc
    19441 aattcatgtc cacaagcatt gtaggcacca acgataccag tgcgaacagc atttgaagca
    19501 agagcgatat agcttgtatc tttacgagca ttgtcataaa cagtcgcaca gtcaccaaca
    19561 gcgtaaacgt ctgggataga tgtttcttgt ttcttgtcta caaggaaggc accgttgcgg
    19621 aagagttcga tcttaccacc tgcaagggct gtgtttggac ggaaaccaac tgcaaggata
    19681 accatatcca cgtcaaagct ttctttgtca gtaatcaagc gttcaacttt accgtcacct
    19741 tcgattgctt taacagtttg acctagagcc aagcggatgt tgtgatcttc caagttcttc
    19801 gccatcattt gtgtgaagtc tttgtcatag taaccgttca agacagtatc aacgatatca
    19861 acaaggacaa cttcttttcc aagacgttca aaggcttcag caagttcaac accgatgtaa
    19921 ccaccaccaa caacggcgat acggtcgagg tgttggctct tgtcagaaag tttattgata
    19981 acttcttcag cattttggta caatttcacg aattgtacgt tttcaagagt tgctttaaat
    20041 tcgcggtttc ctttaacaat ttcaacacct tcgattggtg gcaagattgg tgtagaacct
    20101 gtagcgaaaa tcaatttttc gtatgattct ttgtgctctt ttccttcaac ttccgctgta
    20161 actactttgt tatcatagtc gattgaaaga acaggtgagt tcatgtaaac tttagcacct
    20221 ttagcttcca atttttcttt atcagaatag aacaagcctt cagcaccgtc aatttgttca
    20281 ccaatccaaa gagccattcc acatcctagg aaagagatgt tagagttttg gtcaaataca
    20341 acaatttcgt tctcatttcc aaaattatcc aacatggtat tgatacatgc tgtaccagcg
    20401 tggttagcac cgactacaac gattttactc ataaaaattt cctgccttca ttctaaattt
    20461 accttactag tataccattt accgttagcg tttacaaggg gggtactatt atttcagcat
    20521 tttttatcaa aaataattcc ccatttatcc tgttttcaag cctgatgaat tttatgagca
    20581 tatttcttga ctttttccaa attttgtttg tgaaaattct aactttatgg tattataata
    20641 acatgaaaat gaaatgtaag ggcttcaatt tagatgatta tttgatatta caacttattt
    20701 tctataagag aaaggagttg gtagcttgac tcttcgttca cattctgaac ggctaatggg
    20761 gactactatc actatttcat tagtggatga gcaagccgat atctttctcc aaaaatcctt
    20821 tgacttgctc aaagaacttg aataccgctt caacgccaat agtcaagaat ctgagttgat
    20881 ggaaatcaat tatcaagcag gaatagcccc cgtcacggtt catccagacc tgtttgagct
    20941 gatttcactt gggttagagc atagcctagc gctctctagc cacctcaata tcagcattgg
    21001 tcccttaatt caaacctggc gtatcggttt ttcagatgcc aaggtcgccc agcctcaaga
    21061 aattgaatcg gtgctgcctc taatcaatcc tcatggtatc gagttagatt cttctacttc
    21121 tactgtgttt ttaaaacaga aaggaatgaa gatcgatcta ggttgtttag ccaaaggata
    21181 cagtgcggat aaggttgccc aatttcttag gaaagagggg gtgacttctg ccttgatcaa
    21241 tctgggaggg aatatcctga ccattggaaa aaatcaggca agaggggata acccatggca
    21301 aatcgggatt caagacccag ccaatcctag gggaaatcat ttaatgacca tccctgttgt
    21361 caataaatct gtcgtgactt caggcattta tgaacgtcac ctgaccgtcg atggacaaga
    21421 ttaccatcac atttttgaca gtcaaacagg atatcctgtt gaaacggaac tagcgagtct
    21481 aacaatcatc tctgataaat cagtcgatgg cgaaatctgg acgactcgac tctttggaga
    21541 aagacctgct tctatcctct ggcaagtcga aagtttggag ggcatcgaag tcatcctcat
    21601 cgataaagaa ggccacctaa gctgttcttc aggaattcct actctataga gacaactgtt
    21661 tcaatggaat tttaaaattg tattatgtaa aaagagaaag gaaatctcat gttaaaactt
    21721 attgctattg ttggaacaaa ttcaaaacgt tctacaaacc gtcaattgct tcaatacatg
    21781 caaaaacact ttactgacaa agctgaaatt gaacttgttg aaatcaaggc cattcctgtc
    21841 ttcaacaaac cagctgacaa gcaagtacct gctgaaatat tggaaattgc tgctaaaatc
    21901 gaagaggcag atggcgttat tatcggtact cctgagtatg atcactctat tccagctgtt
    21961 ttgatgagcg ctcttgcttg gttgtcttat ggtatttacc cacttttgaa caaaccaatc
    22021 atgattacag gtgcttctta cggtacgctt ggttcatctc gtgcccaatt gcaacttcgt
    22081 caaatcttga atgctcctga aatcaaggca aatgttcttc cagatgaatt cttgctctca
    22141 cactctcttc aagcatttaa cccaagtggc gacttggttg accttgatgt tatcaagaaa
    22201 ttggatgcca tctttgatga cttccgtatc tttgtaaaaa tcacagaaaa attacgtaat
    22261 gcacaagaat tacttcgcaa agatgctgaa gactttgact gggaaaattt gtaagatagg
    22321 agaccaaaaa gaatgaaatt tgttggactt gttggatcaa actacgatca atcatataac
    22381 cgtaagctct tggaatttat ccgtcgctac ttcaaactca aatttgaatt agaagttctt
    22441 gaaattgacg aagttccaat gtttaaccaa gatgaaaaat gggatgaaag tttccaatta
    22501 cgttatttat ataacaaaat tactcgtgca gatggtgtta tcattgctac tcctgagcat
    22561 aaccacacta tctcagcttc actcaaatct gtacttgaat ggctttcata cgaagttcat
    22621 ccatttgaaa acaagcctgt tatgattgtg ggtgcatcat actatgacca aggaacatca
    22681 cgtgctcaag ttcaccttcg taaaatcctt gatgctccag gtgttaatgc ctacacgctt
    22741 ccaggtaacg aattccttct tggtaaagct aaggaagctt ttgataacaa cggaaatatc
    22801 accaacgaag gcactgttaa attccttgaa acttgcttag ataactttgt taaatacgta
    22861 ggagtcgttt cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga
    22921 aaaccaatcg ctacaaccat cacagaagtt gaccctgacg atccagaatg ggtagaaaaa
    22981 gttgcagcat tcactggtgc tgtttctggt gatacctatg tcaaattgga ccacggtatc
    23041 ctaacagtta accaaattga tatgttcttg aaagctatgc catttgaatt gacatacgct
    23101 gacgacaaca accaattcct ctactacaac aacgctcacc aagatccaga cactatgttc
    23161 gctaaacgtg taccacctca atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca
    23221 ccagcacgta tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa
    23281 tacgtccgta cgatcgttcc aggttctcct gcaggtgtta tcaacactca caactaccaa
    23341 gcaatgtact atcctgatgg atcatacgct ggtatcaatg aaatcgtctt taacttccaa
    23401 ccatggcttg actggtacct aaaagagact ggtcaacgtt tggtgggtgg tagcggacca
    23461 tttgctcctg ctgccggagg tcatggagac gccgatgcta cttctagcgc ttctgattca
    23521 ggggatgctg gaggccacgg tggtgacgca gacgctacag ccggtgcaag ttactaatga
    23581 tagtttttag gaactattat ggttcctttt ttagcgaaaa aagactagca aacacaaact
    23641 gctagtctct ttgttatcgt ttcatctatc tttgttaaaa gaaagaatac atatactcaa
    23701 tgaaaatcaa agagcaaact aggaagctag ccgcaggctg tacttaagta cggcaaggtg
    23761 aagctgacgt ggtttgaatt tgattttcga agagtattat tatgaatttg gatcatcaag
    23821 actacggcca tgtaatcctt tttcacgttg tacttggcgt agtttttctg gtgtaacatc
    23881 gtttccttct tcgtccacaa ttttgattcc ttcaatgtgg tgacgaacag cgcggcgata
    23941 accttcgatg tactcctcac gtagtttggc ttgttccact ttttcttctg gtgttaagcc
    24001 ttctgttttt ttctttttag caagctcatt gatacgagca atttttttcg gatccatttc
    24061 ttctcctttg taataagata aagtccgttt atcaaatttt atttagtgtt aatttggtta
    24121 aaacaagcaa acttagctgc tttcttggtt agttgtgaca agattgccaa acgattttga
    24181 cggacagcct gatcttcagc cattaccata gtattttcaa agaaagcatc aatgactggg
    24241 ctaagcgcaa aaagttgttt caattgctga cttgcaggtc ctgataaaat gagtgtttct
    24301 actgcttctg ccaaagcttt ttcttggtca ttctcaaata gtgctgaatc aaccgtagca
    24361 accccttctg ccttctcggc caggttaaag gcacgagaaa gtgattcaac agatggttta
    24421 aaatcttctt ccttgcttac ttctacgaga gcacttgctg cttccaacat atctgccaca
    24481 acaaagtttg aacctgcaag aactgcttcc ttgatatctt ttggagtaga gcccatcatc
    24541 ttatcaacac gagccttgat aaagtccata acctctgctt tattttcata agtcaaactg
    24601 tcaaatttca atgcataaag gctatcaatc agctcatcca tagcaatgtg ccaaccaaag
    24661 gcatccaaga tacgaaccac accttgagtt gcacgacgaa gggcataagg gtcattagaa
    24721 cctgatggaa tcaatcctac tgagaagaaa ctcaaaatcg tatccaattt gtctgcaatg
    24781 gctagaactg cgccgacctt gctctctgga agttctcctt cagctgatgt aggcatgtag
    24841 tgttcacgaa tagcagctgc caccgctgga gtttcaccag caagaagggt gtatttttca
    24901 cccataattc cttggagttc gtcaaattca ccaaccatac ctgtcaacaa gtcaaacttg
    24961 taaatggctg ctgcacgagc aaggtcaact gtttcatcca ctgacaaact agctttttct
    25021 gccaaaagta cagtgatttg acccgtacga atcatgtgtt cacgaaggga accaatcttc
    25081 tcatggaagg tgacattgtt taatttttca acaagatctg aaatcaccaa tttttggtct
    25141 tcacgccaga agaattctcc gtcttccaag cgggctacca agactttttc atttcctttg
    25201 atgacatttt tcaaacgctc tgcgtttccg ttacgaacag aaatgaagtt tggcaagagt
    25261 tttccatctt gatcacgaac aacaaagtaa cgctggtgtt ccttcataga agtcaccaag
    25321 acttcttctg gaacttcaag gtatttagca tcaaaacttc ccatgaaggc agttgggtat
    25381 tcaaccaaat tcaagacttc attcagcaaa tccgcatcaa tttcgatacg tacaccatgt
    25441 tttgcctcaa tttccttgat ttggtcaaca atcatttgct cacgttcaca tggatctgcg
    25501 attacaaact gcttacgaag gtcttcttca tagctcaatg ctgactgaat cttggtttct
    25561 tgtcccaaaa aacgatggcc acgactcaca cgacttccct tgatatcaag gaaatccaag
    25621 tcaaactctt gctcatccaa gagaacagtt aaagtgtgaa cagggcggat gtattcaaag
    25681 ctatttcccg cccagtgcat gctgacaggg aaagtcagtg acttcaagac atccacaatg
    25741 cctggaacaa tggcttcaac tgcttgacca atttcttcct tagtgacata gacatattct
    25801 tcacccttga tttcacggaa ttcgatatct tcaacagtca aacctttccc acggacaaat
    25861 ccttgagctg ctttggtgaa gtttccatca ctatctaagg caattttctt tgctggaccc
    25921 ttgaaatctt ctgttaaatc agactgtttg tctgcaagac cagttacacg aacagccaaa
    25981 cgacgtggtg ttgagaaagt ttgaatggct tcaaaagaca ggcgttttcc cttgaggaag
    26041 gctgccattt tttcgcctag ttgtttttca cttggcgtaa caacataggc tggtaattct
    26101 tcaagaccga gttctactaa taagtttttt gtcatgtttt ttcctttaca ttttcttcaa
    26161 tcttttttga tatgcacctt aggtactggg gacgtcgcta actaactctg tgagtctgta
    26221 tggaaatcca atgctaaatt tatcaagatt tccaacaatc tcatcaaagt ggatttagct
    26281 gactgatttt tgaacctaag gtttcaaaaa tccccacata acagtacagc ggtgcatatc
    26341 cctctagcaa gtcttcgact tgcctctaac gattttagag tacagtatta ctcactattc
    26401 tgcgtcttct gctaaaagtt taactcgtgt ttcttcatcc aaaagtgggt aacctaggcg
    26461 tttgcgttct gcaacaaagg ttttggctac aacacgggcc aagttacgga tacgagcgat
    26521 atagcctgca cgctctgtta cagatacggc accacgcgcg tcaagcagat taaaggtatg
    26581 tgaacatttg agaacatagt cataggcagg gtgtaccaag ccttcttcta atgcacgacc
    26641 agcttctttt tcaaacttat caaaattttc aagcaacatt tcttggtccg aaatttcaaa
    26701 tgaatatttt gagtgctcat actcaggctg gataaagatt tctccgtatt ttacaccatc
    26761 agcccactcg atatcataga cagagtctac ttcttgaatg taagaagcca agcgctccaa
    26821 accataggta acttccgcag tcacagggct agttgccaat ccaccgactt gttggaaata
    26881 agtgaactga gtgatttcca ttccgtcaag ccaaacttcc caaccaagac cagctgaacc
    26941 agttgatggg ttttcccagt tgtcctcaac aaaacgaata tcgtgctcca aaggattgat
    27001 tcccaatttt tccaaagact caaggtaaag ttcttggata tttgatggag aaggcttcat
    27061 gaccacctgg aattggtggt gttggtagag acggttaggg ttttccccat aacgaccgtc
    27121 agcaggacga cgtgatggct ctacataagc tgcattccat ggctcaggtc cgatagcacg
    27181 aaggaaagtg taaggactca ttgttcccgc acctttttca ttatcataag cctgcataag
    27241 catacaacct tggtcattcc aaaattgttg caaagtaagg atgatttcct ggaaagtcaa
    27301 tttcttagac attgtttact cctttagttt ttatatttct ttttcaatta atttctgctg
    27361 aacaaaccaa tctaacaagg gaaaagtttt ttctgtattt gcccataaat gataagaatc
    27421 taagaccgct tgtacactac ctaaattttc taccaactta tctactgtca aaaagctgcg
    27481 ccagtattca taaaaaatac ttgcatagtt cttatcataa gtcgaagaac caaaatcatt
    27541 caatgaatgc caaccatact tcttctgaaa aagttctacg agagattgat tacaaatttt
    27601 ttccgcttga aattcctctt ctgtcaagaa atacttgcga ctaatatatt caaccatccc
    27661 ctcttcgaac cagatataag agtcataacc atcaaaatca tctaaaaaat gctccgacca
    27721 atgagctaac tcatgtccta caatctgtaa gaggaaattt tcagataaag aatgatagtg
    27781 actttctatt gcttgagttt gctcagaaca ctcgtagtcc atcaattgat acaaatacaa
    27841 ctctttccaa acagctaaat caggcgccat aaccattcgt ctattatttg tataggctgg
    27901 aacagcgctt tccctaataa tctgtgtagc agcattaaaa tctgaccaaa taacggcttg
    27961 cggtaaatca cagactgcaa gttcattctt taaaaaagat tgataatctt tcaacttttc
    28021 agtatttctt actacaaaat cacgaaatgt agctagttga ctatcgtctt ttacaagata
    28081 aaggttgtcc acggctctcc tttctttcac acaatagact gaataagcaa aagaaccaca
    28141 tctgacaatc ctaggttgat ttaacctagg ggcgtcagaa acgcggttcc accctaattt
    28201 attacttcat tgtttttgaa aatactacag aaagcgccag ctcattattt tgccctactt
    28261 ggctcccact gtcccaagct cgcttgaaga acgccataag actttctttc ctgctgacta
    28321 ttatatcaaa aattagaata atgtacaatt ctttttatga ttttctagaa atccacatca
    28381 tcaactcgtg gagcccccga ctgaacagca gtcgttttta aggtttctct ctcctcatga
    28441 ctcagttcaa ttccaaaaca atcaagatta gcttgaatac gagaggttgt gacagatttt
    28501 ggaagtggta aaaatccttc tgccaagctc caggccaagg ctatctgagc aaccgatttt
    28561 ccgtgatttg ctgcgatttc ttggacttgc ttgctatcaa acagttctcc ttgtccaaaa
    28621 ggcccccaag cttccaataa aattcccttt tcacgacagt aagctacgac ttcctcttga
    28681 tacacacctg gcgccaagcg aacttgattg accgcaggaa cgatagttgc agtttcaagc
    28741 aaggcatcca aatgatgggg aagaaaattg ctaacgccga tagcacggat tttcccttct
    28801 tgatagaggt cttccatcgc tctccaaact tccgcattgc gagttttcca tgcgtcattt
    28861 tctctgagcg gttttgggtt cggccaatga atcaaataca aatccaaata atccaagccc
    28921 agtttttcta tagatttttc caaagcttga cgagtttgct cataggtttg ctgactattc
    28981 caaagcttgg tagttacgaa catttcttca cgtggaactc cgctatcttt gattgcttga
    29041 ccaacacttt cttcattctg ataaatcgcc gccgtatcaa tatgacgata accagccttc
    29101 aaggattcta acactgcacg ataggcttct tctccatcct tagccttaaa agttccaaat
    29161 cccaatactg gaatttctac tccattattt aattgatagg tattcatatg cttctccttc
    29221 tatatttatt catcaattat agcataaaac attttttaag tagtactatc ttctgtaaaa
    29281 aataagtctg tcaaatatct aacagaccta ttttttcttg cttattcatc acgactatag
    29341 tacagctcat gagcttttag gcgagtattg agcatctctg gtatggtcac aaaggtataa
    29401 ccttgatttt tcaaatactc aatgaccctt ggcaaggcat tgactgtcgg actgtgaata
    29461 tcatgcatca aaacgataga gccgttagct acttgatgct gaatttctgt caaaatagat
    29521 gcttcatttt tactcttcca gtccagacta tccacatccc acatgataaa gctcaaatcc
    29581 aagctattgc gaatatcatc tgtaatagca ccataaggtg gacgcatgag tttagaacta
    29641 gaacccagca ctttagttag cacatcctca gtatcagtaa tctgcttttt agcttcatca
    29701 agagagagtt gcgagagaat cggatggctc caactatggt ttccaacaac atgaccttca
    29761 gattttatcc tcttcaccaa gtcctcattc ccagaaacat ttttcccaag cacaaagaaa
    29821 gtcgctttaa tatcatattt agctagggtc tctaatacct gcggggtcgt tgctggattt
    29881 ggaccatcat caaaggttag agcgacaact ttttgatatt tcttatcaaa gtaagattgg
    29941 tacaaggccg catctttttc gagtaagtac gaagattgga taacatcaaa gaaagcagat
    30001 actggcaagg ctatctcttc taaattttca accacaggac ttggataaag gataatctga
    30061 ctatccttgt aatcaaaatt ccatgcagac aagtcttggt cagagaagtt ttttacaatc
    30121 tgctcactct ggtcttgctc tattttttta tcctctatga aggaggtcaa ctcttttatc
    30181 agctgttcct tagccttact agcatctgaa aacagttggt caagtgtaaa aggttgccca
    30241 tcttctgtta agtgaacctt tcctagactg gtcttttcag tctcttcaac cttaaaagca
    30301 actaaatcat agatttgttt ggtcacatta cgattaacga ctccctttaa acctgactct
    30361 tcttgctctg tatagtagaa aacaagattg tccttacttt ctagcttgtc cttaacatct
    30421 tgatttatca actccctaac agaggaaatc actttctccc cttggagagg ataataggca
    30481 atcacttcgg cttgcccctg acgaaaatgc tccttctgat ttccctcact caattgatca
    30541 tctttctctt ttttgagcga ttcaatcttt tgctcaaaac ttttttgctg gtagatctta
    30601 aaagcaataa atccgcctaa taggcaaata gaaattgcta aaataccaat caaagccaat
    30661 aatacatgtc tcgttttccc gtgtctgcca cgtcctagtc tacttttatt catatttata
    30721 tcataacaaa tttgagcaat aatttcaatg atacaatttg gcaatccaca attcctctag
    30781 caagccaaag catcttacat caactccaac atccgtatct gtagagatta tgtagttttc
    30841 tatctatgga atttctagta gattgaagct agaatagtag acctatactt ctaaaacatt
    30901 gttagaaatc gatttgattg tcctgatcga tttgtcctgt tctcatttca ttttactata
    30961 aaactatgaa aagctaggtc atctgagaag taattactat cgacaattgc gatagcgtat
    31021 actggtgcga tttgtttgta gctctggtgg gtatcacctt cacgttggcg aattttttct
    31081 aggttttgat taacctgact gcacagataa gcccataggc gattgatgaa aaaattctga
    31141 tgatgaacct gaatttcgat gataacttgg attccattat ctaactccgc caagacgtct
    31201 atactagtat agaaatcctg cgctgagtag ggcatggaag gcaagacgtg aatgttactt
    31261 ccctccaaaa tcgtcacatt ttttgctggc aagtccagca tatcgcggat aaattgacaa
    31321 atcattagtt gggctgatac ccggatgtct gagaatcctc cttttcctcc ttctattata
    31381 gcacattttt aaatctaggt ttgctagatt ctatgcttct atctatttat tcggaaaaga
    31441 aagctaatag cttatttgaa attttctcct ctctgttgga tagtcccgtc tatctatgtt
    31501 ataatgaaag aaggatttaa aatcggaaaa ggagtattta tgctaaaatt aggtgtcatc
    31561 ggaactggcg ctatcagcca tcatttcata gaagcagccc ataccagtgg agaataccaa
    31621 ctggtcgcaa tctattctag aaaactagaa actgcagcaa cctttgcttc tcgctatcag
    31681 aatatccaac tattcgatca attagaagtc ttcttcaaat cttcttttga tttagtctat
    31741 atcgccagtc caaactcctt acattttgct caggcaaaag ctgccttgtc tgctggtaaa
    31801 catgttattc tcgaaaagcc agctgtcact caaccacaag aatggtttga tttgattcaa
    31861 acagctgaaa aaaataactg ttttatcttt gaagcagctc gtaattacca cgagaaagcc
    31921 tttactacta tcaaaaactt tttagcagat aagcaagtgt taggagcaga tttcaactat
    31981 gccaaatact cttccaagat gcctgacttg ttgactggac aaacaccaaa tgtcttttca
    32041 aaccgttttg ctggtggagc cctcatggat ttggggattt atcccctcta tgctgctgtt
    32101 cgtctttttg gaaaagccaa tgacgcgact taccatgctc aacagcttga caatagcatt
    32161 gacctaaatg gagatggtat cctcttctac ccagactatc aagttcacat caaggctgga
    32221 aaaaacatca cttccaatct tccttgcgag atttatacaa cagatggaac cttgactctc
    32281 aacacgattg agcatattcg ctcagctatt tttaccgacc accaaggaaa tcaagtccag
    32341 cttcctatcc aacaggctcc tcatacgatg actgaggaag tcgctgcatt tgcacacatg
    32401 atccagcaac cagatctgaa tctctaccaa acttggctgt atgatgcagg ctctgttcat
    32461 gagctactat ataccatgcg ccagactgct ggcattagat ttgaggcaga aaaatgaaaa
    32521 ccaaactacc gactgaatgg caagaactga gtgaccaact cggtttccaa gaattcaccc
    32581 ccattcaaac tcaactattt gagccccttc ttgctggaga aaacctccta ggagtgagcc
    32641 aaaccggaac tggtaagacc ctagcgtatc tcctaccaag tcttctcaga ctacaaaaga
    32701 aaaaagccca acaactcttg attctagcac caaacacgga acttgctgga cagatttttg
    32761 atgtatgtaa aacgtgggca gaagctatcg gattgactgc tcaactcttc ctttcaggtt
    32821 caagtcagaa acgacaaatt gaacgcctta aaaaggggcc agaaattctg attggaactc
    32881 ctggccgtat ctttgaactc attaaactta aaaaaatcaa gatgatgaat gtggaaacca
    32941 tcatcctgga tgaatttgac caattactag atgattctca gattcacttt gtagaaaaaa
    33001 tcactcacta cgcacctcgt gaccaccaac tggtctacat gagtgccacg accaagtttg
    33061 accaagaaaa gattgtgccg aatacacgca ctattaatct ctctgaccaa aaattggaca
    33121 atatccagca tttctacatg caggtagacc aacgtcatcg agtggatatg ctacgaaaac
    33181 tggctcatgt agaggatttc cgtggtctag tcttcttcaa tagcctgtca gaccttggga
    33241 atgccgagga aaaactacag tatcgtgata tattgactgt ttccctcgct agtgatgtta
    33301 atgttaaatt tagaaaaatc atcttagaaa agtttaaaga taaccaacta accctgcttc
    33361 ttgcaactga ccttttggct cgtggaattg atatcgatag cctagaatgt gtcgtaaact
    33421 ttgatattcc tagagatagc gaaacctaca cacaccgtgc tggccgtact ggccgtatgg
    33481 gcaaggaagg atatgttatt actctcgtca ctcatccaga agaaatcaaa aaacttaaaa
    33541 agtttgcaag tatccgagaa attgtcctaa aaaaccaaga actctatatc aaataaagcc
    33601 ccattgctct atacacatcc agtgtatgga gcttttttat aagcaattca attttatact
    33661 catctgcttt caaaaagcat tctattccat ctccgattaa cgatggactt tatcacctcc
    33721 ttctccagtc cttgtatgac atcttggagt tgattcatga catcttccaa agttcgaaag
    33781 gctttattct taaatccacg tttacgaatc tctttccaca cttgttcaat gggttcatct
    33841 ctggtgtgta tggaggaata aaggtaaaat caatattagt cggaatcttt aaggtacttg
    33901 atttatgcca tatagcattg tccataacga gtaaaaaata atcatctgga taagcttgtg
    33961 aaagctcttc taaaaagacg ttcatccact cagtattaca tccaccagct attaagaaaa
    34021 atgattcgcc tgtatggggc atcaacagct ccataacaat agcgaaattc tcgtatatag
    34081 tgactatgga catgtggacc tactcctatt ggagaccaac aagatcccag tttactgatt
    34141 ctaccgaaac cagcctcatc ttggtacatc aagcgaacct tatgaaaagg tctactggtt
    34201 ttaaagcgct ttcttgtctt cttgaattga gattttattt ttagacgcga caatggtttg
    34261 agcgtctgct ttcctaggat gttctggacg tggcataata tttcgccaac catggtgctt
    34321 caacagttga tagaaggcat cacgtgtgta ggaacgatat aagcctgaaa taaggtattg
    34381 taacaaattc tcctgcctct gtagccttca aatggcgggc aagaaaggct ttctcttcct
    34441 caactgtcat atatgcatgg ttacgaccac cacgtgtttc ttgaaggaga gagtcgagtc
    34501 cgaactcctc atattttttt acatttcgcc aaatcgttgt ttgattacag tctaaaagat
    34561 ctataatctc tttataagat ttgcccatca gacgaaatag aacgatttga aggcgtttat
    34621 gatatttagc tgtacgaaag tcttttaaaa gtgttttgat ggtttggatt tcttctttag
    34681 ttgatttcat attactattg tataatgctt tttgatttga gtctagtatt atactcttcg
    34741 aaaatctctt caaaccgcgt cagcttcgcc ttgtcgtata gaatgttact gacttcgtca
    34801 gttctatcta caacctaaaa acagtgtttt gagcagtctg cagctaactt tctataacaa
    34861 aatgaaataa gaacaagaca aatcaatcaa gacaatcaaa tcgatttcta acaatgtttt
    34921 agaagtagat gtgtactatt ccagtttcaa tatattatag tttgctcttt gattttcatt
    34981 gagtattagt tcaatatcta aaaaaagag
//
LOCUS       DAVKFH010000017        34701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKFH010000017 DAVKFH010000000
VERSION     DAVKFH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.017"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            799..1020
                     /locus_tag="VW006_000895"
     CDS             799..1020
                     /locus_tag="VW006_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379772.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1537..1790
                     /locus_tag="VW006_000896"
                     /pseudo
     CDS             1537..1790
                     /locus_tag="VW006_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1918..3147
                     /locus_tag="VW006_000897"
     CDS             1918..3147
                     /locus_tag="VW006_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9379773.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3167..5863
                     /gene="ppc"
                     /locus_tag="VW006_000898"
     CDS             3167..5863
                     /gene="ppc"
                     /locus_tag="VW006_000898"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9379774.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5946..6163
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6276..7280
                     /locus_tag="VW006_000899"
     CDS             6276..7280
                     /locus_tag="VW006_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9379775.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7349..8215
                     /locus_tag="VW006_000900"
     CDS             7349..8215
                     /locus_tag="VW006_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379776.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8212..8970
                     /locus_tag="VW006_000901"
     CDS             8212..8970
                     /locus_tag="VW006_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379777.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9060..10820
                     /gene="dnaG"
                     /locus_tag="VW006_000902"
     CDS             9060..10820
                     /gene="dnaG"
                     /locus_tag="VW006_000902"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9379778.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10823..11932
                     /gene="rpoD"
                     /locus_tag="VW006_000903"
     CDS             10823..11932
                     /gene="rpoD"
                     /locus_tag="VW006_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9379779.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11946..12275
                     /locus_tag="VW006_000904"
     CDS             11946..12275
                     /locus_tag="VW006_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9379780.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12390..13433
                     /gene="cpoA"
                     /locus_tag="VW006_000905"
     CDS             12390..13433
                     /gene="cpoA"
                     /locus_tag="VW006_000905"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9379781.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13449..14774
                     /locus_tag="VW006_000906"
     CDS             13449..14774
                     /locus_tag="VW006_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9379782.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14805..14981
                     /locus_tag="VW006_000907"
     CDS             14805..14981
                     /locus_tag="VW006_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9379783.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15040..15174
                     /locus_tag="VW006_000908"
     CDS             15040..15174
                     /locus_tag="VW006_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9379784.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15238..16548
                     /gene="obgE"
                     /locus_tag="VW006_000909"
     CDS             15238..16548
                     /gene="obgE"
                     /locus_tag="VW006_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9379785.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16558..16716
                     /locus_tag="VW006_000910"
     CDS             16558..16716
                     /locus_tag="VW006_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379786.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17803..19062
                     /locus_tag="VW006_000911"
     CDS             17803..19062
                     /locus_tag="VW006_000911"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9379787.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            19078..19629
                     /locus_tag="VW006_000912"
     CDS             19078..19629
                     /locus_tag="VW006_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9379788.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19622..20899
                     /locus_tag="VW006_000913"
     CDS             19622..20899
                     /locus_tag="VW006_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9379789.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20915..21775
                     /locus_tag="VW006_000914"
     CDS             20915..21775
                     /locus_tag="VW006_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9379790.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            22156..22958
                     /locus_tag="VW006_000915"
                     /pseudo
     CDS             22156..22958
                     /locus_tag="VW006_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            23149..25440
                     /gene="pcrA"
                     /locus_tag="VW006_000916"
     CDS             23149..25440
                     /gene="pcrA"
                     /locus_tag="VW006_000916"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9379791.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            25483..26163
                     /gene="radC"
                     /locus_tag="VW006_000917"
     CDS             25483..26163
                     /gene="radC"
                     /locus_tag="VW006_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9379792.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(26160..26849)
                     /locus_tag="VW006_000918"
     CDS             complement(26160..26849)
                     /locus_tag="VW006_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9379793.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(26867..27508)
                     /locus_tag="VW006_000919"
     CDS             complement(26867..27508)
                     /locus_tag="VW006_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9379794.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(27591..27806)
                     /locus_tag="VW006_000920"
     CDS             complement(27591..27806)
                     /locus_tag="VW006_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9379795.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(27814..28161)
                     /locus_tag="VW006_000921"
     CDS             complement(27814..28161)
                     /locus_tag="VW006_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9379796.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(28166..29281)
                     /locus_tag="VW006_000922"
     CDS             complement(28166..29281)
                     /locus_tag="VW006_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9379797.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(29291..30250)
                     /locus_tag="VW006_000923"
     CDS             complement(29291..30250)
                     /locus_tag="VW006_000923"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9379798.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(30363..30932)
                     /locus_tag="VW006_000924"
     CDS             complement(30363..30932)
                     /locus_tag="VW006_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9379799.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            31060..31731
                     /locus_tag="VW006_000925"
     CDS             31060..31731
                     /locus_tag="VW006_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9379800.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            31715..32533
                     /locus_tag="VW006_000926"
     CDS             31715..32533
                     /locus_tag="VW006_000926"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9379801.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            32530..33426
                     /locus_tag="VW006_000927"
     CDS             32530..33426
                     /locus_tag="VW006_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9379802.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            33470..34444
                     /gene="pta"
                     /locus_tag="VW006_000928"
     CDS             33470..34444
                     /gene="pta"
                     /locus_tag="VW006_000928"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9379803.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT        10487 a         6187 c         7613 g        10414 t
ORIGIN      
        1 ctatctttat gcaaatttgc tgtataagat ttgattccat tttagcaaag gccttaccta
       61 atattttgct gatacttatt gttttaatca cgtttcagag tgattggaat atatttatac
      121 ctgcaactta ttattataca gaaattcagt caactacaaa cttaggtatt aaattattag
      181 tttgtatctg gattatggga tttgaatttt tccttcttcc aaaactaata aaattgaaag
      241 aacaaaatct agtttaaatg ttgctcgaaa attaaataat tgaatacgat taacaatgaa
      301 acaatacaac gtgagtcatg acttcaaagt aattgatttt atgattctgg tttcttacgc
      361 ttaattaagc aagttgtatt cactttatca aattgttatt actctaaaaa gcgtctaaca
      421 gctatagagt gtctgtttgc ttctctttac ttttatttta aataattcat taaccttccg
      481 taagtgccca ataataaggt gtgttgaaga agtctgagat aaaatagttc tcagtcacaa
      541 aaaagctata gattcccatt ggtgaacagc tccctgtcaa gtagacagta aaataatata
      601 aaatcttaag cgacctgatt cctgaattcc agtggagtca ggttgttttt taaccgtctt
      661 tcttaatatt gtcttaccta taatagatat gcttatgaga tatgtttaat aaattaattt
      721 tagttcaaaa tgtccagtta ggacactttg aatgatttct tagttttata tattatactt
      781 ttctcaggag aaagctagat gtacagacgt ttgagagatt tgagggaaga tcacgatttg
      841 acccaaaaac aaatagctaa aatactttcg tttacaaact cagcttatgc taaaattgaa
      901 cggggtgagc atacgttaac agcagatgta ttggtaactc tctcaaactt ttacgatgtc
      961 agtacagact acctattgga attgacagat tttcctgata aaattcgctt tagaaaataa
     1021 tctcctcaat ttcatagagt gtgaaaatga ggagattttt tatttgttct ttgaaaatta
     1081 aatagtccaa aatggtactt tcctcatttg tggagcaaat ttgaatggct cgccatgata
     1141 agagcgattt taaaaccatc aataaaatag agcgatactt tatatgccat gatacaaatg
     1201 atatacaatg atacttctga ccgttcaggc tgccatcgta aaagagcagc aagtgaaatt
     1261 cttatgatga cttcatcagt catgccacgt taaatgtgtg agtttgttag agaaacgcaa
     1321 ttaatcctca aaaggttccc cgaacctttt gagttccaca gacgcatcac gtggagtgtg
     1381 taagcttgtt gctaaaagcg taaaaacctt ggaacgaaag gaataataga cttcctgcga
     1441 aacaaaaata caatacaata aaactatgga tgatgaagca agtaaacaat taagcgatag
     1501 ccgtttcaag agccttgtag gtgttcagcg cacgagatgt tagctgtgtt aaaaacagct
     1561 tatcaacgca acgtaaacgc gcaaaaggtg gacgaaaaac caaattaagc ctagacgatc
     1621 tcctcatggt aactattcaa tacatgcgag aatagcgcac ttatgaacaa attgcggctg
     1681 attttggcat tcacgaaagc aacttaatcc gtcggagcca atgggttgaa gtaactcttg
     1741 ttcaaaatgg ttttacgatt tcaaattctg ccctaattat gaaaaacagt aaaattcgaa
     1801 ggattgtaag gtaagagttt ttttctttct gaaaaaatgg tataatagca atcaaaacta
     1861 gaaaataaaa cggaatttgg aacagatttg tctgtatcct agtagagtgg tgatgctatg
     1921 aagattagta agaggcactt attaaattat tccatcttga ttccctactt gcttttatct
     1981 attttgggct tgattgtggt ctattcgacc accagtgcta ttttaattga agaaggcaag
     2041 agcgccttgc agttggttcg aaaccaagga atcttttgga ttgttagttt gatactgatt
     2101 gccttaattt ataaattgag actagatttt ttgagaaatg agcgactaat cattttagtt
     2161 atattaatag aaatgctttt attgttcttg gctcgtttta ttggtatttc cgtaaacggg
     2221 gcatacggtt ggatttcggt tgcaggagta actattcagc cagctgagta cttaaaaatc
     2281 attattattt ggtatttagc tcaccgattc tccaaacagc aagaagaaat agctacttat
     2341 gattttcaag ttttgactca aaatcaatgg cttccccgtg cttttaatga ttggcgattc
     2401 gttctcctag ttctgattgg aagtttggga attttccctg atttaggaaa tgcgactatt
     2461 ttagtcttgg tttccttgat tatgtataca gttagtggaa tcgcttatcg ctggttttca
     2521 accattctgg cgctcgtatc tgccgcttct gtctttgtct tgaccactat cagcctaatc
     2581 ggtgttgaga ccttttcaaa aattccagta tttggctatg tagccaagcg ctttagtgcc
     2641 ttttttaatc cttttgccga tcgtgctgat gcaggtcacc agttagctaa ttcttatttt
     2701 gccatggtca atggtggttg gtttggtcta ggtcttggaa actcgattga aaaacgaggt
     2761 tatttgccag aagctcatac agactttgtc ttttctatcg tgattgaaga atttggcttt
     2821 gctggtgcca gtcttatttt agctctcttg tttttcatga ttttgcggat tatcttggtc
     2881 ggtattcgag cggagaatcc tttcaatgcc atggttgcac tcggtgtcgg agggatgatg
     2941 ttggttcagg tatttgtcaa tatcggaggg atttcgggct tgattccatc tacaggagta
     3001 accttcccct tcttatccca gggtggaaat agtcttctag tcttatcagt ggcagtagcc
     3061 tttgtcttaa atattgatgc cagtgaaaaa cgcgctaagt tgtaccgaga attggaaaat
     3121 caaccaatga accttctgtt gaagtaggat aaagaaagga tagtttatgt ctcttcaaaa
     3181 attagaaaat tatagtaata aaagtgttgt gcaagaagaa gtcttgattc taacagaatt
     3241 actggaagat attactaaaa atatgcttgc cccagagacc tttgaaaaaa taatacagtt
     3301 gaaagaatta tcaacgcagg aagattatca aggtctaaac cgtctagtga ctagcttatc
     3361 aaatgatgaa atggtctata tttcacgcta tttctctatc ttacctcttt tgatcaatat
     3421 ttcagaggat gtggatttag cttatgaaat caatcatcaa aataatattg atcaggacta
     3481 tttaggtaaa ttatctacaa cgattaaatt ggtagcagaa aaggaaaatg ccgttgagat
     3541 cctagaacac ttgaatgttg tccctgtttt gacagcccat ccaacacaag tgcaacgcaa
     3601 aagtatgttg gatttaacaa atcatattca tagtcttttg cgtaaatacc gtgatgttaa
     3661 gttggggttg atcaataaag ataaatggta caatgatttg cgtcgttaca tcgaaattat
     3721 catgcagaca gacatgattc gtgagaaaaa attaaaagtg actaacgaaa tcacgaatgc
     3781 tatggaatat tataacagct cctttttgaa agctgtacct catttgacga cggagtataa
     3841 gcgcttagcg caagcgcatg gtctgaattt aaaacaggct aaaccaatca ccatgggtat
     3901 gtggataggt ggtgaccgtg atggaaatcc atttgttaca gcagagacct tgaagcagtc
     3961 tgcactcact caatgtgaag tcatcatgaa ctactatgat aaaaagattt accaacttta
     4021 tcgtgaattt tctctttcaa ctagcattgt caacgtcagc aagcaagtca gagaaatggc
     4081 tcgtcaatcc aaggataact cgatttaccg cgaaaaagag ctttaccgtc gtgccttgtt
     4141 tgatattcaa tcaaaaattc aggcaactaa aacctatctg attgaggatg aagaagttgg
     4201 gactcgttat gaaaccgcca atgatttcta caaggatttg attgccattc gagattctct
     4261 actagaaaat aagggtgagt ccttgatttc aggtgatttt gtggaattat tgcaggcagt
     4321 agagatattt ggtttttacc tagcatcaat tgatatgcga caagactcta gcgtctatga
     4381 agcctgtgtg gcagaactct tgaaatcagc aggaattcat tctcgttata gcgagttgag
     4441 cgaagaagaa aagtgtgacc ttctcttgaa agaattagaa gaagatcccc gaattctttc
     4501 tgcgactcac gcagaaaaat cagaattatt agcaaaagaa ttagctattt ttaagacggc
     4561 tcgtgttttg aaagataagt tgggagatga tgtcatccgt cagaccatca tttcacatgc
     4621 aaccagcctt tctgatatgc tagaattagc tattctgtta aaagaagtag gactggtgga
     4681 tacggaaagg gcgcgtgttc agattgttcc cctttttgaa acaattgaag acttggatca
     4741 ttcagaagaa acaatgagaa aatatctttc tcttagcctt gccaaaaaat ggattgactc
     4801 acgaaataac taccaagaaa tcatgcttgg ctactctgac agtaataaag atggcggtta
     4861 cttgtcatca tgttggaccc tctacaaggc tcaacaacaa ttgactgcta ttggagatga
     4921 atttggcgtt aaggttacct tcttccatgg tcgtggtggt actgtcggtc gtggtggtgg
     4981 gccaacctat gaagccatta catctcaacc gctcaagtct atcaaggatc gtatccgctt
     5041 gacggagcag ggtgaagtaa ttgggaataa atacggtaac aaagacgccg cttactataa
     5101 ccttgaaatg ctagtatcgg cagctattaa ccgtatgatt actcagaaga agagcgatac
     5161 caatacccca aatcgttatg aagccattat ggatcaagta gtggaccgta gttacgatat
     5221 ctaccgtgat ttggtctttg gtaatgagca tttctatgat tatttcttcg agtcaagtcc
     5281 aatcaaggct atttcaagtt ttaatattgg ttctcgtcca gccgctcgta agactattac
     5341 tgaaatcggt ggtttgcgtg ccatcccttg ggtattctca tggtcacaga gtcgtgttat
     5401 gttccctgga tggtacgggg ttggttcaag cttcaaggaa tttatcaata aaaatccaga
     5461 gaatattgct atcttacgag atatgtacca aaattggcct ttcttccaat cgcttctttc
     5521 aaatgttgat atggttttgt caaaatcaaa tatgaatatt gcttttgaat atgctaaact
     5581 ttgtgaagac gagcaagtta aggccatcta tgagactatt ttaaatgaat ggcaagttac
     5641 taagaacgtt atcttggcta ttgaaggaca tgacgaactc ttagctgaca atccatatct
     5701 aaaagctagt ctggattacc gtatgcctta ctttaatatt ctcaactata ttcagttaga
     5761 gttgattaaa cgccaacgtc gtggagaatt gtccagtgat caagaacgat tgattcatat
     5821 caccatcaac ggaattgcga caggattgcg taattcaggt tgataatttt caagagtgaa
     5881 tgctaaaagt gaatatcaaa aaaattctaa tagactattg acaagtagtt taaaaatgat
     5941 ataatttaac cattcagaaa agtaatcata caaacttttt agagagtctg tggtagctga
     6001 aaacagataa gtggcgatga tgaaaattgg gctgaatgct atttagaatt tgaaattata
     6061 aaaattcggt aagcacacct tacagtgcat ctcgttattg cgagactgag cgatagggaa
     6121 attccctata attgaggtgg taccgcgcat cgacgtcctc acacaagttt tttgtgtgag
     6181 gatttttttg atggaggtta gtatggaaag aaaacgatgg cgtcgcttgt ttagataagt
     6241 gaaatatgtt aaaggaaata aaaaggagaa acagaatgaa aaataaacgt ttaattggaa
     6301 ttattgctgc attagcagtc ttagtagcag gaagcttgat ttattcttca atgaataaat
     6361 cagaagctca gaataataag gatgagaaga aaataactaa gattggtgtg cttcaatttg
     6421 tgagccatcc atcccttgat ttgatttata aagggatcca agatggactt gcagaagaag
     6481 gatataaaga tgatcaagtt aaaattgatt ttatgaactc agaaggtgac caaagtaagg
     6541 ttgcgacaat gagtaaacaa ttggttgcaa atgggaatga ccttgtggtt ggtatcgcaa
     6601 caccagcagc ccaagggttg gctagtgcaa caaaagacct accggttatc atggccgcta
     6661 ttacagaccc aattggtgct aacttggtta aagatttgaa aaaaccaggt ggcaacatta
     6721 caggggtatc tgaccacaat ccagctcaac aacaagttga actcatcaag gctctgacac
     6781 cgaatgtgaa aacaatcgga gctctttact caagtagcga agacaattca aaaacacagg
     6841 tcgaagaatt taaggcttat gctgaaaaag caggtctgac agtggaaaca tttgcagttc
     6901 cttcaacaaa tgaaattgcc tcaactgtca ctgttatgac tagcaaggta gatgctattt
     6961 gggttccaat tgataacacc attgcatcag gatttccaac ggttgtctct agcaatcaaa
     7021 gttctaagaa accaatttat cccagtgcga cagctatggt agaagtaggt ggtttggcat
     7081 cagttgtaat tgaccaacat gaccttggtg tggcaacagg taaaatgatt gtgcaagtct
     7141 tgaaaggtgc aaaaccagcc gataccccag tcaatgtctt ttcaactggt aagtcagtca
     7201 tcaataaaaa aatagcacaa gaactaggta ttactattcc tgagtctgtt ctcaaagaag
     7261 caggacaagt catcgaataa ataaggtagg gaggaggaga ctcttcccat tttttaaaaa
     7321 gatgaatata gaaaggatta aataaaatat gattgtttcc attatttctc aaggatttgt
     7381 ctgggctatt ctaggtctgg gaatctttat gacatttagg attttaaact ttccagatat
     7441 gacgacagaa ggttccttcc ctcttggggg agctgttgct gtcactttga taaccaaagg
     7501 cgtgaaccca tttttagcga cacttgttgc tgtaggagca ggttgtttgg ctggaatggc
     7561 agcaggcctt ctttatacaa aagggaagat cccaaccttg ctatcaggga ttttggtgat
     7621 gacttcttgt cactcaatca tgctcttgat tatgggacgt gcgaatttag gcctgcttgg
     7681 aaccaagcaa attcaggatg ttttgccttt tgattcggat ttgaatcaac tcttgacagg
     7741 tctcatcttt gtgagtattg ttattgctct catgctcttt ttcttggaca ctaaactcgg
     7801 acaagcctat attgctacag gggataatcc tgatatggct agaagtttcg ggattcatac
     7861 tggacgcatg gagctcatgg gcttggtctt atcaaatggt gtgattgccc ttgcaggtgc
     7921 cctcattgct cagcaagaag gttatgccga tgtgtctcga gggatcgggg ttatcgttgt
     7981 ggggcttgca agtttgatta ttggagaagt tattttcaag agtttgagct tggcagagcg
     8041 tttggttact atcgttgtag gttctatcgc ttatcaattt ttagtgtggg cagttatcgc
     8101 acttggcttt aatacaagtt accttcgttt atacagtgcc ttgattttag cagtctgcct
     8161 catgattcca acatttaagc aaacaatctt gaaaggagcc aagttaagca aatgacagca
     8221 attgtagaat taaaaaatgc aaccaaaatc gttaaaaatg gctttgatga agaaaagatt
     8281 attttaaatg atgtttcctt agaaattttt gaacgggact ttatcacgat tttgggcgga
     8341 aatggtgctg gaaaatcaac cctctttaac actatagcag ggaccttatc actaactagt
     8401 ggaactatcc gtattttagg tgaagaactc actaagtttt cacccgagaa gcgtgccaag
     8461 tacctgtctc gtgtcttcca agatccaaag atggggacag ctccccgtat gacggtcgct
     8521 gaaaatcttt taatcgccaa gtttcgtggt gaaaagcgtg gattgttacc acgacgcttg
     8581 actagctata aggatgaatt tcaggcaacc attgaaaaag taggaaatgg tcttgagaaa
     8641 cacttgaata caccgattga gttcttatca ggtggacaaa gacaggcttt gagtctcttg
     8701 atggcaacct tgaagcgacc tgaattactc ctgttagatg agcatactgc tgccctggat
     8761 ccaaagacta gtgttgcttt gatggaattg acagatgaat ttgttaagaa agatcagcta
     8821 acagccctta tgattactca tcatatggaa gatgctctca aatacggcaa tcgcttgatt
     8881 gtcatgaaag aaggacggat tatccaagat ttaaaccaag aagaaaaagc aaaaatgaaa
     8941 atctctgatt attatcaact ctttgaataa agtagggaaa atgagtgaaa tggtttactt
     9001 tttttctgaa ataaagtata ctatataaag taaactatga taacatggag gtattgtgta
     9061 tggttgacaa acaagtcatt gaagaaatca aaaacaatgc caacattgtg gaagtcatag
     9121 gagatgtgat ttctttacaa aaggcaggac ggaactatct agggctctgt ccttttcatg
     9181 gtgaaaaaac accttctttc aacgttgtag aggataagca gttttatcac tgtttcggtt
     9241 gtggtcgctc aggtgatgtc tttaagttca tcgaggagta ccaaggggtt ccctttatag
     9301 aggcagtcca aatcttaggt cagcgtgttg gaattgaggt tgaaaaaccg ctttatagtg
     9361 agcagaagcc agcctcacct caccaagctc tttatgatat gcacgaagat gcagccaaat
     9421 tttaccatgc gattctcatg acaacgacta tgggcgaaga ggccagaaat tacctttatc
     9481 agcggggttt gacagatgaa gtgcttaaac atttttggat tggtttagca cctccagaac
     9541 gaaactatct ctatcaacgt ttgtctgatc agtatcgtga agaggattta ctggattcag
     9601 gcctgtttta tctctcggat gccaatcaat ttgtagacac ctttcacaat cgcattatgt
     9661 ttcccctgac aaatgaccaa ggaaaggtca ttgccttctc aggtcgtatc tggcaaaaaa
     9721 cggattcaca aacttctaag tataaaaaca gccgatcgac tgcaattttt aacaaaagtt
     9781 acgaattata tcatatggat agggcaaaaa aatcttctgg aaaagctagt gagatttact
     9841 tgatggaagg attcatggat gttattgcag cctatcgggc tggaatcgaa agtgctgtgg
     9901 cgtcgatggg aacggccttg agtcgagagc atgttgagca tctgaaaagg ttaaccaaga
     9961 aattggttct tgtttacgat ggagataagg ctgggcaagc cgcgacattg aaagcattgg
    10021 atgaaattgg tgatatgcct gtgcaaatcg tcagcatgcc tgataacttg gatcctgatg
    10081 agtatctaca aaaaaatggt ccagaagact tggcctatct attaacgaaa actcgtatta
    10141 gtccgattga gttctacatt catcagtaca aacctgaaaa cagtgaaaat ctgcaggctc
    10201 agattgagtt tcttgaaaaa atagctccct tgattgttca agaaaagtcc atcgctgctc
    10261 aaaacagcta tattcatatt ttagctgaca gtctggcgtc ctttgattat acccagattg
    10321 agcagattgt taatgagagt cgtcaggtgc aaaggcagaa tcgcatggaa ggaatttcca
    10381 gaccgacgcc aatcaccatg ccaatcacca agcagttatc ggctattatg agggcagaag
    10441 cccatctact ctatcggatg atggaatccc ctcttgtttt gaacgattac cgtttgcgag
    10501 aagactttgc atttgctaca cctgaatttc aggtcttata tgacttgctt ggccagtatg
    10561 gaaatcttcc tccagaagtt ttagcagagc agacagagga agttgaaaga gcttggtacc
    10621 aagttttagc tcaggatttg cctgctgaaa tatcgccgca ggaacttagt gaagtagaga
    10681 tgactcgaaa caaggctctc ttgaatcagg acaatatgag aatcaaaaag aaggtgcagg
    10741 aagctagcca tgtaggagat acagatacag ccctagaaga attggaacgt ttaatttccc
    10801 aaaagagaag aatggagtaa taatggcaac aaaacaaaaa gaagtaacaa catttgacgt
    10861 acaggtagca gaatttatcc gtaatcataa gcaaaaaggg acagcaacag atgatgaaat
    10921 caatgcaagt ctggttattc cttttacctt ggacgctgat gggattgaag atctcttgca
    10981 acggattcag gatgcaggaa tttctatcac agataacgaa ggaaatccaa gtgcgcgtgt
    11041 tcttagcaat gaagaagaac cagaactcag cgatgaggac ttgattgggt caacttctgc
    11101 taaggtcaat gaccctgtcc gtatgtactt gaaagaaata ggggtcgttc ctctcttgac
    11161 caatgaagag gagaaagagt tggcactggc tgttgaagct ggtgatatcg aagccaaaca
    11221 acgtcttgcg gaagccaatc ttcgtttggt tgtttccatt gccaaacgct atgtcggtcg
    11281 tggcatgcag ttccttgact tgattcaaga aggaaatatg ggcttgatga aggcggttga
    11341 caagtttgac tattctaaag ggttcaagtt ttcaacttat gcaacttggt ggattcgtca
    11401 ggctatcact cgtgctattg cggaccaagc tcgtaccatc cgtatcccag ttcacatggt
    11461 tgaaactatc aataaattgg ttcgtgaaca gcggaatctc cttcaagaat tggggcaaga
    11521 tccgacacca gaacagattg ctgaacgaat ggatatgaca cctgataagg ttcgtgaaat
    11581 cttgaagatt gcccaagaac cagtatctct tgaaactcct atcggtgaag aggacgatag
    11641 ccaccttgga gactttatcg aagatgaagt gattgaaaat ccagtggatt atacgactcg
    11701 tatcgtcttg cgtgagcaat tggatgaaat cttagatact cttacagacc gtgaagaaaa
    11761 tgttctgcgt ctacgttttg gactagatga tggaaaaatg cgcacacttg aagatgtggg
    11821 gaaagtcttt aacgtaactc gtgagcgtat ccgtcagatt gaagcaaagg ctttgagaaa
    11881 actacgccaa ccaagtcgta gcaaaccgct tcgtgatttt attgaagact aagagtgagg
    11941 aaaatatggc ttatacagaa gagcaaattg aaaacatcaa aacacggatt ttaacagcct
    12001 tggaagaagt catcgaccct gagttgggaa tcgatattgt caatcttggt ttgatctatg
    12061 agattcgttt tgacggtgac acagggcaaa cagagattga catgactttg acaactatgg
    12121 gttgtccctt agcagacctt ttgacagacc agatttatga tgctatgata gaggttccag
    12181 aagtaacgga tactgaggtt aaattagtct ggtatccagc ctggacagtt gaaaaaatga
    12241 gtcgctatgc ccgcattgcc ctaggcatta agtaaacata taaaagcatg tgagagactg
    12301 ttggaaaagc gaggaaattt cccctctttt cctctagtct ctcctttctt ttgctgattt
    12361 tattcaaaga aaatgatata atagtagtta tggagaaaaa gaaattacgc atcaatatgt
    12421 tgagttcaag tgagaaagta gcaggacagg gagtttcagg tgcttaccgt gaattagttc
    12481 gtcttcttca ccgtgctgcc aaggaccaat tgattgttac agaaaatctt ccaatcgagg
    12541 cagatgtgac tcactttcat acgattgatt ttccctatta tttatcaacc ttccaaaaga
    12601 aacgctcagg gagaaagatt ggctatgtgc atttcttgcc agctacactt gagggaagtt
    12661 tgaaaattcc atttttctta aagggaattg tgaaacgcta tgtattttct ttttacaacc
    12721 ggatggagca cttggttgtg gtcaatccta tgtttattga ggatttggta gcagctggta
    12781 ttccacgtga aaaagtgacc tatattccta actttgtcaa caaggaaaaa tggcatcctc
    12841 taccacaaga agaggtagtc agactgcgca cagatcttgg tcttagtgac aatcagttta
    12901 tcgtagtagg tgctgggcaa gttcagaaac gtaaagggat tgatgacttt atccgtctgg
    12961 ctgaggaatt gcctcagatt acctttatct gggctggtgg cttctctttt ggtggtatga
    13021 cagatggtta tgaacactat aagaaaatta tggaaaatcc ccctaaaaat ttgatttttc
    13081 caggcattgt atcgccagag cggatgcgcg aattgtatgc tctagcggat cttttcttgt
    13141 tgcctagtta caatgagctc tttcctatga ctattttaga agctgcgagt tgtgaggctc
    13201 ctattatgtt gcgtgattta gatctctata aggtaatttt ggagggaaat tatcgggcga
    13261 cagcgggtag agaagagatg aaagaggcta ttttggaata tcaagcaaat cctgctgcct
    13321 taaaagatct caaagaaaag gctaagaata tttccagaga gtattctgaa gagcatctgt
    13381 tacaaatctg gttggacttt tatgagaaac aagccgcttt agggagaaag taaaaagtga
    13441 ggtaatctat gcgaattggt ttatttacag atacctattt tcctcaggtt tctggtgttg
    13501 cgaccagtat tcgaaccttg aaaacagaac ttgaaaagca gggacatgct gtttttatct
    13561 ttacgacgac agataaggat gtcaatcgct acgaagattg gcaaattatc cgcattccaa
    13621 gtgttccttt ctttgctttt aaggatcgtc gctttgccta ccgaggtttt agcaaggcac
    13681 ttgaaattgc taaacagtat cagctagata ttatccatac tcagacagaa ttttctcttg
    13741 gcctgttggg gatttggatt gcgcgtgaat tgaaaattcc agtcatccat acctatcaca
    13801 cccagtatga agactatgtc cattatattg ctaaggggat gttgatccgg ccgagtatgg
    13861 tcaagtatct ggttagaggt ttcctgcatg atgtggatgg ggttatttgt cctagtgaga
    13921 ttgtccgtga cttgctatct gattataagg tcaaggttga aaaacgggtc attcctactg
    13981 ggattgaatt agccaagttt gagcgtccgg aaatcaagca ggaaaatttg aaagaactgc
    14041 gtagtaaact agggattcaa gatggtgaaa agacgttgct tagtctttcg agaatctcct
    14101 atgaaaaaaa tattcaagca gttttagcag cctttgctga tgttctgaaa gaggaagaca
    14161 aggttaaact ggtagtagct ggggatggcc cttatctgaa tgacctcaaa gagcaagctc
    14221 agaacctaga gattcaagac tcagtcatct ttacagggat gattgctcct agtgagacgg
    14281 ctctttacta taaagcggcg gatttcttca tttcggcatc gacaagcgaa acgcaaggtt
    14341 tgacctactt ggaaagctta gccagtggaa cacctgtcat tgctcacgga aatccttatt
    14401 tgaacaacct catcagtgat aaaatgtttg gaaccttgta ctatggagaa catgatttgg
    14461 ctggtgctat tttggaagcc ctgattgcaa caccagacat gaacgagcat accttatcag
    14521 agaaattgta tgagatttca gctgagaact ttgggaaacg agtgcatgag ttttatctgg
    14581 atgccattat ttcaaataac ttccagaaag atttggctaa agatgatacg gtcagtcagc
    14641 gtatctttaa gacagttttg tatcttcagc aacaggtggt tgctgtacct gtaaaaggat
    14701 ctagacgcat gttgaaggct tcaaaaacac agttgatcag tatgagagac tattggaaag
    14761 accatgaaga atagaaagag gaacagctat gaaaaaaaca attaatgaga agcggtcgtg
    14821 ataaaaagat tgcgggtgtt tgtgctgggg tggcccatta tctggatatg gatccgacta
    14881 tcgttcaagt catttggggt gttcttactt gctgttacgg agctggaatt gtagcttaca
    14941 ttattttatg gattatcgca ccagtagcaa ctgactattg aaaactagct caattcatgc
    15001 taagataata gaaaatagaa tgtaacgaag gagagaaaaa tggcatttgg agataatgga
    15061 aatcgtaaaa aaactatgtt tgagaaaata accttgttta tcgtgattat catgctagta
    15121 gcaagtttat tgggaatttt tgcaactgca attggtgccc tcagtaatct ataaaataga
    15181 ttcaagaaaa tttagtgact gggatttccc agcccttttt taaagtgaga agaaataatg
    15241 agtatgtttt tagatacagc taagattaag gtcaaggctg gtaatggtgg cgatggtatg
    15301 gttgcctttc gtcgtgaaaa atatgtccct aatggaggcc cttggggtgg tgatggtggt
    15361 cgtggaggca atgtggtctt cgttgtagac gaaggactac gtaccttgat ggatttccgc
    15421 tacaatcgtc atttcaaggc tgattctggt gaaaaaggga tgaccaaagg gatgcatggt
    15481 cgtggtgctg aggaccttag agttcgagta ccacaaggta cgactgttcg tgatgcggag
    15541 actggcaagg ttttaacaga tttgattgaa catgggcaag aatttatcgt tgcccacggt
    15601 ggtcgtggtg gacgtggaaa tattcgtttc gcgacaccaa aaaatcctgc accggaaatc
    15661 tctgaaaatg gagaaccagg tcaggaacgt gagttacaat tggaactaaa aatcttggca
    15721 gatgtcggtt tagtaggatt cccatctgta gggaagtcaa cacttttaag tgttattacc
    15781 tcagctaagc ctaaaattgg tgcctaccac tttaccacta ttgtaccaaa tttaggtatg
    15841 gttcgcaccc aatcaggtga atcctttgca gtagccgact tgccaggttt gattgaaggg
    15901 gctagtcaag gtgttggttt gggaactcag ttcctccgtc acatcgagcg tacacgtgtt
    15961 atccttcaca tcattgatat gtcagctagc gaaggccgtg atccatatga ggattaccta
    16021 gctatcaata aagagctgga gtcttacaat cttcgcctca tggagcgtcc acagattatt
    16081 gtagctaata agatggacat gcctgagagt caggaaaatc ttgaagaatt taagaaaaaa
    16141 ttgtctgaaa attatgatga atttgaagag ttaccagcta tcttcccaat ttctggattg
    16201 accaagcaag gtctggcaac acttttagat gctacagctg aattgttaga caagacacca
    16261 gaatttttgc tctacgacga gtccgatatg gaagaagaag cttactatgg atttgacgaa
    16321 gaagaaaaag cctttgaaat tagtcgtgat gacgatgcga catgggtact ttctggtgaa
    16381 aaactcatga aactctttaa tatgaccaac tttgatcgtg atgaatctgt catgaaattt
    16441 gcccgtcagc ttcgtggtat gggggttgat gaagcccttc gtgcgcgtgg agctaaagat
    16501 ggggatttgg tccgcattgg taaatttgag tttgaatttg tagactagga gactggtatg
    16561 ggagataaac cgatatcttt ccgagatgcg gatggtaatt ttgtttccgc cgcagacgtt
    16621 tggaatgaaa agaaattgga agaactattt aatcgtctca atccaaatcg tgccttgaga
    16681 ttggcacgaa ctaaaaagga aaatccatct cagtaaagaa gctaaaaaat cccgtgcctc
    16741 atcagacacg ggattttgtg gtacgacagg catgtatagc aaactgaatc tggaatagca
    16801 cagcatatct tctaaaatat tgctataaat tgatttgaat ttcaaaatta aattgtttta
    16861 ttcttatttc aatttgttat agtatatctg atgtcaaagt tctcggcgag tcaaatagtg
    16921 attcccaagc ctgactatcg tgaggtagcg gattaaaatg gtctggggat agaccgtttt
    16981 aagtctgatg ctggaaataa gaattgtcag aagaagggat agcgaaatcg tggctctacg
    17041 aacaggaacg tgataataag gcgtatatag cgaataagag ggcctcaaac tctaaagtcc
    17101 aaaaaggtag tcgtaaccta tatgcgtaaa tcatgagagt aattgaattc gtactaagat
    17161 tttctatttt tactgtaacc ttttaacgtc ctcatatctt gtatacacga ggaaagatgt
    17221 acgacttatc ccgtgaggtc tatcactata aagagaaaac gacagataga agtgatcctg
    17281 agtcacggtt atctttaaaa tagattgtct gataggatgc tagcctgaaa acgtcttgtt
    17341 catgaaatat aggacggtat gtataaaacg cttctgtgaa ctgagagaag ggggagaagt
    17401 tcttgctaaa atttagttga acagccgtat tccgatactt agataagaga tctagtctta
    17461 gctcctactc agtttcaggg gataaaaaag gggcaatagc gattcgagaa agattatact
    17521 cttcgaaaat ctcttcaaat cacgtcaata tcgccttgcc gtatgtgtag gatactaact
    17581 acgtcagttc catctacaat ctcaaaacag tgttttgagc aacctgcggc tagtttccta
    17641 gtttgatctt tgatttttat tgagtattag taattcagtt actaactcgt caactctgat
    17701 ttatccaata aaattgaaaa ggatggaaaa aaggataaat ttatgatata ctttattttg
    17761 aagaccttat tagaaatctt gaaagagtat tgaaaactta gaatgagaaa aattgttatc
    17821 aatggtggat taccactgca aggtgaaatt actattagtg gtgctaaaaa tagtgttgtg
    17881 gccttaattc cagctattat attggctgat gatgtggtga ctttggattg tgttccagat
    17941 atttcggatg tagccagtct tgtcgaaatc atggaattaa tgggagctac tgttaagcgt
    18001 tatgacgatg tcttggagat tgatccaaga ggtgttcaaa atattccaat gccttatggt
    18061 aaaattaaca gtcttcgtgc atcttactat ttttatggga gcctcttagg ccgttttggt
    18121 gaagcgacag ttggtctacc gggaggatgt gatcttggtc ctcgtccgat tgacttacac
    18181 cttaaggcgt ttgaagctat gggtgccact gctagctacg agggagataa catgaagtta
    18241 tctgctaaag atacaggact tcatggtgca agtatttaca tggatacggt tagtgtggga
    18301 gcaacgatta atacgatgat tgctgcggtt aaagcaaatg gtcgtactat tattgaaaat
    18361 gcagcccgtg aacctgagat tattgatgta gctactctct tgaataatat gggtgcccat
    18421 atccgtgggg caggaactaa tatcatcatt attgatggtg ttgaaagatt acatgggaca
    18481 cgtcatcagg tgattccaga ccgcattgaa gctggaacat atatatcttt agctgctgca
    18541 gttggtaaag gaattcgtat aaataatgtt ctttacgaac acctggaagg gtttattgct
    18601 aagttggaag aaatgggagt gagaatgact gtatctgaag acagcatttt tgtcgaggaa
    18661 cagtctaatt tgaaagcaat caatattaag acagctcctt acccaggctt tgcaactgat
    18721 ttgcaacaac cgcttacccc tcttttacta agagcgaatg gtcgtggtac aattgtcgat
    18781 acgatttacg aaaaacgtgt aaatcatgtt tttgaactag caaagatgga tgcggatatt
    18841 tcgacaacaa atggtcatat tttgtacacg ggtggacgtg atttacgtgg ggccagtgtt
    18901 aaagcgaccg acttaagagc tggggctgca ctagtcattg ctgggcttat ggctgaaggt
    18961 aaaactgaaa ttaccaatat cgagtttatc ttacgtggtt attctgatat tatcgaaaaa
    19021 ttacgtaatt taggagcgga tattagactt gttgaggatt aaaccgtaga ggtgtttatg
    19081 aatatttgga ccaaattagc aatgttttct ttttttgaaa cggatcgctt gtatttgcgt
    19141 cctttctttt ttagtgatag tcaggacttc cgcgagatag cttcaaatcc agaaaatctt
    19201 caatttattt tcccaacgca ggcaagtctg gaagaaagtc aatatgcact ggccaattac
    19261 tttatgaagt cccctttggg agtgtgggca atttgtgacc agaaaaatca acaaatgatt
    19321 ggttctatta aatttgagaa gttagatgaa atcaaaaaag aagctgagct tggctatttt
    19381 ttgagaaaag atgcttggtc gcaaggattt atgacagagg ttgttagaaa aatttgtcag
    19441 ctttcttttg aggaatttgg cttaaaacaa ttatttatca ttacccacct tgaaaatgaa
    19501 gctagccaaa gagttgctct taagtctgga tttagtttgt tccgtcagtt taagggaagt
    19561 gatcgttaca caagaaaaat gcgggattat cttgaatttc ggtatgtaaa aggagagttc
    19621 aatgagtaag catcaggaaa ttctaagcta tttggaggaa ttaccagtag gtaaaagggt
    19681 cagtgttcgt agcatttcga atcatctagg agttagtgat ggaacagcct atcgagctat
    19741 taaagaagct gaaaaccgtg gaattgtgga gacccgtcct agaagtggaa caattcgtgt
    19801 taaatcccag aaagttgcta tagagagatt aacgtttgct gaaattgcag aagtgacttc
    19861 ttctgaggtt ctggctgggc aagaaggttt agagagagag tttagtaagt tttcaattgg
    19921 tgccatgact gaacaaaata tcttgtctta ccttcatgat ggggggctct tgattgtcgg
    19981 agaccgaacc cgtattcagt tgctagcctt ggaaaatgaa aatgcagttc tggttacagg
    20041 gggatttcag gttcatgatg atgtgcttaa actggccaat caaaaaggga ttcctgttct
    20101 aagaagtaag catgatacct ttaccgtcgc gaccatgatc aataaagcct tgtcaaatgt
    20161 ccaaatcaag actgatattc tgacagttga gaaactttat cgccctagtc atgagtatgg
    20221 ttttctgaga gagacagata cagttaaaga ttatttggac ttggttcgta agaatcgtag
    20281 cagccgtttc cctgttatca atcaacatca ggtcgttgtt ggtgttgtaa ccatgagaga
    20341 cgctggtgat aaatcaccaa gcacgacaat tgataaggtt atgtctcgta gtctattttg
    20401 ggttggatta tcgacaaata ttgccaatgt gagtcaacgg atgatcgcag aagactttga
    20461 aatggtacca gttgttcgaa gcaatcaaac tttgcttggc gttgtgacgc gacgagatgt
    20521 catggagaag atgagccgtt cccaagtttc ggctctacca actttttctg agcagattgg
    20581 acaaaagctc tcttatcacc atgatgaagt agtcattaca gtggaaccct ttatgctaga
    20641 aaaaaatgga gttttggcta atggtgtatt ggcagaaatt ctgacccaca tgacccaaga
    20701 tttagttgtt aatagtggtc gcaatctcat tatcgagcag atgctgatct actttttgca
    20761 ggctgttcag atagatgata tattgcgcat tcaggcacgg attattcatc atacgagacg
    20821 gtcagctata attgattacg atatttatca tggtcaccag attgtttcaa aagcaaatgt
    20881 gactgttaaa attaattaga aactaggaga aaagatgata acattaaaat cagctcgtga
    20941 aatcgaagct atggacaagg ctggtgattt tctagcaagt attcatatag gcttacgtga
    21001 tttgattaag ccaggcgtag atatgtggga agttgaagaa tatgtccgcc gtcgttgtaa
    21061 agaagaaaat ttccttccac ttcagattgg ggttgacggt gccatgatgg actatcctta
    21121 tgctacctgt tgctctctta acgatgaagt ggctcacgct ttccctcgtc attatatctt
    21181 gaaagatggt gatttgctca aagttgatat ggttttggga ggtcccattg ctaaatccga
    21241 cctgaatgtc tcaaaattaa acttcaacaa tgttgaacaa atgaaaaaat acactcagag
    21301 ctattctggt ggtttagcag actcatgttg ggcttatgct gttggtacac cgtccgaaga
    21361 agtcaaaaac ttgatggatg taaccaaaga agctatgtac aagggtattg agcaagctgt
    21421 tgttggaaat cgtatcggtg atatcggtgc ggctattcaa gaatacgctg aaagtcgtgg
    21481 ttacggtgta gtgcgtgatt tggttggtca tggtgttggc ccaactatgc acgaagaacc
    21541 aatggttcct aactatggta ttgcaggtcg tggactccgt cttcgtgaag gaatggtctt
    21601 aaccattgaa ccaatgatca atacaggcga ttgggaaatt gatacagata tgaaaactgg
    21661 ttgggcgcat aagaccattg acggtggatt gtcatgtcag tatgaacacc aatttgtcat
    21721 tacgaaagat ggacctgtta tcttgactag ccaaggtgaa gaaggaactt attaataaaa
    21781 agtgaaaaga ctactggaag tttattttga taaaaaatcc agtagatctt ttcataataa
    21841 aacgcattgt atcaagtgtt aggagctgat atcatgcgtt tttctgcttt taagattttt
    21901 tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc tctcctccta
    21961 tatactatta gtaaagtaaa actaagagag gatattttag tgtcgcagtc tattgttcct
    22021 gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat tctgtttaac
    22081 gttcgtattg ccaatcttaa atttaccttt tttagattag acttcctgcg aaataaaata
    22141 tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc acgatttaag
    22201 cgtcttgttg gtgttcagcg cacgactttt gaagagatgt tagctgtatt aaaaacagct
    22261 tatcaactta aacacgcaaa aggtggacga aaacctaaat taagcctaga agaccttctt
    22321 atggccactc ttcaatatgt gcgagaatat cgaacttatg aagaaattgc ggctgttttt
    22381 ggtatccacg aaagcaactt aatccgtcgg agctaatggg ttgaagtaac tcttgttcaa
    22441 agtggtgtta cgatttcaag aactcctctc agctctgagg ccacggtaat gattgatgcg
    22501 acggaagtaa aaatcaatcg ccctaaaaaa acaattagcg aattattctt gtaaaaaaat
    22561 gccacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt tctttggata
    22621 tcactgtgaa ctattgtcat gatatgaagt tgttcaaaat gagttgcaga aatatcggac
    22681 aagctggtaa aatcttggct gacagtggtt atcaagggct catgaagata tatcctcaag
    22741 cacaaactcc acgtaaatcc agcaaactca agccgctaac agttgaagat aaagcctgta
    22801 accatgcgct atccaaggag agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt
    22861 ttaaaataat ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt
    22921 tgagtgctgg tattatcaat catgaactag gattctagtt ttgcaggaag tctattgagg
    22981 tattgagcta gtttatgaaa aaattgggtg aaaagtcgag tgttttagaa acccacagtg
    23041 tagtattcta gtttcaatcc actatatttt gctactcccc gtaaagtttc tattttccct
    23101 gatttctgat ataatagaaa tattgacttc aagagtaagg aagagaagat gaacgcatta
    23161 ttaaatggaa tgaatgaccg tcaggctgag gcggtgcaaa cgacagaagg tcccttgcta
    23221 atcatggcag gggctggttc tggaaagact cgtgttttaa cccaccgtat cgcttatttg
    23281 attgatgaaa agctggtcaa tccttggaat atcttggcca ttacctttac caacaaggct
    23341 gcgcgtgaga tgaaagagcg tgcttatagc ctcaatccag ctactcagga ctgtctgatt
    23401 gcgaccttcc actccatgtg tgtgcgtatt ttgcgtcgcg atgcggacca tattggctac
    23461 aatcgtaatt ttacaattgt ggatcctggt gaacagcgaa cgctcatgaa acgtattctc
    23521 aaacagttga acttggatcc taaaaaatgg aatgaacgaa ctattttggg gaccatttcc
    23581 aatgctaaga ataatttgat tgatgatgtt gcttatgctg cccaagctgg cgatatgtat
    23641 acgcaaattg tggcccagtg ttatacagcc tatcaaaaag aacttcgtca gtctgaatcc
    23701 gttgactttg atgatttgat tatgctgacc ttgcgtctct ttgatcaaaa tcctgatgtt
    23761 ttgacctact accagcaaaa attccaatac atccacgttg atgagtacca agataccaac
    23821 cacgctcagt accaattggt caaactcttg gcttcccgtt ttaaaaatat ctgtgtggtt
    23881 ggggatgcgg accagtctat ctacggttgg cgtggtgctg atatgcagaa tatcttggac
    23941 tttgaaaagg attaccccaa agccaaggtt gtcttgctgg aggaaaatta ccgctcaacc
    24001 aaaaccattc tccaagcggc caacgaggtt attaaaaata ataaaaatcg ccgtcctaaa
    24061 aatctctgga ctcaaaacgc tgatggggag caaatcgttt actatcgtgc cgatgatgag
    24121 ctggatgagg ctgtatttgt agccagaacc atcgatgaac ttagtcgcag tcaaaacttc
    24181 cttcataagg attttgcagt tctctatcgg actaatgccc agtcccgtac aattgaggaa
    24241 gccctgctca agtctaacat tccttatacc atggttggcg gaaccaaatt ctacagccgt
    24301 aaggaaattc gcgatattat tgcttatctc aaccttattg ctaatttgag tgacaatatt
    24361 agttttgagc gtattatcaa cgagcctaaa cgtggaattg gtctaggtac agttgagaaa
    24421 atccgtgatt ttgcaaattt gcaaaatatg tctatgctgg atgcttctgc taatattatg
    24481 ttgtctggta tcaagggtaa ggcagcccaa tctatctggg attttgccaa tatgatgctt
    24541 gatttgcggg agcagctaga ccacttaagc attacagagt tggttgagtc cgtcctagaa
    24601 aaaacaggtt atgtcgatat tcttaacgcc caagcgactc tagaaagcaa ggcacgggtt
    24661 gaaaatatcg aagagtttct ttctgttacg aagaactttg atgacaccac ggatgtgaca
    24721 gaagaggaaa ctggtctgga caaactgagt cgtttcttaa atgacttggc tttgattgcc
    24781 gacacagatt caggtagtca ggagacatca gaagtgacct tgatgaccct gcatgctgcc
    24841 aaaggtctcg aatttccagt tgtctttttg attgggatgg aagaaaatgt ctttccactt
    24901 agtcgtgcga ctgaagatcc agatgaatta gaagaagagc gccgtctagc ctatgtaggt
    24961 atcacgcgtg cagagaaaat tctctatctg accaatgcca actcacgctt gctttttggt
    25021 cgtaccaatt ataaccgtcc gactcgtttt attaacgaaa tcagttcaga cttgcttgag
    25081 tatcaaggtc tggctcgtcc tgcaaataca agctttaagg catcatatag cagtggtagt
    25141 atttcctttg gtcaaggtat gagtttggct caggctcttc aagaccgtaa acgcggtgct
    25201 gccccaaaat caatccagtc aagcggtctt ccatttggtc aatttacagc tggcgcaaaa
    25261 ccagcatcta gcgaggcaaa ttggtccatt ggtgatattg ctctccacaa gaaatgggga
    25321 gagggaaccg ttctggaagt ttcaggtagc ggtgctaggc aggaattgaa aatcaatttc
    25381 ccagaagtag gtttgaaaaa acttttagcc agtgtggctc caattgagaa aaaaatctaa
    25441 ttttccatcc ttctcacgaa taataaagtg aggaggattt ttatgtacag tatttcattc
    25501 caagaagatt cactattacc aagagaaagg ctggccaagg aaggagttga agcgcttagt
    25561 aaccaagagt tgctagctat tttactcagg acaggaacac gtcaagttag cgtttttgaa
    25621 attgcccaaa aagtcttgaa caatctttca agcctaacgg atttgaaaaa aatgaccctg
    25681 caggaattgc agagtttgtc tggtattggg cgtgttaagg ccatagaatt acaagctatg
    25741 attgaactgg ggcatcgtat tcacaaacac gagactcttg aaatggaaag tattctcagc
    25801 agtcaaaagt tggccaagaa gatgcagcag gaattagggg ataaaaaaca agagcacctg
    25861 gtggcactct atctcaatac tcaaaatcaa atcatccatc agcagaccat ttttatcggg
    25921 tctgtaactc gtagtatcgc tgaaccgcga gagattcttc actatgcaat caagcatatg
    25981 gcgacttctc ttatcttggt ccacaatcat ccttcaggag cggtagcgcc tagccaaaat
    26041 gatgatcatg tcactaaact tgttaaagaa gcctgcgaat tgatggggat tgttctcttg
    26101 gaccatttga ttgtctctca ttctaattac tttagttatc gtgaaaagac agatttaatc
    26161 taaagttcat taacgacata gtcaaagagt tttttatctt tgggacgatt ttcaaaaaga
    26221 agttctggat gccattggac accgagaaag gcgacatcat ccgtactcat gacagcctca
    26281 atgataccat ctttaggatc atgagccaca acttttaaat ttggtgctaa gtccttgatg
    26341 ctctggtggt ggaaggagtt gatatgagag atttctccat agatttcttg gagaacggta
    26401 tctggttctg ttaccaagcg ttgagttgtg tactcaacag aagaatcctg ccaatggtct
    26461 tcgatatctt ggtacaaagt tccacccatg gcaacgttaa agagttgggt accacggcag
    26521 acagagaaaa tgggcttttt ctgtttaata gcttccttga tgagggtcag ttcgaagata
    26581 tctctttgaa ggtgatagtc atcgctatca atggttttgg gttcgccata aaattttgga
    26641 tcgacatttt gcccacctgt caagatgagc ttgtcaatca aactgatata gtggcaggcc
    26701 atttcttgat caccaatcgg taggatgatg ggaatccctc cagcatcttt aacgccttca
    26761 acaaagcctt ttgctgcgta gctcatcatg atgtcatcat ctggatgagc tttttcgttt
    26821 cctgtaatcc caataactgg ttttttcata aaatgatttt cgctttctaa tcctcttttc
    26881 gcatgaagta gaggagggtt tggagttcac ttgtcaaatc gacatactga acgaccacgt
    26941 cttttggtaa atgcagatgg actggtgaaa aactgagaat tcctttcaca ccagcatcaa
    27001 ccaagagatt agcaacctct tgtgacttga cgctgggaac agttaggata gcagtcttca
    27061 catcagcatc cttgatttta tccttgatct gagaaatccc gtaaatggga atcccgtcag
    27121 gagtttgggt accgacttca ggatggtcgt ctaggtcaaa ggccatgata atcttcatct
    27181 tgttacgttc gtggaagcgg tagtggagaa gggcatggcc catatttcca ataccaacca
    27241 gcatgacatt ggtaatagag ttgtcattga gcaaatcggc aaaaaatgtc attagttttt
    27301 tgacatcata gccaaaacca cgacgaccaa gttcaccaaa ataggaaaaa tcacgacgta
    27361 cggtcgctga atcaataccg atagcctctg caatttgctt agagttggca cgttcaatct
    27421 tttctgcatg aaatctctta aaaattcgat agtagagaga gagtcttttt gctgtagctt
    27481 ttggaatagc aaactgttta tctttcacaa aatcacaacc tttctattct tctattttat
    27541 agaaacattg tgaaaaaatc aacaaaaata agaaaaaact aagaaaaatc ttagttttga
    27601 tgtaaaaaat ctgcatgaga tagaaaacgg tagaggtctc cgaccagacc ctgataaact
    27661 ttttggcccc taaaagtcag agaagtcaca taaagtgtat ctggtaaggt tacacatcct
    27721 gacaaagtca acatgagagc ctcatgatcc tcatacttga gagtacgctc tacatgatag
    27781 cagtccttat aggtcagttc aaacattttg gctctatctt tccgattttg taaagacacc
    27841 acgttctacc aagctatcca tgaggaagta gaatttttcc tgatgaatat ggtggtcttc
    27901 tgatttgaaa atatcaacta gacgaaggcc aaacttgtca gtgatattga ttttagcccc
    27961 tgtaagttcc ttgttaatga tgattttgag ttggaagcct tcaccgctgt ttggcacttt
    28021 ttccaaaagg cgagtcagtt catagttacc aaccttagtt tcaaaaaagg tgttatcttt
    28081 gagggtgaat tttttaacag aagggctaag agtgtaatcg taacgacaat tttttaactg
    28141 aatgattttt tcaaatgcca tatggctaac ctccgataat ttcttttaag gtttttgcga
    28201 gggtttgtag gtcttcaacg gtattttgtg gcgacaaact gatgcgaagg gattccttca
    28261 agcgttctga atttgcgcca tacatggctt caagaacatg gctggattgg acaacgcctg
    28321 cagtacaggc tgagccagta gagattgaaa ttccagctaa atctagccga aggagtaaga
    28381 ggtcattttt ctgaccagga aatccaatat tgagaacata agggagatga tgttttcctc
    28441 tattcaagta atactgaatg ccctccagct ctgccagaaa ggcagtttct agattttgta
    28501 catgttgaaa atgttcttct tgtttttcta ggtcttcttt tagggctgca accatgccta
    28561 cgatagcagg cagattttca gttcctgcac gttttttctg ttcctggtct ccgccatgta
    28621 gataggaatc aaagtccatg ctagatgcgt agagaaaacc gattccctta ggaccatgga
    28681 atttgtgggc agaagcagtg agaaaatcaa tgcccaattc ttctgaatga attgggattt
    28741 taccaatagc ctgaactgca tcaacatgat aggcagcagg gtgttgcttg agtatttggc
    28801 caatttcagc gatgggcagt aggtttcctg tctcattatt ggcaaacatg gtagaaacca
    28861 aaatcgtatc gtcacgtaaa gccttttgaa tttgctgggc tgtgatttct tgattttctg
    28921 gctggataat ggttgcttca aacccaaagt gttgaaccaa gtaatcaatt gtttcaagga
    28981 cagcatggtg ctcgatggca gttgtgatga tatgttttcc ttgttcttgg tgacgaagac
    29041 agtagccaat gatggtagta ttattgcctt cagtcccacc agaagtgaaa aagatatgtt
    29101 gaggttttgt ccttagtaac tgggctagtt cctgacgggc ttctcgcaag agtttgccag
    29161 cttgacgacc atgaccatga atactagaag gatttccgtg ggtttcttgc ataaccttgg
    29221 tcatagctga aatagcaact gctgacatag gagtcgttgc agcattgtcc aaataaatca
    29281 aagaatcacc ttatttcttt ttattgtagg caaagagtgg gctgactggt tttctttcgt
    29341 gaatacggac gatagcatca ccaattaact cactagcagt gatgtagcat acatttttag
    29401 gagttttttc ttttgttgct actgaatcag tcacaagaat ttctttaata ttagtattgt
    29461 caagaagctc agcagctccc tcgacgaaga gaccgtggct agaaacagca taaatttctg
    29521 tagctccttc acgttcaacg attttagaag cttcagagaa ggtacgtcct gtatttaaaa
    29581 tatcatcaat caagatagct ttcttacctt caacatcacc aataatataa ccttcgttac
    29641 gagttgcatc gtcttgaggg tagtcgataa tggcgatagg agcatcaaga tattcagcca
    29701 ggctacgcgc acgtttgaca cctgaatttt tagggctaac gacaacaaca tctgaaccaa
    29761 gcaatccttt atcgcagtaa tgttttgcga ataggggaac agtgaaaaga ttatccactg
    29821 gaatatcaaa gaaaccttga acctgaacgg catgcaaatc aagagtcagg atacgatcaa
    29881 ctccagcctt aaccagcata ttggcaacta gttttgctgt aagtggctca cgaggacaag
    29941 caatgcggtc ttgacgtgca tagccaaaat atggaaggac aacgttgata ctgtgggcac
    30001 ttgcacgcac acaagcatcg accatgatta acaattccat taggtggttg ttgacaggga
    30061 aacttgttga ttggatgatg taaacatcat aaccacggac actttcttcg atatttactt
    30121 ggatttctcc gtctgaaaat tgacgtgatg atagttttcc aagtgggaca ccaacagctt
    30181 gggcaatttt ttgtgcaatc tcttggttag agttgagtgc gaaaagtttc atgttttttc
    30241 tatctgacat tatagaccgt cctctgtaaa ctttataaat cctagttata tttaccttac
    30301 atatatgaac tgggatttgt gtatttttat cttttctatt ttaccaaaaa atggagatta
    30361 tttcagctat ttttcatact tttgacaaat cgaaccaatt ttgaaggagc tttttggtag
    30421 gaaatctgat ttttctctaa aaattgtcgg aaatcctgtt tgccttgctc atgattttcc
    30481 acttcaagct ccaattcgta atctgttata tcaaagtatc ggctctgatc cagtgccatg
    30541 agaccaatag ctgttttcat ttcatagcga agcgttgtta gacaaccaag aacctgccag
    30601 ttcttacttt ggataccatg tttcgccaat tcatccagta ctagcccttg aggaagttct
    30661 tccttactca gatagttctc agcatctttt agttgcaatt tttggttgta ttccatgttt
    30721 ccaacactct gcgggacttt gagtgtcaac tcagcccagt cttcaaaggt tcgaatgcgc
    30781 atagcgactt tcttttctcg cagttcaaaa tcaggcgtgt cgatgtagta atttgtttga
    30841 agaacaggag tgacacctgt gaactggtct tttagacgat tgtattcatc ttttttcaat
    30901 agtgttttca attcaatttc taaatgtttc atttttctta ccttttttta tcgttgaaag
    30961 cggatttatg gtataataag cattgtattt attgtatatg aatctggaga aaaaatcaaa
    31021 gatatttttg acggataata tgagaacaag ggagaatata tgaccttaga atgggaagaa
    31081 tttctagatc cttacattca agctgttggt gagttaaaga ttaaacttcg tggtattcgt
    31141 aagcaatatc gtaagcaaaa taagcattct ccaattgagt ttgtgaccgg tcgagttaag
    31201 ccaattgaga gcatcaaaga aaaaatggct cgtcgtggca ttacttatgc gaccttggaa
    31261 cacgatttgc aggatattgc tggcttacgt gtgatggttc agtttgtaga tgacgtcaag
    31321 gaagtagtgg atattttgca caagcgtcag gatatgcgaa tcatacagga gcgagattac
    31381 attactcata gaaaagcatc aggctatcgt tcctatcatg tggtagtaga atatacggtt
    31441 gataccatca atggagttaa gactattttg gcagaaattc aaattcgtac tttggccatg
    31501 aatttctggg caacgataga acattctctc aactacaagt accaagggga tttcccagat
    31561 gagattaaga agcgactgga aattacagct agaatcgccc atcagttgga tgaagaaatg
    31621 ggtgaaattc gtgatgatat ccaagaagct caggcacttt ttgatccttt gagtagaaaa
    31681 ttaaatgacg gtgtaggaaa cagtgacgat acagatgaag aatacaggta aacgaattga
    31741 tctgatagcc aatagaaaac cgcagagtca aagggttttg tatgaattgc gagatcgttt
    31801 gaagagaaat cagtttatac tcaatgatac caatccggat attgtcattt ccattggcgg
    31861 ggatggtatg ctcttgtcgg cctttcataa gtacgaaaat cagcttgaga aggtccgctt
    31921 tatcggtctt cacactggac atttgggctt ctatacagat tatcgtgatt ttgagttgga
    31981 caagctagtg actaatttgc agctagatac tggggcaagg gtttcttacc ctgttctgaa
    32041 tgtgaaggtc tttcttgaaa atggtgaagt taagattttc agagcactca acgaagccag
    32101 catccgcagg tctgatcgaa ccatggtggc agatattgta ataaatggtg ttccctttga
    32161 acgttttcgt ggagacgggc taacagtttc gacaccgact ggtagtactg cctataacaa
    32221 gtctcttggc ggtgctgttt tacaccctac cattgaagct ttgcaattaa cggaaattgc
    32281 cagccttaat aatcgtgtct atcgaacact gggctcttcc attattgtgc ctaagaagga
    32341 taagattgaa cttattccaa caagaaacga ttatcatact atttcggttg acaatagcgt
    32401 ttattctttc cgtaatattg agcgtattga gtatcaaatc gcccatcata agattcactt
    32461 tgtcgcgact cctagccata ccagtttctg gaaccgtgtt aaggatgcct ttatcggtga
    32521 ggtggatgaa tgaggtttga atttatcgca gatgaacatg tcaaggttaa gaccttctta
    32581 aaaaagcacg aggtttctaa gggattgctg gccaagatta agtttcgagg tggagctatt
    32641 ctggtcaata atcaaccgca aaatgcaacg tatctattgg acgttggaga ctacgttacc
    32701 attgacattc ccgctgagaa aggctttgaa accttggagg ctattgagct tccattagat
    32761 attctctatg aggatgacca ctttctagtc ttgaataaac cctatggagt ggcttctatt
    32821 cctagtgtta atcactctaa taccattgcc aattttatca agggttacta tgtcaagcaa
    32881 aattatgaaa atcagcaggt tcacattgtt accagactag atagggacac ttctggcttg
    32941 atgctctttg ccaagcacgg ttatgcccat gcacgattag acaagcagtt gcagaagaaa
    33001 tctatcgaga aacgctactt tgctttggtt aagggagatg gacatttgga accagaaggg
    33061 gaaattattg ctccgattgc gcgtgatgaa gattccatta ttaccagacg agtggctaaa
    33121 ggcggaaagt atgcccatac ttcatacaag attgtagctt cttatggaaa tattcacttg
    33181 gtctatattc acctgcacac tggtcgaacc catcaaatcc gagtccattt ttctcatatc
    33241 ggttttcctt tgctgggaga tgatttgtat ggtggtagtc tggaagatgg tattcaacgt
    33301 caggctctgc attgccatta cctatccttt tatcatccat ttttagagca agacttgcag
    33361 ttagaaagtc ccttgccgga tgatttcagt aaccttatta cccagttatc aactaatact
    33421 ctataaaaac tgtctcagag tataattatt atcttaaagg agaaaactca tggaagtttt
    33481 tgaaagtctc aaagccaacc ttgttggtaa aaatgctcgt atcgttctcc ctgaagggga
    33541 agagcctcgt attcttcaag caacaaaacg cttagtaaaa gaaacagaag tgattcctgt
    33601 tttgcttgga aatcctgaaa aaattaaaat ttatcttgaa attgaaggaa tcatggatgg
    33661 ttatgaggtc atcgaccctc aacattatcc tcaatttgaa gaaatggttt ctgccttggt
    33721 ggagcgtcgc aagggcaaaa tgactgaaga agatgtacgc aaggttttgg ttgaagatgt
    33781 caactacttt ggtgtgatgt tggtttactt gggcttggtt gatggaatgg tgtcaggagc
    33841 gattcactca acagcttcaa cagttcgccc agctctacaa atcatcaaaa ctcgtccaaa
    33901 tgtaactcgt acttcaggag ccttcctcat ggttcgtggt acggaacgtt acctatttgg
    33961 agactgtgcc attaatatca atccagatgc agaagccttg gctgaaattg ccatcaactc
    34021 agcaatcaca gctaagatgt ttggcatcga acctaaaatt gccatgttga gctattctac
    34081 taaaggttca gggtttggtg aaagcgttga taaggtcgtt gaagcaacta aaattgctca
    34141 cgacttgcgt cctgaccttg aaatcgatgg tgagttgcaa tttgatgcgg cctttgttcc
    34201 cgaaactgca gctctgaaag ctccgggaag tacagtagct ggtcaagcaa atgtcttcat
    34261 cttcccaggt atcgaggcag gaaatatcgg ttacaagatg gctgaacgcc tgggtggctt
    34321 tgcggctgta ggacctgttt tgcaaggttt aaacaagcca gttaatgatc tttctcgtgg
    34381 atgtaatgca gatgatgttt acaagttgac cctcatcaca gcagctcaag cagttcatca
    34441 atagtgaaaa ctataaagtg atatactatg ctatactgta gttatgaaac tatgtacgaa
    34501 aagcactgcc attaattcct gagaactaaa ttactgattg gtgtcaaaaa ggaaaacttc
    34561 caagcgatga tatcctgtct atacacgacc tatagaaatc tgtaatatac atatccgtaa
    34621 aacgataaat tccctttttg attttaaatg agtatgaaaa gagaattttt tggctctttg
    34681 tcaactgtag tgggttgaag t
//
LOCUS       DAVKFH010000018        34531 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKFH010000018 DAVKFH010000000
VERSION     DAVKFH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.018"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            35..886
                     /locus_tag="VW006_000929"
     CDS             35..886
                     /locus_tag="VW006_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9379804.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(1118..1468)
                     /locus_tag="VW006_000930"
     CDS             complement(1118..1468)
                     /locus_tag="VW006_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379805.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(1608..1943)
                     /locus_tag="VW006_000931"
     CDS             complement(1608..1943)
                     /locus_tag="VW006_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379806.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(2031..3341)
                     /gene="der"
                     /locus_tag="VW006_000932"
     CDS             complement(2031..3341)
                     /gene="der"
                     /locus_tag="VW006_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9379807.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(3355..4068)
                     /locus_tag="VW006_000933"
     CDS             complement(3355..4068)
                     /locus_tag="VW006_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9379808.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(4065..4961)
                     /gene="dnaI"
                     /locus_tag="VW006_000934"
     CDS             complement(4065..4961)
                     /gene="dnaI"
                     /locus_tag="VW006_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9379809.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(4962..6131)
                     /locus_tag="VW006_000935"
     CDS             complement(4962..6131)
                     /locus_tag="VW006_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9379810.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(6132..6605)
                     /gene="nrdR"
                     /locus_tag="VW006_000936"
     CDS             complement(6132..6605)
                     /gene="nrdR"
                     /locus_tag="VW006_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9379811.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            6745..7110
                     /locus_tag="VW006_000937"
     CDS             6745..7110
                     /locus_tag="VW006_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9379812.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            7115..7810
                     /locus_tag="VW006_000938"
     CDS             7115..7810
                     /locus_tag="VW006_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379813.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            7827..8594
                     /locus_tag="VW006_000939"
     CDS             7827..8594
                     /locus_tag="VW006_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379814.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(8947..10146)
                     /locus_tag="VW006_000940"
     CDS             complement(8947..10146)
                     /locus_tag="VW006_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379815.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(10143..11036)
                     /locus_tag="VW006_000941"
     CDS             complement(10143..11036)
                     /locus_tag="VW006_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379816.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11153..11233
                     /locus_tag="VW006_000942"
                     /pseudo
     CDS             11153..11233
                     /locus_tag="VW006_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11743..11931
                     /locus_tag="VW006_000943"
                     /pseudo
     CDS             11743..11931
                     /locus_tag="VW006_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            12346..12963
                     /locus_tag="VW006_000944"
     CDS             12346..12963
                     /locus_tag="VW006_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9379817.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(13283..14170)
                     /locus_tag="VW006_000945"
     CDS             complement(13283..14170)
                     /locus_tag="VW006_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9379818.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(14319..16202)
                     /locus_tag="VW006_000946"
     CDS             complement(14319..16202)
                     /locus_tag="VW006_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9379819.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            16579..18033
                     /locus_tag="VW006_000947"
     CDS             16579..18033
                     /locus_tag="VW006_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9379820.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRTSEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            18014..18979
                     /locus_tag="VW006_000948"
     CDS             18014..18979
                     /locus_tag="VW006_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9379821.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKPAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(19014..20288)
                     /locus_tag="VW006_000949"
     CDS             complement(19014..20288)
                     /locus_tag="VW006_000949"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9379822.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(20288..21460)
                     /locus_tag="VW006_000950"
     CDS             complement(20288..21460)
                     /locus_tag="VW006_000950"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9379823.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(21591..21839)
                     /locus_tag="VW006_000951"
     CDS             complement(21591..21839)
                     /locus_tag="VW006_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379824.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPISQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(21907..22532)
                     /locus_tag="VW006_000952"
                     /pseudo
     CDS             complement(21907..22532)
                     /locus_tag="VW006_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(22594..23043)
                     /locus_tag="VW006_000953"
     CDS             complement(22594..23043)
                     /locus_tag="VW006_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9379825.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(23045..23803)
                     /locus_tag="VW006_000954"
     CDS             complement(23045..23803)
                     /locus_tag="VW006_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9379826.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(23918..25897)
                     /gene="pknB"
                     /locus_tag="VW006_000955"
     CDS             complement(23918..25897)
                     /gene="pknB"
                     /locus_tag="VW006_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9379827.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(25894..26634)
                     /locus_tag="VW006_000956"
     CDS             complement(25894..26634)
                     /locus_tag="VW006_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9379828.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(26649..27962)
                     /gene="rsmB"
                     /locus_tag="VW006_000957"
     CDS             complement(26649..27962)
                     /gene="rsmB"
                     /locus_tag="VW006_000957"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9379829.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(27955..28890)
                     /locus_tag="VW006_000958"
     CDS             complement(27955..28890)
                     /locus_tag="VW006_000958"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9379830.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(28903..31299)
                     /locus_tag="VW006_000959"
     CDS             complement(28903..31299)
                     /locus_tag="VW006_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9379831.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHARDVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(31365..31679)
                     /locus_tag="VW006_000960"
     CDS             complement(31365..31679)
                     /locus_tag="VW006_000960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9379832.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(31704..32330)
                     /gene="gmk"
                     /locus_tag="VW006_000961"
     CDS             complement(31704..32330)
                     /gene="gmk"
                     /locus_tag="VW006_000961"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9379833.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(32460..34073)
                     /locus_tag="VW006_000962"
     CDS             complement(32460..34073)
                     /locus_tag="VW006_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9379834.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(34143..34433)
                     /locus_tag="VW006_000963"
     CDS             complement(34143..34433)
                     /locus_tag="VW006_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9379835.1"
                     /translation="MEVEEKSIELSYEYVDDGYSGSNFERPNFKNMIDDLNKGKFSII
                     MVKDLSRFGRDYIESGKYLQKIFPEKGIRFISVNDNYDSDNADVSDIHLRFV"
BASE COUNT         9869 a         7793 c         6582 g        10287 t
ORIGIN      
        1 tagaaaatat tcacatttac ctaggaggaa caatatgaga tgggattatg gacaaatttt
       61 taaagaaatt cgaaagtcaa aaggattgac ccaacaagat gtatgtggac aagtcataca
      121 tcgaacaact ctaacaaata ttgaacacgg taaagttatt cccagttttg aaaacatggt
      181 atttcttctt gaacaaattg atatgagctt ggcagaattc aagtatatat gcaacgaata
      241 ccatcctagt aaaaggcgag atattattgt agagagccaa aatccgtcta cttttcaaga
      301 tactagaaaa atggttgaac tcactgagaa atgtcaaaaa tatcttaaga cacatcacga
      361 tgttcctatt caaaatatct atcgtcatac aaaaattgtc acagagttac gaactaaagg
      421 attcaaaaac aaccacgtct tgaaagattt gtatgaagaa atttgggact atcttgaacc
      481 tatggataca tggtacatta gtgatttgaa attgcttgga accattctct ttttctttcc
      541 ttctgaaaat cttccccttc ttattgatag aattatgaaa accatcgaga aatataaata
      601 cttccgagaa acaaaagcat ttttatcatc ttttttagcc aatctctcca ctgtatattt
      661 tcaacatcat ttattcaaag aatgtgaaac aatcacccta caacttttgg tattagcaga
      721 agaattaaaa atatacgata ttcttggatt ctctcaagta cgcttgggaa tactacaaca
      781 taactccgat ttgattgata aaggaataac tttattacga ctaaccaaag aagaggcatt
      841 agttaaaata ctcgaaaaag aaattaatga tttttcaaac ctataatttg taataactct
      901 catcatttta ttatagtaga ttgtaataga atgtaataga atgtgaacga atcaattagg
      961 ggatctaaag cattttatac caatgtttta taagttgtat tgtattattc catattcaat
     1021 ccactataca agatgaatat ttctctcaag tttcataatc aaaataaaaa tgactgtatc
     1081 tatagctcca taaacaaatc atagatacag tcatttttta gttcctacac cttcctttat
     1141 gcacaccttt agatttccct agttcccgcc tcaagcgaat catttttttg aaaaagacat
     1201 aggctaacca atcactagca aaagcgataa gtagtaataa tagccaaata ccccataact
     1261 tgatatctgt caaatttctc aagacgatat tgaaaaacag aactgaaaaa actgtccaag
     1321 caaggctaaa aagagcatag agggtgatgt aaatccagta aaaatagtaa aaaattggga
     1381 aaaatttact ttctctatta tccttgtcaa tccagctgaa aaagtaaaaa cagggtaata
     1441 agattatcat tttaaacaag tgttccatca caagcctcct tttttaaatt gttttcttta
     1501 gtttcgttct attaaatgtt attaccaata taaaccttgg aatagcgtgt aataaatgat
     1561 tttagaggta ccataagcca cctcctacaa atagaaaccg atataaatca atgccttcca
     1621 cccttagact tccctagttc ctgtctcaag cgaaacattt ctttgaaaca ggaataagtt
     1681 aaccaattca taccaatagc tagcagaata aaaagaaacc aaatgcccca taacttgata
     1741 tctgtcacat ttctcaagac ggtattgaaa aacagaactg aaacaactgt ccaagcaagg
     1801 ctaaaaagag aatagaaggg gatgtaaaac cagtaaaaat aataaaaaat tggaaaaaac
     1861 ttactatttc tgttggcctt ttcaatccag ttatcaaaat aaaagtacgg tgctaaaagt
     1921 aagaatttaa acaaatgttc catcaccgac atcccccttc ttttgatagc gttttctatt
     1981 attttattat atcaaaaaaa tccggaactg tcattccaga ttctactttt ttatttgcgt
     2041 tttcttgcga tgagatgaat cggtgttccc tcaaaaacaa aggccttgcg gatttgattt
     2101 tccaagaaac gcaggtaaga aaagtgcatg agttcttctt cattgacaaa gatgacaaag
     2161 gttggtggtt tggttgccac ttgggtcgca tagaaaatct tgagacgttt tcctttgtct
     2221 gtcggtgttg ggttgatggc aatggcatcc atgatgacat cgttcaagac agctgatgga
     2281 atacgtgtat tttgactttc gctgatttgc ttaatcatct caggaagttt gtggagacgt
     2341 tgcttggtta aagctgatac aaagataatc ggtgcgtaag gcaggtattg gaactgctca
     2401 cggatatctt cttcccagtt tttcatagtg tggttatctt tttcaagcgt atcccacttg
     2461 ttgaccacga taatcatccc tttaccagct tcatgagcaa atccagcgat acgcttgtcg
     2521 tattcacgaa tcccctcttc cgcattgatg accattaaga ccacatctga acggtcaata
     2581 gcacgcatgg cacgcatgac agagtacttc tcagtatttt cataaacctt accagactta
     2641 cgcataccag ccgtatcaat catggtaaac tcttgaccat ctgtatctgt aaagtgggta
     2701 tcaatagcgt cacgagttgt tccagcaaca ggactggcaa tgacacggtc ttctcccaag
     2761 atagcattaa tcaagcttga ttttccaacg ttaggacgac caatcaagct aaacttaatg
     2821 acatctggat tttcttcttc atattcattt ggaaggtttt ccacaatcgc atctagtaca
     2881 tcccctgtac cgataccatg gacagatgag ataggcagtg gttcacccaa accgagagca
     2941 tagaaatcat agatatcatt tctcatctca gggttgtcca ccttgttgac tgcgaggata
     3001 actggtttgt gggtcttata aagcttacga gctacgtatt cgtctgcatc agtaattcct
     3061 tccttaccag acacgacaaa aacgataaca tctgcttctt ccatggcaat ttctgcctgg
     3121 tgcttgattt gttccatgaa aggagcatcg acatcatcaa ttcctcctgt atcaatcatg
     3181 ctaaaagaac gattgagcca ctcacccgtt gcataaatac ggtcacgtgt cactccttcg
     3241 acatcttcta caatggagat tcgctcacca gcgatccgat taaatagggt tgatttccca
     3301 acattgggac gtcctacaat ggcaatagtt ggtagggcca taatttctca ctttctacaa
     3361 taatttcttc tgttcaagat tttttctagt tgagcttggt tcagcttgac caaactgttc
     3421 tgctaggcgc tgactccagc ttgtggtcgc acgcgcccca gcatagtcag cctgaacacg
     3481 gtcataagct tggattgcct cagttgactg ttcttggtat tcttcctcaa agacaacatt
     3541 ctctagtggc agtctcggtt tcatatcatg atgttgattt ggcacaccca gtgccatccc
     3601 aaagacagaa taggtgtagt caggtaggtt aaagagctct gccacttctt cagacttgta
     3661 tcgaaccaaa ccgataatca caccaccata gcccaagctt tcagctgcca acaaggcgtt
     3721 ttgtccagca agagctgcat cgaccgaact aatcaagaga ccttccacac cttggggttg
     3781 gaaggtgtcg gtatgaagtc tggctccctt ttctgctcgg ttcaaatctc cgacaaagag
     3841 aaggaaaaca gcagactggc gaatggcttc ttgaggtacc aattcataca aggcatcttt
     3901 cttctcttga cttcgtacca caatcacaga gtaggattgg aaattcttcc aagatgatgc
     3961 catctgggct gctgtcaaaa tctcatttaa gtctacttgg ggaatttctt gctctttaaa
     4021 cctgcgcact gaagtatgag ccttcatcaa tttaatggtt tctgtcatcg acggtttact
     4081 ccttctaaac gagtctcctc agccaaataa cggatgcgtt ccatgacccg tctggcttcc
     4141 caggtttcgt catttccatg tttcactttc gcaaaatgct tctccaaatc ttcaaagttg
     4201 aagttggatg tgaaaaaggt cggtaaattt tcctgcatcc gatattggag aatgacctgc
     4261 aggatttcat cacgcaccca agcggtggat tgctcggcgc caatatcatc taaaatcagg
     4321 acctcagaca gcttaatctc atccaccaag gtcttaacat tgccatcact gatagcattt
     4381 ttgacatcaa tgacaaagct aggatagtgg aggagagttg atgaaacacc acgtttttct
     4441 gataaatcat gagctaaggc cgccaccatg aaacttttac ccacaccaaa gtctccatat
     4501 aagtaaagac cttttcgaat agctggatat tgctccacga aggctagtag cttttcaaaa
     4561 actggtaagc gccccaaatc atccaagtca acttgagcca aactagcttt cttgagactg
     4621 gctggtagat tgattaactt gagacggttc ttaatagccg cttctttttc agccgcgatt
     4681 agctcaggag tttcttcata tgaaacatct gcatagccat gattcttaac caaaatcggc
     4741 ttgtagcctt tggcaatata atccgtatcc ccacggagaa acttgtcacg ctcggtgatg
     4801 tactgattaa acttggagat actgcgattt aattcctctg gagtcaagga ttcttgctgg
     4861 ataaaagccg caacatcagg gtccttcatg attttctgga ccaaatcttg ataataaaaa
     4921 cggctgggtt gacgtttgag tacgtctccg acactttcca tctaatctcc tcctttttct
     4981 aatcgagcta atagttcttg cttcttacgt tctagttcca gacgagtttc ctcgctggtt
     5041 tcattcttat attcaggatt actccattta ggaacattgg tttttactgg ggcagtctga
     5101 ttctgttttt gtgtttttgc tttctgtcct cgctcacgga tgcgcaagac tgccttttct
     5161 gccgaatgaa tcttttgata ggcatagtca ttggctacct tcatggcata tttctcattg
     5221 atatttgccg aatcgacctt attaaaggtc aataaaagaa tgatattgat gacttcgtcc
     5281 agcaagccca agccagccat ctgttgcaag agttctcttt ctgtttgggt aatggttccc
     5341 ttgcgtgttt gcttgatttc tgccaagaac tgcagggcag ttttactttt ggcttctttg
     5401 ataatggttg tttccttaag actaaagtca gaggaaactg gtttttgagc aattttttca
     5461 cgcatgcgtt tggttgaaat aacctgagaa acagctgttg acttggccaa ttgataggtt
     5521 tcaaaccaag tccatttctt ctcctcggca atagcaaaga ggtttaagac atcggactgc
     5581 tcatccgcaa aacgaagtcc atctcgagcc atcagctggc gaaaatgttc caagtcaaaa
     5641 tcattggcca ctttcttctt gagaccaagg tcttcttgac tgcctagttc tgccaattct
     5701 ggaaagactt gattgagtga gacaggtatt tcttcaccat cagcactttc aactttcaaa
     5761 tcctccacag cagagtcgcc aatctttttc tctaagagtc tgcgataaac aggatgctcc
     5821 aagaagtctt gactagatag aggagcatgg agggctagct gataaacatc ccccttttga
     5881 tagagggtca agagattaaa agcagataag attttcaatg attttatcag tctatccatc
     5941 ccaaagttga gatgattgag gatacttgaa aaaagatatt cctttctacc attatcccaa
     6001 aaactgattg tataaagata aaggctcagt gcctcctgac cgataatcgg gaggtagcac
     6061 tgtaccagag atgaggtatc ttgcgacacc cgattattct ttagataaga aaaacggtca
     6121 attggcttca tttatctttc ctttttcttt ttagaggact gggtgatttg ttggagcaag
     6181 ctctctaact cactgacatc cttaaaacta cgatagacac tagcaaaacg tacataggta
     6241 atctcgtcca attcagccaa ctcctccatg acgagtgaac caatgtcctc actttgaatt
     6301 tcattttcat ttcgaccacg gagtttctgt tcgatacgat tgactaccat gttgatttca
     6361 tcacttgaca caggacgttt ctgggctgag cggataatcc cattaaagat tttatctctg
     6421 gagaattgtt cccgtgtgcc atctttttta acaaccacta aggttctttc ttctactcgt
     6481 tcgtaggttg taaaacggtg ttggcactcg tcgcactctc gtcttctacg aatggtgttc
     6541 ccttcttctg cttggcgact atcgataaca cttgacttgg tagccccaca ttttggacaa
     6601 cgcatccttt ccctccttat cgttttcttt tcattatacc attttttaag cgattcccaa
     6661 aacaattctt ctttttgctt gacaagtttt ttgttttgtt gtattattta attaagacaa
     6721 aaggataaga gaaaggagac taagatgtcc tggacatttg acaacaaaaa acccatctat
     6781 ttacagatta tggagaaaat caagcttcag attgtttccc atacactgga acccaatcaa
     6841 caacttccaa ccgtgaggga gctagctagc gaggctggtg tcaatcccaa taccatccaa
     6901 agagccttat cagaccttga acgagaagga tttgtctaca gcaagcgaac aactggacga
     6961 tttgtgacta aggataagga gctaatcgcc cagtcacgca aacaattatc agaagaagaa
     7021 ttggaacact tcgtttcctc catgacccat tttggctatg aaaaagaaga actaccaggc
     7081 gtagtcagtg attatattaa aggagtttaa gcctatgtca ttactagtat ttgaaaatgt
     7141 atccaaatca tatggagcaa caccagccct tgaaaatgtt tctcttgaca ttccagctgg
     7201 aaaaattgtc ggccttcttg ggccaaacgg ctcaggaaaa acaaccctga ttaaactaat
     7261 taatggcctc ttacaaccag atcaaggacg tgtcctcatc aacgacatgg acccaagccc
     7321 agcaaccaag gccgttgtag cttatttgcc tgatacgacc tatctcaatg agcaaatgaa
     7381 ggtcaaagaa gccctaacct acttcaagac cttctataaa gatttcaatc ttgaacgcgc
     7441 ccatcatcta cttgcagacc tgggcattga tgaaaatagt cgtctcaaga aactatcaaa
     7501 aggaaacaaa gaaaaggttc aactgatttt ggttatgagc cgtgatgctc gtctctatgt
     7561 tttggacgaa cccattggtg gggtggatcc agcagcccgt gcttatatcc tcaataccat
     7621 tatcaacaac tactcaccaa cttctaccgt tttgatttct acccacttga tttctgatat
     7681 cgagccaatc ttggatgaaa ttgtcttcct aaaagacgga aaagtcgtcc gtcaaggaaa
     7741 tgtagatgat attcgctacg agtcaggtga atccattgac caactcttcc gtcaggaatt
     7801 taaggcctaa gcaaaggaga ttatttatgt tttggaattt agttcgctac gaatttaaaa
     7861 atgttaacaa gtggtattta gccctctacg cagccgtgct agtcctttct gccctcatcg
     7921 gaatacagac acaaggcttt aaaaatctac cttaccaaga aagtcaggct actatgctac
     7981 tttttctagc tacagtcttt ggtggcttga tgcttacact tgggatttca accattttct
     8041 tgattattaa acgcttcaaa ggtagtgtct acgaccgaca aggctatctg actttgacct
     8101 tgccagtttc tgaacaccat atcatcacag ccaaactaat cggtgccttt atctggtcat
     8161 tgattagcac cgctgtattg gctctaagtg ctgttattat tctggcttta acagctccag
     8221 aatggattcc tctttcttat gtgattacat ttgtagaaac acatctccct cagatctttc
     8281 ttacaggtat atccttccta ctaaatacta tttcaggaat cctctgcatc tacctggcta
     8341 tttccattgg acagcttttc aatgaatacc gtacagcact cgctgttgca gtctacattg
     8401 gtatccaaat cgtcattgga tttattgaac ttttcttcaa tcttagttct aatttctatg
     8461 tcaattcact ggtaggactc aatgaccatt tctatatggg agcaggtata gccattgttg
     8521 aagaactcat attcatagct atcttttatc tcggaaccta ctacatcttg agaaataagg
     8581 ttaatttgct ttaaataatt tttacctaga tatgtaacat actcatagaa caaaagagac
     8641 caggcaaaaa gtctttacaa ttagaaaacg catagtatca agtgttaaaa atccttgata
     8701 ttatgcgttt tttgtgagct gacttatttc ctttcactat atcgcaaaat gaaataagaa
     8761 cggaacgatg ggattttgga attcaaatca atttataaga atgttttaga agtaatatta
     8821 tcctattcca gattcagttc actatacaat tgagttttca agcaacctgt ttacataatg
     8881 tgtacataat taggttcgtg attccaccct tttcaccttt aaaaacctcg ctttcgcaag
     8941 gctcttctat ttataagata aggcacgttt aaaggttttc caaatcccta aatcatccgt
     9001 ttgaagaacg agactagcat acatgcgtcc gataaatcct gttgctacca ccgcaaaaat
     9061 cactgtaata gcaagtgaaa tccatgcttc cgctccctcc gcatagccat taatcgttcg
     9121 aaacggcata aagaaggtcg aaataaaggg aatataagaa ccaatcttca agaggagatt
     9181 gtcaccagct gcacctagag ctgtcactcc aaaaaaacca cccataatca aaatcatcaa
     9241 aggcgacaag gctttccctg agtcttcagg acgagaaacc atagatccta ggaaggctgc
     9301 caagactacg tacatgaaaa gactgatcaa aataaagagc aaggtattca gtgagatagc
     9361 atctcccaag tgatccaaaa taccagactg agccaagaat ggcaaatctt taaagagcaa
     9421 aacggcagcc agaccaccta caacatagat cccaatatgc gttaaaatca ctagaaacag
     9481 agccatcatc cgcgcataga aatagtgact tgcccttatg ctagaaaaga cgacttccat
     9541 aattttggtg cctttttcac tggcaacttc ctgagctgtt acacccgcat aggtaatcag
     9601 aatcatataa agaaagaatc ctaaggcacc tgctgcaatt gtttgaataa actttttatt
     9661 ttccttggct tcatcaatct tttctgtgaa ttgaattgtc tgcgctaagc gtttttcctg
     9721 ctcttgagac aaggaagcag ttgaacgatt aagctgattt tgcagttcat tgagtgtacc
     9781 tgtaacctca aatttaattc cattttcaag cgatgtttcg ccatgataaa ctgcctttag
     9841 aacactatct tcttgatcaa tggtcaaata accttttaat ttttcttctt taattgcttc
     9901 tttggcactt gcttcgtctt tatagtcgaa gttaacacca tttacattct tcagtccttc
     9961 tgctacagat ggcactgttg tcactactgc cactttatta tttttagcca tagaagaacc
    10021 ttggagatgc ccaattccta cagagattcc taaaaagagg aacggcgaaa tcaccataaa
    10081 gaagaaactc catgactcga catgtcgaag ataggtttcc ttgattacaa cccacatatt
    10141 tctcatactt ccactcctga ttctagttta aagatttcat cgatagttgg cgcttgttgg
    10201 tcaaatgttg cgatatattg accttgagtc aagattggga agagttccct tccagcgctc
    10261 tcatcctcca aaatcaattt ccaactgcct tgtttggtca agctcacctg tttgacatga
    10321 ggaagatttt ccaattcttc cttgcttcgt tcacttgaaa caaagagacg cgttttcccg
    10381 tattgattgc ggacatcctg aactggtccg tgcaagacca cacggccatc tcgaatcatc
    10441 agaatatcgt cacaaagttc ctcaacattg gtcatgacat ggtcagaaaa gataatggtt
    10501 gctccgcgct ctttttcctg aaaaatgact tgtttgagca attctgtatt aactgggtcc
    10561 aatccactaa aaggctcatc caagataatc aggtctggtt catgaatcag agtaataatg
    10621 agctgaatct tctgctgatt tccttttgac agactcttga ttttatctgt cagctttcct
    10681 tttacttcca acctcttcat ccattgaggg agtttttctt tgacttcttt ggcatccatg
    10741 ccttttagag tcgccaagta gcgaacttgt tcaagaactg tcaatttagg catgagactg
    10801 cgttcttcag gcagataacc aatccgagca tagatctcct gacgaatatc ctgaccatcc
    10861 agaccgattt ctccctgata ttctaggaat tttaaaatac tatggaaaat cgttgttttt
    10921 ccagcaccat tttttccgac tagtcccaaa atacgacctg gtcgcgcttg aaagtcaata
    10981 ccaaacaaaa cttgcttgga tccaaaactt ttctctagac ttcttacttc tagcatcttt
    11041 cacctccgaa atttcttgca ctcattatac tcctttttga tagcctttac aatgtttttt
    11101 gtccattttt agaagactat tgctgtgtaa aatatggcct ggagcacttt tatactcaat
    11161 gaaaatcaaa gagcaaacta ggaagctagc cgtagactgc tcaaagtaca gctttgaggt
    11221 tgcagataaa actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga
    11281 cgaagtcagt aactatacgt acggcaaggc gaagctgacg tggtttgaag agattttcga
    11341 agagtattag tgataaatcc attatacagc agcaaactta atttatacct tccgctcctc
    11401 aactgtctat ttttaatcct gaattgttat ttgagtaact cctttttcct cgtaaagttt
    11461 tcttcctcta aaacttctgg aaaaaggcta atagtttcag acaacatttt tataagaaac
    11521 aagttcatct gtcatttcaa gaaggagtaa tcctttatct actaatggac ggaacagaat
    11581 tcaaccgctt gtccgatatg ttttctaagg attatatagt aaaatgaaat aagaacagga
    11641 caaattgatc aggacagtca aattgatttc taacaatgtt ttagaagtag atgtatacta
    11701 ttctagtttc aatctgctat atctattatg cacaccccta taggatctaa tgaaaatcac
    11761 aacaggctca ttcatagata gttacctcag gaaactaaga aaacgactac aaaggaagtc
    11821 gcattcatcg aaaagtggat taacaactat cctaaaaaat gcttgaacta caagtccccc
    11881 agagaagact tctggatgac taacttgaac ttgaaattta gcaataatta attcaccatc
    11941 taactatatt tagtaattat ttcagaactg attaatatta aaattaacta acaattcaaa
    12001 ggattcatac tagccataaa ttacgtccat cagagagaga ctcttactac ttttagattt
    12061 tagtctttct agcttcagaa tacatctaaa ctttagggaa aatgactatt cgaaagcgcg
    12121 aatgcctcaa aattatctca gataagctat tcgaaactta gaatgctttt aaatttatgg
    12181 aattgcgatt attcgaaacc tagaatgcat ataaccttta gttgacagac ctattctaag
    12241 tctcgaaggg ctatttactt tctattcctt atcaaaaaag actcattccc cctttctcct
    12301 ccaaaatatg gtatagtaga aatatactat ctatgaggag tttacatgtc acaggataaa
    12361 caaatgaaag ctgtttctcc ccttttgcag cgagttatca atatctcatc gattgtcggt
    12421 ggggttggga gtttgatttt ctgtatttgg gcttatcagg ctgggatttt acaatccaag
    12481 gaaaccctct ctgcctttat ccagcaggca ggcatctggg gtccacctct ctttatcttt
    12541 ttacagattt tacagactgt cgtccctatc attccagggg ccttgacctc ggtggctggg
    12601 gtctttatct acgggcacat catcgggact atctacaact atatcggcat cgtgattggc
    12661 tgtgccatta tcttttatct agtgcgccta tacggagctg cctttgtcca gtctgtcgtc
    12721 agcaagcgca cctacgacaa gtacatcggc tggctagata agggcaatcg ttttgaccgc
    12781 ttctttattt ttatgatgat ttggcccatt agcccagctg actttctctg tatgctggct
    12841 gccctgacca agatgagttt caagcgctac atgaccatca tcattctgac caaacccttt
    12901 accctcgtgg tttataccta cggtctgacc tatattattg actttttctg gcaaatgctt
    12961 tgacacgtaa aaaatccgtt tggtttccca agtggatttt taaagcgtag attaactata
    13021 gcttgatact aaatatactt tggtatggaa atcatgcata tttttcgata gtgaagcgag
    13081 gacttaccta gcctttccac cgtgatagaa acacctgaaa tctaatggtt tcaggtgttc
    13141 ggaaactttg agcctagtgt ctcaaagttt aggtatggaa tttcgaagaa agtcgctacc
    13201 gtccgtaatc acttaaggaa aggctcaaaa atattgtttt caaccacaaa atccgtttgg
    13261 tttcccaagc ggattttgtg ctttattttg aaacttcttt tgcaagaaca aagttcccaa
    13321 gtgtggcaga accatttcct gcgactgctg gcgtcacgat atagtcacgc acatctggta
    13381 ctggtaggta accattaaga agagatgtaa atttctcacg gacacggtcc agcatatgtt
    13441 gttgagccat gacccctcca ccaaagacaa tcacgtctgg gcggaaagtc actgtcgcat
    13501 taaccgcagc ttgagcgata tagtaggctt gaacatccca aacagggttg ttgagttcaa
    13561 tagtttcccc acgtacacct gtacgagctt ccaaacttgg accagctgca tagccttcca
    13621 gacatccctt atggaaagga caaacaccct taaactcttt ttcaatatcc attgggtgtc
    13681 tagcaacata ataatgaccc atttcagggt gacccacacc accgataaac tcaccacgtt
    13741 ggatgacgcc tgcaccgata cctgtaccga ttgtgtagta aaccaagttt tcgatacgac
    13801 caccagcatt gttacgggca accacttcac cgtaagcaga gctgtttacg tctgttgtga
    13861 agtacattgg cacgtttagg gcgcgacgaa gggcaccaag caagtccaca tttgcccagt
    13921 ttggttttgg agtcgtcgtg ataaagccat aagtttttga gtttttgtca atatcaatcg
    13981 gcccaaatga accaactgca agaccagcaa ggttatcgaa ttttgagaag aactcaatgg
    14041 ttttatcgat tgtttcgatt ggagttgttg ttggaaattg tgttttttct acaacgttaa
    14101 agttttcatc accgacagca cagacaaact ttgtaccgcc agcttccaag cttccatata
    14161 attttgtcat gataaacctc ttgtttttat tttctttatt atagcatact tcgaaagtct
    14221 aaatgtctct attttttaga ttttcctctg caaatcttac tatctaataa aaacgaacaa
    14281 acatgtcatt tgttcgtttt cacattagag aggattgatt agattttcac ttcgatcaca
    14341 gcatccccct tcgcaactga acctgttgcg actggagcta ctgaagcgta gtcagctgta
    14401 tttgtaacga taaccattgt tgtatcatca agtccagctg tagcgatttt atttgagtca
    14461 aatgttccaa gaacatcgcc aactttcacc ttatcacctt gagcaacttt tgcttcaaaa
    14521 ccgtcaccgt tcatagatac agtgtcaata ccaacatgaa tcaaaacttc agcaccattt
    14581 cttgttttca aaccaaaagc gtgccctgtt ggaaaggcaa ttgaaacttc agcatcagct
    14641 ggtgcataga ccacgccttg gcttggtttc acagcgatac cttgtcccat agctccactt
    14701 gagaagactg ggtcattgac atcagcaaga gcgacaacat caccgacgat aggagttaca
    14761 agtgtttcat tttgaagagc tgctggcgca acttcttctt tttcttcagc cacttcagct
    14821 tgttttgcag ctgcagttgc gtctacttca tcttcgtaac caaacatgta agtaagagca
    14881 aaaccaaggg caaatgatac agctaccata agaaggtatt gtggaagttg tccgttacca
    14941 acataaagca ttgtaccagg gatgatggtg ataccattac cagtaccagc aagtccaagg
    15001 atagaagcca atccaccacc gattgcacca gcaatcaatg aaaggaagaa tggtttacgg
    15061 aagcgcaagt tcaccccgaa gatagcaggc tctgtaatac ctaggaaggc agaaagagca
    15121 gccgggaaag caagtgtttt cagttttgga ttttttgttt taacaccaac cgcaacagta
    15181 gcagcacctt gagctgtcat agcagctgtg atgatagcgt tgaatgggtt agcatggtca
    15241 gcagcaagta attgcacttc aagcaagttg aagatgtggt gcacacctga cacgacgatc
    15301 aattggtgaa ccccaccaat caagaaacca ccaagaccaa atggcatgct aagaatcgct
    15361 tttgtagcaa taaggatgta gttttcaaca acgtggaaaa ctggtccaat gacaaagagt
    15421 ccaaggatag acatgaccaa aagtgtcacg aatggtgtta ccaagaggtc aatgacatct
    15481 ggaacaacct tgcggacagc tttttcaaat ttagctccga caaccccgat gatgaaggct
    15541 ggaagaacgg aaccttgcaa accaacaaca gggatgaaac caaagaagtt catcgctgtt
    15601 acttcaccac cttgagcaac tgcccaagcg tttggaagtg agccagagac aagcatcata
    15661 ccaagaacga taccaacggc aggatttcca ccaaatacac ggaaggttga ccacacaacc
    15721 aaacctggca agatgatgaa ggctgtatct gtcaagattt gtgtgtaagt tgcaaagtca
    15781 cctggaagtg gcatttcaag agcgttgaaa agaccacgca cacccatgaa gagacctgtc
    15841 gctacgataa ctgggatgat tggaacgaaa acatcaccaa aagtacggat agcacgttgg
    15901 aaccagttcc cttgtttagc aacttctgct ttcatgtcat ccttagatga tgttggtaat
    15961 ccaagtacaa caacttcatc gtacattttg ttaactgtac ctgtaccaaa gataatttgg
    16021 tattgccctg agttaaagaa agcaccttga actttttcca agttctcaat cacttcttta
    16081 ttgattttct cttcatcttt gaccatgaca cgtagacgtg tcgcacagtg ggcaacacta
    16141 ttgacatttt cacgtccgcc caaggcatcg atgacttttt ttgcaatttc ctgattgttc
    16201 atttgcaaaa atctccttat ataacatttt gttcttgttt gaaagcgatt ttattcgccc
    16261 ttacgactat tattttatca tgttttaaaa atatgtcaag cgttttgcag aaaaaatatt
    16321 ctatctactt agtgggcaag ctttagattg attgtttttg actgtttcat caggaatttc
    16381 ttgaaaaata agtttgaaac cttggttaaa aagtctttta tttcattatc tttcacattt
    16441 tcgtaaaaat ttgattgatt tcctgtattt attttatgat aatcgtttga cagttttttc
    16501 tcttttttaa cataaagggc ttgcattttt tatcgaaaac agttactatt aaaagtgata
    16561 agatttttgg aggaatgaat ggaatggaca actgagcgtc gttacagact ttatcaagat
    16621 tggacgcaag aagaaattca acatataaag gaaaatatgg cacaatctcc atggcatact
    16681 cattaccatg ttgagccaaa aacaggactt ctcaacgacc caaatggctt ttcttacttt
    16741 gatggcaagt ggatcctctt ttaccagaat tttccttttg gtgcagccca cggtttaaaa
    16801 tcttgggcac agctagaaag tgatgatttg gttcacttta gagaaactgg agtcaaagtt
    16861 ttgccagata ctccattaga tagtcacggt gcctactctg gttctgccat gcaatttggc
    16921 gataacttat tcctatttta tacaggaaat gttcgcgatg aaaactggat ccgtcaccca
    16981 taccagatcg gtgctttgat ggacaaggag ggtaagatta caaagattga caagatcttg
    17041 attgaccagc cagcagactc tactgaccac ttccgcgatc cacaaatttt taactttaaa
    17101 ggtcaatact atgctgtcgt cggtggacag gacttggaga aaaaaggctt cgtccgtctc
    17161 tacaaggctg tcaataacga ctacacaaac tggcaagcag ttggcgacct tgactttgct
    17221 aacgaccgta ctgcctacat gatggaatgt cctaatctgg tctttgtaga ggaacagcct
    17281 gtccttctct actgtccaca aggattggat aagaaagttc tagactacga taatatcttt
    17341 ccaaatatgt ataagattgg ggcttccttt gaccctaaaa atgccaaaat ggtagatgtg
    17401 tctcaacttc aaaacatgga ttacggtttc gaagcctatg caactcaagc cttcaacgct
    17461 cctgatgggc gtgctctagc agttagctgg cttggtttgc cagatgtttc ttacccatct
    17521 gaccgttttg accaccaagg aaccttctct ttggtcaagg aactcactat caaagacgac
    17581 aagctctacc agtatccagt cgctgctatt aaggaccttc gtacttctga agaagccttc
    17641 tcaaaccgtt cccaaaccaa gaacacttac gaacttgaac tcaacttgga agctaatagc
    17701 cagagcgaga ttgtcttact tgctgataaa gaaggtaagg gactttcaat caactttgac
    17761 cttgtaaacg gtcaagtgac agtggatcgt agccaggctg gagagcagta tgcccaagaa
    17821 tttgggataa ctcgttcttg ccctatcgag aatcaggcta ctactgctac aatcttcatc
    17881 gataactctg tctttgaaat tttcatcaat aaaggagaaa aagtattttc tggtcgtgtc
    17941 ttcccacatg cggaccaaaa tggtatcctg atcaaatctg gaaacccaac tggaacttac
    18001 tatgaattag attatggtcg caaaactaac tgatgtcgcc aaacttgcag gcgtcagtcc
    18061 tactaccgtt tctcgggtta tcaataaaaa aggatatcta tctgagaaaa ccatccaaaa
    18121 agtcaatgaa gccatgcgag aattgggcta taaacccaac aacctggctc gtagtctgca
    18181 aggaaaacca gctaagttaa tcggcttgat tttccccaat atttccaatg ttttctatgc
    18241 agaattgatt gataaattgg aacaccaact cttcaaaaat ggttacaaga ccatcatctg
    18301 caacagtgaa catgattctg agaaggaacg tgaatacatc gaaatgttgg aagccaatca
    18361 ggtggacggc atcatttctg gtagtcacaa cctaggaatc gaagactaca atcgtgtgac
    18421 agcgccgatt atttcctttg accgaaacct atcgccagac atccctgtcg tctcctctga
    18481 caactatgct ggtggggttc ttgctgccca aaccttggtc aagacaggtg cccagtctat
    18541 catcatgatt acagggaatg acaattccaa ttcgccaacc ggactgcgcc acgctggttt
    18601 tgcatccgta ctcccaaaag ctcctattat caatgtttcc agtgactttt ctcccgtcag
    18661 aaaagaaatg gaaatcaaga atatcttgac ccgggaaaaa ccagatgcca tttttgcttc
    18721 ggatgatttg acagctattc tggtcattaa aatcgctcaa gaattgggca tttctgtccc
    18781 aaaagagctc aaggtcatcg gctatgatgg gacctacttt atcgaaaatt actaccctca
    18841 attggctact atcaagcaac ctttggaaga gattgcttgt ctcactattg atcttctctt
    18901 gcaaaagatt gaaggcaagg aagtcgccac aactggttac ttcttaccag ttacgctatt
    18961 accaggaaaa agtatttaaa cacaagaaaa ctcagaccga ttcgtctgag tttttatgat
    19021 cttaaatttt cgagatagcg ctgggctgtc tctaggttaa aggttttatc tgagatgagg
    19081 cgctctacta ggggagcaac ttcagattca ctagccccag ctaggagagc tagggatttg
    19141 gcctgtagtt tcatgtggcc ttgctggatg cccgtactta ccaaggcttt gagggctgca
    19201 aaattttgag caagaccgat ggacacgata atctgggcta attctctggc agaaggattt
    19261 cctagtagat catgactgag agctacacgt gggttgaggc cgatagagcc acccttagtc
    19321 gctacaggca tgggcagggt catctcaccg accaattctt ctctttcaag gtccagcgtc
    19381 cagcagctaa gaccttgata gcgtccatct cgactggcaa aggcatgggc cccagcttcg
    19441 atggcacgcc agtcattacc agtggcaatc aaaatcgcat caataccatt aaaaattcct
    19501 ttattatgag tagcagctcg gtaaggatca gcctgcgcaa actgactagc caacgcaatt
    19561 ttctccgcaa tctctcgtcc ttgatccttt tggcggctca agtagcgaaa ggcgatgcga
    19621 cagcttgcag tcaccagaga atcagtcgcg tagttggaca ggattcccat gagactctgt
    19681 ccctgactga gttcttctaa gactggtttc aaggcttcca gcatggtgtt gagcatattg
    19741 gcacccatgg cttcctgggt atcgacatga atataaacaa cgagaaagtc tggttcgcct
    19801 tttatctgct cgacatgcag atcacgcgcc ccacccccac gtttaacgat agaaggatag
    19861 gcttgattgg caagctccaa gagctccgct ttcttgctgg caatcttctc ttgcgctagt
    19921 ttaggattag caacttgata aagggctacc tgcccaatca tctggcgctg atggacttgt
    19981 gcagtaaaac cacctgcacg cttgatgatt ttgctggcat agctggccgc cgcaaccaca
    20041 gagggttctt ctgtcacata gggaacggtg tattcctgac cgttgacaag tacctccgga
    20101 accagcgaat aaggcagaga aaaagttccc accacattct cactcagctg gtctgccaca
    20161 gtcacactca tctgttcatc cttctccaga ctagcttgtc tctcaggact aaggagcgcc
    20221 tgagctttta gcagctcgag gcgctcttgg tatgattttt tagaaaatcc attccaactt
    20281 atcttcatta tttttcaacc ttgctataac ggcgttggtg gtcaagaatt tcaaccaagg
    20341 caaaatcttg attttcatag ccagcaaact gggcagagtt tgtttcatcc aagtttactt
    20401 cctcaaaaaa gaccttttca tagtctgcaa cggatagggc agttcgttgg ttgagcttgt
    20461 tcaagcggtc tttatccaaa taagcttcat atccttcaac caattcacca ctgaagaact
    20521 cagccacagc tccacttccg taactataaa gggcgatttt atccccagct ttcaagctat
    20581 ctgtattttc caagagagac aaaagtccaa ggaaaagtga acctgtgtag atattcccca
    20641 ccttttgact gtagagaata gactggtcaa aatgcttttg taagaggtct tttttctctt
    20701 gaggcaggct cttatccatg atttttttca agccttttag cgctaattta ggataaggca
    20761 agtggaaaca aacagccgca aaatcatcca aagtaagctg gtagcgtttt tgatattcaa
    20821 gccaagtcgt tttcaaacta tccaagtatt gttgggtaga atagacacca tttacataag
    20881 gagttgtcga gtaatttggt cgccagaaat ccatgatgtc acgggtctga gctacattgt
    20941 cattattaaa ggccatcatg cgtggatttt gtgtaatcaa catagctaca cttccagcac
    21001 cttgagttgg ttctcccgga gtttcaatac catatttggc aatatcactg gcaatgacca
    21061 agaccttgga ctccggagaa ttttccacat gcaatttggc ataatggagg gcagctgtcg
    21121 ccccatagca ggcttcttta atctcgaaac tacgagcaaa gggctggatg cccagcaagc
    21181 catgcacaaa gacggccgca gccttactct ggtcaattcc tgactcggta gccacaatga
    21241 ccatgtcaac ttcttgtctt tcttgctcag ttaaaataga gtcactagca ctggccgcca
    21301 aggtcacgat gtcctcagtt aggggcgcaa tactcaattc cttgagtaag agtcctttac
    21361 ttaatttttc agggtcaatt cccctcgctt ctgctaagtc ttgtaatttc aagacatatt
    21421 gactggtcgc aaaaccaatc ttatcaatac cgattgtcat atttacctct gttttatcat
    21481 tcatgtaaaa aatcgttcta tactatttta tcacaaatgg cagtaaaaga gagaaaaaag
    21541 acttgattca ccaaatcaag cctcttattg gtcatcattt taaagaatga ttagttgcta
    21601 gagagttcac cgatataagt agctttataa gctccattca cagttatcag ctcctggagg
    21661 atcaaatttc ctgagtaaat ccttcccatc tcatctacaa atttttgata aaactgactg
    21721 atcggaattt ctctgacatc cttatcaaat gtcttatcaa gtgttttact aaccttctca
    21781 gcaatcaatt gatgctcttg ccatccactt tgaaactctg agcccgaact agaaaccatg
    21841 actgggataa acaacaaggt cagtagattt acagacaata aggaaagtag tagacttcct
    21901 gcaaaactag aatcctagtt catgattgat aatgccagca atcaaattca ttcgtaatcc
    21961 gaagcgttta cgatgatttc gataggttgt tgaaaacatt ttaaacgttt ttactttggc
    22021 aaagatgttc tcaaccttgc ttctctcctt agatagcgca tggttacagg ctttatcttc
    22081 aactgttagc ggcttgagtt tgctggattt acgtggagtt tgtgcttgag gatatatctt
    22141 catgagccct tgataaccac tgtcagccaa gattttacca gcttgtccga tatttctgcg
    22201 actcattttg aacaacttca tatcatgaca atagttcaca gtgatatcca aagaaacaat
    22261 tctcccttga cttgtgacaa tcgcttgagc cttcatagcg tgaaatttct ttttaccaga
    22321 atcattcgct aattgttttt ttaggacaat tgatttgtac ttccgtcgca tcaatcatta
    22381 ccgtgtcttc agaactgaga ggagttcttg aaatcgtaac accacctttg aacaagagtt
    22441 acttcaaccc attggctccg acggagtaag ttgctttcgt ggataccaaa atcagccgca
    22501 atttcttcat aagtgcggta ttctcgcaca tatagaaagc gttgttaatt tatttatctc
    22561 atttttcaga aaattctttt atttctgtaa agtctacgat actcgatgtg tttttatata
    22621 atgatacagt ctgagaatca ctgttccgct agccattcca atagagatta ccaaagccaa
    22681 catgacaacc aaggtcgcac ttgccagtgc tttattatag tcccctgtca caaaaaaggc
    22741 agttgttcgg taggagagat aacctggaac cagcggtgcc aaaatggcca agataaagac
    22801 cacagcaggt gtcttataaa gaatacttaa aatctggctg acacaagaac caataatggc
    22861 tgcaatgaag gtagctacaa tgacattggt cggttccttg agcaagagat agattagcca
    22921 gacagtcatg cccaaaatcc ctccaggtaa gagcatagac cgttgcacat tgagtacgat
    22981 taaaaaagtg ataatggcaa gaaaacttgc tactgcttgt aataaaaagg ttgttagtgt
    23041 catattagtt catcaatacc aaggcgacag aagttcctgc ccctaaagcg agggtaatga
    23101 gcagggattc aaacatctta ctcataccag agtttatgtg gttggtcata atatcacgga
    23161 ccgcattggt caaggcaata cctggtacaa acggcatgac cgcaccagct ataatcaaat
    23221 ctgccgttga aggaaaacct gtgtagcgag cccaaaactg ggcaattatc ccaaagacaa
    23281 aggctccagc aaaggctgtc acaaagggaa ttcggataaa tttttccaca tagagggaaa
    23341 aggcaaaacc aaataaggtc gccactcctg ccccaagtgc gtcgtagata tttccgctaa
    23401 acataactga aaagaaagga gcactaaagg tcgcagccag agttacctgc aacttagtat
    23461 agggaagggg tttggcttgc aaggccgtca attgcttaaa ggctgtttct aagtcaatct
    23521 gccccccaac tagctgacga gaaatctggt tcacatcgca gactttttcg atgttataag
    23581 aagaggaggt cacgcgcttc atgcgcgaaa tattggtatt ttcaatagag aaaaagatag
    23641 cggcaggcat ggcaaggaca ttgcaatcca caatcccctg cgaatgcgcg attcgaatca
    23701 tggtatcttc tacacgatgg atttctgagc cacttttaag gagaatagtc cccgctagca
    23761 taatcacatc aatgacggca tttaattctt ttgattcttc catgctttcc tccttttatc
    23821 aactccctct attctatcac aaatccggac tcaaaaaaaa tctttgccat gaaatcatga
    23881 caaagattga ttactcattt tgattatcca tctgctttta aggagtagct gaagttgttt
    23941 taggtttgta gattgaaatc ttgactctag tcttatttag gtctaccttt tcacctgctc
    24001 taggactttg ttcaacaacc atgccttcta cactacctgc aggcgctgtc gtcacttcta
    24061 caacttctat attagcttcc ttaatcccaa caatttgaat caaattgttc ttagtaaact
    24121 ccaagctgga accaatgtaa ctcggcatgg caacacttgt aactttttta gctactgtca
    24181 agacaatttg agtaggttta ctcacatcat aagtcgttcc ggcacctgga ctttgtttca
    24241 taatcgttcc tggttcgctt tcgctggact cttcttcctc tatcttaatc aaattctcag
    24301 gaaccttctt ctgcttgagt tctgagatta cttctgtaga gttccgtcca atatagttcc
    24361 ctaattgaat cgtcgtagct tttttagcta ctgtcaaaac aatttgagtt gccttgctca
    24421 agtcataggt cgtaccttct ggtagacttt gcttcaagac cgttccagcc tcactctcat
    24481 tggactcttc ttcctcaatt ttaatcaaat tatctggaac ttttttctct tttaattccg
    24541 cgataacatc agaagatttc cggccgacat aattactaat ttggaaggat tgtttgcctg
    24601 atgagacaac cagattgatt ttcgttcctt cttttcgacc agttccagcg ccaggatctg
    24661 tacggataat ccgcccttct tccacctttt cactagcctc tgtcttctcc tcaccaatct
    24721 caaaattggc ttttttgagc gttgccttgg cctctgcaac tgtctgacct gccacatccg
    24781 gaatggcaat ggttgcagga gttctggata agatccaaat aagagaagct gccaccaata
    24841 caaggctggc caacaaaatc aggtaacgca tcttaaatct atgttttttc ggtgcttgtg
    24901 gttggtaagt ttcctctgtc acagcctggc ttgggttttt gattgatttg tgttctgtct
    24961 gcgcttgaac cttaggaata gatgtcaagg tactctgaga aaccttcggc aaggtcttgg
    25021 tatctgcctt gctcgtttca tcaaagatta acttactttc atttctacga ttgtaggaca
    25081 agctactaga caagtccaca tacatctctg aaaccgatcg gtagcgattg gtcaactttt
    25141 tagcagttgc cttgataata acattttcta aagcctgagg tacagatgga ttttctgcaa
    25201 taacggacgg caggggtttc tggaaatgct ggagggcaat ggtcaccgcg ctatccccgt
    25261 cataagggat atggcctgtc aacatctcat agaaaataat ccccatggca tagatatcac
    25321 tctgcacagt cgccttagaa ccacgcgcct gctctggtga caagtaatga actgagccca
    25381 acatcgagtt agtctgggtc agacttgtct ctgcaaaggc tacagcaatc ccaaagtctg
    25441 tgaccttggc agtcccatct ggtgtcaaaa ggatattttg aggtttcaag tccctgtgaa
    25501 caattcctcg agtatgggcc aagcgcatag ccaagagaat ttgtcccatg atacggacgg
    25561 cttcttcatt agaaagagga taatgttcct tgatatagcg tttgaggtct agtccagcaa
    25621 catactccat tgcaagatac tgttgaccgt cttcctcacc aatatctgtt atccgaacga
    25681 tatgaggatg gtctagatct gccatagctc tcgcttcacg ctgaaaacga gctacagcta
    25741 tcgggtccgt ctggtagttg gtcctcagaa ccttcactgc cacttcttcc ccatctaaga
    25801 ttaagtcttt ggctaggtaa acatccgcca tgcctcctcg accaatctgt ttgacaatcc
    25861 gatagcgtcc ggcaaaaatc ttgccgattt ggatcattct tcatcctcct cgttcataga
    25921 aacaagggca accgtaatgt tgtctaaacc tcctgcattg ttagcaaaac gaacaagtgt
    25981 ctccgtttta tctgctaaag gaatatcact ggttacaata tcacgaatct cactgcctga
    26041 aatcatgttg gtcaagccgt cactattgag caagagatag tcacctgact caaggataac
    26101 tgtcccaaaa tcaggctgaa tttcatcttt ttgcccaata gactgggtga taatattttt
    26161 ttgcggatga gcttctgcct cttctggtgt caattgacca gccttgagca attcattaac
    26221 caaggaatga tcgctcgtca actgatggta ttcttctcca cgaatcaagc cgatacgcga
    26281 atcaccaata tgagcataga tagcctgatt atcaataata gcaaggactt ccaaagtagt
    26341 tcccatgcct ctgtaagctt catcctgacc aagctggtga atcttttgat tttcaatttc
    26401 taggtaatgg gcgaaccatt cacgcacttc attgactgta tcgatctggg tatcaaccca
    26461 agctacaccc aggtctgtga ccgccatttc actagcgata ttccctgcac gatgacctcc
    26521 catcccatca gctaaaataa tcatggtacg tccagctcta ttgacatagt ggttgacata
    26581 gtcttggtta tttgttcgtt tctgaccaac atctgttaat aatgaaattt ccatgtgtca
    26641 gttccttcct aatccgatat cttgcgaaat tgactgatga agaatccatc acttccatac
    26701 aattcaggtg taatgaggat acagccgtct ttcatgatat ccttacattc atgttctagt
    26761 tttacctgct cgaactcggg atgactctct aaaaaggcct taacgacttg aaaattctcc
    26821 tctgagacga tagtgcaggt gctataagtt attataccac ctttgcctag tatttgacaa
    26881 acactaccta atatttctaa ctgaatttcc tgcaaggacg cgaaatctgc cgtttcttta
    26941 ttgtatttga tatctggttt tcggcgcaaa agaccgattc ctgaacaagg agcatccacc
    27001 aaaatcttat caaaggaatc ctggtcaaaa aactcatgca cctttctggc atccaatttt
    27061 tgagtttgaa cccgatcttc aactcccaga cgttgggcat tttcttgaat taaatccaac
    27121 ttgtggtcgt acaagtccag agcagtaacc tgacctgtcg taagataaga ggctatatgg
    27181 gctgttttcc cacctggagc cgcacaggca tcaagcactc gctcatcacc ttgtaaatca
    27241 agcgtcggag caaccagctg actggactcg tcttggatgg taatggctcc atccgcaaac
    27301 aaattatgcc ctgcaaaatg cccctgctcc ttaaccagac cagtggttgc taaaagggaa
    27361 tcattcgcct ccaacaaggc ttggatttcc tcttttcgac ttaggtctgt tacacgaata
    27421 ctggctttgt ttcgcactaa caggctttca aagatggctt ttgctctctc ctctccgtat
    27481 tcttccttga gtttggcaac tagccaaact gggagagaat aggcaatgga gtcacgcttg
    27541 ttttttcgct tgatgctagc aatatctggc cagccttcac gcaagatacg gcgaaggagg
    27601 gcattgacca atttttcact gcctttttta cgaagtttgg ccaattccac tgcttcattg
    27661 accacagcat gatctggaat cttgtccaaa tagcggagtt ggtaggcact catgagaaga
    27721 aggacataga gccagctgtc taactggtct ctgtcttcga taaagtggga taggtaccat
    27781 tccagagtca gtttacgggc taccgttcca tagaccagct cggtcactaa gcccttgtct
    27841 gctgccaaaa gttgactccc ctttagatgc ttatttaagg cgatatttga gtatgcttgg
    27901 ttcacaaaaa catcctctag cactgctaga gctaaacttc tagccgtttc tactttagtc
    27961 accaaatcgt tctcctacag tcaatgtacg tccaactccg ttgaggaagg aagcaatgtc
    28021 catcttaggc ttaccagctg gctgcacttg tttgagggat agagcccctt cagccgttgc
    28081 gacaatcaat tctttcttgc cgatagagag aatctcacct ggatttccct gaccttctac
    28141 tggtagggct tcataaatct taaagcggtc gcccttaagg aaagcatggg caacaggcca
    28201 ggggttcatt ccacgaattt ggttaaagag ttgacgattg gttttgttcc agtccagttt
    28261 ttcttcctct ggctttatat ttggagagaa ggtaacctga ctcgtatcct gcggttcagg
    28321 tttgatatca ccagcaatat aggcaggcag agtgtccaaa agcaaatcac gaccaactag
    28381 cgccaatttt tcaaacaagg tgccaacatt gtcctcatct gtgatcggaa tgctgcgacg
    28441 agaaatcata tctcctgcat ccatttcctt aaccatttcc atgatggtca caccagcttc
    28501 ctcatcccct tgaatcaagg cataatggat aggcgcacca ccacggtgtc taggaaggag
    28561 ggaggcatga acgttgacag caaagtccat gctatcaagg agtttgcttg ggagaaactg
    28621 cccaaaagca gcagtcacaa ttccatctgc tcctagcttc ataagttctt ccatctctgg
    28681 acttccagat aatttttcag gttggtagat agatagtcct gcttccttgg cagcctgctt
    28741 gactggggtt tcttggataa cttttttacg accaacagca cggtctggct gggtcacaac
    28801 ggctagaatt tcgtaacggt catctgtcaa aagtcctttt aagactgttg ctgaaaagtc
    28861 gggggtcccc ataaagatta gttttgtcat atcttctcct tcttataaaa attgctgcgg
    28921 ctcatggtca atgctgagac ggagctcact attttcccgt tcttgagtca aggccaagac
    28981 ctggttgagg gtcggcccca gctcatcttc taaacggtat ttaattaaaa tctggtaatg
    29041 atagaggttg tgggtacggg caatcggttt tggcgttggc cccagaatgg gactggtctc
    29101 tgacaagcct gaccgcaaaa tgttcatgac ttcataggca cgtttgaaaa cctcttcttc
    29161 tttcttgtga gaaagggtaa taccaatcgt gaaatagtaa ggtggatagc cgagttgtcg
    29221 tctgattccc atttcatagg cataaaagcc ttcgtaatct tgatccttgg caaatcgaat
    29281 agcatagtgc tgcgaattgt aggactgtat caagacttga cctgcttttt cagcccgacc
    29341 tgctcgacct gccacctgag tcaagagctg gaaggttctc tcagaagaac ggaaatcagg
    29401 cagattcaag gccgtatccg catttagaac tccgactagg gtaacattgg gaaaatccaa
    29461 accctttgca atcatctgag taccaagtaa aatatccgct tcccctcgcc caaactggtc
    29521 aagcaaggct tggtgactgc ctttctttcg agtcgtatcc acatccatcc tcaaaatgcg
    29581 agcttgggga aagagttctg ctagctcatc ataagccttc tgagttcccg tcccatagta
    29641 acgaatactg cggctcttac agttaggaca gacctgagga atatccttcg agaaaccaca
    29701 ataatggcag ttcatagtct tggtatccat atgcaaggtc agagaaatat cgcagttggg
    29761 acaagtatcc accgtcccac actcccgaca catgacaaag ctagaataac cacggcgatt
    29821 gagcatgaga accacctgct cttttttaac cagacggtct tggatagcct ctagcaaagg
    29881 aggcgtaaag tttgacgtct cattttgtcc gatatagtct cgaaagtcaa tcacttgaac
    29941 ctcagggatt gtagccaaag gattagcacg ttgggttaga cgtaagtgtt gatagacgcc
    30001 tttgccagca cgtgcccggc tctctaagct cggcgttgca gatccaagta ccagagttgc
    30061 ttgattatac tgagcccgta aaatagctac atctctggca tggtaacggg gattgctgtc
    30121 ctgcttataa gccacttcat gctcttcatc aataatcatg acacccagat ttttcagagg
    30181 agcaaagata gcagatctgg caccaacaac aacttgggca tcgccacgct ccaccttgcg
    30241 ccattcatca tacttttcac cattggataa tcctgagtga agaatggcta ccttgtcccc
    30301 aaaacgtgct ataaaacgct cggtcatctg aggagtcaag gaaatctcag gtaccagcaa
    30361 aatagctgtc ttgcccttat ccagggcacc ttggataatc tgcaagtaaa cctcggtctt
    30421 cccacttcct gtaatccctt gaagtagaaa gggaggttga gaactgccaa tagaactcac
    30481 aaccgcatca cgcgcctgtc tttgttctgg atttaactcc aaaggtctac ttgcttcaat
    30541 tccttcaaaa taagcagccg agcgttgaac ttccttttgg actatggtaa cagcaccttg
    30601 atccacaaag aagttgactt gctctcgcga gtaggactct aacaagctag ccaaggaagc
    30661 gctctctgga tgagacagca gataatctct cagttccaac tttttcttgg cacgtgtaga
    30721 aatctcaaca ccttctaatt gagcatggtc aacctcatac caagactggg tcttgacctt
    30781 cttttgatcg actgcctgat attccagacc aagcaggcct tttctagtca aacgcatcat
    30841 ttcagcttgc ttggcaaggt ctagtgaaga aaaggctagc gaatcttctg aaccaaacag
    30901 gcgcactcgt tcttcctgac tcaagccttc cagaggatag agaatcttgt catagctaga
    30961 attcagaaat cctggaagca tggccttgag gatagaaatt ttgtaggaga agacagattt
    31021 gcgtaactcc tcagccagcc agagttgttc tggcgtgaga acaggagaaa aatccagcac
    31081 ctctgcaata tcttttaaat cttgctccat ctcttctcca tctgattggg actccaaacc
    31141 aagaacaatc ccttgaatca ggcgattccc cttaccaaaa ggcacatgaa cccgcatccc
    31201 aacttccagc attccctcaa attcctccgg aatcctgtaa ctatagggct ggtccgtctg
    31261 catcaagggc acatctacga taatcttagc tagggccatc gtctcacctc ctccttgtca
    31321 gtacattctt gcaatagaaa aaataagatt gagtcccccc aaccttaaat tttttcacca
    31381 tcttcttttt ctttagcaat ttgctctttg attttctttt cttcttcttc tttgcggcgt
    31441 ttttcttctt cgatacggcg acgcactgct tcacgttttc cttctggatc tgggtgaatt
    31501 gtaacgtttc ctgattcgat ttcttctaaa gcgcgaagag ttgatttttc agacttaaaa
    31561 ccttgagttg ctggggcacc tgcttccaat tcgtgggcac gttttgcttc caagattacg
    31621 agtgaatatt ttgaaggaac cttgtcgagc aaggtatcaa tagagggttt taacatcatt
    31681 tgcttgtacc tattttctaa attttatcgg gtagttggag attttggtaa catctcctga
    31741 tagtgaccaa tgacacgatc cacacagaag tgttctgctt caatcacaca tttgacacgt
    31801 tcagcagcta ggggtacctg atcgttgaca atcgcataat catactcacg catgagggca
    31861 atttcttcct tggccttttc gattcgttgg gcaatcactt ctgcactatc tgttccacga
    31921 cctaccaagc gatcttgcaa ttcatccaaa tctggtggtg tcaggaagat aaagacagca
    31981 tctggaacct ttttcttgac ctgaagagca ccctgaactt caatttcaag gaaaacatcg
    32041 attcccttgt ccaaggtttc attgacatag gtcagaggag ttccatagta gttgccgaca
    32101 tattctgcgt attccaacat ctgtccttga cgaatcagct cttcaaattc ttcacgagta
    32161 cggaagaaat agtcaacacc gtccacttct ccaggacgtt gtgcgcgtgt cgtcatcgat
    32221 acagaatatt gaaattggtt ttcagaactc tcaaaaatct ctcttctaac cgttcctttt
    32281 ccaacccctg aaggaccaga aaaaacgatt agtaagcctc ggtctgccat tgtgtctcct
    32341 tttagtcaat ctgtgaaata acatttctct agaataatgg caaaaagcca gattatcctt
    32401 tacagtcttt ctatctagtg taacaaaaaa gcagtaattt ttcaactgct ctttcttatt
    32461 tatttagcat aatctactgc acgaagctcg cgaatcacgg ttaccttgat atttcctgga
    32521 taatcgagat tgttttcaat tttcttacga actttgtgag ccaagattgt gactttgtcg
    32581 tccttgattt ttcctggatt gaccatgata cgaatttcac gtcctgcttg aagggcaaag
    32641 ctagtttgca ctccttcaaa gccgttagca atttcttcca aatcatggag acgcttgatg
    32701 tagctttcaa gagactcact acgagcacct ggacgggctg cgctcaaggc atctgctgca
    32761 gcgacgataa ctgctatcac gctctcagct tcaacatctc cgtggtgact agcaatcgta
    32821 ttcaccacaa ctgggggttc cttgtactta cgggccaatt ccataccgat ttcaacgtgg
    32881 ctaccttcaa cctcatggtc aatggctttc ccgatatcgt gaaggaatcc agcacgacgg
    32941 gcaagagccg cattttcacc aagttcgctc gccatgatac cagccaactt agcaacctca
    33001 atcgaatggc gcaaaacatt ttgtccatat gaagtacgga actgcaaacg tcccataatc
    33061 ttcatcaagt ctggatgaag gtttggcgca ccaatttcat aggcagcagc ctcaccgtat
    33121 tcacgaatct tattgtcaat ctcttgacgg tttttctcaa ccaactcttc gatacgagct
    33181 ggatgtatac gaccatcttt gagcaacatt tccatagtca tacgggcaat ctcacgacga
    33241 atcggatcaa atcctgacaa ggtcaccact tctggtgtat cgtcgataat cacatcgacc
    33301 cctgtcaaac tttcaaaggt acgaatgtta cgaccttcac gaccaataat gcgtcccttc
    33361 atagtatcgt ctggcagatg aactgttgag tttgttgact ccgctacata ttcaccagcg
    33421 atacgttgca tagcttgaac caagatgtcc ttggccattt tgtcagaacg ttccttgacc
    33481 tcttgctcag cttcgcgaat gcgactggca atctccctgg tcaagttttc ctctgtctga
    33541 gccaagataa tatctcgtgc ttctgcctga gacagagcac caatacgctc tagttctgct
    33601 tctttttgtc tttcgacttc ctctaattgc tcttcacgcg catcaaggtt tttcgctcta
    33661 tcagaaatac tttgttcttt ttgttcaagt gtttgttctt tactcgtcaa attgtcgtcc
    33721 ttacggtcaa ggctagtagc tctctctgtc aaacgacttt cgatttgttt gagttcttga
    33781 cgttctgatt tgaattcagc gtccacttct tcacggtatt ttctggcttc ttctttggcc
    33841 tccaatagtg cttctttttt aagagacttg ctttcacgtt tggcttcatt aacaagtaaa
    33901 tccgcttcac gctcagcttg tccacgtaaa ttagttgctt cttgttcagc atttaaaagc
    33961 atcaactctg cagcttcctg agatgatttc atcttagctg agatgctgac atatccaatg
    34021 actaaaccaa tgatgacggc aaaaacagca atcgcaagcg acatgatttc catgttttta
    34081 cctcatttta ttgttattcc gaatgacata cattctttta cattctacca taaaaaaaga
    34141 atttaaacaa accttaaatg gatatcgcta acatccgcat tatcactatc atagttatca
    34201 tttacagata taaagcgaat acctttttca ggaaatatct tttgtaagta ctttcctgac
    34261 tcgatataat ctctgccgaa acgagataaa tcttttacca taatgataga aaactttcct
    34321 ttattcaggt catcaatcat attcttaaaa tttggtcgct caaaattaga accactatat
    34381 ccatcatcta catattcata ggataattca atagatttct cttctacctc caaaaagcaa
    34441 aaaggataaa tttcccctac actttaatag tgaaaattta tccctcttgg taagctcttt
    34501 tattaatttt tttgataaag aaaacgcaca t
//
LOCUS       DAVKFH010000019        34400 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKFH010000019 DAVKFH010000000
VERSION     DAVKFH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.019"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            559..1011
                     /locus_tag="VW006_000964"
     CDS             559..1011
                     /locus_tag="VW006_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9379836.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1030..2367
                     /locus_tag="VW006_000965"
     CDS             1030..2367
                     /locus_tag="VW006_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9379837.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2446..3330
                     /locus_tag="VW006_000966"
     CDS             2446..3330
                     /locus_tag="VW006_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9379838.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWIINGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3330..4163
                     /locus_tag="VW006_000967"
     CDS             3330..4163
                     /locus_tag="VW006_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9379839.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4181..6274
                     /gene="nanB"
                     /locus_tag="VW006_000968"
     CDS             4181..6274
                     /gene="nanB"
                     /locus_tag="VW006_000968"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9379840.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6286..7389
                     /locus_tag="VW006_000969"
     CDS             6286..7389
                     /locus_tag="VW006_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9379841.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7563..8261
                     /locus_tag="VW006_000970"
     CDS             7563..8261
                     /locus_tag="VW006_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9379842.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8280..9797
                     /locus_tag="VW006_000971"
     CDS             8280..9797
                     /locus_tag="VW006_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9379843.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9922..11250
                     /locus_tag="VW006_000972"
     CDS             9922..11250
                     /locus_tag="VW006_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9379844.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11502..12368
                     /locus_tag="VW006_000973"
     CDS             11502..12368
                     /locus_tag="VW006_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9379845.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAIMIAIVSF
                     VQFKVLGNDVEY"
     gene            12384..13223
                     /locus_tag="VW006_000974"
     CDS             12384..13223
                     /locus_tag="VW006_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9379846.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13495..13947
                     /locus_tag="VW006_000975"
     CDS             13495..13947
                     /locus_tag="VW006_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9379847.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14044..14388
                     /locus_tag="VW006_000976"
     CDS             14044..14388
                     /locus_tag="VW006_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379848.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14675..15319
                     /locus_tag="VW006_000977"
     CDS             14675..15319
                     /locus_tag="VW006_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9379849.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15488..16405
                     /locus_tag="VW006_000978"
     CDS             15488..16405
                     /locus_tag="VW006_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9379850.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEDDRPVVEAAAALIRETKERFL"
     gene            16423..17307
                     /locus_tag="VW006_000979"
     CDS             16423..17307
                     /locus_tag="VW006_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9379851.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17323..18174)
                     /locus_tag="VW006_000980"
     CDS             complement(17323..18174)
                     /locus_tag="VW006_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9379852.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18374..20431
                     /gene="pbp2b"
                     /locus_tag="VW006_000981"
     CDS             18374..20431
                     /gene="pbp2b"
                     /locus_tag="VW006_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9379853.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20442..21038
                     /gene="recR"
                     /locus_tag="VW006_000982"
     CDS             20442..21038
                     /gene="recR"
                     /locus_tag="VW006_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9379854.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21214..22257
                     /locus_tag="VW006_000983"
     CDS             21214..22257
                     /locus_tag="VW006_000983"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9379855.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22341..23714
                     /locus_tag="VW006_000984"
     CDS             22341..23714
                     /locus_tag="VW006_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9379856.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            23701..24312
                     /locus_tag="VW006_000985"
     CDS             23701..24312
                     /locus_tag="VW006_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9379857.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24403..25107
                     /locus_tag="VW006_000986"
     CDS             24403..25107
                     /locus_tag="VW006_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9379858.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25326..26699
                     /gene="ftsA"
                     /locus_tag="VW006_000987"
     CDS             25326..26699
                     /gene="ftsA"
                     /locus_tag="VW006_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9379859.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26716..27975
                     /gene="ftsZ"
                     /locus_tag="VW006_000988"
     CDS             26716..27975
                     /gene="ftsZ"
                     /locus_tag="VW006_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9379860.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27980..28651
                     /locus_tag="VW006_000989"
     CDS             27980..28651
                     /locus_tag="VW006_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9379861.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28661..29200
                     /locus_tag="VW006_000990"
     CDS             28661..29200
                     /locus_tag="VW006_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9379862.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29200..29463
                     /locus_tag="VW006_000991"
     CDS             29200..29463
                     /locus_tag="VW006_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9379863.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29460..30245
                     /locus_tag="VW006_000992"
     CDS             29460..30245
                     /locus_tag="VW006_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9379864.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30254..31042
                     /gene="divIVA"
                     /locus_tag="VW006_000993"
     CDS             30254..31042
                     /gene="divIVA"
                     /locus_tag="VW006_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9379865.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31390..34182
                     /locus_tag="VW006_000994"
     CDS             31390..34182
                     /locus_tag="VW006_000994"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9379866.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10583 a         6071 c         7485 g        10261 t
ORIGIN      
        1 acaaaactaa tttagagaat gaaatataga gtatttctct cttaaaagtt tttggtgaaa
       61 cgagatgtag aaaggagatt tagccaaaga gtctattagt gctagaataa tagattagaa
      121 ttattttaga aaaacgaagt gagcagctta taaattcaag tccccaaata gattcatact
      181 agtatctttt gcaaaaaata aagggcgact tccttcatga atatcaattt catctataag
      241 gaaggtagct aattgaacta acttatttat tctgtttgtc gctagaaaaa tcagacctcc
      301 ttgtgaagat tgaggagata cttaatgaaa atcaaagaag aaactagcaa gctagtagca
      361 gattgcccaa aacaccgctt tgaggttgta gataagactg acgtatataa tccaaggtga
      421 agcgactgtg gtttgaagag attttcaaag agtataggct agagagtagt gtttttatgt
      481 ccttctagta gaaaatgcta gacagaagaa tggggaactt ggataggaga aatagattga
      541 gaaaggaggt tagaagagat gattattaca aaaattagcc gtttaggaac ttatgtggga
      601 gtaaatccac attttgcaac attaatagat tttctagaaa aaacaggact agaaaattta
      661 acagaaggtt cgattgctat cgatggtaat cgattgtttg ggaattgctt tacttatcta
      721 gcagatggtc aagcaggggc tttctttgaa acacaccaaa aatatttgga tattcattta
      781 gttttggaaa acgaagaagc catggctgtt acatcgccgg aaaatgtaag cgttacccaa
      841 gaatatgatg aagagaaaga tattgaatta tacacaggga aagtggaaca gttggttcag
      901 ttgagagctg gcgaatgcct catcactttt ccagaagatt tacatcaacc caaggttcgt
      961 ataaatgatg aacctgtgaa aaaagttgtc tttaaagttg cgatttctta atgtagaaag
     1021 agaagaacga tgaaaaaaat gagaaagttt ttatgtctag ctggaattgc gctagcggct
     1081 gttgccttgg tagcttgttc aggaaaaaaa gaagctacaa ctagtactga accaccaaca
     1141 gaattatctg gtgagattac aatgtggcac tcctttactc aaggaccccg tttagaaagt
     1201 attcaaaaat cagcagatgc tttcatgcaa aagcatccaa aaacgaaaat caagattgaa
     1261 acattttctt ggaatgactt ctatactaaa tggactacag gtttagcaaa tggaaatgtg
     1321 ccagatatca gtacagctct tcctaaccaa gtaatggaaa tggtcaactc agatgctttg
     1381 gttccgctaa atgattctat caagcgtatt ggacaagata aatttaacga aactgcctta
     1441 aatgaagcaa aaatcggaga tgattactac tctgttcctc tttattcaca tgcacaagtc
     1501 atgtgggtta gaacagattt gttaaaagaa cataatattg aggttcctaa aacttgggat
     1561 caactctatg aagcttctaa aaaattgaaa gaagctggag tttatggctt gtctgttccg
     1621 tttggaacaa atgacttaat ggcaacacgt ttcttgaact tctacgtacg tagtggtgga
     1681 ggaagcctct taacaaaaga tcttaaagca gacttgacaa gccaacttgc tcaagatggt
     1741 attaaatact gggttaaatt gtataaagaa atctcacctc aagattcttt gaactttaat
     1801 gtccttcaac aagctacctt gttctatcaa ggaaaaacag catttgactt taactctggc
     1861 ttccatatcg gaggaattaa tgccaacagt cctcaattga ttgattcgat tgatgcttat
     1921 cctattccaa aaatcaaaga gtctgataaa gaccaaggaa ttgaaacctc aaacattcca
     1981 atggttgttt ggaaaaattc aaaacatcca gaagttgcta aagcattctt agaagcactt
     2041 tataatgaag aagactacgt taaattcctt gattcaactc cagtaggtat gttgccaact
     2101 attaagggga ttagcgattc tgcagcctat aaagaaaatg aaactcgtaa gaaatttaaa
     2161 catgctgaag aagtaattac tgaagctgtt aaaaaaggta ctgctattgg ttatgaaaat
     2221 ggaccaagtg tacaagctgg tatgttgact aaccaacaca ttattgaaca aatgttccaa
     2281 gatatcatta caaatggaac agatcctatg aaagcagcaa aagaagcaga aaaacaatta
     2341 aacgatttat ttgaggctgt tcagtagatg taaaagacta gaaaataggt gggctagtga
     2401 gctgaaaagc tctagcccaa tcttgtaaaa gaagggagaa ggagaatggt taaagaacgt
     2461 aatttaactc gctggatatt tgttttgcca gctatgatta tcgtaggatt actctttgtt
     2521 tatccgtttt tctcgagtat tttttatagc tttaccaata agcatttgat tatgcctaat
     2581 tataaatttg ttggtttggc taactataaa gctgtgctat cagatcccaa cttctttaat
     2641 gcgttcttta attcaattaa gtggaccgtt ttctcattag ttggtcaagt tttagtaggg
     2701 tttgtattgg ctttagctct tcacagagta cgccacttca agaaattata taggacctta
     2761 ttgattgttc cttgggcatt tcctaccatc gttattgcct tctcttggca gtggattata
     2821 aacggggttt atggctactt acctaatcta atcgtaaaat taggtttaat ggaacataca
     2881 cctgcatttt tgacagatag tacatgggca ttcctatgtt tggtgtttat caacatttgg
     2941 tttggagcac caatgattat ggttaatgtg ctttcagctt tgcaaacagt accagaagaa
     3001 caatttgagg ctgctaagat agatggtgct tcaagttggc aggtgttcaa gtttatcgtc
     3061 tttccacata ttaaagtggt tgtaggactt ctagttgttt tgagaactgt atggatcttt
     3121 aataactttg acattatcta cctcattact ggtggtggac cagccaatgc tacaacgacg
     3181 cttccaattt ttgcttacaa cctgggttgg ggaactaaat tgttgggtcg tgcttcagca
     3241 gttacagtat tgctctttat cttcttggtg gcgatttgct ttatctactt tgctatcatc
     3301 agtaagtggg aaaaggaggg tagaaaataa tgaagaagaa atccagtatt tatttagata
     3361 ttctctcaca tgtactttta gttggtgcga ccatcgttgc aattttccca ttggtatgga
     3421 ttatcatatc ttctgtcaaa gggaaagggg aattaactca gtatccaaca cgattttggc
     3481 ctgaacagtt tacattagat tatttcactc atgttatcaa cgatttgcac ttcattgata
     3541 acattcgaaa cagtttaatc attgccttgg ctacaaccct cattgcgatt attatttctg
     3601 ctatggcagc ctatggtatt gttcgattct ttcctaaatt gggagcaatc atgtcgagac
     3661 tactcgtcat tacctacatt ttcccaccaa ttttgttagc aattccctat tcaattgcca
     3721 ttgctaaagt tgggttaaca aatagtttat ttggcttgat gatggtttat ctatctttta
     3781 gtgttccata tgcagtttgg ctcttagttg gatttttcca aacagttcca attggaattg
     3841 aagaagcggc tagaattgat ggtgcaaata aatttgttac gttttataaa gttgtgctac
     3901 cgattgtagc accaggtatt gtagcaacag ctatttatac atttatcaat gcttggaatg
     3961 aattcctgta tgccttgatt ttgattaaca atacaggaaa gatgacagta gcagtagccc
     4021 ttcgttcact taatggttca gaaatactag actggggaga tatgatggca gcgtctgtta
     4081 ttgtagttct tccatcaatt attttcttct ctatcatcca aaataagatt gcaagtggat
     4141 tatcagaagg atctgtgaag tagacgaaag aaggaaaaaa atgaataaaa gaggtcttta
     4201 ttcaaaacta ggaatttctg ttgtaggcat tagtctttta atgggagtcc ccactttgat
     4261 tcatgcgaat gaattaaact atggtcaact gtccatatct cctatttttc aaggaggctc
     4321 atatcaactg aacaataaga gtatagatat cagccctttg ttattagata aattgtctgg
     4381 agagagtcag acagtagtaa tgaaatttaa agcagatgaa ccaaactctc ttcaagcttt
     4441 gtttggccta tctaatagta aagcaggctt taaaaataat tacttttcaa ttttcatgag
     4501 agattctggt gagataggtg tagaaataag agacgcccaa aagggaataa attatttatt
     4561 ttccagacca gcttcattat ggggaaaaca taaaggacag gcagttgaaa atacactagt
     4621 atttgtatct gattctaaag ataaaacata cacaatgtat gttaatggaa tagaagtgtt
     4681 ctctgaaaca gttgatacat ttttgccaat ttcaaatata aatggtatag ataaggcaac
     4741 actaggagct gttaatcgtg aaggtaagga acattacctc gcaaaaggaa gtattggtga
     4801 aatcagtcta tttaacaaag caattagtga tcaggaagtt tcaactattc ccttatcaaa
     4861 tccatttcag ttaattttcc aatcaggaga ttctactcaa gctaactatt ttagaatacc
     4921 gacactatat acattaagta gtggaagagt tctatcaagt attgatgcac gttatggtgg
     4981 gactcatgat tctaaaagta agattaatat tgccacttct tatagtgatg ataatgggaa
     5041 aacgtggagt gagccaattt ttgctatgaa gtttaatgac tatgaggagc agttagttta
     5101 ctggccacga gataataaat taaagaatag tcaaattagt ggaagtgctt cattcataga
     5161 ttcatccatt gttgaagata aaaaatctgg gaaaacgata ttactagctg atgttatgcc
     5221 tgcgggtatt ggaaataata atgcaaataa agccgactca ggttttaaag aaataaatgg
     5281 tcattattat ttaaaactaa agaagaatgg agataacgat ttccgttata cagttagaga
     5341 aaatggtgtc gtttatgatg aaacaactaa taaacctaca aattatacta taaatgataa
     5401 gtatgaagtt ttggagggag gaaagtcttt aacagtcgaa caatattcgg ttgattttga
     5461 tagtggctct ttaagagaaa aacataatgg aaaacaggtc cctatgaatg ttttctacaa
     5521 agattcgtta tttaaagtga ctcctactaa ttatatagca atgacaacta gtcagaatag
     5581 aggagagagt tgggaacaat ttaagttgtt gcctccgttc ttaggagaaa aacataatgg
     5641 aacttactta tgtcccggac aaggtttagc attaaaatca agtaacagat tgatttttgc
     5701 aacatatact agtggagaac taacctatct catttctgat gatagtggtc aaacatggaa
     5761 gaaatcctca gcttcaattc cgtttaaaaa tgcaacagca gaagcacaaa tggttgaact
     5821 gagagatggt gtgattagaa cattctttag aaccactaca ggtaagatag cttatatgac
     5881 tagtagagat tctggagaaa catggtcgga agtttcgtat attgatggaa tccaacaaac
     5941 ttcatatggc acacaagtat ctgcaattaa atactctcaa ttaattgatg gaaaagaagc
     6001 agtcattttg agtacaccaa attctagaag tggccgtaag ggaggtcaat tagttgtcgg
     6061 tttagtcaat aaagaagatg atagtattga ttggaaatac cactatgata ttgatttgcc
     6121 ttcgtatggt tatgcctatt ctgcgattac agaattgcca aatcatcaca taggtgtact
     6181 gtttgaaaaa tatgattcgt ggtcgagaaa tgaattgcat ttaagcaatg tagttcagta
     6241 tatagatttg gaaattaatg atttaacaaa ataaaggaga aaaacatggt taaatacggt
     6301 gttgttggag cagggtattt tggagctgaa ttggctcgct acatgcaaaa gaatgatgga
     6361 gcagagatta ctcttctcta tgatccagat aatgcagagg cgattgcaga agaattggga
     6421 gcaaaagtag caagttcctt agatgagttg gtttctagcg atgaagtaga ttgtgttatc
     6481 gtcgcaactc caaataatct tcataaggaa ccggttatta aggctgcaca gcatggtaaa
     6541 aatgttttct gtgaaaaacc aattgcgctt tcttatcaag attgtcgcga gatggtagat
     6601 gcgtgtaaag aaaacaatgt aacctttatg gcaggacata ttatgaattt ctttaatggt
     6661 gttcatcatg caaaagaact cattaatcaa ggagttatcg gagacgttct atattgtcat
     6721 acagctcgta atggttggga agaacaacaa ccgtcagtat catggaaaaa aattcgtgaa
     6781 aaatcaggtg gtcacttgta tcaccacatc catgaattgg attgcgttca attccttatg
     6841 gggggcatgc ctgaaactgt aaccatgaca ggtggaaatg tggcccatga aggtgaacat
     6901 ttcggtgatg aagatgatat gatttttgtc aatatggaat tttctaataa gcgttttgct
     6961 ttgttagaat ggggttcagc ttatcgttgg ggtgaacatt atgtcttaat ccaaggaagc
     7021 aaaggtgcca tccgcttaga cttattcaac tgtaaaggaa ctcttaagct agatgggcaa
     7081 gaaagctatt tcttgattca cgaatcgcaa gaagaagatg atgatcggac tcgtatctat
     7141 catagtacag agatggatgg agcaattgct tatggtaaac caggtaaacg tactccatta
     7201 tggctatcat ctgtcattga taaagaaatg cgctatctgc atgagattat ggaaggagct
     7261 ccagtatcag aagaatttgc aaaacttttg acaggtgaag ctgccctaga agcaattgct
     7321 actgcagatg cttgtaccca gtctatgttt gaagatcgca aagtaaaatt gtcagaaatt
     7381 gtaaaataaa ttttggtatt ctcctattta taggtcgact tgctcctctg aaagtacttt
     7441 tagaggagct gtttgacttt gctagttttt gaaactgaaa tctattatac tacaaactat
     7501 tgaaagcgtt ttaattttaa ggtataataa tctcatagaa ataaagaaaa ggaggaaaga
     7561 ggatgccaca gattagcaaa gaagccttga ttgagcaaat caaagatgga atcatcgttt
     7621 cttgtcaggc tcttcctcat gaaccgcttt atacagaagc gggaggggtg attcccttgc
     7681 tggtcaaagc ggctgagcaa ggtggagcag tcggtatccg agcaaacagt gttcgcgata
     7741 tcaaggaaat taaggaggtc actaaacttc caatcattgg gattatcaaa cgtgattatc
     7801 cacctcagga acccttcatc acggctacta tgaaagaagt tgatgaattg gcagaactgg
     7861 acatcgaggt gattgctctg gattgtacca agcgtgaacg ctacgatggt ttggaaattc
     7921 aagagttcat tcgtcaggtt aaggagaaat atcctaatca gcttttgatg gctgatacta
     7981 gtatcttcga agaagggcta gcagctgtag aagcaggaat tgactttgtc ggaacaacct
     8041 tatcaggcta cacatcctac agtccaaaag tagacggtcc agattttgaa ttgattaaga
     8101 aactctgtga tgccggtgta gatgtcattg cagaaggaaa aattcataca ccagaacaag
     8161 ccaaacaaat ccttgaatat ggagtgcgag gcatcgttgt tggtggcgcc attactagac
     8221 caaaagagat tacagaacgc ttcgttgcta gtcttaaata agatgtgagg gggagtttta
     8281 tgtttaaagt tttacaaaaa gttggaaaag cttttatgtt acctatagct atacttcctg
     8341 cagcaggtct acttttgggg attggtggtg cactttcaaa cccaaccacg atagcaactt
     8401 atccaatact agacaatagt atttttcaat caatattcca agtaatgagc tctgcaggag
     8461 aggttgtatt cagtaatttg tcactacttc tctgtgtggg attatgtatt ggcttagcga
     8521 aacgagataa aggaaccgct gcgttagcag gagtaactgg ttacttagtt atgactgcaa
     8581 cgatcaaagc tttggtaaaa ctttttatgg cagaaggatc tgcaattgat actggagtta
     8641 ttggagcatt agctgtcgga atagttgccg tatatttgca caaccgatat aacaatattc
     8701 aattaccttc cgctttagga ttctttggag gttcacgctt cgttcctatt gttacatcgt
     8761 tctcttctat cttgattggc tttgtcttct ttgttatttg gccacctttc caacaacttc
     8821 ttgtttctac aggtggatat atttctcagg cgggtccaat tggaactttt ctatatggat
     8881 ttttaatgag actttctgga gcagtaggct tacatcatat aatttaccct atgttttggt
     8941 atactgaact tggtggtgtt gaaactattg caggacaaac agtggttgga gctcaaaaaa
     9001 tattttttgc tcaattagcc gatccggccc attctggatt atttacagaa ggaacaaggt
     9061 tttttgcagg tcgtttctca acaatgatgt tcggtttacc ggctgcctgt ttagcgatgt
     9121 accatagtgt tcctaaaaat cgtcgtaaaa aatacgcggg tttgtttttt ggagttgctt
     9181 taacatcttt tattaccggt attacagaac caattgaatt tatgtttcta ttcgtcagtc
     9241 cggttctata tgttgttcac gcattccttg atggtgttag cttctttatt gcagacgtct
     9301 taaatatttc aataggaaac acattttcag gaggtgtaat cgatttcact ttatttggaa
     9361 ttttgcaggg gaacgctaag acgaattggg ttcttcagat tccaattgga cttatttgga
     9421 gtgttttgta ttatattatt tttagatggt tcattactca attcaatgtt ctaacgccag
     9481 gacgaggaga agaagtagat tctaaagaaa tttctgaatc cgcagattca acttcaaata
     9541 ctgcagatta tttaaaacag gatagcctac aaattatcag agccttgggt ggatcaaata
     9601 atatagaaga tgtagatgct tgtgtgacac gtttacgtgt agctgtaaaa gaagttaatc
     9661 aagttgataa agcactttta aaacaaattg gtgcagttga tgtcttagaa gtgaagggtg
     9721 gcattcaagc aatctatgga gcaaaagcaa tcttatataa aaatagtatt aatgaaattt
     9781 taggtgtaga tgattaagta cttactgact taataaaaaa cagaggagag tgatggatga
     9841 gtaggatgaa atgaaatcgc atacaagaaa taaagaactc attatccaag ttggatacgc
     9901 ttattacata ggagaataca aatgaaattt agaaaattag cttgtacagt acttgcgggt
     9961 gctgcggttc ttggtcttgc tgcttgtggc aattctggcg gaagtaaaga tgctgccaaa
    10021 tcaggtggtg acggtgccaa aacagaaatc acttggtggg cattcccagt atttacccaa
    10081 gaaaaaactg gtgacggtgt tggaacttat gaaaaatcaa tcatcgaagc gtttgaaaaa
    10141 gcaaacccag atataaaagt gaaattggaa accatcgact tcaagtcagg tcctgaaaaa
    10201 atcacaacag ccatcgaagc aggaacagct ccagacgtac tctttgatgc accaggacgt
    10261 atcatccaat acggtaaaaa cggtaaattg gctgagttga atgacctctt cacagatgaa
    10321 tttgttaaag atgtcaacaa tgaaaacatc gtacaagcaa gtaaagctgg agacaaggct
    10381 tatatgtatc cgattagttc tgccccattc tacatggcaa tgaacaagaa aatgttagaa
    10441 gatgctggag tagcaaacct tgtaaaagaa ggttggacaa ctgatgattt tgaaaaagta
    10501 ttgaaagcac ttaaagacaa gggttacaca ccaggttcat tgttcagttc tggtcaaggg
    10561 ggagaccaag gaacacgtgc ctttatctct aacctttata gcggttctgt aacagatgaa
    10621 aaagttagca aatatacaac tgatgatcct aaattcgtca aaggtcttga aaaagcaact
    10681 agctggatta aagacaattt gatcaataat ggttcacaat ttgacggtgg ggcagatatc
    10741 caaaactttg ccaatggtca aacatcttac acaatccttt gggcaccagc tcaaaatggt
    10801 atccaagcta aacttttaga agcaagtaag gtagaagtgg tagaagtacc attcccatca
    10861 gacgaaggta agccagctct tgaatacctt gtaaacgggt ttgcagtatt caacaataaa
    10921 gacgacaaga aagtcgctgc atctaagaaa ttcatccagt ttatcgcaga tgacaaggag
    10981 tggggaccta aagacgtagt tcgtacaggt gctttcccag tccgtacttc atttggaaaa
    11041 ctttatgaag acaaacgcat ggaaacaatc agcggctgga ctcaatacta ctcaccatac
    11101 tacaacacta ttgatggatt tgctgaaatg agaacacttt ggttcccaat gttgcaatct
    11161 gtatcaaatg gtgacgaaaa accagcagat gctttgaaag ccttcactga aaaagcgaac
    11221 gaaacaatca aaaaagctat gaaacaatag tccttagtta ttctataaaa agtagttttt
    11281 taaagaacct aagagtgtat accccctttt ccctctacac agatagtgta agaaaagggg
    11341 gcttttgttt aaaatgtaag aaactgtcac gaaattaaaa tgaagttctt acataagcga
    11401 atcataaaaa atttcatttt gattttaaaa cagttcaaga aagtcaaaaa attattctat
    11461 ttgaaagaga ggtgccgact gtgaaagtca ataaaatccg tatgcgggaa acagtgattt
    11521 cctacgcttt cctagcacca gtattattct tctttgtcat ctttgtgttg gctccgatgg
    11581 tgatgggctt cattacaagt ttctttaact actcaatgac taaatttgag tttgtaggct
    11641 tggataacta tatccgtatg tttaaagatc ctgtctttac aaaatctctg attaacacag
    11701 ttattttggt tattggatct gtaccagttg ttgttctatt ctcactcttt gtagcatctc
    11761 agacctatca tcaaaatgtc attgccagat ccttctaccg tttcgtcttc ttccttcctg
    11821 ttgtaacggg tagtgttgcc gtgacagttg tttggaaatg gatttatgac ccactatcag
    11881 ggattctaaa ctttgtcctt aagtccagcc acatcatcag ccaaaacatt tcttggttgg
    11941 gagataaaaa ctgggcattg atggcgatta tgattattct cttgaccact tcagttggtc
    12001 agcccatcat cctttatatc gctgccatgg ggaatattga caattcactg gttgaagcgg
    12061 cgcgtgttga tggtgcaact gagtttcaag ttttttggaa gattaaatgg ccaagccttc
    12121 ttccaacaac tctttatatt gcaatcatca caacaattaa ctcattccag tgttttgcct
    12181 tgattcagct tttgacatct ggtggtccaa actactcaac aagtaccttg atgtactacc
    12241 tttacgaaaa agccttccaa ttgacagaat acggctatgc caacacaatt ggtgtcttct
    12301 tggcaatcat gattgctatc gtaagctttg ttcaatttaa agtacttgga aacgacgtag
    12361 aatactaaag aaaggagaca gctatgcaat ctacagaaaa aaaaccatta acagccttta
    12421 ctgttatttc aacaatcatt ttgctcttgt tgactgtgct gttcatcttt ccattctact
    12481 ggattttgac aggggcattc aaatcacaac ctgatacaat tgttattcct cctcagtggt
    12541 tccctaaaat gccaaccatg gaaaacttcc aacaactcat ggtgcagaac cctgccttgc
    12601 aatggatgtg gaactcagta tttatctcat tggtaaccat gttcttagtc tgtgcaacct
    12661 catctctagc aggttatgta ttggctaaaa aacgtttcta tggtcaacgc attctatttg
    12721 ctatctttat cgctgctatg gcgcttccaa aacaagttgt ccttgtacca ttggtacgta
    12781 tcgtcaactt catgggaatc cacgatactc tctgggcagt tatcttgcct ttgattggat
    12841 ggccattcgg tgtcttcctc atgaaacagt tcagtgaaaa tatccctaca gagttgcttg
    12901 aatcagctaa aatcgacggt tgtggtgaga ttcgtacctt ctggagtgta gccttcccga
    12961 ttgtgaaacc agggtttgca gcccttgcaa tctttacctt catcaatact tggaatgact
    13021 acttcatgca attggtaatg ttgacttcac gtaacaattt gaccatctca cttggggttg
    13081 cgaccatgca ggctgaaatg gcaaccaact atggtttgat tatggcagga gctgcccttg
    13141 ctgctgttcc aatcgtcaca gtcttcctag tcttccaaaa atccttcaca cagggtatta
    13201 ctatgggagc ggtcaaagga taatactctg agaaaatctc ttcaaactac gtcagcgtca
    13261 ccttgccata cttaagtatt gcctgcggtt agcttcctag tttgttcttc aattttcatt
    13321 gagtatagga aaatcaatct atcaagatac agaagtatat tttatagatt tagagaatat
    13381 agaggttata agtgtctaca aaatggaggg tatgcagtta ctttatgaag ttttgtcaga
    13441 cacttataaa cttaagaatg gttttagtta actatcagaa acgaaggaaa gagtatgatt
    13501 tttgacgatt tgaaaaacat caccttttac aaagggattc atcccaattt agacaaggct
    13561 atcgactatc tctaccaaca tcgtaaagat tcattcgaat taggaaagta tgagattgat
    13621 ggagataaag tctttctagt tgttcaggaa aatgtcctca atcaagttga gaataatcga
    13681 tttgaacacc ataagaacta tgcagatttg catttgctga tagaagggca tgaatattcg
    13741 agctacggtt cacgtatcaa agacgaggca gtagcattcg acgaagcgag tgacattggc
    13801 tttgttcatt gtcatgaaca ctacccactc ttgttgggtt atcacaattt tgcgattttc
    13861 ttcccaggtg agccacatca gccaaatggt tatgcaggca tggaagaaaa ggttcgaaaa
    13921 tatctcttta aaattttgat tgattaaaaa taggatgaat tgtttttttg taaagctttg
    13981 ataatactct accatgaaat tgatctttgt gaggtagaga aatgagaata aaatatttaa
    14041 aaattggtat cttctaagta tgctgcaaga gctagtttct tagatggaca ggggattaca
    14101 gttgatgaga tggcttggat aattaggggc attgtgactg cattgattgg tagatacata
    14161 aaattaggta cttatgcagt taagtatggt attagtatgg cgcgctcgat cttaagtagg
    14221 gcagctgcaa ctgcggcagc aagagtagga ttattgacta agatttttgg atggatttta
    14281 cgaatagctg tgctcgcagc tgatgtatat ggtaattttg ccaacaatat tgctgtagct
    14341 tgggatgcac atgataaaat ccctaacaat ggtcgtataa acttttaaaa tgcgagaatg
    14401 aaagcacttt gtattttttt attgtatatg ttagcttgga cagtgcttgc aatgataatc
    14461 cgtggagggc tagatggatt tgatgaacac acttggcgta ctattttaat tgcttcgctg
    14521 tccgggatat atatagatta tatgtccata gataaaagta gaaaaaagtc caaaagaaaa
    14581 aagaaaaaat agatttgttc atggtaggga cttatgaaag ctttactgac aaaaaagaaa
    14641 acagtttaca aagaaaaatg atggaggagc aaacatggca caaaaaggag taagccttat
    14701 caaggcagca tttgatacag ataactttct catgcgtttt agtgagaaag tcttggacat
    14761 cgtgacagcc aatcttcttt ttgtcgtctc ttgtttgccc atcgtgacga ttggagtggc
    14821 taaaatcagc ctctacgaga ccatgttcga agttaagaag agcagacggg tgcctgtttt
    14881 taaaatctat ctaagatctt tcaagcaaaa tctgaaacta ggtcttcagc tgggtttaat
    14941 ggagttagga attgtgtttc ttaccctttc agatctctat cttttctggg gtcaaacagc
    15001 tctgcccttc caattgctga aagccatttg tctaggcatt ctgattttcc ttacgattgt
    15061 gatgttggct agctatccaa tcgcggcacg ttatgattta acttggaaag aagtgttaca
    15121 aaaagggctt atcttggcta gttttaactt tccttggttc ttcctcatgt tagccattct
    15181 tgtcctcatt gtgatggttc tttatctgtc cgccttcagt ctactcttag gtggctcagt
    15241 cttcctactt tttgggtttg gactattggt ctttatccag actggattga tggagaaaat
    15301 tttcgcaaaa taccaatagg agccttattt ctgaaactac tttcaaaggc tccaaacgct
    15361 attctataag cgagaaacta aaatctaagt gtctataaga tattgaaagc cttttcggta
    15421 aagtgtatac taaagaagta aagaaaaatc tgctttagca gaaaataaaa ataataggag
    15481 aaaatctatg tcagatttga aaaaatacga aggtgtcatt ccagccttct acgcatgtta
    15541 tgatgagcaa ggagaagtaa gtccagagcg tacgcgtgcc ttggttcaat acttcattga
    15601 taaaggtgtt caaggtcttt atgtcaatgg ttcttctggt gaatgtatct accaaagcgt
    15661 tgaagatcgc aagttgattt tggaagaagt catggcggta gccaaaggta aattgaccat
    15721 tattgcccat gttgcttgca ataatactaa agatagtatg gaacttgctc gccatgctga
    15781 aagcttggga gtagatgcta ttgcaacgat tccaccaatt tacttccgct tgccagaata
    15841 ctcagttgcc aaatactgga acgatattag ttctgcagct ccaaacacag actacgtgat
    15901 ttacaacatt cctcaattgg caggggttgc tttgacttca agcctttaca cagaaatgtt
    15961 gaaaaatcct cgtgttatcg gtgttaagaa ctcttcaatg ccagttcaag atatccaaac
    16021 ctttgtcagc cttggcgggg aagaccatat tgtctttaac ggtccagatg aacagttcct
    16081 aggtggccgc ctcatgggtg ctaaggctgg tatcggtggt acttatggtg ctatgccaga
    16141 actcttcttg aaactcaatc agttgattgc ggataaagat ttagaaacag cgcgtgaatt
    16201 gcagtatgct atcaacgcaa tcattggtaa actcactgct gcacatggaa atatgtacgg
    16261 tgtcatcaaa gaagttttga agatcaatga aggcttgaat attggttctg ttcgttcacc
    16321 attgacacca gtgactgaag acgatcgtcc agttgtagaa gcggctgcag ccttgattcg
    16381 tgaaaccaag gagcgcttcc tctaatctaa aaggaggtat ttatgacata ctacgttgca
    16441 attgatatcg gtggaactaa catcaagtat ggtttggttg atcaagaggg gcaacttctt
    16501 gaatcacatg aaatgccaac agaggcgcat aagggtggac ctcatatctt acaaaagacc
    16561 aaagatatcg tagctagtta tttagaaaaa ggcccagtag caggtgttgc catatcttct
    16621 gctgggatgg tggatccgga taagggtgag attttctatg ctgggccgca aatccctaac
    16681 tacgcaggca cccagttcaa aaaggaaatc gaagaaagct ttactattcc ttgtgagatt
    16741 gaaaatgatg tcaactgtgc aggtcttgct gaggcagtat ctggttcagg caagggagca
    16801 agtgtgacac tttgcttgac cattggaacc ggtatcggtg gttgcttgat tatggatagg
    16861 aaagtcttcc atggttttag taattcagcc tgtgaagtcg ggtatatgca tatgcaggat
    16921 ggagcttttc aggatctcgc ttctacaaca gctttagtga aatatgtagc tgaagcccat
    16981 ggagaagatg ttgatcagtg gaatggccga cgtatcttta aggaagctac tgaaggaaac
    17041 aaaatctgca tggaaggtat tgaccgtatg gttgactatc taggaaaagg tctggcaaat
    17101 atttgctacg ttgccaatcc agaagtggtc attcttggtg gtggtatcat ggggcaagag
    17161 gctatcctca aacctaagat ccgcacagcc ttgaaagagg ctttggtacc aagtttagca
    17221 gaaaaaacac gattagaatt tgcccatcac caaaatacag cagggatgtt gggtgcatat
    17281 tatcatttta agacaaaaca atcctagttt ggctcagcca aactaggatt ttcttacacg
    17341 tttttgtcta cgatagccgt tgagtttttt attttcccag tagctattaa agattttttc
    17401 cttgctttcg cgattgattt ccaaaaagta ggcataaatc aaatcgataa agaagagcat
    17461 aggaagttga gcggatattc gttggatata ggagggttgg ctgtgggtgg ctacaagaac
    17521 agtctctgta taggtctggc tatttttatt gggaacactt gtaaagagta cagtctttgc
    17581 ccccatctcc ttggcatcta atagactatc taaaatagaa ggagttgagc ctgaaagtga
    17641 gaagcccagt actagacaat tttcatccat gatgctggtt gtccaggcaa agccgtcttg
    17701 gtctgtcaaa gcttcgcaga ccacacctaa tcgcataaaa cgtaatttca tttcacgggc
    17761 gacgaggcca gaactccctg ttccaaagaa gtagatacgc tcagcatctt cgattagctg
    17821 ggcaattcgt tctagttgga tttcgtcaat caagtcttgt gtttgttccc tcatattgct
    17881 ataacttctg aggactcgtt tggtcagtgg actgtgcttg gaaacttggt tggcttgatt
    17941 ttctgcctga tgttggtatt ggaaaataaa ttctcggtag ccagtaaagc cacacttttt
    18001 agcaaagcgg gtcaaagcag cttgagaaat atgtaatttt tgggtgactt gttgagaaga
    18061 taaatcatct gtaatcgttt cagcttgcaa aaaatagcga gcgatttctt gctctaggtc
    18121 tgtcatttct tcaaaatgtg aatcaatgat agttgcgata tctggtttgt ccatagggaa
    18181 agctccttta catgagtcat actggaagac tagatcaggg aatagtcaca cttcattata
    18241 acacataata taaggataga taaataaaaa cgcatctctg ttttaaaaac gaaaaaatcg
    18301 aaaaagcttc tctcttttcc ataattttct actcaaattg tggtacaatt aagagtaaga
    18361 ttttaagtta gaaatgagac tgatttgtat gagaaaattt aacagccatt cgattccgat
    18421 tcggcttaat ttattgtttt caatcgtcat tttactcttt atgaccatta ttggtcgttt
    18481 gttgtatatg caggttttga acaaggattt ttacgaaaaa aagctagcct cagctagtca
    18541 gaccaagatt acaagcagtt cagcccgtgg ggaaatttat gatgctagtg gaaaaccttt
    18601 ggtagaaaat acgttaaagc aggttgtttc ctttacgcgt agcaataaaa tgacggctac
    18661 agacttaaaa gaaacagcta aaaagttact gacttatgtg agcatcagtt ctccaaattt
    18721 gacagaacgc cagctggcgg attactattt ggctgatcct gaaatctata aaaaaatagt
    18781 ggaagctctc ccaagcgaga aacgcttgga ttcagatggc aatcgtctat ccgaatcaga
    18841 actgtataac aatgcggtcg atagtgtaca aacgagtcaa ctaaactata cagaggatga
    18901 aaagaaagaa atctatcttt ttagtcagtt aaatgctgtt ggaaactttg cgacaggaac
    18961 cattgctaca gatcctctaa atgattctca ggtggctgtt attgcctcta tttcaaagga
    19021 gatgcctggc attagtattt ctacttcttg ggatagaaag gttttggaaa cttccctttc
    19081 ttctatagtt gggagtgtat ccagtgaaaa agctggtctc ccagcggaag aagcagaagc
    19141 ctatcttaaa aaaggctatt ctctaaatga tcgtgtagga acctcctatt tggaaaagca
    19201 atatgaagag accttacaag gaaaacgctc ggtaaaagaa atccatctgg ataaatatgg
    19261 caatatggaa agcgtggata caattgagga aggtagtaag ggaaacaata tcaaactgac
    19321 cattgatttg gctttccaag atagcgtgga tgctttactg aaaagttatt tcaattctga
    19381 gctagaaaat ggtggagcca agtattctga aggtgtctat gcagtcgccc ttaacccaaa
    19441 aacaggtgcg gttttgtcta tgtcagggat taaacatgac ttgaaaacgg gagagttgac
    19501 gcctgattcc ttgggaacgg taaccaatgt ctttgttcca ggttcggttg tcaaggcggc
    19561 gaccatcagc tcaggttggg aaaatggagt cttgtcagga aaccagacct tgacagacca
    19621 gtccattgtc ttccaaggtt cagctcccat caattcttgg tatactcagg cttacggttc
    19681 attccctatc acagcggtcc aagctctgga gtattcatca aatacctata tggtccaaac
    19741 agccttaggt cttatggggc aaacctatca acccaatatg tttgtcggca ccagcaatct
    19801 agagtctgct atggagaaac tgcgttcaac ctttggcgaa tatggcttgg gtactgcgac
    19861 aggaattgac ctaccagatg aatctactgg atttgttccc aaagagtata gctttgctaa
    19921 ttacattact aatgcctttg ggcagtttga taactatacg ccgatgcagt tggctcagta
    19981 tgtagcaact attgcaaata atggtgttcg tgtggctcct cgtattgttg aaggcattta
    20041 tggtaataat gataagggag gactgggtga cttgattcag caactgcaac cgacagagat
    20101 gaataaggtc aatatatccg actccgatat gagcatcttg caccaaggtt tttatcaggt
    20161 tgcccatggt actagtggat tgacaactgg acgtgccttt tcaaatggtg ccttggtatc
    20221 cattagcgga aaaacaggta cagccgaaag ctatgtggca gatggtcagc aagcaaccaa
    20281 taccaatgcg gtggcctatg ccccatctga taatccccaa atcgctgtcg cagtggtctt
    20341 tcctcataat accaatctaa caaatggtgt aggaccttcc attgcgcgtg acattatcaa
    20401 tctgtatcaa aaataccatc caatgaacta gaaaggaaat tatgctttat ccaacaccta
    20461 ttgccaagtt gattgacagt tattctaagt taccaggtat cgggattaag acggctacgc
    20521 gtctggcctt ttatacgatt gggatgtctg ctgatgatgt caatgaattt gcaaaaaatc
    20581 tcctttctgc taagagagaa ttgacatatt gttctatttg tggacgtttg acagacgacg
    20641 atccttgttc tatctgtact gattcgactc gtgaccagac aacaatttta gttcttgagg
    20701 atagtagaga tgtggcagcc atggaaaata tccaagaata ccatggactc tatcatgtcc
    20761 tgcatggcct catttctcct atgaatggta tcagtccgga cgatatcaat cttaagagcc
    20821 ttatgactcg tcttatggat agtgaggttt cagaagtgat tgtggcgact aatgctacag
    20881 cggatggtga agcgacctcc atgtatcttt cacgtttgct caagccggct ggtatcaagg
    20941 ttacgcgtct agcacgaggt ctcgctgtgg gagcggacat tgagtatgcg gacgaagtga
    21001 cactcttacg agcgattgaa aatcggacag agttgtaagt gtaggcaaat ttacgaactc
    21061 cattcattta taaaaaatca aagaggctga aaatcgttcc tatcggcctc tttttgtata
    21121 gtgtgatgag tagaatcagg ttcaagtttt aaaaaaccaa gcaaatatga tatactaaag
    21181 agcgagtatt ctagtagaat taggacaaat aatatgaaac aaacgattat tcttttatat
    21241 ggtggacgga gtgcggaacg cgaagtctct gtcctttcag ctgagagtgt catgcgtgcg
    21301 gtcaattacg accgtttcac agtcaagact ttctttatca gtcagtcagg tgactctatc
    21361 aaaacgcagg aatttagtca tgctccgggg caagaagacc gtctcatgac caatgaaacc
    21421 attgattggg ataagaaagt tgcaccaagt gctatctacg aagaaggtgc agtggtcttt
    21481 ccagtccttc acgggccaat gggagaagat ggctctgttc aaggattctt ggaagttttg
    21541 aaaatgcctt acgttggttg caacattttg tcatcaagtc ttgccatgga taaaatcacg
    21601 actaagcgtg ttctggaatc tgctggtatt gcccaagttc cttatgtggc tatcgttgaa
    21661 ggcgatgatg tgactgctaa aatagctgaa gtggaagaaa aattggctta tccagtcttc
    21721 actaagccgt caaacatggg gtctagtgtc ggtatttcta agtctgaaaa ccaagaagaa
    21781 ctccgtcaag ccttaaaact tgccttccga tatgacagcc gtgtcttggt tgagcaagga
    21841 gtgaatgccc gtgaaattga ggttggcctc ttgggtaact acgatgtcaa gagcacgcta
    21901 cctggagaag ttgtcaagga cgttgccttt tatgactacg atgccaagta tattgataac
    21961 aaggttacta tggatattcc tgccaaaatc agtgatgatg tggtggctgt catgcgtcaa
    22021 aatgcagaaa cagccttccg tgccattggt ggccttggtc tatctcgttg cgatttcttc
    22081 tatacagata agggagagat ttttctcaac gagctcaata ccatgccagg tttcacccag
    22141 tggtctatgt acccactact ttgggaaaat atggggatca gctacccaga actaatcgag
    22201 cgtttggttg accttgccaa ggaaagtttt gacaagcgcg aagcgcattt gatataaaat
    22261 gaaagagagg gtagaagcca gaaccatcac tgcaaggtga ctagagttct cggacttcag
    22321 ccctttttaa aggagtagaa atgaaattaa caatccatga aattgcccaa gttgttggag
    22381 ccaaaaatga tatcagtatc tttgaggaca ctcagttaga aaaagctgag tttgatagtc
    22441 gtttgattgg aactggagat ttatttgtgc cacttaaagg tgcgcgtgat ggccatgact
    22501 ttattgaaac agcctttgaa aatggtgcag cagtaacctt gtctgagaaa gaggtctcaa
    22561 atcatcctta cattttagta gatgatgttt tgacagcctt tcaatcccta gcatcctact
    22621 atcttgaaaa aacgactgtt gatgtctttg ctgttacagg ttcaaatggc aagacaacga
    22681 ctaaggatat gttggcgcat ttactgtcaa caagatacaa gacctacaaa acacaaggca
    22741 attacaataa tgagattggc cttccttaca cagttcttca tatgcctgaa ggaacagaaa
    22801 agttggtttt ggagatggga caggatcact tgggagatat tcatctcttg tctgaattgg
    22861 ctcgtccaaa aacagccatc gtgaccttgg ttggagaagc tcatttggcc tttttcaaag
    22921 atcgttctga gattgctaaa ggaaaaatgc aaattgcaga cggaatggct tcaggttcct
    22981 tgcttttagc gccggctgac cctatcgtag aggactattt gccaattgat aaaaaggtgg
    23041 ttcgttttgg tcaaggagca gagctggaaa ttactgactt ggttgagcgt aaggacagtc
    23101 tgacctttaa ggctaatttc ttggaacaag cccttgattt gccagtaact ggcaagtaca
    23161 atgcgacaaa tgctatgatt gcatcctatg ttgccttgca agaaggagtt tcagaggagc
    23221 aaattcgttt ggcattccaa catcttgaat tgacgcgtaa ccgtaccgag tggaagaaag
    23281 cagccaatgg agcagatatc ctgtcagatg tttacaatgc caatccaact gctatgaaac
    23341 tgattttaga gactttctct gccattccag ccaatgaagg tggcaagaaa attgcagtgt
    23401 tggcggatat gaaggagctt ggtgaccagt ctgttcaact tcataatcag atgattttga
    23461 gcctctcccc agatgtgctt gatatcgtta ttttctatgg agaagatatt gctcagttgg
    23521 ctcaattggc cagtcaaatg tttccaatcg gccacgttta ctacttcaag aaaacagaag
    23581 accaggatca atttgaagac ctagtcaagc aggttaagga aagccttgga gcctatgacc
    23641 aaatcttgct aaaaggttct aactctatga atctagccaa attggtagaa agtttagaaa
    23701 atgaatgcaa gtgattttgc taagtatctt caaagaatga ttgctcttac agatactgga
    23761 ctaaccttta caaaggatcc gtttgaccgt gagcgctacg aagacttgcg aagtctgtta
    23821 tctgaaatgt tgaatcaggt atcagacctt gatgcagacg aagtggcaga actcttgaaa
    23881 ccgacctccg cttatgcgac tcctttaatg gacgtccgtg cttggatagt tgaggatgaa
    23941 aaaatttgtc tggttagggg acaaggagag aatgattggg ctttgccagg tggctttggc
    24001 gaggtcggtt attcaccaac tgaaaatatt ctcaaggaaa ttgaagaaga aaccggtttt
    24061 aaagccaaag ttgaacgact actagctgtt tttgatacca atcgtttcca actacagagc
    24121 aaacaatatg caaagtttgt ttttgaatgt aagcttctcg atggacaatt tcaagaaaat
    24181 caagaaatcg ctgaccttca attttttgca attgaccaac tgccgaattt atctgaaaaa
    24241 cgcattacca aggagcaaat agagcttctt tggcaggttt atcaaggtca tagggggcaa
    24301 tatcttgact aagaagatga ttatcgtatt tctaaatcca tttttaacaa ctagcatggt
    24361 ataataatat gcaggaaaat tttgaattat gaggaagact agatgaattt atgggatatt
    24421 ttctttacga ctcaggcaac cgagccgccc aaatttgacc ttttttggta tgttagccta
    24481 tttacgctct tagccttaac cttttataca gcccatcgct atcgtgaaaa taaggtttac
    24541 caacgatttt tccaaatctt gcagactgtt cagttaatcc ttctttatgg ttggtactgg
    24601 gtcaatcata tgccactgtc agaaagccta cccttttacc attgccgtat ggctatgttt
    24661 gtggtactct tgcttcctgg tcaatccaaa tataaacaat actttgcatt attgggaaca
    24721 tttgggacat tagcagcctt tgtttatcca gtgccagatg cttacccttt tccacatatc
    24781 accattctat cctttatctt tggtcattta gcactcttgg ggaactctct agtttatcta
    24841 ttgagacagt ataatgcgcg attgctggat gtgaagggaa tttttctcat gacctttgcc
    24901 ctaaatgcct tgatttttgt ggtcaatttg gtgacaggtg gcgattacgg atttttgaca
    24961 aaaccgccat tggttgggga tcacggtcta gtagctaatt atttacttgt ttcaattgtg
    25021 ctggtagcta ctatcagttt gactaagaaa atcttagaat tctttttagc tcaagaagca
    25081 gaaaaaatga ttgcaaagga agcttaacac agagctttct tttttgctct tagagagttt
    25141 ttacaagcag cttataaaat aagaatttct gaatagacaa actcaaaaaa tggctgggaa
    25201 atttaggaaa aaagcaagca cgattaaatt ttttgtgtta taatattttg tgaatagcta
    25261 tgcctatgtt tagctatgga ataatacgaa gtgcgaaact tggaagatag agaggaagcg
    25321 atgtaatggc tagagaaggc ttttttacag gtctagatat tggaacaagc tctgtcaagg
    25381 tgcttgtggc cgagcagaga aatggtgaat taaatgtaat tggcgtgagt aatgccaaaa
    25441 gtaaaggtgt aaaggatgga attattgttg atattgatgc agcagcaact gctatcaagt
    25501 cagccatttc ccaagcggaa gaaaaggcag gcatttcgat taaatcagtg aatgtcggct
    25561 tgcctggtaa tcttttgcag gtagaaccaa ctcaggggat gattccagta acatctgata
    25621 ctaaggaaat tacggatcaa gatgttgaaa atgttgtcaa atcagctttg acaaagagta
    25681 tgacacctga ccgtgaagtc attaccttta ttcctgaaga atttattgtg gatggtttcc
    25741 aagggattcg tgacccacgt ggcatgatgg gggttcgcct tgaaatgcgt ggtttgcttt
    25801 atacaggacc tcgtactatc ttgcacaatt tgcgtaagac ggttgagcgt gcaggtgttc
    25861 aggttgaaaa tgttatcatt tcaccactag caatggttca gtctgttttg aacgaagggg
    25921 aacgtgaatt tggtgctaca gtgattgata tgggggcagg tcaaacgact gtcgctacaa
    25981 tccgtaatca agaactccag ttcacacata ttctccaaga aggtggagat tatgtaacta
    26041 aagatatctc caaggttttg aaaacctctc gcaaattagc ggaaggctta aaactgaatt
    26101 acggggaagc ctatccgcct cttgcaagca aagaaacctt ccaagtagag gttattggag
    26161 aagtagaagc agtcgaagtg acggaagcct acttgtcaga aattatttct gcacgaatca
    26221 agcacatcct tgaacaaatc aagcaagaat tagatagaag gcgtctattg gacctccctg
    26281 gtggtattgt cttaatcggt gggaatgcca ttttaccagg tatggttgag cttgctcagg
    26341 aagtctttgg cgtccgtgtc aagctttatg ttccaaatca agttggtatc cgtaatccag
    26401 cctttgcgca tgtgattagt ttatcagaat ttgcgggtca attaacagaa gttaatcttt
    26461 tggctcaggg agcgataaaa ggtgagaatg acttaagtca tcagccaatt agttttggtg
    26521 ggatgctgca aaaaacagct cagtttgtac aatcaacgcc tgttcaacca gctcctgctc
    26581 cagaagtaga gccggtggcg cctacagaac caatggcgga tttccaacaa gcttcacaaa
    26641 ataaaccgaa attagcagat cgtttccgtg gcttgatcgg aagcatgttt gacgaataaa
    26701 gaggaaaaat aaattatgac attttcattt gatacagctg ctgctcaagg ggcagtgatt
    26761 aaagtaattg gtgtcggtgg aggtggtggc aatgccatca accgtatggt cgacgaaggt
    26821 gttacaggcg tagaatttat cgcagcaaac acagatgtac aagcattgag tagtacaaaa
    26881 gctgagactg ttattcagtt gggacctaaa ttgactcgtg gtttgggtgc aggaggtcaa
    26941 cctgaggttg gtcgtaaagc cgctgaagaa agcgaagaaa cactgacgga agctattagt
    27001 ggtgccgata tggtcttcat cactgctggt atgggaggag gctctggaac tggagctgct
    27061 cctgttattg ctcgtatcgc caaagattta ggtgcgctta cagttggtgt tgtaacacgt
    27121 ccctttggtt ttgaaggaag taagcgtgga caatttgctg tagaaggaat caatcaactt
    27181 cgtgagcatg tagacactct attgattatc tcaaacaaca atttgcttga aattgttgat
    27241 aagaaaacac cgcttttgga ggctcttagc gaagcggata acgttcttcg tcaaggtgtt
    27301 caagggatta ccgatttgat taccaatcca ggattgatta accttgactt tgccgatgtg
    27361 aaaacggtaa tggcaaacaa agggaatgct cttatgggta ttggtatcgg tagtggagaa
    27421 gaacgtgtgg tagaagcggc acgtaaggca atctattcac cacttcttga aacaactatt
    27481 gacggtgctg aggatgttat cgtcaacgtt actggtggtc ttgacttaac cttgattgag
    27541 gcagaagagg cttcacaaat tgtgaaccag gcagcaggtc aaggagtgaa catctggctc
    27601 ggtacttcaa ttgatgaaag tatgcgtgat gaaattcgtg taacagttgt cgcaacgggt
    27661 gttcgtcaag accgcgtaga aaaggttgtg gctccacaag ctagatctgc tactaactac
    27721 cgtgagacag tgaaaccagc tcattcacat ggctttgatc gtcattttga tatggcagaa
    27781 acagttgaat tgccaaaaca aaatccacgt cgtttggaac caactcaggc atctgctttt
    27841 ggtgattggg atcttcgccg tgaatcgatt gttcgtacaa cagattcagt cgtttctcca
    27901 gtcgagcgct ttgaagcccc aatttcacaa gatgaagatg aattggatac acctccattt
    27961 ttcaaaaatc gttaagtaaa tgaatgtaaa agaaaataca gaacttgttt ttcgagaagt
    28021 tgcagaggct agtctgagtg ctcatcgaga gagtggttcg gtctctgtca ttgcagttac
    28081 caagtatgta gatgtaccga cagcggaagc cttgcttccg ctaggtgttc atcatatcgg
    28141 tgaaaatcgt gtagataagt ttctggaaaa atatgaagct ttaaaagatc gagatgtgac
    28201 ttggcatttg attggtacct tgcaaagacg taaggtgaaa gatgtcattc aatacgttga
    28261 ttatttccat gcattggact cagtaaagct agcaggggaa attcaaaaaa gaagtgaccg
    28321 agtcatcaag tgtttccttc aagtaaatat ttctaaagaa gaaagcaaac acggtttttc
    28381 gagagaggaa ctgctggaaa tcttgccaga gttagccaga ctagataaga ttgaatatgt
    28441 tggtttaatg acgatggcac cttttgaggc tagcagtgag cagttgaaag agattttcaa
    28501 ggcggcccaa gatttacaaa gagaaattca agagaaacaa attccaaata tgcctatgac
    28561 cgagttaagt atgggaatga gtcgtgatta taaagaagcg attcaattcg gttccacttt
    28621 tgttcgtata ggtacatcat tttttaagta ggagagaacc atgtctttaa aagatagatt
    28681 cgatagattt atagattatt ttacggagga tgaggattca agtctccctt atgaaaaaag
    28741 agatgagcct gtgtttactc cagtaaattc ttcacaggaa ccggctctcc caatgaatca
    28801 accttcacag tcggctggta caaaagagaa caatatcacc agacttcatg caagacaaca
    28861 ggaattggca aatcagagtc agcgtgcaac ggataaggtc attatagatg ttcgttatcc
    28921 tagaaaatat gaggatgcaa cagaaattgt tgatttattg gcaggaaacg aaagtatctt
    28981 gattgatttt cagtatatga cagaggtgca ggctcgtcgt tgtttggact atttggatgg
    29041 agcttgtcat gttttagctg gaaatttgaa aaaggtagct tctaccatgt atttgttgac
    29101 accagttaat gttattgtaa atgttgaaga tatccgttta ccagatgaag atcaacaggg
    29161 tgagttcggt tttgatatga agcgaaatag agtacgataa tgattttttt aattcgtatg
    29221 atttataatg cagtggatat ttactccctg attttggtag ccttcgctgt catgtcttgg
    29281 tttccaggtg cctacgaatc cagtttaggt cgttggattg tagcgttggt gaaaccagta
    29341 cttgctccct tgcaacgcct gcctttacag atagcgggtc ttgatttatc tgtttgggtc
    29401 gcgattgttt tggttcgatt tttaggagaa aacctagtgc gttttctggc gatgatagga
    29461 tgaataaagg gatttatcag catttctcca tagaagatcg tccatttctt gacaagggaa
    29521 tggaatggat aaagaaggta gaagatagct atgctccttt tttaactcct tttatcaatc
    29581 ctcatcagga gaagctatta aagattttgg ccaaaaccta tggtcttgct tgtagcagta
    29641 gtggggaatt cgtctcgagt gagtatgttc gagttttatt atacccagat tatttccaac
    29701 cagagttttc agattttgaa atatctctcc aagaaattgt gtattccaat aaatttgaac
    29761 atttaacgca tgctaagatt ttagggacag tcatcaatca attagggatt gaacggaaac
    29821 tttttggaga tatcctagta gatgaagaac gggcgcagat tatgattaat cagcagtttc
    29881 ttcttctctt tcaagatgga ctaaagaaaa ttggtcgtat acctgtttcg ctggaggaac
    29941 gtcctttcac cgagaaaata gataagctag aacagtatcg agaactggat ttatctgtgt
    30001 ctagttttcg attagatgtt cttttatcaa atgttttgaa actatctagg aatcaagcaa
    30061 accagttgat tgaaaagaaa cttgtccaag taaattatca tgtggtagac aaatcagatt
    30121 acactgttca agttggagac ttgattagtg tgagaaaatt tggtcgcttg agattacttc
    30181 aagataaggg acaaacgaaa aaagagaaga aaaaaataac cgtccagtta ttattaagta
    30241 agtgaggaat agaatgccaa ttacatcatt agaaataaag gacaagactt ttggaactcg
    30301 attcagaggt tttgatccag aagaagtcga tgaattttta gatattgtgg ttcgtgatta
    30361 cgaagatctt gtgcgtgcga atcatgataa aaatttgcgt attaagagtt tagaagagcg
    30421 tttgtcttac tttgatgaaa taaaagattc attgagccag tctgtattga ttgctcagga
    30481 tacagctgag agagtgaaac aggcggcgca tgaacgttca aacaatatca ttcatcaagc
    30541 agagcaagat gcgcaacgct tgttggaaga agctaaatat aaggcaaacg agattcttcg
    30601 tcaagcaact gataatgcta agaaagtcgc tgttgaaaca gaagaattga agaacaagag
    30661 ccgtgtcttc caccaacgtc tcaaatctac aattgagagt cagttggcta ttgttgaatc
    30721 ttcagattgg gaagatattc tccgtccaac agctacttat cttcaaacta gtgatgaagc
    30781 ctttaaagaa gtggttagcg aagtacttgg agaaccgatt ccagctccaa ttgaagaaga
    30841 accaattgat atgacacgtc agttctctca agcagaaatg gaagaattac aagctcgtat
    30901 tgaggtagcc gataaagaat tgtctgaatt tgaagctcag attaaacagg aagtggaaac
    30961 tccaactcct gtagtgagtc ctcaagttga agaagagcct ctgctcatcc agttggccca
    31021 atgtatgaag aaccagaagt agctccagtg catccgacag gtccaacacc agctacagaa
    31081 actgttgatt cagcaccagg atttgaagca ccgcaagaat ccgttacaat tttataagaa
    31141 acgatgtgag aacaatatct tatccttata tttccagcga gcaggagatg gtgtgagtcc
    31201 tgcattccct aacgataaga ttatcctctc aaaaactcaa gtctgaagct agtaagattt
    31261 gacgtttccc acgttacggg ataagaggga gaaagactaa atctttttcc gaataaaggt
    31321 ggtaccacga ttttcgtcct ttttggaagt cgtggttttt aatttgttat tatttataaa
    31381 ggagatacca tgaaactcaa agataccctt aatcttggga aaacagcttt cccaatgcgt
    31441 gcaggccttc cgaccaaaga gccagtttgg caaaaggaat gggaagatgc aaaactttac
    31501 caacgtcgtc aagaactaaa tgaagggaaa ccgcattttg tcttgcatga tggccctcca
    31561 tacgcaaacg gaaatatcca cgttggacat gccatgaatc atatctcaaa agatatcatc
    31621 attcgttcta agtctatgtc aggttttaac gcaccttata tcccaggttg ggatactcat
    31681 ggtctgccaa tcgagcaagt tttggcaaag caaggtgtga agcgcaaaga aatggacttg
    31741 gttgagtact tgaaactctg tcgtgagtac gctctttctc aagtttataa acaacgtgat
    31801 gatttcaaac gcttggggat gtcaggtgac tgggaaaatt tatatgtgac tttaactcca
    31861 gactatgaag cagcccaaat ccgtgtcttt ggtgagatgg ctaataaggg ttatatctac
    31921 cgtggtgcca agccagttta ctggtcatgg tcatctgagt cagcccttgc cgaagcggaa
    31981 attgaatacc atgatttggt ttcgacttca ctttattatg ccaataaggt aaaagatggc
    32041 aaaggtatcc tagatacaga tacttatatc gtagtctgga caacaactcc atttaccatc
    32101 acagcttctc gtggtttgac tgttggtgca gatattgatt acgttttggt tcaacctgct
    32161 agtgaaactc gtaagtttgt cgttgctgca gaattgttga ctagcttgtc tgagaaattt
    32221 ggctgggctg atgttcaagt gttggcaact taccgtggtc aagaactcaa ccacatcgta
    32281 acagaacacc catgggatac agctgtagat gagttggtaa tccttggtga tcacgttacc
    32341 actgactctg gtacaggtat cgtccataca gcccctggtt ttggtgagga cgactacaat
    32401 gttggtattg ctaatggtct tgaagtcgca gtgactgttg acgaacgcgg tatcatgatg
    32461 gcgaatgctg gtcctgagtt tgaaggtcag ttctacgaca aggttgtacc aactgttatt
    32521 gaaaaacttg gtaacctcct tctagcccaa gaagaaatct ctcactcata cccatttgac
    32581 tggcgtacta agaaaccaat catctggcgt gcagttccac aatggtttgc ctcagtttca
    32641 aaattccgcc aagaaatctt ggatgctatt gataaagtta aattccatac agaatggggt
    32701 aaagtacgtc tatataatat gattcgtgac cgtggtgact gggttatctc tcgtcaacgt
    32761 gcttggggtg ttccacttcc aatcttctat gcagaagacg gtacagctat catgacagct
    32821 gagactattg aacatgtggc gcaactcttt gaagtacatg gttcaagcat ttggtgggaa
    32881 cgtgatgcca aagacctctt accagaagga tttacacatc caggttctcc aaatggggag
    32941 ttcaagaaag aaacagatat tatggatgta tggtttgact caggttcatc atggaatggt
    33001 gttttggtaa atcgtccaaa tctgacttat ccagctgacc tttaccttga aggttctgac
    33061 caataccgtg gttggttcaa ctcatcactc attacatctg ttgccaacca tggcgtagca
    33121 ccttacaaac aaatcttgtc acaaggtttt acccttgatg gtaaaggtga gaagatgtct
    33181 aaatcacttg gaaataccat tgctccaagt gatgttgaaa aacaatttgg tgcagaaatc
    33241 ttgcgtctct gggtaacaag tgttgactca agcaatgacg tgcgtatctc tatggatatt
    33301 ttgagccaag tttctgaaac ttaccgtaag attcgtaaca ctcttcgttt cttgattgcc
    33361 aatacatctg actttaaccc agctcaagat gtagtcgctt acgatgaact tcgttcagtt
    33421 gataagtaca tgactattcg ctttaaccag cttgtcaaga ccattcgtga tgcctatgcg
    33481 gactttgaat tcttgacaat ctacaaggcc ttggtgaact ttatcaacgt tgacttgtca
    33541 gccttctacc ttgattttgc caaagatgtt gtttacattg aaggtgccaa atcattggaa
    33601 cgccgtcaaa tgcagactgt tttctatgac attcttgtca aaatcaccaa actcttgaca
    33661 ccaatccttc ctcacactgc ggaagaaatc tggtcatatc ttgagtttga agctgaagac
    33721 ttcgttcaat tgtcagaatt gccagaagct caaacttttg ctaatcaaga agaagtcttg
    33781 gatacatggg cagccttcat ggacttccgt ggacaagctc aaaaagcctt ggaagaagct
    33841 cgtaatgcaa aagttatcgg gaaatcgctt gaagcacact tgacagttta tccaaatgaa
    33901 gttgtgaaaa ctctactcga agcagtaaac agcaatgtgg ctcaactttt gatcgtgtca
    33961 gacttgacca tcgcagaagg gccagctcca gaagctgccc ttagcttcga agatgtagcc
    34021 ttcacagttg aacgcgctgc aggtcaagta tgtgaccgtt gccgtcgtat cgacccaaca
    34081 acagcagaac gcagctacca ggcagtcatc tgtgaccact gtgcaagcat cgtagaagaa
    34141 aactttgcgg aagcagtcgc agaaggattt gaagagaaat aaggttgaaa agtctaggca
    34201 aaattcaatt tgagaagaaa agacaactaa ttttatagtc tattaaacgc attgtatcac
    34261 gtttttgaat acctgatatg atgcgttttt tatttatttt aaaaatttgc gaggtatgac
    34321 tttttatact caacaagaat caaagagaaa cttagcaagc taacagtagt aagataaaat
    34381 aggaatttga tattagggat
//
LOCUS       DAVKFH010000020        33762 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKFH010000020 DAVKFH010000000
VERSION     DAVKFH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.020"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            82..270
                     /locus_tag="VW006_000995"
     CDS             82..270
                     /locus_tag="VW006_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379867.1"
                     /translation="MSFFAGFGAKLEFTSYNPFLSVKFGLVVALTVFVVQLLAILPAM
                     VKRGNKNENVESNNSCEE"
     gene            233..1624
                     /locus_tag="VW006_000996"
     CDS             233..1624
                     /locus_tag="VW006_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEU9379868.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            1880..2299
                     /locus_tag="VW006_000997"
     CDS             1880..2299
                     /locus_tag="VW006_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379869.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            2304..3323
                     /locus_tag="VW006_000998"
     CDS             2304..3323
                     /locus_tag="VW006_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU9379870.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            3327..3509
                     /locus_tag="VW006_000999"
     CDS             3327..3509
                     /locus_tag="VW006_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEU9379871.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            3511..4638
                     /locus_tag="VW006_001000"
     CDS             3511..4638
                     /locus_tag="VW006_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9379872.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLIFQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(4721..4793)
                     /locus_tag="VW006_001001"
     tRNA            complement(4721..4793)
                     /locus_tag="VW006_001001"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4758..4760),aa:Lys,seq:ctt)
     gene            complement(5058..6329)
                     /locus_tag="VW006_001002"
     CDS             complement(5058..6329)
                     /locus_tag="VW006_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9379873.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            6599..6799
                     /locus_tag="VW006_001003"
     CDS             6599..6799
                     /locus_tag="VW006_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379874.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            7003..7182
                     /locus_tag="VW006_001004"
     CDS             7003..7182
                     /locus_tag="VW006_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379875.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            7319..7498
                     /locus_tag="VW006_001005"
     CDS             7319..7498
                     /locus_tag="VW006_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9379876.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            7535..7987
                     /locus_tag="VW006_001006"
     CDS             7535..7987
                     /locus_tag="VW006_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9379877.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            8278..8748
                     /locus_tag="VW006_001007"
     CDS             8278..8748
                     /locus_tag="VW006_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9379878.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            8767..9855
                     /locus_tag="VW006_001008"
     CDS             8767..9855
                     /locus_tag="VW006_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9379879.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            9836..10264
                     /locus_tag="VW006_001009"
     CDS             9836..10264
                     /locus_tag="VW006_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9379880.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            10402..11352
                     /locus_tag="VW006_001010"
     CDS             10402..11352
                     /locus_tag="VW006_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9379881.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            11354..12097
                     /locus_tag="VW006_001011"
     CDS             11354..12097
                     /locus_tag="VW006_001011"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9379882.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            12108..13904
                     /gene="pepF"
                     /locus_tag="VW006_001012"
     CDS             12108..13904
                     /gene="pepF"
                     /locus_tag="VW006_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9379883.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(13964..14641)
                     /locus_tag="VW006_001013"
     CDS             complement(13964..14641)
                     /locus_tag="VW006_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379884.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQKKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEQGKEF
                     LEKEWGI"
     gene            14888..15658
                     /locus_tag="VW006_001014"
     CDS             14888..15658
                     /locus_tag="VW006_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379885.1"
                     /translation="MLYLLGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDFLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFWGLTGTEKEDLHV"
     gene            15651..16415
                     /locus_tag="VW006_001015"
     CDS             15651..16415
                     /locus_tag="VW006_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379886.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            16840..17631
                     /locus_tag="VW006_001016"
     CDS             16840..17631
                     /locus_tag="VW006_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9379887.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLVFTSF
                     AKEIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            17742..18410
                     /locus_tag="VW006_001017"
     CDS             17742..18410
                     /locus_tag="VW006_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9379888.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFANKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLIIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(18435..18794)
                     /locus_tag="VW006_001018"
     CDS             complement(18435..18794)
                     /locus_tag="VW006_001018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379889.1"
                     /translation="MLKAVFEVVDKTDEVSLKHCFEVADRTDEVSSKHCFEVADRTDE
                     VSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVVDETDEVSNHTYGKVKLTW
                     FEEIFEEYHTKKPCSSR"
     gene            complement(18844..19377)
                     /gene="queF"
                     /locus_tag="VW006_001019"
     CDS             complement(18844..19377)
                     /gene="queF"
                     /locus_tag="VW006_001019"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9379890.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(19682..19996)
                     /gene="trxA"
                     /locus_tag="VW006_001020"
     CDS             complement(19682..19996)
                     /gene="trxA"
                     /locus_tag="VW006_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9379891.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(20013..20234)
                     /locus_tag="VW006_001021"
     CDS             complement(20013..20234)
                     /locus_tag="VW006_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9379892.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(20333..20713)
                     /locus_tag="VW006_001022"
     CDS             complement(20333..20713)
                     /locus_tag="VW006_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9379893.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETVAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKILASLQKTVEESLEAGLDFLI"
     gene            complement(20856..21809)
                     /locus_tag="VW006_001023"
     CDS             complement(20856..21809)
                     /locus_tag="VW006_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9379894.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(21877..23086)
                     /gene="sstT"
                     /locus_tag="VW006_001024"
                     /pseudo
     CDS             complement(21877..23086)
                     /gene="sstT"
                     /locus_tag="VW006_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            23225..23785
                     /locus_tag="VW006_001025"
     CDS             23225..23785
                     /locus_tag="VW006_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9379895.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            23842..24750
                     /locus_tag="VW006_001026"
     CDS             23842..24750
                     /locus_tag="VW006_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9379896.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDKPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            24702..25292
                     /locus_tag="VW006_001027"
     CDS             24702..25292
                     /locus_tag="VW006_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9379897.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            25295..26401
                     /gene="yqeH"
                     /locus_tag="VW006_001028"
     CDS             25295..26401
                     /gene="yqeH"
                     /locus_tag="VW006_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9379898.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFITGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            26615..26926
                     /gene="yhbY"
                     /locus_tag="VW006_001029"
     CDS             26615..26926
                     /gene="yhbY"
                     /locus_tag="VW006_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9379899.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            26978..27607
                     /locus_tag="VW006_001030"
     CDS             26978..27607
                     /locus_tag="VW006_001030"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9379900.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            27607..28200
                     /gene="yqeK"
                     /locus_tag="VW006_001031"
     CDS             27607..28200
                     /gene="yqeK"
                     /locus_tag="VW006_001031"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9379901.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            28201..28701
                     /locus_tag="VW006_001032"
     CDS             28201..28701
                     /locus_tag="VW006_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9379902.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            28714..29067
                     /gene="rsfS"
                     /locus_tag="VW006_001033"
     CDS             28714..29067
                     /gene="rsfS"
                     /locus_tag="VW006_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9379903.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            29387..30127
                     /locus_tag="VW006_001034"
     CDS             29387..30127
                     /locus_tag="VW006_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9379904.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            30137..31234
                     /locus_tag="VW006_001035"
     CDS             30137..31234
                     /locus_tag="VW006_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9379905.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILAQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNLSIAQQNFGRVPIRIETN"
     gene            31364..32253
                     /locus_tag="VW006_001036"
                     /pseudo
     CDS             31364..32253
                     /locus_tag="VW006_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32470..32973
                     /locus_tag="VW006_001037"
                     /pseudo
     CDS             32470..32973
                     /locus_tag="VW006_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370715.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            32927..33067
                     /locus_tag="VW006_001038"
     CDS             32927..33067
                     /locus_tag="VW006_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379906.1"
                     /translation="MLFYLTKELNSGVKKIGTISTTLVVVAGVSAGVLGGKFDFKFPK
                     KI"
     gene            33083..>33762
                     /locus_tag="VW006_001039"
     CDS             33083..>33762
                     /locus_tag="VW006_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379907.1"
                     /translation="MKFKNYLFDLYPYFPKGFSLDNREDPDVCSKALYDDLCKMFFDD
                     DSKEKLKITSVCNKCQNYGNRDYYTLFIDKDKYLLSSDYIGASIYWAQEAGLNDRIIL
                     DHLSISRTIGGHILFPRGGKLETVNQARGGEKGYYDRFDLTLYAIKEWFVENKNTKIG
                     YAIENYHEWFELFSGDDNCKNGFENFVEFFKLEGFIYEQNKIIDLIKSDLENNQVVFL
                     DKEDILIA"
BASE COUNT        10166 a         6053 c         7543 g        10000 t
ORIGIN      
        1 ttaaaatttg aaaggaaaaa tacaaaaatg aaaattaatg aatatttatg gagaacgagc
       61 tgtcttatac tgctaattat catgagcttc tttgcaggct tcggagctaa attagagttt
      121 acatcttata acccgttttt atctgttaaa tttggtttgg tcgtagctct gacagtcttt
      181 gtagttcaat tgttagccat actgccagcc atggttaaaa gaggaaataa aaatgagaat
      241 gttgaatcaa acaattcgtg tgaagagtaa atacccttac ttgattcgtc cccgaaatat
      301 taaaattgta agattgttta tccaaatttt tgtcttttta ctcttaatat cttcgtggat
      361 tgctataatc ccatttattg ctcgtctagt cttttccatg ttttttataa gtcttctttt
      421 tttcgtagag aagttctttc gttctaattt tatagcaaaa ttgagaaaca agctcaatat
      481 cagagaagcc ttgttttaca tgttaattag tttaaattta tatgatgagc tcgataatga
      541 agtagtagat acagcagttt tatattttga cgttgagaat aataaagttg ttgtctgtgc
      601 cccactattt gggaaccgtt atctgaaaac cttgaaaaat ttagaggaat atctctgtcc
      661 cactttgggc ttgtccttgc tgtctaaaaa agaagaaatc gataaaatag tatacgtgtt
      721 agggcagaaa gaagaaatag aacaatatgt ttttaatagt aacacattaa caagagaatt
      781 ttttaaagat attcctagtt caataatcaa gctgtcaaat acacagaaat ttagtctaaa
      841 gtccaacact aacctaggta tatatggaag aacggggaca ggcaaaacaa tagctctgca
      901 atggtactta tttaacgcac tggctaaagg ttgtggtatt gctgataata cttatttagg
      961 catagtagat ggtaaggcag ctgacttgta tcgtattggt gaacttcttc acgaagaact
     1021 gggagaacaa gtggcagtag gctcttcacc acaaatgctt gcccaattat cgagaaaatt
     1081 cattgagaat atggatgcaa gatttaaaat tatcaaacag aatagttcat tgaatgctga
     1141 catttatgag ctagatgagc tgggactggt acctaatttt ttgttcgttg atgaactcgc
     1201 ttctattcgt gatagttgtg gctctacgaa acaaggtaga gaaacatgga atgaaattct
     1261 acaaaattta ggcttgattg cacgcaaagg acggcaggca ggttgccacc tagtcctatc
     1321 tactcaagac cctaatgccg aaaacatccc cgttgaattg aggaatcaaa tttcagcagt
     1381 tttatacctt ggtgccccag gggctgatag attgaagatg gcttttagca tgtgcgaatt
     1441 agaaaacgtt cctacagttt cagatagaaa aggggaggct ctcttttatg ctgatgggct
     1501 taatacagta gagccagtgc taacaattgt gccatttgtt gatttaaaaa ctaagaaaga
     1561 gtttttaaat gtagttagaa acatactgcc aactaatgac ggtcttcctc ccctagcgaa
     1621 atagggcgat tgccggaggc agaatcgagc ggagcgctct cttttcgcta ggggagggag
     1681 agccaaccga ctgctttgca ggaggtcaga aaaaacatcc ccagtactaa tagggggtgc
     1741 acacatacat gaaatgtact ctaaaccctt gatacgtgag gggttacggc ttactagttg
     1801 caccaagggg tcgcaaactc ttgggagagt agggatgaga ggtgtttcca tgtatgatac
     1861 ggaattaaag gagaaaaata tggtttacaa tatgacagat acaaacatta aaattggatt
     1921 gcttgacctt gagatgtcac agtttgatat tcctaagaaa gtagtgattt tatcagttag
     1981 ggtgaacaat aaatacaaag aagtcaacgg cgaaaacgtg aagacagatg aagtaagtaa
     2041 aattacttct gctgttcttg acgctgacaa agtgaaagtt ctaactgaaa tgggaatttc
     2101 tactgatgac ttaaaagcta ttaacctaga aattatcgga aatctagata agattgcaaa
     2161 acttgctcaa aatgatgctt tgttgaatgt tcctgttgaa ttgattcggc ctaaagttcg
     2221 actagcttgg aacatggcta ggaataattg gtctggtgta aaaattgtgt gtgaggatat
     2281 taaagttcta ggagaataat cctatgaata cagttagaat agattatttt gctgtgacag
     2341 ttaagaatat ttcgccagaa aaagttctga cagacatcct tttgattcct cttgaaaact
     2401 ttaccttgaa tgattggggc ataaataagt ataagcaaca ttattcttgt tcagaaatta
     2461 aggtgtattt taatgctgat aggctttcta tgggtgtatt tattgaatta aaagggcaag
     2521 gttgtcgtca atatgaagaa tttttaaaca gtaacgaaaa taattggatt gctttgataa
     2581 gtcgtttata tcatcataat gccaacttta ctagactaga tattgcccac gacatatttg
     2641 acggtagctt agatgttcaa cgaatatatg actattgcaa gaaaggatta tgtatctcga
     2701 aagcaaaaca ctttgaatat catgagaaat cagttctaga ttctggtgag cgtgtaggag
     2761 aaacagttgc aataggttca cgaggtaatc agcagtggtg catatataat aaacgcatgg
     2821 agcaacttgg taagcaagag cttgtagaag tttcatcttg gactcgcgca gagcttagat
     2881 gttggcagga gaaggctaat ataattgcag agcagttata tttaaaacgt cccttatcgt
     2941 caatatattt tgaggctatc aacggtcatt atcgctttat tcgtccaaat gctatagata
     3001 gtaatcgttg gagaagaaaa aaggttgagt ggtgggtaaa ctatctaggg acagaaaatc
     3061 agacagttct gagtattaca agggctaaga cgacgttacg ggagtctgaa gtttggactg
     3121 agaaacaggt tgcaaaaaca ttagcaaaag tctatattgc aaagtatcag gcctatgatg
     3181 ttcaaaaggc ggaagattat attcaaaact tgctcaaaga gggactttca aagctaactg
     3241 ataatgatga aaaggatata gaacaatata taagagaaca gaatagttcc caactttggg
     3301 gaaccgatgg aaggagtgac tgacatatgg cagatactat atcaaataag gatttgattt
     3361 ccatgggcta taggccatca acagctaatg ctattatcca tcaggcgaga gaattacttg
     3421 tatcacgagg ctatacattt tataatcgca aacgtttgat ggttgttcca aaaagtgttg
     3481 tgaaagagtt gttgggaatg gaattgtgaa atggcttata tcgagtataa acagcgtggt
     3541 aagaaaagac tttggtcctt ctctatacga gaaaggagca agagcctact ccataaaagc
     3601 ggatttaaaa caaaacgaga agctaaaata gaagcggaga aagttcttca taagttaaat
     3661 actgggagtg tcttaagctc tagtatgact ttatcggagc tttataatga atggcttgat
     3721 ttaaaaattt tacctagcaa tagaagtgta gttactaaaa aaaaatatct catgagaaag
     3781 aaggtcatcg aaaggttatt tggaaataag cctgtatcac aaattaagcc tagcgagtat
     3841 caaaaaatta tgaatgagta tggagagact gtatcgagaa atttcttagg aagattgaat
     3901 tcttgtattc gggcaagtat acagatggct attgctgata aggtgataat agaggatttt
     3961 actgcttatg ttgagttgtt ctcttctaag agtggacaaa aggttgagga aaagtatttg
     4021 catgctgagt cagattatca aaaagtttta gtatatttga aaaataagtt tgattatcaa
     4081 aaatctattg taccgtatgt aatttatttc ctttttaaaa ctggcatgcg tttctccgaa
     4141 ttgattgctc taacttggga tgaagttgac gagcttaatg agcaacttaa aacttatcgt
     4201 cgttacaaca ccgctattca taagtttact ccacctaaaa acaatacttc aataagatta
     4261 gttcctatta cttcagatat gctttccttg ttaaaaacat tgaaaatatt gcaattaaaa
     4321 acaaataaag aattaaatat tgataatgaa aataatttga tttttcagca ttttggctat
     4381 gtctacgatg ttccagatat tgcaacagtc aataaagcta taaaagtaat gttgaaggaa
     4441 ttgcaaattt ttccattgat tactaccaaa ggagctagac acacttatgg tagttattta
     4501 tggcataata atattgattt gggagttata gcgaaaattt taggacataa agatatctcc
     4561 atgttgattg acgtttatgg gcataccttg gaggaaaaaa ttagtgaaga gtttatggct
     4621 gtgaaaagtt tgttgtaaaa acacggcaaa ttgcacgaca atttttaaaa ggaaggcaaa
     4681 aaaagcccta tattataagg cttttcctgt tgtatttata tgccccctac agggatcgaa
     4741 cctgtgaccc acggattaag agtccgctgc tctgccagct gagctaagga ggcaaagaaa
     4801 aaagctgtat tggtgccgaa acttcacggt ttgtattgaa cccgcgcaat taagcaggtg
     4861 ggcaactcgc tctaactgaa gctgtttccg tgtgagacgg cctacatgct gttagaagac
     4921 ttttgtttcc ctaataatac aaaaaatagt cggtcaacac ttaagtgtga agtcgtacac
     4981 cacagcgttt ctatacttat atgataccac tttttcaaaa aaaatcaaga ggaaagtgca
     5041 attttttgaa aaggatttca gatttttcgc aaacggtcga tagcttcctt tctttgagcc
     5101 aaagcccgtt catatttacc agtatcttct gcttggaaat agtgatgatt acgaattttt
     5161 tctggcagat agtcttgctt gacccaattt ccaggatagt tgtgtggata gagatagtct
     5221 tgggcattcc ccagttcctt gcttccactg tagtgcccat cacgtaggtg tcgcggaata
     5281 ggcaagtgcc ctgatgtttt gaggtcagca agtgccttat ccatagctac ataggctgag
     5341 ttggattttg gagaaagggc caaatcaatc acgacattgg caatgagaat gcgggcttct
     5401 gggaacccaa tcttctgggc agcatccaga gcagtcacgg tatgaatctg ggcctcagga
     5461 ttggctaagc cgatatcttc ataggcgata acagtcaagc gacgagcgag actaggcaga
     5521 tccccagcct caatcaagcg ggcagtatag tggagactgg catccacatc tgagccacga
     5581 atagactttt gcagggcaga tagaacatca tagtgaccgt ctccatcctt gtccatagtg
     5641 atgtagcttc tctgaagact attttccatg atgtctaagg tgatgtggcg aatgccctcg
     5701 tcattctcag gggtagagag aacagctaag tccagtgagt taaaggcaga gcgaaggtct
     5761 ccgtttgtag aggttgcgat gaaatccagc gcatcctcat ctagttctat tggaaaatca
     5821 aaaccacgtt cagggttact tagagctatc tgcagggcct ctttgacgtc ttggttagac
     5881 agaggttcca actcgaaaat ttgaactcgg ctacgaatgg caggagtgac agagaagaaa
     5941 ggattttcag tcgttgctcc aatcatgatg accagtccac tttccaagag aggcaagagg
     6001 aagtcttgct tggtcttatc tagtcgatga atttcgtcta gcaataggac gagaccacca
     6061 gaaaatttag cttcttccga gatttcttgc agtcgctttt tactatcaac tgtcgcattg
     6121 aaagttcgaa aggcatactt ggtcgttcca gcgatggcag aggcaatact ggttttgccg
     6181 attccaggag ggccatatag aatcatggag gacaggcggt tggcttccac catgcggcgg
     6241 atgatttttc caggtccgac cagatgctcc tgaccgatga cctggtcgat ggttttaggg
     6301 cgcatgcgaa gcgcgagatt gtctggcata gcagtccttt ctagcgtgga tttgagtaga
     6361 atgactcatc tactatatct tactatgtcc attctagcct attagttgtg atatttcaat
     6421 ggttttgtaa taatcaggga gtgagataga atctttttta ctatttaatt taatgaggag
     6481 aggtatcagc atgagagatt tgattttcct taaagctcaa aataggatag ttgagcctaa
     6541 agaagtggaa gtgctgactt gagaaataat attgctgaat gtcattgtat gaactataat
     6601 gaaaagagag gtgataagca atggaaatga tggaccaagt caggaaattt tgatttttac
     6661 aaaacaaatt cgtcattgga ttcttagtga tcaagttatt tcagggaaaa gaaaactttt
     6721 cttccgggaa gatactccaa aagagatttt agatttgtac gaaaacatta aatctaaact
     6781 tgattttgct taccaataag tttattctaa taatgggttg aaaaaatatg aaaaatagaa
     6841 taattgatgt ttttgaagta gtaaatcgca tccttgtaat aactgttgaa aatcctgatt
     6901 tcgaagactt gagagttaat catactcaat gaaaatcaaa gagcaaacta ggaaactagc
     6961 cgcaggctgt acttgagtac ggtaaggcga cgttgacgtg gtttgaattt gattttcgaa
     7021 gagtatcagt ttgtaaaaat tggagataaa aaatatagag tacgcagtgt tcctatgatt
     7081 cattcaactc cgcctcaatc tgtcttagat cgagatactt ttacaattga ctatacagat
     7141 gatgaattgc tcgataaaga agcagttttt actactcatt agaaaaatag gtctgaataa
     7201 attggcttcc cttagaacca catctagggg cttttatgta taatagaata ataagtatca
     7261 ctcctttaca atacgtgtaa cacgtgttat aatggtgttg tcaggaggta aaagcgctat
     7321 gcctatgaca caaaaagaga tggttaaact ccttacggcc catggttgga taaagactag
     7381 aggcggtaaa ggctcccata ttaaaatgga gaagcaaggg gaaagactta tcacaatccc
     7441 tcatggtgag ctgaataagt acactgaaag agggataaga aagcaagctg ggttgtaaaa
     7501 ttcagcccct gttttttgat atggaggtag tgaaatgtta gttacgtatc cagctttatt
     7561 ttattatgac gacacagacg gaacagaagc gacttatttt gtccatttcc cagattttga
     7621 atactcagct acacaaggag aggggatttc tgaggctttg gctatggggt cggagtggct
     7681 agggataact gttgcagatt tgatagagag tgatggagag ttacctcagc catcagatat
     7741 caatagcttg tctttaattg ataatgatcc ttttaaagat gatgaagatt tcgtgtcaac
     7801 ctatgacctt gataaatctt ttatttctat ggtatctgtt gatgtatcag aatacctagg
     7861 aagtcaggaa cccataaaaa agactttgac cataccaaaa tgggcagata agttgggacg
     7921 agaaatggga cttaactttt ctcagacttt gacagacgct attgcagata agaaagttca
     7981 agcctaagat taagcgtggt atcgtggtaa tagtcaataa aaaagcacgt ccacttgtgc
     8041 tagttactcg cctattgatt ttatagattt attagccctt tcgcgggctt tttatattga
     8101 tttttatgag aaatgaagaa atcagacttt taagaaaatc actgatacca agggtttaaa
     8161 tgaggtaata tggtataatt aggacataaa ataattttgt ggtaagatgg tagtatctat
     8221 tttagcatat ttccgagcaa tcggggcgat taaagagtcg catagaaaga ggacaaaatg
     8281 gcaacatacg gatttttaga tgttttagag gaagagttgg acaagaactt tccctttgac
     8341 tttgagatta gctgggacaa acgcaaccat gcggtagaag tgagttttct attggaagcg
     8401 caaaatgctg caggtgtgga gatggtggat gaagacggag aggttttatc agatgacatt
     8461 ctttttgagg aagcggtcct tttctacaat cctgccaagt caacagtcaa tgaggaagac
     8521 tatttgactg ttattcctta cctgcctaaa aaaggatttt cccgtgaatt tttagcttat
     8581 tttgcgcttt tccttaaaga tactgctgag gttgggctag atgtcctcat ggactttttg
     8641 gaagacccag aagcagagga atttgtcatg gaatggaacc aagaagtctt tgaagaagga
     8701 aaagttggct tggaagaggg agaattttat ccttatccaa gatactagga gttggttgga
     8761 ggtgttatga agaaaattgg gacttatttg gtttatgtgc tagcttttgt ctttattatg
     8821 ctagcttttg cttgtggaac aatcgcattt gcagagttgg ggtattccgc agttttagcc
     8881 tttacttttg gttatgcctt cgctcttcta agcatgtatt taatctttat tcttcatgag
     8941 ctgggtcatg ctttttgtgg ctacttgaca ggctatcggc tggtggcttt tggattagga
     9001 cattttattt tgaccaaaaa gtcaggcagg tttcatctta gtagaacagc cattctgaaa
     9061 aatgttggtg ctcaatacat tggtttaaaa gaggatgaaa gcgatcaaag aatcatcctg
     9121 atgctttcag gaggcttgat ggttcatctc ggcttgatat tattggcgat agtgtttgga
     9181 tttttgacaa gaagctggta ttttgcaggg acttggattt ttcttaattt atctttcttc
     9241 ctaaataaca ttttgccagt cgacatcacc gatggagcaa aaatttggga attactacaa
     9301 caccctgaaa ataccaaata cgcctacctg gtattgagac attctgctca aactttgctg
     9361 gctcctcaag aatatgattt gaaagacttt gttcaagcag ttcctgagga tgcacgaggg
     9421 agttttgcgg atggtgtatt aggtatgcaa ggtgaagttt ccattttgga agggaaggaa
     9481 gaagtagcta aacagcagtt tcaagcatta cttgagagga caactactcc gatgatgcag
     9541 acagttgccc aactatccct gcttcatatc gccttgttgg aaggagattt tgagcaagcg
     9601 gagcaatatg ctagtattcg acaggtcaaa tcgtttctgt ctctcaaact gtccaatctt
     9661 caagtgatac aagcttggta tcaatttaag gtaaagaaag atgtagatcg aactcgtaag
     9721 gctctcaaga ttgctagaca gaaaatggat agtagtcgaa tgttgaggga cgagaaacgc
     9781 tattatgaaa acttgttagc agagctggaa aaggaaatgt tagaaggaac tgaagatgga
     9841 actagaaatt tctgatttcc caggctgtaa gattgctttg ttttgtgggg ataagctctt
     9901 gactatctta cgtgatgata aggcaagtat tccctgggcc aatatgtggg aactgccagg
     9961 tggtggacgt gaaggtgatg aaagtccttt tgagtgtgta gcgcgtgaag tttatgaaga
    10021 actgggcatt catctgactg aagattgtct gctttggagt aaggtttacc caagtatgct
    10081 ttttgcagac aaacagtccg tttttctagt tggtcagttg acacagaacc agtttgacag
    10141 tatcgtattt ggagatgaag gacagggcta tcagctgatg aatgttgagg aatttcttag
    10201 ttccagtcaa gttgtccctc agttgcaaga gagattaaaa gattatttaa aagtaagtga
    10261 ttagaaagga tggttcagtt aaatttctaa actgaacccg ccctaaacac tgtgccaaaa
    10321 agataaactt ctcttagaca caagcgtctt cagagaattt cctattttgg ttttgtgttt
    10381 tacgggcttg gtatcttaat aatggaaaca tggcaagagt taaaagttac agtgaagcgt
    10441 gagggagagg agttggtttc caatctcttg attgagctgg gagcgcaagg agtcgcaatc
    10501 gaagacagta tggactatgt gggaaatgtt gaccgctttg gcgagatttt cccagaggtg
    10561 gagcagcaag aagaaatcgt tgtgacagcc tactaccctg acacggtgga tgtgacagtg
    10621 gttgaggcgg acttgcaggc tcgcatatca gaattgacgg attttatgga tttgggagag
    10681 ttaaaaatag gtacgactgc cttggctgag gaagactggg cagacaactg gaagaaatac
    10741 tatgagccag ctcgtatcac tcatgatttg accatcgttc cctcttggac agactatgag
    10801 gcgactgcgg gagaaaaaat tatcaagctg gatcctggca tggcttttgg tactggaacc
    10861 cacccaacta ccaagatgag cctttttgcc ttggaacagg ttcttcgtgg tggcgaaacg
    10921 gtgctagatg tggggactgg ttcaggggtt ctctctattg ccagctcgct tctgggggct
    10981 aaagaaattt tcgcctatga cctggatgat gtggcggttc gtgtggctca ggaaaatatt
    11041 gagctcaacc ctggcatgga aaatatccat gtagcggctg gagatttgct taagggtgtg
    11101 gaaattgagg cagatgtgat tgtggctaat atcttggcgg atatcctcat tcatctaata
    11161 gacgatgcct atcgtttggt taaggacgaa ggctacctga tcatgagtgg cattatcaag
    11221 gacaagtggg acatggtgcg ccagtcggct gagtcagctg gatttttcct cgaaactcac
    11281 atggttcaag gggaatggaa tacctgtgtc tttaagaaaa ccaaggatat ctctggtgtg
    11341 attggaggct agcatgcagc agtattttgt aaaaggcagt gctatctctc ctgtcactat
    11401 cgaggacaag gaaaccagta agcatatgtt tcaggttatg cgcttgaaag aagaggatga
    11461 ggttacctta gtctttgatg atggcatcaa gcgcttggcg cgcgtgctgg atatggaaaa
    11521 tcgtcagttt gagttggtcc aagaattaaa tgacaatgtg gaactaccag tccaagtgac
    11581 catcgcatct ggatttccca agggagacaa gctggagttc atcgctcaaa aagtaaccga
    11641 actgggtgct agccaaatct gggcctttcc tgcagactgg tcagttgcca agtgggatgg
    11701 caagaaattg ggtaaaaaag ttgaaaaact agaaaaaatt gcccttggag cagccgagca
    11761 aagcaagcgt aatattgtac caagtattaa acttttcgag aaaaaagcag attttctagc
    11821 tcagctggac cagtttgact ttatcatagt agcctatgaa gaatcagcta aagaaggaga
    11881 agccgctgcg cttctgcaag cagtcactgg tcttgaaaca ggagccaaac tgctctttat
    11941 ctttggtcca gaaggcggtc tctcacctgc agaaatcgaa agttttgaag ctaagggagc
    12001 cgttttggta ggcctaggtc ctcgtatttt gcgagcagaa acagcaccgc tttacgcctt
    12061 atcagccctt agtgttttag tagaattaga gaaataagag gaagaaaatg gaacaaaaac
    12121 accgttcaga atttccagag aaggaactct gggacttaac agccctatac caagaccgtg
    12181 aggatttctt gcgtgcaatc gagaaagctc gtgaagacat caaccagttt agccgtgatt
    12241 acaagggcaa tcttcacact tttgaggatt tcgagaaggc ctttgcggaa ttagaacaga
    12301 tctacattca gatgagccat attggcaact atgcttttat gcctcagacg acggactata
    12361 gcaatgacga atttgccaat attgcccaag ctgggatgga atttgaaaca gatgccagcg
    12421 tagccttgac cttctttgac gatgccttgg tggtagcaga cgaggaagtc ttggaccgtt
    12481 tgggtgaatt gcctcacttg acggcagcta ttcgtcaggc caaaatcaaa aaagcccact
    12541 atctaggggc tgatgtggag aaggccttga ccaatctcgg tgaagttttc tatagtccgc
    12601 aggacattta tactaagatg cgagctgggg attttgaaat ggctgacttt gaagcccatg
    12661 gcaagaccta taaaaacagc tttgtcacct atgagaattt ctaccaaaat cacgaggatg
    12721 ctgaggttcg tgagaaatcc ttccgttcct tctcagaggg acttcgtaag caccaaaata
    12781 cggctgcagc agcctatctg gctcaggtca agtctgaaaa actcttggct gatatgaagg
    12841 gatacgactc tgtatttgat tatcttctag ctgaacaaga agtggaccgt gtcatgtttg
    12901 accgccagat tgacctcatc atgaaggact ttgcaccagt cgctcagaga tacctcaagc
    12961 atgttgccaa ggtaaatggt cttgaaaaga tgacctttgc agactggaaa ttggacttgg
    13021 acagcgccct gaatcctgaa gtgactattg acgatgccta tgatttggtc atgaagtcgg
    13081 tagaaccttt ggggcaagaa tattgtcagg aagttgctcg ttaccaagaa gagcgctggg
    13141 tggactttgc tgctaacagt ggcaaggatt ccggtggtta tgcggcggac ccatatcgcg
    13201 tacaccctta tgtactcatg agctggacag gccgtttgag cgatgtctat accttgattc
    13261 atgaaatcgg gcattctggt caattcatct tttcagacaa tcaccaaagc tatttcaacg
    13321 cccacatgtc gacctattat gttgaagcac cgtcaacctt caatgaattg ctactcagtg
    13381 attacttaga gaaccagtct aatgacccac gtcaaaaacg cttcgctctg gctcatcgct
    13441 tgacagacac ctacttccat aactttatca cccacctctt ggaagccgcc ttccagcgta
    13501 aggtgtatac attgattgaa gaaggggaga cctttggagc aagcaaactc aacagcatta
    13561 tgaaggaagt tttgacagat ttttggggcg atgccattga aatcgacgac gatgcagctt
    13621 tgacttggat gcgccaagct cactactata tgggcttgta tagttacact tactctgctg
    13681 gtcttgtcat ctcgacagct ggttaccttc atcttaaaaa ctcagaaact ggagctgaag
    13741 actggctcaa tctcctcaaa tcaggtggta gcaagacacc acttgagtca gccatgatta
    13801 tcggtgctga tatttcaaca gacaaaccac tccgtgatac catccaattc ttgtctgaca
    13861 cagttgacca gattatcgcc tacagtgctg agttggggga gtaaggggat agagaggttg
    13921 ttatagaatg ggtatggaca gttatttgct taaaagtcca tgtttaaatc ccccattcct
    13981 tttcgaggaa ttctttgccc tgctctgtaa tggtaagtga acgtggcgtt tgtgcaggga
    14041 tgaccaattg tttttcactg aaaaattcta aaagagcatt gccgagagct ccgccgacat
    14101 gatttttacg ttcgctccaa tccaaacatt tagggcaaaa tttccgtttt ttctttttca
    14161 attcatccgt atcaatgtgt aatttttttt ggaaataaga cttcccagct tcagtgagtt
    14221 ggaagtcttt ttctgattcg ctgaggagat tttcttgcaa gagataattg gtaatggaaa
    14281 tgccaagctc accagcaata tggtcatagc aggtgcgagc gacctttaat tcctttttgt
    14341 gaatgtgttg tttataactt ttgacatcac gctgaggaga gtagaagctg agaaattcca
    14401 gcatttctgc aatggcagca ctttttaaag aataataatg aaaacgtcca taggtttgta
    14461 gagtggttac ctgcgcttcg gtaagtaatt tgaggtgata gctgacagta tgttgcttga
    14521 ttttggtaaa gctggcaatt tcgttaaccg tgtgagcttt tccatccatt agaatatcta
    14581 aaatgtgcat ccgagaaggg tctgataaga gttttgtgat gatgaaaata tctggttcca
    14641 taaaaatttt ctccaaattt catatatcga ttattttcta tcttttatta tactataatc
    14701 aaagcacaag aaaaatagat tttaaaaatg ttttactaga gaatatttta aattttattg
    14761 tctgagttta aatggaggta aaattacaaa agtaattgca gcagtatcca cagttgaagg
    14821 acgttaatct taagattaca ttctcaacat tttttcaaaa gtgagatgaa ttaacaaagt
    14881 atccaacatg ctctatttgt tggggaaaga aagagtgaag aagttaagtg ttaaaaatct
    14941 aaaaaagcaa tatggttctt atgaagctgt caagggaatt agctttgaat tgggagaagg
    15001 agagtgtttg ggtatcttgg gaatcaacgg tgctggcaaa acatccactt taaagatgat
    15061 ttactcactg acctcaattt cctcaggaga gattgatatc ttggggcaaa atattgaaac
    15121 caatcgtcgt aagagtaaat cttgtctggg tattgtaggg caggaggata tgctggatat
    15181 gaccttgaat gtttatgata atctttttgc tcacggtcta tgttatggta tctcatctgt
    15241 cgaacttagg aagagaatcg attttctctt ggattttgtt caactgagca aacatgcgca
    15301 aaagatggtg aatcagctgt cgggtgggat gcgacgacgt cttgtcttgg caagagctct
    15361 catcaatcgg cctgatatta tcattttaga tgaaccgaca gttggtttag acattcaatc
    15421 ccgcaatatt atttgggata aacttttgga gttgaaggaa ttaggtgttt ccatcattat
    15481 tacttcccac tatatgaatg agattgaata tttgacagat agggtgctga tgctccacca
    15541 agggactatc aaagaagaag gaactgtaga agacttgctc atcaagtatg atgctgacaa
    15601 ccttgagacg cttttttggg ggttgactgg gacagagaag gaggacttgc atgtttaagt
    15661 tatcatttca ctccatcgga cacgttgtgg tacgcaacta catgagtttt agaaatttat
    15721 ttaaaatttc aattgtcccc aaccttatcg accccctctt ttatctttta gcaatgggat
    15781 ttggagttgg tgcttatttg acgcatgtta acggtatgct ctaccgtgat tttgtcatta
    15841 caggtttaat tgctgcgact gccatgtctg cggccactgc agaaacaacg gtcaatgcct
    15901 ttatccagta taagattgaa aagacttatg atgctatgtt gatgacgcca atcaatacga
    15961 atgatattgt ggtgggtcag gctatctggg caggtattcg tgcagtgatt ttcggtggga
    16021 ttttttggct gatttccatg cttatcacag cgcattttca tgtcttgatg ttattgattc
    16081 ctctgatttt atttatagtg ggttatttgt ttggtgtttt gggactaatc tttacctact
    16141 tggcgccgtc tagggaattt ctgaattact acaatgtttt ggtaatccgt cctctttata
    16201 tgttttcaga taccttcttt cccattactt ccttgccccc aatacttggt gatttgactt
    16261 ggttttctcc cttgtatcac gctacccgca tgattcgtat tatatgggga ggtgaggtga
    16321 caggtttgta tcttcatctt atctggttga tagccttggc gctcttgata acggtgcttc
    16381 ctatgtatat gttacatcgt agatattata gatagtctaa ttatttgtga tgttgtagtc
    16441 atattctaat ttatactaaa cgttattcga atttggacag gtatgggaac tgtgcctatc
    16501 aatattgtaa tttatggaag ttgaaatagt acggttcagt ttttaaaata ttgttagaaa
    16561 ttgttttaaa ttccctaatc aatttgtata gtttttattt cactttatta taactagttt
    16621 ttcgcaattt ttgagacagg aatgtgattt ttgtgataag gctctatcat tacaagttca
    16681 tttgtggtca tatggaataa atcatttata tatcttttca gaaatatgct gtttctctga
    16741 atataaatgt ctttttcttt ttaacttaat tttacaaatt ggtaaagtca tttaagactg
    16801 cctttttgaa cagttgctag aaatgtagag gtattgacca tgtatcaaga tttactaaga
    16861 aaaatagcag aagaaaaacc aaattataac caggaagaaa tccagtggtt gtttgatcat
    16921 ttgggaaatc cttctccaga aattcgtgat gaccttgttt ttacaagctt tgctaaggag
    16981 attcaggaag agctatttac acaggaacaa tttcatttca ttgctgaggt ggtcttagct
    17041 gatggagggt tagataaaga gattgataag gtaggcttgt caacacttga acgttctttt
    17101 agggcactta tttatgcaaa tctcttgtct gcagatgcta accagcaatc ggttttttat
    17161 cagggattac aatcagaaat tcgtaatgtc cttttaaatc aaggcttgca ctatctttca
    17221 aaagaaaagg atacaacagg tttctcaagt cagtatggtt gggttcattc ttttgcacat
    17281 ggagccgatt tactgacaga ggtggtctgt catccagact ttcctataaa cagaattcat
    17341 gaagtatttg atatacttgg ccaactattt aaaagaatgt cgattctctt tacagatgat
    17401 gaggattggc gtttagcaag agtgatctat gaacctattt tacaagggaa gttggagcaa
    17461 gaacaagtag cttcttggat aaaaactgtt gattttccga tagaagaaag ggaggatttt
    17521 tacaaatttt ccaactttag atcctgtctg gtggaagtct atgtccaact tgaccagaga
    17581 aatagtttac aagatgacct gaaagaagct atccagtctt ttcaatacta gggattagct
    17641 aatagttcta tgattttcaa aaaatttcca atcaattttc ttgaaaccct ttcctacttt
    17701 tgatagacta gtatctagtt tttgaaaaaa ggagaaagaa aatgaaaaaa tttgttgctg
    17761 agttaatcgg tacgttcatg cttgtgttcg ttgggacagg agctgttgtt tttggaaatg
    17821 gtcttgatgg ccttggtcac cttggaatcg cctttgcctt tggtttggca atcgtggtgg
    17881 cagcctactc aatcggaact gtttcaggtg ctcacttgaa cccggctgtt tcgattgcta
    17941 tgtttgcaaa caaacgtttg tcatcttcag aacttgtaaa ctacatcctt ggtcaggttg
    18001 ttggagcttt catcgcttct ggcgcagtct tcttcctctt gggtaattca ggtatgtcaa
    18061 ctgctagtct tggtgaaaat gccttggcaa acggtgtcac tgtctttggt ggtttcttgt
    18121 ttgaagtcat cgcaactttc ttgtttgtct tgattatcat gactgtgact tcagaaagta
    18181 agggcaatgg cgcgattgct ggtttggtaa tcggtttgtc attgatggcg atgattcttg
    18241 tcggactgaa gattactgga ctttcagtaa acccagctcg tagcttggca ccagctgtct
    18301 tggtaggcgg cgcagccctt caacaagttt ggattttcat ccttgcacca atcgctggtg
    18361 gagttcttgc agcccttgtt gcaaaaaatt tccttggaac agaagaataa ttgaaactca
    18421 aaaagccttg ctcctcatct tgaggaacag ggctttttcg tatgatactc ttcgaaaatc
    18481 tcttcaaacc acgtcagctt caccttgccg taggtatggt tactgacttc gtcagtttca
    18541 tctacaacct caaaacagtg ttttaagctg acttcgtcag ttctatctgc aacctcaaaa
    18601 cagtgtttta agctgacttc gtcagttcta tctgcaacct caaaacagtg ttttaagctg
    18661 acttcgtcag ttctatctgc aacctcaaaa cagtgttttg agctgacttc gtcagttcta
    18721 tctgcaacct caaaacagtg ttttaagctg acttcgtcag ttttatctac aacctcaaac
    18781 actgctttga gcaacctgcg gctaacttcc tagtttgctc tttgattttc attgagtatg
    18841 actttagcgg ttgtcaattt tctctggata aaggtcgtgt tggaagaggc gttgttctgc
    18901 caagtcctca tacttagttc cttgcttacc gtagttgtag taggggtcga ttgaaatgcc
    18961 accgcgcgga gtgaattttc cccagacttc taaatagcga gggtctagca agttgaccaa
    19021 gtctttcccg atggtgttga tacagttttc gtggaaatct ccgtgatttc ggtagctaaa
    19081 tagatatagt ttgagggatt ttgactcgac acagagcttg tcaggaatgt aggaaatatg
    19141 aatcgtcgca aagtctggct gagcagtgat ttgtcccagc agagacatat cgaggatatg
    19201 gtgacgaatg ccctgttcct tagcgatttc tctagcaatt tgaatttcga ggtgatgacg
    19261 ttggccgtag gcaaaggtga cagcttcgac tgtttcatag tgttgcataa cccagaaaag
    19321 gcaggttgtt gaatcttgac caccactaaa gacgaccaag gctgattgac gtttcatagt
    19381 actccttcca aaatgggaaa tgttcagagc atgcaaaaag ctcctattag ggagctaaaa
    19441 aataccaaat cgagtttttt tagcgatggc atatcccaaa catcgtaata ttctacttat
    19501 atagtaaaat gaaataagaa caggacaaat cgatcaggac agttaaatcg atttctaaca
    19561 atgttttaga agtagaggtg tactattcta gtttcaatct actatagtct agcatatttt
    19621 ttgaaaaatg gcaaagggca agaaaaaaga gaccaaagaa agtacttggt ctctcgtttg
    19681 attagctcaa ttcagcaatg atggccttga tttgttctgc tgtgtgaaca cctgcaactt
    19741 gtttgacaac ttggccgtct tttttgaaga gaagagttgg aatagacatg attccaaaag
    19801 cacgagctgt gtttggattt tcatcaacgt ccattttaac gattttcaag acatcttctg
    19861 aaagttcttc agacaatttg tccaagattg gaccttgcat acgacatgga ccacaccaag
    19921 ttgcccagaa gtctactaag accaaaccgt cttttgtttc ttgttcgaat gttgcatctg
    19981 taattgcttt tgccattgta tttctccttt ttttagttat attggcttaa atcttgtttc
    20041 atgagataga agaagatatc tccataagtc ccatggtagt ccaaattatg acccttgtaa
    20101 gttaattttt ggacagggta gtagtctgcg acgccgataa ggcaagcttg ttgcgaacgt
    20161 tcaaagtctt cataagactc gaaagtcaca gttctttcgt tcttgctggc atctatatag
    20221 gtaatttcaa tcatatttaa aactcctttg tttaatgcta actttatttt actccttata
    20281 aaagagaatg tcaagaaaaa tgattgcgca cgcaactttt tctaaaatca tcttaaatca
    20341 agaaatccaa acctgcttcc aagctttctt cgacagtctt ttgtagcgag gccagtatct
    20401 tttgcccatc atttgtcagg cagataaaac tagagcgtct atcttgatgg caacacatgc
    20461 gactgagtag accgcaattt ttagcttcca agcgagccac catcctagaa actgcgctcg
    20521 ggctcagatg aagcttatct ggcaggtcaa tctggcgtag agatttttct tcagccaagt
    20581 ccagatagta gagcaggtag aactctttca aggtcagact ttgctcgctc tgttgggcaa
    20641 cggtctcttc caagagactt tcaatttctt tctgacgccg attgaagtca aaccattttt
    20701 ccaaataggt catagtgtct cctttctttt tagagtcata aatagaagaa agtccattaa
    20761 cgggcagtct ctgcgtcaca agatgattgc gcatgcaata attatactac ttttcaagaa
    20821 tgctggcaag ctctgttttt tagtggtttt attttttact gtctatattt ttggtaaaaa
    20881 tcaactttta cttggatgaa ggttttggct tcacgtagga gttgaagaag ggtggcgcgg
    20941 gtttcaaatt cttctcttgt cttgggcaga ctgcggttcc ggaagacttc cagataacgt
    21001 tcaatttcat ctagcaaatc agaagcagga ttggtctggc tcagttgacc tgcaattttt
    21061 gaaaagagtt gcgctaagat caggctttca ctggcggcaa ggtgacaagt gttaatctgt
    21121 tgggccatgt ttctcaggat acgactttgt cgctgtctca tctcaaagta gtggatatgg
    21181 tagtcggtct ggtgaaagag gtggtcagag tgatccaaat agaccagtct gagggcttct
    21241 ttcaaaagcg tgtctaattc tgctaccagc tgtgctcggt tgcgtccgtc tcctctggat
    21301 aaatagtatt tgaagcgctg gaggatatct tttaactttt cttccaccag cgtgtggtag
    21361 tgctggattt cctcttctcg tgaaggcata tagagattaa caagcaaggc aaatcctgta
    21421 ccaatagcaa agagaaggaa ttcattgact agaaggtctg gagaggttga ctcttgaacc
    21481 aagagatggc taaccaaaac agtgcttggt gtgatgccaa tttcccagcc catcttgtag
    21541 gctaaaggaa cgtagaaggc cagatagagg ccgagactcc agatatgaaa tccgctcaag
    21601 tgaaaagcta gaacaccgat agccagagct agaagcatag aaaaaagacg attgcgagcc
    21661 agttttaaag tacttctacg cgtatcagat aggctcaaga gagcgataat tccagccgaa
    21721 actgctgacg aaagattgag aaaataagca agcaggcagg caagacaggt agctaagatg
    21781 agcttggtcg tacgttggct aatagacata agaatttcct aataagttag aataaaagcg
    21841 taaaagacaa gacatgagca ggcttgcctt gatgagttat tttttacggg ttgctgcgta
    21901 ttcggcaacg gcggtaaaga ggacatctgt agaagagtta agggctgttt cacatgagtc
    21961 ttggatgaca ccaatcacaa aaccaacccc aacaatttgt atggcaatat cgttagaaat
    22021 accgaaaagg ctacaagcaa ctgggataag aaggagggaa cctccggcaa tacctgaagc
    22081 atcacaggat gagatagctg ctaccacact gaggacaaag gctgtggcaa agtcaacagg
    22141 aattccaaga gtgttaactg cagcaagggt caaaaggtta atggtaatcg ctactccagc
    22201 catattgata gtagaaccga gtgggataga aacagaatag gtatctgggt tgagtccaag
    22261 gttatggcag agtttcatgt tgacaggaat gttagtcgca gaactacgag tgaaaaaggc
    22321 tgtcacaccg ctgacacgga ggcagttcca aactagagag taaggattgc gtctcataaa
    22381 gaagaaggca atcaaagggt tgaccacagg ggcaacaaaa agcatagtcg ttactaatgg
    22441 aaccaataaa ataccgtagt tggcaaggct tccgactccc ttgtcagaaa tggctttaaa
    22501 aacaagacca aggattccaa atggagccag attgatgatc cattcgacaa ttttagaagt
    22561 cacgtcagcg atagttttta gcaattcttt actattttta ctagcttctc tcatagcgat
    22621 tccaaaaatg actgcccaag ataagattcc aatatagtta gcagtaagca gggcgttgac
    22681 tgggctgtca accagtttga gcaagaggtt gctgaggacc tgcccaatcc catctggtgg
    22741 tgcaatttca gtattggcac tatttagggt aatttcaata gggacgatga aacttgctag
    22801 tacagctaca agagcagcgg cgaaagtccc tatcatagga tatacaagaa aacaacagtt
    22861 ttcatattgc tatcttgtcc cttttgatgt tgggaaaggg cattggcaac gagggcaaag
    22921 actaggatag gagcaacagc tttcagacct ccaacgaata aatccccgag tagcccaatc
    22981 cctgagagat taggaagggt cagtcctagg attccccaca aagcatacca atcaagatac
    23041 gcttgacaag gcttgcctta ttccaagcat gaatgattct tttcataata atctcctttt
    23101 tgtgtagtga ttatgattat agtataaatg atagacaaaa tcaagaattt tctgtctatt
    23161 ttttgaatat ttatggagaa tgagactgat gaaaatatgg tataatgaaa taaaggagtt
    23221 ttatatgcaa aaatttattc aggcttatat tgaaaagcta gatgtgacaa ccattatcga
    23281 gaatattcta accaaggtca tttctctttt actgcttttg attgtatttt atattgctaa
    23341 aaaaatgctt cataccatgg tgcagagaat tgtcaaacct tctctaaaaa tgtctcgtca
    23401 tgatgtcgga cgccaaaaaa ccatctcacg tttactagaa aatgtgttta attatacgct
    23461 atatttcttt ttactctact gcattttgtc gattttaggt ttgccagttt ctagtttgct
    23521 ggctggagct ggtattgctg gggtagcgat tggtatggga gcccaaggct ttctgtctga
    23581 tgtcatcaat ggctttttca tcctctttga acgtcaactg gatgtgggag atgaggtcgt
    23641 tctgacaaat ggaccgatta ctgtatcggg taaggttgtc agtgtgggaa ttcgtacgac
    23701 acagcttcgt agcgaggagc aagcccttca ctttgtccct aaccgaaata tcacagttgt
    23761 tagcaatttc tcacgcacag actagacctg ttattttaag taatttgtgg tacaatagag
    23821 ggagtttaat aaggagaaaa gatggtttta gaaaagcagt tgggcaatgg ttgtacctgg
    23881 atagacctag acctaggaaa gttgaataaa ctagaagacc tttctgaaat ttacggtttg
    23941 gacaaggaaa ccattgaata cgcactggat agaaacgagc gcgcccacat ggactaccac
    24001 cgtgaaagtg ggacggttac ctttatctat aatgtcctag acgtaaaaaa ggacaaggcc
    24061 tactatgaga cttttcccat gacctttatt gtcgagcatc gtcgcctgat taccattagt
    24121 aataccaaga acgcctatgt cattgaacag atgactcgtt atctggagaa ccatgacacg
    24181 ctttcgattt ataagtttct ctttgccagt ctggaaatca tcagcaatgc ctactatcct
    24241 gtcattgagc agatggacaa gagtagggat gaggtcaatg acctcttgcg ccagcgaact
    24301 accaagaaaa acctctttgc cctgtctgat ttggagactg gtatggttta tctgacggca
    24361 gctgccaaac aaaatcggat tttgttagag catattcaag gtcatgcctt gtatcgtagt
    24421 tttgatgaga ttgagagaga acagtttgat gatgccatga ttgaggctca tcagctggta
    24481 tccatgacag acctaatctc tcagatttta cagcagcttt cagcctctta caacaatatt
    24541 ctaaacaata atctgaatga caatttgaca accttgacta tcatttcagt cttgctagct
    24601 gttttggcag tcgtgacagg ctttttcgga atgaatgttc ccttaccttt aacagataag
    24661 ccccatgctt ggctctatat cagtttggct agtgcaggtt tgtggattgt tttatccttg
    24721 ttactaagga aaattgcgaa aaaaagttaa gaaaaggagc cagaatggcg attgaaaatt
    24781 atataccaga ttttgctgtg gaagcagtct atgatctgac agtcccaagc ctgcaggcgc
    24841 agggaatcaa ggctgttttg gtcgatttgg ataataccct cattgcttgg aacaaccctg
    24901 atggaacgcc agagatgaag caatggctac atgaccttcg ggacgcgggt attggcatta
    24961 tcgtagtgtc aaataacacc aaaaaacgcg ttcaacgagc agttgagaaa tttgggattg
    25021 attacgttta ctgggccttg aagcccttca catttggaat tgaccgtgcc atgaaggaat
    25081 tccactatga caaaaaggaa gtggtcatgg ttggtgacca gctcatgaca gatatacgag
    25141 cagcccaccg tgcagggatt cggtcaattt tagtcaaacc cttggtccaa catgactcaa
    25201 tcaaaacgca gattaaccga actcgtgagc gtcgtgttat gagaaaaatc actgaaaagt
    25261 acggaccgat tacatataaa aaaggaattt aactatggaa gaaattctct gtattggttg
    25321 tggagcaacc attcagacga cagataaagc tggtcttggt tttacccccc agtcggcact
    25381 tgaaaaaggt ttggagactg gcgaagtcta ttgccaacgc tgtttccgtc tccgccacta
    25441 caatgaaatc acagatgtcc agttgacgaa cgatgatttc ctcaagctct tgcacgaggt
    25501 gggagacagt gatgctttag tggtcaatgt cattgatatc tttgatttta atggatctgt
    25561 catcccaggt ttaccacgtt tcgtctcggg caatgatgtc ctcttggtag gaaataaaaa
    25621 agatatcctt cctaagtcag ttaagtctgg taagattagc cagtggctca tgaaacgtgc
    25681 ccatgaagaa ggtcttcgtc cagtcgatgt ggtcctaact tcagcacaaa ataaacatgc
    25741 cattaaggaa gtcattgaca agattgaaca ctaccgtaag ggccgcgatg tctatgtggt
    25801 cggtgtgacc aacgttggaa aatcaactct aatcaatgct attatccaag aaatcacggg
    25861 tgatcagaat gtcatcacta cttcacgctt cccagggaca accttggaca aaatagagat
    25921 tccgcttgac gacggatctt atatttacga tacgccggga attatccacc gccaccagat
    25981 ggctcactat ttgacggcca aaaacctcaa gtatgtcagt cctaaaaagg aaatcaagcc
    26041 taagacctat cagcttaatc ctgagcaaac cctattttta ggtggtttgg gacgctttga
    26101 ctttataaca ggagaaaagc aaggatttac tgctttcttt gacaatgaac tcaaactcca
    26161 tcgtagcaag cttgaaggag ctagtgcttt ctacgataag cacctgggaa ctcttctgac
    26221 accaccaaat agcaaggaaa aagaagattt cccaaggcta gtccagcatg tctttaccat
    26281 taaagataag acagacctag tcatctcagg cctaggctgg attcgtgtaa caggcacagc
    26341 aaaagtcgcc gtctgggcac cagaaggcgt cgccgtcgtc acacgaaaag caattattta
    26401 aaacaaaatc gctgagcgaa caaagtgagc tcatatagcg atagttattg ccgtggtgtg
    26461 attgccaatc tgtagattgg caaggggcac aattgagacc aaggctcaat tgtgaggtca
    26521 ggaaatccat ttttcaaaga tgaaatcttt ggaaaattag ttccgaccgt ccctcaccac
    26581 ctaaaataac tatcaaaaaa caaggaagaa aattatgtca ttaacatcaa aacaacgtgc
    26641 cttcctcaac agccaggcac acaccctcaa acctatcatc caaatcggga aaaatggact
    26701 caacgaccaa atcaaaacca gcgtccgtca agctcttgat gcgcgtgaat taatcaaggt
    26761 tactctctta caaaacacag atgaaaacat ccacgaagta gctgaaattt tggaagaaga
    26821 aatcggtgtg gatacagtcc aaaaaatagg acgcatcttg attttgttta aacaatctag
    26881 caagaaagaa aatcgcaaga tttctaagaa agtcaaagaa atctaagacc gaaactccaa
    26941 ataactgttt tcatagagaa ataaagggga ctagcctatg acaatcgaac tattgactcc
    27001 ctttaccaag gtagagttgg agccagaaat caaggagaaa aaacgcaaac aagttgggat
    27061 tttagggggg aattttaacc ctgttcacaa tgcccatctt attgttgcgg atcaagtacg
    27121 gcaacagttg ggactggatc aagttctgct catgcctgaa taccaacctc ctcacgttga
    27181 taaaaaggaa accatccctg aacaccatcg tctcaagatg cttgagttgg caattgaggg
    27241 aattgacggc ctagtcattg aaaccattga gttggagcgc aagggtattt cctacaccta
    27301 cgacaccatg aagattttga cagagaagaa tccagatacg gattattact ttatcatcgg
    27361 tgccgacatg gttgactatc tgcctaagtg gtaccgaatt gatgaactgg ttgacatggt
    27421 tcagtttgtg ggggttcagc gtccacgcta caaggtaggg acttcctatc cagttatctg
    27481 ggtggacgta ccgctcatgg atatctcgtc cagcatggtg cgggacttcc ttgcccaagg
    27541 tcggaaaccc aactttctcc tacctcagcc agtgctagac tacatcgaga aggaggggct
    27601 ctactgatgt cctatcaaga ctatatcaac tgctcccgtg aggccttgtt ggaaaaaatg
    27661 gcagagcttc tacctgagaa acgtctaacc cattgtctag gtgtggagcg tgccgctatg
    27721 gaattagctc agcgctttgg tgtagatgtt gagaaagcaa gtctagcagg tcttcttcat
    27781 gactatgcta aaaagttgtc agatcaggaa tttctagcct tgattgaccg ttaccagcta
    27841 gaccctgacc tcaaaaactg gggcaacaat gtctggcatg gtatggtcgg aatttacaaa
    27901 attcaggaag atttggattt gcatgattct gaaattctgc gagccattga aattcataca
    27961 gtcggagctg gtcaaatgac agacctagat aaggtcatct acgtcgcaga ctatatcgag
    28021 cacaatcggg catttcctgg agtggatgtg gcgcgtgaga ttgcaagtct atcgctcaat
    28081 aaggcggtgg cctacgaaac agctcgtacc gtggagtatc tagctcatca gggattcccc
    28141 atctatcccc aaacccttga aacctataac gcctttgtgc actatttgaa agaggactaa
    28201 atgaagacgg cttttataat cattgatgtt cagaatgttt tagtagaaac tgggttccag
    28261 acaaaaagtc tattggaaaa aatttcttat ttacaaaacc aggctagaag caagaatatt
    28321 gaaattatct atgttcaaca tattgagaac tctgaagctc aaacatcaga agattggcag
    28381 ttatctgcgc ttttaaatcg aaaacctgct gaaaaggtct ttcagaagaa gtataacagt
    28441 attttcaaag aaactggctt aaaagaatac ttggataagc aggggattga aaaattagtt
    28501 ttatgtggta tgcagacaga atattgtgtg gatacctctg tcaaggttgc ctttgaatat
    28561 ggctatcagc ttattgttcc agaaggtgct gtcacaacct ttgatgggga tgacattcca
    28621 gcagaaacga ttaatgaatt ttatgaggac atttgggagg agcgctttgc agatgttcta
    28681 gattacaaac atattttcta aaaagaggat taaatgaacg aaaaagaatt actagaacta
    28741 gtcgtgaaag cggctgatga gaaacgtgcg gaggatatcc tcgcacttga tgtacaagat
    28801 ttgactagtg tgacggacta ctttgtcatc actagctcaa tgaatagccg tcagttggac
    28861 gctatcgcag ctaatatccg tgaaaaagta gctcaagcag gctttaaagg tagccatgtc
    28921 gaaggcgatg cagctggagg ctgggtctta ctggacctcg gtgctgttgt cgtgcatatc
    28981 ttttcagaag aaatgcgtgc ccattataat ctagagaagc tatggcatga ggcgaattca
    29041 gtagatattt cagaagctct tgcttagaag atgaactcag ttgtagtcaa ctgggtttct
    29101 ttgcttattt tagaaaaatt gatagaaagg taaagggcct cgtgtttgtt tcatttcgaa
    29161 actgaacacg agctaaaagc tgagataaaa agtaactttc tttgtatctg acgataggga
    29221 agaaagtttc ctatgtactc tttgctttta aggctctttg tatcttagaa aggatttgaa
    29281 cccgtcctgt aattctcgga aaatagatag ccttccttgg agctcggctc catcgtaagg
    29341 ctcctaattt tctatcgaat tacgggcgga cttggtatca tgttttatgg cgacttatga
    29401 aacctttgcg gcggtctatg atgcggtcat ggacgatagt ttatacgaca aatggacgaa
    29461 tttttctctg cgtcatttgc ctaagaccaa ggagagaaag aaactcttgg aattggcttg
    29521 tgggacagga attcaatcag tgcgcttctc tcaggctggt tttgatgtga ctggacttga
    29581 cttgagtgcg gatatgttga agattgcgga gaagagagca acttcagcca agcaaaagat
    29641 tgcttttata gaaggtaata tgctgaattt gtccaaggca gggaaatacg attttgtcac
    29701 gtgttattcg gactctatct gctatatgca ggatgaggtg gaagtagggg acgtctttaa
    29761 ggacgtgtac aatgcgctga atgaagaggg agttttcatc tttgacgtgc actcgaccta
    29821 ccagacagat gaagtattcc ctggctattc ctaccatgaa aatgcggaag attttgccat
    29881 gctttgggat acatatgagg acgaagctcc tcactccatc gtgcatgagc tgactttctt
    29941 tatcaaggag gctgacggtt cctttagtcg ccacgatgaa gtacatgagg agaggaccta
    30001 tgagatcttg acctatgata tcttgctgga acaggctgga tttaagtcct tcaaactcta
    30061 tgcagacttt gaggataagg agccaacaga aactagcacc cgttggtttt ttgtggcgca
    30121 gaagtaggag aacatcatga ccatcacagg tattatcgca gagttcaatc cttttcataa
    30181 tggccataaa tacctgctgg atcaggcaga gggactgaaa attgttgcca tgtcagggaa
    30241 tttcatgcag cgtggagagc ctgctattgt ggataagtgg acaagggccc agatggcgct
    30301 ggaaaatggc gcagacttgg tagtggaatt gcccttttta gtcagtgttc aggcagctga
    30361 tttctttggt caaggagctg tggatatctt ggatcgcttg ggcattgata gtctagtttt
    30421 tgggacagag gaagttcgag attatcagaa aatcgctgac ttatacacag agaaaggtac
    30481 tgagatggag aaatttgtgg aaaatctgcc tgattccctt tcctatcccc agaaaaccca
    30541 agctatgtgg aaggaatttg ctggtcttga tttttcaggt aataccccca atcatgttct
    30601 tgctctggcc tatgccaagg cggttgcagg acggaatatc aagctgcatc cgattcagcg
    30661 tcagggggca ggttaccatt ctgtgaataa agatgtggac tttgcctcgg cgacagccct
    30721 ccgtcaacat cagaaggacc aagatttctt agaacgtttt atgccttctg ttgccctatt
    30781 tgagcaggcc agtaaggtga tctgggaaga ctattttccc ttgctccgct atcaaatctt
    30841 gtcaaatccc gacctaacca ccatctatca ggtcaatcaa gaaatggctg tgcgcattaa
    30901 agaagctatt aaaacagccc agtctgtgga agaattggtc gagttagtga ctaccaaacg
    30961 ttacaccaag gcgcgtgtca gacgcctctt gtcctatatc ttggcgcagg ctagagaaaa
    31021 tgtcttgcca gaagccattc atgtccttgg atttactgaa aaaggcaggc aacatctcaa
    31081 gtctttgaaa ggtcaggtca gtctagtcag ccgaattggc aaagaacctt gggatgctat
    31141 gacccaaaaa gcagaccaga tttaccaact aggaaatcta agtatagcac aacagaattt
    31201 tggaagagtg ccgattagaa tagaaacaaa ctaagtctac cgaaaggtgg actttttgag
    31261 attttttacg aataatactc ttcgaaaatc aaattcaaat cgcgtcaacg tcgccttgcc
    31321 gtactcaagt acagcctgcg gctagtttcc tagtttgctc tttgattttc actttaaaca
    31381 tgatttttcg cgtcttcctg agattatgtc ttgggatgag tatgctttta caaagggaaa
    31441 gatgagcttc attgcgcaag attttaacaa tctcaatatc atcactgttc ttgagggaag
    31501 aaaacaagct atcattcgaa atcactttct tcgctacgat agagctgtcc gatgtcgcgt
    31561 caaaattatt actatggata tgtttagccc ttactatgac ttggctaaac agcttcgctt
    31621 tcaaatttct aggctcaggc tgaaacagtc tcccagactg tttcactccc gaatgctaaa
    31681 atcgttcttg atcgctttca cattgtgcaa catcttagcc gtgctatgag tcgtgtgcgt
    31741 gttcaaatca tgaatcaatt ggatcgaaag ttccatgaat acaaggctat caagcgctac
    31801 tggaagctca tacaacagga tagccgtaaa ctgagtgata agcgatttta tcgtcctact
    31861 tttcgtatgc atttaaccaa taaagagatt ttagacaaac ttttgagcta ttcacaagac
    31921 ttgaaacatc actatcagct ctatcagctc ttgctttttc acttccagaa taaggaacca
    31981 gagaaatttt tcggacttat cgaggacaat cttaagcagg ttcatcctat ttttcagact
    32041 gtctttaaaa ccttcctcaa agataaagaa aagattatca acgcccttca actacactat
    32101 tcaaacgcca aactggaagc gaccaataat ctcatcaaac ttatcaagcg caatgccttt
    32161 ggttttcgaa actttgaaaa cttcaaaaaa cggattttta tcgctttgaa catcaaaaaa
    32221 gaaaggacga aatttgtcct ttctcgagct tagctgactt caacccacta cagttgacaa
    32281 agcgcctatt ttgtggtata atgaagagaa ttgtaaatca taaaaacagg ggaagcaaat
    32341 gtctaatttt aaagaaaaac taccgatatt ccagtcgaac gatgttcaga tttcaaatgt
    32401 agtcaatgca gtttggtcga ttgcgaacac cttacgtgga gcttatcgtt cggataagta
    32461 tcgagtgatg taattaaaaa ttggatgaga agaaaagata caattgagtt tcttggttta
    32521 tggaaaagct taaataacat gaatttcaat tcggtcgaat tcgaccgaat taaatcggaa
    32581 gctggatata attcatttac attgtctcct aagaaatggg tagagaaaac tggagcaatc
    32641 ggaattatct caaaaggtgg aagatatggt ggtgcatttg cacatacaga tattgccttt
    32701 gaatttgctt tatgtatttc tgctgaattt aagatgtatg taattcagga ttacaaaaga
    32761 cttaagtcag attaaaattc taagctatca ttaggctgga atttgaatag agaaatttct
    32821 aagattaact acaaaattca tacagatgca atcaaagaat atctcttaaa agatcttacc
    32881 aacgaacagt tgtcttacaa gtatgtaagt gaagcagata tgatcaatgt tgttttattt
    32941 aacaaaagag ctaaacagtg gcgtgaagaa aataggaaca attagtacaa cacttgtagt
    33001 agttgcggga gtaagtgcgg gtgtgcttgg aggaaaattt gactttaaat ttcctaaaaa
    33061 gatttaatgg agaataataa atatgaagtt taaaaattat ctatttgatt tatatcctta
    33121 ttttcccaaa ggtttttcat tagataatag agaggatcct gatgtatgta gtaaggcgtt
    33181 gtacgatgat ttatgtaaga tgttttttga tgatgattca aaagaaaaac ttaaaataac
    33241 atctgtatgt aataaatgcc aaaactatgg aaatagagat tattatactt tatttattga
    33301 taaggataag tatttattat caagtgatta tataggagcc tctatttatt gggcacaaga
    33361 ggcaggatta aacgacagaa taattttaga tcatttgagt atttcaagaa caataggtgg
    33421 acacattctt ttccctagag gtggaaaatt agaaaccgtt aatcaggcta gaggtggaga
    33481 aaaaggatat tatgatcgtt ttgatttaac actatatgct ataaaagaat ggtttgtgga
    33541 aaataaaaat acaaaaatag gttatgctat agagaattac catgaatggt ttgaactatt
    33601 ttctggtgat gataattgta aaaatggatt tgagaacttt gtcgagtttt tcaagttaga
    33661 aggatttata tatgagcaaa ataaaattat agatttaatt aaatcagatt tagagaacaa
    33721 tcaggttgtc tttttagata aagaagatat tttgattgct ag
//
LOCUS       DAVKFH010000021        33127 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKFH010000021 DAVKFH010000000
VERSION     DAVKFH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.021"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..428)
                     /locus_tag="VW006_001040"
     CDS             complement(<1..428)
                     /locus_tag="VW006_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379908.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILT"
     gene            complement(425..757)
                     /locus_tag="VW006_001041"
     CDS             complement(425..757)
                     /locus_tag="VW006_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9379909.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(813..1715)
                     /locus_tag="VW006_001042"
     CDS             complement(813..1715)
                     /locus_tag="VW006_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379910.1"
                     /translation="MMRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(1721..2038)
                     /locus_tag="VW006_001043"
     CDS             complement(1721..2038)
                     /locus_tag="VW006_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379911.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGDWNTFKNNIKGLARASSGFRF
                     AARETGLWAAIWSTRVYSYTYVVSAFAPILGSVAAVNAALAVIGGISVAGITYALWTR
                     KFY"
     gene            complement(2183..3535)
                     /locus_tag="VW006_001044"
     CDS             complement(2183..3535)
                     /locus_tag="VW006_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU9379912.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADNKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLLPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(3532..3999)
                     /locus_tag="VW006_001045"
     CDS             complement(3532..3999)
                     /locus_tag="VW006_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9379913.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(3992..6211)
                     /locus_tag="VW006_001046"
     CDS             complement(3992..6211)
                     /locus_tag="VW006_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9379914.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIMEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
     gene            6677..7540
                     /locus_tag="VW006_001047"
     CDS             6677..7540
                     /locus_tag="VW006_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9379915.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(7829..9220)
                     /gene="argH"
                     /locus_tag="VW006_001048"
     CDS             complement(7829..9220)
                     /gene="argH"
                     /locus_tag="VW006_001048"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9379916.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDVIGSLAH
                     VQMLGQTGILSLEESEKIQVGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGYVLDKLAHLKGVLLDLAENHVATIMPGYTHL
                     QHAQPISFAYHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKQPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TTGSSIMPQKKNPDMAELIRGKTGRVYGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(9249..10446)
                     /locus_tag="VW006_001049"
                     /pseudo
     CDS             complement(9249..10446)
                     /locus_tag="VW006_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            complement(10463..11269)
                     /locus_tag="VW006_001050"
     CDS             complement(10463..11269)
                     /locus_tag="VW006_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9379917.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLVTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYSVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(11476..12117)
                     /locus_tag="VW006_001051"
     CDS             complement(11476..12117)
                     /locus_tag="VW006_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9379918.1"
                     /translation="MIELENITKTIGGKVILDRLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(12114..14222)
                     /locus_tag="VW006_001052"
     CDS             complement(12114..14222)
                     /locus_tag="VW006_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9379919.1"
                     /translation="MKKISHLCMLLLLLCTTFFVLNVNVTREIVRIQEMGKTTYSFDL
                     YLKDVTKPTETILQFFEEVASQDKVSIIKVDNGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLFSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     ALASRMEKRSLVELLKGGIL"
     gene            complement(14277..14570)
                     /locus_tag="VW006_001053"
     CDS             complement(14277..14570)
                     /locus_tag="VW006_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9379920.1"
                     /translation="MIGEINHEKISVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            14976..15296
                     /locus_tag="VW006_001054"
     CDS             14976..15296
                     /locus_tag="VW006_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9379921.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(15348..15935)
                     /locus_tag="VW006_001055"
     CDS             complement(15348..15935)
                     /locus_tag="VW006_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9379922.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            16177..16848
                     /gene="sdaAB"
                     /locus_tag="VW006_001056"
     CDS             16177..16848
                     /gene="sdaAB"
                     /locus_tag="VW006_001056"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9379923.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            16857..17729
                     /gene="sdaAA"
                     /locus_tag="VW006_001057"
     CDS             16857..17729
                     /gene="sdaAA"
                     /locus_tag="VW006_001057"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9379924.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            17751..18383
                     /locus_tag="VW006_001058"
     CDS             17751..18383
                     /locus_tag="VW006_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9379925.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            18409..18507
                     /locus_tag="VW006_001059"
                     /pseudo
     CDS             18409..18507
                     /locus_tag="VW006_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(18689..20539)
                     /locus_tag="VW006_001060"
     CDS             complement(18689..20539)
                     /locus_tag="VW006_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9379926.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(20581..21738)
                     /locus_tag="VW006_001061"
     CDS             complement(20581..21738)
                     /locus_tag="VW006_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9379927.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENVEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            21796..22962
                     /locus_tag="VW006_001062"
     CDS             21796..22962
                     /locus_tag="VW006_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9379928.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            23101..23427
                     /locus_tag="VW006_001063"
     CDS             23101..23427
                     /locus_tag="VW006_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9379929.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            23414..24007
                     /locus_tag="VW006_001064"
     CDS             23414..24007
                     /locus_tag="VW006_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9379930.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            24000..24911
                     /locus_tag="VW006_001065"
     CDS             24000..24911
                     /locus_tag="VW006_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9379931.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            24974..26038
                     /locus_tag="VW006_001066"
     CDS             24974..26038
                     /locus_tag="VW006_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379932.1"
                     /translation="MKQEWFESNNFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(26110..26970)
                     /locus_tag="VW006_001067"
     CDS             complement(26110..26970)
                     /locus_tag="VW006_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9379933.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDDSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLAQEK"
     gene            complement(27092..28078)
                     /locus_tag="VW006_001068"
     CDS             complement(27092..28078)
                     /locus_tag="VW006_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9379934.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(28350..29825)
                     /locus_tag="VW006_001069"
     CDS             complement(28350..29825)
                     /locus_tag="VW006_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9379935.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(30083..31006)
                     /locus_tag="VW006_001070"
     CDS             complement(30083..31006)
                     /locus_tag="VW006_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9379936.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(31020..31949)
                     /locus_tag="VW006_001071"
     CDS             complement(31020..31949)
                     /locus_tag="VW006_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9379937.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(32268..32489)
                     /locus_tag="VW006_001072"
     CDS             complement(32268..32489)
                     /locus_tag="VW006_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379938.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(32443..32703)
                     /locus_tag="VW006_001073"
     CDS             complement(32443..32703)
                     /locus_tag="VW006_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379939.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT        10194 a         7026 c         5694 g        10213 t
ORIGIN      
        1 atagttaata taaaactaga aatctctaaa tattttatcc aaggagattg agacaccttg
       61 cttctaaact gactaatttt cttatattcg ttagtcaatt tagtagcaaa atcttcccca
      121 attaaatgaa agattgctaa ctctaggata aaaacactta ttaatacggt ataagtcaaa
      181 aaactttctg ggaaaaactt attgattaaa atggggaaac tcattaacag aatcgaattt
      241 atggctagac agatagatac tattttattt ttcacagaat atagaggtag tgatactatt
      301 gagtagataa aagctaaaaa aataagcaaa aaatactccg tgtcatttat aatcgccctg
      361 aatgataaaa atatatacat ccaacatacg ataatatgta tttttcgaat cttatctaca
      421 ttattcatct tactgtcgca gatataaatc catcccaagg ataagggttc ccgctaatat
      481 cacaacctct attaagagca ccatatccaa tacagtaagc tatttttcct gctgctgcac
      541 caataagcca gcctccaaga cctccaacct taatatagtt tattagtttt ttggttaaaa
      601 ctgcagctcc agctaatgct ataaaagttc ctgaactcat agctaaagct acgcaaattc
      661 cttgacaagc acgcttgctt aaataagctc ccccatcaag atacatcatt tcttcttcat
      721 caatcacaac ataattattt ggtaatacaa gttccatttg ttttctcctc cttagttgaa
      781 attgtttcat ttgttttaca aataggacga acttaaacta tctttttatt actcagtaat
      841 aatttccgag atttcatttt atgatactct ctattcatac agaaatcaaa attttgcctt
      901 aaccaacttg tataatagaa aaaatctaat tgatttattc tgatgagctg tattctaatt
      961 ttatcaacct tttcttgcca gttgttattg ttactaatag aaatctcata cagcagtaac
     1021 tcgacaagac ctgtacacag aagggtgtta tgagaatctt caattttact cagaatatcc
     1081 tcaaaaagtt cactatccgt taaaaaggtg gattgattca tcgcatcata cagctgaaga
     1141 gacattaagt cagaataact gagatctgat ttttgtattt tatcctttaa cgtatttgtc
     1201 aaatcacttt caaacgaatc ttctttttcc catttatttt gattgaaaag aaattccgtc
     1261 ttattaaaaa cgagatgatt gatatccatt atagtccaaa aaaatattgt agtaagcata
     1321 atgatgacaa aaggaatttt taaaataaat actaggataa tagcaatttc aagaataaaa
     1381 ctccatataa tcctagatct taaatcatca ggcgacaaac ttttacgatg gaatgaaaaa
     1441 tagggcgaga acctcacatt tactttcttc ctgcctataa aaataaaagg aaaaatcgta
     1501 ataagagctt ctttcttgta caaatctttc aatgcgtata gtatgtctaa aactattttt
     1561 gataaaatca aactacagaa agcaagactc atactaatca caaaatcaag gggagattct
     1621 agattcaata gtaagttatt cactaggaca tgcaactcta gccacaagac aaaaagaggg
     1681 agcattagta agaaaacaat tagtttacgc attataacaa ttagtaaaat ttacgcgtcc
     1741 ataaagcata cgtaattcct gcaactgaaa tacctccaat tacagccaat gccgcattaa
     1801 cagctgctac tgaccctagt attggagcga aagcagaaac aacataagta taagaataca
     1861 ctcttgtaga ccatattgca gcccataaac cagtttctct tgcggcaaac ctaaatcctg
     1921 aggaagctct cgctaaacct tttatattat ttttaaatgt gttccagtct cccccatcaa
     1981 ggtacatcat ctcttcttca tcaatcacaa cataattatt tggtaataca agttccattt
     2041 gttttctcct ttttattaag catggttagg atggtttcct aacaagtaat cgtcgtctgt
     2101 gtttctatca cttagctacc tagataagat atcctttctc taccttattt gtttttatag
     2161 gttacctaag gtttaaaacc aattattgct tgaaacttaa catttctaaa atccaatcaa
     2221 gataggtttc tttatcatag acaatccgca cttcaactgg catatccttt tggaggacga
     2281 ctgtctcact tccatgtttt agagttaagg tttctaattc tatcatgact ttataaagac
     2341 taatattgcc ttctttcgtt tcttgggaaa ttgttcctga atcaatctgt ctgacttgtc
     2401 cctttagtgt tccatatttt tgactattta caccagtaat agcaacatca gcttttgctc
     2461 ctttcgaaac acgagaaata tcactcgcaa gtacaaaagc ttctacatag taaccttttt
     2521 ctttccctga aacttctgct atcgtttgcc cctgctgaat agacattcct tgtttcaaag
     2581 gtaacagata atgcagagta ccttcatttg gcgctaatat actatgagct ttatcttgac
     2641 ctgttgccac tccaagatta gcctcaagct ctgtaatact tttattgttg tttgaacgag
     2701 cagtacctaa ctcactaact aattgagcat aaatattata agcctgagaa gttggatcat
     2761 tataggtacg gttcatatcc ttaatattgc tttgttgact atcaatctcg ctattcttag
     2821 aagtaatatt tgtttttaat gcttcgattt ttgttgttat ttctgatatc ttagtttcta
     2881 acttagcttt gttatcagct gatgtcgtat ctgatagaga aggtgagtct ccttgctgtt
     2941 gaagttgaag caattcattt tgtagagctt gcaagtcggc atctaactga tttctttcag
     3001 ctgttatttt attcaacttc gtatattcat cctgaatctt tgaatactgg gtaccattat
     3061 tataattttc cgaatttaac tgagataggt agtattcaac tttcccataa tattcctgtt
     3121 cttcaccaga attagataaa tgattgtatt tctcatttaa tgattgttca aatttttgaa
     3181 agatagcttc ttttgcacgt aacttctcca attgtttatt taaagagctc gcttgtaaac
     3241 cttcttgccc acttgataac gaaataatga catctccagc atttaccata tctccttctg
     3301 acttatgtaa agtaactacc ttccctgaac caattgctga taggaactct gtacctgtta
     3361 taactgaatt tccattcgct tttacaatat agtttttggg tatataagct gcgccaacca
     3421 atgcaccgct taagataata gcagttgaaa taatgagaat aaacgcaaaa gctggtggtc
     3481 tcttatcaaa gaaaatacga gaataacgta attctgattt attatataat ttcataggct
     3541 tacaattggt ctaaaaatat ctactaccat tttttcagga gaagaattaa cataaactgt
     3601 atagacaatc ccatccgttt gaatatcatt ttcatagaca tatagatcca atttagaata
     3661 cgcatactgt agatactctg gactgtcttc aaaacgaaca tataaacaat atggaacaga
     3721 gatagaatcc tgtacatcat aaatgttact gtactgttga gcattatgag cttgaatata
     3781 aaactcaaaa tcagtcgtta ttaatccatc atcatgaata gtagtaccac aactttttac
     3841 aattaatgga ccaaaaattt gtgcttttaa caactgcaaa tgttgatgaa atttattaat
     3901 ttcctgatct acatcttcta cttttgtatc atgtaacttt ttacagataa ctgactttag
     3961 taccagtttt ttattatctt ttacctctaa cttagccata agtaacctcc tctgtatcta
     4021 acacagcctg tgactgaatt tgttgattca cttgaacgct ctgcaaacca accattctag
     4081 catactttcc atttttcgcc attagttctt catgagtacc atattccata atagtcccat
     4141 tttctaaaaa gcatatctta tcacatcgaa gaattgtaga cagcctgtgg gctactacaa
     4201 ttgttgtctt atccattatt ttattaaaga ttaaatcctg aataatctgt tcactaaatg
     4261 aatctaagtt agaggttgcc tcatcaaata tatacaaatc agctttactc agtagtgctc
     4321 ttgcaatagc caatcgttgt ttctctccac ccgagaaatt ttgaccgcct tcttctacaa
     4381 aggagccata acgattttga agacgttgaa tcgtatcatg aataccaaca atacgacaaa
     4441 ctctcaccat atcctcatat gtaacagaag gattaccaat ttttagatta tcaataattg
     4501 tccctgtaaa gagttctatg gtctgaggaa tgtaggaaat tttcttacgt agtttaccat
     4561 agtcaagatc tgataagtca ttttctccta tacgaatttt cccctcactc ggatttataa
     4621 atcttaataa caaacgaact agcgtacttt ttcctgctcc gctttctcca acaaaagcta
     4681 ttttttctcc ttgtttaatt cgcaaattaa agtccttgat aacaggaggg cgtgaaccat
     4741 aggcaaatgt tacatcttta aattcaatgt catcacataa ggtgaagtct ctaatactat
     4801 aatccaaggc aatgtcttca cgatctactt ccatcaattc ttgaagccga ctaaccgcaa
     4861 cctgtacctc ttgaaaggtt aactgcaagc caactaaatt ctgaataggt tctgtaaaat
     4921 attgacttaa tgtctgaaaa accaagaggt ccccaatagt catttttcca tcaatgataa
     4981 ataaggcgcc cactcccata aataataacc cacccatggt tgaggtcatt gaagatattg
     5041 tagattgaat attctgcaaa acaccctctt tataaccaat ttctaaagta tgaacaaatt
     5101 tttcttcaat tttgtttaaa cgttgttcct catcatgctg agacttaatg gtatcaatgt
     5161 tacgaataga ttcaattaac tgtgaattaa gaagtccatt tgcttccatt tgttcatgat
     5221 taattttttt ataaggcttt ttaaaacagt atataagaat gatgttaaca ataaccataa
     5281 aaactaatat cagaaataaa gattgattaa tagtccaaag gacaaccgca ctaataactg
     5341 acaaggtgat atccatgact aatgaaatag agactgacgt aaacacattc tttatcgtca
     5401 tcgcatcttg aaatcttgtt aaaacatcac caactctacg acttccaaaa aaactataag
     5461 gaagatgaat aatatggtca taatatccca ttaaaaccgg tatatcaatt tttcgagata
     5521 gaaacaaaag aacgtgctga cgaaatgcag atagaagagt ctgtaagaac gacactattc
     5581 caaatactat caaaaacata tacagactat tttttaaagc atatggaatc acctcgtcca
     5641 tgataacttt tgaaaagaga ctcgataaaa taccaatgat acttaaaaca aaggaagcta
     5701 atatgactgt actaatcaat cctttttgat taaaaattaa ccttccaaat aaatcaagaa
     5761 gtccttttcc ctttagtttc cctctttcaa aatccgaatt tggaaccata aaaagagtaa
     5821 taccttgata aatttctccc agttcgtcac ttgacatctt gcgtatgcca ctagcagggt
     5881 ctgctacgta gaacttacta ttccttttga tactatgtag taccacaaaa tgattttgac
     5941 ctaaatcatt cttaacttga agaatcgcag gaaatgttaa ttttggagtc aaatcttcca
     6001 gtgctactcg aacagctttt actgtaaaat tcaacttatt cagacctgaa acaataccac
     6061 tgacagtcgt tccatacatg tctgtaccaa taatttccct aatcttcatg attgatagtt
     6121 cctttttgta agataaaaga actgttgaaa caactgccgc agcacaatct gactggtcat
     6181 gttgcatcgt acaaggatat ttttttaaca tagaaatact caattcttta gaattaatct
     6241 aacttttctt tgatgtcacc caaacctcaa tcactgccca gtagttcttg aattaattta
     6301 ttttcaaccg tatcattctt tgttcttgat atctttccaa gcattgtagt acagcagatt
     6361 tttgcccaag gactgagcct cctttaacaa ctcaaactcc cttacattat atcggccagc
     6421 cccatggaaa gattatagcc agactaatca atgatataat acatactaga atacaaaata
     6481 tttttttatt catacttttt ctcctatctt tattttctac ctacatagta cctcttatta
     6541 agctcatttt aaaattacgt taactttttt cacaaattta tttcgacttt cttttttact
     6601 ttttacattg ctatgataca atatttttaa caaaatttta ttgtttgtta acttttttca
     6661 cgaaaaggag aaaatcatgc gttatgattt cggaaaggtc tataaagaaa tacgtgagtc
     6721 aaaggggctg acccaagaag aggtctgtgg gggtgttctc tcaagaacca gcttatcaaa
     6781 aatcgaaagt ggcaagacaa ccccaaaata tgaaaatatg gaatttcttc tccgtcaaat
     6841 caatatgagt tttgaagagt ttgaatatat ctgtcaactt tatcaaccaa gtcaacgcac
     6901 agagattatg caaacctatc tcaatatgcg ttccattatc gggaccagcg atcttgtcaa
     6961 tttatttcaa aaatgccaag actacctcaa gacccaccac gatctcccta tagaagaaat
     7021 cagagatatg cttgaagttg ttatttatct ccgtcaacac ggggcagggg aactatcaaa
     7081 acacgcagaa caggttgtca aaaaactttg gaaaaaaatt gaaaaacaag atacatggta
     7141 tgagagtgat ctaaaaatcc tcaataccat tcttttcagt tttcccattg aatacctcca
     7201 tctcatcact ggaaaaatat tgcaacgcct agaagtctat aaaaactatc aacatttata
     7261 tgacttgcga atgacaattc tattaaacct ttccacactc tatctataca atcaagataa
     7321 aaacatgtgt aagcaaatct gctacacttt actggaggac gccaagaata agaaaagcta
     7381 cgataggctg gctatctgct atgtccgtat tgggatttgt agggatgatg cgaaacttat
     7441 ccaaaaaggg ttctcccttc tggagctaac cgaggaaact tctatactgt ctcatctcaa
     7501 aaaagaagta gagatctatt accaagcgaa ggaaagataa aaaagccgag ggatttcctc
     7561 gactctttac atattatttg ttcgttagtt cttaatacca gccattgtta aaccagaagt
     7621 ttttagctct gccaagccac atagtgtgtt tactactatg tgtccatgtc accatgaatc
     7681 gccgaacgat ttaacgagaa agtatgactt ttacgtttat cccaactcaa ttatgacatt
     7741 tttttcaaaa gtcaatatat ctcacttttt caacgacaag aaagaggctg ccaatcgacc
     7801 aacctcttat tctgaaccca tcactccatc actttttagc ttcattcgct ttcttagcga
     7861 ctgcaatctg gtattcgact tggtcaaacc ctgtgcctcc caaggaattt cgtctttgaa
     7921 cagcagtttt aggttgcaag tagacataga tatcctcggt aatgagagaa tgataggttt
     7981 gtaattcctc caaagtccaa tcttgaagat ttttagcaga cttgatagag tctagcacta
     8041 atcttcccac aacctcatga gcttctctaa atggcaagcc tttccctgcc aaataatctg
     8101 ccaactcagt tgcatttgaa aagtccttct ctgttgattc ttgcattttt tccttgttta
     8161 cctgcaaact agataacata cctgccaaca catcgaggga atttaaaatc gtttcgaccg
     8221 tatcaaacat tccttcctta tcttcttgca aatccttatt ataagctaaa ggcaaggact
     8281 tcatgactgt caacagtcca aacaaatgcc cataaactct gccagtcttt cctcgaatca
     8341 actcagccat atctggattt ttcttttggg gcataataga ggaacctgtt gtaaacgtat
     8401 ctgacaaggt aatgaattgg tactcaaaac tacaccaatt gatcatttct tcgcaaaaac
     8461 gactcatatg catcatgagt atgctggcat ttgatagaaa ttctaagata aaatcgcggt
     8521 cactaacagc atccaaagaa tttgtataag gttgcttaaa ctccagcaaa tcgctagaca
     8581 attggcgatc aatggggaaa gtcgttcctg ccaaagctgc cgcacctaaa gggcataggt
     8641 ctgtatgctt ctggttaaat tcaaaacgtt cactgtctct ttgaaacata ttgtagtaag
     8701 ccatcaggtg gtaggcaaaa ctaataggct gggcatgttg cagatgggta tagcccggca
     8761 tgatcgtcgc cacatgattt tccgccaagt ctaataaaac acccttaaga tgagccagtt
     8821 tatctaggac atagcctagt tgttccttga gatacaagtg catatctgtc gcaacttggt
     8881 catttcgaga acgagccgta tgtaacttcc ctgcaagggg accaattttt tctgtcagca
     8941 acacttccat attcatatga atatcttcgt ttgcgatatc aaagtcaagt tgacctgcct
     9001 ctagctcttc taaaagctct ttcaagccta cttgaatctt ttctgactcc tccaaactca
     9061 aaatgcccgt ctgacccaac atctgaacgt gggctaaaga accaatcaca tcaaatttag
     9121 ctaatttctg gtcaaaagat atactcgcac caaagcgctc tacccagtct tcgacagtac
     9181 cttcaaagcg accaccccat aattttgtat tcttagccat atcacatcca cctctctatc
     9241 caatcgtcct atttggcgct tttttgaacc tcagaatgaa ccttggttgg aagtccccat
     9301 aactcaataa agccaacagc cgcatcttgg tcaaaggtat ctgcactagt atacgtcgcc
     9361 aaattttcat cgtaaagaga atttggtgat ttccgagcca cgacctgagc gctccccctt
     9421 atacagtttg acttttgcag tcccattgac aactttttga gtctctttaa tataagcaat
     9481 caaggcttgt gtagccgaac taaaccacaa agcattataa atgagatttg ataattcatt
     9541 ttctatgatt ggtttaaaat gagccacttc tctcacaagc gtcaagtcct caatttcctt
     9601 atgagctgtc agcagggtca ctgcacctgg acactcatag atttctcttg atttaatccc
     9661 aaccaagcga ttttccacgt ggtcaatccg tccaacccca tgttttccag ctatttcatt
     9721 gagtttttga atcaaatctg ccaattttaa cacttctcca ttgagggaaa ctggaacacc
     9781 ttcactaaac tcaatctcaa tgtattctgg catatctgga gcttgctctg gagaagttgt
     9841 gatgccaaag gcttcttctg gagcctgatt ccatggattt tccaaaatac cacactcatt
     9901 ggcacgtccc caaagatttt ggtcgacaga gtagggattg tcaaggtcag caggaacagg
     9961 caccccattt ttcttggcat aataaatttc ttcctcccga gaccatttcc attcacgaac
    10021 tggagcaatc acttttaaat tgggatccaa ggctgcaatc gatacttcaa aacgtacttg
    10081 gtcattcccc ttaccggtac aaccatgagc aattgtagtc gctcctatct gatgcgctat
    10141 ttcaaccaat tttttagaaa tcagagggcg gctcaaggca gataccaagg gatacttttg
    10201 ttcataatag gcatgtgact gatgagccac tagcacataa tctgtagcaa attcgtcctt
    10261 aacatcaatg acataagatt ctactgcccc aaccttaaga gccttatcat ggatgaaatc
    10321 taagtctttc ccttcaccca catccataca aactgaaaca acatcatagt ctttctttaa
    10381 ccaggtaata gcaactgatg tgtccaaacc gccagaatag gccaaaatta ctttttcttt
    10441 actcatgttc ttttccttct ttctattttt caatggatgc tttaaaggca tcctcaatga
    10501 gtttgtctaa ttccccagac tccttcaact tttgaatagt tttatcgact gcctctttca
    10561 attccttact atcttttttc atggctaccg agtaggaatc atcttgctct ttttcaaaat
    10621 tgaggtctgc gattgctaaa tcaggattat tttccacaaa gcccttggca actggttctt
    10681 caaagataac ggcatccact tgtcctgatt ttaaatctgt gattaaattc ccatttttag
    10741 gcagagatac gagggaagaa ttttgtagca aatctttcgc catcgtctct tgaatcgaac
    10801 ctttctgggc tccgaccttc ttctgcgcta agtcgttgac agactgataa gtgactaaat
    10861 cagatttttt gacaatgagt ttattttttg cagtatagta gggaatggaa aagtcaaaca
    10921 ccttgctccg ttcatctgtc ttagaaacac ctgatatggc aagatcagct tttcctgatt
    10981 gaacactagc cagtacattg tcaaaactca tgggagatag ttccaattcg acacctagtt
    11041 ctgttgcgat agccttggct aattcgatat ctgaacccac aatctgattt ttcccatcaa
    11101 ccactttttg gtattcaaat ggagcaaaat ctggattgag ggctacaacc aattttcctt
    11161 tggacttgat ggcttcaatt ccagccgatt gattgttact acaagcaaat agtaggggga
    11221 gcatcactaa accaaacatc gtcatcaaca ccttcttcat cttattcata acaaagcctc
    11281 ctttatgttt attccttttg tttgtataaa taatacacct atcttcatcc tttgtcaagc
    11341 ctaaattgta atttttttca atttaaaaca gaaaaaccta ggaaaattac ataaaaatgt
    11401 cattcctagg cgtatttatg ctattcctct ctggaaaata tgagtattca gtcagtcaaa
    11461 agaagctgaa cgaactcatt ttccctcgcc caattcaata atccgatgac attgttgggc
    11521 tacataagca tcgtgggtca caataatgac tgttttccct tctcgattca tctctaagag
    11581 aaacttcaag accaaatctc tattttcagg atccagcgaa cctgtaggtt catcggctaa
    11641 aatcagctgg ctgggtttta agatggctct agcaactgca attcgttgtt gttcgccacc
    11701 agacaactcg gagacctttt gatgcaaagt agctgacaaa cctactctct ctaaaatctc
    11761 ttccaccttt ttgagcttat ctttcttaga caattttaca tatttcagcg ccagcataag
    11821 attgtactcg accgtttcat catcaatcag ggcaaaattt tgaaacagat aagagatatg
    11881 ttcacggatt attgtttgcg acttagcaga attgaccgct agatttgtct gaccaaaaat
    11941 ctcataccgt ccgctataat caccatctat caaacccaat aaatttaaca aggtcgactt
    12001 cccactacca ctcttaccaa caatggcaac caaatccccc tgatcaatcc tgagagacaa
    12061 gcgatccaaa atcactttcc ccccaatggt tttggtaata ttttccaact caatcataag
    12121 atgccccctt tcaataactc tactagactt cttttctcca tcctagaagc caaggctagc
    12181 acaaatagta tatccaaaca tgtaaagcct gcaaacagca ggagtggcaa gaacgcatgg
    12241 gcaaagaaaa tcaagactag aagagggaga ctatagccca gcaagagcag aacgaggaga
    12301 ggacggtagc gatcgaccag tttccacccc ataaacttct tggtaataat atccctgcgc
    12361 ttcaataaga aagttattac tagtaagaag taggaaatca tcatgctaaa gagaccaaac
    12421 aaagcaaaga gtaggttaaa attccgaaca gcatctcgat aagaatccac tttctcttgt
    12481 tgaatggctt gaatagatga aaattttaaa taatttccat ctgacaattt ctcaactaac
    12541 tctgtaatct ctttttgatg ttgaaccgta ttttcaattt taatcggatt atttaagcca
    12601 gttgttgaca gggaggcttt ctcatcccac atcatatcag aatcattgac caagctaata
    12661 attggattgg agagattttc ctttcgctta tcactatatg ggaaaaatga ccaatctcct
    12721 tcataatagg caatctcgac atccatctcc tctatcgttc gtttttgctg ctcttcatac
    12781 ttcatcgaat gaaaggcaat taacttcccc aagagctgat ttttatcttc ttcacctttc
    12841 gtacttgctg gcatcaaaat aactttttta ataccagtat ctggtagctt gaatcccttg
    12901 ctctttagaa aattgcgatt ggcatagtaa acatccaccg tatctgttaa ctgatattgc
    12961 tgaatctgtt ctgattggac aaaatttttt acaggaagac tgctactctg cacatagccc
    13021 gcctgcgttt tttctactaa atcctgataa aatcgataga aataatccaa tgccttccct
    13081 gagcctgcca actgctcttg ccacaagttg tcattgagtt ggaaagtttc taaggtcaga
    13141 taatttcctt ggctgatcca ctgtttctgg taatccaact ctttgttttc ttgctccaaa
    13201 ctttttccta ccccaactag caagactgtc agtaaaatgg tagttccaat tttcatcaaa
    13261 taattaaaga gtagaccaaa tttgaaagat gaaaaacctt tcagcagaga gctgattgtc
    13321 attttttgga ttaagaggta agtcaaccaa ctgataaaga gataaagctg caacagcaaa
    13381 aaatgagaca accacagcat aggaaacaaa tcttttggct tataatcaag caagaaaaac
    13441 acgcctagat tgatcacaag agccccacct aggaggaggt aaaggttgcc ttttacaaca
    13501 tcagctaaaa cagccctatc ttgaaaacca agtaattttt gtaccccaac tcgtttcatc
    13561 tccatcatcg gttgatacac tgtcactaac acaagaagca aaatagccaa gacaaaaaca
    13621 atggcagata aaagcaaatc tcgatttatg acttccactg cacttttgta ggtcggctct
    13681 agcaaggtag cctggtctat cttgaaaaaa tcgctccatt tctgtacaat cctatccttg
    13741 tccatctctt gtgtagaagt tatcgtatag cgaccattta aactacgaga tgtatccttg
    13801 atataggttt gaaaagtcat aagctgaata ggtttggctt ttagaaaggt cggaatcgta
    13861 ccaagtttat tggaaatttc tttattacta tagactcctt caccatctgt ggtaaaatca
    13921 agagaagaaa tcccaaactc ttggtagggg aaggtatctt tatcaaaaac accagacttg
    13981 accacctcat cgccattatc tactttgata atagagacct tgtcttggct tgcaacctct
    14041 tcaaaaaact ggagaatcgt ttctgtcggt ttagtgacat ctttcaagta caagtcaaat
    14101 gaataagttg tcttgcccat ctcctgaatc cgcactattt ctcgtgtaac atttacattc
    14161 aggacaaaaa acgtagtaca cagcaagagc aggagcatac aaagatgact gatttttttc
    14221 atagagatac ctcccgtgaa gaaactccct ctccaaaagg ggagggagaa ttttaattaa
    14281 aatccataga aatattccat tcccgttggt gggaatttga agattgaagc ccgagcccac
    14341 gcctcagctt ctgcacgatc cttagagaat ttatttccat ctttaacagt tgctgtatga
    14401 gagcgagtag aatgtaaata atcagaataa ccaaaatttc ctgaccatcc tactccatag
    14461 ttccattgac caccatcaac ccatactgcc aaaacacttg tagaagcaag tatactacat
    14521 accattaaag atgcaacact aattttctca tgatttattt ctcctattat cgagagctat
    14581 tccgtagaaa cagctatagt aatttatcaa caagtatcgt agtccattgg actcaccttc
    14641 cttctatctc atttcatttt taaacaacga acttactctt aactaggaga atagttctct
    14701 tctgtatcag gtctttgaac aaccagagta gtattaatca cactctaact attactcaag
    14761 aatcagccca ataaacaata tggatagcgc tttcaaattt ttaaactcta tcccatcctt
    14821 ttatctatat tatgagcgaa aatataataa ctgtcaagta atcgaagtga attttataaa
    14881 aaaaattaca agccaaattt gcaaagttta caataagccg ctagggcaat cgtctatcag
    14941 aatgtccgtt tatttgtcaa taatccgaga aaatcttgca acgcttagaa gtctataaaa
    15001 actatcaaca tttatatgac ttgcgaatag caatcctgct caacctttcc acactctatc
    15061 tatacaatca agataaaaac atgtgtaagc aaatctgcta tactttactg gaggacgcca
    15121 agaataagaa aagctacgat aggctggcta tctgctatgt ccgtattggg atttgtacag
    15181 acgattctaa acttatccaa aaagggttct cccttctgga gctaaccgag gaaacttcta
    15241 tgctgtctca tctcaaaaaa gaagtagaga tctattacca agcgaaggaa agataaaaaa
    15301 gccgagggat ttcctcgact ctttacatat tatttgttcg ttagttctta ataccagccg
    15361 ttgttaagcc agaagttttt agcagctgtc catgaaccgt aacgtcctgc aacgtaggca
    15421 tctgctacac gttcttggtt ttcagctgag tagtcaccgt tcaggtatga atctgttaat
    15481 tggtaacgtc cgatataacg tccatttgta gctgtatagc taccacctga ttctttttga
    15541 gcaatccatt ctttggcttc tgcttcagat ccgcttacag ttgaaacaac tgtttcactt
    15601 actactggag tttctgctac tggagctgaa acagtttcat cttgagcggc tggtgccgca
    15661 taagtagctg gcgctggtgt tgcaacaggc gctacagggc catcgataac caactcttga
    15721 ccaacataaa tcaaatgaat gttatcaatg tggttgtttt ctgccaattt ttcaactgtt
    15781 gtgttgtgag tttcagcgat ttctgaaagt gtatcacctt ctttaacagt gtaagttgat
    15841 gattcttgag cagatacaaa tgatggagca aatactgcaa acaaggcagc tactcctgca
    15901 agagttgctt taatcttttt agttattgat ttcatattcg aaaattctcc ttctttctat
    15961 agttctatca taccctttaa atattaccgt ttcttgacac ttttatgtag aaatattaca
    16021 aaattatttt ttatttccct aaataaggtg ttttttgtca taaaagatac tatttttatg
    16081 ctatttgagg tattagaaag gttttcatcc acaattaaag ccaagcccct cattctttga
    16141 tataatagag ataagaattt ttataagggg aaactgatga aatcacttcg ttttcaatct
    16201 gtctttgata tcatcggacc agttatgatt ggcccatcta gtagccatac tgctggtgct
    16261 gttcgtattg ggaagattgt ctcttccatt tttgatgata ctccgacaga agttgaattc
    16321 caacttttta actcatttgc caagacctat cgtggtcacg gaacagacct agcccttgtt
    16381 gcaggtattt tgggaatgga tacagatgat cctgaaattc caaatagtct ggaaattgcc
    16441 cacaagcgtg gtatcaagat tgtctggacc attcagaaag acagcaatgc ccctcaccct
    16501 aacaccacta aaattactgt taaaaatgcc cacaagacca tcagcgtgac tggtatttct
    16561 atcggtggag gaaatattca ggtaaccgaa ctcaatggtt ttgccgtctc tctcaatatg
    16621 aatacaccga ctatcatcat cgttcatcaa gatattccag gtatgattgc ccttgttaca
    16681 gaggcgcttt cccgctatgg tatcaatatc gcccagatga atgtcactcg tgaaaaagct
    16741 ggtgaaaaag ccattatgat tatcgaagtt gacagtcgca actgtgatga ggctatcgaa
    16801 gaaattcgaa aaatccctca tctccacaat gtcaatttct ttaaatagga ggaagcatgt
    16861 tttattctat caaagaattg gtcgagcaag cagatctgga ctttcaagga aatgtcgcag
    16921 aactcatgat tacaacagag tttgaattga ccggtcgcga acgtgaagaa gtcttccttc
    16981 tcatggaacg caatctggaa gttatgaaag cctctgtcca acttggcctc aatgaaaata
    17041 aatctcgtag tggcctgaca ggtggagatg ctgccaaatt ggatcactac atcgaaaacg
    17101 gaaaaacttt atcagattac acgattctct ctgctgcccg aaatgctatc gcagtcaatg
    17161 aacacaatgc taaaatgggc ttggtctgcg ctactcctac cgcaggaagt gccggttgtc
    17221 tcccttccgt tctcactgct gctattgaaa aattagacct cagccacgag caacaattgg
    17281 atttcctctt tgctgctggt gcctttggac tagtcatcgc aaacaatgcc tccatctcag
    17341 gtgctgaggg tgggtgtcaa gctgaagttg gttcagcctc tgctatgagt gctgccgcct
    17401 tgactctggc tgcaggtgga acaccttatc aggccagtca agctattgcc tttgtcatta
    17461 aaaatatgct aggcctcatc tgtgaccctg ttgcaggttt ggtcgaagtt ccctgtgtca
    17521 aacgtaatgc catgggagct agctttgctt tcatcgcagc agacatggcc ttggcaggta
    17581 tagaatctaa aatccctgtg gatgaagtga tcgatgccat gtaccaagta ggagcaagca
    17641 tgccaactgc ctttcgtgaa acagctgaag gtggactcgc taccacccct actggtcgtc
    17701 gcctccaaaa agaaattttc ggagaataag cttatcaaat aggagaaacc atgacctcta
    17761 tcacagcgat ttttttcgat ttggatggaa ccctcgttga tagttctatc gggattcaca
    17821 atgcctttac ctataccttt aaagagctgg gggtgcctag ccctgatgcc aaaactattc
    17881 gtggttttat gggaccacct ctcgaaagta gttttgcgac ctgcctgtcc aaagaccaaa
    17941 tttctgaagc catgcagata tatcgttctt actataaggc aaaaggcatc tatgaagctc
    18001 aactctttcc tcagattata gacttgcttg aggagttatc gagcagttat ccactctaca
    18061 tcaccacgac aaagaataca tctactgctc aagacatggc caaaaacttg gaaatccatc
    18121 atttctttga tggcatttat ggttctagcc ctgaagcacc ccataaggca gatgtcattc
    18181 accaagcctt acagacacat caactagcac cagaacaagc catcatcatc ggagatacca
    18241 agtttgatat gctgggagct cgagaaacag gtattcagaa attggccgtc acttggggat
    18301 ttggagagca agcagatcta ctaaactatc aacctgatta tatcgctcac aaacccttag
    18361 aagttttggc gtattttcaa tagcatagac tgggcgacaa ctctatttca gaagaaaatg
    18421 aagtaatact caatgaaaat caaagagcaa actaggaaac tagctgcggg ctgctcaaaa
    18481 cactgtttta aggttgtaga tggaactgac gaagtcagct caaaacactg ttttgaggtt
    18541 gtggatagaa ctgacgaagt cagtaacata tacatacggc aaggcgacgt tgacgtggtt
    18601 tgaagagatt ttcgaagagt ataagtctct acataataaa acgcatatta ccaaggttgt
    18661 gtaacttgat actatgcgtt tttctttttt attcaatgtg ggttgtttga ttagcaaagg
    18721 cgataatagc ttgcttcaac tcatctccac tgagagtgcg gaactctcca atcttttgat
    18781 tgatggattc taaaggcatg acattaacct taccaacgaa aatcttatcc ataacttgat
    18841 tatcaacgag ttcggttgat accaagagtt cttcgtagag tttttcacct gggcggattc
    18901 caacttcaac gattggaatt tcactttcag tgtggccact tagaagcacc atcttcttgg
    18961 ccaagtcata aatcttgact ggtttgccca tatcaaggat aaagacttcc ccatctttgg
    19021 cataagcacc agcatggata accagacggc tagcttctgg aatggtcata aagtaacggg
    19081 tcatgcggaa gtctgtcacc gttacaggcc caccttcagc aatctgacgt tcaaagactg
    19141 gaatgacact accacggcta ccaagaacat tcccaaaacg aactgcacag taggttgatt
    19201 ggctacgttg gttaaagcca gtgacaatca actccgccac gcgcttggtt gctcccataa
    19261 catttggtgg attgactgcc ttatctgtcg aaatcataac catcttagac actttagctt
    19321 catcaacagc cttagcaaca ttgtaagttc cacggatatt gtttttgaag gcttcttttg
    19381 gattgcgctc catcatagga acatgcttgt gggctgccgc atgataaaca atagcaggtt
    19441 tgtactgctc aaagacttgc aacaaacgat catagtcttg aatgtccgca atcacaggta
    19501 cataatcaat cccttggaac ttacgaatca attcatgata aacaaggtag attgagtttt
    19561 ccccatgacc gagtaagaca atgcgttcag gattgaagcg actaacttga cgacagattt
    19621 cagaaccgat tgaacctcca gctcctgtga ctaagatggt cttacctgtc agttctgcac
    19681 ccagacgcga ttcgtcaaga cggatttcct gacgacccaa aaggtccgta atatcaattt
    19741 tttggaagcc agtacctgct tggtgaaggc cctgaacaac agtttcaacc ttaggcatct
    19801 tgtaacattt gacacccagc ttattacaca tctgcaagat acgctcatat tctgacggat
    19861 ccagcgacgg aatcgcaacg atgacacgct cgatttgatg gcgtttggct aattcaggca
    19921 gattgtcata agagcccaaa acaggaatac caccaagttt ttgacccttt ttcttagaat
    19981 ccttatccaa aataccgacc agttctaatt cactggttgg atgttggtaa ctatccataa
    20041 agagagcccc accatcaccg gcaccaatca agaaggtccg acggtgttct ccatcaccac
    20101 tacctttttt gcgtctggag tagattaact gccaagtaat ccgtggcaat aaaatcaaga
    20161 aggtactcaa caagataaag agaatgatga aacggatgga gaagagtggc aagaaggcat
    20221 aacagatact atatgacaag acactgctag cagtcacacc aaaaaagatt ttcatgaaat
    20281 ccgtaatctt gctgtaacga ctaatgctcg cgttcaaccc ccaaaaacca atcatcaatt
    20341 gatagaacaa gaaggccaaa ctcgtataga taatgtagtc aacaggtgct ggattaatca
    20401 gcccataaaa taaaatataa gatacaatta tggaaaccac catactgaaa atatcaaata
    20461 ttccccagaa aacctgcttt tgttgtttat ttaaaatttc caccagatca atcacataat
    20521 ctgttagttt tttattcata gaaatctact cctccttaaa agagttttac tatattgtta
    20581 ttatagcata tttttattta tttggcttat tttatccaaa taaattattg ttctcttgta
    20641 ctccttcttc catttaaatc ctctaattaa acctacaatc gtcccaaggc cataagcaaa
    20701 gtgaatggaa aataaaagaa agggcatcac aattagaaat ccatttttat gttttaataa
    20761 agtcagcaaa gtgagtaatg acaaaagtag aaaataggca cctaatagta aagttatgaa
    20821 tacgaatgtg atcggtaaca atgctagact aaacacaaga ctcaaaacaa ataaacaagg
    20881 aacatagtga aataatgata aacacttaaa ctgaacatga cttgtcaagc caatccacaa
    20941 accatttgaa tacttttgat gcagcatttt cttgaatgtt ggtcgaatat actgataaga
    21001 tagaatactt gggctatagc ggattttata accatgttct cgaattctat aatgaatatc
    21061 attatcttca gttcggccaa gttgctcatt tactaaacca accttctgga aaacctctcg
    21121 tttatacatc ccatgaaaaa tagaagaaac atatctatcc tcggaactat ttcgataatt
    21181 ggcaatgcta ctgccaaaca tattttcctc aacaagatgc aaggtctctg cccattttcc
    21241 ttttccttcg acaatcgtcg gtctaggccc cccacagaca aattcacctt gttgaataat
    21301 agccacattg ttcattacaa aactctcagt aacttttgaa tgagcatcaa tttttaaaat
    21361 aaggtcccct acagaatgtt taactcccag gttaaaacca ctagcttgat ttttcttagg
    21421 attgttatac aatctaattg agttaaactc tgtatcttcc tttataaatt gctgaatgat
    21481 agctgtggtc ccatctgtgg acatagcatt tataaataga atttcaacat tctctttagg
    21541 ataggtttga ttttttaagt cttcaattag accaggcaga tatttttctt cattgtatgc
    21601 actgattaca atggatacga gaagactttc aacattttga gtcactacta ctctcctgaa
    21661 tttttccttc caccattcta tcataatttt caagactttc ccatttttat cttgaaagcc
    21721 agaaacctta cttgacatta tagtgaaaaa gccttaaaat aagctgttat taaaatcagc
    21781 tagaagaggt attatatgaa aaagcaatca ctcttttttg ttccaggtat tatcctgatt
    21841 ggtgtttcct tgcgaactcc ttttactgtt ttacccatta ttttgggaaa tatttcgcaa
    21901 ggtctggagg tagaagttag ttcgcttggt gtcttgacca gcctgcctct ccttatgttt
    21961 accctctttt caccattttc tacccgactg gctcagaaaa tcggcttgga gcatctcttc
    22021 acctacagcc tcttcttctt gaccatcggc tcactcattc gactaatcaa tctgcccctg
    22081 ctctatctag gaaccttgat ggttggggca agtgtcgcag tcatcaatgt cctgcttcct
    22141 agtcttatcc aagccaatca accaaagaaa attggttttc tgaccacctt atatgtaacg
    22201 tctatgggga ttgcaacggc tctggcttcc tatctagctg tgcccattac acaagccagt
    22261 tcttggaaag gacttatcct cctcctcacg ttactctgtc tagcaacttt tttggtctgg
    22321 ctcccaaatc accgctataa tcatagacta gctccacaaa ccaaacaaaa aagtcaaata
    22381 aaggtcatgc gtaataaaca ggtttgggca attattatct tttcaggttt tcaatccttg
    22441 atcttttaca ccgtcatgac ctggttacct accatgtcta tccatgcagg tctatccagt
    22501 cacgaagctg gcttgctgac ttctatctta tctctgatta gcattccttt ttcaatgacc
    22561 atcccaagcc tgacaaccag tttatctact cgcaaccgtc agctcatgct cactctggtt
    22621 tcactagctg gtgtggtcgg catttccatg ctctttttcc caatcaataa tttcatttac
    22681 tggcttacca tccatctcct catcggaacc gcaaccagcg ccctcttccc ttatctcatg
    22741 gtcaactttt cactcaagac aagcgcccct gaaaagacag cccaattgtc cggcctatct
    22801 caaacaggag gctatatcct agcagccttt gggccaaccc tctttggtta cagttttgac
    22861 ctgttccact cttgggtacc agctgtagct gccctcttgc tcatctatat cctgatgact
    22921 gtggccctct ttacagtgga cagagcggat aagatccttt aaacttctag ttttagctag
    22981 aagtttttta tatataaaaa ttttacacat ttctcttgac agggctttct tttagatgta
    23041 caatgtatgt gtagaaaaat tatatataaa aatcttacac attagaaaag gaggtttccc
    23101 atgtactttc caacatcctc tgccttgatt gaatttctca tcttggctgt actggagcag
    23161 ggtgattctt atggttatga gattagccaa accattaagc tgatcgctaa tatcaaagaa
    23221 tccacactct atcccattct caaaaaattg gaaggcaata gctttctgac aacctattct
    23281 agagagttcc aaggtcgcat gcgcaaatac tactccttga caaacggtgg tatagagcag
    23341 ctcttgaccc taaaagatga atggacactc tatacagaca ccatcaatgg catcatagaa
    23401 gggagtatcc gccatgacaa gaactgaata cctgactcag ctagaactct atctcaagaa
    23461 actacctgaa gctgaccgta tcgaagccat ggactatttc agagagctct ttgacgatgc
    23521 tggagtcgaa ggagaagaag aactcatcgc tagtttggga actcccaaag aagcggccca
    23581 cgaagttcta tccaatcttc tcgataaaaa aatcaatgaa gcacccgctc aaaaaaataa
    23641 ccgacaaatt ttacatatcg ccttgttagc cctccttgca gcacctatcg gcattcctct
    23701 gggaatcgcc atcctcgtga ccctgttcgc aatccttgta gccgctttga ctgtcattct
    23761 ggctttcttt gcagtttcca tactgggtat catcggcgga ttcctatttt tagttgaaag
    23821 tttcactgtc ctcgcccaag ccaaatcagc ctttatcttg atttttggtt ctggtttact
    23881 ggctatcggt gcttcttcgc tagttttact tggcatttcc tatgtaactc gcttcttcgg
    23941 tctactcatt gttcgtctgg tacaatttgt tcttaaaaaa ggaaagagag gtaaccagca
    24001 tgcgtaaatg gacaaaagga tttctcatct ttggtgtggt gactaccgtt atcggcttta
    24061 tcctgctttt tgtaggtatc caatctgacg ggattaagag cctactttcc atgtccaaag
    24121 aacctgtcta tgatagccgt acggaaaagc taacctttgg caaggaagtc gaaaacctag
    24181 aaattactct ccaccaacac acgctcacca tcacagactc tttcgatgat caaatccaca
    24241 tttcttacca tccatctctt tctgctcacc atgatcttat caccaatcag aacgatagaa
    24301 ctctgagtct cactgataag aaactgtctg aaactccgtt tctctcttct ggaattggtg
    24361 ggattcttca tatcgcaagt agctactcta gtcgttttga agaagttatt ctccgactac
    24421 caaaagggag aactctaaaa gggatcaaca tctcagccaa tcgcggacaa accaccatca
    24481 taaatgctag ccttgaaaat gcgaccctca atacaaacag ctatatcctc cgaattgaag
    24541 gaagtcgtat caaaaacagt aaactcacaa cacccaatat cgttaatatc tttgatacag
    24601 ttcttacaga tagtcagcta gagtcaacag agaatcactt ccacgctgaa aatatccaag
    24661 tccatggtaa ggttgaactg actgccaaag attatctcag aatcatccta gaccagaaag
    24721 aaagccaacg aattaactgg gacatctcaa gcaactatgg ttctatcttc caattcacaa
    24781 gagaaaagcc tgaatcaaga ggtacggaat taagcaaccc ttacaaaact gaaaaaaccg
    24841 atgtcaagga tcaactcatt gcgagatctg atgataatat tgatctaata tccacaccaa
    24901 gcagacgttg acaaaaacgc ttacatctgc tactatggaa gcatatttat ctaactaaaa
    24961 aggaggttct accatgaaac aagaatggtt tgaaagtaat aattttgtaa aaacaacaag
    25021 caagaacaag cctgaagagc aagctcaaga ggttgcagac aaggctgaag aaacgatagc
    25081 cgatttcgat acaccaattg aaaaaaatac tcagttagag gaggaagtct ctcaagctga
    25141 agtcgaactg gaaagccagc aagaagagaa aattgaaact cctgaaggcg gtgaagcgag
    25201 aacagaaata gaagaaaaga aggcatctaa ttctactgaa gaagagccag acctttctaa
    25261 agaaacagaa aaagtcacta tagctgaaga gagccaagaa gctcttcctc agcaaaaagc
    25321 aaccacgaaa gagccacttc ttatcagtaa atctttagaa agtccttata tccccgacca
    25381 agctccaaaa tctagggata aatggaaaga gcaagtgctt gatttttggt cttggctagt
    25441 ggaagcgatc aaatctccta caagtaagtt ggaaacaagt atcacacaca gttacacagc
    25501 ctttctcttg ctcattctgt tttctgcatc ttcctttttc tttagtatct atcacatcaa
    25561 acatgcttac tatggacata tagcaagcat taacagtcgc ttccctgagc agctagctcc
    25621 tttaactctt ttttctatca tctctatcct agtagcgaca acactcttct tcttttcatt
    25681 cctcttgggt agtttcgttg tgagacgatt tatccaccag gaaaaggact ggacgctaga
    25741 caaggttctc caacaatata gtcaactctt ggcaattcca atcttcctca ctgctattgc
    25801 tagtttcttt gctttctttg atagcctacg atttacagct ctcttgtgtg tgattagcat
    25861 tggaatcatt ctgcttgcca gtctccatat cattacaaga ccaagtcaag caagtgaaac
    25921 cgactccttc tatcaattat tcttgtctgt ccttgtgaac ggagttatta tcctcctctt
    25981 ctttgtagct gaagtggcac tgattggaga ttatcttcgt atcttggcct ttctttaaaa
    26041 ttcaatcctg tcatccttgg caggattttt tctatgtcaa aaaagcaagt cgattgacct
    26101 gcttctgctt tacttttctt gtgctaatgt tttaaaatct tgtactagga tgggttgtat
    26161 ttctatttga ttggcgtcta gttggtggta aaaagatggg ggtaatacct ctaagaattt
    26221 tgcatagtcc aagcgagcta tggattcata gtcttctggt ttagagtcgt ctcctgtgat
    26281 ttgaaccaag tcaatctccc actggacttc cttatccacc aattgctcaa tataggctgc
    26341 aggaacaaac ggttctctcg gtaaaactgt cttgactcct tgagctagat ggtaaacgcc
    26401 atgcactttt ccttctccat cctgatagaa ggaaactctc gcaaagtagg catctgtctc
    26461 ttgaaccgca cgcacacgcg cttcaaactg agccaagcca tctgcctcta aaaagctttt
    26521 caagttctca cgactaaaga aaataggatt aaaaattcct tggcaaatcg taaaacgagc
    26581 tacagggtct gctagatagg cttgaatact tgcttggaaa taagcttcaa aatcaaaacc
    26641 atctagccct tcaatatcag tccctgtata agcaagcaaa gcatctaaaa atgacttgat
    26701 aatctgccag tctgtctttg ttagtaggtc aggaagatcg acacggtaag ccttttgacc
    26761 atcagcataa ctgaccttaa aaagaaattg tgattgcccc actatcgcac actcaatata
    26821 ctcgagacga ttaagaggct gacggagata gactacatca tagctatgtg actccaaacc
    26881 gtctagcaag gccaagatcg acttggcatt caaaatctct tgttctccta aaatgctctg
    26941 tttatttgga ataaaaaatg ttttcgccat aactggactc ctttgaaatc gtttacatac
    27001 agtatacctt atttttctga aagatacaaa aacaaacttt agatttttga gtacaaagca
    27061 tagaaaaaca gcccctgagg actgtttgat cttatacagt agctgcctga tccaagcttt
    27121 caccgatagc ggctaggcgc tcaatcactt cagcttgtgt caattcattt tttgaaacat
    27181 agcggttacg tgggtgaaca cggcactcgt gtgagcatcc acgaaggtac ttgtcttcat
    27241 tttcttctga tgtcaagata cgacggttac agaagggatt tccacagttg acataacgtt
    27301 cacatggtgt tccatcaaac cagtctttcc ctacgatggt tgggttgaca tggttgacat
    27361 caacggcgat acgctcgtca aatacgtaca tttttccatc ccaaagctca ccttgaactt
    27421 ctgggtcttt accgtaagtt gcgattcctc cgtgcaattg gccgacatct ttgtagcctt
    27481 cacggaccat ccagcctgag aatttctcac agcgaacgcc acctgtacag taaaccacga
    27541 cacgcttgtc catgaatttt tccttgttat cacggaccca ttgtggtaac tcacggaagt
    27601 tgcgaatatc tgggcgaata gctccacgga aatgtcctag gtcgtactca taatcgttac
    27661 gtgtgtcaag gacaacggta tctttatcaa gaagcgcttc tttgaactct tttggagaca
    27721 agtaagcacc tgttgtttca agtgggttga tgtcattgtc aaagtcgttg tcttccaaac
    27781 caaggtggac aatttctttc ttgtagcgaa caaacatctt cttgaaggct tgttcatttt
    27841 cttcgtcaat cttgaaccag agttcttcca ttcctggaag gctgtgaacg tagtccatgt
    27901 atttttgagt tgtttcatag tcacctgaaa ctgttccgtt aattccctcg tcagcgacta
    27961 ggatacggcc tttaaggccg attgatttac agaaagccaa gtggtctgca gcaaattgct
    28021 ctgcattttc aattggagta taaaggtagt aaagtaagac acgaatatct tttgccataa
    28081 gatttgttct cttttctatt cttaaatttt ctaaattttt tatttatcta cactagtata
    28141 cctgcttgag aaaacgaatg caatttattt gactggaaat tgtagagggt tctcgaaatt
    28201 tcctgaatgg ttaaaataag gacaagagaa aacatggata tctatatcct tgtgccaaaa
    28261 aaaccactgc cctccccaga ccaacctgag gaaagcagtg attcttattt taggagttag
    28321 gaatgaatac acgaaatcaa tttagctgat tattttttgt ttttcaagaa ttcatcgtat
    28381 tgtttttgca tttcgttcaa tactttttcg taggcacctt cagatttcaa tttttccatc
    28441 aattctggaa tcgctttatc tgggtctaca gtaccagtgt tgatagctgt atcaaattgt
    28501 tgcattgtgt tagcaatagc tgagatttca gatttcacat tgtcagtatt gaagataaat
    28561 ccaagcgctg gagattcttt agcttctgcc aattctttct tagaattttc gatttgttgg
    28621 tctgtaacgt tttcgttgat gtaaaggatc cagttgttac cagtgttcca tccacccatg
    28681 tgagtgtttc ctttgtagcc atcaagaacg cgaacacggt tttctttacc ttcaattttt
    28741 tcccagttct tgccttctgg accgtaaaca agaccgttca agagttctgg attcgtattc
    28801 aagaggttca agatttccat tgatttttct ttgttcttag agttgtttga gatgacaaag
    28861 ttagcaactt gtgttgtttg gtttttcttg atgaagttag taattggttt gatttggata
    28921 tctttgttgg caacacgtga aagcaagctg ttaccgtagt cagctggtcc tactgtttct
    28981 tcacgaacga accaagtatc ttgttgaagg tcaaaggaag tatcgcttgt tgcgacatct
    29041 tttggaatgt agccagcttc atagaatttg tgaagagtct tcaagtgttc tttgaaacga
    29101 ggcacttcgt aacggtttac aactttagta gtatcgcctt caaggtcaat aacgaatgga
    29161 agcccgtttg ctactgggta gtcaaagtta tcagatggga tgaaaacttt accaatagca
    29221 aatggtacta cgtctggagc tttttcttta atttgtttca agactggctc aagagtttcg
    29281 taagaagtaa cacctgaaat atcgatacca tatttggcaa ggagagttcc gttgaaggca
    29341 aagttttgag atgatgcaac gttggctgca actggaacag cgtaaatttt accgtttaca
    29401 gtattaccct tgatgtaagc tgggtcaagt gccttgtaaa ggtctttacc ttcttttttg
    29461 tacaattctg tcaagtcagc ataagcacct ttttgagcat ttacaatata gttatctgca
    29521 aaggcgatat catagttttc accagatgat gtgataactg acattttctt accatagtca
    29581 ccccagccaa ggtattggat atccaattta gcaccaactt tttcttcgat gattttgttg
    29641 gcatttgcta acaattcatc caagttatct ggtttgtcac cgatttggta cattttgata
    29701 acaggtttgt cacctgaatc agcagctttt ttgctgttac ctgtcaagtt gccacaagca
    29761 gcaagacctg cagccagagc gactacacta gcagatgcaa aagcatattt tttccagttt
    29821 ttcatgataa aaactccttt ttttattttt aaacttataa acaatgtaat gatcttatac
    29881 tcaatgaaaa tcaaagagca aactagaaaa ctagccgcag gctgctcaaa gcactgtttt
    29941 gaggttgtag ataagactga cgaagtcagt tacatatatc tacggcaagg cgacgttgac
    30001 gcggtttgaa tttgattttc gaagagtatt aacttcacac aagggaagtt gggaactgag
    30061 aaatgttctt tttcaataag cactattctt tcacaccacc gatagtcaaa ccttttacaa
    30121 agtagcgttg gaagaatgga tataaaatcg cgattggaag ggttgcaacc acaaccatgg
    30181 ccatacgacc tgtttctttc ggtagagcaa ctcccagttg accagtcaag ccgaccgctt
    30241 tggcaatgta gtccatattt tgttggattt gcatgagcaa atattgcaat ggatacaagt
    30301 tatcactctt gatgtaaaga agggcgttga accagtcatt ccagaaacca agagctgtca
    30361 aaagcgtgat ggttgcgata cctggtagtg acaatggcaa acagatttgg aagaaaatcc
    30421 gggcctcact ggcaccatcg atacgagccg attcgagaat ggcttctgga atggtcttct
    30481 tgaagaagga acgcatcaag atgatattga atggtgagag aagcattgga acaatcaagg
    30541 cccaaacagt gtccccaagc tgaagtacac gggtcaccat gatataacct ggtaccaaac
    30601 cagcgttgaa caacatactg agaaggacga agatggtaaa aaatctgcga tacttaaagg
    30661 ttgtccgtga aatagcgtag gcataggttg ttgtgataaa gacatttgtc aatgtcccaa
    30721 ctacggttac aaagacagag atgaagaggg cttgtaggat tttatcctta aactgtgcca
    30781 aaaactcaaa accgtctaag ccaaattggg atgggaagaa gctatagccg tattggagga
    30841 ggcttttctc gtctgtcact gaaataatga taacgaatac aaaaggtagg atacaagaga
    30901 gggcaatcaa acccgaaatg atactgaaga agatatctgc tttcttactg aaggagtgaa
    30961 tgccgacatt atcaattttt tcttttttaa ttttcttttt tgccatattc tcctcctttc
    31021 tagaacaaag ctgagtttgg atcgactcgt cttgcaagca agtttgatag gataaccaga
    31081 atcaaaccaa caacggattg gtaaagaccg gctgctgcag ccataccgat atctgctgtc
    31141 tgagtcaaac cattaaagac atatacgtcc aaaacgttgg ttacattgta aagctgacca
    31201 gcattgtgtg ggatttgata gaagagaccg aagtctgcgc ggaagatatt tccgactgca
    31261 aggatggtca atacagttac aagcggagtc aactgaggaa tggttacgtt gcgaatacgt
    31321 tgccacttgc tagctccgtc cactgtcgct gcttcgtagt aggttggatc aattcccatg
    31381 atcgtcgcat agtacatgac actgctatat ccaaatcctt tccaaatacc taggaaaagt
    31441 aggagatagg gccagatgcc caggtcagcg tagaaattga cttctttgag accaagactt
    31501 tccaatagat gattgaacac ccctttatca atatttagga aggcatctgt aaagaaactg
    31561 atgataaccc aagacaagaa gtaagggaac aacatagaag tttgaaaaat cttcaccatt
    31621 ctcttagaac ggagctcgct gaggataatg gcgatcccta cagatacaac taaacctaga
    31681 aagataaagc caagattgta gaggacagta tttcgtgtga taataaaggc gtctcttgaa
    31741 ctaaataaga atctaaaatt atcgagtccg acccatttac tatttatgat actatctatg
    31801 aaaccattac tggtcatgtg gtagtctttg aaggcaacca cgttcccaaa tactggaatg
    31861 taaaagaata gaatcaacca gagtgcccct ggcaaaacca tcaagagaaa gatccagttg
    31921 tctctcaatg tttttgaaaa ctttttcata atttcctccc tttttatttt gatatccatc
    31981 taaaaattct tttttagact tttgataacg attacattat tagtatactc ctatttgcag
    32041 gttaggttaa actcctaatt atagaaaaaa ctccacaaat tatgtagcag atttaaaact
    32101 ttatcaccac tatcaaacaa atgtcctaaa tcaattgttt attttatctc tattagccca
    32161 gtgatggcgt cactctgtta taagcatcca acaacggggt atactgaaaa atctccagac
    32221 tagggaactc agcgatagtt cctaatctgg agatttttaa tatgttatta ggcgtttgct
    32281 ttcaacttag caataacctc tttaagatta tcaatcaact ctgctgcagt atgctcagag
    32341 cctttttcat ctgccaagaa caaaactgct ttttgaagtt ctttttgaga gttttcaagg
    32401 acatccttat ctactgtttc aaggtttgag tctttaagaa gtttacttaa ttccttggct
    32461 aatttcttga gtttgatttg cagactcatc ttctcctgct gtttctttgc ccgctgtttg
    32521 tcctccatcc ttagttgctg actggctttc cttaatggac tctagggaag caatggcatc
    32581 tttgactgtt tgcaagatat cacgtaaacc ttgctctgtc aaactatcat ctgtaagagc
    32641 tttcttagcc tctgccaaaa ccagacgtgc tgaatctgtg gtaggattcg atacacctgt
    32701 caatgatctc aaaagatttt ctaaggtttg agtctgctta ctaatactag actaaaatca
    32761 aaaagcatta cataacagtg atatgaaatc aactaaagaa gaaatccaaa ccatcaaaac
    32821 acttttaaaa gactctcgta cagctaaata tcataaacgc cttcaaatcg ttctatagta
    32881 aaatgaaaca agaacaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt
    32941 tagaagtaga tgtgtactgt tctagtttca atccattata tttcgtctga tgggcaaatc
    33001 ttataaagag attatagaac ttttatagta gtttgaaata agatgtgaac aactctatca
    33061 ggaaagtcaa attaatttat agaaatattt tagcagccaa ggtgtactgt tatagattca
    33121 atacact
//
LOCUS       DAVKFH010000022        32652 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKFH010000022 DAVKFH010000000
VERSION     DAVKFH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.022"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(325..1014)
                     /gene="rplA"
                     /locus_tag="VW006_001074"
     CDS             complement(325..1014)
                     /gene="rplA"
                     /locus_tag="VW006_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9379940.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1222..1647)
                     /gene="rplK"
                     /locus_tag="VW006_001075"
     CDS             complement(1222..1647)
                     /gene="rplK"
                     /locus_tag="VW006_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9379941.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(1770..2486)
                     /locus_tag="VW006_001076"
     CDS             complement(1770..2486)
                     /locus_tag="VW006_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9379942.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2504..2965)
                     /locus_tag="VW006_001077"
     CDS             complement(2504..2965)
                     /locus_tag="VW006_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9379943.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(2967..4004)
                     /locus_tag="VW006_001078"
     CDS             complement(2967..4004)
                     /locus_tag="VW006_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9379944.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(4102..5427)
                     /gene="brnQ"
                     /locus_tag="VW006_001079"
     CDS             complement(4102..5427)
                     /gene="brnQ"
                     /locus_tag="VW006_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9379945.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5598..5690)
                     /locus_tag="VW006_001080"
     CDS             complement(5598..5690)
                     /locus_tag="VW006_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9379946.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5733..5885)
                     /locus_tag="VW006_001081"
     CDS             complement(5733..5885)
                     /locus_tag="VW006_001081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9379947.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(6045..6623)
                     /locus_tag="VW006_001082"
     CDS             complement(6045..6623)
                     /locus_tag="VW006_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9379948.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6677..8077)
                     /gene="pepV"
                     /locus_tag="VW006_001083"
     CDS             complement(6677..8077)
                     /gene="pepV"
                     /locus_tag="VW006_001083"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9379949.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8090..8695)
                     /locus_tag="VW006_001084"
     CDS             complement(8090..8695)
                     /locus_tag="VW006_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9379950.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            8866..9666
                     /locus_tag="VW006_001085"
     CDS             8866..9666
                     /locus_tag="VW006_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9379951.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9850..10683)
                     /locus_tag="VW006_001086"
                     /pseudo
     CDS             complement(9850..10683)
                     /locus_tag="VW006_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(10673..12520)
                     /gene="uvrC"
                     /locus_tag="VW006_001087"
     CDS             complement(10673..12520)
                     /gene="uvrC"
                     /locus_tag="VW006_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9379952.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGSIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12560..13261)
                     /locus_tag="VW006_001088"
     CDS             complement(12560..13261)
                     /locus_tag="VW006_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9379953.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLAGQ
                     FLSVTVRLNHYYLSNPHYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13340..14572)
                     /gene="murN"
                     /locus_tag="VW006_001089"
     CDS             complement(13340..14572)
                     /gene="murN"
                     /locus_tag="VW006_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9379954.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEYFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14577..15797)
                     /gene="murM"
                     /locus_tag="VW006_001090"
     CDS             complement(14577..15797)
                     /gene="murM"
                     /locus_tag="VW006_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9379955.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15909..16688)
                     /locus_tag="VW006_001091"
     CDS             complement(15909..16688)
                     /locus_tag="VW006_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9379956.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16757..18418)
                     /locus_tag="VW006_001092"
     CDS             complement(16757..18418)
                     /locus_tag="VW006_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9379957.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18593..20815)
                     /gene="recJ"
                     /locus_tag="VW006_001093"
     CDS             complement(18593..20815)
                     /gene="recJ"
                     /locus_tag="VW006_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9379958.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GGASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21092..21895
                     /locus_tag="VW006_001094"
     CDS             21092..21895
                     /locus_tag="VW006_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9379959.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     EVADRIIFMADGEVLVDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            21935..22699
                     /locus_tag="VW006_001095"
     CDS             21935..22699
                     /locus_tag="VW006_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9379960.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     EFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22712..23389
                     /locus_tag="VW006_001096"
     CDS             22712..23389
                     /locus_tag="VW006_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9379961.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23399..24058
                     /locus_tag="VW006_001097"
     CDS             23399..24058
                     /locus_tag="VW006_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9379962.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNSVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24354..25544
                     /locus_tag="VW006_001098"
     CDS             24354..25544
                     /locus_tag="VW006_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9379963.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25865..26746)
                     /locus_tag="VW006_001099"
     CDS             complement(25865..26746)
                     /locus_tag="VW006_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9379964.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26887..28215)
                     /gene="vncS"
                     /locus_tag="VW006_001100"
     CDS             complement(26887..28215)
                     /gene="vncS"
                     /locus_tag="VW006_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9379965.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(28212..28868)
                     /gene="vncR"
                     /locus_tag="VW006_001101"
     CDS             complement(28212..28868)
                     /gene="vncR"
                     /locus_tag="VW006_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9379966.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28917..29000)
                     /gene="pep27"
                     /locus_tag="VW006_001102"
     CDS             complement(28917..29000)
                     /gene="pep27"
                     /locus_tag="VW006_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9379967.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(29180..30559)
                     /gene="vex3"
                     /locus_tag="VW006_001103"
     CDS             complement(29180..30559)
                     /gene="vex3"
                     /locus_tag="VW006_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9379968.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30611..31258)
                     /gene="vex2"
                     /locus_tag="VW006_001104"
     CDS             complement(30611..31258)
                     /gene="vex2"
                     /locus_tag="VW006_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9379969.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31271..32548)
                     /locus_tag="VW006_001105"
     CDS             complement(31271..32548)
                     /locus_tag="VW006_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9379970.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGNSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9203 a         7487 c         6095 g         9867 t
ORIGIN      
        1 attgttagaa atcgatttga ctgtcctgaa cgattcgtcc tattcttatt tcattttatt
       61 ataattgata gtggtcgccc cagccagata ccttatccgc tatataggcc ttattatcac
      121 gttcctgttc gtagagccac gatttcgcta tcccttcctc tgacgattct tatttccagc
      181 gtcagacttg ggaatcgcta ttgggttcac cggtagtagg tgcccaccga ggacttacac
      241 ctcagatata cgacatgccc gtcgtacatc aaaaaaagtt gagacatagt gtcttcaact
      301 tttttacatt actttaacta ctgattaaag tgagtttacg tcaactttga taccaacacc
      361 ttgagtagtt gtgatagtca agtttgttac gtaagttcct ttagctgtag ctggttttac
      421 tttttggatt gtttcgttga aagctttaaa gttttcaacc aatttttcag cttcaaatga
      481 tactttaccg atgattgctt gaacgttacc tgcacggtca gcacggtaag tgattttacc
      541 acctttagac tcttcaactg ctttagcaac atccattgtt acagtaccag ttttagggtt
      601 tggcatcaag ttacgtggtc caaggacacg tccaagacgt ccaacaagag ccatcatatc
      661 aggtgtagcg ataactacgt cgaagtccaa ccaaccgtcg ttgattttag caacaaggtc
      721 atcttcacca acaaagtctg caccagcagc ttttgcttct tcagcttttg caccacgtgc
      781 gaaaacaaga acacgtgaag ttttaccagt accgtttggc aataccattg ctccacggat
      841 ttgttggtca gcttttttaa cgtcgatgtt caagttgtaa gcaacttcta cagttgcatc
      901 aaattttgca aagttagttt cttttgcaag tgctacagct tcttctacac tgtatgcttt
      961 tgtgctgtcg attttctcaa gagcagcacg aagttgtttg ctttttttag ccattttcta
     1021 ttctccttgt aagtggttca atcgattttc atctcccacg tcacttctcg aaatgatgaa
     1081 gtcttgcggg ttttcagtct actatttttc tcttcctacg gccttaagct cccttgctgt
     1141 atccaagtac aagcctcagt cacttgccta gtatgaaaag caactaatag actgctgtga
     1201 gattttctac tgtgttattg attagtcaac aacagtgaat cccatagaac gagcagtacc
     1261 ttcgatcata cgcattgcag actctacgtt tgctgcgttc aaatctggca tcttagtttc
     1321 tgcaatttct tgtacttgtg cacgagtaac tgtagcaact ttagttttat taggtgtacc
     1381 tgatcctttt tcaacacctg cagctttttt caaaagaaca gcagctggtg gtgttttcgt
     1441 aacgaaagta aatgatttat cttcgtaaac tgagataaca actggaatga tcataccagc
     1501 ttggtcagct gtacgagcgt tgaactcttt tgtgaatccc atgatgttga taccagcttg
     1561 accaagagca ggtccaaccg gtggagctgg tgtagcttta ccagcaggga tttgcaattt
     1621 tacaagtttt tcgacttttt tagccatttt taaatcctcc tttgtggttt tggcggtaat
     1681 tagagatttt tacctcccac aagtatgctt tacacatacc tttccattat acactattta
     1741 tctaaaaatg caagagaaaa ctttcatttt taatcgacat aatcgccgcc ttcaaagcca
     1801 tagtactctt ccaaactgag accacttgca gcaatttctt tggcttcttt tccaacgtag
     1861 cggagatgcc attcttctgc catgtagcca gtctcctttt ccttgccttt aagatagcga
     1921 acaacaaagc catagtcagc cgcatggtcc aagagccact gggctgcctt ttcttcagta
     1981 actaaatccc catctgtccc aatcaaatca aaagcaagac ccgtctggtg ttcactgtaa
     2041 cctgggcgtg ctgagtagcg atctgctgct tccttaccat cttgattgac atagtcttgg
     2101 tagagcttag cctgagtttc ataactccta aacccactgt attggtcact gattggatag
     2161 ccctcagcct gcatagctgc gattagtttg agcaactctg ccttggctgt tggattttct
     2221 cctggattgt aatcttttga caaaggataa cgtttattag ctaggataat ctcatcgtat
     2281 ttcccttgga tactataata gctcccttta ttgctaactt ctgccttttt ctgactagaa
     2341 gcttgagatt tgctacctac agttccttcc ggttgactgc tttgctccgt tttttgagtg
     2401 ttttcttcaa ctttggtttt ttcttgcgaa caagctgcaa gggtcaatgc tagtaaacca
     2461 gataaaatga catatctttt tttcatttct ttcttcctct ctcttaggcc aaatacttct
     2521 ccaattcatg agataagatt ttaatcatct cttccagctc tggcgattgt acttggcaat
     2581 cttctgccgc catttcttcc cagggcaccc accagactgg gccacggcca ttcaaaccgt
     2641 gactcttcat atcacctgct cttcggggaa aaagatgcca atgagcgtgg gcatcgccat
     2701 ttcctaggag ttcaatattc atcttttcag cctcaaatgc tttggcgact gcttcttgga
     2761 ctaaactcat ttcctctaaa aagcgaagtt ttacagaagt ttccatatga tggagttctg
     2821 tgacatgatc ttttgctaaa aataaggtat agcccttaaa gtattggtag tcaccaataa
     2881 caacatagcc tgtttctagt tcttttacaa agtagggatt ttgcccagct ttaatgagtt
     2941 cgattctctg acagattaag cacattctat gataccgcct cgctcttaag ataagcatca
     3001 accatacgct cagtcgctat tactgaatcg atgtgggtgc gctcataaga gtgactggac
     3061 tcaataccag ctccaagaag tgcatgcttg acctctgcac ctgctgacat cgctgctgaa
     3121 gcgtccgatc cgtaaaatgg atagatgtcg agcttaaatg gaatatcttg ctctttagcc
     3181 aaggcaacca agtgttgacg gaagtcatag tgataaggac ctgaagcatc cttgacacag
     3241 atagatactg tgtactcatc tgtctgctgg tcatctccca tagctcccat atccacagcc
     3301 agatattcta ctacctgagc aggaatgtta gaattagcac catgtcctac ttcttcaaaa
     3361 actgaaaaag caaaatgagt tgttactggc aattcaatct tctctttctt ataaatacga
     3421 agcaggttga gcaaaatcgc tgcgctgacc ttatcatcca aatgacgaga cttgataaaa
     3481 cctgtatcag tcacaacagt tcgtgtatcg aagctgataa aatcaccgac ctcaatccca
     3541 agagcacgag tttctttttc attgcttact tttacatcca aacgcacttc catattgtcc
     3601 tgcgtgcgtt ctgcagttcc tgcatccttg tagacatggc aagaagtttg gtggatgagg
     3661 atggttcctg ataccttttg acctgtgcta gccacatgaa cggtacagtt ttcaccttca
     3721 atcatgttcc aaggaaaacc accgatacgg tccaatttaa gacgaccatc tggtttgaca
     3781 gcacgaacaa tagcacccag cgtatccaca tgggcagtca catagcgatg ttgctcatca
     3841 ttttgacctt tgatggtcac attgacacca cccttagctg tgcgaactgg ctggtaacca
     3901 aaaccttcta gagtcttgac taaataattc gaaatttcac gagtgaagcc cgttggcgat
     3961 gcgatggctg tcagttcttt gatatattct actgtttgat tcatttcttc acctctttcg
     4021 ctacctatta taacacattt atcatcggat aaaaaaagaa aatcactcct gtagacttgg
     4081 ctacaaaagt gatttaagtg attattccat ttcaaaaaca tcgctttctt gcttgtttgg
     4141 tagaaccaat gagagcaaga ttccaacaat ggctggcacc aaccatggga gagatgcctt
     4201 ggcaaaagga agaacgctaa caagatttgc aagaaactca accttaaacg agcttcctag
     4261 tacgcttgca atggcaataa ctgtaaccac agcaattgtc aactgcatac ctggttttga
     4321 aagggcaaca aatttgttga caatgacaat cataacgata gcaatcgtga ttgggtacaa
     4381 gataaccagt actggaattg agtacttgat aatcgcatca agacccaaat tggcaatagc
     4441 aaatccaatc aaggtaaagg ctgtcgcata aaccttgtag ctgatttgtg ggaagcgctc
     4501 attaaagaac tcagctgttg acacaatcaa accaacagtc gttgtgaagc aggttacggt
     4561 aaccatagct gcaaggaaga gttgagctgt tgagccaaag atttcttgag tggcttgtga
     4621 caagatgtaa acacctggtg ttccaccctt catcgcttca gctggtactg ggaaatgatt
     4681 tccaagaaaa cctaaaccga tgtaaagagc gctgaaggca agggcaacaa cgataccaac
     4741 aacccaaata gttgaaatgt attctttctt acttgaaaat ccaagttgtt tcaaggtttg
     4801 aactgcgatt acgctaaagg ccactgaagc aagggcgtcc aaggtattgt aaccttctag
     4861 gaaacctgta ccaaaggcag aagcttgata agcagctgaa gcagcttgag gacttgttcc
     4921 accatatttg atagctccca gaacgaccaa gataacaatc aaaattgcaa agactggcgt
     4981 taaaacacgt ccaatgcggt ctaagatttt tgatggatta agcgaaatca aataggctgc
     5041 cgcaaaatac agaaccgtaa atacaatcaa gccaagtcct ttatttgcat ccgacaaaag
     5101 ggggctaatc cctacttcgt aagctgttgt agcagtacgt ggggtagcaa agaatggacc
     5161 gattgacaag taaagaactg agaggtaaag agtcgcaaac caaggcgcta tcttcgttga
     5221 aatctcgtag atatatcctt taggatttag cgttccaata ataagggtca agacggcgat
     5281 accaacgcct gaaaagacaa aacctgcgat ggcaggaaga aaatgttctc cagatagagc
     5341 acctagagaa ggcggaaaaa tcaagttccc cgcaccaaaa aatattccaa acaggagcaa
     5401 acctgttagg gtaccttttt tagccataaa aatctccttc atatttataa aatactgacc
     5461 tagtatacca cgattaggag tctgaaaaaa gcatcacgag gcaaatatta aatgttttca
     5521 attttcttaa aaataagaat tatctaaaaa taaaactctc ctacccagtc cacaccaagc
     5581 aggagaaatg caaaatttta aagttcttca aaggctaaca tgccaccctg gacattgata
     5641 acattataac cttgttctaa caggaattgg caagcacgcg ccgatctcat tccagatttg
     5701 caaataatat aatgcaagcg atctttgtcc aattaatcat aactatcagc caattgacta
     5761 agcggtaggt tgtgggcaca ttctaaatga agagctgcaa actcatccac ttctctcaca
     5821 tccactagag aaagctggtc gttttggtaa agctggtaaa atgcgtcaaa ggctatttct
     5881 ttcattcttt ttcttctaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt
     5941 accgtgcgta tgttaccgac ttcgtcagtt ttatctgcaa cctcaaagct gtactttgag
     6001 cagcctacga ctagcttcct agtttgctct ttgattttca ttgactataa aatggtttta
     6061 attctttttt tcaaatctgg cactacttct gcctcaaacc aaggattttt ggccatccag
     6121 atttgatttc gtggtgatgg gtgaactagc ggaaaatagg ctggcagata gtctttatag
     6181 tgtttcaccc tctccgttac cttcccactg attttctcct gtaaatagta ggcttgggca
     6241 tattgcccaa tcaagagggt taactgaata tcaggcaatt cctgtaagag ctgcggatgc
     6301 catttttctg caaaacctgt acgaggcgga agatcacccg acttgccatg tcctggaaag
     6361 tagaaatcca taggcaaaac agcaaaataa cctgaattgt aaaaggtatc ttcatccaca
     6421 cctagccagt cccgcaagcg gtcaccactt ttatctttcc agtaaaggcc tgcttcttga
     6481 gttttaagtc ccggagcctg accgatgata ttgatgcgag cagtttttgg cgctgcaaag
     6541 agaggctcaa tgccacgctc tgtatagctg gcattctgtg aatccgccat gatagcctgt
     6601 ttgattcttt cgatttgaga catctacttt ccctccaaaa agaagtccaa gcataagatc
     6661 tcagacttct atcgttttat ttgatcaatt cgtaaatagc ttcggcatag attgctgctg
     6721 ctcggaagag atcgtccaag gcgataaatt cattggcttg gtgcatggta tcaattgagt
     6781 ctgggaacat agcaccatag gcaactccgc gctctaacaa gcgaccaaag gttccaccac
     6841 cgatgacttg ttcatgacct ttaaagccag tttgtttttc atagatattc aacaaggttt
     6901 gcacaagtgg atcttccatt ggcacatagt gaggcgtgtg accgtgttca gacaggctaa
     6961 cagaaacaac tggcaagttt tcaaggattg acttgatttg ttctggactt gttccttttg
     7021 gatagcggat gttgagggca atggtattat cagcacttgt ttcatcgaag cggaagacgc
     7081 cggcattcat agaaagagca cccatctttt catccacatg agcaatctta agattttcac
     7141 cctcatgatc gttcaagaga attttacctg cgatgtcaag gtagtctttg gctggaccag
     7201 caaagccaaa ctgactgagg aagagggcaa ggtaagttgc gccattgaca cctgaagcag
     7261 gcatagcacc gtgggctgat ttaccaatga tcgtcacctt gtatttgcca gcttcttctt
     7321 ggagttctcc tctaagtttg tgttctgcaa caaaggcatc tagtttagct tgcaagtcag
     7381 ccaagtcacc tgaaacgact gctgttgctg attctggtac catattttca cgtaaaccac
     7441 ctgtaaagct gtgaagacgg gcaacacctg tattttctcc tgcaaagtgg aggtattccg
     7501 tgatatttcc tttttcacca ttgatgattg gaaattcagc atctggtgag aaaccgaaat
     7561 ctggtttggc aagtcctacg tgctcaaagt agtagtccat gtctgcccag cctgattctt
     7621 cgtctgttcc aacgatgaag cgaactttct tagaagttgg aagacccaat tctttgatga
     7681 ttttcaaacc atagtaacaa gctgttgtag gacccttatc gtccgaagcc ccgcgcgcat
     7741 aaaggcgacc atctttgata gttggtgtgt aagggtctgt gtcccaaccg ctaccagcag
     7801 gcaccacatc catatgggca aagattccga gaacttcttc tccatcacca aactcaaaat
     7861 gtcctgcata gttatcaaca ttcttagttg ggtagccatc gcggtctgcg atttcaagga
     7921 atttctccaa ggcttttact ggaccaggcc caaatggatg ctgggcatca gccttgctgt
     7981 catcacgttc tgaattgatt tccaaaaggc taaacaagtc agccaagagg tcttctttgc
     8041 gtttttctac ttctgctgta aaatcaattg ctgtcatttt ttcttctttc tatcttttct
     8101 cgatgatttc atctactggc aagcggtagc ttggttccaa tttttcgtct gtataaccca
     8161 ctgtgatcaa gagttctggg cggaaacggt cttcgatttc caaaacttca ttaacttttg
     8221 atttgtcaaa accaagaata atgttagaac caattccttg gtctgtcaat gcaagaacca
     8281 agttcatggc aaccaaacct gcattgagag ctaggtagtc gctgacttgt tgttcattgt
     8341 agcgtgcaaa ttcagctggc aaatttttca tgaagtattg aagttgttct tctgagaaat
     8401 tgttagcacc accaactcgg gcaatcttac gagcgcgttt ggcaaggtct gtatctgtaa
     8461 atagggcaat ggttacaggt gctgatgata cctgctcaaa gtttgaacca taagccaact
     8521 ttgccagttc agcatttttc tcacgaacaa ctacaaattt ccaaggctgg ctgttgtggg
     8581 cgcttggcgc caaggttgca atttcgatag ccgtacgcac atcttttgga tcaacaggct
     8641 tatcagtgaa atgcttggtc gcatgacgtt ttttatttaa ttcaagaaat ttcataatcg
     8701 atttcctttt ctaattctac tgcttccatt ctaccataat ttgaaagagc ttgcagggat
     8761 ttttcatgat taagtttttc tatcacagac ataaaagata tatattggtt tatcaggaaa
     8821 gtttctgata aactagcaag tactttacat ttgaatggag acagtatgaa aaaatttagc
     8881 ctattactag ctatcctacc atttttggtt gcctgtggga atcaagctac acccaaagag
     8941 actagcgctc aaaagacaat cgtccttgct acagctggcg acgtgccacc atttgactac
     9001 gaagataaag gcaatctgac aggcttcgat atcgaagttc taaaggcagt agatgaaaaa
     9061 ctcagcgact acgagattca atttcaaaga accgcctggg aaagtatctt cccaggactt
     9121 gattctggtc actatcaggc tgcggccaat aatttgagtt acacaaaaga gcgtgctgaa
     9181 aaataccttt actcgcttcc gatttccaac aatcccctcg tccttgtcag caacaagaaa
     9241 aatcctttga cttctcttga ccagatcgct ggtaaaacaa cacaagagga taccggaact
     9301 tctaacgctc aattcatcaa taactggaat cagaaacaca ctgataatcc cgctacaatt
     9361 aatttttctg gtgaggatat tggtaaacga atcctagacc ttgctaacgg agagtttgat
     9421 ttcctagttt ttgacaaggt atccgttcaa aagattatca aggaccgtgg tttagacctc
     9481 tcagtcgttg atttaccttc tgcagatagc cccagcaatt atatcatttt ctcaagcgac
     9541 caaaaagagt ttaaagagca atttgataaa gcgctcaaag aactctatca agacggaacc
     9601 cttgaaaaac tcagcaatac ctatctaggt ggttcttacc tcccagatca atctcagtta
     9661 caataacact cttcgaaaat ctcttcaaac cacgtcagct ttatctgcaa cctcaaagct
     9721 gtgctttgag caacctacgg ctagcttcct agtttgctct ttgattttca tattgagtat
     9781 aagatatatt aaaagcgatg ggatcagcca atcgcttttt tagtttgcat tggttatttt
     9841 aggaaactct tacaaaaaat caaaaaaatt cttcacatcc tctacatacc tatgcttttc
     9901 tattaagctt gctaatagtt ccagattgtg cctctgatag ttatcttcac gacgtagttc
     9961 ttcatacatt tcgataaagg gaaaaccctt ggatttggca aattctaact cagcctcgta
    10021 atcataagcg actttacgaa ataggatatt taccaattcc ccatcttcaa cctctatcac
    10081 ggcatactgg gcacggtgat tttttaacgc ctcccaatta aaatagggca tgccaatcga
    10141 ccctggattg atgatttgtt gcccttgact accataacga agcaactact tgtgaacatg
    10201 accataaact gccacgtccg tttccgcatc tagcagttgg tcaaatttct ctgtatcatt
    10261 ctcaactagc aagtcaccac catagttttt gtcaggtaaa ttatgagaga tagaaaagcg
    10321 caatccgtca atttctttct tttccagcaa aggcaagctt cgtagccaga caatcgttgc
    10381 aggatccatt cgctccatca aatactgtgt catacgcaag agctgaactt cctgtgggtc
    10441 ttctaagcca tattgcccat ctaaagcctc aaggacacaa tcatcccaat tgcctcgaac
    10501 acttgccgtg ataggaagtt cctttaacag agcgaccaag tcatttgcac ctggaccagg
    10561 gaggaaaatg tctcccatga gccaatattc actggctcct tgagttttag catctgcaat
    10621 cactgcttct agcgccgtcg cattgccatg aacatctgat aaaattgcga ttttatggtt
    10681 tattgtggtt tccttccgtt tggtaatcta ctctttcttc cgccacttga agcaagattt
    10741 ctgtttcctg cgggttcaac ttggtttgca cagcctctgc tactgctctt ggtactccaa
    10801 cttcgacaat ctcatccaca ctggcttcct tgattttggt cagagacttg aagtgcctca
    10861 tgagattttg cttgcgttta ggtcccagac catcaatccc atccaattga gatgagaaag
    10921 aatttttgga gcgcagttgg cggtggaagg taatggcaaa gcggtggacc tcatcttgaa
    10981 tgcgttggag gaggaaaaat tcctgagaat tgcgagacaa gtccaccact tccagcggat
    11041 ctccaaagag caattcatgg gtttggtgct tatcattctt ttgcagacct gcaattggaa
    11101 tatccaagcc cagttcttct tggatgactt gcttagcaat attgacttga ccttgccccc
    11161 catcaatcac aatcaaatct ggaggagtca aagcctcacg ctgtactcga ccatagcgtc
    11221 tgcgaatgac ctctcgcata ctggcatagt cgtctggccc gactacggtc ttgattttat
    11281 acttacggta atctttttta cttggcctgc cgttaacaaa gaccaccatg gctgaaacag
    11341 gactggtccc catgatgttg gagttatcga aggactcgat gcgaactgga gtcggaattt
    11401 ggagcaaacg tcctagattt tcaatagctc cttgggtctt ttcgactgat ttttctagca
    11461 ggttaaattt ctgctccaga ctaacacgtg cattttttat ggctagattg accagctgtt
    11521 ttttctctcc acgttggggt ttgagaatct tggtatccac caaagccttg acagcttctt
    11581 cgtcaatatc ctgcggaatc agtacctcat tgggaactag gtgagatttt tcttgataga
    11641 attgtcccac ataggtcaag aaatcctcat ctggatcatt gaagtagggg aagagattga
    11701 catcgcgctc gatgagcttg ccctgacgaa caaagaaaac ctgcacacac atccagccct
    11761 tatccacata gtagccaaag acatcgcgat tttggagatc cttagccatg acccgttgct
    11821 tggttcgaag cgttccaata gcctgaatca ggtcacggta ttccgccgca cgttcaaact
    11881 ccatagtctg ggctgccgct gccattttgc cctttagctc atcaatgatt ttgttatcct
    11941 gccctttcag aaaatcagaa acctcctggg ccatagactt gaaataagcc tcatccttct
    12001 tacagatggt gtgagccata cactggccga tatggtaata aaaacagacc ttagagggcg
    12061 ggttggtaca cttacgaaaa gggaatatcc gatccagcaa ccgcttgatt tcattggctg
    12121 cccccacatc gggatagggt ccaaaataaa gacctccgtc ctttttgacc tgacgagtga
    12181 taatcaagcg tggatagcgc tcattggtga ttttgatgaa aggataggac ttgtcatcct
    12241 tgagcatgat attgtacttg ggcttgtttt ccttgatcag gttgatttct aggagaagtg
    12301 cctcaatatt agactccgta acaataaatt caaaatccac aatttcagac accagagcct
    12361 ctgtcttggt atcatgactt ccacgaaaat aggaccgtac tcgattacgc agatttttag
    12421 cctttcctac atagataatg gagccatttt tatccttatg aatgtagcaa ccagggctgg
    12481 tcggcaagag ctctagtttt gatttaatca agttgttcat agttccatta tagcaaaaaa
    12541 agtagggaaa gatagtaccc tactcactag tctcatatat ttttcggagg tttgcaacat
    12601 cctcttcctg gataccataa aaggaacctg ctggttgcat gacagacttc tcatctgtat
    12661 ggtccaagcc aatcgcatga cctaactcat gttctgccgt atggacaagg cgttcatagg
    12721 agtagccata gtgtggattg gacaaataat aatgattcaa ccgcaccgtt acggacaaga
    12781 attgccctgc taagagatta gtttgacttt ccgcctctcc tgccacagga gtgcctccgt
    12841 cgttcatctc cgtagccgta atatccgcct tactagactc agtcacgagt tcaaagttaa
    12901 aagcaccagt ttgattccag ttctggattg cttctaaata agcctcttga aagcgtgaat
    12961 ccatctgcgg atccaggtaa atacgagcag aggcctgtgc ccaccttcct tcagaatgct
    13021 ggtgactatc tgtctggctt aacttaggca gttgccctgt tttctcccat tggtcaatac
    13081 tttgttgacc aatttttact gaccgttctg cttgctttag cgctccttga aaatccccgt
    13141 tcagatacca gagaagtccg aaagcaagca cacataagag cacaactatc caaaccagac
    13201 gccaaaacaa acgccacaca aaagaaaaga aagcccctat caaacgaaaa agccagcgca
    13261 tgtctctcca cctttctagc aaataagaat tattttacta aaagaagcta aaagaaatct
    13321 aaatactgac tttttcatct taacgtccta ctattttttt gagtaactgg atagctttgt
    13381 attttaaagg cgatggatgg taacggaaag tacccgcttt gcgtacaata tagccattaa
    13441 aattctgttt aaaacgcaaa acaccatcac ttccatcaaa aatcccttga atgcctagga
    13501 aattgtattt aggtattcca cgttttatgc tttccaacat aacatatttt tgaagcagtg
    13561 caggggcata gaacttatta aactcagtgt aggaaccact aaagagataa gtcgtttcct
    13621 gaggcatata aacaaataaa ctcccagcta aaacaatatc ttcttctcca tatttttcaa
    13681 tcaagtctcg cgcttctgct tttcgaactt caaacgtttc aaattgacta gaatattctc
    13741 tcagttgatt ttgttttttc tcagaatgag gatttttact caaatcaagt cgcaacttgt
    13801 ccaagttttc ttctagttta ctttgttcac cttgcaattt gctcatatac tccgaaaaat
    13861 tcaagcttgc tatgagaaac tccgcttgtt ctccaaaagt atcataaaaa tactcataat
    13921 attctaaact tttatcacta tattctctac gttcagaggt ttcttttgtt atattcttaa
    13981 aaatcgatag ttcttcacgt tttaactttt tcaaccgaat gccaaaggtt tcagcctttt
    14041 tcaccaaggg tttacccttt ttgctaaaac ttttaagcaa actcttttca gttaattcag
    14101 ttaaatcttt atagtataac caatctggtt ctccacctgg gtaacctgtt gttaagccat
    14161 caaattgata acctaaatca gtcaaacctt gaataatact ttttttctca gcatctattg
    14221 gattaccttg gctatcaaaa gtttgataag tttcataagg ttttacaagc aactctaata
    14281 caccattttg cttggcatat tcttttaact ctgcataaaa aactggaaga gcatcttgtt
    14341 gggtataaat cggccccgaa ttgagttcca tatgcagacc acccagcatg ggcaggctat
    14401 aaaccagagc tgcaacttga atttctcctt cttgtttcaa agcaagataa acaattcgag
    14461 cccctctttt ttctagcaaa tcccccatct ggacagattg cataaaggaa cgagaagaaa
    14521 cctgatcaga ataagtctga aactcttctt tcgtgagtgt tgttagtgcc atatacttac
    14581 tttctatgtt tttttcttaa tgttttacgg aaatcaagag caagtcttaa cagaggatag
    14641 agaggatgag tgggcattgt aaattcaccc aagtattctt caatcgttgg attaaatttt
    14701 tccttaaaat gataaagtcc accattgaga gagttttcaa caccacctaa attttgccag
    14761 accatacctc gctcaaaggc atagcgagcc gtttcatacc atgttaaaat tggtgcattg
    14821 taacgtttaa aatcatcatc cataccagca tatatattga cagaggtagt accaaattcc
    14881 aaactcaaag tagccgctaa aggaactctc gcttgaccta catctatata ttcctgcaag
    14941 aaggtcaatt cctctaacaa acgttctttt tccttcttct gcgcttctac ttttgaagtt
    15001 cgagtcgact cagtaaacgt ctcttccaag gctctatttt tcgctaactg ttcttctaac
    15061 tcttgcgaac gtttagaaac atccaaggtg gccaaggtga tataggcctt gtccttaaaa
    15121 ttatctaaca attttttata ataggcttca ttcctcaaat gaatctcttt tcgcttctca
    15181 gtttttttca tcaactccga aaatgaatct aatagttcca gtccaccata ttgaatctca
    15241 agccctttgt ttcgtgctgt tcgaatagcc tgttttgttg acttggaaag tttatcttct
    15301 tcaaaatttt ccttgtatat tttcgcctga atacgaggtt gaatggtgtc tcccatttcc
    15361 tccgtttttc ctgaccacct tactcccatt tgttgcaaac tatcaataat agccagattt
    15421 tcaggaaatt ctgtcttttc ctgattgatt aaactttgag ataggcaaat acttgggtca
    15481 aaagtcacaa aaaccgctct cttactgcga gcataggact taatagactg aatgacaaaa
    15541 ttcaagagtt ctttatcccc ataatccaat ataggtcctc ttgggatgta aaacatttta
    15601 tagcctagcg gaagagttct aatcaaaata ctagctgtcg ccagtaattt ttcttccctg
    15661 taaacaccaa acttctcatg ttgccaatta gacttaactt cctcccaagc actactttgt
    15721 aatacattgg ctaattcatg ttctttgaca aactgatcat attctaatgt gggaatgcca
    15781 atttgataac ggtacatttc ctactctctt tcctccagta ttttattact attctactac
    15841 tttcgtttga aaagtctagt gaaaacatat tttattttag ttctactccc cctatgctga
    15901 agttcaaact aagtcagtct ctcttctaat ttgaaatcaa ttggtagggt tgttaaaaaa
    15961 acttccaatt gtttttccca gtagtaccac tcgtgagttc cagcgctatg gctataggtc
    16021 acatcaaaac ctagtttttt gagatttttc actgcgagat tattggcttc gtacaagaaa
    16081 tcctgttcgc cacaccacgc ccaaagtttg gtctttttat ccgatttttt agccagactt
    16141 tcaagagaat agggactagt tgtccagtct ttaatctctc caaaaacacc tctccagtag
    16201 gctggacttc ccagattttg actttcagga gaaaagtttt gaaagctgag ggcacctgaa
    16261 aagctagctg catgagaaaa acgatttgtc gcaagagcca gtttgaagca gccgtagcct
    16321 cccatagaaa gaccagcgat aaaggtcttt tcacgcttgc tagtcatatt agggaagaag
    16381 cgtttcagaa cctgtggcaa ttcctctgct agagccgtgt agtagtcaaa accatactgg
    16441 gtatcggtgt accaaccatt gctggtattg ggcataacaa cgatgagatt agttcctcga
    16501 agcaagcgtt ctacattggt ccgcttaagc caactattat gatttccaga catcccgtgc
    16561 aaaaggtaca agacgggaat atcttcacac tctggttctt ccactcgatt ggcatcaggg
    16621 tagaggacat tcacccccca ctccatatcc aatacttgtg agtaatactc gattttcatt
    16681 actgccatga ttttctcctt ctatttttct ataagaaaaa gccgaaactc gactttctct
    16741 ctgtttattt caacgattat tttgcttcca tgactactgg taaaatagct ggacgacgct
    16801 tggtttggtc aaagagatac ttggtcaaat tatctcgaac cttcccttta agatctgccc
    16861 agtcaaagtc atctccttga agatactctt ctaccgtttg gttaatcaat tctgaacttt
    16921 cacggagaat atcgcgactc ttcttgagat aaacaaatcc gcgcgtatga acacgagccc
    16981 tagccacaat tttcttctca cgacggttga ctgtaatagc cacgatgaaa attccatcct
    17041 ctgacaagac cttacggtca cgaagaacaa catttccaac atcaccaatg gcattcccat
    17101 caatcaagat atctcctgct gaaaccgatc cagctggaac aaagtctcca ttctcgtaag
    17161 ccatggtcgt ccccttttta ggaatgaaga tgtgttctgg caacatccca actgccatgg
    17221 cagccttagc gtgagcatcc aactcacgat actccccttg gacagggaag aggtacttag
    17281 gttgcaaaag attgatcatc agctgcaaat cacgcacatt tccgtgccct gatacatgta
    17341 aactttgggt aatcaatttg acaaccccac ctgcctgata aatcatattt tccacacgcg
    17401 caacaaaggc ttctttagca atagacggag ccgtagcaat atagaccagg tccccatcct
    17461 tgatttctac ataacgatgg cgaccaatcg acatcttacg aagtccattg ataggttcac
    17521 ccatacgacc tgtctcaaga ataatcaact catggtcttc aaagcgagac atatctttag
    17581 gcttaatcaa gagaatttcg ttggctaaag acaacttctt aagacgaatc gctgtgcgga
    17641 cgatattttc aatatcaaat cctgtcaaga cgatacgtcg acctgtttta tccgcagcgt
    17701 caaaaatctg ctgaatacga gaaagattac tggaaacagc tgcaacgatg atacgacctt
    17761 cccagtcagc aatagtttgg gtaatttcat ccctaacttc actttcacta gccacctgaa
    17821 tattgctgtc tgcattggcc gaatcactga ggagagccag gacgccgtca cgaccaatct
    17881 ctgccaaacg agcaaaatca gttgcataag attcactagc cgtttggtca aatttgaagt
    17941 cacctgtata aacgatgctt ccttccgatg tcttcaagac aattcccaga ctctctggaa
    18001 cggagtaagt cgtagggaag aaggaaacca ctgtcccacc aaaatcaatc tccgtattct
    18061 catcaatgac atggaaatca ttaaatttct taacggcatc atttcctttg acaaagagct
    18121 ttgccaactc aatggtcaac tcagacccaa atacaggaac tttagcctct gccaagagat
    18181 aaggtagagc accaatggca tccgcatgcc cgtgggtcaa gaaaacccca gcaatacggt
    18241 cgctattttc aaaaaggtaa tccatgtttg gaatcaccac atcgacccct aattgttcat
    18301 tttcaggata ttttaaccct acattcaaaa caaaaatgga ctctccaatt tcagcaatgt
    18361 acatattttt tccattctca cgcacaccac caagtgttgt taaactgata ttactcattt
    18421 ttcctccaaa atcttagttt atcttgatta tagggcaact ctaccctctt attctaaaag
    18481 ttctaatact ttcagccgaa tcttttccta ccattatacc atttttttga tgattttcaa
    18541 aatgaaggtc tgttttcaaa aaagagttga tttcccaact ctttcctaac ttctactctt
    18601 tttccatcaa aaaatcataa atttcttgca ccgtacccag cgccatcatt tcttggtctt
    18661 taacggtttg tttgagattt tggtaaattt gactttctcc tatctcccgc tttggcgcct
    18721 ctttattgac tgtcatcaca ccatctttta tcgtcacaaa gcctagttct tcaaatactt
    18781 gaatcatctt gaccagcaag atttgttgaa tattaagata tgcagccaaa tctttcagct
    18841 tgtagcgaat atcaaactct gggaactggt aaatagtctt gtacaatttg gcaaactgat
    18901 ctctagtccc ataacctgtc agataatagg ccttgtcaat atcatttttg aaatagacag
    18961 cagagaaatg ctgttcctga aaaatggtct tcagctgagt aatatcctct ggaatgtttt
    19021 ttacgacaat agcttcacta gccgcaagat ttggcagttc tccaggaaaa tccaagactg
    19081 gaacaccttc tggcaagact gcattttttc cacgaatgtt aaaaagttga acaccttcca
    19141 ctcgcgcatc caccatcatc aactggaggg cagtttggcc attccattgg ttgacagaca
    19201 atttaaccgc taactctaga ttcttggttt gagaaaactc tgtcgcccat ctgccttgac
    19261 caaaggctac cacttcaaaa ctcgccccac ccttggaaat tttcagcttt agatgggcat
    19321 tacctgcccc catagtacga gcactttcga cctgaaaatt cttgatataa aaaataggtt
    19381 tctgattatc cattccaaaa ggagctaaac gttcaaaact tttgaccgtt tccaagctaa
    19441 gtgcctccaa atccaactct tcatctaggt ttaacttatt cttgccacca gcatctgcac
    19501 ctttttcacg aacataatct tccaaaacct gagataaatc tgagagttgc tcaacttcca
    19561 gcgtcatacc cgctgcacct gcatgacctc caaaggcgat gaagaggtct cgatggggat
    19621 ccagagcttc aaaaatatcg accgcttcca cactacgagc actgcccttg gcacgaccgt
    19681 cttctatatt aagaacaatg actgtctgtc ccaattcttc caataaacga ccagccacga
    19741 ttcctagaac cccaggattc cagccttcct tggccaagac ctgaaccttc ttctcaggat
    19801 ccaccatggt cttggcttct tcatagatag actgaacgat ttccttgcgc tcttcgtttt
    19861 tctggtgaat cataagggca atctcatgcg cttcctcatc atcaaatcca gtcaacaaat
    19921 caatggcagg attgggatca tccaagcgac ccaaggcatt caaacgagga gcaatctgga
    19981 aaccaaccgt ttcttctgtt acttcgttgg cagcaatccc agccatgtcc agcatttctt
    20041 gcagaccaat gcgctgggta tgacccaaca tttccagacc atattgaact aagatacgat
    20101 tttcatccgt cagactcacc atatctgcaa tagttccaat agcgaccaaa tcaagcaatt
    20161 ccacttgcac ttcttctaac agggcacaag ccaacttgaa agcaactcca caaccagcca
    20221 aatatttaaa aggataatcc gcatctggat gttcaggatg gacaatagca taagcatctg
    20281 gcagggtttc aggcatggaa tgatggtctg tcacaatgac atctactccc atagactgag
    20341 ccaatgcaat agcctcatga ccagcaaccc cattgtccac cgtcacaatc aaggaaattc
    20401 cttcttgctc gataaagtat ttataaacac tagcattagg gccatagcca tcggtaaaac
    20461 gatttggcag gtaaactcgg cactccgcac caagttgttc caaactttcc ttcacaatag
    20521 aagccgaagt catgccatcc gcatcgtagt ctccataaac gagaatattt tccctttctt
    20581 caatagcctg acgaatccgc tccactgcct tgtccatatc atggagcaga taagcatcat
    20641 gtaagtcctc caaggaaggt tctaaaaact tcttcagact ttcttggtcc tgaatccctc
    20701 tctcaaacaa taaccgagcc acctcaggac ccagtccagc cttcttggct atctttgtaa
    20761 aatccgcatc ttctacctgc agggcaaact gccattcata agtaggtgtt atcaaaaaga
    20821 catccactcc ttctaagaat tctattgctt cattataaca tgatttagac gttttctcta
    20881 tccagattgc ttttttataa catttcatag tatattgaaa ctagaatagt acacctctac
    20941 ttctaaaata ttgttagaaa tcgatttgac tgtcccgatc gatttttcct gttcttattt
    21001 cactatagtg aattttttca gatatggttt gacaagaaca ttcttttatt gcagaattgt
    21061 gttataaaat ctaacataaa ggagttctct catggcttta gtagaattta aaaacgtcga
    21121 aaaatattac ggagactacc acgcactccg caacatcaat ctccgttttg aaaaaggaca
    21181 agttgttgtc ctgcttggac cttctggctc tgggaagtcc actcttatcc gtacgatcaa
    21241 tggtttagag gctgttgaca aaggaagtct cctagtcaat gggcaccaag ttgctggtgc
    21301 cagccagaaa gatttggtac ctcttcgcaa ggaagtcggc atggtttttc aacattttaa
    21361 cctttatcca cacaaaacag tgttagaaaa cgtgacactt gcacccatta aagttctagg
    21421 aattgataaa aaagaagctg aaaaaaccgc caaaaaatat ctggaatttg taaatatgtg
    21481 ggacaagaaa gattcctatc ccgccatgct atctggtgga caaaaacagc ggatcgccat
    21541 cgctcgtggt cttgctatgc atccggaact cctcctcttt gatgaaccaa catctgctct
    21601 tgatcctgag actatcggag atgttctagc agttatgcag aaactggcgc gtgatgggat
    21661 gaacatgatc atcgttaccc acgaaatggg ctttgctcga gaggttgcgg accgcattat
    21721 ctttatggcc gacggagaag ttttagaggt tgcggaccgc attatcttta tggccgacgg
    21781 agaagtttta gtagatacga cagatgtcga taactttttt gacaatccaa gcgaacctcg
    21841 tgcccaacaa ttcctcagca aaattatcaa ccacgaaagt gacaaagtca aataaggagg
    21901 cgcctatgaa aaaaaattct ttttatcagc attattgatt agccttttcg gccttgctgc
    21961 tgccaaacca gtccaggctg atacaagtat cgcagacatt caaaaaagag gcgaactggt
    22021 tgtcggtgtc aaacaagacg ttcccaattt tggttacaaa gatcccaaga ccggtactta
    22081 ttctggtatc gaaaccgact tggccaagat ggtagctgat gaactcaagg tcaagattcg
    22141 ctatgtgccg gttacagcac aaacccgcgg ccccctccta gacaatgaac agggcgatat
    22201 ggatatcgcg acctttacca tcacggacga acgcaaaaaa ctctacaact ttaccagtcc
    22261 ctactacaca gacgcttctg gatttttggt caataaatct gccaaaatca aaaagattga
    22321 ggacctaaac ggcaaaacca tcggagtcgc ccaaggttct atcacccaac gcctgattac
    22381 tgaactgggt aaaaagaaag gtctgaagtt taaattcgtc gaacttggtt cctacccaga
    22441 attgattact tcccttcacg ctcatcgtat cgatgccttt tccgttgacc gctctattct
    22501 atctggctac actagtaaac ggacagcact actagatgat agtttcaagc catctgacta
    22561 cggtattgtt accaagaaat caaatacaga gttcaacgac tatcttgata acttggttac
    22621 taaatggagc aaggatggta gtttgcagaa actttatgat cgttacaagc tcaaaccatc
    22681 tagccatact gcagattaag gaggacaccc catgacagat ttatcatctt ggacagccta
    22741 ttttcaggat tttggacaat ttttcaatgg tttcctcttc acccttgccc tagcgattgg
    22801 atcctttatc ctcgccatgg tcttaggaat cttctttggt gccatgtcaa ccagtaaacg
    22861 tccaatttta cgcattttag ctcgcatctt tgtggaattt taccaaaaca ctcccctctt
    22921 ggtgcagttt gttattgttt tttatggcct acctcttatc agtgaccata ctatcatgat
    22981 tccgatttat tggacagctg ttctctgcgt gggactctat cacggcgctt atatcgctga
    23041 ggttattcgt tcagggattc agtctattcc tagtggtcag atggaggccg ccttatcgca
    23101 aggttttacc tatatcagtg ccatgcgctt gattatcttg cctcagacct tccgcatcat
    23161 tctccctcca ttgaccaacc aaattgttaa cctcatcaag aacacctcta ctgtagctat
    23221 catctctgga gtagacttga tgtttgtgac taagtcttgg tcggctctca acggaaacta
    23281 tatcccagcc tttttaggtg ctgctcttct ctactttgcc ctatgcttcc ctgttgccca
    23341 gtttggtcgc aagatggagc aagccaacaa aaaagcctat tcactttagg aggttactat
    23401 ggaatctatt ttagaagttt taaccccaga taacctagtc tttatcttta aaggatttgg
    23461 cttgaccctc tatatttctc tgattgccat catcctctct actatcatcg gtacggtgct
    23521 agctgtcatg agaaatggca aaaattctgt cttgcgaatc atctccagta tttatatcga
    23581 gtttgtgcgc aacgttccca accttctctg gatttttact atctttttgg tgttcaaaat
    23641 gaaatccaca ccagcaggta ttacagcctt tactctcttt acatcagcag ccttggctga
    23701 gattattcga ggcggtctca atgccgtaga caagggacaa tacgaagcag gaatgtcaca
    23761 aggcttcacc tcagcccaaa tcctctacta catcattctc ccacaagcca tccgcaaaat
    23821 gctaccagcc atcatttctc agtttgttac cgtgattaag gataccagtc tcctctactc
    23881 tgttatcgcc ctacaagaac tctttggagc cagccaaatt ctcatgggcc gttatttcga
    23941 accagagcag gtcttcagtc tttacatcct gattgccctc atctacttca gctttaacct
    24001 agcaatttct agcctgtctc atatgctagc caaacgttgg caacaagccg cagaataata
    24061 ctcttcgaaa atctcttcaa accacgtcag cttcaccttg ccgtatatat gttactgact
    24121 tcgtcagttc tatccacaac ctcaaaacgg tgttttgagc agcctgcggg ctagcttcct
    24181 agtttgctct ttgattttca ttgagtataa acagccactc tccaattcaa ttggagagtt
    24241 ttttgatgag agtagatatt tatagacctc aatttgacac catacatttt ctatgataaa
    24301 atgtaacaaa ttcatttaca acaaatcgaa atctaaatga aatggaattt tctatgaaat
    24361 cagtcaaagg actactcttt atcatagcta gttttatctt gactcttttg acttggatga
    24421 acacttctcc ccaattcatg attccaggac tagctttaac aagcctatct ctgactttta
    24481 tcctagccac tcgtctccca ctactagaaa gctggtttca cggtttggag aaggtctaca
    24541 ccgtccacaa attcacagcc tttctctcca tcatcctact aatctttcat aactttagta
    24601 tgggcggttt gtggggctct cgcttagctg ctcagtttgg caatcttgcc atctatatct
    24661 ttgccagcat catccttgtc gcctatttag gcaaatacat ccaatacgaa gcttggcgat
    24721 ggattcaccg cttggtttac ctagcctata ttttaggact ctttcacatc tacatgataa
    24781 tgggcaatcg tctccttaca tttaatcttc taagttttct tgttggtagc tatgcccttt
    24841 taggcttact agctggtttt tatatcattt ttctatatca aaagatttcc ttcccctatc
    24901 tagggaaaat tacccatctc aaacgcttaa atcacgatac tagagaaatt caaatccatc
    24961 ttagcagacc tttcaactat caatcaggac aatttgcctt tctaaagatt ttccaagaag
    25021 gctttgaaag tgctccgcat cccttttcta tctcaggagg tcatggtcaa actctttact
    25081 ttactgttaa aaattcaggc gaccatacca ataatatcta tgataatctt caagccggca
    25141 gcaaagtaac cctagacaga gcttacggac acatgatcat agaagaagga cgagaaaatc
    25201 aggtttggat tgctggaggt attgggatca cccccttcat ctcttacatc cgtgaacatc
    25261 ctattttaga taaacaggtt cacttctact atagcttccg tggagatgaa aatgcagtct
    25321 acctagattt actccgtaac tatgctcaga aaaatcctaa ttttgaactc catctaatcg
    25381 acagtacgaa agacggctat cttaattttg aacaaaaaga agtgcccgaa catgcaaccg
    25441 tctatatgtg tggtcctatt tctatgatga aggcacttgc caaacagatt aagaaacaaa
    25501 atccaaaaac agagcttatt tacgaaggat tcaagttcaa ataagtaatc atactctcct
    25561 gggatttccc aggagagttt tttatagcca aatgaaataa gaccggaacg atggaatttt
    25621 ggcattcaaa tcaattgata agaatatttt atagtggagt gaaataggat acgaacaaat
    25681 tgattaggaa aatcaaatga atttatagaa atcttttagc agttatgttg tcctattcta
    25741 gtttcaaaac gctatagaag cagcattgta ctagtctaga ttcagtttac tatactaaaa
    25801 cgagtagctt gaaatcaaaa aacccaccct cacaggcagg ttttatctgt attattcagc
    25861 tagattatgc tttaccttct gaaccgaata cgtcgatacg ttcttcaacc gatgcttgga
    25921 tagcttttac accgtcagcc aagaatttac gtgggtcgaa gagttttttc ttgtcgtatt
    25981 ctgcttcgtt tgcttcgtag tcacgagcaa atttacgagt tgcgttagcg aatgcgattt
    26041 ggcattctgt gttaacgtta actttggcaa caccaagttt gatagctgct tggatttgct
    26101 catcaggaat acctgatcca ccgtgcaata cgattgggaa tcctggaaga gcttctgtca
    26161 atttttgcaa gtggtcaagg tcaagacctt cccagtttac tgggtaaggg ccgtggatgt
    26221 taccgatacc agctgccaag aagtcgatac cagtttcaac cattgcttta gcgtcttcga
    26281 ttggagccaa ttcaccttta ccgatgattc cgtcttcttc accaccgata gtaccaactt
    26341 cagcttctac tgagatacct ttagcgtgtg ctttttcaac aacttcttta gccaatttaa
    26401 ggttttcttc aactggaagg tgtgaaccgt caaacatgat tgaagtataa ccaacttcga
    26461 tacactcaag tgcatcttcg tagtgaccgt ggtcaaggtg gatagctact ggtacagtga
    26521 tacccattga ttcaacaagg ttagcgatca agttgcgagc aactttgtaa ccacccatgt
    26581 atttagcagc acccattgaa gtttggatca aaactggagc ttttttagct tctgctgcgc
    26641 gcaagatagc ttgagtccac tcaaggttgt ttgtgttaaa tccaccaact gcataaccgt
    26701 tgtcacgggc tgcttggaca aatttttctg ctgaaacgat tgccatttta tcaggcctcc
    26761 tgtatatttt tatgggtcat cccatttaca ttgttcattt tatcactttt tgccaaaaaa
    26821 atctagtttt tcccgcagtt tcgattgatt ttcttctaac tccatctatg taaacccttt
    26881 ctctccctag tcttgggcga cttttggaaa agctataaag aaggttaaac gattctcctc
    26941 catctcgaaa cgataagcta atttttcatg ttctaataga ctcttaacca caaagagccc
    27001 cataccagac cccttgacct tgcgactggc attgtcagaa aaagactggg ctagtttttc
    27061 ttgttcctct gagctacagc tattttcgat aaaaagttct ccttctcttt ctccaattcg
    27121 aactaagcca cctggaacag agtgcttaat ggcattgctg atgagattag aaagaatcaa
    27181 cttcataact gatgggttta gataagcctg ctgatgggtc aaactattgt ctatctggag
    27241 ctctctttcc ttggctagca aggcataatc tttgaccaga ttttgcgtca tctggaggag
    27301 gtcaattgtt tccctatcat ctcgcaattc ctgcacagaa gagagggaaa gtatctgcag
    27361 aacatggtga ttgagttcat ccacaatccc caaggcaact cccagatact ggtctctatc
    27421 cttataacga ccgatattct ctctcatatt ttcgattagg attttcaaac tagccagcgg
    27481 tgttttcaat tcatgagaag ctcctcgtag gaattcgacc ttcatcttct ccagctggag
    27541 aatggcttca ttcttttcat gcaagtccgc aataacagtc aagagatgct ggtagaggct
    27601 attgatttgt tccttgagat tacctatctc atccttagaa tccacgcgca atcgcacttg
    27661 ggaatccagg tccatcatcc gacgggtcac ccgcttgatt tccaaaatcg gtgcaacaat
    27721 agtccgggcg tagatgtagg ccaccaagag ggaaatcaga aaggaggcca gcaaggtata
    27781 gggaagaaac tggagactga tttgctccgc ttccttttgt aaatccatgg aagctagaaa
    27841 ctggagaatc atagtaccac cgtcttgcgt tttcacctcg cgctcctcaa taaagagaga
    27901 ggttgtctgg cggtctgtgt ccagaggaag actgtccttg acttctaact tgtcctcggt
    27961 catctcacct ttgacggtcc ccttgatatc actagtctgg gaatacaagt ctaacacttg
    28021 ctcgatactc tgcctatctt tcccttctag ggactgggca atggctgttg ccttttgacc
    28081 aatggtttcc tgacgatgac tcagataagt cgaaggaaaa agaaaataaa tagctaaatg
    28141 aaggcagata accagaacac taaatatcga gaaggtatag ataaatatct ttgcaaataa
    28201 acctgttcgt ttcattttcg ctccaattta taaccaacat tgcgcacagt gaggatacaa
    28261 tccaagtcta gctttttccg caattccttg atataaacat caataacacg gtcaaaggga
    28321 acctcatctg tcgctttcca gacggcatcg ataatctgag accgagtcaa ggcccggcct
    28381 tcatttttca ctagatagtc cagaatttcc aactctttgg cattgatagg cacttcttga
    28441 cctgcgaggc ttgcactgta gctttcaaag tccaccttgg tatccttgta agaaaagatt
    28501 cgtcctgtat cgtagtagcg cttgaaaatc gcgtccaccc tcacttttaa gagggagaga
    28561 gagaaaggtt tttccagata gccatctgcc aaagaggcaa aggcactcat cttgtattcc
    28621 tcatcctgaa aagctgtcaa catcaagaca ggaacctgac tggttttacg aatctcagct
    28681 aggacttcta agccgttaag cttgggcatc tggatatcca gtaaaaccag ggccacctca
    28741 tagctagaaa attgctccag agcttcctga ccgtccgctg cctcaatagt ttcatagcca
    28801 caatccgtca aataatcact gaccccctca cggatcatct cttcatcttc tacaattaaa
    28861 attttcatac tttaactgct ctctattttt tatttttctt agaataaata cctactctat
    28921 tttctattat agtctcttgc tggccttttg tctgtaagca actgatcact agataaaacg
    28981 ttgtgaaatt cctttctcat aaattccata actttagtat attatattta agcactaaag
    29041 tacaaagaaa gcaactgaaa gcaatgattt tcaccactgc tttcagattt attttgaatt
    29101 gttaaatagc tattcctatc cactattctt gaatagaaac acaagatgca atctttattc
    29161 cagactcatt ttttcaaatt tattcaccat ccagcaagag ctcttttggt tgttttctaa
    29221 ggagattgct tgaagcaagc gccataacga gaaccactag aaccaaggca aggacaaaaa
    29281 tgatgataaa gtctgatgtc tgaatggaaa tgtctaggct cgacaaggtc ttgctaaagc
    29341 catctacttc tgcaccgcca ccaaggttag aggcttgagc cgccttacta gcctgtttgg
    29401 caacacctga agtcacattg gcaaggacag tgtttccaat tgcacgggca gtgtaattag
    29461 ctaggaagta agcagaaact agagcaggga tagcaatcaa gatagattcg gtgatgaatt
    29521 gacccaagat acttgcctgc ttgaggccga tagagaggag aattcccact tccttgcgac
    29581 gggcgttgat ccaaaggctg agcaagaggg caaggaggag aactgagaag ctcaagctac
    29641 cccagaagag gaggttggcc atcttgtaca taccagagat agattgctca agagctgggt
    29701 agttagagga gctcttgacg agtgtgtagc tcttccagtt gataccactg atgccattca
    29761 actctttcat aacatcatcc aagttcttgt ctgctgttac aaagaaggtt gcgtccccat
    29821 aaatggctgt gtcttctgtg tatccataaa gttttgcagc agtgtgaatg tctgtaatag
    29881 ctgtgttttc gtaaagttct tgtgagtagg ttactgctga cttattatga ccatcaaaga
    29941 gtcccttgat tgtcacttca actgtttcct tggctccttt ttcattatct gcatcgtaga
    30001 tattagagtc cagtttaacc ttgtccccta ctttccagcc gtgtttggct gccaagtcct
    30061 tgtgcaagag gattttatcc ttgtcgtcgt tggttaagtg ctctccttcg actagtttat
    30121 aagaaccaga gacaaacttg tcttctttag aggagtcatt gacacctgta atcatcaagc
    30181 tacttccaaa acgtttggca cgatcagcag tgagattctt cttggtttct ggcgtttcaa
    30241 tcaggtcata tccagtcaaa tctccgatag cgttgatacg tttgacataa gactcaatgg
    30301 ccttgttttc ggtgattttt ttgatgtctt cacccttgat attcccagca ccgcgaggcg
    30361 ttccttggtt gacgcgacga ttgatttgca tggagaagct attggtgata tttttaaagg
    30421 tctcctgaga agccttggca gtagctccct tgattgacaa gccgaccaaa ctcaagctcg
    30481 ccatgaggag aataatcagg aagatgacaa tcgatttgaa aaacttcctt gtaacatagg
    30541 caaatgcgtt gtgtaacata gattcccttt ctagattttg ttttaatcat tctattaaag
    30601 taagctcaaa ttatttacta gtattgcgcg tttcagtcag tttcttatcc tttaattcaa
    30661 gtgtaatatc tgacgcttgt gccacttctt tactgtgagt tacgacaatc acacatttac
    30721 ctgttttctg ggcaagtgat ttgagtagtt cgacaatatc tccagcagtt ttaggatcca
    30781 gatttcctgt tggctcatca gctagaataa ctggagcttc tgagaccaaa ctgcgagcaa
    30841 tggcaacacg ttgctgttga ccacctgata actggagaac attccgcttg atctggcttt
    30901 catccaaacc aagctcaaga agtgtattct tgcttgcctt tttgttgacc aatcggatat
    30961 tttccagcgg agaaagataa tctatcaagt tataattttg aaagaccagg gaaatatggt
    31021 gcatgcgatg gtaagaatag cccttcttac gaatatcctc tccttgaaaa aggatagaac
    31081 cttcaacagg actatctaga ccagcaagta gggacaagag tgtggatttt cctgctcctg
    31141 actccccaat aatactgtaa aattttccgg gttcaaaatt ataattgatc tgatatagga
    31201 ctgcttcagc agtattctta taacggtagg taacatcttg taattgtaat aaagtcatga
    31261 tttctccttc ttaactaata gatgataaaa tttctttcgg tgattttcta aataagaata
    31321 ggaaacaaag ggctacagat aagcaactaa gcagaactag aaaaacatag gattctgcaa
    31381 aagataagat gctagttgat aaactgcttg ctttggctag tgtatcttgt aagcttgcct
    31441 gatctccact tgctagtaga gtttggagta ggtaagttgt gattccgttt cctgcaacaa
    31501 atgctggaag caaagctcca agagatacca aaactacctc taaacagaat tgtaggaaaa
    31561 tcgagctctt gccttttcca agtgcaagta aaatccccac ttcatagacc cgttctctca
    31621 accagagaga caaaaccaga attaaggctc cagctcctgc tatcaacatc ccataaagga
    31681 agatggtcag gaaggtttgg aaagttgcaa ctgagtcttt gatttgttca aaagccttgt
    31741 tttccttttc gacttggtag ccttgatttt ccaaggccaa gttttctacc tgcttcatga
    31801 gtccgtccat ttccttagga ttttctacat agaagcgtgc tgcactgact tgagcttcac
    31861 tattgcccaa aagggtttgg ctactttcat agtctgtaaa gacttgattt tcactgaagt
    31921 cagaagacaa gcctgtgaat ttctcttgtt ttttaccaga aaagatgccg ataatctcaa
    31981 actctactgt ttgtcctttt ccagattcag actgaccagc atccaagcca atcttgtcat
    32041 gaagtgaaag accgttcttc ttagccaatt cttcgtggat aaggattttc ttggaattcc
    32101 ctttttgaag gtgtcgccct tcttttagat tgaaagccga actggtaaag gttacatcct
    32161 tggatgaatc ctcaagagcc gttaagctaa ccaagttatt gtctgcagct gataaatcat
    32221 cacgctccac gctctgctcg ccagtcactg cttccttgtc ttttagtttt gcgaccgtct
    32281 cgagttcagg agagacattt tccagcccct taatcttgct tacagatgct aggtctgaca
    32341 acttgaatgt ctgaccattc tctatcttct taatagaaaa agatgtattg agtgatttat
    32401 aaagattgct ttctactgtt ttgttggact tcatcagagt caaacaggct gaaattccgg
    32461 ccaataagac caataaaatc agaaataaaa taaaacttct cagtcgcttt ctgctgacat
    32521 aagcccaaga tctttggatt ggattcattt gtcacctcca tatttgtaag actattataa
    32581 aacccaaata tgaaatgttt atgaaatacg aaaaaaaaat atcgagtagg ggataatctc
    32641 tagcccctct ca
//
LOCUS       DAVKFH010000023        31431 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKFH010000023 DAVKFH010000000
VERSION     DAVKFH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.023"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(694..1266)
                     /locus_tag="VW006_001106"
     CDS             complement(694..1266)
                     /locus_tag="VW006_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379971.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(1888..2736)
                     /locus_tag="VW006_001107"
     CDS             complement(1888..2736)
                     /locus_tag="VW006_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9379972.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            2888..3166
                     /locus_tag="VW006_001108"
     CDS             2888..3166
                     /locus_tag="VW006_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9379973.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            3168..5231
                     /locus_tag="VW006_001109"
     CDS             3168..5231
                     /locus_tag="VW006_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9379974.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLVRTKELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(5309..6565)
                     /locus_tag="VW006_001110"
     CDS             complement(5309..6565)
                     /locus_tag="VW006_001110"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9379975.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            6707..9172
                     /locus_tag="VW006_001111"
     CDS             6707..9172
                     /locus_tag="VW006_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9379976.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            9237..10148
                     /locus_tag="VW006_001112"
     CDS             9237..10148
                     /locus_tag="VW006_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9379977.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            10378..11076
                     /gene="dapD"
                     /locus_tag="VW006_001113"
     CDS             10378..11076
                     /gene="dapD"
                     /locus_tag="VW006_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9379978.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            11144..12274
                     /locus_tag="VW006_001114"
     CDS             11144..12274
                     /locus_tag="VW006_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9379979.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            12286..12825
                     /locus_tag="VW006_001115"
     CDS             12286..12825
                     /locus_tag="VW006_001115"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9379980.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            12809..13486
                     /locus_tag="VW006_001116"
     CDS             12809..13486
                     /locus_tag="VW006_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9379981.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(13558..14457)
                     /gene="galU"
                     /locus_tag="VW006_001117"
     CDS             complement(13558..14457)
                     /gene="galU"
                     /locus_tag="VW006_001117"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9379982.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(14479..15495)
                     /locus_tag="VW006_001118"
     CDS             complement(14479..15495)
                     /locus_tag="VW006_001118"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9379983.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            15707..16147
                     /locus_tag="VW006_001119"
     CDS             15707..16147
                     /locus_tag="VW006_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9379984.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(16270..16686)
                     /locus_tag="VW006_001120"
     CDS             complement(16270..16686)
                     /locus_tag="VW006_001120"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9379985.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(16754..17404)
                     /gene="phoU"
                     /locus_tag="VW006_001121"
     CDS             complement(16754..17404)
                     /gene="phoU"
                     /locus_tag="VW006_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9379986.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(17419..18171)
                     /gene="pstB"
                     /locus_tag="VW006_001122"
     CDS             complement(17419..18171)
                     /gene="pstB"
                     /locus_tag="VW006_001122"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9379987.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(18173..18988)
                     /gene="pstA"
                     /locus_tag="VW006_001123"
     CDS             complement(18173..18988)
                     /gene="pstA"
                     /locus_tag="VW006_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9379988.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(18981..19844)
                     /gene="pstC"
                     /locus_tag="VW006_001124"
     CDS             complement(18981..19844)
                     /gene="pstC"
                     /locus_tag="VW006_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9379989.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(19962..20837)
                     /locus_tag="VW006_001125"
     CDS             complement(19962..20837)
                     /locus_tag="VW006_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9379990.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(21004..22335)
                     /locus_tag="VW006_001126"
     CDS             complement(21004..22335)
                     /locus_tag="VW006_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9379991.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(22336..23043)
                     /locus_tag="VW006_001127"
     CDS             complement(22336..23043)
                     /locus_tag="VW006_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9379992.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(23127..23603)
                     /locus_tag="VW006_001128"
     CDS             complement(23127..23603)
                     /locus_tag="VW006_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9379993.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            23610..24137
                     /locus_tag="VW006_001129"
                     /pseudo
     CDS             23610..24137
                     /locus_tag="VW006_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24452..26143)
                     /locus_tag="VW006_001130"
     CDS             complement(24452..26143)
                     /locus_tag="VW006_001130"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9379994.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            26279..26725
                     /gene="argR"
                     /locus_tag="VW006_001131"
     CDS             26279..26725
                     /gene="argR"
                     /locus_tag="VW006_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9379995.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            26776..29310
                     /gene="mutS"
                     /locus_tag="VW006_001132"
     CDS             26776..29310
                     /gene="mutS"
                     /locus_tag="VW006_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9379996.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            29457..31151
                     /gene="patA"
                     /locus_tag="VW006_001133"
     CDS             29457..31151
                     /gene="patA"
                     /locus_tag="VW006_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9379997.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         9030 a         5974 c         6851 g         9576 t
ORIGIN      
        1 tgtttttatg atatacacac ttaaattggt gttgtttatt acctttcttg taataagctt
       61 gttacctgat aagatttttg gaaaaaataa aaaaatttgg aaaatagttt ttgcaatatt
      121 gacggcagtg gcagcattgt catttatgta ctaagttatt ttaagaatgt agggaaataa
      181 accctacatt tttttagttt tttctgtttt ctaaattcta tttatccaag agcttcaaca
      241 tttcttgctt cttcgcttca agttctgcac gcttttcttc gatttcggca tgttttttct
      301 cgagttcaga acaacttgca tcattgctaa attcttttcg ccatcaggag atagggtgag
      361 tctacatgtc tattactcac ccaaagcagt cctacaaagc aggaattttc tgttactttt
      421 ttggaaatag taacgtttat acagctttga cacttcgtat caaagcgcca aacacactcc
      481 gagggattta cagaaagcag aaaaggaatg atctggtata agatcattcc ttttctctct
      541 ttttctttaa gtaattatat acaatgtacg acgaagtcgt cattgcaatg ctgatccacc
      601 acctaaaggg aactttaaac aacattgata agataaagaa tataaacaac gaaaatacgt
      661 tatacccaat taattttatt gtatatctca tgattaaaag ttaatccttc cgttgttagg
      721 aatggcatca tttttatccc ataattgtgc taaataagtc cccggtgata ataaattcat
      781 agcgaattct aaagcaacat catttacaaa ccaactacct agatatctag aaattgctga
      841 acgaatagca ctttttgctg catgttttcc ttttacttta attagatttg caaggcctgc
      901 agtagttcct cctaatgcta aagctattgc agtatctaat agagcaccca tttgattaac
      961 tgtaatacct tgccaagctg ctctaaatgg agagtatgta ggtgggattg tataatcgcc
     1021 ttgtaattgt cggttaatta cttctttgat ccattgttgt gagacgtctg gatgaaaaga
     1081 ttggatttcg tttgcaagtg tattgatttg ttcttctgtt agagaagtga caggttgaag
     1141 ttccatattt gtttcaattt gtgatacttg ttcagaagcg tatacagctg aaacacttgg
     1201 aatcgctgat acaattaaca caattgacgt caaaaaaacc gaaataaatt tcattaattt
     1261 gttcatgagc ttttctcctt tttatttgta tctgcttaca ttttatcata tactgttatt
     1321 atagtcaaaa aaatatgcta ttatgttaaa aaaatatttt tcaaaatata aatggacgga
     1381 tttattttgg attttatttg ttattttgac ctgcctctat attggtaacc atgatttgtt
     1441 tactctcaat catcaagaat tctcttttcg tggtagcgtt tggggtctgg tactggcctt
     1501 atatcactta ttgttcattg ataagtttgt aatttcgaat cgaaaataaa gattagaact
     1561 atgattgact ggctactttt aatattgatt ttggaggaag attttgtctc tattatttat
     1621 tattttaaat ttatctattt tgtacaagat ttattctttg agacgggaga aatcgaaata
     1681 cttgatttat acggcctata tcatatttgg ggtaaatgta gtatatggta ttcaatggtt
     1741 attaagagaa ctgatttcaa ctatttcccc ttaatgtgta tggaaactat aacaaaacgc
     1801 ataagattaa ggtttgtgta cctgttctta tgcgttttta taatattgaa gattttcggt
     1861 aagttttata cctttgggaa gtctagttta gaatgctttc ccaaccaaaa tctctgcttc
     1921 gatggtaatc tctgtcagtt ggctccagtc aatcttgctc tggtcaacgt gaaagaggag
     1981 aggagtcata ctgagtaggg cttggagttg ttctgctgtg atagtcttag tcagagaggc
     2041 agtttgacta gataggatgg taaagtgttc ctggaaatgc tccttgatat cttggttaga
     2101 atactccttg tttgtcagct ggtcctgtac tctttgacgg atttctttga ggtgattttc
     2161 agttgggata acctttatca agataccgtc tttggataaa acgcgacgaa attctccata
     2221 gttggcaggt gagaagatat caagcagaat atccatgtta gcgtctttta taggaagtcg
     2281 tgccaagtcg ccaacaaacc aattgactgc ccagttgggt tcacttttag cagcgatttg
     2341 gactgaatct ttggagatgt caaaggcata gaaagttttt tcagagtgac tttcttgtag
     2401 tttgcgagaa tagaatcctt caccacaacc gatatccaaa attgttgtgg tagtttttga
     2461 gcttgcaagc aagtcagata cagcatctaa gatagcttgg taaaagccgg cttctaggat
     2521 ttgttgacgg ttttgaaaat tttccttgtt atagttagca gattgcttga tttgaggtgc
     2581 tagattgaca tagccaaatt tcgccaagtc aaaagaatga cggttgcagc acttgaaatt
     2641 agtctctaac agagtcagat tttcttgaca gataggacag gcaaaggcag tcgcagaagc
     2701 aaaacgctga agtttgggct tgagatttgt attcatagtt taagtgtatc aaaagaggca
     2761 aatgaaagca actttaggaa taagtagatg ggagattcag tagaaataaa actaattctc
     2821 caatttatag aatgaaattg cttttatatc taaattgcta tataataatg ataaggagga
     2881 aataagtatg ttaaaagaag tattaaccgt tgcaaaagtt gcgaaaaaat cttcattatt
     2941 tttgggtggt gtcgcatttg gtacccttgg tttgaaaatc ttagcaagta aggaagctaa
     3001 aaaaggttat tctaaagctt tggctaaggc ttacaagttg aaagacgagc tagatgcatc
     3061 tgtttctgtt gtgaagcaac atggagacga tgtcttgcaa gatgccaaat atttgtacga
     3121 gcaagagaaa aaagaagagc aattagatag ccttataggt gaataatatg tcttttaaag
     3181 tgctacatag aggataccaa catatccgac tatcatcttc tttttcactt accttggata
     3241 ttcaagacta tcttcgttcc ttggcgagag atgaaaaggg gattgagtct atccagtttt
     3301 acatggatca acagcacttt actctacgca taaaagaagg cttttctgta ttagataatg
     3361 cagaagcctt tttaaaaaga attgataaag ggaaagtttc tgagttgatg actcttccca
     3421 ttcgtagaga agagagtgct tattctattg tttcaggtgc agcggttaag cgtgtacttt
     3481 ttcgtagttt tgtgccgtat cctattcgct atatatggac ttgttatcag gctttgggtt
     3541 atattagaga agcctatcaa acactagcgc gtaaggaact aacgatggaa gtcttggact
     3601 gttcggctat tttattgtct ttgtttatga accaatccaa gacagctagc aatatcatgt
     3661 ttatgcttga tttggggaat catttagatc agtggtcctt gaaaaaaact gcaacagatt
     3721 tagaacagag tcttcttgca aaagagagcg atgtattcct agtacagggc gatacggttg
     3781 ttagtatcaa gagttccgat gttcaaatag gagatgtctt gatcttatct caaggaaatg
     3841 aaattctgtt tgatggacaa gtagtttcag gtttaggtat ggtcaacgaa agttccttga
     3901 caggagagag ttttccagtt gaaaaaagag agtctgattt ggtttgtgca aatacagtat
     3961 tagaaactgg agagttacgc attcgtgtaa cagataatca gatgaacagc cgtattttac
     4021 agctgattga gttgatgaag aaatctgaag aaaacaagaa aacgaaacaa cgctatttca
     4081 tcaagatggc ggataaagtc gtcaaatata atttcttggg ggctgggctg acttacctat
     4141 tgacaggttc tttttctaag gctatttctt tcctattggt cgatttctcc tgcgctttga
     4201 aaatctctac tcctgtagct tatttgacag ttatcaagga agggttgaac cgtgaaatgg
     4261 tgattaagga tggagatgtt ctggagaaat atctggaagt tgatactttc ttgtttgata
     4321 agacaggaac aatcacaact agttatccta tagttgaaaa ggtgttacct tttggagact
     4381 atagtgagga agatattctc agaatcagtg cctgtcttga ggaacacatt tatcatccta
     4441 ttgctaatgc catcgtcaag caagctgaga tagagggaat tgaacatgag gaaatgcatg
     4501 ggaaactcca atatatcgca agcaagggga tcaaatctca tatagatggc caaccagttc
     4561 ttattgggaa ttatgtcttg atgcaggatg agcagattca tatcagttca gaacaaaatg
     4621 ctttaattga agagtacaag agtcactaca atctcttatt cttggcttat caaaatgaat
     4681 tgattggaat gttctgcatt catactcctt tgagaaaaga agcaaaaaca gccttggata
     4741 aacttaaggc acaggggaaa aaattgattc tggcaacagg ggacaccttg gttaggacaa
     4801 aggaattagt caaagatttg ccctttgatc aggtctatac agacttgaaa cctgatggga
     4861 aatttgagtt agtagagaaa ctgcagaaag caggtcacac tattttgatg gttggagatg
     4921 gattgaatga ctcagcggct ttaactctat cagatatcgg tgtggtgatg aatgagagtg
     4981 cagatatttc taagcagatg agtgatatct tattgttaga taatcgtttg gatttcttcc
     5041 aagagttgga ttcgttatca tcatctttgc aaacactcat caagaaaaat attcaagata
     5101 ccgttgtcgt aaatagtagt ttgattggct tcggcttatt taattggctc agtccttcaa
     5161 atctctctat cttacataat ctaacaacct tgcgcattgt cctgcgtagc ctgtctatta
     5221 aaaatagata gatgagagtt attaacagca aaaaggagtt acctttctcg aggttaactc
     5281 ctttgtttat agataaatgt tgaatagttt agtaagtcaa tacaaagtat tttttcttcc
     5341 cacgacggat aacagtcagt tcattctcta acttatcagc gtcactcaag acatagtcaa
     5401 gctcttggat gcggtcgccg tttacgtaga tggctccgtt ttggacgtct tcacgggctt
     5461 ggcgttttga gttaactata ccagatgaga cgagcagttc cacgatattg ttgttttcgt
     5521 ctgcctgtac ttggtagttt ggcacaccac gaagtccttg tttgagctct ttgacagaaa
     5581 ggtttttgat gtttcctgca aagagttgct cagtgatgtt aagtgcttct ttgtaggctt
     5641 cttctccgtg aacaagtgta acaacttcac gagccaagac tttttgagcc aagcgttcgt
     5701 gtggcgctgc ttcaaattgt ttacgaatat cttcaatctc atcaagtgac aagaaagtaa
     5761 agattttcaa gaagcgaaca gcgtcagcgt ccatcacgtt catccagaat tggtacattt
     5821 cgtatggaga agtcttttcg ggattgagcc agacggcatt tccttctgat ttaccaaatt
     5881 tcttaccagt tgcatctgtg attagtggaa cagtgataac gtgaccagtc ttgtccgcct
     5941 tacgacgaag caattcggta ccagctgtca tatttcccca ctggtcagaa ccaccgattt
     6001 gaagagtgac attatggtct tggttaagga cgaagaagtc atacccttgc atgatttggt
     6061 aagcgaactc agtgtaagaa attcctgttt cgatccgttt tttaacagat tccttactca
     6121 tcatgtagtt gaccgtgaag tattttccaa tatcacggag gaagtcaatg aagctgatgc
     6181 tgccaaacca gtcgtagttg ttgaccatga cagccttgtt ttcgccattt tcaaagtcaa
     6241 gaaaacgaga aagttgtcct tggatagact tgacccagcc atctactgtg tcttttgttt
     6301 ggagactacg ttcagcatct ttgaaggacg gatctccgat gagacctgta gcaccgccaa
     6361 cgagcgcata aggtttgtga cctgctagtt gcaagcgacg acttgtcaag attgcgacaa
     6421 ggtggcctag gtgaaggctg tcagcagttg gatcgtagcc agtataataa gaaacttgac
     6481 cttcttctag ggctttacgc aaagcttctt catcagtcgt ttgaaatatc aaaccacgct
     6541 cttttagctc atcaaaaatg tgcatgtgtt ttttctcctt tttaaaatta ttgtttctac
     6601 ctattgtatc acaaacttgg gcaatagcct agcagaatag ggaagaaagt ggctatttta
     6661 ttgaaaattt cccttttgtg gtataataga taaagtgagg aaatccatgc aaaatcaatt
     6721 aaatgaatta aaacgaaaaa tgctggaatt tttccagcaa aaacaaaaaa ataaaaaatc
     6781 agctagacct ggcaagaaag gttcaagtac caaaaaatct aaaaccttag ataagtcagc
     6841 cattttccca gctattttac tgagtataaa agccttattt aacttactct ttgtactcgg
     6901 ttttctagga ggaatgttgg gagctgggat tgctttgggg tacggagtgg ccttatttga
     6961 caaggttcgg gtgcctcaga cagaagaatt ggtgaatcag gtcaaggaca tctcttctat
     7021 ttcagagatt acctattcgg acgggacggt gattgcttcc atagagagtg atttgttgcg
     7081 cacttctatc tcatctgagc aaatttcgga aaatctgaag aaggctatca ttgcgacaga
     7141 agatgaacac tttaaagaac ataagggtgt agtacccaag gcggtgattc gtgcgacctt
     7201 ggggaaattt gtaggtttgg gttcctctag tgggggttca accttgaccc agcaactaat
     7261 taaacagcag gtggttgggg atgcgccgac cttggctcgt aaggcggcag agattgtgga
     7321 tgctcttgcc ttggaacgcg ccatgaataa agatgagatt ttaacgacct atctcaatgt
     7381 ggctcccttt ggccgaaata ataagggaca gaatattgca ggggctcggc aagcagctga
     7441 gggaattttc ggtgtagatg ccagtcagtt gactgttcct caagcagcat ttttagcagg
     7501 acttccacag agtcccatta cttactctcc ttatgaaaat actggggagt tgaagagtga
     7561 tgaagaccta gaaattggct taagacgggc taaggcagtt ctttacagta tgtatcgtac
     7621 aggtgcatta agcaaagacg agtattctca gtacaaggat tatgacctta aacaggactt
     7681 tttaccatcg ggcacggtta caggaatttc acgagactat ttatacttta caactttggc
     7741 agaagctcaa gaacgtatgt atgactatct agctcagaga gacaatgtct ccgctaagga
     7801 gttgaaaaat gaggcaactc agaagtttta tcgagatttg gcagccaagg aaattgaaaa
     7861 tggtggttat aagattacta ctaccataga tcagaaaatt cattctgcca tgcaaagtgc
     7921 ggttgctgat tatggctatc ttttagacga tggaacaggt cgtgtagaag tagggaatgt
     7981 cttgatggac aaccaaacag gtgctattct aggctttgta ggtggtcgta attatcaaga
     8041 aaatcaaaat aatcatgcct ttgataccaa acgttcgcca gcttctacta ccaagccctt
     8101 gctggcctac ggtattgcta ttgaccaggg cttgatggga agtgaaacga ttctatctaa
     8161 ctatccaaca aactttgcta atggcaatcc gattatgtat gctaatagca agggaacagg
     8221 aatgatgacc ttgggagaag ctctgaacta ttcatggaat atccctgctt actggaccta
     8281 tcgtatgctc cgtgaaaagg gtgttgatgt caagggttat atggaaaaga tgggttacga
     8341 gattcctgag tacggtattg agagcttgcc aatgggtggt ggtattgaag tcacagttgc
     8401 ccagcatacc aatggctatc agaccttagc taataatgga gtttatcatc agaagcatgt
     8461 gatttcaaag attgaagcag cagatggtag agtggtgtat gagtatcagg ataaaccggt
     8521 tcaagtctat tcaaaagcta ctgcgacgat tatgcaggga ttgctacgag aagttctatc
     8581 ctctcgtgtg acaacaacct tcaagtctaa cctgacttct ttaaatccta ctctggctaa
     8641 tgcagattgg attgggaaga ctggtacaac caaccaagac gaaaatatgt ggctcatgct
     8701 ttcgacacct agattaaccc taggtggctg gattgggcat gatgataatc attcattgtc
     8761 acgtagagca ggttattcta ataactctaa ttacatggct catctggtaa atgcgattca
     8821 gcaagcttcc ccaagcattt gggggaacga gcgctttgct ttagatccta gtgtagtgaa
     8881 atcggaagtc ttgaaatcaa caggtcaaaa accagggaag gtttctgttg aaggaaaaga
     8941 ggtagaagtc acaggttcga ctgttaccag ctattgggct aataagtcag gagcgccagc
     9001 gacaagttat cgctttgcta ttggcggaag tgatgcggat tatcagaatg cttggtctag
     9061 tattgtgggg agtctaccaa ctccatccag ctccagcagt tcaagtagta gttctagcga
     9121 tagcagtaac tcaagtacta cacgaccttc ttcttcaagg gcgagacgat aattctctaa
     9181 atgaagtggc caatcaatgg attgccactt ttttcttttc gtgttacaat aagggtatga
     9241 atcagtatca gaaaaagatt gttaacggaa aaatttattc gctcctatcc ggcttaatat
     9301 ggggaatctg tggaatttta ggagagtact tctttactca ttatcaggtg tcttcgggct
     9361 ggattacctc tatgcgtttg acactggcag ggagtcttgt actcatttgg tctgcaatac
     9421 aattaaaatc gcaagtgcta gatatttggc gagacaagaa aaattacctg ccctttttag
     9481 cctatgctat tttggggatt ttttcagttc agtatttttt ctatctctgt gtagaatact
     9541 caaatgctac gacagcaact attttacagt ttattagccc tgtctttatc ctcttttaca
     9601 atcgcttggt ttatcaaaaa cgagcgtcaa aaagcgctgt tttctatgtt ttggttgcca
     9661 tgctgggtgt ttgcttgatg gcgacaaagg gagacctctc tcagttatcc atgacgccgc
     9721 tagctcttat aacaggtttg ctgagtgcca tgggtgttat gtttaatgtt atcttgcccc
     9781 aaccttttgc taagcgttat ggttttgttc ctacggttgg gtgggggatg attttggcag
     9841 gtttgtttag caatgtcctc tcgccggttt atcagctttc ctttactctt gatatttgga
     9901 gtatcttgat ttgcctcatt atcgctttct ttggaacggc ttttgctttt ttcatttcca
     9961 tgaaggctgt gtccttggtt tctcctttgg tggttgccgt tatcagtgcc agtgaacctc
    10021 tctcttctgc tctcttgagt gttttgttct taggattagt agtggattgg tccctccttc
    10081 tagctatagc cttgattatt ttacccatga tatttttgtc tatagaagaa gcgaaagaaa
    10141 gtagataaaa agtcttttct taattctaaa agtgtgctat actagaatag tcaataaaac
    10201 acggggagat agttgtgaag agtgttttta ggttgtatcg gtaggggctt gcttttaccg
    10261 acagcacgtt ttatctcaca tccctaaaaa atcataccta aaaacagaca aaaaggtttc
    10321 aaatttgagg ccttttttgg tagaataggt atcattataa cgaaccagga ggcacctatg
    10381 actgctacaa aaatgaacgc tcaagaaatt atccaattta tcgccaatgc tgaaaagaaa
    10441 accagtgtca aagtaacctt tgaggggcaa ctcgcaactg ctgtgcctag ctctgttgtc
    10501 aaactaggaa atgtcctatt cggagactgg aaggacgtgg ctccgcttct tgaaggtttg
    10561 gtagaaaatc aagattatgt tgtcgagcaa gatgctcgta attctgcagt tcctttgcta
    10621 gataagcgtg ctatcaacgc tcgtatcgag ccaggtgcta ttatccgtga ccaggtggaa
    10681 attggtgaca atgctgttat catgatggga gctgttatca atatcggtgc tgaaatcggt
    10741 gctggaacca tgattgacat gggtgccatc cttggtggcc gtgccatcgt tggaaaaaat
    10801 agccacgttg gtgcaggtgc agttttggca ggtgtgattg agccagctag tgctgaacca
    10861 gtccgtgtcg gagacaatgt tctcatcggg gctaatgcag tggttatcga aggagtccaa
    10921 atcggtagtg gttcagttgt cgcagcagga gctattgtta cccaagatgt cccagaaaac
    10981 gtggtagtag caggtgttcc agctcgtatt atcaaagaaa ttgatgccca aactcaacaa
    11041 aaaacagcgc tagaggatgc gcttcgtacc ttgtaattgt aaaagtaaaa aagaggcgga
    11101 accctttttc cagcctcttt ctgctatatc ggaggacaga tagatgttag atttgattca
    11161 gactagacga gatttacacc agattccaga gattggcttg gaggagttca agactcaggc
    11221 ttatttgctg gatgtgattg agaaattgac tacgggcaag gattttgttc aaattcgtac
    11281 ttggcggaca gggattttgg tctacctgca gggaagtcag ccggagcgaa ccattggttg
    11341 gcggacagat attgatggcc tgcctatcgt cgaacaaaca ggtctacctt ttgcttctca
    11401 acaccaaggt cgcatgcatg cctgtggcca tgattttcat atgaccattg ccttaggctg
    11461 tcttgagcgc gcccttgagg agcaacccaa gaataatctg ctctttctat ttcagcctgc
    11521 tgaagaaaat gaagctggtg gtatgctcat gtatgaggac gatgcttttg gagattggtt
    11581 gccagatcaa ttttatggcc tccatgtccg tccagatttg aaagtcggcc agattgcgac
    11641 taatactcat acactctttg caggaacttg cgaggtgaag atccgtttca aaggcaaagg
    11701 tggccacgca gcttttcctc atgaagccaa tgacgccttg gtggcggcta gttactttgt
    11761 gacccaagtg cagtcagttg ttagccgcaa tgtcaaccca atcgagggag cagtggtgac
    11821 cttcggcgtt tttcaagctg gaaccaccaa caatgtcatc acagacacag cttttttaca
    11881 tggaactatt cgtgccttga cacaggacat gagtctcttg gtgcaaaaga gggtaaaaac
    11941 agttgcagaa ggggttgcag ctgcctttga tatggaagtc gaagtggaac tcaaacaagg
    12001 tggataccta cctgttgaga acaatccagc cttggcgcgt gaactgatgg acttctttga
    12061 cgaaaaagac ggaatcgagt tgattgatat cgaacctgct atgacaggcg aggactttgg
    12121 ttatctcctt tcaaaggttg atggcgtgat gttttggcta ggtatcgata gtccctacgc
    12181 tcttcatcac cctcaaatga gtcctaagga agaagtctta gctattgggg tggctgcggt
    12241 gtctagtttc ctgaaaaaga aggcagcaga atagagggct tgtctatgaa atcggaatta
    12301 cgcaagcaag tcttgcatga aatgaaggct atatctcaag agcaaaaaca ggctatagac
    12361 caagctttaa ccgagcgaat tttacaacac cccttttatc aagaagccaa ggtcatcgca
    12421 acctacctct ctttttctca tgagtttcaa acgcgggaac tgattgagca ggcgctgaag
    12481 gacggcaaga aggttttaat acccaaaact tatcccaagg ggcgcatgga ctttgtagtc
    12541 tatgatccgc agcagttggt aaaaactgcc tttgccttac tggagccaca gggagatttg
    12601 gaagtggtgg atgcatctca gattgatttg attcatgttc ctggcctggc ttttacgacg
    12661 gatggatatc ggattggata tggtggaggt tattatgacc gctatctgga acatttttct
    12721 ggtcatactt tgagtacggt tcatccttgt caaattcagg actttatacc tgaaaaccat
    12781 gatattcctg ttcaggaggt attaattgat gaaggaaatc tttgatagac gttaccctgt
    12841 gacgagtttc ttcctcttag tgacggcctt ggtattttta ctaatgttgg tcactgcagg
    12901 cggaaacttt gacagggcag atacattatt tcgatttgga gccatgtatg ggccagctat
    12961 tcgcctcttt cccgagcagg tttggcgtct cttgtctgcc atttttgttc atattgggtg
    13021 ggaacatttc attgttaata tgctttcact ttattatctt ggaaggcagg tagaggagat
    13081 tttcggttct aagcagtttt tctttctcta tcttttatca ggaatgatgg gcaatctctt
    13141 tgtttttgta tttagtccta aatccttagc agcaggcgcc tctacctctc tttatgggct
    13201 atttgccgcg attattgttc ttcgctatgc aactcgcaat ccttatatcc aacagctagg
    13261 gcaatcctat ctgacacttt ttgtggttaa cattattgga agtgttctga ttccaggaat
    13321 cagcctagca ggccatatcg gtggtgcagt tggtggcgca tttctagcag ttatctttcc
    13381 agttagagga gaaaaacgga tgtataacac cagccagaga ttaggagcgg tagtcttgtt
    13441 cgtaggactc gccattttgc ttttctacaa gggaatggga atgtgacgaa gttgaaagaa
    13501 aatttgtgta atgaaaaaag ataaggcatt ttttgaacct tatcttttta ttttatttta
    13561 ttccttctca gtcaattctt ttccaagttg gatgaggtaa ttcttcaaat catctttgac
    13621 ttgtgggtgt ttgagggcgt agtcgatgga tgttttcatg aagccaaact tgtctccgac
    13681 atcgtaacga gccccattga actcacgagc aaatacacgt tgtgttttat tgagggtgtc
    13741 gattgcatct gtcagctgaa tttcatttcc tgcacctgga gcttggtttt cgaggatttg
    13801 gaaaatttca ggcgtgagga ggtagcgtcc gataatagca aggtcgctag gagcgtcctc
    13861 tggagctggt ttttcaacaa aggtttcaac actgtaaaga ccatcttttc cttcgccttg
    13921 cggagcaata accccgtaag cagatacttc gtcatgaggg actggcatga cagcgatagt
    13981 agacgcgtgg gtacgctcgt agtcatccat gagttgtttg gtaagtggaa cagccttttc
    14041 gtctgtgata tccatcaagt catcaccaag cataacgaca aaaggttcat ttccgacgaa
    14101 agccttggct tgcaaaacag catctccgag accgcgtgga tgagtttggc ggataaaatg
    14161 cagccgcatg tcagttgttt tatcaactag cttcaaaaga tctgttttcc ctttttcttt
    14221 gaggttatat tccaattcga agtttgaatc gaagtggtcc tcaatagaac gttttgattt
    14281 accagtgaca actagaatat cttcaatacc tgatttgaga gcctcttcca cgataaactg
    14341 gatagttggt ttgtctacga ttggcaacat ttctttggca agggccttgg ttgctggtaa
    14401 aaatcgagtt cctagtccag cagcagggat aactgccttt ctaacttttg atgtcataaa
    14461 aatcctttct atagagggtt aagaccactc attttctgct ttaaattcat tgttcatgat
    14521 gtcataaatg gcatctttga tattggttcc gtgataaata acttggtaaa tagcctgtgt
    14581 aatgggcata tagactccaa gttcttgggc tagttcatag gctgctcgag tcgttgaaat
    14641 tccttcgatt accatgccca tattagcttc tatatcagct agggattctc ctcgtccgag
    14701 agcatctcca gctctccagt tacgagagtg gatggaagtt cccgttacga tcaaatctcc
    14761 cacaccagat aagccgctat aggtcaatgg actggccccg agtgctaccc ctaggcgggt
    14821 gatttctgct aaacctcgag cgatgatggc tgccttagca ttatcaccaa atccaagacc
    14881 atgtaaagct ccagcaccga cagcaataat atttttaaga gcaccagcag tttcaacccc
    14941 gataacatcc gtattggtat aaagtcggaa gtagtgatta ctaaatagct cctgaacgta
    15001 ttgagctgtt tgtaaatctt tagaagcagc agttattaaa gttaggtcac gcacaatggt
    15061 ctcttctgca tgactaggcc ctgaaacaac gacgatatca ctacggagat gttcaggaat
    15121 ttcttcttca agaatggttg ataatcgttt atggctatca ggttctaatc cctttgatgc
    15181 gtgcatgatg ataaccttat ggtccaaggt ttgtgcaact tgctgggcaa caagtcgtgt
    15241 cacttttgtt gggacaacaa acaaaatcgc atccacattt ttcaatgttt ctgctaagtc
    15301 ggtgtaggca atgatatttt cgtctagaac gacatcttta aagtagtgct tattagtatg
    15361 gtgtgtatta atttcattga tttgctcggg aagatttccc caaatacgta cctcgtgtcc
    15421 attgtcattt aagacttgtg aaagggcggt tccccaagaa ccaggcccca agacggcgac
    15481 ggtttgtttt tccatgattt cctccttgat agatttcttt ctcttattct atcatattct
    15541 aggggatttt gcacttgcat tactggggta tggtggaaaa atacacaaag accgagatga
    15601 agggataaac tcttagtctt tagttaaagt gagaagaaat ctgatagata gtttgattca
    15661 gtatatgctt ataatagagg taaacaactc aggaagttct tgtaggttgc gagagtttgg
    15721 cgaaaaaatt aaaagattac gtttggctaa aaaaatcagt cgttcagaat tttgtggtga
    15781 tgagtctgaa ttaagtatcc gtcaattaat tagaattgaa aatggagaat ccagaccaac
    15841 actaacaaag ttaaaatata ttgctgaacg tttggaggtt gaagattaca agttgatgcc
    15901 aagttatata gagttggata aggaatacct agaattgaag tatttcttga tgaggactcc
    15961 tacatacgaa gatgaaacta tcgcccaaaa gaaagagagt gtttttgata agatttttga
    16021 agagtattat gataggctac ctgaggaaga aagatttatc atcccaaatt attcatatct
    16081 agcactagcg aactacacag ttcaaaaatt accagaaaag ctagttgaaa tactgtcctt
    16141 ctggtgattt tgcttaattt ctgatgttcg agacatgatt gaggagattt gtcttgattt
    16201 tttcctaaaa aagggcagac tcctcccttg gtttgatgct cgatttttaa aataaggtga
    16261 cttattctgt taggcagttt tcccctgccc tctagctcta tatggaacag ggagtatcag
    16321 atttatctgg cagattgtat caaataaggc ttgaaactgt tttgaatagg cgtatagact
    16381 tgagaatttg agaatatgtc gtctagcagt agagacatat tttccggcaa tatgaaggaa
    16441 gagacgtcga aaacgcttga tagtcaaggc ctttacttca tcaccagcta gctgctttaa
    16501 aaagaggtag agattataag ccagacagcc catcatcata cgtacttcat tcttaatcat
    16561 ggtcgaacta tctgtcttat ccccaaagaa tcctgctttc ctttctttga taaagttttc
    16621 agcattccct cgttcacgat agaattgaaa cagctcctca ctatctacac tgcttaaatt
    16681 ggtcaaatga acttttttac aaccataaaa aagagcactt aatcgtactc ttctctttta
    16741 aggattagtt gaattaattc aaatccacta gttctcctgt ttctaggtag actaggcgtt
    16801 cacaaatgtt agcaatgtaa tcagcgaagc gttccagatg ccctatgata taaaggtatt
    16861 gagttccatt gggaattgag gtttcttggt ctttcataag tccaatgatt tcctttgata
    16921 aggcataata atattggtca atctgttcat ctttttgagc aatactaata gcttttgagg
    16981 cttggtgcaa aggaaaggca accaataaat cggctagcat gctgagggat aatttaccca
    17041 tttggtgtaa ctgttcttcg tcaggggcta gttgattttc ttttagttgc aaaacagctt
    17101 tggcaatgcc tgccatatgg tctcccatac gttcaaggtc tgaacaagaa gacatgatgc
    17161 taatcacaaa tcgaaggtca gacacttgtg gctgctgcaa ggccaacaaa cgggcacagg
    17221 tcaattcgat agcgctttga ccttggttta tagcatgatc cttattgata attagctctg
    17281 ccatctcctt gtctttggag gctaaggcca gtaaggcttt tgaagctgtt tcaaggacaa
    17341 gttgccctag tcctaaaaag gattgttcta attcatgcaa ttctaagtca aattgatttc
    17401 tcataggttt ttccttcctt atccgaaccg tcctgaaata tagtcttctg tgcgctgatc
    17461 ttttggattg gtaaacacgt caacggtatc tccaaattcg caaatttctc ctgttaagaa
    17521 aaaagcagtt ttatctgaaa tacgtgaagc ttgttgcatg ttatgggtaa caatgataat
    17581 cgtataatcc ttttttagtt gctgaatgag gtcttcaatt tttaaagtgg agatagggtc
    17641 taaggctgaa gtcggctcat ccattaacag aatatcaggt tctactgcta aagctcgcgc
    17701 aatgcaaagg cgttgctgct gaccgccaga taaggacatg gcactctttt taagatcatc
    17761 tttgacttct tcccaaatgg ctgccccttt taaagatttc tccactaagg catctaattg
    17821 ttttttgtct cgaataccat gtgtccttgg gccataagcc acgttatcat agatagacat
    17881 ggcaaaggga ttaggctgtt gaaaaaccat ccctacacgc ttacgtagct gattaaggtt
    17941 gaatttgcta ctataaatat cttgctcatc taagaggact tggccttcaa tatggcaaga
    18001 aggaaccaaa tcgttcatcc ggttaagggt ttttagaaaa gttgatttgc cacaaccaga
    18061 tgggcctatc aaggcagtaa tctgtctttc tggtaattga atcgaaatat tttttaaggc
    18121 ttgaaaatcc ccgtaaaata agtctaggtg tctgactgaa aatgttccca tactaggaag
    18181 ctcctttcac aagttttcga gataataagc ttgatagagt atttatcatt aaaacagtaa
    18241 taatcaaaat cacaccggta gcataggctt cattgacatg tagcccctca cttgacagca
    18301 tatacatatg cagggctaga gaacgacctg aagacatgag actacttggc gtattggtag
    18361 aggtacctaa tgtatacatg agggcagctg tctcaccaac gatacggcca atagctagta
    18421 tcactccagc taaaatacct ggcatggcaa ctggtagaac aattctaaaa acagtccgta
    18481 acttacctgc cccaagtcca taacttgctt gacgcatgct atcactaaca gataaaaggg
    18541 cttcttctgt tgagcgaata atgactggca acaccatgat gactgaggtt aagattcctg
    18601 ataacagaga gtattgaaaa cctaagaaga ctacaaagaa gagcatgcca aacagaccaa
    18661 aaacaatgga aggaatccca gacaaggtat ctgaggccaa tcgcatgatt ttaacacaaa
    18721 gggaatcttt ttttgtatat tccacaagat aaaaaccagc aaaaatccct atgggcaagg
    18781 ctaaaagaag agcaccaaag accagaataa cggtggaaat aatcgctggc ataagggaaa
    18841 tgttctcaga agtataagtc caagaaaaga gggatagact tagatgaggt aaacctttga
    18901 tgaggataaa accaatgatt aaaaagagag agccaaaggt taaagctgaa aaacaataaa
    18961 cgagaagttt tagcaggtat ttactcataa gatgattttc ctttcaagta ggcaaagtag
    19021 gcattaatca agagaataag gaaaaagaga actgctgagg ttgcaataag ggcttcccta
    19081 tgctgacctg atgcgtaagc catttccaga acaatattgg ttgttaaggt tctggttcct
    19141 gaaaagagtc cacttggaat aatcggctgg ttgcctgcca ccaaaataac tgccatggtt
    19201 tcacctactg cgcgaccgat gcctaaaata actgctgata aaataccaga tctcgcagct
    19261 ggcaagatga cactaaaaat actccgttca tgactagctc ctagagccaa gctaccagaa
    19321 taatacgttt tgggaactgt tcggatagca gattctgaca aactgataat ggttggcaaa
    19381 atcattattc ctaatagtaa cgaagcggtt aggacactca tgccatttcc taaaaagctt
    19441 ctaatccaag gcaccaataa ttgtaggccg aaaaaaccat aaacaataga tggaatggcc
    19501 gccatcaagt tgatagctga ttttaagaag ccatagacgg gctttggaca ataataaacc
    19561 ataaacaccg atgtcaagat gcctgttggc accccaatca caatcgctcc taaggtaatt
    19621 aataaggaac caacgatcat tggtaaaata ccatagcttg ccggaatgtt cgttggcgac
    19681 caatcactgc ctaataaaaa acgggcaaag ccgtagttag ccatgaaagg taagccatta
    19741 ctaaaaataa agaaacagat tagcaaaata gctacaacag ctactgttgc actcatgaaa
    19801 aaaattgccc taaaaactgc ttctttgaag gcttgtttta tcacatcttg tcctttctag
    19861 tgaagaaagt aagggagata cgacacctcc ctacttgcct tctttatctt attgtacgat
    19921 gaaacgtctg catctcttta gagatttatg gagcaaacat tttatttaat cttgtcccag
    19981 gtggttaatt tgccactaaa aacgtctgca agttcagcca tactgacttg gcttgcctta
    20041 ttgtcattat tgaccacaac agcaataccg tctaaagcaa tagcatcatg ggtgagactc
    20101 ttaccttctt caggagttaa ttccctagaa accataccaa tatcagcggt tttctcctta
    20161 acagcggtaa tacctgctga agacccatta gaggtaatat caatcgtaac ttctggattt
    20221 tcttttttat aagcttctgc taatttttcc attaaagaag atactgaagt ggaacctaca
    20281 acagacaact tgcctgataa gtgttggctt gtatattctg tggtttcagt tttagcttca
    20341 ataaatttat tatctgtgac cacttgttga ccttgtttgg agtggataaa gctgataaaa
    20401 tcttgaccta gcttggaaag attagaagac caaacaatgt tgaagggacg ttgaagaggg
    20461 tattcaccat ctaaaactgt gtctcgacta gccttgacac catcaatctc taaagccttg
    20521 acagatttcg ttaaagatcc caaggagatg tagccgatag cattagcatt cccttgaact
    20581 gctgagagaa caccttctgt actattttga atcacagctg ttttggcagt gttgtcaatt
    20641 tttttatcac cgtctttttt gagaatccct gtgatttctg tgaaggcacc ccgtgttcca
    20701 gagccatttt ctcgtgaaat cacctcaatc gttcctgaag ctgactgttt ggaagcagct
    20761 gactgattgc cacaggcaac aagcccaaat cctgataagc caatggctgc aagagtaagc
    20821 atttttttga atttcataat aatcaccttt atctctatgt atttttcttg tgtaggctta
    20881 ctacatttat agtctaacaa gtctttgtaa aggtttatcc ctgattcatg taaagattgt
    20941 gtaaagaatc aaaaaaagcc acttttgaaa aatggctgct cctaaaaata gctttaaaaa
    21001 ttattagtcc tgtgcgaaag attggttagg aagaaaaatc gtgaagcaac tgcctctgcc
    21061 aagctgactc gtcaccgtga cttggccacc taataattga ctgagttctt tgacaatggc
    21121 aaggccaaga ccagtgccac cagtttgtct gcttcgacct ttattaactc ggtaaaaacg
    21181 ttcaaaaata cgatcctgct ctaattgact aataccaatc cctgtatctg atacagaaat
    21241 cttaatgcct tcgttcacct tttgggtctt gacctcaatt tttccccctt gttcagtgta
    21301 acggatggca ttggataaaa gattgagtaa gatttgggaa agtaattgac tatctgatac
    21361 gagggtgaca tcatctggca cctgcacctt tagctgtaaa tccttcttct tgagctgagg
    21421 ttgcaagctt tgagtcaaat cctgtacaaa ttcagccaaa gaaagggtcg tccattgtat
    21481 aggcatttgt tgagccttag ataaggtaag aagatgctca acaatatgct caagacgcaa
    21541 actttctttg taaataatgt ctagaaagtc atccttgagc gcttcttctt cagctgacat
    21601 ccccttaatg gtttcagcaa agcccttaat tgaagtaact ggtgtcctca attcatggga
    21661 ggcatttgag acaaaggcta aatttaactt ttcataagtt ctaatcgttg ttaaatcata
    21721 tagcaagacg agcacagctt ccacagattg ggtggggcta aaaacgggaa ctgctgtcac
    21781 ttctaaaatc aagtcaccct catgaaaccc acttacttct tgttttaacc ttgttttttg
    21841 atcaaaggct tggtgaacta aattccgaat atccatccgt ttgaggtcat caagtgaact
    21901 tatgtcgccg tccacatcgg gaaaataatg aggcagagag cgactggata ataacatctg
    21961 accttgagcg gaaactaaaa acgtccccat ggttaggtgc gacagaagaa cctccattgt
    22021 ttcggctagg tccttgtatt gctgatcctg ttgggagact ttggttttta ggccagacac
    22081 atactgagcc aaagacttta agtcttcttg ccctttttct aaaaagtatt cactactggt
    22141 caagagaggt tggtgcaagg tctcaaaagc aacttcccat ttccaaaggc aaaagagcca
    22201 gtagccacct tgtcccaaag aaagggctag aagaaagaga ccgatgcctt tactgatcca
    22261 agttaatgcc atccctgcaa tcagaatgag gctaacactt agattgacta gccaaaattg
    22321 aaggtagcgt ttcatctata actccttgaa cttataacca taaccccgaa tggttcgaat
    22381 aaattgaggg gctttaggat tgtcttcaat tttttccctc aacttaccaa tatgaacgtc
    22441 caccaaacgt gtttcctgcc caaagtcata cccccagata cgttccaaaa gacgctctct
    22501 agtcagtgtc atgttgggat gtttcataag atagagcaag agttcaaatt cttttggggt
    22561 caaactcagt aacttattcg ccttgtagac ttcatgacgc tcagggtata ctttcaaggt
    22621 cccaaatagc caagaatcgt cagcgatatt atctgaatca tctccttctt gttctccttt
    22681 agttcgcctg aggacagcct tgacacgcgc cagcaattct ctagggctaa aaggcttggt
    22741 caggtagtca tcagccccta attccaaggc caaaacctta tcaaattcat cacttttcgc
    22801 agaaaccatc ataattggag ttttgacgcc tttggctctc agccgcttac aaacttccat
    22861 gccatctaat tgtggtaaca tgatatcaag caagataaaa tcaaagggtt ctgtttctgc
    22921 caaagctaag gccttccgtc catttgttac caattgagta gaaaagcctt ccttacttaa
    22981 atggtagtca agcaatttca gaatgtgttc ttcatcatcc actaataaga cttgttttgt
    23041 catctattat ctcctattgg taacattata acacaattat cagaaatcct aacattgcta
    23101 aatcagatta aatttgctta tcaagactag tatctggtca aacgctcaat catctccttg
    23161 tgctctggat aggtcgccag tagatctacc ctttcaaata attcaaaatc ctcaaattca
    23221 aaaccaggag caacaagaca agaaaccaga gcataatcct tatcaactgt tgatccccaa
    23281 atagtgccct taggaacaca gtagtgaagt tgttgccctt tggatatgtc caggcctaaa
    23341 gtgactgctt cgtagtgacc atctgctgta atcatgtgaa cagtaagtgg ggatcctgca
    23401 tgaaaatacc agatttcatc tgctgtcaat cggtgaaaat gtgaaggatt cgtttcttct
    23461 aataagaaat aaatactggt ataaagcgcc cttcccttac cagcaaggtt tatagtgtct
    23521 gaagcttttt ttgtttgtct aaaatagcca ccttcaacat ggggagctaa ctctagagtt
    23581 cttatcaagt cttctttatc catcggggcc aatgggttga agtaactctt gttcaaagtg
    23641 gttttacgat ttcaagaact cctctcagtt ctgaggacac ggtaatgatt gatgcgacgg
    23701 aagtacaaat caatcgccct aaaaaaagaa ttagcgaatg attctggtaa aaagaaattt
    23761 cacgctatga aggctcaagc gattgtcaca agtcaaggga gaattgtttc tattgccatg
    23821 atatgaagtt gttcaaaatg agttgcagaa atatcggaca agctggtaaa atcttggctg
    23881 acagtgatta tcaagggctc atgaagatat atcctcaagc acaaactcca cgtaaatcca
    23941 gcaaactcaa gccgctaaca gctgaagata aagcctataa ccatgcgcta tctaaggaaa
    24001 gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt taaaatattt tcaacaacct
    24061 atcgaaatca tcgtaaacgc ttcggattac gaatgaattt gattgctggt attatcaatc
    24121 atgaactagg attctagttt tgcaggaagt ctattatttg gttaggtgaa ttagtgaagt
    24181 gtttaggcaa gtgtctctgg ttacgacgtc atggcctcta aatcgattat atttaggggt
    24241 catgactagt gaagcagtta gctagttcgc atataagcgg ctagcgtcta acaattagga
    24301 actttagttc caataacttt aagattacga cgttttagga cataaatcga tcatatttat
    24361 gtcctaaaac tagtgaagcg cctagccaaa gtccgaatag gatttggcgt tagttactta
    24421 gattgctttg caatcaagta actttggcga tttacatctt ctctggcgct tctactccaa
    24481 gcaagcgaag ggcttctttg agaacgactg cggttgcgta gctgagggct agacggctgt
    24541 cgcgttctgg gctttcatcc aagatacgtg tatgtgcata gtatttgtta aaggattgag
    24601 ccaggctaat tgcaaattta gcaatgatag aaggttcaaa gttatctgcc gcacggttga
    24661 taatacgtgg gaagtcttga atgagtttaa tgatttccca gctttcagta tcattcaagc
    24721 tatagttgcc agctgtttct ggtttgaaat cggctttgcg taagatagat tggatacgag
    24781 cgtaggcata ttgaacgtaa ggtccagttt caccctcgaa ggataccata gcttctaggt
    24841 cgaagtcgta tccatttgta cggtcagttt tgaggtcata gaatttaatg gctccaaccc
    24901 caacagcatg tgctacttgg tctttgtttt ctagttcagg atttttagcc tcgatttgga
    24961 ccttggcacg gctaacagcc tctgcaacag taggctctag caagatgaca ttccctttac
    25021 gagtagagag cttcttccct tcttttgtaa ccagaccaaa aggaacgtgg gtaatgtcgt
    25081 cactccaatc gtagcccatc tcttgcaaga cagctttgag ttgtttaaag tgggcagatt
    25141 gctcttgacc aacgacgtag atagatttag caaattggta ttcattttta cggtagaggg
    25201 ctgcagccaa gtcacgtgtg atatagagag ttgcaccatc agacttcttg atgagggctg
    25261 gatgttcaat tccatatttc tcaagattca caacttgggc accttctgat tcaagaagta
    25321 gtcctttttc agaaagaatg tctacaactg catccatctt atcattgtag aaggcttctc
    25381 cgttatagct gtcaaattca accttcaatt cattgtaaag gcggttaaat tccactaaac
    25441 tttcatcgcg gaaccattgc caaagagcga gagcttcctc atctccattt tcaagtttac
    25501 ggaaccattc gcgcgcttct tcatccaagc tagggtcatt ttcagcttca gcgttgatgc
    25561 ggacatagag tttaaggagt tcatcgattg gatgagcttt tacagcttct tcgtcgcccc
    25621 attttttgta ggcaacaatc aacatcccaa attgtttacc ccagtctccc aaatggttga
    25681 ccttgaccgt ttgataaccg attttttgga aaatatgtga caagctatct ccgataacag
    25741 ttgaacgcag gtggccaata gaaaatggtt tagcgatatt cggactagac atgtcgataa
    25801 caacattttc ttgtttgcca atattttggt cagcatagtg ttctttttca gtggtaacag
    25861 cttgcaatac ttgagcagaa atggcagatt tatcaaggaa aaagttaacg taaggtcctg
    25921 ttgcgacaac tttttcaaag gcttggctgt tcattttttc agccagttca gccgcaatca
    25981 tttgtggtgc tttacgttcg actttggcaa gagaaaaagc agggaaagca atgtctccca
    26041 tttctgagtt tttaggggtt tccagtaact ttaaaatagc ctcttggtcc aggctatcaa
    26101 tgatgctaga taattcgcta gcaatcaatt cttttgtatt cattaagagc tcctttttgg
    26161 acttttctac tattttatca caattttaaa gaaagaagaa aaaatttttg aaatctcctg
    26221 tttttttggt ataatatggt tataaatata gttataaata tgcacgcaag aggattttat
    26281 gagaaaaaga gatcgtcatc agttaataaa aaaaatgatt actgaggaga aattaagtac
    26341 acaaaaagaa attcaagatc ggttggaggc gcacaatgtt tgtgtgacgc agacaacctt
    26401 gtctcgtgat ttgcgcgaaa tcggcttgac caaggtcaag aaaaatgata tggtgtatta
    26461 tgtactagta aatgagacag aaaagattga tttggtggaa tttttgtctc atcatttaga
    26521 aggtgttgca agagcagagt ttaccttggt gcttcatacc aaattgggag aagcctctgt
    26581 tttggcaaat attgtagatg taaacaagga tgaatggatt ttaggaacag ttgctggtgc
    26641 caatacctta ttggttattt gtcgagatca gcacgttgcc aaactcatgg aagatcgttt
    26701 gctagatttg atgaaagata agtaaggtct tgggagttgc tctcaagact tatttttgaa
    26761 aaggagagac agaaaatggc gatagaaaag ctatcacccg gcatgcaaca gtatgtggat
    26821 attaaaaagc aatatccaga tgcttttttg ctctttcgga tgggtgattt ttatgaatta
    26881 ttttatgagg atgcggtcaa tgctgcgcag attctggaaa tttccttaac gagtcgcaac
    26941 aagaatgccg acaatccgat ccctatggcg ggtgttccct atcattctgc ccaacagtat
    27001 atcgatgtct tgattgagca gggttataag gtggctatcg cagagcagat ggaagatcct
    27061 aaacaagcag ttggggttgt taaacgagag gttgttcagg tcattacgcc agggacagtg
    27121 gtcgatagca gtaagccgga cagtcagaat aattttttgg tttccataga ccgcgaaggc
    27181 aatcaatttg gcctagctta tatggatttg gtgacgggtg acttttatgt gacaggtctt
    27241 ttggatttca cgctggtttg tggggaaatc cgtaacctca aggctcgaga agtggtgttg
    27301 ggttatgact tgtctgagga agaagaacaa atcctcagcc gccagatgaa tctggtactc
    27361 tcttatgaaa aagaaagctt tgaagacctt catttattgg atttgcgatt ggcaacggtg
    27421 gagcaaacgg catctagtaa gctgctccag tatgttcatc ggactcagat gagggaattg
    27481 aaccacctca aacctgttat ccgctacgaa attaaggatt tcttgcagat ggattatgcg
    27541 accaaggcta gtctggattt ggttgagaat gctcgctcag gtaagaaaca aggcagtctt
    27601 ttctggcttt tggatgaaac caaaacggct atggggatgc gtctcttgcg ttcttggatt
    27661 catcgcccct tgattgataa ggaacgaatc gtccaacgtc aagaagtagt gcaggtcttt
    27721 ctcgaccatt tctttgagcg tagtgacttg acagacagtc tcaagggtgt ttatgacatt
    27781 gagcgcttgg ctagtcgtgt ttcttttggt aaaaccaatc caaaggatct cttgcagttg
    27841 gcgactacct tgtctagtgt gccacggatt cgtgcgattt tagaagggat ggagcaacct
    27901 actctagcct atctcatcgc acaactggat gcaatccctg agttggagag tttgattagc
    27961 gcagcgattg ctcctgaagc tcctcatgtg attacagatg ggggaattat ccggactgga
    28021 tttgatgaga ctttagacaa gtatcgttgc gttctcagag aagggactag ctggattgct
    28081 gagattgagg ctaaggagcg agaaaactct ggtatcagca cgctcaagat tgactacaat
    28141 aaaaaggatg gctactattt tcatgtgacc aattcgcaac tgggaaatgt gccagcccac
    28201 tttttccgca aggcgacgct gaaaaactca gaacgctttg gaaccgaaga attagcccgt
    28261 atcgagggag atatgcttga ggcgcgtgag aagtcagcca acctcgaata cgaaatattt
    28321 atgcgcattc gtgaagaggt cggcaagtac atccagcgtt tacaagctct agcccaagga
    28381 attgcgacgg ttgatgtctt acagagtctg gcggttgtgg ctgaaaccca gcatttgatt
    28441 cgacctgagt ttggtgacga ttcacaaatt gatatccgga aagggcgcca tgctgtcgtt
    28501 gaaaaggtta tgggggctca gacctatatt ccaaatacga ttcagatggc agaagatacc
    28561 agtattcaac tggttacagg gccaaacatg agtgggaagt ctacctatat gcgtcagtta
    28621 gccatgacgg cggttatggc ccagctgggt tcctatgttc ctgctgaaag cgcccattta
    28681 ccgatttttg atgcgatttt tacccgtatc ggagcagcag atgacttggt ttcgggtcag
    28741 tcaaccttta tggtggagat gatggaggcc aataatgcca tttcgcatgc gaccaagaac
    28801 tctctcattc tctttgatga attgggacgt ggaactgcaa cttatgacgg gatggctctt
    28861 gctcagtcca tcatcgaata tatccatgag cacatcggag ctaagaccct ctttgcgacc
    28921 cactaccatg agttgactag tctggagtct agtttacaac acttggtcaa tgtccacgtg
    28981 gcaactttgg agcaggatgg gcaggtcacc ttccttcaca agattgaacc gggaccagct
    29041 gataaatcct acggtatcca tgttgccaag attgctggct tgccagcaga ccttttagca
    29101 agggcggata agattttgac tcagctagag aatcaaggaa cagagagtcc tcctcccatg
    29161 agacaaacta gtgctgtcac tgaacagatt tcactctttg atagggcaga agagcatcct
    29221 atcctagcag aattagctaa actggatgtg tataatatga cacctatgca ggttatgaat
    29281 gtcttagtag agttaaaaca gaaactataa aaccaagact cactagttaa tctagctgta
    29341 tcaaggagac ttctttgaca attctccact tttttgctag aataacatca cacaaacaga
    29401 atgaaaagga gctgacgcat tgtcgctccc ttttgtctat tttttaagga gaaagtatgc
    29461 tgattcagaa aataaaaacc tacaagtggc aggccctggc ttcgctcctg atgacaggct
    29521 tgatggttgc tagttcactt ctgcaaccgc gttatctgca ggaagtctta ggcgccctcc
    29581 ttgctgggaa atatgaagct atttatagta tcggggcttg gttgattggt gtggccgtag
    29641 tcggtctagt tgctggtgga ctcaatgttg tcctcgcagc ctatattgcc caaggagttt
    29701 catccgacct tcgggaggat gccttccgta aaattcaaac cttttcttat gctgatattg
    29761 aacaatttaa tgcgggaaat ctagtcgttc gaatgacaaa tgatatcaac cagattcaga
    29821 acgttgtcat gatgaccttc cagattcttt tcagacttcc cctcttgttc atcggttcgt
    29881 ttatcctagc ggttcaaacc ttaccttctc tgtggtgggt gattgttctc atggtagtct
    29941 tgatttttgg tttgactgct gtcatgatgg gaatgatggg gcctcgtttt gccaagtttc
    30001 aaacccttct tgagcgcatc aatgccattg ccaaggaaaa tttacgtggc gttcgtgtgg
    30061 tcaagtcctt tgtccaagaa aaagagcaat ttgctaagtt tacagaggtc tcagacgagc
    30121 ttcttggtca aaacctttac attggttatg ccttttcagt agtggaaccc tttatgatgt
    30181 tggttggtta cggggcggtc ttcctctcta tttggctggt cgcgggaatg gttcagtcgg
    30241 atccgtctgt tgttggttcc atcgcttctt ttgttaatta cctaagccag attatcttta
    30301 ccattgttat ggttggattt ttgggaaatt ctgtcagccg tgccatgatt tccatgcgtc
    30361 gtattcgaga aattcttgac gcagagccag ctatgacctt caaggatatc ccagatgaag
    30421 agttggttgg aagccttagc tttgaaaatg tgacctttac ctatccaatg gacaaggaac
    30481 cgatgctgaa agatgtgagc tttactattg aacctggtca aatggttggt gtagttggag
    30541 cgactggtgc aggaaagtca accttggctc aattgattcc acgtctcttt gatccacagg
    30601 aaggggctat caaaatcgga ggtaaggata ttcgagaagt gagtgaagga accctgcgta
    30661 aaacagtttc catcgttctc caacgtgcca ttctttttag tggaacgatt gcagataact
    30721 tgagacaggg gaagggaaat gctactctat ttgaaatgga gcgcgcagcc aatattgccc
    30781 aggctagtga attcattcat cgtatggaga aaacctttga aagtccagtt gaagaacggg
    30841 gaaccaattt ctctggtgga caaaaacaaa ggatgtcgat tgcgcgtggg attgtcagca
    30901 atccacgtat tctgattttt gatgattcga cctcagcctt ggatgccaaa tcagagcgct
    30961 tggtgcaaga agctttgaat aaggacttga aggggacgac aaccattatt attgctcaaa
    31021 aaattagctc ggttgtccat gcagacaaga tcttggttct aaatcaagga cgattgattg
    31081 gtcaaggcac gcatgcagac ttggttgcca acaatgccgt ttaccgtgaa atctatgaaa
    31141 cacagaaatg aaagacaaac tataagaaaa gtcaatagtt ttatctaaac tatttcttat
    31201 ttcaatttga tgatttggcg atgattttag agcacggcaa aaagcccttg aaaaagtcca
    31261 ttttttcaaa ggtaatcctg tgttaatttc agaaattaca tcactttttg ttcgtcaaat
    31321 ggcagctctt ttttaggata taaaacaggg ttcggataag tttttttgca aggtggatga
    31381 tggctacatt gtaatgtttt ccttgttcta acttagtctt aagataggcc t
//
LOCUS       DAVKFH010000024        22205 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKFH010000024 DAVKFH010000000
VERSION     DAVKFH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.024"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(14..211)
                     /gene="blpI"
                     /locus_tag="VW006_001134"
     CDS             complement(14..211)
                     /gene="blpI"
                     /locus_tag="VW006_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9379998.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            493..1080
                     /locus_tag="VW006_001135"
     CDS             493..1080
                     /locus_tag="VW006_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9379999.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1007..2650
                     /locus_tag="VW006_001136"
     CDS             1007..2650
                     /locus_tag="VW006_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9380000.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2661..4022
                     /locus_tag="VW006_001137"
     CDS             2661..4022
                     /locus_tag="VW006_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9380001.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4079..4207
                     /gene="blpC"
                     /locus_tag="VW006_001138"
     CDS             4079..4207
                     /gene="blpC"
                     /locus_tag="VW006_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9380002.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4278..5618)
                     /locus_tag="VW006_001139"
     CDS             complement(4278..5618)
                     /locus_tag="VW006_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9380003.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5632..6369)
                     /locus_tag="VW006_001140"
     CDS             complement(5632..6369)
                     /locus_tag="VW006_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380004.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6374..6712)
                     /locus_tag="VW006_001141"
     CDS             complement(6374..6712)
                     /locus_tag="VW006_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380005.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6988..7317
                     /locus_tag="VW006_001142"
     CDS             6988..7317
                     /locus_tag="VW006_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9380006.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7485..8534)
                     /locus_tag="VW006_001143"
     CDS             complement(7485..8534)
                     /locus_tag="VW006_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380007.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8531..9262)
                     /locus_tag="VW006_001144"
     CDS             complement(8531..9262)
                     /locus_tag="VW006_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380008.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9330..9740
                     /locus_tag="VW006_001145"
     CDS             9330..9740
                     /locus_tag="VW006_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9380009.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9750..10037
                     /locus_tag="VW006_001146"
     CDS             9750..10037
                     /locus_tag="VW006_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380010.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10199..10483
                     /locus_tag="VW006_001147"
     CDS             10199..10483
                     /locus_tag="VW006_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380011.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDTTDEVSSKHCFEVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(10795..11931)
                     /gene="dnaJ"
                     /locus_tag="VW006_001148"
     CDS             complement(10795..11931)
                     /gene="dnaJ"
                     /locus_tag="VW006_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9380012.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12333..12689)
                     /locus_tag="VW006_001149"
     CDS             complement(12333..12689)
                     /locus_tag="VW006_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9380013.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVPAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12691..14514)
                     /gene="dnaK"
                     /locus_tag="VW006_001150"
     CDS             complement(12691..14514)
                     /gene="dnaK"
                     /locus_tag="VW006_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9380014.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14994..15518)
                     /gene="grpE"
                     /locus_tag="VW006_001151"
     CDS             complement(14994..15518)
                     /gene="grpE"
                     /locus_tag="VW006_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9380015.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15545..16579)
                     /gene="hrcA"
                     /locus_tag="VW006_001152"
     CDS             complement(15545..16579)
                     /gene="hrcA"
                     /locus_tag="VW006_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9380016.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16743..17252)
                     /locus_tag="VW006_001153"
     CDS             complement(16743..17252)
                     /locus_tag="VW006_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9380017.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17860..20193
                     /locus_tag="VW006_001154"
     CDS             17860..20193
                     /locus_tag="VW006_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9380018.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAMVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20206..21669
                     /locus_tag="VW006_001155"
     CDS             20206..21669
                     /locus_tag="VW006_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9380019.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21669..>22205
                     /locus_tag="VW006_001156"
     CDS             21669..>22205
                     /locus_tag="VW006_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9380020.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFI"
BASE COUNT         7062 a         4502 c         4097 g         6544 t
ORIGIN      
        1 aagaatcata atcttacaaa gaggacataa ttcctgaacc tacacaaata agtgttgctg
       61 ctcccccagt tatcggacca gtcgcagcag ctaatagtac tgctccaata caaccaccga
      121 ttgcagatcc taaattgcct cttcctccac taactatttc gagttcttca ttatccataa
      181 cagaaaattg ttccatcatt tttgtattca tgacaaatac tccttttttc tttttttatt
      241 tttgtcttgt tgtaactttg ataagtttag tatatcatcg ttttttaaaa tttttcatcc
      301 agatcttgaa ttgtcatcga aacgtcttga attaggtttt ttatttcaag ccacctctaa
      361 atgtttaaaa aaaataattt ctaatcactt ttttaccatt caggaagttt taatgactat
      421 tcaagatttc ataaaatatg aacttagttt tatgacataa tagacctatc cactatatga
      481 aaggaattgc caatgacttc ttataaacgt acatttgttc ctcaaataga tgcgagagac
      541 tgtggtgtcg ctgccttagc ctcgattgct aaattctatg gttcagattt ttctctagct
      601 cacttgagag aacttgcaaa gaccaataaa gaagggacga ctgctcttgg cattgtaaaa
      661 gccgctgatg aaatgggctt tgaaacaaga cctgttcaag cagataaaac gctctttgac
      721 atgagtgatg tcccctatcc atttatcgtt cacgttaaca aagaaggaaa actccaacat
      781 tactatgttg tctatcaaac aaagaaagac tatctgatta ttggtgatcc tgacccttct
      841 gtaaaaatca ctaaaatgtc aaaagaacgc tttttctatg aatggactgg agtagctatt
      901 tttctagcta ccaaacccag ctatcaaccc cataaagata aaaagaatgg tctactaagc
      961 aagcttcctt cctctgattt tcaaacaaaa atctctcatt gcttacattg ttctctcaag
     1021 cttattggtc actattatca atataggtgg ttcttactat ctccaaggaa tcttggatga
     1081 atacattcca aatcagatga aatcaacttt aggaatcatc tcagttggtc tggttatcac
     1141 ctatatcctc caacaagtca tgagcttctc cagagattat ctcctaaccg ttctgagtca
     1201 gagattaagt attgatgtga ttttatccta tattcgccat atttttgaac ttcccatgtc
     1261 tttctttgcg acacgtcgta caggagaaat catttcacga ttcacagatg ctaactctat
     1321 tatagatgcc ttggcttcta ccattctttc tctttttctg gatgtttcta ttctgattct
     1381 tgtaggaggc gtcttactgg cacaaaaccc taatctcttc cttctttctc ttatttccat
     1441 tcctatatac atgttcatca tcttttcttt tatgaaacct ttcgaaaaaa tgaaccatga
     1501 tgtcatgcaa agtaattcta tggttagctc tgccattatc gaagatatca acgggattga
     1561 aactataaag tcgctcacga gtgaagaaaa tcgctatcaa aatatagaca gcgaatttgt
     1621 agattatttg gaaaaatcct ttaagctcag taaatattct attttacaaa cgagtttaaa
     1681 gcagggaaca aaattagttc tgaatatcct tatcctatgg tttggcgctc aattagtcat
     1741 gtcgagtaaa atttctatcg gtcagctgat tacctttaac acactttttt cttactttac
     1801 aactcctatg gaaaatatta tcaacctcca aaccaaactc caatctgcga aggtcgctaa
     1861 taaccgtttg aacgaagtct atctagtcga atctgaattt caagttcaag aaaaccctgt
     1921 ccattcacat tttttgatgg gcgatattga atttgatgac ctttcttata agtatggttt
     1981 tggacgagat accttaacag atattaatct cacgattaaa caaggagata aggttagcct
     2041 agttggagtt agtggttctg gtaaaacaac tttagccaaa atgattgtca atttctttga
     2101 accctacaaa ggacatattt ctatcaatca tcaggatatt aaaaacattg ataaaaaagt
     2161 cttgcgccgt catattaatt acctaccaca acaagcctat atctttaatg gctctatctt
     2221 ggaaaactta actttgggcg gtaatcatat gattagccaa gaagatattc taagagcttg
     2281 tgaattagct gaaattcgtc aagacattga aagaatgcct atgggctatc aaactcagct
     2341 ctctgatgga gctggtctat caggaggaca aaagcaacga atagccctcg ctcgtgctct
     2401 tttaactaaa gctcctgttt taatactaga cgaagctact agcggtcttg atgtcttgac
     2461 tgagaaaaag gttatagata atcttatgtc tctaactgat aaaaccattc tctttgtagc
     2521 ccatcgtctc agtatagccg aacgaaccaa ccgtgtcatt gttcttgacc aggggaaaat
     2581 cattgaagtt ggtagtcacc aagagttaat gcaggcgcaa ggtttctacc atcatctatt
     2641 caataaataa ggagaatgtc atgaatccta atctttttag aagcgtcgag ttttatcaga
     2701 gacgttacca taactatgcg acagtgttca ttatacctct ttcattacta tttactttca
     2761 tcttgatttt ctcccttgtt gccacaaaag aaattactgt tacttcccaa ggagaaatcg
     2821 cccctacaag tgtcattgcc tccattcagt caaccagtga taatcctatc ctagctaatc
     2881 atttagtggc aaatcaagta gttgaaaaag gggacttact catcaaatac tctgaaacaa
     2941 tggaagaaag tcagaaaact gccttagcaa ctcaattaca aagacttgag aagcaaaaag
     3001 aaggacttgg aattttgaaa caaagcttag aaaaagcgac tgatcttttt tctggcgagg
     3061 atgaatttgg ctaccataat acctttatga attttactaa acaatcccat gatattgaac
     3121 tgggtatcac aaagactaac accgaagttt caaatcaagc taatctttcc aatagcagtt
     3181 catcagctat tgaacaagaa attacaaaag ttcaacaaca aattggagaa tatcaagagt
     3241 tgagagatgc tatcataaat aacagagcac gcttaccaac tggcaatccg caccagtcaa
     3301 ttttgaatcg ttatcttgta gcctcacaag gacaaacaca aggaactgca gaggagccat
     3361 ttttatctca aattaatcaa agtattgcag gtcttgaatc atctatcgca agcctcaaaa
     3421 ttcagcaagc tggtatcgga agtgtagcaa cttatgataa cagtttagca accaaaattg
     3481 aagtactccg cactcagttt ttacagacag cctcacagca acaactaact gtggagaatc
     3541 aattaacaga attaaaagta caactagatc aagccacaca gcgtttggaa aacaatacct
     3601 taacctcccc aagtaaaggt atcgttcatc tgaacagcga atttgaaggt aaaaatagaa
     3661 ttccaactgg tacagaaatt gctcaaatat tccctgtcat cacagataca agagaagtac
     3721 taatcactta ctacgtatct tctgactatc tacctctact agataaagga caaactgtaa
     3781 gattaaaact ggagaagatt ggaaatcacg gcaccaccat catcggccaa cttcagacaa
     3841 ttgatcaaac tcctaccaga acagagcaag gaaatctctt taaattaacc gctcttgcaa
     3901 aactatctaa cgaggatagt aaactcatcc aatatggctt acaaggtcgc gtcactagtg
     3961 taactacaaa gaaaacatat tttgattatt tcaaagataa aattttaacc cattctgatt
     4021 aattttcaga taacactcta taactattta ttatcttatc aaaaaggaga atcataacat
     4081 ggataagaaa caaaacctaa cttcatttca agaactaaca actaccgaac tcaaccaaat
     4141 tacaggtgga ggattgtggg aagatttatt atataacatt aatagatatg ctcattacat
     4201 cacataagaa cttcatcatc caatacaact ataaaaaaat aagaccgaga aacaagtact
     4261 ctcggtctta tttttcatca ttctgtatgt atcacagtaa gtacctgacg aaagacttga
     4321 ttttgacagg tggtatttag actggtatta ggatggcttt ccacaatctt catgacggta
     4381 tagagaccaa ctcctctctc ctccccttta gaactggctc caaaggagaa gatttcagaa
     4441 atatcgatgc cctcttcttt gatggagttt tcgatgataa aggtctcctg tgctccattt
     4501 tttaaaaagg cgattgaaac atgaggttga ctagcttcca cactggcttc aatagcattg
     4561 tcacaaagga tagacacaat ggttagaaaa tcaagtagac tcatcccctc gacctgaatc
     4621 tcctcaggaa cttcgacatt aaagacaatg ttcttatctc tggcttttaa aaatttccct
     4681 gctagaagac ttttgagggc tttatcacga atatttacca atctgcccag gtcatattta
     4741 ttgttctgca atttctgact ggaatccttt aagacggagc catagacctc ttttatctgc
     4801 tccatatcct cctcttcaat gcccagacgt aagctagtca agaggttggt ataatcatga
     4861 cgaaagctcc gtacttcctt gtaaagctcc tctatatgcc gactatagcg ttccatatct
     4921 ctatagcgca gggcctgctc ttgttccaat ctctcataga gtttttcctt caaataggta
     4981 tccaatttct tgataacccc cataaaaaag agtaggtaaa agactaggat gagatggcga
     5041 acagtctttg attgaatact ttgttcatat tcaaaaaaag acagactttc catgactaga
     5101 tagtagccac ccattatcca gttaatctga gtcagggact tttgaaaggc tttatcgaga
     5161 atctcctttc tcaagctagt aaaatcgtag tccaaccatt tcaaaaaagc tagagaaatg
     5221 aagaaattga aaattattat acataaccca gtaaatgagt agccatcata tacttgccct
     5281 tgtcccaaaa atggaagcac aaaataggag actcctctat aaaagagatt caccaatatc
     5341 attggaaaga gaccataaaa gaaaaggagt tttttaggaa gccctctcaa taataagaaa
     5401 gataagccta tgccgtacaa gggttccata aaataagata ggtaaacatt tcctactata
     5461 tagctaatca tcacaaaaac aaaggccaac agtatcttaa aaagaaaggc cttaaaaatc
     5521 ctctcgaaag taagatcaat tccatccacc ttaaagaaga tgacaatttc tagtccatta
     5581 gtaacaagtg tatacaacaa tatccaagca atgttcataa attctcctag ctcagtgtaa
     5641 tttattgatg gcctcagaca cttccctgac cttataacgg gcgattagac aacttccacc
     5701 attgggaaag aaaagcagtt tttctttctt atccaaatgc accacatttg caggattgat
     5761 gagaaaagag cggtggcact gcaagagacg gggctcctgc ttgaaaacct cctctaaact
     5821 cgctgtaaat tccagcctgt ctgtcttggt atagagaata acacgatggg ctctgggcga
     5881 cgtttcgaga tagtaaacct ctttaaaagg atactgaaat tgggcaaatt ttgatttaaa
     5941 gtaaaagcaa tcttccgcca gacttttact atcttgacta ttggcataga ggagggctgt
     6001 ctcgatccga gattcaaact cctctgctga caaggcctta tcaatgtagt ccaaagcaga
     6061 cacttggtag cgaaaagaca ggggcataaa ctccgagtga gtcgtcacaa agacaatcag
     6121 ggcataagga tcccgatccc gaatctttct agccacttcc agtcccttca tctcttcatt
     6181 tcgaatctca atatccaaaa agaatagctg atgggccccc ttctcatgca cttcagccag
     6241 cagttggtcc ggcttgccaa atacctcaaa agagctagga atgatatgat gtgctttcaa
     6301 aagtttctca atcgtcgttt caattctagt ctgttgggaa aaatcatcct ctaaaacaaa
     6361 tattctcatc ttcttactct ccctctttca accatttttg acgaatttct ctatagcgac
     6421 gtctggcata cttgacagcg tattgacctt cttctccgag aaaaaggatt ctttcttgaa
     6481 aatcaatcga ttttaattta tcaagattaa ccaaacaatt tcgatgacat ctcatcaagg
     6541 tttccccaca ttggttctca agattactta aattttgaag catattaaaa ctagcttctt
     6601 ctgtaacaat ctgtacggta tgggctttag ttggatgtgt ttggatatag tagatatcgt
     6661 cgatgtttac tttatagact gttttctgcg tctgaataat catatatttc attggattct
     6721 cctcctaaga aaagaataac agaaaaggtc tagcccaagc acacccaatc ctaaacagtc
     6781 tttgaaaact cctaaaagga ttattttcta caacatcatg atgagcttac aactatttta
     6841 tcataaaatt cactcagcgc aattcaagac atttcaatga caattaaaga tttgaataaa
     6901 aaaaaagaat taaaagtgat aaactaacag tatcaaaaca cagttgccag aagtaagact
     6961 ggcacccaga ttaaaggagg cattcccatg acagatacag accccatcaa aagagctcat
     7021 actttgatta ctgacttaaa caaagcctac caagcatgca aacaggcaag cgctgacgac
     7081 gtccgctttc aggagcaatt aaactctatt cttggttttc tagccaaggc tgaaacagtg
     7141 gataatcgat tcttgattga actagaaaaa ttttaccaga cttccagtct tctcatggga
     7201 ctcagcgctc ttgatccaga tgctccaact cgcgccgctt ggcgggccta tgaccgcttc
     7261 cactttgacc aagtcaagac caagttaata ctcaatgaaa atcaaagagc aaactaggaa
     7321 gctagccgca agctgtactt gagtacggta agacgacgct gacgtggttt gaagagattt
     7381 tcgaagagta taagtctcta cggaccaacc attatcctgt agtaacaaaa aagaagttga
     7441 gacagactga actcaacttc ttttttagtg tcgtatcagc aatgttagtc ctgcatctga
     7501 cgttttacct gataaggcaa atacaagact agtaaaacca aaccagctcc tagtaaggct
     7561 agaagggcta gtttttcttg gaaggtaatc aacccaacaa ctaattccac aagtaaaaca
     7621 aaactggtca atcctcgaac tacctcctgt aagccttttt ccttttgccc cttgtccagc
     7681 ggaaagagtt gggtcaaata ctggtagtca aaggcatgat agagggccag caactggaag
     7741 agcaagaggt agttaaagag aactaccact gctgtcgcaa tccaagcttg ctcgataaaa
     7801 acctgcgcca gtacggaaag taagagaaga cgaagactga gagcaaagag gtcgccattt
     7861 cgcagataag aacgcagata gagattttgc caaatctttc cagacacctt ctgaacagcc
     7921 tttaagataa agtccagata ggcacgacgt ttgacactgt ttgaaattcc ctttacctgc
     7981 gtaaagaggg caaagaaacg aagcaagact tgcttacgct tgctttcttg ggaaatgaca
     8041 tagtcccagt ccagcccagt ttcagtgaaa aatttgctgg ccttttgacg aaagtggaaa
     8101 tattttccta cccccaataa aagcacatag agcagaaaaa ctggcaagcc ataacccatt
     8161 gctaaaaata aaggcgcaaa taacagcaag aaaagggtct gtacaaagag ccaaaagact
     8221 agggaaatgc cagtttgacg cttgagatgg agcttgattt cctcttctcc aactaagaga
     8281 aagagcttgt ctggagcctc catataggtg gcaattcctc cccaaagtaa aagtaaaaca
     8341 gacgtaattc ctacaaacaa aaggataggc caatgatttt caggaaaatg ttgtaagagt
     8401 tgactgtact ggtaggctag aaagcccaac aggacaagca ggaacaagac aaagtggtca
     8461 ttgagcacat agcgcagata accaaggcac tccttacgaa aggcctgctt tctctttaaa
     8521 aacaagtctt tcatagatcc tcctctttgg tcagagccaa gtaaatatca ttcaaactag
     8581 cctcaggcat atcaaaggct tcacgtagtt gcaggagatt gcctttggca cgcacctctc
     8641 ccttgtgaag aatgacaaag gcatcacaca tcttctccgc cgaatccagc acgtgggtac
     8701 tcatgagaat agacttgccc ttttgcttct ccacttccaa aagctgaatc aaatcagaaa
     8761 tagccagcgg atcaagacca aggaaaggct catccacgat gaaaagactt ggatccacca
     8821 caaaagcaca gataatcatg accttctgct tcatcccttt tgaaaaatga acagggaacc
     8881 agtctaattt ctgttccaaa cggaacattt ttaacaaggg ctctactcgt tcgaaagcca
     8941 ctttttgctc aataccgtaa gccatagcaa ccgtttcgat atgctctctg agggtcaatt
     9001 cctcatacag actaggcgtc tcaggaatgt agccaatctg cttgcggtag ctagtcgcat
     9061 ctccttgcag agtcaggcca ttgatattga tggagccact ataaggtgcc aacagaccga
     9121 taatctcatt gatcgtcgtt gatttcccag caccattgag accaatcaaa ccgaccaact
     9181 gcccactttc aacagtaaag gacacatctt tcaaaacagg gacatgaaca tagccacctg
     9241 tcaggttttt aatttctaac atattttctc caaatctggt ataatgtagc tatattatat
     9301 caaaattcag tacagtagag gtagatttta tgtcagattg cattttttgt aaaatcatcg
     9361 caggggaaat tcctgcttca aaagtatatg aagatgagca ggtccttgcc tttcttgata
     9421 tctctcaagt aacactagga cacaccttgg tcgtgccaaa agaacactat cgcaatcttt
     9481 tggagatgga tgctacgagc gccagccaac tctttgccca agtaccaaaa gtagctcaaa
     9541 aagtcatgaa agtcactaag gctgctggta tgaatatcat ttccaactgt gaagaagtcg
     9601 ctggtcaaac agtttttcat actcacgttc accttgtacc tcgctacagt gctgacgatg
     9661 acctcaagat tgattttatc gcccacgaac cagactttga caaacttgct caagtcgctg
     9721 aaaccatcaa aaacgcttaa ggagttgcta tgaaattatc caacctactg ctatttgcag
     9781 gagctgcagc cggaagttat ctggtcacaa aaaatcgcca aaccatcaca gatgaagtct
     9841 tgaataccac tgaccgcgtt caagctatca aggacgatgt ggatattatc caaaacagcc
     9901 tgcaaatcat tgaccagcaa aaagaactta tcaaggaata ccaagaagac ttgacttaca
     9961 agtttaaggt cttggaaaag gatatccaga ctagactagc tgtgataaaa gaaatgcagg
    10021 gaactgaaga taagtaaaaa gagccccccg gctctttttt aaaacgtaaa caagttatag
    10081 tgaattgaat ttagaatagc acatcatagt ttctaaagca ttgttagaga ttactttaaa
    10141 atcctcttat caatttgttc atattctatt tcaatctact atatatatcc catcaactat
    10201 gcatcataat ttaggtaact catactcaat aaaaatcaaa aagcaaacta gaaagctagg
    10261 cacagactgc tcaaaacacc gttttaaggt tgtggataga actgacgaag tcagctcaaa
    10321 acactgtttt gaggttgtag atacgactga cgaagtcagc tcaaaacact gttttgaggt
    10381 tgtggataga actgacgaag tcagtaacca tacccatgac aagccgacgc tgacgtggtt
    10441 tgaagaaatt tttgaagagt atcattcacc atttcacaac taaatcgaag actttctgcc
    10501 gtaatgaaaa cacctgaaac agcttggttt cggctgtccg gaaactttaa gacttaggtt
    10561 caaagtttag gtatggaact tcgaaggagg tcgctaccgt ccgtcattac ttaggaaagt
    10621 cttaaaaaat cggctctata aagtaaaaag agcccgacag ctctttttgt ttatattcaa
    10681 tgaaaatcaa aaagcaaact aggaaactag ccacgggctg ctcaaaacac tgttttgagg
    10741 ttgtagatac gacaagccga cgctgacgtg gtttgaagaa atttttgaag agtattattc
    10801 tccatcaaag gcatctttaa tatggtcaaa gaagcctttt ttctttggat ttactttcaa
    10861 gtcaccagca gccgcgaatt ctttcaaggc tactttttgg cggtcgttca agcctgtcgg
    10921 tgttacgaca ttaacagtaa cgtattggtc accaactgca ccgccacgaa ggctcggtgc
    10981 ccccttacta cgtaggcgga atttcttacc agtctgagtt ccctctggaa taaccaattc
    11041 aacatcaccg tgaacagttg gaatatctac tgtatcacca agagccgctt ggacaaagtt
    11101 gaggttgaga ttgtagaaga tagtcgttcc ttcacgttca aacttgtcgc tagcttctac
    11161 agaaactact acatacaagt caccataagg tccaccgtta aagcctgctt caccttgacc
    11221 agcgaggcga atttgttgac ctgtttccac accagcaggg attttcacat gtacgctatg
    11281 agcttgtttc tcatgacctg ttccatgaca ggttgtacat ggatatttga tttcttttcc
    11341 tcgaccgtga cagacatcac aggttacttg gcgacgcatc ataccaagag gagtctgcgt
    11401 atcgacgtta atgacaccag cgccatgaca gcgtccacaa gtgactggac ttgtccctgg
    11461 cttagcacca gagccattac atgtacgaca gccagcttca cgatgatact taacttcctt
    11521 ctcagttccg aagatagctt cttcaaaggt caaattgaca cgatactgga gatcatctcc
    11581 ttggcgagga gcgtttggat tgcgcgaaga accgcctccg ccgaagaaac ttgagaaaat
    11641 atcctcaaaa ccaccgaaac cacctgcccc attgaaaccg ccgaaaccac cagctccacc
    11701 aaaaccacca ttggcgcctg cagcaccata ctggtcatag gcagcacgtt tttggtcgtc
    11761 actcaaagtc tcataggctt cttgaacttc cttgtacttg tcctcagcac caggctcctt
    11821 gttgatatct gggtgatatt ttttggaaag cttacgataa gcctttttga tttcgtctgc
    11881 cgaagcgttt ttggataccc ccagacgatc ataaaattca gtattgttca taattcaaga
    11941 tacaaagggc gtcaaacgga cacagccatc taggagtttt gacgacggac gcttacgtcc
    12001 tagaaaaaac tatcttttgg gcacagtccg tagcccgtgt tcagttacga acacccttat
    12061 tcctttctat tgatgttctg aatcgaacca ccatttaggt cgggttcaat tcctttcttt
    12121 tatatttagt aagaaattct gaaacccgtg ttcagttacg aacactcttt gttcccttct
    12181 attgatgaga caaaacccaa acaaatgaga ttccgtccct ttttatcgaa aaacagaggc
    12241 tgggttgcaa cctctgcaaa taagtaaatt aactttattt tgattttaaa tatcctataa
    12301 gtacagaaat atttatatat ttttgtctct tcttagtatt tgcctatata attccaattg
    12361 atcataagaa atacaactac tgtccaaatc ataccaatta tgaacggtat tacaaattta
    12421 tatgtttgta tttctaatga tatcatagct ggtaccatcg ccgatacaaa tgcagacaac
    12481 aatactgagg taacaaaatt ccttaccgct tcatcatttt tgatacaact atcatgttca
    12541 gctaatataa tttctctata tctagctaaa tattctattg gaatattatc aataaaatct
    12601 tttgatgttc tatagttatc agcttgatta caaaaatcat tataaacttt tcgtgtattt
    12661 tttagatact cttcatccaa tacactcatc ttacttttcc gtaaactctc cgtctacgac
    12721 gtcatcgcct gcgttccctg ttgcttgtgc gccttctgct ccttcttgag cttgttgcgc
    12781 tgctgcggct tgttcgtaga gtttaacagc aagtccttga gctttttcgt tcaatgcttc
    12841 aagttttgtt ttcatgtcgt ccaagttgtt gtcttcttga gctttcttaa ggtcatcaag
    12901 ggcagcttgg gcagcgtcac gttctgcgtc gaagccttta ccttcagttt ccttgattgt
    12961 cttttcagtc gcaaagattg cttggtctac ttcattacga aggtctactt cttctttacg
    13021 tttcttatcg gcttcagcgt ttgcttctgc atctttcatc atgcggtcga tttcttcgtc
    13081 agtcaaacct gagttcgatt ggatgacaat agtttgttct ttttgagttc caaggtcttt
    13141 ggccttaaca gacacgatac cgttcttgtc gatgtcaaat gttacttcga tttgaggaat
    13201 tccacgaggt gcagctggga tatcagtcaa ttggaagcgt ccaagagtct tgttatctgc
    13261 tgccattggg cgttcacctt gaagaacgtg gatatcaacg gctggttggt tgtctgctgc
    13321 tgttgagaag acttgtgatt tagatgttgg gattgtagtg ttgcgatcga taagttttgt
    13381 aaatactcca cccattgttt cgataccaag tgacaatggc gttacatcaa gaaggacaac
    13441 gtccttgaca tcaccagtaa tcacaccacc ttggatagcc gcacccatag caactacttc
    13501 atcagggttt actgatttgt ttggttcttt accagtttca gctttaacag cttcaacaac
    13561 ggcagggata cgagttgaac caccaacaag gataacttcg tcgatttctg acaagctcaa
    13621 acctgcatct gaaagggctt gacgaactgg aacttttgta cgttcaacaa ggtcacgagt
    13681 caaatcgtca aatttcgcac gagtcaaagt catttccaag tgaagaggtc cagcctcacc
    13741 tgcagtgata aatggcaagc tgatttgtgt tgaagttaca ccagaaaggt ctttcttcgc
    13801 tttttcagcc gcatctttca aacgttgcat tgccatcttg tcagtagaca agtcgatacc
    13861 gttttctttc ttgaattctg ctaccaagtg gtcaatgatt ttttggtcaa agtcgtcacc
    13921 accaagtttg ttgtcccctg cagttgacaa tacgtcgaag acaccgtcac ccaattcaag
    13981 gatagagacg tcgaatgtac caccaccaag gtcaaatacc aagatttttt cttctttgtc
    14041 agtcttgtcc aaaccataag caagagctgc tgcagttggt tcgttaacaa tacgttctac
    14101 ttcaagacca gcaattttac cagcgtcttt tgttgcttga cgttgagcgt cgttgaagta
    14161 agccggaact gtgataacag ctttggttac tttctcacca aggtagtctt cagcgtagcc
    14221 tttcaagtat tgaaggatca tagctgagat ttcttgtgga gtgtattctt ttccatttgc
    14281 agaaactttt tcagaagttc ccatcttaga tttgatagag ataactgtat ctgggtttgt
    14341 aactgcttga cgttttgcag catcaccaac gatgatttct ccgtttttga atgagactac
    14401 agatggagtt gtgcggtttc cttctgggtt tgcgatgatt ttgctttcag ttccttcaag
    14461 aactgcaact gctgagtttg ttgtacctaa gtcaataccg ataattttag acatgtgttt
    14521 ttctccttgt tagttaattt tctatttttc tttttgatac tcttcttaag tggcgacgcc
    14581 gtcagagctt gacttcgtca gtctctttta ctaaactttg agcctaaggt ctcaaaattt
    14641 gcgcaaatag cgccacggcg aagagtatcg tctttggttc gcttcgctca ctattacaaa
    14701 gcaaaacata ttttatcttt cttcatagtt tattgtcgtt tcggacaagt tttctattat
    14761 gtaaattgcg acacgaggag tcgaaatcga ttttatttcg acgacgagtt agtaaggaag
    14821 ctaggcaaac gccatagcaa ttgccgtttt ctgacgagtt gctttagcta ccgtcagaat
    14881 tgccgaatcg aacacgccct aagctctgag taaaaaagat agattttctt gtgttcatcg
    14941 aacacttcgt caatctccta tttttactgc gagcttttta cgggctttgt atcttagtta
    15001 tacaccacta ccattgctgg gcgtaggatg cggtcatgga gtttgtagcc tttttgaaag
    15061 acttgggcga tggtatctac tgggtgttca tcgtctgctg ggagagtttg gatggccata
    15121 tggtagttat ggtcaaattc gccatctgct gcgatttctt caattccttc ttctttcaaa
    15181 gcgtgaatca agctttcttg caccatcccc aagcccttct tcacatcatc tgtcaaacct
    15241 tcaactgcaa gtgcacgctc aaggttgtca agagatggta agattgcttt tgccaagtcc
    15301 tggctacgat aacgttgcaa gttttgacgt tcttcattgg cacggcgttg gatattttgc
    15361 atttctgcat gagcgcgaag atatttgttt tcgaactcat ctgcacgttc atttgccaag
    15421 tccaactcag acttttcagg agttgtttct tcagctgttt ccacaacttc ctcttcttga
    15481 acttcttcta cttcttcatt ttttatatct tgggccatgt tcgcctcctt taatgatttc
    15541 aatcttaatg tacttcgtaa tgattactgc tgaggtagcg gtaaaaatct gtcaacttca
    15601 tggtcaaaac acggttgacc acattgattt gattgattag ctgttggtaa tccagattaa
    15661 ctggaccgat aatggctaga attccaactc cccgataagg aatgaggaac ttactactaa
    15721 tcaccgctag gtcagctaaa caggactctt gaccgtctgc aacacgaaca ttttgcatct
    15781 gatcctcacg caacccctca cgaatctcca aggccacctt ttgcggttgg tcaaagaact
    15841 gataggctgc tagattggca aaattcaaga gattgacctt gcccgccatc acaatgtttt
    15901 cgttgaacat ttccttaaag atgtgttcaa agagatcgat gacattatcc gttgttgtaa
    15961 agtaacgctg gataatctgc ggaatctccg tccgaatctt gtagtgaata tctaaaacgg
    16021 tgtgaccgag gaaacgttcc tgaatgatgc tcttcagttt cagcaaatcc tcctgcaaga
    16081 agttccttgg aatcagaaac tgactagtaa ccgttcgcga ctcgtctagg gtaaataccg
    16141 ccaaggctgt atgttgcccc aaaacaacga tatcaaaggc tgtcaaacgt tgcctgctcg
    16201 gctcaacatc cagtgccact accgtacagc cactcaggtc tgttagcaag ttagcagcct
    16261 cttgcagaat atcctccaat ttgaagaatt cctgatcaaa ggctttgaca atctcatata
    16321 cctcattttc agccagtcgg tcaaaatcca gtgagtgttt cacatagtac tgaaaaccag
    16381 caacacttgg catccgaccg cttgaagtat gagccttctc aagcaaccct tgtttttcta
    16441 gttccgccat gtcattacga atggttgcac tgctagagtt aatagactct tgcaaggctt
    16501 ttgatccgac aggttcgtgc gttttggtaa agatgtcaat aatcagattt aaaatatcct
    16561 gctgacgctc tgtaaccatc tcatcactcc tctctgtctg atcaattagc actcaatgca
    16621 tccaagtgct aacttatgat tctattatac acccttagaa gagaatgtca agacaaaaac
    16681 tcaaaaaatt agcactcttt tataaagagt gctaaaaatc aatctgaaga cctagaacct
    16741 tgctattcat ctactcggta tttccttttt agtctgaaat aaccatgaat tagataagaa
    16801 atcagaaaag catataaaac aaaaataaga aagaaagaca tagaaagatc ctctctaaac
    16861 aaactcatac aaacaacaag accacctaag aaagtagcta tacaggaacg aagtctagat
    16921 cgcataactt cccatttgag ccccttactc atatagactt gatcctctgg aagtaaatca
    16981 gaagacgatt tacgaagaat cgaacaagaa cctgctccta tcaattccca acctctatct
    17041 ctaaaatctt gcagttcacg cttatgctct ttaagaaatc tagaatcata gatacgatag
    17101 ataacatcgt ccggttgacc ttgctcaaaa tagaacaaac caaaacgact agttctatac
    17161 ctccaacctt tcaaatgcat ctcatgtaaa tattcttctt tcttgtccaa atcaacaatg
    17221 gtgaaaatcc gaaattctac tctgctattc attgtcttac cccaaaatta gaaaacatgc
    17281 ctagcgttat ttattagata attctttcca cttttgactc aatctccaaa aaatataaga
    17341 aatctgaatc gcaaaaacta tcaataaaac ccaatctatt atgaaaatca aaaacacttc
    17401 ccaactgaaa gaactacctc cagtgacaaa ctttgagaaa aacggtagta gagctaaaaa
    17461 gagaagcaaa ataggaagca tccgcattgt taaaatcctt ttggcataaa aaaatcttta
    17521 tttaaacgaa aatattatgg caaaatttac gccagttttt gaacggctga tgtagatatt
    17581 ttatactttc aaaatgttta aatgtgatta tttatttttg aaaaatagct caccagcccg
    17641 actgaaagtg cttatagaat gataataagt cgcctgccga aaacagcgaa aaatagcagt
    17701 gttatgcgga gataatctga cgcgatgcga aagtatattg catacttatt ttcaacaatt
    17761 tagcagagta tttttataag tgtgatataa tagaagtata atttgttctg atagtttatt
    17821 ttatggagaa gtagattttt agaatgcgga ggattcaata tggttgagtt tataaagtct
    17881 aagaaagaaa tgagtgagga ggatattaaa gcaaatttca tcactcctgc tattgtatcc
    17941 aaaggatgga aaaatggtga gcatatcgct tacgaagaat acttcactga tggtcgaatt
    18001 gaagttagag gagataaggc tcgtcgtaaa gaaggaaaaa aatcagacta ttcactgtat
    18061 taccaatttg gaactcgaat tgcaattgtt gaggcaaagg ataataaaca cagcgttcga
    18121 gcaggattac aacaagctat tgaatatgga gagattttag atgttccatt tgtttattct
    18181 tcgaatggtg atggctttat tgaacacgac cgtatcacga gagaagaacg tgagctggag
    18241 ttagacgaat tccctactcg tgaagaatta ttttctcgta tgacgaagga aaaaggattg
    18301 acgtacgaaa ttacagaagc tatctcaact ccatactata cagacgcctt ctcaatgaaa
    18361 acgccacgct attatcagca aatagctatc aaccgtacta ttgaaacagt tgccagagga
    18421 caaaaacgag taatgtttgt gatggcaaca ggaacgggga aaacgttcat ggcttttcaa
    18481 attattcatc gccttcgaaa agctggtttg gctaaacgag ttttattctt agcggataga
    18541 aacatcttag tagaccaaac gatggctgaa gactttaagc cattcgaaaa ggtaatgacg
    18601 aaaattacac caaaactttt gactgctcct gaaaaattaa attcttttga aatttatcta
    18661 gggctttatc agcaactaac tggtgaagat ggaactgaaa cacattatca aaaatttgat
    18721 aaagacttct ttgatttaat cgtaattgat gaagcgcacc gtggttcagc taaggaaaac
    18781 agtaactggc gtaagataat tgattatttc agttctgcga cacagattgg gatgaccgct
    18841 actcctaaag aaaccaagaa tgcttccaat acggaatact ttggtgagcc aatctatact
    18901 tatagtttaa aacagggaat cgaggatggt tttttggctc catatcgtgt tatgagggtt
    18961 aatttagatg tggatgtgga tggttatcgt ccagaaactg gaaaagttga tactaacgga
    19021 caattaatag aagataggta ctacggcagg aaagattttg ataaaaccat tgtcattgat
    19081 gatagaacgc aaagagttgc caagtttgtt tctgattata tgaagcaaaa caatgcacga
    19141 tttgataaaa caattgtttt ttgtgttgat attgaccatg ccgagcgaat gcgtgctgca
    19201 cttgtaaaag agaatctaga cttagtccaa gaagactatc gttatgtcat gcaagtaact
    19261 ggtgacaacg ctgaaggaaa agctcaactg gataacttta tggatgtcaa ttctaatttt
    19321 cccgctatgg taacaacgtc taaattatta acgacaggag ttaatgctaa aacatgtcgt
    19381 ttgattgttt tagactctaa tatccaatcc atgactgaat ttaaacaaat tattggtcgt
    19441 ggcacacgtc tttatcctca aaaggggaaa gaatttttta cgattattga ttttcgaaat
    19501 gttaccaatt tgtttgctga ccctgatttt gatggtgagc cagtgaaggt gctggaaaca
    19561 ggtgcgaaaa cagtcagtgg ttccacgccc ggtttcgtag atgaggaagg tgacccagta
    19621 gaaaaatata tcgttacaga caagcaggtt accattctta attctactgt tcaagtattg
    19681 gatgaaaacg ggaaactgat taccgaaagc ctgaccgact acactcggaa gaatatctta
    19741 ggtagctacg ccactttgaa cgattttatc acagtttggc atacggcgga taaaaagaag
    19801 cttatcttag acgaacttta taaaaaagga gtttatctag atgctattcg agagtcggag
    19861 ggaatatcag aacaagaaat cgatgatttt gatttactcc taaaacttgc ctatggtcaa
    19921 aaagaattaa ccaaaacgga acgtatcaat aagctcaaac aaagcggata tttatataaa
    19981 tatagtgagg aagcgcgtgc tgttttggaa attttactga acaaatacat ggataaaggt
    20041 attggagaac tcgaaagcat tgaaacatta aaacttccag aatttcagat atatggtgga
    20101 accttcaaaa tcatcaatac ttattttgga gataaaaaac gatatttaca agcaattaaa
    20161 gaattggagc aagagctatt tacagtagct taatgaaagg aaagtatgtc aattacatca
    20221 tttgtaaaaa gaattcaaga tatcactcga aacgatgctg gtgttaatgg tgatgctcaa
    20281 cgtattgagc aaatgtcttg gttattattc ttaaaaattt atgatagccg tgaaatggtt
    20341 tgggaattag aagaagacga gtatgagtca attatcccag aggaattaaa atggcgaaat
    20401 tgggctcatg ctcaaaatgg ggaacgggta ttgacaggcg atgaattact tgattttgtc
    20461 aataacaagt tattcaaaga gttgaaagag cttgaaataa cttcaattat gcctattcga
    20521 aaaacgattg ttaaatcagc ttttgaagat gcgaacaact atatgaaaaa tggcgtcttg
    20581 ttacgccaag tcatcaatgt tattgatgaa gttgatttca atagccctga agatcgtcat
    20641 tcgtttaatg atatttacga aaaaattctt aaagatattc aaaatgctgg gaactcagga
    20701 gaattttata cgccacgtgc agcgactgat tttattgccg aagttcttaa cccaaaactt
    20761 ggagaatcaa tggcagacct tgcttgcgga acaggaggct tcttgacttc gactctgaac
    20821 cgtttaagta gtcaacgtaa aactagtgaa gataccaaaa aatataatac agctgttttt
    20881 ggtattgaaa agaaagcatt tcctcatctt ttagcagtta caaatctgtt tcttcacgaa
    20941 attgatgacc ctaaaattgt tcatggaaat actttggaga aaaatgttcg tgaatatacg
    21001 gatgatgaaa aatttgacat tattatgatg aatccacctt ttggagggtc agaattagaa
    21061 acaataaaaa ataactttcc agcagaatta cggagttctg aaacagctga tttatttatg
    21121 gctgtcatta tgtatcgttt gaaagaaaat ggtcgtgttg gagttatttt acctgatggt
    21181 tttctatttg gtgaaggtgt aaaaactcgc ttgaaacaaa aactggtaga tgagttcaac
    21241 ttgcatacga ttattaggtt gcctcatagt gtctttgcac cgtatacagg aatccatacg
    21301 aacattcttt tctttgataa aacaaagaaa acagaagaaa cttggtttta tcgtttagat
    21361 atgccagatg gttataaaaa tttctcgaaa actaagccga tgaagtcaga acacttcaat
    21421 cctgttcgtg actggtggga aaatcgtgaa gagattctgg aaggtaagtt ctacaaatct
    21481 aaatcattta cacctagtga attggctgag ttgaattata atttagacca gtgtggcttt
    21541 ccaaaagagg aagaggaaat cttaaatccc tttgagttga ttcagaatta tcaagcggaa
    21601 agagcaactt taaatcataa gattgataat gtattagctg atattttgca gttgttggag
    21661 gacaaataat gacaccagaa caacttaaag caagtattct ccaaagagcg atggaaggga
    21721 aattagtgcc gcaaaatccc aatgacgaac ctgcaagtga attattaaag agaattaaag
    21781 ctgaaaaaga aaaacttatc agtgaaggaa aaatcaaacg agataaaaag gaaactgaga
    21841 tatttcgtgg tgatgatggg aaacattatg ggaagtttgc tgatggaagt actcaagaaa
    21901 ttgatgttcc ttatgatatt cctgatactt gggagtgggt gaggataaaa tcaatttatt
    21961 ggaattttgg gcaaaataag ccagagaaat cctttaggta tatagatacg tctagtattg
    22021 atagaaaaaa gaacataatc aactacaaaa atctacaata tctttcacct gaacaagcgc
    22081 cttcccgtgc tagaaaatta gtttcgcaga atagtgtctt attttcaaca gttagaccat
    22141 atctaaaaaa tattgctgta gttagagaac ttaaagagta tttgatagct agtacagcat
    22201 ttatt
//
LOCUS       DAVKFH010000025        21612 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKFH010000025 DAVKFH010000000
VERSION     DAVKFH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.025"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..160
                     /locus_tag="VW006_001157"
                     /pseudo
     CDS             1..160
                     /locus_tag="VW006_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            555..941
                     /locus_tag="VW006_001158"
     CDS             555..941
                     /locus_tag="VW006_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase"
                     /protein_id="HEU9380021.1"
                     /translation="MFAWFLFYEVGSKKDYVSYINPGLWGIAFIVCVLVAFAIFPKME
                     KNFPKTFLYFTKYGKDSLGIYILHAPICSMIRILMLKVGINSVFLHVVVGIVLGWYLS
                     ILATYILKKIPFLNIVLLPQKYIKLK"
     gene            1119..3101
                     /locus_tag="VW006_001159"
     CDS             1119..3101
                     /locus_tag="VW006_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9380022.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKVLDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            3402..8705
                     /locus_tag="VW006_001160"
     CDS             3402..8705
                     /locus_tag="VW006_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9380023.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDERDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KQALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEITVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASVTVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(8872..11031)
                     /locus_tag="VW006_001161"
     CDS             complement(8872..11031)
                     /locus_tag="VW006_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9380024.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(11028..11624)
                     /gene="recU"
                     /locus_tag="VW006_001162"
     CDS             complement(11028..11624)
                     /gene="recU"
                     /locus_tag="VW006_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9380025.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            11690..12217
                     /locus_tag="VW006_001163"
     CDS             11690..12217
                     /locus_tag="VW006_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9380026.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            12287..12616
                     /gene="gpsB"
                     /locus_tag="VW006_001164"
     CDS             12287..12616
                     /gene="gpsB"
                     /locus_tag="VW006_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9380027.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            12637..13017
                     /gene="rnpB"
                     /locus_tag="VW006_001165"
     ncRNA           12637..13017
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW006_001165"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13102..14259
                     /locus_tag="VW006_001166"
     CDS             13102..14259
                     /locus_tag="VW006_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9380028.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREMRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            14272..15666
                     /gene="mapZ"
                     /locus_tag="VW006_001167"
     CDS             14272..15666
                     /gene="mapZ"
                     /locus_tag="VW006_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9380029.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            15742..17166
                     /gene="gndA"
                     /locus_tag="VW006_001168"
     CDS             15742..17166
                     /gene="gndA"
                     /locus_tag="VW006_001168"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9380030.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIKALLPYLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            17178..17867
                     /locus_tag="VW006_001169"
     CDS             17178..17867
                     /locus_tag="VW006_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380031.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGIGYTMQE"
     gene            17965..18963
                     /gene="cbpJ"
                     /locus_tag="VW006_001170"
     CDS             17965..18963
                     /gene="cbpJ"
                     /locus_tag="VW006_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9380032.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(19081..20313)
                     /locus_tag="VW006_001171"
     CDS             complement(19081..20313)
                     /locus_tag="VW006_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9380033.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20356..21318)
                     /locus_tag="VW006_001172"
     CDS             complement(20356..21318)
                     /locus_tag="VW006_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9380034.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         6706 a         3853 c         4804 g         6249 t
ORIGIN      
        1 tccacgtaaa tccagcaaac tcaagccgct aatagctgaa gataaagcct ataaccatgc
       61 actgtctaag gatagaagca aggttgagaa catccttgcc aaagtaaaaa cgtttaaaat
      121 gttttcaaca acctatcgaa atcatcgtaa acgcttcgga tattttaggc atactatata
      181 ttttttattc aattattcac ttttgtattc aaaaggttgc gggtgcctct gtccgagtac
      241 ccactatgat atttaaccta ttgaatatct ataaatttcc tctgggagtt tcatggtatc
      301 tatatacgtt atggtcgata ttgatagttt atggtttact atctgttgtt ttcaaaaatc
      361 gtaagtccct tttgttagtt agtgtttttg cctatatttt cacactattt attcaaactg
      421 atatttttat tgtgcaacgg acgttggttt gggggatttg tttctttctt ggcagtgtat
      481 tgagtgaaat tcactttgat aaaattaatt tgaaaaaatt tctttttttc tttgtgttat
      541 ttgattttat ttatatgttc gcttggttct tgttttatga agtagggtct aagaaggatt
      601 atgtaagcta tattaaccca ggtttgtggg ggattgcttt tattgtctgt gtattagttg
      661 cttttgcgat ttttcctaaa atggagaaaa attttcctaa aactttccta tatttcacta
      721 aatacgggaa agatagttta gggatctata ttcttcatgc accaatttgt agcatgattc
      781 ggattctaat gttgaaagtg ggaataaact cagtttttct tcacgttgtt gttgggattg
      841 tgctaggctg gtatttatcc atactagcaa cttatatatt gaaaaaaatt ccatttttga
      901 atattgtttt attaccacaa aagtatatta aattaaaata aattatttta gatttataat
      961 gtggctcttt gtcaactaac atctggagag gacaatcact gtcttctctt tttttgtctt
     1021 ttcagaatat accaaattaa cacaaaaatt ctgaaaattc tgttgacatc tttctgaaaa
     1081 gagtttataa tggagagaaa gttttaaagg agaaaatgat gaaaagttca aaactatttg
     1141 cccttgcggg cgtgacatta ttggcggcga ctactttagc tgcatgctct ggatcaggtt
     1201 caagcactaa aggtgagaag acattctcat acatttatga gacagaccct gataacctca
     1261 actatttgac aactgctaag gctgcgacag caaatattac cagtaacgtg gttgatggtt
     1321 tgctagaaaa tgatcgctac gggaactttg tgccgtctat ggctgaggat tggtctgtat
     1381 ccaaggatgg attgacttac acttatacta tccgtaagga tgcaaaatgg tatacttctg
     1441 aaggggaaga atacgcggca gtcaaagctc aagactttgt aacaggacta aaatatgctg
     1501 ctgataaaaa atcagatgct ctttaccttg ttcaagaatc aatcaaaggg ttggatgcct
     1561 atgtaaaagg ggaaatcaaa gatttctcac aagtaggaat taaggttctg gatgaacaga
     1621 cagttcagta cactttgaac aaaccagaaa gcttctggaa ttctaagaca accatgggtg
     1681 tgcttgcgcc agttaatgaa gagtttttga attcaaaagg ggatgatttt gccaaagcta
     1741 cggatccaag tagtctcttg tataacggtc cttatttgtt gaaatccatt gtgaccaaat
     1801 cttctgttga atttgcgaaa aatccgaact actgggataa ggacaatgtg catattgaca
     1861 aagttaaatt gtcattctgg gatggtcaag ataccagcaa acctgcagaa aactttaaag
     1921 atggtagcct tacagcagct cgtctctatc caacaagtgc aagtttcgca gagcttgaga
     1981 agagtatgaa ggacaatatt gtctatactc aacaagactc tattacgtat ctagttggta
     2041 caaatattga ccgtcagtcc tataaataca catctaagac cagcgacgaa caaaaggcat
     2101 cgactaaaaa ggctctctta aacaaggatt tccgtcaggc tattgccttt ggttttgatc
     2161 gtacagccta tgcctctcag ttgaatggac aaactggagc aagtaaaatc ttgcgtaatc
     2221 tctttgtgcc accaacattt gttcaagcag atggtaaaaa ctttggcgat atggtcaaag
     2281 agaaattggt cacttatggg gatgaatgga aggatgttaa tcttgcagat tctcaggatg
     2341 gtctttacaa tccagaaaaa gccaaggctg aatttgctaa agctaaatca gccttacaag
     2401 cagaaggtgt gacattccca attcatttgg atatgccagt tgaccagaca gcaactacaa
     2461 aagttcagcg cgtccaatct atgaaacaat ccttggaagc aactttagga gctgataatg
     2521 tcattattga tatccaacaa ctacaaaaag acgaagtaaa caatattaca tattttgctg
     2581 aaaatgctgc tggcgaagac tgggatttat cagataatgt cggttggggt ccagactttg
     2641 ccgatccatc aacttacctt gatatcatca aaccatctgt aggagaaagt actaaaacat
     2701 atttagggtt tgactcaggg gaagataatg tagctgctaa aaaagtaggt ctatatgact
     2761 acgaaaaatt ggttactgag gctggtgatg aggctacaga tgttgctaaa cgctatgata
     2821 aatacgctgc agcccaagct tggttgacag atagtgcttt gattattcca actacatctc
     2881 gtacagggcg cccaatcttg tctaagatgg taccatttac aataccattt gcattgtcag
     2941 gaaataaagg tacaagtgaa ccaatcttat ataaatactt ggaacttcaa gacaaggcag
     3001 tcactgtaga tgaataccaa aaagctcagg aaaaatggat gaaagaaaaa gaagagtcta
     3061 ataaaaaggc tcaagaagat ctcgcaaaac atgtgaaata actgttgcaa aatataagaa
     3121 aggatttagt atttctcttg aatgctgaat ccttttttac atttgtaaag aaagattcta
     3181 aatgtactga cccccaaaag ttggagcctc tttttgtcag aatagagaaa atttttgtta
     3241 attttacttg tttcctattg ctttatcagt tattatttgt tatattaaaa gtataattat
     3301 tttttattta tcagggttaa gcattgcact ttcagaggaa ggagtatttt ttaaaaagga
     3361 aatgtaaacg cttgcccaaa aatgaaagga tttagaagtt tatgaataaa ggattatttg
     3421 aaaaacgttg taaatatagt attcggaaat tttcattagg tgttgcttct gttatgattg
     3481 gagctgcatt ctttgggaca agtccggttc ttgcagatag cgtgcagtct ggttctacgg
     3541 cgaatttacc agctgattta gctactgctc ttgcaacagc aaaagagaat gatgagcgtg
     3601 attttgaagc gcctaaggtg ggagaagacc aaggttctcc agaagttaca gatggaccta
     3661 agacagaaga agaactatta gcacttgaaa aagaaaaacc ggctgaagaa aaaccaaaag
     3721 aggataaacc tgcagctgct aaacctgaaa cacctaagac ggtaacccct gaatggcaaa
     3781 cggtagagaa aaaagaacaa caaggaacag tcactatccg agaagaaaaa ggtgtccgct
     3841 acaaccaact atcctcaact gctcaaaatg ataacgcagg caaacaagcc ctgtttgaaa
     3901 agaagggctt gaccgttgat gccaatggaa atgcaactgt tgatttaacc ttcaaagaag
     3961 attctgaaaa gggcaaatca cgctttggtg tcttcttgaa atttaaagat accaataata
     4021 atgtttttgt cggttatgac aaggatggct ggttctggga gtataaatct ccaacaacta
     4081 gcacttggta tagaggtagt cgtgttgctg ctcctgaaac aggatcaaca aaccgtctct
     4141 ctatcactct caagtcagac ggtcagctaa atgccagcaa taacgatgtc aatctctttg
     4201 acacagtgac tctaccagct gcggtcaatg accatcttaa aaatgagaag aagattcttc
     4261 tcaaggcggg ctcttatgac gatgagcgaa cagttgttag cgttaaaacg gataaccaag
     4321 agggggtaaa aacagaggat acccctgctg aaaaagaaac aggtcctgaa gttgatgata
     4381 gcaaggtgac ttatgacacg attcagtcta aggtcctcaa agcagtgatt gaccaagcct
     4441 tccctcgtgt caaggaatac agcttgaatg gacatacttt gccaggacag gtgcaacagt
     4501 tcaaccaagt ctttatcaat aaccaccgaa tcacccctga agtcacttat aagaaaatca
     4561 atgagacaac agcagagtac ttgatgaaga ttcgcgatga tgctcactta atcaatgcgg
     4621 aaataacagt acgcttgcaa gttgtggaca atcaattgca ctttgatgtg accaagattg
     4681 tcaaccacaa tcaagtcact ccaggtcaaa agattgatga cgaaagaaaa ctactttctt
     4741 ctattagttt cctcggcaat gctttagtct ctgtttctag tgatcaaact ggtgctaagt
     4801 ttgatggggc aaccatgtca aacaatacgc atgtcagcgg agatgatcat atcgatgtaa
     4861 ccaatccaat gaaggatttg gctaagggtt acatgtatgg atttgtttct acagataagc
     4921 ttgctgctgg tgtttggagt aactctcaaa atagctatgg tggtggttcg aatgactgga
     4981 ctcgtttgac agcttataaa gaaacagtcg gaaatgccaa ctatgtagga atccatagct
     5041 ctgaatggca atgggaaaaa gcttataagg gcattgtttt cccagaatac acgaaggaac
     5101 ttccaagtgc taaggttgtt atcactgaag atgccaatgc agacaagaaa gttgattggc
     5161 aagatggtgc cattgcttat cgtagcatta tgaacaatcc tcaaggttgg gaaaaagtta
     5221 aggatatcac agcttaccgt atcgcgatga actttggttc tcaagcacaa aacccattcc
     5281 ttatgacctt ggatggtatc aagaaaatca atctccacac agatggtctt gggcaaggtg
     5341 ttctccttaa aggatatggt agcgaaggcc atgactctgg tcacttgaac tatgctgata
     5401 ttggtaagcg tatcggtggt gtcgaagact tcaagaccct aattgagaag gctaagaaat
     5461 atggagctca tctaggtatc cacgttaacg cttcagaaac ttatcctgag tctaaatact
     5521 tcaatgaaaa aattctccgt aagaatccag atggaagcta tagctatggt tggaactggc
     5581 tagatcaagg tatcaacatt gatgctgcct atgacctagc tcatggtcgt ttggcacgtt
     5641 gggaagattt gaagaaaaaa cttggtgacg gtctcgactt tatctatgtg gacgtttggg
     5701 gtaatggtca atcaggtgat aacggtgcct gggctaccca cgttcttgct aaagaaatta
     5761 acaaacaagg ctggcgcttt gcgatcgagt ggggccatgg tggtgagtac gactctacct
     5821 tccatcactg ggcagctgac ttgacctacg gtggctacac caataaaggt atcaacagtg
     5881 ccatcacccg ctttatccgt aaccaccaaa aagatgcttg ggttggggac tacagaagtt
     5941 atggtggtgc agccaactat ccactgctag gtggctacag catgaaagac tttgaaggct
     6001 ggcaaggaag aagtgactac aatggctatg taaccaactt atttgcccat gacgtcatga
     6061 ccaagtattt ccaacacttc actgtaagca aatgggaaaa tggtacaccg gtgactatga
     6121 ccgataacgg tagcacctat aaatggactc cagaaatgcg agtggaattg gtagatgctg
     6181 acaataataa agtagttgta actcgtaagt caaatgatgt caatagtcca caatatcgcg
     6241 aacgtacagt aacgctcaac ggacgtgtca tccaagatgg ttcagcttac ttgactcctt
     6301 ggaactggga tgcaaatggt aagaaacttt ctactgataa ggaaaagatg tactacttca
     6361 atacgcaggc cggtgcaaca acttggaccc ttccaagcga ttgggcaaag agcaaggttt
     6421 acctttacaa gctaactgac caaggtaaga cagaagagca agaactaact gtaaaagatg
     6481 gtaaaattac tctagatctt ctagcaaatc aaccatacgt tctctatcgt tcgaaacaaa
     6541 ccaatcctga aatgtcatgg agcgaaggca tgcacatcta tgaccaagga tttaacagtg
     6601 gtaccttgaa acattggacc atttcaggcg atgcttctaa ggcagaaatt gtcaagtctc
     6661 aaggggcaaa cgatatgctt cgtattcaag gaaacaaaga aaaagttagt ctcactcaga
     6721 aattaactgg cttgaaacca aataccaagt atgccgttta tgtcggtgtc gataaccgta
     6781 gtaatgccaa ggcgagcgtc actgtaaata ctggtgaaaa agaagtgact acttatacca
     6841 ataagtctct cgccctcaac tatgtaaaag cctacgccca caatacacgt cgtaacaatg
     6901 ctacagttga cgatacaagt tacttccaaa acatgtacgc cttctttaca actggatcgg
     6961 acgtatcaaa tgttactctg acattgagtc gtgaagctgg tgatcaagca acttactttg
     7021 atgaaattcg tacctttgaa aacaattcaa gcatgtacgg agacaagcat gatacaggta
     7081 aaggcacctt caagcaagac tttgaaaatg ttgctcaggg tatcttccca tttgtagtgg
     7141 gtggtgtcga aggtgttgaa gataaccgca ctcacttgtc tgaaaaacac aatccatata
     7201 cacaacgtgg ttggaatggt aagaaagtcg atgatgttat cgaaggaaat tggtcactca
     7261 agacaaatgg actagtgagc cgtcgtaact tggtttacca aaccatccca caaaacttcc
     7321 gttttgaagc aggtaagacc taccgtgtaa cctttgaata cgaagcagga tcagacaata
     7381 cctatgcttt tgtagtcggt aagggagaat tccagtcagg tcgtcgtggt actcaagcaa
     7441 gcaacttgga aatgcatgaa ttgccaaata cttggacaga ttctaagaaa gccaagaagg
     7501 caaccttcct tgtgacaggt gcagaaacag gcgatacttg ggtaggtatc tactcaactg
     7561 gaaatgcaag taatactcgt ggtgattctg gtggaaatgc caacttccgt ggttataacg
     7621 acttcatgat ggataatctt caaatcgaag aaattaccct aacaggtaag atgttgacag
     7681 aaaatgctct gaagaactac ttgccaacgg ttgccatgac taactacacc aaagagtcta
     7741 tggatgcttt gaaagaggcg gtctttaacc tcagtcaggc cgatgatgat atcagtgtgg
     7801 aagaagcgcg tgcagagatt gccaagattg aagctttgaa gaatgctttg gttcagaaga
     7861 aaacggcttt ggtagcagat gactttgcaa gtcttacagc tcctgctcag gctcaagaag
     7921 gtcttgcaaa tgcctttgat ggcaatgtgt ctagtctatg gcatacatct tggaatggtg
     7981 gagatgtagg caagcctgca accatggtct tgaaagaacc aactgaaatc actggacttc
     8041 gttatgttcc acgtggatca ggttcaaatg gtaacttgcg tgatgtgaaa cttgttgtga
     8101 cagatgagtc tggcaaggag cataccttta ctgcaactga ttggccagat aacaataagc
     8161 caaaagacat tgattttggt aagacaatta aggctaagaa aattgtcctt acaggtacta
     8221 agacttacgg agatggtggc gataaatacc aatctgcagc ggaacttatc tttactcgtc
     8281 cacaggtagc agaaacacct cttgacttgt caggctatga agcagctttg gctaaggctc
     8341 agaaattaac agacaaagac aatcaagagg aagtagctag cgttcaggca agcatgaaat
     8401 atgcgacgga taaccatctc ttgacggaaa ggatggtgga atactttgca gattatctca
     8461 accaattaaa agattctgct acgaaaccag atgctccaac tgtagagaaa cctgagttta
     8521 aacttagctc tttagtttcc aatcaaggta agacgccaga ttataagcaa gaaatagata
     8581 gaccagaaac acctgaacaa atcttgccag caacaggtga gagtcaatct gacacagccc
     8641 tcttcctagc aagtgttagt ctagccctat ctgctctctt tgtagtaaaa acgaagaaag
     8701 actagtattt agtaaaacct cttaacaaga ttacggaagc agtctctatc ttttccaatg
     8761 aggtttatag tacagaaaaa gcctgagaag atgtcttctc aggcttttgt taagcacata
     8821 aatacaatag tgctatgaca aaatcaccca gaaaaatctg ggtgataaat gttatggttg
     8881 tgctggttga ggattctgat tttgttgatc aggggttgta tttgattgtt gcgtattatt
     8941 gttaggattg gtagtcgtac tattatttgt gcttggagtg gttgagctag actgtgaagt
     9001 tgaactatct gatgatgagc ttgaactttc agttgatggg ggttgttgtg gagcaggtga
     9061 gttccacgta gaacgagcac catttttaaa tacgaattct ccatttctgt agagcccctc
     9121 tggtatattc caatcctctg gattgcttcc ttcagacaga taggtcatca tagagcggta
     9181 aactttggca gcgaccgtaa ggccattgcc tacaagtggt gtcagacggt tagaatagcc
     9241 tgtccataca gccattgaat atttacgcgt atagccagca aatagttcat caggtgctac
     9301 aaattgagag gtcttgatgt ggttttcaat ttcctcgtct gtatagttag aggttcctgt
     9361 tttaccagcc tgagggagcc aagcaagata ggcatttcgt ccagttccat aactcaagac
     9421 tgttttcatc atgtcggtca tcatataggc tgtcgtttcc ttcatggcac gagttccgac
     9481 attagagaac tctttttcac tcccatcact aaagacgact ttatggatat acattggttt
     9541 atagtaagtt ccaccatttg caaaggcagc gtaagcagca gccatctttt cactacttgc
     9601 tccatatttt ttgtctgatt cggttgtgtt acttgaaatg gcatttgagt agtgaatact
     9661 tgggtagtcg attccgagac catttaggaa agtcttggcg cggttgagtc cgaccttgtt
     9721 tagagtttcc acggctggga cgtttcgcga ttgttgcagg gcgtattgca aggtgatgtt
     9781 gccaaagtag cccctatccc agttataaac aggggtattt gtcccagggt agttataggg
     9841 ctcatcgtga acgatagtag cagttgaatc gtagataccg tactccaagg caggagcata
     9901 gtctgtgatc ggtttcatag ttgatcccca gtcgcggttt gtttctactg cttggttaat
     9961 tccgaaggaa acattacttg actgatggcg tgctcctagc tgggcaatga ctttaccgtt
    10021 agaaacatca acaatggtag aagcgacttg caattcatcg tctggatagg caacgtattc
    10081 gtctgtattg taaatatccc acagatgttt ttgagcttct tggtctacat ttgtgtagac
    10141 atccatccca gttgtgagca ggttatagcc tgtttcttct tcaacttgat tgatgacttc
    10201 cttgaggtaa ttatccatgt aagcagggta attacttgct gatttgagac tttgtagtcc
    10261 atcagtaatt ggtgtattga ctgctttctc atactgttca gcagagatgt agccttgatt
    10321 tttcatttca gataagacca agtttcggcg gtcttgggct gcttctggat gtgaataggg
    10381 gtcatattgg tttggtgcct gaggcattcc agccagcaag gctaactgag gtaaacttaa
    10441 attattgagg tctttaccat agtagttttg agctgctgtc tgcattccat agttcccatt
    10501 agacatgtag accttattta tatagtaggt caagatttct tgcttggttg ctttttgttc
    10561 taactgaatc gctaaccaag cttcctgagc cttacgagaa atagtctggt cggaagtcga
    10621 agttgaaaag taagtcaact taatcaactg ttgggtgaga gttgatccac cttggaggga
    10681 attgctttgc agattgcgca agaaagctcc taggatacgg atggtatcaa tccccctgtg
    10741 gtcgaagaag cgatggtctt cgatagaaac gattgcctta accaaatctg tgggaatatc
    10801 attagcttgg gcattgacgc ggcgttcaga acccaagtca gcaatgagtt gatttttatt
    10861 gtcgtagatt ttactagaag ttgttgcaac tagtttactc tcggataggc taggagcctt
    10921 gctaacgtag tagaaaaaaa ctcctccgcc taagacaatg gctgcgataa ccaagcttaa
    10981 gaagctaatg ctcagatact tgattaggcg cagaatcgtt ggtttgttca tcttgtttta
    11041 ccacctaata aatgttcttt gataacattg agataaggaa tttgagggaa ggcaccagcc
    11101 ttgatttcat atccatattc tcgaatatat tcaagtggca ttgatttttg tcccttatct
    11161 tgatgataga agcgaatcaa atcgaatgcc ggcaataagt aggtttcttg ctgagaagaa
    11221 aagtgaagaa ggacaaagca gattccttgt tgggcaagga cttgttccat atgctgaatc
    11281 tgatgtggat gaaaattttt catcggaatc gcacgttttt gttttgtttc cttgacttca
    11341 aagtcgatgt aatatccatt ataaacgcca gaatagtccg tcgttgaagc ttgtcgaaaa
    11401 taggcttcaa caatcttggc acgacttcgt tgtggatagt ccacttgtac gatttgaata
    11461 ggagttggtt tcttatgtat aacagccaag ccctgagaca aatagtagtc gttggtagca
    11521 ttgatcatct tttcaaaaga cattcctcga tttgcgaaat ttttgggttg agaaagagat
    11581 gtttgtcttt tttgtgatga aactttatgt ggatagttga ccataattct ccttattggt
    11641 acaataacat cactttatta taccataatt tgcacagaaa gggttaaaaa tggctacagc
    11701 tttggtttta ggctattctg cttttgattt gggtttattt tctgataagg atcctcgtct
    11761 taaattgatt aaaaaagcga tacgtaaaga tttagaggct atggcagcag atggggtgtc
    11821 ttggcttgtg tttacaggga gtttgggttt tgaatattgg gttttagagg ttgctcagga
    11881 gatgaaaact gaatacggtt tccagttggc caccattttt gcttttgaaa cccatgggga
    11941 aaattggaat gaaggcaatc agatgaaact gagtcgcttt aagcaggtag actttgtcaa
    12001 atatgcctat cctcgctatg aacacaaggg gcagttaaga gattaccagc agtttttgct
    12061 ggaaaacacg actagttcct atctttttta tgatgaagaa aatgaaacaa aactagctta
    12121 tttttaccaa aagatgaaaa atcaagagga ctattttata aagagattaa catttgatga
    12181 attaaatgaa cttgctgaaa atttttccga aaattgaagc tttgaccttg atttttgttt
    12241 gccttttttt atataataat actagcaagc gagaatggag agagacatgg caagtattat
    12301 tttttcagcg aaagatattt ttgaacaaga gtttggacgt gaagtccgtg gctataataa
    12361 agtagaagtt gacgagtttt tagacgatgt catcaaggac tatgaaacct atgctgcctt
    12421 ggtcaagtca cttcgtcagg aaattgcgga tttgaaggaa gaattaactc gtaaaccgaa
    12481 accttcacca gttcaagcag aaccccttga agcggcaatt acaagttcta tgacgaattt
    12541 tgatattttg aaacgcctga atagattgga aaaagaagtt tttggtaaac aaattttaga
    12601 taactcagat ttttaagtag ttatttgaga tgtgcaattt ttggataatc gcgtgaggag
    12661 aattgcttct catgaggaaa gtccatgcta gcacaggctg tgatgcctgt agtgtttgtg
    12721 ctaggcgaaa ccataagcct agggacgaga aatcgttacg gcagttgaaa tggctaagtc
    12781 cttggatagg ccagagtagg cttgaaagtg ccacagtgac ggagtctttc tggaaacaga
    12841 gagagtggaa cgcggtaaac ccctcaagct agcaacccaa attttggtcg gggcatggag
    12901 tacgcggaaa cgaacgtagt attctgactg ctatcagcta gagctgttag tggtagacag
    12961 atgattatcg aaggaagtgg tcctagtcac ttctggaaca aaacatggct tatagaaaat
    13021 tgcatatagg ttggggctga gaaattttct caacctcatt ttttaaagtg gacatataga
    13081 aaggtcttgc aagactgtaa catgaaaaaa gaatttaatt taattgcaac tgtggcagca
    13141 gggcttgagg ctgtcgttgg tcgtgaaatg cgagagttgg gctacgattg tcaggttgaa
    13201 aatggacgtg ttcgttttca aggagacgtg agagctatta tcgaaaccaa cctttggctt
    13261 cgggcagcag atcgtatcaa aattatcgta ggaacgttcc cagctaagac ttttgaagag
    13321 ctatttcagg gagttttcgc tttagattgg gaaaattatt taccacttgg agctcggttc
    13381 ccgatttcaa aagctaaatg tgttaagtcc aaacttcaca atgagcccag tgttcaggct
    13441 atttctaaga aagctgttgt caagaaattg cagaaacact atgctcgccc agaaggggtt
    13501 cctctgatgg agaatggtcc agagtttaag attgaggtct ctattctcaa agatgtggca
    13561 actgtcatga ttgatacgac cgggtctagc ctctttaaac gtggttatcg taccgaaaaa
    13621 ggtggcgctc ctatcaagga aaatatggca gcagccattt tacaactttc taactggtat
    13681 ccagacaagc ctttgattga tccgacctgt ggttcgggga ctttctgtat tgaggcagtt
    13741 atgattgcta gaaagatggc gccaggtctt cgtcgttctt ttgcatttga ggaatggaac
    13801 tggatcagcg atcgcttgat tcaagaagtg cgcacagaag cggctaaaaa agtagaccgt
    13861 gagcttgagc tggatatcat gggctgtgat attgatgctc gcatggtgga aattgctaag
    13921 gccaatgctc aggtagctgg tgttgcagga gacattactt ttaagcagat gcgcgtgcag
    13981 gatttatgtt ccgataaaat caatggagta atcatttcca atccgcctta tggtgaacgt
    14041 ttgtcagatg atgcaggggt gaccaagctc tatgctgaga tggggcaagt atttgcaccg
    14101 ctgaaaactt ggagcaaatt tatcctgact agtgatgaag cttttgaaag caagtatggt
    14161 agccaagcag ataagaagcg taagttatac aacggaacct tgaaagtgga tctatatcaa
    14221 tattttggtc agcgtgtcaa acggcaagag gtaaaataga aagggatact catgagtaaa
    14281 aaaagacgaa atcgtcataa aaaagaaggt caagaaccgc aatttgattt tgatgaagca
    14341 aaagagctaa cagttggtca agctattcgt aaaaatgaag aagtggaagc aggagtcttg
    14401 cctgaggatt ccattttgga caagtatgtt aagcaacaca gagatgaaat tgaggcggat
    14461 aagtttgcga ctcgtcaata caaaaaagag gagttcgttg aaactcagag tctggatgat
    14521 ttaattcaag agatgcgtga ggctgtagag aagtcagaag cttcttcgga ggaagttcca
    14581 tcttctgaag acatcttact acccttgcct ctggacgatg aggagcaagg cttggatcct
    14641 ctattgctag atgatgaaaa tccaacagaa atgactgaag aagtggaaga ggagcaaaac
    14701 ctttctcgtc tggatcaaga ggactcagaa aagaaaagta aaaaaggctt tattttgacc
    14761 gttttggcgc ttgtatcagt aattatttgt gtcagtgctt attatgtcta ccgtcaagtg
    14821 gctcgttcga ctaaggaaat tgaaacttct caatcaacta cagccaatca atcggatgtg
    14881 gatgatttta atacacttta tgacgccttt tacacagata gcaataaaac ggctttgaaa
    14941 aatagccagt ttgataaact gagtcaactc aagactttac ttgataagct ggaaggtagt
    15001 cgtgaacata cgcttgccaa atctaaatat gatagtctag taacgcaaat taaggccatt
    15061 caagatgtca atgcacaatt tgaaaaacca gctattgtgg atggtgtgtt ggataccaat
    15121 gccaaagcca aatcggatgc taaatttaca gatattaaaa ctgggaatac ggagcttgat
    15181 aaagtgctag ataaggctat cagtcttggt aagagccagc aaacaagtac ttctagctca
    15241 agttcaagtc aaactagcag ctcaagctct agtcaagcaa gctcaaatac gactagtgag
    15301 ccaaaaccaa gtagttcaaa tgagactaga agtagtcgca gtgaagtcaa tatgggtctc
    15361 tcgagtgcag gggttgctgt tcaaagaagt gccagtcgtg ttgcctataa tcagtctgct
    15421 attgatgata gtaataactc tgcctgggat tttgcggatg gtgtcttgga acaaattcta
    15481 gcaacttcac gttcacgtgg ctatatcact ggagaccaat atatccttga acgtgtcaat
    15541 atcgttaacg gcaatggtta ttacaacctc tacaagccag atggaaccta tctctttacc
    15601 cttaactgta agacaggcta ctttgtcgga aatggcgctg gtcatgcgga tgacttggat
    15661 tactaagcag tcgttacaaa attctttctt ttcaaaagta aaaatgataa aataaaacaa
    15721 atgaaacaag aggagtgtca aatgacaaaa gctaactttg gtgttgtagg tatggccgta
    15781 atgggtcgta accttgccct taatattgaa tcacgtggtt acacagttgc tatctacaac
    15841 cgtagtaaag aaaaaacgga agatgtgatt gcttgccatc ctgaaaagaa ctttgtacca
    15901 agctatgacg ttgaaagttt tgtaaactca atcgaaaaac ctcgtcgtat catgctgatg
    15961 gttcaagctg gacctggtac agatgctact atcaaagccc ttcttccata ccttgacaag
    16021 ggtgatatct tgattgacgg tggaaatact ttctacaaag ataccatccg tcgtaatgaa
    16081 gaattggcaa actcaggtat caactttatc ggtactgggg tttctggtgg tgaaaaaggt
    16141 gcccttgaag gtccttctat catgcctggt ggacaaaaag aggcctacga attggttgcg
    16201 gatgttcttg aagaaatctc agctaaagca ccagaagatg gcaaaccatg tgtgacttac
    16261 atcggccctg atggagctgg tcactatgtg aaaatggttc acaatggtat tgagtacggt
    16321 gatatgcaat tgatcgcaga aagctatgac ttgatgcaac acttgctagg cctttctgca
    16381 gaggatatgg ctgaaatctt tactgagtgg aacaagggtg aattagacag ctatttgatc
    16441 gaaatcacag ctgatatttt gagccgtaaa gacgatgaag gccaagatgg accaatcgta
    16501 gactacatcc ttgatgctgc aggtaacaag ggaactggta aatggactag ccaatcatct
    16561 cttgaccttg gtgtaccatt gtcactgatt actgagtcag tgtttgcacg ctacatttca
    16621 acttacaaag aagaacgtgt acatgctagc aaggtgcttc caaaaccagc tgccttcaac
    16681 tttgaaggag acaaggctga attgattgaa aagatccgtc aagcccttta cttttcaaaa
    16741 atcatttcat acgcacaagg atttgctcaa ttacgtgtag cctctaaaga aaacaactgg
    16801 aacttgccat ttgcagatat cgcatctatc tggcgtgatg gctgtatcat ccgttctcgt
    16861 ttcttgcaaa agattacaga tgcttacaac cgtgatgcag accttgccaa ccttcttttg
    16921 gatgaatact tcttggatgt tactgctaag tatcaacaag cagtgcgtga tatcgtagct
    16981 cttgcggttc aagctggtgt gccagtgcca actttctcag cagctattac ttactttgat
    17041 agctaccgtt cagctgacct tccagctaac ttgatccaag cacaacgtga ctactttggt
    17101 gctcacactt accaacgtaa agacaaagaa ggaaccttcc actactcttg gtatgacgaa
    17161 aaataagtag gtcagccatg gggaaacgga ttttattact tgagaaagaa cgaaatctag
    17221 ctcatttttt aagtttggaa ctccagaaag agcagtatcg ggttgatctg gtagaggagg
    17281 ggcaaaaagc cctctccatg gctcttcaga cagactatga tttgatgtta ttgaacgtta
    17341 atctgggaga tatgatggct caggattttg cagaaaaatt gagccgaact aaacctgcct
    17401 cagtcatcat gattttagat cattgggaag acttgcaaga agagctggaa gttgttcagc
    17461 gttttgcagt ttcatacatc tataagccag tccttatcga aaatctggta gcgcgtattt
    17521 cggcgatctt ccgaggtcgg gacttcattg atcaacactg cagtctgatg aaagttccaa
    17581 ggacctaccg caatcttagg atagatgttg aacatcacac ggtttatcgt ggtgaagaga
    17641 tgattgctct gacccgccgt gagtatgacc ttttggcgac acttatggga agcaagaaag
    17701 tattgactcg tgagcaattg ttggaaagtg tttggaagta tgaaagtgcg accgagacaa
    17761 atatcgtaga tgtctatatc cgctatctac ggagcaagct tgatgttaaa ggacaaaaaa
    17821 gctacattaa aactgtgcgt ggtattggat ataccatgca agaatagaaa agcagttgca
    17881 gttgtgtaac tgcttttttg aggagttttt atatattgac atgcagtcgg gtctttgcta
    17941 caatcgtttt aggagggtat atagatgaaa attttgaaaa aaactatgca agttggactg
    18001 acagtatttt tctttggttt gctagggacc agtacagtat ttgcagatga ttctgaagga
    18061 tggcagtttg tccaagaaaa cggaagaacc tactacaaaa agggggacct caaagaaacc
    18121 tactggcgag tgattgatgg taagtactat tattttgatt ctctatctgg agagatggtt
    18181 gtcggctggc aatatatccc gtttccatct aaaggtagta caattggtcc ttacccaaat
    18241 ggtatcagat tagaaggttt tccaaagtca gagtggtact acttcgataa aaatggagtg
    18301 ctacaagagt ttgttggttg gaaaacatta gagattaaaa ctaaagacag tgttggaaga
    18361 aagtacgggg aaaaacgtga agattcagaa gataaagaag agaagcgtta ttatacgaac
    18421 tattacttta atcaaaatca ttctttagag acaggttggc tttatgatca gtctaactgg
    18481 tattatctag ctaagacgga aattaatgga gaaaactacc ttggtggtga aagacgtgcg
    18541 gggtggataa acgatgattc gacttggtac tacctagatc caacaactgg tattatgcaa
    18601 acaggttggc aatatctagg taataagtgg tactacctcc gttcctcagg agcaatggcc
    18661 actggctggt atcaggaagg taccacttgg tattatttag accacccaaa tggcgatatg
    18721 aaaacaggtt ggcaaaacct tgggaacaaa tggtactatc tccgttcatc aggagctatg
    18781 gcaactggtt ggtatcaaga tggttcaact tggtactacc taaatgcagg taatggagac
    18841 atgaagacag gttggttcca ggtcaatggc aactggtact atgcttatag ctcaggtgct
    18901 ttggcagtga atacgaccgt agatggctat tctgtcaact ataatggcga atgggttcgg
    18961 taatgacagg gcgcttataa ttatattaga acactcgctt aatcaaagaa gtgactttgt
    19021 tgtacttgac ctgacgattg gggagattaa aagcatgtga aatctacaca tgctttttcg
    19081 ttagttatga gtgttgaact tgactaacca ttgacaaaag ataagagcaa ttaaaataag
    19141 atacaaaaat atcaatgcta actgcaccga aacgcccgat gctatggata gggctaatag
    19201 ggagagcagg gacggggcga gaatgtccac tgtgctcaga gcagagccca ccgtccccat
    19261 cttgtcactg gggatatggg caaagacagc tgcttgtagg cgagggctga gaatgccgat
    19321 aatcgtagaa ctgatgaaac tggtcatgaa aattacgata taggcttgac aaaggatact
    19381 agttattaga gtcaataggg aaatctcaca gaaaaccaag aggtgcgtga aacggatatt
    19441 tttaaataga ttactgctgg tcatattgcc aaggatagct ccacaagaca tgatgatgga
    19501 gagcagggcg aggagttgcc caatgctcag ttgtgctagt gtctgttgga tgaagcggag
    19561 ggcaatcagc cgaggggaca catccaagat ggcaatcata ctggtcgtca gaaacagtag
    19621 tatgacggta tcttttaacc tcaggagcac agccagcgac tgtttaagat gttgaaaata
    19681 tttcttaaaa ctcagtgctg tatgtgacat ttcaattctt ttttcaacct catacaaggt
    19741 atgtcgaata acatacaggc ccaaaaaggc aatgacaaaa gtcagagtgt tgataaggga
    19801 aatagtttga atacttataa cattgataag gaatgcgcca gccagattgg cgacaatacg
    19861 gacaaccctc atcagagact gcctgaaccc catggcatca tgcaggtcgt ccttaattac
    19921 actgatgtag agggcgttca tcatgtaggc agacaggtag ctcaaggtgt cagagatgag
    19981 gttgatgatg acaatcataa tgactgaaac taatgaaaag ggcgacaaga cgagaaatac
    20041 tgtcaaaata gcgtagagca ggattttgat tttggcaatc cagagtgcgt gtttgactct
    20101 attttcttga aaatctccta gaatcccgag aaagagttgc gatagtaggg gaaccatctc
    20161 tgaaatggaa atcagtaaaa tcgctagcgg agcgaaattg tactgagcca cataattgat
    20221 aatggcgagg tagaaaatac tgtcgccgaa tgatgaaaaa cagctgttta gcagcaactg
    20281 tctatatatg ttgtttgtcc aaaataattt catacttttc tccttatgtc tgcacatcga
    20341 atgggaattt attatttaat agacaccaat ataccccatg cttcctattc cgaagtcaaa
    20401 caaatctgct ttaccactat ctccttgaaa cataagtaaa ccgtgatcat ctcgttcaga
    20461 acatgctaaa attacctgtt ggattttttt catcagtaat ttgttttgat tgtagacagt
    20521 ggtttgtagt aagctagtta ttccgtggca atatcctgtc atcattttta gatgatggtt
    20581 aaaaacaaat tcttgatatt tactaataat atctttgttt ccgtcacaat cgtacataca
    20641 aagatataag attattccgg aaattccttt gcaccaagaa agttgtaaat ttcctaaatt
    20701 ttcagtatta gaagtaactt tttctaataa tttttttaat tcagtatgga atgtatggag
    20761 atcattatag aacatagaag gttctaagac tttagaatag gcaaataaag catatgcaat
    20821 tccacaatat ccatgagcaa agctatagtc taaaatgctt tctttggctg tatctctttg
    20881 taggaaataa tgataaattt cccctatact attgtggatg aaaattttaa gagtattgtc
    20941 attggtaaat tgatagtatt ttattaatga taataggaca ccagattttc ccaatgcaaa
    21001 gtctatttcc tctagagtat cataattctc tattaatttt ttagcggtga cgtttgctaa
    21061 ttgtagatag tattgatttt tactgataga aaataaatgt aggagtaacc atagatagcc
    21121 tgcatcacca aatagcagtg agtattgata ttcatatgtg tgtgtcgttc taattgtcac
    21181 tgccttcttg atgatagaga gtattttaga atccagttct ggggggtaca atttatttag
    21241 aggaaacagt agtccagcta ttccgtgttg gaatgataaa ttacttacga aatttccaaa
    21301 ttctgttgaa gaagtgatcc ttccttcaga atttacagtt ttatctagta aaaattgtag
    21361 aacactatta cattctgttt tgtaatcata tttatgttcc tctatttttt taattttgac
    21421 taaattattt gtttgactta aatttagtag acattgtaac caaaatagtt gctcatctgt
    21481 gagctgacct tgagtatgtg atagttctag taattgaata ggatccttta gttagattca
    21541 atcaatctat aacacaacaa cataataata cttttaggat aacttatcaa atattatagt
    21601 aaaatgagat aa
//
LOCUS       DAVKFH010000026        21122 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKFH010000026 DAVKFH010000000
VERSION     DAVKFH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21122
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.026"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(166..780)
                     /locus_tag="VW006_001173"
     CDS             complement(166..780)
                     /locus_tag="VW006_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9380035.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VW006_001174"
     CDS             complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VW006_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9380036.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2005..2634)
                     /locus_tag="VW006_001175"
     CDS             complement(2005..2634)
                     /locus_tag="VW006_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9380037.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            2872..3072
                     /locus_tag="VW006_001176"
     CDS             2872..3072
                     /locus_tag="VW006_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380038.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            3503..3826
                     /locus_tag="VW006_001177"
     CDS             3503..3826
                     /locus_tag="VW006_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9380039.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3884..5827
                     /gene="thrS"
                     /locus_tag="VW006_001178"
     CDS             3884..5827
                     /gene="thrS"
                     /locus_tag="VW006_001178"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9380040.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5876..6850)
                     /locus_tag="VW006_001179"
     CDS             complement(5876..6850)
                     /locus_tag="VW006_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9380041.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6843..7520)
                     /locus_tag="VW006_001180"
     CDS             complement(6843..7520)
                     /locus_tag="VW006_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380042.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7676..8749
                     /locus_tag="VW006_001181"
     CDS             7676..8749
                     /locus_tag="VW006_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9380043.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8840..9142)
                     /locus_tag="VW006_001182"
     CDS             complement(8840..9142)
                     /locus_tag="VW006_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380044.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9210..9647)
                     /locus_tag="VW006_001183"
     CDS             complement(9210..9647)
                     /locus_tag="VW006_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9380045.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9723..10565)
                     /locus_tag="VW006_001184"
     CDS             complement(9723..10565)
                     /locus_tag="VW006_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9380046.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10677..11327
                     /locus_tag="VW006_001185"
     CDS             10677..11327
                     /locus_tag="VW006_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9380047.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEILE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11537..12172
                     /locus_tag="VW006_001186"
     CDS             11537..12172
                     /locus_tag="VW006_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9380048.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWENYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            12303..12740
                     /locus_tag="VW006_001187"
     CDS             12303..12740
                     /locus_tag="VW006_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380049.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12835..13134)
                     /locus_tag="VW006_001188"
     CDS             complement(12835..13134)
                     /locus_tag="VW006_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380050.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13542..13904)
                     /locus_tag="VW006_001189"
     CDS             complement(13542..13904)
                     /locus_tag="VW006_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380051.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            14263..14677
                     /locus_tag="VW006_001190"
                     /pseudo
     CDS             14263..14677
                     /locus_tag="VW006_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14716..15159)
                     /locus_tag="VW006_001191"
     CDS             complement(14716..15159)
                     /locus_tag="VW006_001191"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9380052.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(15189..17411)
                     /locus_tag="VW006_001192"
     CDS             complement(15189..17411)
                     /locus_tag="VW006_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9380053.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17822..18064
                     /locus_tag="VW006_001193"
     CDS             17822..18064
                     /locus_tag="VW006_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380054.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            18404..19033
                     /locus_tag="VW006_001194"
     CDS             18404..19033
                     /locus_tag="VW006_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9380055.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(19156..21048)
                     /gene="pepO"
                     /locus_tag="VW006_001195"
     CDS             complement(19156..21048)
                     /gene="pepO"
                     /locus_tag="VW006_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9380056.1"
                     /translation="MTRYQDDFYDAINGKWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         6241 a         4730 c         3816 g         6335 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccccct gaaaatcaga
      121 gagctgattt gagctcgggc taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aggataacac
      301 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaacc tcctgtatta gtcagtaata aatcagcaga ttttaggaga gcacagacac
      841 attaatttag ctatcgctag tgggtaatgt cgaacgtagg aaagcatttt actcctcctc
      901 aaaacgtagt tacaaagtaa tttctaactg gaattcagtg actacttcca tgtaaagtat
      961 aacatactat actttacttt gtaaagagtg ggctcttcta gggggcttgc atacagctac
     1021 tcggcttgaa aagccgagta actgcgcacc tttatggtgt aattagctga taccatttga
     1081 ggtttttgta gtctccaaaa ttgttaccga taaaacctca ataaaaaagc cctctgaagt
     1141 cagagagctg attttagccc gggctaaaat cctagtgaaa aagatgaaac tccttgtgct
     1201 catcgaacac tgtgtcattt ccctattttc atacggattt ttgacgccct tagcatcatg
     1261 attcttgcta gataaatcgt ttatagacta ggggacgagg tgaagatcgt acgaaacttt
     1321 cagtgaaaca aaaaaatctc cccgaaggga gaaaagctag tttattttac cttacagtgc
     1381 taggaaccgt aaccgaagat tatacttgag tatagcgagg taaggtgaca acgcaacaaa
     1441 gctacccaat ttagagtagc tttattatca agatatgctc aattgaacac ggcctaagca
     1501 cttggcaaaa aagataaaat ctcctagaaa ctgaagtttc ttcgtcaatt ttcctatttt
     1561 tgctttgtgt ttttgacggc ctttgtatct tgaattagcg acgaagtcct agagagttga
     1621 tcaactcacg gtaacggtta acgtcgtttt tacgcaagta tgcaagcaag ttacgacggc
     1681 gaccgatttt tttcatcaat ccacggtaag tagcgtggtc ttttttgtgt tgtttgatgt
     1741 gttcgttaag gtggttgatt tcccaagtaa ggacagcaac ttgaacctct actgaacctg
     1801 tatcaccttc gtgacgtgca tattgtgcaa tgatttcatt ttttttctct tttgagattg
     1861 ccatgatgtt tctccttttt atttggcttc atccgagtga caggttggca tgcctgtaac
     1921 caagaagaag ttatttgtct ttacgacaat ccctatttta ccaagattta aattttctgt
     1981 caaacattaa tactctcata gttattattt ttcaagtaaa gagatacgtt cttttatatg
     2041 atgacctttt tcaagttcat ctaccaatcc aatagcataa tctgcataac taatctggct
     2101 tattcctttt tcattagtag taaaaatttc tcctgctaga atgtagtttc ctgttttttc
     2161 accatcggga ataaaatcta cagcaggact gacaaaagtc cagttgagat tattttttgt
     2221 acgaagaaga tccaattcat ccgcttgagc ttttgctagt gacttaaatt cttcaggaaa
     2281 atcagacgta tctaaaagtc gtgtcgtttt tgtttcatct atataaagac ttcctgctcc
     2341 ccctacaact agaaaacgtg tctgtgttcc agcaagaagc tgattaaata gttcgattga
     2401 tttgctgtgg agcggtagcg tatctggtgt ataagcacca aacgctgaaa taacagcatc
     2461 aaatccagta agatcatctt ttgtcaactc aaataaatct tttttaataa tagactcaga
     2521 ttgacttttg ttttcagaac gaacaatagc cgttacttca tgtcctcgtt tgactacttc
     2581 ttcaacaatt gctttccccg cttgtccatt tgctgcaata actgctagtt tcatttttta
     2641 tacctctctt attgtaatta ttttagttac aacaattgtg acactcttaa taatcaatgt
     2701 caatagtttt gcttaattat tatcaaaata tttctaccaa gaaaactaac catgattcta
     2761 gtgaaaaaaa aatcttcttt gccaacaaat ttactttctt gttttaaaca tgctataata
     2821 atcatagcaa gagatctaag ttgtctgttt ttttaaaacg aggtgattat catgcgtaga
     2881 ttctattccc atctccccta ctatctggtc atattattct tttattggcc actttatgag
     2941 ttgttcttac tagttgtttc tgaccccctt acactcaagg aactctatat aaacaatctt
     3001 ctcttcttta cacctctggt aatcttgatt gtatcgttac tctatagcta ccgtttccgt
     3061 ttctcacttt gatggttagt tggtaacgga ctgctctttt actttactat cataaccttt
     3121 ggtgagttta tactaattta cttgctaatc tatgaaacag ttgctctggt gggcatggat
     3181 tctggtatta gcatcaagca tattctacaa aaaatgaaaa acaaaaaact ttcacaaaat
     3241 ccttgaaaaa tctcacaatc atgctataat aatccataga gacaagtcac ttagtccctt
     3301 tctactagag agtgcgtggt tgctggaaac gcataggaag tctaaactga tactactctt
     3361 gagtttttta tgaaaacata aaacggtggc cacgttagag ccaatcagag gtgtccctct
     3421 cttttgaggt acataaatga aggtggaacc acgttgcgac gtcctttcga ggatgtcgca
     3481 tttttttatt aggatactaa ttatggagtt gcaagaatta gtggagcgca gttgggcaat
     3541 ccgacaagct tatcacgaac tggaagttaa gcatcatgat tccaagtgga cggtagaaga
     3601 agaccccttg gctttatcta atgatattgg aaatttccaa cgactggtga tgacaaagca
     3661 aggacgctac tatgatgaaa caccctacac actggaacaa aaactttcag aaaatatctg
     3721 gtggctatta gaactttctc aacgtttgga tatagacatt ctgacggaaa tggaaaactt
     3781 cctctctgat aaagaaaagc aattgaacgt taggacttgg aagtagtctg atgataaaaa
     3841 atcaatgctt agaaactatg aaataataaa aaaggagaac atcatgatta acattacttt
     3901 cccagatggc gctgttcgtg aattcgaatc tggcgtaaca acttttgaaa ttgcccaatc
     3961 tatcagcaat tccctagcta aaaaagcctt ggctggtaaa ttcaacggca aactcatcga
     4021 cactactcgc gctatcactg aagatggaag catcgaaatt gtgacacctg atcacgaaga
     4081 tgcccttcca atcttgcgcc actcagcagc tcacttgttc gcccaagcag ctcgtcgtct
     4141 tttcccagac attcacttgg gagttggtcc agccatcgaa gatggtttct actacgatac
     4201 tgacaacaca gctggtcaaa tctctaacga agaccttcct cgtatcgaag aagaaatgca
     4261 aaaaatcgtc aaagaaaact tcccatctat tcgtgaagaa gtgactaaag acgaggcacg
     4321 tgaaatcttc aaaaatgacc cttacaagtt ggaattgatt gaagaacact cagaagacga
     4381 aggcggtttg actatctatc gtcagggtga atatgtagac ctctgccgtg gacctcacgt
     4441 tccatcaaca ggtcgtatcc aaatcttcca ccttctccat gtagctggtg cgtactggcg
     4501 tggaaacagc gacaacgcta tgatgcaacg tatctacggt acagcttggt ttgaaaagaa
     4561 agacttgaaa aactaccttc aaatgcgtga agaagctaag gaacgtgacc accgtaaact
     4621 tggtaaagag cttgacctct ttatgatttc acaagaagtg ggacaaggtt tgccattctg
     4681 gttgccaaat ggtgcgacta tccgtcgtga attggaacgc tacatcgtaa acaaagagtt
     4741 ggcttctggc taccaacacg tctacactcc accacttgct tctgttgagc tttacaagac
     4801 ttctggccac tgggatcatt accaagaaga catgttccca accatggaca tgggtgacgg
     4861 ggaagaattt gtccttcgtc caatgaactg tccgcaccac atccaagttt tcaaacacca
     4921 tgttcactct taccgtgaat tgccaatccg tatcgctgaa atcggtatga tgcaccgtta
     4981 cgaaaaatct ggtgccctca ctggccttca acgtgtacgt gaaatgtcac tcaacgacgg
     5041 tcacctattc gttactccag aacaaatcca agaagaattc caacgtgccc ttcagttgat
     5101 tatcgatgtt tatgaagact tcaacttgac tgactaccgc ttccgcctct ctcttcgtga
     5161 ccctcaagat actcataagt actttgataa cgatgagatg tgggaaaatg cccaaaccat
     5221 gcttcgtgca gctcttgatg aaatgggcgt ggactacttt gaagccgaag gtgaagcggc
     5281 cttctacgga ccaaaattgg atatccagat taaaactgcc cttggaaaag aagaaaccct
     5341 ttctactatc caacttgatt tcttgttgcc agaacgcttc gacctcaaat acatcggagc
     5401 tgatggcgaa gatcaccgtc cagtcatgat ccaccgtggg gttatctcaa ctatggaacg
     5461 cttcacagct atcttgattg agaactacaa gggtgccttc ccaacatggc tggcaccaca
     5521 ccaagtaacc cttatcccag tatctaacga aaaacacgtg gactacgctt gggaagtagc
     5581 caagaaactc cgtgaccgcg gtgtccgtgc agacgtagat gagcgcaatg aaaaaatgca
     5641 gttcaagatc cgtgcttcac aaaccagcaa gattccttac caattaattg ttggagacaa
     5701 agaaatggaa gacgaaacag tcaacgttcg tcgctacggc caaaaagaaa cacaaactgt
     5761 ctcagttgat aattttgttc aagctatcct agctgatatc gccaacaaat cacgcgttga
     5821 gaaataagag tctagcataa aagcctccaa tctggaggct ttttctcatc tatttttact
     5881 caaggactaa gttcacttga gcaaactgaa tccgcactgt cgttcctttt ccgacctcag
     5941 actcgatacg aatctggtgc cccagttctt cagaaatttt cttagataga taaaggccaa
     6001 gtccagagga ctgctgggtc aaacggccat tgtatcctga aaagccacgt tcaaatactc
     6061 ggaggacatc actgtttttt atcccgattc ccgtatcttt gatacaaagc tcttggtcat
     6121 ccatataaat ctccagacca ccttccttgg tgtacttgag actgtttgag atgatttgct
     6181 caataaccac tagcagccac tttttatccg tcacgatttc tttatcaagg tcatgtagat
     6241 tgacatttaa gcctttttga ataaagaaaa gagcatattt acgaattatt tccttgacca
     6301 agtcctcaat ttgaacctgc tttaagacca aatcatcatg gaaactttct aaacgcaggt
     6361 actgtaaaac taggttggta taagagtcga ttttgaaaat ttcctgttct agctgctgct
     6421 tcagttggcg gtcgaccact tctgcaacta agagttgact ggctgcaatg ggggtcttta
     6481 tctgatggac ccacaaggta tagtaatcca gcaaatccgt cagttttctt tctgcttttg
     6541 acctctgctg atagagttcc atctcacgcg cttctaattt ttctgctaaa gctatttcca
     6601 aaggagactt ggcttccctc tctccataga gaagttcctg gcgatagacc tgcatttcca
     6661 ccaatatgtc ccaagtgaaa aataagatgg ttacaaagca acacaagaag aaaaagtaga
     6721 ggaagtaaat tcctagactg gcaaataaaa actgaaagag taagacaaga aatgccaaag
     6781 aaagcagata gataaaaaga cgactacggg agcgcagata ggctagaaaa aattgtttcc
     6841 aatcaagcat gcttcaatcc gtaccctatt cctttcttgg tctcgataaa tcctaccaat
     6901 ccctgctcct ccaacttttt acgcaaacga gccacattga cagagagggt attatcatca
     6961 atgaaaaagt cactgttcca aagttcccgc atcaggtcgt cacgtgctac gatgttgcct
     7021 gcatgctcaa ataacacgcg taaaatctgg aattcattct tggtcaaatt caagacttgc
     7081 ccttgataat gtaaatccat ggatttggta ttgaggataa caccagcata ttccagcaaa
     7141 ctctcatcac gcccaaactc ataggaacga cgcaacaagc cctgaacctt agctaaaaga
     7201 acctgctggt caaaaggctt ggtcacaaag tcatccgccc ccatattgat tgccatgaca
     7261 atatccatag cctggtctct cgaagaaaga aacatgatag gtaccttgga aatcttgcgg
     7321 atttcctgac accagtgata accattaaac aagggcaaac caatatccat gaggaccaga
     7381 tgaggttccg actgaacaaa tagactcaaa acttccataa agtcttctac caggaccact
     7441 tcaaatcccc attcagagag cattttccca atctgttgac gaatgacctg atcatcttct
     7501 attaataaaa tcttgtgcat gcgcttctcc ttttccatta ttataacaga tttttccatg
     7561 ctagatggtc tgaaactgaa tttgaaatag cctgttttta gccagtgcaa acaggctatg
     7621 ctactagcta atttgaggga aatttgctaa gataaataaa aagaaaggag ctcttatggc
     7681 caatattttt gactatctga aagatgtcgc atatgattct tattacgacc ttcccttgaa
     7741 tgagttagac attctaacct taatagaaat cacctacctc tcctttgata atctggtctc
     7801 cacacttcct caacgtcttt tagatctagc acctcaggtt ccaagagagc ccaccatgct
     7861 tactagcaaa aatcgccttc aattattaga taaattggct caacacaagc gcttcaaaaa
     7921 ttgcaaactc tcccatttta tcaacgacat cgaccctgaa ctgcaaaagc aatttgcggc
     7981 tatgacttat cgtgtcagcc tcgataccta tctgattgtc tttcgtggga cagatgacag
     8041 tatcattggc tggaaggaag atttccacct gacctatatg aaggaaattc ctgctcaaaa
     8101 gcacgccctt cgctatttaa agaacttttt tgcccatcat cctaagcaaa aggttattct
     8161 agctgggcat tccaagggag gaaatctcgc tatctatgct gctagccaaa ttgagcaaag
     8221 tttgcaaaat cagatcacag cagtttatac atttgatgca cctggtctcc atcaagaatt
     8281 gacacagact gcgggttatc aaaggataat ggatagaagc aagatattca ttccacaagg
     8341 ttccattatc ggtatgatgc tggaaattcc tgctcaccaa atcatcgttc agagtactgc
     8401 cctgggtggc atcgcccagc acgatacctt tagttggcag attgaggaca agcacttcgt
     8461 ccaactggat aagaccaaca gtgatagcca gcaagtagac acaaccttta aagaatgggt
     8521 ggccacagtc cctgacgaag aacttcagct ctacttcgac ctcttctttg gcactattct
     8581 tgaggctggt attagctcta tcaatgactt ggcttcctta aaggcgcttg aatacattca
     8641 tcatctcttt gtccaagctc aatccctcac tccagaagaa agagaaacct tgggtcgcct
     8701 tacccagtta ttgattgata ctcgttacca ggcatggaaa aatagataat actcttgaaa
     8761 attaaatgta tacaaaacaa aagacctaga atacatactt tcatgtgcat tctaagtctt
     8821 tttaaataga atctaatagt caataaaaat caaagagcat tgagagataa tggggcttgg
     8881 aacgtccctc tcgcttcaac aaaatgaccc cattatagat taaaaagatg ccacttagaa
     8941 aaagcaaaaa aggaagtaag acaaaggcaa atacataaaa agctaactga acattctcgt
     9001 atccattttt ataaaaaagg taggatagat aaaaataact tgaaatgagg gataataaaa
     9061 ataatactgg attccacaaa cttctattat ccttccaaaa tgacactata aaggctaata
     9121 caattcctat aacgagatac atttcttact cctttaatag ctacatttta tcataattat
     9181 ccaaagaaaa aagagggcat ttatccctct taatccttca tctgactctc tgcatcggcc
     9241 acgacttttt ctagactggt ttgaccaagt tctgcctcca tagtcaactg aattctctcc
     9301 aatttttgat ccaaaacatc atgaatatga gctcctacag ggcaatttgg attcggattg
     9361 tcatggaaac tgaagagttg acctgtctta ccaagacatt cgaccgcctg ataaacatct
     9421 aaaagactaa tatccttaag gtccttgaca atatctgttc cgcccgttcc acgcgctact
     9481 gaaatcagct ctgccttctt caactgggac aagatctttc tgataatgac aggattgacc
     9541 ccgacactag cagccagaaa atcactggtc accttgcttt tcttcccctc gagggcaatg
     9601 attatcagca tatgagtcgc aatggtaaat ctacttggaa tttgcatcct cttctccttt
     9661 ttacgaggct accctgcctc tactcttctt tttctattat tatacccttt ttagttgtaa
     9721 tgtcaatcgt taccactttt caaccagtcg tctaactccc gatcgcaacc ctctttctga
     9781 gccaattctc tcaaaaattc ctgatgatga gtatggtgga tcccattgac cagactttca
     9841 tagtaaacct caaaataggg aagtctcagg tctttagcca gctgcaattc agctgccaca
     9901 tcgtaatcta cccgtcggaa gtccatatct accaggccct tgtcatcaaa ctccaaaatc
     9961 atatactggg cccgcaagtc cttccgtagc tgagcgtcca aaaagaaagg ttggccaatc
    10021 gaacccggat tgacaatcaa ttgcccacca gtcccgtaac gaagcaactg ctggtgaatg
    10081 tgtccataaa cagcaatatc acagggagga tgagtcacca agcggtcaaa ttcctcttgt
    10141 ttgccagtat gaatcaactc tcgcccccag ttcttatcag gcagatgatg gctaattccc
    10201 actgtcaaat ccccaaactg acgatgaatt tgaagaggtt gattgtggag cacttcaatt
    10261 tcttctaggg aaatttcctc taaaacatac tggcactggc gcaagagata gcgttgacta
    10321 gggcgagtac tatccaattc cttacggaca ccatgccaaa gactgtcttc ccagtttccc
    10381 aaaactctag ccgtaatcgg tagttgatcc aacaagtcca aaatccttct acgccctgtc
    10441 cctggcatga gaatatctcc caaaagccag tattcatcca ctcctagctg ccgagcatct
    10501 gccaaaacag cctccaaggc ggtggtattt ccatgaatat ctgaaagaag agctattttc
    10561 gtcatatcca tctcctcgtt ttttctcttg caataagtat aacataaaaa gtcacagcta
    10621 gagaaatcta gctttttttg atatactaga taaagatatt agacaagagg aaacgaatga
    10681 ccccaaacaa agaagactat ctaaaatgta tttatgaaat tggcatagac ctgcataaga
    10741 ttaccaacaa ggaaattgcg gctcgcatgc aagtctctcc ccctgccgta actgaaatga
    10801 tcaaacgaat gaaaagtgaa aatctcatcc taaaggacaa ggaatgtggc tatctactga
    10861 ctgacctcgg tctcaaactg gtctctgagc tctatcgtaa gcaccgcttg attgaagttt
    10921 ttctagttca tcatttagac tatacaagtg accagattca cgaggaagct gagatcttgg
    10981 aacacactgt ctctgacctg ttcgtggaaa gactagataa actgctaggt ttccctaaaa
    11041 cctgccccca cggaggaact attcctgcca agggagaact cctcgttgaa atcaataacc
    11101 tcccactagc tgatatcaag gaagctggcg cctaccgcct gactcggatg cacgatagtt
    11161 ttgacattct ccattatctg gacaagcact cacttcacat cggtgaccag ctccaagtta
    11221 agcagtttga tggcttcagc aataccttca ctatcctcag taacgacgag gatttacaag
    11281 tgaatatgga cattgcaaaa caactctatg tcgagaaaat caactaattt ctcaagtccc
    11341 ctaccaaccc tgaaagtttt attttcaggg ttttatttct atcattttat tttactttca
    11401 tttggtggta gaatatttga aaaaaaatgg gagaatattt gaaaaaaaga aaggtagttt
    11461 aacctatgaa aaaatcacta aaaatttttg ctacatctaa atagtttgac ctcttcgggg
    11521 ttgctttggt cgttggattg cgattgcatc tggttacctc aactcacgcc tcgataaatt
    11581 tgtagactgg ggaccatgga cagctcttgt accctttgga ttgatttccg taaccaacgt
    11641 tgggatttcc atgttgtcca ctcgtttcac ggggaaatta agcaaatggg aaaattactt
    11701 tggtattgtt aataccattt tgtccggtgc tattgattat atccttggaa ataaggcggc
    11761 catcattacc tatcccgtca ccttcctcat ttataccttt gctataaaga aatggaaagc
    11821 ttcgcaagaa ggcagaccca accaaatgag ccaaaaacag gtaaaattgg cggccatcat
    11881 catttccatc atcgccttcc tctttgcctt tgtgaccaac tatatcggat atgggggcaa
    11941 gatgaatctc cttgcctacg taacaactat tgcctttgca ctgtccctca ttgccaatgc
    12001 tttgaatgca ttaaaactaa caactcagtg gggcttttgg ttgatttaca atttcgttca
    12061 gctgacaaaa gctggtattc aaggaaactt cgccaatatc ggtaaataca tcttttatat
    12121 cctcaatgca atcggagctt tatttgtctg gaatgatgaa gaagttagat aatagaagga
    12181 aactcaaata aagaatacta gccaacttaa gagaatctcc cctctgctat ttttatcatt
    12241 ttagttgtaa ctcccattct tttagagtag aatgaagtta gataaaagag ggaggtcttg
    12301 tcatgaaaaa actctataga atccatttta tagcaattgc agttattgat ttgctactat
    12361 ttgcattttt tatcactaga ctcgagacat cttttgagtg gctcttgctc tctggtttaa
    12421 ttttcttcct tgctcaagga ctcctgctat ttctcttggt cgtccgactc aaacatcaat
    12481 tcgctgagat ttatcctcaa atcaataaaa agattcgctt ctactattta ggggttctca
    12541 ccattgattt tctatttttt gttctcttag ccttcattag ttctcagcgt ttttcatctc
    12601 ttatgccaat catcactgct tgccattcta ctttttatta tatgacagct gactacctaa
    12661 gagaaaacta tccagacttt tacgacaaac acatctctaa cacgtatact attttatcga
    12721 tttcttcttt tttagtataa gacagctcgt tctccagaaa tattcattaa ctttcatttt
    12781 tatacttttc ctttcgattt tcaaaaacaa ggggagggat taacctccct tatattagac
    12841 ataactttta ggatcacaat tgtatgttcc tttcgaaata tccccaaaaa tcgtagcccc
    12901 tgttaaataa tgatttcttg tagaagcata tgctattttc ttcatcacat ccagtctcgc
    12961 tacttttagc tcgagattag gattggaaaa catagcattt ttaatatctt ccaaatcttg
    13021 agaactaggt gcgagtagag ttacatcgcc ctccaatcta ttagccttgt ctatataaag
    13081 actaactctt tcagcacttt ctgttcgcag aaaatttagt acatttctat tcattttttt
    13141 acctcgtatt tttctaatta ttgatttttt ctagaaacaa aatcaattca acggtgaagc
    13201 tctagctttc atcttcattc gtacctcctt ttgtgtatat ctagctttac ataaacctat
    13261 gatttagagt atagtttctc aattattctc agaaactatg gagatgattt aattatcata
    13321 caaatcaaag acctctcctt gctcatcttc accgtaactc tcgagttcct cataaaagta
    13381 ttccaaagca gtttgttctt ctggtgtcat aaagaaactc ctttctaaac aatattagta
    13441 aaaaatacgt tcttatttcc atcctaaata aagtagtgtt tttatcccgt aataggtttt
    13501 atttttgcgg agcattacac tccgcattaa tattgatagt attaagaatc attattactt
    13561 aataaaaatt gcgctctcaa acatagacgt atacaaaatg ccctatgtat acgtttcgct
    13621 tggaagacat ctaagaaaaa attttgtagt tcagacagta agattaatac atctttctct
    13681 aacataagtg atgtctggta ctcccaatta ttgctctgtt ttaacgatga aaaaggtata
    13741 gctgtctctt ttaattttat ccaatctata cgttcttttt taggaacgta ttctctaaca
    13801 gcttgactaa caatactgga atagcttact tcatagccta atttattgta aacatcatct
    13861 ttaagcttat ctaatatctg tctggattca gacgctaaac gcattctaac attctctttt
    13921 cccatattat cacccctttc tgacgggatt ctgttatccc gtaaattaag tatataacaa
    13981 tttagaaaaa aaatcaagaa ttaagttgaa aaaaattcca caatctaaaa atttatcatt
    14041 ttatgacaaa catactatcg ttaaatgctg atttaatcga aattttgagt tataaactcc
    14101 tcagtttcga actcaaaatt agccgtgcag gataggatac ccttcataaa cttatataac
    14161 tactttaata ccttaaaaag ctaataaaat aaggaatatc taagcaatat acattctaaa
    14221 atacctgtag gggagtgtct ctaaggaaaa ggaggtttta gcatgaaaaa aatcatcttc
    14281 atcaaaacca ttcaactcct tgtcattgat ggaatcatgc tggtattttt gacatttaaa
    14341 aggggggctt acttgggact ggattttgat ttatagtggt tggctcattt tctttcatcc
    14401 cgtgctattg acctatcttt caaaccaact ttgtgaccac tttagtcaac tctattccca
    14461 gattagaccg agattctggc gttttgcttt acaaattctc ctatgggata gcctgatgat
    14521 tctctccttg gtgtctttaa gtgatattcc acttttcctt cagggaactc tcctcatcct
    14581 aggacatctc atcccttcct atcgcatctg ccaaagcctg aaaagagact tcccccaagc
    14641 atatcaagaa ccgatttctt tttggagtat tttatgatcg atgagaaaga ccaagccgac
    14701 tgggcttggt ctttcttatc tctttttagt atctaggata atggtaacag gtccgttatt
    14761 aaccagctca acctgcatat ctgctccaaa gatacctgtc tgaacgggca cttcttgcgc
    14821 taatttttga ttgaaagcat catagaagtc tgatgccata tcaggtttag ctgcccctgt
    14881 aaaggctgga cgattgcctt tcttagtatc cgcaaagagg gtaaactgag aaatggagag
    14941 gatttctcct tcaatatctt tgacagacag gttcatcttg ccttctgcgt ctgaaaaaat
    15001 ccgcatattg accagttttc tcacagcata gtccaaatct tcctcttggt cctctggtcc
    15061 aacaccaacc agcaataaaa gtccctgatt gatttttccc tgaatctggc cttctatact
    15121 cacttgggct tttttaaccc gttggataat gattttcata atagcctttc tagtaagagc
    15181 taggacaact agccgttggt ccgtttgaca gagtaaactt ctggcacact cttaatttta
    15241 tcgacaaccg tggtcagtgt agagaggttg gcaataccga aggacacatg gatattagca
    15301 aacttcatat ccttggttgg ttgggcattg accgttgaaa tattcttggt tgtatttgaa
    15361 agaacttgca gtacatcgtt caacagtcct gtacggttga gaccgtagat atcgatatgg
    15421 gccatatact ccttatttga gctagagtac tggtcttccc attccacatc aaggagacgt
    15481 tgctcgtagt tttcttgggc acgcaggttc atacagtcca cacggtgaat agccacacca
    15541 cgacccttgg taatgtagcc aacaatatcg tcaccaggca cggggttaca acacttagca
    15601 atccgcacta ggagaccaga agcaccttca ataaccactc ccccctcatg cttgaccttg
    15661 agagtttctt tattttcaac cttgacctcg ccacctttga caagctcctc tgcctcagcc
    15721 ttggccttgg cacgctcttc ctcacggcgt tccttttcag tcagacggtt aaagacggta
    15781 atcgcaccga tttccccaaa accaatggcc gcaaagaggg agtcttctgt cttgtaactg
    15841 gtcttttgca gaacttgatc catgtggcgc ttgtccataa atttatttgc cacatagcca
    15901 ttttcttgga actgagccat cagcatctca cgacccttgt tgacagacaa ttccttatct
    15961 tggtttttaa agaactggcg aatcttattg cgcgccttgc tagtcttgac catattgagc
    16021 cagtcacggc taggtccaaa ggagttcggg ttggcgataa tttcaacctg atcccctgtc
    16081 tttaacttgg ttgtcagtgg aaccatgcgg ccattgacct tggcaccagt tgctttttca
    16141 ccgaccttgg tatggatttc gtaggcaaaa tcaatcggtc ctgaatcttt gggaagggaa
    16201 cggacagctc catctggggt aaaaacgtaa atctcctcag ccaaatagtt ttccttaaca
    16261 gagtccacaa attccttagc atcatcagcc tggtcttgga gctccatcat ctccttgatc
    16321 cagttcattc caatagctga ttccttgctg ttaacttgcc cctttatacc tttcttataa
    16381 gcccagtgag ccgcaacccc gtactcagcc acctcgtgca tttccttggt tcgaatctgg
    16441 aattcaatcg gcccttttgg tccataaaca gtcgtatgga tagactgata accattggcc
    16501 ttgcggttgg caatatagtc tttgaagcga cctggcatcg gtttccaaaa ttcatgcacg
    16561 taaccaagca tggcataaac atcactttgg gtatctaaaa tacaacgaat agcaatcaga
    16621 tcatagattt cctcaaaccg ttttctcttg tcctgcattt tgcggaaaat tgagtaaata
    16681 tgcttgggac gaccataaat cttccctttc aagtgacgtt ctgtcgtata ctcctctaat
    16741 tttgtgacta cctcatccac caaggcctca cgctccctgc gcttttcctt catcatatgg
    16801 gtaatcttgt aaaactccgt tggattgaga taacggaaag acaagtcttc taattcccat
    16861 ttgacactgg aaatccccaa acgatgggca agtggggcat agatttccat ggtttctttg
    16921 gaaatacgct cctgcttgtc ttttcgaaga tgtttcaggg tccgcatatt gtgcaagcgg
    16981 tcagacagtt tgaccaaaat aacgcggatg tcctcagaca tggccatgag catcttgcga
    17041 tgattttccg ctaattgctc ctcgatcgat ttgtactcga ccttgccaag cttggtaact
    17101 ccgtcaacaa tcacccgcac atcaggacca aactctcttt ccaaatcgtc caaggtcgca
    17161 tctgtatctt ccaccacatc atgcaagaat ccacaagcta ctgttacagc atccagcttt
    17221 agcttagcta aaatacctgc cacttggata gggtgaatga tataaggctc gcctgatttg
    17281 cgatattgac cactgtggca ttcaacagca tagaccaagg ccttatggac aaaatgaaca
    17341 tcctcttccg ttaaatattc tttggttaaa gcgacaactt cttcgcctgt taaattcact
    17401 tctttcggca tctctactct ccaattcttc ctaccatttt atcacttttt taagaatatg
    17461 aaaactagat tggaacagaa taagaaaaaa ataattcaaa attgcttgat aattctgaat
    17521 tattggccca taatatacta cgaagttaga ttttaaactt aggtgataga aggagagata
    17581 gaagaacgga aaccatattg taacccaaag actttctgat ttccccaatt ccattgaaga
    17641 tacgaaagat aaacggtgga actcgtatca catacactgg taccttgact ggattttgga
    17701 attaatactc aatgaaaatc aaagagcaaa ctaggaaact agccacaggt tgctcaaagc
    17761 actgctttga ggttgtagat aagactgaca aagtcagtca catatatacg gcaaggcgac
    17821 gttgacgcgg tttgaagaga tttttgaaga gtataaaaat cctcaagata ctttcttcta
    17881 tcctttagtt tataaggaga atacctatga aaaaactgct atttctatct ttgctctcct
    17941 aatgttagga gtttgctgcc tgttcctatt cagccagcaa agctataaaa aactcgttca
    18001 atactatgct aacgaccaga acctgcccag taggataact tatagtgaat atagcgacaa
    18061 atgagaagcc aactacggta gcactctaaa catcacgtct atcaaacaag ctaatgacgg
    18121 agtttatgca acctatgaag ggcaattgac acctttccaa tattgataaa ttgataacca
    18181 gcctgtcttc atctagtcat gctggttttt aagttcattt taaatcctta cctattctcc
    18241 ctaactgtgc tatacttaat ttatactcaa tgaaaatcaa agagcaaact agaaagctag
    18301 ccgcaggctg ttcaaagcac tgctttgagg ttgcagataa agttgacgcg gtttgaagag
    18361 attttcgaag agtattagta cattctttga gattggagtt agtatgaaaa tccataaaac
    18421 cgtgaatcct gttgcctatg aaaataccta ttatctagaa ggcgaaaagc acctcatcgt
    18481 cgtcgatcct ggtagtcatt gggaagccat tcgtcagaca atcgagaaga tcaacaaacc
    18541 gatctgtgct attctcttga cccacgccca ttatgaccat atcatgagtc tggacttggt
    18601 tcgcgagacg tttggcaatc ctcctgtcta tatcgcagag agcgaagcca gctggctcta
    18661 cactcctgtc gataatctct ccggtctccc tcgccacgat gatatggcag atgtggtcac
    18721 aaaacctgca gaacacacct ttgtctttca cgaagaatac caactagagg aatttcgttt
    18781 taaggttcta ccgaccccag ggcactctat cggtggtgtt tccctagtct ttcctgatgc
    18841 tcacctagtc ttgacgggag atgctctatt ccgcgaaact atcggacgga ccgaccttcc
    18901 gactggtagc atggagcaac tccttcatag tatccagacc caactcttca ccctaccaaa
    18961 ctacgatgtc tatccaggac atggtccagc tactactatc gctcacgaaa aggccttcaa
    19021 tccctttttc tagcaagatg atgacaatcg aaatttaagt aaactatcca gcaaatcttt
    19081 ctattacaaa aggcatccta tcaaggtttt cacacgtgat tggatgcctt ttttctgatg
    19141 actagatttt ttgtattacc aaataatcac gcgctcctct ggtgaacgcc acattccgtc
    19201 tccttctttg acatcatagg ttgtaaagaa atcgtcgaag tttggtactt gcacattgac
    19261 acggagtttg gctggtgcgt gcacatcgac gctagccaaa agtttcataa attctggtcg
    19321 acctttcatg cgccagatgc gaccgaagtt gtagaagaac tcttctgctg agaagtctgc
    19381 ttctctctta gctgcttcaa gcgctgctgc gattcctccc aagtcagcca cgttttctga
    19441 tacagtcaat ttaccgttaa tggttgctcc ataagaatcc tgtccatcaa attggtcaat
    19501 gactttttgt gttttctcct tgaaggcagc atagtcgctc tctgtccacc aatccttgag
    19561 gctaccattt tcgtcaaagg aagccccgtt agtatcaaag gcgtgggaaa tttcatgggc
    19621 aatcactgcc ccaataccac cgtagttagc agaagatgac tgatgcaagt catagaaagg
    19681 cgcctgtaaa atggccgctg gaaagacaat caggttcttc tgaggattgt agtaggcatt
    19741 gaccatatga gcaggcatgc cccattcctt atagtctaca ggctggttcc acttactcca
    19801 actgtgcttg atttccacac gcgcaaaggc tagagcattc tcaaaaagac tggcagtttc
    19861 attcactacc ttatccttgt aacgtgcagg caattcttct ggatagccaa tataaggttt
    19921 gatcacattg agcttcacga tagcctgttt acaggtttct ggagtgagcc agtcattctt
    19981 aagcagacgc tccttataga catcaatcat ggttgccact tttttctcca catccgcctt
    20041 ggcttctgga gagaacttct cacgggcgta ccaaagcccc agggcttgct tgaaaggttc
    20101 ctgtgctaga tgataagctg ctttgacctt atcttttgcc tctggaactc cagaaagggc
    20161 acggctgtag gcaccagaca aaacacggat atcctctgtt aaatagctgg ttgaaagatt
    20221 gacaacactc aaaatcaagg ttgctttaag gagagaccag gcttcctcac tgtaaaattg
    20281 ctctgctgct tgccagaaac gttcctcgtc tacaataacc ttgtctggta attgcccaat
    20341 aactgctttg aagaagtcat ccaaaggtag ggcaggcgcg aatttcttga aatcttcgta
    20401 agaatatgga tgatagagtt tagcatattc tgaactttct tcattagaga gcaccactgc
    20461 cgcaactcgg cggtccaatt caagtctttt ttctagcaag tcttcaattt cttcatcaga
    20521 gaaatcataa gccttgagga gatttgcgct gctttctttc caaagagtca agagctcttc
    20581 gcgctgagga tgttcttctg catagtaggt cgtatctggc aagattgtgc ttggagcgct
    20641 agcccataga acattgattc tagcatccat aaagtctggc gatacaccaa aaggaaggaa
    20701 gtttggtttt cctgcaagct caaactctgc tagtttagct gtaaaatccg caaaagtttc
    20761 caattcttgg aattctttaa ggagtggtaa gacaggtgtg ataccgtcag cttctctctt
    20821 gtcaaaatca cgaactaggc ggtggtattt gacaaagttt tccaagatag catcctcagg
    20881 cacttcttca cctgctaacc acttgtctgt tgtcgccagc atcaggtctt caatttcctg
    20941 gtctaaatca acaaaacctc ctgtttgaga cttatctgct gggatttcag ctgtctgttg
    21001 ccattttcca ttgatagcat cataaaaatc atcttgataa cgtgtcatct tgttctcgct
    21061 ttcatttgta tttgcattta tcttaacaaa aatcactagg gaatgcaatt aataaccatt
    21121 ta
//
LOCUS       DAVKFH010000027        20454 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKFH010000027 DAVKFH010000000
VERSION     DAVKFH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.027"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(96..245)
                     /locus_tag="VW006_001196"
                     /pseudo
     CDS             complement(96..245)
                     /locus_tag="VW006_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(399..2030)
                     /gene="rlmD"
                     /locus_tag="VW006_001197"
     CDS             complement(399..2030)
                     /gene="rlmD"
                     /locus_tag="VW006_001197"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9380057.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLDMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2104..3078)
                     /locus_tag="VW006_001198"
     CDS             complement(2104..3078)
                     /locus_tag="VW006_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9380058.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3152..3766)
                     /gene="pmp23"
                     /locus_tag="VW006_001199"
     CDS             complement(3152..3766)
                     /gene="pmp23"
                     /locus_tag="VW006_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9380059.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVQLNLYIIKCFTLFSTSG"
     gene            complement(3766..4740)
                     /locus_tag="VW006_001200"
     CDS             complement(3766..4740)
                     /locus_tag="VW006_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9380060.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4748..6004)
                     /locus_tag="VW006_001201"
     CDS             complement(4748..6004)
                     /locus_tag="VW006_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9380061.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6066..6497)
                     /locus_tag="VW006_001202"
     CDS             complement(6066..6497)
                     /locus_tag="VW006_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380062.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6499..7101)
                     /locus_tag="VW006_001203"
     CDS             complement(6499..7101)
                     /locus_tag="VW006_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9380063.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7085..7924)
                     /gene="prmC"
                     /locus_tag="VW006_001204"
     CDS             complement(7085..7924)
                     /gene="prmC"
                     /locus_tag="VW006_001204"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9380064.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7924..9003)
                     /gene="prfA"
                     /locus_tag="VW006_001205"
     CDS             complement(7924..9003)
                     /gene="prfA"
                     /locus_tag="VW006_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9380065.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9000..9590)
                     /locus_tag="VW006_001206"
     CDS             complement(9000..9590)
                     /locus_tag="VW006_001206"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9380066.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9713..9895
                     /locus_tag="VW006_001207"
     CDS             9713..9895
                     /locus_tag="VW006_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9380067.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10031..11404
                     /gene="mnmE"
                     /locus_tag="VW006_001208"
     CDS             10031..11404
                     /gene="mnmE"
                     /locus_tag="VW006_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9380068.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11451..12386)
                     /gene="dapA"
                     /locus_tag="VW006_001209"
     CDS             complement(11451..12386)
                     /gene="dapA"
                     /locus_tag="VW006_001209"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9380069.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12442..13518)
                     /locus_tag="VW006_001210"
     CDS             complement(12442..13518)
                     /locus_tag="VW006_001210"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9380070.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14149..14221)
                     /locus_tag="VW006_001211"
     tRNA            complement(14149..14221)
                     /locus_tag="VW006_001211"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14186..14188),aa:Thr,seq:ggt)
     gene            complement(14321..14830)
                     /locus_tag="VW006_001212"
     CDS             complement(14321..14830)
                     /locus_tag="VW006_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9380071.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14828..14924)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14953..15384
                     /locus_tag="VW006_001213"
     CDS             14953..15384
                     /locus_tag="VW006_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9380072.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15466..15843
                     /gene="mscL"
                     /locus_tag="VW006_001214"
     CDS             15466..15843
                     /gene="mscL"
                     /locus_tag="VW006_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9380073.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15885..16979)
                     /gene="hemH"
                     /locus_tag="VW006_001215"
     CDS             complement(15885..16979)
                     /gene="hemH"
                     /locus_tag="VW006_001215"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9380074.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17134..18357
                     /gene="pepT"
                     /locus_tag="VW006_001216"
     CDS             17134..18357
                     /gene="pepT"
                     /locus_tag="VW006_001216"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9380075.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18393..19022)
                     /locus_tag="VW006_001217"
     CDS             complement(18393..19022)
                     /locus_tag="VW006_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380076.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            18987..19208
                     /locus_tag="VW006_001218"
     CDS             18987..19208
                     /locus_tag="VW006_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380077.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19285..19826)
                     /locus_tag="VW006_001219"
                     /pseudo
     CDS             complement(19285..19826)
                     /locus_tag="VW006_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19930..20454)
                     /locus_tag="VW006_001220"
                     /pseudo
     CDS             complement(19930..20454)
                     /locus_tag="VW006_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5895 a         4306 c         3860 g         6393 t
ORIGIN      
        1 ctatttattt tttgagtttt aaactttggt agaagtcttg aactccttat atttacataa
       61 ctatttgcct ctataactgt tatgacacga aaccaacaag caagttcaag cacctccata
      121 tctttattca aatgaggact aagatattca caaactttat cataaacctc tttatctttt
      181 ttcataaaag gggcatacaa cttagcaaat ttatcctaaa attttttgtt gcctttatca
      241 atcatttcaa cgctccttta aatataaaaa tgatcctatc aatctttata ttgagcctat
      301 tttctaattg ataccttgca agaaaggaat aatagctgtt cctataccaa gtaggaatgt
      361 aataaatgca gaaagtcata tactgtcatt cttttctatt aaatcatttt gaagtgtctc
      421 aaagcatcca tgatggcttc ttctttttca ggtgtacact gtggaataat ttgtttatcc
      481 tttttagact tgttgtaatg ttctcgtaat tctattccac atttcttttt tatctgtgca
      541 atatataatg tcgaaacttt taattcaaat ttattccaaa catattcttt gatttgagca
      601 tatgttgctt tgctctccgc acttgtcaaa tccatctcat ccagctcaat ttcaacactt
      661 atgtgcttat cgacatcgag tttggacaaa agtgctaccg tctcgacgtg atgcgtttga
      721 ggaaatagat ccaccggctg gactttcttc aattcatatc ccaactcttg gtatagttta
      781 atatcacgcg ccatggttgc gacattgcag gagatatagg cgatgcgatc ggctcctgtt
      841 tgggcgcttg ctttgataaa gctttctgtc aagcccttgc gtggaggatc aaccaagata
      901 acggttggtt gaataccttc cttgagccat ttcttcatgg cattttcagc cgtgtcacag
      961 acatagtggg cattagtaat cttgttcaaa gaagcattct tctggctatt ctctactgct
     1021 tctggaatca gttcaacacc gtagacttct ttgacatgct tggcgactga taaaccaatg
     1081 gttccaatac cagaataggc atcaataatc acatcatctt tttttaactc tgcaaagtca
     1141 atggctgttt gatagagttt ctccgccatt tcagtattga cttggtaaaa ggctgggcca
     1201 gcgatttgga agtcatttcc caacatctgg tccgtaatat agtcttgacc ataaagagtg
     1261 cgccactcct taccaaaaat cgcattggta ttctggtcgt tgatattttg catgacagac
     1321 acaatctctg ggaactgctt gataacttgt tcaatcaatt ggtcaacacg aaaaactttt
     1381 ggacgagttg tcaccaaaac gaccatgatt tgtcctgaat agtgaccacg acgcaccaca
     1441 agattccgaa tcaatccaga ttgttccttt tcgtcataag gttttaaatc aaaacgacgg
     1501 agcaggtctc gaagagctac tacgacttgg tcaatgacag gatcctggat aaagaaatct
     1561 tcaaggggca tgaggttatg cgaattctta cggaaaaatc ctgtttccaa gacaccattc
     1621 actcgacgaa cgggcacctg cgccttattg cgatacttga ctggatgttc catatcaagc
     1681 gtttcagcaa cttctacatc tgcaattcca gcaatcttgt agagactgtc cttgacttgc
     1741 ttggttttaa acttgagctg ttctggataa gaaaggtgtc ctaaatccgc gattcctgaa
     1801 cgcaggtaag ccaaatctag atcttgatta cggtgtggtg actggacaag gtatttttca
     1861 acttttccaa agccaatctt tttattgacc ttgaggacac gcatgagaat tttttcactc
     1921 ggtaaagcat tctctacaaa aaagaccaaa ccatctacct tggcaactcc tgccccttca
     1981 tgggtcaaat caacaatttc aacttctaca atatcatttt tctttaacat tcttttctct
     2041 ttctattggc cgacaaaaaa gacggcaacg ttacggttac catccattat accatgaaat
     2101 ttcttaagaa ccaaaactct tgaagaagtt gacaagggct tgccagaggg agcttaagaa
     2161 gttaccaccg tcctctagcg ccttatccgc atcaaagtta aggttgatat ttttaaaact
     2221 gtcgccagct tgtgatacga tgctttgttt aaggtcattt agggttttag tgaaatctgc
     2281 attgctgagg atatcactct ttgagagatt caaggcaaaa ttgatgatga tattgatctg
     2341 gtttcctgtt atgacctgat caagtttgta attttttaag gtatcttcaa caatcttgcg
     2401 tacatcttct tctgtcagat ttcccttgct ttctttagct ttggcgagtc ctgacttgat
     2461 atcagctagg gcaacgttta atttattagc atcatagcct gatttgtcct tgttttcagc
     2521 attgatatct gacaaagcct ttagctcttc ttgagccaaa tctttattgg cttgcggcac
     2581 cttggctcca ttagcttcta gcgaatagta gatcccagcc aaagcactct cacctgtaac
     2641 tggaataggg gctgctacag tgattttggc atgttccata cccagcgtta ctgctgcgtt
     2701 tcggtacata tcctgagtca ccttagtgat attttctggt gtttcaatct tgacctcaag
     2761 tggcgatttg tcacctagct tttgaatctt ggctgatgaa tacaactgta agctagagtc
     2821 attggccaca ttcatgattt tagaataaac atcaggtgtc atggtcttga gttctttggt
     2881 atctgttgag gcattgtagc ccagtttttt aagagtttga tttttttggt cttcagatag
     2941 ggaggaacct aggacatatt caggttggac ataggtttca tcgataactt tttgaacatc
     3001 tgttgctgca tggacgctat tcatagctgt tactgcccac aagaccgcag cactagtcag
     3061 aaagagtttc tttctcatag ggaatttcct cctttacctt tctagagtaa tatatctatc
     3121 ttaaagaaaa cttataacaa aaacacctga actagccaga tgttgaaaag agagtgaaac
     3181 atttgatgat gtaaaggttg agttgcacct gtctagaata ataatagttt cctccattta
     3241 catagagttc ggcaccgtga aaaatggaaa tgggatgaat ataactataa gtctttccag
     3301 tcgtattacc aagcaagggg gcaacagtct cacgagagta ctgtttggct agagccaagg
     3361 tattttcctt gccattttgg gcgataaaat cgatataggc aggtccaaaa ttataggctt
     3421 gaacagctgt ccagatatct acacccttct tctgcgccag atagagattg cctgtcagag
     3481 tttgaatgcc ttgccgaatg ctagaggcat tatcattgat ggtgttggtg gaaccacttg
     3541 cagactcact agactgcata acatcgcctt cttttccttt tgtttcagta taaatcatag
     3601 caagcacaag ctcttcgttt gctggggtgt ctttttcact caatatttct cgcaccatgg
     3661 gttgataggt catgacttgt ttgacatctt gatgaacgcg gtaagcttta tagccagcaa
     3721 aaaggaagac tgctagtaca agcactcttc gaattcgttt aaacattatt tactttggat
     3781 atcctcgata tttttgatta agatagagta ggttccattt tcgttttgga taaactcaac
     3841 agactcggcg tcttgataga cgttattggg aacgatgagc tcaattccat ttgataagga
     3901 gagtttttgg ttttcaaatt tctttaattg gcgactgaca tcaatttcat caaattgaac
     3961 aggttctggt acggcttctt tgacttggtc aataaagctc aaacgagccg tcagattgtt
     4021 gtcaaaaagg tcattagcca atttctcagg tgacaattca ttgctttctt ctaggttgtt
     4081 gaaaatagct gatttgacct tggattgaaa ttgaaaatca tctgtgttaa aagattcagc
     4141 aattctctgg gctgtttttt ccagttcctt gatagatttt ttaggagaaa tcttaggagc
     4201 gacagcaaga agattatctg aaaaatagtt caaaaaagtc ccgttgtact tgattcgttt
     4261 ttcaatcagg tgatacttgc tactctgaag attgaccacc aaggcctcat cagctcctgt
     4321 tccaaatcca ggcaggttat tctgagttag cttgattgga ttatcaactt ctcctccgag
     4381 gtgggtcaag gtctcccgca gggcaattcg caagaaagcg aaatgttcta caccttcttt
     4441 agaaaattga acaaaaatca agtcattggt cttgagattt tcagaaatgc taaactcctc
     4501 tttccagaga ttagccagcg ttactgatgt ctccaacaaa tcgtctgtaa tatgattgaa
     4561 gaagggattt tcttcttcga aaatcccagt cttggcttca tctgaataca catgttcaat
     4621 ttttttacgc aggtattctt cgatttttgg agtaatattg agaaacttat ctgctaagaa
     4681 cagttcggta tcatccggac tgaactggtg aataatggct ttcttaatat aaatgtccat
     4741 aaaagtttta gtcctcgtat agtaggaagg catctgtcaa ttctttgact gcacttctca
     4801 cttcttctaa tacagcctca ttttctgaat tcttaagggt tttaatgatg agttcagcca
     4861 ctttgcgact ttcttcttca ccaaatccac gtgcagtaat ggctgctgct ccgatacgaa
     4921 tcccacttgt cttgaatggt gacaagcttt cgtaagggat tgagttttta tttaaggtaa
     4981 tattgacttc atccaacaag ttttgagcaa ctttgccgtt ttctacaact ttagtcacat
     5041 caacaaggaa gagatggttt tcagttccac ctgaaataat acggaaatca gggtcttgca
     5101 agaagacatc tgccatagcc ttgctgttct taattacatt ggcagcatat tccttgaagg
     5161 ctggatccaa aacttctttg aaggaaactg ccttagccgc cacaacatgc tctaaaggac
     5221 cgccctgaat acctgggaaa atagctgaat tgattttttt agcaagttct tcgtcattgg
     5281 tcaaaatcaa accaccacga ggtccacgaa gggttttgtg ggtcgttgtt gttgtgatat
     5341 gagcgtatgg aactgggctt ggatgaaggc cagccgcaac caaaccagcg atatgggcca
     5401 tgtccaccat gagcttcgca ccgacagcat ctgcgatttc acggaatttt gaaaaatcga
     5461 taatttgaga atatgctgaa gcaccagcta caatcagttt tggttttact tcttgggctt
     5521 gtttcaagat agcatcaaag tctaagagtt ccgtttcagg atcaacacta taagaaacaa
     5581 agttgtaggt ttgaccagag aagctaacag gagccccatg agtcaaatga ccacctgatg
     5641 ccaaatccat tcccataacc gtatcacctg gctcaatcaa ggacatgtaa gccgcacagt
     5701 tagcttggct tcctgaatgt ggttggacat tggcaaattt agcaccgaaa atttcttttg
     5761 cgcgttcaat agcaagagtc tctacaacgt ctactacatc agttccacca taataacggc
     5821 gtcctgggta accctcggca tatttatttg tcaagataga cccttgagct gccataacag
     5881 ccttggaaac tacgttttcc gaagcaatta actcgatatt attttgttgg cgttcttctt
     5941 ctttggcaat agcattccag agatcagcat catatgcttt aaaatcatct ttgtcaaaaa
     6001 tcataggtct tctcctttat tgtgtgacta gtccattagt ttgattttac aataagaaaa
     6061 tcaaactaaa agatgcgaat aaaccgtttc tgcattttat cacaagtata gccaactttt
     6121 tcataaaatg catgagcacc cagacgatga ttggcagaat ttaagcggat aaacccataa
     6181 ccacatcttt ttgcttcttc ttccaaccct tgtagtaaac ttttaccaat accttgacct
     6241 tgcgcttgag gtgaaactgc taaagctaag atattaaatc ctgctttgga atagagtgat
     6301 tcgtaaactt cagcgtggac atatccaagt aagacatgat tagctgcatc ctcatagcca
     6361 agtaggaaat gatgggaatc ctgagacagt ctagctagtt ggctagccgt ttcctctgga
     6421 ctaaaagtat aacccaaagc ctcttggttg atgtcacata tcgctttcac atctgtttct
     6481 tgcaaatctc ttagcatttc aagcctcctc aaaagaaatc tctggcaacc gagcaagaat
     6541 atcttctcgt ttaattgcgc cttggcgtaa gattttcacc ttgtctccag acaaatccac
     6601 aatagttgaa tcctgtccag ttagaaaagc atcgtcttcc agacccagaa cctcttggtc
     6661 aaaatccttc agaatttgtt caaaggttac accacttgcc tgacctgaga tattggcaga
     6721 cggcccaatc aagggacctg tctctcgaat taaatccagt gtgatagggt gactgggcat
     6781 ccgaaatcca atagttgcaa ggtcagaatt tacccaatag ggaactcggt cattggcttc
     6841 gagaataatg gtcaagggac ctggcaaaaa ggtctctaca agtttttgta gataagttgg
     6901 ctgattctta gaaaagtgca agatatcctc gaaagaggcg atattgagat tgagtgcctt
     6961 atctctagga cgacgtttga gttggtaaac atggtcaact gctttttcat ctaaggcctt
     7021 ggcaaaaaga ccataaacag tctctgtagg tagaacgaca gctccaccat tttccaactc
     7081 ttgtctaatc ctgtccatca tcaaccacaa ccatcctatt ttgaccaaat tggtctttga
     7141 gtgttcgaac ttttttttca ggaagatgtt ttctaaaaag ttcgggaaca ctttgacctt
     7201 gcttgtatcc aatttcaagg taaatcttac caccatcctt gagatagtct tttgcatctt
     7261 ccgcaattct acagtaaata gctaggccat cctcgtctgc aaagagagct agatgaggct
     7321 ccgaatgcaa aacattcaaa cctacctctg actcatcttc acgagagata tagggtggat
     7381 tggatacaat tatatcatat ttttcagaaa tttctgcaaa acaatcagat tttttaaaaa
     7441 atatattaag attttgatcg ctagcattct ctgatgcaag ctctaaggcc tcttgagaaa
     7501 catctgctgc tgtcactgac caatctggtc tattttttgc tagagcgaga gcaatagctc
     7561 cactacctgt tccaatatcc agaactgaga gattcgtctc aagattttca gttagaataa
     7621 gctccactaa ctcctctgtt tctggacgag gaatcaaaac ccgttcatcc acctttaaat
     7681 gcattccata aaaatctgcc tgtccaatga tgtactgagc tggcttgtga gctgctagtt
     7741 gctggtaaat atcttctaca aattgttttt cttcctctgt tgtcacctcc tgctggaggg
     7801 caaaaataaa gtctgtaaaa gatagatttt tcagactacg atagacaaaa gagaggcttt
     7861 ccgcttcctc tccttgtctt atcaactctt cttcaaaatt tgaaaataat tgagctaatt
     7921 tcattatttg tttaattctt ctagtttttg tgtttggtca taaagcacca aggcatccac
     7981 aacttcgtcc aatttaccag acaaaatcgt atctagtttt tggagggtca agccgatacg
     8041 gtggtctgtg acacggtttt gtgggaagtt ataagttcgg atccgttctg aacggtcacc
     8101 agtaccgatt gtcgacttac gctcagcgtc ttgttcatcc tgagcaatct gagcaaagtg
     8161 gtcagcgacg cgtgcacgga taatcttcat agccttctcg cggttcttct gctgggtacg
     8221 ttcttcctgc atctcaacct tgatattggt tggcaagtga acgatacgaa cggcagtcgc
     8281 aaccttattg acgttctgtc caccagcacc agaggcgtga tagatgtcga cacgaaggtc
     8341 ttttggatca atgtcgtatt caacctcttc aacttctggc ataacaagaa ctgtcgctgt
     8401 cgaagtatga acacggcctt ggctttctgt cacaggaaca cgttgcacac ggtgggcacc
     8461 tgattcatac ttaagcttag agtatacaga ctgacctgaa accatagcaa ccacttcttt
     8521 aaaaccaccg acaccattca tagaggcttc catgacttca aagcgccaac cttgggcttc
     8581 cgcatacttt tggtacatag ttagcaaatc tccagcgaaa agtgccgctt cgtctccacc
     8641 agctgctcca cggatttcaa ggatgatatt cttgtcatcg tttggatcct ttggaaggag
     8701 caaaattttc agtttttctt catattcttc tttttcagcc ttggcatctt tgagttcttg
     8761 cttggccatt tcttccaagt ccgcatctcc gcctgattcc ttaatcatct cttcggcatc
     8821 gacgatattt tgaaggactt gtttatactc acggtaggct attacggtgt cacgattgga
     8881 agcttcttct tttgaaagct ccataaaacg cttggtgtct gaaacgacat cagggtcact
     8941 cagcaattct cctagttctt cataacggtc ttctacagct tgtagttgat catagatgtt
     9001 catttttctt cattctcctt attgatttca ggggcaaaat aatgtttacg gcaaaccgag
     9061 atataggttt cattaccacc aatctggatc tgttctccat cgtaaacggg cagtccatcc
     9121 tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt cttgatttcg
     9181 tcaatcttgt ctgctaaaag caagagatat ttggaacctt cgaacaattc attgcgaaag
     9241 tcatttttca agccaaaagc catgacgggt atgtctaact cgtccacaac acgagctagg
     9301 tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaaaacaca gtaaggtttt
     9361 tctggtaggt ctcggatata gccaaagata tccgttgttt cctcaatcgc aagggcaggg
     9421 cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc cagagccgag
     9481 gtcataatca caacaccttt tccttgctcc tcgtagttat aggccacttt gagaatctca
     9541 atcgttttac cagagttcat ggtcccataa cgatagtaca actgtgccat gtttcttgct
     9601 tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct taagctttaa
     9661 acggcaaaat gtggtaaaat ggaagaaatc aaaactagtg gaggaagcta ttatgccatt
     9721 tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc ttgctaagga
     9781 agtaacggaa gcggttgttc gcaacactgg agcacctcaa tctgctgttc atgtcatcat
     9841 caacgacatg ccagaaggaa cttatttccc acaaggggaa atgcgcacca aataagctag
     9901 cttaagcaga attgcttagg ctttttcaat ctccaagtag cacccattga agaaatagtc
     9961 taaatttgtt acaatttgaa aagagacttg gaaaattcca agaaaagagc aattaattaa
    10021 aggaaacatt atgattacac gtgaatttga taccatcgct gctatctcta ctccactagg
    10081 tgaaggggct attggtattg tccgcctgag cggaacagac agttttgcta ttgtgcaaaa
    10141 gatttttaaa ggaaaagact tgaacaaggt tgccagccac actctcaact acggtcacat
    10201 tattgatcct ctgactggta aagtcatgga cgaggttatg gttggggcta tgaagtctcc
    10261 aaagaccttc actcgtgagg atattatcga gattaacacc cacggtggga ttgcggtgac
    10321 caatgaaatt ctccagctag ctattcgtga aggggctcgg ttggcagaac ctggtgaatt
    10381 taccaaacgt gcttttttaa acggtcgcgt agacttgaca caggcagagg ctgtgatgga
    10441 tatcatccgt gccaagactg acaaggccat gaacattgcg gtcaaacaat tagacggctc
    10501 cctttctgac ctcattaaca atacccgtca agaaatcctc aatacacttg cccaagttga
    10561 ggtcaatatc gactatcctg aatatgatga tgttgaggaa gctactactg ccgttgtccg
    10621 tgagaagact atggagtttg agcaattgct aaccaatctt cttagaacag cccgtcgcgg
    10681 caaaatcctt cgtgaaggaa tttcaacagc tatcatcgga cgtcccaacg ttgggaaatc
    10741 cagccttctc aacaacctct tgcgtgagga caaggctatc gtaacagata tcgctgggac
    10801 aacacgagat gtcatcgaag agtacgtcaa catcaatggt gtacctctca aattgattga
    10861 tacagccggt attcgtgaaa cagatgacat agtggaacaa atcggtgttg agcgttcgaa
    10921 aaaagccctc aaggaagccg acttggttct actagtgcta aatgccagtg aacaactgac
    10981 tgcgcaagac agacaacttc ttgaaattag ccaagatacc aatcgcatta ttctacttaa
    11041 taaaaccgac ctgccagaaa cgattgaaac ttcgaaacta cctgaagacg ttatccgtat
    11101 ttcagtcctt aaaaaccaaa acatcgacaa gattgaagag cgaatcaaca acctcttctt
    11161 tgaaaatgct ggcttggtcg agcaagatgc tacttacttg tcaaacgccc gtcacatttc
    11221 cctgattgaa aaagcagttg aaagcctaca agccgttaat caaggtcttg agctggggat
    11281 gccagttgat ttgcttcaag ttgacttgac tcgtacttgg gaaatcctcg gagaaatcac
    11341 tggggatgct gctccagatg aactcatcac ccaactcttt agccaattct gtttaggaaa
    11401 ataagaaaaa tccatgatcc ttcattcggt catggatttt attgtcttta ttagtaatct
    11461 ggtcttaaga cccctgttac agttgcctta gttgcttcgt agtcgccatc tacgacaacc
    11521 ttgataatgc gtttggcatc ttcttctggt gctggaacaa gaggtagacg agtgggtcca
    11581 gcttcaaatc ccatatagtt aagaattgcc ttaactggag caggacttgg ataagagaag
    11641 agagcattaa ccttaggaat gaatttacgc tgaattgctg cggctttctt catatcgctt
    11701 tctgcaatgg cagtaaacat ctcgtgcatt tcatccccat ttgtatgaga ggcaacagaa
    11761 ataaccccat ccgccccaag gttcatggca tggaaagcat ctccatcctc acctgtataa
    11821 atcaagaact cttcaggctt gtgctcaatc aagtaagcca tattagccaa gctagtacat
    11881 tctttgacac cgataatatt tggatggtca gccaagcgaa gcatggtttc tggagtcaat
    11941 tcgacaacta cacgccctgg aatgttatag ataataattg gtaggtcaga agcatctgca
    12001 atagccttaa agtgctgata catcccttct tgagaaggtt tgttgtagta aggaacaata
    12061 gcaagcccag ctgcgaaacc accaaattcc gctacttctt tgacaaactc aatagagtca
    12121 cgcgtatcat tagtacctac acccgcaatc aaaggaacgc gtccattgac aaccttttgt
    12181 acagccgcaa acaactccaa ctcctcatcg tgggtcaaag ttggactctc agcagtcgtt
    12241 cctgcgagaa gaattccatc cgtatgatgg gccaataaat gctcaatcaa ggctggaata
    12301 gcatcaaagt taatggaacc atcctcatgg aagggggtaa taaaggctgt aatgatttta
    12361 cattctttta aatcttgata agacacgaaa acacctctct atttcaaaga aggagttcat
    12421 ctgaactcct aaacgatatg actattttaa ttcaaatttc aattcagctg ttggacgaac
    12481 caatccacgt tcatgaagag tttcagcaat ctgaactgag ttccaagcag cacctttgag
    12541 aaggttatct gaaacaaccc acatgtgaat tcctttttct gcatccaagt ctttacggat
    12601 acgaccaaca aaggtatcac gtgaaccaac tgcattgatg gcttgaggat agatttgatg
    12661 aactacatca tcttcaagaa cagcacctgg gaaggatgcg atagctgctt tgacttcttc
    12721 aattggagcc acttcctttg tttcaatata aactgactca gagtgagctg acaagactgg
    12781 aatacgcaca catgtcgcag atactgcaat gctatcatct tccataattt tcttagtttc
    12841 cttggtcatc ttcatctctt cgtacgtgta atcattatca gtgaagacat caatttgtgg
    12901 aagagcgtta aaggcgatag gataatgttt cttgtcacca cctgaaggca agatttccgc
    12961 atgcaaatca cgtggtttca ctccatcatt caagacctca cgaagttcac gttgtgtctc
    13021 aagaattgct cccataccag cacctgaaac agcttgataa gttgaaacaa tgatacggtc
    13081 caagccccat ttttggcgaa ccggctcaag agccaccatc atttggattg ttgaacagtt
    13141 agggcaggca atgattccgt tgtgggcatc aagtgcatga gcattgacct ctggaacaac
    13201 caaaggaaca tctggatttt gacggaaata agatgtatta tccactacta ccacgccagc
    13261 ttttactgcg tatggtgcat acttagctga tgtcgaacca cctgctgaaa agagagcaat
    13321 atcaactcct tcaaaagctg tttcagtcgt ttcttcaatc gtaatatctt gatctttaaa
    13381 tttcaatgac ttacctgctg aacgtgcaga agcaaggtaa cggattttat cgattggaag
    13441 tgttgattct tccaacattt ttatcatctg agcaccgaca gcacctgtcg cgccgactac
    13501 agcaactgta tatcccataa ataacctctt tcggaatttt ctaaaaattt ctataataga
    13561 agtattatac tactttttcc atgaattgca aagctttttc atcatttttt ggaaaataaa
    13621 aatccccaat cgctagacta gggattaaga ggcatcttca tgtgatgagc aggttcacac
    13681 aactcatcaa ggtccgctcc tgcgttatga cctccttatg ctcaatagta gcccgaagac
    13741 tacctatcga ctcatcgcat ctactattct actagataga gtcatttttg tcaacagcca
    13801 aaactcttta tactcttcga aaatctcttc aaacttcgtc agcgtcgcct tgccgtatgt
    13861 atggttactg acttcgtcag tttcatctac aacctcaaaa acatgttttg agctgacttc
    13921 gtcagtttca tctacaacct caaaaacatg ttttaagctg acttcgtcag ttctatctac
    13981 aacctcaaag cagtgctttg agcaacctgc ggctagtttc ctagtttact ctttgatttt
    14041 cattgagtat tagttttatt tacacaggtg cataagaaat atattggaaa aattgggggc
    14101 agacataaca aaaacccttg taaatcaagg gcttttctgt ctatttaatg ctaattgccg
    14161 ggattgaacc ggcgacctca tccttaccat ggatgcgctc tgccaactga gctaaatcag
    14221 cttacttaag aagtatacta tattcagaaa aacttgtcaa gtttccttag aaattttcca
    14281 taggaaaaag ccaggtaaga actacctagc ttatcttctt ctatttactt aaatcaattc
    14341 gacgaccaat tttaccaata ataatcgctc caataatcaa tgaagcaaat tcaccgattc
    14401 ctgttgtaaa ccaagtaagg aagaatggta attctgctac aatatttaac tctgcagcga
    14461 tagtaaacat ggaaattgaa aagaggattg aaaagaagaa atgatctttt cgaatcaatc
    14521 cgttaaagag gtaatccttg ctatattttg caaaaagcca aacacctaaa ctgaggaata
    14581 ctaaggttga accaccacca acaaagacat ctagcagtcc gaagctaaag aaattagcaa
    14641 tcatacaacc gatggtaact ccgatgatgt acttaggatt gtaaaaagcc ataaagttca
    14701 tcatttctga aatacgaaac tgataagcac catagctgat ggcatttagg ggcggtgtga
    14761 cagtcaaaac gacgtagata gcagcgacga ttgcaatatc tgcaacatca cgaatagtta
    14821 attttttcat cttttctcct ttggcggtta tccgcgtgta atatgcttgg tgaaagaagc
    14881 taagcaccaa gggttgattc attcaacctt actagtatag cacaatagcc tgctttatgc
    14941 tatacttaaa gcatgaaaaa acctattcaa aaatttttta acaatgaaat cttagcctat
    15001 ctcttttttg gccttgcgac aactctagtt tcgattctct cacgactagt catttatcaa
    15061 ctaagtcatc aggaactcct tgctacggcc ttggcaaata ttatcggtat cttatttgcc
    15121 tttatcacaa atgatacgat tgtatttaag caaaaaaaga aaaatagact tatccgttta
    15181 gtgaaatttt ctcttgctcg cctttctact tttctgttag acttgttttt aacttttctc
    15241 tttgtaaccc aatttcctca catcataggg caattcgtaa ctgaaaacat tgataagata
    15301 aatgctattg aaacaataat tgcacaaata ttcattatta tcatcaatta tatcttgagc
    15361 aaggtctata tttttagaaa ataatgctcc atttatgagc cttttaattg ccttctataa
    15421 tgatttagga tacaatgaaa cacgaaacat tgaaaggaaa acagtatgtt aaaaaatcta
    15481 aaatcgttct tgcttcgagg aaatgttatt gaccttgctg tcggtgttgt cattgcctct
    15541 gcttttggtg ctatcgttac ttcacttgta aacgacatta tcactcctct tattttaaat
    15601 cccgctttga aagctgctaa agtcgaacgt atcgctcaac tttcttggca tggagtcggc
    15661 tatggtaact ttttaagtgc tattatcaat tttatctttg tgggtaccgc cctcttcttt
    15721 attatcaagg gcattgaaaa agcacagaaa ctgactggca taaagaaaga aaaaactgcc
    15781 gaaaaaaaac caaccgaatt ggaagtcctt caagaaataa aagctctcct tgagaaaaaa
    15841 taatcgatta aaaggatcta gcgcaaagct aaatcctttt ttctttactc tttgggtaac
    15901 ttgcctgctt tttcaagcat ttttttgata agataaggca tcttaacatt ctcccgccct
    15961 tttttctcaa tcagtttttt cacaaattct ggcatttgta aatcttcaga ttcagctaaa
    16021 atgtttttcg tctcgtctga aggaactaac tcatcaaagg tctcttcttc tgggagaaat
    16081 tccttaaact cttgattttc attgactcta actgtattga ccaaagcatc aatcaaacga
    16141 gaatctgtat ttggcattgg tggacgatgg tagtttaccc caaattcctg gcataagtca
    16201 taacattcta catcattgtc aaacaagacc tcaatatgct cactgatgaa actaataggt
    16261 acaaaaatat aatggtctgg atgttctgtc tgttctctga gatactccaa aacatctggt
    16321 ttaatccatg gaataccaat atcactctca ctctgccaag tgttggtata ttgttctgaa
    16381 ctcaagccca gtttttcagc gactagcttg ctattttcga aaatttggtc tatataagga
    16441 tcaccaaagt ccaaggcaaa aatgggcaca ctatgggctg aaaagatgac tttaaagctg
    16501 tcttgcttta cttcttcttt taaaatctta gcaatttcat ctgcccaata gtttaagagc
    16561 gcttcttcct gataccagtc cttaatgacc aaaaattgaa tttgtttgct ttctaaaaat
    16621 ttttcatagc ccatgacaga gtagaaagaa taatggggct ccaaaatcaa gcaaatacac
    16681 tgttcaattc cgtctgcttc catttgaccg atcacatctg gaataaaggg actggaaaac
    16741 ttattagcaa agtagacact gtattcattc cccagcctag cttctaccaa ggcaacctct
    16801 tgacgggtaa ttttttgcag aggcgtgcct ccaattcgta cataattatc atagagagtc
    16861 tgaatctcgt ggtcttgggg tctcacccca cgacgaatat ttgtgaaaaa atcagctaca
    16921 ccttcaaagg taatctcttc tggtgaaccg aaggtcatca ttaaaattgc ttttttcatg
    16981 actgcttcct tgtgatgttt ttatcaagtt tattttatca ttaattaatc gataagtaaa
    17041 cgctaatata gggacattct caatcttctg tcttttgttt ttctcttctt tctatgatac
    17101 aatggaaaaa ataaattcaa aaggagtttt tttatgactt atccaaatct cttggaccgc
    17161 ttcttaacct acgttaaggt caacacgcgc tctgatgaac actctactac tactccaagt
    17221 acacagagtc aggttgactt cgcaacaaat gtcctaattc ctgaaatgaa acgtgttgga
    17281 ctgcaaaatg tttactatct accgaatggt tttgctattg gaaccttgcc agccaacgat
    17341 ccgtctttaa cacgtaagat tggttttata tcgcacatgg atactgctga ttttaatgct
    17401 gaaggagtca atccacaggt aattgaaaac tacgatggtg gtgtgattga actagggaat
    17461 tctggtttca aactcgatcc agctgacttc aagagtcttg aaaaatatcc aggacaaacg
    17521 ctcatcacaa cagatggaac aagcttgcta ggtgctgatg acaagtcagg aattgctgaa
    17581 attatgacag ccattgaata tctaactgct catcctgaaa ttaagcactg tgagattcgt
    17641 gttggttttg gtccagatga agaaatcggt gttggtgcca ataaatttga tgcagaagat
    17701 tttgatgtgg attttgccta cactgttgat ggtggtccac taggtgaact tcagtacgag
    17761 actttctcag ccgctggtgc tgaattgcat ttccaaggcc gtaatgtcca ccctggtact
    17821 gccaaagagc agatggtcaa tgcccttcag ctagcaattg attttcataa tcaacttcca
    17881 gaaaatgacc gacctgagtt aactgaaggt taccaaggtt tttaccatct aatggatgtg
    17941 acaggtagtg ttgaggaggt gcgtgcaagc tacatcattc gtgattttga aaaagatgcc
    18001 tttgaagcgc gtaaagcatc catgcaatct atcgctgata agatgaatgc agaacttggg
    18061 agctaccgtg tcactctcaa cttgacagac cagtactaca atatgaaaga agtcattgaa
    18121 aaagatatga ctccaattac cattgctaaa gccgttatgg aagatctagg tatcacgcct
    18181 attatcgaac caatccgggg tggaacagac ggctctaaga tttcctttat gggaatccca
    18241 actccgaata tctttgcagg tggcgaaaat atgcacggac gttttgaata cgttagcctt
    18301 cagactatgg aacgtgcagt tgataccatc attggcatcg tagcttataa aggctaaaaa
    18361 gacgaggtag ctcagctact tcgcctttct ttttattcta ctggtttttc ttgatttcca
    18421 gtagttgtag aagattctgt tgtttcattt tctgaagttg attcagcagg tttagaatct
    18481 cttgtattgc ttggtttgtt ttcgtcgcta gcaatttcaa tgttagattc tgcagttgcg
    18541 tttggttggt tctcagcact ggtgttatca ctatttgctt cagcatttct tgctggactt
    18601 gtttcttcac ttgcgctagc ttttgactgg atttgatgat tcaaaactag aatagctttt
    18661 gtcgattcaa gtaaagctgt tttgtcttta ctcttagcag aaagttgatc taataatgca
    18721 tccaccttat caaagtccgc atcagatcca ttattacttt ctaaataaga gtgaagcgac
    18781 atgagaatat cgtagagttt ttgatagagt acaagtgtct gagaatcttg ctcagcattt
    18841 tccttttctt gttgaagggc gctagcgata cgagtcaaga catcttttac ctgactgttt
    18901 acttcatcca agtctgcatc agccttgttt gtggcagctt ttagattttc tacttcttct
    18961 gccaaggatt gtctgattcc ttcttcatgg atttgttcca agagttgatt taccttgctc
    19021 aaaagacttt ctacttcttc cttgctatct gtcgcagatt attggttgcc atctaccatg
    19081 tactcctaaa acaggagagt tataatccaa gattacaagg ccttacagaa ataagaaatc
    19141 cagataagac aatgttcgtc caagacgcta ttcgcttcgc acagcagcac ggattcaata
    19201 tgctttaatt ttaaagttta ggtgtcaaga cctcttttta gtgtgcccaa aatttagaga
    19261 agtaatcaat caactaactt ttattttttt caaactttca gtaaactgac ctaaagctaa
    19321 ctcaatctgt ctttgtagat gcttctgcta tcagctagaa gttgatctac ttttgccaag
    19381 actgccttct catcaaaagt tccaggttga tagttggatt gcagggatgg aatcttgttt
    19441 ttcaaagccg cttcatatcc cttagtttga accttgatgt agtgattgtg gtcgccatga
    19501 ggaatcacaa aaccttctga atcttcactt ataattcgat tggcatcaaa accatgacca
    19561 tcttcttcct catgatggac atgtagtgac ggattactta atacagaact agaagaactt
    19621 cctacctctt ccgtgttaga gtgtgatggg ggattgttaa gagatgactt aggaatatag
    19681 tgatagtgat ccccatgtct tactatataa gcatcacctg tatctctgac aatatcatta
    19741 gggttaaaga catatgtggc tgctaattca cctgccgaca agtcactctc aggaatgaaa
    19801 tgatagtgac caccatgtgg tactatagta gattgaaata gaatatgagc aaattgataa
    19861 ggggatttta aagtaatttc taacaatgat ttagaaacta tgatgtgcta ttctaaattc
    19921 aactcactat atataaccat catcggtagt ataacgtccc tgtaattttg ctacagatac
    19981 ttctgcacta gctcctttat cgtctttacc atgttcttgt ttttggcgat tgatttcatc
    20041 ttttgttcgt acattttctg catgagcttg atctttaagg taaacataat actttccatc
    20101 taccttaata atatatcctc ccttaaccta actgacgata tcttgatctt tcggctgata
    20161 gttgggggct ttcattaata gctcttcact aaagagcgca tcaaaaggaa ctttaccatt
    20221 atagtagtga taatgatcgc catgagaagt tacataacct tgatctgtaa tcttaataac
    20281 aatttgtttt gcttgaattc cttctttttg actaacctag tctggagtca aattttcagt
    20341 cttcttagtg tctttattac tgtttacata tgaaacacga tttttatctg tattggcctg
    20401 ttagctatgt tggttcagag cataaacaca cagacttaag gaaaggataa caac
//
LOCUS       DAVKFH010000028        20025 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKFH010000028 DAVKFH010000000
VERSION     DAVKFH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.028"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            51..2231
                     /locus_tag="VW006_001221"
     CDS             51..2231
                     /locus_tag="VW006_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9380078.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2283..3098
                     /locus_tag="VW006_001222"
     CDS             2283..3098
                     /locus_tag="VW006_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9380079.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            3238..4581
                     /locus_tag="VW006_001223"
     CDS             3238..4581
                     /locus_tag="VW006_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9380080.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            4797..5987
                     /locus_tag="VW006_001224"
     CDS             4797..5987
                     /locus_tag="VW006_001224"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9380081.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSEEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIKK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6540..7475
                     /locus_tag="VW006_001225"
     CDS             6540..7475
                     /locus_tag="VW006_001225"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9380082.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7509..8546
                     /locus_tag="VW006_001226"
     CDS             7509..8546
                     /locus_tag="VW006_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9380083.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8719..9324
                     /locus_tag="VW006_001227"
     CDS             8719..9324
                     /locus_tag="VW006_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9380084.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELTAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9481..10011
                     /locus_tag="VW006_001228"
     CDS             9481..10011
                     /locus_tag="VW006_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9380085.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10032..10934
                     /locus_tag="VW006_001229"
     CDS             10032..10934
                     /locus_tag="VW006_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEU9380086.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVSCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            10934..11416
                     /locus_tag="VW006_001230"
     CDS             10934..11416
                     /locus_tag="VW006_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9380087.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQPL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            11418..12959
                     /locus_tag="VW006_001231"
     CDS             11418..12959
                     /locus_tag="VW006_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9380088.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(13015..13818)
                     /locus_tag="VW006_001232"
     CDS             complement(13015..13818)
                     /locus_tag="VW006_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9380089.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(13902..14115)
                     /locus_tag="VW006_001233"
                     /pseudo
     CDS             complement(13902..14115)
                     /locus_tag="VW006_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14594..14803)
                     /locus_tag="VW006_001234"
     CDS             complement(14594..14803)
                     /locus_tag="VW006_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380090.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            14973..15245
                     /gene="rpsP"
                     /locus_tag="VW006_001235"
     CDS             14973..15245
                     /gene="rpsP"
                     /locus_tag="VW006_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9380091.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            15265..15504
                     /locus_tag="VW006_001236"
     CDS             15265..15504
                     /locus_tag="VW006_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9380092.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            16081..16869
                     /locus_tag="VW006_001237"
     CDS             16081..16869
                     /locus_tag="VW006_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9380093.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPKMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            16900..17418
                     /gene="rimM"
                     /locus_tag="VW006_001238"
     CDS             16900..17418
                     /gene="rimM"
                     /locus_tag="VW006_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9380094.1"
                     /translation="MNYFNIGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            17408..18127
                     /gene="trmD"
                     /locus_tag="VW006_001239"
     CDS             17408..18127
                     /gene="trmD"
                     /locus_tag="VW006_001239"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9380095.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDDPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            18139..18477
                     /locus_tag="VW006_001240"
     CDS             18139..18477
                     /locus_tag="VW006_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380096.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(18508..18828)
                     /locus_tag="VW006_001241"
     CDS             complement(18508..18828)
                     /locus_tag="VW006_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380097.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            18926..19141
                     /locus_tag="VW006_001242"
     CDS             18926..19141
                     /locus_tag="VW006_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9380098.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(19210..19296)
                     /locus_tag="VW006_001243"
                     /pseudo
     CDS             complement(19210..19296)
                     /locus_tag="VW006_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(19347..19883)
                     /locus_tag="VW006_001244"
     CDS             complement(19347..19883)
                     /locus_tag="VW006_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9380099.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT         6168 a         3639 c         4331 g         5887 t
ORIGIN      
        1 gcaaacgttt tcgtaaaacg tttgcctaaa taaaaataaa ggagatttga atgatgaaag
       61 atacattcaa aaatgtcttg tctttcgaat tttggcaaaa attcggtaag gctttgatgg
      121 tagttatcgc ggttatgccg gctgctggtt tgatgatttc aatcggtaag tctatcgtga
      181 tgattaaccc aacctttgca ccacttgtca tcacaggtgg aattcttgag caaatcggtt
      241 ggggggttat cggtaacctt cacattttgt ttgccctagc cattggagga agctgggcta
      301 aagaacgtgc tggtggtgct ttcgccgctg gtcttgcctt catcttgatt aaccgtatca
      361 ctggtacaat ctttggtgta tcaggcgata tgttgaaaaa tccagatgct atggtaacta
      421 ctttctttgg tggttcaatc aaagttgctg attactttat cagtgttctt gaagctccag
      481 ccttgaacat gggggtattc gtagggatta tctcaggttt tgtaggggca actgcttaca
      541 acaaatacta caacttccgt aaacttcctg atgcactttc attcttcaac gggaaacgtt
      601 tcgtaccatt tgtagttatt cttcgttcag caatcgctgc aattctactt gctgctttct
      661 ggccagtagt tcaaacaggt atcaataact tcggtatctg gattgccaac tcacaagaaa
      721 ctgctccaat tcttgcacca ttcttgtatg gtactttgga acgtttgctc ttgccatttg
      781 gtcttcacca catgttgact atcccaatga actacacagc tcttggtggt acttatgaca
      841 ttttaactgg tgcagctaaa ggtactcaag tattcggtca agacccacta tggcttgcat
      901 gggtaacaga ccttgtaaac cttaaaggta ctgatgctag tcaatatcaa cacttgttag
      961 atacagtaca tccagctcgt ttcaaagttg gacaaatgat cggttcattc ggtatcttga
     1021 tgggtgtgat tgttgctatc taccgtaatg ttgatgctga caagaaacat aaatacaaag
     1081 gtatgatgat tgcaacagct cttgcaacat tcttgacagg ggttactgaa ccaatcgaat
     1141 acatgttcat gttcatcgca acacctatgt atcttgttta ctcacttgtt caaggtgctg
     1201 ccttcgctat ggctgacgtc gtaaacctac gtatgcactc attcggttca atcgagttct
     1261 tgactcgtac acctattgca atcagtgctg gtattggtat ggatatcgtt aacttcgttt
     1321 gggtaactgt tctctttgct gtaatcatgt actttatcgc aaacttcatg attcaaaaat
     1381 tcaactacgc aactccaggg cgcaacggaa actacgaaac tgctgaaggt tcagaagaaa
     1441 ccagcagcga agtgaaagtt gcagcaggct ctcaagctgt aaacattatc aaccttcttg
     1501 gtggacgtgt aaacatcgtt gatgttgatg catgtatgac tcgtcttcgt gtaactgtta
     1561 aagatgcaga taaagtagga aatgcagagc aatggaaagc agaaggagct atgggtcttg
     1621 tgatgaaagg acaaggggtt caagctatct acggtccaaa agctgacatt ttgaaatctg
     1681 atatccaaga tatccttgat tcaggtgaaa tcattcctga aactcttcca agccaaatga
     1741 ctgaagcaca acaaaacact gttcacttca aagatcttac tgaggaagtt tactcagtag
     1801 cagacggtca agttgttgct ttggaacaag taaaggatcc agtatttgct caaaaaatga
     1861 tgggtgatgg atttgcagta gaacctgcaa atggaaacat tgtatctcca gtttcaggta
     1921 ctgtgtcaag catcttccca acaaaacatg cttttggtat tgtgacggaa gcaggtcttg
     1981 aagtattggt tcacattggt ttggacacag taagtcttga aggtaaacca tttacagttc
     2041 atgttgctga aggacaaaaa gttgcagcag gagatctcct tgtcacagct gacttggatg
     2101 ctatccgtgc agcaggacgt gaaacttcaa cagtagttgt cttcacaaat ggtgatgcaa
     2161 ttaaatcagt taaattagaa aaaacaggtt ctcttgcagc taaaacagca gttgctaaag
     2221 tagaattgta atatacttga ggttggaagc tgtattccaa cctcttattt tgggagaaaa
     2281 gaatgaaatt tttaacactc aatactcaca gttggatgga gaaagaagca gaggaaaaat
     2341 tccagatttt gcttgaagat attcttgaaa aggactatga tttgatttgt ttccaagaaa
     2401 tcaatcagga gatgacctcg tcagaggtgg aggttaatga cctttatcaa gctttgccag
     2461 cagctgagcc tattcaccaa gaccattatg ttagactctt ggttgaaaag ttgttagagc
     2521 aagggaaaaa ttactactgg acctgggctt ataaccatat cggctatgac cgctaccacg
     2581 aaggtgtggc tatcttgtct aaaacaccta ttgaagccag agaaattttg gtttcagatg
     2641 tggatgatcc aacagactat catactcgcc gtgttgccct agctgaaact gtagtcgatg
     2701 gcaaggagct agcagttgcc agtgttcatc tctcttggtg ggataaaggt ttccaagaag
     2761 aatgggcacg atttgaggct gtcttgaaaa aattgaacaa gccactttta ctagctggag
     2821 attttaacaa cccagcaggt caggaaggtt accaagctat tttagctagt ccattaggct
     2881 tacaagacgc atttgaagtt gctcaagaga aaagtggtag ctatactgtt ccgcctgaaa
     2941 ttgatggctg gaaagggaac actgaacccc ttcgaatcga ttatgtcttt actaccaaag
     3001 agttagcggt ggaaaattta catgtcgtat ttgatggtaa caagagtcca caagtgagtg
     3061 atcactatgg cttgaatgct atattaaact ggaaataata actgaaaaga ggttggaact
     3121 ataaaattcc agccttttct tactagagaa gctactggaa atagcctaaa taagtgagac
     3181 tactgtaatg gaataaaata tggtataatt gataaggtag atagaatcga ggatgttatg
     3241 tcatttacga aatttcaatt taaaaactat attagagaag ccttgaagga gttaaaattt
     3301 acaactccaa cagaggtgca agacaagttg attcctattg ttttggcagg tcgtgaccta
     3361 gtaggagaat caaaaacagg ttcaggtaag actcatactt tcttgttacc gattttccag
     3421 caattagatg aagctagcga tagtgtacaa gcagtgatta ctgcaccgag tcgtgagttg
     3481 gctactcaaa tttaccaagt agcgcgtcag atttcagctc actcagatgt cgaagttcgt
     3541 gtggttaatt atgtgggtgg tacggataag gctcgccaga ttgagaaatt ggcaagcaat
     3601 cagcctcata ttgttattgg aacaccaggc cgtatctacg acttggttaa atctggtgat
     3661 ttagctattc ataaagccaa gacatttgtt gttgatgaag cagatatgac cttggatatg
     3721 ggattcttgg aaactgttga taagattgct ggcagtcttc caaaagactt gcaattcatg
     3781 gtcttctcag cgactatccc acaaaaactg caaccattct tgaaaaaata cttatcaaat
     3841 cctgttatgg agaaaattaa gaccaaaacg gttatttctg acaccattga taattggttg
     3901 atttcgacca agggacatga taagaatgct caaatttacc agttgactca gttgatgcag
     3961 ccgtatttgg caatgatttt tgttaacact aaaacacgtg ctgatgaatt gcattcatat
     4021 ctgactgctc aaggcttgaa ggttgcaaaa atccatggag atattgcccc tcgtgaacgc
     4081 aagcgaatca tgaatcaggt gcaaaatctg gattttgagt atattgtcgc aacagatttg
     4141 gcagcgcgtg ggattgacat tgaaggtgtc agccatgtca tcaatgatgc cattccgcaa
     4201 gacttatctt tttttgttca tcgtgttggt cgtactggac gaaatggcct accaggtaca
     4261 gctattaccc tttatcagcc aagtgatgac tcggatatcc gtgagttgga gaaattggga
     4321 atcaagttta gtcctaagat ggtcaaagac ggggaatttc aagataccta tgaccgtgat
     4381 cgtcgtgcca accgtgagaa aaaacaagat aaacttgata tcgaaatgat tggtttggtt
     4441 aaaaagaaaa agaaaaaagt caaaccgggt tataagaaga aaattcaatg ggcggttgat
     4501 gaaaagcgcc gtaaaaccaa gcgtgctgaa aatcgcgctc gcggtcgtgc agagcgtaaa
     4561 gctaaacgcc aaacatttta atagaaattg ttggagtatt gagctccaac ttttttattt
     4621 atgagaacga actatctaaa ccgaaacact acattaaaga ctgcaaattg cgattaaaaa
     4681 tggtataatg ataaagttat atagtcccga taagatggta ggtatttatt acgaagagtt
     4741 ttcctatcag tactttgtaa ctctataaca atatttttta agggggggac atttttatgt
     4801 cagagcgtaa attattcacg tctgaatctg tatctgaggg gcatccggat aagattgcag
     4861 accaaatttc agatgcgatt ttggatgcta ttttagcaaa ggatccagag gcgcacgttg
     4921 ctgctgaaac agctgtatat actggttctg tccacgtttt tggtgaaatt tctacaaatg
     4981 cctatgtgga tattaaccgt gtggttcgtg ataccattgc agagattggt tataccaata
     5041 cagaatatgg attttctgct gagacggtgg gagtacaccc atctttggtg gaacaatctc
     5101 ctgacatcgc tcaaggtgtt aacgaagcct tggaggttcg tggaaatgct gatcaagatc
     5161 cactggactt gattggagca ggtgaccaag ggctcatgtt tggatttgca gtagatgaaa
     5221 cagaagagct catgccattg ccaattgcac tcagtcataa attggttcgt cgtctggcag
     5281 aacttcgtaa gtctgaagaa attagctatc tccgtccaga tgcaaaatca caagttacag
     5341 ttgagtacga tgaaaatgac cgtccggtac gtgtagatac agtcgttatt tctactcagc
     5401 atgatccaga ggccactaat gaacaaatcc atcaagatgt gattaaaaag gtcatcaaag
     5461 aagttattcc atcttcttat cttgatgata agacaaaatt ctttatcaat ccgacaggtc
     5521 gttttgtaat cggtggtcct caaggggact caggtttgac tggtcgtaag attattgtag
     5581 atacttatgg tggctactct cgtcatggtg gtggtgcctt ctctggtaaa gatgcgacta
     5641 aggtggatcg ttcagcctct tatgcggctc gctatattgc caagaatatc gttgcagcag
     5701 accttgctaa gaaggcagaa gtgcagttgg cctatgctat cggtgttgcg caacctgttt
     5761 ctgttcgtat cgatactttc ggtacaggaa cagtagctga aagtcaactt gaaaaagcgg
     5821 ctcgtcaaat ctttgacctt cgccctgcag ggattatcca aatgctggac ctcaagcgtc
     5881 caatttaccg tcaaacatcg gcttacggtc acatgggacg tacagatatt gatcttccat
     5941 gggaacgttt ggataaggta gatgctttga aagaagcagt aaaataagat tttaagaggg
     6001 gaacgtcctc tcttttttat agtttttaac tatactggga tactgttctg aaaatccatt
     6061 ttgtgaaagt agagatttac atgtatagta gattgaaact agaatagtac acctcaactt
     6121 ctaaaacatt gttagaaatc gatttgactg tcctgattga tttctcctgt tcttgtttca
     6181 ttttactata tttctttaaa aatgataaag gttgagattt ctcctcgtaa tagataatca
     6241 tggggatatt tcaatccaaa gttttattcg ttatcacttg actattgcaa ggttttctag
     6301 agcaacagag tcatggaatg gactcatggt tgagatttct ccttgttgct tggacttcat
     6361 tcaaaagtct gttacccaag ccttgttcaa acttccaata cactagctgt ttccatagca
     6421 tgacttctgt actagacttt cttttccgaa taaatagata gaaccacaga atctagtaaa
     6481 cctagaatta aaattatggt ataatattag caataaaaga aatctggagg attagaatca
     6541 tggtatcaac gaaaacacaa attgctggtt ttgagtttga caattgcttg atgaatgcag
     6601 caggtgtggc ttgtatgacg atagaggagt tagaagaggt caaaaactca gcggcaggaa
     6661 cctttgttac taagacagcg accttggact tccgtcaggg gaatcctgag ccacgctacc
     6721 aagatgttcc acttggttcc atcaactcta tgggcttgcc aaataatggc ttagactatt
     6781 atttggatta tcttttagat ttgcaggaaa aagagtcgaa ccgaactttc ttcttatctc
     6841 tggtcggcat gtctccagag gaaacccata ctattttgaa aaaagtccaa gagagtgatt
     6901 ttcgtggtct gactgagcta aatctttcct gtccaaatgt tccaggtaaa cctcagattg
     6961 cctatgattt tgagacaaca gaccggattt tggcagaagt gtttgcttac ttcaccaaac
     7021 ctcttggaat taaattgcca ccttattttg atattgttca ctttgatcaa gcggcagcta
     7081 ttttcaacaa atatccgctc aagtttgtca actgcgttaa ctctatcgga aacggcctct
     7141 atatagaaga cgaatctgtc gttattcggc ctaagaatgg ttttggtgga attggtggag
     7201 aatacatcaa accgactgct ctagccaatg ttcacgcctt ttatcaacgt ttaaatcctc
     7261 aaatccaaat tatcggaaca ggtggcgttc tgactggtcg agatgccttt gaacacatcc
     7321 tctgtggagc aagtatggtg caggtgggaa cgacccttca caaagaaggt gtcagtgctt
     7381 ttgaccgcat taccaatgaa ctgaaagcaa tcatggtgga aaaaggctac gagagcttag
     7441 aagatttccg tgggaaattg cgctatattg actaaattaa atcgaaaaat ctgaagaaag
     7501 gagagacgat gctagccatt gaagaaagtc agaagttgac tttatcaaat ttaccgagcc
     7561 tgagcctatt tacagggaca gatcagggtc agtttgaagt gatgaagagt caagtgttga
     7621 aacagattgg gtatgattct gctgacctca actttgccta ctttgatatg aaagaagtag
     7681 tttacaagga tgtggaactg gagttggtca gccttccttt ctttgcggat gagaaaatcg
     7741 tgatattaga ttattttatg gatatcacga ctgctaagaa acgctttttg acagatgatg
     7801 agcttaagtc atttgaggaa taccttgaca atccttctcc aacaaccaag ttgataatct
     7861 ttgcagaagg aaagctggat agcaaaagac ggttagtcaa attacttaag cgtgatgcca
     7921 aggtcttcga tgcagtagaa gtaaaagaac aagaattgcg ccagtatttc caaaagtgga
     7981 gtcagaaaca aggtctgcag tttaccaatc attcttttga aaatctcctc atcaagtcgg
     8041 ggtttcaatt tagcgaaatc cagaaaaatc ttctcttttt acagtcctat aaggcgaatt
     8101 ctgttatcga ggaagaggat attgttaacg caattcccaa gaccttgcag gacaatattt
     8161 ttgatttaac tcagtttatt ctgactaaaa agatggatca ggcgcgcgat ttggtgagag
     8221 acttgacctt gcaaggggaa gatgaaatca aactgattgc agtcatgctg ggacaatttc
     8281 ggacttttac tcaggtgaag attttggcgg agtctggcca aacagaatcg cagattgcaa
     8341 gtagtttagg tagttatctg ggacgtaacc caaatcctta tcaaatcaag tttgcattaa
     8401 gagattcgag aggactttct ttgagctttc tgaagcaagc tatttcctat ttgattgaga
     8461 cagactatca gattaagaca ggtctttatg aaaaaggttt cctttttgaa aaggcactct
     8521 tacagattgc tagtcaggtc aattgacatt tgttgaaact acaacccacg tcaggattat
     8581 cttgtcgtgg gtttcttgct gatttgctta ttttattaga aagtatgtca gtgatttcct
     8641 tatctataag caaaaaactt gaaaaaagtg aatgtttgcg ttatcatttt catatagaaa
     8701 gaaaaagagg tattacagat ggctattatc ttaccagaac ttccatatgc atacgacgct
     8761 ttggaaccat acatcgatgc ggaaacaatg catttgcacc atgacaaaca ccatcaaact
     8821 tatgtcaaca atgccaatgc agctttagaa aaacaccctg aaatcggtga agaccttgaa
     8881 gccttgcttg ctgatgtaga atctatccca gctgatatcc gtcaagcact tatcaacaat
     8941 ggtggcggac acttgaacca cgctcttttc tgggaattga tgactcccga gaaaacagct
     9001 ccttcagcag aactgacagc agcaatcgat gcaacatttg gttcatttga agaattccaa
     9061 gcagccttca ctgcagcagc aacaactcgt tttggttcag gttgggcatg gttggttgtc
     9121 aacaaagaag ggaaacttga agtgacttca acagcaaacc aagacacacc aatctcagaa
     9181 ggtaaaaaac caatcttggg cttggacgtt tgggaacatg cttactacgt gaaataccgc
     9241 aacgtgcgtc ctgactatat caaagctttc ttttcagtaa tcaactggaa taaagtagat
     9301 gaattgtacg cagctgctaa ataatgatag ttggagggaa gaattgttct tctctttttt
     9361 aggttataag attctggtct gacaaaatcg tcagactttt ttcattttta tgagaaacgt
     9421 gctaactgct agaaattatg gtagaataaa gtattaagta atcgtggaaa aaggatatct
     9481 atgcgaaaag aaattgcacc tgaattatac aactataaca agtttcctgg tccggagttc
     9541 catttacacg gggacaaggt cgaaacggaa gggatagctt tttccttggt tgaaaatatc
     9601 aaggatgcct ttgatgtgac gacttttaat cagcgttttt cagaagtatt aaccaagttt
     9661 gattatatcg tgggggactg gagcaacgaa cagcttcgcc tacgaggttt ttacaaggat
     9721 gaccgaacag aagaaaaact tgaaaaaatc agtcgtttac aagactacct tttagagtat
     9781 tgtagttatg gttgtgccta ttttgtctta gaaaatgaag cccctaagcg agcatcattt
     9841 gacaagaaaa tgcgtaagac ggaagaagaa acaccttcta gaaaaggaaa gaaaccgact
     9901 caaactaaac gaaagtcgaa tgcagataag aaaaatagac gtcgtcagaa agaccaacat
     9961 tctcagaaag aggacaagga acaacgtcat tttgtcattc gtcagaagtg agtagagaat
    10021 aaggagaaga aatgaaaccc tcaattcata gtttggcaca tcaaaccatg caggaatggg
    10081 tattggagca gggagaaaag aaattccgtg cagatcaaat ctgggaatgg ctctaccgta
    10141 aacgtgtgca gtcatttgaa gaaatgacca acctttccaa ggatttgatt gctaagctca
    10201 atgaccagtt tgtggtcaat cccttgaaac aacgtattgt gcaagagtct gctgatggta
    10261 cagtcaaata tctctttgaa ttgcccgacg gtatgttaat tgagactgta ctcatgcgtc
    10321 aacactatgg tttatctgtc tgtgtgacca ctcaggtcag ctgtaatatc ggttgtacct
    10381 tctgtgcctc tggtttgatt aagaagcagc gtgacctcaa taacggtgaa atcgtagcgc
    10441 aaatcatgct ggttcagaaa tactttgctg atgaaggggt tcaggtcaat cttgccgtgt
    10501 ctcttcacgc acccaacaat gaacttcgtt caagtatcat gaaaattaac cgtgcctttc
    10561 cgattgaaaa actctttgct gctattgaat actacattga gacaacaaac cgtcgtgtaa
    10621 cctttgaata tatcatgctt aatgaagtca acgacggtgt agaacaagcc ttggaattgg
    10681 ctgaattgct caagaacatc aagaaattgt cttatgtaaa cttgattcct tataacccag
    10741 ttagtgagca tgaccaatat agccgtagtc ccaaagagcg cgtgttggct ttctatgata
    10801 cgcttaagaa aaaagggggc aactgtgtgg ttcgtcaaga gcatggtaca gatattgacg
    10861 cagcttgtgg acaattgcgc tttaacacaa tgaaacgtga ccgccagaag gcagtcgcag
    10921 cggtaaatcc ttaatgatga agaagactta taatcatatt ttggtctggg gagtcatttt
    10981 ctatagcatt tgcattgtct atttttgctt tactcctcaa gaacaatcta ccgtgggagt
    11041 gggaactcca gatattcagc ctcttggacg cctggttttt cttttgactc ctttcaattc
    11101 tctctggaaa ctgggcgaag tgagtgacat tggacaatta tgttggattt ttttacaaaa
    11161 tatcctcaat gtcttcttgt tttttcctct gattttccaa ctcctttatc tatttccaaa
    11221 tttgcggaaa acaaaaaagg tccttctttt tagttttctt gtgagtcttg gaatcgagtg
    11281 tacgcaatta atcttggact ttttctttga ttttaatcgc gtctttgaga ttgatgattt
    11341 gtggaccaac actttgggtg gctatctggc ttggctcctt tataaacgat tacataaaaa
    11401 caagataagg aattaaaatg agtattttag aagttaaaaa tctgagtcac ggttttggtg
    11461 accgtgcaat ttttgaagat gtgtccttcc gtctcctcaa gggagaacat atcggcctgg
    11521 tcggtgccaa tggtgaagga aaatcaacct ttatgagtat cgtgactggt aaaatgctgc
    11581 cagatgaagg aaaggttgag tggtccaaat atgtgacggc tggttacttg gatcagcact
    11641 ctgtccttgc tgaaagacag tcggtgcgtg atgttctccg tacggctttt gatgagcttt
    11701 tcaaagctga agctcgtatc aatgacctct atatggaaat ggctgaagac ggtgcggatg
    11761 ttgatgctct catggaagaa gtgggagaac ttcaagaccg tctggagagt cgtgatttct
    11821 ataccttgga tgctaagatt gacgaagtag cgcgtgctct tggtattatg gactttggca
    11881 tggatacgga tgtaacttct ttgtcaggtg ggcaaagaac caaggtgctt ttggcaaaac
    11941 ttctccttga aaagcctgat attttgctgt tggacgagcc gaccaactac ttggatgctg
    12001 agcatattga ttggctcaag cgctatctcc aaaactatga gaatgccttt gtcctcattt
    12061 cgcacgatat tccattcctc aatgacgtta ttaatattgt ctatcatgtg gaaaatcaac
    12121 agctgacgcg ttactctggt gactactacc agttccaaga agtttatgct atgaagaaat
    12181 ctcagctaga ggcagcctac gaacgccagc agaaagagat tgcagacctc aaggactttg
    12241 tggctcgtaa taaagcccgt gttgcaactc gtaatatggc tatgtctcgt caaaagaaat
    12301 tggataagat ggatattatc gaactccaaa gtgagaaacc aaaaccatcc tttgatttca
    12361 aaccagctcg tacaccaggg cgctttatct tccaagccaa gaacttgcaa attggttacg
    12421 accgtcctct tactaagcct ttaaatctta ccttcgaacg caatcaaaag gttgcgatta
    12481 ttggtgctaa tggtattgga aaaacaactc tcttgaagag tctcttaggc attatctcgc
    12541 caatcgctgg ggaagtggag cgtggagatt atttagaact tggttatttt gagcaggaag
    12601 tagaaggcgg taatcgccaa actcctcttg aagctgtctg gaatgccttt cctgccctta
    12661 atcaagcaga agtccgtgca gcccttgccc gttgtggttt gacaaccaaa catattgaaa
    12721 gccagattca agtattatca gggggagagc aagccaaggt tcgtttctgt ctcttgatga
    12781 atcgtgaaaa caacgtttta gtgctggacg agccgaccaa ccatttggat gtggatgcaa
    12841 aggatgagct caaacgcgct ctcaaagaat ataggggatc tatccttatg gtctgccacg
    12901 agccagactt ttatgaaggc tggatagacc aaatatggga ttttaataat ttaacttaag
    12961 aatgagagta aaagaaatac agtaccgaat gtggatactg tatttttttg gtttttaaga
    13021 tttaaaatcg tagtctttca ccacttcgat gctgtcgatt gtgatagcag tagttggctt
    13081 gtctttttca tctttttcgg ccttagcaat cttatccaca acatccatac cgtcaatcac
    13141 ttgaccaaag actgggtgtt tgccatctag actagggttt ccaccttctt tgtaggcttc
    13201 gataattttc tgtggatact tgcttgtagg gagtttagaa gaggtatctg tagagttttg
    13261 gttgatgaag aactggctgc cattggtgtt tggttgacca gtattagcca tagcaagagc
    13321 accacggatg ttatacaaat aaggagtaat ctcgttcttg aaaccagttc ctttgtcttt
    13381 agtcttatcc ttgtcatgcc agatggactg accacctgta ccgtcccctt ttggatctcc
    13441 agtttggacc ataaagccat cgatgacacg gtggaaggta ataccgttat agtagccttc
    13501 tttggcgtga gtgaggaaat tttcaaccgc tagaggagcg agtttaggga agagtttaat
    13561 gcgaatatca ccttggcttg tgtggagaat cacttcggct tcatcttcag caacttcctt
    13621 agatagttgt gggaaattgg cgttttcgtt tgttaaagca tcgtttaact ccttggcaga
    13681 ttgggcagct actttggaac tttcttcagc agcaagactg gaatcaacat aatcatcacc
    13741 acgcagactg cgttggacgc tgctacaccc agctagggct acagtagaca gtaaaagaag
    13801 ggttgctagt tttttcattc tgatcctctc aaaaattcat ttataaccat tgtaccctaa
    13861 aaggaagtgg atttcaaata ttagtagttt acagaactgg cttagataat agttgggatt
    13921 tcatcatcat cttgaaaatc ttcaacttct tccccagtaa atggtttctg aaaagaactt
    13981 agtataaaac gggcaattgg ttcaacaatt aggaaattta gaggaagagc tgcaataaag
    14041 tttacgaacc aggcgcttag ataggtattg ggtgtgatag caatgccatt gtaaaaaaag
    14101 tccatagaag gacatgaaag tgaccagcta attcttcttc tgaaaaatgc tcatgaaggg
    14161 cttgatccag gtcgcaagat ttcattttag aaagtccagt aactcccttc ttcttcagga
    14221 tattcttttt catggtagta tttagatggt caagtgagtc aaaagaagtt tcgacaagag
    14281 catacccttt ttctaccaga gtttttagat gtttaggggc atcaatacca taagtgtact
    14341 caaagtattt tggaaaccag atcttaagaa agctcgtaaa gctagccagt tctctaaacg
    14401 ctaagaacta gctacaacac agcatttaca acacttcatg gttcacacca tggggtgttt
    14461 tttaatgatt ttaagcaaaa ttcacactta attgaaagtt atttaaatta gatgaaaatt
    14521 tattttttga aaaagtggga aaacattgat tctaagcgaa ttttgaatca tattcgaatg
    14581 ggagttttta aaatcatgtt tcagtggtaa aagtaccaaa ctggattctc catagtaaaa
    14641 tgatatctcc tgctagtgaa tcatctacta ataagtccat atttcgcttg ggaacgggct
    14701 tgggacttag aagccaagta tccaactctc gttcaggcga tatgaagggt ttgacttggt
    14761 gttctttata aaatttttct aaaatagtat tcacggatag catctttcaa acatttattt
    14821 cctattctaa caaatttcta aatactttaa gaaatttata gtctgtcaag tttttttctt
    14881 gacacatgtc agaaatctgc tataatagaa catgtgctaa atagctcagc tatttcaccg
    14941 aaataaaaaa ttaaagaaat gagactttaa aaatggcagt taaaatccgt ttgactcgta
    15001 tgggttctaa gaaaaaacct ttctaccgta tcaacgtagc agattcacgt tcaccacgtg
    15061 acggacgttt catcgaaaca gttggaactt acaacccact tgttgctgaa aaccaagtaa
    15121 ctttgaaaga agaccgcgtt cttgcatggt tggctaatgg agctcaacct tcagacacag
    15181 tacgtaacat cctttcaaaa gaaggcgtat tgaaaaaatt ccacgattct aaattttcaa
    15241 aataagttta aagtaggttg acagatggat acgattgaaa atctcattat tgcgattgtg
    15301 aaacccttaa tttcacaacc agatgcctta actatcaaga ttgaggatac accagaattt
    15361 ttggaatatc atttgaatct tgatcaaagc gatgtgggtc gtgtaatcgg tcgtaagggt
    15421 cgcactattt ctgcgataag aacgattgtc tactctgtcc caactgaata caaaaaagta
    15481 agaatcgtta ttgacgaaaa ataagaaggg cgggacggat gtctcgcttt tttgtgtgga
    15541 atagaagata tagtaaaatg aaacaagaac aggacaaatc gatcaggaca gtcaaatcga
    15601 tttctaacaa tgttttagaa gtaaaggtgt actattctag tttcaatata ctataaaaga
    15661 ttgaacgttt aggcaattcc aagactactt aatcgaacat taacagcagt ctcggactga
    15721 tgaaggtctc tttatgaagt tagtggagga agtcggaaaa gtagccgagg ttttgaatag
    15781 gcgctctggt cgaaaagaag ggataggatt cgaacgagga acttgccaga gaactggctg
    15841 atatcattta ctacaccgtc gcaatcgtag ctattaatca tattgacctt accaagactg
    15901 tctttgagaa agacaaaacc gcagccgtta attaccaaca taaacataat ttggaaggat
    15961 tattgaaggg aaagtagaga tgttgtcccc ttcctccaac ttgagaaaat ccttggaacg
    16021 tgataaaata aaggataatg atttttaata attcattata tagaatgagt ggatttcttt
    16081 atgaagaggt ttgaagtatc tacagaaatt ggtagcctct ctgttactta tcaaaagcaa
    16141 aagaaagtgc tagtttgtct aaacggagca ggtttgctac caagttatga aaatttttca
    16201 cttatacttg aaaaacctcc tcctacaatt ggttatttga caattgattt tccgaacaca
    16261 ggtaggagtc cgattcatga ccaagctgga aaaaatctag ataatcttgc agatgcggtt
    16321 tatgaagtac ttgaagaatt aggaatttct gaatatatac tttgtgcaca tagttggagt
    16381 ggaattttag cttgcaaatt actcgagaaa ccaattaagc gtcagacttt agtagcaatt
    16441 gaaccgacaa ctaaaaaagt catgtttgct gatttttcag aaaatcctta tccaaaaatg
    16501 gaagagcaga tgaggctgat tgacgagtgt ggtcctgaac tttattttaa gaacttaact
    16561 caagcaacat ttagtcctga aacgaataaa aaaatctggg aattaatgca agaaaaaggc
    16621 ttagagttgg aaaatcaaga tccagaattt cagatatctg gagagattac tgaggaagat
    16681 tttgagaatt tgtcgataga atctcatgtc cctgttttta ttttttgtca gacttataga
    16741 gaaaaagagt acagagaatc agaatattgg acttccaata ctaaactcat tttaggaggg
    16801 aatcaccatt atttacaatg gtcagaatcg gaaaaaattg cggctattat tcgagaattg
    16861 tcagaataag atggaaaaaa ggagattaca ggagacaaga tgaactactt taatattggg
    16921 aaaatcgtta atacgcaggg attacagggt gagatgcgag tcttgtctgt gacggatttt
    16981 gcagaagaac ggtttaaaaa aggagctgag ctggctttgt ttgatgaaaa agatcagttt
    17041 gtccaaacag tgaccatcgc tagccaccgt aaacagaaga actttgacat tattaaattc
    17101 aaagatatgt accatatcaa tactatcgaa aagtacaagg gatacagtct caaggtcgct
    17161 gaggaagatt tgaatgacct agacgatggt gaattttact atcacgagat tatcggtttg
    17221 gaagtctatg agggtgatag cttggttgga accatcaagg aaatcctgca accaggtgct
    17281 aatgatgtct gggtggtcaa acgaaaaggc aaacgtgatt tgcttttacc ttatatccca
    17341 ccagtggttc tcaatgttga tattccaaat aaacgggtcg atgtggaaat cttagaaggg
    17401 ttagacgatg aagattgata ttttaaccct ctttccagag atgttttctc cactggagca
    17461 ctcaatcgtt ggaaaggctc gagaaaaagg gctcttggat atccagtatc ataattttcg
    17521 agaaaatgct gaaaaggccc gtcatgtaga tgatgatccc tacggaggcg gtcagggcat
    17581 gttgctcaga gcacaaccta ttttcgattc ctttgatgct attgaaaaga aaaatccgcg
    17641 cgttattctc cttgatcctg ctggaaagca gtttgatcag gcttatgctg aagatttggc
    17701 tcaagaggaa gagctaatct ttatctgtgg gcactatgag ggttatgatg agcgcattaa
    17761 gaccttggta acagatgaga tttccctagg cgactatgtc ctcactggtg gagaattggc
    17821 agctatgacc atgattgatg ctacagttcg cctgattcca gaagtgattg gcaaggagtc
    17881 tagccaccaa gatgatagtt tttcttcagg tcttttagaa tatcctcagt acacacgtcc
    17941 ctatgattat cgaggcatgg tcgtgccaga tgtattgatg agtggccacc atgaaaagat
    18001 tcgtcagtgg cgattgtacg agagtttaaa gaaaacctac gagcgcagac cggatttact
    18061 tgaacattat caactgacag tagaagaaga aaaaatgctg gcagaaatca aagaaaacaa
    18121 agaataaagg agaaacctat gcaagtaatc aaacgtaatg gcgaaattgc tgaatttaat
    18181 ccagataaga tttaccaagc catcttgaag gcagcccaaa ctgtttatgt tttgacagat
    18241 gatttgcgtc aaaatcttgc tcaagtcact aagaaggtag ttttggattt acaagaagcc
    18301 aaggtggaac gtgcgactat cagtatgatt caatctatgg ttgaacatcg tttattgggc
    18361 gcaggttaca ttaccattgc agaacactac atttcctatc gtttacaacg tgacttggaa
    18421 agaagtggtt atggagatca tatcgcagtt catttacatt ttgaacaaat tcgctaagaa
    18481 aaaagagtgg gatgaagcaa ctacatctca ctctttctta aatctatttt tttgggctgt
    18541 ttggacagtt gaggggacaa atcgcaggcg ttgaatatcg cttatacgga taatccggct
    18601 cacatttttg gcaaaatttt tcacgataat ttgctggcct tgctgatcat atttgatgat
    18661 atcacctgta aagcttgtct cagacaagat aaggtgaaca gcggtttgtt tctggatagc
    18721 ctcttcaata gtggctgtaa gggagttggt ttcagtctgg tcaggttggt catgtccatt
    18781 taaaaaatca tgtattttat ctagaacttg ctggaattta tgtctcatat tggcctccct
    18841 tcttctatat caatattata taaaattttc ctaaaatcta caagatttta cgaataaact
    18901 aatgaaacta aaaaaggaga agaaaatggc agaatttaca tttgaaatcg aagagcactt
    18961 gttgactctt tctgaaaacg aaaaaggttg gaccaaggaa attaaccgtg tgagctttaa
    19021 tggtgcccct gcaaagtttg atattcgtgc ttggagtcca gaccatacta aaatgggcaa
    19081 agggattact ctctcaaatg aagaatttca aacgatggtg gatgccttta aaggcaacta
    19141 atactcttcg aaaatctctt caaaccgcat cagctttgcc ttgccgtatg tatggttact
    19201 gacttcgtca gtttcatcca caacctcaaa acagtgtttt gagcagcctg cggctagctt
    19261 cctagtttgc tctttgattt tcattgagta taagttttca aaataaaaaa aaaggatatt
    19321 gagtcatgat aactcgatat ccttttttag ttagtaaagt aagcctgatt tttaaggcgt
    19381 tgaagtaggg gacggctaat aaaactaata gccagaagtt tgccaaggtc tggaatgata
    19441 aagggaagaa cccccacaac aagagctttt tcaaatgcca ttccagctag gaaatgcaag
    19501 ctgagaatcc cgccgacaaa gacaagggca tcacccaaga ggtttgcaag aaaaatctta
    19561 acaacaccac tcttgctgtt ggttagagag gaagtaagtc cagagtaaac gagataaaac
    19621 caaagatagc ctgcagtagg gccaactaaa gcctgaaaac cagctccacc tcctgcaaag
    19681 acaggaagac cgatagcacc tagaagaaga tagagtccag cagaaagtac agcctctctc
    19741 ggtctaaaga cagtagcaat caagccgatt gcaaagtttt gcagagtgaa gggaacaggt
    19801 ccaattggaa gactgatttg tgccaaaaca gcaatgagag cagccccaat agcagggata
    19861 gcataaacgt gagctttttt caaaatagtt aacctctttt ctaatgtata gtaactatta
    19921 tactcatata ggagtcgatg tcaaccattt tttaaaaaca aggtaacaaa aactgtaaca
    19981 gccgattttc gtgactagtc aagttggata atttttttgt tgatt
//
LOCUS       DAVKFH010000029        18966 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKFH010000029 DAVKFH010000000
VERSION     DAVKFH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.029"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(318..2576)
                     /locus_tag="VW006_001245"
     CDS             complement(318..2576)
                     /locus_tag="VW006_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEU9380100.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2602..4119)
                     /gene="malQ"
                     /locus_tag="VW006_001246"
     CDS             complement(2602..4119)
                     /gene="malQ"
                     /locus_tag="VW006_001246"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9380101.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4669..5940
                     /locus_tag="VW006_001247"
     CDS             4669..5940
                     /locus_tag="VW006_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9380102.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6051..7343
                     /locus_tag="VW006_001248"
     CDS             6051..7343
                     /locus_tag="VW006_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9380103.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7345..8187
                     /locus_tag="VW006_001249"
     CDS             7345..8187
                     /locus_tag="VW006_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9380104.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8440..9240
                     /locus_tag="VW006_001250"
     CDS             8440..9240
                     /locus_tag="VW006_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9380105.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            9250..10236
                     /locus_tag="VW006_001251"
     CDS             9250..10236
                     /locus_tag="VW006_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9380106.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            10372..10452
                     /locus_tag="VW006_001252"
                     /pseudo
     CDS             10372..10452
                     /locus_tag="VW006_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10617..11558)
                     /locus_tag="VW006_001253"
     CDS             complement(10617..11558)
                     /locus_tag="VW006_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9380107.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(11536..13299)
                     /gene="aspS"
                     /locus_tag="VW006_001254"
     CDS             complement(11536..13299)
                     /gene="aspS"
                     /locus_tag="VW006_001254"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9380108.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(13580..13660)
                     /locus_tag="VW006_001255"
                     /pseudo
     CDS             complement(13580..13660)
                     /locus_tag="VW006_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(13696..13926)
                     /locus_tag="VW006_001256"
     CDS             complement(13696..13926)
                     /locus_tag="VW006_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380109.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(13944..14585)
                     /locus_tag="VW006_001257"
     CDS             complement(13944..14585)
                     /locus_tag="VW006_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9380110.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVAFAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(14597..15319)
                     /locus_tag="VW006_001258"
     CDS             complement(14597..15319)
                     /locus_tag="VW006_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9380111.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            complement(15322..15519)
                     /locus_tag="VW006_001259"
     CDS             complement(15322..15519)
                     /locus_tag="VW006_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380112.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNLYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(15519..15725)
                     /locus_tag="VW006_001260"
     CDS             complement(15519..15725)
                     /locus_tag="VW006_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380113.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(15735..16049)
                     /locus_tag="VW006_001261"
     CDS             complement(15735..16049)
                     /locus_tag="VW006_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380114.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     FQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(16304..17593)
                     /locus_tag="VW006_001262"
     CDS             complement(16304..17593)
                     /locus_tag="VW006_001262"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9380115.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETISMAAYFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(17671..18918)
                     /locus_tag="VW006_001263"
     CDS             complement(17671..18918)
                     /locus_tag="VW006_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9380116.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
BASE COUNT         5703 a         4123 c         3600 g         5540 t
ORIGIN      
        1 tgtaagcgaa tacaagagat atgaggtaac caactacgaa aaaatataag gctgaagaca
       61 gtaaaaacca gcatttaaag ccttcactgt atctaggatt tccctatttt tagacaagat
      121 tgacccaaat ccacagcatt taaaaaagcg aatttcaatc gaaactcgct tttttacaaa
      181 atgtattagt ataaatttgt atcttatact caatgaaaat caaagagcaa actaggaagc
      241 tagccgcagg ttgctcaaag caccgctttg aggttgcaga taaagctgac gtggtttgaa
      301 gagattttcg aagagtatta gttcaagtgc cagatgtctt cattatactg agcgattgta
      361 cggtcagatg agaagaatcc tgctttagaa atgttaacga tgactttatc caaccatgcg
      421 tcacggtctt cgtagtcagc aagcatttgc tctttgactt tgatgtagtc ttccaagtca
      481 agaagagtca tgaaccagtc tttgttgatc aattcattgt aaagacgttc caagcgctct
      541 ttgtttccag ctgcaagaac tgcatcacta acgatgaagt caaccaatgg tttgatagct
      601 tcacgagcgt agaattcgct tgatttgtaa gctgcttttg cgtaaaggtc gataacagtt
      661 tctgaatctt caccgaagat gtagatgttt tcttctccaa ccaactcagc gatttccacg
      721 ttagcaccgt ccatagtacc aagtgtcaaa gctccgttca acatgaattt catgttacca
      781 gtacctgaag cttctttaga agcaagtgaa atttgttctg agatatcaca tgctgggata
      841 aggaaacttg ctgcagtaac gttgtagttt tcaaccataa ctacttgcaa gtgtggagct
      901 actgctggat cgttagcaat aacttctgac atgcaaagga ttaaatggat aatgtcttga
      961 gcgattgtgt aggctggagc tgctttacca ccaaagaaga ttgtgattgg acgagcaggg
     1021 atgttaccag ctttgatgtc aaggtatttg tggatcacgt acaaagcgtt catttgttgg
     1081 cgtttgtact cgtgaagacg tttgatttgg atatcaaaga tagaatttgg attgatttcc
     1141 acaccttggt gttctttcaa gtgacgagcc aatttacgtt tgttgtgagc cttgatgctt
     1201 tccaattttt ctttgacagc tgctttgtct tcataagaca acagtttttc aagctcatct
     1261 gcttcatggt gccaaccatc tccaagaatc tcatccaagt agtgagacaa tcttgggtta
     1321 gcatgcataa gccaacgacg gaaagtgata ccgtttgttt tgttgttgaa cttttctggg
     1381 taaaggtcgt agaaggcttt caactcagaa tttttcaaga tttcagtatg gagtgctgca
     1441 accccgttaa cactgtatcc gtagtggata tccatgtgag ccatgtgaac acgtccgctc
     1501 tcatcgatga tttgaacagc tggatctttg tactctgcct tcacacgacg gtccaattct
     1561 tcgatgattg gtaccaagtg aggaaccact tcttgcaaga attcaagagg ccatttttca
     1621 agcgcttcag caaggattgt gtggttagtg taggcagtca tgctacgaac aattgagatt
     1681 gcttcgtcaa gatcgatacc acgtgcagtc aaaagacgaa tcaattcagg aatcaccatt
     1741 gatgggtgag tatcgttgat ttggacaact gcgtagtcag caaggtcatg caagttgctt
     1801 cctttttcga ttgcttcgtc gatgatcaat tgcgcaccgt ttgaaaccat gaagtattgt
     1861 tggaagatac ggagcaattc accttgacgg tcactatcat ctgggtaaag gaagagagtt
     1921 aagttgcgag cgatatctgt cttgtcaaag ttgataccat ctttaataat agaagaatca
     1981 actgaatcca agtcaaacaa acgcaagcgg tttttagtcg ctgtttcata accagtaaca
     2041 tcaatatcgt aaagagttga tgtcaaagta aagtctgcaa atggtacttg gtagctacgg
     2101 cttgagcgaa ccaaccagtt ttgctctgtc aaccatgcat ttggaattgt ttcttgttgg
     2161 ttgtttttaa gaacttgttg gaaaagacca aagtggtagt taagaccaac accgtcacca
     2221 ttcaaaccaa gagtagcaat tgagtcgata aagcaggcag ccaaacgtcc caaaccacca
     2281 ttaccaagag atggttccaa ttcaacttct tcaacttcga tcaagtcttt acctgcagct
     2341 gcaagttctt ttttaacatc gtcgtaaaga ccaaggttaa tcaagttgtt tgacaagagt
     2401 ttaccaatca agaactcagc tgagatgtag taaacttttt tcttaccagt gttgactggt
     2461 ttttggctgc ttgcaagctt gctgtagtta agaagagcaa ggtaaagctc ttcattgcta
     2521 cattctgcaa tggttttatt gtaacgattt tgtacaaatt cttgtagtga taacatgttt
     2581 agttgtctcc tgattattgt cttatttctt taaatctacc aaattttcat taattcggcg
     2641 ataaattgtt gtcaagtcaa gcaaaccttc ctcgacagct ggtgtcaatt gatcttcagt
     2701 catacgccaa gaccagtttc caccaagggt agatgggaag ttcatacgag ctgcctcatc
     2761 caattctagt aaatcttgca tagttgcaat tgccataaag ctaactgatg aaaatactgt
     2821 acgaagcata gcgtgtacca ctgtttcgta ttctttacgg ttcgtgtaac gagccatgta
     2881 ctcacgagtc gcatcatcaa tctcattacg gtaccaacca agaaccgtat tgttatcgtg
     2941 tgttcctgtg tacataactg agttagcagg tgccaagtgt gggctatcaa tgctttcgtc
     3001 ttctgggttg aaggcaaatt gaagaatctt cattcctggg aagccagtac gttcacgcaa
     3061 ttcgatcact tcatctgtca tgaagccaag gtcttctgcg atgatgttta gctcaccaag
     3121 ttcttcctta acggctgcaa aaagcttgta gcctggacct ttcacccact caccaggtgc
     3181 tgctgtatcg gaaccagcag ggatttccca gtaagattcg aagccacgga agtggtcgat
     3241 acgaacgata tcgtagattt tgaagctttc acgcaagcgt tcaatccacc atttgtagcc
     3301 gtctttgtcc attgcttccc agtcatagat tgggttaccc caaagctgac cagttacaga
     3361 aaactcatct ggtgggcatc ctgcgataca agtagcctta ccattgacat ctgttttgaa
     3421 gagatgtgga tttgcccaca tatcacttga atcttccgct acgtagattg gcatgtcccc
     3481 aacgatttcg atgtggttgt cgttagcgta agctttcaat ttcaaccatt gttggaagaa
     3541 gaagtattga gtcacacggt ggtaaaccaa cttgtctgcc aattgctcac gatagctttc
     3601 aagtgctgaa gctttacgag cacgagcatc tgcatctggc cattcagtcc aagcaagatt
     3661 gtcaaaatac tctttgatag ccatatactc agcaaagagc tcaagccatg attggttgtc
     3721 ttgagcaaat ttctcaaaat ctttaacatc tccgacttca aagaaacgtt tcaccgcttt
     3781 ttctaaaaga ggacgacgtg catagtagat tttagcatag tcaacttcag acgcatcgct
     3841 accaaagtca actccttcaa ggtcacttgc ttccaacaaa ccttgctcca ccaagatatc
     3901 taaatcgata aaatgagtgt ttcctgcgaa ggctgagaaa gattggtaag gagaatcccc
     3961 gtaactagtt gctcctaatg gaaggatttg ccagtaacgt tgttttgtac ggaccaagaa
     4021 atcaacgaag tcgtaagcac tttgaccaaa tgatccgatt ccgtaagctc ctggaagaga
     4081 agagatgtgc atcaacacac cactttgacg ttttttcata atagcacctc gtgtgttaaa
     4141 ataatggaac gttgcgtatt ttgcagacgc aaacgtttgc gttacttata agtatactcc
     4201 ttttcaagca taattgcaag cgtttttaaa aaactttttt gattattttt aaggagacta
     4261 actcatttta gcaaagataa cttaaaacta tctaatccta aggtttgact ctcacgagac
     4321 agtctacaaa gtacaaaacc tccttaggga gcttgaattt atatgtaaca aagcacaaac
     4381 gtccatagaa aatagatagg gattagaacc agggaggtag cccctcctgg tttccctctt
     4441 tagacagatt ccatatattt ttattaaaaa tactgtattc ttatctattt catacttttc
     4501 ttctattaaa atgcaaaatt atgctatcaa atcaagctta aaaatttttt aaaattttta
     4561 caaaaaatac ttgcaaccgt tttctatttg tgctatacta agctcataaa ggaaaacgtt
     4621 tgcgtttcct tatcaataaa acttgctatt ctttgggagg aatacactat gtcatctaaa
     4681 tttatgaaga gcactgcggt gcttggaact gttacacttg ctagcttgct tttggtagct
     4741 tgcggaagca aaactgctga taagcctgct gattctggtt catctgaagt caaagaactc
     4801 actgtatatg tagacgaggg atataagagc tatattgaag aggttgctaa agcttatgaa
     4861 aaagaagctg gagtaaaagt cactcttaaa actggtgatg ctctaggagg tcttgataaa
     4921 ctttctcttg acaaccaatc tggtaatgtc cctgatgtta tgatggctcc atacgaccgt
     4981 gtaggtagcc ttggttctga cggacaactt tcagaagtga aattgagcga tggtgctaaa
     5041 acagacgaca caactaaatc tcttgtaaca gctgctaatg gtaaagttta cggtgctcct
     5101 gccgttatcg agtcacttgt tatgtactac aacaaagact tggtgaaaga tgctccaaaa
     5161 acatttgctg acttggaaaa ccttgctaaa gatagcaaat acgcattcgc tggtgaagat
     5221 ggtaaaacta ctgccttcct agctgactgg acaaacttct actatacata tggacttctt
     5281 gccggtaacg gtgcttacgt ctttggccaa aacggtaaag acgctaaaga catcggtctt
     5341 gcaaacgacg gttctatcgt aggtatcaac tacgctaaat cttggtacga aaaatggcct
     5401 aaaggtatgc aagatacaga aggtgctgga aacttaatcc aaactcaatt ccaagaaggt
     5461 aaaacagctg ctatcatcga cggaccttgg aaagctcaag cctttaaaga tgctaaagta
     5521 aactacggag ttgcaactat cccaactctt ccaaatggaa aagaatatgc tgcattcggt
     5581 ggtggtaaag cttgggtcat tcctcaagcc gttaagaacc ttgaagcttc tcaaaaattt
     5641 gtagacttcc ttgttgcaac tgaacaacaa aaagtattat atgataagac taacgaaatc
     5701 ccagctaata ctgaggctcg ttcatacgct gaaggtaaaa acgatgagtt gacaacagct
     5761 gttatcaaac agttcaagaa cactcaacca ctgccaaaca tctctcaaat gtctgcagtt
     5821 tgggatccag cgaaaaatat gctctttgat gctgtaagtg gtcaaaaaga tgctaaaaca
     5881 gctgctaacg atgctgtaac attgatcaaa gaaacaatca aacaaaaatt tggtgaataa
     5941 aaaatttgtt caaggggggt ggaaatcaaa tccccctttg aatttatcaa tagagacaca
     6001 aataatttag ctttcttata aaaaagtagt atcctatgaa aggagttaat atggaaaagc
     6061 aacaacctag taaagcagcc ctgctgtcta tcattcctgg gttaggacag atttacaata
     6121 aacaaaaagc caaaggtttt atcttccttg gtgtaaccat cgtatttgtc ctttacttcc
     6181 tagcacttgc aacccctgaa ttgagcaacc tcatcactct tggtgacaaa ccaggtcgtg
     6241 ataattccct ctttatgctg attcgtggtg ccttccatct aatctttgta atcgtttatg
     6301 tactctttta tttctcaaat atcaaagatg cacatacgat tgcaaaacgc attaacaatg
     6361 gaattccagt tccacgcaca ctcaaagaca tgatcaaagg gatttatgaa aatggcttcc
     6421 cttacctctt gatcattcca tcttatgttg ccatgacctt cgcgattatc ttcccagtta
     6481 tcgtaacctt gatgatcgcc tttaccaact acgacttcca acacttgcca ccaaacaagt
     6541 tgttggactg ggttggtttg accaacttta caaacatttg gagcttgagt accttccgtt
     6601 ctgcctttgg ttctgttctt tcttggacta tcatttgggc tttggcagct tctactttac
     6661 aaatcgtaat tggtatcttc acagctatca ttgccaacca accatttatc aaaggaaaac
     6721 gtatctttgg tgttattttc cttcttcctt gggctgtccc agccttcatc actatcttga
     6781 cattctcaaa catgtttaac gatagtgtcg gtgctatcaa cactcaagta ttgccaatct
     6841 tggctaaatt ccttcctttc cttgatggag ctcttattcc ttggaaaaca gacccaactt
     6901 ggactaagat tgccttgatt atgatgcaag gttggctcgg attcccatac atctacgttc
     6961 tgaccttggg tatcttgcaa tctattccta acgaccttta cgaagcagct tatattgacg
     7021 gtgccaacgc ttggcaaaaa ttccgcaaca tcactttccc aatgattttg gctgttgcgg
     7081 cacctacttt gattagccaa tacaccttca actttaacaa cttctctatc atgtacctct
     7141 tcaatggtgg aggacctggt agtgtcggag gtggagctgg ttcaaccgat atcttgatct
     7201 catggatcta ccgtttgaca acaggtacat ctcctcaata ctcaatggcg gcagctgtta
     7261 ccttgattat ctctatcatt gtcatctcaa tctctatgat cgcattcaag aaactacacg
     7321 catttgatat ggaggacgtc taagatgaat aactcaatta aactcaaacg tagactgact
     7381 caaagcctta cttaccttta cctgattggt ctatcaattg taattatcta tccactgttg
     7441 attaccatta tgtcagcctt taaagcaggt aacgtctcag cctttaaact agatactaat
     7501 atcgacctca attttgataa ctttaaaggc ctcttcactg aaaccttgta cggtacttgg
     7561 tacctcaaca ctttgattat cgccttaatt accatggctg ttcaaacaag tatcatcgta
     7621 cttgctggtt atgcttacag ccgttacaac ttcttggctc gtaaacaaag tttggtcttc
     7681 ttcttgatca tccaaatggt gccaactatg gccgctttga cagccttctt cgttatggcg
     7741 cttatgttga acgcccttaa ccacaactgg ttcctcatct tcctctacgt tggtggtggt
     7801 atcccgatga atgcttggct catgaaaggc tacttcgata cagtgccaat gtctttagac
     7861 gaatctgcaa aactagacgg tgcaggacac ttccgccgct tctggcaaat tgttcttcca
     7921 cttgttcgcc caatggttgc cgtacaagct ctctgggcct tcatgggacc tttcggggac
     7981 tacatcctct ctagtttctt gcttcgtgag aaagaatact ttactgttgc cgtaggtctc
     8041 caaaccttcg ttaacaatgc gaaaaacttg aagattgcct acttctcagc aggtgctatc
     8101 ctcatcgccc ttccaatctg tattctcttc ttcttcctac aaaagaactt tgtttcagga
     8161 cttacaagtg gtggcgacaa gggataattt atccccgcca ccctttttca ttttatactc
     8221 ttcgaaaatc tcttcaaacc acgtcagctt tatctccaac ctcaaagttg tgctttgagc
     8281 aacctgtggc tagtttgcac tttgattttc attgattatt agcaattgtc actgtaaata
     8341 atatccttgt agcaagcaat ttttctccta gacttgaaat aaagcgcatt tctctatata
     8401 ataatactta tatagaaaac accttttaga aagataccta tgcttccata tccattttcc
     8461 tttttttcaa gtattttggg gtttcgtaag cccctgtcca aacgtttcgg gctcaactgg
     8521 tttcaacttc tctttaccag tatcttcctt atcagcttgt ctatggtacc cattgctatc
     8581 caaaacagct cccaggagac ctatccgcta gaaactttta tcgataatgt ctatgaacct
     8641 ctgacaaatg aggttgtcca ggatctctct gaacatgcta caattgtcga tggcacatta
     8701 acttatactg gaacagttag tcaagcctct tctgttgtga ttggtccaag tcaaatcaag
     8761 gaattaccta agaacttgca actgcatttc gatacaaatg agctagtcat cagcaaggaa
     8821 agcaaggaac tgacccgcat ctcttaccga gccattcaga ctgagagttt caaaagcaaa
     8881 gacagcttga cccaagcaat ttctaaagac tggtaccaac aaaatcgtgt ctatatcagc
     8941 ctcttcctag ttctcggtgc gagcttcctc tttggtttga atttctttat cgtctctcta
     9001 ggagctagtc ttctccttta tatcactaag aaatcacgcc tcttttcatt tagaaccttt
     9061 aaagagtgct atcactttat cttgaactgt ttaggattgc cgactctgat tacacttatt
     9121 ttgggattat ttggccaaaa tatgacaacc ctgattactg tacaaaatat tctttttgtt
     9181 ctgtatctgg tcactatctt ttataaaaca catttccgtg atccaaatta ccataaatag
     9241 gagattttta tgcccgttac gattaaagac gtggccaagg ctgctggtgt ttcgccttca
     9301 accgtaaccc gtgttattca aaataaatca accattagcg acgaaacaaa aaaacgcgtt
     9361 cgcaaggcta tgaaggaact caactaccac ccaaacctca acgctcgtag cttggtaagc
     9421 agctatactc aggttatcgg attagttctt cctgatgact cagacgcctt ctaccagaat
     9481 cctttctttc catcggttct acgtggcatc tctcaagtcg catctgaaaa ccactatgcc
     9541 attcagatag caacagggaa agatgagaag gagcgtctca acgctatttc acaaatggtc
     9601 tacggcaagc gtgtagatgg gctaatcttt ctctatgccc aagaagaaga ccctctcgta
     9661 aaactcgtcg cagaagaaca gttccccttc cttatcctag gtaaatctct atctcctttc
     9721 atcccacttg tcgacaacga caatgttcaa gctggttttg atgcgactga atatttcatc
     9781 aaaaaaggct gcaaacgcat tgcctttatc ggaggaagta aaaagctctt cgtgaccaaa
     9841 gaccgtttaa caggctatga acaggcgctt aaacattaca aacttaccac tgacaacaat
     9901 cgcatctact ttgccgacga gtttctggaa gaaaagggct ataaatttag caagcgatta
     9961 ttcaagcacg atccacaaat tgatgctatc atcacaaccg atagcctcct agctgaaggt
    10021 gtttgtaact atattgccaa acaccagctg gatgtccctg ttctcagctt tgactcggtt
    10081 aatcccaagc tcaacttggc agcctatgtc gatatcaata gtttagagct tggtcgtgtt
    10141 tcccttgaaa ctattctcca gattattaat gataataaaa acaataaaca aatttgttac
    10201 cgtcaattga tcgcccacaa aattatcgaa aaataagaga ctgggcaaag agtcgttaaa
    10261 agcaaaaacg catactatca ggtattgaaa aaacttgata ctatgcgttt tattgtggga
    10321 agatttactt ccttttctac tgaaattgag tcttttccca agatcttttt atactcaatg
    10381 aaaatcaaag tgcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt
    10441 gtggatagaa ctgacgaagt cagctcaaaa catggttttg aggttgcaga tagaactgac
    10501 gaagtcagct caaaacatgg ttttgaggtt gcagatagaa ctgacgaagt cagtaacata
    10561 tatacggcaa ggtgaagctg acgtggtttg aagagatttt cgaagagtat taatcactaa
    10621 ttatctatct caacaaatct tcctagaata tgaacatttt ccgagacaga gacaaaggag
    10681 cttggatcca cttgtgtcat aatctgttta aattcattaa actctgcacg tgtaatgaca
    10741 gtgattaaaa ctgcctttct ctcgtgatta taggttcctt ctgcatcgtg gatcatggtt
    10801 gctccgcggt gcaatttttt atggattttt tcaattacct tctctggatg atttgtcaca
    10861 atcatggcct gcatacgctt ttgcttagta aagactgcgt ctgtcacacg gctagagaca
    10921 aagatggtaa tcatagaata aagagcgtat ttccaaccaa aggtcaaacc tgctatcagc
    10981 atgatagttc catttaccaa gaaagaaata ctaccgacat tcttacccgt tttcttgcga
    11041 atagtcaggc tgacgatatc cgtcccacca ctggagatat tgtttcgaag agcaaaacca
    11101 atccccaaac ccataacaac acccccaaaa agggaattga taatgggatc ctccgtcaag
    11161 gttgccacag ggacaaactg gataaagaag gaactcatag ataccgtgat aaaggtaaag
    11221 acggtgaact tatggccaat ctgataccaa gctaagacca tcaaagggaa gttaatggcg
    11281 tagaagctta gcgaaatcgg aatatgaaaa ccaaaccagt gattactcaa ggcagagata
    11341 atctgtgcca gacctgttgc accactcgaa tacacatgcc ctggttggaa aaagaaatta
    11401 actgctactg ctgataaaaa accatagacc agagaggccg aaatcttctc atcatacttt
    11461 tctcgagaga tactttgtaa gacacgtaaa atttttatct gataagcaaa gcggcgcaga
    11521 taatagcgcc accgcttaat tcgttttgtt tgtttcatct tcttctactt gtaagctgag
    11581 ttcctctagt tgtttgagag cgactgttga tggagcttgt gtcattgggt cagttgcctt
    11641 gttgttctta ggaaaggcaa tgacttcacg gatattttct tctccagcaa gcaacatgac
    11701 aaaacggtca agcccgatag ccaaaccacc gtgtggtggg aaaccatagt ccatggcttc
    11761 aagaaggaaa ccaaactggt cattggcttc ttcagttgag aaaccaagag ccttgaacat
    11821 gcgttcttga aggtcttttt ggttgatacg aaggctacca ccaccaagct cataaccgtt
    11881 caagacgata tcgtaagcaa tggcacgaac cttagccaaa tcaccttcta attcgtgagc
    11941 agtctcttcc tgtggaagtg tgaatggatg gtgggcgctc atgtagcggc cttcttcttc
    12001 agaccattca aacatcggcc agtcaaccac ccaaaggaag ttgaacttat cattatcaat
    12061 caagccaagc tctttagcaa tacgtccacg aagggcaccc agtgttgcat tagccacttc
    12121 aagcgtatcc gccacaaaga gaaccaagtc cttatcttca agagcaagcg ctgttgtcaa
    12181 ttcttcttgg ataccagtca agaacttggc aactggtccg tttaattctc catcaaccac
    12241 cttgacccaa gcaagacctt tggcaccata ctgtttggct acttccgtca tcttgtcgat
    12301 gtctttacgt gaatagttgt ccgcagctcc tttgaccaca atcgctttta cagcaggtgc
    12361 ttctgaaaag actttaaagt ctacaccttt gaccacttct gtcaagtcct gaagcaacat
    12421 gtcaaaacga gtatctggct tgtcagaacc gtaaagagcc atagcatcat cgtatttcat
    12481 acgagggaac ggtagcgtta cttcgatgcc ttttgtttcc ttcatcacgc gcgcgatcaa
    12541 gccttctgta atatcttgga tttcttgctc agtaaggaag gacgtttcca agtcgacctg
    12601 agtaaattca ggctggcggt ctccacgcaa gtcctcgtca cggaaacatt taacgatttg
    12661 gtagtaacgg tcaaaaccag cattcatcaa gagctgtttc gtgatttgtg gactttgagg
    12721 aagagcgtaa aaatgcccct tattaacacg agacggcact aaataatcac gcgccccttc
    12781 aggcgttgac ttagaaagga atggtgtctc cacgtcgata aactccaact catccaagta
    12841 gttgcggata gagtgggtca ccttggcacg aagtttaaga ttttccaaca tttctggacg
    12901 acgaaggtca aggtaacggt aacgcaaacg tgtatcgtca tttgcctcaa tgccatcctt
    12961 aatctcaaat ggtgttgtct tagctgtgtt aagcacaata agagctgtca cgtttaactc
    13021 aaccgcacca gttggcaact tatcattggc ttgctcacgc gcagcgacct gaccagtcac
    13081 ctcaataaca aattcgctac gaaggctttc agctgttgcc ataacctctg cagatacttt
    13141 ttcagggttg ataaccaact gcatgattcc ttcacggtca cgaagatcga taaagatcaa
    13201 accaccaagg tcacgacgac ggccaaccca tcctttcaag gttatttctt gtccgatgtg
    13261 ttcctcacga acacgaccag catacatact acgtttcatt atttctctcc tcttttattc
    13321 tgttactatt ttaccataaa agcgcaggct cttcatgaaa atcatcagaa aagtttgcca
    13381 gtctttaaaa gtcaggtgaa agccctaaaa attagcgcta atactcttcg aaaatctctt
    13441 caaaccacgt cagcgtcgcc ttaccgtagg tatggttact gacttcgtca gtttcatcta
    13501 caacctcaaa accatgtttt gagctgactt cgtcagtttc atccacaacc tcaaaaccat
    13561 gttttgagct gactttgtca gtttcatcca caacctcaaa acagtgtttt gagcaacctg
    13621 cggctagctt cctagtttgc tctttgattt tcattgagta taatacaaaa atccgatgaa
    13681 cttcaccgga ctcttttatt ttgaattttt gcctgcttta cgcttttcag cgatttcggc
    13741 tgcctttcga ggcaagacaa tttccgttat gtaagccgtc ccaaaacgca gtacacctgc
    13801 aataggagca aagacaactg ctagatagtt atagaagaaa tcgcctttga aggcataagc
    13861 tagcgctcca atgatgaaaa atagaacgac tgcctgaatc actgctaata aaattactcg
    13921 tttcatgtga cctcctgact ctattatagc atgagaatca tcaaaaagcc gactaaatta
    13981 ttcaaagcgt gaagagaaat actgtagacc agaccttttc tgctaatgta agccaaaccc
    14041 aaactaaaac caaggctaaa atagacaaaa aattgttgca catcacctgg aaaatgaatc
    14101 aaggcaaata gaagactaga taccagaaga aaaatcaggg ttcgtttact attgtcctgc
    14161 ttaggaaaga gatagcgtgc taacatccct ctaaaaacaa tctcttccgt caaaggagca
    14221 aaaataacca cagcaaagaa tgagaaaagt ggttgagaca aggtcaagtc tgtcgctatt
    14281 tgctgattta ctgaaggatc atctggcaag aagaattgaa cgaccagaga taagaaccaa
    14341 accaagacag gaagccaaat aaatcgatta aagccgctct tctcaatatg aacaggagcc
    14401 ttctgatacc atttgtaaat gccgtacaca tatactccag caaaggccac atagagtaga
    14461 gtaacagcat agggtgaagc gcctaaagca agcgacgcag tcgcgagccc ctgaataaag
    14521 ccatagataa ataaaaagga tagaagggct agaagaatcc agccaaggtt tttaagtaat
    14581 ttcatagata actcctttat ttgaaataac gttttaccat aggtaactgc atcacattga
    14641 tataaacatg gatggctcct acaagcaaga aagctagtaa ctgaatctct cctgtcaaga
    14701 aagaaatgat aataagaaaa atatataagg ctggtaagac atattggtgt aattggaata
    14761 aaattcgaaa actctgttcc aaattagcct gacgctcccc ttcatcataa gaatttatat
    14821 agttcaagac atcctttggt gtagcgaaaa attccaaatc aaactgacga acaatcgcaa
    14881 tggttttaaa aagagatttt tgagcgacta agaataccac aaagagtaag aaagaaagga
    14941 aaaatgtttg agggtttgta tgcaatataa tcacctcact taatgaaata aaaatagcca
    15001 atggaatcgc tacacctgta atattaaaag caatggttcc aaactcaaga ttccgataca
    15061 tttgcacata ataggtttca ttcagatcgt catccatttc ctcttgatac aaggaatgaa
    15121 attttctgct tttctttaag aaattgaaag tcaaaaacat actaatgaaa cctatcagta
    15181 aacaaatagc tgatatccat ggcatcaagg cttttacatc taaaataatt tcgtgggatt
    15241 cgacacgtgc cttaaacatc cctacaaaca tgcccaagac aatagacatc aaaaataaca
    15301 atctacgttt ctttttcata ttcattctcc tttttcactt gctagatttt tggatttctt
    15361 ttcaatccat tcaattactg ggatgagagc aaagtagacc caaacaaatt ggtcgctttg
    15421 atagagatta aaccagctta ggtccatccc aatcagtaga aatacactga ctaataaagc
    15481 tatgaccact acataataaa tcactttata cttgttcatc actcgtcctc ctccaaacga
    15541 aataccgatt cgactgtttc gttgaaaatt tgagatattt tcagggcaat gataatggat
    15601 ggggtgtact catcccgttc tagtaggcta atggtctgtc tggaaacccc tgccagtttg
    15661 gctaggtcgg tttgattgag accatcgcga gctctaagct cttttagacg atttttaagt
    15721 tgcatgttac acacctactc tccgtcaaat tcaacggttt ggatatcctc aatgcgttgc
    15781 aacttgaatt tttcttttcc cgtattatct acacgtcgta gctttaccca ttcctcatca
    15841 acatccacaa cttcccagtt atctggccca atatacactc ccgttataat tggttccttt
    15901 ccaatcattt cttgaaataa tctcgacatt tctgcgtttc ctttctcttt tcgctcaagt
    15961 tttttgattt tattctctag tttcttgatt tttttagaat tattagaata aaagaaaatc
    16021 ataaatagta taaatcctag tacccacatc atagctcctt tcttcttttc tcttaacttg
    16081 cttacatcgt aacacatctt tttctttttg acaagtatag ttgtcaaaaa gtttatgatt
    16141 tttgtcattt tgcaaaggaa aaaggtcagg agtaagttcc tgaccacttt atctatcatt
    16201 aatactcttc taaaatctct tcaaaccacg tcagcttcat ctacaacctc aaaaccatgt
    16261 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaaatc
    16321 ctagtttttc aaagatttct gagaagtttt ggctgattgt ctcaagtgac acttgcactt
    16381 cttctcgggt ttggttgttc ttgaccgtca cttgtccgct ttcgacttcg ctctctccta
    16441 gggtgatgag ggtcttagcc gcaaagacat ctgctgactt gaactgagct ttgagcttac
    16501 ggttgaggta atcacgctct gctttgaaac cttgttggcg aagagcttgg actagttcta
    16561 gggctttgac atttgctcca tcacccaaga ctgcgatata gacatctagg gcgttttcga
    16621 tagggagggc cacgccttgt ttttcaagga tgagaagcag gcgctctaca ccaagcccaa
    16681 aaccaaatcc agcagtttca gggcctccaa agtaagcaac caaaccatcg tagcgaccac
    16741 ccgcacagac tgtcaagtca ttgccctcaa tctctgtgat aaactcgaaa atggtgtggt
    16801 tgtagtagtc cagaccacgc accatattgg tatcgatgat gtaatctact ccaagatttt
    16861 ccaacatctg acgcacagca tcaaaatgag tttggctttc ttcatcaaga aagtccaaga
    16921 tagacggcgc attctctact gccaccttgt cttctttttc cttagagtcc aagacacgaa
    16981 gaggattttc ctccaagcga cgttggctat ccttagacaa ggtctccttg agcggtgtca
    17041 aatagtcaat caaggcttgg cggtaggctg cacggctctc aggatttcca agagtgttga
    17101 ggtgcaattt gacaccttga ataccgattt ccttcaaaaa ataggctgcc atagagattg
    17161 tttccacatc ggtagctgga ttgctagagc caaaacactc aacaccaatc tggtggaatt
    17221 ggcgcaagcg ccctgcctgt ggacgctcat aacggaacat aggtcccata tagtagaact
    17281 tgcttggctt ttgcacttct ggggcgaaga gtttattttc cacataggaa cggacaacgg
    17341 gcgcagttcc ttctggacgg agggtaatat gacggtcacc cttgtcataa aaatcgtaca
    17401 tttccttggt tacgatatcc gttgtatctc cgacagagcg actgataacc tcgtaatgct
    17461 caaaaatagg cgtgcgcact tctgcatagt tatagcgctt gaaaatctca cgggcaaagc
    17521 cctcaacgta ctgccactta gcagattcag caggtaaaat atcctgcgtt ccttttggtt
    17581 tttgtaattt catagggaat cctctttaaa cttaatagtc ttattttacc acaaataaag
    17641 ggattaaaac agtaagaaaa aaattaggat ttagatatca tttttgagat taagaattgt
    17701 caaaaaaata gctagcaagg aaagaccaac aaatagcatc caagtcaact gtatattcca
    17761 tacggctact agtgaaaaac aagctgttcc cacaggtatg gataaggtaa acaatagacc
    17821 taaaaaatta ctagtacgag ctagaacctc tggagctaga tttttcatga gcatggcact
    17881 aatctttggt tgaactttac cagacacata cagggtaaag aagagaaata gcaaaccaag
    17941 cacgacttga ttgaataaat tagccaaacc aactagacta agtcctacgg tcgcccacat
    18001 catcaatcta ggcaaggact gcttcccaaa ataatcattg cccgtaaggc tactgatgat
    18061 gattgctact aaaacacaga attgattgat aaatagtgcc tctgtataag aaaaattcaa
    18121 gagagaatgg ctcaaaaaga agatattata aattccaccc aaagcgccac ccaaggaatt
    18181 aataagcaag acagcaaaga gcataaaacc aaagtttttc tgcccacttt taagaaaaac
    18241 gagacgtaaa tttcggtaaa ttgttaggaa ctggtctttg atagaaagct tctcattttt
    18301 taagttttca ccatcagcag atgacattga caggctcaat ttgctttttc ctaaaaagag
    18361 gatagtggct gatactagga aaaagcaggc attgattccc gcaacgagag aaaaattgtt
    18421 gaccgataga gctaagagcc agactccgaa agcttgacca ccaatagctg aaatataggt
    18481 gatgaactgt gaaaaagaat aagcctccat cagatcatct tcagctactt tttccttaat
    18541 aagaggcatg cgcaagccac ctgcaaaatc actgatgaca tcactaatga cattgatcaa
    18601 acacaggcta gaaaaggcaa agagactagc ttgctgaaca actagggctg ctaggaaaaa
    18661 tagaaccgcc tgaaacaaac cgctatagac catccatttg accttgtccc tcgtgtaatc
    18721 tgcccgaatc cctacaaaaa ctgtaaagag ggtcggaaga atcatgacaa tattcgccat
    18781 agcaacagca aaagatgctt gtgacaaggt cgatgcatag acgataaaga ccaggttgaa
    18841 aatcgaagca ccaaaagcat tgaagaagcg tgataaagtc aaaattctat aagctggatt
    18901 tctaaacaat agtttcatag ataatctccc ttcttgttct aagttctaca tcaagaatag
    18961 ctttag
//
LOCUS       DAVKFH010000030        18744 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKFH010000030 DAVKFH010000000
VERSION     DAVKFH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.030"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            510..2663
                     /locus_tag="VW006_001264"
     CDS             510..2663
                     /locus_tag="VW006_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9380117.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKANMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2676..4025
                     /locus_tag="VW006_001265"
     CDS             2676..4025
                     /locus_tag="VW006_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9380118.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4195..4902
                     /locus_tag="VW006_001266"
     CDS             4195..4902
                     /locus_tag="VW006_001266"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9380119.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4959..8684
                     /locus_tag="VW006_001267"
     CDS             4959..8684
                     /locus_tag="VW006_001267"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9380120.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8777..10219
                     /locus_tag="VW006_001268"
     CDS             8777..10219
                     /locus_tag="VW006_001268"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9380121.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10256..11278
                     /locus_tag="VW006_001269"
     CDS             10256..11278
                     /locus_tag="VW006_001269"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9380122.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11275..11820
                     /gene="purN"
                     /locus_tag="VW006_001270"
     CDS             11275..11820
                     /gene="purN"
                     /locus_tag="VW006_001270"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9380123.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11904..12413
                     /locus_tag="VW006_001271"
     CDS             11904..12413
                     /locus_tag="VW006_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9380124.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12438..13985
                     /gene="purH"
                     /locus_tag="VW006_001272"
     CDS             12438..13985
                     /gene="purH"
                     /locus_tag="VW006_001272"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9380125.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQEFIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14107..15369
                     /gene="purD"
                     /locus_tag="VW006_001273"
     CDS             14107..15369
                     /gene="purD"
                     /locus_tag="VW006_001273"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9380126.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15772..16260
                     /gene="purE"
                     /locus_tag="VW006_001274"
     CDS             15772..16260
                     /gene="purE"
                     /locus_tag="VW006_001274"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9380127.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16247..17320
                     /gene="purK"
                     /locus_tag="VW006_001275"
     CDS             16247..17320
                     /gene="purK"
                     /locus_tag="VW006_001275"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9380128.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVGEF"
     gene            17343..>18744
                     /locus_tag="VW006_001276"
     CDS             17343..>18744
                     /locus_tag="VW006_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9380129.1"
                     /translation="MFEKIKGINIKSGIFEDETKLELFEGNFEGTNPVQNDRASLLFG
                     RNGSGKSTIARGINQLKNGEIGTDRVSFIDKNNNNIVLSDTERKSIFVFNEHYVDQKV
                     KIAQEGLDTIVILGEQVDIDEELDRLRTQLSESQIESQDYYAEYEEYLDEKNEKSPDF
                     WKKEMTDSLKGVGNWAERDREIKGNRAASPVHNNTFQNFVDLQPILDKNELEVEFNNK
                     KARYFSIRDSAVTINNELSLPDINFDSNELSTLLSEKIEEPELNSRDKYLLTLLSDST
                     KGERHLREVKDFFEDEHQKKCPFCTQSVSEDVKVELTNGITKLLSRAVEEHQSALRGK
                     KIDEINQDFSGYEQIDPILIQSYQNSINALNAKFNEINSIIDKKIDNPYNIVELPNIS
                     FSQELSQAEHDIEKINQAIIKHNSEISGIQKLKVDLLQINNELAFYEIQDAYKKFQEK
                     TNKKAICENNYNNSSKR"
BASE COUNT         5396 a         3631 c         4480 g         5237 t
ORIGIN      
        1 tttcagcgta ttgacagtat tactaattgg gataacaaat tatgcgagat ttaaaaattt
       61 aagattgaca gtagtgtttt ctgtaccatt tttatttaat gctataatgt ctattataaa
      121 tagtttttag aaatactaaa atttttttct ttacttaata atgatatgaa atcatttcgg
      181 ttttgttaac ttgtaattct tatattttct aggtagaacg tttgttctgc ctattttttt
      241 atccaaaaag tgcagttggg agggagatag gctcatttgg gaaggaagtc cagtttttgt
      301 ttagtgattg gggtaagata gttgttatca gatgagttta tactcttcga aaatcaaatt
      361 caaaccacgt caacgtcgcc ttgccgtata tatgtgactg acttcgtcag tcctatctac
      421 aacctcaaaa cagtgttttg agcagcctgc ggctagtttc ctagtttgct ctttgatttt
      481 cattgagtat tagggaaaag gagatgaata tgaaatttgg gaaacgtcac tatcgtccgc
      541 aagtggatca gatggactgc ggtgtagctt cattagccat ggtttttggc tactatggta
      601 gttattattt tttggctcac ttgcgagaat tggctaagac gaccatggat gggacgacgg
      661 ctttgggctt ggtcaaggtg gcagaggaga ttggttttga gacgcgagcc attaaggcga
      721 atatgacgct ttttgacttg ccggatttga cttttccttt tgttgcccat gtgcttaagg
      781 aagggaaatt gctccactac tatgtggtga ctgggcagga taaggatagc attcatattg
      841 ccgatccaga tcccggggtg aagttgacta aactgccacg tgagcgtttt gaggaagaat
      901 ggacaggagt gactcttttt atggcaccta gtccagacta taagcctcat aaggaacaaa
      961 aaaatggtct gctctctttt atccctatat tagtgaagca gcgtggcttg attgccaata
     1021 tcgttttggc aacactcttg gtaaccgtga ttaacattgt gggttcttat tatctgcagt
     1081 ctatcattga tacctatgtg ccagatcaga tgcgttcgac actagggatt atttctattg
     1141 ggctagtcat cgtctacatc ctccagcaaa tcttgtctta cgctcaggag tatctcttgc
     1201 ttgttttggg gcaacgcttg tcgattgacg tgattttgtc ctatatcaag catgtttttc
     1261 acctccctat gtccttcttt gcgacacgca ggacagggga gatcgtgtct cgttttacag
     1321 atgctaacag tatcatcgat gcgctggctt cgaccatcct ttcgattttc ctagatgtgt
     1381 caacggttgt cattatttcc cttgttctat tttcacaaaa taccaatctc tttttcatga
     1441 ctttattggc gcttcctatc tacacagtga ttatctttgc ctttatgaag ccgtttgaaa
     1501 agatgaatcg ggataccatg gaagccaatg cggttctgtc ttcttctatc attgaggaca
     1561 tcaacggtat tgagactatc aagtccttga ccagtgaaag tcagcgttac caaaaaattg
     1621 acaaggaatt tgtggattat ctgaagaaat cctttaccta tagtcgagca gagagtcagc
     1681 aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt cggcattctc tggatggggg
     1741 ctgttctggt catggatggc aagatgagtt tggggcagtt gattacctat aataccttgc
     1801 tggtttactt taccaatcct ttggaaaata tcatcaatct gcaaaccaag cttcagacag
     1861 cgcaggttgc caataaccgt ctaaatgaag tgtatctagt agcttctgag tttgaggaga
     1921 agaaaacagt tgaggatttg agcttgatga agggagatat gaccttcaag caggttcatt
     1981 acaagtatgg ctatggtcga gacgtcttgt cggatatcaa tttaaccgtt ccccaagggt
     2041 ctaaggtggc ttttgtgggg atttcagggt caggtaagac gactttggcc aagatgatgg
     2101 ttaattttta cgacccaagt caaggggaga ttagtctggg tggtgtcaat ctcaatcaga
     2161 ttgataaaaa agccctgcgc cagtacatca actatctgcc tcaacagccc tatgtcttta
     2221 acggaacgat tttggagaat cttcttttgg gagccaagga ggggacgaca caggaagata
     2281 tcttacgggc ggtcgaattg gcagagattc gagaggatat cgagcgcatg ccactgaatt
     2341 atcagacaga attgacttcg gatggggcag ggatttcagg tggtcaacgt cagagaatcg
     2401 ctttggcgcg tgctctcttg acagatgcgc cggtcttgat tttggatgag gcgactagca
     2461 gtttggatat tttgacagag aagcggattg tcgataatct cattgctttg gacaagacct
     2521 tgattttcat tgctcaccgc ttgactattg ctgagcggac agagaaggtg gttgtcttgg
     2581 atcagggcaa gattgtcgaa gaaggaaagc atgctgattt gcttgcacag ggaggctttt
     2641 acgcccattt ggtcaatagc tagaaagagg agaggatgaa accagaattt ttagaaagtg
     2701 cggagtttta taatcgtcgt taccataatt tttccagtag tgtgattgta cccatggccc
     2761 ttttgctcgt gtttttactt ggctttgcaa ctgttgcaga gaaggagatg agtttgtcca
     2821 ctagagctac tgtcgaaccc agtcgtatcc ttgcaaatat ccagtcaact agcaacaatc
     2881 gtattcttgt caatcatttg gaagaaaata agctggttaa gaagggggat cttttggttc
     2941 aataccaaga aggggcagag ggtgtccaag cggagtccta tgccagtcag ttggacatgc
     3001 taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag cctgcaagaa ggggagaacc
     3061 actttccaga ggaggataag tttggctacc aagccacctt tcgcgactac atcagtcaag
     3121 caggcagtct tagggctagt acatcgcaac aaaatgagac catcgcgtcc cagaatgcag
     3181 cagctagcca aacccaagcc gaaatcggca acctcatcag tcaaacagag gctaaaattc
     3241 gcgattacca gacagctaag tcagctattg aaacaggtac ttccttggcc ggtcagaatc
     3301 tagcctactc tctttaccag tcctacaagt ctcagggcga ggaaaatccc caaactaagg
     3361 ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt agaatctagt cttgctactt
     3421 accgtgtcca gtatgcaggt tcaggtaccc agcaagccta tgcgtcaggg ttaagcagtc
     3481 aattggaatc ccttaaatcc caacacttgg caaaggttgg tcaggaattg acccttctag
     3541 cccagaaaat cttggaggca gagtcaggta agaaggtaca gggaaatctt ttagacaagg
     3601 ggaaaattac ggcaagtgag gatggggtgc ttcatcttaa tcctgagacc agtgattcta
     3661 gcatggttgc agaaggtgcc ctactagccc aactttatcc atctttggaa agagaaggga
     3721 aagccaaact cacagcctat ctaagttcaa aagatgtagc aagaatcaag gtcggtgatt
     3781 ctgttcgcta tactacgact catgatgccg ggaatcaact ttttctagat tctactatta
     3841 caagtattga tgcgacagct actaagactg agaaagggaa tttctttaaa atcgaggcgg
     3901 agactaatct aacttcggag caggctgaaa aacttaggta cggggtggaa ggccgtctac
     3961 agatgattac gggcaagaaa agttatctac gttattattt ggatcaattt ttgaacaaag
     4021 agtaatgttc gtgtttttag agttaaataa tttttaaact gtgagaaaga ttcttcttgc
     4081 agttttttct ttacaatttt tgaaaaacat ctgctattta ttcggttaaa ttcttgtgtt
     4141 ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa aggcgaactt taaaatgtca
     4201 aaacaattga tctattcggg aaaagctaaa gatatctata caactgagga tgaaaatctt
     4261 attatttcaa cttacaagga ccaggcgact gctttcaatg gtgtcaagaa ggagcagatt
     4321 gcaggtaagg gagtcttgaa taatcagatt tcatctttta tttttgagaa attaaatgcg
     4381 gctggtgtgg cgactcactt tgtggagaaa ctttcagaca cggaacaact caataaaaag
     4441 gttaagatta ttcctttgga agtcgtgctc cgcaactata ctgctggttc cttttcaaaa
     4501 cgttttggtg tggatgaggg aatcgccttg gagactccga ttgtcgaatt ttactacaaa
     4561 aatgatgatt tggatgatcc atttatcaat gatgagcatg taaaattcct acagattgcg
     4621 gatgaccagc agattgccta cttgaaggaa gaaacgcgtc gtatcaatga actattgaaa
     4681 gtctggtttg ctgagattgg gcttaaattg attgacttta agctagagtt cggttttgac
     4741 aaggatggca agattatctt ggcagacgaa ttttcaccag ataactgccg cttgtgggat
     4801 gctgatggca accacatgga taaggatgtt ttccgtagag gattgggaga actaaccgac
     4861 gtttatgaga ttgtttggga aaagttgcag ggtttaaaat aacaacctca aggctgtttg
     4921 ggaatattgc aagagctgaa ataaaggaat aagaattgat ggataaacgt atttttgttg
     4981 aaaaaaaggc tgattttcag gtcaagtcag agagtttggt tagagagctc cagcacaact
     5041 tgggactgtc aagcttgaaa agtattcgta ttgtgcaagt atatgatgta tttgacttgg
     5101 ttgaggactt gtttgcacct gcagagaagc acattttctc tgagcaggta accgaccatg
     5161 ttttagatga agtatctgtg caggcggatc ttgctaacta tgctttcttt gccattgaaa
     5221 gtctaccagg gcagtttgac cagcgtgcag cttcgtcaca ggaagccttg cttttgttgg
     5281 gaagttcgag tgacgtgaca gtcaacacag cccaacttta cttggtgaat aaagatattg
     5341 atgcgactga gttggaagct gtcaaaaact acctgctcaa tccagttgat tctcgtttca
     5401 aggatatcac gatagggatt gccaagcagg agttttcaga gtcagacaag accattccaa
     5461 aattgccttt ctttgaaagc tatacagcag aagactttgc tcgctacaag gccgaacaag
     5521 ggatggccat ggaagtggat gatttgctct ttatccaaga ctactttaag tcaatcgggc
     5581 gcgtgccaac tgagactgaa ctcaaggttt tggacactta ctggtctgac cactgccgtc
     5641 atacgacttt tgagacagag ttgaaacaca tcgacttttc agcttctaaa tttcaaaagc
     5701 aattgcagtc aacctatgac aagtatattg ccatgcgcga ggaattaggt cggtctgaaa
     5761 aaccacaaac cttgatggat atggcgacta ttttcggtcg ttatgagcgt gctaatggac
     5821 gattggatga tatggaagtc tctgacgaaa tcaatgcctg ctcagttgaa attgaagtgg
     5881 acgttgatgg tgtcaaggaa ccttggctcc tcatgtttaa aaacgaaact cacaaccatc
     5941 caacagaaat tgagccattt ggtggagcgg ctacctgtat cggtggagct attcgtgatc
     6001 cgttgtcagg ccgttcctat gtttaccaag ccatgcgtat ttcaggtgct ggtgatatta
     6061 cagcaccgat ttcggaaact cgtgctggga aattgccaca acaagtcatt tctaaaacag
     6121 caactcatgg ttattcttca tatggtaacc agattgggct tgccacaacc tacgttcgtg
     6181 aatacttcca cccaggcttt gtagctaaac gtatggaact tggtgccgtt gttggtgcgg
     6241 ctcccaaggg caatgttgtc cgtgaaaaac ctgaagcagg tgatgtgatc atccttctcg
     6301 gaggtaaaac aggtcgtgat ggtgtcggtg gtgcgacggg ctcttctaag gttcaaacag
     6361 ttgagtctgt agagactgct ggtgctgagg ttcaaaaagg aaatgccatc gaagaacgca
     6421 agattcagcg cctcttccgt aatggcaatg tcactcgtct gatcaagaag tccaatgact
     6481 ttggggcagg cggtgtctgt gtggctatcg gtgaattggc agacggtctt gaaatcgacc
     6541 tcaacaaggt gcctcttaaa taccagggct tgaatggtac agaaattgcc atctctgaat
     6601 cacaagaacg gatggcggtc gtggttcgtc ctgaagatgt ggataccttc gttgccgaat
     6661 gtaacaaaga aaatattgat gctgttgtgg tggcgacagt aactgaaaaa ccaaatcttg
     6721 tcatgcactg gaatggtgag acaatcgttg acttggagcg tcgtttcctt gacatcaatg
     6781 gtgtgcgcgt ggttgtcgat gccaaagttg tggacaagga tgtcaaactc ccagaagagc
     6841 gtcaaacatc tgctgaaaca ctggaatcag atacccttac ggttctatct gacctcaacc
     6901 atgcaagtca aaaaggatta cagactatct ttgactgctc tgttggacgc tcaacggtta
     6961 atcacccact tggtggtcgt taccaactca caccaactga ggcatctgtg cagaaattgc
     7021 cagttcaaca cggtgtgact catactgcgt cggtcattgc tcaaggtttc aacccatatg
     7081 tagctgaatg gtctccatac cacggtgctg cttatgcggt tatcgaagca actgctcgtt
     7141 tggtggctgc tggtgccaac tggtccaagg ctcgtttctc ttaccaagaa tatttcgagc
     7201 gtatggacaa gcaagcagag cgttttggtc agccagtagc cgctcttcta ggctctattg
     7261 aagctcagat tcaacttggc ttgccatcta tcggtggtaa ggactccatg tctggtacct
     7321 ttgaagaatt gaccgttccg ccaaccttgg ttgcctttgg ggtgacgacg gcagatagcc
     7381 gtaaggtgct ctctccagaa tttaaagctg ttggggaaaa tatctactac atcccaggtc
     7441 aagccctctc tgcagagatt gattttgact tgattaagaa aaattttgct cagtttgaag
     7501 caagccaagc tgaccataaa gtgacatctg catcagctgt caaatacggt ggtgtagttg
     7561 aaagtttggc tcttgctacc tttggaaact atattggtgc agaggtgacc ttgcctgaac
     7621 ttaaaacagc tttgacagct caattaggcg gctttgtctt cacatctcct gaagaaattg
     7681 ctggagtaga gaagattgga caaacgaaag cagactttac actgactgtc aacggtgtga
     7741 agctagatgg acacaagctt gacagtgcat ttcaagggac attggaagaa gtttacccaa
     7801 cagaatttac ccaagcgaaa gaactagaag aagtaccagc tgtggcatca gatgttgtga
     7861 ttaaagccaa agaaaaggtt gaaaaacctg tggtttacat cccagtcttt ccaggaacca
     7921 actcagaata tgattcagct aaggccttcg aaaaagaagg tgcagaggtc aatttggtgc
     7981 cattcgtgac cttgaatgaa gaagctattg tcaagtcagt tgaaactatg gttgacaata
     8041 tcgacaagac taatattctc ttctttgctg gtggattctc ggctgcggat gaaccagatg
     8101 gttcagctaa gtttatcgtc aatatcctgc ttaatgaaaa agtgcgtgtg gctattgata
     8161 gctttatcgc ccgtggtggt ttgattatcg gtatttgtaa tggattccaa gccttagtca
     8221 aatcgggtct cctaccctac ggaaactttg aagatgctaa caatactagc ccaaccctct
     8281 tctacaatga tgccaaccaa cacgtggcca agatggtgga aactcgcatt gccaatacca
     8341 actcaccatg gttggctggt gtgcaagtgg gcgatatcca cgctattcct gtttcgcacg
     8401 gtgaagggaa gtttgtcgtg acggctgagg aatttgcaga gctccgtgac aatggacaaa
     8461 ttttcagcca atacgttgac tttaacggta aaccaagtat ggattctaag tacaatccga
     8521 atggttctgt ccatgccatc gaaggaatta ccagcaagaa tggtcaaatc atcggtaaga
     8581 tgggccactc agaacgttat gaggatggtc ttttccaaaa tatcccaggc aataaagacc
     8641 aacacctgtt cgcatcagcg gttaaacatt tcactggaaa ataagactta cagattttct
     8701 aatagatagt atcagtaatg taaaagtcat gtaaatctag ctcttgatga ttacaaatga
     8761 aaattaggta taaaaaatga catacgaagt aaaatctctt aatgaagaat gtggtgtttt
     8821 cggtatttgg ggacatccag atgctgctaa gttgacctat tttggactcc acagtcttca
     8881 acaccgtggt caggaggggg caggaatcct ctccaatgat caaggacaac tgaagcgcca
     8941 tcgtgacatg gggcttttat cagaagtttt cagaaatcca gccaatttgg ataaattgac
     9001 aggagctggt gcgattgggc atgtgcgtta tgcgactgct ggcgaagctt ctgttgataa
     9061 catccagccc ttcctcttcc gttttcacga tatgcagttt ggcttggctc ataatggaaa
     9121 tctgaccaat gcagcctctc tcaagaaaga actggaacaa agaggagcaa ttttcagcgc
     9181 gacttcggac tctgaaatct tggctcacct cattcgtcgc agtcataatc ctagcctgat
     9241 gggcaaaatc aaggaagcgc tcagccttgt caaaggtggt tttgcctata tcttgctgtt
     9301 tgaggacaag ttgattgcgg ctcttgaccc taatggcttt cgtccgcttt ctatcgggaa
     9361 aatggccaac ggagcggtgg ttgtttcctc tgaaacctgt gcttttgagg tcattggtgc
     9421 cgagtggatt cgtgatttga agccaggtga gattgtgatc attgatgacg agggcattca
     9481 gtatgacagc tatacagatg atactcagtt ggcgatttgt tctatggagt atatctactt
     9541 tgctcgccct gattctaata tccacggtgt caatgtccat acggcacgta agagaatggg
     9601 agcgcaattg gcgcgagaat ttaagcatga ggcagatatt gtagttggtg tgcccaattc
     9661 ttccctaagc gcggctatgg gatttgcgga agaatcaggc ttaccaaatg aaatgggtct
     9721 gatcaaaaac caatacaccc agcgaacttt tatccaaccg actcaagaat tgcgggagca
     9781 aggagtgcgg atgaaactgt ctgctgtttc gggtgttgtc aaaggcaaac gtgtggtcat
     9841 ggtggatgat tccattgtac gtggaacaac ctctcgtcgt atcgttcagc tcttgaaaga
     9901 agcgggtgcg actgaggttc acgttgccat tggaagtcct gcactagcgt atccatgttt
     9961 ctacgggatt gatatccaga cccgtcagga gctgattgca gccaatcata cggtcgaaga
    10021 aactcgccaa atcattggtg cggacagtct gacttatctt tcaattgatg gcttgattga
    10081 gtcgattggt atcgaaacag atgcgccgaa cggtggtctc tgtgtcgctt actttgacgg
    10141 tgactaccca acgcctctct atgactacga agaagactat cgtagaagtt tggaagaaaa
    10201 gaccagtttt tacaagtagg cgacagattc tccattaaag aaaaggaaaa aacaaatgac
    10261 aaataaaaat gcatatgccc agtcgggtgt ggatgttgaa gcgggttatg aagttgttga
    10321 acggattaaa aagcacgtgg cccgtacgga gcgtgcaggt gtcatgggag ctcttggtgg
    10381 ctttggtggt atgtttgacc tttccaagac tggggttaaa gaacccgtct tgatttcagg
    10441 gactgacggt gtcggaacca agctcatgtt ggctatcaag tacgacaagc acgataccat
    10501 cgggcaggac tgtgtggcca tgtgtgtcaa cgacatcatt gctgcaggtg cggaacccct
    10561 ctattttctc gactacgtag cgacagggaa gaatgaacca gctaagctag aacaagtggt
    10621 tgctggtgtg gcagaaggtt gtgtgcaggc tggtgctgcc ctcatcggtg gggaaacggc
    10681 tgaaatgccg ggcatgtacg gcgaagacga ctatgacttg gctggttttg cggtcggtgt
    10741 ggctgaaaaa tctcaaatca ttgacggttc aaaggtggta gagggagatg ttcttctcgg
    10801 acttgcttca agtgggattc actcaaatgg ttactctttg gttcgtcgtg tctttgcgga
    10861 ttacacaggt gaggaagtcc taccagaatt ggaaggcaag aaacttaagg aagttctact
    10921 tgagccgact cgtatctatg tcaaggctgt cttgccgctc atcaaagaag agttggtcaa
    10981 cggcattgcc cacatcacag gtggtggctt tatcgaaaat gtccctcgta tgtttgcaga
    11041 tgacctagct gctgaaattg atgaaagcaa agttccagtg cttccaattt tcaaagccct
    11101 tgaaaaatat ggtcagatta aacacgaaga aatgtttgaa atcttcaata tgggtgtggg
    11161 acttatgttg gcggtcagcc ctgaaaatgt agagcgtgta aaagaattgt tggatgaagc
    11221 agtctatgaa attggtcgca tcgtcaagaa agaaaacgaa agtgtcatta tcaaatgaaa
    11281 aaaatagcgg tttttgcctc tggtaatggc tcaaattttc aggtgattgc cgaagaattt
    11341 ccagtggagt ttgtcttttc agaccatcgt gatgcctatg tgcttgagcg tgcaaagcag
    11401 ctcggcgttc tgtcctatgc ttttgaactc aaggagtttg agagcaagac agactacgaa
    11461 gcagcccttg tcgaactctt ggaagaacac cagattgact tggtttgcct agcaggctac
    11521 atgaaaatcg ttggaccaac cttattgtcg gcttatgaag gtcggattgt caacattcat
    11581 ccagcctact tgccagaatt tccaggagct catgggattg aggatgcttg gaatgctggc
    11641 gtgggtcagt ctggtgtgac cattcactgg gtggattcgg gtgtggatac aggccaggtc
    11701 atcaaacagg ttcgtgtgcc acgactagct gatgatacca ttgacagatt tgaagctcgc
    11761 atccatgaag cagagtacag gttgtatccg gaagtagtga aggctctatt tacagattga
    11821 ctttttgatg attcatatga tatctttgat tttaaattgg agtcagtgtt tgttgaagac
    11881 ggcttcaaac ggaggtattt gtaatgttag aatctaaaaa aacaactcga tatgtatttt
    11941 atgtctatct gatgttatta acttggggaa tcttatttaa gtttgaaaca aatcctgaat
    12001 ttatagcatt tttcttagct ccaaggtata tcaattggat tccattttca gaaccactaa
    12061 tagtcgatgg aaaaattgtt tttgctgaaa tgttatttaa tctgatttcc tttattccat
    12121 taggtgtttg tttccctttg ataaaaacta atttatctag tttaagaata gtcgggacag
    12181 gtttcttgat tagtttattg tttgagtgct tacagtatat tttagcaata ggtataacag
    12241 atataacgga tttgacttta aatacgctag gtgtctgtgt aggcttactg atttatcaaa
    12301 tttttataag agtgttcaaa tcacagacta gaaaatggat caatatctta ggtatgctta
    12361 gccttggttt tgcttatctt gttttactgt tactgcattt aattggtgtt taactaatga
    12421 ttaaaaagga gaatataatg actaaacgcg tcttaatcag cgtctcagac aaagcgggca
    12481 ttgttgaatt tgcccaagaa ctcaaaaaac ttggttggga gattatctca acaggtggaa
    12541 ctaaggttgc ccttgataat gctggggtgg ataccattgc tatcgatgat gtgactggtt
    12601 tcccagaaat gatggacggt cgtgtgaaga ccctccaccc aaatatccac ggagggcttc
    12661 tcgctcgtcg tgacttggat agccacttgg aagcggctaa ggacaacaag attgagctca
    12721 ttgaccttgt ggtggtcaac ctttacccat ttaaggaaac tatccttaaa ccagatgtga
    12781 cttatgctga tgcagttgaa aatatcgata ttggtgggcc gtctatgctt cgttcagcag
    12841 cgaaaaatca tgccagtgtt acagttgtgg tagatcctgc tgactacgct gtggttttgg
    12901 acgaattggc agcaaacggc gaaacctctt atgaaactcg ccaacgttta gcagccaaag
    12961 tatttcgtca cacagcggct tatgacgcct tgattgcaga atacttcaca gctcaagtgg
    13021 gtgaaagcaa gcctgaaaaa ctcactttga cttatgacct caagcaacca atgcgttacg
    13081 gtgagaatcc tcaacaagac gcggactttt accagaaagc tttgcctaca gattactcta
    13141 ttgcttcagc caaacagctc aacgggaaag aattgtcatt taataatatc cgtgatgcag
    13201 atgctgctat ccgtatcatc cgtgacttca aagatagtcc aaccgttgtg gctctcaaac
    13261 acatgaatcc atgtggaatt ggtcaagctg atgacatcga gactgcttgg gactacgctt
    13321 atgagtctga cccagtatct atctttggtg ggattgtcgt cctcaaccgt gaggtggatg
    13381 ctgcgacagc tgagaagatg cacggcgttt tcctcgaaat catcattgca ccaagctata
    13441 cggatgaagc gctagctatt ttgatcaata aaaagaaaaa cttgcgtatc cttgccttgc
    13501 catttaatgc tcaagaggct agcgaagtgg aagcagaata cacaggtgta gtcggtggac
    13561 ttctcgtgca aaatcaagac gtggtcaagg aaagcccagc tgactggcaa gtggtgacca
    13621 aacgtcagcc aactgagaca gaagcgactg ctcttgagtt cgcttggaag gctatcaagt
    13681 acgtcaaatc aaatggtatt atcgtgacca acgaccacat gacacttggt gttggtccag
    13741 gtcaaaccaa ccgtgtggct tctgttcgcc ttgccattga ccaagccaaa gatcgtctga
    13801 acggggcggt ccttgcttca gatgccttct tcccatttgc ggataacgtg gaagaaatcg
    13861 ccaaagcagg aattaaggcc atcatccagc ccggtggctc tgtccgtgac caagaattca
    13921 tcgaagcagc ggataaatac ggcttgacta tggtctttac aggtgtgaga cattttagac
    13981 attaagaaga taaaagggaa gaaaacagtt tctttccttt tttggcttaa aatgctaact
    14041 gaaacaagat taaaacgaac ttttttgata taatgttggt aaataattcg caaaagaggt
    14101 tgaggaatga aactgcttgt tgtcggttct ggtggtcgtg agcatgcgat tgctaaaaag
    14161 ttacttgaat caaaagacgt ggaaaaagtc tttgtagctc ctgggaatga cggtatgact
    14221 ctggatggtc tggaattggt aaatatatct atttccgaac attataaatt gattgacttc
    14281 gcaaaggcca acgatattgc ttggaccttt atcggtccag atgacgccct tgcggctggt
    14341 atcgtggatg atttccatgc agctggtctc aaagcctttg gtccgacaag attggcagcg
    14401 gagctggagt ggtccaagga tttcgccaag gaaatcatgg tcaaatatgg tgtttcgaca
    14461 gcagcctatg gcacattttc agatttcgag gaagcaaagg cctatatcga aaagcatggt
    14521 gcgcctatcg tagtcaaggc ggatggcttg gcgcttggga agggtgtcgt cgttgctgag
    14581 acggttgagc aagcggtcga agccgctcat gagatgcttt tggacaataa atttggtgac
    14641 tcaggtgcgc gcgtggttat tgaggaattc cttgaaggag aggaattttc actctttgcc
    14701 tttgtcaatg gtgataagtt ctacatcatg ccaacggctc aggaccacaa acgtgcctac
    14761 gatggcgaca aagggcctaa cacgggtggt atgggtgcct atgcgccagt tccacactta
    14821 ccacagagtg tagttgatac agcggttgac accattgtca agccagttct agaagggatg
    14881 attaaagaag gtcgccctta tctgggagtt ctttacgcag ggcttatcct gacagctgat
    14941 ggaccgaaag tcattgagtt caacgctcgg ttcggagatc cagaaactca gattatcttg
    15001 cctcgcttga cctctgactt tgctcaaaat atcacagata tcctggatag caaggagcca
    15061 aatatcatgt ggacggacaa gggtgtgact ctgggtgtgg ttgtcgcatc caagggctac
    15121 ccgctagact atgaaagggg cgttgagttg ccagccaaga cagaaggcga tgtcatcacc
    15181 tactatgcag gggctaagtt tgcggaaaat agcagagcac tgctctcaaa cggcggacga
    15241 gtttatatgc tcgttaccac agcagatacc gtcaaagaag cccaagccag catctaccaa
    15301 gaactatccc aacaaaaaat agaaggactc ttctaccgaa cagatatcgg aagcaaggca
    15361 attaagtaaa gatataagaa taacgcgccg tagtcgccaa acacgataat ggtcgtcgtg
    15421 gtgaaaagac cagaacagtg aatgttctgg tcagggggaa acttggagac cgtaggctca
    15481 aagtttagga atgaaaccga aggtttgctt ccgcctccat cacctaagac cattatcaaa
    15541 aagaaaaata aaaattcaca aaatacgtta atggtcgtat ggtttgcgag cgttagcgag
    15601 ctaatataga acaatcaccg ccgttgtgaa agaacgattg gatgataatc caatcgttca
    15661 gggaaattgg aagaccttgg gtttccaatt taggcatgag acacctttgg tggctgctgc
    15721 cgtccctcac aagctaaggt gattgttgaa aaagaggaaa aaggagaaga aatgaaacca
    15781 gtaatttcca tcatcatggg ctcaaaatcc gactgggcaa ccatgcaaaa aacagcagaa
    15841 gtcctagacc gcttcggtgt agcctacgaa aagaaagttg tttccgcaca ccgtacacca
    15901 gacctcatgt tcaaacatgc agaagaagcc cgtagtcgtg gcatcaagat catcatcgca
    15961 ggtgctggtg gcgcagcgca tttgccaggc atggtagctg ccaaaacaac ccttccagtc
    16021 attggtgtgc cagtcaagtc tcgtgctctt agtggagtgg attcactcta ttctatcgtt
    16081 cagatgccgg gtggggtgcc tgttgcgacc atggctatcg gtgaagctgg agcgactaac
    16141 gcagctctct ttgccctccg tctcctctct gtagaagata agtccattgc ggatgcactt
    16201 gccaactttg ctgaagaaca aggaaaaatc gcagaggagt cgtcaaatga gctcatctaa
    16261 aacaatcgga attatcggtg gcggtcaact gggtcagatg atggccattt ctgctatcta
    16321 catgggccac aaggttatcg cgctggatcc tgcggcggat tgcccggcct ctcgcgtggc
    16381 ggaaatcatt gtggcacctt ataacgatgt ggacgccctc cgtcagttgg cagaccgttg
    16441 cgatgtcctc acttatgagt ttgaaaatgt cgacgctgac ggtttggatg ccgttatcaa
    16501 ggatggacaa ctccctcaag gaacagatct gctccgcatt tcgcaaaatc gtatttttga
    16561 aaaggacttt ttgtcaaaca aggctcaagt cactgtggca ccctacaagg tcgtgacttc
    16621 tagcctagac ttggcagata tcgacttgtc gaaaaactat gtcctcaaga ctgcgactgg
    16681 tggctacgat ggtcatggac aaaaggttat tcgttcagaa gcagacttgg aagcagccta
    16741 tgcgctagca gactcagcag actgcgtctt ggaagaattt gtcaactttg accttgagat
    16801 ttctgtcatc gtgtcaggaa atggcaagga ggtgacgttt ttcccagttc aggaaaatat
    16861 ccaccgcaac aatatcctgt ctaagaccat cgtaccagcc cgcatttctg aaagtctagt
    16921 agacaaggct aaagctatgg cagtgcgaat cgcagaacaa ctcaacttgt ctggaactct
    16981 ctgtgtggaa atgtttgcga ctgatgatga cgttattgtc aatgaaatcg ccccacgacc
    17041 acataactct gggcactatt ctattgaagc ctgtgatttc tctcagtttg acacccatat
    17101 tctcggtgtt ctcggagcac cattaccagt catcaaactc catgcgcctg ccgtcatgct
    17161 taacgttctc ggccagcatg ttgaggccgc tgaaaaatat gtcacagaaa attcaagcgc
    17221 ccacctccac atgtatggta aaatagaagc gaagcacaac cgcaagatgg gacatgtgac
    17281 tttgattagt aatgaaccag atagtgtggg agagttttag gggagagggg tctaaagaaa
    17341 aaatgtttga aaaaattaaa ggaattaata taaaatcagg tatttttgaa gatgaaacaa
    17401 aattagaact ctttgaagga aattttgagg gaacaaatcc agtacaaaac gatagggcaa
    17461 gtttattgtt tggaaggaat ggttcaggga aaagtaccat tgctcgagga ataaatcaat
    17521 taaaaaatgg tgagattgga acagatagag tatcatttat tgataaaaat aataataata
    17581 ttgtacttag tgatactgaa cgaaaatcga tatttgtttt caatgaacac tatgttgatc
    17641 agaaagtcaa aattgctcaa gaagggttag atacaattgt aattttaggt gaacaggttg
    17701 atatcgatga ggaactggat agattaagaa cgcagttatc agaatcacaa attgaatctc
    17761 aagattatta tgctgaatat gaagaatatt tagatgaaaa aaatgaaaaa tctccagatt
    17821 tttggaaaaa ggaaatgaca gattctttga aaggagtagg caactgggcc gaaagagata
    17881 gggagataaa aggaaatcga gctgcatctc ccgttcataa taataccttt caaaattttg
    17941 ttgatttaca accaatctta gataaaaatg agttagaggt tgaattcaac aataaaaaag
    18001 ctagatattt ttcaataaga gattccgcag ttactataaa taatgaacta tctttacctg
    18061 atattaattt tgatagcaat gaattatcaa ctttattgtc tgaaaaaatc gaagaacctg
    18121 agttgaattc tagggataag tatttactga cattattatc tgattccaca aaaggtgaac
    18181 gtcatttgag agaagttaaa gatttctttg aagatgaaca tcaaaaaaaa tgtcctttct
    18241 gtactcaatc agtatcagaa gatgttaaag ttgaactcac gaatggcata actaaactac
    18301 tatcaagagc agtcgaagaa caccaatccg cattgagagg taaaaaaatt gatgagatta
    18361 atcaagattt ttctggttat gaacagatag atccaatatt aattcagtct tatcaaaata
    18421 gtattaatgc tttgaatgct aaatttaacg aaattaactc aataatcgat aagaagattg
    18481 ataatccata taatatagtt gagttaccta atataagctt ttctcaagaa ttaagtcaag
    18541 cagagcatga tattgaaaaa attaaccaag caattatcaa acataattca gaaatctcgg
    18601 gtattcaaaa gttaaaagta gatctccttc aaataaataa tgaattagct ttttatgaaa
    18661 ttcaagatgc atacaaaaag tttcaagaga aaacaaataa aaaagcaatt tgtgaaaata
    18721 attataataa cagtagtaaa cgag
//
LOCUS       DAVKFH010000031        18370 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKFH010000031 DAVKFH010000000
VERSION     DAVKFH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.031"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            210..491
                     /locus_tag="VW006_001277"
     CDS             210..491
                     /locus_tag="VW006_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9380130.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            623..1567
                     /gene="manA"
                     /locus_tag="VW006_001278"
     CDS             623..1567
                     /gene="manA"
                     /locus_tag="VW006_001278"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9380131.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1589..3064)
                     /locus_tag="VW006_001279"
     CDS             complement(1589..3064)
                     /locus_tag="VW006_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU9380132.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3230..3394
                     /locus_tag="VW006_001280"
     CDS             3230..3394
                     /locus_tag="VW006_001280"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9380133.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3395..3901
                     /locus_tag="VW006_001281"
     CDS             3395..3901
                     /locus_tag="VW006_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEU9380134.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(3921..4376)
                     /locus_tag="VW006_001282"
     CDS             complement(3921..4376)
                     /locus_tag="VW006_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9380135.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4396..5571)
                     /locus_tag="VW006_001283"
     CDS             complement(4396..5571)
                     /locus_tag="VW006_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9380136.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPDLHFTGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5630..6475)
                     /locus_tag="VW006_001284"
     CDS             complement(5630..6475)
                     /locus_tag="VW006_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9380137.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6600..7157
                     /locus_tag="VW006_001285"
     CDS             6600..7157
                     /locus_tag="VW006_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9380138.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7176..7643
                     /locus_tag="VW006_001286"
     CDS             7176..7643
                     /locus_tag="VW006_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9380139.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7732..8361
                     /gene="upp"
                     /locus_tag="VW006_001287"
     CDS             7732..8361
                     /gene="upp"
                     /locus_tag="VW006_001287"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9380140.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8535..9125
                     /gene="clpP"
                     /locus_tag="VW006_001288"
     CDS             8535..9125
                     /gene="clpP"
                     /locus_tag="VW006_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9380141.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9204..9452
                     /locus_tag="VW006_001289"
     CDS             9204..9452
                     /locus_tag="VW006_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9380142.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9553..10713
                     /locus_tag="VW006_001290"
     CDS             9553..10713
                     /locus_tag="VW006_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9380143.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10981..11850
                     /locus_tag="VW006_001291"
     CDS             10981..11850
                     /locus_tag="VW006_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9380144.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11854..12810
                     /locus_tag="VW006_001292"
     CDS             11854..12810
                     /locus_tag="VW006_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9380145.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12810..13574
                     /locus_tag="VW006_001293"
     CDS             12810..13574
                     /locus_tag="VW006_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380146.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13574..14284
                     /locus_tag="VW006_001294"
     CDS             13574..14284
                     /locus_tag="VW006_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380147.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGQIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14698..15354
                     /locus_tag="VW006_001295"
     CDS             14698..15354
                     /locus_tag="VW006_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9380148.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15462..16557
                     /gene="prfB"
                     /locus_tag="VW006_001296"
     CDS             join(15462..15533,15535..16557)
                     /gene="prfB"
                     /locus_tag="VW006_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9380149.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16575..17267
                     /gene="ftsE"
                     /locus_tag="VW006_001297"
     CDS             16575..17267
                     /gene="ftsE"
                     /locus_tag="VW006_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9380150.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17260..18186
                     /locus_tag="VW006_001298"
     CDS             17260..18186
                     /locus_tag="VW006_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380151.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
BASE COUNT         5632 a         3437 c         4002 g         5299 t
ORIGIN      
        1 aatcgatttg actgtcctga tcgatttgcc ctgttcttgt ttcgttttac tatagaacga
       61 tttgaaggcg tttatgatat ttagctgtac gagagtcttt aaaaagtgtt ttgatggttt
      121 ggatttcttc tttagttgat ttcatatcac tattatataa tgctttttga ttttagtata
      181 gtattatcgt agaaaatgga ctcggtgcta tggatacacc ggatgagaat ggttatgtag
      241 cagatgacta tcggattact tacttagagg cccacatcaa ggccatgcga gatgccattt
      301 acaaagacgg ggttgacttg cttggttata cgacatgggg ctgtatcgat tcggtttcag
      361 ctggcacagg ggaaatgaat aagcgctatg gttttatcta tgtagatcga gataatgtag
      421 gaaatgggac tctcaaacgt agcaagaaga agtccttcta ttggtacatg tcatttatag
      481 caatggtgta agcatcgagt agagcacatt tgtttactat ttttataaaa tcttctccct
      541 gctttataag atgtaaaaaa gagttcaatt cgaactcttt ttttgctata atgaggggag
      601 aaaaatcaga caggagatta agatgtcaga accattattt ttacaatcag ttatgcaaga
      661 aaaaatctgg ggtggtacca agctacgtga tgagtttggc tacgacatcc caagtgaaaa
      721 agtcggagag tactgggcta tctcagctca tctaaatggt gtttctaaag ttgccaatgg
      781 tcgttacgag ggaacagatc ttgctacttt gtatgcggaa caccgtgaat tatttggcaa
      841 tcgtcctgag cctgtatttc cgcttttgac taagattttg gatgctaacg actggctcag
      901 cgttcaagtt cacccagacg atgcttatgg actagagcat gaaggcgaac ttggaaaaac
      961 agaatgctgg tacatcatcg ctgcggatga aggttcagag attatctacg gccacaatgc
     1021 caagtcaaaa gaagaactcc gccagcaaat cgaggataag aactgggatg cgctgttgac
     1081 aaaagtgcca gttaaagcag gagatttctt ctatgtacca agcggtacca tgcacgctat
     1141 cggggcgggt atcttgattc ttgaaaccca gcagtctagc gataccactt accgtgtcta
     1201 tgactttgac cgtaaggatg acaagggcaa tttacgtgaa cttcaccttg aaaaatctat
     1261 cgatgtcttg aacattggtg aacccgcaaa tagccgtcca gtgacaatca aagcagatga
     1321 tctacgttcc actctccttg tatccaatga tttcttcgca gtttacaaat gggaaattac
     1381 tggaaaagtt aactttgaaa agacagctga ctacagcttg ttgagtgtct tggctggtca
     1441 aggtcaacta actgttgacg ggaaaaacta tccaatccaa aaagggagac actttatcct
     1501 accaagtgat gttgaagctt ggactctgga agggcaagat ttagaattaa ttgttagtca
     1561 tccataaaaa agaaagggcc tgagttcatt actcaagtcc tttttgatta cataaattgg
     1621 gcgatgaaga ccacgatgat gattggaatg acggaacgaa gaaacgaaga aggaagagcc
     1681 agacttggaa cagtccttgt ttccatgctc tttcatcgag atggagttcc tccattgcaa
     1741 gagccttttt aaagatatag cctgtaaaaa gtgaaaggta gagagctcca aatggcatga
     1801 ggagattggg aaccaagaag ttcatagcgt caaagaaggt cttaccaaaa atgtgaacat
     1861 ccgccatgac accgtaagat agggctgaag gaatgccaaa gacaaaggtc aaaattccta
     1921 aaataacact ccatttggca cgtttgctgt tatcctggtt ggtgatattg cccacattga
     1981 tctccagcat cacgacagaa gaagtgacag tcgcaaagag gaagaggacg tagaaaatgg
     2041 ttccaaaagg catcttgtca aagagttgag gcaagacgat aaagagcaag ctcggtcccc
     2101 cttcagactg gatattgaag ggggaccgag cttggaaaat ggctagacct gccatgatgg
     2161 atatcgagat attcatggct acgatggaga ttcctgactg gactagattg gttttcttgt
     2221 ccaagtaaga agcataggtc aacatgactg taacccctag tgagagggca aagaaagatt
     2281 gtcccagagc atagaggaga ccagcgctag tcagttttga aaagtctggt ttgaggaagt
     2341 aaagaacccc ttccatggca tttggcaaac tgagagagcg accgatgata acaacaaaga
     2401 tgataaagag caggggcatc atgactttcg aagctctttc aatccctttt tgaaccccac
     2461 gtgatacaat aaagatattt aataagataa aggctgcttg agctcctagg gcaatggctg
     2521 gatttgaaat gattgaagta aataactgag cataatcacc cgttccacca agttggaaca
     2581 atttcccaaa ctcaatacct agatagacta gaatccatcc tccgataaca ctgtaaaaag
     2641 ataagaggat aaagagggca aaggcgccaa tccaaccgat aaagttgtac ttgttattct
     2701 tgcccagttt tccaaaggtt ttgatagcgg aaacgccagc actacggcca agggcaaact
     2761 cagccagcag gagagggaaa ccgattaaaa tagtggaaat gagaaagatt agtaaaaagc
     2821 ctccaccgcc attagcagca gtcatgtagg gaaacttcca aacggaacca agcccgatga
     2881 ccgagccagc agatgctaga ataaagccaa gtttcgaccc ccattgtgat ttttctgaca
     2941 tagttaaact ctgatctcct ttttcaaaat aagaaaaagc tacttgagtt gtcaaatagc
     3001 cttctgtttg attgatagac ttgactatcc tattatgttt tctaaggtct gtcaagaaaa
     3061 caattttcaa gttttcacac ctttctcaaa aacgcttgac tctgacctaa ggggaggggt
     3121 tatactatca ttgtaaggag gaaatcatgt accatataaa agaagctgcg cagctttcag
     3181 gtgtctctgt caagaccctg ccctgcatca ctatgacaag ataggactct tggtcccctt
     3241 aaagtcggaa aacggctatc gaacctatag tcaagaggat ttggaacgcc ttcaggtcat
     3301 tctttactac aaatatctag gcttttcttt agagaaaata gcagaactgt taaaggaaga
     3361 aaggacagat ttattgctcc atttgactag gtagttggac tatctaactc gcgaaaggca
     3421 acatctggat accttgattt ccaccttgca aaaaactatt caagaacaaa aaggagaaag
     3481 aaaaatgacc attgaggaaa aattcacggg atttagctat caagacaatc aaaaatacca
     3541 ccaagaagcg gtagagaaat atggtcaaga agtcatggga caagcgctcg aacgccaaaa
     3601 aggtcacgaa gacgaggcta cggccgcctt taaccaagtc tttcaaactt tggcacaaaa
     3661 tcttcaagtt ggtttacctg caacagcaac cgaaaaccag gagcaagcag ccaagctctt
     3721 gcaagccatt cgcacttatg gatttgactg ctctattgag gtattcggtc atatcggtaa
     3781 aggttacgtc tacaacccag agtttaagga aaacattgac aagtttggtt ctgaaacagc
     3841 ccagtacacg tcagatgcca ttgcggctta cgttcagaca aatgcagaaa aaataggcta
     3901 ggaatttcct agcctatttt ttacttcaaa tcataaagcc agtcgtcacc gtttttgtag
     3961 taaaagaatt cactgagatc ttcttctaga aacacacgaa gcatatcaga catatcatcg
     4021 gttgcaagtt ttagatgaga aagattttca aagtcctccc accaaacttt cccttcgtct
     4081 gaagactgga gttcaccagt aaagtgttct gtcttgtaaa aaaggacgac ataacgataa
     4141 tccttgtcgt cataccagtt tttgatacca cagagttggg gtttggaaat gatcagacca
     4201 gtttcttctt tcacttcacg aatgacagca tcgacaaagg attcgccacg ttcaacatga
     4261 ccaccaggaa aagtaatgcc agaccagtcg ggactaactc ggtcttggac caggacctta
     4321 tctccgtttt taatcataca catgttaaca aattcgactg cctctcttct gttcattctt
     4381 cacaaccttt aatctttaat cataatgcag acttcccgcc acccagccgg tacagagggc
     4441 agaagtgatg ttaaagccac ccgtgtgggc attgatatcc ataacttcgc ctgtaaagtg
     4501 gaggtcaggt accagcttac tttcaagggt tttaggattg atttccttga gactgactcc
     4561 acccttggta acaaaggact ttgcaaggga catttttcca gttacaggaa ttttaagttc
     4621 tttaatggac tggacaagtt gttctcgttc cttttcagtc agttgtttga ctttttcagg
     4681 atatccttgt acaaaaaatt cggccaagcg ttctggtaac aaggttttta aagcgttttt
     4741 caaggatttt tcccgatttt cttctagaaa tgtaaccaag tccttctcag aaagttgagg
     4801 caaaacatcg agtgagagaa cctccccacc tttgacaaag ctagacatgc gtagggcagc
     4861 aggacctgac aaaccaaagt gggtaaagag taaatcatga gtgatgacat gcttaccata
     4921 acttagggtc acatcgtcca gagaaatccc ttgtaaggct ttatgtggaa aatctgttaa
     4981 taaaggactt tcagcagcct caagatcggt gatggtatgc ttaaaatggc gagcaatctc
     5041 gtgaccaaaa ccagtcgaac cagtcgaagg ataagactta ccacctgttg tgacaatgag
     5101 tttctcacaa gtgaaggttt gatccgctga cttaaggaca aactggtcat ctactttttt
     5161 aacagaaacg atttctattt gagtagcaac ttgaccacct agttcggtga ttttcttttc
     5221 caaagcttcg ataatagtcc gagacttgtc actggctgga aagacgcgtc cgtggtcttc
     5281 gaccttaagt ttaacaccat tttctgtaaa aaagttgatg atgtcatgat tatcgaactg
     5341 ggagaaaaca ctgtaaagaa agcgtccgtt tccaggaatt ccagctagca ggttgtctaa
     5401 gctaccattg ttggtcacat tgcaacgtcc cccaccagtc ccagctaatt tttttccaag
     5461 tttccgattt ttttcgatga ggagggtttt ctgtccataa aagctactgg aaatcgtagc
     5521 catcatacca gcaggtcccc caccgatgac aatagtatca aaatgtttca tagctctatt
     5581 gtaccacaaa aaaacaagag atgatggtca cctcttgtca agaatgcaat taatcaattt
     5641 catagcccat cagcaaaccg ccctcttctg catagaaact gcagagacca gaggttggta
     5701 gaattttaat atccgcttgt gggaaggttt cacggattcg ctctgagagc tgttgacaac
     5761 atttttcgtt attgcgttgg gccatgacaa tacggccacc agcatatcca gcttttacta
     5821 actcatcata ggcagcttga actgatttct ttgatcccct tgctttttgt agcaattcga
     5881 gagttccagt ttcactagct tttccgacca tacgaatgtt gagaaggcca acgaccgtac
     5941 cgataagctt gctcaaacgg ccgttcttca ccaagttatc gactttggct aggacaaaga
     6001 gcaacttagt tttttcttga taggcagtga tagcttcaac cacttcttca aaagacaagc
     6061 cctggtcaat caagtcattc aatttttcta cgagtaggtc aacttcacca ccagcagata
     6121 aactatcaat cacatgaatc ttagtgtcag gatggtcttc tagataaata ttctttgcta
     6181 gttgagcact attgtgactg ccagaaaggg tacctgtgat ggttactagg aaaatgtttt
     6241 tggcaccttc aaatgctcgc aaatagtcat ctgggcttgg acaagccgat tttgaagctt
     6301 ctgcagttgc atacatggtt tccatcattt ggtcaatatc gagactggcg tcatcaacaa
     6361 agacctgatc agctacttga atggttaagg ggacacttac aaaggttgtg ttaatagctg
     6421 gtgttggcag ttgacgataa tcacaaccag agtcagcaat aattttccaa gtcatagaaa
     6481 ttctccatct ttgtcagtta tacattgaca aaggttctgt ctttttttac aattatatca
     6541 tgaaaaccct tgaaacaaaa gcatcatccg tctgttgtga caagtagaaa gaaaatgtta
     6601 tgtctgaacg tagaatctct gaaaagtctc ttgaaaatct cagaaaatca aaccaagaat
     6661 ccaatttatt aaccagagaa gccattgaaa cagccctctt gcaactcttg gaaaaaaagg
     6721 agctgaccaa gattagtatt tctgaattgg tcaaacgtgc aggtgtttcg cgtgcggcct
     6781 tttatcgcaa ttatgactcc aaagaggaaa ttttagagag cgtctttaaa cgaactgtcc
     6841 acaatattat ggaacagctg catcattacg atttaaagac agacctttat ttggtttggg
     6901 ttcacctttt ccgggaggcc agaaaggaag ctagagtgat tcaattggcc ttggattacc
     6961 atctggaaaa aatctttgtc caagccatgc aggaatttct agaaaaatac catgggaaat
     7021 caaaaggtgt cagctcttat cttcattcct tctggagctc agccatcgtc tctgtccttc
     7081 taaaatggat caaggatggc atgaaggtac cagctgaaaa gattgcagat ttaggtttac
     7141 cattttttaa aaaatagaga aaaaggagaa aagagatgac tgaaaaaaga ctggcctggg
     7201 atgagtattt tgcagcccaa gccctactaa ttgcgaatcg ttccacttgt aaacgtgcta
     7261 aagtgggcgc gattctggta aaagataata aggttatttc cactggttac aatggttcgg
     7321 tgtcagggac tgagcattgt attgatcacg aatgtttggt cattgaaggc cactgtgttc
     7381 gcacccttca cgctgaggtc aatgctatcc ttcaaggtgc tgagcgtggt gttcccaaag
     7441 gctttacagc ctatgtaacc cattttcctt gtctcaactg tacaaaacaa ttgcttcagg
     7501 tcggctgcaa gcgcgtggtt tatatcaacc agtaccgaat ggacgactac gcccaatacc
     7561 tttatcaaga aaaagggaca gaattgaccc atttaccact tgagacagta cagacagctc
     7621 ttaaagaggc tgatctaatg taaaaattat caaaaataaa tggcttagaa agatttttaa
     7681 accgtttttt ggtataataa gaagaataaa ttgaaagaag gaattccaaa aatgggaaaa
     7741 attgaagtta ttaatcaccc actgattcaa cacaaattgt caatcttgcg tcgtacagat
     7801 acttctacaa aagcttttcg tgagctagta gatgagattg caatgttgat ggggtatgaa
     7861 gtacttcgtg atcttccact agaagatgtg gaaatcgaaa caccaattac aaaaacagtt
     7921 caaaaacaat tggcaggtaa gaaattggcc atcgtcccaa tcttgcgtgc aggtatcggg
     7981 atggttgatg gtctcttgag cttggttcca gctgctaaag ttggccacat cggtatgtac
     8041 cgtgatgaag aaacacttca accagttgaa tacttggtga aattgcctga ggacattgac
     8101 caacgtcaaa tttttatagt agacccaatg ttggcaacag gtggctcagc aatcttggct
     8161 gttgattctc ttaaaaaacg tggcgcatca aatatcaaat ttgtctgcct tgtatctgct
     8221 ccagagggtg taaaagccct tcaagaagct catccagatg tagaaatctt tacagcagcc
     8281 ttggatgaac gtttgaacga acacggttat atcgttccag gtcttggaga tgctggagac
     8341 cgcttgttcg gtacaaaata agatcgaaaa gaagaatgac ttggaaaagt atttccagtc
     8401 acgaaaggag gttgggtttt tgtttctgtc taatgaaagc agagcaaaaa tttgaccttt
     8461 tttgaccaag atattataat agtcttatct tcagtcattt gaccaacaaa attaaaactc
     8521 aaaaggagaa atgaatgatt cctgtagtta ttgaacaaac aagccgtgga gaacgttctt
     8581 acgatattta ctcacgtctt ctcaaagacc gcatcattat gctgacaggt ccggttgaag
     8641 acaatatggc taactctgtt attgcccaat tgcttttctt ggatgcccaa gatagtacaa
     8701 aagatattta cctttatgtc aatacaccag gtggttctgt ttcagctggt ttggcaatcg
     8761 tagataccat gaactttatc aaggcagatg tccaaaccat tgttatggga atggctgcat
     8821 ctatgggaac agtcatcgca tcaagtggag caaaaggcaa acgtttcatg cttccaaatg
     8881 ctgaatacat gattcaccaa ccaatgggcg gtacaggtgg tggtacccaa caaactgata
     8941 tggctatcgc tgcagaacac ttgctcaaaa ctcgtaatac cttggaaaaa atcttggctg
     9001 aaaattcagg tcagtcaatg gaaaaagtcc atgcagatgc agaacgtgat aactggatga
     9061 gcgcccagga aacacttgaa tatggcttta ttgatgaaat tatggccaac aattcattga
     9121 actaatgatg atagaaggca aactcgactg ggtttgcttt ttttggtata atagggagag
     9181 atttcttaga aagaggattt atcatgtttg aaaaagtcaa tcgctctggc ttgattatct
     9241 atctttacta taatcgtgat gccaaaaaac tgcaggatta tggagatatt acctatcatt
     9301 ccaagaaaca tcgttactta caactctatg ttccaactca agaagtggag caattggtcg
     9361 gacgcttgag caaggaaaaa tttattaaaa aagttagggt ttgtcatatc caagagttgg
     9421 aaacaccctt tgtgggcaat ctttatcgat aggaaaacgt tatcatcgaa aaagttcaag
     9481 gaaaacattg acaattttct gataattcgg tatattctta acagactatt taagaaataa
     9541 ggagacaaaa agatgaagaa aaaatttgcc ctatcgtttg tggcgcttgc aagtgtagca
     9601 cttcttgcag cctgtggaga agtgaagtct ggagcagtca acactgctgg taactcagta
     9661 gaggaaaaga caattaaaat cgggtttaac tttgaagaat caggttcttt agctgcatac
     9721 ggaacagctg aacaaaaagg tgcccaattg gctgttgatg aaatcaatgc cgcaggtggt
     9781 atcgatggaa aacaaatcga agtagtcgat aaagataata agtctgaaac agctgaggct
     9841 gcttcagtta caactaacct tgtaacccaa tctaaagtat cagcagtcgt aggacctgca
     9901 acatctggtg cgactgcagc tgcggtagcg aacgctacaa aagcaggtgt tcccttgatc
     9961 tcaccaagtg cgactcaaga tggattgact aaaggtcaag attacctctt tattggaact
    10021 ttccaagata gcttccaagg aaaaattatc tcaaactatg tttctgaaaa attaaatgct
    10081 aagaaagttg ttctttacac tgacaatgcc agtgactatg ctaaagggat tgccaaatct
    10141 ttccgcgagt catacaaggg tgaaatcgtt gcagatgaaa ctttcgtagc aggtgacaca
    10201 gacttccaag cagcccttac aaaaatgaaa gggaaagact ttgatgctat cgttgttcct
    10261 ggttactata atgaggctgg taagattgta aaccaagcgc gtggtatggg aattgacaaa
    10321 ccaatcgttg gtggtgatgg attcaacggt gaggagtttg tacaacaagc aactgctgaa
    10381 aaagcatcaa acatctactt tatctcaggc ttctcaacta ctgtagaagt ttcagctaaa
    10441 gctaaagcct tccttgacgc ttaccgtgct aagtacaatg aagagccttc aacatttgca
    10501 gccttggctt atgattcagt tcaccttgta gcaaacgcag caaaaggtgc taaaaattca
    10561 ggtgaaatca aggataacct tgctaaaaca aaagattttg aaggtgtaac tggtcaaaca
    10621 agcttcgatg cagaccacaa cacagtcaaa actgcttaca tgatgaccat gaacaatggt
    10681 aaagttgaag cagcagaagt tgtaaaacca taatagaaaa atgttgaaat agggaatgag
    10741 cctttgactc actccctgtt tcgatattta atactcttcg aaaatctctt caaactgcgt
    10801 caacgtcgcc ttggattata tatgtgactg acttcgtcag tcttatctac aacctcaaag
    10861 cagtgctttg agcaacctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat
    10921 aagaacctat caaaaagtga gggaaacccc tcggaattat aaatagaaag agtgaatctt
    10981 atgctccaac aactcgtaaa tggtttgatt ctaggtagtg tttacgcgct gttagcccta
    11041 ggatatacca tggtttacgg aattatcaag ctcatcaact tcgcccatgg tgatatttat
    11101 atgatgggag cctttatcgg ttatttcttg atcaattctt tccaaatgaa tttctttgta
    11161 gcgcttattg tagctatgct agcgacagct attcttggtg tcgtgattga gtttcttgct
    11221 taccgacctt tgcgccactc tactcgtatt gctgttttga ttacggctat tggggtttct
    11281 ttcctattgg agtatggaat ggtctatctg gttggtgcca atacccgtgc cttccctcaa
    11341 gcgattcaaa cagttcgata tgatttggga ccaattagct taacaaatgt gcagttaatg
    11401 attttggcta tttccttgat tttgatgatt ttgttacaag tcattgtcca aaagactaag
    11461 atggggaaag ccatgcgtgc agtatcagta gatagcgacg cggcgcaatt gatggggatc
    11521 aatgtaaacc gtacgattag ctttaccttc gctttgggtt ctgctcttgc gggtgcggct
    11581 ggtgttctga ttgctcttta ttataactct cttgagcctt tgatgggggt tactccaggt
    11641 cttaaatctt tcgttgccgc agtacttggt ggtatcggaa ttattcctgg tgcggctctt
    11701 ggtggctttg tgattggtct attggaaacc tttgcgactg cctttgggat gtcagatttc
    11761 cgtgatgcca ttgtttatgg aatcttgttg ttgatcttga ttgtccgccc agctggtatc
    11821 cttggtaaga atgtgaaaga gaaggtgtaa acgatgaagg aaaatttaaa agttaatatt
    11881 ctatggttac tccttttgtt agctggctat agcttgatta gtgtactggt ttcagtcgga
    11941 gtacttaatc tattctatgt acagatttta cagcaaattg gaattaatat tattttggct
    12001 gttggtctca acttaatcgt tggtttttca ggacaatttt cacttggtca tgctggtttc
    12061 atggcgattg gtgcctatgc agcagctatt attggttcta aattaccaac ctacggtgcc
    12121 ttctttggag ctatgcttgt aggggctttg ctttcaggag cagttgcctt acttgtcggc
    12181 attccaacct tgcgcttgaa gggggactat cttgcggtag caactctggg tgtttctgaa
    12241 attatccgta tctttatcat caatggtgga agccttacaa atggtgcggc aggtatctta
    12301 gggattccta actttacaac ttggcaaatg gtttacttct ttgtcgtgat tacaaccatt
    12361 gcaaccttga acttcttgcg tagcccaatt ggtcgttcaa ccctctctgt tcgtgaagat
    12421 gaaatcgctg ctgagtcagt tggggttaat acgactaaaa ttaaaatcat cgcttttgtc
    12481 tttggtgcca ttactgcaag tattgctggg tcacttcagg caggatttat cgggtctgtt
    12541 gtaccgaaag attacacctt catcaactca atcaacgttt tgattattgt tgtatttggt
    12601 ggactcggtt ccattacagg tgcgattgtt tcggctattg ttctgggaat tttgaatatg
    12661 cttctccaag atgttgctag tgtgcgtatg attatttacg ctttggcctt ggtattggta
    12721 atgattttca gaccaggtgg actccttgga acatgggaac tgagcctatc acgtttcttt
    12781 aaaaaatcta agaaggagga acaaaactaa tggcattact tgaagtaaaa cagttaacca
    12841 aacattttgg tggtctaaca gctgttggag atgtgactct tgaattgaac gaaggggaac
    12901 tggttggatt aatcggtcca aacggagctg ggaaaaccac ccttttcaac cttttgaccg
    12961 gtgtttatga accaagcgag ggaacagtaa ccctagatgg tcaccttttg aatgggaaat
    13021 caccttataa gattgcctct ttgggacttg gacgtacttt ccaaaatatc cgtctcttta
    13081 aagatttaac agttttagac aatgttttga ttgcttttgg aaaccatcac aaacaacatg
    13141 tttttactag tttcttacgc ttaccagctt tttacaagag tgaaaaagaa ttaaaggcta
    13201 aagctttgga attgttgaaa atctttgatt tagatggtga tgcagagact cttgctaaaa
    13261 atctttccta cggacaacaa cgtcgtttgg aaattgttcg tgcccttgct acggaaccta
    13321 aaattctctt cttagatgaa ccagcagcag gtatgaaccc acaggaaaca gccgaattga
    13381 ctgagttaat tcgtcgtatc aaagatgaat ttaagattac aatcatgttg attgaacacg
    13441 atatgaatct ggtcatggaa gtaacagaac gtatctacgt acttgaatat ggccgtttaa
    13501 ttgctcaagg aactccagac gaaattaaga ccaataaacg cgttatcgaa gcttatctag
    13561 gaggtgaagc ctaatgtcta tgttaaaagt tgaaaatctt tctgtgcatt acggtatgat
    13621 ccaagcagtc cgtgatgtaa gctttgaagt taatgaagga gaagttgttt cccttatcgg
    13681 tgccaacggt gcaggtaaga caactattct tcgcaccttg tcaggtttgg ttcgaccaag
    13741 ttcaggaaag attgaatttt taggtcaaga aatccaaaaa atgccagctc agaaaattgt
    13801 ggcaggtggt ctttcacaag ttccagaagg acgccacgtc tttcctggct tgactgttat
    13861 ggaaaatctt gaaatgggag ctttcttaaa gaaaaatcgt gaagaaaatc aagctaactt
    13921 gaagaaggtt ttctcacgct ttcctcgtct tgaagaacgt aagaaccaag atgcagctac
    13981 tctttcagga ggggaacaac aaatgcttgc catgggacgc gctcttatgt caacaccaaa
    14041 acttcttctt ttagatgaac catcaatggg acttgcccca atcttcatcc aagagatttt
    14101 tgatatcatt caagatattc agaagcaagg aacaaccgtc ctcttgattg aacaaaatgc
    14161 caataaagca cttgcaatct ctgaccgagg atatgtactg gaaacagggc aaatcgtcct
    14221 atcaggaaca ggaaaagaac tcgcctcatc agaagaagtc agaaaagcat atctaggtgg
    14281 ctaaaacaat ccagtggatt gttttagtcg gcagatgaag attacgaagt aatcatcatt
    14341 atagtctggg agactagttt aggttgcaga taaagattgc gaagcaatca tcaaatccag
    14401 tggattgttt tagtcggcag atggagatta cgaagtaatc atcaatatag tccgggggac
    14461 ctttttagtc ggtagattga gattgcaaac aaatctgcat ctacattgaa agcttaattt
    14521 ctaataattg aaaaaatcga atgaaaaatt tcttaccttc attcacagag ctcgatttca
    14581 gagctctttt tgctagctta ttcatacttt tctgaatttc gaaaaagaaa tgtaagcgtt
    14641 tgatagattt acaaaaagat tgtataatag ggataagaat agaaaaggag aagtctcatg
    14701 gcagttaaag attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct
    14761 catgcagcag atttgatgag agagcaaggt ttgcaccgtc tgcctgttat cgaaaatgat
    14821 caattagttg gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca
    14881 agtctttcta tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg
    14941 attcgcgatg ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg
    15001 ttgaaaaata agattagtat tctccctgtc gtagataacc atcaagtata cggagttatt
    15061 actgaccgtg acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt
    15121 cgtgtacgct ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt
    15181 gtagaagaaa atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtg
    15241 attatcgaag tgcaaatcga tggatcaatt gatttaccag ccttgaaaga aaaatttgaa
    15301 gcaaatggta ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg
    15361 aagcccaaag gcttcttttt tcatgaaaag gggattagag aaaaagatgg aaagaaatga
    15421 taaaatatgc tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc
    15481 gtcagaaaat tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc
    15541 gaagggctag aggaggagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg
    15601 aacgataata ttgcagccca aaaaacgtcg caagaattaa atgaattaaa aaacacttac
    15661 aataccttcc ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg
    15721 gctgaagacg agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata
    15781 atgaccagct acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc
    15841 ttggaaatcc atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt
    15901 cgtatgtata ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa
    15961 gcaggtgatg aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat
    16021 ggtctcctca agtcagaaat gggtgttcac cgcttagtgc gaatctcacc atttgactct
    16081 gccaaacgtc gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact
    16141 attgaagtgg aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc
    16201 ggtggacaaa acgtcaataa ggtttcaaca ggtgtacgtt taacccacat tccaactgga
    16261 attgttgtcc aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag
    16321 atgttgcagg ctaagctcta tcaaatggag caagagaaga aggctgcaga ggtagattct
    16381 ctcaaaggtg agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg
    16441 ccttatacta tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt
    16501 atggatgggg acttagatgg ttttatcgat gcttatctca agtggcgaat tagctaagat
    16561 agaaaggaac tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg
    16621 gaacgactgc tctacgcggt gtttcggtta gcgttcaacc gggggaattt gcttacatcg
    16681 taggaccttc aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa
    16741 tcgataaagg aagcctatca gttgctggtt ttaatctggt taagatcaaa aagaaagata
    16801 tcccgcttct acgtcgtagt gttggggttg tcttccagga ttataaattg ttaccaaaga
    16861 aaactgtcta tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata
    16921 tcaaaagacg agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt
    16981 tcccaaatga actctcaggt ggggagcaac agcggattgc gattgcgcgt gcaattgtaa
    17041 ataatcccaa agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat
    17101 gggaaattat gaatcttttg gaacggatta acctacaagg aacaactatt ttgatggcga
    17161 ctcataatag ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc
    17221 gtgtcgttcg tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc
    17281 catttatttg aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc
    17341 agttcagtca tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca
    17401 gcgaaactag ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat
    17461 gtggaagata atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac
    17521 cacaaggtat atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt
    17581 aaagaagaac aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa
    17641 ggagatgcca atcctctcta tgatgcctat attgtagagg caaacactcc aaatgatgta
    17701 aaaactatag ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt
    17761 gccaatacag aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt
    17821 gctgctttgt taatttttat cgcagttttc ttgatttcaa ataccattcg tattaccatt
    17881 atttcccgca gtcgcgaaat tcaaatcatg cgcttggtcg gagctaaaaa cagttatatc
    17941 cgtggaccgt tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct
    18001 gttttggtct ttattgttta tcaaattgtt taccaatctg tcaacaaatc gttggtaggg
    18061 caaaatctat ccatgattag tccagattta tttagtccgt tgatgattgc cctactattt
    18121 gtgattgggg ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgaag
    18181 gtttaggtaa aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga
    18241 gtttttgaaa agagatgcgc agaagaaaag agcttccaaa gaagtccccc agagaagact
    18301 tcttgatggc taatttgaac ttgaaatttg gcgcactaga aattttttag aaaaaagtgt
    18361 aattttttta
//
LOCUS       DAVKFH010000032        17472 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKFH010000032 DAVKFH010000000
VERSION     DAVKFH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.032"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(518..1654)
                     /locus_tag="VW006_001299"
     CDS             complement(518..1654)
                     /locus_tag="VW006_001299"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU9380152.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNDFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPTQSHEDSLFVMVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(1716..2153)
                     /locus_tag="VW006_001300"
     CDS             complement(1716..2153)
                     /locus_tag="VW006_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9380153.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(2155..4110)
                     /locus_tag="VW006_001301"
     CDS             complement(2155..4110)
                     /locus_tag="VW006_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9380154.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEIMKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(4134..5903)
                     /locus_tag="VW006_001302"
     CDS             complement(4134..5903)
                     /locus_tag="VW006_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU9380155.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            6112..7452
                     /locus_tag="VW006_001303"
                     /pseudo
     CDS             6112..7452
                     /locus_tag="VW006_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7580..8602)
                     /gene="cbpF"
                     /locus_tag="VW006_001304"
     CDS             complement(7580..8602)
                     /gene="cbpF"
                     /locus_tag="VW006_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEU9380156.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRQEIAFRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWV
                     YDDGDWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQHLGNKWYYLRSSGAMATGWYQDGSTWYYLDAENGDMKTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(8621..9442)
                     /gene="cbpG"
                     /locus_tag="VW006_001305"
     CDS             complement(8621..9442)
                     /gene="cbpG"
                     /locus_tag="VW006_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU9380157.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHSYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(9523..9762)
                     /locus_tag="VW006_001306"
     CDS             complement(9523..9762)
                     /locus_tag="VW006_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380158.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(9912..10178)
                     /locus_tag="VW006_001307"
                     /pseudo
     CDS             complement(9912..10178)
                     /locus_tag="VW006_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10203..10514)
                     /locus_tag="VW006_001308"
     CDS             complement(10203..10514)
                     /locus_tag="VW006_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9380159.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(10439..10670)
                     /locus_tag="VW006_001309"
                     /pseudo
     CDS             complement(10439..10670)
                     /locus_tag="VW006_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10671..11303)
                     /locus_tag="VW006_001310"
     CDS             complement(10671..11303)
                     /locus_tag="VW006_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380160.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(11317..12312)
                     /locus_tag="VW006_001311"
     CDS             complement(11317..12312)
                     /locus_tag="VW006_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9380161.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(12309..13007)
                     /locus_tag="VW006_001312"
     CDS             complement(12309..13007)
                     /locus_tag="VW006_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9380162.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(13084..14094)
                     /locus_tag="VW006_001313"
     CDS             complement(13084..14094)
                     /locus_tag="VW006_001313"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9380163.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(14078..15085)
                     /locus_tag="VW006_001314"
     CDS             complement(14078..15085)
                     /locus_tag="VW006_001314"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9380164.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDILIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSFEEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(15072..16025)
                     /gene="mvaD"
                     /locus_tag="VW006_001315"
     CDS             complement(15072..16025)
                     /gene="mvaD"
                     /locus_tag="VW006_001315"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9380165.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEYLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(16007..16885)
                     /gene="mvk"
                     /locus_tag="VW006_001316"
     CDS             complement(16007..16885)
                     /gene="mvk"
                     /locus_tag="VW006_001316"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9380166.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITETCVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(17007..17186)
                     /locus_tag="VW006_001317"
     CDS             complement(17007..17186)
                     /locus_tag="VW006_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9380167.1"
                     /translation="MLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNT
                     TVDGYFVNYNGEWVQ"
BASE COUNT         5377 a         3832 c         3055 g         5208 t
ORIGIN      
        1 tcttgtttca ttttactata tagatcacat tcgaaagaat tcgttccaaa attgcttggt
       61 tcgttcttct cttggacgac taaagaattg atgggcatta tttttttcaa caattttccc
      121 actgtccata aaaataacat gatgggctac atctctagca aaacctatct cataaagctg
      181 atcaaaaact caatctgagg agaaaatcca ataagacttt ccataataaa ggacatccta
      241 tcaagatttt tgaacacccg ataggctgcc tttttccgat tttgaagact ttttgtccag
      301 cttcttttac cttataaata acaacaaaaa tcaaagacaa gctagaaaga ttaccgcaga
      361 taactcaaac agtgttttga ggttgcagat aagactgacg gagtcagctc aaagcactgc
      421 tttgaggttg tagatagaac tgacgaagtc agctcaaaat actgttttga ggttgcagat
      481 ggatgctaac ctgatttgaa gagattttcg aaaaagatta aatatactct acaatttgct
      541 caatcaattc ttggtcgtct aaacctgtaa cttgtataac aacatctttg actgattgtt
      601 tagccaacaa ttcacctaat cgaatacttt cttcatcatt tacatcgcga tagtcaaagg
      661 catagccaac tgttttaagt aggtttttat atgacaaact gagttctttc aattcacgta
      721 tcggccggat gaatcgttca ttatagccta attttcggat tggagtacga gctacgcgac
      781 taacctcgtc cactatgaaa gggttttcga atcgttctat aatgactttg tgataattct
      841 ccaattcttt tttatcaaag ttccatttgg caatcaagag actccgaatt tcagctaata
      901 cagattcaat ccgagatttg atattaggat tttgaagagc ttccaaaatt gtcttggcac
      961 cataatgcgc accaatgtaa gctgaagttg catgtccaga attgactgaa aaaagttttc
     1021 gctcaataaa gggttctaaa tcttcttcat aatgcacatc ttctagacgt aaatctggat
     1081 ttttaagacg cttggtttcc acgacccatt cattaaaggg ctcgaccata acaaaaaggg
     1141 aatcttcgtg actttgtgtt ggaacaatcc tgtctactgc agcatttgga aaacctatgt
     1201 agttatcagc aaatgtcaaa ccttccggac ttaaatattt cttgacttct tgataaagaa
     1261 attgagaccc gccaatcata ttttcacagg ccataacatc caatgcctgt gtatttcctg
     1321 caactcggcg agcttcgatt cctttggcta gaagttcagc gataaaaggg agtatattag
     1381 gtccgattgc agtagtaata atatccgtct tttgaatcgc ttcaatgact tgctcaggat
     1441 gttctttgct attaattcca gccacattag ttacttctat acgagactgt cctttctgtg
     1501 caatttcaat ttcatacttg cccttttcat ttagagcatg aattatctga ttattgacat
     1561 ccacaaaatc aatatggaaa tcatttttaa atagaatttc acctataaaa ccacgaccga
     1621 tattaccggc accaaaatga acagaatgtt tcattcaatt tctccttttc ctatttgagg
     1681 gacatatata ctagatgaca aatgacgaaa ttcaattatt caacagcatc aaataagcgc
     1741 aatacttcct cttttgactg agcatctgct agtttaagaa cattatctac atctgcacag
     1801 aagatagaaa ttttctgaat aatttctaag tgttcattac caataccagc aataccaaaa
     1861 agaaccgttg ccacttgagg gttagataca ttcccaaaat caacccctct agggacttga
     1921 acgactgtaa taccagactt gaggacatca ttttttgctg catctgttcc atgcggtatg
     1981 gcgataaagt tacccatgta aacagatagc tctttatctc gctcaatcat ggcttcaata
     2041 tagtcttcat taacatatcc accctcataa agaacttgcc cacaataaca aatagcttcc
     2101 tccttgttag aaaattgttt attaagctta atcaaatgtt tttcaagttt catattagtt
     2161 ctccaatttc ttaatttttt cgttaaaaat agtatttagc atctgataaa taatggattg
     2221 atttcccgtc ttgtaaatct ctgtataaag atgattttca ataatcgact gactgatagc
     2281 tgtcattaaa tctctcgctt cctcgctagc ttctttagac attagcatta gcagacacct
     2341 tttgacctct tcttcctctc gtttcattga caatgcggag ataggtagtt tgagttcaaa
     2401 catagtaaaa ctatttgttg ttactttgct agactgacta tgtaaaagca ccagaccagt
     2461 attaggaata gccataggac tattctggaa gtgagataac aattttcttg ttaggtaatc
     2521 tctgtcactc acattcttct gatattggat gatttgttct accgtgtgct caaatgattg
     2581 attattctcc aattgaacca actcgaattg actcaaaagc tgactactag atattaaata
     2641 atcttgcaag tcataacttt tctctggagc gatagcatca cgcgcgacaa ttgtacgatg
     2701 tgcctgtaca tactgaagtc gttcctgtaa ttcaagcaat tctttgacgg ttggaaaact
     2761 agaaattaca tcgatttcct gattagtcag cggcttggta gataaaatat aatcatactg
     2821 actgagatca atactgtggt aatctactag agactgaata tctatcaact ctacaaaagg
     2881 agcaacactc ttaattttac tgactaataa atctctagtg acccgacgtt catccgttaa
     2941 aagcaaaaca cggactggat aaatctctgg actacggcct aagctagaga taaaatgtag
     3001 ggcaatcatg ccatactcat actctgtatg aagatatttc ggaaaaatat cattcactaa
     3061 aagactgatg actgtataaa gaaatttatt cctttcaact aaaatctgga cagattctgg
     3121 caaattttca ccctgaaaaa ggatagggac gcctaaactg agccgaatat gatggaaaag
     3181 aaaattgaat aaaaccttgt ccttaaaaaa gtcaatcttg gtatacatgg aaaccgtatc
     3241 aatcagattt gaaatattgt agaaaaattc accatcaaat ttctccgtaa agagcggatt
     3301 gtcctgacgt ttaatgatta attcatccaa gatgctcgca aaatagataa tttcctgaat
     3361 actataaaat tgtgcagtct gcttagagta agtttgaaaa attttttgag aaatttccaa
     3421 agcctgctta ctagtattag gtgaattttc aatgttttgc tcctgaccta taagagataa
     3481 caagatcgca aaaaacatct tcatcctagc atccatatct ggaaaacctg acagttgctt
     3541 attaacaatc tgactggcca gcctagttct atctgcttct aaaatatcaa agctcccaaa
     3601 attaccggtt gaaaaatctg ctactgagat acagtttgtc agtaaaatag ccaaaaatct
     3661 tctcttacca actgaatcac cagaaatccg ataacctttt tgtcgttcaa ttttcagatc
     3721 aaagtctaaa agacgcttat caatatctga aatatcctga ataatagtta cattactgac
     3781 tttcgtgcac tcttgcaatg cttcattggt gacaaaaccg ctctcagtca gaaggcgata
     3841 ggtaatcaac tcttgtcttt cttggggact atactcaact aacacttcaa gttctgtcgg
     3901 caaatcatcc aactctccag tcaaaatata gtatttccca tgcttcatta tttcgattcc
     3961 atattgctcc atgctatctg tcaaatctga taaagttcga taaattgttc tagatgaaac
     4021 ctgtaagatt tcagtcatat cttgcattga aagcttccct acatgtagga aagccttcaa
     4081 taattgttct tctcgtttgg ttaatagcat tcgtaattta gaaacctcta gttctatttg
     4141 tacattctag cagccatttt gtcatattct ggtgttgtta ctaaactatc cacaataaga
     4201 aattgagaat tcggtgctgc ttgctgcact tctgcctgta aagaaatagt tgttacaatc
     4261 attatgtttt tagaattaaa ttcacctaat tctgaaattt tggcagtaga aactggaata
     4321 cgaatattct tgtttctaaa gatagcccga atcgtttcac agcccatagt tgcagttccc
     4381 tgtgctttac cataagcaac tactacagca tcaatatggt taaggtcaat ttcctgacta
     4441 tctactggtg ctgaagttgg tatatctcct tcaatttctg ctattggaga agctcctgtt
     4501 aatgaagcta caatttcatc ataacgaggg gacgctaaga aattatcaac agaaacatga
     4561 atagcacttg gactcttgtc tttagctctt ggtgtcagtt cttcctgagt aacaattaat
     4621 gtttttggtg tatcaagcaa atttgagatt gcctgattag atactggaat ctctagacct
     4681 gcttttttaa ccttatctcg aagaatacta gctcccatag cagagcttcc cataccagca
     4741 tcgcaggcga aaatgatttt ttccactgag tctgtcgaaa caactgcatc aacagaagtt
     4801 gatactaact gacctttaga ctgagcctta gctgcttggg tagccgcctg agcagcttcg
     4861 agcgaatcct cagttgactt gtctgcatga agaataaggg ctgctacaag gaaagaaaca
     4921 actgctgcta ctaaaacacc tagaagaaca tttagatggg gccaaacacc ttttggcgcc
     4981 gtagccataa tcgcaataat agaacctggt gaagctggag atttaagacc agcgtctaag
     5041 agttgaaaag taaaagttcc agagatacct cctgccatag cagctaaaaa tagagtaggc
     5101 ttcatcataa cataaggaaa gtaaatttca tgaatccctc cgaagaaatg aataaccatt
     5161 gccccccaag aagaagattt agcagaacct ttaccgaata cagcataagc taatagaatt
     5221 cccaggcctg gtccaggatt agcttccaat aggaagagaa ttgacttacc agcttgagct
     5281 acctgttcta ctcccagagg agtaaaaatg ccatgattga gggcattatt gaggaaaagg
     5341 actttagccg gttcgatgat aatattagcc ataggaagga ggcgagcatt gacaatagcc
     5401 tcaacaccat tcccaacagc tccagtaaga gtcgatacga ctggaccgat tgcgtagaaa
     5461 gccaaaagca ataatgcaaa accaacgaga ccagctgaga agttattaac taacatttca
     5521 aatccgggac gaattttttc ttggaacttc tcatcaaatt tcttgatagt ccatcctccc
     5581 agtgggccca ttaccatagc tccgataaac ataggaacac tagaacctgt gattgcacca
     5641 acagtagcaa tagctcctac aacggcacca cgttggccat ggatcatata tccacctgtg
     5701 taaccaatca ggattggcaa taaatacgtt aacataggac caacaacagt agctaactgt
     5761 tcatttggca gatagccatc agcgataaag agggcagtca atactcccca agcaataaat
     5821 gctccaatat tgggcataac catatttgaa agcgatgtcc ctagtttttg aaccctgact
     5881 ttcaatgata ctttttcttc cattagatca tcctcctttt cttaaaacta gtatatacaa
     5941 agttaaaaac ctttaaaact aaagttttgt cacagatttt gtgtcaaaac attacttttg
     6001 acgaggctgg gcaaaaaggc ttaaaaatta taaaacgcat catatcaggt gtacatgaac
     6061 tacaccccaa aagttagaca gaaaaaatct aacttttggg gtgtttttat tatgaaacta
     6121 acttatgatg ataaagttca gatctatgaa cttagaaaac aaggatatag cttagagaag
     6181 ctttcaaata aatttgggat aagcaattct aatattaggt acatgattaa attgattgat
     6241 cgttacggaa tagagttcgt caaaaaagga aaaaatcgtt actattctcc tgatttaaaa
     6301 caagaaatga gtaataaagt ctgacatgaa ggctggacta aagatagagt ttctcttgaa
     6361 tacggtctcc caagtcgtac gatacttctt aactggctag cacaatacag gaaaaacggg
     6421 tatactattg ttgagaaaac aagagggaga gtacctaaaa tgggacgtaa gccaaaaacg
     6481 agacctgaag agaggacaga attagaacgt cttcaagcag aaaatgagta cctgagagcg
     6541 gagaatgcca tcctaaaaaa gttaagagaa ctccgattga aggaggagaa agaagaaaga
     6601 cagaaattgt tcaagaatta atgactgaat tttcgttagc tcttcttcta aaagtcatta
     6661 aactagctcg ttcgacctac tactatcact tgaaacagct agataaacca gataaggact
     6721 aagagcttaa agctgaaatt caatccattt ttatcgaaca caagggaaat tatacttatc
     6781 gccgggttca tttagaacta agaaatcgtg gttatctggt aaatcataaa agaattcaag
     6841 gcttgatgaa agtactcaat ttacaagcta aaacgcgaca gaaacgaaaa tattcttctc
     6901 ataaaagacg ttggcaagaa ggcagagaat ctcattcaac gccaatttga aggctctaaa
     6961 acaatggaaa agtgctacac agatgtgaca gaatttgcca ttccagcaag tactcaaaag
     7021 ctttacttat caccagtttt agatggcttt aatagcgaaa ttatcgccta taatctttca
     7081 acttcaccca acttagaaca agtacaaaca atgttggaac aggcattcac agagaagcac
     7141 tacaagaata cgattctcca tagtgaccaa ggctggcaat accaacacga ttcttatcat
     7201 cggttcctag agagtaaggg aattcaagca tccatgtcac gtaagggaaa cagcccagac
     7261 aatagtatga tggagtcctt ctttgggatt ctgaaatcgg aaatgtttta cggttatgag
     7321 aagtcgtttc agtcgcttaa gcaattggaa caagccatta tagactatat tgattactac
     7381 aacaataaac gaattaaggt aaaactaaaa ggacttagcc ctgtgcaata cagaactaaa
     7441 tccttcggat aaattaattg tctaactgtt tgagggcagt acagaaaaac cttgatacta
     7501 tgcgtttttt gattgtgaag acttttatcc acctattata caaagttaag tatccatcac
     7561 agtttacaat tagccttcat tacttaaccc attcaccatt atagtttaag tagtaaccac
     7621 ctactgtggt attaacagct aaagcacctg aatcataggc atagtaccag ttaccattga
     7681 cttggaacca gcctgttttc atatctccat tacttgcatt tagatagtac caagtcgaac
     7741 cttcctgata ccaaccagtt gccatagctc ctgatgaacg gagatagtac catttgttcc
     7801 caaggttttg ccaaccagtt ttcatatcgc cattttcagc atctaaatag taccaagttg
     7861 aaccatcttg ataccagcca gttgccatag ctcctgatga acggagatag taccatttat
     7921 taccaagatg ttgccaacct gtttgcatga tgccagttgc tggatctaga tagtaccatg
     7981 gagctgcttt tagcttctct tcatcatagg taagagggta atccttaacc caaccacgtg
     8041 ctagctctcc aaccgtcaat ctgttgatgc gagaatcaaa gccaccatcc ttctgcaaat
     8101 aataccagtc cccatcatca taaacccaac cagtttttaa agtatgataa ctacgctgat
     8161 cttcaaaata atagactcgt ttctctgctg ggctatcata ttcttcccca tgatgtttgt
     8221 tggtatttgt agcagttttt gcttctaaca cttgcttgcc aacaaattct tgtagtaccc
     8281 catcttgacc aaagtaaaac caatctggtc taaaagcaat ctcttgtctt ggagaaggac
     8341 caatcgtaac ccccttgtgt ggagcaggta tatattgcca gccgacaacc atctctccgg
     8401 ataaaggatc aaaataatag tatttcccat ctatcactct ccagtaggtt tcttttagat
     8461 cccccttttt gtagtaggtt ctaccatttt cttggacaaa ctgccatcct tcagaatcat
     8521 ctgcaaatac tgtactggtc cctagcaaac cgaagaaaaa tgtggctagt gcgacttgca
     8581 tcattttttt caaaagcttc attttatata tcctccaaca ttaaatccac tcaccagatg
     8641 aagcgaagtt ataaacttta ccatcgatag tttggctacc tgtaaccatt gctccatttg
     8701 aattgagata ataccatttt ccatttactt tttgccaatc tgtaagcatc ttaccggaac
     8761 tgtctaaata ataccaagta tcatttatct cctgccaacc cgtttgttta tcatgttgtc
     8821 tataatggta ccaactacca tttattttct tccatccttc aagagagtaa cctttaagaa
     8881 ccgaataaat aaatggcaaa tttcgttcat ttaatttaac tgcactgtta atttgattag
     8941 ctccatctcc taaagtatgc actcctacta cacggtgact agcatcataa actgtagatc
     9001 cactagaccc ctctaaagta tcaacttggt aatccaagaa catgccatca tcacttaaaa
     9061 cttgtttttt atctgtatac atttgatgaa ctttaaaatt atatgatgga tagcctgtga
     9121 tagtcacagt tattcctgtc aaattttttt gactagtagg aagacccaaa gtccctaatt
     9181 ttgcaccaat gggctcttct agaattaata aagccaagtc atattccctt gcatccttag
     9241 aatttaaatt tctaaattcc ttcaaataac gaacttcctt tactttgatc tttccaaatg
     9301 gttcttgact tggactaaca gccggaagaa cataaatatc atccgcttct ttcccatagt
     9361 catgtctgta gtagctgtga gccgctgtaa ttaacacatt atctttaata aaattcgcac
     9421 ttcccctccc ttctacattc attccatcgg ctactccata atacttagat aaaaccatag
     9481 ctgaagtcga atacggatac tgtaaagtat tatcaatttt aatcaaatca tcattaccga
     9541 taatacttct gattgctttt ggtagtatga accatatgtt ggtgaaatct cagataatga
     9601 agaatcatta gatcctggac ctttttctag tgtctcactt acctcatatt cttcacccgt
     9661 actagaaata acactcaaag cagatactgt cgataactgg ctagccaata aagtactcgc
     9721 aataattgaa atacccaatt ttttataaac agttttcttc attattgtat cctcctaatg
     9781 taattatagc gtactattct aaatttctta atctactata gaatcaagaa atctaccacc
     9841 ttctttaaat accctccatt atcacataaa caggtaaact tttcaattaa tgactgcgct
     9901 tttcaatcac gctagaggta cttacttgct tctttgatac taagttcagc cattctttcc
     9961 ttgtttttct caataaagca tgttacccaa gtgggattcg ttttggagta gtctcgcaga
    10021 gtccagccaa tggctttatt gataaaaaat tctgtttggt tcaagttatg aaggagaatc
    10081 ttttccatta attgagtatt ggtcttctct tttcttaaca actggttgtc aatagcgaca
    10141 cgtctcagcc agatattatc tgataggctc cattttatac tcaatgaaaa tcaaagagca
    10201 aactaggaag ctagccgcag attgctcaaa acactgtttt gaggttgcag atagagctga
    10261 cgtggtttga agagattttc gaagaatatt aagattattt cttctagttc aggatgttta
    10321 tacaccaaac tccctactac tcgatctagg atatctaccg tgtcccacca ggattttgtc
    10381 acgactaact gctctagctt aggcaaatcg gtttccttta gataagactg cattgctttc
    10441 aaatagttag cagccacata ttggtatttt ctaggctcct tttcccagca agtgtctgca
    10501 aaatcccaat cgataatctt tgtttttttc gcttctggaa aatattttta tagagtttat
    10561 ttctttcaac ctagaaaaga aaattgatgg cacatatagg cttccatggg ccttgctttt
    10621 ttagagtctt ttgctgcttc tagctcctca agtaaatctg ctaaactcat ctaaaactcc
    10681 tcatgcccca ccaaatggtg ctgaaaggca tagacagccg cctgggtacg atcgctaact
    10741 tcaagtttgg caagaatgtt ggacacgtgg gtcttgaccg tcttgagaga gataaagagg
    10801 tcatctgcga tgcgctgatt ttcgtagccc ttggcgatga gttggagaac atctcgctca
    10861 cgcgcagtca attcttcatg aagttccata tgattgcggt ggtattcaac cttcttgcta
    10921 acctcttgct caatggccag ctcgccagca gctaccttac tgacggcatg aagcaattca
    10981 tctgcactag aagtcttgag catatagcct ttggcaccag catctaagac tggcatgatt
    11041 ttttcattgt ccaaataaga ggtcacaatc aaaatcttgg cttcaggcca ttctttaagg
    11101 attgctaagg tcgcgtcaat cccattcatc tcaggcatga caatatccat gacaatgaca
    11161 tctggacgca gttccaaggc caagtcaatc ccttgagacc cgttggacgc ctcacccaca
    11221 acttctacat cgtcttggag gtcaaagtag cttttcaagc ccaatcggac catttcatgg
    11281 tcatctacta gtaaaatttt catctttact cctttatcat tccttatcta acaggggaat
    11341 acggatatca accgccagcc cttgcttggg agctgtcaag agttgaactg ttccagccat
    11401 atcttcaacc cgctccttga tatttcgcag tccataactc aagtcgtcta agctccctaa
    11461 ctggaaacca atcccattgt ccaccacctt cagttgcaat tcaacatctg tctgatagag
    11521 gtagacatct aggcaagatg cctgggcatg gcggagggta ttgctaatca actcttgcag
    11581 gatacggaag atatgctcct cgattttctt aggcaatttc gtcatattct gcttgagact
    11641 aaccctaaga tcactcttgt cctcaagctc ttttaagaga atctgaatcc cttctatcaa
    11701 gctcttctgc tccagttcaa ctggtcgcaa atgcaagagc aaaacccgca aatccttctg
    11761 ggctgtttct aaaatagctg tgacactctg caactggatc tgcatctttt ctctatccaa
    11821 tttcaaagcc tgctgactga tacccgataa aatcatgtgg gccgcaaaca actcctgact
    11881 gactgtatcg tgcaaatccc gagcaattcg cttccgttcc ttctcgatga tttcctcttc
    11941 ctgagcaagg ctctgatttt cagctttttg aagagcttct gtcaaaaggt taagtttacc
    12001 tgataaggac ttgaaactgg catccaaatc tggatctgca acctgaacca cttcttgccc
    12061 tgccaataaa cgcttgagat tagcctgcat ttttcttaga gaaagctctt cgatccctcg
    12121 ccaaaacagg gctaagagac aggttatgga catgctgaaa accaacaata aaaagacaaa
    12181 tttttctgtt ttttcgacat cgtgcaaaaa gatagaccag tcaaaatcaa gtatttccag
    12241 caagctgtgg gagaaaaaaa agacaaacag gaaggaggtg agagcaataa tgacataggc
    12301 ttgttttttc atcctctaac cacctccaca tcaccaatca tagtggtcaa gaaaatcttg
    12361 acactcttgt tactcttgag atagtctttt gtttcttgat gatagtgttc attgcggagg
    12421 gctcgcttgg gctggttgaa aaaagtcaaa tccccataga gacagttaac gctgagactg
    12481 acttccacat ctacaggtac gatgattttg gtcgttccta ccatctttct gaggataatg
    12541 acattgtcat gattggttaa gatgaccctc tccagatgaa tagtgtcctt gcccatgaag
    12601 cgaaagagat tgatatcatc gaattggcaa gtctggtagc ttgaaaaatg atgaagattt
    12661 ccaaaccaac gatttttctc cttcttaacc gtcacgacct cttcaaaaac caaattggtc
    12721 tgctcttttt cctggttcat catcgggtaa agaagaaaga ggctatagat aaccgcaaca
    12781 aaaatagcta gaatcacaaa aggattgagc ataacgatga aaaagaagag aatggttgcc
    12841 gctactaaaa gaagattatt tccctcttta ccagtgtagt agcgaatcaa aagcaaaaag
    12901 aggaatagta tcagcagaaa acgcgaaaaa tgctctgata ccatcaaaat cagagctcct
    12961 gtcagaagac aggcttcgat aaataaaaag attttaaatt ttctcatagg ttcatcctct
    13021 cccttctatt ttatcacaat tcaaaaaagt cacctcagtc tgaggatgga aaaaaggcgc
    13081 tggttacgcc tttttcatct gatcctttgc ttcttttaat tttccataaa gaagatagtc
    13141 tactttttgt agatctgcta tggtggcaca gttaagggaa cacataatca agcgtaggtc
    13201 tgctttccag ccttggacaa tgccaatcac ttcttcaact gtgtaggttt caaccaattc
    13261 cagaacggtt cgtgacaatc ccacagactt agcaccaaaa accaagcact taatcatatc
    13321 cagcggattc cgaacccctc cactaaccaa gagttcgacc ttatctttcc attcttgggc
    13381 attgagaagg gcctgcatgg tagactgacc ccattgattg aggtaatcac gctggccact
    13441 acgacggttt tcgatatagg caaagctggt gccaccacga cccgataggt ccactgtacg
    13501 aacaccgaat tcataggctc tttcgattgt cttggcatcc attccaaagc ccacttcctt
    13561 gaggacaata ggaacgggaa tttgctcgct ataatctgct agatgcgatt gccagcttct
    13621 aaacttcctt tctccctcgg gcatgagtaa ttcctgcatg acattgacat gcacttgcaa
    13681 tagaacagga ttcatctctt ctacagtctg aagtcctaac tcgacaggct tgtccaatcc
    13741 aatattggtt ccaaggagga gattgggatg actagacttg acagaaaaag aatcatccgt
    13801 tggatttttg agggctgcgc tataagaacc cgttacaaat aaaataccac aggattccgc
    13861 cacctgagcc agcttttgat tgatttctct tcccttatta cttccaccag tcatggcatt
    13921 gatataaaaa ggaaagtccc actttcgacc agcaaactct gtcgaaagat cgatttcatc
    13981 cagattgtaa agaggcaagg aagaatgaat cagctccacc tcatcaaagc tattatagga
    14041 acttttctgc tcaagggcat agaggatatg ctcgtcctta cgatttgtcg tcatgtccta
    14101 tcctttcttg atataagagc tcaatcccca gatcggccca acgatttttt aaggttttgg
    14161 ttgattgcgc atcaaaactc agggcgatgc cacagtcacc accaccagca ccactactct
    14221 tggcaacggc ctgcaaatct tgactggctt ctttcaactg tctaagcaaa ggcgtgtaaa
    14281 tatctgtact caagccttct aaaagcttgc tggctacttc tacttgctcg ataatctttt
    14341 ctgatttccc ctgttccaag gcttcctcaa aagaaaccac cgtttctttt gaggaagtta
    14401 aaaaattttg attgatattt tgcttgattt gctggaccat gtgactcgat acagccactt
    14461 ccttggtcca tcccactaag aaatcacatt ctaaagttgg tttcacttgt gaaattgaaa
    14521 agccccaatc acgctccaga actgtcgcca agttttcttc ttctaaccaa gcagccacct
    14581 tctggcgatc aaatgactgg tagagaacca aatcctctgc cacaatacag gcaaggtcgc
    14641 ccatggaacc attgtctcct cgcttgagca agacagcgct agtcagcttg aacaagagct
    14701 cctgatcaac agaaacatca tacagagcca gtaaagcctt gacaaccaag acaacgacgc
    14761 tgccactaga acctagacca aactttttcc cttctcgttc cattttgcca cagatttcta
    14821 gagaaaaagg tcttaaattc tgaccacgaa cagcgaggaa gtctcccatc aaagcaatcg
    14881 tttcttgaat caagctgtag tcaggattag gccttaagtc cactgcgaaa tcaaacatat
    14941 ctgaatagat acggtagctg tcagaaaaag caatctcagc cctcatatag atgagaatat
    15001 cctttatcaa agctaactgc cctggctcta aaatagcata ttcacctgcc caatagagtt
    15061 ttccgcaagt tttaacagca atcatcttga ctcaaatcct ttgtttttga cacaatcaag
    15121 cgataacgct gaccgaaaat ttctgacaaa tactccaagt ctttctcctg acagaagacc
    15181 ttaacattgg gaccagcatc catggtaaag tagcaggcct ctcctttctc acgaagctgg
    15241 cgaacaaagt ccatagcctc ataagaggca tccgtcagat aagaaaaggc tggactagca
    15301 gtctttgtcg tagcatgcat agccagggca tttttctccg ttaattctcc aatcttggca
    15361 aaatcatttt ccttgagata aatcagcata tcctgatagt ccttctcaga ctgacgaacc
    15421 cagtcgtcga aagtcgtcga ggtttccaca caaagtttca tcccgtcacg gctagagatt
    15481 ggttttttct tgtcctctag caccaacata atcatagcta gtttcaagtc tgtctctaca
    15541 gggtaaattt ctccactatc cttatcccag gctcctagtg gtccataaaa actccgagaa
    15601 gaagagcctg aggcaaattt ggcttcctgt gccaactgac ttctatccaa tccaagcttg
    15661 aaataagcat tacaagcctt gaccagggcg gacaaaccac tagaacttga ggacaggccc
    15721 gctgccgtag gcatattgtt ttgagtatcg atacggacaa agccctcacc agctggacga
    15781 taacggtcaa taatcttact catcttggca tgctcgacct cattttgtag ctgaccattg
    15841 atgtaaaatt cgtcagctgt tacattggct ggtaaaggcg acaaggtcgt ctctgtatac
    15901 atattttcca aagttagaga aatactgcta gtagcaggca ccatctcttt ttcttttttc
    15961 tttccccaat atttgataat agcaatattt gcgtaggaac gtactgttac aggctctcta
    16021 tccatgtctg aacagctcct ttctcttcta atctttctgc tagttcttgt gcgtgtgtca
    16081 aattggttac caaggctatg atacaacctc ctagcccacc accgctcatc ttggcaccca
    16141 gagcaccatg gctaagagtc gtttcaacca aaaagtctgc ctcagggcta ctgactccaa
    16201 tttcttttaa atgtaaatgc gcttgactga ggatttgtcc cagtccttca gcatcttttt
    16261 gtgaaatcgc aacttctgct tgctgggtta attctcccaa ggcatgcaaa aacggtaggg
    16321 catccttgcc cttattttga accacttgga tggcttcacg agtatgacca taaacacccg
    16381 tatcggcaat caccaaatag gcggataaat ccatctcaag ttctgtaaat cctacgttct
    16441 tgataaagcg aataggttgg tcactgagac aggtcttagc atccaaacca ctaggattca
    16501 tatgagcaat catctcagct cgattgacca agatttctag tacatcatga ggcagatcag
    16561 cctgatagta gtcaaatacc gcacgaatgg ccgctatgct gatagccgct gacgaaccca
    16621 tcccccgttt ctcagggata gccgagtcaa tctcacaacg aacgcaggtt tctgtgatat
    16681 ccaaatactc cagtgaagca taaaccgcca tggacaaggt atcctcctca taaaggcgcc
    16741 aaggactctc tgcaggaact accttacagg tcacctccac ctccaaaaga ggcagggaaa
    16801 tggcaggata accgtaaacg accgcatgtt cccctattaa aattatctta ctatgtgcct
    16861 gaccgacacc aacttttttt gtcatttttt ccttttacta gacgaaaaaa cgtcttattt
    16921 ttcatacaag tattaattct ttcctatcta ttttattata ttttcacaaa aaaagcgatt
    16981 gtttccattc acaatcgctt ctttcattat tgaacccatt cgccattata attgacaaaa
    17041 tagccatcta cggtcgtatt cactgccaag gcacctgagc tataagcgta gtaccatctg
    17101 ccattgacct ggaaccaacc tgtcgtcata gaacgacgaa agaaactcca taccattaag
    17161 taaagaggaa agtcgtgagg gagcatgcgc cattgacaac ctgttttagt gacgtacaaa
    17221 gtctcattaa caagtactcg tttcggtcat ttataggtgc ggtgtttgga gaaatagggt
    17281 tcaatcttag cccattcttg atcgtttaaa tcagtatcat atactttgcg tgtcataact
    17341 ctagtttaac attttttgtg aatacaggtt ctaaataatc gaccacgaaa atttcttaag
    17401 tggaaaacgc cttatgaagt atgctacggg aaagttatgc acttaatttg acaattcaag
    17461 atgtaaaaat at
//
LOCUS       DAVKFH010000033        17334 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKFH010000033 DAVKFH010000000
VERSION     DAVKFH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.033"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..1164
                     /locus_tag="VW006_001318"
                     /pseudo
     CDS             1..1164
                     /locus_tag="VW006_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1263..2885)
                     /gene="groL"
                     /locus_tag="VW006_001319"
     CDS             complement(1263..2885)
                     /gene="groL"
                     /locus_tag="VW006_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9380168.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2901..3185)
                     /gene="groES"
                     /locus_tag="VW006_001320"
     CDS             complement(2901..3185)
                     /gene="groES"
                     /locus_tag="VW006_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9380169.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3341..3736)
                     /locus_tag="VW006_001321"
     CDS             complement(3341..3736)
                     /locus_tag="VW006_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9380170.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3814..4575)
                     /locus_tag="VW006_001322"
     CDS             complement(3814..4575)
                     /locus_tag="VW006_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9380171.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(4617..5243)
                     /locus_tag="VW006_001323"
     CDS             complement(4617..5243)
                     /locus_tag="VW006_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9380172.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(5259..5576)
                     /locus_tag="VW006_001324"
     CDS             complement(5259..5576)
                     /locus_tag="VW006_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9380173.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5573..5872)
                     /locus_tag="VW006_001325"
     CDS             complement(5573..5872)
                     /locus_tag="VW006_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9380174.1"
                     /translation="MNGMKAKKMWMAGLALLGIGNLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(6157..6348)
                     /locus_tag="VW006_001326"
     CDS             complement(6157..6348)
                     /locus_tag="VW006_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9380175.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6509..7183)
                     /locus_tag="VW006_001327"
     CDS             complement(6509..7183)
                     /locus_tag="VW006_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380176.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(7189..7635)
                     /locus_tag="VW006_001328"
     CDS             complement(7189..7635)
                     /locus_tag="VW006_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380177.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPCYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7749..8252)
                     /locus_tag="VW006_001329"
     CDS             complement(7749..8252)
                     /locus_tag="VW006_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9380178.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8249..8611)
                     /locus_tag="VW006_001330"
     CDS             complement(8249..8611)
                     /locus_tag="VW006_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9380179.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8677..10536)
                     /locus_tag="VW006_001331"
                     /pseudo
     CDS             complement(8677..10536)
                     /locus_tag="VW006_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10575..11024)
                     /locus_tag="VW006_001332"
                     /pseudo
     CDS             complement(10575..11024)
                     /locus_tag="VW006_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(11148..11864)
                     /locus_tag="VW006_001333"
     CDS             complement(11148..11864)
                     /locus_tag="VW006_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9380180.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12745..14160)
                     /gene="ply"
                     /locus_tag="VW006_001334"
     CDS             complement(12745..14160)
                     /gene="ply"
                     /locus_tag="VW006_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9380181.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14171..14503)
                     /locus_tag="VW006_001335"
     CDS             complement(14171..14503)
                     /locus_tag="VW006_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380182.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTGIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14574..15182)
                     /locus_tag="VW006_001336"
     CDS             complement(14574..15182)
                     /locus_tag="VW006_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380183.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(15195..15653)
                     /locus_tag="VW006_001337"
     CDS             complement(15195..15653)
                     /locus_tag="VW006_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9380184.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15778..16177
                     /locus_tag="VW006_001338"
                     /pseudo
     CDS             15778..16177
                     /locus_tag="VW006_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16942..17202)
                     /locus_tag="VW006_001339"
     CDS             complement(16942..17202)
                     /locus_tag="VW006_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380185.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
BASE COUNT         5137 a         3929 c         3101 g         5167 t
ORIGIN      
        1 ccaattgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg aaacgactgg
       61 tatgcctact agaattctcc ttagaaagcg tcgattcaag tgttatcact gttcaaaaat
      121 gatggtcgct gaaacttcta tcgtcaagaa gaatcaccaa atccctcgta ttatcaacca
      181 aaaaattgcg caaaagttaa ttgaaaagat ttctatgacc gatattgccc atcagctttc
      241 catctcaact tcaactgtta ttcgtaagct caatgatttt cactttaaac atgatttttc
      301 ttgtcttcct gagattatgt cttgggatga gtatgctttt acaaagggaa agatgagttt
      361 cattgcgcaa gattttgata atcttaatat tatcactgtt cttgaaggca gaacacaagc
      421 tgtcatccga aatcactttc ttcgctacga tagagccgtc cgatgtcgcg tcaaaatcat
      481 tactatggat atgtttagcc cttactatga cttggctaaa cagcttcgct ttcaaatttc
      541 taggctcagg ctgaaacagt ctcccagact gtttcactcc cgaatgctaa aatcgttctt
      601 gatcgctttc acattgtaca acatcttagc cgtgctatga gtcgtgtgca tgtccaaatc
      661 atgaatcagt ttcatcgaaa atcccatgaa tacaaggcta tcaagcgcta ctggaaactc
      721 attcaacagg atagccgtaa actgagtgat aagcgatttt atcgtcctac ttttcgtatg
      781 cacttaacca ataaagagat tttaaacaag cttttgagct attcggaaga cttgaaacac
      841 cactatcagc tctatcagct cttgcttttt cacttccaga acaaggaacc agagaaattt
      901 ttcggactta ttgaggacaa tcttaagcag gttcatcctc tttttcagac tgtctttaaa
      961 accttcctca aagataaaga aaagattgtc aacgcccttc aactacacta ttctaatgcc
     1021 aaactggaag cgaccaataa tctcatcaaa cttatcaagc gcaatgcctt tggttttcgg
     1081 aactttgaaa acttcaaaaa acggattttt atcgccctga atatcaaaaa agaaaggacg
     1141 aaatttgtcc tttctcgagc ttagctgact tcaacccact acagttgaca aagagcctat
     1201 aaaaagaagg gttagtcatt ccctcctttt gtgtttttta taagttgttt tctatagaaa
     1261 gcttacatca tcccgcccat catacttgga tccattgctg gagctggggc tactggttct
     1321 ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc tggctacaga tgctgcattt
     1381 tgtagggctg aacgactcac tttaactgga tcaatgatac cttgatcaat catgttaacc
     1441 cactcgccag ttgctgcgtt aaatcctata ccaagctcag catttttcaa acgatcgata
     1501 acgatagatc cttcaaatcc tgcattgtga gcaatttgac gaacgggttc ttccaaagca
     1561 cggagaacaa tattacgtcc tgttgcttca tctcctgtca attccaaggt agcaacagct
     1621 ggaatcacat tggcaagagc tgttccaccg cctgcaacaa taccttcttc aacagctgca
     1681 cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt ctttcaactc agtttcagtt
     1741 gcggctccga ccttaataac cgctacacca cctgacaatt tggccaagcg ttcttgcaat
     1801 ttttcacggt caaattcaga agttgtagtt tcgatttgag acttgataac cgcaacacgg
     1861 tgagaaatcg cttcaggatt tcctgcacct tctacaataa ccgtgctatc tttgtccacg
     1921 gtcactctcg ctgcttgacc aagagcttca attgtcgcat ctttcaactc aagaccaagg
     1981 tcttctgtga taactgttcc gcctgttaag atggcgatat cttcaagcat ggctttgcga
     2041 cggtcaccaa aaccaggtgc cttgactgct actacgttga aggttccacg aatcttgttc
     2101 aaaacaagag ttggaagagc ctcgccatcc acatcatccg caataatcaa gagtggacga
     2161 ttgctttgga gaatgctttc caaaagtggc aagatctctt ggatattgga aattttcttg
     2221 tctgtaatca aaatgtacgg attttcaagg tcagccacca ttttttcgct atctgtcacc
     2281 atgtactgtg aaaggtaacc acggtcaaac tgcattcctt ctacgacttc aagctctgtt
     2341 tccataccac gtgactcttc gatggtgatg acaccgtctt tgccaacttt ttccattgct
     2401 tcagagatgt actcaccaac tttttcagaa cgagaagata cggctgcaac ttgagcgata
     2461 gcttctttat tggcaacagg gatggcgttg tttttcaaag cttcaactgc tgcggcaact
     2521 gctgtttcaa tcccacgacg aataccgatt ggatttgcac ctgctgtgac gtttttgatt
     2581 ccttcacgga cgatagcttg ggtcaagact gttgcagtcg tagtcccgtc acctgcgata
     2641 tcattggttt tagaagctac ttctgatacc aatttggcac ccatattttc aaaatggtct
     2701 tctaattcaa tttctttggc aatagtcaca ccgtcattgg taatcaatgg tgagccaaat
     2761 gatttttcaa gaacgacatt gcgacctttt ggtcccaagg ttactttaac agtatctgca
     2821 aggatatcga caccacggac catagctgaa cgggcatctg atgaaaattt aatttctttt
     2881 gacatactta ctttctcctt ctattcttca atgattgcca agatgttagc ttcgcctacg
     2941 atgatatact tttcatcgcc atctttgaca tcaagacctg cgtgggcttc aactaagaca
     3001 cggtctccag ttttaacact tggagcaacc aagtcaccgt tcaaggtacg aacaccttgt
     3061 ccagtagcca caacttgggc tgttttggtt ttttcttggg ctgaacctgc gagaacaaag
     3121 cctccaacag tttgttcttt ttcttctact tttaagacca aacggtcccc taatggtttc
     3181 aacatctgat ttcctccata atgagataga tattattagc actctttata cctgagtgct
     3241 aattcatagt tctattgtat cacttagtca gaaataatca agaaaaaagt ctgactttct
     3301 caagataaaa agcctgagac caactcagac tttttaattc ttaaaatggc aattcttcct
     3361 cttccaagac caaatctgcc aaatcttggc ctgcattatt ttcacgcata gcacgttggg
     3421 cacgactttc caagagttgg aatcctgtca caaggacttc ggttacataa ttcatctgac
     3481 catttttctc aaagcgacgg gtacgcaatt ctccatcaac ggaaatgaga ctacctttgg
     3541 ttgcgtagct tgccaaagtt tctgctagtc tgccccatag gaccatattg acaaaatcaa
     3601 cttcacgttc accgttttgg tctttgtaac gacggttgac agcgatagtt gctcgcgcta
     3661 ctgacttgtc attgttggtt ttgtgcaatt ctggtgtaga cgttaaacgt ccaatcaaga
     3721 taactttatt atacatattt tcttcctcct acttatctat tcgtaggaaa tcaaaaaaag
     3781 ttacagaaat ttgtaacttt tcgagaaaat tttttatttt ttatgaacca tgaaacctgt
     3841 cgcctgttga ttggccataa tggtcatatc tgtaatctga acacgacgag gttgactagt
     3901 cacatagact actgtatctg caatatcctg agcttgcaaa gcttctattc cttggtaaac
     3961 ggacgcagct cgttctttat caccatgaaa acgcactgta gaaaaatctg tttcgacaat
     4021 tccaggctga atggttgtca ccttgatatc cgttgcgatg gtatcaattc gcagtccatc
     4081 cgaaaaggtc ttaaccgcag ccttggtagc tgagtaaaca gcggcaccag cataggcgta
     4141 gattcctgcg gttgacccca tattgataat atgaccttga ttgactttta tcattgctgg
     4201 caagaaacag cgagtaaccg ccatcagacc tttaacattg gtatccaaca tggtcaacat
     4261 atccaactcc tcatagtctt gatagggagc caagtcaaga gccagtccgg cgttgttgac
     4321 caagatatca atcctcccta tcgtttctag aatatcagag caaacagtct tcaccatgac
     4381 catatccgtc acatctagtg gaaaagtcca gacggtttga tttggaaagc ttactgcaaa
     4441 ctccgacttg aggatttcta atctgtctat ccgtcgtcct gttagaacga catcctcacc
     4501 ctgctccaga taagcacgcg caatcgcttc accgattcct gaggttgctc ccgtaatcac
     4561 aacatttttt gccatcttat ttccttctag ctggtctatc agatattaac aacttcttag
     4621 gcagtccagt gtttagctga gtcaaatggt gttccgacaa cttggtcttc tgataattca
     4681 agcaccccac gtttttgtgg agcatttggc agatgcaatt cacgaggact gcacatcata
     4741 ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt tggcatcata
     4801 gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc tcctgcaacg
     4861 atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     4921 ggatgggcta ccatctcaac aatttcaccg acaacaaact taggttcctt atcattaaca
     4981 atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac ttgctcatct
     5041 gtcaaaaaga cttgaccgcg ctctgcagtt tcaaataaac ttgaaacttc gaaaatattc
     5101 caagccactg tttccccatt ttctttgagg aaaacacggg ctaccttgcc tttgcgttcc
     5161 acattcagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     5221 tctttgttat acgtaaaaat cattgtttcc tttttctcct atttcaatcc tgctaaaaaa
     5281 tcgttaattt gctccttact tttacggtcg cgattgacaa aacgaccgat ttccttgtcc
     5341 ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc caaatccata
     5401 tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcttc aatctctggt
     5461 aaggcaggat aaatataacg acaatcgcca caccagtctg ccacaaaaag gaagaccttc
     5521 ttgcccgctt tttccactaa agatgctaat tcttctaaac ttgctggctg tatcataaga
     5581 cttcctcctc atagactagg tcttcatttt catagacaaa ggtataatga cggccatcct
     5641 caaaaatgac gccaccaacc aagctctcca gactgctttc gtaaacttga acataaaggg
     5701 tcgcaatttc ccccatgtcg gaaaaatggt ctcgcacaat ctctgtcaac tcttcctgag
     5761 tcttcatgag cttacggtca tctgcaactt ttttcgtagc aagggcaagg tttccgatac
     5821 ctagcagagc caagcctgcc atccacattt ttttagcttt cataccattc attttaacac
     5881 aaaaaaggct tcaggacaaa tgaggaagca gcagaaaagc aagtaaaaag cctcttcctt
     5941 taaggaaaag gacttcttat actaagagca aactagaaag ctagccgcag gctgctcaaa
     6001 acacggtttt gaggttgtag ataaggcgac gttgacgtgg tttgaagaga ttttcaaaga
     6061 gtattattct tattgccagg cacctaagtt gccaacgtag taaccatcag gtgtgtaggt
     6121 attgcgaagc atcttacctg atgaagccag ataatactac ttgccattgt ctttgaccca
     6181 atcattcgca atcatggaac cagaagaact tacataatac cattctccct tgtcataaac
     6241 ccaagtactg actttcatgg ttcctgagca attaaaggca aaaaaactgt ccaatatcat
     6301 tcgtttttta aaagcattcg acactacatc atctacgctg tagtacatag atacatcaaa
     6361 cgtaaaagcc aaaatatcga aaaatataca acttttaaag gcagcatttc attttttaaa
     6421 gtctaacaac catagaactg aaatcagagc aaggcatcaa tttcctactc tatagtacgc
     6481 ctattttgat ggttgaagtg aagatttttt atttaacgta gacaatttcc aacttcccaa
     6541 aagccacatc tccacggata atcaaggttt tgctggttgg ggctacagga gcgtcagcct
     6601 tagctgcacc aaaggaggtt tctactttca aatctacacg ccagtgttgt ggaacataga
     6661 cggttgcatt tccgaaggct acttcaatat ttaaagttgc aaaatcacct agcatctctg
     6721 cattatcata gtagatttta gcatccccaa aagcgacttc cacttggtca tctacgagat
     6781 cttgatcttg cttatagaag gtcccgctac caaaagcgac ttccttatcc gtgacgactg
     6841 tttttttccc attgtaccac catttttttt cattccagaa cttacttgaa tgcgtcagat
     6901 aaccaacacc aagtaccacc aagatgctag cccaaataag agaatgattg gtaactggta
     6961 acaagtcata agcgtaattt gcaatgatga gcgccagtaa acctgtaaaa actgccgaag
     7021 tgagatgacg tctaaggatg gactcaatgg acttataagc aaaaaaaaca atacctagta
     7081 aaggccatat tttaccatcc aaagaaggaa ttccaaaatt cccttgcagc aagatccaag
     7141 ctgctaaaac caataaaaca ataccaaatg ctttcttttt catgtttttt acctcatgtt
     7201 tcttagattt tcttttagga gggattggta atgccgtgag acatgaacct ccttgtgcgt
     7261 ctgataaaag gaaatggtgc ccgttcctga aaaggacttg tccactgagt aaatctgacg
     7321 gatgtttgcg atcgttgact tggaaactcg attaaaatag caaggcaaga tagactccaa
     7381 ctcatagagc ttgaggcgaa cctcataagc ttccttctgg ttatgagcgt agatcttgct
     7441 cccttctgtt tcaaagaaga gaatttctga caggtctaga taatattcac ctgtcccctt
     7501 gtaaaaagtc aacctcggag cctttgactc ttgcaagagc cgttgcaagt ctgcaatttc
     7561 atctgtcaaa gtgggggtct tgatgacaat ttcagtctcc tctaaattgc cgtcaatctc
     7621 aattcgtaac ttcatcacat ctcctttctg actctactat atcaaagcta agttaggaat
     7681 acaagggcaa aaaagcaagt ggtcaatttg aagcagtaag tggttgtagg actagcttaa
     7741 cttacatgtc agaataggaa gaaaaggaat ccccacacca aaccacaagc atagccaacc
     7801 aagacatcct tgggataatg aacaccccct aaaaccctca ccaaccccag caaagctgaa
     7861 aagatcaagc atactaagcc aataggtaga ctagcatgca gacaggccat ggagatgata
     7921 gttgccgaaa agacatggcg actgggcatc gaccgtcccg aactatcctt atcaagaagg
     7981 ggacagatat cccaagtttc ataaggtctc gggtgattga ttttcttacg aaacagggac
     8041 aaggtcacaa aacctgatgc aggaataagc acataaaccc caacttgctg accaagtcct
     8101 agttgcaggt aagtggtgac tagcaagatc agatagacca ggggcatggc gactgtcatc
     8161 aaacgattga aactgcgtaa taggaataaa agaattggac gactagttag tctggagctg
     8221 atatttcgat accattcttg ataatttttc atttatgctt ctcatacttt tctagaactt
     8281 gttgagatgt tttccccttg gcaagccaat ccactagctt gtccaagcga cgtatttcct
     8341 gcatcagagg atcatcaatt tcctcaatac ggacaccgca aatcttccct gtaattgcaa
     8401 ttcgttcagg gacataatat ggtgcttgtc taaaaaaatc tccgtaagta acatccctat
     8461 ccagacaagc aagaacatca ctcacttcat accctgtcaa ccaactggtc acttggtgaa
     8521 ctgactctgc ctttccaccc tttcgctcaa cttttgcaat caaggctaag tagacagcag
     8581 caaatttcat attgtataat ttctgactca tacttctctc tttctatgtg tactagtata
     8641 aataagaaaa agaaggccgt caagccttct tttgatttat tcttctgctt catcttctgt
     8701 aaattgacta ttgtacaagt cagcgtagaa gccaccttgc gccatcagtt cctcatagtt
     8761 gccttgctcg atgatatttc catctttcat gaccaagatc aagtctgcat ttcggatggt
     8821 tgacaagcgg tgggcaatga caaaggatgt gcgtcattcc atcaaacggt ccatggcttt
     8881 ttggatcaat tcctctgtcc gtgtgtcaac agaagaagtc gcctcatcca aaatcaaaag
     8941 cggtgcatcc ttaagaaggg cacgagcaat agtcaatagt tgtttttgtc ctacagacaa
     9001 ggtcacggtg tcatccaaga tggtatcata gccatctggc aaggtcataa taaagtggtg
     9061 aattcccaca gccttactag cttccatcat tcgttcatca ctaatcccta tttgattata
     9121 gatgagattg tctcgaatag ttccttcaaa gagccaggta tcctgcaaga ccattgaaaa
     9181 ggcatcatgc acttctgaac gcgtcatagc tttggtatcc acaccatcaa tgtgaatact
     9241 tcccttatca atctcataga atttcatcaa aagattgaca atggttgtct taccagtccc
     9301 agtcggccca acaatggcaa ccttttgacc agcatgagct gtcgcagaga agtcatagtc
     9361 ttgaacattg acaccgtcca ccagaatttc tcctgctgac acgtcgtaga aacgtggaat
     9421 cagattgacc agagttgatt taccagaacc tgttgaccca ataaaggcca ctgtttgacc
     9481 agtttctgct ttaaagctaa catgttcaat aactgcctcc gaatttgccg catagcggaa
     9541 ggtcacatcc ttaaactcga cctgaccttt gaagttttca tcagtcagct gcacttgaac
     9601 agggttttgg atagaagaat gcaaatctaa aacttgatta atccgcttag cagagaccat
     9661 agttcgggga agaacgatga agagtgctcc catgagaagg aagcccatga caacctacat
     9721 ggcataagac atgaaaacaa tcatgtcact aaagagaggc agacgcgcta tcgaagcagc
     9781 gtcgttaatc acataggccc caatccagta aatcgctaca ctcaaaccac ttgaaatccc
     9841 catcatgata ggattcaaaa tagccataag acggttgaca aacaaattca aacgggtcaa
     9901 ttcatcattt actgctgcaa atttttcatt ttgataatcc tctgcattgt aggcacgaac
     9961 gacacgaata cctgttaaac tctcacgagt gatactgttc agtttatctg tcaacccctg
    10021 aatcaaggac tgttttggaa aggctagcgt catcaaaacg gtcgtcatca ggacgttgac
    10081 aatcactacc acaagtacgg cccagagtca gtattctgaa tgacctaaaa tcttcccaat
    10141 agcccagata gccataattg gaccacgcgt taccacttgc aagcccatag taatcaacat
    10201 ttgaacttga gtaatgtcat tggtagtacg cgtcaagagg ctaggaattg aaaatttctt
    10261 aatctctgtc tgcgagtaat ccaaaactcg gttaaaaata tcacttctca gcctactagt
    10321 ataagaagcc gccactcggg atgcaaaaaa tccaactgca actacggaca agaaggcaag
    10381 aaagagcatt cccatcatca tgcttgccgg ctgccacaac tcatctaaat tagtttcttg
    10441 actacctagc aaatccgtaa ttttcgagat ataggtcggc acttccaact ctagatagac
    10501 cgaaaagcaa gtaaagaaaa tggctagtaa aatcatcccc cattcttttc tactaattct
    10561 tttggttaat ttctttattc tctcctccta ttcccttgat attttgcctg tagttgaccg
    10621 agaactttct caaaaatcag taattcatct tcatcaatgt cttccatcaa ctgcttgtct
    10681 atgcgttcaa aaaaagcctt aacctgttgc atctgagaac gtgctttgtc cgtcagacga
    10741 acaaacttag cccgcttatc aacaggactc gcctccaatt ccaccaaacc attttgcact
    10801 atacgcttaa ccagattact agcaacagac ttggtaatat tgagttcctg ctcgatatct
    10861 ttaatcaaga ccaagtcttg gtttttctag cgattatcca aaaaacgcac aacctgacct
    10921 tgcggcccac ccataaattc aatgccgcaa cgtttggctt ccttttgcac catcaggtga
    10981 atttgatgac caaaacgctt aaagactaac atcggtttat ccataatctc ccccttctaa
    11041 ataaaaatag ttctctggag aataattaaa tttctatgag aactattttc ttgattaaaa
    11101 aaatcccaag tgattttctc acttaggatc atgttctata ggttaaatta aaacccatct
    11161 acgttcgtat aaatcttttg gacgtcttcg tcgtcttcaa gaacgctgta aagtttttca
    11221 aaggtttcaa ggtcttcgcc tgacaattcc acttctgact gaggaatcat ttccaattca
    11281 gtcacttgga attcttcaat accagactca cggagagcaa cgatagcctt gtgaaggtca
    11341 gttggcgctg tgtaaactgt gattgtacct tcttgtgctt ctacgtcatc cacatccaca
    11401 tccgcttcga gcaattgctc aaagactgcg tctgcatctt cacctccaaa tacaataaca
    11461 cctttgttgt caaagaggta agaaacagaa cctgaagcgc ccatgtttcc gccgttttta
    11521 ccaaaggctg cacggacatt ggctgctgta cggttgacgt tagaagtcaa agtatccaca
    11581 attagcatag agccatttgg cccaaaacct tcgtaacgtc cttctgtaaa ggtttcgtct
    11641 gtgtttcctt tggctttatc aatcgcttta tcgataatgt gttttggcac ttgggcttgt
    11701 ttagcacggt cgataacgaa tttcaaagct gagtttgatt ctggatctgg atcacctttt
    11761 ttagctgcta catagatttc tacaccaaat tttgcatata ccttagagtt agctccatct
    11821 ttagccgttt tcttggctac gatattggcc catttacgtc ccattaggaa tctccttttt
    11881 tcacatttta atctttatta ttataacaca agtttttttg attttcacta gaggaaatgg
    11941 attttattag caaatcaagc taggatagca ctttacctgc taagatggtc ttacctttct
    12001 atctttatca acagacactc atccacattc aaaaaacaaa ctagaccatt atctgcaaat
    12061 agaaagtttc agccaagttt gacaaagtca gctcaaatta ctgtttgaag tttgtagata
    12121 taagcgacaa aaacaatcat actgcacctt ttgttgacag tctactccag acatatcata
    12181 gttcaagtaa atactttgaa attcaacagt tcttataggc gctattgtat tctaagaaat
    12241 caatagaaga gtttctaagc aaacctctaa tactcaataa aaatcaaaga gcaaaccaga
    12301 aagctagccg caggttgctc aaaacactgt tttgaggttg cggatggggc tgacatggtt
    12361 tgaagagatt ttcgaagagt ataatttacg tgttcccaag atggagaagt tagactagta
    12421 cactggcact tctaaaccat tgctagcaat tgatttgttc atatttaatt tcattttttc
    12481 cataaatggg tattagatat aaacagcaaa atatttccga tacgtgtcgt tcttgaattt
    12541 ccaatcatct aaaacaagta aaggataatc aatcccctgt atatcaagga attggctacc
    12601 ctttttactt ttttacacat tctgtttgat agattcattt taacatcacg agcatactcc
    12661 aatggaaatc gctaggcaag agataaactt tcagatatcc gcagagagat catcgcctct
    12721 ttttgtcgca agcattctcc tctcctagtc attttctacc ttatcttcta cctgaggata
    12781 gagagttgtt ccccaaatag aaatcgtccg cttacgcact agtggcaaat cggttttttc
    12841 ataaaccgta cgccaccatt cccaggcaag cccggtacac tctctaattt tgacagagag
    12901 attacgaaca ttccctttta aaggaatact agtggtaaag tgagccgtta aatcctgccc
    12961 atttctgtcc caagccttag gagtcaagac ttccttacct tgatgatcat aggataattc
    13021 attccaagta atataatatt gggcaacata ggcaccacta tgatccagca gtaaatctcc
    13081 gtttctgtaa gctgtaacct tagtctcaac atagtctgta ctattttgaa aggtcgcaac
    13141 tacattgtca cgtaaaaaag aagttgtata ggaaatcggc aagcctggat gatctgctgt
    13201 aaagcgactg ccttcttgaa tcaagtcctc taccatatcc accttgcctg ttacaactcg
    13261 ggcacccgaa cttgggtcgc cccctaaaat aaccgccttc acttctgtat tgtccaaaat
    13321 ctgtttccac tctgtctgag gagctacctt gactcctttt atcaaagctt caaaagcagc
    13381 ctctacttca tcactcttac tcgtggtttc caacttgaga tagacttggc gcccataagc
    13441 aacactcgaa atatagacca aaggacgctc tgcagaaatt cctctctgtt ttaaatcctc
    13501 taccgttaca gtatcttgaa acacatctcc tggattttta acagcatcta cgctgactgt
    13561 ataataaatc tgcttaaaat taacaatctg aatctgcttt tcgcccgaat ggacagagtt
    13621 aaaatcaata tcaagagaat tccctgtctt ttcaaagtca gaaccaaact tgaccttgag
    13681 ttgttccatg ctgtgagccg tgattttttc atactgcatt ctagctggga cattattgac
    13741 ctgaccataa tcttgatgcc acttagccaa caaatcgttt accgctccgc gaacacttga
    13801 attgctgggg tcttccactt ggagaaagct atcgctactt gccaaaccag gcaaatcaat
    13861 actataagtc atcggagcac gatcgaccgc aagaagagtg ggattattct ctaacaaggt
    13921 ctcatccact acgagaagtg ctccaggata gaggcgactg tcgttggtag ctgttacaga
    13981 aatatcactt gtatttgtcg acaagctccg cttctttctt tcgataacaa caaactcatc
    14041 gggtagctga ttaccctctt tgatgaaacg attttcaata ctttctccct gatgggtcaa
    14101 gagtttcttt ttatcgtaat tcatagctag tataaagtca tttactgctt tatttgccat
    14161 cttctacctc ctaataagtt cctggattga gttgcataaa ctcagacttg ttcagcgaaa
    14221 tcagccgtgg ttggactaag taatccaaaa tttcctcgta caattcttct gagacattgc
    14281 gtcgccgtct ggctagataa gaagtcggaa tgcccgtatt atccaacata aataccttat
    14341 ctaagtcaat caaggttggt cttgtaaaag gattacgagc tagatccggc tcttctatca
    14401 taaagttctt gaccaaacgt ctggtcaaga gagctggttt gaaggtctga tttttaacca
    14461 actctttgtt tttagtcatg ctgttgtcaa tacagatata catatgattc ttcacagcca
    14521 aatcgctact aatagtcgga aaaggcaaat aaagagctac aacatctcct ctcttaatca
    14581 agcaagagca cccccttttc tcctaatgta acatagacag gattgaccaa gtcttctgat
    14641 tgactcagaa tttccaaagt ttgagtttgg cgcgctgtca atttagtagc atcttgtctc
    14701 ttcaatacaa aatgcttgtc gccaataacc ttgacaatat aatccttctc caaagctgac
    14761 tggtaaatcc acatcagatg ttgtctgtcc tgagaactca agagagaagg attttcaagc
    14821 ctcccgatag tctgataaaa atcaaaaaca ggagctaact cctgccaatc tgattggcta
    14881 gttgtcaagg ctagaaaaag ggctttgcga gctgatactt cttggttagc cttgagagtt
    14941 actttcccct ccaagttttt tagaaatcgg gaaactccag aaagcaaatt tttctctaac
    15001 tgcgagaaat aaaaaccttt cgttcccaga cataagtctt tcatgtcgct ttctctagca
    15061 aataagagct caaacatttg atagtaaaag aaaaatatct ggcactgggt cgcgctcatc
    15121 ttttccttat cggcttcttt ttttaaccag agcaagggcg acaggtaact ggattgagac
    15181 atttcctcta cctcctactc ttttttaact ggagcatctg cactagctgc cacttctttt
    15241 gactggatac tttcccactg gttaatctcc tctgagataa gaccttcgca tgtcttgaca
    15301 aatagggcaa aagccttggt ctttcctgca tatttctccg tttggcattg atagaggaat
    15361 ttttctttct ccaggagttg cgcagttttt tggtaagaaa tccaattttc ctttgcatta
    15421 tacaaattga taatcccctc acacagcaag ccgagactgg ataaggcaac cgaaatcaaa
    15481 cggtagcgat cacctggcat aggaatagca caaaagacag ctatgaggaa acctgccacg
    15541 atttctgtta tttttaatac cttatagcgc ctacgatgtt gaacgctttt ctttaaaaaa
    15601 tgagctatct gtacgtctaa tcgctctgtc aggtacattt cttctggcgt catattcgta
    15661 actcctttca tttactttga taaccatagt ataacatatt tgaatatcaa agtatagtaa
    15721 aaactggact ataacttatt agacttcctg cgaaacaaaa tatggtatag tagttctatg
    15781 aattatgaag caagtaaaca actaactgat gcacgattta agcgtcttgt tagtgttcag
    15841 cgtaccactt ttaaagagat gttagctgta ttaaaaacag cttatcaaaa aagtcggaca
    15901 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc
    15961 acaaattcca cgtaaatcca gcaaactcaa gccgctaaca gttgaagata aagcctgtaa
    16021 ccatgcgcta tctaaggaga gaagcaaggt tgagaacatc tttgtcaaag taaaaacgtt
    16081 taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc ttcggattac gaatgaattt
    16141 gattgctggt attatcaatc ataaactagg attctagttt tgcaggaagt ctattatata
    16201 tatgcacaga catatgaaat ctttttgata cagccttctt tttacataga gagacagcag
    16261 ttatgatata tttatccatt cacaaaatct tctgtaccaa tactacaata attggtacat
    16321 tttgattaca caatcgtttc aaaaaatatg atggaagact gagtatttta tagtaattcc
    16381 cttcctatca actagatcaa ccagttgagt gttgaatgtt gaaacaccct agcctcagca
    16441 aaggactatg atcagcattt cctagccaat aggacgctcc aaatcaccta ttctaagtcc
    16501 atcactagct tggtcagtaa gatacagact gcaaggcaag actgacgtgg ttgatgagat
    16561 tttggaagag tattacaatc atagattcac cagtagactt gaccgcagat ttaactatag
    16621 tagattgaaa ctagaatagt acacttctgc ttctaaaaca ttgttagaaa tcgatttgac
    16681 tgtcctgatc aatttgtcct gttctcgttt cattttacta tactaaaaaa ccacctgaaa
    16741 caaatcaagt gactagagga taaaaacaaa gactgggcaa aaagttttga aaagcaaaaa
    16801 acgcataata tcaggtcttg aaaaaccttg ctactatacg tttgattgtg ggaagattga
    16861 cttcattttc tcctgaaatt gaagtttttt ctagtttcca ggtatagacg aattcaatat
    16921 agccatctcg ctacaattat tttaaccatc gaaaaaattt ttcagaattt caaaaatttc
    16981 attttgatcc aatgaaatgt cattattttg aagaatagtt gacttcacag gctctaattc
    17041 tatcacaaag cggtgcatgt ctgcttttac cgcactactt gatgttatag ccgataactt
    17101 ttcatctcct agcaaggaag ctgtcaaacg ggtaaggtca ttcaaatgct ttttaatgga
    17161 cttacttaaa ccttgagcac ctgtggcaga gcgctccctc atatccagcc aagctttagc
    17221 cttaaaaata attaatactc aatgaaaatc aaagagcaaa ctaggaagct agttgcaggc
    17281 tgctcaaaac actgttttga ggttgtagat ggaagctgac gtggtttgaa gaga
//
LOCUS       DAVKFH010000034        17007 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKFH010000034 DAVKFH010000000
VERSION     DAVKFH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.034"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            335..1381
                     /gene="pheS"
                     /locus_tag="VW006_001340"
     CDS             335..1381
                     /gene="pheS"
                     /locus_tag="VW006_001340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9380186.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1381..1890
                     /locus_tag="VW006_001341"
     CDS             1381..1890
                     /locus_tag="VW006_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380187.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1967..4369
                     /locus_tag="VW006_001342"
     CDS             1967..4369
                     /locus_tag="VW006_001342"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9380188.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4437..5438)
                     /locus_tag="VW006_001343"
     CDS             complement(4437..5438)
                     /locus_tag="VW006_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9380189.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5594..6040
                     /locus_tag="VW006_001344"
                     /pseudo
     CDS             5594..6040
                     /locus_tag="VW006_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6166..6432
                     /locus_tag="VW006_001345"
     CDS             6166..6432
                     /locus_tag="VW006_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380190.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6693..8942
                     /gene="metE"
                     /locus_tag="VW006_001346"
     CDS             6693..8942
                     /gene="metE"
                     /locus_tag="VW006_001346"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9380191.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9006..9872
                     /gene="metF"
                     /locus_tag="VW006_001347"
     CDS             9006..9872
                     /gene="metF"
                     /locus_tag="VW006_001347"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9380192.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10557..12770
                     /gene="pnp"
                     /locus_tag="VW006_001348"
     CDS             10557..12770
                     /gene="pnp"
                     /locus_tag="VW006_001348"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9380193.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDLEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            12786..13403
                     /gene="cysE"
                     /locus_tag="VW006_001349"
     CDS             12786..13403
                     /gene="cysE"
                     /locus_tag="VW006_001349"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9380194.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13415..14299
                     /locus_tag="VW006_001350"
     CDS             13415..14299
                     /locus_tag="VW006_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380195.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14386..15729
                     /locus_tag="VW006_001351"
     CDS             14386..15729
                     /locus_tag="VW006_001351"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9380196.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15722..16108
                     /locus_tag="VW006_001352"
     CDS             15722..16108
                     /locus_tag="VW006_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9380197.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16112..16996
                     /locus_tag="VW006_001353"
     CDS             16112..16996
                     /locus_tag="VW006_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9380198.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         5048 a         3323 c         3894 g         4742 t
ORIGIN      
        1 gttttcatct tatttcaatc cgctataaag ttagataaga ataagctaac aagcagaagt
       61 aagataggtc ttgcaagaat ggtaaaaatc tgataaacta agtggtaatt gaatagaaat
      121 ctgaaagact agtagcccaa gggaagtata tcagggagga gagccgtgac tgcaagctct
      181 ctatatgaaa gctgggtgaa ttcacttgcg catggattta gaaatgaagg tctgacttgt
      241 cagatgaaaa cggatggtac cgcgtgtcaa cgctccgagt ggagtttttg gcatgtggtt
      301 ttctttttat ctacgagaga ctgatggagg aaatatgtca actattgaag aacaattaaa
      361 agcgcttcgc gaagaaacgc tgactagctt gaagcagatt actgctggaa atgaaaaaga
      421 gatgcaagat ttgcgtgtct ctgtccttgg taaaaagggt tcgctcactg aaatcctcaa
      481 agggatgaaa gatgtttctg ctgagatgcg tccaatcatc gggaaacacg tcaatgaagc
      541 tcgtgatgtc ttgacagctg cttttgaaga aacagctaag ctcttggaag aaaagaaagt
      601 cgcggctcaa ctggctagcg agagtatcga tgtgacgctt ccaggtcgtc cagttgcgac
      661 tggtcaccgt cacgttttga cacaaaccag tgaagaaatc gaagatatct tcatcggtat
      721 gggttatcaa gtcgtggatg gttttgaagt ggagcaagac tactataact ttgaacgtat
      781 gaaccttcca aaagaccacc cagctcgtga tatgcaggat actttctata tcactgaaga
      841 aatcttgctc cgtacccaca cgtctccagt tcaggcgcgt gctatggatg cccatgattt
      901 ttctaaaggt cctttgaaga tgatctcgcc agggcgtgtc ttccgtcgcg atacggacga
      961 tgcgacccac agtcaccaat tccaccaaat cgaaggcttg gtagttggga aaaatatctc
     1021 tatggctgat cttcaaggaa cgcttcagtt gattgtccaa aaaatgtttg gtgaagagcg
     1081 tcaaattcgt ttgcgtccat cttacttccc attcacagag ccatctgttg aggtggatgt
     1141 ttcttgcttc aagtgtggtg gagaaggctg taacgtatgt aagaaaacag gttggatcga
     1201 aattatggga gccggtatgg ttcacccacg tgtccttgaa atgagtggta tcgatgcgac
     1261 tgtatactct ggttttgcct ttggtcttgg acaagagcgt gtagctatgc tccgttatgg
     1321 aatcaacgat atccgtggat tctaccaagg agatgtccgc ttctcagaac agtttaaata
     1381 atgattagaa aagtagaaat ggcagatgtt gaggtgttgg ctaaaattgc caaacaagcc
     1441 tttcgtgaaa cctttgcgta tgataatacg gaagagcagt tacaggaata ctttgaagag
     1501 gcttatagtc tgaaaacttt gtcaactgag ttgggaaatc ctgactctga aacctatttc
     1561 attatgcatg aggaggagat agctggtttt ctcaaagtca actggggaag tgctcaaact
     1621 gagagagaat tagaggacgc ttttgaaatt caacgcctct atgtgctaca aaaattccaa
     1681 gggtttggac taggtaagca actgtttgaa ttcgcacttg aacttgctac aaaaaatagt
     1741 ttttcttggg cttggctagg tgtttgggag cataatacaa aagctcaagc gttttataat
     1801 cgatatggtt ttgaaaaatt tagccaacat cattttatgg ttggtcaaaa agtagatacg
     1861 gattggttac tgagaaagaa attaaggtaa gaagaggatt cttctattga aatgatagga
     1921 aaggaaaaga accagtgtgg caatggtcat tctggagaac taaattatgc ttatatctta
     1981 taaatggtta aaagaattgg tggacattga tgtgccatca caagagttgg ctgaaaaaat
     2041 gtcaactaca ggaatcgagg tagagggtgt cgaatcacca gctgctggtc tctcaaaaat
     2101 tgtcgtcggt gaggtcttgt cttgcgaaga tgtgccagag actcacctcc atgtttgtca
     2161 ggttaacgtt ggcgaagaag agcgtcagat cgtttgcggt gccccaaatg tgcgtgctgg
     2221 gatcaaggtc atggtggctc ttccaggagc tcgtatcgct gataactaca aaatcaaaaa
     2281 aggaaaaatc cgtggtttgg agtcacttgg aatgatctgt tcacttggtg aattgggaat
     2341 ttctgactca gttgtgccta aggaattcgc agatggcatc caaatcttgc ctgaagatgc
     2401 cgtgccaggt gaggaagtct tttcttacct agacttggat gatgaaatca tcgaactttc
     2461 catcacacca aaccgtgcag atgccctttc tatgtgtgga gtggctcacg aagtggcagc
     2521 catctatgac aaggcagtca actttaaaga atttactcta acagaaacta atgaagctgc
     2581 ggcagatgcc ctttctgtca gcattgagac agacaaggcg ccttactatg cagctcgtat
     2641 cttggacaat gtgaccatcg caccaagtcc acaatggttg caaaatcttc tcatgaacga
     2701 aggaatccgt cccatcaata acgtagtgga cgtgaccaac tacatcctgc tctattttgg
     2761 tcaaccaatg catgcctttg acttggataa ctttgaaggg actgacatcc gtgtgcgtga
     2821 agcgcgtgct ggtgaaaaat tggtgacctt ggacggtgaa gaacgtgact tggacgtgaa
     2881 tgacctagtc atcactgtcg cagacaagcc agtagccctt gcaggtgtca tgggtggtca
     2941 agcaacagaa atctctgaaa aatctagtcg tgttgtcctt gaagctgctg ttttcaatgg
     3001 caaatctatc cgtaagacaa gtggtcgcct gaaccttcgt tctgagtcat cttctcgctt
     3061 tgaaaaagga attaatgtgg caacagttaa tgaagccctt gatgcggcag ctagcctgat
     3121 tgcggaactt gcaggtgcga cggtgcgtaa gggcatcgtt tcagcgggtg agcttgatac
     3181 ttcagatgta gaagtttctt caacccttgc tgatgttaat cgtgtcctcg gaactgagct
     3241 gtcttatgct gatgtagaag acgtcttccg tcgtcttggc tttggtcttt ctggaaatgc
     3301 agacagcttt acagtcagag tcccacgtcg tcgttgggat atcacaatcg aagctgacct
     3361 ctttgaagaa attgctcgta tctatggtta tgaccgcttg ccaactagtc taccaaaaga
     3421 cgatggtaca gcaggtgaat tgacagccac acaaaaactc cgccgtcaag ttcgtactat
     3481 tgctgaagga gcaggtttga cagaaatcat cacctatact ctaacaactc ctgaaaaagc
     3541 agttgagttt acggctcaac caagtaacct tacggaactc atgtggccaa tgactgtgga
     3601 tcgttcagtc ctccgtcaaa atatgatttc aggtatcctt gataccgttg cctacaacgt
     3661 ggctcgtaag aataaaaact tggcccttta cgagattgga aaagtctttg aacaaacagg
     3721 taatccaaaa gaagaacttc caaatgaaat caacagtttt gcctttgcct tgacaggctt
     3781 ggttgctgaa aaagatttcc aaacagcagc agttccagtt gatttcttct atgctaaggg
     3841 aatccttgaa gccctattta ctcgtttggg actccaagta acctatacag caacatctga
     3901 aatcgctagc cttcatccag gtcgtacagc cgtgatttca ttcggtgacc aagttcttgg
     3961 tttccttggc caagtgcatc cagtcactgc caaggcttac gatattccag aaacgtatgt
     4021 ggctgagctt aacctttcag ctatcgaagc tgcgcttcag ccagcgactc catttgtaga
     4081 aatcaccaaa ttcccggcag tcagccgtga cgttgccctt ctcctcaagg cagaagtgac
     4141 tcatcaagaa gttgtagatg ctatccaagc tgccggcgtg aaacgtttga cagatatcaa
     4201 actctttgac gtcttctcag gtgagaaatt gggacttggt atgaagtcaa tggcttatag
     4261 cttgaccttc caaaatccag aagatagctt aacggacgaa gaagtcgcac gctatatgga
     4321 aaaaatccaa gcatcgctcg aagaaaaagt caatgcagaa gtgcgttaaa cgatagtatg
     4381 atacaaattc cccctctagt ttatgaacac tagaggggtg attttatata aaataattaa
     4441 aatgtgattt ctgtaaaaat acatctatga tcagagtttt gaaaacttac aatgtctaaa
     4501 tctttaacat agtagtggtt tttaggcaat aaaatatgat ctattgttgc attaaaaagt
     4561 tttggacttt ggctattcca agttcctctt tcaaaaggtg gcagtacatt taatgcgtcc
     4621 ctatgagagc ttatttttgc aagtgctcca tgacgcatag ttgcattaaa atcacctgca
     4681 ataatagcct ttggatattt tgaagccaat tgattatgaa tgatgtttaa gtcttgcttc
     4741 cagatttcca ttaaacctgg cagaggagga gcagtatgca aggcaatgat atctggtata
     4801 ttttgttttc tcgaatgtaa tacaattgtc ccgaaccgtg ttgtatgaaa agttttagct
     4861 tctgtataga aaccataact tttcttgaca atcacagtca caggagctat tccactattg
     4921 gtaggtggag aagtgaaaat atcatagttg gccatagaaa gtccaacttg atgaaacaat
     4981 agtttgattc tctggttttc ttgctcacct ctgatattgg tagctagttc agggaatata
     5041 gccatatcgg cgtcaaaatg gctaaaaatt cgctcaatat gttgtgcttc tatttgatta
     5101 gcgacgttcc aagttactaa ttttaaggtt ttagtcttat ttgatgcaga agaaagggtt
     5161 ttatctgttc cgaataatag taatatgagc gatagtaaga gcaaaatgcc agagtaaaac
     5221 cgtatttgtt tatttttctt gtaaaaagat agggaaaaaa atacgaaact taaaatagca
     5281 gccatagttg cagtagctaa ggggaaggca taaatctgtg ccaagatagg ttgggtagat
     5341 agccaaatgt taagattaga agatgggaaa aaagaataag ttataatagg gatagaaaag
     5401 agaatgaccg aaagggcaga gagaaatgtt ttaatcattg tgtgagtacc tcaatttgca
     5461 atggttgctc ggagctaatg tgactttgtg actttacttg attgatattc tttttcttgt
     5521 cttcctcata gattaagtac aacccaagta aaaaaagtcc aatttaagat tgaaatgatt
     5581 aaaaggcaca gaaatgcaag acaattatac tacaaaaggc aaacatttga caatcgatag
     5641 ccgtcgctta atcgaacgat ggaaaaaaga aggaaaatca aatagagaaa ttgcctctct
     5701 acttggaaaa gctcctcaaa ctatccatac tgaaatcaag tatgggacag tccgacaatg
     5761 tcttggaaaa gggcgcttca aagagattta ttctgccgac tacgctcaac agtcttatga
     5821 aaataatcgc aagcactcgg tcaagaaatc aagcttgact aagaaactaa aggaaaagat
     5881 tctccactat cataaccaaa aattttcgcc tgaaatgatg gtgatggcta aaggggttaa
     5941 cgtgggaatt tcaaccattt actattggat tcatcgtgga aaattggggt taagcaagca
     6001 ggatctgctt tatcctagaa aaggaaaagc gcttaagaaa taggctagca ccaactttaa
     6061 acccgctggt caatctatcg aatcataatt cgctcatttt tttaaaaata catagacgta
     6121 aaaaaaagag ggttaagtga tgaagaatag tgctattggg agtaattgga aggatgtcca
     6181 tctgagctct ttagcaagga ggaaatcctt gaaagtgata tgcgagtggc tatcatgagc
     6241 gagttgattg aggctaggta tgaacaagga atcagtcaga aaaagctgga agaagtcagt
     6301 ggaataagcc agcctgtcat agctaggatg gagacaggag agagcagtct tcagttggat
     6361 acggtcttaa aagttctaac cagtctagga aagacactag cagtcgttcc acttgaacag
     6421 gggaaaagtt gactagaatc agttatagtc ttttctaata acaggtcata gtcacttata
     6481 agaattacta ataacatgtt ttagtattct aattggaata tagctgtaaa gtaagaatta
     6541 caaaggattt gagacgtttg cttcaagtct ttttcttttg tccgaaacag atatatagtt
     6601 tcaaactata tcaaacccta attttttaca attatacaga cgctttcttt tctgttaaga
     6661 tagtttcaac aacaaatttt ggaggacaca tcatgtcaac tacaatcatc ggtttccctc
     6721 gtttgggcga attccgtgaa ttaaaattta caactgaaaa atactttaga aaagaaatct
     6781 cagaagaaga acttttagca gcagcaaaag acttgcgtgc taaacactgg aacattgtca
     6841 aagaaaaagg catcactgaa attccatcaa atgacttttc tcactatgat aacttcctag
     6901 atgcggcttt ccttttcaac gtggtgcctg catccgttca aaacttggac ttgtctgacc
     6961 ttgagcgcta cttcgctttg ggacgtggtt accagggaga aaaaggggac gttcgcgccc
     7021 ttccaatgaa gaaatggttc aacactaact accactacat cgttcctaaa tttgaaaaag
     7081 acactcaagt aaaattggct ggtcacaaga ttttcgatga gttccaagaa gcaaaagaac
     7141 ttggattgaa cactcgtcct gttcttgtag gtccattcac tttccttcaa ttgtcagact
     7201 ttgaagaagg cgtgaaagca gacgacttcg tagatagctt agtggttgct taccaagaag
     7261 tttttgctaa attggctgaa cttggtgcga ctcgcatcca attggatgaa gcggctcttg
     7321 taaaagactt gacagctgaa gaaaaagctc tcttcttgaa cctttacaac aaactcttgg
     7381 ctgacaaaaa aggtcttgaa gtcttgcttc aaacttattt cggtgatgtt cgtgacgtat
     7441 acgctgacct tgtaaacttg ccagtagatg ctatcggtct tgactttgtt gaaggtaaga
     7501 aaactcttga actcgttaaa ggtggcttcc cagctgacaa gactctctat gtaggtattg
     7561 tcaatggtaa aaacatctgg cgcaacaact acgaaaagag cttggctgtt cttgagcaaa
     7621 tcccagctga aaacatcgtt ttgacaagct cttgctcact tcttcatgtg ccatttacaa
     7681 ctgctaatga agaatttgaa ccagcactct tgaaccactt tgcctttgca gttgaaaaat
     7741 tggatgaaat ccgtgacttg gatgctatcc gcaatggtca aggttcagaa gctcttgcag
     7801 ccaacaaaga actctttgcg actgagcgtg ttggtgaaaa tgctgaactt cgtgcgcgta
     7861 tcgctggctt gacagacgca gactacactc gtttgccagc ctttgcagaa cgtgaagcta
     7921 tccaagaaga agctttcaaa cttccagctc ttccaacaac aacgattggt tcattccctc
     7981 aaacaaaaga agttcgtgcc aaacgtttgg cttaccgtaa aggtgaattg tctcaaaaag
     8041 agtacgatgc tttccttgct gaaacgattg acgaatggat caagtggcaa gaagatattg
     8101 actttgatgt ccttgttcac ggtgaatttg agcgtaatga catggttgag tacttcggtc
     8161 aaaacttgtc aggatacctc ttctctaaaa atggatgggt acaatcatac ggtatgcgtg
     8221 gggtaaaacc accaatcatc tggggtgatg tcactcgtct taaccctatc actgttaaat
     8281 ggtctagcta tgcacaaagt cgtacaaaca aacctgttaa aggtatgttg actggacctg
     8341 ttaccatcct taactggtca ttcccacgtg aagacatctc tatcaaggat tcaactcttc
     8401 aaatcgccct tgctatcaag gatgaagtac ttgaccttga agctgctggt gtgaaaatca
     8461 tccaaatcga cgaggctgct cttcgtgaaa aattgccact ccgccgtagc gactggtacg
     8521 aagactacct tgactgggct attcctgcct tccgcttggt acactcaaca gtagcgccag
     8581 acacacaaat ccacactcac atgtgttact cagaatttac agatatcatc ccagctatcg
     8641 ataacatgga tgcagacgtt atttcctttg aagctagccg ttcaaacctt gaaatcttgg
     8701 acgaactcaa agcgaaaaac ttccaaacag aagtgggacc tggggtttac gatatccact
     8761 cacctcgtgt accaaatgaa ggcgaaatcg acaacacaat cgaagccatc cttgctaaag
     8821 tgccaagcaa gaaagtttgg atcaaccctg actgtggttt gaaaacacgt ggtatcccag
     8881 aaacaaaaga aagcttgatc cgccttgttg aagcagctaa agctgcgcgt gagaaattgt
     8941 aagattggat ttctctccat acagtggttg ttcaaaaaga aatcaactag aaaaggttat
     9001 tacatatgtc acgccaaaca ccgtcactct catttgaagt gttccctcca aacccagccg
     9061 tgggtaatgg taacattatt tcagccttgc aggatatgca ggagttggct ccccatttta
     9121 tcagtgtaac tgccagcaat aataaattta atatcaagga aacgacggtt cgtttggctg
     9181 actttatcca aaatgacttg gcgattccga ccattgccca cttgccagct atttatctaa
     9241 ctaaggacaa ggttgctgaa actattgctg acttggacaa agttggggtg cagaaaatct
     9301 tggctcttcg tggggatatt attccagacg tagaaccaca aaaggatttc cgctatgcaa
     9361 ccgacttgat tgagtttatc aaggaacaag cccctcactt tgatattatt ggagcttgct
     9421 atccagaagg gcatccagat tcgccaaatc agatttcaga tattcaaaat cttaagaaga
     9481 aagtggatgc aggctgttcg agcctcgtaa ctcagctctt ctttgataat gagcgcttct
     9541 atgattttca ggacaagtgt attttggctg ggattgatgt tcccattcat gcaggaatta
     9601 tgccaattct gaatcgaaat caggctctcc gactcttgaa gacttgtgag aatatccatc
     9661 ttccacgcaa atttaaagcc atcttagaca agtatgagca tgaccctgag tcgctcagag
     9721 cagcaggact tgcctatgca gtggaccaaa tcgtggactt ggtaactcag gatgttgccg
     9781 gtgtgcatct ctatacgatg aataatgctg atacagcaaa atacatccat caagcaaccc
     9841 atgccttgtt taatcaccag tctctaggat aataaaagca aaccattctt ctcaggtgag
     9901 gggaatggtt cctttttaat ggtaaagacc tcacttttta agaaaaatat gataaaataa
     9961 attctgtacg tacttgatac aaagatgaag gtattaaata atacaatgca tttaaactgt
    10021 aagatttaac gacacgagtg agtcaaatcg atgttatttg acgagagagt tagtaaagca
    10081 tttagctaat cgcccgatag cgattagcgt gaaagcttta gatactttag tattaaacct
    10141 ttctaatgat gtatcttgaa aactgaactt gcctacaact ctgtggaaaa agataaatct
    10201 ccctaggagc agtcgctccg tcgttcgatt tcctattttc ctttgagttg cttgacggct
    10261 tggtatcttg atgacttgaa ttttggtctc atttcttagg aaaaaagata agcttcctag
    10321 cactcgtcga gtgcgacgtc accttcctat ttttctacag aaatgttcgg caagccgaac
    10381 cgtccaaaat atcttgtgta attgaacacg gccgaaaagc tgtgtaaaaa agataaactg
    10441 tcttgtcttc atcgaagact tcgtcagttt cctattttta ctttgctttt gacgtccttg
    10501 gtatcttgat ctttgtaggc aaggcgtata atttcatcaa tccaaagggg attaaaatgg
    10561 caaaacaagt gtttcaaacg acttttgcgg gtcgtgagtt aattgtagaa actggtcagg
    10621 ttgctaagca agcaaatggc tctgttgtcg tacgttacgg tgagtcaact gtcttgactg
    10681 ctgccgttat gtctaagaaa atggcaactg gggatttctt cccactccaa gtcaactacg
    10741 aagaaaaaat gtatgcggct gggaagtttc ctggtggctt tatgaaacgt gaaggacgtc
    10801 cttcaacaga tgcgaccttg acagcgcgtt tgattgaccg tccgattcgt cctatgtttg
    10861 cggaaggttt ccgtaatgaa gtccaagtca tcaatacagt gctttcttat gatgaaaatg
    10921 cgtctgcacc aatggctgct atgtttggtt catctttggc actgtctatt tcagatattc
    10981 catttgacgg accaattgct ggggtacaag tgggatatgt agatggccaa atcatcatca
    11041 acccaagtca agaacaagca gagcaatctc ttcttgaatt gacagtagct ggaaccaagc
    11101 acgctatcaa catggtagag tctggtgcta aagaattgtc agaagaaatc atgttggaag
    11161 cgctccttaa agggcacgaa gctgtcaaag aattgattgc cttccaagaa gaaatcgttg
    11221 ctgctgtcgg taaagaaaaa gcagaagtgg aattgcttca cgtggatgct gaattgcaag
    11281 ctgaaatcat tgcagcctac aacagtgacc tccaaaaggc agttcaagta gaagagaaat
    11341 tggcccgtga agctgcgact caagtagtga aagaccaagt gactgccgtt tacgaagaaa
    11401 aatatgcgga ccacgaagaa tttgaccgta ttatgcgtga tgtggctgaa atcttggaac
    11461 aaatggaaca cgcagaagtg cgacgtttaa ttacagaaga caaggtgcgt cctgatggtc
    11521 gtaaggtcga tgaaatccgt cctttggatg cggttgtcga cttccttcct cgtgtgcacg
    11581 gttcaggtct cttcactcgt ggacaaacgc aagccctttc tgtcttgacc ttggctccga
    11641 tgggagaaac tcaaatcatt gatggtttgg atctggagta caagaaacgc tttatgcacc
    11701 actataactt ccctcaatat tctgtagggg aaacaggtcg ttacggtgcg ccaggtcgtc
    11761 gtgaaatcgg tcacggtgcc cttggtgagc gtgctcttgc tcaagtcttg ccaagcttgg
    11821 aagaattccc atacgctatc cgtctagtag cagaagtttt ggaatcaaac ggttcttcat
    11881 ctcaagcttc tatctgtgcg ggaactcttg cccttatggc tggtggtgtg ccaatcaagg
    11941 cgccagtagc tggtattgct atgggactta tctcagatgg aaataactac acagtattga
    12001 cagatatcca aggtttggaa gatcactttg gagatatgga cttcaaggtt gcaggtactc
    12061 gtgatgggat tacagccctt caaatggata tcaagattca agggattact gcagaaatct
    12121 tgacggaggc tcttgctcaa gccaagaaag cgcgttttga aatccttgat gtcattgaag
    12181 caaccattcc agaagttcgt ccagaattgg ctccaactgc tccgaaaatt gatacgatca
    12241 agattgatgt ggacaagatt aagattgtca tcggtaaggg tggagaaacc atcgacaaga
    12301 ttatcgctga aacaggtgtt aagattgata tagacgaaga aggaaatgtg tctatctact
    12361 ctagtgacca agatgctatt aaccgtgcca aagaaattat tgctggtttg gttcgtgaag
    12421 ccaaagtgga tgaagtttac cgtgctaaag tcgttcgtat cgagaaattt ggtgcctttg
    12481 ttaacctctt tgataagaca gatgcccttg ttcatatctc tgagatggct tggactcgta
    12541 ccaatcgtgt agaggatttg gtagaaatcg gggatgaagt tgatgttaag gttatcaaaa
    12601 ttgatgaaaa aggccgtatc gatgcctcta tgaaggctct tctacctcgt ccgccaaaac
    12661 ctgagcatga tgaaaaaggt gaaaagtctg agcgccctca ccgcccacgt catcaaaagg
    12721 atcacaaacc taagaaagaa tttacagaaa caccaaaaga ttcagaataa gaaaaggaga
    12781 aatgtatggg gtggtggcgc gaaaccattg atattgtaaa agaaaatgat ccagcggccc
    12841 gcaccacttt ggaggttttg ctgacttatc caggtgtcaa ggccttggcg gcccaccgtc
    12901 tctcgcattt tctctggaag cacggcttca aactattagc tcgtatgtac agtcagtttt
    12961 ggcgcttttg gactcagatt gagattcatc caggagccca gattgattca ggtgttttta
    13021 ttgaccatgg ttctggtctg gtgattggag agacagcgat tgttgaaaaa ggcgttcttc
    13081 tctatcacgg agtgactctc gggggaacag ggaaagactg tggcaaacgc catccgactg
    13141 tacgaaaggg agccctcata tcagcccatg cccaagttat cgggcctgtg gaaattggtg
    13201 aaaatgccaa ggtcggtgct gcagcagttg tcgtagcaga tgtacctagt gatgtgacgg
    13261 ttgtcggtat tccggccaag attgtccgtc ttcatggtaa gaaagatgag cctgttattc
    13321 acgaagtcga agaaaaacgg gagtattatg tcaataaact cgagcaggct aaagatgcca
    13381 gtcacagatc gtctggtttg tagaggatac ctatatgatt caagcaagaa acaagttaag
    13441 ccaagaggag ctatctgagg cgaaaaaagt aattaactgt tgccaaaact atgacggtac
    13501 ctatcgcgat ccctatctct ctaacatgct taattttgac ccaaacatgc ccgccttttt
    13561 cctttattat gaaaaaggca aacttgttgg tttattaact gtctatgcag atgaccaaga
    13621 tgtagaagtg acgatattgg ttcatccagg tcatcgccgt caggggattg cgcgtgcatt
    13681 gtttactagt tttgagagag aaacagcttc tttccccatt cggtcagtca cttttcagac
    13741 agaacgtatt tttttagaga atcatcctga ctttgccagt aactggggac taatcgagga
    13801 tgaagagaca gaaacttggt taggtaagga tagaagacct tatccgttag caaatgtttc
    13861 ccatcttgaa gttttgttag cagatagttc gtatcaggat caaattagtc aattaaaatt
    13921 tcaggcattt tcagaggaac atgaatcgag agaagttgtg gatagatatg tcgctgaagc
    13981 tctgaaggat ccagaaagtc gcttatatat tttgttaaaa gacggtcagg ttattgggac
    14041 ttgcacggta gatttatcga ctaatacgaa ttacttctac ggtttagcaa tatcggaacc
    14101 tgaacgtggg aaaggctatg gaagctactt agcaaaatcc ctcgtcaacc aactaattga
    14161 gcaaaatgac aaggaatttc agattgcagt ggaagatagc aatgtaggtg ccaaacgttt
    14221 gtatgaaaaa attggctttg tcaaacagac acaggtggtt tatctgaatg agaaaggagc
    14281 aagggattcc gaagtgtaga gacattcgga ctgaaattta cttgagctct tagtttagtg
    14341 atgaaactaa cagttcttgg attttagctt tcctgattat gatttatgat taaaatctat
    14401 gacaccatgt ctcgtgattt gcgagaattt gtcccgattg aggacggcaa gatcaagatg
    14461 tatgtttgtg ggccaaccgt gtacaactat attcacgtgg gaaacgcccg ttcgacggta
    14521 gcttttgata cgattcgtcg ctattttgag taccgtgggt ataaggttgc ctatatttcc
    14581 aattttacag atgtggatga taagattatc aaccgtgcca gggaagaagg catcacacct
    14641 caggaggttg cggataagta catcgctgcc tttcgtgagg atgtgacggc cttaggcgtg
    14701 aaacctgcga ctcgccatcc gcgtgtagtg gagtttatgg cagacatcat ccgttttgtg
    14761 gaagacttga tcgagaaagg ctttgcctat gagagtcaag gggatgtcta tttccgtgta
    14821 gaaaaatccc acaactatgc taaattggct aataaaacct tggaagattt ggagctaggt
    14881 gcttcaggtc gtaccgatga agaaacggct cgtaaggaaa atcctgtaga ctttgctcta
    14941 tggaaatctt ccaaaccagg tgagatttct tgggatagtc cttggggacc tggacgtccg
    15001 ggttggcata ttgagtgttc agtcatgtcg acagagattt taggtgatac cattgatatc
    15061 cacggtggtg gagctgacct agagtttcca caccacacca atgaaattgc ccaatctgaa
    15121 gctaaaacag gcaaggcttt tgctaactac tggatgcaca atggctttgt caatatcgac
    15181 aatgtcaaaa tgtctaagtc cttgggtaac ttcattacgg tacacgatgc ccttaaaact
    15241 cttgatgggc aagtacttcg tttcttcttt gcgacccagc actaccgtaa gcctatcaac
    15301 ttcacggaaa aggcagttcg cgatgccgag accaatctca agtatctgaa gaatacttac
    15361 gaacaaccat ttacaggaaa cgtggatgcc caagagttac aaaactttaa agataagttt
    15421 gtagcagcta tggatgaaga ttttaatgct gccaacggta tcacagttgt ctttgaaatg
    15481 gccaaatgga tcaactcagg gaactatgat gcaagtgtca agcaagctct tgcagatatg
    15541 ttagaaattt ttggaattgt ctttgttgag gaagttttgg atgcagagat tgaagacttg
    15601 attcaaaaac gccaagaggc gcgtgccaat cgtgactttg agacagcaga ccaaatccgt
    15661 gaccaattgg ttactcaagg aattaagctc cttgatacca aggatggagt gaggtggaca
    15721 cgtgattgat gtcaatctca ttaacgggat tgcgctagcc tttgagggag atgcggtgta
    15781 ttctatgtat attcgccgcc acctcatcct caaaggtatg accaagccca ataaactcca
    15841 ccaagaggcg accaagtacg tgtcagccaa ggctcaggct cgtctgattg ctctcatgtt
    15901 ggaggagcag atcctaacgg aaaaagaaga agaaatctac aaacgtggcc gcaataccaa
    15961 tagtcacaca aaggctaaaa atgcagatgt cgtgacttat cgtatgtcca cgggatttga
    16021 agcagttatg ggctatctcc atatgactga gaatctggaa cgtcttgaga gtttggtttc
    16081 atggtgcatc caaaaagtgg agggctagaa catgagggca aaagaactac aagactggtt
    16141 tcctgaggct cggatttcag accaaccagt agagaaagag ggctatctca cgcttccttt
    16201 agcttctcag cagtggattt tgctggagga agctgggctc agcgagcgtg aaaagcagtt
    16261 ggttgccctt ttgacccagc aggagcaggc tcgttcgcta aacccttggt attcctatct
    16321 ggttgagggc aagggacagg caccgcaagt ttttaaaaag attcagttgg tttattgcca
    16381 tctttcttat tttcagcagg aaaatctgac ttcttggcta gatatgatgc ggactctttt
    16441 tccgaattgt cagacagtgc tacaggtcgg agctcaggat tatgttttcg tgcttcaaca
    16501 agacaaatac acttctgtaa gagatatttt gagtgatacg attgaagcgg ttgagtatga
    16561 ctttggactt cgtctttcta tcatgttggg tcaggtttgg tctcagacag gacatcaagc
    16621 cctatcagac ttaatcaaag ctgagcggga tttgttcaag acatggtggc gtcagggtca
    16681 ccaaggtgtt catacttttt ctcagctcta tctttggagt atgggagaaa gactcgtgga
    16741 cttgaagcca attaaagaat gtctacacca gatgattttg gatcaagatc agattcagga
    16801 aatcattctc tctctttggg aaaatagtgc tgttctcact aaaacagccc agcaactcta
    16861 tctgcaccgc aattctctcc aatacaagat tgataaatgg gaagagttga cagggcttca
    16921 gttgaaagag ttgaccgacc tgaccttgtg ttatcaattg attttaggtt ctttgtcaac
    16981 tatagttggt ttgtaaagaa gttaata
//
LOCUS       DAVKFH010000035        15354 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKFH010000035 DAVKFH010000000
VERSION     DAVKFH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.035"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            82..879
                     /locus_tag="VW006_001354"
     CDS             82..879
                     /locus_tag="VW006_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9380199.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1027..1149
                     /locus_tag="VW006_001355"
     CDS             1027..1149
                     /locus_tag="VW006_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU9380200.1"
                     /translation="MTCDFKSETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1171..2211
                     /locus_tag="VW006_001356"
     CDS             1171..2211
                     /locus_tag="VW006_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU9380201.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWMASGKFPQFVDEGSSCHNLSYTRDVGAVAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDHLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2382..3656
                     /locus_tag="VW006_001357"
     CDS             2382..3656
                     /locus_tag="VW006_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9380202.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELCLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3666..4544
                     /gene="truB"
                     /locus_tag="VW006_001358"
     CDS             3666..4544
                     /gene="truB"
                     /locus_tag="VW006_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9380203.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4785..5087
                     /locus_tag="VW006_001359"
     CDS             4785..5087
                     /locus_tag="VW006_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9380204.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5195..5833
                     /gene="udk"
                     /locus_tag="VW006_001360"
     CDS             5195..5833
                     /gene="udk"
                     /locus_tag="VW006_001360"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9380205.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5961..7301
                     /locus_tag="VW006_001361"
     CDS             5961..7301
                     /locus_tag="VW006_001361"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9380206.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7279..7491
                     /locus_tag="VW006_001362"
     CDS             7279..7491
                     /locus_tag="VW006_001362"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9380207.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7488..8363
                     /locus_tag="VW006_001363"
     CDS             7488..8363
                     /locus_tag="VW006_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9380208.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8356..9171
                     /locus_tag="VW006_001364"
     CDS             8356..9171
                     /locus_tag="VW006_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9380209.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            9164..9595
                     /locus_tag="VW006_001365"
     CDS             9164..9595
                     /locus_tag="VW006_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9380210.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9602..11269
                     /gene="recN"
                     /locus_tag="VW006_001366"
     CDS             9602..11269
                     /gene="recN"
                     /locus_tag="VW006_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9380211.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11271..11999
                     /locus_tag="VW006_001367"
     CDS             11271..11999
                     /locus_tag="VW006_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9380212.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12078..13901
                     /gene="lepA"
                     /locus_tag="VW006_001368"
     CDS             12078..13901
                     /gene="lepA"
                     /locus_tag="VW006_001368"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9380213.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14195..14275)
                     /locus_tag="VW006_001369"
                     /pseudo
     CDS             complement(14195..14275)
                     /locus_tag="VW006_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14449..14924
                     /locus_tag="VW006_001370"
                     /pseudo
     CDS             14449..14924
                     /locus_tag="VW006_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            14962..15264
                     /locus_tag="VW006_001371"
     CDS             14962..15264
                     /locus_tag="VW006_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9380214.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEARPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            15272..>15354
                     /locus_tag="VW006_001372"
     CDS             15272..>15354
                     /locus_tag="VW006_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEU9380215.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEE"
BASE COUNT         4583 a         2782 c         3594 g         4395 t
ORIGIN      
        1 cttttatgaa atacagaaaa cgcttcctaa aacctaaagt ttgtgatata attatcaaga
       61 aaaaagattt aggagtttat tatggtttct tcagaattta tctcaaagat tgaatttgct
      121 tgcaataaga aagaaagtct ttatagtcaa agcaaattta agtatgcgat tcgttcgatg
      181 ttcgcaggtg catttttaac cttcagtact gctgcaggtg cagttggggc tgacttgatt
      241 aataaaattg caccaggtag tggacgcttc ctctttccat tcgtttttgc ttggggcttg
      301 gcctacattg tttttttgaa tgccgagttg gtcacttcaa acatgatgtt cttgactgct
      361 ggtagtttct taaaaaaaat ctcttggaga aaaacagctg agattttact atactgtacc
      421 ttgttcaacc ttatcggagc cttgatagca gggtggggct ttgctcattc ggcagcctat
      481 gcgaatctga tacacgatag tttcatctca ggtgttgttg agatgaagtt aggccgctca
      541 aatgaattgg tcttgcttga ggcgattttg gcaaatattt tcgtaaatat tgcgattctg
      601 tcatttattt tggtcaaaga tggtggtgcc aaactttggc ttgtgttgtc agctatttac
      661 atgtttgtat tcttaacaaa cgagcacatt gcggcgaact ttgcttcttt cgcgattgtg
      721 aaattcagtg ttgctgcgga ttcaattgcc aacttcggtg ttggaaatat gcttcgccac
      781 tggggtgtga ctttcatcgg aaactttatc ggaggaggcc tcttgatggg tcttccatat
      841 gccttcctca ataaaaacga agatacttat gtagattaag aaaatgagca cgattgagtc
      901 gtgctttttt cattttcaaa ataaggtaat agctatttct tatatcaaaa tatagaaaac
      961 tgatatttgt aaactataac tcaaggtgct acaatatcct taataaaatg atatggaggt
     1021 caccttatga cttgtgattt taaatctgaa actctacaac tacatgctgg tcaagttgtg
     1081 gctccagcta ctaagtctcg tgcagtgccg atttatcaaa caacattttt tgtttttgat
     1141 gacacgtagg aaggtgccga tctgtttgcc ttgaggaaac cagggaacat ttatactcgt
     1201 atcaccaatc ctacaacagc tgcccttgaa ggtggtgttg aagcgctagc aacagcatca
     1261 ggtatgactg cagtgactta tacgattttg gcgcttgccc atgctggtga ccatgtagtg
     1321 gctgcttcga ctatttacgg tggaaccttc aatcttttga aagaacccct tcctcgttat
     1381 ggtatcacaa caacctttgt cgatgttgat aatttggagg aagtagaagc agctatcaaa
     1441 gacaatacca agcttgtctt gattgaaacc ttgggtaact ccttgattaa tattccagac
     1501 ctggaaaaac tggcagagat tgctcataaa catcaaatcc cacttgtgtc agacaatact
     1561 tttgcaatac cttatttgat taacgtcttc tctcatggcg ttgacattgc cattcactct
     1621 gcgactaagt ttatcggtgg gcatggtaca actattggag gaataattgt cgatagtggt
     1681 cgttttgact ggatggcttc agggaaattc cctcaatttg ttgacgaggg ttcaagctgc
     1741 cacaatttga gctatactcg tgatgtgggt gcagtagcct ttattatagc tgttcgagtt
     1801 caattgcttc gtgatacagg tgcagccttg tcaccattca atgctttcct cttgctacaa
     1861 agacttgaaa cctcttcact tcgtgtggaa cgccatgtac aaaatgctga gacaattgtt
     1921 gattttcttg tcaaccatcc taaggtagaa aaggtaaatt atccaaaact tgcagatagt
     1981 ccttatcatg ccttggctga gaaatacttg ccaaaagatg tcggttcaat ctttaccttc
     2041 catgtcaaag gtggcgagga agaagcacgc aaggtcattg atcatttaga aatcttttct
     2101 gaccttgcaa acgcggcaga tgctaaatcg cttgttgtcc atccagcaac aaccactcac
     2161 ggtcaattgt cagaaaaaga cctagaagca gcaggtgtca caccaaacta aattcgtttg
     2221 tcaatcggtc ttgaaaatgt agaagatttg attgaagact tgcgcttggc cttggaaaaa
     2281 atttaaagta aaagaagata aacagtgggc ttcgactcac tgtttttgat tttccctcag
     2341 gcatgatata atggttaaag aagtctagaa agaggaacga tatgaacgaa atcaaatgtc
     2401 ccaactgtgg ggaagtcttt acagtaaatg agagtcagta tgccgaactc ttgtcccaag
     2461 tgagaacggc agagtttgat aaggaactac acgataggat gaagcaggaa ctggccttgg
     2521 ctgagcaaaa ggccatgaat gagcaacaga ctaaactggc tcagaaggat caagaaattg
     2581 cgcaattaca gagtcagatc caaaactttg atacagaaaa agaattggcc aagaaagagg
     2641 ttgaacagac aagccatgag gctctcttgg ctaaggacaa ggaagtacag ctcttagaaa
     2701 atcagttggc taccttgcgt ttggagcatg aaaatcaact gcaaaagacc ctttctgacc
     2761 tagaaaaaga acgggatcag gttaaaaacc aactactttt gcaggaaaag gaaaatgaat
     2821 tatgtttggc ttctgttaag caaaactacg aagcccagct caaggcagct agtgaacaag
     2881 tcgagtttta taaggatttt aaggctcaac aatctacaaa agcgattggg gaaagcctag
     2941 aacagtatgc agagagtgag tttaacaagg ttcgtagttt cgcctttcca aatgcttact
     3001 ttgagaagga taacaaggtc tcttcgcgtg ggtctaaagg ggactttatc ttccgtgagt
     3061 gtgatgaaaa tggagttgaa atcatttcta tcatgtttga gatgaaaaac gaagcagacg
     3121 gaacagagaa gaagcacaag aatgcagatt tttacaagga attggacaag gaccgtcggg
     3181 agaagaactg tgagtatgcc gttttggtga ccatgcttga ggccgataat gactacttta
     3241 acacagggat tgttgacgtc agccacgagt atgaaaaaat gtatgttgtt cgtcctcaat
     3301 tctttatcca gttgattggt ctcttacgta atgcggcgct aaattcccta aaatacaagc
     3361 aggagttggc cttggttcgc gagcaaaata ttgacattac gcattttgag gaagacttgg
     3421 atgcctttaa gctagctttt gctaagaact ataattcagc ttcgactaac tttggaaaag
     3481 ctattgatga aatcgacaag gccatcaaac gcatggaaga ggttaagaaa ttcctgacta
     3541 catctgaaaa ccaactccgt ctagctaaca acaaattgga agatgtctct gttaaaaaat
     3601 tgacccggaa aaatccaaca atgaaagcga agttcgaagc actgaagggg gagtagaaag
     3661 caaaaatgaa cggtattatc aacttaaaaa aagaagcagg aatgacctcg catgatgcgg
     3721 tttttaaact gcgtaagatt ttgggaacca agaaaattgg tcatggtgga accttggatc
     3781 cggatgtggt gggtgttttg ccgattgcgg ttggcaaggc gacacgcatg gtcgagttta
     3841 tgcaggacga gggtaagatc tatgaggggg aaattactct gggttattcc acgacgactg
     3901 aggatgctag tggggaagtg atcgcagaaa cccctgtttt gtctcccttg gatgaaaagc
     3961 ttgttgatga agcgattgct agcttgactg ggcctattac ccagattccc cctatgtatt
     4021 cggcagttaa ggttaatggt cgcaagctct atgagtatgc gcgtgctggt caggaagtgg
     4081 agcgtccaga acgtcaggtg atcatttatc aatttgagcg aacaagtccg atttcttatg
     4141 atggccaact tgcccgattc acttttcgtg taaaatgcag taaagggact tacatccgta
     4201 ctttgtcagt tgatttgggt gaaaagcttg gttatgcggc tcatatgtcc catttgactc
     4261 gtactagtgc tgctggctta caattagaag acgctctttc cttggaggaa attgctgaaa
     4321 aagtagaggc tgggcaatta gattttctcc atcctttaga gattgggaca ggtgaccttg
     4381 tcaaagtttt cctaactcca gaagaggcta cagaagttcg ctttggtcgt tttattgagc
     4441 tagaccaaac ggacaaagaa ctggctgcct ttgaagatga taaattgtta gccattctag
     4501 aaaaacgggg caatctctat aagccaagga aggtttttag ctagatcgtt taggaataaa
     4561 aatcgggtga tagataacaa ttgcttgata aaaccccata ctaatagtag aatggttttg
     4621 ggaattataa tattccaatt gttgcgagtt gtaggtactc aaataatcta tatagaaatt
     4681 tagaggtgtg aaatgaagca atttaaaatt ctttcagata aatatttaga gtccattaca
     4741 ggttctggtg ggaacttagg cccaggattt ggtgtgataa ttccatgatg cgaaatgagt
     4801 ttcgagaaag ggtggagcaa cttcttcaac aaaaagaaat aaatgaaaat agtgagttga
     4861 gtcacctgtt tcgtcttgct atacaaaatt tagacagaaa tgaaaaatac caatcggtca
     4921 tggccaattt gagtcaaggg ttgtcacttt acctcatgac gcatcattac caggcaccta
     4981 agtctgtcat tgattttggt ttatggattg ccaaagctcc tagccaggaa agagggagac
     5041 tggctttctt gcagatgctt gctcaaaccc tacagggctt tcgttaaaac tgtcgaacat
     5101 cagagggaag actctggtgt tttttgctga tgagactata aattttgtaa aaaatgtgat
     5161 agaatagacg acggataaaa taacggagga tagcatgcaa aatagaccaa tcattattgg
     5221 agtgacaggt ggttctggtg gtggtaaaac cagtgtttca agagccattt tatcgcattt
     5281 tcctgatgaa aagatttcca tgattgagca tgattcatac tacaaggatc agtctcattt
     5341 gacctttgaa gagcgtgtca aaaccaacta tgaccatcct tttgcctttg atacagactt
     5401 gatgatcgag cagattaagg aattgttggc agggcgtccg gtggacatcc cgacctacga
     5461 ctatacagag catacacgga gtagcaagac ctatcgtcag gagcctcaag atgtctttat
     5521 cgttgagggc attttggtct tggaggacaa gcgtctgcgc gatttgatgg atatcaagat
     5581 ttttgtggat acggatgacg atgtgcgtat tattcgtcgt atcaagcgtg atatggagga
     5641 acgtggccgt agccttgata gcgttattaa ccagtactta ggtgtggtca aaccaatgta
     5701 ccaccagttt atcgagtcaa ctaagcgtta tgctgatatc gtcattcctg aaggggttag
     5761 caataccgtg gctatcgacc tgttgacgac caagattgca aagattttgg aagaagctcg
     5821 aaacagcaaa taatcacatg tggaggcctt gcctcctttt tctatttttc cctcataatg
     5881 gtacataaat ggagattttt aggcatattt ttggtataat aatacccata aaagagcaag
     5941 taaatgaata gtaggtggag atggaaaagt atttatcggt aacaactttg accaaatatc
     6001 tgaaaatgaa attcgataaa gacccatact tggaacgggt ctatttaact ggtcaagttt
     6061 ccaactttcg taaacgacct actcaccaat atttctccct aaaggatgac catgcagtta
     6121 ttcaagcgac catctggtct gggatttatc agaaattagg gtttgacctg gaagaaggaa
     6181 tgaagatcaa tgtgattggg cgtgtacagg tctatgaacc aagtggtagc tactccatca
     6241 tcattgaaaa agctgagcct gatggggttg gggcgcttgc gattcagttt gaacaactta
     6301 agaaaaaatt gacagaagaa ggcctgtttc aagaacgctt caagcaagct ctaccccaat
     6361 tttctaagag aattggggta gtaaccagcc gtagtggagc cgttattcga gatattatca
     6421 cgaccgtcag caggcgattc ccaggtgttg acatccttct ttatcctacc aaggttcaag
     6481 gtgaaggggc tgcggaggaa attgctagaa atattgcgcg tgctaatcaa cgggacgatt
     6541 tggatttgct cattattggt cgtggaggtg gttctattga ggatctctgg gcctttaacg
     6601 aagaaattgt ggtacgagct atttttgaat ctcgtttgcc agttatctct agtgtggggc
     6661 atgagacgga tgtgaccttg gcagattttg tggcagatcg acgcgctgca acgccaacag
     6721 cggcggctga actggcaaca cctgtgacca agttggatgt attaactcat ttgcaaaatc
     6781 aggaaaaacg gatggcaacg gcagtccgaa atgttctatc taagaaacaa gaggctttga
     6841 aaaaatgcag tcagtctgtt atctttagac aacctgagcg cttgtatgac ggttatttgc
     6901 aacgcttgga ccaactgcaa ctgcgtttga aacaaagttt gcgaactcgg atttctgata
     6961 acaaacaatt agttcaagca agaactcatc aattagtaca attatcacct gttaccaaaa
     7021 tccaacgcta tcaagaccgt ttaggacagt tggacaagct cttaggtagc caaatggcgt
     7081 tagtttatga cgccaaggtt gctgaggcca agcgactttc ggaagctttg ctcatgttgg
     7141 atactagccg aatcgtggcg cgtggttatg ctattgtcaa aaaagaagag tccattgtag
     7201 attcggttga gagtttgaag aaaaaagacc aagtaacgct tttgatgcga gatggtcaag
     7261 tagaattaga ggttaaagat gtcaaaacaa aagaaatttg aggaaaatct agcagaactg
     7321 gaaaccattg tccaaagttt ggaaaatggt gaaattgctc tggaagatgc gattactgcc
     7381 tttcaaaagg gcatggtctt gtcaaaagag ctccaagcta cgctggacaa ggctgaaaag
     7441 accttggtca aggtcatgca agaagacgga acagaaagtg attttgaatg aaaaagcaag
     7501 aaaaattagc tcttgtcgag tcggctttgg aagattttta tggagaccag cagtttgcct
     7561 ctagtttacg ggagtctgtt ctctattcta ttcatgctgg tggcaagcgt attcggcctt
     7621 ttctcttgtt agaagttctg gaagccttgc aggttaccat caaacctgct cacgcgcagg
     7681 tagctactgc cttggagatg attcatacag ggagcttgat tcacgatgac cttcctgcta
     7741 tggatgatga cgattatcga agagggcggt taaccaatca caagaaattc ggtgaagcta
     7801 tggccatttt ggctggagat gccttattct tagacccata tgccttgatt gcgcaggcag
     7861 atttgccaag tcagattaag gtggacttga ttgccaactt atcccttgct tcaggtagtc
     7921 tgggtatggt ggcagggcaa gttttggata tggagggcga acaccagcac ttgtctttgg
     7981 aagaacttca gattattcat gccaataaga ctgggaagtt actagcctat cccttccaag
     8041 cggcagctat tatagctgaa ttgtcacctg aaatgcaggt gaagctgaaa actgtgggtg
     8101 aattgattgg acttgctttt caagtcagag atgatgtact ggatgtgaca gctagttttg
     8161 aggaaatcgg caagacacct caaaaggatc tgcaggcaga aaaatcaacc tatcctgccc
     8221 tgttgggctt ggaagagtcc attgcctttt gtaaccaaac cctggatcaa gctaatgaaa
     8281 aattagaaga aattgcccag cagcttccct ttgaaacaga atcgattgta agtgtagtag
     8341 aaagtttgag aatcaatggc taaggaaaga gtggatgtac tagcttataa acaggggttg
     8401 tttgaaacga gagagcaggc caagcgaggt gtaatggctg gcctagtcgt agcagtcctt
     8461 aatggagaac ggtttgacaa gccaggagag aaaattccag atgacaccga attaaaactc
     8521 aagggagaga aactcaagta tgtcagccgt ggtggtttga aactggaaaa ggccttgcag
     8581 gtctttgatt tgtcggtgga tggcgcgact acgattgata tcggggcctc tactggaggt
     8641 tttaccgatg tcatgctaca gaatagtgcc aagttggtct ttgcagtcga tgttggtacc
     8701 aatcagttgg cttggaaatt acgccaagac ccacgagttg ttagcatgga gcagttcaat
     8761 ttccgctatg ctgaaaagac tgatttcgag caggagccga gctttgccag tattgatgtg
     8821 agtttcattt cccttagtct gattttgcca gccttgcatc gtgtcttggc tgatcaaggt
     8881 caggtggtag cacttgtcaa acctcagttt gaggcaggac gtgagcagat tgggaaaaat
     8941 ggaattattc gagatgctaa gattcatcag aatgtccttg aatctgtaac agctatggca
     9001 gtagaggcag gtttttcagt ccttggcttg gacttttctc ccatccaagg tggacatgga
     9061 aatattgaat ttttagcgta tttgaaaaaa gaaaagtcag caagcaatca gatttttgct
     9121 gagattaaag aagcagtaga gagggcgcat agtcaattta aaaatgaata aaaaagagag
     9181 acttgaaaaa attagaagat tggtgacaga ttatcaaatc ggcacgcaag aagaaattgt
     9241 agaacatttg aaagaagcag gtatcactgc cactcaggcg acggtatccc gagatatcaa
     9301 agagttaggt attgtcaaaa ttcctttgag agacaacacc tatgtctacg aattaccaaa
     9361 atcaatcgta aaaagtctgc aactggctga agacaatatc gaatcggctg aattgatgga
     9421 taagatgatc aatctccaag ttattccagg aaatacggct tttgtaaaag ctcagttaat
     9481 cgatactttt gcagacaaga tttttagttg tttgactgat gatagctcga ttttagtcat
     9541 tgccagaagt ggaagtctag cagaggaaat ctttgaacaa gtaaaaaatt ggtaggtcta
     9601 tatgttactt gaaatttcga taaaaaactt tgccattatt gaggctattt ccctcaattt
     9661 tgaaaagggg atgactgtcc tgactggtga aacgggtgca gggaagtcga ttatcattga
     9721 tgccatgaat atgatgttgg gagctcgtgc gacgacagat gttattcgtc atggtgcacc
     9781 aaaggcagag attgaggggc ttttctcagt tgagaatagt cgtcttttac aggaaatttt
     9841 tgatgagcaa ggtttagaat taggtgatga aattatcatc cgtcgggaaa tcttgcaaaa
     9901 tggtcgtagt atcagccgtg taaatggtca gatggttaat ctgtctgttt tgcgagctat
     9961 tgggcaacat cttgtagata ttcatggtca gcatgaccat gaggagttaa tgcgtcccca
    10021 actgcatatc cagatgttgg atgaatttgg tgatgccgct ttttgggact tgaaagaaac
    10081 ctatcaaacg agttttgatg cctatcggaa aatgcgcaag caggttctgg aagtcaagaa
    10141 aaaccaacag gaacacaagg aacgtatcga aatgttggaa tttcaaatgg cagagattga
    10201 ggcagcaaac ttgcaggctg gagaagactt ggctctcaat caagagcgag ataaactcct
    10261 caaccacaaa aatattgcgg atacactgac caatgcctac agtatgttgg acaatgaaga
    10321 tttttcaagt ctggccaatg ttcgttcagc tatgaatgac atggaaagtg tcgaagagta
    10381 tgatcctgaa taccgtgaaa tttcaagctc tctgtctgag acctactatg ttttagaaga
    10441 cattagcaaa cgtttggaag ctatcattga ggaccttgat tttgatggca atcgcctgat
    10501 gcaggttgag aatcgtttgg acctccttca tactattact cgtaagtatg gtgggactgt
    10561 tgatgatgtt ttgctttatt ttgccaagat tacggaagaa tacaatctct tgacaggcaa
    10621 taatctttcg tctgaggaca tggaagcaga gcttaagaag ttggaagtca atcttgtcaa
    10681 tttggcaggt caacttgctt ctgctcgtca taatttggct cagcaactcg aagctgagat
    10741 taaacaagaa ctgcaagatc tttatatgga aaaagcccag tttcaggttc attttagtaa
    10801 gggaaaattc agtcgtgggg gaaatgaaat ggttgagttt tatatttcaa ccaaccctgg
    10861 agaagacttt aaacccttgg ttaaggttgc ttctggaggg gaattatctc gtctcatgtt
    10921 agccattaag tctgcctttt cacgtaaaga aggcaagact agcattgtct ttgatgaggt
    10981 ggatacggga gtttcaggtc gtgttgctca agctattgct cagaaaattc ataaaattgg
    11041 tcagcatggt caggttttgg ctatctccca tttgccacaa gtaattgcga ttgcagatta
    11101 tcaattcttt attgagaaga ttagtaatga ccattcaacg gtttcgactg ttcgtctctt
    11161 gacagtcgaa gagcgagtgg aggaagttgc caagatgttg gcaggtgatg atgtgacaga
    11221 agcagccctg acgcaagcca gagaattgtt gagaaacagg gagaaataag atgacagatt
    11281 attatgtaat tggagatgtt cacggaaaag ctgggatgct ggaagacctt cttaaaacct
    11341 gggatggtca gacccagttg ctctttctag gtgatttgat tgacagaggt gaggatagtc
    11401 accgtgttct tgaaatggtc aaggacttgg tggacaatca aggggctatc tgtttgtctg
    11461 gaaaccacga gtatatgttt ttgacttggc tagatgaccc agaagaaagt tatgaccatt
    11521 atcgtcgcaa tggtggagat acaaccatta actctatcct aggtcgtccc ttggatgcac
    11581 cagttgatgg agtagaagat gccaagcggg ttgccgctga agcgtcagac ttggtcgaat
    11641 tcattcgtca aatgccattt gtagtagaga cagacaagta tatatttgtt cacgcaggta
    11701 ttgatttgac tttggacgac tggcatgaaa caacagatta taaaaaagta tggcttagaa
    11761 aaccattcca cggagctgaa aatcatactg gaaaaaccat tgtcttcggt catacaccgg
    11821 tctatggttt gttacagcaa gaacgcggta catctgagct ttggactaca gaagatggca
    11881 agattggaat ggatggagga gctgtttatg gtggtgtcct tcacgggatt gtctttacag
    11941 accaaggaat gacagaacac cactttatcg aaaatgacgg ctttgtcgct gaagattagt
    12001 actcctagca gggtatggtc ttgtcaaaat atcaaaaaca atttataata aatagatacc
    12061 ctgaaaggaa gagaatcatg aacttagaag aattgaaaaa acgacagggg aagatccgta
    12121 acttctctat tatcgcccat attgaccacg ggaaatcaac tctagcagac cgcattttgg
    12181 aaaaaacaga gacggtttca agtcgtgaaa tgcaggccca gcttttggat agcatggatc
    12241 tagagcggga acgtggaatt actattaagt tgaatgccat cgagttgaat tacactgcaa
    12301 aagatgggga aacttatatt ttccacttga ttgacacacc agggcacgtt gactttacct
    12361 atgaagtttc acgttcgcta gctgcctgtg agggtgctat tttggtggtc gatgcggctc
    12421 aaggaattga ggctcaaact cttgccaacg tttatctggc tttggataat gatttggaaa
    12481 tcatgcctat cattaataaa attgacctgc cggctgcaga tccggagagc gtgcgtacag
    12541 agattgaaga tgtgattggt ttggatgcca gtgaagcagt tttggcttct gccaaggctg
    12601 gtattgggat tgaagaaatc ctcgagcaaa ttgtagaaaa agtaccagca ccaacgggtg
    12661 atgtgacggc gccacttaag gccttgattt tcgactctgt ttacgatgct taccgtgggg
    12721 ttatcctcca agtgcgtgtc atggacggag tggtcaaacc tggtgataag attcagctca
    12781 tgagcaatag taagaccttt gatgtggccg aagtcggtat ttttacacca aaagcggttg
    12841 gtcgtgattt ccttgcgact ggtgatgttg gttacattgc ggcgtctatc aagacggttc
    12901 aggatactcg tgtgggtgat accgttacct tggcaaccaa tcctgcggca gaaccattac
    12961 atggttataa gcagatgaat cctatggtct ttgcgggtct ctacccaatc gaatcaaaca
    13021 agtacaatga cctgcgtgaa gcgcttgaaa aattgcaact gaatgatgct agtcttcagt
    13081 ttgaaccaga aacatctcag gcacttggat ttggtttccg ttgtggattt cttggacttc
    13141 tccatatgga tgttatccag gaacgtttag agcgtgagtt caatattgat ctcatcatga
    13201 cagctccgtc tgttatttac aaagttaatt tgaccgacgg tgagtctatg gatgtgtcta
    13261 acccatctga gtttccagac ccaactaaga ttgcgaccat tgaagagcct tatgtcaagg
    13321 cgcaaatcat ggtaccacag gagttcgtcg gagcagtaat ggagctagct cagcgtaagc
    13381 gtggggactt tgtgactatg gactatattg atgataaccg tgtcaatgtt atctatcaaa
    13441 ttcctcttgc tgaaatcgtc tttgacttct ttgataaact taagtcttcg acacgtggtt
    13501 atgcaagttt tgactacgaa ttgtcagaat atcgcccatc taagctagtg aaaatggata
    13561 tccttctcaa cggagacaag gtggatgccc tcagctttat cgttcataag gactttgctt
    13621 atgaacgtgg gaaactcatc gttgataagc tcaagaaaat catccctcgc caacaatttg
    13681 aggttccaat ccaagcggct attggacaca agattgtcgc tcgtactgat atcaaggccc
    13741 ttcgtaagaa cgtacttgct aaatgttatg gtggtgacgt ttctcgtaag cgtaaactcc
    13801 ttgaaaaaca aaaagctggt aagaaacgca tgaaatccat cggatcagtt gaagttccgc
    13861 aagaagcctt cctcagcgtc ttgagtatgg atgaagaata agttatcaga aatgcctttc
    13921 cttgttcaca ggaaatttat ataagaatca atagtaaagc aggcttagat ggtctgcttt
    13981 tttagttttt atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtatg
    14041 tatggttact gacttcgtca gtttcatcta caatctcaaa accatgtttt gagctgactt
    14101 cgtcagtttc atctacaacc tcaaaaccat gttttgagct gacttcgtca gtttcatcta
    14161 caacctcaaa accatgtttt gagctgactt cgtcagtttc atctacaacc tcaaaactgt
    14221 gttttgagta acctgcggct agcttcctag tttgctcttt gattttcatt gagtattaga
    14281 acagaaaaat gcttggagta tttgtttgtg tgtttatttt tatataacaa actataaaca
    14341 caacaaaaat ataaaaaaag agacaaaaaa gaacagaaag taattaacaa cgatttcata
    14401 tagtgttata cttatagcat aaagttaatg gaaagaaggg aaaaccttat ggtattagat
    14461 tatttctttg acaaaaacct tgtgttttgc ttagaagcgg ataatcaaga ataactcttt
    14521 gatcaggtag caagcttgtt ggaggaacga gaaattgtaa ctccaactta tcgtgaagct
    14581 ttgatcacgc gtgaaaagtc atttccaact ggtttagata tggaatttct aggaaaggac
    14641 ttgcaaatgt aatgattcct cacacagata ttgttcataa tctagctgag aaagtggtgg
    14701 ttgttcgatt agagaaaccg gtgactttcc ataatatgat tgcgccaggt aaggaagtag
    14761 aagtatcctt gctcttcttt atcatcaata attcaagttc aagtcaaacg aacattcttg
    14821 ctcagttgat ggactttttc acaggaaatg gacatcttga agacctatca aaaatttccg
    14881 aaccagaaaa actttatgct tatatagatg aagcaatcgc ttaatcttgt ctattaaaaa
    14941 ataaaatcgg aggaagtcta tatgattaaa attcttgctg cctgcggtgc aggtgttaac
    15001 tcaagtcacc aaattaaaag tgctctagaa gaagaacttt caaaccgtgg ttacgatgtt
    15061 cactgtgatg cagttatggt gaaagatgta aacgaagacc ttatgaaagg ttacgatatc
    15121 tttacaccaa tcgctgcaac agaccttggt tttgaaccag gtatcccagt tatcgaagct
    15181 aggccaatct tattccgtat cccagcaatg agcgttccag tatttgacaa tattagactt
    15241 cctgcgaaac aaaatatggt atagtagttc tatgaatgat gaagcaagta aacaactaac
    15301 tgatgcacga tttaagcgtc ttgttggtgt tcagcgtacc acttttgaag agat
//
LOCUS       DAVKFH010000036        15182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKFH010000036 DAVKFH010000000
VERSION     DAVKFH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.036"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..267)
                     /locus_tag="VW006_001373"
     CDS             complement(<1..267)
                     /locus_tag="VW006_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9380216.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVV"
     gene            complement(264..806)
                     /locus_tag="VW006_001374"
     CDS             complement(264..806)
                     /locus_tag="VW006_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9380217.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLVKSRYSEEVVA"
     gene            complement(819..2312)
                     /locus_tag="VW006_001375"
     CDS             complement(819..2312)
                     /locus_tag="VW006_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9380218.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            complement(2820..3986)
                     /locus_tag="VW006_001376"
     CDS             complement(2820..3986)
                     /locus_tag="VW006_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9380219.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(3999..4823)
                     /locus_tag="VW006_001377"
     CDS             complement(3999..4823)
                     /locus_tag="VW006_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9380220.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            complement(4958..6172)
                     /gene="thiI"
                     /locus_tag="VW006_001378"
     CDS             complement(4958..6172)
                     /gene="thiI"
                     /locus_tag="VW006_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9380221.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(6181..7323)
                     /locus_tag="VW006_001379"
     CDS             complement(6181..7323)
                     /locus_tag="VW006_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9380222.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            7461..7889
                     /locus_tag="VW006_001380"
     CDS             7461..7889
                     /locus_tag="VW006_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380223.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            complement(8040..10265)
                     /locus_tag="VW006_001381"
     CDS             complement(8040..10265)
                     /locus_tag="VW006_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9380224.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(10429..12381)
                     /locus_tag="VW006_001382"
     CDS             complement(10429..12381)
                     /locus_tag="VW006_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9380225.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(12378..13289)
                     /gene="pfkB"
                     /locus_tag="VW006_001383"
     CDS             complement(12378..13289)
                     /gene="pfkB"
                     /locus_tag="VW006_001383"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9380226.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(13286..14026)
                     /locus_tag="VW006_001384"
     CDS             complement(13286..14026)
                     /locus_tag="VW006_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9380227.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            14189..14276
                     /locus_tag="VW006_001385"
     tRNA            14189..14276
                     /locus_tag="VW006_001385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14223..14225,aa:Ser,seq:gga)
     gene            complement(14459..14806)
                     /gene="ssrA"
                     /locus_tag="VW006_001386"
     tmRNA           complement(14459..14806)
                     /gene="ssrA"
                     /locus_tag="VW006_001386"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         4471 a         3351 c         2797 g         4563 t
ORIGIN      
        1 aaccacttta aaaatgtgct gatttaatac tgcggatctt ccacaccact gaaaaacccc
       61 taatgtaccc gaccacgaaa taagaatatc accagcttca acaatgtatt ttttatcaat
      121 cgtacccgaa taataattta tcccttttga agttttagtc agattctgta ttctgataat
      181 ctctttccct tcagatgacc aatcttgagg tttaaaagca tagccattga taaaagtagc
      241 cacttgccct aatttcactt tcttcatgca accacctcct cggaatatcg ggatttgacg
      301 agcaagttta gttcatctaa ctgcaatttt cttttagtaa taagcccttt aatcgtatta
      361 agaatacaga taatgttctc ttgttcttcg atacctagca attctaattg taaatcaagt
      421 aatatattct tgtttaaatg aggaattgtt gcacctgttg aatgttctcg taaatactgc
      481 gatttacttt ccaaaaagac tcccaagtaa tctgatataa ttttttcttt gtatcgctca
      541 ttctttttta agaccgtaat tgtactacca acagctcccg ataatccata accaactgtt
      601 cctgcattag ctccatccca tgctatcaga atatcatctg ggagtgcttc agtcatattt
      661 aaactttcag tgaattttaa attattatta tttcttaaat catctatttg aatataacgt
      721 tggcttagag ttgtttgttc agcaagtaca gtggctttct tgcctttttt tagagataag
      781 acttccccca acttcacttt tttcatacag ccacctccct acttgagtaa tttttctaat
      841 tcagccaagc cagcttgaat ttctttttct aaatcattga ttttctttaa gatgacctct
      901 gttggttcat actcaacttt ttcatactca atctctttat atttattgat agacaaatca
      961 taatcatttt cctttatctc agcaactgga acaaagaaag attgatccgt tctctgacgt
     1021 tctgcttctt tttcaagatg atgaaagcgt tcgataatat ctggaatatc attgtcgcta
     1081 atcggttgtc gcttatcatc caaacttaaa ccatccgctt tcatatcgta aaaccagact
     1141 ttgtcagtac caccattacc agtttttgta aagatgagaa tggcagttga aactccagca
     1201 taaggcttga acacaccact aggcattgag attacagcat caagcttatg attctctaca
     1261 atttcctgac gaattccttt atgagcttta gacgaaccaa aaaggacacc atcaggtacg
     1321 ataactgctg ctcgtccacc tggttttaaa gttcgcaaga aaagagaaag aaagagtaat
     1381 tctgtttttt tggtttttac ggttgcaaga aggtcattag aggttgaatt gtagtcaagt
     1441 gagcccttaa aaggaggatt tgctaaaacc aaagtatatt tatcggcttc ttcattatct
     1501 tgagacagcg agtcaaggta actgatttgt ggattttcta ctccatgtag catcatgttc
     1561 atcgccccaa gtctcaacat agtcgtatcc gtatcatttc catgaaacat ctgattatga
     1621 aaatgattga tattatctgt attggtttcc cattcatctt tcttacgctt taagtaacgg
     1681 ctagcagata ctaagaagcc agcagaaccc atagcgggat ctgagatgat atctttgata
     1741 gtcggttgca tcaactcaac catcatatcg atgatgtgac gaggtgtacg gaactgtcca
     1801 tttttacctg cggtcgacaa ttttgataac agatattcat agatatctcc gatatcagta
     1861 atactttgtt tgtcattatc aaaatctaca tctaatcccc tagttggaaa aacatctaag
     1921 atagaaattg ccttttgaag cgtagcaggt ttatttattt gaaaaatagc ttctcgcata
     1981 tatcgtgaaa aggctgtatc atctgtatcc cccttgagat ttttaataaa cggaaaaatc
     2041 tcctgagtca ttaaacgata aacttcctga gcatctccta tatttttaaa agttgaccaa
     2101 cggtattcag gtttatcttt tggaaaaact ccctcataag gaatccctag aaattcagca
     2161 tcactttcac gtccaagctc gacactatcc aaatctttca taaataagag ataagtcaac
     2221 tgttcaatat ttgttaaagg atttgcgttt ccttctgtcc aaagaatttc ccacagctga
     2281 tcgattttat tttttaattc gcctgtaatc atatcttccc ttttctttag tttttaaaac
     2341 ttagtaatat cattatttta tcagatttca aacaaagctt caattaacta tatctatgcg
     2401 tcacctgaat attttatact cttcgaaaat ctcttcaaac cacgtcagct tcaccttgcc
     2461 gtaggtatat gatactgact ctgtcagttc tatccacaac ctcaaaacag tgttttgagc
     2521 aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtattaa tagactggtt
     2581 tggtatcctg aaaactatat cgtataggtg tcgtcatgga tccttttagc tgatttaagg
     2641 atacgaaaac acaatactat gactaaattg aaatgttaaa attcaatttc tagccaccta
     2701 atcctgcttc aaatagttat cgaacaagtt ttatgatagc taccttgtta ggatgctctt
     2761 attttttacc agacaaaaca aaaggattaa gggagtgaat ttccttaatc cgatagttct
     2821 tattcttgac gttgtccgaa atcagcaatc atctgatcca tttcttgtcg actcggagtt
     2881 gtcagcatat acaggtaatc tccttgaagt tccgcgaagg cagctggcat gactttttta
     2941 accttgaact gctggctata tttggcaagc aaatcctctt ctgaataaac atagtgagca
     3001 tttgcacggc gagaagtagc caaacaatac tgagtttcaa agtctgacgc cgggaactcc
     3061 tctcctgcga caatagctgc atagccacga taaaggtcca aggaatgagc aaagttataa
     3121 acatcaatgg taaaaccacc tgcagggcgg ttattgtact cgatggtaat ataatcgtcc
     3181 ccctcacgga agaactcaat atggaaaaac cgttctttca taccaaattc tttgacaatt
     3241 gcttccccat acttgcgcag tttaggatcc atatccttga gcacataata agaattgtcc
     3301 atcttataaa tcatgaggtc aagcggtgta taggcgtagt caaaggttgt tgagaagaca
     3361 atctttccat ccttgtccac gagcccgtca aaggtacaga tttcgctgga agtgacaaat
     3421 ttttcaaaga aataaagggt tgaatggtcc cattcttgct tgaagtgatt gatatcgtct
     3481 tctgtctcaa gtttaaaggt tgcggctgct cccactccat tatcaggttt ggcaatcatt
     3541 ggaagaccga tttctttcac tgcttgatca acatctgctt ccgtcttgat aacagctcca
     3601 ggtaccacag gaacacctgc ttttttgaaa agtttcttca tttcagactt atatttcgtc
     3661 tttttgagat cctctggttt ggcaccaaaa acattgaatt gttctctgag tgttgcgtct
     3721 agctcaagcc agtattcatt gtgagactcg atgcggtcaa ttggaccatg tttataaaag
     3781 agaaaagcaa ctgcacgttt gacttcatct atgttctcaa gattatcaac acgaaaatac
     3841 tcggtcaagc tattgcgcaa gggctcatcc aattgctcgt aagactcttg accaattccc
     3901 aagactgtga tgcctttatt agctagttcg atggtaaact gttgaaagtt ttgtggatag
     3961 tagggagaaa taacaaggta attcataggt aactcctttt ataaatagag attaccgagg
     4021 aaataaggca tttgtttacg ccaccattcc cagtcgtggg cgacatcatg tccccattca
     4081 gcaaaccagg ctggaatttg tttctggtca aaggcttctt tgagcttgta aaaggatggt
     4141 aaaccatctt gttcccaggc tccaagcccc gtacacagca caatctctgc ctgacggtaa
     4201 cggtcaataa accagccatc gttttggttc caaatataat ctactggcga gttttggtaa
     4261 atagcatcat cgttgtagta atcgccgaca aagaaacgtg cgtcgtaaac accactgaga
     4321 gcaatcactt tggtaaagac atctggatgc tggaggaaga aattgagtgc atgataggct
     4381 cccatagagc aacctgtcgt catcatgcca tcaaaccaac ctgttttgtg cttgataaaa
     4441 ggcttgacca cctccatgac aaactcagca tactccacac ccttaccacc aaactgctgc
     4501 tctgggatag gagattcttg gaacttccaa gccgcatact cattcatccg ccccatacca
     4561 tcattgtcaa tagcaacgac aatcatgcga ctgatatccg gatttcgttt gatagctggg
     4621 ataatcttcc atgaatgtcc aatgaaagac tctttgctat taaaaacatt ttgcccgtca
     4681 tgaaagtata caacaggata ggaacggtct gtatctttct cataatcttt aggaagaaga
     4741 atacgtacac ggcgctcctt acctgtataa ggaaccttga gtttgtgttc tttcattttt
     4801 agataaaagt aggattgatt cattgtcaga aaactctcta tattcaaaat tttatatcat
     4861 tatatcataa aaataaaaaa agttagtttt ttacctgttt ttagagaaaa aactaacttt
     4921 ttttactgat tttcgctatt cttttggatt ttctgaatta gagcagattg tcaatcaagt
     4981 catcaacttc atctttttcg gcttgaggtg tgatttcagt aatcatgatt ccagccactg
     5041 ctcgctcaac caagccttca acatccatac gcgcttcgta ctgctccgca ttcttaattt
     5101 taggatttgt ttttggacga tctggtgcaa aaatagtaca acagtcttca aacggttgga
     5161 ttgaaatgtc aaaggtatcg atttcctggg cgatgtcaat gatttccaac ttgtccatgg
     5221 taaccacagg acgaatgatg ggagtgttgg taacagcatt gatagccttc atactttcaa
     5281 gggtttggct ggctacttga cctagacttt ccccattgat gataactaaa ccatttcgta
     5341 cctcacgaat acggtcagta atacgcatca taaaacgacg cgtcaaggtc ataaggtagg
     5401 cttctggcgc tttggctttg atttcctctt gaatctctgt aaaaggaact tcgataaact
     5461 ggatatttcc cccaaatttg gttaatttac gggtcaaatc ttgggctttt ttaagagcac
     5521 caggactcgt gtaaggtggg ctggcaaagt gaactgcctc tatatctacc cctcgtttaa
     5581 gcgctagata gccagctaca ggggaatcga ttccccctga caacatgagc atgcccttac
     5641 cagaagttcc cactggtaat ccacctgccc ctcgaacggt ttcataagaa agataggctg
     5701 cttcttcacg tatctccacc tgaagattga tgtcaggact tttcatttga gcttgtacgt
     5761 ttggaatggc ttcgaataca gcccctccaa gcgtttggtt gagttcacga ctgtccaatt
     5821 caaaggtgtg gtcgctacgc ttgctagaga ttttaaaggt catgccttcc ttgtagatgt
     5881 cctgcataat ctcttggaca gcagacttca gaacttctac agatttttca accttataaa
     5941 caggggaaaa gttttgaatt ccaaatactt gtttgagcga ttctgctact gctgtgtaat
     6001 ctgctccatt gaggtaagcg tgggcacggt cgcgatctgc tgttacctta acttggggat
     6061 agatagacaa aacgtctgaa atattattgc gaagtttatt gatgaaacgc atacgatttt
     6121 tgcccttggt tgacagctca ccgtagcgaa tcataatttc tgaatactgc atgaatgctc
     6181 ctatcttact tttctagttt gattgtaaat taattttagc ttggtcaaga actgctcgac
     6241 ctgactcata tcattttcaa ggtctagact aagacgcaca gctgactgag ccttatcctt
     6301 gtccactccc atagcaatca gggttccggc tggtttccca gccttggacg aacaagccga
     6361 ggtcgttgag atgaaaatat catagtcttc aaaggcgtga acaatgacct caccacgaac
     6421 acccttgatt ccaaaagtca gaatatgagg ggcgaagtct tcctcatctg aaaagacaaa
     6481 tatatccgga tagtccagaa gtgcttgacg aatcactgcc ttcatctgcc cagtcttgct
     6541 cctaaagata tctagctttt ccatagataa acggagagcc ttagcggtcg ctgcaattcc
     6601 tgccacattt tcagttgtcg aacggtaatc acgttcctga ccaccacctg ttaaaagagg
     6661 cgtaatcttc ttgccagact tgatatagat aaaaccaaca ccacggattc cgtggaactt
     6721 gtgaccagag aaagtcgcga aatccactcg ttctgtcaga tacttttcag tcggaatctt
     6781 ggcaagcgcc tgaaccgcat caacgtggaa ggaaatagtt ggcttgtctg ccaagaattc
     6841 tgaaatagcc tcaataggtt ggatagagcc gatttcattg ttcacagcca tgatggaaac
     6901 gagggtcgta tcaggtctta tcaaatcttc tagcgcctca acatccacaa atcctttgtt
     6961 atcaactgga gcaaaatcca cttcaaaacc ttgagctttc aaccagaggg ctgattcctt
     7021 aactgctgga tgttcaatgg ctgatacgat gatgtgcttg ccaaactggg ctttttcaaa
     7081 tgctactccc ttgatgatcc agttatcccc ttctgtccca ccagatgtaa agaagatttc
     7141 atcacttttc ttaccgatta aatctgcaat ctgttgacgg gaagcatcta aaattcgtgt
     7201 tgcctggtct cccaaacgat ggagactaga tggatttcct aaaatttttg aagccacctg
     7261 catataggtt tcaagggctt ctggataggg cctggtcgtc gccgaattat caaagtaaat
     7321 catgtttctc acgctttcta aaatcactcc ttctattgta tcatgaaacg gagcctgcga
     7381 caagaaaggg caatttctct ttttttaaga tttttttaaa gaaacagagt ataataaatc
     7441 ataatcaaag gagagaatgt atgtctaact atcgtagaac ttcaaaaccg aaaacagaac
     7501 atatcaaaaa aggctttact gtttttcaaa aaaccattac tactatcggt agtatccttg
     7561 gtttgattac cgcgggtatc acgatcatga acgccttgga taataataat aaaaatacta
     7621 aaaaagaacc tacgacaagc cagacgacaa cctttgtcaa agaaattcaa aaagaatccc
     7681 ctcaggaaaa tactactcca aataaggaaa ataacacttc tcaagaaaaa acacaacaag
     7741 aagaaacgcc aaaatctagc gtcaaggaag agaaaaaaga agatcagaaa acagcaactc
     7801 aggactctac tacacctgct acaagtaaac ctgccactga aaatgaaaaa cagcccaata
     7861 ctccaacttc agaaaataat actcaatgaa aatcaaagag caaactagga agctagccgt
     7921 agacagtact tgagtacggc aaggcgaagc tgacgtggtt tgaagagatt tgcgaagagt
     7981 ataaaagtaa tcaatagcca gtaaaatagc tccttccaac cttggaaaga agctattttt
     8041 tattgctgca atacttttct tggcttggta ccttcagctg gaccaatgac acctgccatc
     8101 tcaagctctt ccatgagacg ggtcgcacgg ttaaatccaa ctgacaaacg acgctgaatc
     8161 atggatgcac tggctttctg tgtttcgata accaaagact tagcttcttc aaaaagcgga
     8221 tcaccaccag catctccatc cgaaaattct ccttcatttt cagaaacctc acctggatca
     8281 aaactctcat cgtagtctgc atctgcctga gtcttgatga agttcacaat gcgctcaaca
     8341 tcgtcatccg agataaagga gccttggaga cgaactggat gattttcatc aatcggttta
     8401 aagagcatgt ctcctcgacc aagaagtttt tctgctccat tttcatccaa aatcgtacgg
     8461 gagtctgttc ctgatgaaac cgcaaatgct acacgagatg gaacattggc cttaatcaaa
     8521 ccagagatga catcaacaga tggacgctga gttgcaagaa tcatgtggat acctgcagca
     8581 cgcgccttct gcccaagacg gatgatagca tcttccactt ccttgctggc caccatcatg
     8641 aggtcagcca actcatccac aatcacgaca atgaatggta gcggaatttg cttgtactca
     8701 gactgggaat tgaactcttc taccttggca ttaaaacctg caatattccg aactcccacc
     8761 ttggcaaaga gttcataacg gttttccatt tcatccacaa ccttttgcag agccttgctg
     8821 gctttgcgtg gattggtcac gactggaatc aagaggtggg gaatatcatt gtaaacagat
     8881 aactcaacca tcttgggatc gaccatcata aatttaactt gatctggtct cgccttcatg
     8941 agaatgctag caataatgcc gttaactgct actgacttcc ctgaacccgt tgaacctgca
     9001 actagcaagt ggggcatttt agaaaggtca aaagctcttg cggttccatt aacagccttc
     9061 cctaaaggaa tttccaagaa attttctgct ttcgtttgcg attgttccca tagttctcgg
     9121 aaagatacag tggcaatatc ggagttgggc acttcaattc cgattaggga tttcccaggg
     9181 attggtgctt caatccggac atctttggca gccaaggcta gagcgaggtc atctgataga
     9241 ttggaaatgc ggttgaccct tacaccaaca gccggcttga cttcatactt ggtcactgat
     9301 ggcccaattt cggcccgttc aactgttacc ttaataccaa agctagcaaa ggttgcttct
     9361 aagattttga tattttctct gacaattttc ttctctttag actgatcttt tggtttatct
     9421 ggtgcaaaga gttgtaagct tggaagtttg tattcaaggg cttctttggc tgaaaaatcg
     9481 acctgaacat cttcgtcatc tgagtcatct tcctgttcag ggaatttaag ttcagcttga
     9541 ggcaggatga tttctggttc cacccacttt tcttctggaa taggtggaag attttgaaca
     9601 gcttcctctg tcagaatttc acccgtttcc atatcaacag gaggcaaatc gagtaaggct
     9661 ttttcagtct cttcttgttc taatctagcc tctttctcag ccttttggcg agctttttct
     9721 tcttgtttga caaagcgttc ctcttttcga cgctcgtgcc cttcccacca tttggcaaag
     9781 cctctactga aaaattcagc aatatcgtaa acagaccaag ggctgactag gagagaaccc
     9841 actaaaatca agatagaacc aataaagtaa gttccgatat ttgaaaagag aaaagctgtt
     9901 ggaatataaa gagcgacccc gatcaagccc cctccagcaa aactagtcgt tcgaaaacca
     9961 gtcagatctg tcacaacctg agccatggtc ccttttagaa cggacttgtc caaaccatat
    10021 ttccaaacca agtaggcttc aaaaatcaag agtaagccag caaatatggt gaaaaagcca
    10081 gataagagtc cttcctgttt tcgtatccac ttgaaaaaaa agagatagat taataggccg
    10141 aatatcgcca gataagctag gctacccact agcaagcgaa ttaaattata aagggttata
    10201 cctgcagccc ctaatttgaa ggctgcgaaa atcaataaaa tcgcaattcc taacgaaatc
    10261 aacattcgtt gaatcgcttc ttttctttcc agttctgctt tagacggtct ccgtcttgtt
    10321 gtacttgtat tcttgtttgc cattcttcta ttataccata tttcacaggc atttcggaaa
    10381 gagaaaaaga ctgcaccaaa cggtccaatc ttttcatttt tctatttttt atgcttgtgg
    10441 tttgcgtagg taaccataaa ccacaccact tacgattgct cctaccaaga cagaaacgag
    10501 gtaaaggaga gcatttgaag taagggcgat aacgaagatt cctccgtgtg gcgccatgag
    10561 tttaatacca gtaagaccaa cgagtccacc tgctactgct gaaccaagga tgaagcttgg
    10621 aatcgcacga gctgggtcag cggcaccaaa tggaatcgct ccctcagtga taaatgacaa
    10681 gcccatgatg atgtttgtca aaccagagtt acgttcttcc ttagtaaatt tatctttgaa
    10741 aagaagagtt gcgacaaaga ttgcaagtgg tggcaccatt cctccagcca taactgctgc
    10801 catggctaca gaaccacctg aagaaacagt tgctgcaagc gtacctgtac caaagacata
    10861 agctgcttta ttaactggtc cacccatgtc aacagccatc attccaccaa ggacgatacc
    10921 aagaaggaca gctgaacctc ctccaagacc gcctaggaag tcattcatag cagtgttgat
    10981 tgctgccatt gggatattca cagctagcat aacaaatcct gtcaagattg ttccaagaag
    11041 tggcaataga aggattgatt tagcaccttc gagtgaacga ggaactttaa cgtatttctt
    11101 gatggcaaga accaaggcac ctgcgataaa tccaccaaca agggcaccta ggaaaccaga
    11161 tgagacacct gcaagagttg aagttgcttc accacctgcg gcataaggaa ttttaccaaa
    11221 ggcaaaacct tctttggcaa tagcaccagc cacgaaacct gctaccaaac ctggtttttc
    11281 agcaatagag taggcaacat aacccgcaaa gactggaagc atcaaaccaa aggcagctcc
    11341 accaattttc atgaacatag aagctaactc atggtaagaa ccaagattgc caaggttttc
    11401 atttggaaca cccaaagcac cgtcaatcaa gaaggcaagg gcaatcatga taccaccacc
    11461 gataacgaat ggtaacattt gagatacacc actcatcaag tgtttgtaga aggcaccacc
    11521 aaggctttgt ttttcgttag aggctgttgc agcttttgca ccattagcgg cacggtagac
    11581 ttcagtatct cctgaaagag ccaagttaat tagctcttct gtcttacgga taccgtcagc
    11641 aactggacga ttgatcaatg gttttccatc aaatcgatcc atttcaacgg ccttgtctgc
    11701 tgcaatgata atagctttag ccttacggat atcttctgca gttagttgat ttccaacacc
    11761 gctagcaccg ttggtttcga ccttgatacc aacccccatt tcagcagcta ctttttgaag
    11821 ggcttcttgg gccatgtaag tgtgggcaat acctgttgta caagctgtaa cagctacgat
    11881 aaagtcacca gagtcattag caggtacttg aacaagttcc tcagtttttt ctgaagcttg
    11941 gtcaaaaagt tcgataactt ggtctgcaga tgttgcttga cgaagtttgt ctgcaaaacc
    12001 gtctttcatc aagtattgag acaattctgc caaggctgcc aagtgagtat cattggcacc
    12061 ttctggagct gcaatcatga agaagaggtc agttgcttgt ccatccaagc tctcgtagtc
    12121 aacaccctta tttgacttag caaatagaac tgtcgcttct ttgacagcag cgtttttgct
    12181 gtgaggcatt gcgattccat cacccaaacc agtagaagtc aaagcttcac gcgccaaaat
    12241 tccttcttta aatgtttcaa aatctgttac ataactgtgc tctgtcaaat ttttaatcat
    12301 ctcgtcgatg acagctgttt tttcagttgc ctgcaaatct agcaacatga catcttttct
    12361 caataggtct tgaattttca tcttttttct acctcaactt ttccatatgt ttctttaata
    12421 aattccgccg ttgccaagtc atctgagaag gtagttgccg ttccgcaagc cactccccat
    12481 ttgaaggctt ctactgcgtc ttttgatttg acaaattcac ctgtgaatcc agcaaccata
    12541 gaatcaccag ctccaactga atttttgact gttcctttga ttggtttagc gaagtaagct
    12601 ccctcagatg tgacaagaag ggcgccatca ccagccatag agataataac attttgagca
    12661 cccttagcca gtaactcacg agcgtatttc tcaatttcat ctaaactttc gagtttaacc
    12721 ccaaaaatcg ctccaagttc atgattgttt ggttttacaa gaagaggctg gtagtccaaa
    12781 ctatcaatta aggtctgtcc ttcaaagtca cagaccactt gcgcaccagt ctggcgcgtc
    12841 aaggaaatca aatccttata gataacattg cctagatttt tagcacttga acctgcaaag
    12901 acaactgtat cttctgctgt cagactagat aaaatagctt tcaattcttc tagctgaacc
    12961 ggttcaacag ttggacccgt tccgttgatt tctgtttctt ggtctgcttt gattttaaca
    13021 ttgatacgag tatcttctgc cacctggaca aaacgtgtct cgatttcttc ctctgctaaa
    13081 gtatctgtga taaatttacc agtaaagcca ccgataaatc ccgtcgctgt atttggtata
    13141 ttcaaacgtt tcaagacacg gctgacattg attcctttcc caccagcaaa cttatcatca
    13201 ctgtccatac gatttacact accaactttg acttggtcca aacgaacgat atagtcaatg
    13261 gatggattga gtgtgactgt ataaatcata cttctattac ctccgttttc tccttaataa
    13321 cctgcaagag ctcatgccct tgactagtga taacgatagc gcgtttgagt ggtgctacct
    13381 tggcaaagca agtttgtcca atttttgacg aatccaccaa gacgtaggtc tgcttggcat
    13441 tctccaaaat agctcttttc acagctcctt cctccatatc aggagtcgta taatagccat
    13501 cgtcaacacc attcattccg ataaaggcac ggtcaaagtg caattggtta atctggttaa
    13561 gagcaacgcc cccgatacta gcatctgtcg ccgtcttgac gtttcctcca accatgacag
    13621 ttggaatctg cttttcaacc aactgagcgg catggtgaat ggagttggtc acaactgtaa
    13681 cattcttatt gaccaattca tgaatcaaaa aagcagttgt tgttccagca tcgataaaga
    13741 tgacatcttt ttctttaatg agagaggctg ctttctgagc cagcaacttc ttttcttgaa
    13801 ggtttttgac agatttttct tgaatggttt cttcttcctg taaggagtgg gggagttctg
    13861 ctccaccatg cacacgacga agcttgtttt ccgcttccaa ctcatccaag tctcttcgaa
    13921 ccgttgattc tgacgtttct agcaaactaa ctaatttttc tagagaaact acatgatgtt
    13981 gatttaactc ctctaaaatt agttgtttac gttctgtttt taacactgaa tcacctcctg
    14041 ttatcgttta cactattcat tctatcatac tttctttcga agtcaagcat tttttatcat
    14101 tttctttcaa aatctatcat tttttcttat ttctagaatt tttattgcaa gataagagcc
    14161 tttatggtag actatttgag taagtattgg aagattactc aagaggctta agaggccgtg
    14221 ttggaaacgc ggtaggcgtg taatagcgtg cgtgggttcg aatcccatgt cttccgtcga
    14281 aaaaagatta tttctaggat gcgacaaatg ttgtatcctt ttttctatcc tttttttaaa
    14341 atagggtctg tttgtgactc cctttttagc aaaagaatgc aaaggaatac caaagataaa
    14401 aataaaaaac gttgatttaa caacgtttta ccaaggaatg caaaagaatg caaaggaata
    14461 atggagccgg tgggagtcga acccacgtcc aaacacctgc caacatattt gtctacaacc
    14521 ataggttatg tattatttta acaacccctc gacacataac tcaagtctag aaactgcgag
    14581 tctatcaatc tcttatcaag ccgctagaca aggcttaatc gtatctcgct aataataaga
    14641 cctgtcattg aacacgagca atccaaatcg ggtcacgcct gctggttttt aggcagctag
    14701 agcgtaagaa gtgttatttt ttgcagttat atttaactga gcgtttacgt cgccacacga
    14761 gtcgcaaaat atgcctcata atgcctgtcg aatccgtaac gaccccaaaa gacaataaaa
    14821 ccattatacc ttatctaact caaaaatgca aaaagaaatc acatacctaa aaaagatact
    14881 attcgagaaa tttagcaaac tggaagacta actacaactt tctcagtgca taactttacg
    14941 attgtaaata gagcttacga aatccaatta acacatacct aagatgaagc tatctgtttt
    15001 gaagaaattc acgaagagaa ttattctaaa atactttcag atgagaattt tcgtactctc
    15061 tttattttaa ttcatttgag tttaaaaact ataaaatcaa tccttatatt attcggacta
    15121 cttttcactt attgcaccaa aatatagtaa aatgaaataa gaatagtacg aatagatcag
    15181 ga
//
LOCUS       DAVKFH010000037        15163 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKFH010000037 DAVKFH010000000
VERSION     DAVKFH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.037"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            153..452
                     /locus_tag="VW006_001387"
                     /pseudo
     CDS             153..452
                     /locus_tag="VW006_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            535..2556
                     /locus_tag="VW006_001388"
                     /pseudo
     CDS             535..2556
                     /locus_tag="VW006_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            2558..3199
                     /locus_tag="VW006_001389"
     CDS             2558..3199
                     /locus_tag="VW006_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380228.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3303..4655
                     /locus_tag="VW006_001390"
     CDS             3303..4655
                     /locus_tag="VW006_001390"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9380229.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4659..5717
                     /locus_tag="VW006_001391"
     CDS             4659..5717
                     /locus_tag="VW006_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9380230.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5727..6926
                     /gene="divIB"
                     /locus_tag="VW006_001392"
     CDS             5727..6926
                     /gene="divIB"
                     /locus_tag="VW006_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9380231.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            7159..7860
                     /gene="pyrF"
                     /locus_tag="VW006_001393"
     CDS             7159..7860
                     /gene="pyrF"
                     /locus_tag="VW006_001393"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9380232.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAAAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            7894..8526
                     /locus_tag="VW006_001394"
     CDS             7894..8526
                     /locus_tag="VW006_001394"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9380233.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            8706..9109
                     /locus_tag="VW006_001395"
                     /pseudo
     CDS             8706..9109
                     /locus_tag="VW006_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9205..9969
                     /locus_tag="VW006_001396"
     CDS             9205..9969
                     /locus_tag="VW006_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380234.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            9969..10721
                     /locus_tag="VW006_001397"
     CDS             9969..10721
                     /locus_tag="VW006_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380235.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            10731..11429
                     /locus_tag="VW006_001398"
     CDS             10731..11429
                     /locus_tag="VW006_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380236.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            11426..12073
                     /locus_tag="VW006_001399"
     CDS             11426..12073
                     /locus_tag="VW006_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380237.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(12153..13014)
                     /locus_tag="VW006_001400"
                     /pseudo
     CDS             complement(12153..13014)
                     /locus_tag="VW006_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(13026..13784)
                     /locus_tag="VW006_001401"
     CDS             complement(13026..13784)
                     /locus_tag="VW006_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9380238.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(13785..14462)
                     /locus_tag="VW006_001402"
     CDS             complement(13785..14462)
                     /locus_tag="VW006_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9380239.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(14422..15090)
                     /locus_tag="VW006_001403"
     CDS             complement(14422..15090)
                     /locus_tag="VW006_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9380240.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
BASE COUNT         4550 a         2624 c         3291 g         4698 t
ORIGIN      
        1 aaaatatcta aaaaattcga ttttttcctt gacaacaaat aataaaaatg ttattcttat
       61 cacataagaa agcggtttca ataaaagaga gaggaggatt tattttgttg cattgttgct
      121 caaggtgtga tttttttgaa agaaaaaata aaatgaaaac gaaaaaacat agattacttg
      181 ctctagctct tatttcaagt tttacattat tgggagctgc atcagctgct gtataatatc
      241 cagatggagg agtatggaca tatggagaag gttcaggagg tggttaggct ttttcaaatt
      301 actatcatgg taaaaaatat cattattcat ccatcgtgag cagatgggat ggtcattctg
      361 ataaaggaga agctcctgct ggaaaaacct cctatgcatg gatttggact aaatggggag
      421 aacaagtagc attttactgt gattatgact aatcgttcat tgatttaaat cgttcccctt
      481 gtaagaaatt acagggggaa ttttgtttac aaggagtcta ccaaggagtc tactatgaag
      541 aaactattca tattattatc aacttttttt ctcagcttct tccttgcttg gattattgtc
      601 ttacgtgcgc cacaatattt atatgcaagc tatgattccg tttctttact tcgtgtcaaa
      661 aaagatactc aggaaccgac gcgtgaggta tttgaacagg aattggagaa ttttgcaaac
      721 tcagaacaga gtttaatagc tagaagaatt gtagagccga gtaaggatgg gacgactcac
      781 tttacttatg caacttatgg tcagggaact ttaccaaaag aattccaaga agctagccaa
      841 gaaagtcgtg aacgtagtga tccgctaaat agttatctcc ttttgtcagg ctccttgacg
      901 aaagaaaagc ttgccgataa attaggagat ttgggttata aagcaagtgc tgaccgaaag
      961 ataccgccct attttcttgc ttttcgaata ttactaaatc cccttatttt aattagttta
     1021 gcaatatttg gcttatcttt ctttgcttta gtgattatca ctcggattaa ggaaatgaga
     1081 gcagcaggta taaaactctt ttctggtcag actctcttat ccatcatggg gcattcttta
     1141 tctactgata tcaaatagct ccttctatca gccctccttt ccttcctagg tgggggagtc
     1201 gttcttttta gtcaaggttt gttttatcct atcttgttag ccacctatgg ttttgggatt
     1261 agtttctatc tgttgttttt attggcgatt tcaattttac taatgcttct ttatctaatg
     1321 agtttgagtt acaaagcatt agttcccgtt attaagggga gattacccct taaacgcctg
     1381 atgattttaa ccctattgtg tcagttggtt gctgtcttta cagtaggata cgctgttaag
     1441 acaggtttga cgtcttacca acgattgaaa gaacttgaaa tttcaaaaca agcatggcag
     1501 gatagagcag actattatca aatttctttt ggcttaggtg atagaggaaa agatacagaa
     1561 aatcagagca agtggtatgc ctttgccaag gaagcaatcg aagaggaaca agctctttat
     1621 gtaaaggata atctgctcca ttttgccaat ccacaaggaa aaaatgaaca gggagagaca
     1681 ctggatacct atagtccaga tgctaatacg ctctatgtta gtcccagtta tttggacaag
     1741 gaaaaggtcg tggtagatgc tgagaccaaa cagaagttag cccatctcca aaaaggtgag
     1801 tttatcctct tgctcccaga acatttgcgc tctcgagaag cagaacttaa gaaagttttt
     1861 gaagaaagat tgagttatta tggaaaatct ggtgaggagg caagtgctcc tttggattat
     1921 gagatgaaag cgcacgttag ttatctttca atgggagaaa agcggtttgt ttataataac
     1981 ggtgagaatc ccgtatctac tcagtatttg actgatccga ttttagtggt attcacgccg
     2041 acttctacag gtgatagttt tatatcctta tctagttggt ctatcaatgc tggaaaacaa
     2101 ctctttatca aaggatatga gagtgggcta gaactcttga agaaagctgg aatttatgag
     2161 caagtatcct atcttaaaga aggaagaagt gtttatctaa ctcgttataa tgaagttcaa
     2221 actgaaacag caactttaat cttaggagct attgtgggga tagctagttc cttattacta
     2281 ttttattctg tcaatcttct atatttcgag caattccgcc gagatatctt gattaaacga
     2341 atttcaggtt tacgattttt tgaaacacat gctcagtata tggttagcca atttgccagt
     2401 tttgtatttg gtgctagtct ctttatttta agcagtcgag acttggtgat tggcttgctc
     2461 actttattgg tctttctagc tagtgcagtt ttgacgcttt accgtcaagc gcagaaagaa
     2521 tctcgtgttt ctatgacaat tatgaaagga aaataggatg attgaactaa agaatatatc
     2581 taaaaaattt ggaagccgtc agctattttc agatacgaat cttcattttg aaggtgggaa
     2641 aatttatgcc ttaatcggta caagtggctg tggtaagaca acactcttga atatgattgg
     2701 acgattggag ccatatgaca aagggcaaat catctatgat ggcacttctc ttaaggacat
     2761 caagccttct gttttcttta gagattactt aggatactta tttcaagatt ttggcttaat
     2821 tgaaagccaa accgtcaaag agaatctcaa tctgggttta gttggtaaaa agttgaagga
     2881 aaaagagaaa atctctttga tgaaacaagc tctaaactgt gtaaacctct cttatttgga
     2941 tttgaagcaa cctatatttg agttatcagg aggagaagca caacgtgttg cactagcgaa
     3001 gataatttta aaggatccgc ctttgattct tgcagatgaa ccaaccgctt ccttagaccc
     3061 caaaaattct gaggaattac tttccatcct agaatcttta aaaaatccga atcggaccat
     3121 tattattgcg acccacaatc ctctgatttg ggagcaagtg gatcaggtca ttcgagttac
     3181 cgatttatca catagatgat atggtaagat tcagttagaa gaaagagtca caaacacact
     3241 ttgtggcttt tttatttcca taaaaatggt aaaatagtag gagtagaaat ggagttcgag
     3301 acatgaaagt aatagatcaa tttaaaaata agaaagttct tgttttaggt ttggccaagt
     3361 ctggtgaatc tgcggctcgt ttgttggaca agttaggtgc cattgtgaca gtaaatgatg
     3421 ggaaaccatt cgaggacaat ccagctgccc aatgtttgct ggaagaaggg atcaaggtca
     3481 ttacaggtgg ccatcctttg gaactcttgg atgaagagtt tgccctcatg gtaaaaaatc
     3541 caggtatccc ctacagcaat cccatgattg aaaaggcttt ggccaaggga attccagtct
     3601 tgactgaggt ggaattggct tatttgattt cagaagcacc gattattggt atcacaggat
     3661 cgaacggtaa gacaaccaca acgactatga ttggggaagt tttgactgct gctgggcaac
     3721 atggtctttt atcagggaat atcggctatc ctgccagtca ggttgctcaa atagcatcag
     3781 ataaggacac gcttgttatg gaactttctt ctttccaact catgggtgtt caagaattcc
     3841 atccagagat tgcggttatt accaacctca tgccaactca tatcgattat catggattat
     3901 ttgaggaata tgtagcagcc aagtggaata tccagaacaa gatgacagca gctgatttcc
     3961 ttgtcttgaa ctttaatcaa gacttggtaa aagacttggc ttccaagaca gaagccactg
     4021 ttgtaccatt ttcaacactt gaaaaggttg atggagctta tctggaagat ggtcaactct
     4081 acttccgtgg tgaagtagtc atggcagcga atgaaatcgg tgttccaggt agccacaatg
     4141 tggaaaatgc ccttgcgact attgctgtag ccaagcttcg tgatgtggac aatcaaacca
     4201 tcaaggaaac tctttcagcc ttcggtggtg tcaaacaccg tctccagttt gtggatgaca
     4261 tcaagggtgt taaattctat aacgacagta aatcaactaa tatcttggct actcaaaaag
     4321 ccttatcagg atttgacaac agcaaggtcg tcttgattgc aggtggtttg gaccgtggca
     4381 atgagtttga cgaattggtg ccagacatta ctggactcaa gaagatggtc atcctgggtc
     4441 aatctgcaga acgtgtcaaa cgggcagcag acaaggctgg tgtcgcttat gtggaggcga
     4501 cagatattgc agatgcgacc cgcaaggcct atgagcttgc gactcaagga gatgtggttc
     4561 ttcttagtcc tgccaatgcc agctgggata tgtatgctaa ctttgaagta cgtggcgacc
     4621 tctttatcga cacagtagcg gagttaaaag aataaaatat gaaaaaaatt gtctttacag
     4681 gtggggggac ggttggacac gttaccctca atcttttgtt aatgcccaag ttcatcgaag
     4741 atggttggga agtccactat atcggggaca agcgtggtat cgaacaccaa gaaatcctta
     4801 agtcaggttt ggatgtcact ttccactcca ttgcgactgg gaaattgcgt cgctatttct
     4861 cttggcaaaa tatgctggac gtcttcaaag ttggctgggg aatcgtccaa tcgctcttta
     4921 tcatgttgcg acttcgtcca cagacccttt tttcaaaggg gggctttgtc tcagtaccgc
     4981 ctgttatcgc agcgcgtgtg tcaggagtgc ctgtctttat tcacgaatct gacctgtcta
     5041 tgggcttggc caataaaatc gcctataaat ttgcgactaa gatgtattca acctttgagc
     5101 aagcttcgag tttgtctaag gttgagcatg tgggagcagt gaccaaggtt tcagatcaaa
     5161 aaaatccaga accagatgaa ttggtggata ttcaaaccca ctttaatcat aaattgccga
     5221 ctgtattgtt tgttggcggt tctgcaggtg ctcgtgtctt taaccaattg gtgacagacc
     5281 ataagaaaga actaacagag cgctacaata ttatcaatct aactggagat tctagcctga
     5341 atgagttgag ccaaaatctt tttcgtgttg actatgtgac cgatctctat caacccttgt
     5401 tggaattggc tgatattgtt gtgacacgag gtggtgccaa tacgattttt gagctcttgg
     5461 cgatagcaaa attgcatgtc attgtgccgc ttggtcgtga agctagtcgt ggtgaccaga
     5521 ttgaaaatgc agcttacttt gtaaaaaaag gctatgcaga agaccttcaa gaaagcgatt
     5581 tgaccttgga tagtttggaa gagaagcttt ctcacttact aagtcacaag gaagattacc
     5641 aagctaagat gaaagcttct aaggaattga aatctctagc agatttttat caattgttga
     5701 aaaaagattt atcataagga aagtaaatgt caaaagataa gaaaaatgag gacaaagaaa
     5761 tcctcgaaga attgaaagag ttatcagaat ggcagaaacg aaaccaagaa tatctaaaaa
     5821 agaaggctga agaagaagcg gctctagctg aggagaagga aaaggaaaga caagctcgga
     5881 tgggagaaga atctgagaag tcagaggaca aacaggacca ggagagtgaa acagaccagg
     5941 aagattcaga atcagctaag gaagagtctg aagaaaaagt agcatcctca gaggatgaca
     6001 aagagaaaga agagaaagaa gaaccagagt ctaaagagaa ggaggaacag gataaaaagc
     6061 ttgctaaaaa ggctacaaag gaaaaaccag ccaaagcaaa gattcctggt atccatatct
     6121 tgcgagcctt cacgatttta tttccaagtc tgcttttatt gattgtctct gcctacttgc
     6181 tcagtcctta tgcgaccatg aaagatattc gtgttgaggg aacggtgcaa actacagctg
     6241 atgatattcg acaggcttca ggcattcagg attcggatta tacgattaac cttctgctag
     6301 acaaggcaaa atatgaaaag cagattaagt ctaactattg ggttgaatca gctcaacttg
     6361 tctatcaatt tccaactaag ttcactatta aggtcaagga atatgatatt gtggcctact
     6421 atatttctgg tgaaaatcat tatcctattc tttccagtgg tcagcttgag actagttctg
     6481 tgagcctgaa cagtttacca gaaacttatt tatcagttct ctttaatgat agtgaacaaa
     6541 tcaaggtttt tgtctcagaa cttgctcaaa ttagcccaga actcaaggcg gctatccaaa
     6601 aggtggaatt agccccaagc aaggtgacat ccgatttaat tcgattgact atgaatgatt
     6661 tggacgaagt cttggttccg ctttctgaaa tgagcaagaa actgccttat tatagtaaga
     6721 ttaagccaca attgtcagaa ccgagtgtga ttgacatgga agctggaatt tacagttaca
     6781 ctgtggcgga taaattaatt atggaagctg aggaaaaagc caaacaagag gctaaggaag
     6841 ctgagaaaaa acaagaagaa gaacagaaaa aacaagagga agagagcaat cgaaaccaaa
     6901 cgacccagcg ctcatcgcgt cgctaggttt accttttctc ttatagttct ttagtgacca
     6961 tgtttttact tgacaagtgc tagtagaaat aatagaatag taaaaaacct ttaaagcagt
     7021 ccagagaggc agctaaggtt agacggtgaa agggtggaga ctacccattt ttcgtggaac
     7081 cttgctgttg gcaggttcct tttttcgtgg cttctgttgg ccagactctc tcactagtaa
     7141 aggtaaaagg agaaacctat gcgagaacat cgtccagtca ttgctcttga ttttcctagt
     7201 tttgaggcgg tcaaggaatt tttagctctt ttcccagcag aagaaagcct ttatctcaag
     7261 gtagggatgg agctttatta cgcagcgggg cctgagattg tgtcctactt aaaaggtttg
     7321 ggtcatagtg tctttttgga tctcaaactt catgacattc ctaatacagt caagtcagcc
     7381 atgaaggtct tgtctcagct tggtgtcgat atgactaatg tccatgcggc tggtggtgta
     7441 gagatgatga aggcggcgcg tgaaggcctt gggagtcaag ctaaattgat cgctgtaact
     7501 cagctcacat caacgtcaga agctcagatg caggagtttc aaaatatcca aaccagtctg
     7561 caagagtctg tgattcacta tgccaagaag acagctgcag ctggcttgga tggtgttgtt
     7621 tgctcggctc aggaagtaca agtcatcaag caggctacca atccagattt tatctgtctg
     7681 acaccaggga ttcgtccagc tggtgctgca gttggagatc aaaaacgagt catgacacct
     7741 gctgatgcct atcaaatcgg cagtgactat atcgtagtgg gacgtcccat tacccaagct
     7801 gaggatcctg ttgcagctta tcataccatc aaggatgaat ggacacagga ctggaattaa
     7861 agaactagat tagaaaaata aaaggagaat accatgacac ttgctaaaga tatcgctagc
     7921 cacctcttga aaatccaagc cgtttacctc aaaccagagg aacccttcac ttgggcatct
     7981 ggtatcaagt caccgattta cactgataat cgtgtgacac tagcctatcc agaaactcgt
     8041 accctaattg aaaatggttt tgtggaagct atcaaagaag cctttcctga agtagaagtg
     8101 attgcaggaa ctgcaacagc agggattcca cacggagcca ttattgctga taagatggac
     8161 ttgccttttg cctacatccg tagcaaacca aaagaccacg gagctggtaa tcaaatcgaa
     8221 ggtcgcgtag ctcaaggtca aaaaatggta gtggttgaag accttatttc aacgggtggt
     8281 tcagttcttg aagctgtagc agcagccaag cgagaaggag cagatgtgct tggagttgta
     8341 gcgattttca gctaccaatt gccaaaagca gataagaact ttgcagatgc tggtgttaaa
     8401 cttgtgacgc tttcaaacta tagcgagctt atccatctag cccaagaaga aggttacatc
     8461 acgccagagg gccttgatct tctaaaacgc tttaaagaag accaagaaaa ttggcaagaa
     8521 ggttaggtca gtaagataaa gagagatgag gctaccgagc ctcttttatc attttattta
     8581 aaatatgaca atattttaaa taaaatgatt gacaacgctt tcttttgttg atataatgaa
     8641 aatgtaatat gtatcaaatt gatcaatcaa ctatcttgtt tatcacttct taggaggacg
     8701 atgtcatgaa aagaagaaaa atatattcct tttttgcgta gttacaattg catctagttt
     8761 gtttctcctt acagttttag ctagacagat tggagattat gatttaacgt tgccgcgttg
     8821 gggttcggat acaactagtg agcttgagaa agaaaacgcc tctgctggaa ttaataataa
     8881 tgacagcact ggtggcggta aaaggttaaa tacctctatt cgtagcgcct atagtgggtc
     8941 agatattacc ccggtatatt cattggggtc tggctctagg attgtcatgt actataatgg
     9001 aggtggtgac aattatattg gttctggtac tagattagct atggcgccac aatttggaaa
     9061 tcatgtaaga attcatactt caggttcttg gaatccagat tcttattaac ttacttgtca
     9121 gagtaagcct tagagatggt tgattgtggg tgtagcatga aaaaagaatg ctacacccta
     9181 tttttattat aaggaggagt aaggatggaa tttttcattt gtaatcttgt acgagtcgtt
     9241 caatcacctc gattttatat gtctttattt ttgacccttc tttgcatgag tttaggaaat
     9301 ttccttgctt tcaatggtat ttataaaatt gaaggtttat cgattttttt tgccgcttct
     9361 tctattcgag gattttcacc gattagccta gtagctgcac ttatctgtac actgccctat
     9421 tctagtcaga taatagagga tgctgagagt cattttctaa cagcacaatt gtgtcgaatt
     9481 tctaaaaaga agtatctggc tattgtgggt agtactgtaa ttatttcttc ttttctagtc
     9541 ttttttctcc cctatttatt attattagga attaatcttt tagtgactcc ttatcaggaa
     9601 atttatattg gagattatag tggtgcctta aaagaattat ttgattccaa tcagtttctc
     9661 tatagtcttg taacgactct ctggtatgga gtttggggcg ctgtgttctc tatttttgga
     9721 ctagctagtg ctttgctagt gaagaaaaaa ataggagcta ttttcatccc agttgcctat
     9781 atgatggttg gtggtatttt ttgggctatt ttagggctat cttacttaga acctgtgaca
     9841 acattagctt tgggatatca gaaagatatc agtctttcct tagttagtgg gcatcttgct
     9901 tttattttat ttgttagttg tttggttgtt tatggtacat tttttctaca ttcagaggac
     9961 tacgtataat gaaacaattt gttcaatttt acaaaaaaga ttttttatca gtattggttt
    10021 attttatatt actgctatcc tgtgttttat ctagtacagt atatttattg cgctgtcgcc
    10081 aatattcaat ccatccaaat gtattagaat ggatcttagt tttacttcaa gatatgacga
    10141 ctggagtata ttgctttccg ttcacatata tattgttctt tttttatttg atgaataact
    10201 attttaatag gttggagtgt cgcattcgtc tgaaatcaat taagcacttt accagtttta
    10261 gtttcaaatt agcagctctt agtacgggga tttggacggc gactttattt ttattgattt
    10321 ttctaattgc atttagtaat ggttttagct tctctttgga gataaaggag gttgattttt
    10381 taagagaatt ttatggtata agtattgcaa acaatgctag tttctttata ggattttttt
    10441 tctcttatat agcatactat ttctttttat ccttacttac tattagcagt ttttcttggt
    10501 ttaaaaaatc aaacatgagc ttagtatttc tgtttacttt tttatttgta gaatccttat
    10561 tctggattta tcagttggac aatgggataa ttggattatt gccaattttt cagtatatgg
    10621 taaattccaa tccgtatgca ttgatttatt ggcttacatt actatctatc ataattccat
    10681 tgactgtatt ttctgttcat agaaactgga ggagagtgta aaagttggaa atgggaaagt
    10741 taagtagtca catgtggagg ttgaatcaga taatctatac caagtacttt tggggttatg
    10801 ttcttttttg gatattgatt tgtttaggat tatggtattg gttagaagga aatgatagac
    10861 ttgttataga aattttaaaa gggcctaatc tgagtcaaaa ctctttttta gtcttatcta
    10921 tatggttgct tcattggttt attattcata cattttttct agcagttgta tatcgtagaa
    10981 gagcatccga tttctttatg gaagtgattc gattttcttc tattaagctc tggattaggt
    11041 atcagatttg gacctgtttt ctttatggac tcattttaat catggtaaaa gttctagtga
    11101 ttcaatttat gttacagtta ccaaactggg atataggagt tttgtttata gttgattctt
    11161 tgaatgcttg tgtgttagtc ttgttttgct ttatgttata cgcactagga gcgaatgtac
    11221 aaatgaactt tgcttgcgtt agtttctttt tactcatgat tgtgtttggt ggtctatttg
    11281 taggaaatcg aactaattat ctattttata ttttgaatcg agggaatgga gatataggac
    11341 gagatttatt tcttcagttg ctatttttag tctttctttt taaaagtatt ttctatttca
    11401 ctcgtcaaaa gaggaggttt atagaatgaa tgaaataatc atattaaaaa atattgagtt
    11461 gaaattaaaa aaaacatgtg tttttcaaaa ccttaatttt agttgtaaac agggggaaat
    11521 tataggaatt actggtgcga atggctcagg gaaaagtgta ttgtttaaat taatagctgg
    11581 tttatatagt ccgtcttatg gagaagtgtt aatcaatggg gaaaatattg ttcctgagag
    11641 aaaaattcca gctaatttgg gagctttgat tgaagaacct ggttttataa attattatag
    11701 tggctttaag aatttacaat atttggcaag catacgagga gtagttggta atcaggaaat
    11761 caatgataca ctgaaaatag ttggtctata tgagcaaaaa gaccagaaag ttaaaactta
    11821 ttcgctaggt atgaggaaaa agctagggat tgctcaagca attatggaga atccctctat
    11881 tcttttacta gatgaaccta tgaatgcctt ggataaatca agtgtagaaa atatgagaac
    11941 attgtttaga aagctctcta gtgaaaaagg gacaacaatt ttgattgcta gtcatagtga
    12001 agaggatatt cgtatcttat gtgataaagt atatgcaata gaagataaag tatgtacact
    12061 gtgttcagat tgaacatctt agatagactc aggatgttat aggtaaaatc ttaaaaaggg
    12121 attggagctt aaaatccagt cccttttaag agttaatggc caccttcaac aaccaggtca
    12181 tctgctttag cagcgtcacc gtaggttggg tgaagtgttt tttcataggc cttgtggaag
    12241 aagttttcct tgcctaattt ttcaatatct ttattgatga agtctagcaa ttcttggttg
    12301 cctttttgaa ctgctgccgc aatggtatcg ggatcaccga gggaagtaat tcctacttca
    12361 aatcctttat tttcaagcgc ccaagctaga acttccgtat tgtcagttga aaaggcatct
    12421 ccacgtccgt caagaagagc ttggtaagag tcactgtatt ggtcgtattt ttggagtttg
    12481 atttctggat gattcttttc aaaataagtc tcagcagtcg ttccttttgt gacaattaag
    12541 gttttacctt caagttgttt gacgtctgta atgagaccag tcttaggtga tacgacaccc
    12601 agagaaactt tcatatatgg aagggcaaaa tcaacttgtt tcttacgttc gtcagttact
    12661 gtaaagttag caagagtaat atctaccttg tttgaaatca agtattccgc acggttggca
    12721 gcatcgactg aaatgtattt aaccttgaca ccaaggtctt tagctagttg gttccctagt
    12781 tcaatatcgt agccttggta agaaccatca ttgtcaacgt agccaaacgg ttttttatct
    12841 ccaaacacgg cgattcgcag ttcaccgcct ttttttgatt tcatcgatag tgcgagcttt
    12901 ggcagttgtt tttccagagg atgaaccagc gtttccacct gagctacaag cagtgataaa
    12961 gataagggca aaggcaagtg ctaaaacagt taagagtggt ttgaatagtt tcataagaat
    13021 ctcctttata gatatgagcc aaattggcta aagtcaaaga cgtttaaaaa ttcctgggct
    13081 cgtttggttt gcggattggt aaagaaggct tgagctgttc cttcttcagc gattttccct
    13141 tggtcgagga agataatccg gtcagcaatg gcttgggcaa actgcatttc gtgggttact
    13201 aaaatcatgg tacggccttc ttgggccaaa tcattgataa gttccagcac ctcacgcacc
    13261 atttctggat ccagcgaagc agtcacctcg tcaaaaagga tgatttctgg atgcattagg
    13321 agggcacgga caattgcaac acgttgcttc tgtccaccag ataattgacg ggcaaagcta
    13381 tgttgtttat ccagcaaacc gacacgatct agtaattgca aagcttcttc cgttacttct
    13441 ttcttgtccc ttccttgagt tttgataggg cctaggatga ggttttgtaa gacatccaga
    13501 tggggaaaga gttcataact ttgaaagacc atgccaatct tttggcgaac taggtgaaaa
    13561 ttttttttat tttcaacgat agactgacca tccagaaaaa tatctccacc ttgaatactt
    13621 tctaagccgt tgaggcaacg aaggagggta cttttcccac aaccagatgg ccctaggatg
    13681 acaacaactt cccctttttt gatttctaga gaaagtcctt ggaggatggg attgtctccg
    13741 aaggattttt ttagttcctt gatttctaag atagtttcag acatttagtt tctccaatgt
    13801 ttttctaagt gagtggatag tttggaaata gggtagcaaa ctgcgaaata taagactaga
    13861 atggttccat aaatccaaaa tgaagcagtt gggatggtca ggcgattgct atcgatgatt
    13921 tgttgtccaa ctttggtcac ttccacaacc ccaatcaaaa caactaatga agtggtttta
    13981 atcatccgag tgacaagatt gatagcctgc ggtagcagtc ttctcaagac ttgtgggatg
    14041 atgatgtggt agtaaagttg aacattagtc aagccgagtg cctgtccact ttcaaactga
    14101 tgtttaggga gagaagtgat agctccacgt accaagtctc ccatttcagc tgttccccag
    14161 agggtaaaaa cgataatagc tgaagtctca cctgagatat tgatattaaa gtttcgagcc
    14221 aagccaaagt aaacgatgaa gagtagcacc agctggggca tgatacggat aaattccaga
    14281 tacaatcgtg ttaaaaatcg tatgattcta gaatgggagg tcatgatgat tcccatgact
    14341 gttccgaaca tcatggataa gaggacagac aggatggata tcccaatcgt aacgcccaat
    14401 ccctgtaaga ttctcaggag attatttcct tgaaagagta cttggattcc cgaatcctgc
    14461 atggcggagc ctcctttcta tccagctaaa gaccagtgag atgggtagca gcatgatcag
    14521 ataagcaact accaacatag ctagcgcaat gtctgtctca tagtagagac caatcaaatc
    14581 cttggcgacg tacatgaggt cggccaaagc cactgctgag aaaacagagg tttccttgat
    14641 aaggaaaatg acattggcac taaaggaggg gagtgccacc gctgttgctt gcggaagaac
    14701 cacatagtaa aagacctgta gaggtgtcag accaatagcg aggccaatct cctgctgggt
    14761 ttgactgatg gcttccagcc cgcttcggaa agattctgcc atataggagt ctcctaaaaa
    14821 gacaagccca agcgttgcac agacttctga agataggaca atcccgattc ggggaagacc
    14881 gaagtagaga aagaagagtt gaatcaaaag gggcgtatta cgtgacaatt caatgtaggc
    14941 tgtcgctact tgcgacaaaa caaggattcg ataatgtcgg atgatactaa cgattaaacc
    15001 gagcagaaag gatcccaaaa ttccccaaac tgcaatatgc aaggtcagaa agaatgcctt
    15061 ttgatatagt ggtagatatt gttcaacaat ggatcaatcc aaaaatagaa cctcccatct
    15121 agaaataata cagttattgt agcacttaaa atcttctttg gat
//
LOCUS       DAVKFH010000038        14555 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKFH010000038 DAVKFH010000000
VERSION     DAVKFH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.038"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            88..1026
                     /gene="msrB"
                     /locus_tag="VW006_001404"
     CDS             88..1026
                     /gene="msrB"
                     /locus_tag="VW006_001404"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9380241.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            1084..2808
                     /locus_tag="VW006_001405"
     CDS             1084..2808
                     /locus_tag="VW006_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380242.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            2810..4558
                     /locus_tag="VW006_001406"
     CDS             2810..4558
                     /locus_tag="VW006_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380243.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDTFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            4905..6164
                     /locus_tag="VW006_001407"
     CDS             4905..6164
                     /locus_tag="VW006_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9380244.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    6240..6375
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            6414..6914
                     /locus_tag="VW006_001408"
     CDS             6414..6914
                     /locus_tag="VW006_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9380245.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            6990..7358
                     /gene="rplL"
                     /locus_tag="VW006_001409"
     CDS             6990..7358
                     /gene="rplL"
                     /locus_tag="VW006_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9380246.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            7545..7715
                     /locus_tag="VW006_001410"
     CDS             7545..7715
                     /locus_tag="VW006_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380247.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            7715..7963
                     /locus_tag="VW006_001411"
                     /pseudo
     CDS             7715..7963
                     /locus_tag="VW006_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            7977..8317
                     /locus_tag="VW006_001412"
                     /pseudo
     CDS             7977..8317
                     /locus_tag="VW006_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8310..8690
                     /locus_tag="VW006_001413"
     CDS             8310..8690
                     /locus_tag="VW006_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9380248.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            8703..8846
                     /locus_tag="VW006_001414"
     CDS             8703..8846
                     /locus_tag="VW006_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380249.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            8939..9526
                     /locus_tag="VW006_001415"
     CDS             8939..9526
                     /locus_tag="VW006_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9380250.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            9728..9865
                     /locus_tag="VW006_001416"
     CDS             9728..9865
                     /locus_tag="VW006_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU9380251.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            10044..12653
                     /locus_tag="VW006_001417"
     CDS             10044..12653
                     /locus_tag="VW006_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9380252.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKSMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            12686..>14555
                     /locus_tag="VW006_001418"
     CDS             12686..>14555
                     /locus_tag="VW006_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU9380253.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGISSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRAPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAADHGGFT"
BASE COUNT         4574 a         2423 c         3073 g         4485 t
ORIGIN      
        1 aacatatata cggtaaggtg acgctaacgt ggtttgaaga gattttcgaa gagtattagt
       61 taaaaacgtg ataaaggaga aataaagatg gcagaaattt atctagcagg tggttgtttt
      121 tggggcctag aggaatattt ttcacgcatt tctggagtgc tagaaaccag tgttggctac
      181 gctaatggtc aagtcgaaac gaccaattac cagttgatca aggaaacaga ccatgcagaa
      241 acggtccaag tgatttacga tgagaaggaa gtgtcactca gagagatttt actttattat
      301 ttccgagtta tcgatcctct atctatcaat caacaaggga atgaccgtgg ccgccaatat
      361 cgaactggga tttattatca ggatgaagca gatttgccag ctatctacac agtggtgcag
      421 gagcaggaac gcatgctggg tcgaaagatt gcagtagaag tggagcaatt acgccactac
      481 attctggctg aagactacca ccaagactat ctcaggaaga atccttcagg ttactgtcat
      541 atcgatgtga ccgatgctga taagccattg attgatgcag caaactatga aaagcctagt
      601 caagaggtgt tgaaggccag tctatctgaa gagtcttatc gtgtcacaca agaagctgct
      661 acagaggctc catttaccaa tgcctatgac caaacctttg aagaggggat ttatgtagat
      721 attacgacag gtgagccact cttttttgcc aaggataagt ttgcttcagg ttgtggttgg
      781 ccaagtttta gccgtccgat ttccaaagag ttgattcatt attacaagga tctgagccat
      841 ggaatggagc gaattgaagt tcgttctcgt tcaggcagtg ctcacttggg tcatgttttc
      901 acagatggac cgcgggagtt aggcggcctc cgttactgta tcaattctgc ttctttacgc
      961 tttgtggcca aggatgagat ggaaaaagca ggatatggct atctattgcc ttacttaaac
     1021 aaataaaaca gagagtgggg cttcccactt tcttcatttc tagaatatga atagaaggga
     1081 tttatgaaac acctattatc ttacttcaaa ccctacatca aggaatcaat tttagccccc
     1141 ttgttcaagc tgttagaagc tgtttttgag ctcttggttc ccatggtgat tgctgggatt
     1201 gttgaccaat ctttacctca gggagatcaa ggtcatctct ggatgcagat tggcctgctc
     1261 cttatctttg cagtaattgg cgttttagtg gccttgatag ctcaatttta ctcagcaaag
     1321 gcagcagtag gttttgctaa ggaattgaca aacgatcttt atcgtcatat tctttccttg
     1381 ccaaaggaca gcagagaccg tctgacaact tctagtttgg ttactcgctt gacttcggat
     1441 acctaccaga ttcagactgg tatcaatcaa ttcctgcgtc tctttttacg agcgcccatt
     1501 atcgtttttg gtgccatttt tatggcttat cgaatctcag ctgagttgac tttctggttc
     1561 ttagtcatgg ttgccatttt gaccattgtc attgtagggt tatctcgatt ggtcaatcct
     1621 ctctacagta gtctcagaaa gaaaacggac caactggttc aggaaacgcg ccagcaattg
     1681 caagggatgc gggttattcg tgcttttggt caagaaaaac gagagttaca gatttttcaa
     1741 acccttaacc aagtttatgc tagattacaa gaaaagacag gtttctggtc tagtttatta
     1801 acacctctga cctatctgat tgtcaatgga actcttctcg ttattatctg gcaaggctat
     1861 atttcaattc aaggaggagt gctcagtcaa ggtgctctca ttgctcttat caattacctc
     1921 ttacagattt tggtggaatt ggtcaagcta gccatgttga taaattctct caaccagtcc
     1981 tatatctcag ccaagcgaat cgaggaggtc tttgcggaat ctcccgaaaa catccattca
     2041 gaattagaac aaaagcaagt taccagtggt cgggttttac aagtccaaga attgactttt
     2101 acctatcctg atgcggccca gccttctctg agatacattt cctttgatat gactcaagga
     2161 caaattctag gtatcatcgg gggaacgggt tctggtaagt caagcttggt gcaactctta
     2221 cttggacttt atccaataga taaggggagc attgaccttt atcgagatgg atctagtcct
     2281 cgtaatttag agcagtggcg gtcttggatt gcttatgttc cccaaaaggt cgaacttttt
     2341 aagggaacta ttcgttccaa cttgacttta ggtttaaatc aagaagtgac tgatcaagaa
     2401 ctctggcaag ccttggaaat tgctcaagct aaggattttg tcagtgataa ggaaggactc
     2461 ttggatgctc tagttgaagc aggagggcga aatttctcag gtggacaaaa acaaaggctg
     2521 tctatcgcac gagcagtctt gcgccaggct ccgtttctca tcctagatga tgcgacctca
     2581 gcactggaca ccattaccga gtccaagctc ttgaaagcta ttagagaaaa ttttccaaac
     2641 atgagcttaa ttttgatctc tcaacgaacc tcgactttac agatggctga ccagattctc
     2701 ctcttggaaa aaggtgagtt gctagctgtt ggcaagcacg atgacttgat gaaatccagt
     2761 caagtctatc gtgaaatcaa tgcatcccaa catggaaagg aggactagaa tgaaacgaca
     2821 aactgtaaac cagacgctca aacgtttagc cgtagattta gcaaaccatc ccttcctcct
     2881 tttcctagcc tttctaggaa ctattgccca agttggctta tcaatttatc tacctattct
     2941 gattgggcag gtcattgacc aagtcctagt ggctggttca tcaccagttt tttggcagat
     3001 ttttctccag atgctcttgg tggtaatagg aaatactcta gtacaatggg ccaatcctct
     3061 cctctataat cgtctaatct tctcttatac cagagattta cgagagcgaa tcatccataa
     3121 actccatcgg ttaccgattg cttttgcgga tcggcaaggc aatggagaga tggtcagtcg
     3181 tgtaaccacg gacatcgaac agttggcagc tggcttgacc atgattttta accaattttt
     3241 cattggtgtt ttgatgattt tggttagtat tctagccatg ctccaaattc atctcctcat
     3301 gactctctta gtcttgctgt tgacgccact gtccatggtg atttcacgct ttattgccaa
     3361 gagatcctat catctcttcc agaagcaaac agagacgagg ggaattcaga ctcagttgat
     3421 tgaagaatcg cttagccagc agactataat ccagtccttc aatgctcaaa cagaatttat
     3481 ccaaagattg cgtgaggctc atgacaacta ctcaggctat tctcagtcag ccatctttta
     3541 ttcttcaacg gtcaatcctt cgactcgctt tgtaaatgca ctcatttatg cccttttagc
     3601 tggagtagga gcttatcgta tcatgatggg ttcagccttg accgtcggtc gtttagtgac
     3661 ttttttgaac tatgttcagc aatacaccaa gccctttaac gatatttctt cagtgctagc
     3721 tgagttgcaa agtgctctgg cttgcgtaga gcgtatctat ggagtcttag atagccctga
     3781 agtggctgaa acaggtaagg aagtcttgac cagtgaccaa gttaagggag ctatttcctt
     3841 taaacatgtc tcttttggct accatcctga aaaaattttg attaaggact tgtctatcga
     3901 tattccagct ggtagtaaga tagccatcgt tggtccgaca ggtgctggaa aatcaactct
     3961 tatcaatctc cttatgcgtt tttatcccat tagctcggga gatatcttgc tggatgggca
     4021 atccatttat gattatacac gagcatcatt gagacagcag tttggtatgg tgcttcaaga
     4081 aacctggctc acacaaggga ccattcatga taatattgcc tttggcaatc ctgaagccag
     4141 tcgagagcaa gtaattgctg ctgccaaagc agctaatgca gactttttca ttcaacagtt
     4201 gccacaggga tacgatacca agttggaaaa tgctggagaa tctctctctg tcggccaagc
     4261 tcagctcttg accatagccc gagtctttct ggctattcca aagattctta tcttagacga
     4321 ggcaacttct tccattgata cacggacaga agtgctggta caggatacct ttgcaaaact
     4381 catgaagggc cgcacaagct tcatcattgc tcaccgtttg tcaaccattc aggatgcgga
     4441 tttaattctt gtcttagtag atggtgatat tgttgaatat ggtaaccatc aagaactcat
     4501 ggatagaaag ggtaagtatt accaaatgca aaaagctgcg gcttttagtt ctgaataagc
     4561 cattctcttt tgaaagttta tggacgaaaa aagttgcctt cgagtgactt ttttgttaca
     4621 atagctagaa aaattgttca ctgtaatact caatgaaaat caaagagcaa actaggaagc
     4681 tagccgtagg ttgctcaaag cacagctttg aggttgtaga taagactgac gaagtcagtt
     4741 caaaacactg ttttgaggtt gcatatagaa ctgacgaagt cagctcaaaa cactgttttg
     4801 aggttgcaga tagaactgac gaagtcagta accacaccta cggcaaagtg aatctgaagt
     4861 ggtttgaaga gagtacaact tgtcttttaa aaaaggagtc tataatgaaa gtctttcagc
     4921 atgtaaatat cgtgacttgt gatcaaaatt tccatgttta tcttgatgga atcttagcag
     4981 tcaaggattc tcaaatcgtc tatgtcggtc aagataagcc agcgttttta gaacaagctg
     5041 agcagattat agactatcag ggagcttgga ttatgcctgg tttggtcaat tgtcacaccc
     5101 attctgcaat gacaggtctg agagggatcc gagatgacag caatctccat gaatggctca
     5161 atgactatat ctggccagca gaatctgagt ttactcccga catgactacc aatgcggtca
     5221 aagaagccct gacagagatg ctccagtcag gaacaacaac ctttaacgat atgtataatc
     5281 ccaatggtgt ggatatccag caaatttatc aggtggtgaa aacttccaag atgcgttgtt
     5341 atttttctcc gactctcttt tcttcagaga cagaaacaac tgctgagact ataagcagaa
     5401 ctcgatccat catagacgaa atcttaaaat ataaaaatcc aaatttcaag gttatggtag
     5461 cacctcattc tccgtatagc tgcagtagag acttgctgga agcgagtttg gaaatggcaa
     5521 aagagctaaa tattccgctc catgtccatg tggcggagac caaggaggag tcaggaatta
     5581 tcctcaaacg atacggcaaa cgcccccttg cttttcttga agaactgggt tatttagatc
     5641 atccgtccgt atttgctcac ggggtcgaat taaacgagag agaaattgaa cgcttggcat
     5701 cttctcaagt ggctatcgcc cacaatccta tcagtaacct caaactggca tcaggaattg
     5761 ctccaattat ccagctccaa aaagcgggag tagcagtcgg aattgcgact gactcggttg
     5821 cttccaataa caatctagat atgtttgagg aaggaaggac tgcagctctt cttcagaaga
     5881 tgaaaagtgg ggatgccagc cagtttccaa tcgaaacagc tctcaaggta ctgacaatcg
     5941 aaggggctaa ggtccttgga atggaaaatc agataggaag tctggaagtc ggcaagcaag
     6001 cagattttct ggtcattcaa ccacaaggga aaattcatct ccaacctcag gaaaatatgc
     6061 tgtctcacct ggtttatgca gttaaatcta gtgatgtaga tgatgtttat atcgccggag
     6121 aacaggttgt taagcaaggt caagtcctga cagtagaact ttaaaagaaa aatcacgaaa
     6181 aattttaaaa aaagtttgca aaaatcttgc attctttttt tgactatgct atacttatat
     6241 acggtttaaa aaaactgcct aagacagtag gggagatcga ctcataaata tcctaccgag
     6301 gacaaaacgt atcatgtaaa aagaagcgta ttgtactttc gtgtctaggt ttgggcgcgt
     6361 ttttcttttt gaaaaattcc ccaagcaaaa taattacgga ggtgaacaca ctaatgagtg
     6421 aagcaattat tgctaaaaaa gcggaactag ttgacgtagt agctgaaaaa atgaaagctg
     6481 ctgcatctat cgtcgttgta gacgctcgtg gtttgacagt tgagcaagat acagttcttc
     6541 gtcgtgagct tcgtggaagc gaagttgagt ataaagttat taaaaactca atcttgcgtc
     6601 gtgcagctga aaaagctggt cttgaagatc ttgcatctgt atttgttgga ccatctgcag
     6661 tagcattttc taatgaagat gttatcgcac cagcgaaaat cttgaacgac ttttctaaaa
     6721 acgctgaagc acttgaaatt aaaggtggtg caatcgaagg cgctgtcgca tctaaagaag
     6781 agattcttgc acttgcaact cttccaaacc gcgaaggact tctttctatg ctcctttctg
     6841 tacttcaagc gccagtgcgc aacgttgctc ttgcagtcaa agcggttgca gaaagcaaag
     6901 aagacgcggc ttaatcttaa gctacatagc gtagcctagc tacgaaaaaa actattataa
     6961 aatttaaaac ttatttggag gaaataacaa tggcattgaa cattgaaaac attattgctg
     7021 aaattaaaga agcttcaatc cttgaattga acgaccttgt aaaagctatc gaagaagaat
     7081 ttggtgtaac tgcagctgct cctgtagctg ttgctgcagc tgatgcagct gatgctggtg
     7141 ctgctaaaga ttcattcgac gttgaattga catctgcagg cgacaaaaaa gttggcgtta
     7201 tcaaagttgt acgtgaaatc actggtcttg gtcttaaaga agctaaagaa cttgttgacg
     7261 gtgcaccagc acttgttaaa gaaggcgttg caactgcaga agctgaagaa atcaaagcta
     7321 aattggaaga agctggagct tcagttactc ttaaataata gagtatcgca atttgattgc
     7381 ggaatcccta tttaccagtc tttgagagcg ataagcgatt gatagaaact gataaattga
     7441 tttggtttcc tgccaaaaat tctttggcag gatttttgtt ttaataaact ttcgggggta
     7501 gacactagat tgtctacccc tttttttgtg tttcggaggt aattatgcaa aaggaaggac
     7561 agtcatcacg tcaattcgtg aagagtcatc tcatgactat catgggagtt gatattgtaa
     7621 aagcaactca gttagttgat gaaatggaac aagtaggttt aatccgattt gatgaatttg
     7681 gaaatgttgg aattttagtc ttggagggac aataatgaag cgtattaccg ctaatcaata
     7741 tcagacatca gagcgttatt ataagctccc caaactttta tttgagagtg aacggtataa
     7801 gaatatgaaa ctggaggtta aggtggttta ctcagtttta aaagatcggt tggagttgtc
     7861 tttgagcaaa ggttggattg atgaggatgg ggctatttat ttgatttatt ccaattcaaa
     7921 tttgatggca cttttaggct gttcaaagtc aaaattactc tccatgtgag tttgaagtga
     7981 catttttaga tgattaccat aaaaaacata actacccact attttacgaa tcctatcttc
     8041 aaaacgttat ggaattcctt gaaagtcaag acataaagaa tggggttgat gcctttgtag
     8101 atgatcatca aaatctcgtt tttgttttat atggacaagg ctatcgagcc gagggaaaag
     8161 agggaatact tacaacccaa gtaactgtaa aagcttatga tgaagacaag aaaccgatta
     8221 acttcgcaaa tttattagat tccttaattt attagattcc ttaatcgtgt cagaatatca
     8281 aatggaaccg aatctttggg aggtctccca tgattgatct ctatctaagt aaaaatagac
     8341 aaagaaatca acttctttta gatttcttcc aaaactatgg catagaggta tcttgtcatt
     8401 cagtttctga aatgacaaag gacaaattaa ttgagatgat gagctattct tcagattgtt
     8461 ttgagttttt atctcccaat ttattacgat ttaagaaccg tgacaatcta agattaacgg
     8521 atttcgttga gataatttta aaaaatcctg aactaaccat cagacttcct cttgcggttt
     8581 caaataagcg agtttatcca agtctgaatt tggaagaggc tagagctcta ttgccaagag
     8641 atacgaaaca attgatttac atggcgcaaa cacattattt atctaactaa aggagaagca
     8701 atatggctga acgattttgg gagaacttgt ccattatttt ggctgaaaga aatatcagct
     8761 ggattgagtt aaccagaaaa atgtttgcgg gagagtttca ctatccaagt gaattgaatc
     8821 gtttgtatca aaagattcgc cactagaaga tggaacaatg aatgcctcaa agtccatggg
     8881 tagaaaggat tgtgcaggta ttagatttag attatgaaga tttatttcgg aggtaactat
     8941 gaaaagaata attccagttt atatattcca acaagtaaat gtcctattgg tatctctata
     9001 cttattgaaa tttctttgta tcggtgagtt aactatacta cagattctct atggttcgtc
     9061 actcatttct tttttatgga tgtatggcca acgcaaacaa gcgcataagg tcaatatgaa
     9121 atctaggatg aaatggcttg gtgttgaatt tgttagccta ctgattataa gtctatgttt
     9181 tagtctgatt catgctcaag gaagcacgaa tcaagcaaac ttaattggcc ttcaacatca
     9241 aattccttgg ttttcatttt tattgttctt aatcaatgcg agtatggttg aagaatttct
     9301 gtatcgagaa attttatgga atttggtcag aaaattagat atcagagttg ctttgacaag
     9361 tgttttattt gccttagcac atcatacagg aaccattcta gcttggtgtt tgtatgtttc
     9421 acttgggatg tttttaggta tggtgcgcta taaatctgac ttatggggca gtatgggact
     9481 acatttggtg tggaacctac tagtttatag tttgctgctt ttttaaaata ggtgacctga
     9541 tttttaggta acttctaaaa ctaacttcca gttttgggag agagatggaa gttactttga
     9601 aaagttacgg aaacattatt ttctatgtga caacattgta taattgcttg cattttactg
     9661 gtggagagag tataatgagg gtggataaag tacttaatcc caaaaattta aataggagga
     9721 gctagctatg gcagaagaag tattaaatct tcaattagta tctgttcaag ttgacgaaac
     9781 agatgaagtt gatggaatgc gatttagtac attcagtaca aatcgttgcg gtaactggag
     9841 tgcttttagt tgggaaaatt gttaattcat ttaaattgta aactttagag attctctgga
     9901 gttatggatg tcgttccaat atgtgcacgt tggaatgtta gtgcttatat ggggagaatc
     9961 caaaggattc tcccttttta tttaaataag tctgtctagc tatagatata ttaagtttga
    10021 taaaatttct tggagatttt gttatggatt ataatttcaa tttggagcat cctttctttt
    10081 tcactaataa tgattatagt acagatacta gcataaagta ccaagtaagt ctgcctttta
    10141 attggcacga agtaatgaat aatgatgagt gggtttatca ataccctatt ggaaaatttg
    10201 ttgaaagaca agggtggaaa atacacatta gttcagagta taacagttca catgaattgt
    10261 tacaagatgt agcaaaaatt tgccatgaaa tgcgaattcc atttaaacat cttagtacag
    10321 aagacaaatt tatcatgcga aacgggaaat tggttagtcg aggattttct ggtaaattta
    10381 ttacatgtta tccaaatcaa aatgaattag agtcggtttt gcaaaggtta gaaagtgcct
    10441 taaagcaata taatgggcca tatattttaa gcgataaacg ttgggatgaa gcaccaatat
    10501 atctgaggta tggtgtgttt aggccgtcaa gggatgatga aaagaaggtt gctattgatg
    10561 aattaattgt tggcgatgaa gttgttaaag atgaacgttt accagtattt aaaattccaa
    10621 aaggtatagt gccccctgac tttttgaata aatggcttga caagaaagat aaaaaacagg
    10681 gagactttcc ctttattata gataatgcaa ttagattttc aaacagtggt gggatatata
    10741 atgctaggct aaaagaagat gggaaaaaaa taattttaaa agaggctaga ccgtatacag
    10801 gtctaggatt tgatggtact tattcttcag agaaattagc ttcggagtgc aaagcactaa
    10861 aaattctaaa tgaatggagt gagacgccta aaatatactg gcacggtaag atctgggagc
    10921 acaccttctt aggaattgaa catatgaagg gcgttccact aaatagatgg gttaccaata
    10981 attttccttt atatgaagtt gttgataaga ccaaagatta tctattgaga gtttcaaaga
    11041 tagtagaaaa actcatcgat ttaacgaata agtttcattc tgaaaatgta tatcatcaag
    11101 atttacattt aggaaatatc ttggttaagg atgaagatga gattagtata atagactggg
    11161 aacaggctgt cttttcaaat gatgaaaaag ttgtacataa agttgcagcc cctggtttta
    11221 gagcatggcg agaaacactt cccagtgaga tagattggta tggtattaga caaatagctc
    11281 attatttata catgccacta gtaactacat cggatttgac atacaactat gtaagtcaaa
    11341 ccaggataga aggaaaaaaa ctatttgagt ctttgggtta tacaagagaa catatagatt
    11401 atgtagaaag tttattgtca tatctagatt ctaaatgtcc acagattgag aatatttcca
    11461 ggaaaaaagt tttaaaatcc atgcatgaga ttagaactat cgagagtgag caagatatac
    11521 aagattttat tataaaatta ttgagagggt ttacgctaac ttatggacag tggcgtaaag
    11581 aatttcaatc aagattcttc ccagttcatt attatggtct gaattttaat caaggaattg
    11641 cgttttcaga tttagctatc ctttggagtt atcaacaact agctaagaaa gtaaaaaatt
    11701 tcaagtttga tgactattat gagattcgaa cacaggttat taatgaggca gtaaataatt
    11761 ttaagaaatc gtccctttct ggtctttttg atggtaaaat tggtacaatc tggctcattt
    11821 atgaatttgg ggagatagat cgggcagttg aactttttac aactcatttt atagagatat
    11881 ttgagaattc acaaaataaa aatttatata gtgggcaggc tggaatttta ctggttggac
    11941 tgtattttct tagtaaaggg gaaattgata ataaactagg agaagaaata ctaattaggc
    12001 ttagagaata tactttaaat tatatagaaa atccagaaac tttttgtaaa gttggcgcta
    12061 gtgatgttca gagtaatgat ccttatgaga attttggtgg attactatat ggtcacgcag
    12121 gggtagcttg gctcttcggt gaagcttata aattaactgg tgaaagtatc tataaaaatg
    12181 gtctagaatt ggctgtagat aaagaattag ttgcttataa agtagatagt aataattcat
    12241 tgcagtacag ccaaggtcat agattactac catatctagc aactggaagt gctggcctgc
    12301 ttttacttat taatagaaat aaagaaatct tgtcaagcaa atacttaaaa tatttaactt
    12361 ctttagagag ggcaactgat gtagtttttt gtgtattacc aggtttattt aacggatttt
    12421 gtggtcttga agttgctaat aatatatata gtgatatcga tgataacttt tcaggacaaa
    12481 agaaattaat tgaacaactt tatagatatt tgtgtgtaat tgaagaagga tttgttattg
    12541 cgggagataa tggtttaaag ataacaacag atattgcttc gggatttgcg ggtgttgcaa
    12601 ttggattggt atcaataatg gataataaat tgacgattct tcctcaaata taatatttag
    12661 ataccattat taaggggtca tacttgtgaa tcagagtaat gaaatatttt taaatacaat
    12721 acaggaaaaa acacactata agaggtctat ctcttcatca tttcaggaat taaaaaaatg
    12781 gaattctttg atatttagga gagggcgata ttggtaccaa tttgataatt ggagtatacc
    12841 caatactggt gtaataaagg aatggtatgt cgaagataat gactggattc ctaaggttag
    12901 ttcttccagt ttagacaatt caaattcact taaaattgtc tttgatgtgg aaaaatatac
    12961 tttgccagat gatagtatct tacaggatgt taggatttca tctgatgaga gttatatagt
    13021 tttggttgta tcctcactgc gtagcactaa tttaattgga ataaaaaaat atactatgga
    13081 agagttattt atcattgaag atatttcagt agaaagttcg ttctatttgg gtaaattcgg
    13141 tgttatgtat actcgctcca aagagtacgg gaggccaagt aagttattct ataaatcttt
    13201 tgatagcttt acagaagagg aactgtttga agaaaacgaa tgctcttttc gattaaaaat
    13261 agtccacatt gatagcaata attgttttgt aaagtcagta gactttcaga aaggcaggat
    13321 ttttctatac agtttcgacc ggacgggttt tgttagacac tcttacacag aaacggtagc
    13381 cccaacacct agagatatcg ctctatttag tacagaaaaa gcacagtact ttcttgggat
    13441 ctcttcaact gaagaaaaga aagatcaact aattctaagg gaaacttctt cgggagacag
    13501 agtttctatt actataccat atcaagatag ggctagaaga gttcactgta taggacgtta
    13561 tatcctttta gactgttcaa acgcagcgaa ttccgtcttt tatcttgcta catttaaaaa
    13621 taatagtttg agggagttga gtgttaataa gataacattt gatgaaaaga caacacttta
    13681 tgaaaattca ttttctgatc gtgttcttct gtttttagag aggagaactt tttacgaaaa
    13741 aatacttagt tttgatttaa tagaaaatac attaaaaact gaatttgaaa gacccatcat
    13801 aaaatcgaat aatactaaat atttttctaa ggtgatttgg actaaaaaag acagttatga
    13861 tgtaagcata ccgatttctt tattttggaa aagtgaagat acggatgagc tcccaagaag
    13921 aaaaaaatgt attctaagtg tctatggggc ttatggtaag aatgataatt ctgatttaga
    13981 tgaaattatg ttatctataa tagatgcagg ttttatttat gctatagttc atgtaagagg
    14041 tggaggctat ttgggtggtg aatggtatcg ctcaggaaag gcgttaaaca aatggaattc
    14101 tatcagagat tttattgagg gagtaaatta tttaagggaa aatgatgtaa ttgacagtaa
    14161 gcgattaggt ttaataactt ctagtgcagg tggaataatt gctggtgcgg tgttgaacga
    14221 ggaaaaaaat ttattgcaaa gcattctctt attttcgcca tttataaatc cttatgatac
    14281 acttcagaat ccaaatgatc ctctttctaa gaccgaaata gcagagtggg gagatattag
    14341 agcccctgaa gtaaaggctt atataaagtc atattcgcct atgcaaaata ttgaaaaggc
    14401 acgagactca aatactgtta tagttaatat tttgggtgag aaagacccat atattaataa
    14461 taacgaagtg atagagtggt caaagaaatt aaattctatt ggagttaaaa gtttgttgta
    14521 tttaaacaaa gcagctgatc atggaggttt tactc
//
LOCUS       DAVKFH010000039        14268 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKFH010000039 DAVKFH010000000
VERSION     DAVKFH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.039"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..1062
                     /locus_tag="VW006_001419"
                     /pseudo
     CDS             1..1062
                     /locus_tag="VW006_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1147..2349)
                     /gene="rpsA"
                     /locus_tag="VW006_001420"
     CDS             complement(1147..2349)
                     /gene="rpsA"
                     /locus_tag="VW006_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9380254.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2511..2582)
                     /locus_tag="VW006_001421"
     tRNA            complement(2511..2582)
                     /locus_tag="VW006_001421"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2548..2550),aa:Gln,seq:ttg)
     gene            complement(2592..2672)
                     /locus_tag="VW006_001422"
     tRNA            complement(2592..2672)
                     /locus_tag="VW006_001422"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2636..2638),aa:Tyr,seq:gta)
     gene            complement(2722..2952)
                     /locus_tag="VW006_001423"
     CDS             complement(2722..2952)
                     /locus_tag="VW006_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9380255.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(3120..3764)
                     /gene="pcp"
                     /locus_tag="VW006_001424"
     CDS             complement(3120..3764)
                     /gene="pcp"
                     /locus_tag="VW006_001424"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9380256.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3779..4702)
                     /locus_tag="VW006_001425"
     CDS             complement(3779..4702)
                     /locus_tag="VW006_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9380257.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4699..5388)
                     /locus_tag="VW006_001426"
     CDS             complement(4699..5388)
                     /locus_tag="VW006_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9380258.1"
                     /translation="MEWIRLIGIAIIVVGFIFKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5453..6157)
                     /locus_tag="VW006_001427"
     CDS             complement(5453..6157)
                     /locus_tag="VW006_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9380259.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(6213..7235)
                     /locus_tag="VW006_001428"
     CDS             complement(6213..7235)
                     /locus_tag="VW006_001428"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9380260.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7367..9838)
                     /gene="parC"
                     /locus_tag="VW006_001429"
     CDS             complement(7367..9838)
                     /gene="parC"
                     /locus_tag="VW006_001429"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9380261.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(10258..12201)
                     /gene="parE"
                     /locus_tag="VW006_001430"
     CDS             complement(10258..12201)
                     /gene="parE"
                     /locus_tag="VW006_001430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9380262.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGFKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAVVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            12337..12978
                     /gene="plsY"
                     /locus_tag="VW006_001431"
     CDS             12337..12978
                     /gene="plsY"
                     /locus_tag="VW006_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9380263.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(13021..14259)
                     /locus_tag="VW006_001432"
                     /pseudo
     CDS             complement(13021..14259)
                     /locus_tag="VW006_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4107 a         3189 c         2492 g         4480 t
ORIGIN      
        1 agtgctatca ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag aagaatcacc
       61 aaatccctcg tatcatcaac caaaagattg ctcaaaagtt aattgaaaag atttctatga
      121 ccgatattgc ccatcagctt tccatctcaa cttcaactgt cattcgaaag ctcaatgact
      181 tccactttga gtgtaatttt agaaatctgc ctgagattat gtcttgggac gttgaaacag
      241 tccggggagt gactgtttca atcgggagat ggagatgagc tttattgcgc aagattttga
      301 aaagctcaat atcatcactg ttcttgaagg tagaacacaa gctatcatcc gagatcactt
      361 tcttaaatat gatagagccg ttcgttgtca agtgaaaatc attactatgg atatgtttag
      421 tccttactat gacttagcta gacaacttcg ctttcaaatt tctaggctca ggctgaaaca
      481 gtctctcaga ctgtttcact cccgaatgct aaaatcgttc ttgatcgctt tcatattgta
      541 caacatctta gccgtgctat gagtcgtgtg catgtccaaa tcatgaatca gtttcatcga
      601 aagtctcatg aatacaaggc tatcaagcgc tactggaaac tcattcaaca ggatagccgt
      661 aaactgagtg ataagcgatt ttatcgttct acttttcgta tgtacttaac caataaagag
      721 attttaaaca agcttttgag ctattcagaa gacttgaaac accactatca gctctataat
      781 aaggaaccag agaaattttt cggacttatt gaggacaatc ttaagcaggt tcatcctctt
      841 tttcagactg tctttaaaac ctttctaaag aacaaagaga aaatcgtcaa cgcccttcaa
      901 ctaccctatt caaacgccaa attggaagcg accaataatc tcatcaaact tatcaaacgc
      961 aatgcctttg gttttcggaa ctttgaaaac ttcaaaaaac ggatttttat cgctctgaac
     1021 atcaaaaaag aaaggacgaa atttgtcctt tctcaacctt agctgacttc aacccactac
     1081 agttgacaaa gagccagaaa aatcccttgt ttagtaaaca aaggattttg tgaattttca
     1141 atttgattaa agttcgatat caccaaacaa atcagccatt gaaaatcctg tttgtgtttc
     1201 tggaagttcg aaatcacgct tttcttgacg tcttggacga cgtggacgag cagcacgttt
     1261 ttcttctttt tgtccttctt cttgggctgg acgttcttca agagctttaa tagaaagtga
     1321 tacgcgttct gcatctgcgt taacttcaag aactttaact tgaacttctt gaccaacttt
     1381 aagagcttct tttggatttt caatccgttt gtgtgaaatt tgtgatacgt gaacaagtcc
     1441 atcgatacct ggcaatactt caacaaatgc accgaagtca gtcaaacgtt taactgttcc
     1501 ttctactaca tcacctttag ccaatttttg ctcaacgcca tcccatggtc ctggtactgt
     1561 tgctttaagt gaaagtgata cacgtccttc ttcttcgtta agatcaagga ttttcacttc
     1621 aatttcttca ccaacagtta caactgattt tggtgatacg ttacgttcat gtgacaattc
     1681 agtcaagtga accaatccgt caacaccacc aaggtcgatg aaagcgccga agcttgtgat
     1741 acgagcaact ttaccagtta caacatcacc aacagccaat ttaccgaata cttcagcgcg
     1801 agctgctgca gtagctgctt caacaacttc acgacgtgaa aggatgaagc ggttttcttt
     1861 agcgttaact tctttgattt tagtatcaaa ttcttgacct acaaaacgct cagcgttacg
     1921 tacgaaacga gtatccaaca ttgaagctgg gataaatcca cgaacacctt caaattctac
     1981 tgaaagtcca cctttaacgg cacgcgttcc tttaacagta acaacttctt cttcgcgacc
     2041 aacaagtttg tcccatgctt tgcgagcttc aaggcgtttt ttagatacaa ggtatgtaac
     2101 tgtatcagta tctttaccaa ctacttgacg aagtacaaga acatccaata cttctcctac
     2161 tttaacaaag tcattgatat ctgcatcacg atcgtttgtc aattcgcgaa gagtcaagac
     2221 accttcaaca ccagttccag agattgcaac gttagcttga gtcgcatcaa ctgtcaatac
     2281 ttcagcacta acaacatcac cagtctcaac ttgactaacg ctatttagca aatcttcaaa
     2341 ttcgttcatc taaaaaatcc tccaacaatc aagtttttct taaacttgac atacttataa
     2401 tttttttcct aagcacccgc aaggacatgt tcatatcatt cacgtctttc aattataaaa
     2461 ataaaatacc ctaagatatt ttgcttttta tcttgattat tacctaagaa ctggggtagc
     2521 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
     2581 cattaatgaa atggagagag agggattcga acccccgaac ccgaaggagc ggatttacag
     2641 tccgccgcgt ttagcctctt cgctatctct cctacaatca acatggacta ttatatcatg
     2701 aaaatttcaa aaaaacaaga cttattttgc taaattataa ttttcaatca aaatttccac
     2761 agctttttcc aattttttat aaaaactatc gacattccca ttctttataa tggcaaattg
     2821 tccgtctaat tcggcaattt ctccgataac ttttttaccg atagtcaatg tataaccttc
     2881 aaaactgtct ttaccaacat taactttggc atccgctact tgaatttcga tttttttatc
     2941 tttcttgctc atttcatacc tctcttcact ttttctattt tacaagaaaa tcctaaaact
     3001 agcaagaaaa aactctatat caggcttctc tgatatagag ttttatgtaa acaacctcaa
     3061 tcgatttagc atagctaata gttagtaaac aagcataatt gactatcctt tttaggttat
     3121 cagtgagtct caccgcctac taactgcaaa tccttatctt tgtagtctac aatagcttca
     3181 atagctacct ctatgcctct tacaatatca catagactca ttcctgcagt attcggctta
     3241 tttactactt gttccatcat atatggaata tgcatgaaac ctgctctcat attagggaat
     3301 tttttatcca ctaaataaag agcttggtac atcaaatgat tgcaaacaaa ggttcctgca
     3361 ctattggata caactgccgg aagtccctgt tttttgatag cttgtaccat cgctttgata
     3421 ggtaaactac taaaataggc cgatgctcca tcaatacgaa tcggtgtatc aattggttga
     3481 ttgccttcgt tatcaggtat gcgagcatca tcttgattaa tagccactcg ttcaggtgtt
     3541 aagccggtcc tgccgcctgc ttgtccaata caaagtacag catctggttg atatcgtaat
     3601 atttctgcct ctaaaacttc tgacgactta taaaaaaccg ttggaatttc tacccagcga
     3661 acttcagccc cattaatctc agatggtaat aattttacag cctccaaagc tggattaatc
     3721 ttttcacctc caaaaggatt aaaacctgta accaatattt tcattttatt ttcctttact
     3781 aaaatgcgag aaagtacatt aagaatatgt gaataacaat cattactaga gcaacacctg
     3841 cttgagcctt tataacgcca ttctgatctt tcatatccat caatgctgct ggtagagcgt
     3901 taaaattagc agccattggg gtcaataagg tcccacaata acctgctgtc atggcaagag
     3961 caccagccac aattggatta gctcccagag caaatacaaa gggaactcca acacctgctg
     4021 taataacggt gaatgctgca aaagcatttc ccataatcat tgtgaataga accattccaa
     4081 gaacataggc caaaactcct ataaagcgac tatctgaagg aacaataccg ctaatcagat
     4141 gagagataac atcaccaaca cctgctacag taaaaatagc ccccaaagcc cctaataatt
     4201 gaggaacaat accacttgtt gaaacttgct gagtcattcg attattttct gataacagac
     4261 tcttagggtg actattggta atcacaagaa cagaaattgt agcaaacaag gcggcaaggc
     4321 taatcgaaat cttgctaaat tctggaatca tttgcgctaa gaccaacgca agtattgcca
     4381 tcagcataac tggaataaaa attttatttt tcaacctgtt agattcaata ttggctttca
     4441 tttcatctaa ggatggcaag gttccgatac ggacttgctt aaacaatgtt aacagcgata
     4501 ataggattac aataatacca atactcatat ttggcatata ggaaccacct ataaacgtaa
     4561 tagacaatag agtccaaaat gcagatgtcc caagtcgaac tgggtttgtt ttatctttat
     4621 aactacaata ggctgtatgg agaaattgac aaccaatcac aatataggtc aactctaata
     4681 gttgctttgc caactctgtc atttttgttc tcctccccta gtcttttttg atatcaattt
     4741 tttatcaaat aaataattgt aaatccccac tacaataagt gttataacag caacaataat
     4801 agatgtagaa gcaatccctg cataattgct ttcatagcct aactgatcta atgttccccc
     4861 tatcaagagg actcccccag cacctacaaa cgtattttga gcaaagaaat ttccaaaatt
     4921 ttcattcgca gccgcacgcg cttttattgt ctcatcttca acctctgtta actttctacc
     4981 taattgagac tctgcagctg cttctcccat aggttgaacc aaaggtctga caaactgagg
     5041 gtgtcctcct agacgaattg aaaagaaacc agctaactct cgaataaaaa aataaactgt
     5101 atagaagttt ccaactgtca gacctttaat ctttcgaatc aaatcgattg atcgttgctt
     5161 gagtccaaag gtttctgaca gccccacaag aggcaaggta accataaaaa tcgtgagcac
     5221 tcgctgattg ctaaattctt ttcccaaaat ctccaaaaat tcaacgagag aaacacctga
     5281 aactaaagct gtaaccaaac cagctaagac tactgttgca attgtatcaa atttaaaaat
     5341 aaaacccaca acaatgattg ctattcctat taatctaatc cactccatat caaactcctt
     5401 tatattcaaa atgacagtat tttaaaaatt ttatcaagat caataccatt ccttatttaa
     5461 tgtatttttc tagttctttt tggtatttgc tattggattc caatttttct ttttgccatt
     5521 ttttaaaaac ctcgttatat tcttttgttg taacaatatc tttttgcaat ttcattcctt
     5581 taaagatata tggttccccc ttaataccaa cttgtgagta tggttttgag aatggtacta
     5641 cgttacttac aactggagaa ccaccagatg aagctgttgg catcaataat gaactatctg
     5701 tcgaccaagc ttgagctttg gcatattttt catatctctt ctctaggtca gtggtctcag
     5761 aaacagcatc ttctaacaat ttcttatatt tatccaaacc aggtttagct acaacatcct
     5821 tatcttttcc tttcgtaata ccaaggtgtt tcatggcaga accagatttt ggatccataa
     5881 tattcaagta agacgctgga tcttgatagc ttggagccca tcctgtacta ttcaaatcat
     5941 agtctttttg agaaggagca acattgccgt atttatcatt ttccatcaaa ccatcaataa
     6001 catttccaat aacgtctgtc ctcgatgttc gagtcgctat actgtagccc aatgatgctg
     6061 gatctactgc atagacataa gaaaatgttg tcggtgcatc tgcttcttta tcagtttttc
     6121 cacaagccac taaaatagct gacgtgctca ggaccactcc tgctgttaag agccactttt
     6181 tctatttcat aaaaaatctc ctttggttta ttttaatcta cttttacaat ccaaccttct
     6241 ggcgcttcaa tatcgccaaa ctgaataccc gtcaattcat tatataattt acgcgtcaca
     6301 ggacctactt ctgtttcact atagaataca tggaaatcat caccatgttg aatacctcca
     6361 attggagaaa taaccgctgc tgtaccacag gcacctgcct ctacaaaacg gtcaagatta
     6421 tcaattggaa catcaccctc aataggagtt aatcccaagc gatgttctgc caaataaagc
     6481 aaggaatact tggtaataga tggcaagata gatggactca atggtgttac aaattcatta
     6541 tcagctgtaa ttccaaagaa gttagctgat ccgacttctt caatctttgt atgagttgat
     6601 gggtccagat agataacatc tgagaaatga cgtgacttgg ccatttttcc tggtaagaga
     6661 cttgcagcat agtttccacc aaccttagcc gcacctgtac catttggtgc tgcacggtcg
     6721 tactcatcct gaatcaagaa gttggttggg accaaaccac ctttaaagta atttccaact
     6781 ggcatagcaa agatggtgaa aatgtactct tctgccggtt ttaccccgat aatatctccg
     6841 acaccaatca aaagagggcg aagatataag gttccacctg ttccgtatgg tggtacgtat
     6901 tcttcattcg cacggacaac tgctttacaa gcttctacaa acatgtctgt cggaacttgt
     6961 ggcatcaaga gacggtcaca tgtacgttgc agacgtttag cattttcatc aggacggaac
     7021 agttgaacac tgccatcctt agtacgataa gctttcaaac cttcaaatgc ttgttgtcca
     7081 tagtgaagac ttggagaaga ctctgaaata tgcaaagttg catcctctgt aagctctcct
     7141 tgatcccatt gtccattttt gaaatgagca agatagcgat aaggtaattt catataggaa
     7201 aaaccgaggt tttcccaatc aatcgttact gtcatattcc actccttatt ctaaaaacct
     7261 atttcttata ttctacacta tttttctaaa atagcaagta tattttgtaa ttttcagaaa
     7321 atttctccaa taaaaaccag ctcttagaac tgattcttca tttcacttat ttatcttcag
     7381 taactacttc ctgaagataa gcgtcaaaaa cttcttcatc tgaaatcgtg tcagaaatga
     7441 agcttccatt gctagtgcgt tctgacaagt tcaagtcttg caatcggctt tcatagattg
     7501 ttcctttatt ggattggaca agcagagttt ggtcgttcac atccacttcc gtactgaaga
     7561 aatcgccaac aaatccttgc tctgcaactg ctcctgccaa gaagacacga tgcggtttgt
     7621 ttttcaactc acgcaagact tgtaatcctc gtttggcacg gctggttgct agaatttcct
     7681 caatggaaac acgtttcaag cttccacgct gggtcaagag gtagaaggac gaagtattac
     7741 agataaagcc agattggagg acatcatctt ctttcaaatt catagccttg acacctgctg
     7801 ccttagcacc gacaaccgga acctcttcga tattgaaacg cagggcataa ccattttgac
     7861 taaccaagac aacatcatct agtttaatcg gagccactgc tacaatctga tctgtatcgt
     7921 ctttgagctt agcatacttg acagacttag atctataggt ccgccatgga gtgaattctt
     7981 ttcgctctac ccgtttgatt tgaccaaggc gagtcactgc aaagtaggtt gtcgcatcgt
     8041 caaactgatc cagtacttcc acataaagga tttcttcatt cgtttcaaag tttgtgatgg
     8101 tttggctcag atgctctccg atgtccttcc aacgaatatc tgccaactca tggattggtc
     8161 tgtagatgac atttccaaga cttgtgaaca tcaagaggtg ctgggttgtc ttggcagatt
     8221 gaacaaaaat caaacggtca tcatcacgct tgccaatttc ttccaaggtg gaagccgcaa
     8281 aggaacgtgg actggtacgc ttgatgtaac ctgccttggt cacgctgacg taggtatctt
     8341 cctcagcgat aagactagct gtatcaatct caattgcttt cgcagtgtct tctaaagaac
     8401 tcaaacgagg agttgcaaat ttcttcttga cctcacgaag ttctttcttc atgagattgt
     8461 acatagtcct ttcatcaccg ataatagccg ccagcatagc aatcttctca cgaagctctg
     8521 cttcttcttc ctgcaagaca accacatcgg tattggtcaa acggtacagt tgcaaagtta
     8581 cgatagcctc agcctgttct tccgtaaaat catagctaac tttgaggttt tccttggcgt
     8641 ccgccttatt ctcagaagca cggataagag caatgacttc atccaaaatc gaaatcacac
     8701 gaatcaaacc ttcgacgata tggagacgtt tctcagcctt ttctttgtca aagcgtgaac
     8761 gcgccaaaat cacttctcga cggtgagcga tgtagctaga caggattgga acaatcccaa
     8821 cctgacgagg tgtgaaattg tcaatcgcca ccatattaaa gttgtagttg atttgtaggt
     8881 cggtgtactt aaataagtag ttgagaacaa gctcagtatt agcgtctttc ttaagttcga
     8941 tagcgatacg aagaccatca cggtcagact catcacgaac ctcagcaatc ccagctacct
     9001 tgttattaac acgaacatca tcgattttct tgactagatt ggccttattg atttcataag
     9061 gaatctcagt aataacgatt tgttccttac caccttttag cttttcaatt tcagtcttgg
     9121 aacgaacaac cacgcgccct ttcccagttt cataagcttt cttgatttca tcacgaccct
     9181 gaataatagc ccctgtaggg aagtctggtc caggcaagaa ttccatgagt ttatcaatct
     9241 ttgcagttgg gtggtcaatc atgtaaactg cagcatctat gacctcagct aaattatggg
     9301 gaggaatgtc tgtggcataa ccagccgaaa tcccagtcga accattgacc aagaggtttg
     9361 gaaaggctgc tggcaagacc gttggttctt tctccgtatc gtcaaagttc catgcaaaag
     9421 gaactgtatt tttctcgata tcctgaagaa ggtaacctgc aatttcagac aaacgtgcct
     9481 cagtataacg catagccgca ggaggatctc cgtccataga accgttatta ccgtgcattt
     9541 caactagaat ctcacgattt ttccagttct gtgacatacg aaccatggca tcatagatag
     9601 aagaatcccc gtgtgggtgg aaattcccca tgatgttccc gactgacttg gccgacttac
     9661 ggtagctctt gtcaaaagta ttgctatcct tattcataga ataaagaata cggcgctgaa
     9721 ccggcttcaa cccatcacga atatctggca aagcccggtc ttgaataatg tacttggagt
     9781 agcgaccaaa gcgctctccc atgatgtcct ccagggacat gttttgaatg ttagacataa
     9841 gatacaaagc ccataaaata ccaagtgaaa atagaaaatt cttgaagtaa gcaaactcac
     9901 aagagaattt atctttttca cacagtatct agggcgtgtt caactccttt caaagaatgt
     9961 agagtaggtt tttatgcagt aaaagatatt ttacgggaat tcctcccgtg ttcagttacg
    10021 ataagtaacc aaactatcct gtttgtattt ttcaatatga aaatctggtt ttccaaaatt
    10081 agtcttagtt tgtgtcttag ccgctccctt aagcgcctct ttgagataag cactcatagc
    10141 agattcttca ttaataatcc tgcaattttt tcaaaccaag attttcaaac tgctttttca
    10201 catagtcatt cacatccgac tctaatttcc agtttactaa catattattt tctttcatta
    10261 aaacactgtc gcttcttcta gcgtaaactt gacattatct tcaatccatt tacggcgtgg
    10321 ttctacctta tctcccatga gaacattgac gcggcgttcg gcgcgcgcta aatcttcaat
    10381 tgtgacacgg atgagggtac gtgtttctgg gttcatggtt gtttcccaga gctggtccgc
    10441 attcatctca ccaagtcctt tgtatcgttg gagggtagcg cctttaccga actgtttacg
    10501 gagttcttct agttctccgt ccgtccaagc gtaggccact tcttctttct tgcctttacc
    10561 tttggacatc ttataaagag gtgggagggc aatatagaca tgacctgcct cgactagcgg
    10621 acgcatgtaa cggtagaaaa atgtcaagag caaggtctgg atatgggcac cgtcggtatc
    10681 cgcatcggtc atgataatga tcttatcata gttggcatct tcaatagaga agtctgctcc
    10741 aacacccgca ccaatggtat aaatcatggt attaatctct tcatttttga ggatatccgc
    10801 catcttggcc ttggctgtat tgacaacctt accacgaaga ggtagaatag cctggaactt
    10861 gcggtcacga ccttgtttgg cagaaccacc ggcagagtcc ccctcaacta gatagagttc
    10921 attcttagca ggattcttag attgggctgg ggtcaatttc ccagacaaca agcccttatc
    10981 tttcttgttt ttcttcccat ttcggctctc atcacgcgcc ttacgtgctg cttcacgagc
    11041 atcacgggcc ttgatagcct tgcggatgag gttagaagct aattccccat tttccataag
    11101 gaaaaaggtc aacttatcag ccactattcc atccacaact gggcgagcta gggggcttcc
    11161 tagtttatcc ttggtctgtc cttcaaactg caagtgttct tcaggaacta agatagaaag
    11221 aacgaccgct agtccctcac gatagtctga accttcaagg tttttatctt tttccttgag
    11281 aagacctgtt ttacgtgcat agtcattcat gaccttggta atggcagact tgaatcctgt
    11341 ctcgtgcgtt ccaccgtcct tggtgcgaac gttattgaca aaggatagaa tgttatctga
    11401 gaatccgtca ttgtactgga gggctacttc cacttgaaaa ccattgtctt ccccttcaaa
    11461 gtaaagaact ggcgtcaaga tttccttatc ttcgttgaga taagaaacaa aatcttgtac
    11521 tccattctca tagtggaact caatcgcttc atctgttcgc ttgtccgtta aagacaaggt
    11581 cacatttttc aagagaaagg ctgattcatt aaggcgctct gaaatggtat tgtacttgaa
    11641 atctgtcgta gaaaagatag tcgcgtcagg cataaaagta actttggtgc ctgttttaga
    11701 cttgggtgct gtaccgattt tcttcaaagt cgtgacaggt tttccaccat tttcaaaacg
    11761 ttgcttgtaa actgcgccat cacgggtaat ttcaacttct aaccagctag aaagggcgtt
    11821 aacaacggaa gaacccactc cgtgaagtcc acctgatgtc ttatagccac cttgaccgaa
    11881 tttccctccg gcatgaagaa tggtaaagat aacctcaaca gttggaattc ccatagcgtg
    11941 catacctgtc ggcatcccac gtccatggtc ttgaaccgtt agactaccgt ctttattgat
    12001 agttacatca atacgatcac caaacccaga caaggcttca tcgactgcat tatcaacgat
    12061 ttcccaaact aggtgatgaa gaccagcgcc atcggtcgat ccaatataca tccctggacg
    12121 ttttcggacc gcatccaacc cttctagcac ctgaatagca tcatcattat aattgttaat
    12181 attgatttcc ttttttgaca caaggaacct cctattcgtt catctttact attctacagg
    12241 ttttccaagg attttgcaaa atttttcttt ctccgatgtg acaatttcag cagagattct
    12301 ctgcttttct ttcccaattc atgatataat aggagtatga ttacaatagt tttattaatc
    12361 ctagcctatc tgctgggttc gattccatct ggtctctgga ttggacaagt attctttcaa
    12421 atcaatctac gcgagcatgg ttctggtaac actggaacga ccaacacctt ccgcatttta
    12481 ggtaagaaag ctggtatggc aacctttgtg attgactttt tcaaaggaac cctagcaacg
    12541 ctgcttccga ttatttttca tctacaaggc gtttctcctc tcatctttgg acttttggct
    12601 gttatcggcc ataccttccc tatctttgca ggatttaaag gtggtaaggc tgtcgcaacc
    12661 agtgctggag tgattttcgg atttgcgcct atcttctgtc tctaccttgc gattatcttc
    12721 tttggagctc tctatcttgg cagtatgatt tcactgtcta gtgtcacagc atcgatcgcg
    12781 gctgttatcg gggttctgct ctttccactt tttggtttta tcctgagtaa ctatgactct
    12841 ctcttcatcg ctattatctt agcacttgct agtttgatta tcattcgtca taaggacaat
    12901 atagctcgta tcaaaaataa aactgaaaat ttggtccctt ggggattgaa cctaacccat
    12961 caagatccta aaaaataaaa cgccagttct gtactgaccc caaaaagtta gacaattaat
    13021 ttatccgaag gatttagttc tgtattgcac agggctaagt ccttttagtt ttaccttaat
    13081 tcgtttattg ttgtagtaat caatatagtc tacaatggct tgttccaatt gcttaagcga
    13141 ctgaaacgac ttctcataac cgtaaaacat ttccgatttc agaatcccaa agaaggactc
    13201 catcatacca ttgtctgggc tgttaccctt acgtgacatg gatgcttgaa ttcccttacc
    13261 ctcaagaaac tgatgataag aatcgtgttg atattgccag ccttggtcac tatggagaat
    13321 cgtattctcg tagtgcttct ctgtgaatgc ctgttccaac attgtttgta cttgttctaa
    13381 gttgggtgaa gttgaaagat tataggcgat aatttcgctg ttaaaaccat ctaaaactgg
    13441 tgataagtaa agcttttgag tacttactgg aatggcaaat tcagtcacat ctgtgtagca
    13501 ctgttccatt gttttagagc cttcaaattg gccttgaatg agattctctg ctttcttacc
    13561 aacgtctcct ttatgagaag aatattttcg tttctgtcgc attttagctt gtaaattgag
    13621 tactttcatc aagccttgaa ctcttttatg atttaccaga taaccacgat ttcttagttc
    13681 taaataaatc cgacgataag cataatttcc cttgtgttcg ataaaaatgg attgaatttc
    13741 agctttaagc tcttggtcct tatctggttt gtctagctgt ttcaagtgat agtagtaggt
    13801 cgaacgagct agtttaatgg cttttagaag aatatctaac gaaaactcag tcattaattc
    13861 ttgaacaatt tctgtctttc ttctttctct ttttcctcct tcaatcggag ttctcttaac
    13921 ttttttagga tggcattctc cgctctcagg tactcatttt ctgcttgaag atgttctaat
    13981 tctgtcctct cttcaggtct cttttttggc ttacgtccca ttttaggtac tctccctctt
    14041 gttttctcaa caatagtata cccgtttttt tctgtattgt gctagccagt taagaagtat
    14101 cgtacgactt gggagaccgt attcaagaga aactctgtct ttagtccagc cttcatgtca
    14161 gactttatta atcatttctt gttttaaatc aggagaatag taacgatttt ttcctttttt
    14221 gacgaactct attccgtaac gatcaatcaa tttaatcatg tacctaat
//
LOCUS       DAVKFH010000040        14250 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKFH010000040 DAVKFH010000000
VERSION     DAVKFH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.040"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(778..1647)
                     /locus_tag="VW006_001433"
     CDS             complement(778..1647)
                     /locus_tag="VW006_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9380264.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1871..2392)
                     /locus_tag="VW006_001434"
     CDS             complement(1871..2392)
                     /locus_tag="VW006_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9380265.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(2350..3456)
                     /locus_tag="VW006_001435"
     CDS             complement(2350..3456)
                     /locus_tag="VW006_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9380266.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVVVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3567..3821)
                     /locus_tag="VW006_001436"
     CDS             complement(3567..3821)
                     /locus_tag="VW006_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380267.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(4161..4694)
                     /locus_tag="VW006_001437"
     CDS             complement(4161..4694)
                     /locus_tag="VW006_001437"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9380268.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     RAPRGEKVYKKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(4681..4896)
                     /locus_tag="VW006_001438"
     CDS             complement(4681..4896)
                     /locus_tag="VW006_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9380269.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCALPSVWAALKYIKVTLKKDDEL"
     gene            complement(4863..5006)
                     /locus_tag="VW006_001439"
     CDS             complement(4863..5006)
                     /locus_tag="VW006_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU9380270.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            5240..6958
                     /locus_tag="VW006_001440"
     CDS             5240..6958
                     /locus_tag="VW006_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9380271.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            7069..7416
                     /locus_tag="VW006_001441"
     CDS             7069..7416
                     /locus_tag="VW006_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9380272.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7695..8531)
                     /locus_tag="VW006_001442"
     CDS             complement(7695..8531)
                     /locus_tag="VW006_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9380273.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8544..9173)
                     /locus_tag="VW006_001443"
     CDS             complement(8544..9173)
                     /locus_tag="VW006_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9380274.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9183..9824)
                     /locus_tag="VW006_001444"
     CDS             complement(9183..9824)
                     /locus_tag="VW006_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9380275.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(9970..11199)
                     /locus_tag="VW006_001445"
     CDS             complement(9970..11199)
                     /locus_tag="VW006_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9380276.1"
                     /translation="MNNSQQMLQALEEQDLTKAEYYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLMLGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11189..12355)
                     /locus_tag="VW006_001446"
     CDS             complement(11189..12355)
                     /locus_tag="VW006_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9380277.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVIFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12499..13512
                     /locus_tag="VW006_001447"
     CDS             12499..13512
                     /locus_tag="VW006_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9380278.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNEFHVPEAVCIR
                     FAP"
     gene            13652..>14250
                     /locus_tag="VW006_001448"
     CDS             13652..>14250
                     /locus_tag="VW006_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9380279.1"
                     /translation="MEQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYA
                     FTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHFLHYDRA"
BASE COUNT         4369 a         3052 c         2497 g         4332 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatac ccccctaatc
      361 gcttatttta tagcaaaatg aaatagaaac tgaacatttt gttgaggaag gtaaatcaat
      421 tcctcacaat gtttgagaaa gaagggagga ctattccaac ttcaacccac tataaagcag
      481 acttacaaga tagggaaccc atctccgata atatatagta gaatgaaaac tgcacaattt
      541 gattggagaa ttcaaaccaa tttctcacaa tgttttagaa acaagggtct attatttcaa
      601 ctgcaaccta ctatagtcca ttgaagctgg aatagcccac tgagacttct aaaccactgc
      661 tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa gagagaacca
      721 gtctgatcct ctcttattta tagatggtgc tcactcaaat gttaaaggag ctatttctta
      781 gagataaagg aatttgaata cagctactgc cgcaattgct gctacgatag gtgctactac
      841 tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa ctgattttgg
      901 aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg caggtcctgt
      961 aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca agtgagccac
     1021 agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt cagaaaaatc
     1081 aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt cagcaccaaa
     1141 gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa caaactcatt
     1201 aatcaaacca ttgacagttg ctgcatagcg actttctttt gtaccatggt caatacttga
     1261 aatagttgag aaagttccca agatgttatt tgggttttca gttttcaagt agaatggacg
     1321 gtatgttgcc acaactaagg cttggccaaa gatagctccc aagacttgcg cgataatata
     1381 aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag tgaaagcagg
     1441 gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa cccccatacc
     1501 ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt taagttcaac
     1561 gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa attcagtggc
     1621 atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt tttagatatt
     1681 ccttatttta tcaattttta ccctatttag caactgattt ctggaaatat ttttaaaaaa
     1741 ataggattca tagtttccca gttcaacgga cttgtaagcg tttttattct tcacgaaaga
     1801 cctcgttctc aaaaccagga aaaaagccca catctgtgag cttttcctgg ttcctagatt
     1861 ctaaaatcta ttatttttca tcttcttttt tcttgaagaa tcctaatcct agactagcta
     1921 acaaggtcat gataccagca ctagccaagg tagcattagc ttctgtacct gtgtttggca
     1981 attcctctct cttacctgtc tcataagtcg gaacttctgg gtctggattc actggagttt
     2041 cagtttttgg agtacctggg tctggagttg gtttatctgg tgttgataaa cggtcatacc
     2101 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     2161 tatctggatc tttgtacttg tcaatcttac cagatacaac ctcgtcccag tttcctgttg
     2221 tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta cggcttaagg
     2281 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     2341 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     2401 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     2461 agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     2521 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta tcgctaaatc
     2581 agcttttata acagactctg ccaacttctc ttggctacct tctttatagt aattggctgt
     2641 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     2701 gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     2761 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattgt agctggtacc
     2821 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     2881 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     2941 tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     3001 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     3061 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     3121 acggtgactt tgagtgttta ctactactct ttcattattc tctgaaaata cttgtacggt
     3181 ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc cgccttttcc
     3241 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     3301 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     3361 ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg tatttgtatt
     3421 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     3481 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     3541 ttgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     3601 gtcttcagta gtttatatgc tctgtcatca tctttagctt tagaagcagc tgagtttagc
     3661 caagttgggg tattaggagt atctttatca gcctgtttca agacattatt caagtcattt
     3721 gctagcctat tggttgtcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     3781 gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     3841 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     3901 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     3961 tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     4021 tcaaaccttt cttatacaag tcttcattct aaacgatttc ggctacacgg tgaatggttt
     4081 gattgtatac cgtcttctct taaccagcag tatccttagt tttaatgact actcgatagg
     4141 ttcctttaga accgttatag tcatctagtt ttaaaaaagc attccaaaca ttcaaaaata
     4201 gttggaactt cccttaataa atccgtcact ttctttttca agatagccca agcttgctca
     4261 ataggattca aatccggtga ataaggtggt agaggtaaga aaaagtgttt atcttggatg
     4321 cagagttcat ccaaaatgtt cttgggatga aaactagcat tgcccatgac aataacatga
     4381 ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa atcgcttgtc
     4441 atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc tgcaacaatt
     4501 gaagttcgct caaaacgacg tccgctaatc tttttataga ctttctcccc tctaggagcc
     4561 cgtgcataag gacgatagag gtagcggtcg attcctgttt catcaatata tacgattggt
     4621 aaatctttta ggttatccaa aatatcaaga aactcagcga ctttctctgg ctcttgttcc
     4681 ttaaagctcg tcatcttttt ttaaagtgac cttaatatac tttaaagctg cccatacgga
     4741 gggcagagca caatcaaaat gtgccgcaat ttcccgtaaa aaagcatctg gatgagcctc
     4801 tacaaaggct ttcaattcct ctaaaggaat ctttcggttt ttgacgactc gctttttcct
     4861 ctctatgtgt ccttgttcac atctttcttt tcccacgtga agagagttct gacgccaaca
     4921 ccaaaaaact tggctgcctc gacatggctg tgcccctctt tgatgtaatc taatgctcgc
     4981 tgtttaaaat cagtactata tgccatagct ttattataat atgcttgttt aaaactaagc
     5041 aactatatca atgttttgtt attagaagac ttactttttt tactgaaatc gaatttttcc
     5101 taacctcaac atcatataac cccaaaattc aatctgacct ggtttgaaga gatgttcgaa
     5161 gcagataagg ttttactaaa actaaaaaaa atgatataat caaactgatt aaatgtttta
     5221 aaaataaagg agaatttgaa tgtcttacca agaaaattac cagaaatggg ttgattttgt
     5281 ggagcttcct gactaccttc gtcaagattt ggaaaatatg gacgaaaaaa ctaaggaaga
     5341 tgccttctat acaaatcttg aatttggtac tgcaggtatg cgtggcttgg ttggtgctgg
     5401 tacaaaccgt atcaacatct acgttgttcg acaagctact gaaggattgg ctcgtttgat
     5461 tgagtcaaaa ggtggaaacg agaaagaacg cggtgtagca attgcctacg atagccgtca
     5521 cttctcacct gagtttgcct ttgaatctgc ggcagttctt gctaaacacg gcatcaaatc
     5581 ttacgtattt gaaagccttc gtccgactcc agaactatca tttgcagttc gtcatctcaa
     5641 ctgtttcgca ggtatcatgg tcacagccag ccacaaccct gctccattta acggttacaa
     5701 ggtttacggt gaagacggtg gacaaatgcc tccacacgat gcggacgctt tgactactta
     5761 tatccgtgca atcgaaaacc catttgcagt tgaagttgct gatgtggaaa ctgaaaaagc
     5821 ttctggcttg attgaagtta tcggcgaagc tgttgacgta gaatacctta aagaggttaa
     5881 ggacgtaaac atcaacccag ccttgattga agaatttggt aaagacatga agattgtcta
     5941 cacaccactt catggtactg gtgaaatgtt ggctcgtcgt gctcttgccc aagcaggatt
     6001 tgactctgtt caagttgttg aagcgcaagc aactgctgac ccagacttct caactgtaac
     6061 atctccaaac ccagaaagcc aagcagcctt tgcccttgct gaagaacttg gtcgtcaagt
     6121 tggtgcagat gttcttgtcg caactgaccc agacgctgac cgtgttggtg ttgaagttct
     6181 tcaaaaagat ggtagctacc tcaacctttc aggtaaccaa atcggtgcta tcatggctaa
     6241 atacatcttg gaagctcaca aaaacgctgg aactcttcct gaaaatgccg ctctctgcaa
     6301 atctatcgtt tcaactgact tggtaacgaa gattgctgaa agctacggcg caactatgtt
     6361 caacgtcttg acaggtttca aatttatcgc tgaaaaaatc caagaattcg aagaaaaaca
     6421 caaccacact tacatgatgg gatttgaaga aagcttcggt tacttgatta aaccatttgt
     6481 acgtgataaa gatgccatcc aagccgttct tgtcgttgct gaacttgctg cctactaccg
     6541 ttctcgtggt ttgacacttg ctgacggtat cgaagaaatc tataaagaat atggctacta
     6601 cgcagaaaaa acaatctctg ttactctttc aggtgtcgat ggtgctgaac aaatcaaagc
     6661 gattatggct aaattccgca acaatgctcc aacagaatgg aacgcaacag ctatcactgt
     6721 cgtagaagac ttcaaggcac aaactgctac tgttgctgac ggtactgtta caaacttgac
     6781 aactcctcca agtgatgtgt tgaaatacac acttgctgac ggttcatgga ttgccgttcg
     6841 cccttcaggt acagaaccaa aaatcaagtt ctacattgca gttgtagggg aaaccaacga
     6901 agaatcacaa gctaagatta ctaacatcga agcagaaatc aatgcatttg taaaataaag
     6961 acctataaaa gatgagacca accaacctca tcttttttcg tatcaaatct aagcaatttt
     7021 agaaacttta tggcatacta gacttatcaa tgaaagcgag gtaatctcat ggaacaattt
     7081 ttagataaca tcaaagacct tgaagtcact acagttgtgc gtgcgcaaga agctcttgat
     7141 aaaaaagaaa ctgcaacctt ctttatcggt cgcaaaactt gcccttactg ccgtaaattt
     7201 gcaggtacat tgtcaggtgt cgtagctgaa accaaagctc acatttactt catcaatagt
     7261 gaagaaccaa gccaactcaa tgatttgcaa gcattccgct cacgttatgg aatcccaact
     7321 gtaccaggtt ttattcatat tacagatgga caaatcaacg tccgttgcga ctcttcaatg
     7381 tcagcacaag aaatcaaaga tttcgcagga ttgtaaaaaa tcagctaaca agtcttaaaa
     7441 tcacaaaaac gcataatgta aggtgcaaaa accttgatat tatgcgtttt attgttccta
     7501 aaacagttac atcagcattt cattacagaa cattttaatt ttggaagtca aatcaatttt
     7561 taacaatgtt ttagaaatca cagtgggcta ttccatattc aattatccag attcaatcgc
     7621 tattatcgtt ttcctagatt acaaaaaaaa ctacgttttg ctaatcaaaa cgtagtctca
     7681 gccactattt tatcttactg tccttctttt acttctttgg ttgctacatc ttctccaaac
     7741 catttttggc tgatttcttg gaacttgccg tccttgtaaa gactagaaaa agcttcattt
     7801 atcttcttaa ccaaggttgt atcttcctta cgggctccaa ccgcaaaagc ttctgtttct
     7861 agtccaactg taaagacatt ataatcgttt aaaacacctt ctgcttctaa ataatagttt
     7921 gcatagacac ggtcaatcaa tagaccatca attcgatcgt ttttcaaatc aatcaaggct
     7981 tcattaaagg tttggtattg attcgcttcc ttattagcga caatattctt caaaatttct
     8041 ggatttgctt caaagtccgc ataaccagat gaaccagctt gagctcctaa tgtctttcca
     8101 gtcatatcct ttgcagtcgt gataccagat gattttttcg taaccaatac ctgctcattc
     8161 ttcatatatg agttactgaa agccaccttt tcacggcgtt cgtctgtagc ggaatagcca
     8221 ttccaaatca gatcaatcgt tccttttgtc aattcagctt ctttcaaatc ccaatcaatc
     8281 ggttgccaat ttaccgtgat tccgtatttt tcaaaaacag ctgtagctaa atcaatatca
     8341 aatcctgcat aagaaccatc tttctgagca aatcccattg gaacaaaagt actatcaaat
     8401 ccaatagtaa tagacttgtt agactggtac tttgaccaat tatctccact agtttcgcta
     8461 gaatttttcc cacaagctac taagaacaaa gcagtcatca gactgactaa tacaagcatc
     8521 cattttttca tccatttttc ctcctattta ggttctactt tcaataatac atctgcgata
     8581 ttttcagcaa actgcaaatc atgggtaacc acaatctggg tcatcccaag ttccctattt
     8641 tgcaagatta gcttctccac ttccaaacgt aattctggat ccagggcaga agttggttca
     8701 tcgtagccaa tgatttctgg gtcaatcatc atagcacgcg ccaaagccac ccgctgcttt
     8761 tgcccaccag atagtgagaa aggataggcc tctgcgtgtc ctcctagtcc taactgttcc
     8821 aagagtccac tcgccttctt ctcagcctct tcctgcttca ttcccatggt cttcacaggc
     8881 gataaagtca aattttccag aactgataga tgaggaaata gttgaaaatc ttggaagaca
     8941 aatcctagta ggttgcgctt ctgcaattca tccagctcta aaggttgtcc attataaaag
     9001 atttgccctg aatcaatggt ttcaagaccc gcaagcatac gtaaaagagt cgtcttacct
     9061 ccaccagaag gtccaacgat agccaggatt tgcttttcag gaatacttag actgaaatta
     9121 gacaggattt gtttgtctcc aaagacttta ttgatatttc gtaattctaa catggcagcc
     9181 tcctatctat aataactata cttcttctca acttttttgg aaataattgt cacaatccca
     9241 atcaaaatca aataaatggc tcctgccaag aacataggaa ctagactagc atcgcggtta
     9301 gcagctgttc gactagccaa gataaggtct gaaattccga gagcatagac caaagaagta
     9361 tccttgacca aactcataac ttcattaaag acactaggaa gaacgatctt ggtcacttgg
     9421 ggcaagataa tatagcgcac tctgtcaaaa gggctaaact tcaagacctt ggcggcctca
     9481 tactgtcctc ttggaatagt gtcaattccc ccacggaaaa tttctgcaaa gtaagctgca
     9541 tagttgagaa caaaggcaat aatagctgca ggaaggcggt ctaaacgaat cccaatactt
     9601 gggagcacat aatagataaa aattagttgc aagagtaagg gggttcctcg cataacccag
     9661 atgtaaatgt taatcagata atggaggggc ttccaatgga cttgcaaggc aaaagcaatc
     9721 aaaacgccca agggaatcga aaatagcaag accagtgcaa agacctgtac agtcgtgctc
     9781 gcaccattca gtaaactcgg taatatctca aataaataag acatactgca cctcctaaaa
     9841 gtaattgttc ctattatagc atgaataaac aagattacca agtatttttg taaaaagatt
     9901 ctaccatata caaataaaaa ccgtagctat caaaagagat aaactacagt tctatatgtt
     9961 taaacattct tacaatctct caaacagttc ttgcatttgc acatcatctg gaaccagttt
    10021 taagtaagtg tgagcatgga cttttgcttc ttcaaaatgt cccaattcac gcaagagata
    10081 gatatagtgt tccagaaatt ctggattgtc cttcaaatct cctgtcaact cttgataatg
    10141 ctcataagca gtatccaaat cgtccatttc ttgataagaa cgagcaatca tccacttggt
    10201 caaaagattt tctggctcct cactctgcaa gtctagaata tcctcataac gctcctgctc
    10261 cagataaata gtggctaaac gaagcaagat ttcttctgta tcctcagcgt cttcttttgc
    10321 agcaagaaga taattttctg caccactagc atcatgcaat tcataagaaa attgtgaagc
    10381 agctagcaag aggcgagttt caaagggatt tttctctaat ccttgcttag cgatacgcag
    10441 ggcttcttga acttgatgtt ccttatgtaa agcctgactg tacccatact catagccttc
    10501 aaagtcagga gaaatggtat caagctgctt aaagtagagg gtggcttttt gatattcttc
    10561 ttgatcaaaa taaagactgg ccaactcaaa agctgttaag tcatcgtatt ctaactccag
    10621 ggctttttct aaaaactcag tagccgtttc aaatttccct aactgagcat aggcaaagcc
    10681 aattcgttga taggtggaaa tgcccgtttg ctcataaatc gagcgattat ctaactgggc
    10741 ataggcttga atagccgctt ggtaattttc caactcacta tccaactctg ccaaacccaa
    10801 catcaagaga gaatcctctg agtaggtcaa ggcctccaat aatttctcac gtgccacatc
    10861 tgtcaaacct tccagctggt aaaggtctgc cttcaaaacc aaagacgaga cataccagtc
    10921 actgtcagct tggatttcct caagataggt aaaggcttct tctatttgac catcctcgct
    10981 agcaattgca gctagattaa gatgaacctc tggaaaatcc tctacaattt tcaggtaaat
    11041 ttccttggcc tgaggataga aaccaatccc ttcaagataa gttgccaact catacagaag
    11101 atcacttgaa tcattttcta aagctttggc gaaataatac tcagccttag ttaaatcttg
    11161 ctcctccaaa gcctgtaaca tctgttgact attgttcact cttgacttcc tcaccctcta
    11221 attcatatag accactgact accttatacc attcgaaaat ggctgaaatg acaaccttag
    11281 cagaggcata aaccggaata ccaagtaaaa ctccccagat accaaacata gatcctgaag
    11341 ttaacaaaac aaagagaaca ttaatagggt ggatgtttaa ttgacttccc aaaatcaatg
    11401 gagagacaaa acggccttca atagtttgtt ctacgataaa gacaatcact actttcaaaa
    11461 gcatgactgg accagcaatc aaacccaata ctagagcagg aagcatggct agaaagctac
    11521 caagataagg gaccagattt aaaataccag cagtaacccc cagcgtaacc gcatagcgta
    11581 gaccaataat cttgaagaag atgataaata ttactgctac aataatagcc actgtcactt
    11641 gccctcgaac atagttggac aactgttgat tcacatctga taaaacttgt ccaacaggtt
    11701 ccttcaattt tcttggaatg aattgggtca aatagttacg caagcctttc ccatcacgca
    11761 agagataaaa gagcatgaaa ggaacgataa tcaaggcaac aatcacttga gaagctccac
    11821 taataaaggc actcacccag ttgactgcct gagatgaaac cttacttgcc aaaactgtag
    11881 cctggctaga aaaattggtc aaaacttgct ctaattgagg tctgaaatca tctggcaggt
    11941 gctgggctac caatccatta acaatcctat ctatatcttc taagtaaaca ggaacgtttc
    12001 ttgcaaaggt caaaacctga cgttgcagat ttggaatggc gactgccaag ccccaaatga
    12061 taaagagagc gatgataaca aagacaatag tgatagctat aacacgatta accttatgct
    12121 tctccatcca atcaacaata ggattcaaca aataatataa caaaccagac aaaatgactg
    12181 gcaacatcac aactgctaaa aaatctaaaa caggtgaaaa tagaaaacta atcttactta
    12241 aaataaaaag attcaatccc aataataagg ttactaaaaa taccgtaatt gccttgttat
    12301 ctaaaaacca cttgaaaaac caagataggc taaaatgttt ctctttttgc tccataaata
    12361 cttcctttct attgttctct cattatacca tttttgaaac aaaataactc ttttttaaag
    12421 tgcttacatc taattgaatg tctttaattt gcttgttttt gtgatataat agtcttatct
    12481 ttacacagag gtatagtaat gaaagaaact gtttattttg gaacttatac acgtcgtact
    12541 tctcaaggga tttacaaggc agactttgat acagaaactg gtcagctttc aaatctagaa
    12601 ctttttgcag ctgagccaag tccaacctac cttgcctttg accagcacca acatttatac
    12661 actgttggta gccaagacga taagggggga attgcagcct atcaaactga cgggactgtg
    12721 ttaaatcatg ttgttgaaga aggagctccc cactgttatg ttgctgtcga tgaaaagcgt
    12781 gatttggttt acgcagccaa ctatcacaag ggacaagtcc ttgtttataa acgccaggaa
    12841 gatggtagtc ttctacttag tgatatggat caacacagtg gccaaggtcc acatgaaaat
    12901 caagcttccc cccatgttca ctatacagat ttaacacctg accactatct agtgacctgc
    12961 gacttgggaa ctgaccaagt catcacctat gacctcgatc aagaaggaaa attatctaag
    13021 ctctatacct atcacagcaa gccaggagca ggctcacgcc atatcatttt ccataaccac
    13081 tataaaatcg cttatctcat ttgtgaactc aatagtacta tcgaagtttt aatctacgat
    13141 ggcgttggcg aatttgtacg tatgcaggtt atttcaactt taccagaagc ttacgaaggc
    13201 tttaatggaa ccgctgctat tcatctctct aaagacggta aatacctcta cgcttctaac
    13261 cgtggccatg attctatcgc agtatatacc atccttgcgg acggtagctt agagttatta
    13321 gaaatcgttc caacgcatgg tcagactcca cgtgattttg atttgacacc cgaccaaaaa
    13381 tttctcattg ttgtccatca agactctgac aatgcaactg tctttaaacg taattgtgac
    13441 aatggtcgtc tagcagaact ctccaacgaa ttccatgttc ccgaagcagt ctgcatccgt
    13501 tttgctcctt aaaacaccat aaaaaatggc tctataatat ttgtagtggg taaatcccct
    13561 atggatatta tggagcctat ttttgtgtag aaaaaaagtc ccataagatc tataatgaaa
    13621 agcgatcaaa caactcatta gaaagattca tatggaacaa ttacatttta tcacaaaact
    13681 actcgatatc aaagacacaa atatccaaat tatagatgtc gtcaataggg attcacacaa
    13741 agaaatcatc gccaaactgg actacgacgc accatcttgc cctgagtgcg gaaaccaatt
    13801 gaagaaatat gactttcaaa aaccttctaa aattccttat cttgaaacga ctggtatgcc
    13861 tactagaatt ctccttagaa aacgccgttt caagtgctat cactgttcaa aaatgatggt
    13921 cgctgaaact tctatcgtca agaagaatca ccaaatccct cgtatcatca accaaaaaat
    13981 tgcgcaaaag ttgattgaga agatttctat gaccgatatt gctcatcagc tggccatttc
    14041 aacttcaact gttattcgta agctcaatga ttttcacttt aaacatgatt tttcttgtct
    14101 tcctgagatt atgtcttggg atgagtatgc ttttacaaag ggaaagatga gcttcattgc
    14161 gcaagatttt aacaatctca atatcatcac tgttcttgag ggtagaacac aagctatcat
    14221 tcgaaatcac tttcttcact acgatagagc
//
LOCUS       DAVKFH010000041        14129 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKFH010000041 DAVKFH010000000
VERSION     DAVKFH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.041"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            369..638
                     /gene="blpJ"
                     /locus_tag="VW006_001449"
     CDS             369..638
                     /gene="blpJ"
                     /locus_tag="VW006_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9380280.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            707..955
                     /gene="blpK"
                     /locus_tag="VW006_001450"
     CDS             707..955
                     /gene="blpK"
                     /locus_tag="VW006_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEU9380281.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            1199..1348
                     /gene="blpO"
                     /locus_tag="VW006_001451"
     CDS             1199..1348
                     /gene="blpO"
                     /locus_tag="VW006_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9380282.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1452..1571
                     /locus_tag="VW006_001452"
     CDS             1452..1571
                     /locus_tag="VW006_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9380283.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2039..2410
                     /locus_tag="VW006_001453"
     CDS             2039..2410
                     /locus_tag="VW006_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380284.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            3039..3422
                     /locus_tag="VW006_001454"
     CDS             3039..3422
                     /locus_tag="VW006_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9380285.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            3474..4163
                     /locus_tag="VW006_001455"
     CDS             3474..4163
                     /locus_tag="VW006_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9380286.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            4205..4438
                     /locus_tag="VW006_001456"
     CDS             4205..4438
                     /locus_tag="VW006_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380287.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            4589..5200
                     /locus_tag="VW006_001457"
     CDS             4589..5200
                     /locus_tag="VW006_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9380288.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            5361..6155
                     /locus_tag="VW006_001458"
     CDS             5361..6155
                     /locus_tag="VW006_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9380289.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            6152..6787
                     /gene="trmB"
                     /locus_tag="VW006_001459"
     CDS             6152..6787
                     /gene="trmB"
                     /locus_tag="VW006_001459"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9380290.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6913..7392
                     /gene="rimP"
                     /locus_tag="VW006_001460"
     CDS             6913..7392
                     /gene="rimP"
                     /locus_tag="VW006_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9380291.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            7436..8572
                     /gene="nusA"
                     /locus_tag="VW006_001461"
     CDS             7436..8572
                     /gene="nusA"
                     /locus_tag="VW006_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9380292.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            8594..8887
                     /locus_tag="VW006_001462"
     CDS             8594..8887
                     /locus_tag="VW006_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9380293.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            8880..9179
                     /locus_tag="VW006_001463"
     CDS             8880..9179
                     /locus_tag="VW006_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9380294.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            9196..11988
                     /gene="infB"
                     /locus_tag="VW006_001464"
     CDS             9196..11988
                     /gene="infB"
                     /locus_tag="VW006_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9380295.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            12239..12589
                     /gene="rbfA"
                     /locus_tag="VW006_001465"
     CDS             12239..12589
                     /gene="rbfA"
                     /locus_tag="VW006_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9380296.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            12868..13647
                     /locus_tag="VW006_001466"
     CDS             12868..13647
                     /locus_tag="VW006_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380297.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            13649..13831
                     /locus_tag="VW006_001467"
     CDS             13649..13831
                     /locus_tag="VW006_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380298.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            13791..13979
                     /locus_tag="VW006_001468"
     CDS             13791..13979
                     /locus_tag="VW006_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380299.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
BASE COUNT         4340 a         2370 c         3131 g         4288 t
ORIGIN      
        1 agatagttag caaaatttta attatattat caatcgttta tgtaatcgtt gatgctttac
       61 ttagttgatg gattggtagg aataaaaaac ttaactataa actattattt actaatattt
      121 tttgctttct gcaagttaat aaatagtata gtaaaacttt ttattgcaag gagaataacg
      181 aaattaagat attttagata ctcaagatta ttgaaatctt agactaaaaa ataggtactg
      241 ctacctttaa gaagatagta gacgtatata cttttttaag aaaatcaaaa agatactaaa
      301 aaaaatctat ttgtttattg aatttaagac tttggtaaca aattgaaaat aaaaaggagg
      361 tattcatcat gaatacaaaa atgttgtcac agttagaggt tatggatact gaaatgcttg
      421 cgaaagttga aggggggtat agctccactg attgtcaaaa tgcactgatt acaggagtca
      481 ctacgggaat tataactggt ggaactggag caggtttggc cactttagga gtagctggac
      541 ttgctggtgc atttgttgga gcacacattg gtgcaattgg gggtggatta acatgcttag
      601 gaggtatggt tggtgataaa ttgggactaa gttggtaaga cttatagtaa aattatgagg
      661 attctattct gatttatttt agttttttaa aaaaggagta tttgtcatgg atacaaaaat
      721 gatgtcacaa ttttctgtta tggatactga aatgcttgct tgcgttgaag gtggcggatg
      781 caattgggga gattttgcca aagcaggtgt tggaggagca gctgtagttg ctgctttagg
      841 ttgtgcagcg ggtggagtga aatatgggaa aattctagga ccatggggcg ctgcaatagg
      901 aggaattgga ggagcagtgg tttgtggata tttagcctat accgctacat cataaaaatg
      961 aaagctaaat tttatgaagg ctatattctt tattattctc ttcgcttttc aaacctatct
     1021 tatttatcta tccataagta tctctgataa aaaacaaaag actattgaat taacatcatt
     1081 gaattgtttt gtcatattat ttctaatcta tgataaatta atttttctct ttattgccta
     1141 tgtttttttg ataattttta tattaaactt gttccgtaac tgaaaagtga catactatat
     1201 gaatacaaaa atgatgtcac aattttctgt tatggataat gaaatgcttg cttgcgttga
     1261 aggtggagat attgattggg gaagaaaaat tagttgtgca gcaggggttg catatggcgc
     1321 aattgatggg tgtgcaacaa cggtttgaca tttctattgg gaccatttgc tataggaata
     1381 ggtgtaactg gtgctgcagg tggagctata cttggaggtg tagcctatgc agcaacatgt
     1441 tggtggtaat tatggatttt aaaagtttta ttattggttt agtagttggt atatttggtc
     1501 cttatatgga tgacttaatt agaaaaatat tttcaaaatc ttccaaaaag gatacggata
     1561 gtactctcta aaaaataaat ttctcccctg tatttgctag cttaatttac aggggagttt
     1621 ctttaatatt gtttcgcctg tcaaaatgat atgtgatagc aggatgaaaa gtctacaaac
     1681 aagttggtat caatacaata taaagaggaa agtatgtttt tcatgcttgc ttttttaatt
     1741 tttacaattc aagaggtttt gatgaccatt tatgatttga gtgatccaag gtcaaaataa
     1801 gtgctatact agcagtgtaa atgttcttgc tcaactaaga taaatcgcac atttaggagg
     1861 aaaacttatg aagaaaaaaa tactgattat tttcgttctg tatctgatca tgtccatctt
     1921 tctttatccg cttagggaga gtatttggta taatctattt tataccatag cctatatgat
     1981 tgcggttatg atctattttt ctttaattaa aaagaaagaa aagaaatgaa agattttctt
     2041 gattaaaaag aatcatattc tttgtgagat tgttcctggg tcagttgcct ttgcttgttt
     2101 ctacttatct atttctattt cgtcagtttt ccaatttctt ttagtagtta ttaacttgac
     2161 ttctattttg gttactgttt atcttactag ggaaatgaga ttaagagagt ttgaagatga
     2221 tgatttggtt agtcctagaa ccaatcaact catgtttatc ggcttgacag gctttatgtc
     2281 tattatttgt ttgtatagag gtatcacagc aggagaatct tatcaacaac taatcgctta
     2341 tattggtgct attctttgct tgatcatcat gcttctactc atttggggtt tgaagtatta
     2401 taaaaagtag gggctaatga agtttttgca atttaggagg tttcgatgac aattcaagat
     2461 ttggatgaaa aattttaaaa aacaatgata tactaaactt gtcaaagttg caacgggtgc
     2521 tgctacatta tgttaaaaat cgctcttgat tgataagttg gatttgttag gaaaggagta
     2581 aataattatg gataagaaaa agatagtttc gactataata tgtatagtat ttcttgtagt
     2641 gtcagtagat aattttttta gagacttaac cccgttactg ttcattttaa atattattgg
     2701 cctgtcttgc ttttcagtgc taacatacat taacataaaa gagatactct taaatataag
     2761 taaatgatga tttggttagt cctagaacca atcaacttat gtttatcggc ttggcagatt
     2821 ttatgtttgt catttgtttg tatagaggta tttcagaaac agaattctat caacaactaa
     2881 tcgcttatat aggtgtattc tctgcttgtt tatcacgctt ctgctcatgt ggggcttgaa
     2941 gtattataaa aagtagcttt ctatcctttt ctgacaggat atttgagtag aaggctctat
     3001 taaattgtta gtgaaataaa tgaatggagg tatttaatat gaaatacaga ttattttttg
     3061 ttattttctt gagtagtgtg ttggatattc ttttagggac atttttacaa atctctatcg
     3121 tatctattgg gtggcttgtt ctttacagtg gtttgtttga agcaggagtt ttccttcttg
     3181 ctaataaagg ggtggcggta aaaatcaagg aagtagatat tcgaaatcgc tttaaattta
     3241 tttttggaaa aaccttatgg tttcaaattc ttttgctcat ctttttgata atcaaacttt
     3301 atcttggttt ggatgcgagg ttgattttat tctatggaca tattttcatt gtctttaatg
     3361 ccttaatgta tctattatct agtagtcagg ttagccttaa aaaaaacaaa ctgtcttctt
     3421 aatcttacag cagtagagcc tgacaaggtg aggtcatagt agggaggatt tctatgaaaa
     3481 agtatcaact tctattcaaa ataagtgcag ttttctctta cttatttttc gtatttggtc
     3541 tttctcagat gacgcttatt attcaaaatt attggcaatt ttcttcccag attggcaatt
     3601 tcgtctggat tcaaaatttc ttgagtttgc tatttagcgg agtcatgatt tggattctgg
     3661 ttaagacagg tcatggttat ctctttcaca ttccaagaaa aaaatggctt tggtattcga
     3721 ttttgacagt attagtggta gtgctccaga tctcttttaa cgttcagaca gctaaacatg
     3781 ttcagtcaac tgctgaaggt tgggctgtat tgatcggtta tagtgggacc aactttgctg
     3841 agctaggtat ctatataact ttgttctttc tgactccact tatggaagag ctaatctata
     3901 gaggattact gcaacacgcc ttttttaagc attcgagatt tggccttgat ttgcttcttc
     3961 cgtcaatttt atttgctctt cctcattttt caagcctgcc tagtttgtta gatatcttca
     4021 tctttgcaac atctggcatc atctttgcta gtttgacccg ctataccaag agcatttatc
     4081 cttcctatgc ggtgcatgtg atcaataata ttttcgcaac attaccattt ttgctgactt
     4141 ttttacatag ggtcttgggg taaaaaaaca aaaggcttgc ttttcagcca tagaggagat
     4201 tatcatgtat aaacacttat ttttcctaga ttccaaaact ttagatcggt tgacacccta
     4261 tattctagtc ttggcttctg acaccattgc ttttaatgtt tttgtgctaa cctttgtatc
     4321 tgcggtggtt tttaatttcc taaattccat gctagcttta atggctatat tcataggggc
     4381 tggctatgtg gtcggatttt ggttactaat actcaatgaa aatcaaagag caaactagga
     4441 aactagccgc aggctgtact tgagtacggc aaggcgacgt tgacgtggtt tgaatttgat
     4501 tttcgaagag tataaaatgg tttgttttgg aaaggctaga actaaagaat gacgcgtagg
     4561 aagtctgttt ggtgaggagg atagttttat ggagtttttt gataaatttc atgccttgtg
     4621 ttttggattt ttagtactaa taattgtcat tacagttcct tatacgatta accatggggg
     4681 tttttttcaa aatgaatctg cattgattct tgtaagtctt cttgtaacct cgctgagtgt
     4741 tgcttatgct agaaagtttg aaatgatttc ttttgggatg ttaagcaaga aacaactttt
     4801 gcttttcatt gcaatctttc ttctaagtgt acttgagacg ctggtttata ttcatttctt
     4861 cgctgtttct tctggctcag gggtccaaca cttggcggaa gtcagcagag gaatttccct
     4921 gtctttgatt ttgactacct cagtttttgg ccccatccag gaggaactca ttttcagagg
     4981 acttcttcaa ggtgcggttt ttgacaattc ttggttaggg cttgtgctaa cttcctctct
     5041 cttttctttc atgcatggac cttctaatgt cccttcgttt attttttatc tacttggggg
     5101 tttgttgctg ggctttgctt ataaaaagag tcaaaaccta tgggtttcta ctctagtcca
     5161 catgttttac aacagttggc cactcctata ttatttataa aaattatgaa aaggtaagca
     5221 gaagatggtg cttacctttt tcaagctata atgataccca agccaatcca gaggcttttc
     5281 tttgagaatc gggttagagt ggttgacgaa taggccaaaa actagtagaa tagtaaggaa
     5341 actttatacg gaggaaagaa atggatttgg gtgataatga gctaacactg actcccatac
     5401 ctgggaaaag tggcaaggct tatatgggta gctatcctga cgggaagcgc atctttgtaa
     5461 aaatgaacac ctctccaatc ctacctggtc tagctagaga acaaattgct ccacaattat
     5521 tatggagtcg tcgtttggca gatgggcgtg atatgtgtgc tcaagaatgg ttgacaggca
     5581 agatattgac cccctatgat atgaatcgta agcaaatcgt caatatttta acccgtcttc
     5641 atcgctcacg tccgttgatg acacaattga gtcgtttggg ctatgccatg gaaacacctg
     5701 tagatttact acagtcttgg caggaaacgg ctccagatgc tttgcgtaaa aatcatttta
     5761 tcagtgaagt gatggctgat ttacgtcaga ctattccagg atttagagag gaccatgcga
     5821 ccattgtcca tggagatgta cgacatagta attggattga gacagatagt ggcttgattt
     5881 atttagtaga ttgggattcg gttcgcttga ccgatcgcat gtttgatgtg gcccatatgc
     5941 tctgccatta tatttcagaa catcagtgga aggaatggtt gacctactac ggttacaagt
     6001 acaatcaaac ggtattaagt aaattgtatt ggtatggtca attgtcttat ttgagtcaga
     6061 tttccaagta ttatatgaac caagatttag aaaatgtcaa tcgggagatt catggtttgc
     6121 gtcatttccg agacaagtat ggaaagagaa gatgagagtt agaaatcgta aaggggcaac
     6181 agaattacta gaggcaaatc cccagtatgt ggtcctcaat cccttggaag ccaaggcaaa
     6241 atggcgggac ttgtttggca atgataatcc cattcatgtg gaagttggaa gtggaaaggg
     6301 tgcctttgtt tcaggtatgg ccaagcaaaa ccctgacatc aactatatcg ggattgatat
     6361 tcaaaagtct gttttgagct acgctttgga caaggtgctt gaagttggag tgcctaacat
     6421 caagctcttg tgggtagatg gttctgactt aactgactat tttgaagacg gtgagattga
     6481 tcgcttgtat ctgaactttt cagatccatg gccgaaaaaa cgccatgaaa agcgtcgttt
     6541 gacctacaag accttcttgg ataccttcaa acgtatcttg cctgaaaatg gagaaattca
     6601 tttcaagacg gataaccgtg gcttgtttga gtacagttta gtgagctttt ctcaatatgg
     6661 catgaaactc aatggtgtct ggttagattt gcatgccagt gattttgaag gcaatgtcat
     6721 gacagaatac gagcaaaaat tctcaaacaa ggggcaagtt atctaccgag ttgaggcaga
     6781 attttaagag atagcctaaa attaggctgt acaagtgctt ttgctttaca taagttggca
     6841 aacgtgctat actgatagta agaatatgaa aagtgaggcg gggaaatatc ttcgcctctt
     6901 gcttatgagg aggtggacgc aatcgcaaca atcgtagaat tagtcagaga agttgtagaa
     6961 cctgtcatag aagctccttt tgaactcgtg gatatcgagt atggaaagat tggcagtgac
     7021 atgattctca gtatttttgt agataaaccc gaaggaatta ccttgaacga cacggcagac
     7081 ttgacagaaa ttatcagtcc tgtcctagac accatcaagc cagatccctt cccagaacaa
     7141 tatttcctag aaattaccag tccaggtttg gaacgccctt tgaaaaccaa ggatgccgtc
     7201 gctggagcgg ttggaaaata catccatgtc gggctctacc aagccatcga taagcaaaag
     7261 gtctttgaag gaaccttgtt ggccttcgaa gaggacgagt tgactatgga atatatggac
     7321 aagacgcgta agaaaaccgt ccaaattcca tacagtttag tatcaaaagc acgtttagca
     7381 gttaaattat agaaaaagaa aggatagctt ttgaggattc aaaagtgaag aaaacatgag
     7441 taaagaaatg ctagaggcct tccgcatttt ggaagaagac aagggaatca aaaaagaaga
     7501 tatcatcgac gcagtagtag agtcgcttcg ttccgcttat cgcagacgct atggtcagtc
     7561 agacagcgta gctattgact tcaacgaaaa aacaggtgac tttacagttt atactgtccg
     7621 tgaagttgtt gatgaagtat ttgatagccg tttggaaatc agcttgaaag atgctcttgc
     7681 cattaattca gcttatgaac ttggagacaa aatcaagttt gaagaagcac cagctgagtt
     7741 tggtcgtgta gcagcccagt ctgccaaaca aaccatcatg gaaaaaatgc gcaagcaaac
     7801 acgtgccatc acttacaata cttacaaaga acatgagcaa gaaatcatgt ctggtacagt
     7861 agaacgcttt gacaaccgct ttatctatgt caaccttggt agcatcgaag cccaattgtc
     7921 aaaacaagac caaattcctg gagaagtttt tgcttctcat gatcgtatcg aagtttatgt
     7981 ttacaaggtt gaagacaacc ctcgtggtgt gaacgtcttt gttagccgta gtcatccaga
     8041 aatgatcaaa cgtttaatgg agcaagaaat tccagaagtt tatgatggaa ctgttgaaat
     8101 catgagcgtg gctcgtgaag caggtgaccg tacgaaggtt gctgttcgta gccacaatcc
     8161 aaacgtggat gctatcggta caatcgttgg acgtggtggt gctaatatca agaagattac
     8221 tagcaaattc cacccagctc gttacgatgc taaaaatgac cgcatggtac caatcgaaga
     8281 aaatatcgat gttatcgagt gggtagcaga tccagctgaa tttatctaca atgccatcgc
     8341 tcctgctgag gttgaccaag ttatctttga tgaaaacgac agcaaacgtg ccttggtggt
     8401 tgttccagat aacaagcttt ctcttgccat tggtcgtcgt ggacaaaacg tgcgcttggc
     8461 ggctcacttg actggttacc gtatcgatat caagtctgct agcgaatttg aagccatgga
     8521 agacgctgct tcagtagagt tggaagtaga aaacgatact gtagaagaat aaaagctgct
     8581 agaggaggga aagatgaaaa caagaaaaat ccctttgcgc aagtctgttg tgtctaacga
     8641 agtgattgat aagcgtgatt tgctccgcat tgtcaagaac aaggaaggac aagtctttat
     8701 tgatcctacg ggcaaggcca atggccgcgg cgcttatatc aaactagaca atgcagaagc
     8761 cctagaggcg aaaaagaaga aggtctttaa ccgcagcttt agcatggaag tggaagaaag
     8821 cttttatgac gagttgatcg cttatgtgga tcacaaagtg aaaagaagag agttgggact
     8881 tgaataagca aaagataagt aatctcttgg ggcttgctca gcgagcaggg cgcatcatat
     8941 cgggtgaaga attggtggtc aaggccattc aagacggcaa ggccaagttg gtctttctag
     9001 ctcatgatgc tggacccaat ctgaccaaga agattcaaga taaaagtcat tattatcaag
     9061 tagaaattgt aaccgtgttt tcaacactgg aattaagcat agcagtcggg aaatcgagaa
     9121 aggttttggc tgtaacagat gctggattta caaagaaaat gaggtctctt atggaataga
     9181 agaggaggac atgatttgtc taagaaaaga ttgtacgaaa tcgcaaaaga acttggaaaa
     9241 gaaagtaaag aagttgtagc gcgtgcaaaa gagttgggct tggatgtgaa aagccactca
     9301 tcaagtgtgg aagaagctgt cgctgcaaaa attgctgcca gctttaagcc tgcagctgct
     9361 ccgaaagtag aagcaaaacc tgcagcacca aaagtaagtg cagaaaagaa agccgaaaaa
     9421 tctgagccag ctaaaccagc tgtagctaag gaagaggcaa aaccggctga gccagttgct
     9481 ccgaaaacag aaaaagtagc agtgaaaccg caaagtcgta atttcaaggc tgagcgtgaa
     9541 gcccgtgcta aagagcaggc agaacgacgc aagcaaaata agggcaataa ccgtgaccaa
     9601 caacaaaacg gaaaccgtca gaaaaacgac ggccgtaatg gtggaaaaca aggtcaaagc
     9661 aaccgcgaca atcgtcgctt taatgaccaa gctaagaagc agcaaggtca gcaaaaacgt
     9721 agaaatgagc gccgtcagca agaggataaa cgttcaaatc aagcggctcc acgtattgac
     9781 tttaaagccc gtgcagcagc cctaaaagca gagcaaaatg cagagtacgc tcgttcaagt
     9841 gaggaacgct tcaagcagta tcaggctgct aaagaagcct tggctcaagc taacaaacgc
     9901 aaggaaccag aggaaatctt tgaagaagcg gctaagttag ctgaacaagc acagcaagtt
     9961 caagcagtgg ttgaagtcgt ccctgagaaa aaagaacctg cagtggatac acgtcgtaaa
    10021 aaacaagctc gaccagacaa aaatcgtgac gattatgatc atgaagaaga tggtcctaga
    10081 aaacaacaaa agaatcgaag tagtcaaaat caagtgagaa atcaaaagaa tagtaactgg
    10141 aataacaaca aaaagaacaa aaaaggcaat aacaagaaca accgtaatca gactccaaaa
    10201 cctgttacgg agcgtaaatt ccatgaattg ccaacagaat ttgaatatac agatggtatg
    10261 accgttgcgg aaatcgcaaa acgtatcaaa cgtgaaccag ctgaaattgt taagaaactt
    10321 ttcatgatgg gtgtcatggc cacacaaaac caatccttgg atggggaaac aattgaactc
    10381 ctcatggtgg attacggtat cgaagccaaa caaaaggttg aagtggataa tgctgacatc
    10441 gaacgtttct ttgtcgaaga tggttatctc aatgaagatg aattggttga gcgtccacca
    10501 gttgttacta tcatgggaca cgttgaccac ggtaaaacaa cccttttgga tactcttcgt
    10561 aactcacgtg ttgcgacagg tgaagcaggt ggtattactc agcatatcgg tgcctaccaa
    10621 atcgtggaaa atggtaagaa gattaccttc cttgatacac caggacacgc ggcctttaca
    10681 tcaatgcgtg cgcgtggtgc ttctgttacc gatattacga tcttggtcgt agcggcagat
    10741 gacggggtta tgcctcagac tattgaagcc atcaaccact caaaagcagc taacgttcca
    10801 atcatcgtag ctattaacaa gattgataaa ccaggtgcta acccagaacg cgttatcggt
    10861 gaattggcag agcatggtgt gatgtcaacc gcttggggtg gagattctga atttgttgaa
    10921 atctcagcta aattcaacca aaatatcgaa gaattgttgg aaacagtcct tcttgtagct
    10981 gaaatccaag aactcaaggc agacccaaca gtgcgtgcaa tcggtacggt tatcgaagcg
    11041 cgcttggata aaggaaaagg tgcggtcgca acccttcttg tacaacaagg taccttgaat
    11101 gttcaagatc caatcgttgt cggaaatacc ttcggtcgtg tccgtgctat gaccaacgac
    11161 cttggtcgtc gtgttaaagt tgctggacca tcaacaccag tctctatcac aggtttgaac
    11221 gaagcaccga tggcgggtga ccactttgcc gtttacgagg atgaaaaatc tgcgcgtgca
    11281 gcaggtgaag agcgtgccaa acgtgccctc atgaaacaac gtcaagctac ccaacgtgtt
    11341 agccttgaaa acctctttga tacccttaaa gctggggaac tcaaatctgt taatgttatc
    11401 atcaaggctg atgtacaagg ttctgttgaa gccctttctg cctcacttca aaagattgac
    11461 gtggaaggtg tcaaagtgac tatcgtccac tcagcggtcg gtgctatcaa cgaatcagac
    11521 gtgacccttg ccgaagcttc aaatgccttt atcgttggtt tcaacgtacg ccctacacca
    11581 caagctcgtc aacaagcaga agctgacgat gtggaaatcc gtcttcacag cattatctac
    11641 aaggttatcg aagagatgga agaagctatg aaagggatgc ttgatccaga atttgaagaa
    11701 aaagttattg gtgaagcggt tatccgtgaa accttcaagg tgtctaaagt gggaactatc
    11761 ggtggattta tggttatcaa cggtaaggtt gcccgtgact ctaaagtccg tgttatccgt
    11821 gatggtgtcg ttatctatga tggcgaactc gcaagcttga aacactacaa agacgacgtg
    11881 aaagaagtga caaacggtcg tgaaggtgga ttgatgatcg acggctacaa tgatattaag
    11941 atggatgatg tgattgaggc gtatgtcatg gaagaaatca agagataaga ttttttgctc
    12001 ctttcttagg tggtgaggga cgcaagcaaa ccgatggttt cattgcttat ttttgagcct
    12061 agggtctcaa aaatcccctg tgatgggact gataaatcag ttccatcact ttcaccacgg
    12121 cgaaagaagc agatgacttc aaattgaact tcgtttcaat ttaaactgaa aatcaagaag
    12181 tttaaaatag ctaggtctgc tggcctagct tttggttcaa agtagagaaa ggaatatcat
    12241 ggcaaatcat ttccgtacag atcgtgtggg catggaaatc aagcgtgaag tcaatgagat
    12301 tttgcaaaag aaagtccgtg atccacgtgt ccaaggtgtg accatcacag atgttcagat
    12361 gctgggtgac ttgtctgttg ccaaggttta ttacaccatt ttgagtaacc ttgcttcgga
    12421 taaccaaaaa gcccaaatcg ggcttgaaaa agcaactggt accatcaaac gtgaacttgg
    12481 tcgcaatttg aaattgtaca aaatcccaga tttgaccttc gtcaaagacg agtccatcga
    12541 gtatggaaac aagattgacg agatgctacg caatctggat aagaactaaa gaagaggggt
    12601 tgcccctctt ttttggtgga ggaaaatagg ttgaatttga aatggaaaaa tattctttta
    12661 aaatatattg aaactagaat agtacgcctc tacttctaaa acattgttag aaatcgattt
    12721 gactgtcctg atcgatttgt cctgttcttg tttcatttta ctataaaaaa ggggttctgt
    12781 attttttaat gttatctaat tagaaaatgc tttttttgta ggaaatataa tatgataagg
    12841 tgtaaaaaag aaataaggag tttgtatatg gctgaacaag acttagctat gcaagtattg
    12901 caacaagtgg tgaaactacc tgttgttaag gttgatcgtt cgaaattttt agtggataag
    12961 ttttccaaag aattggatcc aaaagatatt cctaccttat tggaacaagg tacaacgact
    13021 cttctatctc aagaaatatt agatcgtgta gctaatgctt gtattcggga caatgtatta
    13081 ttagcgagtg ggacttctgt tttggcagga ttagctggag ggcttgctat ggcaattacc
    13141 attccagctg atgtggctca attttatgct ttctctctga aattggctca agaattaggt
    13201 tatatttatg gttatgagga tctttgggct tcacgcgagg agttgagtga agatgctcaa
    13261 aataccctct tgctttatct aggcgtaatg ttaggggtga atggaaccgc tgctttgcta
    13321 tgtgctggtg gtataacaat tgccaaacag gtaatgaaaa cagtgcctaa taaagcttta
    13381 acaaagacgc tttggtaccc tattttgaaa aaagtcttaa aaatatttgg tgtgaatctt
    13441 accaagggag ggctggccaa aggaataggg aaatttattc ctatcttggg tggtatcatt
    13501 gcaggtggtt taacctttgc aactatgaaa ccaatggggg aaagcttgca gaaagaatta
    13561 tccaagctag tcaactatag tgaagttcaa tatcaagaag atgttgaaac aatccgaaaa
    13621 gaggctgaaa tcattaaagg agagtaatat gaatcctatc aaagcttttg ataaaattta
    13681 tggtaattac tttttgaccg tgcaaggtgt aaaagtgatg aaaacgataa agaaagctga
    13741 ccatgtcgtt gttggtctgg gaaaactttt tattgcagat aagctaatgg atacggctcg
    13801 gtggctcatt aggccagagg agagagaatg aaattttttt tggtcttctt gctattcttt
    13861 ttatcaaacc gattattggg attgtgaaat tcttttggat gatcatttcc tttgcagttc
    13921 aattgctgtt ttacaagata ttgtttaaga tattggattg gctctttaaa cttatctaga
    13981 tggtaatcca agtcgcagag agctagcagg aactccactg ctagtttttt aatctctctc
    14041 catatggtat aatataagca gtaaaatcat tttatactct tcgaaaatct cttcaaacca
    14101 cgtcagcttc accttgcagt atatatgtt
//
LOCUS       DAVKFH010000042        14097 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKFH010000042 DAVKFH010000000
VERSION     DAVKFH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.042"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(276..1883)
                     /locus_tag="VW006_001469"
     CDS             complement(276..1883)
                     /locus_tag="VW006_001469"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9380300.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDTEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2273..2860)
                     /locus_tag="VW006_001470"
     CDS             complement(2273..2860)
                     /locus_tag="VW006_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9380301.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2988..3437)
                     /locus_tag="VW006_001471"
     CDS             complement(2988..3437)
                     /locus_tag="VW006_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380302.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3442..3831)
                     /locus_tag="VW006_001472"
     CDS             complement(3442..3831)
                     /locus_tag="VW006_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9380303.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3859..4485)
                     /locus_tag="VW006_001473"
     CDS             complement(3859..4485)
                     /locus_tag="VW006_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9380304.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4499..5062)
                     /locus_tag="VW006_001474"
     CDS             complement(4499..5062)
                     /locus_tag="VW006_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9380305.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5227..5461)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5515..6018)
                     /locus_tag="VW006_001475"
     CDS             complement(5515..6018)
                     /locus_tag="VW006_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9380306.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6211..7041)
                     /locus_tag="VW006_001476"
     CDS             complement(6211..7041)
                     /locus_tag="VW006_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9380307.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7026..8708)
                     /locus_tag="VW006_001477"
     CDS             complement(7026..8708)
                     /locus_tag="VW006_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380308.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8810..9067)
                     /locus_tag="VW006_001478"
     CDS             complement(8810..9067)
                     /locus_tag="VW006_001478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380309.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9074..9622)
                     /locus_tag="VW006_001479"
     CDS             complement(9074..9622)
                     /locus_tag="VW006_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9380310.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9637..10485)
                     /locus_tag="VW006_001480"
     CDS             complement(9637..10485)
                     /locus_tag="VW006_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9380311.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10673..12022
                     /gene="trkA"
                     /locus_tag="VW006_001481"
     CDS             10673..12022
                     /gene="trkA"
                     /locus_tag="VW006_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9380312.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12026..13465
                     /locus_tag="VW006_001482"
     CDS             12026..13465
                     /locus_tag="VW006_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9380313.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13590..13847)
                     /locus_tag="VW006_001483"
     CDS             complement(13590..13847)
                     /locus_tag="VW006_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEU9380314.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         4131 a         3212 c         2611 g         4143 t
ORIGIN      
        1 ctttccttat agagatgttc acatcttatt tcaattcatg atacaatcca gttcctaaaa
       61 ctttgctaga aattaatttg gctttccgaa ttaatttgtt cagattttat ttcatttcaa
      121 tatactgata aatccattca ctagcacagg gcaagaggga gtcgctattg atttcaacgg
      181 tagcaggtac atacagagga ctcctacctc agatatactg caatatcatc gtatgcaaaa
      241 aacctctgag atttttctca aaggttctga ttttgctaat tgctgttctc aaccgctgca
      301 gtaacaaagg cagtgtagag ttcttctggt cggtttggac ggcttgacag ttcagggtga
      361 tactgacaag ctacaaagaa tttattttca ggaatttcca cgatttctac caaacgattg
      421 tctggagaaa ctcctgaaaa gacaaaacct gctgcctcaa actgctcacg gaaggcatta
      481 ttaaactcat aacggtgacg gtgacggcgt tgcaccactt cttgattgtg ataagcagca
      541 gcagccttag agccacgttt caacttagac ggataaagtc ccaaacgaag ggttccaccc
      601 atatcctcaa tatcaatctg atcacgcatg atatcaatga tagggtattt tgtttctggt
      661 gcaagctctg cagaattggc accttcaaga cctaaaacgt gacgagcaaa ctcgatacat
      721 gtcaactgca ttcccaagca gactcccaac attggaacat cattttcacg cgcatagcgg
      781 atggcttgga ttttcccttc tgtaccacgt tgaccaaaac cacctggtac gatgatcccg
      841 tccgcatcag acaagagttc tgctacattc tctgctgtca catcattggc attgacccaa
      901 ttgattttaa cttctgtatc attgacatag ccagagtgtt tcaaggcttc gaccactgag
      961 atataggcat cttgcaactc cacatactta ccaacaaggg aaatcttaac ttgtttcttg
     1021 aggttcatga ccttgtccac catggctgac cattctgtca tagccgctgc tggtgcgtct
     1081 aatttcaaat gatcacaaac aatttggtcc atcccttgtg cctgcaagtt cagtggaatt
     1141 tggtaaaggt gttcaacatc caacgattcg ataacggctt ctggtgccac atcacagaac
     1201 tgggccagtt tatttttaat tccttgacca gctggctctt ctgtacgaat aaccaacata
     1261 tttggttgga ttcccaagcc acgcaattct ttgacagagt gttgggttgg tttggttttc
     1321 atttcaccag cagccttgag gtaaggaagc aaggttgtat ggatatacat gacattatcc
     1381 gcacccacat ctgccttcat ctgacgaaga gcctctagga atggcaagga ctcgatatct
     1441 cctactgttc caccaacctc tgtgataatg acatcagagt cggtcgttag agcggcacgc
     1501 ttgatttttt ctttcaaagc atctgtgata tgaggaatga cttgaacagt tgccccaagg
     1561 tattctccac ggcgttcttt acgaagaact tcactgtaaa ttttcccagt tgtcacgttg
     1621 gaatatttgt tgagattgat atcgataaaa cgttcatagt gacccaagtc caaatctgtc
     1681 tcagctccgt catctgtcac aaaaacttcc ccgtgctggt aaggactcat ggttcccgga
     1741 tcaatattga tataagggtc aaacttttga atggttactt tgagaccacg atttttcaag
     1801 agacggccta gactcgctgc cacaatccct ttcccaatag acgataccac accaccagtt
     1861 acaaaaatat atttcgtaga catagattcc tctttctaaa atgctcaagg tcttgttaac
     1921 tacgagggga tatagaaaac aagaccctac taataagaat aatctgggac gactgcacca
     1981 gattcacaac taatactctt cgaaaatctc ttcaaaccgc gtcaacgtcg ccttgccgta
     2041 gatatgtgtt cctgactttg tcagtcttat ctacaacctc aaaacagtgt tttgagcagc
     2101 ctacggctag tttcctagct tgctctttga ttttcattga gtataaaata caagaagata
     2161 acttcttgaa aaaacaaaaa tagctcccta agaactaggg agccccgacc tctaaaagag
     2221 gtgcccaaac aatatgatac ctaaaaatag gataattgtc aatagctaac tcttattcct
     2281 cgctggtttc agcgtcgtca tctgaaaact cgtcgtcgtc ttcgttgaga tccacatctt
     2341 ctcccaagtc atctggagcg atttcgttga tttctgcatc ataagcttcc acttcatttt
     2401 tttcatcatc tggattttca tcatcgtatg aaagagctgg atctgcttcg tatgcatctt
     2461 cgtcttctgg atcatctgca ttgtagtcaa tggcatctga atcaccatcc ataaaggcat
     2521 tgacacgttt tttcttagct tttggtgcta cttcatcgtc gtcattttct tcaagagcga
     2581 tgatttcttc gtcgatttcg tccacaccat accatgaacg aagaccccat ttgttgtccc
     2641 caagtgagat gaagctaccg tcaaagttca actctgtgta gaacagaggc aaagcttcgc
     2701 ggatatcgct gtttgatgtt ccaaggtagt tttgaatttc gtttacaaga tcgctaaaat
     2761 gcatctcatg atcgcgacca cgaagttcta atatagcacg cgctacctca atcatagata
     2821 gttcactttt ttcttgccca gcaaatactt ctaattccaa agcgtttctc ctcatttata
     2881 ctactatcgc cagagcgaac agactctgac ctcattttat catttactct ttattttacg
     2941 ataattttgc ggaatagtca aaggttaagg gggagaaagt ggcaggatta gactaattcc
     3001 aatataaaac tccttccttt ttctgttgct ccattttcca caaatccaag cgacttgaaa
     3061 cacctcctag aagcatgatt gtaggtgtag atttccttga ctctcaattc tttccatcct
     3121 tttactcgag ccaattcaat caaagcactt agaatctttt ttccaagtcc tcgatgttgg
     3181 taagcggaat tcccaatcac aatggggaga ttatcctgag atagtgtaat atccccaatt
     3241 ggaaaccatt ctcccttctc cttgacttca atccaaaaaa gctcaccatg ccgatccaaa
     3301 taggaataca tggcttccaa ggtcgcttga ctgtaaggaa gcttcacccc atctacgagg
     3361 taaaccaagt tcacatcctg ataccaagca aaagcttcct cacaatgatt gaccttataa
     3421 taaggaacaa aacacaatgc actacctttt caacaaaaga gttgctgctt gattaaaacc
     3481 atcacaccag ttataccatt ttgcttcata cccatcttga gctaggatac gatcttctaa
     3541 atcaaaaaca gagtaaatct ttctttcctc gcaagcttgc gcatagagat gatatagttc
     3601 atcaccacca tctctatccc actcagcaga aatcgtatcc cgatctgcca ataaagtctg
     3661 ataagccctg tgatgcccat ctgtaatcag caaacaatct ccaaaggcaa gaatactgat
     3721 tggatcgact tggattgttt ctgccgactg gtaaagcatc tgaatatctt gcaacttctt
     3781 ttctgataaa tatagttgag tcggatgaag atctgctatg ttgactttca tttctttctc
     3841 ctcaagggaa ttcgatactc acttctgttt gcctttaaat cgccattgga agcggagctt
     3901 gtcatagaag ggaaactcga taaacaggac tcccaagccc acacagagac tggcaaggac
     3961 gtctgatggg taatgaactc ccagatagac tcttgatacc agcacactga ctaggtagag
     4021 gccaaggacg atttgtacga tttttctcca gactggatct ttaatccgct gactaagaat
     4081 aacaatcaaa gtacctacca tcaaggttac agccagagaa tgcccacttg ggaaggaaaa
     4141 tcccttctcc tccaccagat gtaagatatc tggccgtggg cgctggtaga tatttttaaa
     4201 ggtcacgatt aaaagacctg ccaaagccag atttcccagc atgaagaaac tttctatctt
     4261 ccatcgctta cgataaaaga caaaagctgt aatgacaacc caagtgataa tcactgggat
     4321 atcaatcaga cgtgtgaggg ctcgaaaaag aatagtcaaa taatctggta agtctcctcg
     4381 aatggcagtc tgaatcgatt ggtcaaaatt gaccagcgtt tcagggtaaa atttgaccat
     4441 gtagccaaga ataacgaaaa gtaaaagggc aaaactgccc ttcattaaaa atgtttgttt
     4501 atctctcata atgttttaag gttggtttca agagaacata caacaaccag aatgaaacgg
     4561 aaaagataac accttcaatc aagttaaaag gtaataccat ggtcattagg tagttggaaa
     4621 gtcccaaaat ttttccaata tcaaagttag caaacttagc gtacaaagga acagcataaa
     4681 catagttgag aaccaacatg gccaaggtta aaccaatagt tccagctaga gagcctagta
     4741 ggaaacgaag ggttgtccgt tcctttttcc aaatcaaagc aaatacgatg acaaaaactc
     4801 ccaaagctac gatattcatc ggcaaaccaa tgtaagtatt cactccttgg ctgttaagaa
     4861 gcaacttcaa gagtgagcga agcaagagca ctcctagagc agcaggcaaa tccatgacca
     4921 ccagacccac aaggactggc aagatactaa attcgatctt gaggaaagat gccgctggta
     4981 aaagcggaaa atcaaagtac atcagcacaa atgagatggc tgatagaatt gcaatggtcg
     5041 aaagtcgacg tgtgtttgtc ataacaggtt cctccaattt tctataaaat cagaagaagt
     5101 tggaaaggat tcctctatct attctcactt tttatatccc aaaagttccc tcttactcta
     5161 ttaaagaaaa acaaagcaag tggttacaat ccggctataa atctatcaaa acagacaaag
     5221 ctattctttc gtcttctccc atccagacta tactgtcggt tgtggaatct caccacatca
     5281 gcttgcgctc gcggacttct ttaaagggaa agaaatagag atttttcttt tagactaggc
     5341 gacaagctgt aggcataact tctggttagg caaagcttgt caacaaagta taaaaggaaa
     5401 agatcatttc ctagttaaag tcaccgccgg tcgggaatca caccctgccc tgaagactct
     5461 tttatcataa caaaaaacgc ttgcaagcgc aagcattttg ttcattttca ttttttattt
     5521 caatttatcc acttcatagg tatggacgag ctcaagacct gcaaagttct gctggcgaag
     5581 agcctcatag acaatcatgc agacggtatt agacacattg agactgcgga catgttcatc
     5641 attcatggga atacggagag ccttctcagg atgttctcgc ataaaatctt caggtaagcc
     5701 cttgtcttca cgtccaaaga gaaaataatg gtcctcgtca gtcgataaat ccacctcaga
     5761 atagattttt tccgcgaatt tcgaaatcag atagagtttt cccttcatct gagatatgaa
     5821 atcttctaaa ctctcgtaaa aataaatctc tagcttatcc cagtaatcca atccagcccg
     5881 cttcatcttg cggtcatcaa taggaaagcc cataggcttg atgatgtgga ggggagaatt
     5941 ggtcgcagcg caagtacgcg cgatattgcc tgtattttgt ggaatttgag gttcaaataa
     6001 tacaatgtga tttgtcatga cttgcttcct ttcaccattg caaaaaaata gccacactgc
     6061 ccggagtcaa gctcagcaaa cagcgtggtt aaggcatcgt taacttacct cacaacaggt
     6121 ttgaagtaaa tcagcgaaac tactttctta gtataacact ttcagaatca ttgtcaatag
     6181 aaatgacttg attttttcaa ttttttcaag ctatttccaa gggttgtaaa atcgtccctg
     6241 attctgcaag ataagtagta aactaactac taaaaacaag gttgccaaga gcaaggtaat
     6301 atagtctcct tttttcaagg cctgataact ataccatgtg cgttttttct ctttcccaaa
     6361 gcggcgaagc tccatggcag tcgcaatggt atcaatgcgt tctagcgagc taaaaatcaa
     6421 gggcgtaata atgagcagat tgcctttgat tcgttgcata agagaagctt tcttggataa
     6481 ttccatccca cgcgcctcct gagacatctt gatagtaaag aattcttcct gcaaatctgg
     6541 aatatagcgc aaggtcaggc tgacagaata agcaatctta tagggcacac caatttgatt
     6601 taaactggaa gcaaactgac taggatgggt tgtcatcaaa aagataatag ccagaggaat
     6661 ggtgcaaaga tacttaatgg ccaaatttag cagataaaag agctcctggc tggttagagt
     6721 gtagacaccg attccctgcc aaatcacact tctctctcca taaagtccaa ccccatactc
     6781 gggagaaaag agatagacca tcaaaacgtt taaaacggca aataccgtcg caaaaacggc
     6841 tacaaaggaa acatctttaa agcgaatttc tgataaatag aggagaaaga ctgaaaagat
     6901 ggcaatcagc aagagcattc tggtatcata gctaatcatg gccgccaatg ataccagaat
     6961 gaaaaagaga agtttcccag ctcctgacaa gcgatgaatc acagtatctc tatgctggta
     7021 accgattaat ttagcttgca tccctctctc ctttctttgt aaaatgccgt taaatccagt
     7081 ggatccacat ctagtttctt agccaagtta aagatggagg tttcttttag attggctttt
     7141 actaacagct caggatcgct caacagactg gctggaacag tatcggcaat caattctcca
     7201 tccaccatga caaggacccg gtctgaataa tccagcatca attgcatatc atgggtaatc
     7261 atgacaatgg tatgcccttt ttgatgtaac tcttcgagaa attccataat ctcagtatag
     7321 ttcttctgat cttgacctgc agtcggttca tctaggagaa taatttcagc tcctaagacc
     7381 aaaattgaag caatggtgac acgttttttc tgaccaaatg acagggcaga aataggccaa
     7441 ttacggaatt cataaagtcc acagattttc aaggtttcat agactctcgt ttcaatttcc
     7501 ttctcatcca cacctcgcaa acggagccct agagccacct catcaaaaat catattggtt
     7561 gaaatcattt gattaggatt ttgtagcaca tagcctactc gttccgcccg ctctgcaaca
     7621 gaatcgcctt ttatatcctg tttttcccaa agatagcgtc cttccgtctg aataaagcta
     7681 cttatagcct tggctagagt tgatttccct gctccatttt ttccgacaat agcaatcttt
     7741 tcaccctttt taatatctaa atgtagggat tttaaaatcg gtctatcatc ataagagaaa
     7801 gacacgtcct ctaatttaaa gagtgactgc aattctggag tttcttttgc cagttcattc
     7861 tgcagctgaa cctgaccttt tgagatagac atgttatcca gattcgctaa ctgttcttcc
     7921 ttgactaagt ccacacctaa ttgacggaga gtcgttagat aaaggggttc tcgaattcca
     7981 ttttgagtca ataaatcagt cgcaagcaac tggtcagggc tcccattaaa aaggatacga
     8041 ccatcgctta tcaagacaat ccgatccaca gggcgatgca gaacatcctc caaacggtgc
     8101 tcgataatca gagtcgtcgt cccctcttcc ttatgaatct ggtcaatcaa ttcgataata
     8161 tcctgacctg acttgggatc tagattggcg agtggctcat caaacaagag aatcggactt
     8221 tcatcaatca agacaccagc cagactgact cgctgctttt gtccacctga caaatcctga
     8281 ggacgctgat ccagtaaagg aagaaggtcc agcttttcag cccatttata aacacgacct
     8341 ttcatctcat ctagggctgt cacatcattt tccagagcaa acgccaaatc ttctgccaca
     8401 gacaagccaa taaactgccc atctgtatcc tgcaaaactg tgctaaccag atgagactta
     8461 tcatagatgc tcatatcaaa ggctacttga ccctttatca aaaattctcc atatgtctga
     8521 cccttgtaaa tattgggaat aatcccattc aaacactgac ccaaggtaga tttacctgac
     8581 ccagatggtc caacaattaa gactttctct cccttgtaaa tggtcaagtc tatcccttgc
     8641 aaggtcggtt cttgttgtgt ttcataccgg aaagagaaat ccttccactc aattatagct
     8701 tctttcatct tactctcttc attcgcttct tagacttcta ttttatcata aatcaagccc
     8761 ttcttgcagt ctcttctctt aaaatcttag cgccaaaaag attcctatcc tagcttactt
     8821 gcctaactaa tctataaaca tcgaaaaaga ctagttgccc agccttcccc atcattttat
     8881 actcttcgaa aatctcttca aaccacgtca gcttcgcctt gccgtaggta tggttactga
     8941 cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg tcagttctat
     9001 ccacaatctc aaaacactgt tttgagcaac ctgcggctaa cttcctagtt tgctctttga
     9061 ttttcattga gtattagtcc tttttcaaac ttcctgcacg agtttgggtt cctgcatagg
     9121 caagtaagag aagagttcct gcaatagcta cagatacacc attggcaatt cccgcaacaa
     9181 tcccttgtgc aaatactttt tctgccgctt cttgataaat cacaacatct ccaagtggtg
     9241 ccaagacacc ccaaacaagg gcatttgcaa gtagttgaat gaggttaaaa ataagaatat
     9301 ctttccagtc aaaaacacca ttgatcactc gaacgtactt tctaaaaagt cccacaacta
     9361 gaccaaagag tccgctagcg ataatccaag tccaccatag accataacca gcaagagagt
     9421 ccttgattgc atgaccaatc aacccgacaa gcaaaccgat aatcggtcca aaaataatag
     9481 aaagtagcgc ttgtaccgca tactgaagct ggatgcttgt atttggaaca ggggttggaa
     9541 tgttgatcat cccgatgacg acaaagaggg cagcgccaat tccgacagca acaacttgtt
     9601 taattgtaaa tttgatttcc atactattct cctattttat ccttctattt tctttatttc
     9661 aatggtccaa gatgaaccga cacctacatt ataggccttg gcaaaggaac cttggttgat
     9721 agccaaacct aaacgataga gagagttgat gtaaaggatg ggttgcccaa ttctcacatc
     9781 tgcaaatgat ttgccataga caacctgatt ttgatagacc agcatatcag catgatagat
     9841 ggtcacttca aaacgatcac caaattctgg ttccagcttg taaaattctt cccgtgtgat
     9901 agaggtccaa agcgaaccga aacgcacatc cagaatatca atggctccct tcaccagatg
     9961 atcttctatg atggtcgcta cgactggaag ctctacaatc tgttccacac tgagctctgg
    10021 ccctacttcc tcaaaagtaa tgtgaccact ggccagttta gcaccagtat aggcatagac
    10081 atcacgaccg tggaaggtat aagaatgctc tgtgttttga cgcctattgg ccacctcaga
    10141 aatctcacga atggctacaa tgccaacgtg tttcttgata aaggaaagcg tcccattatc
    10201 tggcgtgaca atgtattgat tttttgcagt cttggcaact acactcttac gtttcgaacc
    10261 gacacctgga tcgacaaccg atacaaacgt cgttccctca ggccagtaat ccaccgtctg
    10321 aaagagacga tagctcccct caaaaatatt ataaggcgtg atatcgtgcg tcaagtgatg
    10381 gatttttaag gttggagact cttctaaagc cactccaatc atagccgaca ccgcaccatc
    10441 aaccagacca aagtctgatt gtaataccag taaattattg ttcattttat ctccttgtat
    10501 atcctttatc ataccatatt tcagagaaag tcgctaatag ctatttcaat tggttagggt
    10561 atagttttac cttatagcga attttcacta aaagctcttg ttattagcta gtaggtgcat
    10621 tttttcttga aaaagggtat gatagttaca tcatgaaaaa gtaggtttta ttatgaaaat
    10681 tatccttgtc ggagggggaa aagttggttt tgccctctgt cgctccttgg ttgcagaaaa
    10741 gcatgatgtt ttgctgattg agcaagacga agctgttctc aatcatattg tcagtcgctt
    10801 tgatatcatt ggtatccttg gtaacggggc cgattttgcc attcttgagc aagccagcgt
    10861 ccaagattgt gatatcttta tcgccctgac tgagcacgat gaagtcaaca tgattgcagc
    10921 agttctagcc aagaaaatgg gagctaaaga aactatcgtt cgggtgcgga accctgaata
    10981 ttctaactct tatttcaagg aaaagaatat tctcggtttt tctcttatcg ttaatcctga
    11041 gctcttggct gcccgcgcta tcgcgaatat cattgacttc cccaacgccc tgtctgtcga
    11101 acgctttgct ggtggacgcg ttagcctcat ggaatttgtc gtcaagtcca ccagcggtct
    11161 ttgccaaatg cccatttctg attttcgtaa aaaatttggt aatgtcattg tctgtgcgat
    11221 agagagggat catcaaatta tcattccaag cggtgacatg actgtacagg ataaagatag
    11281 aatctttgtc actggtaacc gtgtcgatat gatactcttc cataattatt ttaaatcacg
    11341 cgccgtgaag agccttctca tcgttggagc aggtagaatt gcctattatc tacttggtat
    11401 tctcaaagat agtcgtatcg atacaaaagt cattgaaatc aatcctgaaa tcgccagctt
    11461 ctttagcgag aaattcccaa atctctacat cgttcaagga gatggaaccg caaaagatat
    11521 cctgctggaa gaaagtgctc aacactatga tgccgttgcg actctaacag gagtcgatga
    11581 ggaaaatctg attacatcta tgttccttga cagggtaggt gtacagaaaa atattactaa
    11641 ggtcaatcgt accagtctcc tcgagattat caatgcgcct gatttttcaa gtatcatcac
    11701 acctaaaagc atcgctgtag atacgattat gcactttatt cgtggtcgag ttaatgccca
    11761 gtattcagac cttcaagcca tgcaccatct agccaatggc caaatcgaaa ccctgcaatt
    11821 ccatatcaag gaagccaata aaatgactgc caaacctctt tctcaactga aattgaaaaa
    11881 aggggttctt attgcagcca ttattcgaaa gggcaagact attttcccaa ctggggagga
    11941 tatgttggaa gttggagaca agctcctagt aacaaccttg ttgccaaaca tcaccaagat
    12001 ttatgacttg attgcgaggt aagaaatgaa taaaagtatg attcgttacc tcctttcaaa
    12061 gttacttttg attgaagctg ttctcctctt ggttcctgtg gctatcgctg tctattaccg
    12121 tgaatcgagc caagtcttta cagccctctt ttcgaccata gggattctcg tattgttagg
    12181 cggttcaggg agtttacaga agccaaaaaa tcaacggatt tatgccaagg agggagtctt
    12241 gatcgttgcc ctctgttgga tcctttggtc tttctttggt ggtctcccct ttgtcttttc
    12301 tgggcaaatc cctagcctta ttgatgcctt ttttgaaatt agttctgggt ttacaactac
    12361 tggagcaagt attttgaacg acatttcggt tctcagccgt tccctcctct tctggcgaag
    12421 ttttacccac ttgattggag ggatgggagt gcttgttttt gcacttgcta ttatggacaa
    12481 tgccaaaaat agtcacctag aagtaatgaa ggctgaggtt cctggtcctg tctttggtaa
    12541 ggttgtatcc aaattaaaaa acactgccca gatcctctat ctcctttaca tagccctctt
    12601 ttccctcttt gtcatcatct attatctagc cggtatgcct ctctacgata gttttgtcat
    12661 tgctatgggg acagcgggaa ctggaggatt taccgtctat aacgatggaa ttgcccacta
    12721 tggtagctca ctgattacct atctggttag tataggagtt ttggtttttg gggtaaattt
    12781 caatctctac tactacctta tgctccgtcg ggttaaggcc ttctttggag atgaagagct
    12841 tcgggcttac ttggtcatcg tgctggtttc tacaggcttg attagcctca atactctcta
    12901 cctctaccca gggttttcaa agagctttga aatgaccttc ttccaggttt ccaacatcat
    12961 tacaacaact ggttttggat atggagatat taccaactgg cccctcttct cccagtttat
    13021 cctccttttc ctcatggcaa tcggtggctc tgctggatca actgcaggtg gactcaagat
    13081 tattcgaggc ctcatccttt caaaaattgc caaaaaccaa attttgtcaa tcctatcgcc
    13141 ccaccgtgtt ttgaccctcc atgttaataa aatggtgatt gacaaagata cccagcataa
    13201 gattctcaag tactttgtca tctatgctat gattttgcta gcgcttatct ttattgtcag
    13261 cctagatagc aatgattttc tggtcgtgac cagcgctgtc tttagctgtt tcaataatat
    13321 cgggcctatt ctaggaacca cttctagttt ctcaatcttt agtcccatct caaaaattct
    13381 cctctccttt gcaatgattg cagggcgctt ggagatttac ccaatcctcc ttctctttat
    13441 gaaaagaact tggtctaaga gataaaatac aaaatccctc tttttacaat tgtgaaagag
    13501 ggatttttat attaaaaagg gggaacgttt ttaaatccaa gcctgtctgt aagcaaagtt
    13561 cttactcaaa taaaagatgg aaaaacttac tacacagctt ggtaatgctc caatacaaat
    13621 tgtaaggctt tacttggatt ctttgttaaa tgaatatatt ccattcctct ggtagcaact
    13681 atctgaatac ctaatctttc tgcatccacc ttcatctctc tataataact ccgaacttgt
    13741 ggagccagaa taattaaatc ataaacaccc ataatatcat aatgagctcc gtacgctcct
    13801 gaattcgcaa tcactctaac ttctgctaat tgagcgccct cgttaattgc attggctaat
    13861 tacgcacttg ttccagaccc tgcacaaaga accagtactt tcaactcctt actttttatt
    13921 acatctatct caccaggcat tatttcactt gtatcctcct ctaaaataat atcatttgag
    13981 cttgcaatat ctacaacctg tagtagatat ctcgtattta gacaatatga aaacaagacg
    14041 acttccatat aggaaaccgc cttctctcta tgttgagtga tttatattaa aataact
//
LOCUS       DAVKFH010000043        14046 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKFH010000043 DAVKFH010000000
VERSION     DAVKFH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.043"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(136..2694)
                     /locus_tag="VW006_001484"
     CDS             complement(136..2694)
                     /locus_tag="VW006_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9380315.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            3242..4442
                     /locus_tag="VW006_001485"
                     /pseudo
     CDS             3242..4442
                     /locus_tag="VW006_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4557..5360)
                     /locus_tag="VW006_001486"
     CDS             complement(4557..5360)
                     /locus_tag="VW006_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9380316.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(5643..6398)
                     /locus_tag="VW006_001487"
     CDS             complement(5643..6398)
                     /locus_tag="VW006_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9380317.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPCFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(6468..9119)
                     /locus_tag="VW006_001488"
     CDS             complement(6468..9119)
                     /locus_tag="VW006_001488"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9380318.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGIDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(9131..10093)
                     /locus_tag="VW006_001489"
     CDS             complement(9131..10093)
                     /locus_tag="VW006_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9380319.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
     gene            complement(10095..10652)
                     /locus_tag="VW006_001490"
     CDS             complement(10095..10652)
                     /locus_tag="VW006_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380320.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            complement(10639..11001)
                     /locus_tag="VW006_001491"
     CDS             complement(10639..11001)
                     /locus_tag="VW006_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380321.1"
                     /translation="MLAMEQAGFISHQEKLERLGVHYEISERTQTPILDACPLVLDCR
                     VDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFMGDGYQRVYRYLDKR
                     VDMKGSFIKKARKKDGKN"
     gene            complement(11501..11896)
                     /locus_tag="VW006_001492"
     CDS             complement(11501..11896)
                     /locus_tag="VW006_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9380322.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            complement(11992..12246)
                     /locus_tag="VW006_001493"
     CDS             complement(11992..12246)
                     /locus_tag="VW006_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9380323.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(12256..13590)
                     /locus_tag="VW006_001494"
     CDS             complement(12256..13590)
                     /locus_tag="VW006_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9380324.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(13590..13823)
                     /locus_tag="VW006_001495"
     CDS             complement(13590..13823)
                     /locus_tag="VW006_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9380325.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            13854..13931
                     /locus_tag="VW006_001496"
                     /pseudo
     CDS             13854..13931
                     /locus_tag="VW006_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT         4197 a         3108 c         2469 g         4272 t
ORIGIN      
        1 tttcatttta agatatttca atctatttgt caagtacttg tagaaatttt tttattttgt
       61 gtgcttcgaa tgtgataatg tcaacagtct cattaaaaaa tgaaaatcat acatagaaat
      121 ttttaaaaat tttctctatc gtaaacctgc cactagttca gaaactatac tagctgattc
      181 caaacgtaat atggtcatct catctacctt ccctaataat ggcattgcac cataccaaac
      241 aatagtctta ttcaccaaaa gaataggcgt tactattttt gattgagtaa aagacacttt
      301 aattttctgg ctcttcaact ggtctagcca tgctttattt gcaaccgtct ctggtataca
      361 tatctcaatt tgactaatcc taaattcttt tagaaaattc atcagttttg tctggtgcac
      421 ataaggtaaa ataagcagac attcctgtct ttcccctgct aagtcctctc tcaacacctt
      481 ctcatatcta ctatcaacat aaacgaattg tttctcctca ccctcgatga cacgataatc
      541 catctttcga taagctactt gtcgtttctg aaacatcttt tctaaataag gaacatgaat
      601 atccacataa tcgaaaatac gcaccaaaga cttatccttg tagtttctat gaatccgacc
      661 tgcatactga attaaattat ttttccaaga aaagggtgct gccaagataa gcgtgtccaa
      721 ctgaggtaag tcaaaacctt cgccaatgta ttttccagta gacaacaaaa caaacccttt
      781 atctaactgt tctaacgtct ccagtaaact cgttctttct cggactttgg tttttccgct
      841 aataatgtaa cagccatcaa cctctttctc tttcaataac ttttcaaaga catctatctg
      901 ttgaattcga ttaactaaaa ccaagatatt ccgtccttct gccacttggg ctagaatatc
      961 cttgagaatc aactgattcc tcgctgagtc tgtagcaatc caatcactga gctgtataaa
     1021 attacttgct ttggtctttt caatctccaa atgaccaaaa gaagtgaatc ttaattgcaa
     1081 ttgccgttta aaatccgttt ccctcttatc agcagtatgg agtatctcac caattctctg
     1141 aaaaacaata ggctcatgac cattcttacg ctcaggcgta gccgtcaaac cgtaaagata
     1201 cttccctcta aactgagcaa caactttttc aaacatcaag gcagagacat gatgacactc
     1261 atccacaatc atcatctcat actcatccaa aagactttga ctattttcca acttaaatag
     1321 agattgaatc ataacgacat caaccagttt actcagccat ttcttagtcc caccgtactg
     1381 tccaacatag ccgattacct tttcacgacc tgatgccgta taacggatag cctcctcctc
     1441 ttcgaaagtc aaaaagcagt ttaagcgatc tagccattgg tctaagagtt gcttattatg
     1501 gactagaata attgtttttg ttttccgttc agagataaga gcagcaccta aaacggtctt
     1561 cccaaaacca gtctccgcat gaagtaaacc attttcttta gaagtcatat ctgacagggc
     1621 taactcttgc tcaaaagtga gttctccctt aaattccacg ctaatagacc tttgtacctt
     1681 tctcctatct tccacagata cctgcttaaa tttatcttgc aatggataca gcaaacctct
     1741 tggcaaccat aaataatgat cggattctcc aaataaatac attctctcag gaatttgata
     1801 ggttggctgt cgcatagcct gctttaaata aaattcggga ttagaaaatg aagccatatt
     1861 tttcaagaaa aacagtgtct tgggtgtcac agacgatttt tcaagttgga tcatattgga
     1921 taagacgatc ttcaactcct tgtccaattc ttgcttgcct aactcctctt ggattaacag
     1981 tgccacttta gcagttgaaa tcttctgaat ttcttgtaga tacctccatt gatcttcata
     2041 aggctgaaac tgttcatcca caaagaccgt tcgcccttga tgataagctt ctccttgaaa
     2101 aggaagggaa attaaattat caaatccacc tttaggaaga acatcctgat ttggaaacat
     2161 gcgatcaaaa gaatcaaagg acagttgcat actttcctgc atagccaatt ctaatagttt
     2221 ctttccaaac aggcgagcct ttcgactcag aatcgcttcc tcaaagaaga accaaatatg
     2281 gagtccgtaa cccgaacgag aaatctctaa atgggcttcc atctggcgtt ccctggctat
     2341 tcttcgaatg gttaaaccag cttctttcca atctccttca tccaaatcca gtaccaaaaa
     2401 ataacagcta tcatctaagt gcataggaaa gataccgata gctgcctccc cacgaaaatg
     2461 agatttcaaa acggagttcg tcaatgtctg gaaactccgt ttttcaggtg gcaactgctt
     2521 ccaaccataa tcataggaag gaaaatactg aattttccct tgttcattga taaaattctt
     2581 agcataaaca tcataacggc cagcaaacac agaggtaaac aattgtaatt tttcttgcgt
     2641 cgtcaagcga ctactttgaa gttggaacat ctcctcgaac tgagagcgtt ccataacaaa
     2701 ctgactcttg tctttaagac gtttatagtg gaatgaaata agatatgaac aaattgatta
     2761 ggaaaatcaa attaatttat agaaatcttt tagcagtcat ggcgtcctat tccagattca
     2821 aaatgctata cacaagaaaa actcactatc cccattaaaa gaagaaaaga catcaaggac
     2881 ttctaccaca attccatcca aagacacaaa aacagccata agagtcacct ccttgattcc
     2941 tataggctga ttataacaag actggctgaa attgtacatg aaaataaaat cctaatagta
     3001 ctcattttgt atgtgactaa tattccgtct cgctccagaa ggtacgaagt aaatagagtt
     3061 taaacgagca gtctataaaa ttcaaaaaac gcatattatc aagcgaccta aaactttggg
     3121 ctctataata tttgtagtgg gtaaattccc tatggatatt atggagccta ttttgggtag
     3181 aaaaaaagtc ccataagatc tataatgaaa agcgacaaaa caactcatta gaaagaatca
     3241 tatggaacaa ttacatttta tcacaaaatt actagacatt aaagacccta taaaattcct
     3301 taccttgaaa cgactggtat gcctactaga attctcctta gaaaacgtcg ttttaagtgc
     3361 tatcattgct tgaaaatgat ggtcgctgaa acttctatca tcaagaaaaa tcaccaaatt
     3421 cctcgtatta tcaaccaaaa gattgcgcaa aagttaattg aaaagatttc tatgaccgat
     3481 attgcccatc agctggccat ttcaacttca actgtcattc gaaagctcaa tgacttccac
     3541 tttgagtgta attttagaaa tctgcctaag attatgtctt gggacgttga aacagtccgg
     3601 ggagtgactg tttcaatcgg gagatggaga tgagctttat tgcgcaagat tttgaaaagc
     3661 tcgatatcat cactgttctt gagggtagaa cacaagctgt catccgagat cactttctta
     3721 aatatgatag agccgtccga tgtcgcgtca aaattattac tatggatatg tttagtcctt
     3781 actatgactt agctaaacag cttcgctttc aaatttctag gctcaggctg aaacagtctc
     3841 ccagactgtt tcactcccga atgctaaaat cgttcttgat cgctttcata ttgtacaaca
     3901 tcttagccgt gctatgagtc gtgtgcgtgt ccaaatcatg aatcagtttc atcgaaaatc
     3961 ccatgaatac aaggctatca agcgctactg gaaactcatt caacaggata gccgtaaact
     4021 cagcgataaa catttttatc gccctacttt tcgtatgcat ttaaccaata aagagatttt
     4081 agacaagctt ttgagctatt cacaagactt gaaacatcac tatcagctct atcaactctt
     4141 gctgtttcac tttcagaata aggaaccgga gaaatttttc gaacttatcg aggacaatct
     4201 taagcaggtt catcctattt ttcagactgt ctttaaaacc ttcctcaaag ataaagaaaa
     4261 ggttatcaac gcccttcaac tacactattc taatgccaaa ctggaagcga ccaataatct
     4321 catcaaactt atcaagcgca atgcctttgg ttttcgaaac tttgaaaact tcaaaaaaac
     4381 ggatttttat cgctctgaac atcaaaaaag aaaggacgaa atttgtcctt tctcgaactt
     4441 agcttttctt caacccacta caattgacaa agagccaaac tttgatgata tacgttttct
     4501 tgtgagaata tttacttcat ttttgcctaa aattcaatgt ttactcagta tttggattat
     4561 gaaaaatcaa ggtctaaatc tagatacatt ttttctgaag acaaatcatt ttgaccaccg
     4621 agcaagagat tttcaaaaaa agctgttaaa aactcaggac gtcgctgtaa aatctttgca
     4681 ttatctaata ccaaggcatc acgaaaatat ttggaatgtt gctgaaatgg tgtattatca
     4741 atatcaaaac caaactcacg aagatactga atcaaaaaga ccgttactgt ccgagtgttt
     4801 ccttcgcgaa atggatgaat ctgccagatt cctgaaataa aatgctggat ttgtttaacc
     4861 acatccgcct gagttagtgt cgcatatgca acttgttttt cctgattaaa atcataatct
     4921 aaggtcattt gaatcatgga gtaatcagag tacacaacac tttcaccatt caaaacaggt
     4981 tcattctttg tgatattggt ctgacgaaat tgccccaccg gaatagaggg ttcaaatata
     5041 tcttgaaaca actccttatg aatagcaagt aaggtcgcag gactaaagct aaagcctctt
     5101 cgagacaata gttctacaat acgtagagaa accaagtctg cctcttccgt acttgcatca
     5161 atagtatggt gataagccgt tgcatcctca taaacctgct cataagtcag ttctccccgg
     5221 gactgtttct cagccaaaga ttccatatac gctgatggca ctagattgtc aactttctgc
     5281 agaccaaaac ctatccgcca taaatcacgc ttcgcttcat aagacaagtt tggattgtca
     5341 atgttgtaag ttggttgcat agaaatatcc tttcaattgt ttttcttcat taccagtaaa
     5401 tcacgaaatt ggttggaaac tggcttaaac tataaaaagt cctcacaata ggttctatga
     5461 tatatatata tatatatact tcatgtaaat aataaaagag ttataatcag cactactgat
     5521 aaattcaata ccgatttcgc catcttaaaa cttagtcaaa cgaataccgt tgatttccag
     5581 ttcattaagt cgttgacctg tcaagacttc tcgttccttc aactggaaaa gttaaggggg
     5641 aactaataca aatcctctac gcttctatat tcaatgattt catttttgat aatattcttt
     5701 ttaaactcaa aatgttttaa aggattatcg attaacacta actttggaat atcatctaga
     5761 tttgtgacca aggataaaat aaaatccgat tgaaactcct tgactttttc aaattctttt
     5821 gcagttaaac gcacacgccc cttagatttt cggatacctt ttacttcggc aaggtaagaa
     5881 tactcttgga catctacctg aaagtcatat ccatcaccat aaagacgggc atcggttaac
     5941 tgtccacctt ggaatttttc ttctttatca aaatgaagga taaaataatt ctcagcctcc
     6001 attcccgttt cctgtagacg tttgaattta gttcgaacta ttggtttttc aacgatggtt
     6061 atgcctgttt tcttcccttc actggcaagt aacattttaa ctatttcagc ataactatga
     6121 acatcttcat ttccaaacat catatcaatt aaatctttac gaaaacgata aacttcagct
     6181 ttctgccacc aaccttttcg attattgtca aaacaaggat caaataaatc cattctattt
     6241 tttacaactc cagttgtttc agcaattccc agagaaacca catagcgata aaactccgac
     6301 ttgctaaagc attgaaattc ttttataaaa agcttgtcaa acttggcaag accgtagcca
     6361 attaaattta acaattcata atgagggtgt ttggacatca ctttctccat caatagatat
     6421 tgatttcatt ataccaaaaa acacggctca tcaccgtgtt tctgtgttta tttcaccaac
     6481 ttcttcatct catcaatacg tgctacggtc acatcatact tggcttggta gtcggcttgt
     6541 ttgtcttttt ctttttggac aacttctggt ttagcgttgg cgacgaagcg ttcgttagag
     6601 agtttcttac caaccatgtc cagttctttt tgccatttag cgagttcttt gtcgagacga
     6661 gctagttctt cttcaacatt gaggagatcg gccagtggca agaagatttc ggctcctgta
     6721 ataacgcttg acatagccag ttcaggtgca gggatggttg atgcgatttc caagtgttct
     6781 ggatttgtaa agcgtttgat atagttgaca ttgctgttaa agaaggcttc caagtcacta
     6841 tcgcttgtct taacaaggat ggtgataggc ttgcttggtg ctacatttac ttccgcacgc
     6901 gcattccgaa cagcacgaat taagtctttg agactttcca caccagtgtg agccgcaagg
     6961 tcttcaaagg ctagattaac agttgggtat gcagctgtca cgatagaacc ttctgagatt
     7021 tgtccaaaga tttcctctgt cacgaatggc atgattgggt gaaggagacg aaggatcttg
     7081 tccaaagtgt atagtagaac agaacgtgtg atgactttct cttcttcgtt atcgctataa
     7141 aggacttcct tggtcaactc aacataccag ttggcaaatt cttcccaaat gaagttgtaa
     7201 aggatatgac cagccacacc aaactcgaac ttatcaaagt tttcagtaac ttttgcaatg
     7261 gtttcgttga gattgtggag aatccagcgg tccgtcacat taccagcctc acctgttgca
     7321 acttttgtga cattgtcatg cgccacatcc agcgtcaaac ctccattgtt catgaggata
     7381 tagcgagaaa tgttccaaat cttgttaata aagttccatg aagcatccat tttctcataa
     7441 gagaaacgca cgtcttgtcc tggcgcagaa ccgtttgaaa ggaaccatcg aagggcatcg
     7501 gcaccgtatt tctcgataac atccattggg tcaatcccgt taccgagaga cttagacatc
     7561 ttgcgtcctt gctcgtcacg aatgagaccg tggataagga cgttttggaa tggttgacgg
     7621 cctgtgaatt ccaatgactg gaagatcata cgagacaccc agaagaagat gatgtcgtaa
     7681 cctgttacca aggttgaagt tgggaaataa cgtttaaagt cttctgagtc gacttcaggc
     7741 cagcccatgg ttgaaaatgg ccagagggca gaactgaacc aagtatccaa gacgtcttcg
     7801 tcctgagtcc atccgtcacc ttctggagct tcttcgccga catacatttc accatcagca
     7861 ttgtaccagg cagggatttg gtgaccccac caaagctgac gagagataac ccagtcgtgg
     7921 acattttcca tccattgaag gaaggtatcg ttgaaacgag gtgggtagaa ttcgaccttg
     7981 tcctctgtgt cttggttggc aatggcgttc ttagccaatt ggtccatctt gacgaaccat
     8041 tgagtagaca agcgtggctc aactacgaca cctgtccgtt ctgagtgacc gactgaatgg
     8101 acacgttttt cgattttgac aagggcaccg atttcttcca acttagcaac gactgcctta
     8161 cgagcttcaa aacgatccat gcctgaaaat tcaaaggcaa gctcattcat agttccgtcg
     8221 tcgttcatga cgttgacttg tggcaagtta tgacgttggc caaccaagaa gtcatttgga
     8281 tcgtgggcag gtgtgatttt cacgacacca gtaccaaact caggatctgc gtgctcatct
     8341 ccaacgattg ggatgagttt attagcgatt ggaaggatga cgtttttacc aatcaagtcc
     8401 ttgtagcgcg ggtcttctgg attaaccgca accgcaacgt ccccaaacat agtctcagga
     8461 cgagttgtag caacttcaag ggcgcgtgaa ccatcttcca gcatgtaatt catgtggtag
     8521 aaggcacctt ctacatcctt gtgaatcacc tcaatatcag aaagggctgt gcgagctgct
     8581 gggtcccagt tgatgataaa ctcaccacga tagatccagc ctttcttgta aaggtccaca
     8641 aagaccttac gaacagcttt tgacaaacct tcgtcaagag tgaaacgctc acgagaatag
     8701 tctacagaaa gccccatctt gccccattgt tccttgatgg tagtggcata ttcgtctttc
     8761 cattcccaga ccttcgtcaa gaaagactca cgacctaggt cataacgcgt aataccctca
     8821 ccacgtaagc gctcctcaac cttagcctga gtcgcaatac cagcgtggtc tatacctgga
     8881 agccaaaggg tatcaaagcc ctgcatgcgt ttttgacgga tgatgatatc ctgcaaagtc
     8941 gtatcccaag cgtgaccaag gtgaagtttc ccagttacgt ttggtggtgg aatcacgatt
     9001 gaataaggct tagccttttg atcgcctgaa ggcttgaaaa catccgcatc aagccatttt
     9061 tggtaacgac cagcctcaac ctcggctgga ttgtatttag atgaaagttc tttagacatg
     9121 tgtgttctcc ttaattactt ttattgacaa ttttaaccaa accaattttt gaaagttcaa
     9181 tataaatctc ttcaatttca gcaaatgctg caagtggtga catagtataa tttccaggat
     9241 tatatttaag atcatacttg cttatctttg aaatataata cctagagtgt ttcaatttta
     9301 atgattccaa ctttgattta atatctacta atgtattatg gtttaacttt aaaaacaact
     9361 tattataatt accatagagc tctgtaagag cattaaagta ttctttcact agagctatag
     9421 cgtcaatctt agatgtttct tggtttagtt ttatcaactc ccctttcaat tttttccatt
     9481 taaagttgga atttaaaaga taatctaaat caatatagaa atctattata acagtctcat
     9541 catctgggct aacagctttt acctcattta ttggtaagtc agtatgttgc gaataatttc
     9601 tcaaagaatg gcaaaatcga taagcaaaat tggtatcata ctgatagctt gcaaaaccat
     9661 gtatacaatc aaactcctca ctatctttgg aatatttttg tttaatttgg ttttcgttaa
     9721 aatcgacaaa caattttcca ctggataata aatgaatcat gaaacgattt acatctgcct
     9781 cttcaatttc taattccttt gactcaagtt tatttttaat acttaaaaaa tccgagacat
     9841 ctgaaataaa caattcgaac atattcttga ttttttgaaa attcttttga atctcaaaaa
     9901 tttggtccga gactgacatt atttccttgg ttggttcaat atcagagatg acaatacgtt
     9961 tttcattgtt gttaatttct aaataagcta atcgctcgat aatatcacta tcatttaatt
    10021 cagcatactc aacatcaatt tggcgtaact gcttctcaga tagagagctt acttcctctt
    10081 ttacatcgct catcttattc ctcccactcg ctcttcagca agccatatat cagactgtca
    10141 cagcggtttc cttgaacatc ctttcggtct cttatgcgag cttcgagggt aaagccaagc
    10201 ttttccgcga ctcgttgact ttgaaggtta tatccaaagc aagttagttc aatcttgtga
    10261 agacccaaat ctttaaaggc taagtcaatc aaggcacgcg cagcttctgg cacataacct
    10321 cgcccccaat agtctgagtg taaggtatag ccgatttcca tcacatcatc ttcatggcga
    10381 tggttgaaat agatagagcc aacgatctta tccgttcctt tgacgacaat cccgtagcct
    10441 gctgggagat tttccttttg attacgctcc ggaagaatgt gctccagata ataaatctca
    10501 tcttccaagg tcttgactgg aggaaaacct gctggatagg cgacctctgg caaactagca
    10561 tagtcaaaga tatcctcagc atccgccaca gtacggactc gtaaaacgag acgttctgtt
    10621 tcgattttat ctggcagctc agttcttgcc atccttcttc ctcgcttttt tgatgaaact
    10681 gcccttcata tctacacgct tgtccagata gcgataaacg cgctgatatc catctcccat
    10741 gaaataggtt ggggcaaaca gttgattttt aaaatgtccc ttttcatcca ggagttctgg
    10801 ggcaacaagt cgctcaagaa tcttggcaaa gatgtggcaa ataccgtctt cctcaacaat
    10861 cctatctacc cgacaatcta aaacaagtgg acaggcgtct aaaataggag tctgagttcg
    10921 ttcagaaatt tcataatgca ctcccaaacg ttccaatttc tcctgatgac tgataaaacc
    10981 agcctgctcc atcgcaagca tagaagtttc atcagaaata ttcacagtaa atttttgata
    11041 ctgtttgatc tactctgcgg cattctctct cgcaacgact ccaatcacaa cccaatctcc
    11101 tagactataa gaggaactac aggtcgtgat gttatagcca aaattctaat cttgatatcc
    11161 taaaataaaa acaggaaaac cataatatag tttacttgtg ttaaaagatt gcttcataac
    11221 aacccccttt gactaagacg taaaagaaaa gccctgccat ctacatgaca gggacgaatg
    11281 tgtttatccg cggtaccacc caatttcggg cagttgcccg caactctcgt ctttaaagca
    11341 aaaagacact tttatttcaa tttttcatcc attagcaacc agttttgaca catttgtgga
    11401 cttctcagcg ccgccacttt ctgtaaaatg tgggattcaa aacacctcta atggctctat
    11461 tgtagcaaga ttttttcgga aatgcaaggc tcaagaaaaa ttacttgaac ttgatgccat
    11521 tttctttcaa tttcttgatg gtagctgggc cgattccttt caagtcaagg agttcttttt
    11581 cagtccagtt tttgaaatct gaagcagact tgattccttc atcatagaaa gtcttggcac
    11641 gatcaagtgg aagtccaccc aaatttgctg caaaatcttc tacagaatta gctacttttt
    11701 gagcttgctc tttggtagct tctactgctt gttcaacttt tttagaaaca gccttaccag
    11761 ctgcgcttgc tgactgttct gcacgcttca cgactttttt tgtctcatct acaaccttag
    11821 tcacagtact tgaaaaggca cctgcgcgac ggaggccatt tcgtaattgt tttttacgat
    11881 tgagtttctt tgacatgata aaatcctttc aataaaaaat ccctgctctg acaaggattt
    11941 aacataaaca ttctatcaag attttagtga aaaatcaaga atctttctcg tttaataatt
    12001 tcgctcacca aacaagcctt ctggcaaatc atggaatccc ttgcctactt tgaagttttc
    12061 aaacttacca agcaagaact gataagcatc caagcgactc tcgtagcgaa tcatggcatc
    12121 tttggcacgc tcgtcctggt cttcttcgta aactttttgg ctttccttgt gcgccatgtc
    12181 gttaatcatg atttgggtat tgacccaagc ttcaaattcc ttcataaatt cttgttcgta
    12241 actcattttt tctccttatt ctaatccacg gaaatagtcc gtatcaatct cacggtaggg
    12301 ctggatatcc tgaacgtatt ccagtacacc ttggaattct ccgttttcat cgtgtactgc
    12361 agcataggtg atgtggacaa acttgcctcg cgactcagac ttgaaccaca tttcatactt
    12421 gtccttggtc ccctcacgaa gtcccttcat gatagccttg accttgtcca aatacttagg
    12481 cggatgacag agttcgacat tgcgcccgac ttgggacggc gtccgtttga aaatcatctc
    12541 atcagctggc gtattgtcat tgtaatactg gaaaatatct tctttattga caaaggtaat
    12601 ctccataggg agatgattga ggatgagatt ggcctgctcg actgaaagat agccattacc
    12661 aaaagcctgt tgactatggc ggtccagcac agcttccttt tccttagggg taaaggtaat
    12721 ggtaaaatgg ccttctggcg tatctatgac ttgttgaact tgaccttctg ccgtatctag
    12781 ctgtacaggc tcctctgcaa tcttttcctc aataaagctc tgtcgttctg gcacccattt
    12841 ctctgacgga cggatgatgg catagccata ggcatcgctc tcctccgcaa tctgaagcca
    12901 gtcatcctga gtaaaagact caaggagaat catgaggagg atggactctt ccttgaaaat
    12961 catactttca aactctgtcg caaaagcttc aaaagcttcc tttacactgc taattgacac
    13021 ttctggtagt gacttggctg tcgttagagc tgtttgaaag agttccctaa tctgatcatc
    13081 cactccccac ataactttgg gaggtgaatc gtgtccatag cgctccatga taggaaagaa
    13141 gagttcttcc ttacgttggt aatggatgtc aaattgaccc acaagtccca tctgacgcac
    13201 caaaccctta cgcatctccg ccagcatttc ctcgtcttcc atagactcat aggtatctaa
    13261 caatctacga atgcgaatca aggccgcacg gagagccaga ttttcttctt tgaagacacg
    13321 aactgggtga cctggatgct cagtatctga aacttcgaca cctttgatag catttttaaa
    13381 aagattggca tggacatcac agagttccat aacatcttca aaggtgacac ctgagtctga
    13441 gttcatcagc tcgtgctcca taagggaaat ctcaatggct gagacacccg caaaggtcgc
    13501 atcaaaacgc tcctgaaccg actcaggaga ggcgccatta tgcaattcta ataaaatatc
    13561 ccgtaggaca tgaatccgtt catctgccat tagtctaatc caatcacttc gtagccattc
    13621 gcttccagtg tgcgtacaat cttgtccata ggagttcctg ctagcttaga accttgttta
    13681 agtgatactt tacgaccaac tgtattgcgc attaagggat tggcaagggg tttaaaaccc
    13741 aactccacta gaatttccaa gacttctgga tgcttgtcca ccacttctgc aacaggaatt
    13801 gacacatcga tgatattgtc catgacgacc tccattgtat gttctaatac tcaatgaaaa
    13861 tcaaagagca aactaggaag ctagccgcag gttgctcaaa acagtgcttt gaggttgtag
    13921 atagaactga cgaagtcagc tcaaaacacc gttttgaggt tgtggataga actgacgaag
    13981 tcagctcaaa acaccgtttt gaggttgtgg atagaactga cgaagtcagc tcaaaacacc
    14041 gttttg
//
LOCUS       DAVKFH010000044        13992 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKFH010000044 DAVKFH010000000
VERSION     DAVKFH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.044"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..463)
                     /locus_tag="VW006_001497"
     CDS             complement(<1..463)
                     /locus_tag="VW006_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380326.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCG"
     gene            complement(453..1448)
                     /locus_tag="VW006_001498"
     CDS             complement(453..1448)
                     /locus_tag="VW006_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380327.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYKYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHILKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(1856..2281)
                     /locus_tag="VW006_001499"
     CDS             complement(1856..2281)
                     /locus_tag="VW006_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380328.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2425..2793)
                     /locus_tag="VW006_001500"
     CDS             complement(2425..2793)
                     /locus_tag="VW006_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380329.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKK"
     gene            complement(2815..3021)
                     /locus_tag="VW006_001501"
     CDS             complement(2815..3021)
                     /locus_tag="VW006_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380330.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(3008..3169)
                     /locus_tag="VW006_001502"
     CDS             complement(3008..3169)
                     /locus_tag="VW006_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380331.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3528..4484)
                     /gene="lytA"
                     /locus_tag="VW006_001503"
     CDS             complement(3528..4484)
                     /gene="lytA"
                     /locus_tag="VW006_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9380332.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(4860..6230)
                     /locus_tag="VW006_001504"
     CDS             complement(4860..6230)
                     /locus_tag="VW006_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9380333.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(6537..7703)
                     /gene="recA"
                     /locus_tag="VW006_001505"
     CDS             complement(6537..7703)
                     /gene="recA"
                     /locus_tag="VW006_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9380334.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(7758..9014)
                     /locus_tag="VW006_001506"
     CDS             complement(7758..9014)
                     /locus_tag="VW006_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9380335.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(9099..10115)
                     /locus_tag="VW006_001507"
     CDS             complement(9099..10115)
                     /locus_tag="VW006_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9380336.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(10123..10641)
                     /locus_tag="VW006_001508"
     CDS             complement(10123..10641)
                     /locus_tag="VW006_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380337.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGELFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(10631..11074)
                     /gene="tsaE"
                     /locus_tag="VW006_001509"
     CDS             complement(10631..11074)
                     /gene="tsaE"
                     /locus_tag="VW006_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9380338.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(11160..11780)
                     /locus_tag="VW006_001510"
     CDS             complement(11160..11780)
                     /locus_tag="VW006_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380339.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTVFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(12163..13026)
                     /locus_tag="VW006_001511"
     CDS             complement(12163..13026)
                     /locus_tag="VW006_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380340.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            13491..13715
                     /gene="pneA1"
                     /locus_tag="VW006_001512"
     CDS             13491..13715
                     /gene="pneA1"
                     /locus_tag="VW006_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU9380341.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            13722..13910
                     /gene="pneA2"
                     /locus_tag="VW006_001513"
     CDS             13722..13910
                     /gene="pneA2"
                     /locus_tag="VW006_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU9380342.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT         4348 a         2944 c         2249 g         4451 t
ORIGIN      
        1 gaccacaatt actcaatacc gaatacccct gaatggtttc ttttacatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca aaatataagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat agttcaatca
      181 tagaaggaaa ctcagaatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa aattccttat
      301 tttttacttc atcaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcctttt
      661 aaaatatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaatccgt
      841 tttttgatgg gattaaataa acatgacacg gattttaaga ataattcttt ctttgacacc
      901 gtatatttgt aaagcttatt ttgcttgttt aaccaatata aagctttaaa agtcctcaaa
      961 cacctataaa ttgttgagtt tggaagtcca gtgacttgtg aaagcaaatc aacatctact
     1021 actttttgac cttttgtcaa taaaaaggca atccacgtta attgttcgct aggtgttaat
     1081 tccttaggga cttcagtatc attcgcattg agtactagtc ccaatggagg gaagaagagg
     1141 tttcccttaa agtctacaaa cggaactcta gcttgaagta attgcttttt ttcactgtct
     1201 gataacttcg aaaacaccaa aacaacatcc atattggctt tttcacccat agtgcgagcc
     1261 tgagtaacaa atgaactcaa actccccctt ctcttttctt taatcaataa aaatgattcc
     1321 ttatgaaaag tcatttgagt atactcaaac ctttttacaa aactgattgg taaagtcaaa
     1381 gtcagttcct caatactgac atcaacataa aaggattgaa aaattgacat tatctttttg
     1441 acattcatgt tttttcctta ttctatcatt tttattatat tttatcattc ggtataataa
     1501 aatataagtt ttttgataaa ataaccaaaa actactccct atcccctctc acattaccct
     1561 acatatcgtt tgacatgcga ctgatagttc aggaaaactt caaggagaac ttttctctca
     1621 tccactatgc aggacttact atttcacttc tactcctata ggctcaattt gacacttttc
     1681 tttttcgaat tgctcatatg cctcctgaat agtgagttcg atttatccaa atttattttt
     1741 gtctattttg atgaatactt ccacgaagat agtctgaaaa tgaatttgca ccaattttcc
     1801 ttctcttttt aagcatatta ttccagatat gattgccctc ttgttcagta ataaattacg
     1861 ctttaaacgc ttcaatcagt atatctcctg ttgtcatatg ttctaaagaa aattcttcta
     1921 catatgattt aacatctctt aggttattac ttcctaatat cccattatgc tttttcgcta
     1981 aggaaataga agccgcttct cccttaccaa taatcttgtt actatcatga tttcttgtta
     2041 aatctctata taatgcgtat tcttcagttc caatgtctat gctcacaatc tcagctgaac
     2101 ccttagctac caactgatct atcctagatt ttaaatgggg aattgtaggt atattgattt
     2161 catcatacac ctcttgtgga ataacaattt tacccgaata gagcttttct aaaagatgtt
     2221 cagtaccaac ccataaaaat actgaaatac aatctgcatc aataaataca cgactagtca
     2281 aacgacaact cccccctctt catctagccc atatacaata tcatatctga aagcatctag
     2341 taacagttcc tcatacttcc cttgcgaaat tctgttattt tctaaaagtt gttcagttga
     2401 attaacatat tatcctaata tcatttactt tttactttct gacaaaggac gatataaact
     2461 tgtatcatag cctaatcttg aagctgtctc tataacacta atatccatat ttttaatttc
     2521 ttctgcatca aggtatccat cattcctcaa tctatataac ataactttat gactgatacc
     2581 ataaaactga cccaatttta taatatcttc tacttcaaga tgagttctat tggcattttc
     2641 tctaatttcc tcaaccatcc tatacagtga agatgggaaa attaaaaaat aagaagcaaa
     2701 ctgatccgct tttctttcag tttcatctcc ttcaccaatc aagataagac tgactgaact
     2761 cttcttcacc tcatcataat aaagatgata cagttcatgt gctaaagaaa atctttacct
     2821 tcctaatgac atgtctgaat tgactgcaat gagactgaac tgagttcctt tataacagac
     2881 tccgctaata ttctttccga gtccataaaa taccagcgtc aaattttcta tcttttgtac
     2941 caatttaaaa atatctatcg gcgattcacc atcagctccc aacttttttc taagatttga
     3001 agctttatta cttaaatcca ttcgtgatat tcctttcacc tttttccatt ttggcaatca
     3061 tgctttgatt caaagacaaa tactcagcaa tcttatcttg agtaagattt gaagatattc
     3121 taagttcttt tattcttttt ccaacatcac atacatttat cacatacatt tatcatattc
     3181 atacacctct cataaaaaga atagcacact tttgtcataa ttttttaaat aaaaaaatta
     3241 tgacaaaaca aggaagcaat ttattgatgc tgcttaaaaa tctaaaattg atgagttcct
     3301 atcaaaaatc gtatcttcaa cctcaaaaca gtacttaaag ctatccgact cggtttacat
     3361 tgtcaaattt agattttatt tgagcataac tttctagttt gctttctgat ttttgtttaa
     3421 tatagtagca aacattgaag gaaatatctc cacaaaaaaa cgcatactat taagcttttt
     3481 caagacctaa taatatgcgc tgttctgatt tgaaagacat tccattatta ttttactgta
     3541 atcaagccat ctggctctac tgtgaattct ggcttgtctg ccagtgttcc gtctggtttg
     3601 aggtagtacc agcctgttcc gtccgctgac tggataaagg catttgatac catggcgcct
     3661 tctttagcgt ctaagtagta ccaagtgtcc ttgtacttga cccagcctgt cttcatggca
     3721 ccttcttcgt tgaaatagta ccacttatca gcgattttct tccagcctgt agccatttcg
     3781 cctgagttgt cgaaccagta ccagttgccg tctgtgtgct tcctccagcg gtctgcaagc
     3841 atatagcctg aactgtcaaa gtagtaccaa gtgccattga ttttctcaaa cttgtctttt
     3901 ggataagagc cgtctgaatg tacgtaccag tagccagtgt cattcttctg ccagcctgtt
     3961 tcaatcgtca agccgttctc aatatcatac ttaaactgct cacggctaat gccccatttt
     4021 gccaagtaag ggtatggatc cacatggtct gagtggttgt ttggttggtt attcgtgcaa
     4081 tactcgtgcg ttttaattcc agctaaactc cctgtatcaa gcgttttcgg caaacctgct
     4141 tcatctgcta gattgcgtaa gagttcgata taaaggcggt agtccgtcat gaactcttct
     4201 ttagttgaat ggctttcaat cagttcaacc gctgcatagg tttcagcatt ccaaccgccc
     4261 ccaacgtccc aggcaccatt atcaacaggt cctacctgca tgatgcaacc gttcccaaca
     4321 atgtgcgaga aaaaacctaa ttctgggtct ttccgccagt gataatccgc ttcattctgt
     4381 acggttgaat gcggattccc agttgagtgt gcgtgtactt gcctatatgg ttgcacgccg
     4441 acttgaggca aatctgttct taatttactc acattaattt ccatattcta ctccttatca
     4501 attaaaacaa ctcatttttt acaatccaga accagaaact cctttatttc taccttacaa
     4561 agaagacaat cttagtcacg attaggcttg tagatagaac ctcaaaacgc actattttga
     4621 cactgtaaat aggactgaca aggtctgcat tctatctaca ataacacccc agactaaaaa
     4681 gcttttcaaa agtatatttt tacagtctct atatgtcctt ttcataaata ctatacttta
     4741 ttatatcata taaaagaagt caaaagtctg ttaaactatt ttcaacacca aactaaagaa
     4801 gagaacacaa gttttttcga tgttcactag aggaaatgga ttttattcag taaatccaac
     4861 taggattgca ctttggttgc caaaattgcc tttccttctt ttatcaaggg atgacggaat
     4921 agtgagaagt acagttgaac tgtcatggca acccagataa gaggttcaca aaggataaca
     4981 cccttatatc ctgcccaagg aataatcaaa accacaaaaa cgatttttcc gattagttca
     5041 ataaagctag aaactagagg aaggatcttc tgccccaagc cctgcaagca attgcgataa
     5101 atcaacaaga ggctcaaaat gggataaaag gctgaactga tttgcagata gagacttcca
     5161 ttttctatca agtaaccatc tgtcgaacta gccaagaagg aaaccaaggc tggactggca
     5221 aaaaagagga aaatacaaac aaaaactgcc caggatatac ttaaacgact gccgattcga
     5281 agaccttgaa caatgcggtc aggtcgctta gctcctagat tctgagaagc aaaggtcgtc
     5341 attgatgcag aaatagcggt cataggaaga agggcaaagg tcataatgcg tcgagctgcc
     5401 gtctgggcac taataatcac tgcaccaaat gtattaacag aaaactgtaa aatcacactg
     5461 ccgatagata caattgaact catcaagccc atagccaaac cttgctccaa gagatccgcg
     5521 tacaagcttt tgtcccattt gaaatgttta aactgtggca agagttctgg cacactttta
     5581 cgaatataat aaaagcagag aaccgctgat aaaccttgcg aaataatggt agcaagtcct
     5641 gcggattgaa ctcccagatg caattgcgta ataaaataga gatccagaac cacattaacc
     5701 aaggcagaga aaatcagaaa tcccagggct gctagactgt caccaataga ccgcaacaag
     5761 cctgcaaaaa gattataagc aaagctgaca cctacacagg tcacaatcat agaaatatat
     5821 tgataagatt gaggaagaat ttctgcagga gtatctaagt attgcaagag aggatacaag
     5881 ccaagaaagc ccagcaacat aactagaatg ctcaaaagag cacctaaaat ccaggtggct
     5941 gctactgctt ccttgatttt agtgaaattc cgagccccat aataacgagc aatgacaatc
     6001 cccatgccat tgccaacacc aagtgtaaag cctacaatca ggtcaaaaat cgctgtcgtc
     6061 gctcctactg cagccaagga ttcttgacca agaaatcgtc caacaatcaa gacatcagca
     6121 gtgttataga gctgttgaaa aatatttgat agcaagattg gaaaggtgaa gcttaagagc
     6181 gagggaagaa tcggaccatg tatcaggtcc actgttcgtt ttttattcat aaagctatta
     6241 tatcagcttt ccagaggagc gacaagaaac agcaaactta tatatagcgg aatgaaataa
     6301 ggacagtcaa atcgattttt aacaatgttt tagaagtaga tgtgtactat tctagtttcg
     6361 atctattata gttggttgag tttggaatag tacgctgtag ctgctaaaac atttctagaa
     6421 attaatttga ctttcctaat aaagttgttc atatcttatt tcaagctact atatataatc
     6481 taaaaacttg aatcaaaaat cgaaaaagta gcggatttct ccactgcttt aacagcttat
     6541 tcttcaattt cgatttcaag ttcatcgcct aagtcaagcg gaacttcttc attcactgct
     6601 tcttctttct ttggctcatc ttttttgttt tcagtatctt gttctgaaac ttcttctcca
     6661 tcaatcaagc caaatttaga acggacttgc ttatcaattt catcaaagat ttctgggtgc
     6721 tctgccaagt atttcttagc attctcagaa ccttgcccaa ttttttcatc tttgtaagaa
     6781 taccaagccc ctgctttttt gataatatcc aaatcgcttg caatcttcaa aagctcacca
     6841 gtcttagaaa ttccttctcc gtacataatt tcaactacgg cttccttaaa cggtggagct
     6901 accttatttt ttacaacctt aatcttagtt tctttaccga cattggtttc tttttgatca
     6961 ccagttccct taatttgtgt attaccacga acatccaagc ggactgaagc atagaatttc
     7021 aaagcacgtc cgcccggtgt tgtttctgga tttccaaaca tcactccaac tttttcacgc
     7081 aattggttga taaaaatggc aattgttttg gttttattga tagaggcgcc aagtttacgc
     7141 atggcctggc tcatcatacg agcctgcaaa ccaacatggc tatctccgat atctccatca
     7201 atttccgcac gaggaacaag ggcagcaact gagtcgacta cgacaagatc aactgcacct
     7261 gagtcaatca attttcccgc aatctcaaga ccttgctctc ctgagtctgg ttgagacaag
     7321 agcaattcgt caatattgac accaagggcc gcagcataag ctggatcaag ggcatgttcc
     7381 gcatcgataa aggcagcaat cccaccttct ttttgcgctt gtgcaactgc atgaagggca
     7441 accgttgtct taccagatga ctctgggcca tagatttcga tgatacgtcc cttaggataa
     7501 ccacctgagc caagggcaat gtcaagagct aaagaacctg agctcatcac ttgcaccttt
     7561 tgctccgcac gttcacccaa acgcatgatt gatcctttac caaagtcttt ctcaatcaat
     7621 ttaagagcat cattcaaggc cttttcacgt tctgccccaa atttttttga aatttcttct
     7681 aatttttttg gttttttcgc cattctattc tcctacattc taatgatcct cagacctatc
     7741 tactattata tcaaaagtta gtcacttaat aaagccttgc gaactaggtt aaaggcatgc
     7801 ataaccgcaa tatgacgtac atctgctcgg cttctgcctc caatattaac cttgataacc
     7861 tcagttcctt gctcttgcgc caagcctatg aagactgtcc caactgggtg tccttctagg
     7921 ctatctggtc ctgccactcc ggtcaaacta attccaaaat cagactgggt cttgcttcgt
     7981 gcctgctcag ccatcttctg agctgtaaat tcagacacca caccatgttc ttccaaattc
     8041 ttggcaggaa tatccaacat ccttgatttt tcctccaagc tataggtcac aaaaccaccc
     8101 ttaaatatac ttgaagctcc agaaaaattc gccacggtag cttggaaaag acctgccgtt
     8161 aaactctctg cagccgcgat ggttttccct tgccttttca gttcttctac cacaatgctg
     8221 gctaaactag tttcttcccc ataaccatag caaaagtctc gtaaagaaat tccttcgaaa
     8281 gtctggcagt ccaagatttg attttccaag atatccagcg cttgattcgc ctcttcttga
     8341 ctgctagcct ttgttgacag acgtagagtg acttctcctg tcttggcata aggggccaag
     8401 gtaggatcga tctgattatc aattaaatca gccaaaatcg taaccaactg gctctcgcca
     8461 atcccaaaga aacgaagaac tcgggaatac agcttgctcc ctgtcatcaa cttgggtaga
     8521 agttggttta agaccatggg tttcaattca cttggcggac ctggaaggac gacataggtc
     8581 actccgtcta cttctaattt tcctccaaca gccagtcctg tttcgtttgg cagtggaatc
     8641 gctccttcta caatttgagc ttgtctttcg ttattcggtg ttcgggcata gtctggtcgc
     8701 agggcaaaaa agatatccaa cttctcctga gcctgaggat caaagactaa tgctttccct
     8761 aaaaatttag ctagggtttg tttggttagg tcgtcctcag ttggccccaa accgcctgtc
     8821 aaaatcacca gactgctacg ttgactggca atctcaagca aagacaagag acgaacttca
     8881 ttgtctccta cagccgtctg aaaatataca tctaccccaa tctcagctag tttttccgac
     8941 aaaaactggg cattggtgtt gacaatctgt cctgtcaaaa tctctgttcc aacagcaatg
     9001 atttctgctt tcatgtttcc tcctacctat ctattcgtat ttttttgaaa aaatcgcagg
     9061 aattttccta cgattgattt ttttatttgt atcaaaagtt aattatcttc atcaccaaca
     9121 ggtgctctgc caaataaatc ttcaaataaa accgcattgg tttcaagctg agtaacttct
     9181 tcttgtccca aagaacgtcg gagtatattt tgcatttcca acatatgtgc tctcgaaaca
     9241 atctggtaag aaacaccttg aagtatctct ccttcaccct gcaactgctg agtttcaatg
     9301 gttttaaatg aatctttata gcctagcaag ttagggatac tttttgcaga caaatcaata
     9361 ttggtctgca tattgtcact caaagctttt agaatctctt gataatgacc aacgctattt
     9421 aaactgagag ctttttccat gactttttga ataacttcac gttgacgttt ttgacgacca
     9481 taatccccct caggatcttg gtaacgcatt cgtgcataga ctagggcttc ttctccccca
     9541 atatgttgct ccccaacacc gatagaaata gtattaaatt cttcttggtc actgatagaa
     9601 attgggaaac ctaggatatt attgactgta atacctccta ctgcatccac tagtttttgc
     9661 aaccctctca tattgaccat cacatagcga tcaatatgga tattcatcat tttttgaatg
     9721 gtttctatag caagctctgc tccaccatct gcatatgctg agttcagttt cgcttcatga
     9781 gcctgaccat tccctgattc aatgcgcgtc agaatatccc gctctaaact catcattgtt
     9841 gtttttttcg ttttaggatt cactgtcatc aagatcatgc tatcacttct accgacccaa
     9901 gtttcagttc gttcaacatt tccggtgtcc actcccatta acagaatggt tagaggttca
     9961 gtcgcttcaa taaccttggt ttcttcaccg atttttttat aggttttagc taaggtttct
    10021 gtcccttgtt gataaatagt ataagcaaaa acacctactc ctactacagt tacagaaagt
    10081 aaagctagca ccattccaat aattttttta accatatttc tactaaccta tcagtttacc
    10141 catcaagtaa acatcgataa atttcccttc ttctatatat gccccacgct cttggctacc
    10201 ttcaatgaca aagccatgct tttgataaag atggactgct gcttgattac gagtttggac
    10261 agtcagttgg agacgacgca gaatgccact tgcttgtgcc cactctatcg cttcttctag
    10321 caacaaactt cccaagccat tattccaata tctttttcca atcacaatga agagttctcc
    10381 aatatgacgg actctcttac gctggtcagc tgtaatattt acaataccag caattttgcc
    10441 atttaagaat gcaagtaagg ttatctgatt gtccgaacta gcttgcttgt tgaggaatat
    10501 ttccatctcc tcactagtca agagaatacc atctccgtct aggctggtaa agtctgtctc
    10561 caaactcaca cgatttaaaa aggccactaa ttcagctgca tctttgggct ctgcttccct
    10621 aatgagcaat tcatactcca tattgaagct cctctaacaa tttctcagca cgcaaaccct
    10681 ttgcctgaaa atttaaacgg cgtccatctg cttcttttag aatttccaat tctaaataag
    10741 catctggcaa ggcatctcct aagagattgc cccactcaat aacagtcacg ccgccaccaa
    10801 agataaactc atccaagtcg atagaatcag catctccttc aatacgataa acatctaggt
    10861 gataaagtgg aagtcgacct tcatactctc tcacgatagt ataggtggga cttttaatca
    10921 tttgagaaat ctgtaatcct tttgcaagtc ctttagtaaa ggtcgtttta cctgcaccca
    10981 gttctccagt taagattaaa acatcattct ttgctaatag atggcccaaa cgctccccta
    11041 aggcttgcaa ctcttcttca ttttttgtgt acatactctt attataccaa aaacttttct
    11101 tttgtgtcta ttttcctact aaacttatca tcataacatc cataaaaaac aggctttctc
    11161 taaaagaaaa tgagcgtaac aatgaccaat acaagatctc ggaaaatatg accataaaag
    11221 gaaacttcct tcttaaccga atttgggaca agataggctg caaaaaacaa gcccagtcca
    11281 atataaatca gaagtgagac aatggtcatt ggatttctta agaaaagaag tgttgctaaa
    11341 atagtcacca acactgtctt ttttctgtct agcatagcaa gaaaatcgcg cacgtatttt
    11401 ttcaagggta aaaaaatcag caaatctagc ccaaatagga aaaagaagga tggcaataaa
    11461 aagtcaacta attcttgctg cagcgtattt ttgatgaaca agttatctga caaaacaaga
    11521 acagctccta acaaattaat taagagtaac atactgtaaa aaagcttcac agacttctta
    11581 ctggctagga cactatggac ttcttgctta cgggtataaa gataatttac tccagcacag
    11641 attcctgaaa cgaaaaccat gcttccgatg aaaaaaactg tactttgttt aaaggacaag
    11701 atgcattcct tccataggaa acagctactc aaactgattt gaattaaagc taacaaaaat
    11761 aagattctca ttgatttcat cttctctctc ccttcctacc aatcattata ctaggagaaa
    11821 agagagaact gtttctaatc ttctcaaatg tctctttaag acgctaaaca aacactagag
    11881 actaatactc aatgaaaatc aaagatcaaa ctaggtagct agccacaggt tgctcaaaac
    11941 actgttttga gattgcagat agagctgacg tgatttgaag agattttcga agaatataaa
    12001 tttgaaatca tgaaaatccg tcaaacgggt ggttgttttg tctcgcacct cacggagcga
    12061 gacggactca gagtcacata attataaggc tgatagtatt aatctaacta tcagccttac
    12121 aggttattta acgtttcaga aaaactataa tgtcaagatt aactaaacag tatctaattc
    12181 cttcaaataa ttttctatct tcatcaacat taaaggattg ttataaatct tacataactc
    12241 tcttgcttct atataataat ttttgacttg ttctctgtct agaaatttgg ctccagcatt
    12301 tcctacaaga ataagtagag gagccaattg gtagcttgtc tgtctttgtt tacagagttc
    12361 aatcgtttca agagcttctt ggatggcttc attatatttt tcctttgata ctaggtagtg
    12421 agcgtagttg taacgaactc tgatgtagcc aaataaaaac tcttgatggt ccaaattttt
    12481 tgtctgatac aactctatta aatgagagta gtttgcctca tattcttgtt cacgacccac
    12541 taaggaatag aaattagata gagtattcaa cgcctttaaa taaatcagag tatttgaaga
    12601 gacttttaat aatatatttt ccaatgacga aattgcctca cacttactgt catattgata
    12661 gaagtcaatt atagatttaa tccattcaag gtaagttcgg tcttctaatg ttagaaaagt
    12721 gcttcgttct acctctattt tataaagata ttctaaatcg tcataatttc tgtcatctaa
    12781 taggcgagca gatagatgtt tgaaattaga gaggttagac ttaacttcga tttgttcatt
    12841 gaaaaagtaa tccaaaggga cttcaagtcg ttgagagagt ttgaataaca agtctgcgga
    12901 gggaataaaa tgacctcttt caattttact aatctggctt tgttcacaaa ttccttctgc
    12961 aagagtttgt tgggagagtc tcaactcttt tcttttcaat ctgaatttct ctgcgagtgt
    13021 attcattaaa gataataaac cttcctattt gcttaatttc attataaagt ttttagttca
    13081 aaatagcaag ttaaaggaat aaataattct ttatggtaat aaacaaaaaa aaaagttaaa
    13141 aattacataa aaatacttga ctaaataaaa tatatctgtt agaataaaaa caaggaaaaa
    13201 gaaaggggtt tcattgcatg agaaaaaaac gtggaattaa aaaattagtt acatttgcat
    13261 tgctaggtgt ttttatgttt agtaatacaa ttccttacca acagtttatt cagaagaata
    13321 aacaattgga gattcgagtg taatcgcaaa agaagtccaa tggtcttgat gttgggaagg
    13381 ctgattaaaa cccgatattt tgcacgagaa tgagagaatt ataatttatc tgaaaaaacg
    13441 aaaaatatag agcaacttat ttaacttttt ataaggagga gttattactt atgaccaact
    13501 ttaattcaaa cgaaaaattt tgtggaaaaa gtttaaaatc attgtctgct gatgaaatga
    13561 gtttaattta tggtgcatct gatggtgcag agccacgttg gacgcctact cctattattt
    13621 tgaaatcagc tgcagctagc tcgaaagtat gcattagtgc agctgtttct ggtattgggg
    13681 ggctagttag ctacaataat gattgtttgg ggtaaattaa aatgaaaaat gattttgtaa
    13741 tcggaaagag tttgaaagaa ttatctcttg aagaaatgca attggtttat ggtggcacag
    13801 atggtgctga ccctagatca acaattatat gttctgctac attaagtttt attgcatcat
    13861 atttggggtc agctcagact cgttgtggca aagacaacaa gaaaaaataa aatattcaat
    13921 tttaaaaata aaagttgctc tttattttcc tctctcgtaa ctaataattt tgagagaggt
    13981 ttttttttaa aa
//
LOCUS       DAVKFH010000045        13936 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKFH010000045 DAVKFH010000000
VERSION     DAVKFH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.045"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(56..253)
                     /locus_tag="VW006_001514"
     CDS             complement(56..253)
                     /locus_tag="VW006_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9380343.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(328..1104)
                     /locus_tag="VW006_001515"
     CDS             complement(328..1104)
                     /locus_tag="VW006_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9380344.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1226..1972
                     /locus_tag="VW006_001516"
     CDS             1226..1972
                     /locus_tag="VW006_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9380345.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1985..2965
                     /locus_tag="VW006_001517"
     CDS             1985..2965
                     /locus_tag="VW006_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9380346.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3160..3480
                     /locus_tag="VW006_001518"
     CDS             3160..3480
                     /locus_tag="VW006_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9380347.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3526..3834
                     /locus_tag="VW006_001519"
     CDS             3526..3834
                     /locus_tag="VW006_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9380348.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3851..5149
                     /locus_tag="VW006_001520"
     CDS             3851..5149
                     /locus_tag="VW006_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9380349.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5300..7738
                     /locus_tag="VW006_001521"
     CDS             5300..7738
                     /locus_tag="VW006_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9380350.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7757..8425
                     /locus_tag="VW006_001522"
     CDS             7757..8425
                     /locus_tag="VW006_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9380351.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            8443..9531
                     /locus_tag="VW006_001523"
     CDS             8443..9531
                     /locus_tag="VW006_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9380352.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            9986..12487
                     /locus_tag="VW006_001524"
     CDS             9986..12487
                     /locus_tag="VW006_001524"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9380353.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            12681..13376
                     /locus_tag="VW006_001525"
     CDS             12681..13376
                     /locus_tag="VW006_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380354.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            13388..13804
                     /locus_tag="VW006_001526"
     CDS             13388..13804
                     /locus_tag="VW006_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380355.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
BASE COUNT         4481 a         2166 c         2920 g         4369 t
ORIGIN      
        1 atcttatttc aattcactat attagagtaa aattctctac aaaaagaaga atagcctatt
       61 tgactattct tccgagtgat ttcaatttct ttggggaaat atggagatac tttttaaata
      121 ctttggcaaa atatttatag tctgaaaatc caacttggtc agagatagcg cttaaagggg
      181 agttgggaga ttcagccatc ttatctatag cttgtttcag gcgatattgt aagatatatt
      241 catttagagt catggggaag tcttctttga taaccttata tagatgcttg ttatctgcaa
      301 atagctagag catttcaagg aaataaacta gaaataacaa ttaatatggt ggttcagaaa
      361 taccttttga taatcaataa gatcttcagg atgaagtgcg ttgattccat ccatttcata
      421 tttccgattt aataaacgat atttgttttc accaaattga tgaaaaggga gtagttgaac
      481 ttggtcgata tttaatgagt taaatagagt agcgaatttt tctgcatcct ctaaactatt
      541 gttaaaatta ggaataactg ggattcttaa aacgatagtt ttattttgag aaaaagcata
      601 atgaatgttt ttaataatca tttgattaaa aaccccagtc acttttttat gttttataga
      661 attataatgt tttaggtctg tgtagataaa atccacatat tgaattaaat caataaattt
      721 ttcatgatca acaaaggcag tagtttcaat ggcagtgtgt atgtgatgtt ctttagctga
      781 ttttaagatg gctttagcaa attcaaactg agcaaatatt tcacctcctg ataaagttaa
      841 acctccaccg gattcttcgt aaaattcttt gtcttttaat acctctgtaa taatttcttc
      901 tacagtcttt tcttcaccga ctaaggtgaa tttctctcgt tgagcatctt tcattttttc
      961 aggtttcatt ctttgagatt caggattaga acaccatgga cagcgcagag gacatccttt
     1021 taaaaaaaca gttgtacgaa tacccgggcc gtcatgaatt gaaaagtgtt gaatattaaa
     1081 aataattcct ttagatattt ccataagagt tcctcctctt cataagtcta tcctatcaca
     1141 aaaacgaaag aaaaacaatt acgaacgaaa gtttaatttg ttgttttttg ctttttattt
     1201 tggtagaata aaacggaggt gtaaaatgaa aagattggaa caaattatta aattagtatc
     1261 agaatatgaa aagatcgacg ttaatacatt atcggaaaaa ttaaatgtat cgaaagtaac
     1321 gattagaaaa gatttagata aattagagtc aaaaggttta ttacacagag agcatggata
     1381 tgctgtatta aatagtggag atgacttaaa tgtacgtttg tcaattaact atgaaattaa
     1441 gagaaaaatt gttcaggaag cagtaaaatt ggtgtcagat aatgaaacaa taatgataga
     1501 atctggatcg acctgtgctt tacttgctga ggaaatttgc aagcaaaaaa gaaatgttac
     1561 gattgtaaca aattcatttt ttatagcaaa ttttgtgaga gcttatgatt catgtcgtat
     1621 tattgttctt ggtggagagt ttcagaagga ttcacaggtt actgtgggac ctttattaaa
     1681 agaaatgata cagacttttc atgtgcatca agcttttgtt gggacagatg gttacgataa
     1741 agagatgggc tttacaggaa aagatttaat gcgcagtgag gtagttcaat atatttcagc
     1801 agcgtcggat aaagtcattg tactaactga ctcaagtaaa tttgataaaa ggggtacagt
     1861 aagaagattt gctttaagtc aagtctatga agtaataaca gacgaaaaac tttctaaaca
     1921 aaatatagct acattagaaa atgctgggat aatggttaag gtagtttcgt aagaggttaa
     1981 atgtatgaat caagatagga ataaactgct ctctaaaatt gcttatctgt attatattga
     2041 aaacttaaat cagtcacaaa tagcagcaaa attaggaatt tatagaacct ctattagtag
     2101 aatgttaaca gaagcaagga atgcaggaat tgttaaaatt gaaatagaga attttgatac
     2161 caatatgttt aagttggaaa attatgtaaa agaaaaatat ggtctggaaa gtttagaaat
     2221 tattccaaat gaatttgatg ataatccaac aattttatct gaaagaattt ctcaagttgc
     2281 agcaggtgtc cttaggaatc taattgatga taatatgaaa attggctttt cttgggggaa
     2341 aagtttaagt aatttagtag atttaattca cagtaaaagt gtccgaaatg ttcacttcta
     2401 tcctctagca ggtggtccta gtcacataca cgctaaatac catgtgaata cactgattta
     2461 tgaaatgtct agaaaatttc atggagagtg tacatttatg aatgcaacga ttgtgcaaga
     2521 aaataaattg ttagcagatg gtattttgca atcaagatat tttgaaaatt tgaaaaatag
     2581 ttggaaagat ttagatatag ctgtagtcgg aattggtgat tttagcaata aaggaaagca
     2641 tcaatggtta gacatgctta cagaggatga ctttaaagaa ttgaccaaag ttaaaactgt
     2701 aggagaaatt tgttgtcgat tttttgattc aaaaggtaaa gaagtctatg aaaacttaca
     2761 ggaaagaacg atagcaatct ctttggaaga tttaaaaaat attcctcaaa gtttagctgt
     2821 tgcttacggt gatacgaaag tatcttcgat tctttctgtc ttgcgtgcta atttagtaaa
     2881 tcatttgatt acagacaaaa atacaatttt aaaagttttg gaagaagatg gggatttgac
     2941 ttttagagag attctaggtg agtgaaaatg atagactgat cagtttatcg ttttttcttt
     3001 ttagtcgatt gcacatttgt gctcatataa acaaaaatag tttatctgtt gtttttggat
     3061 tgacaacttt attatgtagt tgtattctat agttacaaaa gaaaatttta aaatttcaaa
     3121 tgaaaaaagc tttttacata gtgaaatgag gaggaattta tggaaattat tgttccagat
     3181 caaattatca tgggtttaat tttatatgct ggtgatgcga aacaacatat ttataaagcg
     3241 ttagattaca taaaaaatgg tacatgtgaa cggtgtgaag aagaaatcca gttagctgat
     3301 gccgccttat tagaagccca taatctacaa acaaaattct tggcacaaga agcgtctggt
     3361 acaaagacag aaattacagc tctctttgtt cattcacaag atcatctcat gactagtatg
     3421 acggagatta atttaatcaa agaaattatt agtttgagaa aagaacttca taaaaaataa
     3481 tactagagta ttatcattgt tattaacata gaggaggaaa acataatggt gaagattggt
     3541 ttgttttgtg cagcaggttt ttctactggt atgcttgtaa ataatatgaa aattgcagcg
     3601 caagctagtg gagttgaggc agaaatagag gcgttttctc agtctaaatt agcggattat
     3661 gcgccaaata tagatgttgc actattgggt ccacaagttg cttatacatt agataaatca
     3721 aaagaaattt gtgataagtg tgatgttccg atagctgtta ttccgatgat ggactatggt
     3781 atgttagatg gaaaaaaagt attagatttg gccctatctt tgattagtgg gtaagaaaag
     3841 gagatttatt atggcaaaga tggatgttca gaaaatcatt gcaccgatga tgaagtttgt
     3901 gaatatgcgt ggcattatag ctctaaaaga tgggatgtta gcaattttgc cattgacagt
     3961 agttggtagt ttattcttga ttatgggaca attgccgttc gaaggattaa ataagagcat
     4021 tgctagtgtt tttggagcta attggacaga gccgtttatg caagtatatt caggaacttt
     4081 tgctattatg ggtctaattt cttgtttttc aattgcctat tcttatgcta agaatagcgg
     4141 agtagaggct ttaccagctg gagttctatc tgtatctgca ttctttattt tgctaagatc
     4201 atcttatatc cctaaacaag gtgaggcaat tggggatgct attagtaaag tttggtttgg
     4261 aggtcaagga attatcggtg ctatcattat aggtttggta gtaggaagta tttatacctt
     4321 ctttataaag agaaaaattg ttattaagat gccagaacaa gttccacaag ctattgccaa
     4381 acagtttgaa gcaatgattc cagcatttgt aattttctta ctttctatga ttgtatatat
     4441 tttagcgaag tcattgacta atggcggaac attcatagaa atgatttatt ctgctattca
     4501 agttccattg caaggtttaa ctggatcttg gtatggtgca attggaattg cattctttat
     4561 atcatttttg tggtggtttg gtgttcatgg acaatcggta gtaaatggag tagtgacagc
     4621 tctgctttta tctaatcttg atgctaataa agctatgtta gcctctggta atctatcatt
     4681 agaaaatggt gcacatattg ttactcaaca atttttagat tcatttttaa ttctatcagg
     4741 ttcagggatt acgtttggtc ttgtagttgc catgcttttt gcagcaaaat caaaacaata
     4801 ccaagcctta ggaaaagttg ccgcttttcc agcaatattt aacgtaaatg agccagttgt
     4861 atttggattt ccgattgtca tgaatccagt tatgtttgta cctttcattc ttgttcctgt
     4921 acttgcagct gtgatagtat atggagctat tgcaacaggt ttcatgcagc cattctcagg
     4981 ggtaacattg ccttggagta caccagctat tttatcagga tttttggtgg gtggatggca
     5041 aggagttatt actcagctgg tgatattagc gatgtctaca ttggtttatt ttccattctt
     5101 taaagtacag gatcgtttag cttaccaaaa tgaaatcaaa caatcttaga ggtatttgtg
     5161 tgttactgtt aaactcacac atttgtgcta aaaattagag agttaaaatt tttctagtta
     5221 aaagcttgaa aatttctata aaaatcggta ttatattttc gaaagaaata aaaatatttt
     5281 cgaaagaaag gtggttacga tggtaaatac agaagtagca agaacaacaa tcaagacaga
     5341 atattttggc agccttactg aaaggatgaa caaatatcga gaagatgttt taaataaaaa
     5401 accttatatt gatgctgaga gagcagttct agcaacacgc gcctatgaac gatacaagga
     5461 acaacctaat gtcctaaaac gtgcatatat gctgaaagaa attttggaaa atatgactat
     5521 ctatattgaa gaagaatcta tgattggggg aaatcaagct tcttccaata aagatgctcc
     5581 tatttttccg gaatatacgc tagaatttgt tctcaatgag ttggatcttt ttgaaaagcg
     5641 tgatggagat gttttctata ttacagaaga aacaaaagaa caacttagaa gtattgctcc
     5701 gttttgggaa aataataatt tacgtgctag agctggtgcc ttattacctg aagaagtgtc
     5761 tgtttatatg gaaacaggat tcttcggtat ggaaggtaag atgaattctg gagatgctca
     5821 cctagcagtt aactatcaga aacttttgca atttggctta agaggttttg aagagcgggc
     5881 tcgtaaagca aaagcagctc tagatttaac agatccagca agtattgata aatatcattt
     5941 ttacgactct atatttatcg taatcgatgc tattaaagta tatgcaaagc gctttgttgc
     6001 tcttgctaaa agtttagccg aaaatgcaaa tcctaaacgt aagaaagaat tacttgagat
     6061 tgcagatatt tgctctagag tcccatatga accggcaact acttttgcag aagctattca
     6121 atcagtttgg tttattcaat gtattttaca aattgaatct aatggccact ctctttcata
     6181 tggccgtttt gatcaatata tgtatccata tatgaaggct gatttagaaa gtggtaaaga
     6241 aacagaagat agcattgttg aacgtctgac aaatctttgg attaagacaa ttacaattaa
     6301 taaggttcgc agtcaatcac atacattttc ttcagcagga agtcctttat atcaaaatgt
     6361 tacaattggt ggacagactc gagataagaa ggatgctgtt aacccattat cttatttggt
     6421 attaaaatca gttgcacaaa cccatctacc gcaacctaat ctaactgtac gttaccatgc
     6481 aggtttagat gctcgtttca tgaatgagtg tattgaagtg atgaaacttg gttttggtat
     6541 gcctgcattt aataatgatg agattattat tccttctttt attgcaaaag gagtattgga
     6601 agatgatgct tatgattaca gtgccattgg atgtgttgaa acggcagttc cagggaaatg
     6661 gggctatcgt tgcacaggta tgagttatat gaacttccct aaggttctac ttatcacgat
     6721 gaatgatgga attgatccgg cttcgggtaa acggtttgca ccaagctttg gtcattttaa
     6781 ggatatgaag aacttttctg aattagaaaa tgcttgggat aaaacactaa gatatttgac
     6841 acgaatgagt gttattgttg aaaattctat tgatttatca ttggaacgag aagttcctga
     6901 tattctatgt tcagcattga ctgatgattg tattggtcgt ggaaaacacc ttaaagaagg
     6961 tggagcagta tatgattata tatcaggatt gcaagttgga attgcaaatt tgtcggattc
     7021 attagctgca attaaaaaat tggtgtttga ggaagaacgt ataagcccaa gtcagctttg
     7081 gcatgcattg gaaacagatt atgccggaga agaaggtaag gtcattcaag aaatgttgat
     7141 tcatgatgca cctaagtatg gtaatgatga tgattatgct gacaaattgg ttactgctgc
     7201 ttatgacatt tatgttgatg aaattgctaa atatcctaat acacgttatg gaagagggcc
     7261 tattggagga attcgttatt caggaacatc ttctatatca gccaacgtag ggcagggacg
     7321 tggaacatta gcaactccag atggacgcaa cgcgggtaca ccgttagcag agggttgttc
     7381 accatcacat aatatggatc aacacggccc tacatctgtt ttaaaatctg tttcaaaatt
     7441 accaacagat gaaatcgtag gtggggttct cttaaatcag aaagtaaatc ctcaaacgtt
     7501 agccaaagaa gaagataaat taaaactaat tgctttgtta cgaacattct ttaatcgttt
     7561 acatgggtac catattcaat acaatgttgt ttccagagag acgttgattg acgctcagaa
     7621 acatcctgaa aaacacagag acttaattgt tcgtgttgca ggatactctg cattcttcaa
     7681 tgttctttct aaggcaaccc aagatgacat tataggacgt actgagcata ctttgtaaaa
     7741 taaagaggtt ctttttatgg aatttatgct tgacacatta aatttagatg agattaaaaa
     7801 gtggtctgaa attttgccgc tagctggggt aacttcaaat cccactattg caaaaagaga
     7861 gggttctatt aatttttttg aacgaatcaa agatgtaaga gaattgattg gctccacacc
     7921 ctctattcat gttcaggtga tttctcaaga ttttgaaggc atcttaaagg atgctcatga
     7981 aattcgaaga caagcaggag atgatatatt tatcaaagta cctgttactc cagctggatt
     8041 acgtgcaata aaggtgctaa aaaaagaggg ctaccatatc actgcaacag ctatttatac
     8101 agttattcag ggattattag ctatcgaagc aggagcggat tacctagctc catattataa
     8161 tagaatggaa aatctgaaca ttgattcaaa ttctgtcatt cgtcaattag ttcttgctat
     8221 tgatagacag aactctccta gtaagatttt ggctgcatcc tttaaaaatg tagcacaagt
     8281 aaataatgct ttagctgcag gtgcgcatgc tgttacagca ggagcagatg tttttgaatc
     8341 agctttcgcc atgccatcta tccaaaaggc ggttgatgat ttttctgacg attggtttgt
     8401 tactcaaaat agtcgttcca tttagataga gaggaaatac atatgagaat ttttgctagt
     8461 ccttctagat atattcaggg ggaaaatgcc ttgtttgaaa atgccaaatc aattttggat
     8521 ttgggaaatt accctattct attatgcgat cagttggttt atgatattgt tggaaaacga
     8581 tttgaagatt atctacatag gtatggtttc catattgttc tggcgctatt taatggtgaa
     8641 gcttctgaca atgaaatcaa tcgagttgtt gccttggctg agaaagaaaa ttgtgatagt
     8701 attatcggtc ttggtggggg aaagacgatt gatagcgcaa aagctattgc agatttgatt
     8761 gaaaagcctg ttattattgc tccaacaatt gcatcgaccg acgcacctgt atctgcttta
     8821 tctgttattt atacagatga aggtgcattt gatcattatc tattttattc taaaaatcca
     8881 gatttagttt tggttgatac aaaagttatt tcacaagccc ctaagcgttt attagcgtct
     8941 ggtattgcag atggtttagc aacttgggtt gaggcgcgtg cggttatgca ggcaaatgga
     9001 aaaactatgt tgggacaaca gcaaacattg gctggagttg caattgcgaa gaaatgtgaa
     9061 gaaacgctgt ttgcagatgg tttacaggct atggcagctt gtgaagctaa agtggtgaca
     9121 ccagcattag aaaatattgt tgaagctaat actttattga gtggtctagg ttttgaaagt
     9181 ggaggattag ctgcggcgca tgcaattcat aatggtttta ctgcattgac aggtgacatt
     9241 catcatttaa cacatggtga aaaagtagct tatggaactt tagtacaact attattggaa
     9301 aatagaccta aagaagaact tgataagtat attgagtttt acaaaaaaat tggtatgcca
     9361 acaactctaa aagaaatgca tttggatcaa gttggatatg atgatttaat aaaagttggt
     9421 aaacaagcaa ctatggaggg tgagacaatt catcagatgc cgtttaagat ttcgccttca
     9481 gatgttgctc aagctattat cgctgtagat gcctatgtaa attcaaaata aacaataagg
     9541 actactgttt tccaaatggt agtcttttat tgatccccgt attgaattct atagaagatt
     9601 gaaataggat gagaacaaat cgattgggaa agtaaaatta atttctataa atgttttagc
     9661 aattgtttcg tactatttca gattcagtct actatacgtt cttcataaat caaaaagcga
     9721 cataggttgt cggctattta ttgtgaatac attaattagc attccagttt tatcttcggt
     9781 ctaaaataag tattttgtgc tatacgagat aagcgtcttg acttactcct tgatttactg
     9841 cataacaatg ggataaaaag tgggagatag agcaattcat agtcatcaaa attaatgaga
     9901 tacagtatac agtttttcct ttaaacacat ttcaaattcc ctcaaaaatg gtataatagt
     9961 aacatcacaa aattggagag agaccatgag tttttacaat cataaagaaa ttgagcctaa
    10021 gtggcagggc tactgggcag aacatcatac atttaagaca ggaacagatg catcaaaacc
    10081 taagttttat gcgcttgata tgttccctta tccgtctgga gctggtctgc acgtaggaca
    10141 cccagaaggt tatactgcaa ccgatatcct cagtcgttac aaacgtgcgc aaggctacaa
    10201 tgtccttcac ccaatgggtt gggatgcttt tggtttgcct gcagagcaat acgctatgga
    10261 tactggtaat gacccagcag aatttacagc ggaaaacatt gccaacttca aacgtcaaat
    10321 caatgcgctt ggattttctt atgactggga tcgtgaagtc aatacaacag atccaaacta
    10381 ctacaagtgg actcaatgga ttttcaccaa gctttacgaa aaaggcttgg cctatgaagc
    10441 tgaagtgcca gtaaactggg ttgaggaatt gggaactgcc attgccaatg aagaagtgct
    10501 tcctgacgga acttctgagc gtggaggcta tccagttgtc cgcaaaccaa tgcgccaatg
    10561 gatgctcaaa atcacggctt acgcagagcg cttgctcaat gacttagatg aactagattg
    10621 gtcagagtct atcaaggata tgcaacgcaa ctggattggt aaatcaactg gtgccaatgt
    10681 aactttcaaa gtaaaaggaa cagacaagga atttacagtc tttactactc gtccggacac
    10741 acttttcggt gcgactttca ctgtcttggc tcctgaacat gaattagtag acgctatcac
    10801 aagttcagag caagcagaag ctgtagcaga ctataaacac caagccagcc ttaagtctga
    10861 cttggttcgt acagaccttg ctaaggaaaa aactggtgtt tggacaggtg cctatgccat
    10921 caaccctgtc aatggtaagg aaatgccaat ctggattgcg gactatgttc ttgctagtta
    10981 tggaacaggt gcggttatgg ctgtgcctgc ccacgaccaa cgtgactggg aatttgccaa
    11041 acaatttgac cttccaatcg tcgaagtact tgaaggtgga aatgtcgaag aagctgccta
    11101 cacagaggat ggcctgcatg tcaattcaga cttcctagat ggattgaaca aagaagacgc
    11161 tattgccaag attgtggctt gcttggaaga aaaaggctgt ggtcaggaga aggttaccta
    11221 ccgcctccgc gactggctct ttagccgtca acgttactgg ggtgagccaa ttccaatcat
    11281 tcattgggaa gatggaactt caacagctgt tcctgaaact gaattgccgc ttgtcttgcc
    11341 tgtaaccaag gatatccgtc cttcaggtac tggtgaaagt ccactagcta acttgacaga
    11401 ttggcttgaa gtgactcgtg aagatggtgt caaaggtcgt cgtgaaacta acaccatgcc
    11461 acaatgggct ggttcaagct ggtactacct ccgctatatt gacccgcaca atactgagaa
    11521 attggctgat gaggacctcc tcaaacaatg gttgccagta gatatctacg tgggtggtgc
    11581 ggaacatgct gtacttcact tgctttatgc tcgtttctgg cataaattcc tctatgacct
    11641 cggtgttgtt ccgactaagg aaccattcca aaaactcttt aaccaaggga tgattttggg
    11701 aacaagctac cgtgaccacc gtggtgctct tgtggcaacc gacaaggttg aaaaacgtga
    11761 tggttccttc tttcatatag aaacagggga agagttggag caagcgccag ccaagatgtc
    11821 taaatcgctc aagaacgttg ttaacccaga tgatgtggtg gaacaatacg gtgccgatac
    11881 ccttcgtgtt tatgaaatgt ttatgggacc actcgatgct tcgattgctt ggtcagaaga
    11941 aggtttggaa ggaagccgta agttccttga ccgagtttac cgtttgatta caagtaaaga
    12001 aatccttgcg gaaaacaatg gtgctcttga caaggcttac aacgaaacag tcaaagctgt
    12061 tactgagcaa attgagtctc tcaaattcaa cacagctatt gcccaactta tggtctttgt
    12121 caatgctgct aacaaggaag ataagcttta tgttgactat gccaaaggct ttattcaatt
    12181 gattgcacca tttgcacctc acttggcaga agaactctgg caaacagtcg cagaaacagg
    12241 tgagtcaatc tcttatgtag cttggccaac ttgggacgaa agtaaactgg ttgaagacga
    12301 aatcgaaatc gttgtccaaa tcaaaggtaa agtccgtgcc aaactcatgg tcgctaaaga
    12361 cctatcacgt gaagaattgc aagaaattgc tctagcggat gaaaaagtca aagcagaaat
    12421 tgacggtaag gaaatcgtga aagtgattag tgtaccaaat aaattggtta atattgttgt
    12481 aaaataacca atttatttgc tgatttccaa cctcaaacag tttcccaaac tgtttgagcc
    12541 aactaaactc gttaatatcg ttgtgaaata aggtaggaat ccttcagagt agaatctgga
    12601 ggattttttg aatcttctta tgaaagtatg gtatactatg ggcaactata aagtttgaaa
    12661 agtgaaaaaa ggagaaagct atgccagtaa atgaatatgg tcaaatgatt ggggagtcaa
    12721 tggaaggtta tacaccaggt gaactgcctt ctattgattt cttagaaggg cgttatgcta
    12781 ggatagaggc tctttcagtg gaaaagcatg cggaggattt gctagctgtt tatggcccgg
    12841 atactcctcg ggagatgtgg acctaccttt ttcaggagcc agtagcagat atgggggagc
    12901 tggttagcct tttacatcag atgttggctc gtaaggaccg tttttactat gcaatcatag
    12961 acaaggcgac tggtaaggct ttgggatctt tttctctcat gcgcattgac cagaataacc
    13021 gagtaataga agtcggagca gtcacttttt ctccagagct aaggggtaca cggataggaa
    13081 ctgaagctca gtatctccta gctcgctatg tttttgagga gctaaactat cgtcgctacg
    13141 agtggaaatg cgatgcctta aatctgccat ccagacgagc tgcggagcgt ttgagcttta
    13201 tttatgaagg aaccttccgt caggcagtgg tttataaggg acgtacgaga gatacggatt
    13261 ggttgtctat gattgataag gactggcctc aagtcaaaga tcgtttggaa acatggttgc
    13321 gtcctgaaaa ttttgataaa aatggacagc agtacaagag cttgagagaa ctttaagagg
    13381 tgttgagatg attactatta aaaagcaaga aattgtcaag ctagaggatg ttttacatct
    13441 ctatcaggct gtcggttgga caaattatac ccatcaacca gagatgctgg agcaggcctt
    13501 atctcattca ttagtaattt atctggcact tgatggtgat gctgtggtgg gcttgattcg
    13561 tttggttgga gatggattct catcagtatt ggttcaggat ttaatcgttt taccaatcta
    13621 tcagcgtcaa ggaattggta gtgccttgat gaaagaggct ttagaggatt acaaagatgc
    13681 ctatcaagtc cagctggtga cagaagagac agaaagaacc ttgggcttct atcgttctat
    13741 gggctttgaa atcttatcca cctataattg tatagggatg acttggatga atcgtaaaaa
    13801 ataacaaaac ttgttttttc ttaagcaaaa tttaaggatg gtctagtatt atatagtcat
    13861 taaataaaga cctcctaact ttatttaata aaatcctaaa cttttctttt tcataataat
    13921 ctcccttaac ttcacc
//
LOCUS       DAVKFH010000046        13285 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKFH010000046 DAVKFH010000000
VERSION     DAVKFH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.046"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..193
                     /locus_tag="VW006_001527"
     CDS             <1..193
                     /locus_tag="VW006_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380356.1"
                     /translation="TNEELELIQGGADSYGKNPNGRYDWEIEPVLTLPVHGFCSRGTY
                     DLGYIGGGNHLCKGSAARF"
     gene            247..918
                     /locus_tag="VW006_001528"
     CDS             247..918
                     /locus_tag="VW006_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9380357.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(970..1167)
                     /locus_tag="VW006_001529"
     CDS             complement(970..1167)
                     /locus_tag="VW006_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380358.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1160..2068
                     /locus_tag="VW006_001530"
     CDS             1160..2068
                     /locus_tag="VW006_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9380359.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2065..2526
                     /locus_tag="VW006_001531"
     CDS             2065..2526
                     /locus_tag="VW006_001531"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9380360.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2516..3403
                     /locus_tag="VW006_001532"
     CDS             2516..3403
                     /locus_tag="VW006_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9380361.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3406..5289
                     /gene="cbpE"
                     /locus_tag="VW006_001533"
                     /gene_synonym="pce"
     CDS             3406..5289
                     /gene="cbpE"
                     /locus_tag="VW006_001533"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9380362.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5369..6499
                     /locus_tag="VW006_001534"
     CDS             5369..6499
                     /locus_tag="VW006_001534"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9380363.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6509..7771
                     /locus_tag="VW006_001535"
     CDS             6509..7771
                     /locus_tag="VW006_001535"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9380364.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7775..8572
                     /gene="proC"
                     /locus_tag="VW006_001536"
     CDS             7775..8572
                     /gene="proC"
                     /locus_tag="VW006_001536"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9380365.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            8688..9326
                     /locus_tag="VW006_001537"
     CDS             8688..9326
                     /locus_tag="VW006_001537"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9380366.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK"
     gene            9323..10213
                     /locus_tag="VW006_001538"
     CDS             9323..10213
                     /locus_tag="VW006_001538"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9380367.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10253..10570
                     /gene="yabA"
                     /locus_tag="VW006_001539"
     CDS             10253..10570
                     /gene="yabA"
                     /locus_tag="VW006_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9380368.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10573..11442
                     /gene="rsmI"
                     /locus_tag="VW006_001540"
     CDS             10573..11442
                     /gene="rsmI"
                     /locus_tag="VW006_001540"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9380369.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11669..12187
                     /locus_tag="VW006_001541"
     CDS             11669..12187
                     /locus_tag="VW006_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9380370.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            12296..12944
                     /locus_tag="VW006_001542"
                     /pseudo
     CDS             12296..12944
                     /locus_tag="VW006_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(12933..>13285)
                     /locus_tag="VW006_001543"
     CDS             complement(12933..>13285)
                     /locus_tag="VW006_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9380371.1"
                     /translation="VSLDITVNYCRDMKLFKMSCRNIGQAGKILADSGYQGLMKIYPQ
                     VQTPRKSSKLKSLIAEDKAYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLR
                     MNLIAGIINHELGF"
BASE COUNT         4065 a         2236 c         3100 g         3884 t
ORIGIN      
        1 aacaaatgaa gaattagagc tgatacaagg tggagcagat tcatatggta aaaatcctaa
       61 tggtaggtac gattgggaaa tagaaccagt attaactctg ccggttcatg gattttgttc
      121 cagaggcacc tatgatttag gatatattgg aggaggcaat catctttgca aaggaagtgc
      181 tgcgagattt taagtaaaat ttattagtaa tatgaagaaa caagtggaga aagcagagaa
      241 tttaatatga aaaaacgggc tattcaaatt ttactagcat tgtccttaat tttttacaaa
      301 tcaacttggt tttggagagt tttcaatcac ctcgcaaagc cctatctacc agcaagtcgt
      361 gaattttttc agattctgct tttgatggag agcggagttc ttttcttagc ggtcatctat
      421 ctactggttt ttgcaggaaa gaaaattttt catttcaagt ggcagctgag gtacttcatc
      481 taccttttac tgggctacat catttcatat atgtctgact tcctcttttc gtatttcata
      541 tccctgtctt caaatcagat ttctttgaat gaaacgatag aaatgatggg gagacaggag
      601 ttcccttatt tcttgctcat cgtttgcttc atcgccccta ttgctgagga attgatttat
      661 cgaggtgtgc ttatgacaac ctttttcaaa aactcacctt ggtacggaga tgttttgctt
      721 tctgctatta ttttcggtta tattcatatc aattttgctt taactcctct tgcttttttc
      781 atttatgcta gtggaggtct gattttagct ctattgtatc gcatgactaa aaatctctac
      841 tatccaatac tagttcatat tctcatcaat atcactgcct tctgggatgt ttggttactc
      901 ctattttcag gaagttagct tactaaaaaa atgtcggaat tttccggcat tttctttttt
      961 cacaaatagt caacgttttt ctttccgata ctgaagtggt gtgtagccac ttattttttt
     1021 gaattgattt tgaaaataag attggcgtga gaaaggcaga tagtgaagat agttaagaag
     1081 aataggatgt tcttttttcc tttttggaaa acttctaaaa tatggtataa tgaaaagata
     1141 aagaagttgg gggtagaaga tgaacattca acaattacgc tatgttgtgg ctattgccaa
     1201 tagtggtact tttcgtgaag ctgctgaaaa gatgtatgtt agtcagccga gtctgtctat
     1261 ttctgttcgt gatttggaaa aagagttggg ctttaagatt ttccgtcgga ccagctcagg
     1321 gactttcttg acccgtcgtg ggatggaatt ttatgaaaaa gcgcaagaat tggttaaagg
     1381 atttgatatt tttcaaaatc agtatgccaa tcctgaagaa gaaaaagatg aattttccgt
     1441 tgctagccag cactatgact tcttaccacc aactattacg gccttttcag agcgctatcc
     1501 tgactataag aacttccgta tttttgaatc aactactgtt caaatattag atgaagtggc
     1561 gcaagggcat agtgagattg ggattatcta cctcaacaat caaaataaaa aggggattat
     1621 gcaacgggtt gaaaagttag gtctggaggt catcgaattg attcctttcc atacccatat
     1681 ttatctctgt gagggtcatc ctttagccca gaaagaggaa ttagtcatgg aggatttagc
     1741 ggatttacca acggttcgtt tcactcaaga gaaagacgag tacctttatt attcagagaa
     1801 ctttgtcgat accagcgcta gctcacagat gtttaatgtg acagaccgtg ccaccttgaa
     1861 tggtattttg gagcggacgg acgcctatgc gacaggttct ggatttttag atagtgacag
     1921 tgttaatggc attacagtta ttcgtctcaa ggataaccta gataaccgca tggtctatgt
     1981 taaacgtgaa gaagtggagc ttagtcaagc tgggactctc ttcgtagaag tcatgcaaga
     2041 atattttgat caaaagagga aatcatgaaa aaaagagcaa tagtggcagt cattgtactg
     2101 cttttaattg ggctggatca gttggtcaaa tcttatatcg tccagcagat tccactgggt
     2161 gaagtgcgct cctggatccc caatttcgtt agcttgacct acctgcaaaa tcgaggtgca
     2221 gccttttcta tcttacaaga tcagcagttg ttattcgctg tcattactct ggttgtcgtg
     2281 ataggtgcca tttggtattt acataaacac atggaggact cattctggat ggtcttgggt
     2341 ttgactctaa taatcgcggg tggtcttgga aactttattg acagggtcag tcagggcttt
     2401 gttgtggata tgttccacct tgactttatc aactttgcaa ttttcaatgt ggcagatagc
     2461 tatctgacgg ttggagtgat tattttattg attgcaatgc taaaagagga aataaatgga
     2521 aattaaaatt gaaactggtg gtctgcgttt ggataaggct ttgtcagatt tgtcagaatt
     2581 atcacgtagt ctcgcgaatg aacaaattaa atcaggccag gtcttggtca atggtcaagt
     2641 caagaaagct aaatacacag tccaagaggg tgatgtcgtc acttaccatg tgccagaacc
     2701 agaggtatta gagtatgtgg ctgaggatct tccgctagaa atagtctacc aagatgagga
     2761 tgtggctgtc gttaacaaac ctcagggaat ggttgtgcac ccgagtgctg gtcataccag
     2821 tggaacccta gtaaatgccc tcatgtatca tattaaggac ttgtcgggta tcaatggggt
     2881 tctgcgtcca gggattgttc accgtattga taaggatacg tcaggtcttc tcatgattgc
     2941 taaaaacgat gatgcgcatc tagtacttgc ccaagaactc aaagataaaa agtctctccg
     3001 caaatattgg gcgattgttc atggaaatct gcctaatgat cgtggtgtaa ttgaagcgcc
     3061 gattggccgg agtgaaaaag accgtaagaa acaggctgta actgctaaag ggaagcctgc
     3121 agtgacgcgt tttcacgtct tggaacgctt tggcgattat agcttagtag agttgcaact
     3181 ggagacaggg cgcactcatc aaatccgtgt ccacatggct tatatcggcc attcagtcgc
     3241 tggtgatgag gtctatggtc ctcgcaagac tttgaaagga catggacaat ttcttcatgc
     3301 caagacttta ggttttactc atccgagaac aggtaagacc ttggaattta aagcagatat
     3361 cccagagatt tttaaggaaa ccttggagag attgagaaag taagaatgaa aaagaaatta
     3421 actagtttag cacttgtagg cgctttttta ggtttgtcat ggtatgggaa tgttcaggct
     3481 caagaaagtt caggaaataa aatccacttt atcaatgttc aagaaggtgg cagtgatgcg
     3541 attattcttg aaagcaatgg acattttgcc atggtggata caggagaaga ttatgatttc
     3601 ccagatggaa gtgattctcg ctatccatgg agagaaggaa ttgaaacgtc ttataagcat
     3661 gttctaacag accgtgtctt tcgtcgtttg aaggaattgg gtgtccaaaa acttgatttt
     3721 attttggtga cccataccca cagtgatcat attggaaatg ttgatgaatt actgtctacc
     3781 tatccagttg accgagtcta tcttaagaaa tatagtgata gtcgtattac taattctgaa
     3841 cgtctatggg ataatctgta tggctatgat aaggttttac agactgctgc agaaaaaggt
     3901 gtttcagtta ttcaaaatat cacacaaggg gatgctcatt ttcagtttgg ggacatggat
     3961 attcagctct ataattatga aaatgaaact gattcatcgg gtgaattaaa gaaaatttgg
     4021 gatgacaatt ccaattcctt gattagcgtg gtgaaagtca atggcaagaa aatttacctt
     4081 gggggcgatt tagataatgt tcatggagca gaagacaagt atggtcctct cattggaaaa
     4141 gttgatttga tgaagtttaa tcatcaccat gataccaaca aatcaaatac caaggatttc
     4201 attaaaaatt tgagtccgag tttgattgtt caaacttcgg atagtctacc ttggaaaaat
     4261 ggtgttgata gtgagtatgt taattggctc aaagaacgag gaattgagag aatcaatgca
     4321 gccagcaaag actatgatgc aacagttttt gatattcgac aagacggttt agtaaacatt
     4381 tcaacttcct acaagccgat tccaagtttt caagctggtt ggcataagag ttcatatgga
     4441 aactggtggt atcaagcgcc tgattctaca ggagaatatg ctgtcggttg gaatgaaatt
     4501 aagggtgaat ggtattactt taaccaaacg ggtatcttat tacagaatca atggaaaaaa
     4561 tggaacaatc gttggttcta tttgacagac tctggtgttg ctgctaaaaa ttggaagaaa
     4621 atcgctggaa tctggtatta ttttaacaaa gaaaaccaga tggaaattgg ttggattcaa
     4681 gataaagagc agtggtatta tttggatgtt gatggctcta tgaagacagg ttggcttcaa
     4741 tataaggggc aatggtatta ctttgctcca tcaggggaaa tgaaaacggg ctgggtaaaa
     4801 gataaagaag cctggtacta tatggattct actggtatca tgaagacagg tgagatagaa
     4861 gttgctggac atcattacta tctagaagaa tcaggagcta tgaagcaagg ctggcttaaa
     4921 aaggcaaatg attggtattt ctacaagaca gatggttcac gagctatagg ttggattaaa
     4981 gacaaggata aatggtactt cttgaaagaa aatggtcaat tacttgtgaa tggtaagaca
     5041 ccagaaggct atactgtgga ttcaagtggt gcctggttag tggatgttcc gatcgagaaa
     5101 tctgctacaa ttaaaactac aagtcattca gaaataaaag aatccaaaga agtagtgaaa
     5161 aaggatcttg aaaataaaga aacgagtcaa catgaaagtg ttacaaattc ttcaactagt
     5221 caagatttga catcctcaac ttcacaaagc tctgaaacga gtgtaaacaa atcggaatca
     5281 gaacagtagt agaaaagaag gttttagggc cttctttttc ctatcaactc ttttctattt
     5341 cctgttattc atgttataat ggataaatat gaataatcgg agtgagacta tgaaatacaa
     5401 acggattgtc tttaaggtgg gtacttcttc tctgacaaat gaggatggaa gtttatcacg
     5461 tagtaaggta aaggatatta cccagcagtt ggctatgctg cacgaggctg gtcatgagtt
     5521 gattttggtg tcttcaggtg ccattgcggc tggttttgga gccttaggat ttaaaaagcg
     5581 tccgactaaa attgctgata aacaggcttc agcagcggta gggcagggac ttttgttgga
     5641 agaatatacg accaatcttc tcttgcgtca aatcgtttct gcacaaatct tgctgaccca
     5701 agatgacttt gtggataagc gtcgttataa aaatgcccat caggctttgt cggttttgct
     5761 caaccgtggg gcaattccta tcatcaatga gaatgatagt gtcgttattg atgagctcaa
     5821 ggttggggac aatgacactc taagtgctca agtagcggcg atggtccaag cagacctttt
     5881 agttttcttg acagatgtgg acggtctcta tactggaaat cctaattcag atccaagagc
     5941 caaacgcttg gagagaatcg agaccatcaa tcgtgagatt attgatatgg ctggtggagc
     6001 tggttcgtca aacggaactg ggggtatgtt aaccaaaatc aaggctgcaa ctatcgcgac
     6061 ggaatcagga gttcctgttt atatctgctc atccttgaaa tcagattcca tgattgaggc
     6121 ggcagaggag accgaggatg gttcttactt tgttgctcaa gagaaggggc ttcgtaccca
     6181 gaaacaatgg cttgccttct atgctcagag tcaaggttct atttgggttg ataaaggggc
     6241 tgcggaagct ctctctcaac atggaaagag tcttctctta tctggtatcg ttgaagcaga
     6301 aggagtcttt tcttacggtg atatcgtgac agtatttgat aaggaaagtg gaaaatcact
     6361 tggaaaagga cgcgtgcaat ttggagcatc tgctttggag gatatgttgc gttctcaaaa
     6421 agccaagggt gtcttgattt accgtgacga ctggatttcc attactcctg aaatccaact
     6481 actttttaca gaattttaga ggtaaactat ggtgagtaga caagaacaat ttgaacaggt
     6541 acaggctgtt aaaaaatcga ttaacacagc tagtgaagaa gtgaaaaacc aagccttgct
     6601 agccatggct gatcacttag tggctgctac tgaggaaatt ttagcggcta atgccctcga
     6661 tatggcagcg gctaagggga aaatctcaga tgtgatgttg gatcgtcttt atttggatgc
     6721 agatcgtata gaagcgatgg caagaggaat tcgtgaagtg gttgccttac cagatccaat
     6781 cggtgaagtt ttagaaacaa gtcagcttga aaatggtttg gttatcacaa aaaaacgtgt
     6841 agctatgggt gtcatcggta ttatctatga aagccgtcca aatgtgacgt ctgatgcggc
     6901 tgctttgact cttaagagtg gaaatgcggt tgttcttcgt agtggtaagg atgcctatca
     6961 aacaacccat gccattgtca cagccttgaa gaagggcttg gagacgacta ctattcatcc
     7021 aaatgtgatt caactggtgg aggatactag ccgtgaaagt agttatgcta tgatgaaggc
     7081 caagggctat ctagaccttc tcattcctcg tggaggagct ggcttgatca atgcagtggt
     7141 tgagaatgcg attgtacctg ttatcgagac agggactggg attgtccatg tctatgtgga
     7201 taaggatgca gacgaagaca aggcgctgtc tatcatcaac aatgctaaaa ccagtcgtcc
     7261 ttctgtttgt aatgccatgg aggttctgct ggttcatgaa aacaaggcag caagcatcct
     7321 tcctcgcttg gatcaaatgc tggttgcaga tcgaaaagaa gctgggttgg aaccaattca
     7381 attccgccta gatagcaaag caagccagtt tgtttcaggt caagctgctc aagcacaaga
     7441 ctttgatacc gagtttttag actatattct agctgttaag gttgtgagca gtttagaaga
     7501 agcggttgcg catattgaat cccacagtac ccatcattcg gatgctattg tgacggaaaa
     7561 tgctgaagct gcagcatact ttacagatca agtggactct gcagcggtgt atgttaatgc
     7621 ctcaactcgt ttcacagatg gaggacaatt tggtcttggt tgtgaaatgg ggatttctac
     7681 tcagaaattg cacgcgcgtg gtccaatggg cttgaaagag ttgaccagct acaagtatgt
     7741 ggttgctggt gatgggcaga taagggagta agagatgaag attggattta tcggtttggg
     7801 gaatatgggt gctagcttgg caaaatctgt cttgcagact aggacgtcag atgagattct
     7861 ccttgccaat cgtagtcaag ctaaggtaga tgctttcatt gcagactttg gtggtcaggc
     7921 ttccagcaat gaagaaatgt ttgcagaagc agatgtgatt tttctaggag ttaagcctgc
     7981 tcagttttct gaactgcttt ctcaatatca gaccatcctt gaaaaaagag aaagtcttct
     8041 tttgatttcg atggcagctg gattgacctt agaaaaacta gcaagtctta tcccaagcca
     8101 acaccgaatt attcgtatga tgcctaatac ccctgcttct atcgggcaag gagtgattag
     8161 ttatgccttg tctcctaatt gcagggctga ggacagtgag atcttttgtc agcttttagc
     8221 caaggctggt ctcttggttg aattaggaga aagcttaatc aatgcagcga caggtcttgc
     8281 aggttgtgga ccagcctttg tctatctctt tattgaggcc ttggcagatg caggtgttca
     8341 gacgggatta ccacgagaaa cagccttgaa aatggcagcc caaactgtgg taggagctgg
     8401 gcaattggtc ctagaaagcc agcaacatcc tggagttttg aaagatcaag tttgtagtcc
     8461 aggcggttcg actattgctg gcgtagcaag cttagaagcg catgcttttc gaggcatagt
     8521 catggaggca gttcatcaag cctataaacg aacacaagaa ctaggtaaat aagaggtagc
     8581 tttgactgcc tcttttatgg tggctgaaat gagaagacac aaaaagattg tcacaaactc
     8641 ctattttttt gatagaatag aagtagtaaa aaagaaatga gttagacatg tcaaaaggat
     8701 ttttagtctc tcttgaggga ccagagggag caggcaagac cagtgtttta gaggctctgc
     8761 taccaatttt agaggaaaaa ggagtagagg tgctgacgac ccgtgaacct ggcggagtct
     8821 tgattgggga gaagattcgg gaagtgattt tggatccaag tcatactcag atggatgcta
     8881 aaacagagct acttctctat attgccagtc gcagacagca tttggtggaa aaagttcttc
     8941 cagcccttga agctggcaag ttggtcatca tggatcgttt tatcgatagt tctgttgcct
     9001 atcagggatt tggtcgtggc ttagatattg aagccattga ctggctcaat cagtttgcga
     9061 cagatggcct caaacccgat ttgacactct attttgacat cgaggtggaa gaagggctgg
     9121 ctcgtattgc tgctaatagt gaccgcgagg ttaatcgttt ggatttggaa gggttggact
     9181 tgcataaaaa agttcgtcaa ggctaccttt ctcttctgga taaagaggga aatcgcattg
     9241 tcaagattga tgctagtctc cctttggagc aagttgtgga aactaccaat gctgtcttgt
     9301 ttgacggaat gggcttggcc aaatgaaaca agatcaacta aaggcttggc aaccagctca
     9361 gtttgaccgt tttgtccgta tcttagaaca agaccagctc aatcacgcct atctcttttc
     9421 aggtttcttt ggaagcttgg aaatggcgca atttttagct aagagccttt tttgtacgga
     9481 taaagttggc gtcttaccat gtgagaaatg ccgaagttgc aagctgattg aacaggaaga
     9541 gtttccagat gtcaccttga ttaagccagt caatcaggtc atcaagacag aacgtattcg
     9601 ggaattggtg ggacagtttt ctcaagcagg gattgaaagc cagcaacagg tctttatcat
     9661 cgagcaagcg gataaaatgc atcccaacgc agccaattct ctgctcaagg tcatcgaaga
     9721 accccagagt gaagtttata ttttcttctt gactagcgat gaggaaaaga tgttaccgac
     9781 aatccgaagt cggactcaga tcttccactt taaaaagcaa gaagaaaaac ttatcttact
     9841 cttagaacaa atgggacttg ttaagaaaaa agcgactctt ttagctaagt ttagtcaatc
     9901 gcgagctgaa gcagaaaagt tggcacatca ggcaagtttt tggaccttgg tcgatgaaag
     9961 tgaacgcctg ctgacttggt tagtagctaa gaaaaaagaa agttatctac aggttgccaa
    10021 attagccaac ttggcagatg ataaggaaaa acaggatcag gttttacgga ttcttgaagt
    10081 tctctgtggg caggacctct tgcaggtaag agtaagagtg attctacaag atttactaga
    10141 agctagaaaa atgtggcaag ctaatgtcag ctttcaaaat gccatggaat atctggtctt
    10201 gaaagaaata taaactcaaa aatgaatgat aaagaaagga aagggctgtt ttatggacaa
    10261 aaaagaatta tttgacgcgc tggatgattt ttcccaacag ttattggtaa ccttggccga
    10321 tgtggaagcc atcaagaaaa atctcaagag cctggtagag gaaaatacag ctcttcgttt
    10381 ggaaaattct aagttgcgag aacgcttggg tgaggtggaa gcagatgctc ctgtcaaggc
    10441 caagcatgtt cgtgaaagtg tccgtcgcat ttaccgtgat ggatttcacg tatgtaatga
    10501 tttttatgga caacgtcgag agcaggacga ggaatgtatg ttttgtgacg agttgctata
    10561 cagggagtag gcatgcagat tcaaaaaagt tttaaggggc agtctcccta tggcaagctg
    10621 tatctagtgg caacgccgat tggcaatcta gatgatatga cctttcgagc tatccagacc
    10681 ttgaaagaag tagattggat tgctgctgag gatacgcgca atacaggtct tttgctcaag
    10741 cattttgaca tttccaccaa gcagatcagt tttcatgagc acaatgccaa ggaaaaaatt
    10801 cctgatttga ttggtttctt gaaagcaggg caaagtattg ctcaggtctc tgatgccggt
    10861 ttgcctagca tttcagaccc tggtcatgat ttagttaagg cagctattga ggaagaaatt
    10921 gcagttgtga cagttccagg tgcctctgca ggaatttctg ccttgattgc cagtggttta
    10981 gcgccacagc cacatatctt ttacggtttt ttaccgagaa aatcaggtca gcagaagcaa
    11041 ttttttggct tgaaaaaaga ttatcctgaa acacagattt tttatgaatc acctcatcgt
    11101 gtagcagaca cgttggaaaa tatgttagaa gtctacggtg accgctccgt tgtcttggtc
    11161 agggaattga ccaaaatcta tgaagaatac caacgaggta ctatctctga gttattagaa
    11221 agcattgctg aaacgccact caagggcgaa tgtcttctca ttgttgaggg tgccagtcag
    11281 ggtgtggagg aaaaggacga ggaagacttg ttcgtagaaa ttcaaacccg catccagcaa
    11341 ggtatgaaga aaaatcaagc tattaaggaa gtagctaaga tttaccagtg gaataagagt
    11401 caactctacg ctgcctacca cgactgggaa gaaaaacaat aaagggagat aggatgtaat
    11461 aattctgtct gtttctgttt aacttaatta gtgatgataa tataaagatg tatcacttgg
    11521 tatagaagct ttggtattaa gttttttatt aagcccatac ggaataccga tggttggagc
    11581 agcagttata gcgttcttag aaggtataaa tagaaaaata aggtcatttt aaatcaaagg
    11641 attgataaat cagaaagaag gtgatttttt gcgaacatac gaaaataaag aagaactaaa
    11701 agctgagata gagaaaacat ttgagaaata tattttagaa tttgataata ttccagaaaa
    11761 tttaaaagat aagagagctg atgaagttga cagaactcca gcagaaaacc ttgcttatca
    11821 ggttggttgg accaacttgg ttcttaaatg ggaagaagat gaaagaaagg ggcttcaagt
    11881 aaaaacacca tcggataaat ttaaatggaa tcaacttggt gaattatatc agtggttcac
    11941 agatacctac gctcatttat ctctgcaaga gttgaaagca aaattaaatg aaaatattaa
    12001 ttctatctct gcaatgattg attcgttgag tgaggaagaa ttatttgaac cgcatatgag
    12061 aaagtgggct gatgaagcga ctaaaacagc gacttgggaa gtgtataagt ttattcatgt
    12121 aaatacggtt gcaccttttg gaactttcag aactaaaatc agaaaatgga agaagatagt
    12181 attataaatt atatttttaa ctttaaaaaa tttcataaaa atggttacca aaggcgatag
    12241 aagaaaaact atcgtctttt tctttgcaaa tttttaagaa gggaggtgat cttgcatgga
    12301 ctttgaatat ttttataaca gagaagcgga aagatttaac ttcttaaaag taccggagat
    12361 attagttgat agagaagaat ttcggggctt atcagcagaa gcaattatcc tttattccat
    12421 acttcttaaa cggacaggaa tgtcatttaa gaataactgg atagacaagg aaggcagagt
    12481 atttatctat tttactgtcg aagaaattat gaaaagaaga aatatctcaa agccaactgc
    12541 cataaaaaca ttagatgagc ttgatgtaaa aaaaaggaat aggactgatc gaaagagtaa
    12601 ggcttggact tggtaagccg aacatcattt atgttaaaga ctttatgagt atatttcagg
    12661 taaaagaaaa tgacttacaa aagtcaaaaa acttaacttc agaagtaaaa gattttaacc
    12721 tcagaagtaa agaaaatgaa cttcaagagg ttaagaacct tgactctaac tatatagaga
    12781 ataataagag taagtatagt aagagagaat atagttttgg tgaaaacgga cttggaacat
    12841 ttcaaaatgt gtttttagct gttgaagata tatcggattt acaaatcata atgaactcac
    12901 agcttgagaa ttacattaga cttcctgcaa aactagaatc ctagttcatg attgataata
    12961 ccagcaatca aattcattcg taatccgaag cgtttacgat gatttcgata ggttgttgaa
    13021 aacattttaa acgtttttac tttggcaaag atgttctcaa ccttgcttct ttccttagat
    13081 agcgcatggt tataggcttt atcttcagct attagcgact tgagtttgct ggatttacgt
    13141 ggagtttgta cttgaggata tatcttcatg agcccttgat aaccactgtc agccaagatt
    13201 ttaccagctt gtccgatatt tctgcaactc attttgaaca acttcatatc acgacaatag
    13261 ttcacagtga tatccaaaga aacaa
//
LOCUS       DAVKFH010000047        13060 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKFH010000047 DAVKFH010000000
VERSION     DAVKFH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13060
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.047"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            255..647
                     /locus_tag="VW006_001544"
     CDS             255..647
                     /locus_tag="VW006_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380372.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            669..1460
                     /locus_tag="VW006_001545"
     CDS             669..1460
                     /locus_tag="VW006_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380373.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1453..2094
                     /locus_tag="VW006_001546"
     CDS             1453..2094
                     /locus_tag="VW006_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380374.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            2098..2730
                     /locus_tag="VW006_001547"
     CDS             2098..2730
                     /locus_tag="VW006_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9380375.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2758..3045
                     /locus_tag="VW006_001548"
     CDS             2758..3045
                     /locus_tag="VW006_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380376.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            3191..5704
                     /gene="secA"
                     /locus_tag="VW006_001549"
     CDS             3191..5704
                     /gene="secA"
                     /locus_tag="VW006_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9380377.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5785..6816
                     /locus_tag="VW006_001550"
     CDS             5785..6816
                     /locus_tag="VW006_001550"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9380378.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6818..7849
                     /locus_tag="VW006_001551"
     CDS             6818..7849
                     /locus_tag="VW006_001551"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9380379.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7888..8256
                     /locus_tag="VW006_001552"
     CDS             7888..8256
                     /locus_tag="VW006_001552"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9380380.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8246..9349
                     /locus_tag="VW006_001553"
     CDS             8246..9349
                     /locus_tag="VW006_001553"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9380381.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9368..11383
                     /gene="recG"
                     /locus_tag="VW006_001554"
     CDS             9368..11383
                     /gene="recG"
                     /locus_tag="VW006_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9380382.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPGWRMIALHLEKKEHLD"
     gene            11700..12680
                     /locus_tag="VW006_001555"
     CDS             11700..12680
                     /locus_tag="VW006_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9380383.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         3827 a         2411 c         2962 g         3860 t
ORIGIN      
        1 tcaaaaagga gggaaaaagt catgggcttt aaaaaatatt tgaagaattt accgaaaaac
       61 tctggatttt tgatttggag ttggattcaa cttatctggt ttgaaacatg gttttgggga
      121 taaagaaatt agagtctata tataataagt aaaggaggaa tttgtatgat ataccatcgt
      181 ttagaataag tctatattta aaatagaagt tataaggaat cttcttgaat aaaaaatgaa
      241 aggaaacaga aaaaatgaaa attaagaaat tattgaaaat ggttattcct gttttaatga
      301 taagtgctgt tggaactact ttcgtagaag caaatcagat aggtgctttt agtaattttg
      361 ttattactac cagttataag agaacaggtt atttgactaa agaaaatgaa ggtgcggaat
      421 atatcatgaa cttaaatcct tgtagaaatt tacatcccat gactgttaag catcgtatag
      481 taaattctaa tggagaagct cgtagtggag aatcgttaac aacctgtggt actcgttcaa
      541 ctcatggtaa ttgggcaacg gttggctatg tttatgcagc agatatggca agacaaaatt
      601 ggtgggattt atcagctgcc atttcaggta gttggtcacc aaattaattc gtaaagtagg
      661 tataatttat gaagattcct ctcttaactt tggcaaggca taaatttgtt tatgtcttgc
      721 ttactttgct ttttcttgct ttggtttatc gtgacgtttt gatgacttat ttcttttttg
      781 atattcatgc gcccgatcta gctaaattcg atggacaagc aattaaaaat gacttattaa
      841 aatcagcatt agattttcgt attctccagt tcaatctagg tttttatcaa tcatttatta
      901 ttccaatcat cattgttttg ctaggttttc aatatattga gctgaaaaat aaagttttac
      961 gattgagtat tggaagagaa gtgagctatc cagggttaaa aagaaagttg actttgcaag
     1021 ttgcaagtat cccttgtttg atatatttag tgactgtgct gacaattgca attataacct
     1081 atttccttgg gactttttct cctcttggat ggaattctct attttctgat ggaagtggtt
     1141 tacaaagact cctagatgga gagataaaaa gctatttgtt ctttacttgt gtcctgctaa
     1201 tcggtatttt catcaatgca atctattttt tacaaatagt tgattatgtg gggaatgtga
     1261 ctcgttcggc aatcacctat ttgatgtttc tttggcttgg ttctatgctg ctctatagtg
     1321 ccttgcctta ctatatggtt cctatgacga gtttgatgca agctagctat ggggatgtaa
     1381 gtttgatgaa actctttact ccttatatcc tttatattgt cccttacatg gtgcttgaaa
     1441 aatatgaaga taatgtttaa gaattttaac aatattttgc taaagagaaa gattgtttta
     1501 ctacttcgta tagttctgat gatgattttg ataaaccatc tattgtcaac agcggttcaa
     1561 aagcaggatg ctgttatctt tttcaagaga gaattgattt caattttttc ctataatgac
     1621 tattctgaag cgaatttaga aatccccaaa ctcttgttaa acctttcgct tttcatggta
     1681 ggatggctct ctgtcatttt acttgaaagt gatttggcag accattacca tcacttgatt
     1741 cgctatcaat caagctcctt tttcgattat acaaggaaac gattggttgt catttctaaa
     1801 ttttttactc aagatttgtt tgtctggttc cttggtttac ttccgctagg aattcatttc
     1861 aaaacagtcg cacttttctt tttacttgct cagttaatga tgttgtactt actactgtct
     1921 tatctgatag cactgattag tgcgggcgct ggtttttcct tttttctcta ttttttagta
     1981 tttgtgggac aagaatggat gatggatcat attgtaacag tgtatttact actcttaagt
     2041 ttattagtta tgttgattgt tagtcgcttg gaagagaaat ttaagaaagg ataaacgatg
     2101 agacttgaaa ttataaatgg acagaaaatt tatgggaaaa gacctatttt aaatcagttg
     2161 aatttggtgt ttcaatcagg aaaaatttat ggacttaaag gtgataatgg atctggcaag
     2221 acggttcttt taaaggtact tgctggctat attaagcttg acaaaggaaa agttcttcaa
     2281 gacgggaaag tctacgggat aaaaaatcat tatattcagg atgcaggaat tttaattgaa
     2341 aaagtcgagt ttttatctca tttatccctg agagaaaatt tggaactgtt aaggtatttt
     2401 tcatctaaag ttacggaaaa aagaattgcc tattggattc aatactatga tttacaggaa
     2461 tttgaagacg ttgaataccg tcatttatcc ttgggaacaa agcaaaaaat ggccttgatt
     2521 caagccttta tttcctctcc ttctatactc tttctcgatg aacctatgaa tgctttggat
     2581 gagaagagtg tgaggttaac caaacaggtc attttatctt acctgaaaaa agaaaatggt
     2641 ctggttatcc tgacgtcgca catatcggaa gatatttcag acctttgtac agatgtatta
     2701 gttgtcgaaa atggacatat acaaatgtaa aggatataca atcctaggag atggcttatg
     2761 gcacatctaa aatcatttat tacacgctat tccaaggttt atattggttt agttctgctg
     2821 atctggctgt ctttcttctt tatcccttgg gatagagcga ttctgggggt aaggattgac
     2881 atcttcatca tacagaaaat cttgctagct tttggaattc tgtccattct catggccttg
     2941 ctgtccaaga aagtcagtct ctttgttttt ggactgattt gctgtctttc tctttggatt
     3001 aacttattta tcacatttgc cattttgccg atttttggca attaaacagt cataaaagtc
     3061 ggagaggtta tcttgaaaac taacctcttt ttccttttca aaatggggat tcttccttga
     3121 aaataatcag taattgtgct aaaattaaag gaacattcta aaatattcgg aatttaaagt
     3181 aaggaaaaac atggctaata ttttaaaaac aattatcgaa aatgataaag gagaaatccg
     3241 tcgtctggaa aagatggctg acaaggtttt caaatacgaa gaccaaatgg ctgctttgac
     3301 tgacgaccaa ctaaaagcaa aaacagttga atttaaggaa cgttatcaaa atggagaatc
     3361 actggattca ttgctttacg aagcatttgc ggttgtccgt gaaggtgcca aacgtgtcct
     3421 aggtctcttc ccttataagg ttcaggtcat gggggggatt gttcttcacc atggtgacgt
     3481 gccagagatg cgtacagggg aagggaaaac cttaactgcg accatgccgg tatacctcaa
     3541 tgccctttca ggtaaagggg ttcacgtagt tacggttaat gaatacctgt cagaacgtga
     3601 cgcgactgag atgggtgaat tgtactcttg gcttggtttg tcagtaggga ttaacttggc
     3661 taccaaatct ccaatggaga aaaaagaagc ctatgagtgt gatattactt actcaactaa
     3721 ctcagaaatc ggatttgact accttcgtga caacatggtc gttcgcgctg aaaacatggt
     3781 acaacgtccg cttaactatg ccttggtcga tgaggttgac tctatcttga ttgacgaggc
     3841 tcgtacacct ttgattgtat caggtgccaa tgcggttgaa accagtcagt tgtatcacat
     3901 ggcagaccac tatgtaaaat ctttgaacaa agatgactac atcatcgatg tgcagtctaa
     3961 gactattggt ttgtctgatt cagggattga cagggctgaa agctacttca aacttgaaaa
     4021 cctctatgac atcgaaaacg tggctttgac ccactttatc gataacgccc ttcgtgccaa
     4081 ctacatcatg cttctcgata ttgactatgt ggtgagcgaa gagcaagaaa tcttgattgt
     4141 cgaccaattt acaggtcgta ccatggaagg tcgtcgttat tctgatggat tgcaccaagc
     4201 tattgaagcc aaagaaggtg tgccaatcca ggatgaaacc aagacatctg cctcaatcac
     4261 gtaccaaaac cttttccgta tgtacaaaaa attgtctggt atgacgggta caggtaagac
     4321 tgaggaagaa gaatttcgtg aaatctacaa cattcgtgtt attccaatcc caacaaaccg
     4381 tcctgttcaa cgtattgacc actcagacct tctttatgca agtatcgaat ctaagtttaa
     4441 agcggttgtc gaagacgtta aggctcgtta ccaaaagggt caacctgtct tggttggtac
     4501 agtagcggtt gaaactagtg actacatttc taagaaattg gttgcagctg gtgttcctca
     4561 cgaagtcttg aatgccaaaa accactatag agaagcccaa atcatcatga atgctggtca
     4621 acgtggtgcc gttaccatcg caaccaacat ggcgggtcgt ggtaccgaca tcaagcttgg
     4681 tgaaggtgtt cgtgaacttg gaggactttg tgttattggt acagaacgtc atgaaagtcg
     4741 tcgtatcgat aaccagcttc gtggacgttc aggtcgtcaa ggagatccag gtgagtcaca
     4801 attctaccta tctcttgaag atgatttgat gaaacgtttt ggttctgaac gcttgaaggg
     4861 aatctttgaa cgtctcaaca tgtctgaaga ggccattgag tctcgcatgt tgacgcgtca
     4921 ggttgaagca gctcagaaac gtgtcgaagg aaataactac gatacccgta aacaagtcct
     4981 tcaatacgat gatgtcatgc gtgaacaacg tgagattatc tatgctcaac gttacgatgt
     5041 catcactgca gatcgtgact tggcacctga aattcagtct atgatcaaac gcacgattga
     5101 acgtgtcgtt gatggtcatg cgcgtgccaa acaagatgaa aaactagagg caattttgaa
     5161 ctttgctaag tacaacttgc ttcctgaaga ttctattacg atggaagact tgtcaggctt
     5221 gtctgataag gccatcaagg aagagctttt ccaacgttcc ttgaaggttt acgatagtca
     5281 ggtttcaaaa ctacgcgatg aagaagcagt taaagaattc caaaaagttt tgattctacg
     5341 agtggtggat aacaagtgga cagatcatat cgatgccctt gatcaattgc gtaacgcggt
     5401 tggacttcgt ggctatgctc agaacaaccc tgttgttgag tatcaggcag aaggtttccg
     5461 tatgtttaat gatatgattg gttcgattga gtttgatgtg acacgcttga tgatgaaagc
     5521 acaaattcat gaacaagaaa gaccacaggc agaacgtcat atcagtacaa cagcgactcg
     5581 caatatcgct gctcaccaag caagtatgcc agaagatttg gatttgagcc agattggacg
     5641 caatgaactt tgcccatgtg gttctggtaa gaaatttaaa aactgtcacg gtaaaagaca
     5701 ataaaatgag atagtttaga ggcggatatc ttgtgaaaag taaattttta ctgggtatcc
     5761 gtttgcttta taaggagatg agttatggta tttacagcaa aaagctctaa aataaatata
     5821 gaagaagttc gtgccttgtc aaaattagaa ggtcaggctt tggagaggaa atcacagcga
     5881 gatcaagagc tagaagccat tatacgtgga gaagaccagc gaattctctt ggtaatcggg
     5941 ccatgctcat ctgacaacga agaagctgtc cttgaatacg ctaagcgttt ggcagtccta
     6001 caagaagaag tggcagatcg tatctttatg gttatgcgtg tttatactgc caaaccccgt
     6061 accaacggag atggctataa gggcttgatt caccagccta acgcgacaga agcgcctagt
     6121 cttatcaatg gaatcaaagc cgttcgccat cttcactatc gtgtcatcac agaaacaggg
     6181 atgacaactg ctgatgaaat gctttatcct gaaaaccttc cgcttgtaga tgatttgatt
     6241 tcttacatgg cagttggtgc ccgttcagtt gaagaccagc aacaccgctt tgtggcaagt
     6301 ggggcaggat tttctactgg ttttaaaaat ccaacctctg gaaatctcaa tgtcatgttt
     6361 aatgggattt atgctgctca aaacaaacaa agtttccttt tcttaggaaa agaagtagaa
     6421 acaactggga acccgctttc acacgctatt cttcgtggtg ctcttaatga gtatggaaaa
     6481 aatattccca actactatta tgacaattta attgatacca ttgcccagta tgagaaaatg
     6541 ggcttggaaa atccttttat catcattgat accaatcatg acaattctgg taagcagtat
     6601 attgaacaga tccgaattgt ccgccagacc ttgattaacc gtgcttggaa tgaaaaaatt
     6661 aagcagttcg ttcgtggttt tatgattgag tcttatctgg aagatggtcg acaaaatgag
     6721 ccagaagtat ttggtaagtc tatcacagac ccttgcctgg gttgggataa cacagaagct
     6781 cttgtcagag aaatttacaa aacgttagga gaataagatg gcatttattg aaaaaggtca
     6841 agaaatcgat atggaagtca tcaaggctga aacccaattg tctgcggaag ccttgagact
     6901 caaggaaagc cgtgacaggg aattggcaga tattatttca ggggaagatg accgtattct
     6961 cttggtgatt ggtccttgct cttctgataa tgaagaggcg gtcttggaat atgctcgccg
     7021 tttatctgcc ttgcaaaaga aggtagcgga taagattttc atggtcatgc gcgtgtatac
     7081 tgctaagcct cgtaccaatg gagacggcta taaaggatta gttcaccagc cagatacttc
     7141 taaggctcca agcctgatta atggcttgca ggctgtgcgc cagttgcact accgcgtgat
     7201 tacagagact ggtttgacaa cggcagatga gatgctttat ccgtcaaatc tgatcttggt
     7261 ggatgacttg gtcagctacc atgccgttgg agctcgttct gtggaagacc aagagcaccg
     7321 ctttgtggct tctgggattg atgcaccagt agggatgaaa aatccaacct caggaaattt
     7381 gggtgttatg tttaacgcca tctatgctgc tcaaaacaag caaaccttcc tttatcatgg
     7441 gcaggaagtt gagacatcag gtaatccttt ggcccatgtt atcctccgtg gagcagtcaa
     7501 cgagtatggc aattatatgc cgaattacta ctatgaaaat ctactccaag ccattgaacg
     7561 ctatgaaacc atgggacttg aaaatccttt tatcctcatt gacaccaacc atgataactc
     7621 aggcaagcaa tatatggagc agattcgaat tgttcgccag accttgcaga atcgtgattg
     7681 gaatgagaaa attaaaaaga cggttcgagg atttatgatt gaatcttacc tagcagatgg
     7741 tcgtcaaaac caaccagaga tctttggttg ctctattact gacccttgcc taggttggga
     7801 aaatacagag gccttggtag aagagattta tgttaccttg acaaaataag tgaaaaggat
     7861 ggagttgggg aatctcaact ccttttgatg agaatgatag ttggacacgg aattgacatc
     7921 gaagaattgg cttcgataga aagcgcagtt acacgacatg aaggatttgc taagcgtgta
     7981 ctgaccgctc aggaaatgga gcgcttcacc agtctcaaag gacgcaggca aatagaatat
     8041 ttagctggtc gctggtcggc taaggaggcc ttttccaagg ctatgggaac gggcattagc
     8101 aagctcggtt ttcaggattt ggaagtcttg aacaatgaac gtggggcgcc ttattttagt
     8161 caggcaccat tttcaggaaa gatttggctg tctatcagcc acaccgatca gtttgtgaca
     8221 gccagtgtca ttttggagga aaatcatgaa agctagtcca catagaccaa ccaaggctct
     8281 gattcatctg ggagctattc gacaaaatat tcagcaaatg ggggctcata tccctcaagg
     8341 aacgctcaag ttggctgtgg ttaaggccaa tgcttatggt catggagctg ttgccgttgc
     8401 caaggcaatt caagatgatg ttgatggctt ttgcgtttcc aatatcgatg aagccattga
     8461 actcagacaa gctggactca gcaagccaat cctcatttta ggagtttctg aaatcgaagc
     8521 tgttgctcta gctaaagaat atgacttcac cttgacagtg gctggactgg agtggattca
     8581 agcactctta gataaggaag tggacctaac tggattgaca gtccacctca agattgattc
     8641 agggatggga cggattggtt ttagagaggc aagtgaggtt gagcaggctc aagatttgct
     8701 ccaacaacac ggtgtttgtg ttgaaggaat ctttacccac tttgctactg ctgatgagga
     8761 atcagatgac tattttaatg cccagttaga acggtttaaa actattttag ctagtatgaa
     8821 ggaagttcca gagctggttc atgctagcaa ttctgcaacg actctttggc atgtagagac
     8881 tattttcaat gcggttcgta tgggagatgc catgtatggc ctcaatccaa gtggagcggt
     8941 cttggatttg ccttatgatt tgataccggc cttgaccttg gagtctgctc tggttcatgt
     9001 caagacagtt ccagctggag cttgcatggg ctatggagca acttatcaag cggatagcga
     9061 gcaagtcatc gcgaccgtgc caatcgggta tgcagatgga tggacaagag acatgcaaaa
     9121 tttctctgtc ttggtagatg gccaagcttg cccaattgtc ggcagggttt cgatggacca
     9181 aatcactatt cgattgccta agctttatcc gctaggaacc aaggtaacct tgattggctc
     9241 caatggggat aaggaaatca ctgcaactca ggtagcgacc taccgcgtaa ccattaacta
     9301 tgaggtggtt tgcctcctca gcgaccgtat tccgagagaa tattattaga aaagaaagga
     9361 gtggagcatg aatctacatc aacccttgca tgtcttgcct ggtgtgggac caaagtcagc
     9421 agaaaaatac gccaaactag gaattgaaaa cttgcaagat ctcttgctct actttccttt
     9481 ccgttatgaa gacttcaaaa ccaagcaggt gctggagctg gaagacggtg agaaggcagt
     9541 tctttctggt caggtagtga ctcctgctag tgtccagtat tatggtttca agcgcaatcg
     9601 cctgcgtttt agtctcaagc agggagaggt cgtttttgcg gtgaatttct ttaaccagcc
     9661 ctatctggct gataaaatag agttgggagc aacccttgct gtctttggaa aatgggaccg
     9721 cgctaaggct agtctgactg ggatgaaggt tctggctcag gtagaagatg acctccagcc
     9781 tgtctatcgt ctggctcagg gaatcagtca ggccagtctg gtcaaggtca tcaagacggc
     9841 ttttgatcag ggactggacc tcttgataga agaaaatctg ccccagtctt tactagacaa
     9901 atacaaactc atgtcccgtt gtcaggcagt ccgagctatg cattttccaa aggatttggc
     9961 agaatacaag caggctcttc gccgtataaa gtttgaggaa ctcttttatt tccaaatgca
    10021 gctgcagatg ctcaagtctg aaaatagagt tcagggaagt ggtctggttc tgaattggtc
    10081 tcaggaaaaa gtgacagcag ttaaagtaag tcttcctttt gccctgaccc aagctcagga
    10141 aaagagtttg caggaaattt taactgatat gaagtccgac caccacatga atcgtctcct
    10201 acaaggggat gtggggagtg gaaaaacggt agtcgctggc ttggccatgt ttgcggcagt
    10261 gacagcaggt tatcaggctg ccctaatggt accaacagaa atcctcgcag agcaacactt
    10321 tgagagttta cagaaccttt ttcccaattt gaaactggct ctcttgatag gttccttgaa
    10381 agctgcagaa aagagagagg tcttggagac cattgccaag ggtgaggctg atttgattat
    10441 cggaactcac gctctgatac aagatggggt ggagtatgct cgtcttgctt tgattattat
    10501 cgatgagcag caccgttttg gtgtagggca aaggcgtatt ttacgggaaa aaggtgacaa
    10561 tccagatgtc ctcatgatga cggcgactcc cattccacgg acgcttgcca tcacagcctt
    10621 tggagatatg gatgtttcca ttatcgacca gatgccagca ggtcggaagc ctattgtgac
    10681 gcgctggatc aaacatgagc aactacctca ggtcttgact tggttagagg gggaaattca
    10741 aaaaggttcc caagcctatg tcatctctcc tttgattgaa gaatcagaag ctctagattt
    10801 gaaaaatgcc attgccttat cagaggagtt gacgactcat tttgcaggca aggcagaggt
    10861 ggctcttcta catggtagga tgaagagtga cgaaaaagac cagatcatgc aggatttcaa
    10921 ggagagaaag acggatattc tggtttcgac gacggttatt gaggttgggg tcaacgttcc
    10981 caatgcgact gtcatgatta tcatggatgc cgatcgcttc ggtctcagtc aacttcacca
    11041 gcttagaggt cgtgtcggtc ggggggacaa gcagtcctac gctgttctcg ttgctaatcc
    11101 caagacggat tctgggaaag accgcatgcg tatcatgaca gaaacgacca atggatttgt
    11161 ccttgcggag gaagatttga aaatgcgtgg ttctggtgag atttttggaa ccagacagtc
    11221 aggacttcca gagttccaag tggctgatat tatcgaagat tttccgattt tagaagaagc
    11281 aagaaaggtt gctagctaca ttagttctat agaagcttgg caagaagatc cagggtggcg
    11341 catgattgcc cttcatctgg aaaagaaaga acatctggat taagctttct ctaaggaaaa
    11401 cttaagctag tttttaggtt tggctcttat actaaagtca tcaaaaagaa acgaggactc
    11461 tcatatgaca gtaacgatta aagtaaatta ccaaaccact ttccaaaaga aggaagcaaa
    11521 aaactagtat aaacagaaga gagagcgaaa tgctcttttt tcgtttctaa aactactttc
    11581 agcccatcat cctaaaagta aagaatctaa attcactttc tatttaccct tctttcttgc
    11641 attgattaca tagatatgct acagttgtgg taacgattac aaaataaaag gagcatgcta
    11701 tgaaaaatcc agctttgcta gaagaaatta agacctatag aggaagggat gaggttccgg
    11761 aagactttga tgctttctgg gatggggaag tgaaaaatgt ttccacactt ccatcctacc
    11821 acttggagga aagagatttc cacattcctc aagtcaagtg ctatgagtta acatttgaag
    11881 gaagcaagga aggaaaggtc tatgcacgca ttgttcttcc aaagagtgag gagaaggttc
    11941 cattaatctt ccattttcat ggttatatgg gacgtggctg ggactgggcc gacatgctgg
    12001 gcttcaccgt agctggttac ggtgttgttt ccatggatgt gcggggccag tcaggttact
    12061 cacaagacgg cttgcgttct cctttaggaa ataccgtgaa ggggcatatt atccgtggtg
    12121 ctatggaagg tcgggaccac ctcttttata aggatgttta tctggatatt taccagttgg
    12181 tcgaaattgt tgctagtctg tctcaggttg atgagaagcg tctttctagc tatggtgcct
    12241 cacaaggagg ggctctagct ctagttgcag cagcgctcaa tcctcgaatt cagaaaacag
    12301 ttgccattta tcccttcttg tcagacttca gacgggtgat tgagattggt aatactagcg
    12361 aggcttacga cgaacttttc cgttatttca agtttcacga ccccttccat gaaacagagg
    12421 aggaaatcat ggcgaccctt gcctatatcg atgtcaaaaa tcttgcccat cgtatccaag
    12481 gtgaggttaa gatgattacg ggcttggacg acgatgtttg ctatcccatt acccagtttg
    12541 cgatttataa tcgtctgacc tgcgataaaa cctatcgcat catgcctgag tatgctcacg
    12601 aagccatgaa tgtatttgtc aatgaccaag tctacaactg gctctgtgga agtgagattc
    12661 cttttaaata tctaaaataa ggagtcgact ctaagcacaa aatcttaaaa attacaaaca
    12721 cgcatagtat caggggatta aaaaaacttg atactatgcg ttttatcatg gaaatatagt
    12781 aaaatgaaac aagaatagga caaaacgatc aggacagtca aatcgatttc taacaatgtt
    12841 ttagaagcaa atgtgtacta ttctagtttc aatctattat atttatagaa ttttttgttg
    12901 ctagatttgt caaattgctt aaaataattt ttttcagaaa gcaaaagccg atacctatcg
    12961 agtagggtag ttcttgctat cgtcaggctt gtctgtaggt gttaatactt ttcaaaaatc
    13021 tctttaaacc acgtcagctt cgccttgccg tatatatgtt
//
LOCUS       DAVKFH010000048        12923 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKFH010000048 DAVKFH010000000
VERSION     DAVKFH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.048"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(553..2280)
                     /gene="ezrA"
                     /locus_tag="VW006_001556"
     CDS             complement(553..2280)
                     /gene="ezrA"
                     /locus_tag="VW006_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9380384.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2362..4308)
                     /gene="gyrB"
                     /locus_tag="VW006_001557"
     CDS             complement(2362..4308)
                     /gene="gyrB"
                     /locus_tag="VW006_001557"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9380385.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4323..4886)
                     /locus_tag="VW006_001558"
     CDS             complement(4323..4886)
                     /locus_tag="VW006_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9380386.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4971..5525)
                     /locus_tag="VW006_001559"
     CDS             complement(4971..5525)
                     /locus_tag="VW006_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9380387.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5536..6759)
                     /locus_tag="VW006_001560"
     CDS             complement(5536..6759)
                     /locus_tag="VW006_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9380388.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6796..9246)
                     /locus_tag="VW006_001561"
     CDS             complement(6796..9246)
                     /locus_tag="VW006_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9380389.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9446..10375)
                     /locus_tag="VW006_001562"
     CDS             complement(9446..10375)
                     /locus_tag="VW006_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9380390.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10515..10802
                     /locus_tag="VW006_001563"
     CDS             10515..10802
                     /locus_tag="VW006_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9380391.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10836..12170)
                     /gene="ciaH"
                     /locus_tag="VW006_001564"
     CDS             complement(10836..12170)
                     /gene="ciaH"
                     /locus_tag="VW006_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9380392.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12160..12834)
                     /gene="ciaR"
                     /locus_tag="VW006_001565"
     CDS             complement(12160..12834)
                     /gene="ciaR"
                     /locus_tag="VW006_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9380393.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3929 a         2750 c         2296 g         3948 t
ORIGIN      
        1 tctttagttg attttatatt actattatat aatgattttt tattttagtc tagtataaat
       61 agcgagaaat atctatccta tcttctagaa tgtcttccaa acgagaaaac tctcgtaaac
      121 aaagaggttt tagaggccta tttaccgtgg actaaagttg tacaagaaaa gtgcaaataa
      181 gaaatctcca gattaggaac tatccgtgag ttctctagtc tggagatttt tcaatagact
      241 tcgttattgg acggttacca agttactcaa agtacagatt tgaagctgca gataagactg
      301 acgaaatcac tttaatacta aatgaaaatc aaaaagcaaa ctaagaagct agcctcaggc
      361 tgtacttgag tacggtaagg cgacgctgac gtgatttgaa gagattttcg aagagtataa
      421 aacactgttt tgaggtggtg gataaatcgg ctgggtaaaa actcaatctc aggagaaaat
      481 gaagtaaatc tccccacaac tatagaaaaa gatttgattc tgctcctcac acaataaaat
      541 ctttttcttt tattaaaaac gaatcgtttc acgtgttttc tcatatgagg taacaaagcg
      601 attggttaca ccaggctctg ccacttccaa tgcttgagaa atcttgtcaa atgaagcgtg
      661 ataatcaaat tctttttcaa aaatatctaa agcttcgtta aatgcttctt gaatgcgttc
      721 atcaaatgag cgatagcggt tagaatattg caagagttgc tctgtcaaag ttgcatattg
      781 tacaatatta taagtttccg tttctaaagc ttccatatca ttcgttgcaa tttcaagaac
      841 tcgggtaaca gattcaatgt taatcatttt ttgttctaac tcaaccatta aatcctcggt
      901 attattgctt gccgtaaaga ataacttcaa gaaagtttgt ggaatacctg gcagattgcg
      961 tttttccatg tatcgcttga tagtatggag acgattgaca taaacattgg ccttttgacg
     1021 tgcattaata tcatctttct caatttgtgt caggcgctca ctaactgaaa tttgctcatc
     1081 ttcaatatct tttagttgag tttgtaaatc ctcaagattt tcttcaagaa ctgaataagc
     1141 ttgggttggt tcttcttgat ttgaagttac ctcaacaata gctgcctcaa aactctctaa
     1201 ttctgtctga atacgacgaa catggcttgc agctgtctca ggaagtaaat aggtcttgtt
     1261 caaacgtgca atatcttctc ccaataaagt attattctct ttcatatgtt gaaggtaagt
     1321 tggaagagtt gcaagtagat tttccactac tttctgagca gcaatttctc gagtaaaaat
     1381 atcatacaag gcattgattt cctcttgtgc ctgtccattc tcatattcgg cattatccaa
     1441 ttccaactga cgaatattct cttggttttt cttgaatgct tcataaagca agtggaaacg
     1501 cgcttcaata tccgtttcaa caaaatgata attagcatca attagtttac gataaccgga
     1561 ttccaaatcc tgtaattgat ctggcaattc tgtagaaagc gtcgtaacca aggctggaac
     1621 acgatccaca atatgactta aggccaaaat gtgattttct gtattatcca aaatcactgc
     1681 ggcttccaca gggtcacccg atgaattcaa ggttacaaat tgtgaaaatt cagattggat
     1741 attttctaat tgtttttcaa tttcatccaa ggcttgacca tactgttctg aattttcagc
     1801 aactctatgc tgaagttcct caaataaatc caaagcatga agaacacgac cactattttt
     1861 agattcttgc ttctctaagt ctgccaaagc attgcgaatt gccgcaatat cttcgtcaat
     1921 caaagtaatt tgactctcaa tttggtcaat ttgatgactg gccttgagaa aacgaaatga
     1981 atggttatag ccttctgctt caaagagatt attttcaata tcggcaaaag agttgagaga
     2041 taaatcgacc catttttgat tccattcacg gaaagccact tgactttgtc caatcaagtg
     2101 catatttttt acagcttcta cttcatcatt tactggaaga ttgtatagtt cttcttttct
     2161 ttcttctagc gcctctaatc tcccctcgtt tcgcttacgt agaaagattg ccactacata
     2221 agccagaact aaaatgactg caattgcaac cattaaataa attagttgtc cattagacat
     2281 atcaaactcc ttttttactt gaaacaatcg taacgattat atcatatttt ttagaccaag
     2341 ggaactactt ctccctattt tttagacatc aagtgtacta tagacagcat tttcttcgat
     2401 aaactcacga cgaggctcta ctcgatcccc catcaacata tcaaagattt tatctgcttc
     2461 tgcagcatca tctacagaaa ctctagccat caagcgatgt tcgggatcca tggttgtttc
     2521 ccacagctga tgatcgtcca tttcacctag ccccttataa cgctgaatag tcggtttggt
     2581 acgaccttca ctataacggg ctaaagcttc ttggagtttg atttcttgat ctgcacccgg
     2641 ctggatatat tctttaatct cgcttccaac cttgacacca tagattggtg gttgggcaat
     2701 ataaacataa ccagcttcta ggattggttt catataacga taaatcaagg ttaaaagaag
     2761 ggtacgaatg tgggctccat cgacatcggc atcggtcatc aaaacgagtt tttggtaacg
     2821 ggctttcgaa acatcaaatt ctgcgccaaa tcctgttccc atggctgtga aaagactacg
     2881 aatttcttcg ttagctagaa tcttatccat acttgctttt tcaacgttca aaatcttacc
     2941 gcgaattgga aggatagcct gaaactcacg gttacgacca gatttggctg atccaccagc
     3001 tgagtctcct tcgacgatga agagttctgt ttcagcaggg ttattagaag aacagtctgc
     3061 tagtttccct ggaaggttgg aaatttccaa accagatttt ttacgtgtga cttcacgcgc
     3121 acgcttggca gccacacgag ccttggcagc taaaatccct ttttccacga tacgtttggc
     3181 aatctgtgga ttttccatga ggaaatcgga gaaggcttca ctgaagaggc gattggtaat
     3241 cttgaccact tcgctatttc ccaatttggt cttggtttgt ccttcaaact gtggatttgg
     3301 gtgtttaact gagataactg cagttaatcc ttcgcgaaca tcttcccctg ttaaattatc
     3361 ttcattgtct ttcagtaact tattcttacg agcataatca ttgataacac gagtcagtgc
     3421 cgtacggaaa ccttgttcat gcgttccacc ttcatgggta tgaatattat tggcgaaact
     3481 catgacattt tcatggtaac ctgttgtgta ctgcatggct acctcaactg tgatatcatc
     3541 catctcaccg tctgtataga ttggtgtatc aaagattaca tccttgttct cgttgatata
     3601 ttcaacgtaa ctagcaatcc caccttcata atgataatgc ttggtttgtt ccaaaccttg
     3661 gcgcttatct gtaattgaaa tttgaagacc gcgatttaga aaggccaact cttgaatccg
     3721 tttatttaat ttatcaaaat caaagattgt tgtttcagtg aaaatttctg ggtccggtgt
     3781 gaagtgaacc gttgttccag ttctatctgt atccccaacc acctcaaggt ctgcaacaac
     3841 atgaccacga cggtattctt ggtaatgaat cttaccgttt ttatggacat gaacgtctaa
     3901 ttgagtggaa agggcattaa ctactgacga ccccaccccg tgaagaccac ctgaaacctt
     3961 gtatccacca ccgccgaact ttcctccagc atgaaggact gtaaagacgg tctcaacagc
     4021 aggacggcct gttttttcct gaatatcgac tgggatacca cgcccatcat ccacaacagt
     4081 aatcgaatca tctggctcaa taaaaacttg aatatggctg gcaaatcctg ccaaggcctc
     4141 gtcaattgag ttatcaacaa tttcccagac tagatggtga agaccttctt ttgaggttga
     4201 tccaatgtac atccctggac gcatacgaac agcctctaag ccctctaaaa cttgaatttg
     4261 actggcatca taatcctgtg cctgcagatt tttgatttct tctgtcatct tattcctttt
     4321 tcttatatgt ctaatacttg tcttaaattc acgatactgg taaacaagtg ggtatcaagt
     4381 cctgcagctt gacctgcttc gatatcaatc ggccgatcac caatgacaag accagagcta
     4441 atctgatact tttctcttaa ataaagcatg gattcgggat ttggctttct cttaaagcct
     4501 gagctagaag tcaccacttc tgtaaaataa gctgctatag aggttttttc taaaatttcc
     4561 aaaacctgat catttcgatg agagaccaaa aaatgacggc caccttgatt taaaatgtct
     4621 tccaataggt cagaaactcc ttcaaataaa atcgggtgtt caagctctct ggcttcattt
     4681 tccttgtact tttctaaaaa attctctaaa ttgggagcga atgtctcaat cgcaaaagga
     4741 gtagaaacct ttaaagcttg atagacactg tcatggtctt gtgtgatacc atacagtgcc
     4801 aatgtttcaa caaatgcagc tgttgaagtt tcataattat ccagtaaagt tccacctaaa
     4861 tcccagatgt aatcgtgata tttcataccc ttcattatag cattttttta tgaaaaaagc
     4921 gatgatttcc ctgagttttc cacataaaaa acgagagaat aactcccgtt ttactgattt
     4981 ttaccaaaga catctcgata gagcattcct gttcgtaaac tctctgcgtc ccctcctagt
     5041 tgctccacca actcgtaggc aaaggcaagg gctgttgaag gaccccgact ggttgtcaac
     5101 tgaccatcta ccactactgt ttccttgacg tagtgaccat caaggatttg ctcttgaacg
     5161 ccgtcataac aagtgtatcg cttatttttc aatatctctg cttgattgag ggcaattggt
     5221 gccgcacaaa tggctgctag tttcttccct tcttgctcga agctttgcaa ttcttgaatc
     5281 aaggtctgat tatcacgtaa atgtgcagaa ccaggcatac ctccaggaag aacaatcata
     5341 tcatagtctg ataaatctcc atcaaagaca tgatctgctc ttacttggat tgcatgcgaa
     5401 cccgttactt gctcttcaaa accaaccata tcacatgtga tattggctcg acgcaagaca
     5461 tctacaactg tcaaggcttc aatttcttca aagccctgag ctaacataac tgctactttt
     5521 accatgattt tctccttatt taatttgttt taatacaacc tttttccgtt tgaatgggac
     5581 ttttccgctc ttttcttcag ctaaattagt ctggtaactc atcgataaaa gcaaaccaac
     5641 accaatcaaa ttactgataa tagctgatcc cccttgcgaa atgaaaggca agggaatccc
     5701 cgtcaaagga agtagtccag tcacagcacc gatattctca aagatgtgga agagcaacat
     5761 cataatcaaa cctgtggaaa tataagtgta gaactggtta tttgatttaa gagtaatctt
     5821 caacatacgg taaatcaaca tgagatagag ggcaataacc aggacagagc caataaagcc
     5881 aaaatcttct gcaataaccg taaaaatcat atctgactct cgaactggga taagcagatt
     5941 cgaagcatta aatccctgac caaataagcc accactccca atggcaatct gcccttgagc
     6001 ctgctggtaa gtcgttgttt gggcaaactc aaagggattg agccaagcca aaatccgatt
     6061 aatttggtag gtcggcattc caatctggtg aagaaaagct cgtccgtcct tgctaataaa
     6121 gatagctaag aaaccagcaa ctcctgttac agcagtcaca aatactggga taataatttt
     6181 ccaagaaacc cctgataata aaacgattcc tgagaaaatg gctacaaaaa ccaaagccgt
     6241 ccccaagtca ctttgaagtg ctaaaagaac taggactgga atggtaaaga gaatcatcca
     6301 gaaaattaac aaaaagtcca gcggaaccgt gcgtctccat tccttatgtt tctttgtaaa
     6361 ttggacaatg acacgagcca acatgaggat ataggatatc ttcataaatt ctgacggttg
     6421 aaatagggta attccattta ttgataccca gtttttggca cccgttgatg caactaagct
     6481 tggattataa aatacaatcg gcaagatcat aagtcccaag cctaaaatat atagaaaggg
     6541 ggtcaccttc caaagaaatt ctgtattaaa gagcatgacc acaaaaccaa tcacaagccc
     6601 caaggcaatc caggcgacct gctgccctaa aatgggcaga atattattgg gataatcatg
     6661 actaacggct atatagatag ccaccacacc gatgaccagt agaaaaaata ctggcaagag
     6721 caaactataa tcgactctag agtcgagaga acgtttcata tgtactaacc ttatactttc
     6781 atacaatact atttatcaaa gttcattaaa aaatctatca atagcctcgt caacttcgga
     6841 tcgagagatg gttttaacag tcgcttcttc tgctagagat gctactattt gtttgccgta
     6901 tcgttttccg acgattctcc tatccaaaat aagagttaag gaacgttggt attcacgtct
     6961 catacttctt cccaaagcct gttttaaacg aataatggcc attggcaatt gataatcata
     7021 aaaggcattt ttcccttctt gattcagttc ttgattaatc tttttcgtca agggttcttg
     7081 aggattttgg aaaggaagcc tcggtacaac ttgaatcaca gaaggatggc ttgaaaaatc
     7141 aactccctcc cagaaacttg ctgcaccaag caagatttgt tgttcacctt tttcaaagcg
     7201 tttctttagc tgatgaacat ccccattttt atactgggcc aagtggctaa ctgtaagtaa
     7261 atccgatact gctagaagca tgtctttagc ggtaaagaga accaaaatcg gttgctggaa
     7321 agcttgaatt tccactagta aagcagctac ctctctggca tagacttcta aggaggtttc
     7381 tgttaccaga gggaaatctt tgaccataac cacttcttgt tcctgttttc cccgagattc
     7441 aatcttgaca aatttggctt caggatagcc taaaaggtct gccaaagaaa ccctctgact
     7501 aatctcaaga gtagccgata ctcccaagac ttggcaactc tcaggaagta aagaggacag
     7561 aagagtacgg cctgattttg tagaagaaat ctgaattttc ttttgactcg tttcagttac
     7621 ttcaagccag taatcacctt cagctgtaaa atagcgaacc agctcatcaa agccttccac
     7681 ttctaattct gaaaaatact gatggagatt gtccagagaa tctaagatat ttttcctaga
     7741 tttaccagac tgaaattgtt ctatcaagta gagacactca aagcgaatac tttctagtat
     7801 ccgttgttga accctgtttt cttctcctac taaagcctta tcaattaaat cgataataga
     7861 ttgtatatcg taggtctctt gaagcagatt ttctagagct aacaaaatct tttggacttc
     7921 atcaataatc agtaaacggt cactgacaaa ttcaggatta tcttcaagtc tggtcacgag
     7981 ataggcatga ttagtcacta aaagcttgca agtctctgcc ctttcttgac tacgtttcca
     8041 aaaatcttcc gtcacaaata agctctggga tgataaattc ccatcatgac gaaggtctgc
     8101 tagaaaatgt tggtaacggt agagttgccc gatttcatcc aaatctcctg tctctgtctc
     8161 agtaagccag accaagactt gcattttaaa gcgtctaaat aagcgatttt catcattttc
     8221 ctgcaaggaa tgataaaagg catccaactt cagataattt tgtggtccct ttaagctatg
     8281 aatatctgta tggaacactt ccttgagacg tttaccttct tcttccatga tttgattttg
     8341 aagaatcttt gtcggaacac taagaacaat ttgtcgctct ttggattgag ataaagcggg
     8401 taagagatag ccataggttt tcccaatccc tgtcggcgct tgaatcagag agaaaggttc
     8461 atctttcaat agcaagccaa cctctttagc aaaactttct tgttcctccc tcacttcaag
     8521 gttcaacaga gaaatatttt tagaaaagtc ttgagatagt tttcgtagct ccagagaagc
     8581 tgccgttttc ttaaaataca gaccttgaac ttggaccaag tctggagaac tcaggataga
     8641 ttggttgcga taagtttcct caataaccag gtaagactca tataggagag cgtcagccat
     8701 ttccagcaag cgttccaaga gacctttagg aagctgggtc atcttttccc gtaaaaaaag
     8761 aagtaattct gctgtagctt gggcatctga aagggctgtg tgtgcgtgtt taagaggaat
     8821 tcctaattct cgacacaaaa tcggcaagct atatttttcc agttcaggga aaaagacctg
     8881 ggccaattcg accgtatcaa cacgagggtt tcttagctca tagccttcaa aaaataaatt
     8941 ttccgccaag agattagcat caaactgaac attatgggct acaaaaatcc catcctccac
     9001 caagtcaaag atttttctgg caacttgcga aaaatcaggt gcttgcgcca gacgttggtc
     9061 tgtcaatcct gtcagttctt tgatatgagc atccaagggt tcatgtggat tgacatccgt
     9121 cgtatagtga tcgacgattt ctccatcctc aatcacgaca attcccactt ggataatttt
     9181 agccttacta cctgtgctag ttgcctctaa atccaccaca acatagcgat taccaatcgt
     9241 tttcattata aaaaattata ccaaaaaaag ggccatttgg cacctgcaaa cataggataa
     9301 ttttcaaact caagaacact acttttctta gaaatcccat cgaatagagc attctagcct
     9361 gctgattttt ttaaaatagt gaagaaatac gaaaaattga gagaagaaat gttttcatct
     9421 tccttctctc aacgaatcat gtattttatt taaccatgtc aggatcgtag tcataaaatc
     9481 ctgtatcaag gaaacggttt ttagggtgaa gtttacgaac ttcttcatcc agcaagaagg
     9541 cttggtcatg gctaaagttg ccctcttgaa tcaagctacg cgcttggata aagagttttg
     9601 caatctcaac aaagttctga ccaggagtgt agagcccttc tagtttccag cgagtaaagc
     9661 catgctccac caattctgtt aatttgatca tcaaatcaag gtcattgttg gcaaagatat
     9721 gggtcccatg attatcttca aaaatggaat agtggctctc tggatcactt ggctcagcca
     9781 agaagaggtc acgtttatgc gtcttttcat catcgatatg tgtaaagtta tagtagtttt
     9841 gcaagagtgg acgtttagaa tgatggatga cgctagcacc gtaaaccaaa acttcagcag
     9901 gaatttccaa aatctctggc attttgaaaa gttcagctga tggaatttca cgcgccaaaa
     9961 cagcctcaga tgcgccagcc ttttgtcccc agaagttaat ctgacgactg ctagttacca
    10021 tggttgaagc atcgtagatg gtcttaaatg aataaccatc gcggttaact acgtaaaaga
    10081 cgcctgcatc cccaatcgta atatagtctg tcttgatttc ttccaagaag tctaagaaag
    10141 gcttgatacg gtccatcata tcttggtgca tgagagcatt gaccgcaacg atcaattcct
    10201 taccagcatc atgaaccaac ttagcgattt cacgtaattg gtcataacta aaggtcgttg
    10261 gcagacgaag gccaaaatct ttctcaccga catagatacg gtctacgcca gcttcgagta
    10321 gttgttcaac ttgttcaata ctttcagcag ttgctgtaat gacaatcttt tccataagaa
    10381 aaattatacc acatttctcc aaaatcagcc atgctttgaa aatgaaaagg gactttattc
    10441 tttttgtaac ctttctgtaa taattctctg ccgaaaaaat gataaaatag gtatgtactg
    10501 ttaaggagag aatcatgccc gtaagaaaat tacaatccta tgaggtagac tatcaagaag
    10561 aattaaacca gcagcttcct cattaccaag cttatacacc tgaagcgcaa tctgatgcca
    10621 atctcaagga aatcctattt tttattaata tcgctgtttt ttgtatctgt attgctatct
    10681 ttagttttat ctttttagca ttaaaattat caactgctct tgcctttgcc gcagcaatcg
    10741 gattcagctt acttgtttta aaagttcaac ggtctattat caaacgaaaa cgtagaagat
    10801 aaatccaaaa tatcgcccca attggagcga tatttttatt ttttcttttt agatggtgtc
    10861 tgaatggcaa tcttcacttc aaagattgtt cccttgggtt tattatcttt gacagtaaca
    10921 gttcctttta gagcatctac aatttgcttg gctagggata atcctaaacc aaaaccacct
    10981 ttttgccggg ttctagcttt gtctactcga taaaaacggt caaaaatttt ctttttatct
    11041 tctgtcgaaa taccgattcc attatcagaa acaagtaaat aaagattgcg atcggtcgcc
    11101 gagataagaa aatcaatttc accatcctcc tcagtatact tgacggcatt atcgaaaaga
    11161 atggtcatca gttgtttcag aagaagctga tctgtgacaa ttgttcgatg gatacgattt
    11221 tcaaaacgga agacacgatt attttccgaa gcaatcatct catagtttgt gaaagttgta
    11281 ttaaaaaagc tagttggaac ttctgcaagc tccggcttaa tcccatcatc tctccgagct
    11341 aagttcagca agcttgtcgt taaaaaacgc atatttcgga cttcttccaa actcgatgca
    11401 atgctttcgc tcacatccat aatggtagct tctggcttac gaaaaagggt ctctaagcga
    11461 ttttgcaaaa ctgcgagtgg agttcgtaac tcatgactgg cattttccac aaaagactgt
    11521 tgcttctgca tactctcaag caggggccta acactgaccc tagctagata gagactggca
    11581 agtaaagaca aaatccagaa actagccatc acgaccacaa tcaattgctc atgcttttga
    11641 ctagcctgct ccaactgact ggtattaatc aagacagcag catacttgat attggttgaa
    11701 accgaactaa tattggtctc catcaaaatc acacgataga tttcttcctg cccatagcta
    11761 ttaaaaacct gaatctggta gatatgtcct agttctttct tttctaactt aatcttatcc
    11821 aagcccaaaa atcgatttcc agaaagaagt tgagtaaagt ctttatcaaa gagaatgact
    11881 tccgtattgg aactgacatt aggttttatt tctactttac tagcgtccgc cctagcattt
    11941 tctaaatctt taatctcttc tgttgcccta tttatagcca gctgaataac tgcttgagga
    12001 ttttcactca gtccatgaag cttatcgtcc accgaagtat aaagactcga atgcatgact
    12061 tgtaaaataa tcagagtcat tgtagaaaaa atcagggtga agacaccgaa gttgcggata
    12121 aaataactaa agtcatccgc ataccatgtt tttttaagtt tactgaacat cttttaaaag
    12181 atacccaaca ctacgcaagg tttgcaaatt ctctgcaaaa gtggttccct ttaatttctt
    12241 acggactttt gaaacataga cttcgacaac cgaaatcgtt gtatcactat caaatcccca
    12301 tagacggtca aaaatctgcg tcttaggcaa aatcacattt tgattttgaa ggaaataaac
    12361 tagtaaatcg aactctttcc ccagcaattc gacaggagta tcttcaactt taacggtatt
    12421 ggttgataaa ttaaccacga tattcccata agtcaaggtg ttttcattaa acttccctga
    12481 acgtttgaga agggcctgaa ttcgcatttt aagttcttct aggtagaaag gtttggtcag
    12541 ataatcatcc gctcccagtt caaatccatg tcccttgtca tccaaacttt ccttggcagt
    12601 cataatcaga actggtgtcg taattccctt ttcacgcaat tcttttaaga cttggaaacc
    12661 atttttttct ggcaacatca aatccagcaa aatcaagtca tagacaccac tctcagcttc
    12721 gtagagacct tcttctccat caaatacctg cataacatcc gcaaaatcgt ctaaaaagtc
    12781 aaatactgaa tttgacagac ctaggtcatc ctcaaccaat aagattttta tcatgagaaa
    12841 ctcctcctta ttaaaactat tataccaaat ttgccttaaa aaaaactcaa ctctctgcat
    12901 tttacatgag atagctgagt ttt
//
LOCUS       DAVKFH010000049        12858 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKFH010000049 DAVKFH010000000
VERSION     DAVKFH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.049"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(98..610)
                     /locus_tag="VW006_001566"
     CDS             complement(98..610)
                     /locus_tag="VW006_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9380394.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(635..1615)
                     /locus_tag="VW006_001567"
                     /pseudo
     CDS             complement(635..1615)
                     /locus_tag="VW006_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(2091..2900)
                     /locus_tag="VW006_001568"
     CDS             complement(2091..2900)
                     /locus_tag="VW006_001568"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9380395.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(2924..3457)
                     /locus_tag="VW006_001569"
                     /pseudo
     CDS             complement(2924..3457)
                     /locus_tag="VW006_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            complement(3459..4670)
                     /locus_tag="VW006_001570"
     CDS             complement(3459..4670)
                     /locus_tag="VW006_001570"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9380396.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="VW006_001571"
     CDS             complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="VW006_001571"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9380397.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(5665..6504)
                     /locus_tag="VW006_001572"
     CDS             complement(5665..6504)
                     /locus_tag="VW006_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9380398.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(6518..7339)
                     /locus_tag="VW006_001573"
     CDS             complement(6518..7339)
                     /locus_tag="VW006_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEU9380399.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(7476..8333)
                     /locus_tag="VW006_001574"
     CDS             complement(7476..8333)
                     /locus_tag="VW006_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9380400.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(8487..9221)
                     /locus_tag="VW006_001575"
     CDS             complement(8487..9221)
                     /locus_tag="VW006_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9380401.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(9221..9904)
                     /locus_tag="VW006_001576"
     CDS             complement(9221..9904)
                     /locus_tag="VW006_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9380402.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            10094..10324
                     /locus_tag="VW006_001577"
     CDS             10094..10324
                     /locus_tag="VW006_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9380403.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            10553..12811
                     /locus_tag="VW006_001578"
     CDS             10553..12811
                     /locus_tag="VW006_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9380404.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         3851 a         2905 c         2312 g         3790 t
ORIGIN      
        1 aattttgttc tttcacagag agctacctga gttctattca agctcagata gtactttctt
       61 ataaactaga caaactaact gtcattgtac catcagatta caagacatca tcgtcactca
      121 ccttggaatt caatgtcgta ccccaatggg taattttacg gtggggttga gctaaaattg
      181 gtctgttttc atagattgtt tgccatctat tccatagtag gcccgtcttt ttctcaatct
      241 taactcgcag atttctcata ttttctttga ttgggaagtt gaggacaaaa cctgcagtct
      301 ggttgcaacc gtttccttcc caagaatgac tacgaacaac ttggtttcca tctttatcta
      361 ctggaacttc ttcccaagtt atggagtagc gggcaatgta agctccactg tgttgaattg
      421 tcaatgtttt atctttcaca ggagtctgac tgattggttg aactggctta gaaacttgtg
      481 tcgccgtttc agcattcgta gctataaagc ggaaaacttc aacttctgca agactgaggg
      541 cattatagcc ttctagccca atccgaacat agcgcgcttt tttatggtta atcgtaattt
      601 gtgctgacac atctttagga gatattatac gttttcactc aatttcttta ccagaactgt
      661 ctaaaagaat gacatcaaag tttgccaatc tatcctgggc agtgtctgtt cggttgtaaa
      721 tattgatttg gcgaatggtt tcttctttag ccaaatctac ttgccaccaa ggcttagatt
      781 ggaagtttgt atgagtgaca gaattgtgac catagttacc atcgactttg ccatccacag
      841 ctcttctagc atctcctccg aaagctgtcg aactttgagt aacctgtttt cctaaggcaa
      901 tattttccat tggttcagcg cttggttgac tagaagattg aggtagagcc tgtttcttaa
      961 agaatcgcac tttatccaat ttcggatcac gataaccatg tttatccaag gtagcttgtt
     1021 taccaatatt aaagttagga atatcattca ttggactttc aaaatagcca cgagaacggt
     1081 gtttaaagcg tgaattacct gtaaatgtaa caatatcacg attattatga atcaagggag
     1141 tcacaacatt gtcattatca accacataat gcttaatttt tccactaaca gctaatttac
     1201 gactttccaa accaacatct cagaaaccat tcttagcatc ccaaacagtt ctggaagtaa
     1261 tcactttagg agcactgaaa gttgtcactt tcctcaatac atggttataa aaatcaggat
     1321 atttttggta atcttcaacc gctgcaatct gagctaggta gcctccaaga gaatgacctg
     1381 tcatatacaa cttagtaata cttatatcct tggctaattc cccaacaacc ttgcggatgt
     1441 catccgcttg agctggctct gttatctcta cttttgactc ttcccttttg ttttcaagaa
     1501 cttcacttgc ttccggaagc gtttgaggag ctagtcttgg ctcctctcta agaggagttt
     1561 ctagttttgg agcatcattt tctgactctt tcaattcctc cttagaatct agcacaccta
     1621 ttaccacttc agttgttaca tcttgcttta cttcattggc tgaaattcca gctggagcta
     1681 gaaatcaaag ccgactacaa cagatacaac tccgatactt aattttttat gccaaagcgc
     1741 ataattcgtt gaccaatttt cattatttct tatctttctt ttcgtttatt tacaagcaga
     1801 ctcaagcgaa atctcattgt gctatatatg ataagactga gaaattcaga aagtcaattc
     1861 attctcatct ctccttatca tactagtctg cttggctagg aagctaatgc tagtatccta
     1921 gaatcttgtc tttcacaatg ctggctatca gatcagacac acctattctt ccgcttcttt
     1981 cagttataag agcaggaaga agctcatagt attcctctgt ttcttcttta atacggatag
     2041 gcagtcaatc ctgaaaagac agaggcactc ccccttttaa atcttttttc ttacaattca
     2101 gcaagaatcg ctttaagcaa gcctttaaaa tcacctttaa cacgttcagt cacttctaca
     2161 acttcttcgt gattgagttc ttcttggaaa ccagccgcaa agttagtgat acatgaaatt
     2221 cccagaactt tcaagccaga gtgggctgcc acgataactt caggaaccgt agacatacca
     2281 actgcatctg ctcccagtgt cttataggaa cgaatttctg ctggtgtttc ataagtcgga
     2341 ccagtaactc cgatatagac accttcatca agcttgatat taagtttttt agccacttca
     2401 tgggcagtag cacggtattc tggtgtgtag gccctagaca tatctgggaa acgtgggcca
     2461 aagtcatcca agttttcacc catcaatgga ttttgccccg tcatgttgat atggtctgag
     2521 atagccatca aggtaccagg accaaatccg ataccgccag ctgcattggt tacaataaca
     2581 ccttcacatc caagaacttt catcacacga actgggaaag tcaccacttc cagaggattc
     2641 ccttcataga aatggaaacg cccttgaaga gccaagacct tgcgacctgc cagttcacca
     2701 tataccaatt taccagcatg accgactact gttgaacggc cccagtttgg aatctcagca
     2761 tagtctacta caactggatt ttcgatttct tctgccaatt ctccaagtcc tgacccaagg
     2821 attagaccga actcaggggc tgcaattccc ttttctttca ggaaagtagc agtttcatgg
     2881 attttgttta aaaatgtcat tgtgtgtctc ctttattatt tttttagcat ttgaccaatt
     2941 ttgtcaaagg ttttagcatt acgaatagta atgtggcggt agaaaggcac ctcagcaagt
     3001 acttatgata ggcagtctta gaataggatt cttcagaaaa tttcccctag aaaatcccaa
     3061 gttttccaaa ataaagcact tcatctttca gctctaaact ttcaactgtc gcgatgactt
     3121 ggtccacatc caaaccctca gtgtaaaaga gaaaatcttt tcgtgccaaa tctctgctcc
     3181 accaagctgg tagattttca agttccgcct caaagtcctc tagactcagt aaagaaaagc
     3241 tctgaataaa tggataatgg actgcaaaga aagtctctag cttttcaacc aattgggctt
     3301 tggaatctat cgaagtaaag aaaatattgc cactgttgat gtagctctca accttttcca
     3361 gtcccaagtt tgtcaattct tgacgaagct ccgccatgac gaccttattc ttaccaccaa
     3421 cattgatacc tctcaccagc aaagcatagc gcgtcatctt ataccaattt atctaagaaa
     3481 ctttccccaa tcatagcagt ttccacacca aagttatcgg caacagtcgc tgaaatatct
     3541 gcaaaatgtc ctactggaat gagaccattt cctttaaagg cagggctata ggccaacaat
     3601 ggaatatatt cccgagtgtg atccgttcct gcatacgttg ggtcatttcc atggtccgca
     3661 gtaatcaaga gaaggtcatt ctctctcata gctgcgataa tttcaggtaa gcgttcatca
     3721 aactcatgca agcaatcacg gtaaccgtga gcattacgac gatggccgta aagggcatca
     3781 aagtcaacta ggtttgtgaa tgagaatcct ttttcaaact cagcaagtcc catagtcttc
     3841 aatagtgtat caattccatg actatttgac ttgttgtgac ccatgtcatg gttgatacca
     3901 gcaccgttaa agatatcgtt gattttaccc acagcataag tatcgatacc agcctcattc
     3961 aatttatcca aaacagttgg ggaaaatgga gatacagcca agtcacgacg gtttgccgta
     4021 cgagtgaagt tacctggttc acctacataa gggcgagcaa tgatgcgacc aagaagggca
     4081 ggacgctcaa gggtaatcga acgagcgtat tcacagatac ggtacaattc atccaaagga
     4141 ataatgtctt cgtgggcagc aatctgcaaa acagggtcag ctgaagtata gataatcaac
     4201 tctccagttt ccatctgacg tggtccaaaa tcatcgataa cagccgttcc tgaataaggt
     4261 ttgttggctt cacgaataac cttgcgtcct gagaattctt cgatttttgt caggatttct
     4321 tctgggaatc cgttccagaa agtatcgaaa ggctcagtaa tgttgagtcc catgatttcc
     4381 cagtgtccag tcatagtatc cttaccaaga gatacttcct ctaattttgt tgcatatcca
     4441 gttggattgc tttcagctgc tacagtctta agaggagttt cacgaggaat atttccaaga
     4501 cctattttag ccatgtttgg gacattcaaa ccaactgttt ttgaaatgtg tcccagtgtg
     4561 tcagaagctc catctggaac ccctgcattg acaaagttat tagcatctgg tgctgcaccg
     4621 attcctacag aatccagtac caccaaatga atacgattaa atttagacat cttatgcccc
     4681 cttctatttt cctttttttg aagttgaaat ctgaactcca tcccgtccag caacgatgac
     4741 cttatccacc atttggttga ataaaccatg ctccacaaca ccaacgacat ggtccaattc
     4801 ttgtccaaaa gcaattggat tttcaatgac atccaaggcg aggtcaatga taaaattctg
     4861 catatccgtc acaaaacgtt ggccgtcttt ttcacggaaa cttggtttgt agccagctcg
     4921 ttcaaaacga cgaaagacct gttctgcacc atactgaacc acttctactg gcaatttaaa
     4981 agcacctagt ttttcgacca gcttgctttc atccaccacc caaatgtatt cttttgatgg
     5041 tgtcgcaaca accttctcca tcagaagagc accaccacca cctttgattc cattaaactg
     5101 actatccact tcatctgcgc catcaactgt cacatcgaca aagtctactt ggtcaataga
     5161 tttgagtggg atattaaggc cttctgcctg tttagtggtc acactagaag tcgttacagc
     5221 tataatctgc aatccttctt ccttgatacg acgaccgatt tcttcgacaa aataataggc
     5281 agtcgagcct gttccaagtc caacgaccat tccatccttg acaaactcag cagccttgat
     5341 acctgccatt ttcttcagat tttccactca aacacctcca ttaaagagct acctctatta
     5401 taccatgtaa aggcttttat ttcatctaaa agtaccttta gaatactgct tatttatcct
     5461 tgaaattaaa tttttttaat aaaagcagaa ctctgacaaa tcactttaag tacggatgta
     5521 aaccaaatca actctaacaa aaatagattt acaagagtaa aaaaacgaac aaatttagtt
     5581 tcttagcgag aagaatacta aacgttcgtt ttttgtgaat taagaataat gatacaaatt
     5641 atcaatattt tttgtctaac tattttagac atatcttttc attactttag gaagacaatt
     5701 actaatctcc gttggcaaaa ccacatactg ttcttgagct agttcttggg ctatggctga
     5761 atgaagatgg gtcgctactg ccacacgttc gtaaagacta gcttgcttaa actggcctgc
     5821 aaatcctgca atcatcccag caagcgtatc tcccattccc ccagtcgcct gatagggacc
     5881 gccaacctgt aactggtaat aatctgattg gccagcttgc caaatacgag tagctgaacc
     5941 tttctccacc aaaattgttc cttgagggaa ggaagttagg gcactagccg ttgcatcttc
     6001 cttttgcttt tcaatcgtaa ttccagataa tttttcccat tctttttggt gaggagttag
     6061 gataagctgg cttgaggaaa atgacaaact tgtcctagca agaatggtca aggcacctcc
     6121 gtctacaatc aaaatctgat tttgtcttaa gccagcaaag acctgtttaa ctagatcttc
     6181 tccaaaagca tcgtctcgta aacccggccc cagcaagaca acttctgcct tctccaattg
     6241 ctcttttaac aactgctggt cttgaagaga aaaggccata gcctcaggta aatggctgtg
     6301 cagggctgga atattttccc tatctgttcc aacggtcacc aatcctgcac cgctttttac
     6361 agctgccaaa gcagccatga tgatggcacc accataagga taagttccac caagaaacag
     6421 cagacggcca tagtctcctt tatgactagt acgagaacgt tcaataataa ctttttctag
     6481 taaggcttga tcaatcactt tcatcgttct tcctcaacta tcctttatct gattgtttta
     6541 aaacaacttg atagactttc ttagtccact cttctaagtc ttctccagca tagtcaagat
     6601 aatgcaccct atcttgtatt ttagttggaa taatattttc aaattggctc ttattatgag
     6661 ttggaataat acaaatatac aactcactaa ataaattcaa catgtcctta atttttgcaa
     6721 catggtagcc atcaaaaata taaccagtaa ctttaatcaa atctgtatag ataaaatgaa
     6781 aactcactcc aggaataaac ttgtcttgta attctttctc tgaaggagct cgacctaata
     6841 aacgctccat cgctaatcga taaccagaac ttgtatttga ccaccctaac atgacataat
     6901 caaaataatc tatcggatcc gatgcagcat ttctactatc caaaaccaac tcttcagcat
     6961 ctcgcctaaa ggctttcaca gcagataaga gctgacctgt tttaaagata gaaatcgctg
     7021 catctatagt cgtagtatga ctacagtagt ctgaagtcac taacctctta tgtagatcat
     7081 aagtcgaggc tactctttta tctgtagggg taaatggtgg cagaaaggga tgctgacaga
     7141 tgtaatctaa aatttctttt tttaaacaag aatcagcaca ctcaatactt gtttgacgtt
     7201 tatgaagttt ttcgtaagct ataaatttag caattttttc taattcccac tcttgaatat
     7261 ttggataatc aattagtgct aggtagtata ttccatccca ttgtaaacta tattccctat
     7321 tataaatttt taaaatcatc taatcacctc actccattat acaacaaaaa ggaggcgcag
     7381 acctcctttt gtaatcttat atctaaaatt taatattcat ttctgccatt ttaaatatag
     7441 ctatagaaaa tacactctct tcagcgattt ttctcttatt ttctatccaa tgtccgaagt
     7501 gctgcttgat aagtttgctc catcagcata gtaatggtca tagggccgac acctccaggg
     7561 actggcgtaa tatagctagc aagtggggca actgcctcat aatccacatc cccacagagc
     7621 ttaccatttt catcacgatt catcccaacg tcaatgacta ccgcacctgg tttgacaaag
     7681 tcagcagtca caaacttggc acgaccgatt gcgaccacca gaatatctgc tttagcagcc
     7741 accttagcaa gattatgggt gcgtgagtgg gtcaaagtca ctgttgcatt cttggccaaa
     7801 agaagctgag ccatgggttt tccaacgata ttagaacgac cgatgacaac cgcattttta
     7861 ccttctaatt caatcccata ttcatgaaac atttccataa ttcctgcagg tgttgatgga
     7921 atcatgactg gatgtccaga ccaaaggcgt cccatattta gaggatggaa accatccaca
     7981 tccttttctg ggtcaatagc caatagaacc gcctcttcat caatgtgttt tggtaatggc
     8041 aactgaacca aaatcccatg ccaagctgga tcctgattgt atttagcaat caggtctaac
     8101 aattcctctt gagtaatggt ctctggaact cgcactactt cgctacggga accagccgca
     8161 agagctgacc tctccttgtt gcgaacgtag acttggctgg ctggattatc cccaaccaaa
     8221 atcactacca aaccaggcac tagagctgtt tcttccttta attttgcagt cttttcagcc
     8281 aactgcccct gcaatttggc cgctaaagct ttcccatcaa taatctgtgt catatcactt
     8341 ctcttttctt tcaaatatct tccattatac caaaaactgc tagtgccctc taacacttct
     8401 ttcctaggtt atcctatagt ttcaaactat aagaaaaagc tctggttgaa cttttcacta
     8461 atcttgtctt gaaattttaa aatagattat aaaaccttac tcaagaaatc tttagtccgt
     8521 tgttcttggg tttgttcaaa aatctgctca ggtgtcccat cttcaacaac cacaccgtct
     8581 gccataaaga taacacgatc tgccacctca cgggcaaatc ccatctcatg tgttacgata
     8641 accatagtca tccctgactt ggcaaggtct tgcataacag ccaatacctc acctaccatt
     8701 tcaggatcca aggctgaagt tggttcgtca aagagcaaaa catctggttc catagccaac
     8761 ccacgcgcga tggcaatccg ttgttgctgc ccacccgaca gactctgtgg ataagcgtct
     8821 gccttatctg gcaaaccaac tttttccaaa agctcttggg ctctcttctc agcaacttcc
     8881 ctgctttcac ctttagtctt gataggtgac aaggtaatat tttccatcac agtcatatta
     8941 ggaaagaggt taaattgttg gaaaaccatg cccatcttct cacgcatggc aaacaggtca
     9001 ttcttcttgt ccgtaatatc gactccctca aagataagct tccccttggt tgcttcctcc
     9061 aacaaattca tagagcgaag caaggtagat ttcccactcc ctgaaggacc gatgataacg
     9121 acaacttctc ctcttttaat ctcgaggttg atgcccttca atacttcatt ctttccaaaa
     9181 gatttatgta aattttcaat ttttatcaag gtttctgtca ttatttctta tctccttgtc
     9241 ccatatgttt ttcaaaagct ttcaaggcta ctgtcagaat agaggtcata atcaagtagt
     9301 aaaatgctgc aaataaaagt ggtgttaaag gtagataggt tgttgtagaa actgttgtag
     9361 ccccattcca caactccatg accccaatag ctgataagag ggagctgtcc ttgataatgg
     9421 tgataaattc gttccccaat gctggcaaga tatttttgac tgcttgtggc aaaatcacat
     9481 aacgcatcgc atttttaaga cgaatcccta gcgaataagc cgcctctagc tgaccttttg
     9541 gaaccgcatt gattccggca cgaacagtct ccgaaacata agcaccacta ttcatagaga
     9601 taatcaaaat ccctggaatc agacgcgaaa aatcaacacc taaaattcca atctgaatag
     9661 tcggagcatt gatatgcata agagcaaagg caatcataat ttgaaccatc atcggtgtcc
     9721 cacggaaaat ccaaacgtac aagttggcca accaaacaag cggtttaaac tttgaacgtt
     9781 gcccaaaagc caagacaaca cccaaaatag ttcccaaaaa gataacacag atagaaataa
     9841 gaatcgtcac aacagcccca tagttaaaat aaggtaaata cttaggtaaa aaagaaaaat
     9901 tcatagtcac actgctttct aaaatagaat actgtatgat tataacatgt tttgaattaa
     9961 aatttaaatt ttttatccaa tttatttaaa aatctttaaa ctagcagaat tagcgagaaa
    10021 tcttagtttt ttctagtcaa gttgccctcc tttatggtaa aatagtaacg ataagaaatg
    10081 aaggagaatc aaaatggaat cacatttggt tagaatcatc aaccgccttg aagcaatggc
    10141 aaacgatggc ggaaatttaa aacgtaattt tgaacgcgaa ggagttgttg ttgcagaagt
    10201 tgcgtacagc cacgacgaag aaaacggccc aatctttacc cttcgtgacg tagaagatcg
    10261 cgaaacgtat acgtttgata gcattgactt aatcgcaatg gaaatttacg aacttctcta
    10321 ctaatctaaa acaagttggg attgtccctg catgtctaag taaaatcctt tttgtctcac
    10381 aaataggatt tttttatagt ccaaattcac aaaaattttc attgtaacaa cttctcaaag
    10441 cttcaatctt tttacttgct ttacacccca atcctgttat aatgagagta tagaatttga
    10501 ctatttttga cctttaaaca agtcaggcta aagaaagaaa tgaggtagat gtatgctttg
    10561 tcaaaactgt aaaattaacg actcaacaat tcatctttac accaatctca atggaaaaca
    10621 aaaacaaatt gacctctgtc aaaactgcta taagattatc aaaacagatc ctaacaatag
    10681 cctcttcaaa ggtatgacgg atctgaacaa tcgtgacttc gatccctttg gtgatttctt
    10741 caatgatcta aacaatttca gaccttctag caatactcct cctattcccc caacccaatc
    10801 aggtggaggt tacggtggaa acggcggtta tggttcccaa aatcgtggat ctgctcaaac
    10861 tccgccacct agccaagaaa aaggcctgct ggaagaattt ggtattaatg taactgaaat
    10921 tgcccgtcgt ggagacattg accccgttat tgggcgcgac gatgagatta tccgtgtcat
    10981 cgagattctc aatcgtagaa ccaagaataa tcctgtcctt atcggtgaac ctggtgtcgg
    11041 aaaaacggcc gttgtcgaag gtctagctca gaaaattgtc gatggcgatg tgccacataa
    11101 actccaaggt aaacaagtca tccgtctgga tgtggttagc ttagttcaag gaacggggat
    11161 tcgaggacaa tttgaagaac gcatgcaaaa actcatggaa gaaattcgca aacgtgaaga
    11221 catcatcctc tttatcgatg aaatccatga aattgttggt gctggttctg cgagtgatgg
    11281 taatatggac gcaggaaata tcctcaagcc agcccttgct cgtggagaac tgcaactagt
    11341 cggtgctact accctcaatg aataccgtat cattgaaaag gatgctgccc tcgagcgtcg
    11401 tatgcagcct gttaaagtcg atgaaccaac ggtggatgaa acaatcacta ttctcaaagg
    11461 gattcaaaag aaatacgaag attaccacca cgttcaatat accgatgctg cgattgaagc
    11521 agctgcaact ctttccaatc gctacatcca agatcgcttc ttgcctgaca aggccattga
    11581 cctcctagat gaagctggtt ctaagatgaa cttgaccttg aattttgtgg atcctaaagt
    11641 aattgatcag cgcttgattg aggctgaaaa tctcaagtct caagctacac gagaagaaga
    11701 ttttgagaag gcggcctact tccgcgacca gattgccaag tataaggaaa tgcaaaagaa
    11761 aaagatcaca gaccaggata ctcctatcat cagcgagaaa actattgagc acattatcga
    11821 gcagaaaacc aatatccctg ttggtgattt gaaagagaaa gaacaatctc aactcatcca
    11881 tctagccgaa gatctcaagt ctcatgttat tggccaagat gatgcagtcg ataagattgc
    11941 caaggctatt cgccgtaatc gtgtcggact tggtacccct aaccgcccaa tcggaagctt
    12001 cctcttcgtt ggaccaactg gtgtcggtaa gacagaactt tccaaacaac tggctatcga
    12061 actttttggt tctgctgata gtatgattcg ctttgatatg agtgaataca tggaaaaaca
    12121 tagtgtggct aagttggtcg gcgctcctcc aggttatgtt ggctatgatg aggctggtca
    12181 attaactgaa aaagttcgcc acaatccgta ttctctcatc cttctcgatg aagtggaaaa
    12241 agctcaccca gatgttatgc acatgtttct tcaagtcttg gacgatggtc gtttgacaga
    12301 cgggcaagga cgcaccgtta gcttcaagga tgccatcatt atcatgacct caaatgcagg
    12361 tacaggaaag accgaagcta gcgttggatt tggtgctgct agagaaggac gtaccaattc
    12421 tgtcctcggt gaactcggta acttctttag cccagagttt atgaaccgtt ttgatggcat
    12481 tatcgaattt aaggctctca gcaaggataa cctccttcag attgtcgagc tcatgctagc
    12541 agatgttaac aagcgcctct ctagcaacaa cattcgtttg gatgtaactg acaaggtcaa
    12601 ggaaaagttg gttgacctag gttatgatcc aaaaatggga gcacgcccac ttcgtcggac
    12661 tattcaagac tatattgagg acacaatcac tgactactac cttgaaaatc caagcgaaaa
    12721 agatctcaaa gcagttatga ctagcaaggg aaacattcag attaaatctg ccaaaaaagc
    12781 tgaagttaaa agttctgaaa aagaaaaata aatcctatag aaaaggagta gaaaatgaaa
    12841 tttttctgct tctttttt
//
LOCUS       DAVKFH010000050        12794 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKFH010000050 DAVKFH010000000
VERSION     DAVKFH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.050"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            122..277
                     /locus_tag="VW006_001579"
                     /pseudo
     CDS             122..277
                     /locus_tag="VW006_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VW006_001580"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VW006_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9380405.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VW006_001581"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VW006_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9380406.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKANFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7569..8747
                     /locus_tag="VW006_001582"
     CDS             7569..8747
                     /locus_tag="VW006_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380407.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            8956..>12794
                     /locus_tag="VW006_001583"
     CDS             8956..>12794
                     /locus_tag="VW006_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9380408.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEPSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPS
                     AEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHN
                     FINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVI
                     KLPETTGREHVELVKNYSKVNLNDKLNVHGYPARKYTHLKDATVEMEQEYANNTYGVQ
                     YQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVY
                     DELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENY
                     FMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKT
                     QQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGT
                     NNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHA
                     DGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLID
                     KSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLW
                     DPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNSIKANGTYSNKNNKVVNVVQEDDEI
                     LTKDSTVQRGEVLEDAQ"
BASE COUNT         4238 a         2441 c         2695 g         3420 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct accagtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cggacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 atcatgagat gaccaaatcc cagatgagtt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaaggtc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagtat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc
     1681 agtctgatat tgatgctttt gagcaatata tccgctatct tggtatcaat ggcttgccag
     1741 cctttcagca aatcttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttctg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggcaaaacc taaaccaagc gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggaa gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaaattgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg cagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggtcgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc ggcagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtacttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgtgctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag atagcgattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagagaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatccccaa gaaggcaagc tcattctcct caaggcaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac aagcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg aattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc tacaacctca aaacagtgtt
     7501 ttgagctgat tgcggctagc tttctagttt gctctttgat ttttattgag tattagaaag
     7561 gagacctcat gacacttcca gttagaaaat ccctgcacga tgcagtttta caggcttcga
     7621 aagcggatac ttgggaacaa gctaccaagg aatggaatga agtttccttg atttttaacg
     7681 gtatcggccg tagcaattgt gtctgtggaa atgccatcaa atacgcttat gaactcttta
     7741 acggtgtcac aggccaacgc ctctttccaa ttggtagcga ctgtgttcgt cattttcatc
     7801 gattgtccct tgaccagcaa ttggaagagg aagaaaaact gctcagaaag gttgaaaatc
     7861 taaccagaaa agctcagaaa aaggaaaaaa tcaaggtcaa taaaagtgac tttgacgagc
     7921 gacttctaaa atggctctgg gaaaaaggtg ttttcaaacc caatcgtggc aatcaatttg
     7981 cgcctgagag agactaccag cttttcctag aagtctttca gggaggaagt tggaccaagg
     8041 cggaaccaaa gaaaaaggct cggatggaag aagtccttga aaagtgtatc aaaccctttt
     8101 tacttgggaa atcggatgac caactctacc ttgtcaagct aggcaaggag aaaattgact
     8161 acgaacaaga actccgtatc caggcagaga aagaacgcaa gaagagggat aaaatcgcca
     8221 agcaatatgc agacaatctg gttctagcca tgggacccgc agagcgtgct tatcaagatt
     8281 actttggctt tacagagacc ctgactcaag aagaacgcaa gtgggaaaaa attctttttg
     8341 gtaaaaatag agcagaacgg gctatcaagg ccaaacaata ccaaaaagag ctggaaaaag
     8401 accaacgaat tgccagtcag gatccaattg aaagaaaaca gaagcagact tggctcctca
     8461 attcctattt tcgtgagctt cctgaagaaa aagctcgatt ttctaggctc ttattagaat
     8521 atcgaaaaag tggtgaagta cccttttcaa ctgaatacct gtccgatcat ctcatcgact
     8581 ttttctataa aatgaaagcc tttgaatttg aaatcgcacc agaacaagtc cgagattttc
     8641 taaaaaaaag cctgcaggaa gaccatcgct catcagcaca aggaagctgg atagaaggaa
     8701 ttctcctcaa ctgcctcaaa ccatttttag aacgattagt gatatgagta acacctaccg
     8761 tgtaaatttc atggtaggtg ttattgtacc cccaaataat ataaaacaaa gtatgaaagc
     8821 gcatctcaat tttaagactt ttttgtaaat taagagtata attaaggtat actgcctttt
     8881 ctatagataa tagataatac actaatataa taaaggtctt tgtaacatat ataaatttta
     8941 tttcaaggag gaataatgga aaagtatttt ggtgaaaaac aagagcgttt ttcatttaga
     9001 aaattatcag taggacttgt atctgcaacg atttcaagtt tattttttat gtctgtatta
     9061 gctagttcat ctgtggatgc tcaagaaact gcgggagttc actataaata tgtggcagat
     9121 tcagagctat catcagaaga aaagaagcag cttgtctatg atattccgac atacgtggag
     9181 aatgatgatg aaacttatta tcttgtttat aagttaaatt ctcaaaatca actggcggaa
     9241 ttaccaaata ctggaagcaa gaatgagagg caagccctag ttgctggtgc tagcttagct
     9301 gctctgggaa ttttaatttt tgctgtttcc aagaaaaagg ttaagaataa aacggtatta
     9361 catttagtat tggttgcggg aatgggaaat ggtgtcttag tttcagtcca tgctttagaa
     9421 aatcatcttt tgctaaatta caatacggac tatgaattga cctctggaga aaaattacct
     9481 cttcctaaag agatttcagg ttacacttat attggatata tcaaagaggg aaaaacgact
     9541 tctgattttg aagtaagtaa tcaagaaaaa tcagcagcca ctcctacaaa acaacaaaag
     9601 gtggattata atgttacacc aaattttgta gaccatccat caacagtaca agctattcag
     9661 gaacaaacac ctgtttcttc aactaagccg acagaagttc aagtagttga aaaacctttc
     9721 tctactgaat taatcaatcc aagaaaagaa gagaaacaat cttcagattc tcaagaacaa
     9781 ttagccgaac ataagaatct agaaacgaag aaagaggaga agatttctcc aaaggaaaag
     9841 actggggtaa atacattaaa tccacaggat gaagtgctat caggtcagtt gaacaaaccg
     9901 gaactcttat atcgtgacga aactatagaa acaaaaatag attttcaaga agaaattcaa
     9961 gaaaatcctg atttagcaga aggaactgta agagtaaaac aagaaggtgc attaggtaag
    10021 aaagttgaaa tcgtcagaat attttctgta aacaatgaag aagtttcacg agaaattatt
    10081 tcaacttcaa cgactgcgcc tgtttcaaga atagtcgaaa aaggtactaa aaaagctcaa
    10141 gttataaagg aacaagctga gactggtgca gaacataagg aagtacagtc tggagctatt
    10201 gttgaacccg caattcagcc tgaactacca gcagctgtat taaccgacaa aggtgagtca
    10261 gcagtccaac cagagttgcc agaagctgta gtaagtgata aaggcgtacc agaagttcaa
    10321 ccagcgttac cagaggcagt catgactgac aagggtgatc cagagcaggt agaaccgcta
    10381 ccagaatata caggagtaca agctggagct attgttgaac ctgaaaaagt tgaaccggaa
    10441 tatgctggag tacaagctgg cgctattgtt gaaccagagc aggtagcacc actaccagaa
    10501 tacacagggg tgcaagccgg agcgatagtc gagccagaga aagtagaagc accgaaggaa
    10561 tacacaggag tgcaggctgg agcgatagtc gaaccagaga aggtagaagc accgaaggaa
    10621 tacacaggag tgcaagccgg agcaatagta gaaccagaaa aagtagaacc atcaaaagaa
    10681 tacacagggg tgcaggctgg cgctattgtt gaaccagagc agatagcacc actaccagaa
    10741 tacacagggg tgcaagccgg agcgatagtc gagccagaga aagtagaagc actgaaagaa
    10801 tatactggta aaatcgaaca acctagtgca gaagatacta aaccaaataa tgaaaatact
    10861 aatacaccag aagaaatgag tattcagaaa aaatctagtg cattaattaa tatgaacttt
    10921 attacagatt cgtcgaaggt tactggagta ggatcagcta cttttattgc accaaatgta
    10981 cttttaacag tagcacataa tttcattaat aattctactg ataatactac tggcgaattt
    11041 agaggtgata aatctaaaaa cgtatatgag tgggtgactc ctgatggaca aaaaggaaca
    11101 tttactgcta acaatattca tttttataat aaaaaagatt atccaaaagg atttatctat
    11161 gatttagcag tgattaagct acctgaaaca actggcagag aacatgtaga attagttaaa
    11221 aattattcga aagtaaatct taatgacaaa ctaaatgttc atggatatcc agctaggaaa
    11281 tatacgcact taaaagatgc tacggtagaa atggaacaag aatatgcgaa taacacctac
    11341 ggtgttcaat atcaaggtgg aaatcctggt atgagtggtg gaggaatatt taatgctaat
    11401 ggtgaagtta ttggtgtgca tcaaaatgga gcacaaaatc gctcaggcgg attgatttta
    11461 tctcctacac aattagcttg gattaagagt attatagcag gtaatgaaat tccaccagta
    11521 tacgatgaac tttatcgtca taaagatgag aaaaaagatg acgctaaaga tgaaaaagaa
    11581 gttattaaga aattagaact tagaaatata tcttctgtag aactatattc aaaagatggt
    11641 aataagtatc gacatgtaac ttcacttgct tctttaccaa gtaatgcaga aaattacttt
    11701 atgaaagtta aatcagagaa ctttaaagat gtgatgctac cagttacaag cattactaat
    11761 gatactaaag ataatagaga tgtttataag atagtagcaa gtgcaaatag tctgatacaa
    11821 catgaaaata ataatgtact agaaaattat acatactatt taccaaaaac tcagcaaagt
    11881 gaaactgggg tatatacatc atttaaaaat ttagtagatg caatgaatag caatcctaat
    11941 ggcacattcc gtttaggagc aactatggat gctcgtgaag ttgaacttcc agatggtcaa
    12001 gaaagttatg tgaataatgt attccatgga atattagtag gaacaaataa tgagaaatat
    12061 tatgctattt ataatttgaa aaaaccatta tttggggaat taaatggtgc tacagtagaa
    12121 aaactttcac taaaagatgt taatatttcc gcaaaggacg atactgcaac acttgcgaaa
    12181 gaagcaaata acaatactca tattgataac gttcatgctg atggagcgat agctggggaa
    12241 cgtagcatag gaggattagt atcacaagtt aataattcta ctatttctaa cagtagctat
    12301 acaggtagaa taactaatac atataaaaca gttgctagtt atcaaatcgg aggtttagta
    12361 ggcaaacttt ctggtccgag gggattaata gataagtcgt tcgcatcaat cgatttatct
    12421 tcaaatgcaa cacaaggaga ccagtctatt ggtggaatag ttggtgcagt agaaaatagt
    12481 gcgcttatta gtaatagtta tgctgaagga aatctaaata atgttcaacg ttttgcaaac
    12541 gttggtggag tagttggtaa tctttgggat ccggttggag gattagaaaa atccggtcga
    12601 ctttctaatg tactaagcga tgttaatgta acgaatggta atgctattgc aggttataat
    12661 tttaatagta taaaagcaaa tggcacatac agtaataaaa ataataaggt tgtgaacgta
    12721 gtacaagaag atgatgaaat cttaactaag gattctactg ttcaaagagg agaagtatta
    12781 gaagatgcac aaat
//
LOCUS       DAVKFH010000051        12765 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKFH010000051 DAVKFH010000000
VERSION     DAVKFH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.051"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..319)
                     /locus_tag="VW006_001584"
     CDS             complement(<1..319)
                     /locus_tag="VW006_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9380409.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKI"
     gene            562..855
                     /locus_tag="VW006_001585"
                     /pseudo
     CDS             562..855
                     /locus_tag="VW006_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            962..1313
                     /locus_tag="VW006_001586"
                     /pseudo
     CDS             962..1313
                     /locus_tag="VW006_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1589..2895
                     /locus_tag="VW006_001587"
                     /pseudo
     CDS             1589..2895
                     /locus_tag="VW006_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2935..3945)
                     /gene="tsaD"
                     /locus_tag="VW006_001588"
     CDS             complement(2935..3945)
                     /gene="tsaD"
                     /locus_tag="VW006_001588"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9380410.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3935..4372)
                     /gene="rimI"
                     /locus_tag="VW006_001589"
     CDS             complement(3935..4372)
                     /gene="rimI"
                     /locus_tag="VW006_001589"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9380411.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYVLAYDGAEVIGFLAVQENIFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4369..5052)
                     /gene="tsaB"
                     /locus_tag="VW006_001590"
     CDS             complement(4369..5052)
                     /gene="tsaB"
                     /locus_tag="VW006_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9380412.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5230..5415
                     /gene="cibA"
                     /locus_tag="VW006_001591"
     CDS             5230..5415
                     /gene="cibA"
                     /locus_tag="VW006_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9380413.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5417..5569
                     /gene="cibB"
                     /locus_tag="VW006_001592"
     CDS             5417..5569
                     /gene="cibB"
                     /locus_tag="VW006_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9380414.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5892..6107)
                     /locus_tag="VW006_001593"
     CDS             complement(5892..6107)
                     /locus_tag="VW006_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380415.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            6387..6620
                     /locus_tag="VW006_001594"
     CDS             6387..6620
                     /locus_tag="VW006_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9380416.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6622..8301
                     /locus_tag="VW006_001595"
     CDS             6622..8301
                     /locus_tag="VW006_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9380417.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            8355..8447
                     /locus_tag="VW006_001596"
                     /pseudo
     CDS             8355..8447
                     /locus_tag="VW006_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8654..10567)
                     /gene="mnmG"
                     /locus_tag="VW006_001597"
     CDS             complement(8654..10567)
                     /gene="mnmG"
                     /locus_tag="VW006_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9380418.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10577..11032)
                     /locus_tag="VW006_001598"
     CDS             complement(10577..11032)
                     /locus_tag="VW006_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9380419.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQYGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(11173..12294)
                     /gene="mnmA"
                     /locus_tag="VW006_001599"
     CDS             complement(11173..12294)
                     /gene="mnmA"
                     /locus_tag="VW006_001599"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9380420.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3651 a         2957 c         2532 g         3625 t
ORIGIN      
        1 ggatttttaa taattttaaa atatttttaa cgtgtaaggc agtttcaaaa ttatttgaac
       61 attgagtaca atcaacatag gcaatatcta aatttttata atcatcaata acctttgaat
      121 ctctagatac gattatcaaa ataggatata gagacatcct tctgatctgc tttttacaaa
      181 gtccaattat atgcggatct atacctccac aatgtccatt attataccta actataatat
      241 gagccgaaaa cactatatcc ttaatgtctc catatccatc agggatatta atatttattt
      301 ttccacaact atattgcatt gtaaccatct ccttaaacga cgcattatga tatttgatag
      361 agaaattttt atgaataact caataatttt atagtaaatc atgcttatat ctcaaagata
      421 cctattttct cttgtctcga ccttctccaa agaattgcta taatactatt acaaatacat
      481 ctgcactaca cttcaaattt tagcactgta taaaaacgtt tcaacacact aactttaaga
      541 aaacttccac tattaattga aaaagttgat agagataaat taaagatcta tattgaaact
      601 catcccgatg cttatttgac tgaaatagct gctgaattca actgtcctcc aacaactatt
      661 cattacgctc taaaggctat gggatatagt ctaaaaaaga gctgtaccta ctgcgaacaa
      721 gacccagaaa aagtaaatcg gttccttaaa gaattgaatc acttaagcta cctgactcct
      781 atttatattt atgagacagg ggttgagacc tatttttatc tcgaatatga tcgagccttg
      841 agcaggcagt tagtctctct ggaagaagat ataattattt gaattaagat cgagacaacg
      901 cacaccagag attgcgatac tgttatagaa gtactaatgc ccttttttgt ttcaatatac
      961 tatggctccg atgacctata aagatacgat gacgagtgac tttttcgaag cttgcttcca
     1021 aaaattctta ctacctactt tagatacacc atcccttatc attatggaca atgcaaggtt
     1081 tcacagaatg aacatgtgta aggagcaggg catagactgt taccacttcc tacctattca
     1141 cccgagtata atcccattga gaaaatatgg gcttacatca aaaacatctc agaataatat
     1201 tgtcaaatta cgatgctttt cttgaggcac ttttgtccta ttcttgtttc agccgactat
     1261 actccgttat tgggcagcta cggaacagtc gatgggacga tggggggaca taaaaaaatc
     1321 ctccagtttt gttttttata acagtatact ggagaattga caatctcggt agatacctcg
     1381 ttatagcgcg gttacttatt aggcagttac aaaacaactg tgaacagaaa acattccaga
     1441 gtcagacaag actttggaat gttttggctc tataatttct gtagtgggta atcccacccc
     1501 aggaattata gggtcgtttc ttgtagaaaa aaagccccat atgacctata atgaaaagcg
     1561 tctaaccaac tcattagaaa gggttcatat ggaacaactt aagaatacca cagatttgct
     1621 cggattgaaa gacaaaaata tcaaaatctt gtctgttctg aaataccaaa cccatctagt
     1681 cgttcaggca aagttggatt cccccgctcc tccttgtcct cattgtcaag ggaagatgat
     1741 caaatacgac ttccagaaag cctctaaaat tccgcttctc gactgtcagg gtttacccac
     1801 ggtactacat ctcaaaaagc gccgctttca gtgcaagaat tgccttaagg tggtcgtttc
     1861 tcaaacatcc attgtcaaga aaaattgcca gatttccaac atggtgagac aaaaaatcgc
     1921 tcagctcctc cttgaaaagc agtctatgac tgagattgcc cacagattgg cggtctcaac
     1981 ttccaccgtc atccgaaaac tgagggaatt taagtttgaa accaattgga ccaagttgcc
     2041 aaaagttatg agttgggatg agtatagctt caaaaagagc aaaatgagct tcattgccca
     2101 agattttgag tccaaatcca tcctcgcaat tttagacggg cgaactcatg cggtgattcg
     2161 aaaccatttc caacgctatc agagagaggt tcgggagctg gtcgaggtca tcaccatgga
     2221 catgtacagc ccttattatc ggctcgctaa gcaactcttt ccaaaggcga agattgttct
     2281 tgaccgcttc cacattgtcc aacatctgag ccgagctatg aaccgagtac gaatccaaat
     2341 catgaaccaa tttgaccgaa aatccttgga gtatcgggcg ctcaagcgct tttggaaccc
     2401 tcgctttttc gtttctaggc tcgggctaaa tcagtccact ggactgattt actacaccag
     2461 gatagcttca agctctgtca gaaacgattc tatcagccca cgtttcgaat gcacttaacc
     2521 catcgggaag tacgagataa gctgctttct tactctgagg gattacaggt tcactacgaa
     2581 ctctatcaac tcctgctctt tcattttcaa gagaagaatg ccgaccattt ctttggattg
     2641 attgagcaag aactgccaac ggttcatccg ctttttcaaa cggtcttttg gactttttta
     2701 agggatagag ataagattat caacgcactt aagctgcctt attccaacgc taaacttgaa
     2761 gcgaccaata atttgattaa ggttatcaag cgcaaagcct ttggtttccg gaactttaac
     2821 aattttaaaa aacggatttt gatgactttg aacatcaaaa aagagagtac gaatttcgta
     2881 ctctccagat tgtagctttt cgcctaccca ctacacttga caaagagcca ctctttattc
     2941 catggtatca aaggcaagac ttggtttggc attgaggtcc cagcctgcga agttttcttt
     3001 gttccactcg ctgacgctgg cataggcaat catacctgca ttgtctccgc agagtcgcag
     3061 aggggggatg ataaccttga catctgtgat ttcggctgct aggcgttctc tgagaccttt
     3121 attggctgcc acaccacctg ccacaactag ggttttaaca ggatatttct ccaaagcctt
     3181 cttggttttt gccatgagaa tgtccataac tgctgcttgg aaggaagcac acaaatcttc
     3241 tgtagacagg ctttctccct tttgctcggc attgtgatga agattgataa aggcagattt
     3301 caaacctgag aaggagaact ccagattatc ttccttaatc atggcacggg ggaaatcata
     3361 aatatcctgc ccctgatgag ccagctcgtc aatctcacga cctgcaggat aggtcaagcc
     3421 catgacacga ccgaccttgt cataagcctc cccaactgca tcgtctcgtg tctccccaac
     3481 aatcttgtaa tctcctgcct ccgaaacata gaccaactct gtgtgcccac cgctaaccaa
     3541 gagggctagc aagggaaact ccaaaggctc cacactctga gctgccatga ggtgcccagc
     3601 catgtgatta acaggaatca gtggaagtcc gtgagcccaa gcaaaggcct tggcagctga
     3661 caaaccaact agcaaggctc cgaccaagcc tggtccgtag gtaaccgcaa cagctgtcac
     3721 gtcctcttcg gtaatccctg cttctgccaa tgcctcctcg atacaggctg taatgacctc
     3781 gacatggtga cgactggcta cttcgggcac tacgccacca aaacgtttgt gactctcaat
     3841 ttgactagca atgacattgg acaagagctc atcgtcgttt ttcaagacgg cgacactggt
     3901 ctcatcacag gatgtctcaa atgctaaaat atatctatcc ttcatctatt tctctcttca
     3961 tgataatggc gtcctcgact gggtcatggt agtaggcctt tcgctcagcg atgaccgcca
     4021 ttttttcttt cttgtaaaat gcttgcgctc gttgatttga ctttctgact tcgaggaaaa
     4081 tctccttatc tgtcggcaat tgagcaaaca aggctgacgc aattccctga ccctgataag
     4141 ctcctttaac agcgatttgc aagacttctg cctcaaagat attttcctga acagctagaa
     4201 aaccaatcac ttctgcccca tcataagcca gaacatacca agtctggtct tgggacaggt
     4261 ctgcttggat ttgctccaga gtccaaggac tgactaggta aacagctgcc ataacagcgt
     4321 agatggcttg agctaggtca ggctgttgtt gaattcgctt gatttctatc ataggcgttt
     4381 aatgtaggac tcaccagact cggtatggtt cttgagccag ttttcctcag cctcgactcg
     4441 tttgaggtaa ttcggcacaa aatcatgcaa ggagtctgct tccttgtccc aagctaaaag
     4501 agcaagattg gctgcattgg gcaatgtttc tttgtaatca gtccttggca agtgtttttg
     4561 aatctgctca acaaaggggc caacttctcc gacaaaggtt acctgactag tacccttgac
     4621 tttttctagc acctcttcaa aagataggtg cgcttctgcc atgacaggtt tggcattttc
     4681 ataaaatcct gcataaacat tattgcgacg cgcatccatc aaggggacaa acaaaccttc
     4741 ttgttggtgg ggcaccagag ccaagagact cgacatacca accaactcga tgttcagggt
     4801 gtaagctaag gtcttagcag ttgctaccgc aattcgcaag cctgtatagc tacccggccc
     4861 ttcagctacc acgattcggt ccaaatcctt gggtgtccaa tccaaacttg ccatcaaaaa
     4921 atcgatggca ggcataagag taatactgtg atttttctta atattaatcg tcgtctcggc
     4981 aagaacctgc ttatcctcta aaatagccag agaaagagcc ttgctggacg tatcaaaagc
     5041 taatactttc ataacacatt cctatctttt tgtctgctta ctattatact acaaaagctg
     5101 gcacatggga attttctttg cccccagaca agagtgccct cacttaacta aaaataattt
     5161 aaaaaaatgc tcacttttcc ttttcttttc cgaatataaa agtgaacaag aaaaaaggag
     5221 gaaagttcaa tgacaaattt tgacattctt gacaatcaat ttttatcctt atctgaaaat
     5281 gaattatcag atattgatgg cggtctcgct cccttggtta tctttggagt agcagtatct
     5341 tggaaggcta ttgcaggtgg aacagcactt ataggttctg gtttggcagc tggttatttt
     5401 ttaggaggag attaatatga tgaaagattt gaacaattat cgtgaaattt ctaataagga
     5461 attgcaagaa atcaagggtg gctttggtgt cggtgttggt atcgctttat ttatggcagg
     5521 ttataccatt ggaaaagacc ttcgtaaaaa gtttggtaag tcatgctaga taagaaacac
     5581 atttttagaa ggataaattt tattgtcttc atctcttaca gtttgctcag cattctcaat
     5641 gatttgaaca ttactaccat ccctttacca ttcgatttat ctgtttgtat tgttttattt
     5701 ttatgcttca actctatttt tgatcagaac aatgactccc ataaaaataa taagctttga
     5761 aaattccatt gtcatgtcat gttagaaaaa tgcaaagacc acctcatctt gatagatggg
     5821 gtggaatttt cgtgtcgtaa atctactatc tctacatccc caaacaaaaa accccagcat
     5881 aagcagggca tctaagcatt taattcaaag taaaatacaa accaaacgac ataggtcacg
     5941 aggaggagaa aaagcgagta gagagtcaca aaggtcattt tccacaagaa cttggtttgt
     6001 cgtcgttcca gtttggcaaa tagaagattc cccgcataaa cgcaagcaac aaaaacaata
     6061 aaagctacca agcgagctcc gatagcaaaa gcaaataagt tatacatagg gcaacctcct
     6121 tgacttaaaa tctatatgga attatgacaa gcaataaatt tcacttccgt tatcaacata
     6181 atacattttc tttattcttg caaacgctta ccaaagaaat cgtcccctaa ctttctcgtt
     6241 tccgtctttt actaattttt cattttgtgg tataattgaa ataattgtaa cgaatcaagg
     6301 tcaatctaga cacaaaatgg aatgaaatca agcaaatatc tgctaaaagt ttggaataag
     6361 ctgacctgta aatagaaagg aactatatga tttacaaagt tttttatcaa gaaacaaaag
     6421 aacgtagccc acgccgtgaa acaacacgcg cactttacct agacatcgat gccagctcag
     6481 aacttgaggg tcgcatcact gctcgccaac ttgtcgaaga aaatcgccca gagtacaata
     6541 tcgaatatat cgaactcttg tctgacaaat tgctcgatta cgaaaaagaa actggcgcct
     6601 tcgaaatcac ggagttctaa tatggcctat actcttaaac ctgaagaagt cggcgttttt
     6661 gccatcggtg gtctgggaga aatcgggaaa aacacttacg gaattgaata ccaagacgag
     6721 attatcatcg tcgatgctgg gattaaattc ccagaagatg acttgcttgg tatcgactat
     6781 gtcattcctg actactctta catcgtggac aatatcgacc gcgtcaaggc tgttttaatc
     6841 acacacggac acgaggacca cattggtggg attccgttcc tactcaagca agcaaatgtc
     6901 cctatctacg ctggaccgct tgccttggct ttgatccgtg ggaaactcga agaacacggc
     6961 ctcttgcgca acgccaaact ttacgaaatc aaccacaaca ccgagttgac ctttaaaaat
     7021 ctcaaggcaa ctttctttag aacgactcac tctattccag agcctttggg gattgtcatt
     7081 catactcctc aagggaaaat cgtctgtacg ggtgacttta agttcgactt tactccagtt
     7141 ggagaacctg cggacttgca tcgtatggct gcgcttggtg aagaaggcgt gctctgtctc
     7201 ctgtctgact cgacaaatgc ggaagtacca acctttacca actctgaaaa agtcgttggt
     7261 cagtccatta tgaagattat ccaaggtatt gaaggacgta tcatctttgc atcctttgcc
     7321 tcaaatatct tccgtctcca gcaggcaaca gaagctgctg ttaagactgg acgcaagatt
     7381 gcggtctttg gtcgttctat ggaaaaggcc attgtcaacg gaatcgatct tggctacatc
     7441 aaagctccta agggaacctt tatcgagcca aatgaaatca aagattatcc tgcaggagaa
     7501 gttcttatcc tctgtacagg tagtcagggt gagcctatgg cagctctctc tcgtatcgcc
     7561 aacggaaccc accgtcaagt acaattacaa ccaggtgata ccgttatctt ctcttctagt
     7621 cccatccctg gaaacactac tagcgtcaac aagctgatta acatcatttc tgaagctggt
     7681 gtcgaagtta tccacggtaa agtgaacaat atccatacat ctggacacgg tggtcagcaa
     7741 gagcaaaaac tcatgctctg cttgattaag ccaaaatact tcatgcctgt ccacggtgaa
     7801 taccgcatgc aaaaagtcca cgctggacta gcagtggata ctggtgttga gaaggacaat
     7861 atctttatca tgagcaatgg cgatgtgctt gcccttactg ctgactcagc tcgtatcgca
     7921 ggtcatttca acgcccaaga tatctatgtc gatggaaatc gtatcggtga aattggcgca
     7981 gctgtcctca aagatcgtcg cgatctatct gaagacggtg tcgttctggc agtcgcaact
     8041 gttgacttca aatcgcagat gattctgtct ggtccagaca tcctcagccg aggctttgtc
     8101 tacatgagag agtctggcga cttgattcgc caaagccagc gtatcctctt caatgccatt
     8161 cgtatcgcac tgaaaaataa ggatgccagc gtgcaatctg tcaatggtgc cattgtcaac
     8221 gctattcgcc ccttcctcta tgaaaatacc gaacgtgaac cgatcatcat cccgatgatc
     8281 ctcacaccag atgaagaata aagcaagaaa acagccccgt cctcggagct gtttttctct
     8341 atgctttctt ttgagattaa aactcatact caatgaaaat caaagagcaa actaggaagc
     8401 tagccgtagg ttgctcaaag cactgctttg aggttgtaga tagaactgac gaagtcagta
     8461 gccataccta cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa gagtatcaat
     8521 aaaaatcgaa atcagactag aaggctaagc gaaagcataa cttgagttag ctcccatagt
     8581 tcgggaaact atgggaggct ggagatgaat caaagccaag ctttgaactc attcgtaaga
     8641 agccgacgac gtatcatttt gatttttgaa gagttttaga aatactacga tttttacctt
     8701 ccagatacac catcaaaata gaaatatctg ctgggtttac tcccgaaata cggctggctt
     8761 ggccgatggt ttctggattg atgagtttga acttctgacg ggcttcggtt gcgatagaat
     8821 caatgtcatc ccagtcgata ttggccggaa tgcgtttttc ttccatgcgt ttcatcttgg
     8881 caacctggtc catggctttg gaaatatagc cttcatactt gatttctgtt tcaatcaatt
     8941 cgataatctt gtcatccaag tcttctgcag ctggtccgat gaaggccacc acatcttggt
     9001 aagaaacttc tggacggcga aggaattcct tggctgtcac tgcatcggtc aagggtttga
     9061 agcccatctc ctcaaccttg gcattggttt ccttgactgg cttaagtttg atactgtcta
     9121 ggcgcttcat ctcattatca aattgatttt tcttgatttc aaaacgagcc cagcgttcat
     9181 cgtccacaag gccaatctcg cgtcccatct cagtcaagcg catatcagca ttgtcatgac
     9241 gaagaatgag acggtattca gcacgactgg tcaagagacg gtagggttca atggttccct
     9301 tggtcaccaa gtcgtcgatc atcaccccga tataaccatc actgcgcttc aaaatcaatt
     9361 caggcttgcc ttggattttc agagccgcat tgatacccgc gataatccct tggccagcag
     9421 cttcttcgta acctgatgtt ccatttgtct gaccagcagt gaagagacct gagattttct
     9481 tggtttccaa agtcgcacgc aactgatgag gcaagaccat atcatactca atagcataac
     9541 ctgtccgcat catctctgca ttttccaaac ctttgatgga atgaaccaag tcacgctgga
     9601 catcctcagg cagactggtt gaaagtcctt gaacatagac ttcctcagta ttgcgccctt
     9661 ctggctcaag gaagagttgg tgacgttcct tgtccgcaaa gcgcacaatc ttgtcttcaa
     9721 tcgacggaca gtaacgaggc cccactccct tgaccacacc tgtaaacata ggcgcacggt
     9781 ggaggttgtt ttgaataatc tcatgactgg taccattggt ataggtcaac cagcatggca
     9841 cttggtcctt gacataatcc tcatcacgtg aagtgtatga gaagtgatta ggcgcttcat
     9901 ctcctggttg gatttctgtc acgtcgtagt tgatagaaga ggccttgacg cgtggagggg
     9961 ttcctgtctt gaaacgaccg atttcgagac ccagttcctt gagattgtca gctaggttaa
    10021 tagaagccaa gctgtggtta ggacctgatg agtacttgag gtctccgatg ataatttccc
    10081 cacggagagc agtccctgtc gtcacaataa cagccttagc agcatattct tgatgggtgg
    10141 ctgtacgcac accgacaacc ttgccatctt ccaccaaaat ctcatcaatc atggtttgac
    10201 gaagggtcag attttcttgg ttttcaaccg tcttgcgcat ctccttagag taaagttcct
    10261 tgtcagcctg cgcacgaagg gcacggacag ctggcccctt ccctgtgttt agcatcttca
    10321 tctggatgta agtcttgtca atggttttgg ccatctcgcc accgagggca tcgacttcac
    10381 gcacgacaat ccccttggca gaaccaccga tagagggatt acaaggcatg aaagccagca
    10441 tttcaatatt gatggtcgca agcaggacct tacagcccat acggctagcg gccaaggaag
    10501 cctcaacccc agcgtgtccc gcaccaatta caataatatc gtattcttca gtaaaatgat
    10561 aagtcatgtt tctctcctat tcctcaagat gaatgtgtct tagttggcct tcccaatctg
    10621 gtagggctgt ttttaaaaag gctggaacta gctggatatt ctggagctta tccaagtcaa
    10681 tccactcaca gggctgcctt ttctcatctt cctgcatggt caatggggca tcttcaagca
    10741 aatccaccag ataatgaaac tcgatattgt gataggaaac accgtattgt tcaaaacgat
    10801 tttcaaccac aaaagctagc tgcccagctt gagctttaac acccagttct tccttcactt
    10861 cacggactac cgcgtcttcc gtgctttcat tgacttgaat cgcacctcca atagtgtaat
    10921 acttgccctt gtctttggta actagaagct tgtgattttg gacaatcaag gctgtcgccc
    10981 gaacaccaaa aaccgtattg tctacttttg tccgaaagtc ttgttgagtc attcttgtcc
    11041 tttcccttaa acgacacaaa aacagtcaaa actacaaaga agtgcaggac aaaaaagcct
    11101 gcaacatcca tgagctttga ccatcatttc tattgctgtt attattgtag caaattgaga
    11161 aaatttgtca atctaaatgt actgacaaac ttgtccatcg cggtaagcat tgtcaatcaa
    11221 accaccacct agacactctt cgccatcgta aaagacaact gcctgtcctg gtgtaatcgc
    11281 gcgttgtggt tccgcaaaga tgacctctgc cttgtctcct ttgacatgga cggtcacctt
    11341 agagtcaggc tgacggtaac ggaatttagc cgtacattct agcgtaaact cttctggcat
    11401 ttcacgagta aagtggactt gactggcttc taggctagtt gacatgagcg aatcatggta
    11461 gaatccttgt cctacataga gaatattctt gcttagatct tttccgacaa cgaaccaagg
    11521 ggcattgtca ccgccgtgtt gcccaccgat accgagtccg ccacgctgac cgattgtata
    11581 gtacataaga cctgcatgct cgcccatatc gcgaccatcc acagtcatca tgcgaccagg
    11641 ctgagctggc aggtagttgc tgaggaagtt tttaaagttc ttttctccga taaagcaaat
    11701 ccctgtcgag tctttcttct tagcagtcga aaggcctgct tcttctgcta gttttcgaac
    11761 ttcaggcttt tccaaatgtc ctagtgggaa catggttttt tgaagttgtt cttgcgaaag
    11821 ttggctgagg aaataggtct ggtccttgcc attgtccacg ccacgaagca tgtgaacagt
    11881 gccgtcctca tcacgcgcca ctcgagcata atgcccagtc gctacatagt ctgcccccaa
    11941 ggttatggca tagtccaaaa aggccttgaa cttgatttcc ttgttgcaca taacgtccgg
    12001 atttggcgtg cgccctgcac ggtattccgc taggaaatac tcaaaaacgc ggtcccagta
    12061 ctctttttca aaattgacag agtagtaggg aatgccaatc tggtctgcca ccgcaaccac
    12121 atccttgtaa tcttcggtcg ccgtacagac gccgttttca tctgtgtcat cccagttctt
    12181 catgaagata ccgatcacat cgtagccctg ctccttgagc aaaagagccg tcaccgacga
    12241 atcaacacca ccactcatcc ccacgacaac acgtgtttta gagttatcac tcatggtaag
    12301 tctcccatct attcgtttat tcacgattga aggtcgtgtg tgcttcacga ttgaaggtcg
    12361 cttgagcagt attcattata acacgcttgg ttagagaaga caaggaagag cttaaaaatt
    12421 gttttttaaa acttatagtc aatatacaga ttatgtgaat gacgtgagta taccttcttc
    12481 ttatcttcat tcaaataact ataataaata aaacaaccca ttttctctaa tctatttttc
    12541 aagttagaga aaaggagtcg ttttttaatg acatttcctg aaatcataag acaggtacct
    12601 aaagaatacg aacataaatc tattcaatcg aaccttatct ttgtttcaaa attaaaatgt
    12661 caaatgttct taacatgatt taatttaatc ggcttaaacc cattcaccgt tttcatttac
    12721 agtatatccg tctacagttg tgttgactgc aagggcacct aagcc
//
LOCUS       DAVKFH010000052        12424 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKFH010000052 DAVKFH010000000
VERSION     DAVKFH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.052"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(37..384)
                     /locus_tag="VW006_001600"
     CDS             complement(37..384)
                     /locus_tag="VW006_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9380421.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(495..2270)
                     /gene="spxB"
                     /locus_tag="VW006_001601"
     CDS             complement(495..2270)
                     /gene="spxB"
                     /locus_tag="VW006_001601"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9380422.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2477..4720)
                     /locus_tag="VW006_001602"
     CDS             complement(2477..4720)
                     /locus_tag="VW006_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9380423.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4730..5101)
                     /locus_tag="VW006_001603"
     CDS             complement(4730..5101)
                     /locus_tag="VW006_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9380424.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(5112..5507)
                     /locus_tag="VW006_001604"
     CDS             complement(5112..5507)
                     /locus_tag="VW006_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9380425.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5691..5789
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5873..6664
                     /gene="thiD"
                     /locus_tag="VW006_001605"
     CDS             5873..6664
                     /gene="thiD"
                     /locus_tag="VW006_001605"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9380426.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6874..7506)
                     /locus_tag="VW006_001606"
     CDS             complement(6874..7506)
                     /locus_tag="VW006_001606"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9380427.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7499..8302)
                     /locus_tag="VW006_001607"
     CDS             complement(7499..8302)
                     /locus_tag="VW006_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9380428.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8302..8826)
                     /gene="thiW"
                     /locus_tag="VW006_001608"
     CDS             complement(8302..8826)
                     /gene="thiW"
                     /locus_tag="VW006_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9380429.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(8831..9523)
                     /gene="tenA"
                     /locus_tag="VW006_001609"
     CDS             complement(8831..9523)
                     /gene="tenA"
                     /locus_tag="VW006_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9380430.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9534..10184)
                     /locus_tag="VW006_001610"
     CDS             complement(9534..10184)
                     /locus_tag="VW006_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9380431.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWIIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10186..11571)
                     /locus_tag="VW006_001611"
     CDS             complement(10186..11571)
                     /locus_tag="VW006_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380432.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11572..12132)
                     /locus_tag="VW006_001612"
     CDS             complement(11572..12132)
                     /locus_tag="VW006_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9380433.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3730 a         2778 c         2383 g         3533 t
ORIGIN      
        1 aaaatagcta aggttaacgt caaatgacta cgcgacctat ttcatacgat aaaaatcaag
       61 cactagacca gcaggtcctt gaactaataa ggactctgtt ccccaatcgg ttacagttgg
      121 tccgtgtaaa acctttatac caagctcgtt caaccgtttg tagttctggt ctacatcctc
      181 aacctcgata tgaataatga ttcctgactg aaagttttcc aaaggaacca aatgattttg
      241 tgacaacata aggcagtgac taccaatcgt aaactgagca aaaccatcat cagcataatc
      301 tgccttttta tccaaaatat gctccaagtc agcacagact tggggaacat ttgaaacgat
      361 aatatctaat tgatttaaat tcatttactc tcctccataa aaagaccgga ttgctccgat
      421 cttttaaagt tctgctctat gaaaatcaaa gaataaagtc tacaagtttc atatttgatt
      481 ttcggcgaga ggaattattt aattgcgcgt gattgcaatc cttcttcttc caagaagaga
      541 cggaatggta cgagttcttc tgcttcgtat ttttccttga aggctttgat agcttcttct
      601 gagtgaagtt ttggatccaa ttcaagtact tctactggaa gtggacggtg ttgagtgatg
      661 cgagcatcga tgacaacagt tttaccttct ttgttcaatt taacagcttc tgcaacaact
      721 gcatcgatgt cttcgatacg gtcaactgtg aatccaacag ctccttgagc ttccgcaatt
      781 ttagcgtagt cagcgtttgt gaagtctaca ccaaacaagt gtttgtttgt atcttcgtat
      841 ttgttcttga tgaagccgta ctcagcattt gagaagacaa ggttgataac tggaaggtcg
      901 tattgaacgt ttgtgataac gtctgggtag cacatgttga atgctccgtc acccatgatg
      961 ttccatactt ggcgatctgg attgtctttc ttagcagcga taccaccagg aagggcaata
     1021 cccattgtcg caaagagtgg agatgtacgc cacatgttct taggtgtcat gtgaaggtga
     1081 cgagtagatg tttgagtagt gttacctacg tcgattgagt agatagcgtc ttgatcagca
     1141 tgtttgttga ttgcattgta aacttgatac aattgcaatt caccctcagt tttaccttcg
     1201 agtttgttca tgtaatcacg ccagttttgg ttgttcttaa cgtttgcacg ccaccatgga
     1261 gttgattcaa ctgggtttac tttgtcaagg atagctttag ctgcttgacc agcatcacca
     1321 aggattgaag cgtcaagggc atgacgttta ccaagtttgt aagggtcgat atcaacttgg
     1381 atgaattttt cagtgttctt gaatgcttcg taaacttcag caaatgggaa gtttgaacca
     1441 aggaaaagaa ctgtgtctgc ttcaaagacc acttcgttgg ctggtttcca accaacacga
     1501 taagcagaac ctgtcaaacc ttcatagttc cattcgaaag cttcaaagtt tttaccagtt
     1561 gtgatgattg gtgctttgat tttacgtgac aattcagtaa tcacttcacc agctttaaca
     1621 ccaccaaatc cagcatagat aactgggcgt tcagcattgt tcaagatttc aacagctttg
     1681 tcgatttcaa cttcgttcaa agcaggagcg atgaatgagc gttcgtatga acctgaaccg
     1741 tagtatgagt tttcgtcgat ttcttggaaa ccgaagttta ctggaatttc aacaacagct
     1801 ggaccttttt tagaaactgc agcacggcag gcttcgtcaa ttacttttgg caattgctca
     1861 gcgtaagcta cacgtttgtt gtaaacagcg ataccgttgt acattgggtt ttggttaagc
     1921 tcttggaaag catccatgtt caattcgtta actggacgtg atccaaggat tgctaggaat
     1981 ggagtgttat ccatagctgc atcgtaaaca ccgttaatca agtgagtcgc acctggacca
     2041 cctgaaccaa ctgcaacccc gattgagccg ccgaatttag cttgcataac cgctgcaaga
     2101 gcacctgtct cttcgtggcg aacttgtaag aagcggatat ctttgtcttc agccaaagcg
     2161 tccatcaatg agctgagtgt tcctgatggg ataccgtaga ttgtatctac gccccatgtt
     2221 ttcaatacgt taagcattgc tgcagatgca gtaattttcc cttgagtcat aatgataact
     2281 ctccttcaat ttttttagac ttggagaata cgattacata gaattggaaa cgttctccaa
     2341 ttttttacta ttccactgta tcatatttat gctgactttt ctaaaaatct gctcaaaact
     2401 ctctattctc tattctaata cagttttgaa agtgtcgtca tttctgtttt ataacaaaaa
     2461 aatctagtca ttacttttag tctattttac taaaatttaa cagaagggaa ctggtcagaa
     2521 cagatacaga actaaaggcc atggctagac ctgccaattc tgggttgaga gccagtccaa
     2581 cacctgaaaa gactcctgct gcaatcggaa ttccgacaac attgtagata aaagcccaga
     2641 aaagattgag cagaatacga tgaaaggttt tcttactcat gtcgaaggca cgcaccactc
     2701 ctaaaagatt attggttgtc aacaccaaat ctgctgactc gatggcgata tctgttccag
     2761 ctcccatagc aatccccaca tctgctacac taagggcagg agcgtcattg atcccatcac
     2821 caacaaaggc tactttacct gactgttgca gtttatggat ttcatgggct ttttcttctg
     2881 gcaagacgcc tgcaatgacc tcttcaattc cgatttgatc tgcaatagca cgcgccacac
     2941 cagcattgtc tcctgtcagc atgactgtcc cgagaccacg tttttttagc tgactgatgg
     3001 ctagcttagc atttttctta ggaatatctt gcaaagcaag caagcctttg atttcattgt
     3061 caacagctaa gaacacaact gtcttagctt ctttttctag ttcttctagt ttatcttgat
     3121 aagtattaga aatatccatg ccatccagca ttttagcatt tccaagtaaa acttgttttc
     3181 cattgattcg ccctgaaaca cctttcccgt gcaaggcctg aaaattttca acagtttgaa
     3241 actcaagtcc agcttcactc gctcgcttaa cgatagcctc agccagtggg tgttgagaag
     3301 catcttccaa ggaggctgcc aatccaaaca cttctacttc gtcgccgatg acatctgtta
     3361 ccacaggttt cccttccgtc aaagtcccgg tcttatcaaa gacaagggtt tgaactttct
     3421 ggatttcctg taagacagtt ccatttttga ggagaacccc catcttggca ctacgtcctg
     3481 tccccaccat aagggctgtc ggtgttgcaa gtcccaaggc acaaggacag gcgataatca
     3541 aaaccgccac tccgtagaga agagaggaca caaagctagc tccaagcaca accacactat
     3601 ccctgagcaa gacgaaccaa acccaaaagg tcatgattcc taaaatgaca actactggga
     3661 caaaaatccc tgaaatctta tccgtcaagt cctgaatcgg cgcacgactt gtctgagctt
     3721 tcttcacaaa atccacaatc tgagccaaaa cagtctctga gccaactttt tctgctctaa
     3781 agacaagcgt tccactatga ttgatggttg agccaatgac agtatctcca actgtcttgt
     3841 ccacaggcag actctcacct gtcaccatgg attcgtcaat actagagaca ccttctacta
     3901 cgacaccatc aacagcaatc ttttcaccgg gacgcactcg aatcaggtcg cctaccttga
     3961 cttgttccaa aggaacttgg acataactat catcactcaa gacttctgcg gttttagctt
     4021 gcaagtccag taatttctcc acagcttggg acgtattttt tctcattttt tcctcaaaaa
     4081 ctgctcccaa aagaacgaaa aagaggataa atccagcact ttcgaagtaa acagggagac
     4141 cagcaaagag agcaactagg ctatagaaat aagccactag agttcccagc gcaaccaagg
     4201 tatccatgtt ggcattgtgc tttttaaaac tggcccaagc actctggata tatggcttac
     4261 ctgcaactaa cataataggc gttgttgcta gaaaggttcc ccaatgcatg acttgatgac
     4321 taatgctacc tgccaacatc ccaatcatga gaatcacaag aggcacagta cagatactag
     4381 taatccaaaa acgttgcagg agagatagag attttcgagt cttctcaacg actgtatagc
     4441 ttcccttttg catcttcatg ccacaagaaa attcatgtcg ccctaattct tgaggtgtaa
     4501 aacgaatgac tttctcctca tctacgccga ttggttccaa gataccttct tcttcaaaca
     4561 gaatttcctt ataacagttt gaaggagtag cacgatgaaa ggtaatctca gctggaattc
     4621 ccttttgaag ctggatatgg gctggatgat agcctttttc agcttggata cggatttttt
     4681 gaatgccatt ttctaagctt gctttcacaa tttctgtcat agtctccacc tactctacaa
     4741 tcatcttgcc gtgcatcatg ttcataccac aagcaaagct aaactctcca gcctgttcag
     4801 gcgtgatttc cactacatac tcttccccca ttggcaggtt cgcatgtaca ccaaaatctg
     4861 gaaaaacaat ttgatccaga catggtgaag gatccttgcg gtcaaagaca atgcgggctg
     4921 gcactgattt cttgaggaca atcaactcag gagtatagcc tcccatgact tccactcgaa
     4981 tctcttggta tccgtttttt tgctgggctt tttgtccaga tttttcaggc tttttgaaaa
     5041 accaaaacaa gataaacgcg ataagggcaa tacaaataat ggttacaata ctatttaaca
     5101 tgacgtctcc tttacataca attacatctt acttctgtta cagcacttga tttcttctct
     5161 gaaatcacag cttccaagtc ttccaagtca gtctgagtaa attcacattc tacaatcaag
     5221 tcagccaaca aattcctaat cctacgggaa caaaccttgt ctttgatatc ttggacaagt
     5281 aaatcccgac tttggtctag agttaaaagc gctgaataaa caaaggactt gccttctttt
     5341 ttccgagtca aacactcttt ctcaactaga cgagccaaaa gtgtctgaac cgtggacttg
     5401 gaccagtcaa accgctctgc caaaacccta atcaaatctg tactggtctg ctccccctgc
     5461 atccaaataa tcttcatgac ctgccattct gcatctgaaa tctgcattac catacctcca
     5521 aaatctacat ttgtcaatta cactcaacag tatactccca aaatctacat ttgtcaatta
     5581 tagaaataat attttcttcg aaaaatagaa ttttaatcat ttgaaaaacg atttgcagtc
     5641 aaatattact atataaacaa taaaaatatg ctatactaaa gaaaaaagaa aacaaccact
     5701 aggggtgcgt aaagctgaga ttaacgactg ttagatccct ctgactcaat ctaggtaatg
     5761 ctagctgatg gaagtggaaa tgataatggg gactagcagt cttctattgc ctttctaaaa
     5821 cagactagct tgttcttaag aatacaaact tcagttggtt gggaggtttt agatgactta
     5881 tttacccgtt gctttgacca ttgcagggac tgaccctagt ggtggtgctg gcattatggc
     5941 agatttaaag tcattccaag cgagagatgt ctatggaatg gctgttgtaa ccagtcttgt
     6001 cgctcaaaat accagaggtg ttcagctaat cgagcacgtt tctcctcaaa tgttgaaagc
     6061 ccaattggag agtgtctttt ctgatattcc acctcaggct gtaaaaactg gaatgttggc
     6121 tactactgaa atcatggaaa tcatccaacc ctatcttaaa aaactggatt gtccctatgt
     6181 ccttgatcct gttatggttg ctacaagtgg agatgccttg attgactcaa gtgctagaga
     6241 ctatctcaaa acaaacttac tacctctagc aactattatt acgccaaatc ttcctgaagc
     6301 agaagagatt gttggttttt caatccatga ccccgaagac atgcagcgtg ctggtcgcct
     6361 gattttaaaa gaatttggtc ctcagtctgt ggttatcaaa ggcggacatc tcaaaggtgg
     6421 tgctaaagat ttcctcttta ccaagaatga acaatttgtc tgggaaagcc cacgaattca
     6481 aacctgtcac acccatggta ctggatgtac ctttgctgca gtgattactg ctgaactagc
     6541 caagggcaag agtctttacc aggcagttga taaggccaag gcctttatca caaaagctat
     6601 tcaagatgcc cctcaactcg gtcatggttc tggtccagtc aaccatacaa cttttaaaga
     6661 ttaagaaaaa aaactctcta gttcccactt taagggaatt agagagtttt tatactcttc
     6721 gaaaatctct tcaaactacg tcagcttcca tctgcaactt caaaacactg ttttgagctg
     6781 acttcgtcag tcttatctaa aacctcaagg cagtactttg agcaacctgc gactagcttt
     6841 ctagtttact ctttgatttt cattgagtat taattaggaa agaatgtcat gcaactttgt
     6901 taaaaaggct tgcgtttttg cctcaatatc ttctgcttgc atcaaatcac gtacaacagc
     6961 tacaccagct atgccagtgc ccataagctg atcaatattc tccgaagtca agccaccaat
     7021 agcaactatt ggaatggcaa ccgtttggca aattgttttc aaggttgaaa tcagggtgat
     7081 gggtgcattt tccttggtgg ttgtcggaaa aatggctcct gtccccaagt aatctgcacc
     7141 tgatttctcc gcttccagag ctcttttaac cgttttagcg gtgacaccga ggattttttc
     7201 aggacccaag acttgtcggg caaccgaaac tgggagttcg tcatctccaa tatgcagacc
     7261 agcagcatca accgcaagac aaacatccaa ccgatcatcg attatcaagg gtacctgata
     7321 agcatctgtt atttccttga cttgttttgc cagttgataa tattgattgg ttgtgagatt
     7381 tttttctcgc aattggacta tggtaacccc tgaacggcag gccgtctcaa cttttgcaag
     7441 aaagctttcc acggaatctt gatagcgatt ggttaccaga tatagtctaa gtgcttctct
     7501 attcataaac ctctcctttg atggtatcta gccaattttc atctcttctt aggagcgaaa
     7561 gctgattgag tacttggtaa cgaaattctt ccaatcccat tccttgaaca actattttct
     7621 cagcagcgat attgagataa gagactgcta agcaagaagc ttcaaaacca gtctttcctt
     7681 ggctgagaaa aacggctgtt aaggctccaa ccaaatctcc tgtccctgtt atccagtcta
     7741 attctgcaca gccatttccc agtacagcga cctgattttt cgaaacgacg aggtccttgg
     7801 gacctgtgac taagaaagac ataccatgat aggtctgaca ccagtctttc aagacttgaa
     7861 gcaaatcctc cgtttcttga tctttagcac tcgcatcgac cccaacgccg tggtgcttta
     7921 atccaacaag acttcgaatt tctgacatgt ttcctttaag gaccgtaggt ctatagtcta
     7981 aaaggtctct gactaagctc ttacgaatgg atgaagctgt tacgccaacc gcatctacta
     8041 ccattgggag acaaacttga gctgcataag aagctgctat acggattgct ttctccttct
     8101 cagctgacaa atgccccaaa ttgatgaaga gagcctgact ttgcttagta aaatcaagaa
     8161 cctcacgggg atcatctgcc atgacaggtt tgcatcccag agccaaaatc ccatttgcca
     8221 gcatctcaca agaaatctca ttggtcatac agtgaatgag ggaactagag cctataggaa
     8281 agggatttgt aaattcctgc atcagtctat cctttcacta aagaaatatc cctgcacttt
     8341 tttaaagaat tcctgcttga ttaaaaatcg aaaggcaata aaggaaatcg ctgtaccaat
     8401 caaggttgct ccgaaaaatc gaggcgtgta gataaaccag ctaagcttag cagctgatcc
     8461 tgtaaagagt accataacag gataggaaac aatagaacca ataatacctg ttcccacaat
     8521 ttctcccaag gcagaaaagt aaaattttcg accgtactta taaaagagac ctgctagaag
     8581 ggctccaaaa gtcgctcctg tgagagctaa aggcggaatc ccttgagtcg tcatacggat
     8641 aaaggctgtc actgtagcca tagccaaggc ataaacaggt cccatcatga ttcccgctag
     8701 aatattgact acactggaca tcggtgccat tccctcaatc cgaaagatag gtgtaaggac
     8761 tacatcaagg gcaatcatca tagataaaat ggtcaatttg tgaacttgta gttggtgctt
     8821 tctcatgttt ctattcttct cctttttcta aagactgtaa atcgctcttc catgtctggt
     8881 gttggtaagc catctcccaa aacttggctt ccatatgaac actgatgtgg aaggcatcta
     8941 gcattttttg cttatctgtc tcatcacttt ctcgatagag ctgattgacc agtgctccct
     9001 cctctctgat ctgttgctct aactcatccg taatataagt ttcaatccat tgttgataga
     9061 gaggatttgg tgatggttta agattaagtg atttgcctat atcatggtat aaccaaggac
     9121 aaggaagcaa gcttgcaaaa gcgatggcta agttcggttc tgcaaattgc ctataaatat
     9181 gagaaatgta atgataacag gttggagcga ttggatgttg ctccatttcc tggtcgctga
     9241 tttccaattc cttgaaaaat tgttggcgaa taaataactc accctccact aaaccctgag
     9301 cattttgttt caagagtctt ttcatctctt ggtttgaagt cttatcagcc aaaagatgat
     9361 agatttctga gaaagccttc agatagtagg catcctgaat caggtaatag cggaaaatgg
     9421 caggttctaa attcccctct tgtaattgta aaataaaggg atgatgaaag gaagcctgcc
     9481 aagctttctt ggataattcc atcgcaatat ctgtaaattc cataataact cctttataaa
     9541 aatagactgg tttgaagcaa taaaaagaaa agcaggtaga ttaattttgt ttttttagga
     9601 atataaaaag tccgatagct attcttcaac tgtgcatgtt cgtcatatcc gtgagcagat
     9661 agagctctca ggtaaagatg gcgccaccta aagactgtca tcagaacctt actgtaaatc
     9721 aagggcgacc aaaaatgtag ttcttgacca cgtaataggc aagcttcttt gagggacttg
     9781 atttcttgct gaatgagagg aaaagaattg aataccacaa tcaaggcata ggaccaagag
     9841 cgtgatagcc ccttttgagc caagtacaag agaagctctt ttagtgaaat agaggaaaca
     9901 aagacaaggc cgatacaaac tgtcacaaag gccctcgttc caagcatgac tgcctgtgaa
     9961 gcatctccgt gtaactgaac tgcccagtag ttggcaaaag atggtaaaat ggcaagtatg
    10021 attatccaag ctaacatttt aaatcgacgg taatagagca taaagagaat acaaaatgcg
    10081 actaccgaaa gattcagagc aatcgaagga atgaaagatg tttccaagga taaaatcagc
    10141 aagaagagac tgataatcgg tgtctgggtt gctactttga ccatactatc tcacctcccc
    10201 ttgggtattg ctactctgag atgtaagtgg tttggtaatg gtcacttctt tcacatgccg
    10261 aagaccctga ctagtcatct caatccaata atcaaccaca gaaatcaaag ggtctaaacg
    10321 atgactaatg agcagaaaac ttcttccttg attcctctcc tccacaatcc acttgcaaaa
    10381 ataatggcag gctctatcat ccaaacctgc aaaaggttca tctagcaaga tcacggaagc
    10441 cttactggtc aagatggtca ggagctgaag aattttttgc tgaccaccac ttaattgata
    10501 gggactctta tcgactgcct gctccaaatc aaaatatcgt aaagcttgaa aaatccgctg
    10561 atttctttca gaatcaggtc catctaattg aagctcctct cgcagactga ctcggataaa
    10621 ctgcttctca gcttcctgaa caacaccagt cagatcacga tacaaactct ttttcttttt
    10681 caggaccgaa cccttccatg caatgcgccc cttatacttt tgaaattgaa gaatagaccg
    10741 aaagagggtt gatttcccga caccattgtc acccaggata caggaaatcc cttgatagaa
    10801 tgtgaaatca gcaattgaaa agagggggcg atttccaaac tcacaagtcg ttctatccat
    10861 atggaatagt tccgggctag aagcaacttc ctttgaagca acctgtgtca tctcatagga
    10921 agggatttga aacacttccc ttagttttcc gtctcttagc tcaaccatat ggtcgatata
    10981 ggctttatag tcagataaat catggtcgca caaaataact gtctttccat caagagccaa
    11041 ctcttttaga atctccaata tctcgattct gctcttgcgg tcaatggaag caaaaggttc
    11101 atccaagaga tagaccctag gattcatggc aaagaggaca gccagcgctg ctttttgctt
    11161 ttccccacct gataggtgat gaatgggacg gtgtaagatt gcctcgcaac gacattgctg
    11221 gacaacctct gctattttag aatcaatttc ctgaaggtga tagccgatat tctccaaggt
    11281 gaaaatcagc tcctcaaaca agctctccat ggtaaattga tgattgggat tttgaaagag
    11341 aatgccaacc ttctggacac gtgcgatgat agaaagttga ctgacctcgc tcccatctat
    11401 caggacttga ccactatagg gaagagaact gacttgggca atcatttgaa agaggctgga
    11461 ttttccagac ccactactcc caactaacaa ggtaaaggct tgcgcatgaa aagtaaaatc
    11521 aaacggctca gagaagattg gggactgaat cgctcgtagt tccagaccca tctatgcttt
    11581 tcctccagtt gcaaactgat gatagagttt gacaatggca cgaaccaaga tggtacagaa
    11641 gaaataaaca gaaataaaac gtaccacaag taaggaaagg acaaacggaa gggaaaaggc
    11701 gtagtaacct aacttaatgt attcatagac aaagctaaca agcgtaatcc caatactatt
    11761 agcagttaga gagagccaac tttcatagcg attcttagtt acgataaaac caaattcact
    11821 tcccaaacct tgaaccaagc cagacaaaag agctcctaga ccaaattggc taccataaag
    11881 gacttcagca agcgcagcta gcacttctcc aatcgttgca cttccgactc tcggaacaaa
    11941 gatggcagca atgggcgcag ccatacacca gagaccgaag aggatttcat tggcaaaggc
    12001 ctgcaaacca agaggtgtta agagtagact gagaatatta tacacatatc ctgaaccaac
    12061 gaaaacccca ccaaaaaaga tagacaagaa agcaagcaag ataacatctt ttaactgcca
    12121 ttttttcaac ataaaaaact ccttttttta aagaaaagtg gggcactcaa gaagaccgac
    12181 ctaaatactt tgtatagcag actgaattta gaacagtaca caagaatgct aaaatatttc
    12241 tagaaattaa tttgaatttt ctaattgatt tgttcgcatc ttatttcaat ctactatatc
    12301 atcttcatcc agtttcgtaa aagaaaaaac tctaattaca gatacgaatt agagttcagc
    12361 ttacaagatt agacagttct tttcgacata cgaaaaaaac atttcacatt tcccttcgcc
    12421 agtc
//
LOCUS       DAVKFH010000053        12405 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKFH010000053 DAVKFH010000000
VERSION     DAVKFH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.053"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            119..283
                     /locus_tag="VW006_001613"
     CDS             119..283
                     /locus_tag="VW006_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9380434.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            367..933
                     /gene="amaP"
                     /locus_tag="VW006_001614"
     CDS             367..933
                     /gene="amaP"
                     /locus_tag="VW006_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9380435.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            945..1115
                     /locus_tag="VW006_001615"
     CDS             945..1115
                     /locus_tag="VW006_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9380436.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1154..1762
                     /locus_tag="VW006_001616"
     CDS             1154..1762
                     /locus_tag="VW006_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9380437.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1793..1996
                     /locus_tag="VW006_001617"
     CDS             1793..1996
                     /locus_tag="VW006_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9380438.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2106..2384)
                     /locus_tag="VW006_001618"
                     /pseudo
     CDS             complement(2106..2384)
                     /locus_tag="VW006_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3148..3918)
                     /locus_tag="VW006_001619"
     CDS             complement(3148..3918)
                     /locus_tag="VW006_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9380439.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3933..4781)
                     /locus_tag="VW006_001620"
     CDS             complement(3933..4781)
                     /locus_tag="VW006_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9380440.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDNFEDPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTGLDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4778..6253)
                     /locus_tag="VW006_001621"
     CDS             complement(4778..6253)
                     /locus_tag="VW006_001621"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9380441.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVNFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6576..7931)
                     /locus_tag="VW006_001622"
     CDS             complement(6576..7931)
                     /locus_tag="VW006_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9380442.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLNQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWHAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7941..8258)
                     /locus_tag="VW006_001623"
     CDS             complement(7941..8258)
                     /locus_tag="VW006_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9380443.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8275..8592)
                     /locus_tag="VW006_001624"
     CDS             complement(8275..8592)
                     /locus_tag="VW006_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9380444.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8607..9503)
                     /locus_tag="VW006_001625"
     CDS             complement(8607..9503)
                     /locus_tag="VW006_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9380445.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9506..10767)
                     /locus_tag="VW006_001626"
                     /pseudo
     CDS             complement(9506..10767)
                     /locus_tag="VW006_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            11047..12225
                     /locus_tag="VW006_001627"
     CDS             11047..12225
                     /locus_tag="VW006_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9380446.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         4299 a         2459 c         1905 g         3742 t
ORIGIN      
        1 caaagaaatt gattttttga tattaaaata aaaaaggagg gtaggcagtg ttgtgatcaa
       61 ttattgctgg aggtcttatt ggtctcttgg caggtaaaat cactaaaaaa gtagttctat
      121 gggaatcatc gcaaatgtat tcgctggttt agtcggggca tatgcaggac aatctctttt
      181 aggtagttgg ggtccatcaa tcgctggaat ggctttgctc ccatctattg taggtgcagc
      241 gattgtgatt actgtagtgt cattctttac aggtagaaag taaacttttc gccagtaaag
      301 ttagcaaact atttttaaat caatgacggg aaaaatagtt taaatgttaa atcgaaagga
      361 ttgtatatgt caaaagcaaa gaaaatatgt ttcattattt tctgtatttt aatcttgaca
      421 attttccttc ctgttttgat agattatcat caagttagtg atctaggtat tcatctactt
      481 agctggagac agaactccgt agttgaattc tatcttgcta gatatgtctt ttgggggaca
      541 gtggttctat caactttagt tttattatcc attttagttg tgatgtttta tcctaaacgt
      601 tacttggaaa tccaacttga aactaaaaac gatacattaa aattaaagaa ttcggcaatc
      661 gaaggttttg ttagaagttt ggtgagtgat catagattga tcaagaaccc aactgttcat
      721 gtaaatttac gaaaaaataa atgtttcgtt catgtagaag gtaaaattct tccttcagac
      781 aacatcgctg acagatgcca aataattcaa aatgaaataa ctaatggatt gaagcagttt
      841 tttggtattg agcgtcaagt aaaacttgaa gttgcagtaa aaaattacca accaaaacct
      901 caaaacaaaa agactgttag tcgtgtgaag taaggaagta aaaaatggaa tggcttaaac
      961 aatatcgata tccaattatc gctggtctca taggcgtatt tctggcttgt ttgattgtct
     1021 cctttggctt cttcaaaaca atatttgtat tgattttagg agcactggga gttgcagctg
     1081 gattatatat cgaaaaaaac tatatagata aataaaaaaa taaaaattac taatttaatt
     1141 aaaggagttt catatgtcaa acgaaaaaaa cacaaacact aacgtagaaa agaaagatgc
     1201 tactgttgta gctcacgaaa tcaaagggga acttacttac gaagataaag ttatccaaaa
     1261 aatcattggt ctttcactag aaaacgtttc aggtcttttg ggaatcgatg gtggtttctt
     1321 ctcaaatctt aaagaaaaaa tcgttaacag cgatgacgta acaagtggtg ttaacgtaga
     1381 agttggtaaa acacaagttg cagttgactt aaacgttatt gttgagtacc aaaaaaatgt
     1441 tccagcttta tattcagaaa tcagagaaat cgtatcttca gaagttgcta aaatgactga
     1501 cttggaaatt gttgaaatca acgtaaacgt tgtcgacatc aaaactaaag aacagcatga
     1561 agcagactca gtaagccttc aagatcgcgt atctgacgtt gctgaatcaa caggagaatt
     1621 cacttcagaa caattcgaaa aagctaaatc tggtcttgga tctggtttct caactgttca
     1681 agaaaaagtt agcgaaggtg tagaagctgt taaaggtgca gcaaatggtg tagtatctca
     1741 cgaaaacact cgtgtaaact aagataaaat aaatataaca ggagaaatta tcatgtcagt
     1801 agaagaaaaa ttaaatcaag ctaaaggttc tattaaagaa ggtgttggga aagccatcgg
     1861 tgatgaaaaa atggaaaaag aaggagcagc tgaaaaagtt gtttctaaag taaaagaagt
     1921 cgccgaagat gccaaagacg ctgtagaagg tgctgtagaa ggtgttaaaa acatgttgag
     1981 tggcgacgat aaataaggtt aaaagttact ttatcttttt agtaacatta gtcaaaagag
     2041 tctgagtcaa gatgattctc agaaaacaaa aagctagaga ttcccaattg cggaactcta
     2101 gctttttaat tttgcctctt tctcttatta tatttcagca ggttgttggc catgagtatg
     2161 aatcccatgt caattctcac ttgacgctta cctctcagat gacatctctt ataacccaaa
     2221 caaaccttta tctgcccaaa gacagatttc atatcaatct tacgtttagc gaaaatttgt
     2281 ctacccttgg aagataaaag tgcctgatat tctttagttt ttaaacactg gtaacgttca
     2341 ttcatataca gtcccttttg aggggctgat tcaggttcat aatcgcagtc aacattgatt
     2401 tcaaggctgt ttgctttcta tctccccttt ttcatgatat attttcgtac aggtgcttca
     2461 atagtttcta tagtaaaatg aaccaaaaat agtacacgtt gcggtataat gttcttgtgg
     2521 catattcatt agattttcgt aaaaagttct ctcctatttg tgagcgaaca ggtatagtca
     2581 attgattgtc agtttgaaga ccaagagtcg taaggcaaag gatatggcag aatccatcca
     2641 agggtggctg gcacaatttc tagtgaacct ctttaaatct attacgtttg actgtggaaa
     2701 agagttttca aaatggaaag atatttccaa tcatcacgat agtgagagct tttttgcgaa
     2761 tctaggatgt ccaagatagc ggtgtctcaa cgaacattca aatagacttt taaggtgtca
     2821 tgacttgcca aagcagacag atttcaacga agtcagtcaa gaattttagg ctgggcaaga
     2881 actcaatttc aggagaaaat gaaataaata ttcccacaat aaaacgcata gtatcaaagt
     2941 ttttccaaca cctgatatta tgcatttttt aattttaaag actttttccc agcctcaaga
     3001 aatcacatcc cacctaaatc ctttggatac caaatacctc atcaggttat tttctgagtt
     3061 ggtaagactg tctagcttaa tttgacaaat ggagggcgtt aaatccagtt tttacaggaa
     3121 gtctatttaa atattttcac actgacctta caaaatatga aataaattat ataaagtcat
     3181 tcccatgaca aagaataaca tccctttaaa acagtatctt acccaatttt caggtaagac
     3241 ttttgataca accgtcccca atacccctcc gcatatagaa gcaagaaaaa taactggaac
     3301 aggtgctaaa tgatactgat ttaaacctgt aaccacaaca attgtagcca aatgagacag
     3361 ctgggaaaag aaaataattg caagagaata catcgttgct tcttttatcg aaatagaaaa
     3421 aaacaccatc aataaagata cattgagcgg acctccacca atccctaaaa aactagaaat
     3481 caagcccaat agaagaccac aaatcatata gaatcctatc ttatcaaatt gataagtttt
     3541 tttaaaatct gttaaaacaa atgatacaag tagcatcaca aatagtaaaa tcatttgaac
     3601 aatggatact agccgtactg ataattgaga taacaaaact tgaaaaataa gatgacctag
     3661 aaatcctcct aaaacagaag aaaaggatac cgtaaaaatc aatctccatt ttatctcttt
     3721 actttgtgaa aatcttttgg ttgttgaaat aatcgccatt ataaaaacag agaaactaga
     3781 ataaaaagca atatcactga ccgaatggta tccaaaacta tccataagtg gcttaattat
     3841 aattccacca cccatgcctg atatagaacc caatccacta gctattataa tgattagaaa
     3901 ataaataatc tcaaccactt tttaagtcca tcctattctt tcaaacatct aaaacctaga
     3961 tggctactag tggaagtaga aatacatcca tttctagcag ctactcggta tcgattacaa
     4021 taagaacaat gacataaaaa ggaacctcct ctgatagcat actcctcatc ttgtatccca
     4081 tattttggca actcatagtc atcttcatta aaactagcta ataaggtata tctcggattg
     4141 cgacaccatt cccaaacatt cccaatcatt tgatatagac caaaatcatt aggtaaaaat
     4201 tcatagacag gagccgttcc tataaatcca tctaagccag tattttcata aggaaatctt
     4261 ccttgccaag tattggcatg ataatatcca ccttctaaaa gagtatctcc ccaaggatat
     4321 tcagattgtc ttcctcctct agcagcgtat tcccactgtg cttcagtcgg taaagacatt
     4381 ccgctccaat tacagaaagc caatgcatct tctaaagcaa catgaactac tggatggtct
     4441 tctaaaccca ctagattact gttctcaccg tagggatgtt tccagcaagc gttcggaact
     4501 tgtaaccacc acggagcccc ggcaggatga ggatatccct ccctttctgc ctccggaacg
     4561 aacaatataa aaacaaaaga ccactcttgt cgctcagcta gtgttttata gcctgtttct
     4621 ttcacaaatt gagcaaattc ttgattcgta actggagtat ccgcaatgga aaaatccttt
     4681 acactaacag cgacttgagg atcttcaaaa ttatctaaaa agccctcttc agaatttgtt
     4741 cccatcataa atgtcccacc cttgattttt ttcattttca tgattgacta tcccccgttt
     4801 tcgttagagt tggcgttatt ttagaaagtt caacaggaac taactcttca tccacaacaa
     4861 aaccttcttc tctatatcta agaaagttta ctaatttttt cttcatctga cgaacaatag
     4921 gttggtattt ttcacttgga tacaaatcat tcatttcatg cggatctttt ttcatatcaa
     4981 ataactgata atgatttaaa acaggaaacc agataaattt ccactggtct gtcaaaatat
     5041 attgactaga atcttttcct aaagcatgtt caccatgaaa ttctgttcgc cacccctcgt
     5101 actgtccaaa taataaattc ttaacactcc gtccatctag ttcatctgta gtagttcctc
     5161 ctgccaaatc cacgagtgaa ggaaaaatat cttgaatttt cactaattgc ttgattgttc
     5221 ccctattacc agctatcaat ccagccggat cgtaaataaa cgaaggaatg tggatacttc
     5281 cctgataagg atatcccttt ctaaacagat aatgttcacc aagctgatct ccatgatccg
     5341 aaacaaacca gataatcgta tctttatcat gacgaaattc ttttagggca gtcaaaaagc
     5401 gactaatttg atggtctata tgagtaatca atccaaaata agcagcaacc attcttcttt
     5461 gatcatcttc ctttaatttc cctcttaaag cataaataga cggaatttgc ttttctaata
     5521 cttcccaatt cccgatatgc aaatcgagaa attgaggcaa gcgttccata tacatatcaa
     5581 aatagtattt tgggggattt aatggagcat gtggtttttc aaaagacatt ttgagaaaaa
     5641 aaggaaccgt tggatctctt ctttgtaaaa atgaaatgga ctcactcaca acccagtttg
     5701 ttggatgtaa cttttcatct ttatcccaag gtctagcttc ccaagaatta caatccattc
     5761 cgtcatcaat caaatcaaca tcataaccaa ctttaccctt taaaaaggct agatagtcac
     5821 tagcataatc aaattgactt ccataagcct tatcatactt tctatcaaca tgtagatacc
     5881 catcgtgaag taagacatga tcaaacccaa gtctctgtct ggaaggaaaa acatgcatct
     5941 taccaataca ctctgtttga taacccatat ctttaaagac ttttggaaga gtattagtaa
     6001 aattccaagg cacctcatct tgataaccaa cacgtccact tttatcttgg tctaagccag
     6061 tcaataaagc tgccctagca ggaacgcaag aaggaactgg agaataggca ttttcaaaat
     6121 tataacctac actagccatc atatctaatg ttggagtgct aactagctta tccttactat
     6181 tcaaggataa ggcgtctgct ctcatttgat ctacaacaat caaaataata tttggctgtt
     6241 ttgtctgaac cataaaatct cctttctaat atggcaaaag aggcacaaga agatatctac
     6301 ctttactgca cccctttcta tatcaatctc tctatataaa acaataacat tcttgttatg
     6361 ttttatataa aaatgaacta aaatatgact aaatcgatta ggaaattcaa atcattttct
     6421 agtactgttt tagaagttac agtgtactat tccaacttca ataaactata aacctttgtc
     6481 tattaacaat tttagtggag ataagaaatc ctacacctaa ctcatcttac acgtaatcta
     6541 tttctatttt atcacaaaaa acgcaagtaa gaccattaac tcaattcagt tttatctgcc
     6601 attttcacaa atgggaaata agtcaagaca ctaataatca aacaaacaac tgataagatg
     6661 atggcatgcc aatcaaatgc tgtagagaag aaaccatata aaattggagg cattacccaa
     6721 gtaacatttt gtgtaacagg tgaaacaaga ccccagcttg ttgcccagta agctaccgtt
     6781 gccatgaaaa ccgggctaag tacaaatggt ataaatagca aaggattcaa gacaactggt
     6841 aaaccataat tgaccggctc accaatatta aacagaactg gtgctagacc aagtttagca
     6901 acttttcgat aatgactgtt tcttgaaaaa attaaaatag caagtactaa tcctaatcct
     6961 ccaaaccaga caaacgcccc aaaagaccca cttgtccata tataaggaat cggttcacct
     7021 ttttggaaag catccagatt cgctaacata gcaactccaa atagcccttc catgatggga
     7081 gccaatacat ttcctccatg gagaccaaaa aaccagaata acttattcaa aaagatcatc
     7141 agaataactg caaagaaact ttgagacaaa cctagtaatg gcgtttgtaa caccttgtaa
     7201 acccaatcaa tcaataagtc attgctaagt aaatggaaaa cataagtcaa gatggctact
     7261 atatacatcg ccataaatcc tggaatgata gaagtgaacg gcttagcaat cgcaggggga
     7321 actgaatctg gtaacttgat tacccagttc tttttcatta ctttacagaa aataatagag
     7381 gctaaaaatc caatcatcat ggctgtaaag tatcctctgg cattaatatg gtttcctgga
     7441 atcacattcc caatagttac catcagattt ttaccatcaa atgctagatt atcaattcct
     7501 tgtaagattt gatttaattt cacatctcct acatttgcca aagggaaact ctttgtaact
     7561 gtacttccaa tcgaaatgac aaacgaagca agtgatacca aaccagcaga aactgtatca
     7621 accttgtaaa tcttagcgat attcactccc aagcaataga tgaacaataa ggaaacaatt
     7681 ggtatacttc ccttgaatac caaattattg atgtcaacaa gccactgaaa ggttttcgta
     7741 atactttcta ggtgaaattg ttgtggtaaa tccactagaa aagcatttaa taacaaagca
     7801 atggaacctg tcataataac aggcatagtc cccacaaatg aatcacgtaa agcaactaaa
     7861 aacttctgat tcccaacttt cattgcaatt ggagaaagtt tttcttgcat tttatccaac
     7921 attgtattca tagcaaattc ctaatttttt tcaaatcgtt cgtatacttc taccacttct
     7981 ttcgccaagt caacaaaggt cagactcgtc attaaatgat cttgcgcgtg taccatcaag
     8041 aggctaattg aagtttcttc cccctgtgcc tctcttgtca acatttctgt ttgagcctta
     8101 tgcgcctgta ataaggcagc attcgcatca gctaatcttc gattggcttt agagaaatcc
     8161 cctttttttg ctgcctgaat agcctccata gcattacttt tggcttcccc accatacata
     8221 attagcccca ttgcagattc taaattactt tcattcatag cattctcctc aatcttagtc
     8281 taataaactt tcagctaaat ctaagacttt agaaccattc atcattccat aatctgtcat
     8341 cggaataacc gccacaggaa tctgtctatc ttttagtttt tcttgaaagt cccctagtaa
     8401 atagcgaact tgagggccta aaagcaacac atttacttcc tttgttgcta caatttcttc
     8461 tgcttcagga gctggaactg caaaaatttc tgcatccaac cccttatctt ctgctgcctt
     8521 ttgcatcttt gtcactagca gacttgtact catacctgct gcacatgcta acataattgt
     8581 tactttagcc atagcttact cctttatcac ctatttactg acctagccaa gcgagatact
     8641 gtatgtctca ctcctctaat tgccttgctc aatgcataaa tattatcgac tgttgccagt
     8701 cccccatatc ctgcgtcacc tatatgttga atatcaactc cacaaatttt atttctaagt
     8761 gcaatttcct tgatagtatc tgtatcagat gtttcttggc tagtaccaat agcactcagt
     8821 actagcccac ccttactatg aacgagatca acgacttcac gcaactcttg gtcatgaaag
     8881 gctggaacgg ttccaactgc tggaacaagt atcacatccg cacctgcttc caacaattgc
     8941 tctgctacag aaagctctgc cacaggctca ttcactcctg ccccgtgcat cttacctgca
     9001 ataatcaaac cagaaaagtt ttccttagca gtttgaacag ccttaatgat ttctcgattg
     9061 ctaactcctg ttccaggatt tccagtcaaa cagacaaagt caaaacctaa ctcctctatt
     9121 cgattcaatg tttcaacact agcaacacga cctgcaacaa tttcctgtgt ttcctctagc
     9181 atctttgcag atgggtcaat cggttccaaa tttacaccaa ttggacaagc aacaagctgg
     9241 tgtaagcgat gaataacatc atcacctgaa ctatccaaag cataaatttt aggctcaaaa
     9301 acatcaacac aattcaataa aatcatatca gctccaaaag ctctagcaat ttcagaatta
     9361 gttatatccc caacaaatgt ctcacgagta actacatttt cagatagaac aactctacct
     9421 tcacttgcta aaatactttg ttttaattct tcagcattca tctgtaatat ctcagatgga
     9481 tttgcactaa ttaaacgttt cattctcaat cctccaataa cttaaaccgt ttttcaatag
     9541 ctattacaaa tggtaaataa agtaagacat ccaaaacaat caatacaatt tgtaataatg
     9601 aaccagaaat acttcctgta gcaagtaaac cacttataac aggtggcata gtccaacctg
     9661 gatctgtata agttagcgga accaaacctg atgccattgc tccccacgct actagtaagt
     9721 taaacattgg agctaagata aatggaacta gtaacaaaat gttcaaaaca ataggaacgc
     9781 caaacattgc tggttcattt atattaaata atccaggtat aacagttata ggtgccaaag
     9841 tttttagttg tttactagct ttcttcttat gtgctaaata tcctaacgca agaactaaac
     9901 ctatggttgc accaccgcca cctatgaata caaaattatc cataaatacg ctagtaaaaa
     9961 tatttggaag agtttctcca gtttgataag ctactttatt tgcatctaga ttagctaacc
    10021 ataacggttt cataactgca ttaacaacat ttcctccgtg taatcctaca aaccaaaaac
    10081 tactattaag gaaaattaag ataagaagac caataacatt atttcctaaa agtccaagtg
    10141 gacctcccaa agctacttga gcaatttcat gaagattagg taaacttaat ttatctaaag
    10201 ttgcataaac aatcaaccaa ccaacaatag tcactgcacc aggaataatg gcactaaact
    10261 tttagatact gctggtggaa caccatccgg catttttatc tgtatattat gatttataaa
    10321 ccattggtag atatagccat taatcaatcc taaaatcata gcaacaaata aaccttttga
    10381 tgccatgaaa gcggtaggca ttcctgctcc agcctctcct ctaatgaaag gcgtaactgt
    10441 aataaaagat gataatgaca caataccagc aggaatacca tcaactttgt attgtctagc
    10501 cataaagtaa gcgataccaa aactagctac aagaccaagt aatccaaaac tactatcaac
    10561 acccttccat aaatagtcag caacaccaat atcacccaga tatttttccc atcctggaat
    10621 aggaaaactt gcaataacca ttagtaatga acctatcaac aataaaggta tagttagagt
    10681 aataccgtct cgaatagaaa ccaacgcttt attattaccc aatctagccg ctactggaag
    10741 taacttttct tgtaataatt tttccataac cttttcctct taaatatatt ttattatcaa
    10801 aagttatcaa ttatctttat tttttgacgt caagttgtta tttgattatc ttttgttatt
    10861 tatattttac aactataaga aagccttgtc aaggatttgt gtaaatattt gattattttt
    10921 tattatcaat atataataaa aagagaccag taagcaatga aaagcttaaa aagattaaca
    10981 tctacattta tccatagcaa aaaatcatag gattttaaga atctgacaac taggaagaaa
    11041 actaatatga ccttatctaa gaaacaatta caactacgag ctaaaatact ggaaacagtt
    11101 tacacattag gtcccatttc acgaattgaa atcgctacta aaacaggaat aacacctgca
    11161 acaactagca gtatcacaaa tgatctgatt aaagaaaaca ttctcttaga acttggtgaa
    11221 gacgaacacg atacaagcgt agggagaaaa aaaatcctgc tagacattca agctaaacga
    11281 ttttactaca ttggttgtga actatctgaa aaacatttca cttttgcttt aggagataat
    11341 ctaggaaata tcttaaagga agagaaagaa attgttacta aacagttaat ccaagaaaag
    11401 ggaaatcaac tcattagcca gacactaaag caattcttaa ataactgctc tgattatgaa
    11461 atagaagcaa ttggaatcgc gctacctggt cgctatttag atgattataa aataacaaca
    11521 aacaatcctt tatggcaaca tatcgactta gaaatgattc aatcccactt tgataagcct
    11581 ttgtttttct ctaacaatgt taactgtatg gctataggta aacgcctatt tagtcgccaa
    11641 cagaatgacc ctaattttgc ttattttcat tttgctagag gtatgcactg ttcttatatc
    11701 tacgacggaa acatttatgg aaaaggaaat ctaatgattg gagaaatagg gcatacagtt
    11761 gtttcatctg agggagaaga atgtagttgc gggcgcaagg gatgcttaca aacttttgct
    11821 ggagaatctt ggttaatcaa aaaatcaaaa attctatatc atcaatctcc ctattctcta
    11881 cttcctagtt tagtcaaaaa tgccgatgat attgatatcc aagttatttt aaccgcttat
    11941 caattaggtg acactggaat aatcacactt atccatcaag ccttactcta tcttagtcaa
    12001 accattttaa atatcagtat gatgattgac tctcaaaaaa tctacctaca tagtccttta
    12061 ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa tgaactacaa acccaaactg
    12121 ctctataatc gcctacctga agtcattatt gaaccttaca atgattttac agccgcacac
    12181 tcagctattg cactttgttt gtatcataca attctacatt cctaattttc tattacattt
    12241 aataaatagg ctaaaaagag ggagtatatc ataaaaagcg aacaaacctg ttttctgaaa
    12301 atcagaattc tgttttgttc gctttcttta tatttggaat tatactccta tctccccttt
    12361 ttcataatgt atagtaaaat gaaataataa caggacgaat cgatc
//
LOCUS       DAVKFH010000054        11956 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKFH010000054 DAVKFH010000000
VERSION     DAVKFH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.054"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(393..1286)
                     /locus_tag="VW006_001628"
     CDS             complement(393..1286)
                     /locus_tag="VW006_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380447.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(2037..2267)
                     /gene="blpU"
                     /locus_tag="VW006_001629"
     CDS             complement(2037..2267)
                     /gene="blpU"
                     /locus_tag="VW006_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9380448.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(2470..2760)
                     /locus_tag="VW006_001630"
                     /pseudo
     CDS             complement(2470..2760)
                     /locus_tag="VW006_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2806..3039)
                     /locus_tag="VW006_001631"
     CDS             complement(2806..3039)
                     /locus_tag="VW006_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9380449.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(3045..4037)
                     /gene="plsX"
                     /locus_tag="VW006_001632"
     CDS             complement(3045..4037)
                     /gene="plsX"
                     /locus_tag="VW006_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9380450.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(4034..4804)
                     /gene="recO"
                     /locus_tag="VW006_001633"
     CDS             complement(4034..4804)
                     /gene="recO"
                     /locus_tag="VW006_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9380451.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(4801..5970)
                     /locus_tag="VW006_001634"
     CDS             complement(4801..5970)
                     /locus_tag="VW006_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9380452.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6119..7129
                     /locus_tag="VW006_001635"
     CDS             6119..7129
                     /locus_tag="VW006_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEU9380453.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(7457..7894)
                     /locus_tag="VW006_001636"
     CDS             complement(7457..7894)
                     /locus_tag="VW006_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9380454.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(7979..10612)
                     /gene="polA"
                     /locus_tag="VW006_001637"
     CDS             complement(7979..10612)
                     /gene="polA"
                     /locus_tag="VW006_001637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9380455.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            10868..11218
                     /locus_tag="VW006_001638"
     CDS             10868..11218
                     /locus_tag="VW006_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9380456.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            11433..11549
                     /locus_tag="VW006_001639"
                     /pseudo
     CDS             11433..11549
                     /locus_tag="VW006_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            11542..11775
                     /locus_tag="VW006_001640"
     CDS             11542..11775
                     /locus_tag="VW006_001640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380457.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         3494 a         2840 c         2042 g         3580 t
ORIGIN      
        1 gattatcaaa ttacttttat atcactaaga ctcaataaaa gataatgtct atttaacttt
       61 tatacgatta ctacctcctt tttagtttac acttcctaca tcataaataa acatactcaa
      121 ccctcacaat ctccaaataa ctcttctata tcattgaaaa ttgagtttca tcctctatac
      181 taatgtagga aacctattaa aacattcctt taatccaata taagaatacc ttgttccatt
      241 aatctctacc cgatattata gatagaacca gactcctata cttaaaagga caaataacgt
      301 tccaaccata ataaacctcc tttctttttg agaacgtttt ctttataggt ttagtatagc
      361 aaaaacatga aaaaaacaac ctgacttaga agtcaggttg ttgggaaatc tatctgtttt
      421 tctgctaaaa ttctctgaac taatacctga tcgtctagca attctgctag agaaatcgcc
      481 ttttcataga aactttctgc ctctaaaata ttcttgtcta caaataaagc atattttgcc
      541 tttagcaaaa atagatgagg tcggtaaaaa acatgtcttg ttttagcaat aattttctca
      601 aatgtttgaa tatatatcag actatcttct gtctgaattt ggataaatag gctcagaaga
      661 actttttcca tttgaactag gctagcctta tcttcatatt ctgaacatag caatactttt
      721 tgagctaact cctcaaaatg ttctttatct tctaatccca tagcacaaca aaagaaatac
      781 aattcaataa tcaacaaatc atttacagaa aaatatttct tttttaataa ctgctggaaa
      841 tattcttcca ctaaccctaa accgtaggtc acatctgaac taccataaat atcaaaacga
      901 gcttgtgtca catccacaat taactgctct tcctcaggca aattctcata atagttttca
      961 tatatttcct caagtatagc ttcttttcgt tcgattcttc ccttatcagc gtaggtttga
     1021 gaatgaatca gctcatcttt gagttttagg aatccttttg gaatctctat aaccttaaca
     1081 tccacaatat tctgtaatga tactccaagt ttatccgcaa tcaataataa tttaggaagt
     1141 gttgctaaag cttgcccatt ttcaatacgt gctagctgac gaatcgtcaa ctctgattca
     1201 tcaccacaaa tatctatctg agtttttcca caagatcgcc taatagtttt gattttataa
     1261 ccaatttcac gtttcaactc atccatagta atactcctta agttgattac aaagatttct
     1321 ttttcttcct aattcttttg tcaatcatat ataaaacgat aagcgaaaac agcggtagaa
     1381 caatataact cttttcactc attatcagct gaaatcccag ggttgctagt atcttaaaga
     1441 tgaaaatatt catcaaaaat agaaaaacaa atactaaaaa atattttaaa accttcttca
     1501 tttcttttct tcaactttcc taatttctac acctatttta tcgaaaatat atataaaact
     1561 ccaaataaac acttcgactg cacttttttt ggcaaaaaat agacagaaca aacatcctat
     1621 ctactaaaaa tatgatatga aaggaaattg attttttact gtcaccctag gcaagcttga
     1681 aacaaataaa tcattgatag taacacttac gacatcctat ttattacttc aatttctaat
     1741 gtctctgcta tttctaagct acctctggta tgatctattc ctagtcagaa tagataacac
     1801 cgttctaaat tacgattcat agttactcct tttttgactc gttactataa gactcctctt
     1861 tacatgcgca aaataatttt gattcagatt catcatttat agttttgatt attttttaac
     1921 agatttttct gttttcttct ccgaagactt taaaaatttt tttctaatta aatcatccat
     1981 ataaggacca aatataccaa ctactaaacc aataataaaa cttttaaaat ccataattac
     2041 caccaacatg tcgctgcata ggccacacct ccaagtatag ctcctcccgc agcaccagtt
     2101 gctgcacctt gccatgttct tgttttaatt cctagctgaa gacctcgtac tgctcctcct
     2161 ccaacacctg ctttggcaaa atctccccaa ttgcatccgc caccttcaac gcaagcaagc
     2221 atctcagtat ccataatttc aaattgtgac atcttttttg tattcataac gaatacctct
     2281 tttttatttt taatatttgt cttgttacaa acttgacaag tttagtataa cagtatctat
     2341 taattttttt catccaaatc ttgaattggc atcgaaacgt cttgaattag cttttttgtt
     2401 tcaaaatcat ctctattttt aaaaaagatg ttttctaatc acttttttac tattagactt
     2461 cctgcaaaac tagaatccta gttcatgatt gataatgcca gcaatcaaat tcattcgtaa
     2521 tccgaagtgt ttacgatgat ttcgataggt tgttgaaatc attttaaacg tttttacttt
     2581 ggcaaagatg ttctcaacct tgcttctctc cttggatagc gcatggttat aggctttatc
     2641 ttcagctgtt agcggcttga gtttgctgga tttacgtgga gtttgtgctt gaagatatat
     2701 cttcatgagc ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct
     2761 catttctaaa aaccatctac ttccgttgac ttggaactcc gattactatt ttccttgaat
     2821 gattttaacc acatctccta cgttttggag ttggtcaatt tcttcatcgc tgatttcgat
     2881 actaaattca tcttccagag tcaagataaa ctccatcaag tcaacagaat ccgcatccaa
     2941 atcgtctttc agactcaagg attctgtcac gacaaagtcc tctccctgtc gctcttggat
     3001 aatggtcaca atacggtcaa aaatttcttt ttctgtcatc tcttttattc tcctgaaaat
     3061 tcacgcgcag tctgggcaac cacgtctgtt tctagcatgg tacggatctg acgaatcgta
     3121 ctataaacag ccttggcatc gcttgagcca tgagtcttga caacaggtgc tttaacacca
     3181 aacaagaccg ctccaccaac atctgaataa ttgagctgtt ttttcaaacc actgaggctg
     3241 tccttgagaa ggagggcacc tagtttcgct cgaagaccac cacctgtaat agctgtcttg
     3301 agcaagccca tgattcccat agctgtccct tcgatggatt tgagcacagc gtttcccgtg
     3361 aaaccatctg ccacaacaac atctgcaacg ccattcatca aatcacgcgc ttccacgttt
     3421 ccgataaagt tcaaactttc atcagccgcc agtaattcat aagtttcctt acgaagcggg
     3481 tcgcccttgc tactctctgt tccgttgttg agcaaaccaa cgcgtggttg cgcaatgcca
     3541 cggacatttt tagcatagaa ggaacctaga accgcatatt gatggaggtg ctgggctgta
     3601 ttttctgcat tagcaccaag gtctagcatg tcaaaacctt ttccatcaac ggtaggcaag
     3661 gtagacatga gtccaggacg gtcgatattc ttgatacgac ccacgatgaa gaatcctgct
     3721 gccaacaagg cacctgtatt cccagccgaa aggacagcgt ctgcttcacc atctttgaca
     3781 gccttggctg ccaataccat actggcattt ttcttattcc taatagctct cgtaggttca
     3841 tcatccgaat caatcttctc atccgtatgg ataatgctga cgcgctctgt cgctgtcaga
     3901 tattgcttga ttttagcttc atctccgtaa agttgaacct cgatatctga aaagtcagat
     3961 agggcttgat tgacaccctc aacaatggcc tgaggtgcgt aatcgccccc catggcatct
     4021 actgcgattt ttttcatttc ttttcctctt ttagtaattg tccccagtct gctagggaat
     4081 caataaattt ctttgatttt aggtgaatcc caacgtactc ttcatatagt tgatccataa
     4141 attggcgtag ctcttgcttg attccaggct tgagcgaaat ggtctccaaa gtctcaaaat
     4201 caatagcttg aaattgattg agcagatagg ggatattggg attgagatga caacgtctct
     4261 tatcctcatg ataatgctct ggacagaggc aggctccata tttgaaagaa aagtcaaaag
     4321 cctgaccaac ccgatggcag aagacacact cattaaaatt gaggctgatt ccaaatcgag
     4381 tcaaaatttg aatttcaaaa atattggtca aaacctgata atccaagcct gcttccatca
     4441 actccaaagt cttttgcaaa aaagcaaaca agggagcatc ctgctgattg tcctgcaaac
     4501 tagcatctgc aagagctgcc acataggtcg cataggccat gacaaagagg tcactattaa
     4561 tcttgggaaa agtcatgacc tcatgatagt cttcgatgta actgagtccg tcatcattga
     4621 ttcgcaagag aaatcgtgcc agcaccaagg gctgaataac aggcgccagc ttagactgac
     4681 cagcgtgttt gacaaaaaac atgcgtttgc caacctgctc tgtaaaaatt ttgacgagct
     4741 tgtcatcctc acgaaaattg cgattgtaaa gcaccaagcc ttgactcgtg atagactgaa
     4801 tcatgcttct ctcatgtact cctcaagtcg tttcatggct tctttgatag tctccatgct
     4861 ggctgcataa gataggcgga cgtagccttc cccgtaacgt ccaaaggctg caccagggat
     4921 aaaggcaacg gccttcttct gagcaaaatc cttcagaaaa gcaaaggagt cttgattgta
     4981 gcccgctgga attttagcaa aaatatagaa ggcaccgtct ggtttgataa tctcaaaacc
     5041 aagagcagtc attttttcga tgatatagtc ccgacgttgg atatattcct tcttcatggg
     5101 ctccgcatcg tttttaccag ccgtcaaggc ttctactgca gcatgttgcg ccatggtatt
     5161 tgcggcagtg accaagtact ggtgactctt gattaactgg gctgtgaagg tcgcaggagc
     5221 gaaaatcagc cccaaacgcc aacctgtcat ggcatgcgat ttagacaaac cattgataat
     5281 aatagcctgg tctctcaaca tcgttcctag agacacatgg gcttcgcctg tgtaggtcaa
     5341 ttctgagtaa acctcatcac agacaacaaa aatttcgtac ttgcgtaaaa cagctgccaa
     5401 ggcctctaac tgctctcgac tgtaggtaat tcctgtcgga ttggctggat agttgagaat
     5461 aaccgccttg agcttatcac cctgctccaa aatggccttc tccaacatct caggagtcaa
     5521 gacaaaacca ttttcagtcg tatcaatctc aacaatttct gccccaacta agttaacaat
     5581 cggttcatag cctggataag caggagctgg caaaagtacc ttgtctccct cttccaaaat
     5641 agccgtcaaa gtcgcagata aagcctctgt cgccccaatt gtaaccaaga tttcattttc
     5701 aggagcatag tccagttggt acttttcctt aacaaagtca ctggctgcct gacgtagagt
     5761 cagcagacca ctcatccctg tatagtagga ttggttctga tcaatcgctc gcttggccgc
     5821 ctccttgaca tggtctggcg ttgtaaaatc aggttccccc aaggtcaaac gcaagacccc
     5881 aggaatctcc gaaataacct ggtcaaactg acgaatcaac gaaacttgaa ttttatctaa
     5941 ctgtttatta aagcgcttag ttaagtccat agatacctcc tactttcaaa acgtatctct
     6001 attatactac aaaagccccc cgaccgcaaa aatacattag agaatagctt tccactttct
     6061 attttacttt tcctgtttat aggtctataa tggtaataga ttttatttgg aggtttttat
     6121 gtcatttcta tcaaaaaatg gagcaggtat cttggcctgc cttctcattt ccatcctatc
     6181 ttggtactta ggaggattct tccctgtgat tggcgcgccc gtttttgcca ttttcatagg
     6241 catgctccta catccctttc tctcgtccta taaacaactg gatgctggtt tgacctttag
     6301 ttccaagaag ttgctccaat atgccgttgt cttgcttggt tttggtctca atatctcgca
     6361 ggtcttcgca gttggccaat cttcactccc tgtcatcctg tccactatct caatagctct
     6421 gattattgcc tacctcttcc agcgtttctt tgcactggat acaaaactgg ctaccttggt
     6481 tggagtaggt tcttctatct gtgggggttc tgccattgca gcgacagcgc ctgttatcca
     6541 tgctaaggaa aaggaagtag cccaagccat ttccgttatc tttttcttca atgtcttggc
     6601 tgcgctcatc tttccaaccc tcggcacctg gcttcatcta tccaatgaag gcttcgccct
     6661 ctttgcaggg actgcggtca acgacacttc ctctgtaacg gctgccgcca gcgcttggga
     6721 cagtctttac caaagcaata ccctcgagtc tgcaaccatt gttaaactca cacgtacttt
     6781 ggccattatc cctatcacgc tctttctatc ctactggcaa agtcgccaac aagaaaacaa
     6841 gcaaagccta caactgaaaa aagtcttccc actttttatc ctttacttta tccttgcctc
     6901 tctcctcact acactactca cctctctagg tgtgtccagt agtttcttta cccctctcaa
     6961 acaactctct aaattcctta ttgtcatggc catgagtgct atcggtctca aaaccaatct
     7021 ggtcgctatg gtcaaatcca gtggaaaatc cattgttctt ggagccatct gctggattgc
     7081 catcatcctc accactcttg gtatgcagac ccttatcggc attttctaac actcttcgaa
     7141 aatctcttca aaccacgtca acgtcgcctt gccgtaggta tatgttactg acttcgtcag
     7201 ttctatctac aacctcaaaa cggtgttttg agctgacttc gtcagttcta tctacaacct
     7261 caaaacggtg ttttgagctg acttcgtcag ttctatctac aacctcaaaa cggtgttttg
     7321 agctgacttc gtcagttcta tctgcaacct caaaacggtg ttttgagcag cccgcggcta
     7381 gtttcctagt ttgctagtgt gctctttgat tttcattgag tataacacaa aaggtagccc
     7441 atcagctacc tttttcttat gcttcctcaa tcaagcgagt atgttctctc ttgatacagc
     7501 gattcatcac gatatcatca catccaccat cacgcaaaat ctctttcgct tctaaacttt
     7561 caagtcctag ctgtgcccaa aaaatcttgg catcagcttt gagaaaatca cgcgccacat
     7621 cgggcagaaa ttcactgcga cgataaacat tgacaatatc tacaggaaaa ggaatttcag
     7681 cgaggctagc ataagccttt tcacccaaga tttcgccacc tgccgccttg ggattgactg
     7741 ggatgatttt atagccccga gcctgcattt cctttgttac tcgattgctg gttgtttctt
     7801 cacggtcaga caaacccacc acagcaaggg ttttactcgt tgcgagatac tgacgaatca
     7861 cgccatcact tggattgata aattcttgac tcatagaaat cctccttttt catcagtata
     7921 gcacattttg aaaaggtttg cagaattata ctacaaaaaa ggaggactag cccccttttt
     7981 atttagcctc gtaccaggtt gccccttcat tctcatctgc gataagagga acactgagtt
     8041 gaatggcttc ttccatggtt tgtttcacca attttttcat ctctaccaat tcagatttag
     8101 gcacttcaag gacgatttca tcgtgcactt gtaacagcat cttagtctga taaccacctg
     8161 caaccaaggc tttatccagc tgaatcatgg caatcttgag aatatctgct gccgaaccct
     8221 ggataggtga gttgatagca gttcgctccg caaaaccacg aatattgaag ttgcgcgaat
     8281 tgatatctgg caactcacgg cgacgcttaa agagggtctc tacatagccc ttatcacgcg
     8341 cctcccgcac cacttcatcc atgtagtttt taatatctgg aaaacgttca aagtaggtat
     8401 caatgtaggc tttggcttcc ttacgactaa ttcccaaatt attagacaag ccaaagtctg
     8461 aaatcccata aaccactcca aagttaactg ccttggcatt gcgacggtca tttgcagtca
     8521 cattatcagg acgctcaatg ccaaagaccc gcatggctgt cgaagtatgg atatctgccc
     8581 cctcttggaa ggccttaatc aagtgctcat ccttagaaat atgcgccaaa acgcgcaatt
     8641 caatctgtga atagtcagag ctgagtagca cactatcctc ccactctggc acaaaagcct
     8701 tccgaatcaa gcgcccttgt tccaatcggg caggaatatt ttgcaagttt ggatccacac
     8761 tagacaaacg cccggtctgg gtcaaatcct gcatatagcg agtatgaatc tttccatcag
     8821 ccaaaatcca gtcctgcaag ccaattacat aagtagattg aatcttagca atttgacggt
     8881 aatccaggat tttcttaaca atcggagcaa taggagcgag acgctccaac acatccactg
     8941 ctgtcgaata acctgtcttg gttttcttag tgtattctag aggaagtccc aatttctcaa
     9001 agagaagcac gcccaactgc ttaggcgagt tgacattaaa ctcctcacca gccagctcgt
     9061 aaatctcttg agtcagtttt tcaatgacaa gctcattttc agcctgcatc tcaagcaagg
     9121 tctctttctt gaccacaatc ccagcaattt ccatcttggc aagaacaaaa gccagaggtt
     9181 gctccatatc ataaagaagc tctaattgcc cattttcgct gagtttttca agtaaaatag
     9241 gctctgtttc taccaaaaca gcaagtttac aagctaagtg ttccaagaat ttctcacgtt
     9301 caggaatggc ctttttaaca cccttaccgt agaaagtttc atcatcaacc aagtaagtct
     9361 gaccataaag actagcgatg gtcgcaattt cattgtcctc cacagtcgaa aggaggtatt
     9421 tagccaaacg gatgtcaaaa gcaggcgcct gcaaatccac accaaaacgt tgcaaaagaa
     9481 ctttaacctt cttaaagtca taaactctca gagatgtttt ttctaagaaa tccttgaaaa
     9541 tcgggtcttg caacagctca agcttgtctg tggcatagag cttatcctca caagaccagg
     9601 caaatccaac caaattatcc gtatggtaat tctcaccaaa aagctcaaag tggaagatag
     9661 actcttcact cagcatatct tgactgattt ggtcaacaat agtaaaatcc aaacccttag
     9721 ccacatcagc tgacgacaca tttaaagcct gcttgagctg tttgaatccc atctcatcgt
     9781 aaaatttccc aagattttca acatctggac cactatagac caagtcctct aaaccaatcg
     9841 caatcggtgc cttggtatca atggtcgcta gtgttttaga caaaaaggcc tgttccttgt
     9901 cattgatgag attttccttc atcttagaag tcttcattcc atcaatattt tcataaatcc
     9961 cctcaagcga accatactcc agcaagagct taatacccgt cttttcaccg actttggtca
    10021 ccccagggat attatccgac ttatcaccca tgagcgcctt gagatcgata aactgagctg
    10081 gtgtgaggcc catttcttcc atgaggtaat ctggcgtaaa ggcctcaaac tcagccacac
    10141 ctttcttgga aatttcaacc accgtatgct catccgtcag ctgaatcaaa tccttgtccc
    10201 cactgacaat ggtaatatca aaaccatcct gctctgctag cttatccagc gtcccaatga
    10261 tgtcatccgc ctcatactga gccagctcat agtgacgaat ccccatatga tccagcaact
    10321 cacgaatgaa aggaaattgc tcacgaaact catcaggagt cttggcccga ccacccttat
    10381 agtccgcata catctctgtc cggaaggtcg tctttcccgc atcaaaagcc accaaaatat
    10441 gactcggctc aacccgctcc aacaaatgac tcaacatcaa ctgaaaacca taaatcgcat
    10501 tggtatgcaa accagccgca ttcttaaaac ggtccaactg ctgatacagc gcaaaaaacg
    10561 cccgaaaagc tacagaagac ccatcaatca ataataattt tttcttatcc atacacccat
    10621 tataaaggaa agaatcaaaa aataccattg ggaagagcta gagcaagtat ttttcaaact
    10681 ttttccgaat aaatagatag agccagagaa tttagtaaac ctagatttaa aaatgtgcta
    10741 taatataata tattgaatct ataatagtac accttgactg ctaaaatatt tctataaatt
    10801 aatttgactt tcctgataga gttattcaca tcttatttca actcactata gaaggaggaa
    10861 taggaggatt ctcagacatc cgggcatcag cccaactaat gatttgattg ctaagaaaat
    10921 attcagcaat ccagaaatca cttgtcaatt tattcgcgat atgctggact tgccagcaaa
    10981 aaatgtgacc attttggagg gaagcgatat tcacgtatta ctctccatgc cttactcggt
    11041 gcaggatttt tataccagta tagacgtctt ggcggagttg gataacggta ctcaagtaat
    11101 tattgagatt caagtccatc atcagaattt tttcatcaat cacttgtgga cttacctgtg
    11161 cagtcaggtt aatcaaaatc ttgaaaaaat tcgtcagcga gaaggtgata ctcactagag
    11221 ctacaaacac atcgctcctg tttacgccat tgctatcgtg gatagtaatt atttctcaga
    11281 tgacctggct tttcatagct ttagtatgcg cgaagacaca acaggtgagg tattggcgat
    11341 taccaacaat ggacaggaaa accatctggt taagatggca ttcttggaat taaaaaatac
    11401 agagaaacca gcaaagacaa ggttcgcaag ccatggttgg agtttttcgg caacaagccc
    11461 tttacccagc aaccgcaacg agccattagc caagcaaatc aactgctgga ctacaagagc
    11521 tggtccgagg aggacaggaa aatgtttagt caactacata tgcgagaaga acaagtcttg
    11581 ttagcacagg actatgcctt ggaaactgct agggctgaag gccttgaaca aggactagag
    11641 cgtgggaaag ttgaaggaag ggcagaaagg aaactttttg ccttcctaga catagtacgc
    11701 caaggtcttc tgacttctga ggttgccagc cagcaattag gtatgtcagt atctgaattt
    11761 gaggcactgt tgtaaaatgg ctccataata tccatagtgg gtaaatcccc tatggatatt
    11821 atggagccta ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa
    11881 caactcatta gaaagaatca tatggaacaa ttacatttta tcacaaaatt actagacatt
    11941 aaagacccta atgtcc
//
LOCUS       DAVKFH010000055        11924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKFH010000055 DAVKFH010000000
VERSION     DAVKFH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.055"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            153..806
                     /locus_tag="VW006_001641"
     CDS             153..806
                     /locus_tag="VW006_001641"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9380458.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            816..1280
                     /locus_tag="VW006_001642"
     CDS             816..1280
                     /locus_tag="VW006_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9380459.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1293..2561
                     /locus_tag="VW006_001643"
     CDS             1293..2561
                     /locus_tag="VW006_001643"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9380460.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKFNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2575..2835
                     /locus_tag="VW006_001644"
     CDS             2575..2835
                     /locus_tag="VW006_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380461.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2846..4207
                     /gene="pdrM"
                     /locus_tag="VW006_001645"
     CDS             2846..4207
                     /gene="pdrM"
                     /locus_tag="VW006_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9380462.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4361..5329
                     /locus_tag="VW006_001646"
     CDS             4361..5329
                     /locus_tag="VW006_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9380463.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5345..6337
                     /locus_tag="VW006_001647"
     CDS             5345..6337
                     /locus_tag="VW006_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9380464.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6555..7598
                     /locus_tag="VW006_001648"
     CDS             6555..7598
                     /locus_tag="VW006_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9380465.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7662..9347
                     /gene="lpdA"
                     /locus_tag="VW006_001649"
     CDS             7662..9347
                     /gene="lpdA"
                     /locus_tag="VW006_001649"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9380466.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9411..10400
                     /locus_tag="VW006_001650"
     CDS             9411..10400
                     /locus_tag="VW006_001650"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9380467.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10473..11543)
                     /gene="xerS"
                     /locus_tag="VW006_001651"
     CDS             complement(10473..11543)
                     /gene="xerS"
                     /locus_tag="VW006_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9380468.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3539 a         2223 c         2665 g         3497 t
ORIGIN      
        1 aattataaaa aatagccagt ctttataaac tgtattcacg tattcaaatc gtatatatag
       61 tcgatttatt aaatctttag ataaactgta aaaagtggtt tccatagcca ctttttgcta
      121 taatagaggc agtaaaaatg aaggagtcgg ctatggaaca ctcgtcttgg catgctttga
      181 ttaaggcgca attacctgag ggttatttcg ggaaaatcaa tcagtttatg gagcaggtct
      241 attctcaggg gattatttat ccacccaagg aaaaggtttt tcaggctctc ttgacaacac
      301 tgcttgaaga agttaaggtg gtaattctag ggcaagaccc ctatcacgga ccaggtcaag
      361 cgcagggctt gagtttttct gtacctgact ctatcccagc tccaccatcc ttgcaaaata
      421 tcttgaaaga attgtcagat gatatcggag ttaagaaatc tcatgatttg acagcttggg
      481 ctgagcaagg agtcttgctt cttaatgctt gtttgactgt tcctgctgga caggccaatg
      541 gtcatgctgg tcaaatctgg gagcctttta ctgatgctgt gattcaggtg gtcaatcatc
      601 tagatagacc agtcgttttt gtactctggg gagcttatgc acgtaagaag aaggcattag
      661 ttaccaatcc tcatcacttg attatcgaat cagcccatcc aagtcctttg tcggtttata
      721 gaggattttg gggttccaag cccttttcca aggccaatgc attcttaaaa gagacaggac
      781 aagagccaat cgattggctt agataaggag aaaatatgcc tcagttagcg acgatttgct
      841 acattgataa tgggaaagaa ctgctcatgc tccatcgtaa taagaaaccc aatgatgtcc
      901 atgaagggaa atggattggt gtgggtggta agctagagag aggagagacg ccccaggaat
      961 gcgcggcgcg tgaaatcctt gaagaaacag ggctcaaagc caagccagtt ctaaaaggtg
     1021 tcatcacttt tcctgaattt acaccagatt tagactggta cacctatgtt tttaaggtga
     1081 cggagtttga gggcgacttg attgactgca atgaggggac gctagaatgg gttccctatg
     1141 atgaggtttt gagcaagccg acttgggaag gtgaccacac ctttgttgag tggcttttag
     1201 aggataaacc cttcttttca gccaagtttg tttatgatgg ggataaattg ttggataccc
     1261 aagttgattt ctatgaataa aggagaaagc agatgctact aatcaaaaat ggtcgtgtaa
     1321 tggatcccaa gtctggtttg gatcaagttt gtgatgtctt agttcaagat gggaaaatta
     1381 tcaaaattgc gcctgagatc acggaagaag gagcagaaac gattgatgct actggtcttg
     1441 tggttgctcc tggtttggtc gatattcatg tgcatttccg tgaacctggt caaacacata
     1501 aagaagacat tcatactggt gccctggcag ccgctgcagg tggttttact actgtcgtca
     1561 tgatggctaa taccagtcca accatttcag acgtggagac tttgcaagca gttctccagt
     1621 cagctgccaa agagaagatt aatgtcaaga cagttgcgac cattactaaa aactttaatg
     1681 gtaaaaactt gactgacttt aaggcgctct tagaagctgg tgcggctggt ttctctgatg
     1741 acggtattcc gcttgagagc agtaagattg tcaaggaagc catggaggaa gccaaaaaat
     1801 tcaatacctt tatcagcctt catgaggaag atccaggttt gaacggtgtt cttggcttta
     1861 atgaaaatat tgctagagaa catttccata tctgcggtgc tactggagtg gctgagtacg
     1921 ctatgatggc gcgtgatgtc atgattgcct atgcaactaa agcccatgtt cacatccagc
     1981 atttgtctaa ggaagaaagt gttaaagtag tggagtttgc tcaggggtta ggtgcagaag
     2041 tcacagcaga agtagcgcca cagcatttct ctaagacaga agcacttctt ttaacacaag
     2101 gtagcaatgc taagatgaat ccaccgcttc gtttggaatc agaccgtcgt gccgttatcg
     2161 aaggtctcaa atcaggtgtc atcacagtta ttgcgactga ccacgcgcct catcatgtag
     2221 atgaaaaaaa tgttgaggat attaccaaag cgccatctgg tatgactggc ttagaaacat
     2281 ccctgtctct cagcttgacc tatttagtag aagctggtga gttgagcttg atggaattac
     2341 ttgaaaaaat gacatacaac ccagccaagc tttacaactt tgaagcaggt tacttggctg
     2401 agaatggtcc agcagatatc actatttttg atgccaaggc tgaccgcttt gtggactccc
     2461 attttgcttc caaagcagct aattcaccat tcatcggtga aaccttaaaa gggcaggtta
     2521 aatataccat ctgtaaggga caaatcgtct atcaagcttg ataggagtta gaatatgaat
     2581 tattttcgaa aacgtaggga gagacaagcg aaatctaata gtggaattta ctgtcccgca
     2641 gctaatcgtt ccaaaattca atatgaaact aaagaaaaag ctgaccatgc ggttcaatat
     2701 gataacggag gtttagttag aagctattat tgtcgtacat gtgcgtgttg gcatactact
     2761 tcaaaatcta ataaacctcc gctaagtttg aaaaactatg gtctgaaact ctttggttta
     2821 gataaatcag aatagaaaga gaagtatgta taagacaaag tgtttacgag agaagttagt
     2881 attattttta aaaattttct tcccaatcct gatctaccaa tttgccaatt attctgcctc
     2941 ttttgttgat actgcaatga caggtcaata caacactatg gacttggctg gtgtatctat
     3001 ggcaaccagt atctggaatc ctttctttac atttctaaca gggattgtgt cagccttggt
     3061 gcctatcatt ggtcaccatc ttggtcgagg caaaaaggaa gaagttgcgt ctgattttta
     3121 ccaatttatt tatttggcct tgggcctatc tgtggtcttg ctggggatgg tacttttctt
     3181 ggcaccaata atcttgaatc atattgggtt agaagcagca gtagcggcag tagcggttcg
     3241 ctatctttgg tttttatcta tcgggattat ccccttgttg ctctttagcg tcattcgttc
     3301 cttgctggat tcgctgggct tgaccaaact gtccatgtac ctcatgcttt tgttactccc
     3361 tctcaatagc ggatttaact atctcttgat ttacggtgcc tttggtgttc cagaactggg
     3421 aggggctggt gctggtttag gaacatcctt ggcctactgg gtcttgcttg ggatttctgt
     3481 tctggtttta tttaaacagg agaagctcaa agccttacac cttgagaaac gaattccact
     3541 taatatggat aacattaagg aaggagttcg tttaggtctg cctattgggg gaactgtctt
     3601 cgcggaagtg gctatctttt cagtggttgg cttgattatg gctaagtttt cgtccttgat
     3661 tatagctagt caccagtcag ctatgaactt ttcaagtctt atgtacgcct ttcctatgag
     3721 tatctcatcg gctatggcta ttgtcgtttc ctatgaagtg ggagccaagc gatttgatga
     3781 tgcgaaaacc tatattggtc taggaagatg gactgctctc atttttgcgg ccttcacctt
     3841 aaccttcctt tacattttta ggggaaatgt ggccagtctt tatggtaacg acccaaaatt
     3901 tatcgatttg acagtgcgtt ttttaactta tagtcttttc ttccagttag cagatacctt
     3961 tgcggcgccg cttcagggaa ttttgcgggg gtataaggat acagttattc ctttttacct
     4021 tggtttgctt ggttattggg gcgtagcaat ccctgtggct acgctatttg attccctaac
     4081 agattttgga gcttattctt actggatcgg cttgattatt agtttgattg tgagcggggc
     4141 gctctaccgt tggcgtttaa ctgtgattat gaagagattt gaatctttag caaaatccaa
     4201 atgctaaaaa gtttgtgaaa aaatattctt gacaagaaaa tcccttgttt tcacggggtt
     4261 ttctttttta ttttgtgaaa tttcttttag aaaaactttg acagtatttg ccaaaagcgg
     4321 taaaatagta aggtaagaag aaagttagaa aggcaagaag atgtcaactt tagataaaaa
     4381 tcttttgcta gaaatgttcc gtaagatgga agaaatccgt cgcatggact taaaaattgc
     4441 acaattagta aagaaaggga aagtgccagg aatgacgcac ttttctgttg gtgaagaggc
     4501 agctaacgtg ggggctatgt tagctctcaa tccagatgat ctgattacct caaaccaccg
     4561 tggacacggg caagctattg ctaaagggat tgacctcaac ggaatgatgg ctgaaatcct
     4621 tggtaaatac actggaacct gtaaagggaa aggtggatct atgcatatcg ctgaccttga
     4681 tgctgggaac cttggtgcca atggtatcgt aggtggtggt atggggatcg ctgtcggtgc
     4741 agccctcagt cagcaaatgc aaaataccgg taaaatcgtt gtctgcttct ttggagatgg
     4801 tgcgaccaat gaaggtgttt tccacgaagc agtgaacatg gcttctatct ggaacctgcc
     4861 agtcattttc tattgcatta acaacggtta cggtatctct gcggatatca agaaaatgac
     4921 caatatagaa catatccatc aacgtagcgc cgcttatgga attcctggaa tgttcatcga
     4981 agacggtaac aatgtcatcg atgtctatga aggatttcag aaagctgtgg accatgttcg
     5041 cagtggcaat ggtccagtct tgattgaaag tgtaacttat cgctggctcg gtcactcatc
     5101 atctgaccct ggtaaatatc gtacgcgtga agaagtggaa ttgtggaaac aaaaagatcc
     5161 aatcgaaaac ctccgcaatt accttattga aaataacatt gcaagtgccg aagaattgga
     5221 agaaatccaa gcgcaagtaa aggaagcagt agaagcttct gttaaatttg cagaggaaag
     5281 cccattccca ccgcttgaat cagcctttga agatatttac gcagactaag gagaaagaga
     5341 taaaatggaa acaaaaacaa tgtccttccg tgacaccatt atccttgcta tgtctgagga
     5401 aatgcgtcgc gatgaaaatg tgttcttgat gggagaagac gtcggtgtct tcggaggaga
     5461 cttcggtact tctgttggaa tgcttgaaga atttggtcca gaacgtgtcc gtgactgtcc
     5521 gatttctgaa gctgccatct ctggagcagc agcaggagca gccatgacag gacttcgtcc
     5581 aatcgtcgat atgaccttca tggacttctc tgttattgcc atggacaata tcgtcaacca
     5641 agctgctaaa acacgttaca tgtttggtgg taaaggtcag gttccaatga ctgttcgttg
     5701 tgcagctggt aacggagttg gctctgccgc ccagcactca caatctctag agtcttggtt
     5761 tactcacatt ccaggactta aggttgtggc acctggaaca cctgcggaca tgaaaggcct
     5821 gcttaagtct tctatccgtg ataacaactc agttattata cttgagtaca agtcagaatt
     5881 taaccaaaaa ggggaagtgc cagttgatcc agactacaca attccacttg gggttggtga
     5941 aatcaaacgc caaggaacgg atgtaacagt tgttacttat ggaaaaatgc ttcgccgtgt
     6001 ggttcaagct gctgaagaat tagcagaaga aggaatttca gttgaaattg ttgacccacg
     6061 tacccttgtt ccgcttgata aggatatcat cataaactca gtgaagaaaa ctggtaaggt
     6121 tgttctggtc aacgatgccc acaaaacaag cggctatatc ggtgaaattt cagctattat
     6181 ttcagaatca gaagcatttg actatctaga tgcaccaatc cgccgttgtg caggagaaga
     6241 tgtgccgatg ccttatgcac aaaacctaga aaatgcaatg attccaacag ttgaaagtat
     6301 caaagatgca atccgaaaaa cttataacaa agaatagggt tacataagat aagttatgta
     6361 aataaaataa agctgacgag aaactttgta tcttttaggt gcagagctct tcgtcattaa
     6421 tatcttagat tagagcacgg gctaaaagat cggaaaaaag ataaatctcc ttggcttcat
     6481 cgaagccatc gtcgatttcc tatttttcag tcgcttttga cgccctttgt atcataaata
     6541 gaattggaga ggtcatggct gatgacaagc taagagcgac tcctgcagct agaaagttag
     6601 cggatgattt agggataaac ctctacgacg tttctggctc aggtgcaaac ggtcgtgtcc
     6661 acaaagaaga cgtggaaact tataaagaca caaacgtggt tcgcatttcg ccacttgcaa
     6721 aacgaattgc cctcgaacat aacattgctt ggcaggaaat ccaaggaacc ggtcatcgtg
     6781 gtaaaatcat gaagaaggat gttttggccc tgcttcctga aaatatcgaa aacgatagca
     6841 tcaaatctcc tgctcagatt gaaaaagtgg aagaagtccc tgataacgta acaccatatg
     6901 gtaaaatcga gcgtattcca atgacaccaa tgcgtaaggt tattgcccaa cgcatggttg
     6961 aatcttactt gactgcgcca accttcaccc tcaactatga agttgatatg actgaaatgt
     7021 tggctcttcg taagaaggtt cttgagccaa tcatggaagc aactgggaag aagactactg
     7081 taacagacct tctttcgctt gcagttgtta agactcttat gaaacaccca tacatcaacg
     7141 cttcattgac agaagatggc aagactatta tcactcacaa ctatgtcaat cttgccatgg
     7201 cagttgggat ggataatgga ttgatgacac ctgttgttta caatgctgag aagatgagtc
     7261 tttcagaact ggttgtagcc tttaaggatg ttattggccg taccttggat ggtaaattgg
     7321 ctccaagtga gttacaaaat tcaacattca caatcagtaa tttgggaatg tttggtgttc
     7381 agtcctttgg tccgattatt aaccaaccca actcagctat ccttggtgtc agttcgacaa
     7441 tcgagaagcc agttgtcgtc aatggtgaaa ttgtgattcg cccaatcatg agtttaggat
     7501 taaccattga ccaccgtgtc gtagatggta tggctggtgc taagtttatg aaggacttga
     7561 aagagttgat tgaaacgcca atctcaatgt taatttaaga gcaagagtat tcatttttaa
     7621 gatatagata aaggaaggaa gacatggcct tagaagtaat tatgccaaaa gccggcgtgg
     7681 atatgacaga aggacaaatc gtccaatgga ataaaaaagt cggagaattt gtaaaagaag
     7741 gagaaatcct tttggaaatc atgactgata aagtcagcat ggaattggaa gccgaagaag
     7801 atgggtactt gattgccatt ctcaaaggag atggtgaaac tgtccctgta acggaagtta
     7861 tcggttacct tggtgaagaa agggaaaaca tcccaacagc tggagcagca tcgccagaag
     7921 ctagctctgt acctgtagca agtacctcaa acgatgatga taagagcgat gatgcttttg
     7981 atatcgttgt gattggtgga ggtcctgctg gttatgttgc agccattaaa gctgcccaat
     8041 tcggcggtaa ggttgccctt gttgagaaat ctgaacttgg tggaacctgc ttaaaccgtg
     8101 gatgtattcc aaccaagacc taccttcata acgctgaaat tattgaaaat atcggtcatg
     8161 ctgcaaatcg tggtatcgtg attgaaaatc ctaacttcac tgttgatatg gaaaaacttt
     8221 tagaaactaa atctaaagtt gttaatactc ttgttggtgg tgttgcagga cttcttcgta
     8281 gctacggagt tactgttcat aaaggaattg gtacaatcac taaagacaag aacgtcttgg
     8341 taaatggttc tgaattgctt gaaaccaaga aaatcattct tgctggtggt tcaaaagtca
     8401 gcaagatcaa cgtccctggt atggaatctc cacttgtcat gactagtgat gacattcttg
     8461 aaatgaacga agtgccagaa agccttgtta tcatcggtgg tggagttgtc ggtatcgaac
     8521 tcggtcaggc cttcatgaca tttggttcaa aagtgactgt tatcgaaatg atggaccgta
     8581 tagttccagc tatggatgtg gaagtttcta agaaccttcg cttgattctt gagcgtaaag
     8641 gaatgaccat cttgactggt actaaactgc aagaaatcat tgaggaaaat ggtcaacttc
     8701 gtatcaaggt tgaaggaaaa gacgatatca tcgcaagcaa agctcttctt tcaattggtc
     8761 gtatgccaga ccttgaaggt attggagagg ttgagtttga attggatcgt ggttgtatca
     8821 aggtcaacga atacatggaa acttcagttc caggcattta tgcaccaggt gacatcaacg
     8881 gtactaagat gttggctcac gcagctttcc gcatgggtga agtttccgct gaaaatgccc
     8941 ttaaaggaaa tcatgcagtt gccaaattga atttgactcc tgcagccatc tacactctcc
     9001 ctgaagtagc agcagtaggt ttgacagaag aacaagcccg tgagaaatac gatgttgcca
     9061 tcggtaagtt taactttgct gctaacggtc gtgctattgc atctgacgca gctcaaggtt
     9121 tcgtaaaagt tatcgctgat aagaaatacg gagaaatcct tggtgtacac attattggtc
     9181 ctgcagccgc agaattaata aacgaagcat caagcatcat cgaaatggaa atcactgttg
     9241 aggaaatgct gaagaccatc cacgggcacc caacttactc tgaagtgatg tacgaagcgt
     9301 ttgcagatgt tctaggaatg gccatccatt cacctaagaa aaaataaaac gaagatagat
     9361 tagactggaa ggttatttcc agtttctatc atataaagta aagggatatc atgaaataca
     9421 ttatcaatca ttcaaatgac actgctttta atattgcctt ggaagaatac gcctttaaac
     9481 accttttgga tgaggatcaa atcttcctac tttggattaa caagccatct atcattgttg
     9541 gtcgtcacca gaacactatc gaagaaatca accgtgatta tgttcgcgaa aatggtattg
     9601 aggtagttcg ccgtattagt ggtggtggag ctgtttacca cgatttaaat aacctcaact
     9661 acacgatcat ctccaaagaa gatgaaaata aggcatttga cttcaagagc ttctcaactc
     9721 cggttatcaa taccctggct caacttgggg ttaaagctga gttcacaggt cgtaatgacc
     9781 ttgagattga tggtaagaag ttctgtggca atgcccaagc ctatatcaac ggccgtatca
     9841 tgcaccacgg ttgcttgctc tttgacgttg atttgtcagt cctcgcaaat gcccttaagg
     9901 tttcaaaaga taaatttgaa tcaaaaggtg tgaaatccgt ccgtgcccgt gtaaccaata
     9961 ttatcaatga attaccaaaa aaaatcacag tcgaaaaatt ccgtgattta ctcttggaat
    10021 acatgaaaaa agagtaccca gagatgactg aatacgtctt ttcagaagaa gaattggccg
    10081 aaatcaatcg catcaaggat actaagtttg gaacttggga ctggaactat ggtaaatcac
    10141 ctgaatttaa cgtccgtcgt ggaatcaaat tcactagtgg taaggttgaa gtctttgcta
    10201 acgttactga atcaaaaatc caagacatca agatttatgg tgacttcttt ggtattgaag
    10261 acgttgctgc agtagaagat gtccttcgtg gggtgaaata cgaacgcgaa gatgttctta
    10321 aggcgctaaa aaccattgat atcacacgct acttcgctgg tattagccgt gaagaaatcg
    10381 ctgaagcggt agttggataa atcaaaaaga agttggccaa ctaaccaact tctttttttg
    10441 atatttattt acataattta aaatacgtaa aatcataaac tatccagagc attcttttgt
    10501 tcatcattaa caatatgggt atagaggtca gtgacttgtg tgctggcatg tcctagttgg
    10561 tgactgacta aaacttgtga tttagtagca tcatagagcc tagttgctag tgtatggcgc
    10621 agtttatggg gtgttacacg tactttaaaa tcctctgagt atttagcaac cattttctca
    10681 acgctagaag catcgatacg attaggaaca cctctgtaga gagttaaaaa aagggctgta
    10741 tctgtttttt ccgttttata gcgttgattc cgaatggcca gataattctc taaataaggt
    10801 ttagcaaaag cagcgacatt gactgagtca cgtttgccac cttttcgagt aacatcaata
    10861 accatcattt ttagattgag atctcttaga tctagattaa cagcttcaga taagcgaaca
    10921 ccagatgcca agagaagggc aataatggct aaatctcgtt ctttattttt gttgaatgat
    10981 gagagagctc gatttgaaag ctgttgtggg tgctcttgat cgatataagt tagaaaacct
    11041 tctgtttcat cacctagaaa gagtttttgc ttgatatttt cagctctggc agcaagggtt
    11101 tctttctttt tcttggttga aacttttttc attacattac gatagaaata aggttccccc
    11161 tgatcgtttt caacctcctc ggttagatac ttgtaaagac tagaaagtgc tgataaggtt
    11221 cgattgatag ttgtctgtga aacaccttgt tttgttgtat tagcattcag caagggacgt
    11281 tcacgtagat aaaggataaa ggattccatg tctttcttag acatattttc caaaactgat
    11341 aaaggaatat cggatatttt atcagcgttt gaaataccag actccaaaac ccagctgaaa
    11401 aatcggtcat attccttaag gtattcgtac agggttgtaa aactgtaggg cacagccagt
    11461 ttagattggt agtattccag aacataccag ggcatgagtt gttttagttt gtcgattcgt
    11521 tccagtaaaa tctcacgttt catgtatttt ctccataatt aagtatacta gtagtatagc
    11581 atagacttgt atatttttca agaaaattat agtttttcag aaagatgcta taggagtttt
    11641 tcaaagtgat aaaaagactt agacctacaa acaaaacaga ataaggaaaa taataaaaat
    11701 ctcacccact aaacttctgt taatactaca taactgaaga gctatataag gagctatgag
    11761 atacaaaaca cattgtataa tagattgaaa ctagaatagt acatctggac ttctaaaaca
    11821 ttgctagaaa ttgatttgat tgtcctgatt gttttatcct atttctattt catttctcta
    11881 tataattgct attgttttct ttttcatgat tttaatctta ctgt
//
LOCUS       DAVKFH010000056        11866 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKFH010000056 DAVKFH010000000
VERSION     DAVKFH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.056"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..424)
                     /locus_tag="VW006_001652"
     CDS             complement(<1..424)
                     /locus_tag="VW006_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380469.1"
                     /translation="MRGFNNKIKSVYQELTNSKEKFGSFHKTLIHLHTPVSYDYKLFS
                     NWTATKYRKITEDELYDIFFENKKIKVDKTIFFSNFDKVVFSSSKEYISFLMLAEAII
                     KNGIEIVVVTDHNTTKGIKKLQMAVSIIMKNYPIYDIHP"
     gene            complement(452..1213)
                     /locus_tag="VW006_001653"
     CDS             complement(452..1213)
                     /locus_tag="VW006_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9380470.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELFFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(1213..1689)
                     /locus_tag="VW006_001654"
     CDS             complement(1213..1689)
                     /locus_tag="VW006_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380471.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(2134..2415)
                     /locus_tag="VW006_001655"
     CDS             complement(2134..2415)
                     /locus_tag="VW006_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380472.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            complement(2461..2778)
                     /locus_tag="VW006_001656"
     CDS             complement(2461..2778)
                     /locus_tag="VW006_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380473.1"
                     /translation="MNCRGHETRQRIVRDFEVQPKVHIKLLANQQKHSDAVATIEDEY
                     YVFIAESKIDGKKEVIQCCMGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGS
                     GNL"
     gene            complement(2864..4603)
                     /locus_tag="VW006_001657"
     CDS             complement(2864..4603)
                     /locus_tag="VW006_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU9380474.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESASNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELD
                     NQLVISPDGFMIF"
     gene            complement(4766..5650)
                     /locus_tag="VW006_001658"
     CDS             complement(4766..5650)
                     /locus_tag="VW006_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9380475.1"
                     /translation="MKKAMVIITPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEAIHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKLKED"
     gene            complement(5762..6616)
                     /locus_tag="VW006_001659"
     CDS             complement(5762..6616)
                     /locus_tag="VW006_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9380476.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(6674..7432)
                     /locus_tag="VW006_001660"
     CDS             complement(6674..7432)
                     /locus_tag="VW006_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9380477.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            complement(7494..7682)
                     /locus_tag="VW006_001661"
     CDS             complement(7494..7682)
                     /locus_tag="VW006_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9380478.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(8004..8570)
                     /locus_tag="VW006_001662"
     CDS             complement(8004..8570)
                     /locus_tag="VW006_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380479.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(8838..10517)
                     /locus_tag="VW006_001663"
                     /pseudo
     CDS             complement(8838..10517)
                     /locus_tag="VW006_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10609..10770)
                     /locus_tag="VW006_001664"
     CDS             complement(10609..10770)
                     /locus_tag="VW006_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380480.1"
                     /translation="MNNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(11454..11768)
                     /locus_tag="VW006_001665"
     CDS             complement(11454..11768)
                     /locus_tag="VW006_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380481.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(11734..>11866)
                     /locus_tag="VW006_001666"
     CDS             complement(11734..>11866)
                     /locus_tag="VW006_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9380482.1"
                     /translation="QALKNTEYKDQISSTVLPDIKIKLPIDSRGTPDWNYMERYRDR"
BASE COUNT         3528 a         2430 c         1624 g         4284 t
ORIGIN      
        1 gaggatgtat atcataaatc ggataatttt tcattatgat tgagactgcc atttgtaact
       61 ttttaatacc tttggtagta ttatgatcag ttactacaac tatttctatt ccatttttta
      121 tgattgcctc tgctaacata agaaaactaa tatattcttt tgaactagaa aaaacaacct
      181 tatcaaaatt actaaaaaaa attgtcttat caacttttat tttcttattt tcaaaaaata
      241 tatcatatag ttcatcttca gtaatttttc tatatttcgt tgcagtccaa ttagaaaata
      301 gcttgtaatc ataagaaaca ggtgtatgca aatgaattaa agtcttgtga aagctaccga
      361 atttctcttt ggaatttgtt agttcttgat aaacagactt tatcttgtta ttaaaccctc
      421 tcatttctat tctcctaaaa atatcaattg tttatttttc aagtaattca ttaagtctct
      481 tttcccccac ctgcaacatc tccttctcta cctgactcca ctccccaaag aataactctt
      541 gaagaacatc tgctgctgaa gttgtatttt cttttgaatc atatacacaa cttctatctc
      601 gttggtaaat ttgaattctt tcaaagatag ctagttcttc caattttcgt gtgttatcaa
      661 ctagatgatt tacaatgaaa tcatgatgtt cttttggagt tgcgcgtgct tgatttggat
      721 tgataatgta cagttcttca taacggataa gagtacttag atacgacaat tcaggctttg
      781 tcgcaattaa ggccaattgt acttcatatc ccttattttt caagagttgt gccgttttct
      841 ttggaacatc aattgttcgt aaagttccct cgatcaaaag attgtatccc aaactactca
      901 attttgttac taaagactct accatttttc ctgcaaaatc tttggtatat tctacactgt
      961 ctttgccata ttcttgctgc agttctaaat agtgtggatg ctgagaacga aaactatcac
     1021 catctatgat aacaatattt ccttgaaatt ctttctgttt aatacgatga attgtagtct
     1081 taccggcacc actttgacct ccaagcaaaa tcgctatagg ttgcttactg gacttttttc
     1141 ctcttgtcag tgaacgaaga ttccttgcta aagcatgttt gaattcacta tcagtataat
     1201 cttggatttc cattaagcta ccatccgctt ttcagatatc tctaacatac gttcaattcc
     1261 atccaaatag ccactatatc tctctatttc atcaaaagtt tctactaaat agatattcgt
     1321 atgaatcaaa tcagaaagat catcactcat taaaatccaa ggattagatt catcatcaat
     1381 gctaattccc tgactatctt gatagcgata gagtcgagat aatagattag cacctctttc
     1441 tttcacaatt tcaattttta aagtcaattc ataatcttca acaggattga gcattttatc
     1501 ttctcctacg atatcgacat aagatacatt aaacttctga caaatgatgt ctattaattc
     1561 ggtagagact gaactcgttc cattttcata acgactcaga ctatttcgtg aaatacctac
     1621 aatccgtgca aattcgtgtt gtgttaagtc atgtgtttta cgtaaggatt ttatgttctt
     1681 tccaatcatg gcaaactcct ttatttttat aattcaatta tagcataaaa aagcataatg
     1741 caccaaaaat ggtgcattat ttcttgtttt tctatcctaa actttacttc tataaaagtc
     1801 acctaaagag ccataaatat ttattcttgt attaattttt tcgatgcgca aacgatatct
     1861 acaaatacat ctataacgat tcacctttta ggatttttat tgtcaaaatt gcctttatct
     1921 acaaagacaa aatgcttata aaatagtttg aaatcaagga ttatcgcctt tttcgttgta
     1981 gcaacactcc acatgtgatt attaggctat tagcaaagcg tatccaatac ctcaataaag
     2041 tgttgttttt actgatattt taactatatt gtatagtcta ctatttaaat ctcaattagg
     2101 atattttata gaatacccaa aattcaacaa taactaaaag taagattcca ttgtatttcc
     2161 ttgttggtct ttcatatcac gaattttatt tttcagaata ttaaagttac aaatttcatc
     2221 tctaatatcg ttatccgctc tgtagccatt aatcatctca gtcagagttt tccctttact
     2281 accattcttt atagtagtat ttactctttt tatactgctt tcaaaaggct catatgtttt
     2341 atacttaatt acttcgtctt taaaattaaa taaaggtgta tccggcttag cattccataa
     2401 aataatcaac cacattatag cattataaag ctgctttgca gtttcattcc acttttcagg
     2461 ttataaattt ccactccctg tattgtcata ttcttgtcta ttatttacat gagaatcacc
     2521 tacaagcgga ttaaaaagag gtagcccttt gtgattaatt agttctaaaa aatcccttgc
     2581 cgcacccatg caacactgaa taacttcctt cttgccatca attttactct cagcgataaa
     2641 tacataatat tcatcttcaa tagttgctac tgcatcacta tgtttttgtt gatttgctaa
     2701 cagcttaata tgtactttag gctgaacttc aaaatctcta acaattcttt gtcttgtttc
     2761 atgtcctcta caattcattt aagttctcct ttacaagttt agtaactagc cttattatac
     2821 tatataaggt atgaaatgta ccataatctt catcaactag tttttagaaa atcataaatc
     2881 catctggaga gatgacaagc tgattatcca attcttggca attgcttgct agtgctactg
     2941 cttctttctc taaaagataa tcttctgttg attggttgaa tattgctttg aggatccgtc
     3001 cttcgtattt ccattccaga gctactagat cagagttgat ttcttgaagg ctatacttgc
     3061 catgcgaaat gattactgac gcgtatttcc gaatcttaat cagcctcttc ataaagttca
     3121 gcatatcatt gtcacttgat acacgttccc aaggcataca acgacgacaa tctggatctg
     3181 gtcctccagt caaggctagc tcggttccgt aataaatgca cggtgttcct ttttgtaaaa
     3241 agagaaaggc taaggctgat ttaaccagtt gaacatcttc attggccgtc cacaggattc
     3301 gctctgtatc atgtgaatcc aagagattaa acatgacctc tgaaatctgc tgcttgtaat
     3361 acatagactc tccattgatt tcatcgatga actggtctgt cttcttaatt cctcgtaaga
     3421 aatagtcctt gatactatca gataaaggat aattcatgac ggcatggaac tcatctccat
     3481 ttagccaagg ctgagatgta tgccagactt ctcctaggat ataaagatca ggatttttag
     3541 ctaaaactgc cttacgaaaa tccttccaga actgatggtc aatctcatta gccacatcca
     3601 aacgccaagc atcgatatta aactcttcaa tccaataagt cgcaaccttt aaaagataat
     3661 tcttgacctc tggattggct gtatttagct taggcatata gtcctcgaaa ccaaaaacat
     3721 gatagggtaa gtctctctta ttaactagct tttcagttgt cactgggaat tgttgaatat
     3781 ggaaccaatc cttataagca gactgttcac catttttgac gacatttttc cattgaagag
     3841 attgcgaacc aatatgatta aataccgcat ccagcatgac tttcatgcca cgatgatgcg
     3901 cttgatccac cagttcccga aatgtctcct tgtctccaaa atgacggtca atttcaaagt
     3961 aatctgtcgt attgtacttg tgattgcttg cagattcaaa gatgggacaa agatatagtc
     4021 cagtaatacc caagtcttgc aagtaatcca tatgatcaat aatcccctgt aaatcaccac
     4081 caaagaaatc atcgctctta ggtgtgacag atgaatccca gtctaaagtc ccttctgggt
     4141 ttaatagagc attgccattg gcaaatcttt caggaaatat ctgataccat accgtatttg
     4201 aaacccagtc aggaactttg caggcatcaa tctcatgaag ataaggcaac ttaaatccat
     4261 tcccaattgc atgaagattt tctagagaat tttccacaca ccctttatcg ccatacaaaa
     4321 tattttgacc ttctgtatct ctgagctcaa agagatactg gatacgtgca aagtcaactg
     4381 acacttcaac ctgccaatgg tcaaataagg taccagaagt tatcttgacc atttcttttg
     4441 tatcctgata aaactcctcc ataaagataa aagggtcccc atagtgcaag ttgatgcttt
     4501 caatgtcccc tttcttagtt cgaattcgaa tatggagttt cttatcctta taaagatagg
     4561 catactccga ctcaggccta tggtaaatag cactcaattc catcaatttg tctcctaaag
     4621 tattcattca aattttacgc aaacgttttc aaactcaata aatccagtat actatttttg
     4681 taaactattt gcaaggaatg tccgttgtta aaggaactat cttttaaagt atacaaaaag
     4741 ccttaaaaaa ggctttctct atatactaat cctctttcag ttttgaaaat acttctactc
     4801 gctgagctag gactttgatt tctacaggta ggttatctga tttatctccg tcaacatctg
     4861 actccaattc actatctgaa gagattttaa tattacgcgc tttgatatac tcgatattat
     4921 catttgcgac aacatcccct tttaataaat caggaatgac ggataatttg gagaatatag
     4981 aggcatcttt cagaatcaaa atgttggcat agccgtcctt gttttcttca aagattttct
     5041 tatcagcgaa gtaatttgtc aaaaggacca aaacatggat agcttcacca acataatttc
     5101 cattttctgt ctcaacctta atgttaaaga cctgatctgt catgacagac ttcatggtat
     5161 ttacagcata ggttaaaata ccgaattttg ttttgtcctc tatttcaaca ttgtgaatcg
     5221 cctcaggcag agaaccgata ctaaagatat aaccaaaata gttgtcattt gctttaccga
     5281 tatcaatctt attggttaaa tcaaaatcca gttcgtcaat tgcgccatcg atgtcttgat
     5341 tgatttccaa aagttttgta atgaggttac ccgtaccgcc tgggataatc cctaacttag
     5401 gaatgtagtc tctctcatca atacctgaaa tgacttcatt gacagttcca tctccaccaa
     5461 acacaaccac tgcatcatac tgctcacgag aagcttcttc agcaaaatgt gttgcatcca
     5521 gcgctttttc ggtaattttg gtttcaacat attcaaagta ttcttttgct ttattctcca
     5581 gcttttcttt gtaatccaaa gccttctcgc caccagaagt aggggtgata attaccattg
     5641 cttttttcat tgattttatc cttaatttta aacagaaatg tttacatttc gtcgtatgca
     5701 agtaaatgta atcctattat acaatgaaaa tacagaaaag agaaatctga cgtactggag
     5761 attaatacgc ttttattcta ttttcccatc gcctaactac atcctttaag ggttcatcca
     5821 agtaagaata ggccttatcc ttgatccaat caggaatacc gtaagctgcc tctgctatgc
     5881 tacaagtgat tgctgcgaga gtatcactgt cgccaccaag tgagatggca tttcttatcg
     5941 catcttcgaa gtctctactt tcaagaaagg cgataatggc ttgagggaca gtttcctgac
     6001 atgtttcgtt aaaacgatag ttaggacgga tttcatctaa agtttgagat agattgtaat
     6061 cgtattcttt ttctatataa tccttaattc gtcttttaca ctctgtagga ttgtcgttaa
     6121 ttgattgctc gtactctcta caataaccac caaagtaaaa acgacataga aagatagcat
     6181 cagctgtagc catagcgcct ttgacacctt ctggatgatt atgagttacc tctgcagaaa
     6241 gactcgtaag tcctctagat gatggccata taccagtttt cgcataaaaa ccacagtcca
     6301 tgatccaagc acatggagaa atacgcatag ctgatccatt cccaaagcta ttataaggct
     6361 cacggttatc gctgtttagc catgcattaa accgagcacc gtaatcagca ttcggataca
     6421 ttctgccata tttcttcatc gcgtcaatga agtcatcttt ttgtccacca ttcataattg
     6481 cttctgcaac agcacaggtc ataaccgtgt catctgtaaa aaagcagtcc ttccgaaata
     6541 aaggaaagtc ctttgttttg atattgttcc attcgtaaac agaaccgaca atatctccaa
     6601 taattgctcc aagcatcaga ttcctccttg ttcattttga tgctttttat attggttatc
     6661 taccatattt attttagaaa ataacatcct gttggatttt aaaaatttca tttttttcaa
     6721 aatagggttt taccatttct ttccacctag ctctatgaaa attgattgat tttaaaggag
     6781 ataggccata atttcccaat gcataaccat catttacttc aacaacaagt gttctgccat
     6841 cgcgagtaac accgatatct agtccataag ctattggcgc atctttccaa catgatatcg
     6901 cttcatcaat tacacttgca tcaaattgtg catgataatc acctgtatag ggtcgaacat
     6961 ctaatacgcg accatctaac acaaaacaac gccattcagc tatgaattct acaacctcac
     7021 taatccatat aggatagtcg aaaggtagac caatacctat taaatcatgg gttccattaa
     7081 caactcttcc agtaaagact tttgaaccag ctttaggctt aataaatttt ccccaattat
     7141 caggtatatt cacaatctct cctaaaatac cagcataaat ctttcgacca taaaactctt
     7201 taagctcaat aggatagtca tgaaccggaa cgtttaagcc catcattttt agtaatgctc
     7261 tagtctccat tatataatct acaactatat cttcacttgt taactctttt atttcagaaa
     7321 aagattgata taaaataact tcttctcctt gtaagtaggc acctacttga gcattgtatt
     7381 tattaattga aacctcactt ggtaatttac tttgtctaat ataaacaacc atttcatcac
     7441 tcctatatca ctagtgttac accaatttgt aaaaaataat agcaattttg ctcttatttt
     7501 tttgagtaaa tagcccccat aatatcatcg aaataatcaa cggtatttag gagtaattca
     7561 ataacctggg actttgttag tcgcattccc cttctatctc tagcatcttc tactaaattt
     7621 tcaagtttct ctagattttt atcatccaag ctaatcatta ttctattttt atcggttgcc
     7681 attttcatca cctcaagtta attctatcac aggtgtaaca ctagtgtcaa ctggctttta
     7741 taatacatta gtttaaaagt ggagaggatt tttaacacag taactttaaa tctttggtat
     7801 taaaaaattt tcacaatatt tatagaaata aaatctgtct caaatcagtt atcaaatcta
     7861 gtataaatta tgagcggcta ctctaatact ttccctctaa acaagaaaaa gacttacact
     7921 caagggtttt cttccccccc ttcgttataa cgttttgact cttttactag caaaggtata
     7981 tactcacaag gaactttggt tgactattga atctctccaa cttcttcttt aacatatcct
     8041 tctacatctt caatctctac aaacattggg tctaagtgac acaagaaatg ccaaacttcg
     8101 atcccttttt ttctgtaaag aatcgcttca ccgtcttcac ttccgaaaaa gcttctgtcg
     8161 atttcatatc cgcggctttc taagaagtct tttgctttac gatagttcgt ttctcttgtt
     8221 tcgacatagg ctttaacttc atggttgtta acgacatatg catcaatttt tgaatatcct
     8281 tcgatcactc tatcattttt gagggataaa tttgaaatct ctttccaaat aatgtttaca
     8341 ttttcctcag gatcgaacat aaatttagat aaaggaacaa tatttccgtt aaaaataatt
     8401 tccatataat ccggtatgtt tttaggatta aaatactcca cttcaaaacc atcttctgtt
     8461 tccagagtgt atcccgggat ttgagctaca aaggctttcc catcttctat ggaatcaaat
     8521 gctactaaat ctttagaata atcattttgg tacaattcca atataaccat cgataatctc
     8581 tccattttca ttatcaggct aatgtaaata agcacgtcac ctgaccaatt caggctctct
     8641 gtatcatctc atcatatttc ctacttactt tacgagtctt atacccagaa cacaccttat
     8701 cgaccttcgg tctcacctcg tcgcattggc tgaacatcta cttttacttt gctgatgctt
     8761 caactcgtac aagcagtgat accgcctcag cgtgatgcgt cagtgggact caaaaggttc
     8821 ggggaacctt ttgaggatta actacgtttc tctaataaac ttacacattc aacttgttca
     8881 tcattgtcca aacctatgtt gagattttct tctataattg gtagcttaaa agtaatggat
     8941 tttagccatt gtccgttaga ttgtttttct tcataaactt gaatttcaga aatcaaagct
     9001 gaaattaact gcctacgctc tacatcattc atgactttat agagcttatc aaaatagatc
     9061 agaaccttat atatgttatc tcctgtaagc ttttcagctt caatagtctg tttctttgct
     9121 ttcgcatcaa ttagtgatga ttctaattca tctagtttgt catacatacg atatagtcta
     9181 tcatctaaat cctgtttcct tctcttataa tgcttatctt caacatctaa attatctatt
     9241 tcctcaatta gcttaaactt tgtagaatga ctctttctca attccttttg gtaattatct
     9301 atttctcttt ctatttcaga ggtatccacc ttcatgttga ttttttcttg catcatagaa
     9361 gcaaatttcg gattacttac tatcttgaca atcacctctg caacagcatc atctaacaat
     9421 tcttctctaa tttgcttact gaatgtacac ttatgacctc ttatcatctg cctatgttta
     9481 caaccatagt aataaaaatc tttatacttt gtgccatctt tctttttctt gatacacttg
     9541 ttcccaaaca ttcccactcc acatatcggg cattttacaa ttccagaaag caagtgtgtg
     9601 cgtgtatctt ttcctttatt cacatgctca tatttctttg cttgagattt tagcttaacc
     9661 tgagcagctt accaaacttc atcggaaact atagcttcat gtatcccttc agatattaga
     9721 tattcatctt gttcaacctg cttatattca tttcttgtac catgaacttt ttctaaagtt
     9781 cttcttccaa atgctatttt cccattatat acaggattct ttaatatctt tcttataaga
     9841 cctgcatcaa acaaaggatt cttaccattc tgtcttggga tttttctaat tccatgattc
     9901 tctaagtatt tagatatccc attggctcct atcgtagtat ttacatactg gtcgaaaatc
     9961 gttcttattg caactgcctc ttcctcattt ataaacagct tgccgtcttc aagtttatat
    10021 ccatacggag caaagccacc attccatttt ccttcccttg ctttttgaat gcaaccttcc
    10081 attgtttgaa tacggatgtt ttctctttct atttcagcca cagctgataa aacagaaatc
    10141 attagtttcc cagcatcttt agatgaatca atgccatctt caacgcagat aagattaact
    10201 ccataatcct gcattatctg aagtgtagaa agaacatcag cggcatttct tgcaaatctt
    10261 gataacttaa acacaagaac aaaagatact ccatcttttc cagattttat atcttccatc
    10321 attcgattga actgtattct accttcaata gacttgccag acttcccggc atcttcatac
    10381 tctccaacaa tttcataatc gttgtaaata gcaaaagctt tcattcgtga tttttgtgcc
    10441 cctaacgaat acccctctat ctgtatcgac gtagatactc gtgtatagag gtatactttt
    10501 attttttctt ttgacatagt attaacctca atataatttt tctatatcat atataatttt
    10561 tttaatttaa gtttggacta tcatttcaag tatattataa cacttttatt agtccgtctc
    10621 aatttgtgtt tttgccatgt caaaactatt tttcatctct tgattttttg ctggcgttgg
    10681 atcgggtaga ttatctaaat ctaaagcacc agcatatttt gcaatcagat ttgctattaa
    10741 atcagccaat ccattccagt cattgttcaa tatatacctc ctctaaagtt ttatatctaa
    10801 taattatttg tttaattaag ttttttgaca ttgacaagtg ctttggatta gcaacatagg
    10861 aatctcacct ccgcctctat tccggatgag ccggcttcaa ccttagaagt atcattatcc
    10921 tcattttctt catagcggat agggtatccc tccctatatt caaactctta cttatcgctc
    10981 actttctttt tgtttagcag aacttttttt gccgaattat tcagccgaaa gatcttgacg
    11041 gataggttat tacgctccaa aaataattaa cgtcttgtct tggtctattc aattgttaag
    11101 gttcaaaatt tatcgaaagt tattaatctt tttaaaattt gaccatcaga aaatatttat
    11161 cttgatgtaa caaaattcta taaattaccc tcttatactt aacagtgaaa agaagtcttt
    11221 cttggtaacc aattttgaaa tagaatttgc ttatataaaa aggtccaatt cccactgcat
    11281 aagtagcagt gaaaattaga ccctcttggt aactgtcatc taaaagtctt ctagcatttc
    11341 tttaattttc taatcgcctt taaagtgtca tatttatatt tttttatctc tggcatactt
    11401 tgataccttt ttagacttaa agtctttaat agtgcctttc cacctctttt tatctataaa
    11461 gattctccta catcataatt cattttttta tttaaacctt tctgtcttag tttgtcttta
    11521 tcttcttcat accattttaa gattgtcaca tagtggtttt gataggtctt accactgctt
    11581 tccatgtatc tggatagttt atttatcatt atatctgtgt gtgagtttaa tttttctttt
    11641 agatttttat attcttcttt gcttaacctt acatttttga attctccata aaaaatgggg
    11701 gtggactttt tatctatctc tccctctctc tctttatcta tctctatatc tttccatgta
    11761 attccaatct ggagtacctc tactgtctat cggtaattta attttgatat ctggcaatac
    11821 tgtgctagat atttgatctt tatattcagt attttttaaa gcttgc
//
LOCUS       DAVKFH010000057        11652 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKFH010000057 DAVKFH010000000
VERSION     DAVKFH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.057"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(126..842)
                     /locus_tag="VW006_001667"
     CDS             complement(126..842)
                     /locus_tag="VW006_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9380483.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1190..2977
                     /locus_tag="VW006_001668"
     CDS             1190..2977
                     /locus_tag="VW006_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9380484.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2974..3450
                     /locus_tag="VW006_001669"
     CDS             2974..3450
                     /locus_tag="VW006_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9380485.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVSLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3478..4383
                     /locus_tag="VW006_001670"
     CDS             3478..4383
                     /locus_tag="VW006_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9380486.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4370..5185
                     /locus_tag="VW006_001671"
     CDS             4370..5185
                     /locus_tag="VW006_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9380487.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALTLSALGHFALGM"
     gene            5192..5596
                     /locus_tag="VW006_001672"
     CDS             5192..5596
                     /locus_tag="VW006_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9380488.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIGGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            5894..7060
                     /locus_tag="VW006_001673"
     CDS             5894..7060
                     /locus_tag="VW006_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9380489.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKKLVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALDCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            7176..8213
                     /locus_tag="VW006_001674"
     CDS             7176..8213
                     /locus_tag="VW006_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9380490.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            8420..9274
                     /locus_tag="VW006_001675"
     CDS             8420..9274
                     /locus_tag="VW006_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9380491.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            9424..9681
                     /locus_tag="VW006_001676"
     CDS             9424..9681
                     /locus_tag="VW006_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9380492.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADLVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(9723..10040)
                     /locus_tag="VW006_001677"
     CDS             complement(9723..10040)
                     /locus_tag="VW006_001677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380493.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDRTDEVSNHIYGKVKLTWFEEI
                     FEEYKSPHNKTHTIKVFMYCPPKVRQKKSNFWGQYISIPDTMRFFNFKDFLQLLLTHI
                     KLF"
     gene            complement(10112..10390)
                     /locus_tag="VW006_001678"
     CDS             complement(10112..10390)
                     /locus_tag="VW006_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9380494.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNA"
     gene            10612..11376
                     /locus_tag="VW006_001679"
     CDS             10612..11376
                     /locus_tag="VW006_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9380495.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT         3267 a         2166 c         2647 g         3572 t
ORIGIN      
        1 ttggttctat ttctataaaa tattccacaa attatagaat cttccaaaaa taggtaagcg
       61 ctaccttttt ggtgtggtat aataagcata gaaaaagccc gagcgattag gctcaggctt
      121 tcttcttagt gatcacggtc acatgagatg aatttaatct tgtagtaatc agatcgtttg
      181 taagtttcac tgtattctaa aacttggcca gttgattcga gtttggtgat tttagtttgt
      241 aggacagtag ggaattgttc atcgactccg aggactgaag ccgcatgttc tggagttgga
      301 aagactattt cgttgatttc ttcaaagtgt tcatcattca tgtgaatgtg gtagtctaac
      361 ttgaaacgat tatagataga actatagtat tcaaggtttg gataatttgc gttgatatat
      421 tgttctggga tgtaggatgt atggtagata taaacgacac cgtttgattc gcggatacgt
      481 tcaatcttgt agtagaattg atcgccgcgt agacccaatt tttccaagta aacaagcttg
      541 tttccgcgtt caattgaaag aacagttacc ttatcatctt tagcattgaa gagttcaata
      601 tctgaaaact ctacaagctt gtgtttgcgt gcacgtgaaa cgaaggttcc ttttccttgt
      661 tggcggacaa tatagccatc tttggcaagg tcgtttaagg cgcgaacaac tgtgatagag
      721 ctgacatcgt acattgaaat gagttctgct tcagtgtaaa atttatctcc actgctaaac
      781 tgcccagaga tgattttatt ttttaattcg tcttttatgt attgatattt gggaattgcc
      841 atatttttac ctctattttc cttctccata gaaaatttta ccataaaagc gaaaaaaata
      901 gtagtctttt gaataaaaaa ttagaataat agtttgaaaa attaagcgat atagggattg
      961 ctcatatttt atcaatgcat tctatacaaa attattttag gtcctatatt ccgaatttaa
     1021 agagcgtttg aaatgggtct aaaaatgttt gaaactaatt gaatgtgtgt ataatagtaa
     1081 aaatgcagta aatatcccga caatttctat tgacaattca aacagattgg tttataatta
     1141 atataacaac aaatgaaagc gcaaactttc gcggtcggaa ggtagtttta tgacacgatt
     1201 tgagatacga gatgatttct atctcgatgg aaaatcattt aagattttat ctggtgccat
     1261 tcattatttt aggattcctc cagaggattg gtatcattcg ctctataact tgaaggctct
     1321 tggttttaat acggtagaga cttatgttgc ttggaattta cacgagcctc gtgaaggtga
     1381 gtttcatttt gaaggtgatc tggatttaga gaaatttctc caaatagcgc aggatttggg
     1441 tctctacgca attgtgcgtc cgtctccatt tatctgtgcg gaatgggaat tcggtggctt
     1501 accagcttgg ctcttgacca agaacatgcg aattcgctca tccgacccag catatatcga
     1561 ggcagttggt cgctactatg atcagttatt gccaagactg gtgcctcgtt tgttgaacaa
     1621 tggtggcaat attctcatga tgcaggttga aaatgagtat ggttcttacg gagaagataa
     1681 ggcttacctg agagcgattc gacagctaat ggaagagtgt ggcgtaacct gtcccctctt
     1741 tacatcagat ggtccatggc gagctactct gaaagctgga accttaattg aagaggacct
     1801 ctttgtaaca ggaaactttg gttctaaggc accttacaac ttttcgcaga tgcaggaatt
     1861 ctttgatgaa catggtaaga aatggccact catgtgtatg gagttctggg atggttggtt
     1921 caatcgctgg aaagaaccga ttatcacacg ggatcctaag gaattggcag atgcagttcg
     1981 agaggttttg gaacaaggct ctatcaatct ttacatgttc cacggtggta caaactttgg
     2041 tttcatgaat ggttgctcag ctcgaggaac tttggacctg ccacaagtta catcttatga
     2101 ttacgatgcc cttctggatg aagaaggaaa tccaactgct aaatatcttg cagtcaagaa
     2161 gatgatggca acacattttt cagagtatcc gcagttggaa ccactctaca aagagagtat
     2221 ggagttggat gctattccac tagttgaaaa agtttctttg tttgaaacct tagatagctt
     2281 gtcaagtcct gtagaaagtc tctatcctca aaagatggag gagctgggac aaagttatgg
     2341 ctacctactt tatcgaacag aaacaaactg ggatgcagaa gaagaaagac ttcgtatcat
     2401 tgatggtcga gatagggccc agctgtatgt cgatggtcag tgggttaaaa ctcaatatca
     2461 gacagagatt ggggaagata ttttttatca aggtaaaaag aaagggctat ctaggttaga
     2521 tatcttgata gaaaatatgg ggcgtgtcaa ctatgggcat aagttcttag cggatacgca
     2581 acgtaaggga attcggacag gggtctgtaa ggatctgcat ttcttactaa actggaaaca
     2641 ctatccactc ccactagaca atcctgagaa aattgatttt tcaaaaggat ggactcaagg
     2701 acaaccagcc ttttacgctt atgactttac agtcgaagag ccaaaagata cttacctaga
     2761 cttgtctgag tttggtaagg gagttgcctt tgtcaatggg cagaatctag gacgtttttg
     2821 gaacgttggc ccaactctct cactttatat ccctcatagc tatctcaagg aaggtgccaa
     2881 ccgtatcatt atctttgaaa cagaaggtca atataaagaa gagattcatt taactcgtaa
     2941 acctacacta aaacatataa agggggaaaa cttatgacaa ttgtaggatg ccgtattgat
     3001 ggacgtttga tccacggaca agtagccaat ctttgggctg gaaaactaaa tgtttcacgc
     3061 attatggttg tagacgacga agttgtcaac aacgatattg aaaagagtgg tttgaaactt
     3121 gcgacaccac caggtgtgaa attgagtatt ttgccagttg agaaagctgc agccaatatt
     3181 cttgctggta aatacgatag ccaacgtctc tttatcgtgg ctcgtaaacc agaccgcttc
     3241 cttggtttgg tagaagcagg tgtatcactt gaaaccctta atgttgggaa tatgtctcaa
     3301 acaccagaaa ctcgttctat tacacgttct atcaacgtag tagacaagga tgtggaagac
     3361 ttccacaaac tggcagaaaa aggtgttaaa cttactgctc agatggttcc aaatgatcca
     3421 atttcagact ttttgagctt attaaaatag gaaaaaaatt tttaggaggt cattgttatg
     3481 atacaatggt ggcaaatttt acttctcact ttgtactcag cttatcaaat ctgtgatgag
     3541 ttgacgatcg tttcatctgc aggttcccct gtatttgctg gtttcattac tggtttaatc
     3601 atgggagatg tgactactgg tttacttatc ggtggtaact tgcaactgtt cgttcttggg
     3661 gttggtacct tcggtggtgc ttctcgtatc gacgcaactt ctggtgcggt tcttgcgaca
     3721 gccttctctg tttcacaagg aattgatgca ccgcttgcca ttactacaat cgctgtacca
     3781 gtagcagctc tcttgactta cttcgacgtt cttggtcgta tgactactac cttcttcgct
     3841 caccgtgtgg atgctgcaat cgaacgcttt gactataaag gtattgaacg caactacttg
     3901 cttggtgcga ttccgtgggc tctatctcgt gcccttccag tcttctttgc ccttgctttt
     3961 ggtggtgcct ttgtacaatc agtagtagac ttcgttgaag cctacaaatg ggttgcagat
     4021 ggcttgacac ttgcaggacg tatgcttcca ggtcttggat ttgcaatctt gcttcgttac
     4081 cttccagtta aacgtaacct tcactacctt gctatgggat ttggtttgac agctatgttg
     4141 actgttcttt actcatatgt aacaggtctt ggtggcgctg ttgctggtat cgtaggtact
     4201 cttcctgctg aagttgctga aaaaattggt ttcgtgaaca acttcaaagg tttgtctatg
     4261 attggtattt ctatcgtagg tattttcctt gcagtgcttc acttcaaaaa tagccaaaaa
     4321 gtagctgtag cagcaccttc tacaccatca gaaagtgggg aaatcgaaga tgacgaattc
     4381 taattacaaa cttacaaaag aagattttaa tcaaatcaac aaacgtagct tgtttacttt
     4441 ccaattaggt tggaactacg aacgtatgca agcttctggt tacctttaca tgatcttgcc
     4501 tcagttgcgt aaaatgtatg gtgatggaac tcctgaattg aaagaaatga tgaaagttca
     4561 tactcaattc ttcaatactt caccattctt ccataccatt atcgctggtt ttgaccttgc
     4621 catggaagaa aaagatggtg taggttcaaa agacgctgtt aacggtatca agacaggttt
     4681 gatgggacca ttcgctcctc ttggggatac aatctttggt tcacttgtac ctgctatcat
     4741 ggggtcagtc gcagcaacta tggctatcgc tggccaacct tgggggatct tcctttggat
     4801 tgcagttgca gtagcgtatg acatcttccg ttggaaacag ttggaatttg cttacaaaga
     4861 aggggttaac cttatcaaca acatgcaaag taccttgaca gctttgattg acgctgcatc
     4921 tgtacttggt gtcttcatga tgggtgctct tgtagcaaca gtgattaact ttgaaatttc
     4981 ttacaagttg ccaatcggtg aaaagatgat tgatttccaa gacatcttga accaaatctt
     5041 cccacgtttg cttccagcaa tctttactgc ctttatcttc tggttgcttg gtaagaaagg
     5101 tatgaactct actaaagcta tcggtattat tatcgtactt gctttgactc tttctgccct
     5161 tggtcacttt gcacttggaa tgtaattcct tatgactaaa tcattaattt tggtgagcca
     5221 tggtcgcttc tgtgaggagc ttagaggtag cacagaaatg attatgggcc cacaagacaa
     5281 catttacaca gtagctcttc ttccagaaga tggcccagaa gaatttactg ctaaatttga
     5341 agctgttatt ggaggattgg atgatttcct agtctttgcg gatcttctcg gtgggacacc
     5401 ttgtaatgtg gtgagtcgct tgatcatgga aggtcgtgat attgaccttt acgcagggat
     5461 gaatcttcca atggtgattg aatttatcaa tgcgagcctt acaggcgcag atgcggacta
     5521 caagagccgt gctgcagaaa gcattgtgaa agttaatgac ctgttagcgg gcttcgatga
     5581 tgacgaagat gaataatact cttcgaaaat ctcttcaaac tacgtcaacg tcgccttgcc
     5641 gtaggtatat gttactgact tcgtcagtct tatccggcaa cctcaaaacg gtgttttgag
     5701 ctgacttcgt cagttctatc tgcaacctca aagtagtgct ttgagcagcc tgcggctagt
     5761 ttcctacaga ttttagttgg aactcgattc aattcatgtg acaacgtgaa aatcgttaga
     5821 gcattttata tagaatatac atgggaatgg agcttactcc cattcccata tttaatagaa
     5881 aaagaggaac tcaatgctac attatacaaa agaagacttg ctcgaattgg gtgcagaaat
     5941 cactacgcgt gaaatctacc aacaacctga tgtatggaga gaagcttttg aattttatca
     6001 agcaaaacgt gaagaaattg cagccttcct acaagaaatc gctgataaac atgactatat
     6061 taaggttatc ttgacaggtg ctgggacttc tgcttatgtg ggagacacct tgctacctta
     6121 ctttaaggaa gtctatgacg aacgcaaatg gaatttcaat gctattgcga caacagatat
     6181 cgttgccaat ccagcaacct atttgaaaaa agatgtggca actgtccttg tgtcttttgc
     6241 tcgtagtggg aactcgcctg aaagtgtggc gactgttgat ttggccaaat ccttggtgga
     6301 tgagctttat caagtgacga ttacttgtgc agcagatggt aaattggctc ttcaagctca
     6361 cggtgatgac cgtaatctct tgctcttgca accagctgtc tctaatgatg ctggatttgc
     6421 catgacttct agctttacgt ctatgatgtt gacagctctc ttggtctttg atcctacaga
     6481 atttgctgtt aagtctgaac gttttgaagt tgtatctagt cttgcccgta aagttttaga
     6541 caaggcagaa gatgtcaaaa agctcgttga tttagacttt aaccgtgtca tctatctagg
     6601 cgctggtcct ttctttggac ttgctcatga agctcagctc aagattttgg aattaactgc
     6661 tggtcaagtt gcgaccatgt atgaaagccc agttggcttc cgtcacggtc caaaatctct
     6721 tatcaacgac aatacagttg ttttggtctt tggtacaacg acagactaca ctcgtaagta
     6781 cgacttggac ttggttcgtg aagttgctgg tgaccagatt gctcgtcgtg ttgtgctttt
     6841 gagtgatcaa gcttttggtc ttgaaaatgt caaagaagtg gcccttgatt gtggcggtgt
     6901 cttgaatgat atttaccgtg tcttccctta catcgtttat gcccaactct ttgccctatt
     6961 gacttcactc aaggtagaaa ataaaccaga tacaccgtct cctacaggta cagtaaaccg
     7021 tgtagtacaa ggtgtcataa ttcacgaata tcaaaagtaa gacagtgttt atgaattctt
     7081 gacaagagga tttgtaaatt atcagataaa ccatagattg tcagtacgct ttctatggtt
     7141 tgtttgcttg agagaaatag taaaaggaga acagaatgaa agcatacaca gagcgtgtat
     7201 ttggaaatgt tgagggcgag gatgtcttgg cctatcgatt tgagacagac ggtggctacc
     7261 aacttgaggt tatgacttat ggcgcgacca tcttgcgcta tgtcgcacct gacaaggctg
     7321 gaaattttgc caatgttatc ttgggatttg atgactttga tagttatgta ggcaatagtc
     7381 ccaagcatgg agcaagtgta ggtcctgtag cgggtcgtat tgcaggtgcg acctttgagc
     7441 tcaatggtaa gacctatgac cttgaggtta ataatgctag caactgtaat cacagtggtt
     7501 caactggttg ggattccagc ttgtttgaag ttgaagaagt aagcgatcat ggcttgactc
     7561 tctacacaga gcgtacagat gggacaggag ggttccctgg aaatctcaag atttggatca
     7621 gttatcactt ggaagaaact ggtgcctatg aaatcagcta caaggtaacg accgatcagg
     7681 atacgctggt caatccaacc aaccacagct atttcaactt gtctggtgat ttcacgcaga
     7741 cgattgaccg tcatgtcttc caactaaaca cagagggcat ttactcaatc gctcctgacg
     7801 gtgttcctgc caaaactcca gaagccaacc gtgatgtggt caaacacatc tacaatggtg
     7861 ccttgttgaa ggatatcttt gcagaagaag atgagcaaat ccagctggca tcaggtttgg
     7921 atcatccatt tgcccttcct gcaggccatg acaatgctgg attcctttat gaccaaaatt
     7981 caggtcgctt cctgcttttc aagacagaag ctccttgctt tgtggtctac acagcaaact
     8041 ttgtggatga aagtgtcatc ataggaggtc agccaatgct acagcacaat gggattgctc
     8101 ttgaagcgca agctttacca gatgccattc acagtgacct taaaggccaa gtcattctta
     8161 aagctggtca aaccttcacc agtaagacac gttatgaact tgttgtgaaa taaaaaagct
     8221 ggtcaaaaag tctttaaaat taaaataacg catattgtca ggtgatgaaa agtttgataa
     8281 tatgcgtttt attatgaaat gatttacttt atattatcct gaaatggtgt tatttttgag
     8341 ttatctggct tgactttctt gatggaaatt atataatagt tgcaacaata ataattacaa
     8401 cgaaaaaaga ggaccattta tgacctatga atacaagagt cacatttatt tggcagagac
     8461 agttttaaat gtaaaggatt tggccagtca aacagccttt tatcagcaag ttatcggttt
     8521 agaaatctta tctcaaactg agacagagtc cattctaggc cttggtggaa aagtcttggt
     8581 acagctgatt caagcacaag agagcggaga agtgagggaa cattatggtc tttaccattt
     8641 ggccattctc ttgcccacac gcaaggcttt ggctgatgtc ttgaaacacc tgacagattt
     8701 acagattcct cttgtcggcg gtgcagacca tggttacagt gaagctattt acttggaaga
     8761 tttggaggga aatggcattg aactctatcg agataagcca gtttccacat gggatattcg
     8821 agaagatgga cgcattatcg gagtgacgga ggctcttgcg gcgcaggaca tttatgagtt
     8881 gggggaaaga gtagagccct ttatcctggc agaaggtacg agaatggggc atattcatct
     8941 ttcagtcaag gatagtcgaa agtccagcca gttttatcaa aaggtgttag ggctagagga
     9001 taaattcagt gtgcctagcg ctagttggat tgcggctgga gattaccatc atcatttagc
     9061 agttaacgaa tggggaggaa aaggtctggc ttcgcgtaag caaggcttac caggtttagc
     9121 ctactatgtc atcgaagtcg cacataaaaa agaactgtta acgattgccc aacgagcaca
     9181 agaagttgac gcaccaatca aatggatgac atcgagccaa ttggaaatca cagactcaga
     9241 tggcatcgtg acccgtattc gtttagcaag gtaaaattag gaatttttaa taagttataa
     9301 gtattgctag tttgcttttt gaacggtctt tggtaacaga actgcgtgag ctggatgggt
     9361 gagaattttg acaaagaagc tgattagaga tataatgaag aaaagtcgag caaggaaaaa
     9421 gaaatgacaa attcaaagta tattacttgt ttgaaacgtt cagagggcca gttgtgtggg
     9481 attcaaaaga tgattgaagg agatcgtgac tgtgctgatc ttgtgacgca actgacagca
     9541 gtgagatcta gtgtagagcg cgtgattgag atgataatta ccgaaaatct tactgaatgt
     9601 atcaatcagc cactagatga ttctgaagct caaaaggaac gcttagaaaa ggctatccga
     9661 tacttgatta aacggaaata aatgaatgtt ttgagacgat atgtgcaaag gtctattaac
     9721 tattagaata gtttaatgtg agtcaaaaga agttgtaaaa aatctttaaa attaaaaaaa
     9781 cgcatagtat caggtattga aatgtactgc ccccaaaagt tagatttttt ctgtctaact
     9841 tttggggggc agtacataaa aaccttgata gtatgcgttt tattgtgggg agatttatac
     9901 tcttcgaaaa tctcttcaaa ccacgtcagc ttcaccttgc cgtagatatg gttactgact
     9961 tcgtcagttc tatccacaac cttaaaacag tgttttgagc agcctgcggc taacttccta
    10021 gtttgcactt taattttcat tgagtattac ttcatcttct catgaaattg agtttttgcc
    10081 cagctcctct tgattattct acttgaccgg gctaagcatt cataccacct tctacgttgg
    10141 taacggtgag gccttgggag ctgagaaatt ggcaggtaga tgcagagcgg actcctcctt
    10201 gacaaataac atagtattca tggtcaggtt tgagttcttt gtagccttgc tctaaggtac
    10261 ttaatggaag atttttggca cctggtgcat gtcctgcttg gaattcatgt gcttcacgga
    10321 catcgataag ttctagattt tcattttgat attttccata aaagtcagcc atgctgatac
    10381 tagtttccat attctactcc ttctgggtta gccattttgt atagtgaata agcgccgtca
    10441 aggttttgga cggtaaatcc tgcttgcttg aggataggct ctgcgatata gctgcgcaaa
    10501 ctactgtggc aactgacgat gtaggcttgt ttttctcctc agttcatcag cttcctcctt
    10561 gacactcggt cagattttga tacaatagta caaaattaga ggaggtgggc tatgattcag
    10621 aaacatgcga ttcccatttt agagtttgat gataatcctc aggcggttat catgcccaat
    10681 cacgaggggt tggatttgca gttgccaaag aagtgtgtct atgctttttt aggtgaggag
    10741 attgaccgct atgcgaggga agtaggggcg aactgtgttg gcgaattcgt ttctgctacc
    10801 aagacctatc cagtctatgt catcaactac aagggtgagg aggtctgtct ggctcaggct
    10861 cctgttggct ccgctccagc agcccagttt atggattggt tgattggcta tggtgtggag
    10921 cagattatct ctactgggac ctgtggtgtc ctagctgata tagaggaaaa tgcctttcta
    10981 gtccctgttc gcgctttgcg agatgaggga gccagttacc actatgtggc accttgtcgt
    11041 tatatggaaa tgcagccaga ggctattgct gctattgagg aagttttgga agacagaggg
    11101 attccttatg aagaagtcat gacctggacg acagacggtt tttaccgaga aacggctgaa
    11161 aaggtggctt atcgtaagga agaaggctgt gctgttgtgg agatggagtg ttctgctctt
    11221 gctgcagtag ctcaattgcg tggggttctc tggggtgaat tgttgttcac agcagattct
    11281 ctagcggact tggaccagta cgacagtcgt gactggggct cggaagcttt taataaggcg
    11341 ctagaactga gtttagcaag tgttcaccac ctttagttgt actggcaaag gatttgtttt
    11401 atcataaaat gtctagctca tacttttcaa aaatatgttt aaacgaggtc accttcctct
    11461 tgtcctaggc atgttgaggt tgggaaaaat ctttaaaatc agaaaaacgt atcatatcag
    11521 gtgatgaaaa ttttgatact atgcgtttta tgttggtaag atttagagtg agatgaaatg
    11581 atactcttcg aaaatctctt caaaccaggt cagcttcacc ttgccgtata gatgttactg
    11641 acttcgtcag tt
//
LOCUS       DAVKFH010000058        11643 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKFH010000058 DAVKFH010000000
VERSION     DAVKFH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.058"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            106..795
                     /locus_tag="VW006_001680"
     CDS             106..795
                     /locus_tag="VW006_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9380496.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPK
                     EIAQRFINVIK"
     gene            820..1737
                     /locus_tag="VW006_001681"
     CDS             820..1737
                     /locus_tag="VW006_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU9380497.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            1765..3306
                     /locus_tag="VW006_001682"
     CDS             1765..3306
                     /locus_tag="VW006_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU9380498.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAIAIVYSYTGGLKSVLWTDFIQGVVLISGVVVALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLAIATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGVTGL
                     ILAAIYAAAQSTVSTGLNSVATSWTLDIQDVISKNMSEDRRTKIAQFVSLAVGLFSIV
                     VSIIMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTENKVSAPKYTTIHDISEIKADSS
                     WEVRH"
     gene            3332..3790
                     /locus_tag="VW006_001683"
     CDS             3332..3790
                     /locus_tag="VW006_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9380499.1"
                     /translation="MIISEQSDFRRYASVNKHFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            3792..6014
                     /locus_tag="VW006_001684"
     CDS             3792..6014
                     /locus_tag="VW006_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9380500.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTSISEVQHKSGNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVTRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPAPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIREHGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTQVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQISIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6054..7163
                     /locus_tag="VW006_001685"
     CDS             6054..7163
                     /locus_tag="VW006_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9380501.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            7320..8210
                     /locus_tag="VW006_001686"
     CDS             7320..8210
                     /locus_tag="VW006_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9380502.1"
                     /translation="MKIIGIDIGGTTIKADLYDDFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYVLNYSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTSEIEKRF
                     GLSTFVENDVNCAALGELWEGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLSSGDKVAKETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNVVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            8235..8558
                     /locus_tag="VW006_001687"
     CDS             8235..8558
                     /locus_tag="VW006_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380503.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELISIESNLEMKQQNLIDEVNKTIKENDAKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            8548..10539
                     /locus_tag="VW006_001688"
     CDS             8548..10539
                     /locus_tag="VW006_001688"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9380504.1"
                     /translation="MAISQMKRISLLFTKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFSTIQISHTSNSHHGVIEGNDALNYLMNKQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERNIQSNNEEIERLIKW
                     EKLEIIPAHLEQFSFCRGKVGTIPRTVDNRLYNNFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGIMLALKIFHLPSVTKKFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ITTTSDVMTILVVSVVFGFITVFVGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDIRPLFVLGKWVSIFNAVGILIASIIQAKSLSGIGSGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            10555..11031
                     /locus_tag="VW006_001689"
     CDS             10555..11031
                     /locus_tag="VW006_001689"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU9380505.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11065..>11643
                     /locus_tag="VW006_001690"
     CDS             11065..>11643
                     /locus_tag="VW006_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380506.1"
                     /translation="MSNLENLRESVIEQAHEKGSMKLLDSKKKIDDEFEMQKSVIVKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
BASE COUNT         3922 a         1658 c         2323 g         3740 t
ORIGIN      
        1 ttcctgatag ggctgttcat atcttatttc aattcactat ataaataaaa ttgtagtaga
       61 tgttattgaa ttagtaaaaa aaataaaaaa acggaggttt tctgaatgga aactaaaaaa
      121 attaaaaatt taaaaggtca aataattgtt tcctgccaag cgttagaagg agagccactt
      181 tatactccaa atggaggagt gatgccgtta ttagcaaaag ccgcctttca agcaggcgct
      241 aaaggtattc gtgctaattc tgtccgcgat atttcagaaa taaaagaaga agttgactta
      301 ccaattatag gtatcataaa aagagattac gatggctttg aaccttttat ttcagcaact
      361 atgaaagaga ttgatgaatt agttagtgaa ggagtagaca ttttagcttt ggattgtact
      421 aatcgttcaa gaccaggtta tgataatatt actgacttta ttcatgatat taaagtcaag
      481 tatccaaacc aattattaat ggctgatatt tctacttttg aagaagggaa agtagctgct
      541 gaatcgggag tagattttgt gggtacgaca ttatctggtt atacaccata tagccctaaa
      601 aaagataatc ctgattttga gttagttgaa agattggtaa aagagcttga cgtaccagtt
      661 attgctgaag gaagaataag tactccggaa caagctagaa aaatgttaga tttaggtgct
      721 tacgcagtgg ttgttggagg agctatcact cgtccaaaag aaattgctca gcgatttatt
      781 aatgtcatca aataaaaaac aatataaagg agaaatctga tgaaagattt aactaaatac
      841 aaaggcgtta ttcctgcatt ttatgcttgc tatgatgaaa atggtgaaat tagccaagaa
      901 cgtgtaaaat ctctggtgca atatttcatt gacaaaggtg taaaaggtat ctatgtaaat
      961 ggttcttcag gtgaatgtat ttaccaaagt gtagaagatc gtaaacaaat tattgaagct
     1021 gttatggaag ttgctaaagg taaattaaca gttatcaacc atgttgcatg taataacacg
     1081 aaagatagta ttgaattggc aaaacattca gaaagtgttg gagtcgatgc tattgctgct
     1141 atcccaccta tttacttcaa attgccagaa tattcaattg cagcatattg gaatgcaatg
     1201 agtgaagctg cgccaaatac agattttatc atttataata ttccacaatt ggcaggggtt
     1261 gcgttgactg gtagtttgta tgcaacaatg cgtcaaaatc ctcgtgtgat tggagttaaa
     1321 aattcttcta tgcctgtaca agatattcaa atgtttgtag ctgcaggtgg agaagattac
     1381 attgtattca atggtccaga tgaacaatat cttggtggtc gcttgatggg agcagaagct
     1441 ggtattggtg gtacttatgg cgttatgcca gatttgttct tgaaattgga aagtttgatt
     1501 caagaacgag atttagatac tgctaaaaaa cttcaatatg ctatcaatga agttatctat
     1561 aagatgatat caggtaaggc aaatatgtat gctgtagcaa aagaggtttt gcgtctaaat
     1621 gaaaaacttg atttaggttc tgttcgtcaa cccttagaag cattggcaga aggtgacttg
     1681 gaagttgcaa aacaagcagc agaacttatt caacaagcac gaaaagaatt tttataataa
     1741 ataaaatcaa tatggagata tgttatgggc acaacaggat ttacaataat tgacttaatt
     1801 atcttgattg tttatttact tgcggtgttg gttgcaggta tctatttctc taaaaaagag
     1861 atgaaaggaa aagagttctt taaaggagat ggttcggttc cttggtatgt tacttcggta
     1921 tccatttttg ccacaatgct cagtccgatt tccttcttgg gactcgctgg taactcttat
     1981 gcaggtagct ggattttatg gtttgctcaa ttagggatgg ttgtagctat tccactgaca
     2041 attcgtttta tcttacctat ctttgcacgg atagacatcg atacggcata tgattacttg
     2101 gataaacgtt ttaattctaa agcacttcgt attatttcag cactcttgtt tattatttat
     2161 caattgggac gtatgtctat cattatgtac ctcccatcag ctggtttatc agtattgaca
     2221 ggaattgaca tcaatatttt gattattttg atgggtgcaa ttgcaattgt ttattcttat
     2281 actggtggtc taaaatccgt attatggaca gactttattc aaggtgtggt tctgattagt
     2341 ggtgtcgttg tagctttatt tgtactgatg gctaatatta aaggtggctt tggtgcagta
     2401 gcagaaacat tagcaaacgg gaaattcctt gctgcaaatg aaaaactttt cgatcctaac
     2461 ttgctttcaa actccatctt cttaattgtg atgggttcag gctttacaat cttgtcttcc
     2521 tatgcttcat ctcaagattt ggttcaacgt tttactacaa cacaaaatat taagaaactt
     2581 aataaaatgt tgttcacaaa tggtgtcttg tcacttgcaa ttgcaacagt cttttacttg
     2641 atcggtacag gcttgtacgt attctatcaa gtacaaaatg caggtagtgc agctagcaat
     2701 atccctcaag accaaatctt tatgtacttt attgcatacc agttaccagt aggtgtcaca
     2761 ggtttgatct tggcagcgat ttatgcagca gctcaatcaa ctgtttcaac aggtttgaac
     2821 tctgttgcaa cttcatggac attggatatt caagatgtca tttctaaaaa tatgtcagag
     2881 gatcgtcgta cgaaaattgc acaattcgta tctctagcag taggtttatt ctcaattgtt
     2941 gtttccatta tcatggctca ttcagatatt aaatctgcat acgaatggtt caatagtttc
     3001 atgggacttg tacttggtct acttggtggt gtattcattc ttggttttgt ttctaaaaaa
     3061 gcaaataaac aaggtgctta tgcagcgctg attgtatcaa ccatcgttat ggtatttatt
     3121 aaatacttcc ttcctccaac agctgttagc tactgggcat attcattgat ttcaatttct
     3181 gtatcagtag tttcaggtta tattgtatct gttcttactg aaaataaagt atctgcacct
     3241 aaatatacaa cgattcatga tatttcagaa attaaagcgg attcaagttg ggaagttcgt
     3301 cactagtcac taatagaaaa gagaaaataa tatgattatt tctgaacaat ctgattttag
     3361 acgatatgct tctgtcaata aacatttttc aaaagtctgt gattttttag aaaatacaaa
     3421 tttgacagat ttagttgatg gaaaaattga tattgacggg gaaaatgttt ttgcaaattg
     3481 tatgacttat ctagctgatg gagttccagg agatattttt gaaactcata agaaatattt
     3541 agatattcat attgttgttg aaaatacaga aaaaatggca gtaacttccc cagttcgtgc
     3601 tcagtcacgt gtacctttta gcgaagagaa ggatattgct ttttacgata gcaaagacta
     3661 tcaaattgtt gaattgcttc cagggaatat gttggtgact tttgaagaag atcttcacca
     3721 accaaagatt cattgcaatg atgaaactgt taaaaaactt gttatcaaag ttttaaacga
     3781 ggaaaaataa aatgaaaaaa aatattaaac aatatgtaac cttaggtact gtagtggtat
     3841 tatcagcatt tgttgctaac tcagttgcag ctcaggagac tgaaacttct gaagtatcaa
     3901 caccagagtt ggtgcaacct gttgcaccaa cgacttcgat ttcggaagta caacataaat
     3961 cgggtaactc ttcggaagtt actgtacaac ctcgaacagt tgaaactact gttaaggatc
     4021 catcttctac agcggaagaa actcctgtct tagaaaaaaa taatgttact ttaacagggg
     4081 gcggagaaaa tgttactaat gagttaaagg ataaatttac tagcggtgac tttactgtag
     4141 tgattaagta caatcagtct agtgagaaag gtttacaagc tctgtttgga atatctaatt
     4201 ctaaacccgg tcaacaaaat agttatgtag atgtgttcct tagagacaat ggtgaattgg
     4261 gaatggaagc gcgtgatact tcttccaata aaaatcacct agtaaccaga cctgcttcag
     4321 tttggggtaa gtacaaacaa gaggccgtga cgaacactgt tgcagtagta gcagattcag
     4381 tcaaaaaaac gtattcttta tatgcaaatg gtacaaaagt agtagaaaag aaagtggata
     4441 acttcctaaa catcaaggat attaaaggta ttgattacta tatgcttggg ggagtgaaac
     4501 gtgcaggaaa aacggcgttt ggttttaacg gaacactaga aaatatcaaa ttctttaata
     4561 gtgcattgga tgaagaaact gttaaaaaga tgacaacaaa cgctgttact ggacatttaa
     4621 tttatacggc taatgataca acaggttcta actatttccg tattccagtt ctgtatactt
     4681 ttagcaatgg tcgggttttt tcaagcattg acgctcgtta cggtggaact catgatttct
     4741 tgaataaaat taatattgct acaagttata gtgatgataa tggtaagaca tggactaaac
     4801 caaaattaac attagcattc gatgattttg cgccagcacc attagaatgg cctcgtgatg
     4861 ttggtggacg tgacttacaa atcagcggtg gtgcaaccta tattgactct gttattgttg
     4921 aaaaaaataa caaacaagta ctcatgtttg ctgatgttat gcctgctgga gtaagtttta
     4981 gagaagctac tagaaaagat tcaggttata aacaaattga tggtaattat taccttaaat
     5041 taaagaaaca aggtgatact gattataatt atactattcg tgagcatggt actgtatacg
     5101 acgatcgtac caacagacca actgaatttt cagtagataa aaatttcggt attaaacaaa
     5161 atggtaatta tttgacggta gagcaatatt cggtttcatt tgaaaataat aaaaagactg
     5221 aatatcgtaa tgggactcaa gtccatatga atatcttcta caaagatgca ttattcaaag
     5281 tagtgccaac gaactatatt gcatatattt ctagcaatga tcatggcgaa tcttggtctg
     5341 caccaacttt attacctcct ataatgggac ttaatcggaa tgcgccttat ttgggtcctg
     5401 gacgtggaat cattgaaagc tcaactggac gtattcttat tccgtcttac actggtaaag
     5461 agtctgcatt catttatagt gacgataatg gagcgtcttg gaaagttaaa gttgtgccac
     5521 ttccttctag ttggtcagca gaagcacaat ttgtagaatt gagtccagga gtaattcaag
     5581 catacatgcg tacaaataat ggtaaaattg catatttaac aagtaaagat gcaggtacta
     5641 cttggagtgc gccggaatat ttgaaatttg tttcaaatcc aagttatgga acacaattat
     5701 caatcatcaa ttatagtcaa ttgattgatg gtaaaaaggc tgtcatttta agtactccaa
     5761 actccacaaa tggtcgtaaa cacggacaaa tttcgattgg tctaattaat gatgataata
     5821 caattgattg gcgttatcat cacgatgttg attatagtaa ctatggatac tcatattcaa
     5881 cattgacaga gttaccaaat catgaaattg gattgatgtt tgaaaaattt gattcatggt
     5941 ctcgtaatga acttcatatg aaaaatgttg taccatatat aacatttaag attgaagatt
     6001 tgaaaaagaa ttaaagctga aatttaaaaa tatatagaaa gaggataaaa attatggtaa
     6061 attacggtat tgttggagct ggatattttg gagctgattt ggctcgctca atgaacaaaa
     6121 ttgaagatgc aaaagtggtt gcggtatttg acccaaatca tggagaagaa gttgctcaag
     6181 agttgggatc agatgtttgt gcaagtttag atgaacttgt agcacgtgaa gatattgatt
     6241 gtgtgatcgt agcttcacct agctaccttc accgtgaacc tgttgtgaaa gctgctcaac
     6301 atggcaaaca cgtattttgt gaaaagccaa ttgcattgtc ttatgaagat tgtaaagcca
     6361 tggttgacgc atgtaaagaa aataatgtta tctttatggc tggtcacatc atgaacttct
     6421 ttaatggtgt acaccatgct aaagaattga ttactcaagg taaaatcggt aaagttcttt
     6481 attgccatgc cgctcgtaca ggttgggaag aacaacaacc aactgtatca tggaagaaac
     6541 ttcgttctca atctggagga catttgtacc accatattca tgaattagat tgcattcagt
     6601 tcatcatggg aggacttcct gaaaaagcga caatggtagg aggcaatgta tatcataaag
     6661 gtgaaaactt tggtgatgaa gatgatatgc tcattgtaaa cctagaatac tctgatgatc
     6721 gttatgctgt tttggaatat ggtaatgctt tccgttgggg tgaacactac gtcttgattc
     6781 aaggaactga aggagctatc aaacttgact tgttcaatac tggaggtact cttcgtgtta
     6841 aaggtgaagg agaatcacac ttcttagtac atgaaactca agaggaagat gatgaccgta
     6901 cagctatcta taccggtcgt ggtatggatg gagcaattgc gtacggtaaa ccaggagtac
     6961 gttgcccatt atggttacaa acatgtattg ataaagaaat ggaatatcta catgacatca
     7021 ttaaaggtgg agaaattaca gaagaatatg aaaaacttct caatggtgta gctgctttag
     7081 aatcaatcgc taccgctgat gcatgtactt tatcagttaa agaagatcga aaagtaagtc
     7141 tttcagaaat cacaaatgct taactattgt aaaacagaat agtaaattct tgtcattata
     7201 taatttctaa agttctgtga tacaactcat tgaataaaga aatagagatg ggactgggat
     7261 aatgcccagt cccatttttt atcaaaaagt aatgagatca aaaatgtggg agtgatgaaa
     7321 tgaagattat agggatcgat attggcggaa caacaattaa ggcagattta tacgatgatt
     7381 ttggaacgag tttaaatcag ttcaaagaga tagaaacaat tattgactat gatttgggaa
     7441 cgaaccagat attaaatcag gtctgtgatt taattggtga gtatgtttta aattattcaa
     7501 ttgatggtgt tgggatttcc actgctggag ttgttaatgc taatacagga gaaatcatct
     7561 atgcaggcta tacaatacca gggtatatcg gagtaaactt tacttccgaa atagaaaaac
     7621 gttttgggtt gtctactttt gttgaaaatg atgttaattg tgctgcatta ggtgaattgt
     7681 gggagggaca agccaaagat aagaaaaatg tagtaatggt tactattgga acaggtatag
     7741 gaggcagtat tattgtcaat ggacaaattg ttaacggatt taactatact gctggtgaag
     7801 taggctatat tcctgtaggt aattcggatt ggcaaagcaa agcctcaaca accgcattga
     7861 ttcatttata tcaaaagaag agcttgaaaa ctaatcaaac tggacgtact ttctttactg
     7921 atttaagttc tggagataaa gtcgctaaag aaacttttga aatttttgta gaaaatctaa
     7981 caaaaggttt attaactatt tcttatctac ttaatccaga aattctcata ttaggaggtg
     8041 ggattctggc taaaaaggat attttgttac ctgaaattca aagttcttta gctaaaaatg
     8101 caatggataa taggtttttg cctaaaaatg ttgtggcagc tacattagga aatgaagctg
     8161 gtcgtatagg agctgtaaaa aatttcttag atagaatttc taataaatag tgtgaaagat
     8221 aaggaggtgt cacaatgact aactctgtat tttcgacaat gcaagatatt gagaatgttg
     8281 caaccgatat tataaaatca tatgataatg agatttatac gtataaagct gtctcccaag
     8341 aagaattgga aaaactagaa aaaagttatg atgaaaaaag tcacgaagaa ttaatttcaa
     8401 tagaaagcaa tttagaaatg aaacaacaga acctcattga tgaggttaat aaaacaatca
     8461 aggaaaatga tgcaaagatt cagtatattt catcaagtag gagaggagaa tttgtagaaa
     8521 aaattattgg tagggtggta gaaaaatatg gctattagtc agatgaaaag aatttctcta
     8581 ctatttacta agagtagtct tgatgatgtt ttaaaaacta ttcaagaact agagtcagtg
     8641 cagttccgtg atttaaaggt tcaggataac tggtcagaag cgctagaaaa agatgaggtt
     8701 gtattttcaa ctattcaaat ttctcacact tctaattccc atcatggggt tattgaagga
     8761 aatgatgcct tgaattattt gatgaataaa caacaacatt tggaagcaac tgtagagaaa
     8821 ttacaagaat acctaccgaa agaaaacacg tttaagttat tacagcaacc tccgataaca
     8881 acctcttatg aagaattaga aaaatttggt aaagctaatg ttgctgaggg tgttcttaaa
     8941 aaagtgaatc atcaaataaa cagagttcat gaattagaaa gaaacattca aagtaataat
     9001 gaggaaatag agagattaat aaagtgggaa aaattagaaa ttattcctgc gcatttagaa
     9061 caattttctt tctgtagagg aaaagtcgga acaattccaa ggactgtaga taatcgctta
     9121 tacaataatt ttttagaaaa caatattgaa gttcaagaaa tattttctaa tgatagagag
     9181 tacggtgttg ttgttttcta tcagtccagt tgctctattg attttgatga atacttattt
     9241 gaaccatttg attattctag aaaggaatta ccgaagcagc gagtagtaga tttggatcaa
     9301 gaaaacatgc agttaataac tgaaaaagag aatattatcg catcgttgca agattcaaag
     9361 aaaaatttga tagatttaca atggcaaatt gactatattt tatctatcta tgctcgtcaa
     9421 atctctaaga ataacctttt gagcactccg catctagttg cattagaagg atggatagaa
     9481 gaaactcgta ttttatattt tataaaagtt atggatgagc gttttggaca ttctatttat
     9541 atttatgaat cggaaacatt gtcggataat caagatgaaa tacctatcaa attaacgaat
     9601 cactctttaa ttgaaccatt tgaattattg acagaaatgt atgctctgcc caaatattat
     9661 gagaaagatc ctacacctgt attagcacca ttttacttta cattttttgg aatgatggtt
     9721 gctgatttag gctatggttt actattgttt ttaggaataa tgttagcatt aaaaattttt
     9781 catctacctt cagtaactaa gaaattttta aaattcttta atatattagg ggtagccgtt
     9841 gcaatttggg gtggaatcta tggctcattt tttggatatg agttgccatt tcatctgata
     9901 actacaacct cggatgtcat gactatatta gtagtgtcag ttgtgtttgg atttattaca
     9961 gtatttgtag gtttgttagc ttcaggacta caaaaagtaa gaatgaagaa atatgcagaa
    10021 gcatataatt caggatttgc gtggtgtgtt attctgcttg gcttgttatt tattgctgtc
    10081 ggaatgttga tgcctgatat tagaccgtta tttgtattag ggaaatgggt atctattttt
    10141 aatgctgtgg ggattttgat tgcttctatt attcaagcca aaagcttgtc aggtattggc
    10201 tcaggattgt ttaatctata taacatttca tcttatatag gtgatttagt tagtttcact
    10261 cgattgatgg cattaggatt atctggagca agtatagcat cagctttcaa tttaattgtt
    10321 ggtttgtttc cggggacatt ggctaaactg acaattggat tagtattatt cattctttta
    10381 catgcgatca atatttttct atcgttacta tcaggatatg ttcatggagc gcgtctgata
    10441 tttgttgaat tttttggtaa gttttatgag ggtggaggaa aaccatttca acctttgaag
    10501 gcttctgaga aatatattaa ggttattaca aagaattaat ggaggatata tataatggaa
    10561 catttagcaa cttatttttc aacctatgga ggagctttct tcgctgcatt gggaattgta
    10621 ttggcggttg gattaagcgg tatggggtct gcttatggag ttggtaaggc tgggcaatct
    10681 gccgcagctt tactgaaaga acagccagaa aaatttgcct cagctttgat attgcaacta
    10741 ttgcccggaa cacaaggatt atatggtttt gttattggaa ttttgatttg gttgcaatta
    10801 actccagaac ttcctttgga aaaaggcgtt gcttatttct ttgtagctct tccaattgct
    10861 attgtaggat acttttcggc taagcatcaa ggaaatgtag cagtagcggg aatgcaaatc
    10921 ttggctaaaa gaccaaaaga attcatgaag ggagcaattt tggctgccat ggtagaaacc
    10981 tatgcaattc ttgcctttgt cgtatcattc attttgaccc ttcgtgtata agaaattaat
    11041 ttgctattca aaggaggtgt ctaaatgagc aatttagaaa acttacgaga atctgttatt
    11101 gaacaagctc atgaaaaagg gagtatgaaa ttattggatt ccaaaaagaa gattgatgat
    11161 gaatttgaaa tgcaaaagtc ggtcattgta aagaaaaaag aagctgaaca tgaacgaaag
    11221 ttaaaagaat tacaacagaa atatcaaata atttttcaac aattaaaaaa taaggaacgc
    11281 caatcaacgt tagtatcaaa acagaaaata ttaaaagaac tttttcaatc tgctttaata
    11341 gaaatggaat cttggagtgc agataaagaa atggagttca tctatcgaat tctggaacga
    11401 tattcacaac aagaggtcat agtaactttt ggggagcgga ctttagctaa attcaatttg
    11461 gaacaattag agaaattgaa attctctttt ccaaatcatt tatttagtga acagcctatc
    11521 tcaaatgaat caggcttact tatttcaata ggtaaaattg atgataacta tttgtataaa
    11581 acgttaattg gatcgatttc taaggaagaa agttcaagta tcgcaaatca aatttttatc
    11641 aat
//
LOCUS       DAVKFH010000059        11609 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKFH010000059 DAVKFH010000000
VERSION     DAVKFH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.059"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VW006_001691"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VW006_001691"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9380507.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VW006_001692"
     CDS             complement(1169..1693)
                     /locus_tag="VW006_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9380508.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VW006_001693"
     CDS             complement(1746..1982)
                     /locus_tag="VW006_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9380509.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VW006_001694"
     CDS             complement(2050..2970)
                     /locus_tag="VW006_001694"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9380510.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3161..3564
                     /locus_tag="VW006_001695"
                     /pseudo
     CDS             3161..3564
                     /locus_tag="VW006_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3780..4361
                     /locus_tag="VW006_001696"
     CDS             3780..4361
                     /locus_tag="VW006_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9380511.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4358..5635
                     /locus_tag="VW006_001697"
     CDS             4358..5635
                     /locus_tag="VW006_001697"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9380512.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5653..6315
                     /gene="deoC"
                     /locus_tag="VW006_001698"
     CDS             5653..6315
                     /gene="deoC"
                     /locus_tag="VW006_001698"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9380513.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6302..6691
                     /locus_tag="VW006_001699"
     CDS             6302..6691
                     /locus_tag="VW006_001699"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9380514.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6782..7834
                     /locus_tag="VW006_001700"
     CDS             6782..7834
                     /locus_tag="VW006_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9380515.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7978..9513
                     /locus_tag="VW006_001701"
     CDS             7978..9513
                     /locus_tag="VW006_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380516.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGAINADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            9506..10564
                     /locus_tag="VW006_001702"
     CDS             9506..10564
                     /locus_tag="VW006_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380517.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10567..11523
                     /locus_tag="VW006_001703"
     CDS             10567..11523
                     /locus_tag="VW006_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380518.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3308 a         2077 c         2656 g         3568 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttgatgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctctctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgtgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agttgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc cacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc atcttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ctactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttaattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctt ttcgggtacg atgtcgtaaa cttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatatag
     3061 tataagtgat caatttattg atgaaatagg agatagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttagtag gaggtgtact atgaaaatct taaaacgtta
     3181 tatattggaa ctctgtttta ttttaagttt tgctttacct tttataaaag gaaccaatgc
     3241 agataatggt agatgctttg tggaaaccta ttacggtttt acttttttga tggaacatgc
     3301 tattgtaaca gctgtcttta tctgttcgtt cttaattgct ttcttactaa aaaacgatgg
     3361 acgaaatgga ttgctgcggg tagttattgc tttttagtcc tacggattgc gacagagggt
     3421 tatttcttcc acatgtcact agaagatttg atacgacttt ggacaagttt ggaaatcctg
     3481 acacaaacgt atcagttggg cttttatcta aacattttgt taggaatttt gttgataata
     3541 aagtatttta aggttaagca atagaaataa taatctgcct gattatagat gataaacttt
     3601 ttgtttttat ctggactgtg atatttatag ttctaaatgg tttattattt ataagtgttg
     3661 aaagagattg aagaaaatgg cttcgtgact tgctttcttc ggtctttttc gttttattat
     3721 caatttgtaa acgatttaca atttgagtca aattatggta aaatagtgtc atgagtaaaa
     3781 tgtactatgc agaaaatcct gacgctgctc acgacattca tgagttgaga gtggacttgt
     3841 tgggagaaaa aatgactttt ttgacagatg cgggtgtttt tagcaagaaa atggttgact
     3901 ttggaagtca actcttgctc aagtgtctgg atgtcaacca aggagagaca gtcctagatg
     3961 taggctgtgg ttatggtcca ttaggtttgt ccttggtcaa ggtttacgga gttcaggcga
     4021 ccatggttga tattaacact cgtgccctgg atttagctca gagaaatgct gaaaaaaata
     4081 atgcaaaagc gacgatattt caatctaata tttatgcaag agttcaaggg cgatttgacc
     4141 atgtcatttc caatcctcct atccgtgcgg gcaagcaagt cgttcatgaa atcatcgaga
     4201 aaagtaaaga tttcttggaa attggtggag atttgaccat cgtcatccag aaaaaacaag
     4261 gagctccaag tgccaaatct aagatggaag atgtgtttgg caattgtgaa atccttaaaa
     4321 aagacaaggg atactatatc cttagaagtg tgaaagaatg agagcagttg atttaatcca
     4381 aaaaaaacga gacggtcaag aactgacttc aagtgaaatt gaatggctga tagaaggcta
     4441 tgtatcagga actgttcctg attatcagat gtctgccttt gctatggctg tttattttaa
     4501 aggaatgacg actcgagaaa tctctgattt aacgatgaat atggttaaga ctgggcaaga
     4561 gtttgacctg tcagccattg atggtgtgaa agttgacaag cattctactg gtggtgtcgg
     4621 cgataaggta accttgattt tagctcctct tgttgctagc ttcggtgtgc ctgtagcaaa
     4681 aatgagtggt cgtggtcttg gccatacagg cggaacaatt gataaattgg agtccattag
     4741 gggctatcaa gtagaacgta gtcaagagga ttttattcgt caggttcagg atattggagt
     4801 atctgtcatt gggcaatcag accagctggt caaagcagat aagcttctct atgcccttcg
     4861 tgatgtgacc gcaactgtcg acacgattcc tttgattgcg agttcggtga tgagcaagaa
     4921 aattgcggca ggggcggatg ctattttgct agacgtgact gtcggtgagg gtgccttcat
     4981 gaagacggtt gatgaggcgc gtgagttggc tcaaactatg gtagatcttg gtaaggttgt
     5041 tggacggaag acagtagcag tgattacgga tatgagccag cctttggggc gagcgattgg
     5101 aaatcgtctg gaaatccttg aagcattgga gattttgcaa ggtcaaggac gtcaggatat
     5161 tacccacttt atttgtgaat tggctcaaat tatgcttggt ttggcaaatg ttaacaaaac
     5221 tgttgaagaa gttcgccaac atcttgaaaa tggtcaagca ctggctaagt ttgaggaaat
     5281 ggtgcaagcc caaggtggag acttggaaga cctctatcgt cctgtaaatg tagctcatgt
     5341 ggtggaaatc cctgctcagg aaacgggtgt catttcagct cttccagcta tggactttgg
     5401 tctttatgcc atgaggctgg gtgctggtcg tgcagttaag tctgatgtct tggactatga
     5461 aacaggaatt gtttttgaaa agaagattgg agactccgtt caaaagggtg aaattgttgc
     5521 aaaagtatac acaaatgaaa aaattccacc tcaactagtt acagatttcc aaaaatgtgt
     5581 taaaataagt gatgaagtga aaaaaatccg tgaaattgta gaaatcatct cctaaatgga
     5641 gggaaaaccg aaatgaaatt aaataaatat atagatcata cgcttttaaa acaagatgca
     5701 aagaaaaaac aaattgatag tttgttgtct gaggctagag agtatggctt tgccagtgtt
     5761 tgcgttaatc cgacctgggt tgaacatgct aaaaaaggac ttgaaggcac agatgttaag
     5821 gtttgcacag tagtaggttt ccccttggga gcaacaactt caactgtgaa agcttttgaa
     5881 acaaaagaag ctatccaaaa tggggctgat gagattgaca tggtaatcaa tgttggggct
     5941 ctcaagtcag gtgatttggc cttggttgag tcggatattc gtgcagtagt ggaagcaagt
     6001 ggggacaagt tagtgaaagt tattattgaa gcttgccttc tgacagacca agaaaaagtt
     6061 ttggcttgcc aattagccca aaaagcagga gctgactttg tcaaaacatc tactggcttt
     6121 tcaactggtg gtgctacgat agcagatgtt agattaatgc gtgaaacagt tggacctgat
     6181 atgggtgtca aggccgccgg tggagctcgt tcttatgcag atgctcttac ctttgtcgaa
     6241 gcaggtgcga cccgtatcgg aacgtcagct ggggtagcta ttttaaaagg agaattggca
     6301 gatggcgact actgagttga ttgaactggc aattgaaacc agcaaacatg cctatgtccc
     6361 ctattctcac tttcctatcg gagcagtttt agtagccaaa gacggaagtg tttacacggg
     6421 agtgaatatc gaaaatgcta gctatccttt gaccaattgc ggtgagagaa cagctatttt
     6481 taaggctata tctgaaggcc aaagagaatt ttcagaattg attgtctatg gtcaaactga
     6541 aaaaccgatt tcaccatgtg gtgcttgtcg ccaagtaatg gtcgaatttt ttgaacaaga
     6601 tttaaaagtg accctagtcg caaaagataa atcgacggtc gagatgacgg tcggggagtt
     6661 acttccatac tcttttacag acttaaatta gtctgagtcg ctctttgagt ggcaagggtc
     6721 tttgtgacca atcaatccat acttgcaaca tcgttgcaca tcttatttag gaggttcagt
     6781 aatgaacaag aaacaatggc taggtcttgg cctagttgca gtggcagcag ttggacttgc
     6841 tgcatgtggt aaccgctctt ctcgtaacgc agcttcatct tctgatgtga agacaaaagc
     6901 agcaatcgtc actgatactg gtggtgttga tgacaaatca ttcaaccaat cagcttggga
     6961 aggtttgcaa gactggggta aagaacacaa tctttcaaaa gataacggtt tcacttactt
     7021 ccaatcaaca agtgaagctg actacgctaa caacttgcaa caagcggctg gaagttacaa
     7081 cctaatcttt ggtgttggtt ttgcccttca caatgcagtt gaagaagcag ctaaagaaca
     7141 cactgacttg aactatgtct tgattgatga tgtgattaaa gaccaaaaaa atgttgcgag
     7201 cgtaactttc gctgataatg agtcaggtta ccttgcaggt gtggctgcag caaaaacaac
     7261 taagacaaaa caagttggtt ttgtaggtgg tatcgaatct gaagttatct ctcgttttga
     7321 ggcaggattc aaggctggtg ttgcgtcagt agacccatct atcaaagtac aagttgacta
     7381 cgctggttca tttggtgatg cggctaaagg taaaacaatt gcagccgcac aatacgcagc
     7441 cggtgcagat attgtttacc aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc
     7501 aaaatctctc aacgaaagcc gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgaccg
     7561 tgaccaagaa gcagaaggta aatacacttc taaagatggc aaagaatcaa actttgttct
     7621 tgtatctact ttgaaacaag ttggtacaac tgtaaaagat atttctaaca aggcagaaaa
     7681 aggagaattc cctggcggtc aagtgatcgt ttactcattg aaggataaag gggttgactt
     7741 ggcagtaaca aacctttcag aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat
     7801 ccttgatgga agcgtaaaag ttcctgaaaa ataatggatg gaagtcattt cttaggaagc
     7861 ggccaagggc cgctttttta agagttgaga caaagtcatt ttgtctcagt aaagcttgct
     7921 actagacaaa ctagcaagtt ttattgaaat aaaataagac tctgaaagga agagcacatg
     7981 gcacacgaaa atgtcattga gatgcgtgat attaccaagg tgtttggtgg atttgttgcc
     8041 aacgacaaaa tcaacttgca cctacgaaaa ggtgaaattc atgcactttt aggagaaaat
     8101 ggggctggta agtccacgct aatgaacatg ttagcaggcc ttcttgaacc aaccagtggt
     8161 gaaatcgcgg tcaacggtca agttgtcaat ctcgactccc catctaaagc agctagcttg
     8221 ggaatcggga tggttcacca gcactttatg ttggttgaag ccttcacagt ggctgaaaac
     8281 atcattttag gtagtgaatt gactaaaaat ggtgtgctag atatcgctgg agctagcaaa
     8341 gaaatcaagg ctctttctga acgttatggc ttagctgttg acccttctgc caaggtagca
     8401 gatatctcag ttggagccca acaacgtgta gaaattttaa aaacacttta tcggggggct
     8461 gatatcctta tctttgacga accaacggct gttttgactc catcagaaat tgatgagttg
     8521 atggctatta tgaaaaatct tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa
     8581 ttggatgaaa ttcgcgcagt ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt
     8641 gaaaccgttg aaattgcagg ggctatcaat gctgatttgg cggaaatgat ggtaggacgt
     8701 tctgtttcct ttaaaacaga gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc
     8761 aaagatttgg tggtcaatga aaaccgtggt gttccagctg ttaaaaatct gtccttggat
     8821 gttcgtgctg gagagattgt tggtattgcg gggattgatg gaaatggtca gtctgaactg
     8881 attcaagcca ttacaggtct tcgtaaggtt gaatctggta gcattgagct aaaaggagat
     8941 tcaattgtag gcttgcaccc acgtcagatt acagaactaa gtgttgggca cgttccagaa
     9001 gaccgtcacc gtgatggctt gattttggaa atgatgatat ctgaaaatat tgcccttcaa
     9061 acctactata aagaaccaca tagtaaaaat ggaattttga attattcaaa tattacttct
     9121 tatgctaaaa agctgatgga agagtttgat gttcgcgctg ccagtgaatt agttcctgca
     9181 gctgcactct caggaggaaa tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat
     9241 cctgatctcc ttatcgttag ccagccaact cgtgggttgg atgtcggtgc cattgagtat
     9301 atccacaaac gcttgattga agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt
     9361 gaattggatg agattttaaa cgtctcagac cgtattgccg ttatccacga tggtaagatt
     9421 caaggtattg tatcaccaga aacaaccaat aaacaaggac ttggtgtctt gatggctggt
     9481 ggaaacttgg gaaaggagaa gagtgatgtc taaaaaatta caacaaattt cggttccctt
     9541 gatttctgta ttcctaggaa ttttactcgg agccattgtc atgtggatct tcggttatga
     9601 tgctatttgg ggctacgaag aattgttcta tacagccttt ggcagtctgc gtgggattgg
     9661 agaaatcttc cgtgctatgg gtcctctggt cttgattggt cttggttttg cggttgccag
     9721 tcgagctggt ttctttaacg tcggacttcc tggtcaggct ttggcaggtt ggattctcag
     9781 tggttggttt gccctgtcgc atccagatat gccccgtccc ttgatgattc tagcaaccat
     9841 cgtgattgcc ttgattgctg gtgggattgt cggagcgatt ccaggtattc ttagggccta
     9901 tctagggacg tcagaggtta ttgtaaccat catgatgaac tacattgtct tgtatgtagg
     9961 gaatgccttt atccatgctt tccctaaaga cttcatgcaa agtacagatt cgaccattcg
    10021 tgttggggct aatgcaacct atcagacacc ttggttagct gagttgactg gtaactcacg
    10081 gatgaatatt ggtattttct ttgccatcat tgccgttgca gttatttggt tcatgctcaa
    10141 gaaaacaact cttggttttg aaatccgtgc agttggtctt aatccacatg cttcagaata
    10201 tgctggtatt tctgccaagc ggactattat cctatctatg attatttcag gtgccttggc
    10261 aggtcttggt ggagctgttg aaggtttggg aaccttccag aacgtctatg ttcaaggttc
    10321 gtcattagct atcggattta acggaatggc ggttagtttg cttgcggcca actcaccaat
    10381 tggtatactc tttgcagcct tcctatttgg cgttctccaa gttggggctc ctggtatgaa
    10441 tgcggcgcag gtaccatctg agctcgtcag cattgtaaca gcgtctatta tcttctttgt
    10501 cagtgttcat taccttatcg aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa
    10561 gtaaagatgt ctattataac cttgctcccc ctcttggtgt cttctatgct gatttactca
    10621 gcacctctca tctttacaag tatcggtggt gttttctctg aacgtggtgg tgtggtaaac
    10681 gttggccttg aaggaattat ggttatgggt gccttttctg gagttgtctt taaccttgaa
    10741 tttgcagaac aatttggagc agcaactcca tggctatcct tgcttgtagc aggattggtt
    10801 ggtagtgttt tctctatcat ccacgcagca gcgacggttc atttccgtgc agaccatgtt
    10861 gtcagcggta cggtattgaa cttgatggcg cctgccttgg ctgttttctt agttaaagtt
    10921 ctttataaca aaggacaaac cgacaaccta agtcaaactt ttggacgctt tgatttccca
    10981 gtcttggcaa atatcccagt gattggtgat atcttcttca agtcaactag tctacttggt
    11041 tatctggcga ttgccttctc attccttgct tggtttattc tctttaagac tcaatttggt
    11101 cttcgtctcc gctctgtcgg tgaacatcct caagcagcgg ataccttggg aatcaacgtc
    11161 tacaagatga gatatttagg ggttattatt tcaggttttc taggtggaat tggcggagcg
    11221 atttatgcgc aatcaatctc agttaacttc tcagtgacaa ctattgttgg acctggattt
    11281 atcgcccttg ctgcgatgat ctttgggaaa tggaatccaa tcggtgctat gctttctagt
    11341 ctcttctttg gactttcaca aagtttggct gttatcggtt ctcaattgcc gttcctacaa
    11401 ggagtgccag cggtttatct tcaaattgca ccttatgttt tgacaattct tgtcttagca
    11461 gccttctttg gaaaagcagt cgcaccaaaa gcagatggta tcaactacat caaatcaaaa
    11521 taagcataca aaaaaacgtc agtccaatga ctgaactgcc ccccaaaagt tagacagaaa
    11581 aaatctaact tttggggtgt ttttattat
//
LOCUS       DAVKFH010000060        11572 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKFH010000060 DAVKFH010000000
VERSION     DAVKFH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.060"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(143..994)
                     /gene="rgg"
                     /locus_tag="VW006_001704"
     CDS             complement(143..994)
                     /gene="rgg"
                     /locus_tag="VW006_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9380519.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNVAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFYTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            1118..1459
                     /locus_tag="VW006_001705"
     CDS             1118..1459
                     /locus_tag="VW006_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9380520.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(1668..3371)
                     /gene="ilvD"
                     /locus_tag="VW006_001706"
     CDS             complement(1668..3371)
                     /gene="ilvD"
                     /locus_tag="VW006_001706"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9380521.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(3604..4536)
                     /locus_tag="VW006_001707"
     CDS             complement(3604..4536)
                     /locus_tag="VW006_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9380522.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(4533..5390)
                     /locus_tag="VW006_001708"
     CDS             complement(4533..5390)
                     /locus_tag="VW006_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9380523.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(5394..6740)
                     /locus_tag="VW006_001709"
     CDS             complement(5394..6740)
                     /locus_tag="VW006_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9380524.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(6753..7037)
                     /locus_tag="VW006_001710"
     CDS             complement(6753..7037)
                     /locus_tag="VW006_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9380525.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(7041..9071)
                     /locus_tag="VW006_001711"
     CDS             complement(7041..9071)
                     /locus_tag="VW006_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9380526.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEYYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            9262..10269
                     /locus_tag="VW006_001712"
     CDS             9262..10269
                     /locus_tag="VW006_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9380527.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            10272..10472
                     /locus_tag="VW006_001713"
     CDS             10272..10472
                     /locus_tag="VW006_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9380528.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            10624..10806
                     /gene="rpmF"
                     /locus_tag="VW006_001714"
     CDS             10624..10806
                     /gene="rpmF"
                     /locus_tag="VW006_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9380529.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            10822..10971
                     /gene="rpmG"
                     /locus_tag="VW006_001715"
     CDS             10822..10971
                     /gene="rpmG"
                     /locus_tag="VW006_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9380530.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(11463..>11572)
                     /locus_tag="VW006_001716"
     CDS             complement(11463..>11572)
                     /locus_tag="VW006_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9380531.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3746 a         2435 c         1900 g         3491 t
ORIGIN      
        1 tatagcactt atgaatttgc gaaacgattc ttttgaatat atgcaacaaa caaaatgagc
       61 aaaaaaacga tagaactttt gtaatgacct gagagagggc aacagttcta tcgcttcaaa
      121 ttttttcttg gtttgcagat actcaacatt cggtcgtatt tctatagtat tcggcagatt
      181 tattacagcc aagcatctca aaaatacgga cagcatcctc catctttttc tgaccttcct
      241 tgactctacc ttgcttgcta tcaaggagac cttctgccca cagataaaca attcggaaat
      301 aggtctcatt ttccttgtag aaatgctctt cgataacacg tttaaaataa taggcattgg
      361 taaattcttc acactcaata ctagctaaaa agccattcaa tagtatagta taaaaaaggt
      421 ttcgattgcc agacatttcc attagaaaat cggatttacg taccatttct cgtacatatc
      481 tagtaaaaag agaaacagat aaaaatggag aactgactga aaataaattg agttcataga
      541 ttccccagat ctcggtagaa aacaaataat catgaaggac ttttccttcc tctgctgtta
      601 actctaccct ttcatccatg ctcttcatat aagacttgat cataatggca tttagaatat
      661 gtttctgttt gttgtgagaa tgggcatgct tttgatactc cctgcgatat aagtcctcaa
      721 gaggtgctac attctttggt tccaagacat ctgtaatttc ttttctcaac tcagaatctg
      781 tatcatactg gaaacctctc gccagaaaga ggatctcctc cacactggca gatatatttt
      841 ccagagcaaa tagaaacttt tccaccgaaa gctcactctg acctgtttca aaacgggaca
      901 acatagacgg cgaaaattgt cctccggttg cttgtctcag tgagatattt cttgactctc
      961 gtaattgtct aaagactttt ccaatctgct ccatagactt ccccttgatt ccgtattttc
     1021 ttcattttat catatttttc agaaaattca tcaaaaactt gccaaattgt cagaattatg
     1081 agaaaataga ggatatttat cacgtggagg gactgctatg agagacgata tcaaaatcaa
     1141 tgaccgtgct ttggccttgc aagaccaaat catcgaaaaa ctagagaaag tttttgatac
     1201 agatgtggaa ttggatgttt acaatctagg tctgatttat gaaatcaatc tggatgaaac
     1261 ggggctctgc aagattgtca tgaccttcac cgatactgcc tgtgattgcg ccgaaagcct
     1321 gcctattgaa atcgtggcag gtctgaaaca aatcgagggt atcaaagata tcaaggttga
     1381 agttacctgg tcgcctgctt ggaaaatcac acgaatcagt cgctatggcc gtattgccct
     1441 tggactacca cctcgttaag caggccaatc atttttaaag atgaaaaaag caagccaaaa
     1501 atggcttgct ttttgatact caatgaaaat caaagggcaa actagaaaac tagccgcagg
     1561 ttgctcaaaa cactgttttg aagttatgga tagaactgac gaagtcagta accataccta
     1621 cggcaaggcg acgttgacgt gatttgaaga gattttcaag tatgagttta ttttttacct
     1681 gacttgtcca tattccagaa gtctgtcacg gctccgcgtg aagcagatga tacgatatgg
     1741 gcatatttac cgaggacacc acggctgtaa agtggtggca aggttgtttc tgccttgcgt
     1801 ttttcaagtt cttcttcgga tacggccatg gaaatttctt tggtatcttg gtcaaccgta
     1861 acgatatcgc cggtacggag ataggcaatt ggtccaccat cctgagcttc aggagcgata
     1921 tgtccaacaa ccagaccata agtaccacca gagaaacgtc cgtccgtcaa gagggccacc
     1981 ttatctccct gacctttacc aacaatcatt gaagaaagtg acagcatctc aggcatacca
     2041 ggaccacctt taggtccaac aaaacgaaca acgactacat cgccatcaac gatttcatct
     2101 gtcagaacgg cctgaatcgc atcttcttct gagtcaaaga ccttagctgg cccaacgtga
     2161 cgacgcactt taacacctga taccttggca actgcaccgt caggagcaag gttcccgttc
     2221 aagatgataa gcggaccatc cgcacgtttt ggattttcaa gtggcatgat aactttttgg
     2281 cctggagtca agtctgcaaa gtcagccaag ttttcagcta cagtcttacc agtacatgtg
     2341 atgcgatctc cgtgaaggaa accatttgcc aacaagtact tcataaccgc agggacacca
     2401 ccgacttcgt agaggtcttg gaagacatac tgaccagatg gtttcaagtc ggccaagtga
     2461 ggcacacgtt cttgaatcgt attgaagtcc tcaagtgaca agtcaacatt tgcggcatgg
     2521 gcaatggcga gcaagtgaag agtggcgttt gtagaaccac cgagagccat cgttacagta
     2581 atagcatctt caaaggcttc acgagtcaag atatctgatg gtttgagacc aagttccaac
     2641 atcttaacaa cagcacgtcc tgctgcttcg atatcttctt tcttatcagc tgattcagct
     2701 gggtgagagg atgaccctgg caaactcatc cctagaactt cgatagcagt tgccatggta
     2761 ttagcagtat acataccacc acaaccacca gggccagggc aggcattaca ttcaagacgt
     2821 ttcacgtcct cagctgtcat gtcaccgtgg ttccattttc cgataccttc aaagacagaa
     2881 accaagtcga tatctttacc atcaagattt cccggtgcaa tagttccacc ataggcgaaa
     2941 atagctggga tatccatatt agcaatagca atcatagatc caggcatgtt cttgtcacag
     3001 ccaccgatag cgacgaaggc atccacgttg tgaccactca tagccgcctc gatggagtcc
     3061 gcgatgatgt cacgagatgt tagagagaaa cgcataccag gcgttcccat agcgatcccg
     3121 tccgctacgg taatggttcc aaactgtaca ggccaagcgc ctgcagattt gacaccttct
     3181 ttagccagtt tcccgaaatc atgcaagtga atgttacatg gtgtattttc cgcccaagtc
     3241 gaaatcactc ccacaatcga tgtttcaaag tccttatctg tcataccagt cgcacgaagc
     3301 atagcacggt taggtgattt aaccatgctg tcatagatgc tactgcggtg acgtttatct
     3361 aattcagtca tcttatccct cccatttcag tttttactat tatagcacaa tttaaaggca
     3421 atgaacagaa taaaattctt gaattttcag aaaattctat acgcatgtga aatatttaaa
     3481 attaaaaaca acaaagcgga ttagtgcact ttctgatgac cagaatatgc tttttaatcc
     3541 gctttcttta aataacgtac tgtaattttt acagaaattc tttcagataa gtgtatttaa
     3601 catctatctt gcattataaa tttctagaac cttctctttt atattcgatt cactcaaacc
     3661 atactcatta agaagataat ccattttccc tacttgaccg aatctttctt gaacacccat
     3721 ccgatgaatt tttgttattc catcatcaga gaataattca cataaagcac tgccaattcc
     3781 acctatctga ttgtggtttt ctacagtaaa tatagttttt ccacttaaca ttgtttttat
     3841 ctgttctggt atcggtttga ttctaaataa atctatcaca cttactgaat aacctaattt
     3901 agacagttca tctgcaactc gaatacttgg agcaaccatt ataccagaag caacgattac
     3961 aagatcttca ccatgcctta actcaatgta gcctttagaa aaatcttctc caccttgata
     4021 cacaggaact ggagcttttc taattgttcg aatatatttt agtcctttta agtctaatgt
     4081 ctggttcaat atttcacgaa attggatatc atcagttgct tcaaaaatga tcgatttagg
     4141 aattaaacgt aacaatccaa tttcttcaaa tggcatatgt gtcccaccat tcatttctgc
     4201 agttactcct gcatctgatc caatcacagt ggcatccaat tgtgcgtatc caagagaaat
     4261 aaataattga tcaaatactc ttcgtgaagc aaaaggacca aatgtatgaa gataaggtct
     4321 aaacccctga atagacaagc ctgctgcaag gccgaccatt tctgcttcca taatcccaac
     4381 attcacataa cggtctccaa agtccttttc aagattatga gtagccatcg aacttgacaa
     4441 atcggcttct aagactacta tatcagaatc actttgatta gcctctaaaa ggaagtctct
     4501 atatacatgc cgtaattctt tcgtgcttct catcattctg tttcctccaa ttcctgactt
     4561 aatctttcta caactgaggt taacatttgt ctctcctcta cagtagggcg aagatgatga
     4621 ttggatttca tttcttccag ctcttgaacc ccttgacctt taatagtatc taatacaatg
     4681 cacttaggtg atgaattatt tgactgtttt aattggacaa tcccttcata aatttctcta
     4741 atatctgaac ccttgaccct aatggattca aatccaaatg ctgaaaattt ttctacgaaa
     4801 tcacctggat tacaaatatc ctttgtaaaa ccatctaatt gttttttgtt atcatcaaca
     4861 aatacaatta agttggataa ctgttgatga gaagcaaact gtatagcctc ccaacattgt
     4921 ccctcattta actcaccatc tccaacaata gcataagtat aaaagggact ctttcttatt
     4981 ctctgaccat atgcaagtcc agttgcaaca ctaattcctt gtcctaaaga gcccgttgtc
     5041 atatctatgc ctggcgttag atttctatca ggatgagacg gtaatttggt tccatttgta
     5101 tttaaagaat ataagaattc tttgtcaaag aaaccattca aatagagtgt actgtataga
     5161 gctggtcctc catgaccttt tgataatatg aaataatctc tatctcgtgc tgcaaatatt
     5221 tctggagtca ttggcattat ttcaccataa agcactgcta aaacttctac gatagacaga
     5281 cttcctccgt aatgtccgaa tccaagatga ttcaatgttc taagagtatt taatcggatg
     5341 ttagtcgcaa attttcttaa cccatcttct ctatttttac ttaaaatcat cccttattcc
     5401 tcctttgcag atggcttttt aataaaggat actccaaaca taactgctag aataagaaca
     5461 agaccaatca caatgcctgc ttgtgagcca aattgattta acattcctaa aataattcct
     5521 gatagaccaa aatctgcatc tgagaaagtt gatccttgga aaccaagtcc tcccaaaact
     5581 ggcattaaaa agactggaag aaaactgatt aaaatacctt gtaaaaatgc tccaatagtg
     5641 gctccacgaa caccaccaga tgcattccca atgacacctg cagtcgctcc acagaagaaa
     5701 tgaggcacaa cacctggtaa gataacaacc gttcctgaag caatcataat tgccatactt
     5761 actaaaccac caataaaact agagataaat ccaattagaa ctgcattggg tgcataagta
     5821 taaacaatcg gacaatccaa agcaggtttt gaattaggta caagacgctc tgaaatacct
     5881 ttaaaggctg gaacaatttc gcccaaaata aggcgaacac ctgctaaaat aacaaatacc
     5941 cctgctgcaa attgacctgc taattgtaaa gcataaacta gaccacttgt accactactg
     6001 atttcttttt ctatatattc tgaccctgca aagatagcta caataatgta aataactgcc
     6061 atggataaag taatactaac agtactatca cgtaaaaaag ctaaactctt tggaaattta
     6121 atgtcctctg ttgattttga tttgtcaccg ataaggctac cagtaaaacc actcaaccaa
     6181 tatcccaaag aactgaaatg acctaaagcc accttgtcat ttccagttaa ttgaaccata
     6241 tatttttgaa caaatgctgg ggaaatactc ataataatac cgagtgctaa tcctcctagt
     6301 aagataagag gcaagctagt aaagccagca actgataaaa tgaccgcaat catacatgcc
     6361 atatatagag tgtggtgccc tgttaaaaaa atatatttaa atcgagtaaa acgagcgatt
     6421 aagatattga acaccatgcc tgcaaacata atcagtgcag tagctgagcc atatgttgtt
     6481 aaagctacag ctacaattgc ttcattattc ggcacaacgc cagataaatg aaaagcatgc
     6541 tcaaacatgg taccaaatgg atttaaagaa ttttgtacaa ttcctgcacc accagataca
     6601 actaagaaac caacaaaggt cttaattcca cctttaataa tatcaggtaa tttcttcttc
     6661 tgaagaacta atcctaaaat tgcaattaaa gctactaaaa tagctggtgt actaacaata
     6721 tccaatatga acttcatcat gacgctagcc tcctatataa gtcctttttc ttcacaaagt
     6781 ttagtaatta attctcgtag ttcatccata tcaataatac tatttaagat acgaacatct
     6841 ccaagatgac tagctgaatc agctagatca cgaccaacaa tccaaatatc agctgcattt
     6901 ggatctgctc cacctaaatc ataatgttca acttctacat ctgaaacatt caaatcactc
     6961 aatacagatt caatattcat ctgtaccata aaacttgaac ctaatcctga accacaagct
     7021 gtaccaattt ttaacattat ctaatcctcc tgtttaatta tcattttaat gtcatcatag
     7081 ttttttgatg atattaaagt ttgaacatga tttttatctc ttaaaattgt tgttaaatgt
     7141 gacaaagcct ttaaatgact ctcattatca atggctgcaa tacaaatcaa caatcttacc
     7201 tcttgttctg gattatccaa taaataaatc ggttcttcca aaactaacat tgacattcct
     7261 atttcattca caccttcatc tggccgagcg tgaggaattg ctactccctt ccctaaatta
     7321 ataaaaggcc caaactcttc tactttttga atcattgcct cagggtagtt ctcagttatc
     7381 ttatcttgat ccaaaagcgg tttagctgct aaacgaatcg cctccttcca tcctaatttt
     7441 tgcgaactaa cctgataggt ttctttggta atgagttgtt ctagcactgg tacaatttcc
     7501 tttctatcat ttttttggta aagataattc tttaacgcca atcttaattc caattcttgt
     7561 gtaataattc catatctttt gacaatattc agaatttgtt caatctcaaa atccccatac
     7621 tctaaattcg gaaaatcttt taacactagt tctactagtt gtattgcttg ctcttcagtc
     7681 atcataaccg aaactagata atttggcttt tctgtctcca cctttatggt agaaaaaacc
     7741 atatcatagt cactactagc tttcacctgt aaatcatcaa tctttgaggt tcctataaac
     7801 tcaatttgag gaaataatgc taatagattc tcttttaaca tcaatgaaga actaacacca
     7861 ttaggacaaa tgattgctgc tttataccat ttttgaggca aagtatctgc tttctttaaa
     7921 taacctccga aatggataac aaaatatgct gtttcactat caggtatggg attgtcaata
     7981 gcgtccatca agggcatcaa agaatctttg actaattcaa ataaatcagg ataatattct
     8041 ttaacatgca atacatattc atttgaacta ggtaggccga actttaatct atagtaagcc
     8101 ggtataaggt ggcggcgaag attttctctc aatccttccc tttgtttaaa atgtaacaaa
     8161 gaaatatctt ccattctact tataatagcc tctgttaatt gattaaagta aaccggagca
     8221 acatctactt caccttcaaa gcaacttgat aataaaactg tgatatagcg ataatcatcc
     8281 tcagaaaaca ccgtatctat aattcccaaa tcaaccactg tatccaataa aatagtggtt
     8341 atatcttgaa taacaggaga tactaatgtc tctgaaagac atactctttc aacatccctt
     8401 tgatacctac acagaatgaa tactaaaccg aaaaggcaaa cttttaattg attaacaata
     8461 ggtactagtt gtagcttctc ataataatct ttaactacct gatttatcaa atcataagtt
     8521 aatgaatacc cccaactgga taaaacataa tccaaacccc aaatccctat ggaggattcc
     8581 agcaactcac taaccatttg aaaagctaag cggtgcttat tccactctga accgtgtaaa
     8641 gtataacctt ttgctctact gtaccctagc tccaaatcat tatctaacat aatctttctt
     8701 aatgattgaa tatcagataa ggttgtattc ttacttactt tcaaaaagtc ttggtaatga
     8761 ttattcgata taaaatctaa tcggcaaaaa gtgtaaagat agattaaagc taagcgagtc
     8821 gactttggta aaaccaattc atccgactta ataatatctg tcaaagactg cttcgtacga
     8881 tttgataaac tatagcgacc ttgtttttta tccagcacta tccctttatt agctagataa
     8941 ggcactaaat aatctattcc ttctttgact tcctttatag gtaagctcac cttaacagat
     9001 aattcatata atgatagctc acaatgatcc atcaaagtca tcaaaataac tagtgctcta
     9061 taatcaaaca tttctcttcc tttctaacta caattatagt gtaaaaatta taagaataaa
     9121 agagtatttg agcacaatat aatctcacct tttaaaagaa attattgggc tgaaagtttt
     9181 tagtgattat tttcaacttt ctattgactt ttaaaaacaa agtgatatca tttagatatc
     9241 aaataatggg aggtcattct tatgactgaa aaactaatca attcaaaacc aaatggtgta
     9301 ttcgcattga ttctcattga gttgacaatc gtacttggtg tctttatatt tataatgggt
     9361 gttggttcgg aaaacatttt tggaattatt atcggacctt tactaatcgt aattgcaggt
     9421 ctagctcatg ctggtttaaa agttgtcaaa cctcaggaag ctctggttct gacactcttt
     9481 ggtaactata caggtaccat caaagaacct ggtttttact ttgtcaatcc cttcagcgta
     9541 gcagtcaacc ctgcaaacca cactcgactt ggacaaagtg gtgatgttag cacaaaatct
     9601 ccttttttag gagctaaatc atcaaatgac aatgatgtaa atcttgaaat tggcaagaaa
     9661 cagatttccc tcaaagtcat gaccttgagc aattctcgtc aaaaaatcaa tgattgctta
     9721 ggaaaccctg tagaaatcgg tatcgcggta acttggagag ttgtcgatac cgctaaggca
     9781 gtcttcaacg ttgataacta caaagaatat ctttcattgc aatgtgatag cgccctccgt
     9841 aatattgtcc gcatctatcc ttacgatgtg tctcctaatg tggatactac gggtgacggg
     9901 caagcagatg aaggtagtct ccgtggctct agcgaaattg ttgctaaccg tattcgtgaa
     9961 gaaatccaaa gtcgtgttga ggatgctggc ttggaaatcc ttgaagcacg tatcacttac
    10021 ctagcttatg ctccagaaat tgctgccgtt atgcttcaac gccaacaagc atctgccatt
    10081 attgatgcac ggaagatgat tgtagatggt gctgtaggaa tggttgaaat ggcactagaa
    10141 cggctcaatg aaggggaatt ggtagaactt gacgaagaac gaaaagctgc catggtttca
    10201 aatctccttg tcgttctttg tggcaatcat gatgcacaac caattgtcaa cacaggaagt
    10261 ctttactaaa gatggctgaa gctaaaaaaa agcagatccc ccttcgactc tcaacaaagt
    10321 tatacgctgc actcgcatca tgggcagaag acgactttcg ttcagtcaat gggcaaatcg
    10381 aatatctcct cacagagtgt gtcaagcaac ggaagaaaga cggaaaatac gtatcagaaa
    10441 ccattgacga accatttgag attgatattt aacatcttct cctgtctgac aagtcggaca
    10501 ggagaatttt ttcatctttt tttgtcaagt aactttactt tacaaaaaaa atgtgttatc
    10561 ctagtatggt tgatgaaaat cagtagattg aatcgaatat aaatacctaa aggagaaatc
    10621 aaaatggcag tacctgcacg tcgcacttca aaagcgaaga aaaacaaacg tcgtacacac
    10681 tacaaagtaa cagctccatc tgtaaacttt gacgaaacta ctggagatta ctcacgttct
    10741 caccgtgtat cacttaaagg atactacaaa ggacgtaaaa tcgctaaagc tgcatcagct
    10801 gaataataga agggagatac catgcgcgta aatattacac ttgaacacaa agaatctggt
    10861 gaacgcttgt accttacttc taaaaacaaa cgtaacactc cagaccgcct tcaattgaag
    10921 aaatactcac caaaacttcg caaacacgtt gtgtttacag aagttaagta agcattcaat
    10981 acgaatcaat acagaagaaa aacgcttgat cagcgttttt tctttttgtt aatttcacta
    11041 tattgcaagg gaaatcattc gaatgactac atttttcact accttttttg aaataagaat
    11101 acggattcgg acgattaaaa gtaagagaaa ctcaaatgta agttggggtt ccttttttgt
    11161 atatttttca aataacgcct ctacactatt tgtagcaaat tcaccaacta cagttgtatc
    11221 ttagttaaaa taagttagaa tatgtaagtg agtaccagat ataccaagac atcgtcacca
    11281 tctaaggtat attcaaaata caaaagttga ccaactagat ttctgaatat ccttatatat
    11341 ccattcttaa aattggttta aatagcgtag tcttttaaac tagttttgag aatccaaaaa
    11401 atcttcctac atatgtaaga agatttttta gttcagaatg attagattta gctaatggat
    11461 acctatccta cccactcacc gttagcattg actcgatagc catcgggtgt agtcgtgttt
    11521 actaataaat ctcctgaatt ataggtatag taccatttac cagaaactgt aa
//
LOCUS       DAVKFH010000061        11243 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKFH010000061 DAVKFH010000000
VERSION     DAVKFH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.061"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            41..379
                     /locus_tag="VW006_001717"
     CDS             41..379
                     /locus_tag="VW006_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380532.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            445..2427
                     /locus_tag="VW006_001718"
     CDS             445..2427
                     /locus_tag="VW006_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380533.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYVQLFITLKQNEYVKKIILGLSKTYIAISSLKYFMMTITMVILFTFLMTGQIE
                     LLYIGAASLLVLMLSIIMSFRKLSESYTKILKGDES"
     gene            2424..3047
                     /locus_tag="VW006_001719"
     CDS             2424..3047
                     /locus_tag="VW006_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9380534.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            3044..3544
                     /locus_tag="VW006_001720"
     CDS             3044..3544
                     /locus_tag="VW006_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380535.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
     gene            complement(3710..5056)
                     /gene="gor"
                     /locus_tag="VW006_001721"
     CDS             complement(3710..5056)
                     /gene="gor"
                     /locus_tag="VW006_001721"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9380536.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            5321..6520
                     /locus_tag="VW006_001722"
     CDS             5321..6520
                     /locus_tag="VW006_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9380537.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            6504..7205
                     /locus_tag="VW006_001723"
     CDS             6504..7205
                     /locus_tag="VW006_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380538.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            7207..8466
                     /locus_tag="VW006_001724"
     CDS             7207..8466
                     /locus_tag="VW006_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380539.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            8590..10587
                     /gene="metG"
                     /locus_tag="VW006_001725"
     CDS             8590..10587
                     /gene="metG"
                     /locus_tag="VW006_001725"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9380540.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            10631..10711
                     /locus_tag="VW006_001726"
                     /pseudo
     CDS             10631..10711
                     /locus_tag="VW006_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            10918..11238
                     /locus_tag="VW006_001727"
     CDS             10918..11238
                     /locus_tag="VW006_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9380541.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         3611 a         1880 c         2355 g         3397 t
ORIGIN      
        1 tttaatcttc atcaaatatt taaattcaag gagaagtaaa atgaaaaaaa gaattaataa
       61 gaaagcatct gtttttctag ttgcaagtgt tttcagtttt agctttgcta ctacagctag
      121 tgtcgttaac gcagtaacgg cacaccctgc tccaagtccc attattgatg tatgggaata
      181 tggtgtaaat gataactatg gttattcgaa ttactttgtt agttctccaa gtaatattgg
      241 atcaaaatct tcagtaacga atttttgggg aacagttaaa gctcaggata aaaaagatta
      301 tggatgggca tattcaagtg caacaaaatc ttggaacgat gttcgcttaa atgcatattg
      361 ggattattat agattttaaa aataatttat gaaaacaaga gacccaatta taagattggg
      421 tctcttgtaa tatggtaatt gcttatgaaa ttaaaattat gtatcattgg attcttcttt
      481 tgtttgattg ctacaattgg tctggtcact attagtgata ccgaaatccc cataccttta
      541 ccaattgatg gagctttttc tatccaagga aaaagtaatc tttctaataa cgaaatatac
      601 gaaatggttc gtgacttatc aaaaacggaa aaggttacca tttacaagcc aatcgttcag
      661 agttcgggtc agttgaagta tgtaaatttt gatgatgtaa ataatgaaca attaaagtca
      721 gcaccaatag tagggatgta ttacacactt gggaaaatgg atgtagacag tttgaaacca
      781 cttacgatga cgggactaca aacagtctat atggcttatc cttggtatat aggaggaata
      841 ttacaattca ctggaacttt aagaatactg ttaatgggtt caatttattt aactttatta
      901 gttgttttat ttgttgttag gacgaggcag atcaaagaag gagtgatacg acgttctttg
      961 ggcttgccta tatacgactt aagaagagag tatggcattt ctcttatttt tgaactgatt
     1021 atgatggctt tattgatgat ttcttacagt tcttttttgg gtaatggttt cttcacctat
     1081 agttctaagt tatttttctc tctgctttta acgaatttta tactatttca aatcattgat
     1141 cttattactt ttgttttatt ttggttgacg attcagattg aaaagcctat tgaaattatt
     1201 aaaaacaagg caaaaaacaa gcttattttt gtcgtatggc ttgctattat ttcgattatc
     1261 atccttgtct caggagtttt tttacaagaa acaaagagta gtcaatctag tattaacata
     1321 cagatacaga atttagtacc atgggataca gtaaaagact ggagaaggat tgagttcctt
     1381 ggaattgaaa gtaactctac taaaaataga gaagttaatg attcagatgg tcagtatcta
     1441 cagatagttg ctgccttaaa aaacttagat ttcttatata tagaacggtc ttcagcatat
     1501 gttccagatt tcatgaaaac ttcacatgtt atagaaaatt tttctaaaca attagaaaat
     1561 gacgggataa cgaatccaga aataaataaa gagttaattt atatcaacca aacaggtgct
     1621 aacctacaaa ataaagtgaa tggaacaaac tatcatcttt tagataataa gatagcaacc
     1681 atctatattc ctgagaagtg gaaagaaaac cagaaatcta ttgagaatac agttgtagca
     1741 gaacaattta ttggaacaaa ctatacaaga gaacagcttg cagttcaaat aattcctgat
     1801 ggggaaaaaa ttttttattt taatgaggat gctgataata atcttaaaat gaaagatatt
     1861 ttaccgctgg caaatgtagc agatagcaaa gataatatag ttgttgtgtt agatacggac
     1921 aaaatgatgg aaaataataa gttttctttg gctagtaaca tactatataa atctcttttt
     1981 agtcctgaag cggtaaaaaa aataaatgag atgactgtcc tgttgaattt ctcaatgaat
     2041 ccagtagatg tttaccaaat tgtgaaatta aagattcaat ccttagagca ccaaatttta
     2101 ctttcacaaa tcttgcagaa gataatttac agtattgtct ttatactcat ttatcaatat
     2161 gtccaacttt ttattactct aaaacagaat gaatatgtga aaaaaatcat tttgggtctg
     2221 tcaaaaacat atatagcaat ttcaagtctc aaatatttta tgatgactat tacaatggtt
     2281 attctattta catttcttat gacagggcaa atagagttgc tatatattgg agcggcttct
     2341 ctgctagttt tgatgttgtc aatcataatg agttttagaa aattatctga aagctacact
     2401 aaaattttaa aaggagatga atcatgacaa ttgacctttt gaacgtttca aaaagtttcg
     2461 gctcaaagaa gatctttaca gacttaaatt taatatttga atctggaaaa agctatgcat
     2521 tgattggagg ttctggctcc ggtaaaagta cccttcttaa tattatagga agattagaaa
     2581 aaattgacag tgggaatgtt ttagttgata aacaagatat ttggaagata aaagaaagaa
     2641 ctttttttaa aaatactgtt ggatatgtat ttcagaatta ttctttaata gataacaaaa
     2701 cagtgtatga taatctgagc cttatcacta aagacaaaaa aacaataact gacgtacttg
     2761 aaaaagtagg actgtcaagt gactatttac accaaaaaat atatgaattg tctggagggc
     2821 aggctcaacg tgtagctatt gccagaatgt taatgaaacc acgtaaaatt atcttagcag
     2881 atgaacctac aggagcttta gatggtgaga ttggaaaaga aattattcgt ttactattga
     2941 atgaaacagc tgaagataaa tatgtcatta tagcgacaca tgatccagct gtctataacg
     3001 aagttgatgt gataattgac atgaaagata tagggtataa ggtatgaaaa agaaaattta
     3061 tattgctttg atctttgtaa ctggggtact cgccattttc ttttttggaa aacaaatgat
     3121 tacaaaagaa aatatcaata agccaacggt agagttaaca atctatacgc tttcttctag
     3181 tgatacagaa aaatggaata aagtgagaca agtagaaacg gaagaagcta tatacttcat
     3241 aactgtgaaa gaagtatcat cttcagaaga agtattttca aacatcattg caaatggagc
     3301 tgctacgggg ttcggagttc gtgaagaaga agtgaagaaa ttcaataatg gtttaggaga
     3361 caccatagag gactctaagc ataataaact aattgaaata gaatttttta ctttttctga
     3421 tgatggcgcg ggatttgtgg tagcaaactt tgactacggt aaagaagagt tgaattctca
     3481 gaaaaaagat ataaaagaat tatataaaaa aatatatgaa tcttttaaag aaaagaataa
     3541 atgattgtat tataatcctt ttattttgct atggtttgtt aattgatttt tgcatatctt
     3601 acttcaacat actataaaat aaaacataag aaaacgagta tctccaacta cggaaatact
     3661 cgctttttga tatttagaag tgttttttgc tttgcttctt ttgtttcgat taacgcatgg
     3721 ttacaaattc ttcagatgca gttgggtgaa ttgccacagt tgcatcaaag tcagccttgg
     3781 ttgctcccat tttgatagca acggcaaatc cttgaatcat ttcatcaacg ccgtagccaa
     3841 ttccatgaag tccgacaact ttttcttctg aaccagctgt tatgagtttg aaacgggatt
     3901 cttgacggtt gcgagtgcaa gcagagtaca tagatgcaaa gcttgattta taaaccttga
     3961 tttggtcttg accgtattct ttaatagctt gctcttctgt caaaccaaca gttccgatag
     4021 cagggtgtga aaagacaaca gttggaatag ttgagtaatc catttttgca gtagtttttc
     4081 cgttaaagag acgttcagat agggtacgtc cggccttgat tgcaactgga gtcagttctt
     4141 tctcgcccgt tacatcacct agagcataga ttccctcaac aacagtattt tggtattcat
     4201 ccacttggat aaagccacgt tcgttcagag tcactccagc tttttcaagt tgcaagccct
     4261 taacgtttgg acggcgacct gtagcccaga taacttggct agctgtgtga ctagtaccat
     4321 cttcgaaatg aatggtaatg ccgtcagtag ttttttctaa cttgacaggg actttgtgag
     4381 tgtgaagtgg taagtttgtt ctttccattt ccttgaccaa accttcaacg atgtaggaat
     4441 caaaaccacg taaaggacga tcgcggcgaa caaagagatc tgtcttgaca ccaaaagtgt
     4501 ggagtacgcc agctaattca acggcgatat aaccagcgcc tagaatggca attgactctg
     4561 gaagttcttc ccaggcaaat acatcatcag aagagccacc tagctcagca ccaggaatat
     4621 taggaatact tggatgggca cctgtagcaa tcacgatatg tttagcacga atcagttcac
     4681 catttacgct tacagtatga gaatctacaa attcagcatg accttcaatc aagtctacac
     4741 cgttgcgttt aaaactacca tcataagaag aacgagcgcg atcaatgtag gcttcacgat
     4801 tgcgacgtag ggttgcaaag tcaaagttaa gatcagtagt cttaaagccg tagtcttctc
     4861 caaattgatg gaaagtctca gcgatttgcg ccccgtacca catgattttt ttaggaacac
     4921 aaccgacgtt gacacaggtt ccacctaatt tcttttcctc aataacggct gctttggctc
     4981 catgttcacc agcacggttc atggtagcga ttcctccgct acctccaccg atagcaatga
     5041 tatcatattc tctcattgaa aactcctttg taatctttta aatagtagag cgtctttttt
     5101 gatagttata ttttattctt tttttgattt gattgcaaaa atctgctacg cacaaaaaat
     5161 tgcaaaaaag cttgccaaat aagtcaagat agtgtattat atatctagta caatagaaaa
     5221 gcaaaattcc gaacgattgt ttaatcctga actgtattgt ttgctggagg gaaatttggt
     5281 ataatagcaa agtattgtaa aaaagaaaag gagtttcatt atgaagaaaa agaatggtaa
     5341 agctaaaaag tggcaactgt atgcagcaat cggtgctgcg agtgtagttg tattgggtgc
     5401 tggggggatt ttactcttta gacaaccttc tcagactgct ctaaaagatg agcctactca
     5461 tcttgttgtt gccaaggaag gaagcgtggc ctcctctgtt ttattgtcag ggacagtaac
     5521 agcaaaaaat gaacaatatg tttattttga tgctagtaag ggtgatttag atgaaatcct
     5581 tgtttctgtg ggcgataagg tcagcgaagg gcaggcttta gtcaagtaca gtagttcaga
     5641 agcgcaggcg gcctatgatt cagctagtcg agcagtagct aaggcagatc gtcatatcaa
     5701 tgaactcaat caagcacgaa atgaagccgc ttcagctccg gctccacagt taccagcgcc
     5761 agtaggagga gaagatgcaa cggtgcaaag cccaactcca gtggctggaa attctgttgc
     5821 ttctattgac gctcaattgg gtgatgcccg tgatgcgcgt gcagatgctg cagcgcaatt
     5881 aagcaaggct caaagtcaat tggatgcaac aactgttctc agtaccctag agggaactgt
     5941 agtcgaagtc aatagcaatg tttctaaatc tccaacaggg gcgagccaag ttatggttca
     6001 tattgtcagc aatgaaaatt tacaagtcaa gggagaattg tctgagtaca atctagccaa
     6061 cctttctgta ggtcaagaag taagctttac ttctaaagtg tatcctgata aaaaatggac
     6121 tgggaaatta agctatattt ctgactatcc taaaaacaat ggtgaagcag ctagtccagc
     6181 agccgggaat aatacaggtt ctaaataccc ttatactatt gatgtgacag gcgaggttgg
     6241 tgatttgaaa cagggttttt ctgtcaacat tgaggttaaa agcaaaacta aggctattct
     6301 tgttcctgtt agcagtctag taatggatga tagtaaaaat tatgtctgga ttgtggatga
     6361 acaacaaaag gctaaaaaag ttgaggtttc attgggaaat gctgacgcag aaaatcaaga
     6421 aatcacttct ggtttaacga atggtgctaa ggtcatcagt aatccaacat cttccttgga
     6481 agaaggaaaa gaggtgaagg ctgatgaagc aactaattag tctaaaaaat atcttcagaa
     6541 gctaccgtaa tggtgaccaa gaactgcagg ttctcaaaaa tatcaatcta gaagtgaatg
     6601 agggtgaatt tgtagccatc atgggaccat ctgggtctgg taagtccact ctgatgaata
     6661 cgattggcat gttggataca ccaaccagtg gagaatatta tcttgaaggt caagaagtgg
     6721 ctgggcttgg tgaaaaacaa ctggctaagg tccgtaacca acaaatcggt tttgtctttc
     6781 agcagttctt tcttctatcg aagctcaatg ctctgcaaaa tgtagaattg cccttgattt
     6841 acgcaggagt ttcgtcttca aaacgtcgca agttggctga ggaatattta gacaaggttg
     6901 aattgacaga acgtagtcac catttacctt cagaattatc tggtggtcaa aagcaacgtg
     6961 tagccattgc gcgtgccttg gtaaacaatc cttctattat cctagcggat gaaccgacag
     7021 gagccttgga taccaaaaca ggtaaccaaa ttatgcaatt attggttgat ttgaataaag
     7081 aaggaaaaac cattatcatg gtaacgcatg agcctgagat tgctgcctat gctaaacgtc
     7141 agattgtcat tcgggatggg gtcatttcgt ctgacagtgc tcagttagga aaggaggaaa
     7201 actaagatgc agaatctgaa atttgccttt tcatctatca tggctcacaa gatgcgttct
     7261 ttgcttacta tgattgggat tattatcggt gtttcatcag ttgttgtgat tatggctttg
     7321 ggtgattccc tatctcgtca agtcaataaa gatatgacta aatctcagaa aaatattagc
     7381 gtctttttct ctcctaaaaa aagtaaagat ggttctttta ctcagaaaca atcatctttt
     7441 acggtttctg gaaaggaaga ggaaattcct gttgaaccgc caaaaccgca agaatcctgg
     7501 gtccaagagg cagctaaact gaagggagtg gatagttact atgtaaccaa ttcaacgaat
     7561 gccatcttga cctatcaaga taaaaaggtt gagaatgcta atttgacagg tggaaacaga
     7621 acttacatgg acgctgttaa gaatgaaatt attgcaggtc gtagtctgag agagcaagat
     7681 ttcaaagagt ttgcaagtgt tattttgcta gatgaggaat tgtccattag tttatttgaa
     7741 tctcctcaag aggctattaa caaggttgta gaagtcaatg gatttagtta ccgggtcatt
     7801 ggggtttata ctagtccgga ggctaaaagg tcaaaaatat atgggtttgg tggcttgcct
     7861 attactacca atatctccct tgctgcgaat tttaatatag atgaaatagc tagtattgtc
     7921 tttcgagtga atgataccag tttaacccca actctgggtc cagaactggc acgaaaaatg
     7981 acagagcttg caggcttaca acagggagaa taccaggtgg cagatgagtc cgttgtattt
     8041 gcagaaattc aacaatcgtt tagttttatg acgacgatta ttagttccat cgcagggatt
     8101 tctctctttg ttggaggaac tggtgtcatg aacatcatgc tggtttcggt gacagagcgc
     8161 actcgtgaga ttggtcttcg taaggctttg ggcgcaacac gtgccaatat tttaattcag
     8221 tttttgattg aatccatgat tttgaccttg ttaggtggct taattggttt gacaattgca
     8281 agtggtttaa ctgccttagc aggtttgtta ctgcaaggtt taatagaagg tatagaagtt
     8341 ggagtatcaa tcccagttgc cctatttagt cttgcagttt cggctagtgt tggtatgatt
     8401 tttggagtct tgccagccaa caaggcatcg aaacttgatc caattgaagc ccttcgttat
     8461 gaatgagatc aacaagatgg acactcgtct atcttgtttt tgtatggatt tccctatcga
     8521 aattcattaa aaatgtgata taatgaagtg ttagaaaaac aggtttcatt tgcctggaaa
     8581 ggaaaaatta tgtctgaaaa aaatttttat attacaacgc cgatttacta tccatctggg
     8641 aaacttcata tcggttctgc ctacacaact atcgcatgtg atgtcctagc acgttacaaa
     8701 cgcctgatgg gctacgatgt attttatctg acaggtcttg atgaacatgg tcagaaaatc
     8761 cagcagaaag cggaagaagc tggtattaca cctcaagcct atgttgatgg aatggcggtt
     8821 ggagttaaag aactctggca attactagat atctcatacg ataaatttat ccgtacaact
     8881 gatgactacc atgaaaaagt tgtcgcacaa gtctttgaac gcttacttgc tcaagatgat
     8941 atctacttgg gtgaatactc tggttggtat tcagtatcag acgaagaatt ctttacagaa
     9001 agccagctgg cagaagtttt ccgtgatgaa gctggaaatg tgactggtgg tattgctcca
     9061 tcaggtcatg aggttgaatg ggtttctgaa gaatcatact tccttcgcct tagcaaatac
     9121 caagaccgtt tggtcgaatt tttcaaagct catcctgaat ttatcacgcc agatggtcgc
     9181 cttaatgaaa tgctacgcaa cttcatcgag ccaggtttgg aagatttggc ggtatctcgt
     9241 acaaccttta catggggagt gcctgtccca tcaaatccaa aacacgttgt ctacgtttgg
     9301 attgatgccc ttcttaacta tgcgacagct cttggctacg ctcaagacga acatggtaac
     9361 tttgacaagt tctggaatgg aacagtcttc catatggtag gaaaagatat ccttcgcttc
     9421 cactctatct actggccaat ccttcttatg atgttggatg ttaaattacc tgatcgtttg
     9481 attgcccatg gttggtttgt catgaaagac ggaaaaatgt ctaagtcaaa agggaatgtc
     9541 gtttaccctg aaatgttggt agagcgttat ggactagatc cacttcgtta ctacctcatg
     9601 cgtaaccttc cagttggttc agacggaacc tttactcctg aagactatgt cggccgtatc
     9661 aactatgaat tggctaatga ccttgggaac ctccttaacc gtacggtttc catgattaat
     9721 aagtactttg atggacaaat ccctgcctat gtagaaggtg tgactgaatt tgatcatgtt
     9781 cttgctgagg ttgcagaaca atcaatcgca gacttccata cacacatgga agcagttgac
     9841 tatccacgtg cgcttgaagc agtctggact ctgatctctc gtaccaataa atacatcgat
     9901 gagactgcac catgggtctt ggccaaggat gaagctcttc gtgaccaatt ggcaagtgtc
     9961 atgagccact tggcagccag cattcgtgta gttgctcact tgattgaacc atttatgatg
    10021 gaaactagtc gtgcagtttt gactcaactt ggtttggaag aagtttctag tcttgaaaac
    10081 ttgagtttgg ctgacttccc agcagatgtg actgtagttg ccaaaggaac acctatcttt
    10141 ccacgtctaa atatggaaga agaaatcgcc tatatcaagg aacaaatgga aggcaataaa
    10201 ccagcagtcg aaaaagaatg gaatccggac gaagttgagc tcaaactaaa caaggatgaa
    10261 atcaagtttg aagactttga caaggttgaa atccgtgtcg cagaagtcaa agaagtgtct
    10321 aaagtagaag gttcagataa gttgcttcaa ttccgcttgg atgctggtga tggagaagat
    10381 cgtcagattc tttcaggaat tgcaaaatac tatccaaatg aacaagaatt ggtcggcaag
    10441 aaagttcaaa tcattgctaa cctcaaacca cgtaaaatga tgaaaaaata tgtcagccag
    10501 ggtatgattc tctcagctga acatgatggc aaattaaccc ttctcacagt tgatccagct
    10561 gtaccaaatg gaagtgtgat tgggtaaaag tagacaggac tagttcaggc tagttctgtt
    10621 tttttattta tactcaatga aaatcaaaga gcaaactagg aagctagccg caggttgctc
    10681 aaaacacagt tttgaggttg tagatgaaac tgacgaagtc agctcaaaac acagttttga
    10741 ggttgtagat gaaactgacg aagtcagtaa ccatacataa ggtagggcga cgctgacgtg
    10801 gtttgaagag attttcgaag agtattaact attatgcttt acaaactagt gtagaagtga
    10861 tatactatag gtgaagttac tagtaggtat tacaaaatag attagaaggg aaaagtgatg
    10921 aaagaaactc agctattaaa aggtgttctt gaaggttgtg tcttggatat gattggtcaa
    10981 aaagagcggt atggttatga gttggttcag actttgcgag aggctggatt tgatactatc
    11041 gttccaggaa ctatttatcc tttgttgcaa aagttagaaa aaaatcaatg gataagaggc
    11101 gacatgcgcc cgtcgccaga tggtccagat cggaagtatt tttcattaat gaaagaagga
    11161 gaagagcgtg tctcagtctt ttggcaacaa tgggacgatt tgagtcaaaa agtagaaggg
    11221 attaagaatg ggggttaaac cat
//
LOCUS       DAVKFH010000062        11174 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKFH010000062 DAVKFH010000000
VERSION     DAVKFH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11174
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.062"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            27..1022
                     /locus_tag="VW006_001728"
                     /pseudo
     CDS             27..1022
                     /locus_tag="VW006_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1048..1692
                     /locus_tag="VW006_001729"
     CDS             1048..1692
                     /locus_tag="VW006_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9380542.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1734..3722)
                     /locus_tag="VW006_001730"
     CDS             complement(1734..3722)
                     /locus_tag="VW006_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380543.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3724..4482)
                     /locus_tag="VW006_001731"
     CDS             complement(3724..4482)
                     /locus_tag="VW006_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380544.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4910..5101
                     /locus_tag="VW006_001732"
     CDS             4910..5101
                     /locus_tag="VW006_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380545.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5455..5727)
                     /locus_tag="VW006_001733"
     CDS             complement(5455..5727)
                     /locus_tag="VW006_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9380546.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5786..6235)
                     /locus_tag="VW006_001734"
     CDS             complement(5786..6235)
                     /locus_tag="VW006_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9380547.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            complement(6222..8351)
                     /locus_tag="VW006_001735"
     CDS             complement(6222..8351)
                     /locus_tag="VW006_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9380548.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            8395..8478
                     /locus_tag="VW006_001736"
                     /pseudo
     CDS             8395..8478
                     /locus_tag="VW006_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            8467..8607
                     /locus_tag="VW006_001737"
     CDS             8467..8607
                     /locus_tag="VW006_001737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380549.1"
                     /translation="MDKTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNRTTVR
                     RS"
     gene            8853..9758
                     /locus_tag="VW006_001738"
     CDS             8853..9758
                     /locus_tag="VW006_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9380550.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            9755..10570
                     /locus_tag="VW006_001739"
     CDS             9755..10570
                     /locus_tag="VW006_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9380551.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(10700..>11174)
                     /locus_tag="VW006_001740"
     CDS             complement(10700..>11174)
                     /locus_tag="VW006_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9380552.1"
                     /translation="LILLDGGYLDLDKILPLDTELEETKNYIKSQIISDLDVLTSKEK
                     SEAKHWSENMEKAVRQSYHWNVEYNRYELAINYENIEAILRLRRKIQAFKREVGDTLF
                     ISPRYPNEATWREEALKELPDYFDTIFLENFGHELYTQAPKEIASLMNEWLAYFL"
BASE COUNT         3352 a         2342 c         2155 g         3325 t
ORIGIN      
        1 accacaaaca caagaaaggc acagagatgc aagacaatta tactacaaaa gccaaacatt
       61 tgacaatcga tagccgtcgc ttaatcgaaa gatggaaaaa agaaggaaaa tcaaatagag
      121 aaattgcctc tctacttgga aaagctcctc aaactatcca cactgaaatc aagcgtagga
      181 cagtccgaca atgtcttgga aaagggcgct tcaaagaggt ttattctgcc gactatgctc
      241 aacagtctta tgaaaataat cgcaagcact cggtcaagag atcaagcgtg accaaggaac
      301 taaaggaaaa gattctccac tatcataacc aaaaattttc gcctgaaatg atggttatgg
      361 ctaaaggggt taacgtggga atttcaacca tttactattg gattcatcat ggaaaattgg
      421 ggttaagcaa gcaggatctg ctttacccta gaaaaggaaa agcgcttaag aaacaggcta
      481 gcaccaactt taaacctgct ggtcaatcca tcgaacagcg gcctgaagct atcaatcttc
      541 gcttggagaa tgggcattat gagattgata cggttctact tacgagatcg aaaaactact
      601 gcttgcttgt cttgacggat cgaaagagta gacatcagat catccgattg attccaaata
      661 aaagtgctga ggtggtcaat caggctctaa aactcatctt aaaacaacac aagattcttt
      721 ccatcacggc agataatgga acggaattca atcgcttgtt tgatatattt tctgaggagc
      781 acatctatta tgcgcacccc tatgcctctt gggaaagggg aactaatgag aatcacaaca
      841 ggctcattcg tagatagtta cctaagggaa ccaagaaaat gactcccaaa gaagtcgcat
      901 tcatcgaaaa gtggattaac aactatccta aaaaatgctt ggactacaag tcacccagag
      961 aagacttctg gatggctaac ttgaacttga aatttagcgt cagaaacaaa tcacgaaatt
     1021 gattcaaatt ttgcaggtcg tttaaatatc ctgcgtgcgg gtgttcttga tgctaacgat
     1081 ggaattattt ccattgctgg tgtggttatc ggagttgcca gtgccacgac caatatctgg
     1141 attatctttt tatcaggttt tacggctatc ttagctggtg ccttttcaat ggctggtgga
     1201 gaatatgtat ccgtttcaac tccaaaagat accgaggaag ctgccgtttc gcgagaaaaa
     1261 ctcttgctag accaagatag ggaactagcc aaaaaatccc tctatgctgc ttatatccaa
     1321 aatggagaat tcaaaacttc tgcccaactc ttgaccaata agatctttct taaaaatcca
     1381 ctcaaggctc tggtagagga aaaatatggg attgagtatg aagaatttac caatccttgg
     1441 cacgctgcca tttctagctt cgttgccttt ttccttagaa gtttgcctcc aatgctgtca
     1501 gtgaccattt ttccaagtga ttaccgcatc cctgctaccg tccttattgt cggtgtggcc
     1561 cttcttctca ctggttacac aagtgctaga cttggaaaag ccccaaccaa aacagctatg
     1621 attcggaacc ttgctattgg tctcttgacc atgggagtta ccttcctgct cgaacaactt
     1681 ttcagcattt agaatacaag aaatacctcg attttgaagt cgaggtatct tttttacatt
     1741 tgcacaatct tgcgataact tcttgaagta atcatgaaaa tcagcacata ggcgatgagg
     1801 aagatagcgc agatagacaa ggtcacaatc aacatcatag tcgtatccag tacaccaatc
     1861 acttttaaaa tcaggctaag catatggtag gcaaaggcga gatgtatgaa ggcaaagagc
     1921 aaaggaagga agaaaacagt taaaacctgt ttgttgatgg tttgcttgat ttgcttttgg
     1981 tccaaaccga ctttctgcaa gataataaag cgttcacggt cttcgtagcc ttcagaaatt
     2041 tgtttgtagt agatgaccag aacagttccg accataaaga taatggatag gaaaataccg
     2101 ataaagaaga caccaccaaa gagggcactc atctgagaac tagcatctgc tagattgcta
     2161 ccataaacat agctaccttc tgtgtctaat tgagcattaa attgattgag gtagttttca
     2221 tactcctcag cgaccttgag ttgttcttct tcgctggcat ctacattcat accaccgtaa
     2281 aactgattat agatagccga atctgggaat tgatccaaaa aggcttgtaa atcaggaaca
     2341 acaaggtaat tgtaatcagt agtcaagata ttaaacttat ttggaacatg gttgacaata
     2401 aaatctttag taaattcttc tttgacagaa aattgatgat catttagagt tagagctttc
     2461 tgtcctttca gtccgtcatt tttggcaaag agaccgacct catttcctga tagagacagt
     2521 ttttgaccag tcatattttc ataatctttt tggtcaaata ccatgaaaac tgtttttggt
     2581 tggacacggt tttgtccttt ttcaaaaata gttaacttgg ttccttcttg attcgcaaca
     2641 gcaaagtaag tgtaacgaaa tacttctttt tctttaatct tataaccttt gtcacttgca
     2701 aactggctca agagtttgtc caagtcttct ttttcaacat tttgccctga aaccccaaaa
     2761 tcatgaggat ttagaacttt tttaaagctt tctgaggaat tgaaaatgct tgtcgctgct
     2821 gacatggtta ccaaaaccat tgttgacaag atagcgatgg tggctagtcc aactgcattt
     2881 ttcttcatac ggaaaatcaa gttagaaaca gatatgaggt tatttggttg gtaatagtat
     2941 ttcttatttt tctttaagat ttggaggaaa acggtaatcc ctgcattaaa caagagataa
     3001 gtcccaaaga taaccagtaa aacagctatg aagaaggttg ttaaggctgt aagaggatct
     3061 tttaccgtaa gggcaagata atagccaatt cctaaactta tggaaccaag aatggtttgg
     3121 agaggaagga agcgaccttt tttctctcca ctagctttct cacgagagag ctggagggca
     3181 ttcatacggg cgattcgaag ggcattcagg aacatgaggc ctaggaaaat caaaccgaag
     3241 acaacaagca ctgtaatgac aactttcgtc tggaaggtag caaccagctc aaccttcaat
     3301 ttcattagtt tgagcaggaa agcgaaaatt aacttgtcaa acaaggctcc aataccgata
     3361 cccgctccaa cagttagaat cccaaatacc actaactcct taaaggtcat actgataaga
     3421 tgacgcttct ccaagcccaa catgccataa attcctagtt ccttggaacg gttcttcatg
     3481 acaaaactat tggcatagag aacgataatg gctgacgcaa gggtgacgac aaacatacca
     3541 aatccaagtg tagcctgaat ggttgttcct ccacggattt ccgcaatctt aggattgaag
     3601 gttagagagt aaaagagata ggtgacagtg actgccaaga gaacagccag cgcaaaagga
     3661 tagtagagtt tgcggttttt aatcaagttc gataccgcta acttattggt taatcgaaac
     3721 atactaattc acctcgcttg ccatgacagt caaggtatca gagatttctt ggaacatctg
     3781 acgttctgtc ttctctccac ggtagatttg attgtaaaga atgccgtctt tgataaagag
     3841 tacacgttta gctctgctag ctgctgctgt tgagtgggtt accatgagaa tggtttggcc
     3901 gcgctcattg atttcatcaa agacatcaag taaggctgca gatgacttgg agtcaagagc
     3961 tcctgtcggt tcgtccgcaa ggagaatttc aggttctgtg atgatggcgc gggctactgc
     4021 tacacgctgt ttctgaccac cagaaatctc gtaagggtac ttctcttgta attggtttat
     4081 tcccagattc tcagctgtca ccaccaattt cttcatcatc tctgtaatag gtcttcttga
     4141 caagacaagc ggaagcaaga tattgtcctt tacagataga gtatctagca agttaaagtc
     4201 ttggaagaca aagcccaact tttcacgacg gaagctagag gcctgtgaat ttttaatggt
     4261 tgcggtgtca gttccattca agtaaacctg accacgactt ggtttatcca gcatagctag
     4321 aatattgaga agagttgatt taccagaacc agactcaccc atgatggcaa cgtagtcacc
     4381 cttttcgacg gtaaagtgaa tatccttgag ggcttctact tggttgccct gaaaacgtgt
     4441 tttataaatt ttttgaacgt gttttacatc taaaagtgtc atgagaatct cctttcttaa
     4501 tatcccatca aatgaaatgt agcttgcatc atagtgcatc ccagctgaac gcgctcaatt
     4561 tctttgtgac ttggttttct agttttctta tgtaagattt gtttcatgat gttactcctt
     4621 tgttctttat gagtctagtt tacatcaaaa aaagaccgct tgccataacc taaccttaca
     4681 aaagagactt taatcttaca tttttgtaag attgggtgaa atataagcaa aattacttag
     4741 aaaatgattt gatttcctat ttttaaagtt gtaaaatata agtgtgaaaa ggcttacttt
     4801 gaaaaggagg aagcgggaac taacatttta gtaaggttac ctagataaca taccattcaa
     4861 tttaacactt gcaagtcgtt caacctataa ggaaagaagg tgcgctttaa tgaaacacaa
     4921 agaacatata ctcatcggac ttctctatct gctctctcca ttcatcggtc agctcttagt
     4981 tgaacacaca cactttatcg gtacagaatt taccggcact gcttatgtta tttgctggtt
     5041 atcagttgtt atcagcatcc accatttttc caaaaatgta ttgtctcagc agcaaaaata
     5101 gattaaaaaa ctgagtttaa attactgctt ttataataaa atgaaataag aacaggacaa
     5161 atcgatcagg atagtcaaat cgatttctaa caatgtttta gaagtagagg tgtcctattc
     5221 tagttccaat ccactatagt ttgtggatga agctggcgaa atattattaa tactattcaa
     5281 aaatcaaagt gcaaactatg tcaacttcac attgccgact aaattacagc ctgtggctgg
     5341 cttcctagtt tgcactttga tttttattga gtataaaagt aaaaaagctt taaatcaagc
     5401 agtttagact attgatttaa agctttttgc ttattccaaa tcttctgtag aacctcacca
     5461 aaaccagcct tcattgtcgt caatggcttg ggcttctttg cgtttacgtg ggcctgttcc
     5521 gtacatttct gcttcgattt cttccttggt aggcatgata gaagctagga tgatatagat
     5581 aatcacgcca agtccaaagt ttgcaactgt aaaaatagca aacaagaaac gaactagggt
     5641 aacatcaaaa ttccacttgt ctgacagacc tgccagaact cctgaaatca tgcgattgcg
     5701 tctcatttta taaaatttac tgttcatgat atttcctctt tctgctaggt tagacatagt
     5761 atatcacggg gaggctcggc tttcatcagt cctcaggctg atttatcaga agcaatttac
     5821 ctcgacaaag tccacagcga tagcgtttgg tatcaatcct acgcttgcgc tgataccttt
     5881 gctggcagga ttgacaacga taaagcttga gtggtttaga gttactattg ggcaaggatg
     5941 gtacaaaccg taatccatcc actgctttca aaagttcctt aaaatcccga tccttgtgtt
     6001 gatagccctt cccttggaaa taaaggtgat aatgacagag ttcatgtcgg acaattttcc
     6061 taaaaacctc catacccagt ttctgataaa ccttgggatt aaaatccaga tgcccatctt
     6121 tggggaaaaa tcgcccacct gtcgaacgta gacgcctatt ccactggaca tgatggataa
     6181 aagctctccc gaagtcttct agtgaaacct gcttgacata atcagtcagt ttcatttgga
     6241 gcgaggagcg acagattaac tttttcacgt tcagtatcaa ttttcttgac ccaaacggtc
     6301 accaaatctc caactgccac cacttgacta gggtgtttga taaacttgcg actcatatga
     6361 gaaatatgaa tcaagccgtc ctcgtgaatc ccgatatcaa cgaaggcacc gaagtcaaca
     6421 acgttacgca caacaccttc tagcttctga cctaccacta ggtctttgat atctaggaca
     6481 tcttggcgaa gcacaggtgc gtcaaaggaa tcacggaaat ctcgacctgg tttgagaaga
     6541 tctgcaatga tatctttaag ggtttctgga ccgaggtcta gctcttgagc catttccttg
     6601 actgaaagcg acttgagttt gctttgggcc tcttcattca gatccttaat gtccaagcat
     6661 ttgaagagtt ccttaacggc agcgtaattt tctgggtgaa ctcctgtatt atcaaggata
     6721 ttgctacttt cagggatacg gaggaaacca gcagcctgtt caaaagcctt ggcccccaaa
     6781 cgaggaactt tcttgatttg ggagcgtgaa gtgatttttc cctcttcctc acggtatttg
     6841 acgatatttt cagaaatggt tttattgagt ccagctacgt gtgaaagaag agctgggcta
     6901 gctgtattga cattgacacc aacttggtta accactgtat cgacaacaaa gtccagactc
     6961 tcagatagtt tcttctgact gacatcgtgt tggtattgac cgacaccaat tgacttagga
     7021 tcgattttga ccaattccgc aagaggatct tgcaaacgac gggcgataga aatggcagag
     7081 cgtttttcaa cggtcaagtc tggaaactcc tgacgagcaa gttcgctggc agaatagaca
     7141 gaagcaccac tttcattaac gataacatag ctgacttcag ggaaatcttt cagaacttcc
     7201 gctacaaaag cttcactttc acgactggcc gttccatttc caatggcaat aatctctaca
     7261 ccgtattgac caattaaatc tgctaaatct ttcttggctt cttcgatttg acgagctgat
     7321 gctggtttaa caggataaat aacctgagtt gtcagcattt ttcctgttgc atccacgaca
     7381 gctagcttgg cacctgtacg aaaggctggg tcaaacccaa gaaccacgcg ccctttcagt
     7441 ggagcaacca agaggagatt gcgcagattg tcagaaaaaa gttggatagc tccctcttca
     7501 gctttctcag ttaattctgt ccgaatacga cgctcaatag caggcaagac ctttttctta
     7561 acggattgct gaacaacttc atcaatataa gcatttttca ccttgaaacg agtagcaaag
     7621 aaggcaagaa tacggtccgt cgcatgttca aaaccgatct tcaagacacc aagtttctcc
     7681 ccacgattga gagccaaggt acgatagcct tgcatagttc caactgtctc tgaaaaatca
     7741 taataaatct gaaaaacctg cttttcatca agactttcat ccttggcttg agaagtgagt
     7801 ttagagtgtc tcagcacttc ctgataagtc atagaacgca aggtcacatc ttccgataag
     7861 gcttcgacca aaatatcaac tgcaccggtc aaggcttcct tgccagtcgc aaatccttca
     7921 cagacgaact tttcagcttc tttctctaag tcaactatat tctgcaaaat caaacgagca
     7981 agaggaaaga gtccagcttc acgggcaatg gttgccttgg tacgacgctt ttccttataa
     8041 ggaagataga gttcttcaac gtctgctaat ttttcggcaa ctaagatagc ttcttccaat
     8101 tccttggtca acttaccttg ttcttgaatc ttagctaaga cagcttcctt acggtcattg
     8161 agatttgtca gacttttatc caaatcaata atagccttaa tcgccacctc atccagacta
     8221 ccagtcatgt ccttgcgata acgcgcgata aagggaatag tcgccccttc agctgtcaaa
     8281 cttagaacgg tatcaatttg ctttaacgtc actcccaaat cctgagagat tttttcatat
     8341 tttttatcca taaatctatt ataccacaag ctggaaggct ttctccaatc cttttcatac
     8401 tcaataaaaa tcaaaaagca aactaggaag ctagccgtag gctgctcaaa atactgtttt
     8461 gaggttgtgg ataagactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga
     8521 actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt
     8581 aaccgtacta cggtaaggcg aagctgacgt ggtttgaaga gattttcgaa gagtatcaac
     8641 tatcgaaaac aaattaaaaa actccctttt ccgcaaaata atagtataat agaggtagaa
     8701 tttagaatcg aggtacacct atggctgtca aatttacaaa acgagacgac ttggacaaga
     8761 tgtttgaaga gtttgctaaa ctccctgatt tgaaacaagt tactttccct gatgacaaag
     8821 agaaaaaagt caaagcagaa aagaaaaact agatgactgc ttttcaacaa ctcccatcta
     8881 gtgtacttca aactggagcc atttttctct ccattatcat tgaagccctt cccttcgttc
     8941 tgataggaag cattgtctca gggctgattg aagtttatat cacacctgac aaggtttatc
     9001 attttctccc tcgaaatcgt tgggggagaa tcttttttgg gacctttgtc ggtatacttt
     9061 tcccttcttg tgaatgtgga atcgtcccca tcatcaatcg ttttctggaa aaaaaggttc
     9121 caagttacac ggccgttcct tttcttgtga cagcacctgt tatcaatccc attgttcttt
     9181 ttgcgaccta ttctgccttt ggcaactcct tccatgtcgc cctattacga gctctgggtt
     9241 ccattcttgt ggctgtaata ctaggaattt ttctaggatt tttctggcaa gaaccgattc
     9301 agaaagaaaa tcgtctggct tgtcatgagc atgatttttc ttacttgagt tctgcaaaaa
     9361 aagtttttca agtctttgtg caggccattg atgaattttt tgatacgggg cgttatttgg
     9421 tatttggctg cctctttgct tctataatac aggtctacgt tccgactcgg attctgacct
     9481 ctatcagtgc gacccctctt tttgccatcc tgctcttgat gattttagcc tttcttcttt
     9541 cgctctgtag tgaggcggat gcctttatag gtgcttctct tctctcgagt ttcggtttgg
     9601 caccagttct ggcctttctc gtcattggtc caatgctgga tatcaaaaat attctcatga
     9661 tgaaaaatta cttgaaagca cgatttatca gtcacttcat aacaattgta actcttgtcg
     9721 tcttagtcta ttctctcttg attggagtca tcctatgatt cgatttttag ttttagctgg
     9781 ctattttgaa ctgactattt acctccatct gtcgggcaaa ctaaaccagt acatcaacat
     9841 gcactattcc tatctggcct atatctccat ggtgctttct tttatcttgg ctatcgttca
     9901 attgtatatc tggatgaagc aagtcaaaac ccacagtcat ctgaacagcc gattggccaa
     9961 gatgacgagt atttctcttc tggctattcc acttgtcatc ggcttaactt tcccaactgt
    10021 tagcttggat tctcagactg tttcagctaa aggctatcat ttccctctat cggaaggaac
    10081 gaatctggct attcagacaa gtgaagggac tacaagccaa tatttgaaac cagatactag
    10141 ttcttatttt tcaaaatcag cctatgaaaa ggaaatgcga acggcggcgg ataaatactt
    10201 atcccaagac agcattcaga tcactaatga aaactatatg gaagtcatgg aggctatcta
    10261 cgactatcca gatgagtttg agggcaagac aatccagttt acaggctttg tctataacga
    10321 ccccagtcat gccaatagtc aatttctgtt ccgattcggc attatccact gtatcgcaga
    10381 ttctggtgtc tatggattgc tgaccaaggg caatacccgg cagtatgaaa acaacacttg
    10441 gatttcagcc aaaggaaaac tggtcaatca ctaccataaa gaactcaaac aaaaccttcc
    10501 aaccttggaa atcgacagct ttaccaaagt cgataaacca gaaaatccct atgtatatag
    10561 agctttttaa gaaaatcaag ataaaaacga acaagttctc ttctgaataa cagaaaaaga
    10621 gcctgttcgt tttttggtta tatgaaaatt agtgacttgt agattttcat cttataccat
    10681 ttccagcaat acaagtagct catagaaaat aagcgagcca ctcattcatt agactagcga
    10741 tttctttagg tgcttgagta taaagctcat ggccaaagtt ttctaaaaaa atagtatcaa
    10801 aatagtctgg caattctttt agggcttcct ctctccatgt agcttcatta ggatagcgag
    10861 gactaataaa caaggtatct cccacttctc tcttaaaagc ttgtattttt ctccgtaggc
    10921 ggagtatcgc ttctatattt tcataattta tagccaactc atatctatta tactcaacat
    10981 tccagtgata ggactgtctt acagctttct ccatattttc tgaccaatgc tttgcttcag
    11041 atttttcttt agaagtaaga acatctaagt ccgaaataat ttgagatttg atataatttt
    11101 tagtttcctc taactctgta tccaaaggta aaatcttatc taaatctaga tagccaccat
    11161 ccaaaagaat cagt
//
LOCUS       DAVKFH010000063        10970 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKFH010000063 DAVKFH010000000
VERSION     DAVKFH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.063"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            11..733
                     /locus_tag="VW006_001741"
     CDS             11..733
                     /locus_tag="VW006_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9380553.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VW006_001742"
     CDS             730..1578
                     /locus_tag="VW006_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9380554.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VW006_001743"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VW006_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9380555.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VW006_001744"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VW006_001744"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9380556.1"
                     /translation="MVTFLGNPVSFTGKQLQGGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VW006_001745"
     CDS             complement(3249..5957)
                     /locus_tag="VW006_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9380557.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGIVVGETQIQFYWTYSLLALVLSWLASVLPVYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSETPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VW006_001746"
     CDS             complement(5959..6660)
                     /locus_tag="VW006_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380558.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VW006_001747"
     CDS             complement(6910..9321)
                     /locus_tag="VW006_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9380559.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAVRASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9374..10066)
                     /locus_tag="VW006_001748"
     CDS             complement(9374..10066)
                     /locus_tag="VW006_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9380560.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10117..10407)
                     /locus_tag="VW006_001749"
     CDS             complement(10117..10407)
                     /locus_tag="VW006_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380561.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            complement(10601..10957)
                     /locus_tag="VW006_001750"
     CDS             complement(10601..10957)
                     /locus_tag="VW006_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380562.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGWVGGILVII
                     GGSLFLATLKKFKSEE"
BASE COUNT         3206 a         2524 c         2053 g         3187 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ctgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gcatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcccac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aaggcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtcc tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattagc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttaataaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccgag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggag tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcggcatg aagttccctc caagccacca gatagactgg taacacactc
     4501 gccaaccaac tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ctatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggtgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggatgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttgtcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag ttgcagacgt
     5221 accttgccat tatctgataa agtttgttca aggtcttcct gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ctgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgcctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat ttgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tgtcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 gagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tattgagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagaactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac aattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct gcaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgttt tttcaactag
     6841 ttgaaaactg cttatattct taaatcctta ttaaaaggca gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatgtc tttaactgct ctgccaataa
     7321 tttatgggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggctagca cgtaccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 atgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataagtatt gccaatcgaa atcggcccat gttctgggga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctcc ctgagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttctcctcac ctttctgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgact ggaaagaccg tctctaggag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tgaatgcgtc
     8341 aacattagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgctcatact ttccatcaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttccg ccccttcctt agtaaaacgc tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcac
     8881 aagtgcctta ctaatattca gctgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaac atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagcccct ataaggccaa atacttttgc tcctaaagaa ccattgccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga aactggttgc ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aagcctagaa atcaattcta
     9361 gacttacaat tttttatttt ccaaggtagt attcagaaac gacgttcaat ttttcgtcga
     9421 attcgaatac caatggtggg aagttaggga tttccacgtc catgatctcg tcatctgaca
     9481 aacctttgat gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt
     9541 ttttaccatc tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca
     9601 aagtcacttt caagttttca gcatctggga ttactgagtc gtcaagtgaa gcataacgac
     9661 ggtctgtgtg agctgagtgc tcatcatcac ggtccatatt tggaggcaat acatcgtatg
     9721 aacgacgcca gatgtgaact tgctcatcac caaattgttc agcagcttca gctttatttt
     9781 taccagtcaa accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc
     9841 acaattggtc agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg
     9901 aagtgtaagc ttggtcaaat ttgataccag cttctttgat caatttacca gcgtcaatcg
     9961 cttgttgtgt acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt
    10021 tgttccattc agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc
    10081 tttaaatttt ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa
    10141 aaagctagtc aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt
    10201 tttagttgtt tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt
    10261 ttgagttgac tcttttagtt tagtaaacga gtcattattt tccatagctg tgcctttgaa
    10321 accttcctca gctttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc
    10381 tgcataagtg cgtattgatt caatcacatc gtctgttaca accggtgtat cgaaagtgat
    10441 tgtttctttt gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatc
    10501 ctttgaactt tatgttccac atgcaactag aagaaaaaga gataatagag ctactcctat
    10561 accgataaac ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt
    10621 tcttcaatgt tgcaaggaat agagatcctc cgataataac aagaatcccc ccaacccatc
    10681 ctaagaatgg aatgagacta actccaccaa aaacaatcat tagtacagac ggagctgcac
    10741 ctacacgatt atctccttta tagtaaacaa tagcaatgat accaagaaca agatttgcaa
    10801 tcttcaaggc attcaagagt aaggcaacac ctgaaaaagc ccctgcagta gctcctccat
    10861 taagagttgt agcaactgtg tttaccgctg ttcccaacaa gacaaaagga ccaataagaa
    10921 gtaaaattcc tcccacaaga ccgacgatac cactaattaa agcaagtgtt
//
LOCUS       DAVKFH010000064        10880 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKFH010000064 DAVKFH010000000
VERSION     DAVKFH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.064"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(284..1090)
                     /gene="yidA"
                     /locus_tag="VW006_001751"
     CDS             complement(284..1090)
                     /gene="yidA"
                     /locus_tag="VW006_001751"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9380563.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1103..2407)
                     /locus_tag="VW006_001752"
     CDS             complement(1103..2407)
                     /locus_tag="VW006_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9380564.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2479..2856
                     /locus_tag="VW006_001753"
     CDS             2479..2856
                     /locus_tag="VW006_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9380565.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2947..3279
                     /locus_tag="VW006_001754"
     CDS             2947..3279
                     /locus_tag="VW006_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9380566.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3291..4862
                     /gene="ffh"
                     /locus_tag="VW006_001755"
     CDS             3291..4862
                     /gene="ffh"
                     /locus_tag="VW006_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9380567.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRYITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5057..6340)
                     /locus_tag="VW006_001756"
     CDS             complement(5057..6340)
                     /locus_tag="VW006_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9380568.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6643..7356)
                     /gene="rsmG"
                     /locus_tag="VW006_001757"
     CDS             complement(6643..7356)
                     /gene="rsmG"
                     /locus_tag="VW006_001757"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9380569.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7450..8010
                     /locus_tag="VW006_001758"
     CDS             7450..8010
                     /locus_tag="VW006_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9380570.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            8012..8911
                     /gene="htpX"
                     /locus_tag="VW006_001759"
     CDS             8012..8911
                     /gene="htpX"
                     /locus_tag="VW006_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9380571.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(9060..10619)
                     /locus_tag="VW006_001760"
     CDS             complement(9060..10619)
                     /locus_tag="VW006_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9380572.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
BASE COUNT         3176 a         2018 c         2434 g         3252 t
ORIGIN      
        1 tgaagtatcc taatttgtat aattgattgt gtgtagattc agggtcaatt tgaagagagt
       61 atttaaaata tcttgggaaa ttataatttc tccctgtttt ttgagtccaa tctatgagta
      121 tgagttgccc aggagtaagg ccacattcta aatcgtttag gtaaaaattg ttacaatcag
      181 aatttgtgtc tctattatga ttattttgtt ttaaattaaa tagtttgcta aaaaaattca
      241 ttggatttta ttgctaatca attcttattg aaaaatgata cttttacagt acccatgttc
      301 ggatggcgtg ggcaacgccg gattcgtcat ttgttttagt gatgtatttg gcgatttttt
      361 tgatttctgg atttccattt tccatgacaa cggggtttcc aacgacttcc agcatggcac
      421 ggtcattttc ttcgtcaccg attgccatgg tttcatcttt ggtcaatccg agtttttcag
      481 ccaagtgagt aatagctgaa cccttgtcta cattcttttt aaggagttcg aggtagaaag
      541 gagcagattt gttgatagag tagtgctcgt aaaattcggc tggtattttt tcaatcgcag
      601 catcgagaat ttctggttca tcgatgaaca tacacttgac aatttccttg ccagccattt
      661 cttcaggggt acggtagaag ataggcatgc tgacgagggt tgattcgtgt acagtgtatg
      721 ttccgatatt gcgatttgca gtatagatac cgtccttggt aatggcatgc atgtggacac
      781 cgagcttgcg actgaggaat tccatatcca gataatcctc ataggtcaag gattcgctga
      841 taatctcatg gccagtagca gtttcttgga caagggcacc attgaaggtc accacatagt
      901 caccttggtc tctcaactgc aagtcgtcca gaagtttggc aacacctgcg atggggcggc
      961 cagttgcaat cacgactttg acaccagctt ctttggcatc ttggatggca gaaaagactt
     1021 caggagtgat ttccttttgg ctgttgacta gggttccgtc aatatcgacg gcgattagtt
     1081 taatactcat aaatttcctc ttttagtttt tatttggggt aaaatgatcg ttctcaatca
     1141 agtgtaaaaa ttgctgggtg atgcttgcga agatgctgtt ttggtctaac atttctttcg
     1201 ggaaataaaa gcgattatct ccgtggcgac ttccagcaag ggattggacg ataggggaca
     1261 ggctagagag ttctgccagt tctccatttt tttgtaaaat ctcaatctgt gtccttggat
     1321 tttcagattc gggacggtaa atatcataag ggaggtcaaa gttcttatga atggcagtat
     1381 agtagtcggg atcaaagccg atgtcctcaa ccaattttct catgctagcg agttggtctt
     1441 ggtcttcttg tgaaaaggta atggatttaa agaccttgcg gttgacaaag cgatgcgata
     1501 aatctgcaag aatcttgtca ggactggtca tccaaagctg gaagtaggta ttcatcacgc
     1561 catcatccag agccagatag tcagtcaagg tcacattttt ttcgaagaaa ggcaggaggt
     1621 gtggagaagt tcgggcaaag aaatccttgt cctcaggata gagttccttg gcgcgtttga
     1681 gaagattctg taggagaact tccatggcgc gtgttgcggg gtggaaataa acctgcatgt
     1741 acatctggta gcgactgagg acgtagtctt cgatggcgtg catgccattg cgctgaaagg
     1801 cgataccatt ttcgatagga cgaatgactc ggaggattcg agtcaggtca aattccccat
     1861 aggatgctcc tgtaaaatag gagtcgcgca agagatagtc catgcgatct gcgtcaatct
     1921 gactagaaat gagctgcacg acctgcttat taggataggt atggtcaatg acactggcca
     1981 ccttttctgg gaaatcaggt gccacttgta gcaggacttg gtgaatctct gtctcaggat
     2041 tttgaataat ctcctgagta atggcttcat ggtctgtatc aaagagattt tcaaaagtat
     2101 gggagtaggc accatgccca aggtcgtgta ggagagcagc ggtcatggtc aagagagact
     2161 cggcaggatt ccattcctca ggatattttt cttcgaaaat ctctgtgatg cgtcgtgcaa
     2221 tttcatagac tcctagacag tgagagaagc gactgtgttc tccaccgtgg aaggtataac
     2281 tggaagttcc cagttgtttg atccggcgca aacgctgaaa ttcttttgta ttaatcaagt
     2341 catagatgat ttgattattg acatggatgt agttgtgaac agggtcacgg aatacttttt
     2401 cgttcatatg accattatag caaaatcctg ctctttttgg taaaatagta ggacaagaga
     2461 caagaaagag ggcctgatgt gaagattcgg atgcgaaata caattcagtt tgatgagcag
     2521 ttggaagtga ttgaccagct ttatgatgtg gaagtgcatg aaaaaggaga ttatagctac
     2581 ctgcttttct ataatgagga aaaggaaaaa gtagtgatta aatttcatgg tcaagaactt
     2641 gtgatgagtc gattttccaa tcccaagacc attatgcgct ttctaaagga tagtgatagt
     2701 ttagcctata ttcccacacc tatgggtatg caggagttta ttatccaaac gaatcgttat
     2761 caagtggatg ggcaaaagat tgaactagat tatcaactac aaaatcaaga gggacatccc
     2821 tttgccagct atcaattgga aattacttgg ggctaggctt atgtcttttt caaatttagc
     2881 tatctatctt cttggatcgg cttcctagtg tggtagatta ggctaatttt tggtataata
     2941 aaagttatgg aaatcgaaaa aaccaatcgt atgaatgcgc tctttgaatt ttatgcggcg
     3001 cttttgacag ataagcaaat gaattatatc gagctctact acgctgatga ttacagcctt
     3061 gctgaaattg ccgaggagtt cggtgtcagt cgtcaggctg tctatgacaa tatcaagcga
     3121 acagaaaaga ttctggaaga ttatgagatg aaattgcaca tgtactcgga ctatattgtc
     3181 cgcagtcaga tttttgatca gattttggag cgctatccca aggataactt tctgcaggag
     3241 cagatagaaa ttttaacaag cattgataat agagaataag aggaagaaaa atggcatttg
     3301 aaagtttaac agaacgtttg cagaacgtct ttaaaagtct acgtaaaaaa ggaaaaatct
     3361 ctgaatctga tgtccaagag gcaaccaaag aaattcgctt ggccttgctt gaggccgacg
     3421 ttgctttgcc tgttgtaaag gactttatca agaaagttcg tgagcgtgca gtcgggcatg
     3481 aggtcattga tacacttaat cctgcgcaac agattattaa aatcgttgat gaggaactga
     3541 cagccgtttt aggttctgat acggcagaaa ttatcaagtc acctaagatt ccaaccatca
     3601 tcatgatggt tggtttacaa ggggctggta aaacaacctt tgctggtaaa ttggccaaca
     3661 aactcaagaa agaagaaaat gctcgtcctt tgatgattgc ggcggatatt tatcgtccag
     3721 ctgccattga ccagcttaag actttgggac aacagattga tgtgcctgtc tttgcacttg
     3781 gaacagaagt accagctgtt gagattgtac gtcaaggttt ggagcaagcc caaactaatc
     3841 ataacgacta tgtcttgatt gatactgcgg gtcgtttgca aattgatgag ctcctcatga
     3901 atgagcttcg tgatgtgaaa gcattggctc aaccaaatga aattctcctc gtcgttgatg
     3961 ccatgattgg tcaggaagca gccaatgttg cgcgtgagtt taatgctcag ttggaagtga
     4021 ctggggtcat ccttaccaag attgatggcg atactcgtgg tggtgcggct ctatcggttc
     4081 gttacatcac tggaaaacca atcaagttca ctggtacagg tgaaaaaatt acagatatcg
     4141 aaaccttcca cccagaccgt atgtctagcc gtatccttgg catgggggat atgctcactt
     4201 tgattgagaa agcttctcag gaatacgatg aacaaaaagc ccttgaaatg gctgagaaga
     4261 tgcgcgaaaa cacctttgat tttaatgatt tcatcgatca attagatcag gtgcaaaata
     4321 tggggccgat ggaagacttg ctcaagatga ttccaggtat ggccaacaat ccagcacttc
     4381 aaaacatgaa ggtggatgaa cgccagattg ctcgtaaacg tgccattgtg tcttcgatga
     4441 cacctgaaga acgtgaaaac ccagatttgt taaatccaag ccgtcgccgt cgtattgctg
     4501 ctggttctgg aaatacattc gtcgaagtca ataaatttat caaggacttt aaccaggcta
     4561 aacagctcat gcagggtgtt atgtctgggg atatgaataa aatgatgaag caaatgggga
     4621 ttaatccaaa taaccttcct aaaaatatgc caaatatggg aggaatggat atgtctgccc
     4681 ttgaaggaat gatgggacaa ggcggtatgc ctgacttatc agctctcgga ggagcaggaa
     4741 tgccagatat gagccagatg tttggtggcg gtttgaaagg taaaattggt gaatttgcca
     4801 tgaaacagtc catgaaacgt atggctaaca aaatgaagaa agcgaagaag aaacgcaagt
     4861 aagacaagag attgatgcaa atctcatttg atgagaaaat aaagtaaaaa tttctatgat
     4921 aaaacgtaca gtatcaaggt tttagaatat ctgatattat acgttttttg attttaataa
     4981 ttttgttaag acacttttat tatgatatac gaaaaatcca ctcagctgtc tgagtggatt
     5041 aggtgtattt agactcttag tctttatttt cgtatggcaa gatcaagttc aagatgattc
     5101 ctgtcatggc tgaaagggca gtacctgaaa gtgtaactgg accaagttta aggatagctc
     5161 ctccaagtcc aagaaccaac atagcacttg cgatgatgag gtttcgcatt tgagcgaaat
     5221 caacacgttc tttaatcaag actttcaaac cattgctggc gataacccca tagagaagga
     5281 ttgacatacc accaagtaca gcgtttggaa tagttgaaat caaggcagtg aatttaccaa
     5341 ggaagctgag ggcaatcgcg atgaaggcag cgttacggat aactgagaca gaagcgatac
     5401 gagtcatacc gataacccct gtattttctc cgtaagttgt attggctggt ccaccaagga
     5461 aggcagaaac agaagttgcg ataccgtcac caataagagt acggtgaaga cctggttctt
     5521 ttaagaattg acggccacag atttgaccca aaacagtatg gtctccgata tgttcagaaa
     5581 ttgttacgat agcgattggc aagatagcga tggcttctgg accaaagtaa agattgtact
     5641 ctttaaaggc accacctgtg ctaaatggca agtagaaacc aggaatttcg aaccagttgg
     5701 ctttaagaac tggtgtaaag tcaaccaagc caagagttag tgcgaaaagg taaccaccga
     5761 taatggcaaa gaggaatgga atgattcgta ggaagccttt tccttttgta ttgataaagg
     5821 cagcaattag gaaagtaaca acggctacca gagcattttt ccaatttccg tctgctacaa
     5881 gacctgcatt ggtaacagct gaacctgcaa gtccaagacc gataacgatg atcataggac
     5941 cgataatgat tggtggcaag agtttatcaa tccattttgt tcctacaaat cggatgctgg
     6001 tagcaacaag gacatagacc aaaccagtca agataacccc tgtttgggca gcagatacat
     6061 ccccccccat ttctttcata gccagtgaca tagctgtgat aaaggcaaat gaagaaccta
     6121 gataaactgg aactttaaaa ccagtagcaa tcatgtagat gagtgttcca acacctgaag
     6181 caaaaagggc aacagataca ggcattccca aaatcaatgg taccaagatg gtcgcaccaa
     6241 acatggcgaa aacgtgttgg aagctaagga gaattccttt tccatccgaa ggacgttggt
     6301 caacgtctag taacaaatca acagttgatt cttgtttcat ataaacctca cttttgggca
     6361 tcagaaaaga gcccattatt ttacagcaat aggccctcag agtaagactt ctctaaaatc
     6421 tagactttaa agagtttcaa atatttctgc gtcttcgtca cctcacggga tgacattaaa
     6481 aacctttgct taggatagta tagcagaaaa aaatgatttt gtaaatcatt ttttcccgag
     6541 cctagaaata aaagagcgag gctgattttg taaatcattt tttcccgagc ctagaaataa
     6601 aagagcgagg ctgattttgt aaacatttgt ttactaaaaa atttaaagtg ggcgtttatt
     6661 tggcatacca gccttacgtg gatatttatt tggtgtttct tttttctttt ctaccactgt
     6721 gatatagcgc ggatctctat tcggtagggc gtagctgaga ttgtcttcga ccttactaaa
     6781 aaggagattg agggcattct tagcttctaa caactcctca ggcgcattgc tagccttgag
     6841 tgctaatagt ttgccaccaa ccttaaggta gggaatagtc aattcagata ggacctgcat
     6901 acgggcaacc gcacgagctg ttacaaaatc atattgagca cggaagttct tgtcttgggc
     6961 aaaatcttcg gcacgtccgt ggtagaaatg aactccgttc aaatccagtt cttgagccaa
     7021 gagttgtagg aagttgatgc gcttattgag tgaatcaata atggtcacat ctaactccgg
     7081 atagagaatt ttcattggta gactaggaaa tcctgcccca gccccgatat caagaagttt
     7141 gatagtttca ttgggaatca aaccttgaag aatgggtgca atcgaatcgt aaaaatgttt
     7201 gagataaact tcttccttgt ccgtaatcgc cgtcaaatta atcttctcat tccactcgac
     7261 caagagctca aaataacgtt caaattgttc tttttgctgg tccgaaagtg gaagattctg
     7321 ctcggcaagc aagttgtaaa atgtttctgg tttcatagtt atttccttct ttagtatcat
     7381 cttattttac cacaatcatt aaaaatttga tatactggta ctaatctaaa agtagaaagg
     7441 acttatatca tgacttggat tattcttgga gttatcgctc ttattgttat ttttgtgatt
     7501 gttagctata acggtttggt taaaaatcgt atgcaaacca aggaggcttg gagtcagatt
     7561 gatgttcagt tgaaacgtcg aaatgacctc ttgccaaact tgattgagac tgtaaaaggt
     7621 tatgccaaat atgaaggttc tacccttgaa aaggtggcag aactacgtaa ccaagtggcg
     7681 gcagcgactt caccagcaga agctatgaaa gccagtgatg ccctcactcg tcaagtttca
     7741 ggtatttttg cagttgcaga aagctatcca gatttgaaag ctagtgctaa ctttgttaaa
     7801 ttgcaagagg agttgacaaa cacagaaaat aaaatttctt actctcgtca actctataac
     7861 agtgttgtca gcaactacaa tgtaaaatta gaaactttcc cgagcaatat tatcgctgga
     7921 atgtttggat ttaaagcggc agatttcctt caaacacctg aagaggaaaa gtcggttcct
     7981 aaagttgatt ttagcggttt aggtgactaa gatgttgttt gatcaaattg caagcaataa
     8041 acgaaaaacc tggattttgt tgctggtatt tttcctactc ttagctcttg ttggttatgc
     8101 ggttggttat ctctttataa gatctggact tggtggtttg gttatcgcac tgattatcgg
     8161 ctttatctac gctttgtcta tgatttttca atcgacagag attgtcatgt ccatgaatgg
     8221 agcgcgtgag gtggatgagc aaacggcacc agacctctac catgtagtgg aagatatggc
     8281 tctggtcgct cagattccta tgccccgtgt tttcatcatt gatgatccag ccttaaatgc
     8341 ctttgcgaca ggttctaatc ctcaaaatgc ggctgttgct gcgacttcag gtctactagc
     8401 tatcatgaat cgtgaagaac tagaagctgt tatgggacat gaagtcagtc atattcgtaa
     8461 ttatgatatc cgtatttcga ctattgcagt tgcccttgct agtgctatca ccatgctttc
     8521 tagtatggca ggtcgtatga tgtggtgggg tggagcaggt cgcagacgaa gtgatgatga
     8581 ccgagatgga aatggtcttg aaatcattat gctagtggtt tccctactag ctattgtact
     8641 ggcacctctc gctgcaacct tggttcagct cgctatttct cgtcagaggg aatttctggc
     8701 agatgcatct agtgtcgagc tgactcgcaa tccccaggga atgattaatg ccctagataa
     8761 gttggacaat agcaaaccta tgagtcgcca cgtcgatgat gctagcagtg ccctttatat
     8821 caatgatccc aagaaaggtg gggggttcca aaaactcttt tatacccacc cacctatctc
     8881 agaacggatt gaacgtttaa aacatatgta aaatgaaggc tggaaaaaag tctttaaaat
     8941 ctgaaaaacg cataatatca ggtgtgaaaa cttgatatta tgcgttttac tatgggaaga
     9001 tttacttctt tttctcctaa aattgtgttt ttgccccacc tatctgctat gttgcaaatt
     9061 tataaatctt ctaaattaac tagcttcaaa ccgtgttctg tcatgcgata gatgatcgag
     9121 cagacttctt ttaagaaacg acggtcatgc gaaacagtga tgagaccgcc aggataagtg
     9181 gcaaagagtt ttctgatttg gggttgagaa gtgggagaaa agtttcgtgt gggttcatcc
     9241 agcaggagaa agtttggttt gcgcaggact aaatccaaaa gcaggagttt tccctgttgt
     9301 ccgccagata aggagcgaat ttgatgctgc atttctggat aactgaaatt gagactagct
     9361 aggtgagatt ggattttctg tagttcctct ttttccccag ttttactgag ataggctatt
     9421 ggggataaat ccaattgcag ttttttgtgg taatcttgtg gcataaaacc aagtgaaatc
     9481 tctcttttat cattcagaag tctctgtaac ttggctaaca gagttgattt cccaacacca
     9541 tttggcccga taataccgat tttttcttgg ccacggacag ttagttgtag tttttgaacc
     9601 aaaactcggt cgtcaatgga caaattttct ttttccagtt ggactaagac tttagaagct
     9661 ggtaatggtt ggatgtctga aaagaaaagt tggatttgtt cctcttcaag tggtttttga
     9721 gtcatggact gagctgcctt ttcgtagcgt ttttcttgtg agaggacagt tttcatcttt
     9781 ttagccaata ggcgaccggc agtactatct ttggtagctc gaagcgcagt ttctacattt
     9841 tgcttaactc tccgatgttt ttccatggtt ttatcgtagg ctctttggtt gttagcagct
     9901 tgctgacttt gtttggcaaa attagccttt ctctgctcac tatagctatc atagtctaaa
     9961 tgctctacta gcgtttccgc ttctttacgg tgtttgacca gtcgcaagtg aacaatagtg
    10021 tctgccgttt cagaaagaaa gtcttcatca tgggaaatga aaataacggt ttgcctggtc
    10081 ttttgaatct ggccttttag ccaatcaact gtctcaaggt ctaggtcatt tgaaggttca
    10141 tctaaaaata gaatctcaaa gggtttggct aactcatgga taagctgaat tttcaaagct
    10201 tctccccctg atagattgcc aatctcttgg tcacttgcga aacgattgct atcaaaatgc
    10261 aattcctccg ccaaacgata gaggatactg tagtctaaat caatagaatc taaaaagaag
    10321 tagtcgtgta aagttttctt tttcagctcc tcgggaagtt tttgaggaat gtaggccagt
    10381 gactgatagt cagattggat gtctcccttg atagtgaaat cagacaaatc ttcccccatt
    10441 aaagttttaa gtaaggttga tttaccattt ccttcttcgc caataatagc taccttttcc
    10501 ccgtcttgta tggtcatgct taggtcagag acaaggtctc gtaaatcttt gttttgtgtg
    10561 atggtcaggt gattgatttt tatcatacta ttgccctttc tagaatgtcg atagtgcata
    10621 acaaaaagct ctactttacc tagtagagcc caaagtctat gtcaaaaaaa ctctattaac
    10681 atgccagaaa aagagcataa aagagcacac aaaaagagac aaaaacaaga tctactaaat
    10741 aaaggttctt tatttgatag acttagttgt tcatgtctcc cttacctccg agtattagta
    10801 tctttatatt atacctttaa ggaaattttg tcaacagaaa actggaaatt ctatgctcta
    10861 aaatccaaaa acaggtccat
//
LOCUS       DAVKFH010000065        10797 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKFH010000065 DAVKFH010000000
VERSION     DAVKFH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.065"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            195..692
                     /locus_tag="VW006_001761"
     CDS             195..692
                     /locus_tag="VW006_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9380573.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            692..2347
                     /gene="dnaX"
                     /locus_tag="VW006_001762"
     CDS             692..2347
                     /gene="dnaX"
                     /locus_tag="VW006_001762"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9380574.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2375..2569
                     /locus_tag="VW006_001763"
     CDS             2375..2569
                     /locus_tag="VW006_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9380575.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2719..3489
                     /gene="sufC"
                     /locus_tag="VW006_001764"
     CDS             2719..3489
                     /gene="sufC"
                     /locus_tag="VW006_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9380576.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEGDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3517..4779
                     /gene="sufD"
                     /locus_tag="VW006_001765"
     CDS             3517..4779
                     /gene="sufD"
                     /locus_tag="VW006_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9380577.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4790..6016
                     /locus_tag="VW006_001766"
     CDS             4790..6016
                     /locus_tag="VW006_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9380578.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6003..6443
                     /locus_tag="VW006_001767"
     CDS             6003..6443
                     /locus_tag="VW006_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9380579.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6497..7909
                     /gene="sufB"
                     /locus_tag="VW006_001768"
     CDS             6497..7909
                     /gene="sufB"
                     /locus_tag="VW006_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9380580.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8108..9349)
                     /locus_tag="VW006_001769"
     CDS             complement(8108..9349)
                     /locus_tag="VW006_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9380581.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9452..10561
                     /locus_tag="VW006_001770"
     CDS             9452..10561
                     /locus_tag="VW006_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9380582.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3197 a         1973 c         2340 g         3287 t
ORIGIN      
        1 atatgattct ttctaatgag ttgttttgtt gcttttcatt ataggtcata tgggactttt
       61 tttctacaca aaataggctc cataatatcc ataggggatt tacccactac aaatattata
      121 gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg ctcctttttt
      181 gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg ttaaatgaag
      241 aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc aacgccagtg
      301 ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg ttcgatggaa
      361 aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt gcactaggca
      421 agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga gatgtgacga
      481 cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg gtgccgatga
      541 tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt gaggattacg
      601 atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa aagacaacat
      661 gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc gaaaatatag
      721 aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc ttaaacaagc
      781 ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg gaacgggaaa
      841 aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag tgggtggcga
      901 accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag aagatgtcat
      961 tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc gtgataaatc
     1021 tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg ttcacatgct
     1081 gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac agaatgtagt
     1141 ctttattttg gccactactg aattgcacaa gattcctgct actattctat cccgtgtgca
     1201 acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc actatatctt
     1261 agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca ggcgggcgga
     1321 aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga cacagggaaa
     1381 tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat cagccttgga
     1441 tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt gcttgaatct
     1501 tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc actatttaag
     1561 agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag tctttgtaga
     1621 aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa cagtgagttt
     1681 agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga ccgtccgttt
     1741 ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa ttgctacgct
     1801 gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg ttcctaaaca
     1861 agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg tcgatcgcaa
     1921 taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac gtcaaaatct
     1981 aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc cggacaaggc
     2041 tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc ttgcttttga
     2101 gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca tgtttggtaa
     2161 tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca tggaggaatg
     2221 gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg aaaaagaagt
     2281 agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga aggtagagga
     2341 agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat ttattattat
     2401 ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga ctggccgcta
     2461 tattatggca gccttttggg caattttact ctttagaaat ttccgagtca gttatgtgat
     2521 gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc cctcagcttc
     2581 cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta tatttataga
     2641 atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt aaaagttttg
     2701 aaaaagaaag gaactatcat gtcagtatta gagattaaag atcttcacgt tgagattgaa
     2761 ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat tgccgctatc
     2821 atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg aaatccaaac
     2881 tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga gttggaagtg
     2941 gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga aatccctgga
     3001 attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaagg tgatgagaag
     3061 atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct caacatgaaa
     3121 gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa aaaacgcaat
     3181 gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga gattgactca
     3241 ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat gcgtggtgaa
     3301 ggttttggtg ctatgatcat cactcactac caacgtcttt tgaactatat cacacctgat
     3361 gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga attggctgcg
     3421 cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga ctacaaggaa
     3481 gaattgtaat ttcctcgtat cttttaggag aagtaaatga ctagagaaaa tattaaactt
     3541 ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa agcttttgac
     3601 aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg ttggaatctg
     3661 ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt cacagcttta
     3721 gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca aactccagtt
     3781 gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga agaaattcca
     3841 gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa gttggcggct
     3901 taccacacag cttactttaa cagtggtgct gtactctata ttccagataa cgtagaaatc
     3961 acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc gtttaacaag
     4021 catattatga ttatcgctgg taaaaattct aagattagtt atctggagcg tttagagtca
     4081 cgcggtgaag gaagtgacaa agcaactgcc aatatcacag tggaagtgat tgcacgttct
     4141 ggtgcgcaag tcaagtttgc tgctattgac cgtctaggtg aaaacgtcac tgcctacatt
     4201 agccgtcgtg gtaaattagg caacgatgca agtattgact gggctatcgg tgtcatgaac
     4261 gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag ccatgctgac
     4321 ctcaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac tcgtgtaact
     4381 aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct tgaaaaagca
     4441 actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc agatgcccaa
     4501 caagagagcc gtgttctcat gctttcagac caagcgcgtt cagatgctaa cccaattctt
     4561 ttgattgatg aaaatgacgt cactgcagga catgcagcct ctattggtca ggtagatcca
     4621 gaagatatgt actacctcat gagtcgtgga ttggataagg caactgcaga gcgtttggtt
     4681 gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt tcgtgatgaa
     4741 atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta tgttagatgt
     4801 agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg aacctctggt
     4861 ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag ctattaacag
     4921 ttactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag cggaacgagc
     4981 gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg caggctctac
     5041 aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg cacgctttgc
     5101 tgaggaaatt ctcactgagg gagaccaggt cttgatttca gtaatggaac accattctaa
     5161 tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct atgtctatct
     5221 taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata aggttaaatt
     5281 tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca aggaaatcac
     5341 tcaattagcc caccaagttg gggcaatcat ggtagtggat ggtgctcaat ctacacctca
     5401 tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg gtcacaagat
     5461 ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg agcaaatgtc
     5521 tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatctg ctagttggaa
     5581 ggaattgcct tggaaatttg aggctggaac gccaaatatg gcaggagcta ttggacttgc
     5641 gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc atgaacagga
     5701 attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca tttacggttc
     5761 tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc tccatcctca
     5821 cgatcttgcg acagctctgg attatgaagg agtggctgtt cgtgctggtc accattgtgc
     5881 gcaacccttg cttcagtatt tggaagtccc agcaacagct cgtgcaagtt tttatatcta
     5941 taataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa aggagttttt
     6001 caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac cattcgaaaa
     6061 atccacatca ccaagggaag ttagaagacg ctgagcaaat cagtctcaac aatccgactt
     6121 gtggagatgt catcaatctc tctgtcaagt ttgatgcaga ggaccgtttg gaagatattg
     6181 cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg acagatgccg
     6241 ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct gaaatggttc
     6301 aagggcaaaa agatgagcgt caagaccaac ttggagacgc ggcattcttg tcaggtgttg
     6361 ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt aagaaaacaa
     6421 ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta ttagaaatga
     6481 agaaagaaag gatattatgg ctgaagaaag agtagaacca aaaccaattg accttggtga
     6541 atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa aaggactcaa
     6601 cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga tgttggagtt
     6661 ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg gagcagactt
     6721 gtcagagatt gactttgatg acttaatcta ctaccaaaaa ccatctgaca aaccagcccg
     6781 ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg ggattccaga
     6841 agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag tggtttacca
     6901 caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag attccgcact
     6961 caaggaatac ccagacttat ttaatcaata ctttgcgaag ttggtaccgc cgacagataa
     7021 caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct acgtgccaaa
     7081 aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg aaaatatagg
     7141 tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact acgtagaagg
     7201 atgtacagca ccaacatatt caagcaatag cttacacgct gccattgtag aaatttttgc
     7261 tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata acgtctataa
     7321 cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga ttgatggaaa
     7381 cttgggtgcc aaaacgacta tgaaatatcc atctgtttac cttgatggag aaggagcgcg
     7441 tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca cgggtgctaa
     7501 gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca tcgctaaagg
     7561 tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta agaaatctgt
     7621 ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag atactattcc
     7681 atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag tatctaagat
     7741 ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg aggcaactga
     7801 aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg aatacgcagt
     7861 tgagctgaac cgcttgatta gctatgaaat ggagggatca gttggataaa atttgatttt
     7921 atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg tatggttact
     7981 gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg cggctagctt
     8041 cctagtttgt tctttgattt tgaagatgaa cttgtatcaa aaaatcgcgg tttaaaatcg
     8101 cgatttttta taatttctcg ttaacaaagc ggacaaactg attccaccaa acttttaaga
     8161 agaaggcttt ttcaattttc ttgtctgcta ccatttcgaa actagggcgc tctgtggtga
     8221 tgtaaccttg accaatcaag tccttgtctt cataagtcaa atggccaacc actgttccag
     8281 cttcaagtgg tgctgggatt gctttggaat caggtgtgaa ttgaacagat tgggaagatt
     8341 gattcccaac acgttctatt agatagatat cctctggagc cactgcagtt actgtatctt
     8401 cttttccatc ttgtacaggg gttttgctat cttgataggc atcgccttgt tgaacgattt
     8461 tgcgaagtgt aaatgtagaa gaaatataat ccattaggga agatgtagct gtaaatcgag
     8521 cgtaaggatt attgtcttga tgatctgcat ttaaaacaac tgtgatgact ctcatgcctt
     8581 tttcgacagt agtaccaaca aaagactctc cagccttatc tgttgttcct gtttttagcc
     8641 catcaaaacc accacggtaa gcaggcatac cttctaacat gtagttggtt gaagtgattg
     8701 tcatcccagc aaaagtagaa gaaggttttt tggtgatttc taagacttgt gggtattttt
     8761 tgatgaggtt gcgagcaacg atagcgacat cataagcact aagcttattt tcctcatctt
     8821 ttttagaacc tgggtaaatg ttatccccta gagtttcatt gttaagacct gtcgtattga
     8881 caacagtggc atcctgaatt ccccattcca agagttttgc ccgcatcata tcgacgaaat
     8941 ctttttctga gccagcaatt ttctcagcta gggcaatagc ggcgctgttg gcactagata
     9001 ccagagttgc ttcaagcaac tcttcgacag tataattacg ggcctccata ggaatattac
     9061 tggcttcaga atttgtcgtc aattgataag gataatcaga aatatctaca ggagtggaga
     9121 gggtaatact tccgttttcc aaagcttcat agaccagata aacagtaatc aattttgtta
     9181 tggaagcaat ttcgacaggt tgcgttgcat ccttctcata gagaatttta ccagtatttg
     9241 cctcaacagc aatcgcatgt ttagcggcaa tggtaaaatc ttgagcaaca gcagtagaag
     9301 caccccctaa aagagagaca gttaacaaag ttaaaaatat ttttttcata gtagtcttat
     9361 tctatcataa agaaaaaaaa tattcttgct ttaataattc atctgttaag ctttttgaaa
     9421 atatggtaaa ataaagtgag ggaggtaact catgtttcgt agaaataaat tatttttttg
     9481 gaccacagaa attttactct taaccatcat cttttaccta tggagacaga tgggatcttt
     9541 gattaaccct tttgttagcg tgcttaatac aattatgatt ccatttttat tagggggctt
     9601 tctttattat ttgacaaacc ctattgttac tttcttaaat aaagtctgta aactcaatcg
     9661 tttgcttggt attttaatta ccttgtgtac tttggtctgg ggaatggtca taggtgttgt
     9721 ctatctctta cctattttga ttaatcagtt atctagtttg attatatcta gtcaaactat
     9781 ttatagtcga gtacaagact taatcataga cttatctaat tatcctgcgc tccagaattt
     9841 ggatgtagaa gctacaattc agcagttaaa cttatcctat gttgatattc ttcaaaatat
     9901 cctaaatagc gtatcaaata gtgtggggag cgtcttgtca gctcttatca gtactgtttt
     9961 gattttgatt atgactccag tttttttggt ttatttctta ttagatggac ataaattctt
    10021 gcccatgctt gaaagaacga ttctaaagag ggatcgcttg catattgcag gcttattaaa
    10081 gaatttaaat gcgacgattg ctcgctatat tagtggagtt tcgattgacg caatcattat
    10141 aggttgtttg gcttatattg gctatagtat tattggttta aaatatgctt tagtttttgc
    10201 cattttttct ggtgtagcca atttaattcc ttatgtgggg ccaagtattg gtttgattcc
    10261 tatgatcatc gcaaatatat tcactgatcc ccatagactg ctgattgcag tgatttatat
    10321 gcttgttgtt cagcaggtag atggcaatat cttatatcct cgaattgtag gaagtgttat
    10381 gaaggttcat ccaatcacga ttttagtttt acttttgttg tcaagcaata tctatggtgt
    10441 agttggaatg attgtcgcag tgccaaccta ttctatcttg aaagaaattt ctaagttctt
    10501 atcccgtttg tatgaaaatc ataaaataat gaaagaacga gaaagagaat tagctaagta
    10561 aaagtcagga gaaccctgat ttttctttac tggaagtggc ctttagatta gaagactgaa
    10621 aataagttaa agtcttaaac taattttcac agctaagaat agtagaagtt aatctgataa
    10681 aaatcgaaaa aaccagtgga attccgtgtc agggtaagtt ccactggttt tcatagtcta
    10741 ttaaagttcg aatgaaacct atttataata tattgaaact agaatagtac acctcta
//
LOCUS       DAVKFH010000066        10746 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKFH010000066 DAVKFH010000000
VERSION     DAVKFH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.066"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(105..1529)
                     /locus_tag="VW006_001771"
     CDS             complement(105..1529)
                     /locus_tag="VW006_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9380583.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="VW006_001772"
     CDS             complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="VW006_001772"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9380584.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4465..6423)
                     /gene="ligA"
                     /locus_tag="VW006_001773"
     CDS             complement(4465..6423)
                     /gene="ligA"
                     /locus_tag="VW006_001773"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9380585.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6516..7694)
                     /locus_tag="VW006_001774"
     CDS             complement(6516..7694)
                     /locus_tag="VW006_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9380586.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7774..8637)
                     /locus_tag="VW006_001775"
     CDS             complement(7774..8637)
                     /locus_tag="VW006_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9380587.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8640..10541)
                     /locus_tag="VW006_001776"
     CDS             complement(8640..10541)
                     /locus_tag="VW006_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9380588.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3233 a         2366 c         1908 g         3239 t
ORIGIN      
        1 gtttcaatct attataatac caaaaagttc gtattttttc tataattatt attattttat
       61 agcaaaaaaa taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa
      121 acaatggatt tgacatttgt catcgcttca atgctatatt taattccttg cactccagct
      181 ccagaacctt tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta
      241 atgtggactg tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt
      301 gtaaagactg atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct
      361 acactagcca cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc
      421 atatcttttg taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt
      481 ggtgtaagag cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg
      541 gctgaagcat tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt
      601 aatttagaaa cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca
      661 atgacacgtt taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt
      721 tgcttggcag catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca
      781 agttccaaca tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt
      841 gaacctgtaa agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct
      901 gaaccacgac ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct
      961 ttagccaaca agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc
     1021 cctgcaatca aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga
     1081 gcaatcgcta gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt
     1141 gctgcctcaa aaccaccacc ttccattgct tgtccagtga tacggagacc ttcctcagca
     1201 gcataacgaa tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt
     1261 gctacttctt tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct
     1321 gttttatgta aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct
     1381 gcacgcgcag cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc
     1441 aattcttctt gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt
     1501 ccatttacta aattctgata tcttgtcaaa ttcttgtcct cctgaaaatg attaagatat
     1561 attttaccaa attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca
     1621 caaacttgat ttttcaaagt atactcaata aaaataaaaa aaatccacta cgtcaaggcg
     1681 aggctaatgt ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa
     1741 taaaaaaact gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa
     1801 acaagggtgt gctactccaa tttcagccta ctataactgt catagattgc tgaaacaaag
     1861 tctaggtaaa agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag
     1921 gaggtcttgt tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg
     1981 ttctttctat tctttcaatt tcaatgaatg gattttttac taatactcat aactgggaat
     2041 ttgtctgtgt aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact
     2101 aatatcattt aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt
     2161 tcctgcattt tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc
     2221 tttgccgtga acttcataga taaggtagcc gctatgttca atcgcagaat gcacaaagac
     2281 atgatggtaa atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg
     2341 gatgacttga cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac
     2401 ttggttcaca ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt
     2461 caactcacca ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc
     2521 cataaaggcc atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat
     2581 gatttgctct gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt
     2641 gtcatgggct tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg
     2701 acttcctagg attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga
     2761 cttgatagca ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa
     2821 accaatattt ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc
     2881 tgttcccata tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct
     2941 ttgacgaatc atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc
     3001 gtcaatatta tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat
     3061 ttcgtgttca ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg
     3121 attcatacga taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa
     3181 ggtatggtta tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac
     3241 catcaccttc aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt
     3301 ttctggcgcg ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc
     3361 atcgtattct ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt
     3421 cttgatgtaa tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc
     3481 acccaagaaa gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc
     3541 acgaatatgc atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc
     3601 cgaaccgtgc ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga
     3661 acgtttgcca tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg
     3721 agggaattgg acttgatact gataagcctt acctaccaaa tcttcctcaa catccaaact
     3781 ccagacaccg attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc
     3841 aaaagtcttc caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc
     3901 tgtcgctgta ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa
     3961 ttctccttgg taaccccaat gatggtcaaa actagcactg ttaatggcct tatcaaaggc
     4021 aaaaggattt tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat
     4081 cctatcatcg ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat
     4141 agaatattct ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg
     4201 tgaacttggg tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa
     4261 atcaattcgt tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg
     4321 atggtaatga atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt
     4381 ctatttcact aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc
     4441 taaactctgt ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg
     4501 gataccaagt tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag
     4561 gtcggtcttt ttagaaatac tacctgtcac tttggcaccc agactttcga gtttactttt
     4621 agcttctgag cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa
     4681 ggccgcatcc gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa
     4741 ttctctgagc agaatttcag agccttctgt cgcaaaataa gtctgaagac ttttggcaat
     4801 cacgccacct agactttcaa tactagccac ttcctctgaa tctgcctgag acagattttc
     4861 aattgaatgg aaatattgaa gtaaaagctg actaaccttg cttccgacat gacgaattcc
     4921 caaaccaaat aagagcttct cggcagaatt ttcctttgat gcttggatag cctgatacag
     4981 tttagcagcg gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg
     5041 ataaatatcc gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg
     5101 accaaggcct gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat
     5161 ttgagcaggg caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa
     5221 gtcagagtta caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt
     5281 ggactctacc acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt
     5341 atcgtctttt cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct
     5401 aacagtcgta ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt
     5461 acggccaact gtccagtcaa ctgataagag ttgagcttct ttttcttcgg cagggaactt
     5521 gtaggctact gcccactttg gagccttaac tgtaaaacca agttcttctt gacttgctag
     5581 gtcgttgacc ttgattacca ctccatcaat atcgtaaggc agattttccc gttcctgtcc
     5641 tacttcttgg ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt
     5701 gaccacaaaa cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga
     5761 agggctggct tcttgataga gaaacgttgc aagattacgc ttggcaacta ctgctgtatc
     5821 caactgacgc agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt
     5881 ttcttggcgc acttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg
     5941 aactgtgata tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt
     6001 ttctgtgata ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc
     6061 cttttcataa gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg
     6121 atgagccact tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc
     6181 atcctgcaaa ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt
     6241 gccaccaaca cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa
     6301 ctcgaccaac tcacggtaaa ggcggtcata ctcactgtct gcaaccgagg gattatcgct
     6361 ggtatagtac tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt
     6421 cataagacca ttttaccata aaacaagccc tcctcacaaa cgagaagggc ggaaaaaaca
     6481 cttagtttga aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt
     6541 cgaacataaa taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa
     6601 gaagagagat gtacacttgg caagactgtc ataaatcctt ttgcaatagg cataaataga
     6661 atagctaagg taaaaattgt actcagtact cttccaagaa attcgctctc aaccttggtt
     6721 tgtacttgag taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt
     6781 ccagaaaagg aaagaaaagt tggaagtggt aatcccatca taaaaactcc gacacctgtc
     6841 aaagccagta aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc
     6901 ccaatgatgg aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg
     6961 taaagctgat tcgaaaaggg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg
     7021 gaagctacca gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc
     7081 ttgatatcta caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc
     7141 tcttttggaa ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt
     7201 agcgtcatat tgagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca
     7261 cctacaacct gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc
     7321 accacttcag ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagcctgc
     7381 acaatgttag caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata
     7441 gccagacaaa gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa
     7501 cggtctgaaa taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc
     7561 tgatacattc ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt
     7621 ccataatcat agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact
     7681 gcatagcgat tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa
     7741 ggagcttttt attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg
     7801 ttcctgataa taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctt
     7861 ttgcatctgc ttacagcctt ctttacactg tcctttttga tataaggcaa aaccttttaa
     7921 ataatggaaa acattacgct cataaagctt aatacctttg tcaataatct tctctgtata
     7981 agcctcaaaa tagttggcat tataaaaaga agaatgctct aaacaatgct ggtaacaatt
     8041 gagggccaaa atcaaaacta atctcttatg gcgactaatc tcttggtaaa attcctccct
     8101 ctccataact tctctaccaa tccgagtgac atagtctaca tcgtagaaac tatagaggtt
     8161 accgaaaaga atcaactcat acatggtcca ttcttctgtt ttgaagagat aatctgctac
     8221 cttatccaaa tcatcctgct tcatatcata agtcgcatct ctttgacaaa tcagaccttg
     8281 tagcaaaatc cagttcagct caaaataaag ggaagtcgtc gaactcttag acttttcaag
     8341 ttgttctctt tgaagctttt gaaaacctgc aatatcgttt gaatagtaaa gtgggataat
     8401 ctgtgccatc atagacacat gttcatgatt atgaaaattc cttgccttat ccatgaaatt
     8461 ttcgattgtt acatgaatgt tatccaaaat ctcaaagaaa cgggagactg ccaggtcaga
     8521 ctccccaagc tcaaagcgag ataactgaga ggtagagcag gattcgcctg ctgcttcctt
     8581 taaagaataa tttccacttg ttcgaaattc acgaaatact tttccaagat gttccatctt
     8641 tacacctgat ctgataattc ttcccactca agcatagctt cttcctgacg atggctgatt
     8701 ttgtccagct cagcctgtaa ttccatgagt ttgtcggcat cgtttgtttc caacatttgt
     8761 tcagaaatgg cttggctttg actttctagc tcttcaattt cagcttctag actttcgatt
     8821 tgtcgcatga gtttgcgaac ttctttttga ctttctttct gggcctgata gtcattgact
     8881 ggacttgctt cctttgcttg attgctagtt gaagcttcct cagtctgact catttctgct
     8941 gttgctttct tctcaacata gtagtcgtaa tctccaaggt agagagttga accattctca
     9001 gacaattcca aaacatgagt tgccacacga ttgataaagt aacgatcatg actgacaaaa
     9061 agcaaggtac catcaaagtc aatcaaggca ttttctagca cttccttact atcaatatcc
     9121 aagtggttgg tcggctcatc cagaatcaaa aagttattgt tttccataga caatttagct
     9181 aaaagcaaac gagctttttc gccaccagat agcatgccga ctgatttttt aacatcatct
     9241 cctgagaaaa ggaaggcccc aagacggttg cggatttcaa cttctggtgt cagtttgaaa
     9301 tcattccaga gttcatccag caccgtatta cttggtgtca gcttgctttg ggtttggtca
     9361 tagtaaccaa cctcaacatt agcgccaaag cgcttttcac ctttgataaa aggaatctgg
     9421 tccacaatag acttgataaa ggttgacttg ccgataccat ttggaccaac gatagcgaca
     9481 gcattcatct tacgaagatc taggttgata ggttgtgaca aaacttcccc gtcataacca
     9541 atagcagtat tttcaacagt caaaacaaca ttgcccgacg ttttttcaga ctggaaggtc
     9601 atgttggctg ctttcttgcc agcttcaggc ttgtccaaac gctccatttt ttctagttgt
     9661 ttacggcgag attgagcacg tttggtcgtt gaagcacgaa ctagattgcg attgacaaag
     9721 tcttccagag cagcgatttc cttctgttgc ttttcatagt tttttgcctc agtagctagc
     9781 ttttgctcct tcaactcaac aaaacgagag taattcccca catagcgatc caaggaatac
     9841 ttggtcaaat ctagcgtaat tgtcgcaacc ttgtccaaga aataacggtc gtggctgacg
     9901 ataatgaggg caccgctata gtttaccaag taattctcta gccaggagat ggtttcaata
     9961 tccaagtggt tagttggctc gtccaagacc aagagattgg gcttttcaag gagcatttta
    10021 gctagagcca aacgagtatt ttgaccacca gaaagctcag caattttcat ctgccacata
    10081 gactcgtcaa acttgaatcc attcaaaatc gctcgaatat cagcttcata ggtaaagcca
    10141 cctgcttggc gaaaattttc agataagcgg tcataatctg acatcagttt atccaaatcc
    10201 tcaccagact tttcacccat ctccagctcc atctgacgca gttgtttatc tgtccgacgt
    10261 aagtcatcaa agacatgaag catctcatca tagatggtat tttcagactc aaaacggcta
    10321 tcttgagcta ggtaagacag agaaatatct tttttcttat tgatttctcc actagttggc
    10381 tcctcttctc caactaaaat cttcaaaaga gtagacttac ctgcaccatt tttcccaaca
    10441 agggcaatcc gatctcgttc atcaacctgc aggttgatat tatcgaaaag aacctctcct
    10501 gcaaaagaac gttcaatttt attagcttgt aaaataatca tacaagtagt atagcatgtt
    10561 tccctaaggc attcaagata atcgtaagtc ttttagtaca acttttataa cataaaataa
    10621 actaaattat gtatatttta tattagattc cttcactatc ttgttggatt ttctaaccag
    10681 ctaatcttgt ttcaaatagt tatcgcacaa gcctattatt taattctttt cattatttac
    10741 gtacgc
//
LOCUS       DAVKFH010000067        10612 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKFH010000067 DAVKFH010000000
VERSION     DAVKFH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.067"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..268
                     /locus_tag="VW006_001777"
     CDS             <1..268
                     /locus_tag="VW006_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9380589.1"
                     /translation="ILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRL
                     IRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            complement(297..1511)
                     /locus_tag="VW006_001778"
     CDS             complement(297..1511)
                     /locus_tag="VW006_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9380590.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1667..1961)
                     /locus_tag="VW006_001779"
                     /pseudo
     CDS             complement(1667..1961)
                     /locus_tag="VW006_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2123..2575
                     /locus_tag="VW006_001780"
     CDS             2123..2575
                     /locus_tag="VW006_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9380591.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2610..3998)
                     /locus_tag="VW006_001781"
     CDS             complement(2610..3998)
                     /locus_tag="VW006_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9380592.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            4064..5026
                     /locus_tag="VW006_001782"
     CDS             4064..5026
                     /locus_tag="VW006_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9380593.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(5232..6242)
                     /gene="ccpA"
                     /locus_tag="VW006_001783"
     CDS             complement(5232..6242)
                     /gene="ccpA"
                     /locus_tag="VW006_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9380594.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLVKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6666..7265)
                     /locus_tag="VW006_001784"
     CDS             complement(6666..7265)
                     /locus_tag="VW006_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380595.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLAFQADVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7266..8363)
                     /locus_tag="VW006_001785"
     CDS             complement(7266..8363)
                     /locus_tag="VW006_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9380596.1"
                     /translation="MLERLKSIDYMYWASLIFMIFPILPVVTGEIPSWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERAEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVSPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8363..9100)
                     /locus_tag="VW006_001786"
     CDS             complement(8363..9100)
                     /locus_tag="VW006_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380597.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAMFSFPAVLHTDKINNWQKTLRHTPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHFVRGVDMPLGSWIGAAFLLIVGSVAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYILVFSAVLFGLTIYLQGHKENA"
     gene            complement(9102..9986)
                     /locus_tag="VW006_001787"
     CDS             complement(9102..9986)
                     /locus_tag="VW006_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380598.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9973..10167)
                     /locus_tag="VW006_001788"
     CDS             complement(9973..10167)
                     /locus_tag="VW006_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380599.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(10164..10295)
                     /locus_tag="VW006_001789"
     CDS             complement(10164..10295)
                     /locus_tag="VW006_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEU9380600.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKEFEE"
BASE COUNT         3221 a         2436 c         1939 g         3016 t
ORIGIN      
        1 tattctgtcc attactgcag ataatggttc ggagttcaaa cgattgtctg aggtatttcc
       61 tgaggaacat atctactacg cacatgctta ctcttcatgg gagagaggtt caaatgaaaa
      121 tcataatcga ttaattcgga gatggttacc taaaggaacc aagaaaacga ctccgaaaga
      181 agtagctttt atcgaaaatt ggattaacaa ctaccctaaa aaatgcttgg actacaagtc
      241 gccaagtgaa tttcttttgg gtggctaact tcaacttgaa atttgggatg caagccctat
      301 ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa ctcatcaaca
      361 cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc tcgaccatga
      421 accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc atcatcgata
      481 cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg tttaacggca
      541 gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc gtagattcgt
      601 ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt ttgtacgact
      661 tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag cccttcgata
      721 tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg gaaacctgct
      781 atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg tgccaagctc
      841 gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc atccgcaaag
      901 ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc cttaggataa
      961 agggctccag ttgggttatt tggattgata aggacaattg ccttggtatt ggaagtgatt
     1021 tttgacttga tatcgtcaat atctgggtac cattccacag cttcatcaca gatatagtga
     1081 acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc tggcattggc
     1141 actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat caactcactg
     1201 acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt cagctggcaa
     1261 tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata gccttcacta
     1321 tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa gccaaattct
     1381 gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat ccgcatggct
     1441 tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact agacttgtta
     1501 tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca ctagaaagag
     1561 aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa ccttgctaaa
     1621 ggcgaggtta agaacgtaga gatagcaggt attttgcaaa gacacatcac ataccaaaag
     1681 ataaacctag aaaaaagcaa gcctagaaag gagtcataaa gaagattaaa catgaggaaa
     1741 agcccccata tcatcaagta atcccaacgc ccacttcgtt ttgcaactag aaataagatt
     1801 agatggtaga gtacatgaaa aactaaaaat ccaataaaac ttggataaag aagcggtacc
     1861 agactctcta attgacagat catgatcact tctaccaaga ctgaaactag gataattcca
     1921 taataaagga aatttgattt ttgaaaagag tttttcatca ttctcccttc cttcacttct
     1981 ttcctgctat cctttctttt attttatcat atatttatat agagtttaag ccctaatatt
     2041 cttttgaaaa atactttttt tgctttactt ctatagtgaa aaggtttaca attatagtaa
     2101 gaaaatttca ggaggtttca ttatggcaca acgttaccaa aatatcatgg tcgcaatcga
     2161 tggttctaag gaagcggact tggcttttgt caagggagtt cattctgctc tacgaaacga
     2221 cgctaaactc accatcgcac atgtcattga cacacgcgct ctccaaagcg tatccacctt
     2281 tgatgctgaa gtttacgaag aactccaagt cgacgctgaa agtctgatga aagagtacga
     2341 aaaacgtgct aaagatgctg gggtagcaga tgttcatatc gtcattgaaa tgggaaatcc
     2401 aaagaccctg ctagcacgta ctattccaga tgccgaggaa gtggacctca tcctcgttgg
     2461 cgcaactggt ctcaacgcct ttgaacgcct cttggtcggc tcttcatctg aatacatact
     2521 ccgccatgct aaggtcgatt tgctggttgt gagagaacaa gaaaaaacct tataatcaca
     2581 aagaaaagga gcccctagct cctttttgtt tacgatttat ttctctcttt atggcgttcg
     2641 taagccttga gctggcgctg cagttccttt ttaatagcag gttctggagc atatttttct
     2701 tcccaattat ctggttttaa gattttatgg gtcactggat caaaatgagc cttgccatct
     2761 ggaaaaattt tccccatatt ggcctgatgg acaatatcaa aaatacgttc tgggtccacc
     2821 cccatcaaga caaaactgcc gtaggtgaag taaagcgtgt caatcaaggc atccacttgc
     2881 cctatcaaat cttgctgagc aggtgtcttc ttggctactt tacctgctgc cttatcaagg
     2941 gcctgatgaa gttgcgatac agcttgacca aaatcttctt cagaaggact ggctgatcga
     3001 acaaactcca ccaattcttc tattttaaaa ccagccctat gggttgcacc ctctaaatcc
     3061 caagctcgag gttcttcttg ggttcgttca tccatcatgt ggtggaaggt cttgacctta
     3121 ttgaaatgat agtcacggct gacaaaaact ttttctgaag acaaaacacc aaaatgttcc
     3181 aaggccttgt ggatcccatc ttgttgattg ctagtggtaa tgtgcttggc agcttccttg
     3241 acactgctac taccatttcc catagcgacc gacataccga cgccagccag catttcaagg
     3301 tcattatctg agtcaccaaa agccatgact tggttgaggt caaagccata ttctttccca
     3361 actcggcgaa tgccttctaa tttagaattt ccctgattga tgacatccga tgcaaaagga
     3421 ttgctacgtg tcaatttcaa gtcttcaaaa tcagctgccg ccttctcaga ttcttctggt
     3481 gtcatcagca tcaaaacttg gtaaataggt tgattcatca ggtgaagcag gtcctcttcc
     3541 ttttggggaa caaccttgct gaccatgcga ttaaaagact gactcaccgt ccgagttaaa
     3601 acagagggaa cgaagcgact aattcgttgg gaaaaagaac ccagaccaaa ggacatgatt
     3661 ttagaaccca acatggcatc cttggtccct aaggcaatct ccgtgccctc ttttttagca
     3721 tagctaatta gatggcgcaa atgtaacttg gaaatagggc tcgtgaacaa gactctgtct
     3781 ttactaaaga tatactggcc attataggtt accgcaaaat ccagatccaa atcgtccatc
     3841 aattccttaa caaaaaaagg tcctcgccct gtcgctacgc caactagtac cccttgttct
     3901 ttgacaatct taatcgcatc cttagtggat ttcaaaacac tcttgcgatt gttgaccaag
     3961 gttccatcga tatcaaaaaa aacagctttg acttccatcc tatcccaatc tccccttttg
     4021 tgatacaatg attataccac atttcagaaa gagtgagtaa atcatgccta agaaaatcct
     4081 tgttttacat acgggtggaa ctatttccat gcaggccgat gcttctggcg ctgttgtgac
     4141 gagttcagat aatcccatga accatgtgtc caacccactt gaaggaatcc aagtccacgc
     4201 cttggacttt tttaaccttc caagtcccca tatcaaaccc aaacatatgc tggtcctcta
     4261 ccagaaaatt aaagaggaag cagataacta cgatggagtg gtgatcacac acggaaccga
     4321 tactttagag gaaacagcct atttccttga taccatggaa gttccccata tgcctatcgt
     4381 tctaacagga gccatgcgta gctccaatga actcggtagt gatggtgttt ataattacct
     4441 aagtgcttta cgagtggcca gcgatgacag ggctgctgac aaaggagttt tggtcgttat
     4501 gaacgatgaa atccacgctg ccaagtatgt caccaaaaca catacgacta atgtcagcac
     4561 cttccagact ccaacacatg gcccccttgg tctcatcatg aaacaggaaa tcctctactt
     4621 caaaacagct gaacctcgtg ttcgctttga ccttgatcac atacaaggtt tagtccctat
     4681 catctcggct tatgctggta tgacagatga gctgattgat atgctggatt tagaacactt
     4741 ggacggtttg attatccaag ccttcggagc tggtaatatt cccaaagaaa cggctcaaaa
     4801 attagaaagc cttctgcaaa aaggaattcc agtcgctctg gtatcacgat gctttaacgg
     4861 tattgccgag cctgtttatg cctaccaggg tgggggcgta cagttgcaaa aagcaggcgt
     4921 tttctttgtt aaagaactca acgcccaaaa agctcgcttg aaactcctca tcgccctcaa
     4981 tgccggacta acaggacagg ctttgaaaga ctatatggaa ggctaatact cttcgaaaat
     5041 ctctgcaaac cacgtcacgt cgccttaccg tatgtatggt tactgacttc gtcagtttca
     5101 tctacaacct caaaacagtg tgttgcgcag cctttaacca gccgcctagt ccgctctatg
     5161 gtattcatta agtcaacatc tcttgtttaa gagcacaaaa tcaggaaatc ttctcgattc
     5221 cctgattttt tctatttacg ttttcgtgtt gagctacgtt ctgtcaaacc atgaggtaag
     5281 agaacttcac gttcttccaa ctcttcctta tgcataatct tggtcaacat acgcatacta
     5341 atggcaccaa ggtcataaag aggttgggca atcgttgtca agtttggacg ggtaaagcgt
     5401 gagatttgtg aatcatcact agtaataatt tcaaaatctt ctggcacaga aacaccctta
     5461 tcagccaaac cgttcaagac tcctgctgcc aactcatcac ctgtcacaac tgctgcagtt
     5521 gcatttgatg aaatcaaacg ctctgctaag gcgtaaccat catcatagct atatttagat
     5581 tcaaatacca aaccctcact ataagtgatt cctgcttttt tcaaggtttc cttgtagcca
     5641 actaaacgaa ccttaccatt gatgtcatcc actagcggac cgctaacgaa agcaatacgc
     5701 tcattttctt taacaaggta actcactgca tcaattgttg cttgcttata gtcaatattg
     5761 acacttggca actggtgctc aacatcgaca gttcctgcga gaacaatcgg agtacgtgaa
     5821 cgcgaaaatt ctgagcgaat tttatctgtc aagtgataac ccatatagat aatgccatct
     5881 acctgctttg aaaagagggt attgacaaca gaaacttctt tctcgttatc ttcatcgcta
     5941 ttagctagga caatattgta cttgtacatt tctgcaatat catcaatccc cttagccaaa
     6001 ctcgaaaaat aaccattggt aatatttgga atcacgacac cgacagtggt tgtcttttta
     6061 cttgcaagac cacgcgcaac tgcatttgga cgataatcca aacgatcaat tacctctagc
     6121 acttttttac gggtattctc ttttacattt ttattgccat tgaccacacg gctgaccgtc
     6181 gccatggaaa cacctgcttc acgagcgaca tcataaatgg ttactgtatc atctgcattc
     6241 attccttttc ctgtcctttc tatctcacac attcttttac aagtagaggt gctgattgaa
     6301 gctctatatc tacttacaaa agtgaagatg tgaaaatttc gttttcatat ttctacttat
     6361 tccattctat cactaattgt aaacactttc aagtgttttt tgaagattga ttgaaaaaat
     6421 ttcatagaaa acctaggttt agctccttgc taccacctta gactaaacaa aaaggaggaa
     6481 actaagccct cctaaagtta tagtaaaatg aaataagaac aggataaatc gatcaggaca
     6541 gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatcta
     6601 ctataggtat tgttccattc actaccgtca attttagcac atagtcttca taaaatatta
     6661 tatcatcata accaaccaga ttcttttgcg atattagctg cctctgttcg attaccagca
     6721 tccagttttg aaagaatatt ggtgacatag tttcgaatag ttccgtttga gaggtaaagc
     6781 tgatctgcga tttcttggtt ggataagccc cgggcgattc cctttaagac agcaatctct
     6841 tgttctgtca acggattggg acgggtcatc accacttcca tcaattcagg cgaatactcc
     6901 ttgcgtcctt cgaggacggt gtgcaaggtt tgcatgaggt ctgcaatgct tcgttccttt
     6961 aatacataag catctactcc agccttgacc gcacgttcaa aataccctgg gcgcttgaag
     7021 gtcgtcacta caaccacctt tgtttcgagc ttttctgctc gtatccactc caagacttcg
     7081 agtcctgtct taacaggcat ttctacgtca aggatggcga tatctacaga ctccttttct
     7141 aatagttgaa ttgcttcttg accattcttg gcttgtaaga cagactctac atctgcttga
     7201 aaggcaagca actggcacat ggcatctcgc aacatacttt gatcttctgc gactaatact
     7261 ttcatctact ttctctcctt ataaggtagt cgaacttgca cttccgttgg atgtttctga
     7321 ctgattacac ttacttctcc tgaaaatgga gaaacacgat ctcggactgt atggagctca
     7381 tctcctttta gaaaagcaaa gccgcagcca tcatcactta ctgttaaaat gagttctttc
     7441 tctgcccgtt ctaatttcag gtaggccttc gacgctttgg catgtttgat aatattggtc
     7501 accaactcaa gcaaaatcat ggaagccgtt gactccaatt cttgagtaag actagcagta
     7561 tctagttggt tatccgtttc cacctcaatt ccagcaattt ctaacatctt tttcacagtc
     7621 tctagttcgg atgtcaaagt tctagactta agattttcca caatggttcg cacttcattc
     7681 atggaatcct tactgatctg gtgaatttct tttaattcct tttccacctg tggataagcc
     7741 tccatctgaa ataactgcaa ggctaaatct gtcttgacac tcaacatagc aaaggtatgt
     7801 cccaagctat catgcaaatc ctgaccgata cgactgcgtt cattttcagc aagcaataga
     7861 tttatctggg cattttgctt gacctgagct tctttcaaat cctccacaat acgaatccga
     7921 accaagccaa aagtcattaa atcgacaaaa gcaagaatta caagtagata gaatagaaac
     7981 tcaacttcga ttctctgaaa aatcaacagt tggcccacaa caaggacttg agcaagaaga
     8041 aaagtccaga catgtaaaga ctttaaacca cctacgctga aatgataact taagagattg
     8101 gataggaaaa agaaaaacca gatataatta acagcaacaa aggcagtatt cccaactaca
     8161 taagtcagca tgaggcccca atatagccaa gataggcgct ggctcttagt tgttaaaaca
     8221 cccaaatacg ccactacaaa tagaatatca atcaataaat gccagctagg aatctcccca
     8281 gtcactacag gtaggatggg gaaaatcata aaaattaaac tggcccaata catatagtct
     8341 atgcttttta gtctttcaag cattaagcat tctccttatg accttgaaga taaatggtca
     8401 aaccaaacaa aactgctgaa aaaacaagta tataaactgt ggctgataga ttgatgccac
     8461 cctcgtttaa gaaggttttg agcaactcca tcaactgata agttggtagg cacttcccga
     8521 ttgcttgcat ccagtctgga aataaagaga tgggcatcca gagtccgcct aaaacagcca
     8581 agcctagata gagaagattg cccacgacag acatcaactg actagaaggc aagagtgtca
     8641 aggtcaagcc aagcgctaca aaggcaacac ttcctactat tagcaagaac gcagccccaa
     8701 tccagcttcc tagaggcata tccacacctc ttacaaaatg cccaactgag aaaacaacca
     8761 gaattgatac caaataatca accatcatac ttgttatctt tgatagataa tattctacca
     8821 tatttacagg ggtatggcgc aatgttttct gccagttgtt gattttatca gtatgtaaga
     8881 cagctgggaa tgaaaacata gctgtcgaca tcatggaaaa ggctgtcatg gagatgaggt
     8941 aatcacgcat aaaattcgcg agttcacctg gtgtgtcctg atagatacca gaaaaaaata
     9001 aatagaaagc cgtcggcatc cctaccgaca atagataata gattaattgt cgtttggtca
     9061 ataaaaattc tatcttacta agtgctatcc atcgtttcat cttagttatc tcccttttgt
     9121 gtttcttcaa agattgtatt caacaaacta cgattattaa cttcgatttc ttgcatccta
     9181 catcctgctt gggctaatag ttcccagaaa gcatctgctt ctcgagtgac tacttgtaga
     9241 gcatcctgtt tttgtgacca gttttcaacc aagttagact gctcaatgac ttccttgtat
     9301 gccagaggaa ggataaaatg cttttcaatt ccctcactac gcatagctag aggcgtcgta
     9361 tcacgaatca actctccctt atttaaaacc aaaatccggt cagccgtatg ctctacctct
     9421 tcaatataat gagacgaata gagaatcgtg actccttgcg cttttaggtc ccgaacgatt
     9481 tcccaaaagc gttgacgagt tgaagtatcc atggcagcag ttggttcatc taaaaagaca
     9541 agctttggtc gcccaatcaa ggtcaagaca aaagagaaga gacgcttttg cccgcctgac
     9601 aatttttctg cgaattgctc tttttgttgc tggtcaaact gcaatagttg atcgatttcc
     9661 tgatcgctca aggaatttgg atagatacgt tgaaagaaag caatcaactc tttgaccttt
     9721 aatttctgaa cgatgacatt ttcttgaggc agataacctc taatatagtc taactgagaa
     9781 ctcgtcactg acaagccttg gatggatact tgaccgcttg tgaccagttt atctccaagc
     9841 agacagtcca agagtgtggt cttcccagca ccattgggcc caatcaaggc gacgcattca
     9901 ccttcagcta cctcaaagga aatacccttc aaaatagcct tgcccttgat gtttttattt
     9961 aggctttcta ccttaatcat attcatgata ttctcctttc aaccactcca ttctcataag
    10021 gaaaacgacg aaaatcataa atccaaaccc caaagcacca cgaatgaatt ggcgaagcaa
    10081 ggtttggtca aaccaacctg taaacatttc cactaaccat accaagagtg acaggccgat
    10141 aaagaaatag atgatccctc tcttcattcc tcaaactcct ttttcacatc tccgactaat
    10201 ttcaaacctt ctctaacaag ccaagacatc attccaaagc cagcaaagag ctcccaagga
    10261 aaatgataga aactctcatc caatcccgaa aacatgagtt aggtcataac tcctgctact
    10321 actaaactca ctgcgataat cattttattt ctcatctctt cttcctccat ttcatactac
    10381 aattatagtc ttttgaaatc agaggagaca gaagcttctg tcactagaaa atatgacaaa
    10441 tgtcataaaa aattctgttc aaaacaagca agatacacta tacaataaaa cacaatcaga
    10501 aaaatctaag gcaacttcct caaaagagat atcaaaccca attcacacca taatgtaaac
    10561 taatacttat ttaaaatcaa aaagagtaga aatttttatc agacaaacac at
//
LOCUS       DAVKFH010000068        10553 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKFH010000068 DAVKFH010000000
VERSION     DAVKFH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.068"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..1745
                     /locus_tag="VW006_001790"
     CDS             <1..1745
                     /locus_tag="VW006_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9380601.1"
                     /translation="YKTNNLFDALEKYRKAFVPDKTNNEWFKEQTKAYIVEEKSTIKE
                     VSDKQSIAGSPYSIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDR
                     YRSKEHPAGTDLNDYVEKKAKEAAVRFRDHYDYWYRILDDKNKEKLYRSVLVYDAFRF
                     GTDEKEDKDTYQATFETNHPAIKHFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKD
                     GAVTYTHEMTHNSDREIYLGGYGRRNGLGPEFYAKGLLQAPDHPNDPTVTINSILKYD
                     QSEESTRLQVADPTQRFGSVDDLNKYMHNMFDVIYMLEILEGKAVAKLDTNQKYDLLR
                     KIENEYKPDPDGNSVYATNVVRRLKPEELTKLTTFNSLIEHDIITRRGYVDEATYKRN
                     GYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMVPYISNQYEKEAKA
                     QGKVITSYGKQIGLVTDEIVLSKVFNNQYNSWIDFKKDMYKEREDKFGKLNKVSFIDP
                     NGSWARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRVLKLKKAIFKAYLD
                     QTGDFRSSIFENKK"
     gene            1789..7002
                     /locus_tag="VW006_001791"
     CDS             1789..7002
                     /locus_tag="VW006_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9380602.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGGNLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            7256..8107
                     /gene="ylqF"
                     /locus_tag="VW006_001792"
     CDS             7256..8107
                     /gene="ylqF"
                     /locus_tag="VW006_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9380603.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            8094..8873
                     /locus_tag="VW006_001793"
     CDS             8094..8873
                     /locus_tag="VW006_001793"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9380604.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8889..10439
                     /locus_tag="VW006_001794"
     CDS             8889..10439
                     /locus_tag="VW006_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9380605.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3612 a         1732 c         2154 g         3055 t
ORIGIN      
        1 attataagac taataattta tttgatgcat tagagaaata tagaaaagca tttgtaccag
       61 ataaaacaaa taatgagtgg tttaaagaac aaactaaagc atatattgta gaagaaaaat
      121 ctactattaa agaggtaagt gataagcaat caatagctgg tagtccatat tcaattggag
      181 tatatgatag attaactagc ccatcttgga aatatccaag tatggtgttg ccgctattaa
      241 cattacctga aaaatcggtg tttattatag ccaacatttc gactataggt ttcggtgctt
      301 atgatagata tagaagtaaa gaacatccag caggaactga tttaaatgac tatgttgaga
      361 aaaaagctaa agaagcggct gttagattta gagatcacta tgattattgg tatagaatac
      421 tagatgataa aaataaagaa aaattatata gaagtgttct agtctatgat gcatttagat
      481 ttggtacaga tgaaaaagaa gataaagata catatcaagc aacttttgaa actaatcatc
      541 cagctataaa acatttcttc ggtccagcgg gaaataatgt tgttcataat tctaatggag
      601 catatgctac aggggatgca ttctattata tggcatatcg catgcttgat aaagatggtg
      661 cggtaactta tacacacgaa atgacacata actcagatag agaaatttat cttggaggct
      721 atggaagacg aaatggactt ggaccagagt tttatgctaa aggactgcta caagctcctg
      781 atcatcctaa tgatccaaca gtcactatta attccatatt aaaatatgat caatcagaag
      841 aatcaacaag gcttcaagta gcagatccaa ctcaacgatt tggaagtgtt gatgatctaa
      901 ataagtatat gcataatatg tttgatgtaa tatacatgct tgaaatacta gaaggtaaag
      961 ctgtagctaa attagataca aatcaaaaat atgatttatt aagaaaaata gaaaatgaat
     1021 ataaacctga tcctgatgga aattctgttt atgcaacgaa tgttgtacgt agattaaaac
     1081 cagaagaact tactaaatta actacattta acagtttgat agaacatgat attataacaa
     1141 gaagaggtta tgtagatgaa gcaacttata agcggaatgg ctactataca attaatctat
     1201 tctcaccaat ttattcagca ttgagcagta aaataggaac accaggagat ttaatgggac
     1261 gacgtattgc atttgagtta ttggcagcca aggggtacaa agacggtatg gttccatata
     1321 tttctaatca gtatgaaaaa gaagcgaaag ctcaaggaaa agttattaca tcatatggta
     1381 aacaaatagg tttagttact gatgaaattg ttctttcaaa agtatttaat aaccagtata
     1441 attcttggat agatttcaag aaagatatgt ataaagaaag agaagataaa tttggaaagt
     1501 taaataaagt tagttttatc gatccaaacg gttcttgggc tcgacaacag aaagtgacta
     1561 ttgataatat taatagactt gaaaaaatga ttgaagatgc agtaaaattt gatgctgagg
     1621 atgaagttgc caaattatat cctgagacaa atagtagagt tcttaaactg aaaaaagcaa
     1681 tctttaaagc ttatcttgac caaaccggtg attttagaag ttcaattttt gagaataaaa
     1741 aatagtgttt actattagga aataaaaagt gaaggtggag gattagaatt gtcattatta
     1801 aaaaaagata aattttccat tcggaaaata aagggtattg ttggttcggt attcttgggg
     1861 agtcttttat ttgcaccgtc agttgttggt gcatcgactt atcattactt agattatagt
     1921 agtttgacac aaactgaacg tgatcaactc aaacaaggta gacctgatga atccaaagag
     1981 tcatatgctt tggattatga aaaagacgca ctaccaaaca caggaagttc tcaatctatt
     2041 atgactgcat tgggtttgtt agctatcggg agtcttattg tgattatcac aaaagataat
     2101 agaaataaaa aaatagctac atttttgatt gtaggggcga caggtttagt tacactatca
     2161 actgcttcag cacttaattt gaatgctaac atccatgagt ctggacgtga tggtgtgttg
     2221 caaatttctg gttacagata tgttggttac ttggagcttg atgataaaac tgtttcgtcg
     2281 gtttctccag catcaactgt ttcgccagtt gagcaaccca aagttgtaac agagaaaggc
     2341 gaaccggaag ttcatgagaa gcctgactat acgcaaccaa taggtggaaa ccttgttgag
     2401 cctgaagttc atgagaaact agcatacact gaaccagtag ggacaacagg agtagatgaa
     2461 aatggaaact tgattgaacc acctgttaac gacatcccag aatatactga gccagtaggt
     2521 acaacaggag tggatgaaaa tggaaacttg attgagccac ctgttagcga cattccagag
     2581 tatactgaac caatatctac agtttcagaa gttgcatcag aaagagaaga gttgccttct
     2641 ttacataccg atattcggac agagactatt cctaaaacca ctatagaaga atctgatcct
     2701 agcaaattta taggcgatga ttctgttaga caagttggtg aggatggcga acgtcaaatt
     2761 gttactagct atgaagagtt acatggcaaa aaaatcagtg acccagttga aacagtaact
     2821 attttgaaag aaatgaagcc taaaattctt gtaaaaggta ccaaagaaaa acctaaagaa
     2881 aaaacggctc ctgttttgac cttagataga acaaatacaa atgtattaaa ccgatccgca
     2941 actctttctt atcatttagt taatacagat ggagtcacaa tcaataaaat tactgcgaca
     3001 attaaagatg gaaatgaaat tgtcaaaaca gtagatttga cttcagagca attagataag
     3061 caggtagaag atttaaaatt ctacaaggac tataagatag agaccactat gacctatgac
     3121 cgtggcaagg gagaagaaac tgcaacatta gaagagaaac cacttcgctt ggatttgaaa
     3181 aaagttgaaa ttaaaaatat cgcatctact aatttagtga aggtaaatga cgatggtact
     3241 gaaacaccta gtgattttat gactgaaaaa ccttcagatg aagatgtgaa gaaaatgtac
     3301 ttaaaaatta ctagtcgtga taataaagta acacgtttag cagttgataa aattgaagaa
     3361 gtgactgaag aaggtaagaa actttataaa atcactgccg aagctcaaga tttgattcaa
     3421 catacagacc caacgaaggt tcgtaacaag tatgttcatt atattgaaaa accagttcca
     3481 aaagttgatg acgtttatta taactttaaa gagttagtag atgccatgaa tgctgataaa
     3541 aatggtacat ttaaaatcgg agcagatttg aatgcaacga atgtaccaac acctaacaaa
     3601 caatatgttc caggtacgtt taagggacat ctatcaagtg ttgatggtaa acaatataca
     3661 attcataaca ttgcccgtcc cttatttgac cgtgttgaaa atggttctgt gaaaaatatt
     3721 aatttaggta atgtggatat caatatgcct tgggctgacg gaatagcacc tgttgccaat
     3781 atggtgaaaa atgcaactgt tgaagatgtt aaggtaactg gtaatgtagt tgctaataac
     3841 aatattgcgg gtatcgtcaa taaaatcgat agtggtggtc agttgacaaa tgtagccttc
     3901 atcggtaatc ttacaggtgt tggtgataaa ggtcaatata tggctggcat tgccggtgag
     3961 atttggagag gtaatcttgc aaaagcatat gtcgaggctg atattgttgc taacagagcc
     4021 cgtataggtg gtttagttgc taaaacagat aatggtaacg actctatggg aattggtaaa
     4081 tacggttcaa ttcgtaaatc agtcaccaaa ggaaccatta agactaaagt tttgtttgaa
     4141 acaggtggat ttatcaatag caatcttcct tttggtaaac ttgaagataa tatttcaatg
     4201 atgagagttg aaaatggtga agagttcttt ggttcttccg atttggacta cgatggtggt
     4261 tatttcacaa atggctggtt agagcgtaac tttgttgtca aaggtgtcag ttcaggtaag
     4321 cattcataca aacgttctcg tgacaaaatt aaagaaatta gtcaagatga agctaataag
     4381 agaattgcaa attttggact tactgctgat aaatacgaaa tcaacgaacc tgtggtcaat
     4441 cgcttaaatc gtttaaccag aagagaagat gaatataagt caactcaaga ctacaaatcc
     4501 gaacgtgatt tagcttatcg taatattgag aaacttcaac cattttataa taaagaatgg
     4561 attgtcaacc aaggtaacaa acttgcagaa gattcaaact tagctaagaa agaagtcctt
     4621 tctgttacgg ggatgaagga tggtcaattc gtgaccgatt tatcagatat tgataagatt
     4681 atggttcact atgctgatgg tactaaggaa gaaatggatg ttactaagaa tactgattct
     4741 aaagtccaac aagtgcgtga atatagtgta tctggtcttg gtgatgttgt ttatacaccg
     4801 aatatggttg ttaaaaatcg tgataaattg attgctgatg ttaaatctca attatcaagt
     4861 gtggaattga tttcacaaga agtaagagat ttaatgagta gacgtgataa acctgcagag
     4921 aatacggatg aacgtaagaa tggttatatt aaagatctat acttagaaga aagctttgcc
     4981 gaagtgaagc aaaatctaga taagttagtc aaatccttgg tcgaaaatga agaccatcaa
     5041 ttgaatggtg atgaagctgc gataaaatca cttctcaaga aagtggaaac caataaagct
     5101 aaaatcatga tggctctgac ttatctcaat cgttactatg atatcaaata cggtgacata
     5161 agtataaaaa acatcatgat gttcaaacct gatttttatg gtaaaacacc aagtgttatt
     5221 gacagactca tcaacatcgg ttcaagtgaa aagaacttga aaggggatcg gactcaggat
     5281 gcttaccgtg aaatcatcgc aggtaatact ggtaaaagta atttgcgtaa cttcttagaa
     5341 tacaacatgc gtctgtttac agaggataaa gacatcaatg attggtttat ccactcagct
     5401 aagaatgttt atgtttcaga acctaagaca acaaatacag agttgaaaga taaacgtcat
     5461 cgtgtgttcg atggtttaga taacggtgtt catggtcgta tgattttacc acttctaacg
     5521 cttaaagatg cccatatgtt cttaatttca acatataaca cgatggcgta cagttcattt
     5581 gagaaatatg gaaaacatac agaagaagct cgtaatgagt tcaaaactaa gattgatgaa
     5641 gttgcacatg ctcaacaaac ttatttagat ttctggtcca gattagctct gcctaatgtt
     5701 cgtgatagac ttcttaagag tcaaaatatg gtaccgacac cagtttggga taaccagacc
     5761 tacaatggtt ctcctgtagg tcgtcgagga tttgacagta aaggtaaccc tatagctcca
     5821 attcgtgaat tatatggtcc aacatggcga caccatgata gagactggcg tatgggcgct
     5881 atggcatcaa tattccctaa tccaaataat gatgacaagg tcttgtttat ggtgacggat
     5941 atgattagcc catttggtat ttcagccttt actcacgaaa caacacacgt aaacgataga
     6001 atgctttact tcggtggtca taagcatcgt caaggtacag acgttgaagc ctatgctcaa
     6061 ggtatgttgc aaacacctga cagctcaacg actaatggtg aatacggcgc acttggaatt
     6121 aacatggctt accatcgtcc aaatgatggt aaccaatggt acaacccaga tcctgacaaa
     6181 ttaaagacac gtgatgacat tgaccgctac atgagaaact acaatgaagc tatgatgttg
     6241 cttgatcatg tagaagctga tgctgtactt cctaaaatta aaggagacaa tagtaagtgg
     6301 ttcaagaaga ttgacaaaga aatgcgctct aagattcagt ataatgatct tttaggtcca
     6361 aaccaatggg atagtatccg tgatttgaaa gatgaagaaa aggtaatgac tttatctagc
     6421 gtcaatgatt tagtggataa taacttcatg acaaaacatg gaaatccagg taatggacgt
     6481 tatagaccag aagattttac accaaactca gcttacgtaa atgtcaatat gatggcagga
     6541 atttacggtg ggaatacgag tcaaggtgct cctggttcat tatcatttaa gcacaatgct
     6601 ttccgtatgt ggggctatta tggttatgaa aatggcttta ttagttatgt atcaaataaa
     6661 tacaaagcag aagcagataa aaacaaccac ggtttactaa gcgataagct aataattaat
     6721 aaagtatcga aaggaaactt taatactctt gaagagtgga agagacattg gtacggggaa
     6781 gttcttgcta aagctaagaa aggttttgag gctatcgata ttgatggcgt ccatatcagt
     6841 aactatgatg agttaaggcc tttatttgat aaagcagttg aagaagattt gaagaaacca
     6901 gatgacttct ctcacactgt tgctcttaaa tctaaggtct tcaaagccct tcttaaaaac
     6961 acagacggtt tctttaataa actgtttaaa gaagacattt aaatgtttag aatatagaag
     7021 gaacagtttt ttgctccctc tgaaaagtca tcatttgatg gcttttttct atatagggta
     7081 cttgaaaggc tatcatcgga caaaataaag aaggcatgat ataatataag gtagatttct
     7141 atgccataaa acaaaaaact gtttgaacat cattcatttg agaacccttt atgttcaaac
     7201 gatagtaaaa taaaatagga tctctaaacc cttgctacga aaggaaaaaa actcaatggc
     7261 tactattcaa tggtttcctg gtcacatgtc taaagcgcgt cgacaggtgc aggaaaattt
     7321 aaaatttgtt gattttgtaa cgattttggt ggatgcacgc ttacctctat ctagtcaaaa
     7381 tcctatgttg accaagattg ttggtgataa accaaaactc ttgattttaa ataaggcgga
     7441 tttggctgat ccagcaatga ccaaggaatg gcgtcagtat tttgaatcac aaggaatcca
     7501 aactctggct atcaactcca aggagcaagt gactgtaaaa gttgtaacag atgcggccaa
     7561 gaagctcatg gctgataaga ttgctcgcca gaaagaacgt gggattcaga ttgaaacctt
     7621 gcgtactatg attatcggga ttccaaacgc tggtaaatca actctgatga accgtttggc
     7681 tggtaaaaag attgctgttg ttggaaacaa gccaggggtc acaaaaggtc aacaatggct
     7741 taaaaccaat aaagacctgg aaatcttgga tacaccgggg attctctggc ctaagtttga
     7801 ggatgaaact gttgcactta agttggcatt gactggagct atcaaggatc agttgcttcc
     7861 tatggatgag gttaccattt ttggtatcaa ttatttcaaa gaacattatc cagaaaagct
     7921 ggctgaacgc ttcaaacaaa tgaaaattga agaagaagcg cctgtgatta ttatggatat
     7981 gacccgcgcc ctcggtttcc gtgatgacta tgaccgtttt tacagtctct tcgtgaagga
     8041 agtccgtgat ggcaaactcg gtaactatac cttagataca ttggaagacc tcgatggcaa
     8101 cgattaaaga aatcaaagaa ttccttgtga cagtcaagga gttagaaagc cctatttttt
     8161 tagagcttga aaaggataat cgctcaggag ttcaaaagga aatcagcaag cgtaaaagag
     8221 ccattcaagc tgaattagat gaaaatttgc gcttggaatc catgctttct tatgaaaaag
     8281 aactttataa gcaaggattg accttaattg taggtattga tgaggttggt cgtggtcctc
     8341 ttgctggtcc tgtagtcgct gcggccgtca ttttacctaa aaattgtaag attaaaggtc
     8401 tcaacgacag caagaaaatt cctaaaaaga aacatctgga gattttccaa gccgttcaag
     8461 accaagcctt gtcgattgga attggtatca tagataatca ggtcatcgac caagtcaaca
     8521 tctatgaagc aaccaaacta gccatgcaag aagcaatctc ccagctcagc cctcaaccag
     8581 agcacctttt gattgatgcc atgaaactgg acttgcccat ttcacaaacc tccattatca
     8641 aaggagatgc caactccctc tctatcgcag cagcatctat agtagccaag gtaacacgtg
     8701 atgaattgat gaaggaatac gatcagcagt tccctggcta tgatttcgct actaatgcag
     8761 gatatggcac agctaaacat ctggaaggcc tcacaaaact aggagttacc ccaattcacc
     8821 gaaccagctt tgaacccgtt aaatcactgg ttttaggtaa aaaagaaagt taattgaaag
     8881 gaaataccat ggaggaacag tcggaaatag tccgttctaa gaaagaattc gcctttgcat
     8941 ccagcactat actatcccaa gttggtcgag gaatcattgt cggcctcatc gttggaatta
     9001 tcgtcggatc ctttcgtttc ttaattgaaa agggcttcca cctgatacaa ggagtttatc
     9061 aagatcaagg gtacttagtg cgcaatcttt ttgtactggt tttgttttat atactcatct
     9121 gttggctcag tgccaaacta acacggtcag aaaaagatat taaaggctca ggaattcctc
     9181 aagtcgaagc cgaactgaaa ggcctcatgt ccctcaactg gtggggcatt ctttggaaaa
     9241 aatatgtgct aggtattctt gctattgcca gtggactcat gctgggtcga gagggaccca
     9301 gtattcaact tggagcagtt ggtggtaaag gaattgccaa gtggctcaaa tccagtccag
     9361 tagaggaacg ttccttgatt gccagtggag ctgcagcagg tttagccgca gcctttaatg
     9421 ctcctattgc agcacttctc tttgttgtag aagaagtcta tcaccatttt tcgcgctttt
     9481 tctgggtctc aactctagca gccagcatcg tagcaaactt tgtgtctcta ctcatgttcg
     9541 gtttgacacc agtattggat atgccagata acattcctcc catgacccta gatcagtatt
     9601 ggatatatct cgtcatggga attttccttg gattttcagg ttttctctat gagaaagctg
     9661 tattaaacgt tggaagagtt tatgacttga ttggtcaaaa aatccatttg gatagggctt
     9721 attatcccat cttggctttt atccttatca taccagtcgg aatcttctta cctcaaatca
     9781 ttggtggcgg aaatcagctt gtcctttctt taactgaaca aaattttagt ttccaagttt
     9841 tattagctta ctttttaatc cgctttattt ggagtatgat tagctatgga agtggactgc
     9901 caggaggaat tttcctcccc attttagctc ttggttcttt gcttggtgcc ttagttggtg
     9961 ttatctgtgt caatcttgga cttgtcagtc aagagcaatt ccctatattt gtcattctag
    10021 gaatgagtgg ctattttgga gccatatcaa aagctccctt aaccgctatg atcctcgtaa
    10081 ctgagatggt aggagatatt cgcaacctta tgccacttgg tcttgtcact cttgtttctt
    10141 atattatcat ggatttgctc aaaggtacgc cagtctatga agccatgctg gaaaaaatgc
    10201 ttccagaaga agtatctagc gaaggagaag ttacacttat cgaaatacca gtttctgata
    10261 aaattgctgg gaaacaagtt catgaactca acttaccaca caacgtcctc atcacaactc
    10321 aagtccataa tggcaagagc caaacagtta acggctcaac cagaatgtat ctgggtgata
    10381 tgattcacct ggttattcca aaaagtgaaa ttggaaaagt caaagatttg ttgttgtagt
    10441 atgatcattt acataattta tgttatgtaa atgatcagtt tgatttattt agaaaaccga
    10501 ttctcagaaa tgagatcggt tattttttac tgatgaggaa ttttacatat aaa
//
LOCUS       DAVKFH010000069        10305 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKFH010000069 DAVKFH010000000
VERSION     DAVKFH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.069"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            132..5042
                     /locus_tag="VW006_001795"
     CDS             132..5042
                     /locus_tag="VW006_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9380606.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYRFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            5132..6328
                     /locus_tag="VW006_001796"
     CDS             5132..6328
                     /locus_tag="VW006_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9380607.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     VALTEK"
     gene            6464..6991
                     /locus_tag="VW006_001797"
     CDS             6464..6991
                     /locus_tag="VW006_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9380608.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7068..7424
                     /gene="glnR"
                     /locus_tag="VW006_001798"
     CDS             7068..7424
                     /gene="glnR"
                     /locus_tag="VW006_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9380609.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7461..8807
                     /gene="glnA"
                     /locus_tag="VW006_001799"
     CDS             7461..8807
                     /gene="glnA"
                     /locus_tag="VW006_001799"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9380610.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8903..9022)
                     /locus_tag="VW006_001800"
     CDS             complement(8903..9022)
                     /locus_tag="VW006_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9380611.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9510..10305
                     /locus_tag="VW006_001801"
                     /pseudo
     CDS             9510..10305
                     /locus_tag="VW006_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3356 a         1842 c         2226 g         2881 t
ORIGIN      
        1 agttattgtt ttttgtctaa aaatgtttta ggataaaaat gtgattatca tggttctaga
       61 gtttgataaa aaactacaga atataaagcg ctttctttaa aacaacaaaa tcaatctttt
      121 aggaggagaa aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt
      181 tatcagtagg agcctgctcg ctgatgattg gtgctgtttt atttgctggt ccagccttgg
      241 ctgaagaaac tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga
      301 gtgagcattc gaccaatgaa gctgataagc agaatgaagg ggaacatgct agagaaaaca
      361 agctagaaaa ggcagaagca gctagcgcag ctaaaccaga ggaaaaagca ggtgaggtgg
      421 ttgcagaaac accatctgca gaagcaaaac ctaagtctga caaggaaaca gaagcaaagc
      481 ccgaagcaac taaccaaggg gatgagtcta agccagcagc agaagctaat aagactgaaa
      541 aagaagtcca gccagatgtc cctaaaaata cagaaaaaac attaaaacca aaggaaatca
      601 aatttaattc ttgggaagaa ttgttaaaat gggaaccagg tgctcgtgaa gatgatgcta
      661 ttaaccgcgg atctgttgtc ctcgcttcac gtcggacagg tcatttagtc aatgaaaaag
      721 ctagcaagga agcaaaagtt caagccttat caaacaccaa ttctaaagca aaagaccatg
      781 cttctgttgg tggagaagag ttcaaggcct atgcttttga ctattggcaa tatctagatt
      841 caatggtctt ctgggaaggt ctcgtaccaa ctcctgacgt tattgatgca ggtcaccgta
      901 acggggttcc tgtatacggt acactcttct tcaactggtc taatagtatt gcagatcaag
      961 aaagatttgc tgaagctttg aagcaagacg cagatggtag cttcccaatt gcccgtaaat
     1021 tggtagacat ggccaagtat tatggctatg atggctattt catcaaccaa gaaacaactg
     1081 gagatttggt taaacctctt ggagaaaaga tgcgccagtt tatgctctat agcaaggaat
     1141 atgctgctaa ggtaaaccat ccaatcaagt attcttggta tgatgccatg acctataact
     1201 atggacgtta ccatcaagat ggtttgggag aatacaacta ccaattcatg caaccagaag
     1261 gagataaggt tccggcagat aacttctttg ctaactttaa ctgggataag gctaaaaatg
     1321 attacactat tgcaactgcc aactggattg gtcgtaatcc ttatgatgta tttgcaggtt
     1381 tggaattgca acagggtggt tcctacaaga caaaggttaa gtggaatgac attttagacg
     1441 aaaatgggaa attgcgcctt tctcttggtt tatttgcccc agataccatt acaagtttag
     1501 gaaaaactgg tgaagattat cataaaaatg aagatatctt ctttacaggt tatcaaggag
     1561 accctactgg tcaaaaacca ggtgacaaag attggtatgg tattgctaac ctagttgcgg
     1621 accgtacgcc agcggtaggt aatactttta ctacttcttt taatacaggt catggtaaaa
     1681 aatggttcgt agatggtaag gtttctaagg attctgagtg gaattatcgt tcagtatcag
     1741 gtgttcttcc aacatggcgc tggtggcaga cttcaacagg ggaaaaactt cgtgcagaat
     1801 atgattttac agatgcctat aatggcggaa attcccttaa attctctggt gatgtagccg
     1861 gtaagacaga tcaggatgtg agactttatt ctactaagtt agaagtaact gagaagacca
     1921 aacttcgtgt tgcccacaag ggaggaaaag gttctaaagt ttatatggca ttctctacaa
     1981 ctccagacta caaattcgat gatgcagatg catggaaaga gctaaccctt tctgacaact
     2041 ggacaaatga agaatttgat ctcagctcac tagcaggtaa aaccatctat gcagtcaaac
     2101 tatttttcga gcatgaaggt gctgtaaaag attatcggtt caacctagga caattaacta
     2161 tctcggacaa tcaccaagag ccacaatcgc cgacaagctt ttctgtagtg aaacaatctc
     2221 ttaaaaatgc ccaagaagcg gaagcagttg tgcaatttaa aggcaacaag gatgcagatt
     2281 tctatgaagt ttatgaaaaa gatggagaca gctggaaatt actaactggc tcatcttcta
     2341 caactattta tctaccaaaa gttagccgct cagcaagtgc tcagggtaca actcaagaac
     2401 tgaaggttgt agcagtcggt aaaaatggag ttcgttcaga agctgcaacc acaacctttg
     2461 agtgggggat gactgtccaa gacacaagtc ttccaagacc tctagcagaa aacatcgttc
     2521 caggggctac tgttatcgga agtaccttcc cagatacaga aggtggagaa ggtattgaag
     2581 gtatgttgaa cggtaccatt actagcttgt cagataaatg gtcttcagct cagttgagtg
     2641 gtagtgtgga tattcgtttg accaagccac gtaccgttgt tagatgggtc atggatcatg
     2701 caggggctgg tggtgagtct gttaacgatg gcttgatgaa caccaaagac tttgaccttt
     2761 attataaaga tgcagatggt gagtggaagc tagctaagga agtccgtggc aacaaagcac
     2821 acgtgacaga tatcactctt gataaaccaa tcactgctca agactggcgc ttgaatgttg
     2881 tcacttctga caatggaact ccatggaagg ctattcgtat ctataactgg aaaatgtatg
     2941 aaaagcttga tactgagagt gtcaatattc cgatggccaa ggctgcagcc cgttctctag
     3001 gcaataacaa ggtacaagtt ggctttgcag atgtaccggc tggagcaact attaccgttt
     3061 atgataatcc aaattctcaa actccactcg caaccttgaa gagcgaagtt ggaggagacc
     3121 tagcaagtgc accattggat ttgacaaatc aatctggtct tctttattat cgtacccagt
     3181 tgccaggcaa ggaaattagt aatgtcctag cagtttccgt tccaaaagat gacagaagaa
     3241 tcaagtcagt cagcctagaa acaggaccta agaaaacaag ctacgccgaa ggggaggatt
     3301 tggaccttag aggtggtgtt cttcgagttc agtatgaagg aggaactgag gacgaactca
     3361 ttcgcctaac tcacgcaggt gtatcagtat caggttttga tacgcatcat aagggagaac
     3421 agaatcttac tctccaatat ttgggacaac cagtaaatgc taatttgtca gtgactgtca
     3481 ctggccaaga cgaagcaagt ccgaaaacta ttttgggaat tgaagtaagt caggaaccga
     3541 aaaaagatta cctagttggt gatagcttag acttgtctga aggacgcttt gcagtggctt
     3601 atagcaatga caccatggaa gaacattcct ttactgatga gggagttgaa atttctggtt
     3661 acgatgctca aaagactggt cgtcaaacct tgacgcttcg ttaccaaggt catgaagtca
     3721 actttgatgt tttggtatct ccaaaagcag cattgaacga tgagtacctc aaacaaaaat
     3781 tagcagaagt tgaagctgct aagaacaagg tggtctataa ctttgcttca ccagaagtaa
     3841 aagaagcttt cttgaaagca attgaagcgg ccgaacaagt gttgaaagac catgaaacta
     3901 gcacccaaga tcaagtcaat gaccgactta ataaattgac agaagctcat aaagctctga
     3961 atggtcaaga gaaatttaag gaagaaaaga cagagcttga tcgtttaaca ggtgaggttc
     4021 aagaactctt ggctgccaaa ccaaaccatc cttcaggttc tgccctagct ccgcttcttg
     4081 agaaaaacaa ggccttggtt gaaaaagtag atttgagtcc agaagagctt gcaacagcga
     4141 aacagagtct aaaagatctg gttgctttat tgaaagaaga caagccagca gtcttttctg
     4201 atagtaaaac aggtgttgaa gtacacttct caaataaaga gaagactgtc atcaagggtt
     4261 tgaaagtaga gcgtgttcaa gcaagtgctg aagagaagaa atactttgct ggagaagatg
     4321 ctcatgtctt tgaaatagaa ggtttggatg aaaaaggtca agatgttgat ctctcttatg
     4381 cttctattgt gaaaatccca attgaaaaag ataagaaagt taagaaagta tttttcttac
     4441 ctgaaggcaa agaggcagta gaattggctt ttgaacaaac ggatagtcat gttatcttta
     4501 cagcacctca ctttactcat tatgcctttg tttatgaatc tgctgaaaaa ccacaacctg
     4561 ctaaaccagc accacaaaac acagtccttc caaaacctac ttatcaaccg gcttctgatc
     4621 aacaaaaggc tcctaaattg gaagttcaag aggaaaaggt tgcctttcat cgtcaagagc
     4681 atgaaaatgc tgagatgcta gttggggaac aacgagtcat catacaggga cgagatggac
     4741 tgttaagaca tgtctttgaa gttgatgaaa acggtcagcg tcgtcttcgt tcaacagaag
     4801 tcatccaaga agcgattcca gaaattgttg aaattggaac aaaagtaaaa acagtaccag
     4861 cagtagtagc tacacaggaa aaaccagctc aaaatacagc agttaaatca gaagaagcaa
     4921 gcaaacaatt gccaaataca ggaacagctg atgctaatga agccctaata gcaggcttag
     4981 ccagccttgg tcttgctagt ttagccttga ccttgagacg gaaaagagaa gataaagatt
     5041 aaatatcgaa aaatcttgtg aaatctttcc ttatatttct aaagtgtgat ataatagttt
     5101 cgaataaaat aaataaagga gtttttgtaa catggcaaaa cttactgtta aagacgttga
     5161 cttgaaaggt aaaaaagtcc tcgttcgtgt tgacttcaac gtaccattga aagatggtgt
     5221 aatcactaac gataaccgta tcacagcagc tcttccaact attaagtaca tcatcgaaca
     5281 aggtggacgt gcaattcttt tctctcacct tggacgtgtg aaagaagaag ctgataaagc
     5341 tggtaaatca cttgctcctg tagcagctga cttggcagca aaacttggtc aagatgttgt
     5401 tttcccaggt gtcactcgtg gtgctgaatt ggaagcggca atcaacgctc ttgaagatgg
     5461 acaagttctc ttggttgaaa acactcgtta cgaagatgtt gacggcaaga aagaatctaa
     5521 aaacgatcct gaacttggta aatactgggc atcacttgga gatggtatct tcgtaaacga
     5581 tgcattcggt acagctcacc gtgcacacgc atctaacgtt ggtatctcag caaacgttga
     5641 aaaagcagtt gctggtttcc ttcttgaaaa cgaaattgcc tacatccaag aagcagttga
     5701 aactccagaa cgtccattcg tggctatcct tggtggttca aaagtttcag acaagatcgg
     5761 tgttatcgaa aacttgcttg aaaaagctga taaagtcctt atcggtggtg ggatgactta
     5821 cacattctac aaagcacaag gtatcgaaat cggtaactca cttgtagaag aagacaaatt
     5881 ggatgttgcg aaagctcttc ttgaaaaagc aaatggtaaa ttgatcttgc cagttgactc
     5941 aaaagaagct aacgcatttg ctggttacac tgaagtgcgt gacactgaag gtgaagcagt
     6001 ttctgaaggc ttccttggtc ttgacatcgg tccaaaatct atcgccaaat ttgacgaagc
     6061 tttgactggt gccaaaacag ttgtatggaa cggacctatg ggtgtatttg aaaacccaga
     6121 tttccaagct ggtacaatcg gtgtgatgga cgctatcgtg aaacaaccag gagttaaatc
     6181 aatcatcggt ggtggtgact cagctgccgc agcgattaac cttggccgtg cagacaagtt
     6241 ctcatggatt agtacgggtg gtggagcatc aatggaactt cttgaaggta aggttcttcc
     6301 aggacttgta gccttgacag aaaaataaga ttttataaac aaatcaaaga agagagggat
     6361 gaaagttcct cttttctttt gcttaaaata aaaacgcttc ctctcaacta ttactcataa
     6421 aaatcaccga tttatgataa aatggaaata gaaagttgag attatgagtt attttaaaaa
     6481 atataaattc gataaatccc agttcaaact tggtatgcga acctttaaaa caggtattgc
     6541 tgtttttcta gttctcttga tttttggctt ttttggctgg aaaggtcttc aaattggtgc
     6601 tttgacagcc gtttttagcc tgagggagag ttttgatgag agtgttcatt ttgggacttc
     6661 gcgtattcta ggaaatagta tcggtggact ctatgccttg gtcttcttct tattaaatac
     6721 ctttttccac gaagcctttt gggtgacctt ggtagttgtt ccaatctgca ccatgttaac
     6781 cattatgaca aatgtagcca tgaataacaa agcaggggtt attggtggtg tagcagctat
     6841 gttaattatt accctatcaa ttccaagtgg tgagacaatt ttgtacgtgt ttgtgcgtgt
     6901 attagaaaca tttatgggag tttttgtcgc aattatcgta aattacgata ttgatcgtat
     6961 tcgtctcttt ttagagaaaa aagaaaaata atgttacatt ttataacatt attaattgac
     7021 gtttgtcttt ttttagacta taatagacag aaagaaggaa attgtaaatg aaggaaaaag
     7081 aatttcgccg aaatatggct gtttttccta tcggcagtgt tatgaagttg accgatctat
     7141 cggcgcgtca gattcgttat tatgaaaatc aagagttgat caagcccgat cgaaacgaag
     7201 gaaatcgtcg catgtattcc ttgaatgaca tggatcgtct gcttgaaatc aaagattata
     7261 tctctgaagg ttataatatc gctgccatta agaaaaaata tgctgaacgt gaagcgaaat
     7321 ccaagaaagc ggttagtcag actgaagtac gtcgtgcact tcacaatgaa ctcctccaac
     7381 aggggcgttt tgcttcagta cagtcacctt ttggtcgcgg ttaggcaacc gcaagtagtc
     7441 atagattaag gagaaaactt atgccaatca cagctgcaga tattcgtcgt gaagtcaagg
     7501 aaaaaaatgt tacctttatt cgtcttatgt tctcagatat tttgggaacc atgaaaaacg
     7561 tcgaaattcc tgctacagat gaacagttag ataaggtctt gtcgaacaag gttatgtttg
     7621 atggatcttc tattgaaggt tttgtacgta tcaatgagtc ggatatgtac ttgtacccgg
     7681 acttggatac atggacagtc ttcccttggg gagatgaaaa tggaagtgtt gcaggtctga
     7741 tctgtgatgt ttatacaaca gaaggtgaac catttgcggg tgaccctcgt ggtaatttga
     7801 aacgagctct tcgtcacatg gaagaagttg gattcaaatc cttcaacctt ggtccagagc
     7861 cagaattctt cctatttaag ttggatgaaa atggggaccc aacacttgaa gtgaatgaca
     7921 agggtggcta ctttgatttg gcacctactg accttgcgga caacacacgt cgtgagattg
     7981 tgaatgtctt gaccaaaatg ggatttgaag tagaagcgag tcaccacgag gttgcggttg
     8041 gacagcatga gattgacttt aagtacgatg aagttctccg tgcttgtgat aagattcaaa
     8101 tctttaaact tgttgttaaa accattgctc gcaaacacgg actttacgca acatttatgg
     8161 cgaagccaaa atttggtatt gctggctcag gtatgcactg taatatgtcc ttgtttgatg
     8221 cagaaggaaa taacgccttc tttgatccaa atgatccaaa aggaatgcag ttgtcagaaa
     8281 cagcttacca tttcctaggc ggtttgatca agcatgctta caactatact gccatcatga
     8341 acccaacagt taactcatac aaacgtttgg ttccaggtta tgaagcgcct gtttacattg
     8401 cttgggctgg tcgtaaccgt tcgccacttg tgcgcgtacc tgcttcacgt ggtatgggaa
     8461 ctcgtcttga gttgcgttca gtggatccaa tggcgaaccc ttacgttgct atggctgttc
     8521 ttttggaagt tggtttgtat ggtattgaaa ataaaatcga agcaccagct cctatcgaag
     8581 aaaatatcta catcatgaca gcagaagagc gcaaggaagc tggtattaca gaccttccat
     8641 caactcttca caacgctttg aaagctttga cagaagatga agtggttaaa gctgctctcg
     8701 gagatcacat ctatactagc ttccttgaag ccaaacgaat cgaatgggca agttatgcaa
     8761 ccttcgtttc acaatgggaa attgataatt atttagacct ttactaatac taatatagaa
     8821 gaaagattgc ctgtgggtaa acagtcacac agcagtctaa gccatcaaat acctataacc
     8881 cttaaaatag cgatttcggt cgctatttat ttttcgaaaa atcctcttga ctcttttgcg
     8941 aattagattt atcatcagac gtgtagaaga aaattaagga gtcactatgg aaaaattcaa
     9001 tttcaaaaac aatatcgggc aagaaaacaa gttacttcaa atcgaaatct acaagtttac
     9061 taacttttgc aagcttcaaa attacacatc tgtaaatatt ttttcgaaag atatatttga
     9121 agccatagta aattgagact agaatagtac actacatctg ctaaaaattt ctagaaacta
     9181 atttaacttt cctaatcgat ttgtttacat cttatttcaa tctactatat tatctcaata
     9241 aatgatgatg gcgactgttt tgtttgtgct tgtcgatttt tgaatcctga tcgagcggaa
     9301 gaatatttct gccgaggttg ccaaggggaa aatcctgaag atattgaatt ttatgatgaa
     9361 caattacagg cagaaaaggt ggaggttttg aatattcgat tggaagtaaa aaattgaatt
     9421 atctacaatc tcatcaacaa taaacctatt tcaaccccat tatctatagg cgtattttta
     9481 cgccttttta tatacttgaa gcacataaga aacattatgg gaagtttgct gatggaagca
     9541 ctcaagaaat tgatgttcct tatgatattc ctgatacttg ggagtgggtg aggttttcta
     9601 cattggttga aattgtcaga ggtggctctc cacgaccaat caaagattat cttacttctg
     9661 aagtagatgg aataaattgg ataaaaatag gtgatactga aaagggtgaa aagtatataa
     9721 ataatgttaa agaaaaaatc aaaaaatcag ggcttaacaa aactagattt gtaaaaaaag
     9781 gtacattttt gttaactaat tctatgagtt ttggtagacc ttatattttg aatgttgatg
     9841 gtgcaataca cgatggatgg ttggctattt cgaactatga aaactcatta aataaagatt
     9901 acctattcta tattctttca tcaaatgtag tttattctca atttctatct ctaattagtg
     9961 gagctgttgt gaaaaacttg aatagtgata aagttgcttc tattcttatc cctctccccc
    10021 cactatccga acaacaacgc ataatagaag caatcgaatc agctttagaa aaagtagatg
    10081 aatatgctga aagttataat agactagaac agctagataa agaatttcca gataaactaa
    10141 aaaaatctat tcttcaatat gctatgcaag gaaaattagt tgaacaagac ccaaatgatg
    10201 aatcagtcga agttttactt gaaaaaatac gagcagaaaa acaaaaactc tttgaagaag
    10261 gcaagattaa aaagaaagat ttggacattt ctattgtttc ccaag
//
LOCUS       DAVKFH010000070        10203 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKFH010000070 DAVKFH010000000
VERSION     DAVKFH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.070"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VW006_001802"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VW006_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9380612.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1317..2021
                     /locus_tag="VW006_001803"
     CDS             1317..2021
                     /locus_tag="VW006_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380613.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="VW006_001804"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="VW006_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9380614.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="VW006_001805"
     CDS             3365..4174
                     /locus_tag="VW006_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9380615.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /locus_tag="VW006_001806"
     CDS             4265..4507
                     /locus_tag="VW006_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9380616.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="VW006_001807"
     CDS             4509..4763
                     /locus_tag="VW006_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9380617.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4810..5505
                     /locus_tag="VW006_001808"
     CDS             4810..5505
                     /locus_tag="VW006_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU9380618.1"
                     /translation="MKKKWLFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(5854..6840)
                     /locus_tag="VW006_001809"
     CDS             complement(5854..6840)
                     /locus_tag="VW006_001809"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9380619.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7034..9502
                     /gene="gyrA"
                     /locus_tag="VW006_001810"
     CDS             7034..9502
                     /gene="gyrA"
                     /locus_tag="VW006_001810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9380620.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9502..>10203
                     /gene="srtA"
                     /locus_tag="VW006_001811"
     CDS             9502..>10203
                     /gene="srtA"
                     /locus_tag="VW006_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9380621.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDE"
BASE COUNT         3158 a         1736 c         2286 g         3023 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattc ctggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggcta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccggat cgtccgggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggccccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact attttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacgaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaagctct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg tgtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aaatcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaggtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 accaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cacaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtgctccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tccttttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggctatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgactcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaataa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact gaacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgttttat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaac caatgttttg gaaggtccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt cgttgctact ctagaagttg
     4741 gtcatcggag agatatttat tgaattaaag ctgagtaata acaatataga aagtaaggag
     4801 agtaaaaatt tgaagaaaaa atggttattt gcagattatt atgatacaac aattatttta
     4861 cttgctttaa tctctgttat tctggtatta cttggatttg ctgagatgac tgatttggac
     4921 aatccgcctt atagtattat tgatttagta atctggggtg tttttgtaat tgattatagt
     4981 tggcgctttt ttattactaa aagaaaatgg cgctttattc ttgaaaatat ttttgattta
     5041 ttgaccattc tccctttaaa tgctattttt acagtgtttc gattaggacg tatcttccgc
     5101 ttagcaaggc tgacaaaatt actgaaactg actcgtttac ttagaataat tggacttaca
     5161 ggtaaactag aaagaaaaat tagtagattc ttacgaacaa atggattgat ttatatcttg
     5221 tatgttaata tctttattgt tctagtaggg agttcgattt tatccgttgt tgaagaaaaa
     5281 tccttctcag atagtctttg gtgggctctt gtgacagtaa ctactgttgg ttatggtgat
     5341 attgttccag tttccctttt tgggaaatgg ctagcagttt tactgatgct tgtcggtatt
     5401 ggaacaatag ggatgctaac aagtgctttg acgaactttt ttgtaaagga taatccagat
     5461 gaacagataa aacttgataa actcaaagac gaattatcga gctaaagaat attaatagag
     5521 aaacaatctg aaaaaatcga agaactacat tgaatgataa aagacttagt tgaaaaaata
     5581 tcataaaggt gagacaaaat aaaacttgat tcgaggagaa atcaagtaag ttttgacaca
     5641 atagaacgca tattatcagg tataaaaact tgatactatg cgttttatag agaaataaaa
     5701 taaaatctga acaaatcaat taggaattta aagcatttta taacaacgtt ttagagtaat
     5761 ggggtgctat ttcaacttca acctactata atacagaaaa aaatacagaa aaaaacaact
     5821 ccctgatgat tcaaggagtt gtctatagtt aaattagttt ttagaagctt cttggaattc
     5881 tggatttttc catgcttcgt caataatagc ttgcaattct ttagcagatg cttgcatttt
     5941 ttgagtttct gcatcgttca atggaatgtt tactggacga acgataccat gtgcaccaac
     6001 aacagctggt tgaccgataa agacattctc aactccgtat tgaccttctt ggaatactga
     6061 aagtggaagt actgcgtttt catcgtcaag gattgcttta gtgatacgag caagggctac
     6121 tgcgataccg tagtatgttg cacctttttt gttgatgatt gtgtaggctg catcacgaac
     6181 accttcgaac aattcaatca attcagcttc ttgaacattt tgagtgtctt taaggaattc
     6241 ttcaaggttt acaccagcga tgttagcgtg tgaccaaaca gcgaactcag agtcaccgtg
     6301 ttcacccatg atgtaggcgt gcactgaacg agcatccaca tccaattttt cagcaagtgc
     6361 ttgacggaaa cgagctgagt caagtgaagt acctgaaccg ataacgcgtt ctttagggaa
     6421 accagagaat ttccaagttg agtaagtcaa aacgtcaact gggttagcag caacaaggaa
     6481 gatacctttg aaaccagatt caacaacttg agttacgatt gatttgttga tagcaaggtt
     6541 tttacctaca aggtcaagac gagtttcacc tggtttttga ggtgcacctg cagtgatcac
     6601 aacaaggtca gcgtctgcac agtcagagta ttgagctgca tagatttttt taggtgaagt
     6661 gaaggcaagg gcgtgactaa ggtcaagcgc atcaccaaca gctttttcat gcaattgtgg
     6721 aatttcgata attccaagct cttgtgcaat tccttggtta acaagtgcaa aagcgtaaga
     6781 tgaacctaca gcaccatcac cgacaaggat aacttttttg tgttgtttag ttgaagtcat
     6841 tgttttaaac atctccttaa ttttattagg ggattttccc tagacaactt cattctatca
     6901 cttttaaaaa actttgtcac gaatatgcct tatagttctc gatgtaaacg ttttagtggt
     6961 ttagaggctg aaatagatgg gaatttatgg tataatgttg ttacttacta attgtgaaat
     7021 gaggcattta ttaatgcagg ataaaaattt agtgaatgtc aatctgacaa aggagatgaa
     7081 ggcaagtttt atcgactacg ccatgagtgt tatcgtagcg cgagctcttc ctgatgttcg
     7141 agatggctta aaacctgttc accgtcgcat tctctacgga atgaatgaat tgggtgtgac
     7201 cccagacaaa ccccataaaa aatctgctcg tattacaggg gatgtcatgg gtaaatatca
     7261 cccacacggg gattcctcta tttatgaagc catggtccgt atggctcaat ggtggagcta
     7321 ccgttacatg cttgtagatg gtcatgggaa ttttggttcc atggatggag atagtgctgc
     7381 cgctcaacgt tataccgagg cacgtatgag caagattgct ctggaaatgc ttcgtgatat
     7441 caacaaaaat acagttgatt tcgttgataa ctatgatgcc aatgaacggg aacccttggt
     7501 cttgccagcg cgttttccaa accttttggt taatggagca actggtatcg cggttgggat
     7561 ggcaaccaat attccacctc ataatctggg tgaaaccatt gatgcagtga agttggtcat
     7621 ggataatcct gaagtgacta ccaaggactt gatggaagtc ttgcctggac cagattttcc
     7681 aactggtgct cttgtcatgg ggaaatcagg tatccataag gcttatgaaa caggtaaagg
     7741 ttcgattgtc ctacgttctc gtacagagat tgaaacgact aagactggtc gtgagcgtat
     7801 cgttgtaaca gaatttcctt acatggtcaa taaaaccaag gtgcatgagc atattgttcg
     7861 cttggttcag gaaaaacgca ttgagggtat cacagcagta cgtgatgagt caaaccgtga
     7921 aggtgttcga tttgttattg aagtcaagcg cgacgcctca gccaatgtta ttctcaataa
     7981 cctcttcaaa atgacccaaa tgcaaaccaa ttttggattc aatatgctcg ctatccaaaa
     8041 tggtataccg aaaattttgt ctcttcgtca gattttggat gcttatattg agcaccaaaa
     8101 agaagtggtt gttcgtcgta cacgttttga taaggaaaaa gcggaagcgc gcgctcatat
     8161 cttagaaggt ctcttgattg cgctagacca tatcgacgaa gtgattcgta tcatccgtgc
     8221 tagtgaaacg gatgcggaag ctcaagctga gttgatgagc aagtttaagc tttctgaacg
     8281 tcaaagtcaa gctatccttg atatgcgtct tcgtcgtttg acaggtttgg aacgcgataa
     8341 gattcaatct gagtatgatg acctcttggc tctgattgcg gatttagcag atattcttgc
     8401 taagcctgaa cgtgtttctc aaattatcaa agacgaattg gatgaagtta aacgtaaatt
     8461 ttctgataaa cgccgtacag agttgatggt tggacagatc ttgagtctcg aggatgagga
     8521 cttgattgaa gaatcggatg tcttgattac cctttctaac agaggctaca ttaagcgttt
     8581 ggatcaggac gagttcactg ctcaaaaacg tgggggtcgt ggtgtccaag gaacgggagt
     8641 gaaagatgat gactttgttc gtgagttagt gtcaactagc acccatgatc atctgctctt
     8701 cttcacaaac aagggacgtg tctatcgtct taaaggttat gaaattcctg agtatggtcg
     8761 gactgccaaa gggctaccag tagtcaatct cttgaaattg gatgaagatg aaagtattca
     8821 gacggttatc aatgttgagt ctgatcgcag tgatgatgct tatctcttct ttacaacccg
     8881 tcacggtatt gtgaagagaa ctagtgttaa ggagtttgcc aatattcgtc aaaatggtct
     8941 caaagcgctg aatttaaagg atgaagatga gttaatcaat gtcttgttga cagaaggaga
     9001 tatggatatt atcattggta ccaagtttgg ttatgcagtt cgctttaatc aatcagccgt
     9061 tcgtggtatg agccgtatcg ccactggtgt gaaaggtgtt aaccttcgtg aaggagacac
     9121 agttgttggt gccagcttga ttactgatca agatgaggtt cttattatca cagaaaaagg
     9181 atatggtaag cgtacagtcg ctactgaata cccaacaaaa ggtcgtggtg gtaagggaat
     9241 gcagacagct aaaattaccg aaaaaaatgg cttgctggcc ggtcttatga ctgttcaagg
     9301 ggatgaggat ttgatgatta tcactgatac aggtgtcatg attcgaacca atcttgccaa
     9361 tatttcacaa acaggacgtg caactatggg agttaaagta atgcgcctgg atcaagatgc
     9421 tcagatagtg actttcacaa cggttgcggt ggcagaaaaa gaagaagttg ggacagaaaa
     9481 cgaaacagaa ggtgaagcat aatgtctcaa aaaaataata aaaagaaaaa caagcgaaaa
     9541 aatctgctga caaatatcct agcaggattt ctgatattac tgtcactggc tttgattttt
     9601 aatactcaaa ttcgaaatat tttcatagtc tggaatacca ataagtatca agttagccag
     9661 gtatcaaaag aaaaattaga agaaaatcag gatacagaag gcaattttga ctttgattct
     9721 gtcaaagcta tctcttcgga agctgttcta acttctcaat gggatgctca aaaattacca
     9781 gttattgggg gaattgcaat tcctgaattg gaaatgaatt tgccgatttt taaaggactt
     9841 gataatgtta atctcttcta cggagctggt acaatgaaac gcgagcaagt aatgggagaa
     9901 ggaaattata gtctagctag tcaccatatc tttggtgttg ataatgctaa taaaatgtta
     9961 ttttctcctt tagataatgc taaaaatggc atgaagattt atctaaccga taaaaataaa
    10021 gtttatactt atgaaatacg tgaagtcaaa cgtgtgacac cggatcgtgt tgatgaagtt
    10081 gatgatagag atggggtcaa tgaaatcaca ttagtaacct gtgaagacct tgctgctaca
    10141 gaacgtatta ttgtcaaagg tgatttgaaa gaaacaaaag attattcaca aacatctgat
    10201 gaa
//
LOCUS       DAVKFH010000071        10130 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKFH010000071 DAVKFH010000000
VERSION     DAVKFH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.071"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..211
                     /locus_tag="VW006_001812"
                     /pseudo
     CDS             1..211
                     /locus_tag="VW006_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            309..521
                     /locus_tag="VW006_001813"
                     /pseudo
     CDS             309..521
                     /locus_tag="VW006_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            421..867
                     /locus_tag="VW006_001814"
     CDS             421..867
                     /locus_tag="VW006_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9380622.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1005..2309)
                     /gene="eno"
                     /locus_tag="VW006_001815"
     CDS             complement(1005..2309)
                     /gene="eno"
                     /locus_tag="VW006_001815"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9380623.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2473..2919
                     /locus_tag="VW006_001816"
     CDS             2473..2919
                     /locus_tag="VW006_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9380624.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2916..3719)
                     /locus_tag="VW006_001817"
     CDS             complement(2916..3719)
                     /locus_tag="VW006_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9380625.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3728..4057)
                     /locus_tag="VW006_001818"
     CDS             complement(3728..4057)
                     /locus_tag="VW006_001818"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9380626.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4002..4373)
                     /locus_tag="VW006_001819"
     CDS             complement(4002..4373)
                     /locus_tag="VW006_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9380627.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4432..5865)
                     /gene="glgA"
                     /locus_tag="VW006_001820"
     CDS             complement(4432..5865)
                     /gene="glgA"
                     /locus_tag="VW006_001820"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9380628.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5862..7001)
                     /gene="glgD"
                     /locus_tag="VW006_001821"
     CDS             complement(5862..7001)
                     /gene="glgD"
                     /locus_tag="VW006_001821"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9380629.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6991..8133)
                     /locus_tag="VW006_001822"
     CDS             complement(6991..8133)
                     /locus_tag="VW006_001822"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9380630.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8123..10051)
                     /gene="glgB"
                     /locus_tag="VW006_001823"
     CDS             complement(8123..10051)
                     /gene="glgB"
                     /locus_tag="VW006_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9380631.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3082 a         2236 c         1794 g         3018 t
ORIGIN      
        1 gaaccacacc tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa
       61 tagtttaaag cacctagcac aggtctaaca aatgaatggt gagttaatcg ctccaatgac
      121 ttacgaagag acgatgacga gcgacttttt tgaagcttgg tttcagaaat ttctcttacc
      181 aacattaacc gcaccattgg ttattatatt aaaacgaagt aagaatagga caaatcgatc
      241 aggacagtaa aatctaattc taacaatgtt ttagaagtag aggtgtacta ttctagtttc
      301 aatctactat attaatggac aatgcaagat tccatagaat ggggaagcta gaactcttgt
      361 gtgaagagtt tgggcataaa cttttacctc ttcctcccta ctcgcctgag tacaatctta
      421 ttgagaaaac atgggctcat atcaaaaagc acctcaaaag ggtattacca agttgcaata
      481 ccttttatga ggctcttttg tcttgttctt gtttcaattg actatataaa ttgtctaagc
      541 gaaacaaccg ataagaattg gcacaaaagc gaccgtattt ttgttaccaa tacaggaaaa
      601 ccagttcata gttctatctt gagcaagtct ctccagcgag caaacgaacg ccttaaaaaa
      661 ccaattccca aacatctgtc ccctcacatc ttcagacaca ccactattag catcttatca
      721 gaaaataaaa ttcctttaaa aacaatcacg gacagggttg gtcattccga ctctgaagtc
      781 actacttcca tctacaccca cgtcacaaag aacatgaaag atgaagcaat caatgtactg
      841 gataaagtta tgaaaaagat tttttaaaaa gttttgtccc ttttttgtcc tctaaataca
      901 aaaatagccc ttcggataaa atccgagggg ctagaaacgt tgttaaatca acggccgaac
      961 ttttgaattt catggttcgg gataaaatag ttcactgaac tattttattt tttaaggttg
     1021 tagaatgatt tcaatccacg atattcagct acttcaccaa gttggtcttc gatacgaagc
     1081 aattggttgt atttagcgat gcggtctgta cgtgaaagtg aaccagtctt gatttgtcct
     1141 gcgttagttg caactgcaat atcagcgatt gttgaatctt cagtttcacc tgaacggtgt
     1201 gatacaacag cagtgtaacc agcttcttta gccatttcga tagcttcaaa agtttcagta
     1261 agagtaccga tttggttaac tttgataagg attgagttag cagcaccttc ttggatacca
     1321 cgtgcaaggt agtcagtgtt tgttacgaag aagtcgtcac caacaagttg tactttctta
     1381 ccaagacgtt cagtaagagc tttccaacca tcccagtcgt tttcatccat accatcttca
     1441 atagtgatga ttgggtattt gttaaccaat tcttcaaggt agtcgatttg ttctgcagat
     1501 gtacgaacag cagcaccttc accttcaaat ttagtgtagt cgtaaacttt acgttcttta
     1561 tcgtagaatt ctgatgaagc acagtcaaat ccgataaata cgtctttacc tggtacatat
     1621 ccagcagctt caatcgcagc aaggatagtt tcaacaccat cttcagttcc ttcgaaacga
     1681 ggagcgaatc caccttcgtc acctacggca gtttccaaac cacgtgattt aaggattttc
     1741 ttaagagcgt ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt tggcgcacca
     1801 actggcaaga tcatgaactc ttggaaagcg attggagcgt cagagtgaga accaccgttg
     1861 atgatgttca tcattggagt tggaagaact ttagtgttga atccaccaag gtagctgtaa
     1921 agtgggattt caaggtagtc agcagcagca cgagctacag cgatagacac accgaggatt
     1981 gcattcgcac ccaatttacc tttgttagga gtaccgtcaa gtgcaatcat agcacggtca
     2041 atagcttgtt gatcacgtac atcgtagcca atgatagctt cagcaatgat gttgtttacg
     2101 ttgtcaacag ctttttgtgt accaagacca ccgtaacgag atttgtcacc gtcgcgaagt
     2161 tcaactgctt cgtgttcacc agtagaagct cctgatggaa ccataccacg tccgaaagca
     2221 cctgattcag tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc taggacttcg
     2281 cgagcgtaaa catcagtaat aattgacatt ttttactctc cttatgagtt aaatttttta
     2341 cacctctata ataccttaaa acccctcctt tttcaagaaa aaacgttatc tttgtgcaac
     2401 ttttccttaa ctttataaag taatcgcttt cttttgtctg ttttattcta acttttatga
     2461 tatactgttt tcatgacaga tttatcaaaa caattacttg aaaaagctca tggtgggtta
     2521 aaaataaatc cggatgagca aagacgctat cttggtactt ttgaggaaag agttcttgga
     2581 tatgtagata ttgacacagc aaatagccct cagttagaaa aaggcttttt atttatttta
     2641 gaaaaccttc aggaaaaagc aaagccacta tttgtgaaga tttcaccaac tatcgaattt
     2701 gataagcaag ttttctactt aaaagaagca aaagaaactg atagtcaagc caccatagta
     2761 tctgaagagc atattacttc tccttttggc ctggttattc atagcaatgc accagttcaa
     2821 gtagaagaaa aagaccttcg acttgctttt ccaaaacttt gggaagttaa aaaggaagaa
     2881 ccagccaaaa catccttatg gaagaaatgg tttagctaaa tcttgcacat atttaataag
     2941 tgcccaatat tggcagccgt gcgctccaga tagagactgg catttttcaa actatcttct
     3001 aaaggttcac ttttctccaa aatagaaaag acagcttgga tattttcaaa tggtagggga
     3061 ggtaaatctt cagcaagact accgcaaata gcaataacag gaactccaac aggggttctt
     3121 tttgcaacac ctataggcgc tttcccagca aagctttgac tatcaagtct tccttctcca
     3181 acaacaacca agtcagcatc tgaaactttc ttatcaaagt tgattaagtc caagcaggta
     3241 tcaattccag acacgatact tgcctgagca aaggcacaca aaccagcagc aatccccagt
     3301 cctccatcat tactggccgt gccaccaaca ccgatataaa tatctttaat ccctttagaa
     3361 atgagatgga gaatcaactc tccaatacca caagtttgga tttgaagtgg atttcgtttc
     3421 tctagcggaa tttttccaag accaaccaag tcagctactt caaatagtgc cagttcccct
     3481 ttttgaaaat agcgcatggc ttctttttgt ccaaaagggt ctgtcacttg gatccatttt
     3541 tcttttaggt caagagaatg tcggatagca tctacagtac cttctccccc atcaccaaca
     3601 gggcagagga gacattctac atctgctatc gattgttgga agcctctttt tattgcttca
     3661 gctacctgtt gagctgtcaa gctttcctta aacgaatccg gtgcaattac aatcttcata
     3721 ttttccctca ttctaaacag tcaatcaaag gaagaacttc taaaaaatcc ctcttgtcaa
     3781 catgatgtgg tatttctttt ttgagcattt ctttggaaca aaaggcgatt cctaactccg
     3841 ccgacttcaa cattaataga ttattaaccc catcaccgat tgccaccgtt ctttctttag
     3901 aaagttttag tttctttctc catttttcca gagtttcttt tttgacctgg ggacttataa
     3961 tttgtccaac taattttcct gttaaaagac cttctttgac ttcaagctgg ttggcagtga
     4021 aataggcaat accaagggat tttgctaatc tctccaacta ttggtgtaaa tccaccagac
     4081 accagaccaa ctaggatgcc attcttttgg agaatagaga tgaactctgg gacatttagc
     4141 gatagatgaa ttgagttgaa gacgttatca aagaccaaaa taggaagacc ttccaacaag
     4201 gacactcttt ttcttaaact gctttcaaag accaactctc ctcgcattgc tcgacttgta
     4261 atctgcgaaa tttccgcctc atgacctgcc tctctcccta aaagatcaat cacttcttct
     4321 aggattaagg ttccatctac atccaaaaca cacaagcctt ttacttgaga catcagttct
     4381 cctctctaaa cagcctaaaa atcgtatgaa gtcatcatac gattttatct attaattaac
     4441 taaactatgg tacaagtcaa ggtatgactt gcaggctgta tcccatgaga agtcactctc
     4501 catagcttgt ttttgtaggt ttctccaaat gtctggatgg tttctataca agtccaatgc
     4561 tgtttggaaa gtccaattta accaataagg agatagattg tcaaagctaa agccagtacc
     4621 gcttccttcg attggattga aagcgcgaac tgtatctcgc aagcctccaa cttcatggac
     4681 caatggcaag gttccataac gcatagccat catttgagac aagccacacg gttcaaaacg
     4741 acttggcatg aggaagaggt cacaagcagc gtagatttcc tgagcaagtt tgacatcaaa
     4801 agtgatattt gctgatagct tgtctgggta aatctgagca aaccatgaga aagctccttc
     4861 aaaggctgga tcgccagttc ccaaaagaac aatctgaaca tcttcttgca agatatggtg
     4921 aagactttcg accaccacat caaaaccttt ttgacgtgtc aaacgagaaa caattcccac
     4981 cagtggaacg tctgctctaa caggcaagcc aactctttct tgcaattttg ccttattttt
     5041 ggctttccca gacaaatctt cctgattgaa atgatagtct aaaagagcat ccgtctgagg
     5101 attataaaga tcagcatcaa tcccattcac gataccagat actttaccag actccatttt
     5161 aagaatctga tccaaattac atccaaactg actagtcata atttcatgag catagctagg
     5221 tgaaacggtt gaaacacggt tcgcatagag aatacctgcc ttcatccagt tcagacagtt
     5281 gttccatcga agggtgccat cagcgtaacg ttcaaggcca actccaaaca aatcacccaa
     5341 cattccttct gaaaattgtc cttggaattc taaattatga atggttaaaa ctgtttcaat
     5401 gtcctcatag gcttgaatcc aacagtattt ttccttcaac aagaaaggaa tcatagctgt
     5461 atggtagtca tgaacatgga gaagatcagg aataaagtca atcctttcca tagcctcaat
     5521 ggcagccagt tggaaaaagg caaagcgttc tccgtcatca aaatcaccgt aaacatgacc
     5581 acggaagaaa taatattgat tgtcaataaa gtagaaggtt acaccattta atactgtttt
     5641 cttaattcca caatactgtc tacgccaacc aacgctcacc tcaaaatgaa gcacatcttc
     5701 aatctgattt ccaaatttag cctctaccat atcatagtag ggtaaaatca ctgcaacttc
     5761 gtgcccagct tttaccagtg attttggaag agcgccaatg acgtctccca aaccacctgt
     5821 ttttgaaaag ggtgcaccct ctgctgctac aaataaaatt ttcatgaatg aatatcctct
     5881 gttactttag cacctttctt aaccacaact ggatgttctg cagttcctcg aatcacaaca
     5941 ccaagctcaa cttcaacccc tttgtccaag atagcatatt cgacctgagc cccttctcca
     6001 ataacaacac gagggaagag caggctatct ttaaccaagc tatccttatg gacatgaata
     6061 ttacgtgata gaacagaatt agctacttga ccttcaataa tactaccaga ggcaaactga
     6121 gaagtgctta ccttagatgt attagcatag taagttggct cttcgttttt gacctttgta
     6181 taaatctttt ggtttggtga gaaaagagaa tagaattttt gtgattcaag catatcgata
     6241 ttcgcttgat aataagattt aacagagtga atattggcta gatagcccgt gtactcgtag
     6301 gcaaaagctc cctcttttac agccaaatcc cgtaaaacat agcgcaattt ctctggatgt
     6361 tcttttttag cttcttcttc caagtgttca atcaaccaag gtgtatcaac gacaaagata
     6421 tctgtagaca tattgaacgt ttcagctgtt gacttgctat caaagagttt atgagaaaga
     6481 acatggtctg tttcatctac atccaagatt gcatttactt ctgaaatatc tttcttagct
     6541 agttttttat aaactacagt gataggctct tttgttgtac tatgtaggtg gaaaacttgg
     6601 ttcaaatcaa tgttaataag aacatcgcag ttgagggcaa ccgtttggtt tgagccagaa
     6661 cgtttcaaat aagtaagaag ctgttggtag tattcttttc caactgtact actttctaca
     6721 cgggtattgt aaattcctag atagtaatgg ctaagaaggg ttgataagcc ccactcgcgc
     6781 cctgaacgaa tatggtcaaa gactgagctg atattatcct gctggaaaat accaaagaca
     6841 ctacgaacac ctgcattagc aaggcttgaa agtgggaagt caatcaaacg atatttccca
     6901 ccaaatggca aacttgctac tggacggtgg tccgtcaatg tcgacatatc gtgaaaacca
     6961 actgtatttc ctaaaatggc agaatattta tcaatcttca tctgttgcta cccccactac
     7021 ttcattatat cctacaactt gtacttcatc tgttccatca atttcgacac cgtcagaaat
     7081 aatcgcacct tcaccaataa tggcacgttt aatcttagct ccttgaccaa tgatagctcc
     7141 actcatgata actgaatcaa ggacttccgc tccttcgcga acttgcgcgc ctgttgaaag
     7201 gatagaatgt ttaacagttc catcaacgaa acatccgtct acaactaatg agtcttccac
     7261 atgagcattt gccccgagga agtttggtgg tgaaatcaag tttcttgagt aaatcttcca
     7321 ttgacggtta cgactatcca aggcattttc tggagaaata tactccatgt tcgcttccca
     7381 aagtgactca atagtaccaa catctttcca ataaccacta aattcataag cataaacact
     7441 ttcacctgac tcaaggtaat ttggaatgac atttttacca aagtctgaca tgccaacctt
     7501 gctcttttca gcagcgacta acatattacg aaggcgttgc caatcaaaaa tgtagattcc
     7561 catagaagct tttgtagatt taggttgagc tggtttttct tcaaattcaa caatacgatt
     7621 gttagcatct gtgttcatga taccaaaacg gcttgcttct ttaagaggga cgtctaaaac
     7681 tgctactgtc aagctggcat tattatcctt atgagactgg agcatatcat catagtccat
     7741 tttgtagatg tgatccccag acaaaatcaa gacatactca ggattgacac tgtcgatata
     7801 gtcgatattt tggtaaatag cgtgactagt cccctcaaac caacgatttc cttcacttgc
     7861 agaataaggt tgaagaatag agacacctga attaataccg tctagtcccc agcttgaacc
     7921 attcccaata tggttgttga gagcaagtgg ttgatactgt gtaacgaccc caacattatg
     7981 aatccctgag ttggcacagt ttgatagggc aaagtcaatg atacggtagc gcccaccaaa
     8041 ttgcacagct ggttttgcga tgctttgagt gagtttaccg agacgagttc cttgcccacc
     8101 agcaagaatc aaagctaaca tttcattttt cattttctac tcctttttgg tttttatttg
     8161 tgacggtttt agtagatttc aagcgacgtt tgattttcca tacacttgct cccatagccg
     8221 gtagggtaaa ggttaaggtc tgctcataat ctttccatag tccttcttgc gtttgaacag
     8281 tttgattatg ttctttccaa acgcctcccc actcttccaa ctcagtattc catacttctt
     8341 cgtaaattcc tgcaacgggt agtccgattg taaaatcttt ccgctcaaca ggtaccatgt
     8401 taaagataca gactaacatt tctccctttt tacccttacg aataaaggaa agaacactct
     8461 ggtctcgatt atccgcatca atgatttcaa taccatcata gctggtatcg atttcccaca
     8521 gacagcgatg atctttgtaa aactggttta gctgagaagt gaaatacttc atcttagcat
     8581 tcattgggtc ttctaggtta gaccattcca actgttcttc agatttccat tctaggaatt
     8641 gaccgtattc gctacccatg aagagcaatt tcttaccagg atgacaaatt tggtacgtat
     8701 agagattgcg caagcctgcg aattgattgt aacgatctcc ccacatctta tgcatcatac
     8761 tcttcttgcc atgaaccact tcatcgtgcg agaatggcaa gagataattc tccttgaaaa
     8821 catacataaa gctgaaagtc accaggttaa agtcatattt acgatagatc ggatcttctt
     8881 cgtagaaacg gaggatatca ttcatccagc ccatgttcca tttgtagtca aatcctagac
     8941 caccaatctc tttcattccc gtaatcttgg tcgcagacga actttcttct gcaatcatca
     9001 tcacatctgg atattctaac ttaataacct cattcaagcg ctgaaggaaa taataacctt
     9061 catagttgag atttccgcca tctttattag gtgtccatgg agcatcatca tagtccaaat
     9121 agagcatgtt gctaacagca tccacacgaa taccatccaa atgatagaca tcaatccaat
     9181 gcttaatgca agaaattaag aaggactgga cttcattttt tccaaggtca aaattaaggg
     9241 caccccaacc atggttatga gccttattat ggtcttggta ttcaaaagtc ggtgtcccat
     9301 cataataggc taaggcatca tcgttgatgg taaagtgacc tggtacccag tccacaataa
     9361 ccccaatatt atgggtatga cactcctcga caaaatcttg aaactcctct ggtcggccat
     9421 aagcatgctc taaagcgaag taacccataa gctgataccc ccaactcaag cccaaaggat
     9481 gggacatcaa gggcataaac tcaatatgag tatagttcat ttcaacgaga taaggaatga
     9541 gttcatcctt gagctgggca aaactataag gactgccatc agaatttctt ttccatgatc
     9601 cagcgtgaac ttcataaata ttgacaggac gctcttcaaa gccccaacgt tttcttcgtg
     9661 ccagccaaag tccatccttc catttcttct cacgaagctc tgttaagatt gcccctgttc
     9721 ctggacgagc ctcatacctg acagcaaaag ggtcaatctt catcagttga tgaccatttt
     9781 gacgtgtgac atgatatttg taaatatgcc cttcttgagc catattggta aagacttccc
     9841 agaccccaaa atcatttctt accattggaa tctgattttc aatccagttg gtaaaatcac
     9901 caaccaagtg aacagcctga gcattaggtg cccaaacacg gaaggtatag ccatgctctc
     9961 catttagttc ttccctatgt gctcctagat aatgttggag ataaaaattt tcacccgtca
    10021 taaaggtttt taatgcttct ctattatcca tatactcccc ttctcctgta agcgttttct
    10081 atgtttttat tatactacct ttttagagaa gattcaagta aattactata
//
LOCUS       DAVKFH010000072         9776 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKFH010000072 DAVKFH010000000
VERSION     DAVKFH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.072"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..120
                     /locus_tag="VW006_001824"
                     /pseudo
     CDS             1..120
                     /locus_tag="VW006_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756110.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            343..536
                     /locus_tag="VW006_001825"
                     /pseudo
     CDS             343..536
                     /locus_tag="VW006_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            596..832
                     /locus_tag="VW006_001826"
     CDS             596..832
                     /locus_tag="VW006_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9380632.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            829..1242
                     /locus_tag="VW006_001827"
     CDS             829..1242
                     /locus_tag="VW006_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9380633.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1360..2325
                     /locus_tag="VW006_001828"
     CDS             1360..2325
                     /locus_tag="VW006_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9380634.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            2291..2839
                     /locus_tag="VW006_001829"
     CDS             2291..2839
                     /locus_tag="VW006_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9380635.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSS
                     GKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWIS
                     DNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQ
                     NEFADFVALVDKSQIYYPCFKE"
     gene            2799..3038
                     /locus_tag="VW006_001830"
                     /pseudo
     CDS             2799..3038
                     /locus_tag="VW006_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            3068..6418
                     /locus_tag="VW006_001831"
     CDS             3068..6418
                     /locus_tag="VW006_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9380636.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(6924..7394)
                     /locus_tag="VW006_001832"
     CDS             complement(6924..7394)
                     /locus_tag="VW006_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9380637.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(7411..9684)
                     /locus_tag="VW006_001833"
     CDS             complement(7411..9684)
                     /locus_tag="VW006_001833"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9380638.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVILQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         3165 a         1591 c         2034 g         2986 t
ORIGIN      
        1 gagcaacaaa gaattgcaag tgagctggat ttgttaagca agctgatttt aaggagacaa
       61 gaacaacttg aagagcttaa tctacttgta aaatcccgat ttaacgagat gtttggggaa
      121 aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga taggggcaaa
      181 aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt aaatacaaag
      241 aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa aacaaaggat
      301 aaattacttc gaaaaggcaa acttgagcgt tatgatatag tcttgacaac aagaggtact
      361 gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg tataaattca
      421 ggtatggtaa tattacgtcc caagacacca aatctaaatc acaattggca atccaaaaat
      481 ctctggaaga acttgaaact ttgaagaaat ctctgatgca ggagtatttt ggctgatatt
      541 ctgccattgt aattacggta atgatttgtt ataatacttc aaaggaggaa atcagatggt
      601 agtaaaaaca agaaaacaag gaaattcaat caccattacg attccaagtg aatttaatat
      661 tccaagtggt gttaaatacg aagcgaaatt gttaccaagt ggtgagatta tctttactcc
      721 tgaagaattg gggcagcagg tttcttatgt atctgatgat gcctttgact taaatttaga
      781 taaaatattt gacgaatacg acgatgtttt caaagctttg gtggaaaaat gacaatctat
      841 ctgacagaaa agcaaattga aaaaataaat gctttagcaa ttcaacggta ttctccaaat
      901 gagaaaattc aaacagttag ttcttctgcc ttaaatatga ttgtgaactt accagaacaa
      961 tttgtctttg ggaagtctct ttatccaaca atttttgata aagcaacgat actatttgtc
     1021 caattgataa agaagcatgt ttttgctaat gctaataaaa gaactgcttt cttcgttttg
     1081 gtcaaatttt tacaattaaa cggctatcgt ttttctgtaa cggtagaaga agcagtaaaa
     1141 atgtgtgtaa ccatcgcagt agaagcttta actgatgaaa aaatgacaag ctactccaaa
     1201 tgggtttctg aacattctgt tagagaaaag gtcaaaaagt aacctagtat gctggatttg
     1261 aatgagcaca agaaaataaa tgaatagaca atattagaat tctgtaatgc agaaactgat
     1321 attgtctctt tttattgatg aataagaaag tgagaaatta tggaatcaaa agttacaatt
     1381 atcatgcaag aaatgttacc tcttttaaat aatgaacaat tactagcgtt gagagagagt
     1441 ttagaacatc atctagtaga cggaaaaaag cagcagaagt attcgaataa taacctgttg
     1501 caactattta ttaccgccaa gcaggtagag ggctgtagct caaaaacaat tcgttattac
     1561 cagaggacga ttgaaaactt gtttaatgct attaaagagt ctgtgacaca actcacaaca
     1621 gatgatttaa ggagttattt agcaaattac cagtctgaaa aggattgtag taaggcaaat
     1681 ttagacaata ttaggcgtat attgtcttct ttttttgctt ggcttgagca agaggaatat
     1741 atcattaaaa atcccattcg acggataaag aaaattaaga ctgagcaaaa tgtgaaggaa
     1801 acttatactg atgaacattt ggaaattatg cgtgataact gtgaaaattt gagagatttg
     1861 gcaataatag acctactagc atcgacaggt atgcgtgtag gggagcttgt acagttgaat
     1921 cgttcagata ttgattttga aaacagagag tgtgttgtct ttggtaaagg aaagaaggag
     1981 agaccagtat attttgacgc tcggacgaaa attcatttaa gaaattatct taacgacaga
     2041 aaagatagtc accctgctct ttttgtaacg ctagttggaa aagcccagag acttggaatt
     2101 gctggtgtag agattcgctt aagaaagtta ggagacaaac tcggcataca aaaggttcac
     2161 ccacataagt tcagaagaac tttagcgact aaggcaattg ataaaggtat gcctatcgaa
     2221 caagtccaaa aactgctagg tcatagcaag attgacacaa ccctggccta tgccatggtc
     2281 aatcaaaata atgtcaagca ttcacaccaa aaattcatct cttaaaagca aatcccgatt
     2341 taacgagatg tttggggatc ctttaaataa taacaagaaa tttgcagtaa aaacgggaca
     2401 acagtgtttt aaattttcaa gtggaaaatt tcttgataaa catgatagag tttttgaagg
     2461 ttatcctgcc tatggtggaa atggcattgc ttggaaaagt cgaaaatatt taattgataa
     2521 tcctactatc atcattggtc gtgtaggtgc atattgtggt aatgtaagaa ctacccacgg
     2581 taaggtatgg ataagtgaca atgctatata tataaaagaa tttaagaatt ccgatttcaa
     2641 tcttgtattt ttattagaat taatgaaagt aattgatttt agtaaatttg ctgatttctc
     2701 aggtcaacca aagataactc aaaaaccatt agaaaatcag aaatatatcc tcccccctct
     2761 agccctccaa aatgagttcg cagactttgt agccctggtc gacaaatcac aaatttatta
     2821 tccatgtttt aaggaataat aattatagtc gagtgatatc aggaagtgct cagcctcagt
     2881 taccaattac aaaattaaaa aaaatacttc tccccctccc cccactagcc ctccaaaatg
     2941 agttcgcaga ctttgtagtc caggtcgaca aatcagaatt tgcttgtgag atagctataa
     3001 aagtgtggag aaatagcttg aaatttagta taatatagct aaactatttg tttaaagtga
     3061 gaaaaaaatg ggaaatttta gctttctttt aaaaaatgac gaatatgaat ctttttcaaa
     3121 accttgcatt gaagctgaga atatgattgc tacatcaact gtggctactg cctttatggc
     3181 gcgtcgtgct ttagagcagg ctgtccattg gatatatagt cacgattcat atttagaagc
     3241 tccctatcgt gctactctat cttctttagt atgggatgat gattttaggg atatcgtaga
     3301 ttctgaactc cacaagcaga tagttctgtt gattcggtgg ggaaaccatg ctgctcatgg
     3361 tggtgaaatt aaggaacgag aagcgatttt agctttgcat catttgtatc agtttgttaa
     3421 ttttatcgat tattgttaca gcaatgagtt tgtggagcgt tattttgatg agaagtgctt
     3481 accactttca gcaaacatca aataccgaga aactccacaa tctatgataa agttacaaga
     3541 cagtttacca gaactgcctg attttcatga acagatggct gctcagtccg tagaagttca
     3601 agagacttat actgaaaaac gtgagactgc agcgcaacgg caagatgtgc ctttccatat
     3661 tgatcaatta tctgaggcag agacaagaaa gctctttatt gatatcgatc tccgtttagc
     3721 aggatggata tttgaagaaa actgtcgtgt tgagatagcc gttgatggtc tcaagcacgg
     3781 ttcaggaatt ggttactgtg actatgtact ttatggtaaa aatgggaaaa ttttagcgat
     3841 tgtagaggct aaaaaagcct ctgtcaatcc agaagtaggg gaagtacagg tcaaagaata
     3901 tgctgaagct cttgagaaac atatcggcta tcagccaatt tgctttatta caaatggctt
     3961 gaagcactat atacttgatg gtccgaaccg ccgccagatt gcaggctttt actctcaaga
     4021 agaattgcaa ttagtgatgg atagacgtca tcttcaaaaa ccacttgagg atatttctag
     4081 taaaattagg gacgatattt ccgggcgtca ctaccaaaaa catgccattg caagcgtttg
     4141 tgaagctttc tctaatcatc gtagacaggc acttttggtt atggcaactg gggctgggaa
     4201 aactcgtaca gcagtttctc tagttgatat cttatcacgt cataactggg taaaaaacgt
     4261 tctcttctta gccgatagaa cttccttggt taagcaagcc tatgattcgt ttagaaaatt
     4321 actcccagat ctttccgttt gtaacttctt agaagataaa gaaggagctc aatcaagtcg
     4381 catggtcttt tcaacttatc cgaccatgat tggagcgatt agtggtcaag aagaagtaaa
     4441 tcaacgccct ttcactgttg ggcattttga ccttatcata attgacgaat ctcaccgttc
     4501 tatttatcag aaatacaagt ccatttttga ttattttgat gcaagaattg taggcttaac
     4561 agctactccg cgtcaagatt tagataaaaa cacctatgga ttctttaatt tggagaatgg
     4621 ggttccaaca tatgcatatg atttggaaga ggctgttaaa gacggatatt tagtagccta
     4681 tcattctatc gaaaccaaac tgaaactacc tacggatggt ctacattatg atgatttgtc
     4741 cgaagaagaa aaggaacatt ttgatagcaa atttgaagac gatagctgtg aaaaagatat
     4801 tgatgggagt gtatttaatt cctttgtttt caataaaagt acagtagaaa ttgttttaaa
     4861 tgaactcatg acaagaggaa ttcagacagc ctcgggtgat gaaattggta aaactattat
     4921 ttttgctaaa aatcatgatc atgcggaata tatcagaggt atttttaaca accgctatcc
     4981 tgaaaaaggg agcgactatg ctcaggtgat tgattatagt attaagcatt atcagacctt
     5041 gattgatgat tttaaaatta aggagaagta tcctcaaatt gcgatttctg tcgatatgtt
     5101 agatacaggt attgatgtac cagaggttgt taatttagtc ttcttcaaga aagtacgctc
     5161 taaaactaag ttttggcaga tgattggtcg aggaacccgt ctatgtaaag atttatttgg
     5221 acctgagcag gataaggaaa acttcttggt atttgattat ggggacaatt ttgattattt
     5281 tcgtgcagat ccaagagatg gagagggtcg tcacattgtt tcgttgactc agcgtttatt
     5341 taatatcaaa gtggacttga ttcgagaact tcagggactc caataccaag aagatcagtt
     5401 tgcgagagca taccgtcagc agcttgtctc ggaacttcaa ggtcgtatag agagcttaaa
     5461 tgagttggac ttcagggttc gtatggtttt agatacagtt tatagctata ggaaattgga
     5521 aagttggcag aatctaactg ctgttacaag tgaaaccatt caaaaaaatc tctctctgct
     5581 tttatttgat gaagataaag aagatgagat ggcgaggaga tttgatttgt ggttgcttca
     5641 tattcagttg gggcaactga cagctaaatc ttccactgtt catatttccc aagtgatgaa
     5701 gacggctaga gctctttctg ctattggcaa tatcccgcag gtttttgagc aggctgaaat
     5761 tatcaggaaa gtacaggagc ctgaattttg gaaagaagtt aacttgtctg atttggaaaa
     5821 aattcgtctt gctattcgag atttattaca gtttttggat aaaacagacc gtaaacccta
     5881 ctatgttaac tttgaagatc gtatactctc cactgttcac gagaccacag catttttgca
     5941 ggtcaacgat cttcggtctt acaatgaaaa agttgagcat tatttgaaaa ctcatctgga
     6001 tgaggagtcc atttctaagc tataccataa taaaaagttg acatctgatg atatgcttgc
     6061 acttgaaaaa ttgctttggg aaaaattagg tagtaaagca gactaccaaa gtcattatga
     6121 aaataaggca attccgagat tggttcgtga gattattggc ttagatagag agtctgccaa
     6181 tcgtattttt tctaaatttt tgtcggatga gaatcttaat gccaggcaga tttcatttgt
     6241 aaaattgatt gtagactaca ttgtagaaaa tggtttttta gagacgaaag tgttaacgca
     6301 agagccgttt aaatcttatg gttctgttca actactcttc caacaccaac taccagtact
     6361 tcgtaatatt gttcaaatca ttgaacttat caataatcga gctggagaag cggcttaaat
     6421 tctaaagtga ttgccatgct gagactcatt taaaattaaa aagagtagaa atttatgcta
     6481 tatatgagaa gttttattag gaagaatgtc atcgttttcc tagaatacag tatcagttgt
     6541 taagtggttg ataaatttca aagtagatac ttgtaccacg atgtttgttg atcgagttat
     6601 taacaaaaga gctactttga ttttaaagaa atagaaaaca aaaagccgag caagaattca
     6661 attgcaggag aaaatgaaat aatactcaat gaaaatcaaa gagcaaacta ggaaactagc
     6721 tgcaggctgc tcaaaacact gttttgaggt tgtagatgga agctgacgcg gattgaagag
     6781 attttcgaag agtataaatc ttcctaggat aaagcaaaac gcatagtatc aagggttttc
     6841 aacacttgat actatgcgtt ttctgatgtt aaagactttc taccaggttt tttaaaagca
     6901 taattgttag ttgtagtcat ttattattct tcaaagaaaa atggtggggc gaattttttc
     6961 agttcttcaa agcacttttg agcagtatct gcatcttcac agatgataag acagacatca
     7021 ttaccacaaa gggtagcgat agcgtcaggg aagctcaaag tatcaatgat agaaccaaag
     7081 gattgagcca gtccaggaag ggtttttagt aggacttggt gttgaactgg gcgcatccag
     7141 acaagggcgt cttccatgta gagttcgaga cgtttttccc attttgagat ggaaccattg
     7201 ttaagaacat aataagcgct atcttcttcg cggacttttg ataggttcat atttttgatg
     7261 tcgcgtgaga gggttgcctg ggttacttga atgtcgttct cagcaagaag ggcttgcaac
     7321 tcagcctgtg tatgaatctt gttttttgtg ataagagcgc gtataagttg gtggcggtgt
     7381 tctgatttat tcataataat gtaactcctt ttagcaaggt aaggtaagca tggactgagc
     7441 gaggtcgaca gtcaagtggt agtctgtatt gtcacggatg gtgatttcaa agtcagtagt
     7501 atagaggact aaacggagag tgtctccttc ttttagcttg taaatagttg gctgcagttc
     7561 aaattgaacg tccatccatt catctgcagt aatatcctct actaacagta aatcatttct
     7621 attttgtaaa ttaaggtaac cttttgtcac gactcgttgt gcctctggtc taaatggcaa
     7681 ttcacagaga ttttccaaca tgtgatagcg accgttgtca atggttctag cacttaaaat
     7741 agctggataa ggttgtaggt atttcttttg cccaaattct agcagttggg cagataagag
     7801 ccccttgttt gtactggatt tgatacgaag attgagctga gcgcgaccgt ttaggtggag
     7861 atctttagtc acaggaaggt taatagtaat ctgattggct ttcccttgat agagctctgt
     7921 attgaaggtt tggtatgtct taccatagcg ctcaaaatcc ttatctgggt actggttttg
     7981 aatagcttgc tcttcttgac caagtgagaa ggtttcacag ttttcttgcc caccgaagtt
     8041 atcaagtgat aaccaagtct gtggagctgt attgtcctgc aagataacag taggaagttg
     8101 aaagtctgtt tcctgtccta gtaatttctt ggtcaataag gcatttatgg actcacggaa
     8161 gtcaattgat tgccaattgt tcatgtaaac atgggcacca ttatggaaaa agagatgctt
     8221 gtgtatatga gtaggaagag catggaacat ctggtaaaca tgaagtggtt tgacattcca
     8281 atcctgagaa ccatgagtaa agacaacctc tgcctttact ttatgggcat tgagcagata
     8341 attgcggtca tgccaaaact gattgtagtc cccagttttt cggtctagct gagctttcac
     8401 tttttctaag tcagcttggt gagcttcatt gccacggata tagtcgccag ctaagagatt
     8461 acgagaatag gttaactcag caagggagtc aaagtcctca cctggataac cacctgggct
     8521 agtcaccaga ccgttttcac ggtagtagtt gtaccatgat gaaattcctg cctcggcaat
     8581 gataacttct aaaccatcga ctcctgtagt cgcaagacca ttggacatgg tacctagata
     8641 ggaaagtcct gttgtagcaa cttttccgtt tgaccaatca gccttgactt gacgctggcg
     8701 cgtgtgatca gtaaaggcac ggcaacggcc gttaagccaa tcgatgacat ttttataagc
     8761 ctcgatttgc tggtagtctc cattagtcat gaaacctgtc gagtctttgg taccaacacc
     8821 tgagacatag agattggcaa agcctctcgg aaggaagtag tcgtttagtg tatagctaga
     8881 gttgatgtga gttagctttt cctcagcctc tgctataagc tcagctttac cttggggttg
     8941 gacgagattt agttgaggtt tctctagctc aatcttgtga ggaagtttaa cctcaagctc
     9001 gccctccatc ttgtagagag ccttgtcact agccttgtca ttggttccct gatgataagg
     9061 gctggctgtc atgatggcag ggatttttcc atcaaaacga gggcgaataa tgctaacctt
     9121 tactaggtct gatagccctt tttggtcagt atcgacacga gactcaacgt aaacgacttc
     9181 acgaatgaca tcctggttag aaaaagtagc caaactcttg ccgttaaagt agtggtagtc
     9241 attatcctcc ggaataagac catcactaac aagttggtcg ataagagtat ttcctttttt
     9301 ggtgcgagta ttgagtaact gatagagatt ttcaatcaag tcaccatata taatgggaaa
     9361 tccagtttct ttacgaaaaa cgtcactatc ttcgaagtca accaaataag aaaagcctaa
     9421 aagttgaaaa gcaacagtat aaaaaatatc tgctgtcagt tcatcttctg attgaaaaaa
     9481 tgtcagcagg tctgtttttt tatcagctgc taggatagaa agtgggtagt tggtgtcttg
     9541 ataagtgaaa aagaaacgac gtaaaaaggt ttcaagtgag tctttgtgat tggctgtatt
     9601 ttgtaaatca aagccacatt tttttagttc agataagaca ttttcttttg gaaaattgat
     9661 ataactatat tgattaaaac gcatagaacc tccatataga atgacagtta aggttattat
     9721 atcaaaaaaa aagcagaaag ggaattgtta acttcaaaag gaaataatcc aataaa
//
LOCUS       DAVKFH010000073         9758 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKFH010000073 DAVKFH010000000
VERSION     DAVKFH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.073"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(162..1925)
                     /locus_tag="VW006_001834"
     CDS             complement(162..1925)
                     /locus_tag="VW006_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9380639.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(1934..2524)
                     /locus_tag="VW006_001835"
     CDS             complement(1934..2524)
                     /locus_tag="VW006_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9380640.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VW006_001836"
     CDS             complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VW006_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9380641.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(3190..3882)
                     /locus_tag="VW006_001837"
     CDS             complement(3190..3882)
                     /locus_tag="VW006_001837"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9380642.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            complement(3899..4213)
                     /locus_tag="VW006_001838"
     CDS             complement(3899..4213)
                     /locus_tag="VW006_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9380643.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVINSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(4225..4770)
                     /locus_tag="VW006_001839"
     CDS             complement(4225..4770)
                     /locus_tag="VW006_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9380644.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VW006_001840"
     CDS             complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VW006_001840"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9380645.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            6303..7103
                     /locus_tag="VW006_001841"
     CDS             6303..7103
                     /locus_tag="VW006_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9380646.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            7198..8040
                     /locus_tag="VW006_001842"
     CDS             7198..8040
                     /locus_tag="VW006_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9380647.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            8040..8522
                     /locus_tag="VW006_001843"
     CDS             8040..8522
                     /locus_tag="VW006_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9380648.1"
                     /translation="MAKKVKIKKTLAEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            8522..9142
                     /locus_tag="VW006_001844"
     CDS             8522..9142
                     /locus_tag="VW006_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9380649.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(9396..9491)
                     /locus_tag="VW006_001845"
                     /pseudo
     CDS             complement(9396..9491)
                     /locus_tag="VW006_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2980 a         2075 c         1689 g         3014 t
ORIGIN      
        1 agaagttata gtgtagcatt ccaacttcaa agcactataa agtaaattga aacaagaaca
       61 atacaaacaa ttctcgtaaa cggattgcaa ccacaaaaaa gcaagcattc acaagaatac
      121 ttacctatca tgggaggaac aaccgttcct cttttttatt actaaaattc aaagaattcc
      181 aatgcttttt tcaagagcaa atccgtatat tctggatctt cttgggctac ttctatttcc
      241 cgctgaactt tttccaaatc atctgtaatc actccatcta ctcctaagtg aagagatttg
      301 ctgatagctt ctgaatcatt gacagtccag acataaagtt tctgatccgt tgtccatagt
      361 ttgcttacaa aatattcatc caaggttgag tactccatag tatatcctgt cgctcttgtt
      421 ttaggaaaga cagaattgta gggcatgatg aaataaactg gcagttcggc atcatactgt
      481 cttacttttt cgacaacatg gtagtctaaa gactggattt gatgtccata aatcttgagc
      541 tttgcagcat aacgggctaa aaagcggttc atcatgtctg gactatcttt tttactggtt
      601 ttaatttcaa ttagtaattt ttgaccaagt tcgttggctc gactgagata atcttcaaag
      661 cttgaaattt tagtctggta gccattttca aaaatatcaa tccctttaag ctcctccaag
      721 tttaagtctt gaggactttt attgatacct gctagatttt tcaagttagc atcatgcatc
      781 atgacaaact gcccatcttt tgtttcctgc acgtccgtct ccaccaagtc tggtttgagt
      841 tgtgctgtag tttccaagga ctctactgta ttttgaatcc catttgcatt ggaaacccct
      901 cggtgagaaa taagttgagg tagatgaacc atgggagcct ccagataaat ataaccttct
      961 aaggcaaaga aaaaactggc acaagtcatg acaccccatc gcacgatgtg atctttttct
     1021 ctcctaggaa gcatatccag ctcctttcct gtcaaaaatg aaacaaattt aaccaaaaaa
     1081 taagtcagag ccatataata gagattttta atcacgacaa aattcaaaat accaagaatc
     1141 agagactctc tctgagtgat atcatctacc aaagtttgag ccaataataa aggaatcaaa
     1201 ggaagataaa ataataaatg tgctttgagc aagatgtaaa ataaattcca agcataaaaa
     1261 gtaactctct tcttggtttt ctccaagcta aacatcactg cttctcgaac agtcagctga
     1321 tcatatacaa tcttcggaag ggcaaacatc aatctgacag agacatagag aaagataaga
     1381 gatagaagta ggatgctcag ccaccacatc caatatttat cttctaaata agcttggata
     1441 aactctggaa tgacgatttt attaagataa taaatcttca gcattttccg tataaaagga
     1501 aacagcatag ctatatagaa aaagataaac aaggctttag cgccagttag ctttttcata
     1561 aattcaaaac tttcatggaa aaccttgcgg atatactcaa ttagccttcg cttttcatta
     1621 tagaggagat gacgagcacc aataaagagg agtcctattt gaaaataagc aaccagaaag
     1681 ttaattacaa tcaaggctaa aaaagctaga ctaatcaatg gagaatgagt aaggatggct
     1741 aagacattgt tataggaaat aaaaagataa cctgtctgat ctaataagaa gctagccaac
     1801 catgaattga atggtaccca caaatactcc actatcataa aaatcaagaa aaatagaaag
     1861 aggattttat caagattgag gtaaatctgt ttaagaccca attttttagg tttttcaggt
     1921 ttcataggca ctcctagtca aataattgag acaagtccaa gccaccaaaa ggattgtttg
     1981 ataagctact ttctgtctct aacaattccc tagcttgatc cgactctaag aaggattcgt
     2041 aaacacgcgc cgtcatccga gcatcctcta aactattatg agactgacct tgaaatccaa
     2101 gaaatgaggc aacagtttgc aatttgagat tggcaatacc atgtaaatct gaactccgac
     2161 gttcaaaagc ttcatcatac aaatccacct tgtactgttg gctatagtct aaaccatgct
     2221 ctgctaaaat aggtaaatca cttttagcag cattgtagcc tatcatcagc aaagaaccca
     2281 caaactcttg gaattcttct aagactttct caactggagg agcatctttt aaggtctcag
     2341 ctgtaatccc ggtcaaacca ttgataaagc tttttagagg gacgctggta tgaacatagg
     2401 aatcataggc atcgatttcc cgaccatctc taaaacgcac tgccgatact tgaatcaagt
     2461 gtgtttgtcc ttcgtgctga ttaaattcta aatcaaaagc aatgtaatct tctaatcgtt
     2521 ccattctgta cctctcctct actgtctctg ttctaatcaa ctatactatt cacaaacaaa
     2581 agaagccatc aggttagcat ttaacctaaa cagctccttg attatcctcc aaataaacga
     2641 gcaaaaaagc cttttttagt ggattggact tcttcttttg cttggtccag ctctagctta
     2701 agattttctt gatccttcat ggcttgaagg gtcaattgtt gttgctgatc taattgtttg
     2761 tctttctcag caatctgacg gtctttgata cgcatctgct cgtctttttc tgataactga
     2821 cgatccttgg cttttaattg ttcataaaga cgaagaattt ctgcattctt ctcatccact
     2881 agaatctcca tcagttcacg ttgtttgaca tcttcactga caggttcatc ttcaaaaatc
     2941 gtttttttat agatttcttc tagcttaatc aagccacttc tggtaacaac tgttacccct
     3001 ttgtcatttt tatctgtgtc ttcttctggt aattctttga cacggttatt gattgcttgg
     3061 cgagataatc ctaagacctc tgcaatctca ctgacggtca tttcaatact cataatatcc
     3121 tctgaaacgt tttctagctt ttctttactt aaatcttatc ataagctaga aaaactgtca
     3181 aatttccgct taatctaaag ccttcaaaaa ggccaacaag acttgggcag agcgacgtcc
     3241 agcttcgata ataaactcat caaaaaagat gtttgcttca tggttggcat tgtcactcat
     3301 agctcggatg actaagactg ggagattgag ggtatgcgct gcttgagcaa tagctgcccc
     3361 ctccatctcc acggctaaaa cttctgggaa atgggactta atcgcttcta tcttgtcatt
     3421 tcctgcaaca aaactatctc ctgtagcaat caaaccaaga tgccagtttt ggtccaattg
     3481 agataaactc tcttggattt gagcaacaaa ggttttgtct gattcgaaat aaagcggttg
     3541 ttgcgccatt tgtccataag catagccaaa agctgtgaca tccacgtcat gataggctaa
     3601 tttgtcagca atcacgacat ccccaacagc gataccttct gctactgccc cagctgaacc
     3661 cgtattaata agggcatcca cctggaaatg atcagccaaa atcgccacac tcatagcaga
     3721 catgacctta ccaattccac tttctacaag aacgacttca tgagaagcaa tggttcctgt
     3781 atgataggta ttccccaaaa caacttgctc ctggacatta tataaatgct ggaccagata
     3841 agccagttct tctggcatag cagcaataat tcctattttc atttcaattc cttttctatt
     3901 acaaaagttt cattgctaaa acaagcaaaa tcaagagaaa gatgaccgcg aataagattt
     3961 tattcaactt agaattgatg acatttctct tggtattttc aatgcgacgg cttttatgaa
     4021 tagatggttc gacctgaatc ttcaaggttt cctggctaaa accatgattc ttaggattgg
     4081 cataaccaaa acgggaagaa gaggttggta aaatcttagt ctcctcatta tcatctagca
     4141 aaggaggacc tgaaattttt tcgcctctat tagctctttc aatcatttca tccgttaata
     4201 aagatttacc catactgacc tcctctattt tttctgcaac tcccaatact gaacagccat
     4261 aattgtcttg gcatcacaaa tatgacctga ttggattaat tctttcgctt cttctaagct
     4321 cacttcaagg acttccaagg tttcatcctc atcctgcgga cgcggatttt ccacttttgt
     4381 caaatcgctt gctaaatata gttttaactt ctcattacaa aagccaatag ctgaataaaa
     4441 atcgtacaag agttctaatt tccctgtata ggctgtttct tcctctaatt cacgaagggc
     4501 agctgccaca ggggctgtgt tttctcctac ttccaatttt ccggctggaa tttcgtaaga
     4561 gacagcctcg atagctttgc ggtactgctt gaccaagatc agtttttgtt catccgttac
     4621 tgctaaaaca cagacagccc cattgtggaa aatcaaatcc cgttgggcag ttcccttgcc
     4681 ttctggtaat tcaacctgat cttggaccag tttaaatatt ggtccttgat agatttcttt
     4741 tcggctaagc gttttttctt caaactccat gataggctcc tactggttct taggatgatg
     4801 aggaagacgt gttgcatatt cgtctttatt gatctgacga ccgcgaccaa tagcaatagc
     4861 atctgctggc acgtctttag taatagttga accagcacca acgagggaat tgtcaccaag
     4921 ttctactggt gcaataatgg ttgaatttga accaacaaag acattgtctc caatgactgt
     4981 cttgtatttg tttttgccgt cataattgac tgtaatagtt ccagcaccga aattaacgtt
     5041 gcttcccact tcacagtttc cgatataagt caaatgacca gccttggtat tctcaccgat
     5101 tgaagatcct ttcacctcaa caaagttacc aatatgaact tgggcaccca gacttgaatt
     5161 tggacgaatg tgagcataag gaccgactgt cacaccgtct gcaacactac tttcctcaat
     5221 catagaattg gtaatgaccg ctcctgctcc gatagtgctg tccactacat aagtaccgtt
     5281 tgtcaaaaca gtctcagcac caattttcgt ttgccctttc aaggtaacat tggcttcgat
     5341 ttgaacttcc ggagcaatct caacatcaat atcgatataa gttgcttctg gattgacaaa
     5401 gctaacaccg ttgaccatat gtttatgatt gatgcgacga cgcataactg actcagctgt
     5461 cgcaagcgcc acacggtcat ttaccccaag actttcatca aaatctttca aagtataagc
     5521 gccaactttt tcaccagttt cacggaaaat accaatgacg tctgtaatat agtattcgcc
     5581 ttgagcgtta ttggtattga tatttttcaa agcctcaaac aaacgctcgt tgtcaaagac
     5641 gtatgttcca gtgttgattt ccttgatttg cttttcaaaa tctgtagcat ccttctgctc
     5701 aacaatacga agaacctcag cattgtcatt acgaacaatt cgtccatagc caaaaggatt
     5761 atccgtttca gcagtcaaga tagtggccac atttttatga ttgatatgga aatcaatcaa
     5821 gtttttcaag ctttcaccag tgattaaagg agtatctcct gcaatgacca aggtgtgtcc
     5881 tgacaaacct tctaagatag gttctgtcat cataactgca tgaccagttc ccaactgttc
     5941 agattgagtc acaaattctg tctgtccagc caagacctcc tcaaccaatt ctgccttgtg
     6001 tcctacaact gttactgtct tttcaggttg gatagctccc acactacgga aaacatgttc
     6061 caacatagaa atacccgcaa ccttgtgcaa aacttttggc aaatcagatt tcatgcgagt
     6121 ccctttaccc gctgctaaaa taatggcaaa atttgacata atcttctctt ttctgtagaa
     6181 attccctttt attataccat atttcaaatc attccgcact cttgaatgaa aaaatgctct
     6241 aaagtctctt ccttaaccca tttttttgat tttttttgat ttttagggta gaattataag
     6301 atatgagaaa acaaattcat ccacttattt tatttagttt tttggggatt atgatgttca
     6361 ttttaatcat caatcgccca cttaatgaca accaatcgtt taaaacaaaa tccaatatag
     6421 cacagattga agcacaggct ttgaaacact tagacaaacc gattattgac ctctctggct
     6481 ggcagcgtcc tgaagaaatc aactacgatg ccctctcaca aaatatttca ggtgctattg
     6541 ttcgtgttca cagtggtgct caaacgacaa aagaaaatga tgcttcattt atcaatggaa
     6601 tagacaaggc ctataagagt catatcactg aacttcaaaa gcggaatgtc ccagttgctg
     6661 tctacgctta tgtagctgga aaaagtgtcc aagaaatgga aaaggctgcc gaagttttct
     6721 acaatgctgc ctctccttac agccctagtt actattggct tgacgtggaa gacaaaacca
     6781 tgtctaatat gaacgaaggt gtcgaaaact ttcgagcaaa actagcatca ctaggtgcta
     6841 aaaacatcgg catctacgtt ggtgtctact tcatggaaga acacagtatt gatacaggca
     6901 agtttacctc tgtttggatt ccgtcctacg gctcagactc aggatttttg gagagcagtc
     6961 ctaaaactga tttagattat gatattcacc aatacacatc taaaggaaaa attgccggct
     7021 ttgaccacga tttagatatc aacgttatct ctcccttaaa aaataaagaa gaaacctttc
     7081 gaaaactttt tttaaaacct taaaagcatt tgtttatcat ttgctttttc tttttgtgtc
     7141 tgattgtgat acaatatagt aaggattttt tgaaatagaa atagttggag gaaatatatg
     7201 ctctcatggt tagcacgcgt tattaaaggg attgtaattg ctcttggatt tatcctaccg
     7261 ggaatttccg gaggggttct agcagcaatc ttaggaatct atgaacgaat gattggcttt
     7321 ctggcccatc cctttaaaga ctttaaagaa aatgttttgt actttattcc agttgccatc
     7381 ggtatgcttc tgggaatcgg cttattttcc tacccgattg aatacctgct tgaaaattat
     7441 caggtttttg tattatggag ctttgcggga gctattatcg gtacagttcc tagcctcctc
     7501 aaagaatcaa ctcgagaatc tgaccgagac aagattgatt tagcttggtt atggacaacc
     7561 tttatcattt ctggattagg actctatgcc ttaaattttg tcgttggaac cttaagcgcc
     7621 agctttctta acttcgtcct agcaggcgca ctattggccc ttggcgtctt ggttcctggc
     7681 ctcagcccat caaatttact tttgattttg ggactctatg ctcctatgtt gactggtttt
     7741 aaaacttttg atttcttggg aaccttcttt ccgattggaa ttggtgcagg tgcaactctc
     7801 atcgtttttt caaaattgat ggattatgcc ttaaacaact accactcacg tgtctatcat
     7861 ttcatcatcg gtatcgtcct atcaagtacc cttttgatct taattccaaa tgcaggaaac
     7921 gctgaaagta tccaatacac aggactttca cttgtcggtt atgtcatcat cgccttcttc
     7981 tttgcgctgg gaatctggct tggtatttgg atgagtcaat tggaggataa atataaataa
     8041 tggcaaaaaa agttaaaatc aaaaaaacat tggcggaaca aatcctatct aaagcagcta
     8101 tccctcatca ggggattcaa atcaatgccc tagaaggaga gcttcctcaa ggttatgaac
     8161 gagatcagat tttcaaaacc ttggcgcttt tgggagacaa gaccggaccg attatcggaa
     8221 ttgtccctat cactcagcac ttgtctgaaa agaaactagc taaaatttct ggcaataaaa
     8281 aagtgagcat gattccacaa aaggacttag aaaaaacaac tggttacatt catggagcca
     8341 ataatcctgt cggaattcgt cagaaacaca attatcccat ttttatcgat aaaattgctc
     8401 tagatttgga tcaaatgatt gtctctgcag gcgaagtcgg acacagcatt atcgtcgcgc
     8461 cacaagactt ggctagcttt gtaaaggcag actttgtaga tattttggag gacatcaagt
     8521 aatgaaactc tactttgtcc gccacggtcg taccctctgg aaccaagaag gtcgttttca
     8581 aggtgctagt ggtgattctc ctcttcttcc tgaatccatt gaaaccctaa aacgacttgg
     8641 tcagtatctc aaggaaattc cttttgatca gatttattca agtgatttac ctcgagcggt
     8701 caaatctgct gagattatcc aaagtcaact ctataccccc tgttctttag aaatcgttcc
     8761 taatctccgt gaatggcaac ttgggaagtt agaaggtttg aaaattgcaa ccttggaagc
     8821 tatttacccg caacaaatcc aggcttttcg ttcaaatctt gctcaatttg acactcgaat
     8881 gttcggagcc gaatccctct atagtacaac tcaacggacc atccaattta tcaaatcatt
     8941 aaaagatagt ccagctgaac gtattctaat tgtcggacac ggcgccaatc ttactgccag
     9001 tcttcgtact ctcctaggct ataaagaacc acttcttcgt aaagatggcg gtctagcaaa
     9061 tgccagcctg accattatag aaacccatga ttttgaaaca ttcactctca atacttggaa
     9121 tgatacttct tatcaatcat aattatagcc tagccttctg aactagaacc ataggagata
     9181 tagtcagttg aaacaaaaat aagataaaag agcctcgtaa aaaggtattg caacttgcca
     9241 ataccttttt tgatatgaac acgtgtcttc tcaacaggat tgtagagcaa aatgaaataa
     9301 gaactgaata atatgatttg agaatttaaa tcaatttata agaatgtttt agaagcagtt
     9361 atgtactatt ttagattcac ttttctatac taagatgagt agggaggaaa aggtaaaagt
     9421 ttatgcccaa actcttcaca taagaactct agcttaccca ttctatggaa tcttgcatta
     9481 tccattaaaa cttccagact catacccttc taaaatctat tcaaaccacg tcagcttcac
     9541 attatatatg ttggttacct cacaagtttt atctgcaacc tcaaagctgt tctttgagca
     9601 gcctacagct agcttcctag tttaatcttt gcttttcact gagtatcaag ttaaaaaatg
     9661 aggctggtca aaaagtcttt aaaataaaaa aaacgcatat tatcaggtgt taaatgaact
     9721 gcaccccaaa agttagacag aaaaaatcta acttttgg
//
LOCUS       DAVKFH010000074         9725 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKFH010000074 DAVKFH010000000
VERSION     DAVKFH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.074"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            101..3202
                     /locus_tag="VW006_001846"
     CDS             101..3202
                     /locus_tag="VW006_001846"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9380650.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVSYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3285..4292
                     /gene="pfkA"
                     /locus_tag="VW006_001847"
     CDS             3285..4292
                     /gene="pfkA"
                     /locus_tag="VW006_001847"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9380651.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4351..5856
                     /gene="pyk"
                     /locus_tag="VW006_001848"
     CDS             4351..5856
                     /gene="pyk"
                     /locus_tag="VW006_001848"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9380652.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6591..6816)
                     /locus_tag="VW006_001849"
                     /pseudo
     CDS             complement(6591..6816)
                     /locus_tag="VW006_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7664..8536
                     /locus_tag="VW006_001850"
     CDS             7664..8536
                     /locus_tag="VW006_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9380653.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8682..9026)
                     /locus_tag="VW006_001851"
                     /pseudo
     CDS             complement(8682..9026)
                     /locus_tag="VW006_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9169..9481
                     /locus_tag="VW006_001852"
                     /pseudo
     CDS             9169..9481
                     /locus_tag="VW006_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9485..9725
                     /locus_tag="VW006_001853"
                     /pseudo
     CDS             9485..9725
                     /locus_tag="VW006_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT         3007 a         1687 c         2070 g         2961 t
ORIGIN      
        1 tagaaaataa attagtaagc tgatgaaatt tttctcaaga gaagccattt ataggtgaaa
       61 atggtataat atagtgagaa ggatagagga gaagtgtaaa ttgatcgcac aactagatac
      121 aaaaacagtc tatagtttta tggaaagcgt catttcgatc gaaaagtatg tgagagcagc
      181 taaagaatac ggctacactc atttggctat gatggatatt gacaatcttt atggcgcttt
      241 cgactttcta gagattacaa aaaaatacgg cattcatcct ttgctagggc ttgaaatgac
      301 agtgtttgta gatgatcagg aagtgaattt gcgcttttta gctctatcta gtgtgggcta
      361 tcagcagttg atgaagcttt cgacagccaa gatgcagggg gagaaaactt ggtcagtcct
      421 gtcccagtac ctggaggata tcgcggtcat tgtgtcttat tttgatagag ttgagtcgtt
      481 agaactaggc tgtgattact atataggggt ttatccagaa acactagcaa gcgaatttca
      541 tcatcctatc ttacctcttt atcgggtcaa cgcttttgaa agcagggata gagaagttct
      601 tcaagtttta acagcgatta aagaaaatct accgctcaga gaagttccct tgcgttcgag
      661 acaagatgtc tttatatcag caagttcttt agagaaacta ttccaagagc gttttccgca
      721 agctttggac aatttagaaa agcttatttc aggcatttct tacgacttgg atactagtct
      781 gaaactgcct cgttttaatc cagctagacc agcagtagag gagttgagag agcgtgctga
      841 actggggctt gttcagaagg ggttgactag taaagaatat caagatagac tagaccaaga
      901 attgtctgtt attcatgata tgggctttga tgattatttc ttggttgttt gggatttgtt
      961 gcgttttgga cgatcgaatg gctattatat gggaatggga aggggttctg cagtaggcag
     1021 tttggtttct tatgccttag acatcacggg gattgaccca gtagagaaaa atctgatttt
     1081 tgaacgcttt cttaatcgtg aacgctatac catgcctgat attgatattg atatcccaga
     1141 tatttatcgt ccagatttta tcagatatgt tggtaataaa tatggtagta aacatgcggc
     1201 acaaatcgtt actttttcaa cctttggagc caagcaagct cttcgagatg tcttgaaacg
     1261 ctttggtgtg ccagagtatg aattatctgc aattactaag aaaatcagtt ttcgtgacaa
     1321 tcttaagtcg gcctatgagg gcaatctcca gtttcgtcag caaatcaata gtaagttaga
     1381 ataccaaaaa gcttttgaga ttgcttgcaa gatagagggc tatccaaggc aaacctctgt
     1441 ccatgcggct ggtgttgtaa ttagtgacca agatttaacc aactacattc ctctaaagta
     1501 tggtgatgaa attccactga ctcagtatga tgctcatgga gttgaggcta gcggactttt
     1561 gaagatggac tttctgggac tacgaaattt gacctttgtc cagaagatgc aagagttgct
     1621 tgctgaaaca gaaggtattc atctgaaaat tgaagaaatc gatttagaag acaaagaaac
     1681 gttagcttta tttgcctctg gtaatacaaa aggtatcttt caatttgagc aaccaggtgc
     1741 cattcgtctg cttaagcgtg tgcaaccagt ctgttttgaa gatgtcgtcg cgactacttc
     1801 tctaaatcga ccaggtgcaa gtgactacat caataatttt gtggcaagga agcatgggca
     1861 ggaagaagtg actgttctag attcagctct ggaggatatt ttggctccaa cttatggcat
     1921 tatgctctat caggagcagg ttatgcaggt tgctcagcga tttgctggat ttagtcttgg
     1981 gaaggccgat attttgcgtc gagctatggg taaaaaggat gcctctgcta tgcatgagat
     2041 gagggcttcc tttattcaag gctccataga agcaggccat actgcggaaa aagcagagca
     2101 ggtttttgat gttatggaga agtttgcagg ttatggattt aatagatctc atgcctacgc
     2161 ctactcagcc ttggccttcc agttggctta tttcaaaacg cattatccag ccatttttta
     2221 tcaggtcatg ttaaattatt ccaacagtga ttacttaata gatgcacttg aagcaggttt
     2281 tgaagtagcc tctctatcca tcaatactat tccctatcac gataaaattg ccaacaagtc
     2341 tatctatata ggtttgaaat cgattaaggg gctcagcaag gacttggcgc tttggattat
     2401 tgaacataga ccttattcta acattgaaga ttttatagct aaattacctg agaattatct
     2461 gaaacttcct ctgctagaac ctttggtaaa agttggtctt ttcgattcat ttgaaaaaaa
     2521 tcgtcaaaaa gtatttaata acttagctaa tctatttgaa tttgtgaaag agttgggaag
     2581 tttgtttgga gatgctattt atagttggca ggaatcggaa gattggacgg aacaagaaaa
     2641 attttatatg gaacaagagc ttttagggat aggtgtcagc aaacatccac tacaagctat
     2701 tgcaagtaag gctatttacc cgattacccc aatcggaaat ttgtcagaaa atagctatgc
     2761 tattattttg gttgaagttc agaaaataaa agtgattcgt accaaaaagg gtgaaaatat
     2821 ggccttctta caggcagatg atagtaagaa aaaattggat gtcactctct tttcagactt
     2881 atatcgtcag gttggacagg aaataaaaga gggagccttc tactatgtaa aaggaaaaat
     2941 acaatcacgt gatggccgtc tgcaaatgat tgcacaagaa ataagagaag cagttgctga
     3001 acgcttttgg atacaggtga aaaatcatga atcggatcaa gaaatttcac gcattttaga
     3061 acaatttaaa ggcccaatcc cagtcatcat ccggtatgaa gaggaacaga aaaccatcgt
     3121 ttctccccat cattttgtag ctaaatccaa tgaattagag gagaaattga atgaaatcgt
     3181 tatgaaaacg atttatcgct aaaaatacgg aaaatagaag aattttaaaa tcaaatgtga
     3241 tataatcaag aagaatgtta aaagaaaaag gagcataaac caatatgaaa cgtattgctg
     3301 ttttgactag tggcggagac gcacctggta tgaacgctgc catccgtgca gttgttcgtc
     3361 aagcaatttc agaaggaatg gaagtgtttg gtatctatga cggatatgct ggtatggttg
     3421 ccggtgaaat tcatccccta gatgcagctt cagtagggga catcatttct cgtggtggta
     3481 cttttcttca ctcagctcgt tatccagagt tcgctcaact tgaagggcaa cttaaaggga
     3541 ttgagcaatt gaaaaaacac ggaattgaag gtgtagttgt tatcggtggt gacggatctt
     3601 accacggcgc tatgcgtttg actgaacatg gcttcccagc tattggtctt ccaggtacaa
     3661 tcgataacga tatcgttggt actgacttta caatcggttt tgacacagcg gttactactg
     3721 ccatggacgc tatcgataag attcgtgata catcatcaag tcaccgtcgt acttttgtaa
     3781 tcgaagttat gggacgtaac gctggtgata tcgctctttg ggctggtatt gcaactggtg
     3841 ctgatgaaat catcatccct gaagcaggct tcaagatgga agatatcgta gcaagcatca
     3901 aagctggtta tgaatgtggt aaaaaacaca atatcatcgt cttagctgaa ggtgtgatgt
     3961 cagcggctga atttggtcaa aaacttaaag aagctggaga tacaagcgac cttcgtgtaa
     4021 cagaacttgg acatattcaa cgtggtggtt ctccaactgc gcgtgaccgt gttttggcgt
     4081 cacgtatggg tgcacatgct gttaaacttc ttaaagaagg tatcggtggt gttgcggttg
     4141 gtattcgtaa cgaaaaaatg gttgaaaatc caattcttgg tactgcagaa gaaggggcat
     4201 tgtttagcct tactgcagaa ggtaagattg tggttaacaa cccgcacaaa gccgatattg
     4261 agctatctag cttgaataag agcttgtcat aatttacaat ttagttaata agaccaaaaa
     4321 ggtcatatat ataaaggagt cacaaaaatc atgaacaaac gtgtaaagat cgttgcaact
     4381 ttgggtcctg cggtagaaat ccgtggtggt aaaaaattcg gtgaggacgg atactggggt
     4441 gaaaaacttg atgttgaagc ttcagctaaa aacattgcta aattgattga agctggtgct
     4501 aacacattcc gattcaactt ctcacacggc gaccaccaag aacaaggtga gcgtatggca
     4561 actgttaaac ttgcggaaaa aattgcaggt aaaaaagttg gtttccttct tgatacaaaa
     4621 ggacctgaaa tccgtacaga attgttcgaa ggtgaagcta aagaatattc atacaaaact
     4681 ggtgaaaaaa ttcgtgttgc aactaaacaa ggaatcaaat caactcgtga agtgattgcg
     4741 ttgaacgttg ctggtgctct tgatatctat gatgatgttg aagttggtcg tcaagttttg
     4801 gttgacgatg gtaaacttgg tcttcgtgtg gttgctaaag atgatgcaac tcgtgaattt
     4861 gaagttgaag ttgaaaacga tggtatcatc gctaaacaaa aaggtgtgaa catccctaac
     4921 actaaaattc ctttcccagc tcttgctgaa cgcgataacg acgatatccg tttcggtctt
     4981 gaacaaggta tcaacttcat cgcaatttca ttcgtacgta ctgcaaaaga tgtgaacgaa
     5041 gttcgtgcaa tctgtgaaga aactggaaac ggacatgttc aattgttcgc taaaatcgaa
     5101 aaccaacaag gtatcgataa cttagatgaa atcatcgaag cagctgatgg tattatgatt
     5161 gctcgtggtg atatgggtat cgaagtaccg ttcgaaatgg ttccagttta tcaaaaaatg
     5221 attatcaaga aagtcaatgc tgcaggtaaa gttgttatca ctgcaacaaa catgcttgaa
     5281 acaatgactg aaaaaccacg tgcaactcgt tcagaagtat cagacgtatt caacgctgtt
     5341 atcgacggaa ctgacgctac aatgttgtca ggcgagtctg caaacggtaa atacccactc
     5401 gagtcagtaa ctacaatggc tacaatcgac aagaacgctc aagctcttct taatgaatac
     5461 ggacgtcttg attcagattc atttgagcgt aactctaaga cagaagtaat ggcttctgct
     5521 gttaaagatg ctactagctc aatggatatc aaattggttg taactcttac taagacaggt
     5581 catactgcac gtttgatttc taaataccgt ccaaatgctg acatcttagc attgacattt
     5641 gacgaattga cagaacgtgg cttgatgttg aactggggtg ttatcccaat gttgacagat
     5701 gctccatctt caactgacga tatgttcgaa atcgctgaac gtaaagcggt agaagcaggt
     5761 ctcgttgagt caggcgatga tatcgttatc gttgctggtg tgccagtagg agaagctgtt
     5821 cgcacaaaca caatgcgtat ccgcacagta cgttaagaaa aatataaaaa cctatcatat
     5881 ccagctttct agcctgtatg atgggctctt tttttgtcca gggggcaaaa agggggcaaa
     5941 actttcataa tggattgata ctcttcgaaa atcaaattca aaccacgtca gcgtcgcctt
     6001 accgtatata tgttactgac ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag
     6061 caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatga aataagatat
     6121 gcataaattg attagaaagt caaatgaatt tctacaaatg ttttagcaat cgcaatctac
     6181 ttgtctagat tcgatctgat atattttcga tttaatgata tggtatttaa aacctccaaa
     6241 gtagcttgct ccattctttt acctacgtga gtctagatgt tatttactgt tttagcgttt
     6301 ttgtgttcta ctctaaccat tatagcattc ttctcagcta gtgtactaag gagtgtgtgc
     6361 ctgaaaatat gggaactaag gggctggttt atcggtttct ctagtttagt atttgccttt
     6421 tgcaaagtga tcttaaatgc ctttctctaa atttacatat cactattgtt taacaaaatc
     6481 taatctattt taggtcactt attctttttt tgaaatgtag aatgaacttt ttcaaagttt
     6541 ttcgaatctt ttaaaatctg tttgttttat atcgccattc tccccctttt ttaattctcc
     6601 ctatatagcc tgacaacttt tccgatggta cgaatatggt tgctttcgtc taggtggatg
     6661 tcggagtatt cgggattgag tttttttgag gtagccttgg cggagtttct tgacatagtt
     6721 agtgccgtct acttggaaga tgccgatggt attatagtca atctgtgggg tattcttgat
     6781 aaataggtag tcgctgtttc ttatctttgg ctccatggac ttgctgacga cataagcgat
     6841 tgggtcgtag tcgtctggga taatggaaac tccataacta aatcgtcgtc ctgcatcaag
     6901 cggctacctg cagagataaa ctacctaaca cgagagtaag tagtctgtct gtagtcgtcc
     6961 agtctgatga tttttacgat acttcgtttt tctgatcata cagttgcctc tcggcatagg
     7021 tcagaacttt accttgtctg ggtggtgccc gttggtcgta gatagattgg atatcgctag
     7081 gagaatcctt ttgaactgga ggaaagaggg catcaatcaa gctactgaat actttaacta
     7141 agtcaaatat agtatttttc ttagtagacc taaccctttt ttcataattt ctaatggtgt
     7201 ttttacttat acctatctta gtacccaatt cttattgagt ccaaccatta ctagtctata
     7261 ttgttttata gttgattgag tttggaatag tacgctgtag ctgctaaaac atttctagga
     7321 attaatttgc ctttcctaat agagttgttc atatcttatt tcaatctact atatttttca
     7381 cctctaacaa tcgcaatctc ttctttatcc atgaacgaaa acgctttcta tctttgtaag
     7441 taaagcatca cacgaaatcc acgaaaatga aaacctttgt tgtgttttcg taaaaaattt
     7501 gttgacagag cacgaaacgc ctactataat taaatcaagt ttaaggaaat aacaaaaaaa
     7561 caaatcggag ggaaagacga tgaacacatt aaatgagaaa gtaatcaata tctgtaaagc
     7621 agtagttaaa gaaactttaa tccaagacat ttaggagaaa gaaatgaaga aatcaagaaa
     7681 attagccaca ttaggaatct gttcagcctt gtttttaggc ttggctgctt gtcaacaaca
     7741 acatgctact tctgagggga cgaatcaaag gcaaagcagt tcagcgaaag ttccatggaa
     7801 agcttcatac accaacctaa acaaccaggt aagtacagaa gaggtcaaat ctctcttatc
     7861 agctcacttg gatccaaata gtgttgatgc attttttaat ctcgttaatg actataatac
     7921 cattgtcggc tcaactggct tatcaggaga tttcacttcc tttactcaca ccgaatacga
     7981 tgttgagaaa atcagtcatc tctggaatca aaagaagggc gatttcgttg ggaccaactg
     8041 ccgtatcaat agttattgtc ttttgaaaaa ttcagtcacc attccaaagc ttgaaaagaa
     8101 tgaccagttg cttttcctag ataatgatgc gattgataaa ggaaaggtct ttgattcaca
     8161 agataaggaa gagtttgata ttctattttc gagagttcca actgaggcaa ctacagatgt
     8221 caaggttcac gctgaaaaga tggaaacatt cttctcacaa tttcaattca atgaaaaagc
     8281 tcgaatgctg tctgtagtct tgcacgacaa tctggatggc gagtatctgt ttgtaggcca
     8341 cgttggggtc ttagtacctg ctgatgacgg tttcttattt gtagagaaat tgactttcga
     8401 agagccctac caagcgatta aatttgctag taaggaagat tgctacaagt atttaggcac
     8461 caagtatgcg gattatacag gcgagggact ggctaagcct tttatcatgg ataatgataa
     8521 gtgggttaaa ctttaatagt gttatgatat gacagtaatc ttaggatttt tatttctatt
     8581 tttgatgagt tggtgggtta tttcaatcgt taatagccag aaaccacgca aaaatgaaac
     8641 atttattggc tatatccaaa gatacgatat tgatgggaat ggctatcaat cattaccgtg
     8701 tcctcagaac tgagaggagt tcttgaaatc gtaaaactac tttgaacaag agttacttca
     8761 acccattggc tccgacggat taagttgctt tcgtgaacac caaaatcagc cgcaatttct
     8821 tcataagtgc ggtattctcg cacatattga agagtggcca taagaaggtc ttctaggctt
     8881 aatttaggtt ttcgtccccc ttttgtgtgt ttaagttgat aagcagtttt taatacagct
     8941 aacatctctt caaaagtggt gcgctgaaca ccaacaagac atttaaatcg tgcatcagtt
     9001 agttgcttac ttgcttcata attcatagaa ctactatacc atattttgtt tcgcaggaag
     9061 tctaatgcta aaatcttatc agtgagaaga tttcttaaag tttattcttt gtagtttcat
     9121 ctgtattagt acataattta acttagagaa aataggagga ctaatcgaat ggaattaaaa
     9181 gattttacag aaaaagaaca ggaaatgatt aagaaaaggc ttacaatgtc taatattagt
     9241 gacaaagaaa ctactgagaa gattctggcg ctagtaccac aagacttgat taagcgaatc
     9301 ccgttttttg tcagaaaaca tgctacaaca cgtacgatta aacgcatttc aattgaacac
     9361 cctgaactct acgctgtagc tcaaacaagt ggtgagattc cagaaaaaaa aacgcgaaga
     9421 attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata gtattaagta
     9481 gagaatgaaa aaatatttta ttggcggttt gggaagcaat gcctatcata gcaaggattt
     9541 tctttaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc atcttcgaga
     9601 tgaaacagaa ttgaaatcat ggtttaaaaa tgagattgta gaggaagaat ctatctgtct
     9661 gataggtcat tctattggag gcgatttagc tcgttatttc gcatcggaat ttgaagaagt
     9721 aaaga
//
LOCUS       DAVKFH010000075         9307 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKFH010000075 DAVKFH010000000
VERSION     DAVKFH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.075"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..352
                     /locus_tag="VW006_001854"
     CDS             <1..352
                     /locus_tag="VW006_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9380654.1"
                     /translation="VSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQ
                     VQTPRKSSKLKPLTVEDKTYNHALSKERSKVENIFAKVKTFKMISTTYRNHRKRFGLR
                     MNLIAGIINHELGF"
     gene            401..865
                     /locus_tag="VW006_001855"
     CDS             401..865
                     /locus_tag="VW006_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9380655.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            1022..1447
                     /gene="lacA"
                     /locus_tag="VW006_001856"
     CDS             1022..1447
                     /gene="lacA"
                     /locus_tag="VW006_001856"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9380656.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1478..1993
                     /gene="lacB"
                     /locus_tag="VW006_001857"
     CDS             1478..1993
                     /gene="lacB"
                     /locus_tag="VW006_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9380657.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            2004..2933
                     /locus_tag="VW006_001858"
     CDS             2004..2933
                     /locus_tag="VW006_001858"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9380658.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2935..3915
                     /gene="lacD"
                     /locus_tag="VW006_001859"
     CDS             2935..3915
                     /gene="lacD"
                     /locus_tag="VW006_001859"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9380659.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            4149..4985
                     /locus_tag="VW006_001860"
     CDS             4149..4985
                     /locus_tag="VW006_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9380660.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            5021..5338
                     /locus_tag="VW006_001861"
     CDS             5021..5338
                     /locus_tag="VW006_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9380661.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5340..7034
                     /locus_tag="VW006_001862"
     CDS             5340..7034
                     /locus_tag="VW006_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9380662.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            7249..8655
                     /gene="lacG"
                     /locus_tag="VW006_001863"
     CDS             7249..8655
                     /gene="lacG"
                     /locus_tag="VW006_001863"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9380663.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8745..8957
                     /locus_tag="VW006_001864"
     CDS             8745..8957
                     /locus_tag="VW006_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9380664.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         3001 a         1570 c         1905 g         2831 t
ORIGIN      
        1 tgtttctttg gatatcactg tgaactattg tcatgatatg aagttgttca aaatgagtcg
       61 cagaaatatc ggacaagctg gtaaaatctt ggctgacagt ggttatcaag ggctcatgaa
      121 gatatatcct caagtacaaa ctccacgtaa atccagcaaa ctcaagccgc taacagttga
      181 agataaaacc tataaccatg cgctatctaa ggagagaagc aaggttgaga acatctttgc
      241 caaagtaaaa acgtttaaaa tgatttcaac aacctatcga aatcatcgta aacgcttcgg
      301 attacgaatg aatttgattg ctggcattat caatcatgaa ctaggattct agttttgcag
      361 gaagtctaat aaagaaacta gcaaggtcag gccttacttt attggtggac atccaggatt
      421 taattgtcct ctacttgatg atgaagtcta tgaagattat tatctagagt ttgagaaaga
      481 agagacttgc tctgttccac gtcctttccc agaaacaggt atgttggatt tccaagatag
      541 aagtccatgg ctagagggac aaaaagaaat agatcttagt tatgatcttt tcagcaaaga
      601 tgcagtgact ttggatgaat tgcaatctcg aacaattgcc cttcgttctc ttaaacatga
      661 taggggattg aaagtgcatt ttgcagaatt tccaaacctc atcatctggt caactttgaa
      721 caagggacct ttcattacct ttgaaccatg gtctggcttg tcaacattcc ttgaagaagg
      781 agatcattta gaagataaga agaatgtttg tctcttagaa gccaatcagg ttgaagaatt
      841 agggtttgag atagaagttt tataaaaaat taaaaaataa ataacaaaaa caaacacaaa
      901 ctgttgactt cttcaaacaa tagtagtata ttatgtttgt gaggactaat tgactgtttt
      961 ttaaagacag ccaatacact gctggaatcc agcatagaaa aaataaaaag gagtatacaa
     1021 tatgtctatt gttatcggtg cagatgctgc aggtttgaga ttgaaagaag ttgtgaaaga
     1081 cttcttagaa aaagaaaact tccaccttgt ggatgttaca gctgaaggtc aagactttgt
     1141 tgatgtgact ctcgctgttg ctgcagaagt aaacaaagaa gaacaaaacc ttggtatcgt
     1201 gattgatgct tatggagctg gtccatttat ggttgcaact aagatcaaag gaatggttgc
     1261 tgcagaagtt tctgatgaac gttcagctta tatgactcgt ggccacaaca actcacgtat
     1321 gatcactatg ggtgcacaac ttgttggtga tgaattggct aaaaacatcg ctaaaggatt
     1381 tgttaatggt aaatacgacg gtggtcgtca ccaaatccgg gttgacatgt tgaacaaaat
     1441 gggctaatta gattatagta agaaaggtaa gttaaaaatg agaattgcaa ttggatgtga
     1501 ccacatcgta acagatgaaa aaatggcggt ttcagaattt ttgaaatcaa aaggatatga
     1561 agtcattgac tttggtacat atgaccatac acgtactcac tacccaatct ttggtaaaaa
     1621 agtaggggaa gctgtaacta gcggtcaagc tgatcttgga gtatgtatct gtggtactgg
     1681 tgttggtatc aacaacgctg taaataaagt tccaggtgtt cgttctgcct tggttcgtga
     1741 tatgacaaca gccctttatg ctaaagaaca attgaacgct aacgttattg gttttggtgg
     1801 taaaattact ggtgaattgc ttatgtgtga tatcatcgaa gctttcatcc atgctgaata
     1861 caaaccaact gaagaaaaca aaaaattgat tgcgaaaatt gaacatgttg aaagtcacaa
     1921 tgctcaacaa acagacgcaa acttcttcac tgaattcctt gaaaaatggg atcgcggaga
     1981 ataccacgac taagaggtaa cctatgattt taacagttac aatgaaccca tccattgata
     2041 tttcctatcc cttggatgag ttgaagattg atactgtcaa tcgtgtggtg gatgtaacca
     2101 aaacggctgg tggtaaggga ctcaatgtta cccgagtact ttcagaattt ggcgattctg
     2161 ttcttgctac tggtttagtg ggtggcaaac ttggtgagtt tttggttgaa catatcgata
     2221 atcaagtaaa gaaagatttc ttctcaattc agggagaaac tcgtaactgt atcgctattc
     2281 tccacggaga caaccaaaca gaagttcttg aaaaaggtcc tgaagtattg gaacaggaag
     2341 gtcaagactt tttggaacat ttcaaaaaac tcttggagtc agttgaagta gtggccatct
     2401 caggtagtct gccagctggc cttccagttg attactatgc gagcttggta gaacttgcta
     2461 atcaagctgg caagcctgta gtcttggact gctcaggtgc agcacttcag gctgttcttg
     2521 aatcacccca taaaccaaca gtcatcaaac caaataatga agaattgtct cagcttcttg
     2581 gaagagaagt ttctgaggat ttggatgaat taaaagaagt acttcaagaa cctttgtttg
     2641 cagggattga atggattatc gtttcacttg gtgccaacgg tacttttgcc aaacatggtg
     2701 acactttcta caaggtagat attcctagaa ttcaggtggt aaatcctgtt ggatctggag
     2761 actctactgt ggcaggaatt tcttcaggac ttcttcacaa agaatcggat gcagaattac
     2821 tcatcaaggc aaatgtcctt ggtatgctca atgctcaaga aaaaatgact ggtcatgtca
     2881 acatggccaa ctatcaagtt ctatatgatc aattaatagt aaaagaggta taaaatggct
     2941 ttaacagaac aaaaacgtgt acgcttagaa aaactttctg atgaaaatgg tatcatctca
     3001 gctcttgcat ttgaccaacg tggtgctttg aaacgcctca tggctcaaca ccaaacagaa
     3061 gaaccaactg tggcccaaat ggaagaactt aaagtcttgg tagcagatga attgactaaa
     3121 tacgcttcat ctatgcttct tgaccctgag tatggacttc cagcaactaa agctcttgat
     3181 gaaaaagctg gtcttctcct tgcttatgaa aaaacaggtt atgacacaac aagcacaaaa
     3241 cgcttgccag actgcttgga tgtctggtct gcaaaacgta ttaaagaaga aggtgcagat
     3301 gcagttaaat tcttgcttta ctatgatgta gatagctcag acgaactcaa tcaagaaaaa
     3361 caagcctaca tcgaacgcat cggttctgag tgtgtggctg aagatatccc attcttcctt
     3421 gaaattcttg cttacgatga aaaaattgcg gatgcaggtt ctgtagaata cgcgaaagta
     3481 aaaccacaca aagttatcgg cgctatgaaa gtcttttcag acccacgctt taacattgat
     3541 gtcttgaaag ttgaagttcc tgttaacatt aaatatgttg aaggcttcgc tgaaggtgaa
     3601 gtagtttata cacgtgaaga agcagcagcc ttcttcaaag cgcaagatga agcaacgaac
     3661 ttgccataca tctacttgag tgctggtgta tcagctaaac tcttccaaga tactcttgta
     3721 tttgctcatg aatcaggtgc aaactttaac ggagttcttt gtggccgtgc aacatgggct
     3781 ggatcagttg aagcatacat caaagatggt gaaacagcag ctcgcgaatg gcttcgcaca
     3841 actggatttg aaaacattga tgagctcaac aaagttcttc aaacaacagc aacttcatgg
     3901 aaagaacgtg tgtaagaaag tcctcctagt ttaggaacat gaatctaaaa aaatttaaaa
     3961 aaagttgtat gtaaaggttt acaaaataac ttacttgtgc tatacttaaa tcacaagtta
     4021 atacaaggtg agtgttacta agtaatatta ggcatgatca caggtgaatt agaaatcagc
     4081 tgattttcta gttcatttgt ggtcattttt tgtactcata tacctttaag atataaaagg
     4141 aggttgacat gtatcgaatt ctaaatccaa tgaatcacaa tgtctcgctt gtcagaaatg
     4201 ataagggaga agaggtgatt gtaattggta aggggattgc attcggaaag aagaaggggg
     4261 atttgattgc tgaaaatcat gttgagaaaa tctttcggat gaagaccgaa gagtccagag
     4321 aaaactttat ggctcttctc aaagatgttc cgcttgattt tatcacagtg acctatgaaa
     4381 tcattgataa gctatcaaag aaatatcatt atccgattca agagtatctc tatgtaacct
     4441 tgacagatca tatttactgt tcttatcaag ctctaactca aggaaggtac aaggatagta
     4501 atctgccaga tatttctgct aagtatcctg tcgcttttca aatcgcaaat gaagcttttg
     4561 aaatttactg tcagaagcta gcagatcatt ttcctgagga cgaaattatt cggattgctt
     4621 atcatttcat taatgctgaa ggtgaaaatg aagtggaact tgtggagtcg attgataaga
     4681 ggaaagaaat tctcaggaat gttgaagaag ttttaacgga ctatgcaatt caacgaacta
     4741 aaaagaataa ccatttctat gatcgcttta tgatccattt gaattatttc ttggattatt
     4801 tagacagatc tagagatgat aaccaatcac ttctggatat ggaagatcat attaaacaat
     4861 cctatccaaa agccttcgag attggttcca agatctatga tgtgattacg caacatacgg
     4921 gtcttgattt gtataaaagt gaacgagttt atctagttct acatatccaa cgtttattgt
     4981 cataaaaatt tatttaaaac tatataagga gaattctatc atgaatagag aagaagtaac
     5041 attgttaggt tttgaaatcg tagcctatgc tggcgatgct cgttcaaaac tattggaagc
     5101 cttgaaggct gctgaagctg gtgattttga aaaagcggac gctctggtag aggaagctgg
     5161 tagctgtatt gcagaggctc accacgcgca aacaagtcta ttgactaagg aagcttctgg
     5221 tgaggactta gcgtatagtg tgaccatgat gcatggtcaa gatcacttga tgacaactat
     5281 cttgttaaaa gacctaatgc accatttaat tgaactttac aagagaggag ttaaataaaa
     5341 tggataagtt aataattttt attgagaaag gaaagccttt ctttgagaaa ttatcgagaa
     5401 atatatactt aagagcgata aaagatggtt ttatttccag tatgccagca gtattattct
     5461 caagtatttt tatactaata gctgctgtgc ccaatatttt tgggtttaaa tggtctgatg
     5521 aacagttagc ttttattttg aaaccttaca actactcaat ggggatttta gctctcttag
     5581 tagctgggac aactgcaaaa tcgttaacag attcagttaa tacacgtagt atggagaaaa
     5641 ccaaccaaat caactacatg tcaacttttt tagcagctgt tgttggattg ttaatactgg
     5701 cagctgatcc aattgaaggt ggatttgcta atggtctttt aggtactaga ggattactta
     5761 ctgcattttt ggcagctttt attacagtaa atatctataa agtatgtatt aaaaataatg
     5821 taaccattag acttcctgaa gaggttccac caaatattgc acaagttttt aaagatgtta
     5881 ttccgtttgc cttatctgtt ctttcaatat atggattgga tttaattgtt agaaatattt
     5941 ttggtacaaa tgtagcagaa tctgttggta aaatattagc ccctctattt tcagctactg
     6001 atggttatat cggtttagct attgtatttg gtgcttatgc tttcttttgg tttgttggaa
     6061 ttcatgggcc atctgttgta gaacctttaa ttgtagctat cagttatgca aatatagaag
     6121 ctaatgttca gcttgtgcaa gctggtatgc acgcagataa aattttaaat ccagttactc
     6181 aaacttttgt tgtaacaatg ggtggtactg gtgcaacact agtagttcca tttatgttta
     6241 tgtggttatg taaatcaaaa agaaatagaa ttgtcggacg tgcgtctgta gttcctactt
     6301 tctttggcgt aaatgaacca atcctatttg gtgctccaat tgttttaaat cctatcttct
     6361 ttattccgtt tgttactgct cctattatca atgtttggat tatgaaattt tttgtagatg
     6421 ttctacaaat gaatagtttc agtattattt taccttggac aacacctgct ccaattggta
     6481 ttgttatggg aacggcgctg gctccattgt cattcgtctt ggctataact ttaatcatta
     6541 tcgacacttt aatctattat ccatttgtta aagtttatga ccatcaaatt ttagaagaag
     6601 aacggaaagg aaactcttca tctgaattga aagaaaaagt tgctgcaaac ttcaacactg
     6661 caaaagcgga tgctattctt gaaaaagcag gtgtcgatgc agcacaaaat accatcactg
     6721 aagaaacaaa tgtcctcgtt ctctgtgctg gtggaggtac aagtggactc cttgcaaatg
     6781 ctttgaataa ggcagcagca gaatacaatg tccctgtgaa agcagcagca ggcggctatg
     6841 gtgctcaccg tgaaatgtta ccagagtttg atttggttat ccttgctcct caagttgctt
     6901 caaactttga agatatgaag gcagaaacag ataagctcgg tattaaacta gcgaaaacag
     6961 aaggcgctca atacatcaaa ttaactcgtg atggaaaagg tgccctagca ttcgtacaag
     7021 aacaattcga ttaaggctag gaactgtgaa acagtctcct atcgttacgg aaatcgctat
     7081 ggcgaatttc ctaatcgcct tattaattcg tcggtaaaaa gatatcgttt ttacctcctc
     7141 atgtcacaat tcggtgactt ggtacaagaa gtgagatgga gaaggatggc tcactgactc
     7201 ctctcctctc acttttactt tatttaaatc aagaaatagg tgaaaaaaat gacaaaaaca
     7261 cttccaaaag actttatttt tggtggcgca acagctgcct atcaagcaga aggtgctaca
     7321 catactgatg gtaaaggacc agtagcgtgg gataaatatc ttgaagataa ctactggtac
     7381 actgcagaac cagccagtga tttttacaat cgatatccag ttgatctcaa gctagcagaa
     7441 gagtatggtg tcaatggcat tcgaatttct attgcctggt cacgtatttt cccgactggt
     7501 tatggtcaag taaatgctaa aggtgttgag ttttatcata atttatttgc agagtgtcac
     7561 aaacgtcatg ttgagccctt tgtaactctt catcactttg acacgccaga agctctccac
     7621 tcaaatggag acttcttaaa ccgtgaaaat atcgaacatt ttgtagacta cgctgccttc
     7681 tgttttgaag aatttccaga agtaaactat tggacaacct tcaatgaaat tggaccaatc
     7741 ggtgatggtc aatatttggt tgggaaattc cctccaggta tccagtacga ccttgccaaa
     7801 gtctttcaat cacaccacaa tatgatggtg tctcatgcat gcgcggtaaa attgtacaaa
     7861 gagaaaggct ataaagggga aattggtgtt gttcacgccc tgcctactaa atatcctcta
     7921 gatcctgaaa atccagcaga tgttcgtgca gcagagttgg aagatatcat ccacaataaa
     7981 ttcatcttag acgcaactta tctaggtcgc tattcagctg aaaccatgga aggtgtcaac
     8041 catatcttat cagtcaatgg tggtagtttg gatcttcgtg aagaagattt tacagcatta
     8101 gaagctgcaa aagacttgaa tgatttccta ggaatcaact actatatgag tgactggatg
     8161 gaagcctttg atggagaaac tgaaattatc cataatggta aaggtgaaaa aggaagctct
     8221 aagtatcaaa tcaaaggtat tggtcgtcgt gtagctcctg actatgtatc acgcacggat
     8281 tgggattgga ttatctaccc tcaaggtttg tatgaccaaa tcatgcgtgt gaagaaagat
     8341 tatcctaact acaagaagat ttacatcact gaaaatggtc tcggctataa agatgagttc
     8401 gttgataaca ctgtttacga tgatggtcgt attgattacg tgaaacaaca cttggagatc
     8461 ttgtctgatg cgattgcaga tggagctaat gtaaaaggtt acttcatttg gtcattaatg
     8521 gatgtcttct catggtcaaa cggttatgag aaacgttatg gtctcttcta cgtagatttt
     8581 gaaactcaag aacgttatcc taagaaatca gctcactggt acaagaaagt agcggaaact
     8641 cagattatag actagtagaa ttagtcatta gatatagaat tttagtgagt caaaaagatg
     8701 ttcaaagatt ttatccaatc tatttatgaa aaaaagttta tattataaat ttcgaaaaat
     8761 gctctcaaat accgtgtttg acgagtgaag aattgaaaag tcttggaaaa tggtatgtct
     8821 cgactggtaa agaatggatt tgtcattcag atgatgagct ggaagaattt aaaaatctat
     8881 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8941 cgtttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgta
     9001 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     9061 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     9121 tatagaaaaa agcatagtat caggtgttga aacacctgat actatgcttt ttattgtggg
     9181 aagatttact ttttttcttc tgaaattgag ttgttaccca ggctctttca gtttattaag
     9241 gcttgatgac tttaatgtgt ttagatagct taaaaaggat tgaatcactt agtttagaat
     9301 ctgaaac
//
LOCUS       DAVKFH010000076         9262 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKFH010000076 DAVKFH010000000
VERSION     DAVKFH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.076"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            444..1919
                     /locus_tag="VW006_001865"
     CDS             444..1919
                     /locus_tag="VW006_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9380665.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1885..2600)
                     /locus_tag="VW006_001866"
                     /pseudo
     CDS             complement(1885..2600)
                     /locus_tag="VW006_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2856..3716
                     /gene="speE"
                     /locus_tag="VW006_001867"
     CDS             2856..3716
                     /gene="speE"
                     /locus_tag="VW006_001867"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9380666.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            3713..4972
                     /locus_tag="VW006_001868"
     CDS             3713..4972
                     /locus_tag="VW006_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9380667.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4972..6099
                     /gene="nspC"
                     /locus_tag="VW006_001869"
     CDS             4972..6099
                     /gene="nspC"
                     /locus_tag="VW006_001869"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9380668.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6096..7181
                     /gene="aguA"
                     /locus_tag="VW006_001870"
     CDS             6096..7181
                     /gene="aguA"
                     /locus_tag="VW006_001870"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9380669.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7191..8066
                     /gene="aguB"
                     /locus_tag="VW006_001871"
     CDS             7191..8066
                     /gene="aguB"
                     /locus_tag="VW006_001871"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9380670.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8247..9056)
                     /locus_tag="VW006_001872"
     CDS             complement(8247..9056)
                     /locus_tag="VW006_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9380671.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2716 a         1776 c         2076 g         2694 t
ORIGIN      
        1 tttttatcaa aaaatacttt acaagttctt aaaaacatga tatagtaata aagcttagaa
       61 aatgagatga tgttttctag caaatataaa cccgagtaaa aaatgcctac ggacaggcag
      121 ggttgaatgc cgaagcgtgg ttgaaaagcc acattattga tagggttaaa agcctacttt
      181 tataagttga tgttaggaca cttgtcctaa ttcataaatt tttagtgtgg tgaaagcaca
      241 cgtcatcttg tgaaacgatc aataaagtac gtaatatttg ctactagaga gttaggaaac
      301 atcgggaaca gacatactca acagaaacca aaataaacac gtcagaagat tgcagagcag
      361 gtgaaaacct gctctttttt catgagtcaa cctttagttc cttagttttc ataaggtcct
      421 aaaaatattg aaaggagtat gttttgaaag agttagatca aaaccaagcc ccaatttatg
      481 aggccttggt gaagttacgc aagaaaagga ttgttccctt tgatgttcca ggtcacaagc
      541 gtggacgggg aaatccagaa cttgtcgaac tcttaggaga aaaatgtgta ggcattgatg
      601 tcaattcgat gaaacccttg gataatttag gccatcctat ttcgattatt cgtgatgcag
      661 aggagctggc tgcagatgct tttggagcta gccatgcctt tctaatgatt ggtggaacaa
      721 cttcatcggt gcagactatg attctggcaa cctgcaaggc aggagataag attattctgc
      781 cacgaaatgt ccataaatct gctatcaatg cgttggttct atgtggtgcc attcccatct
      841 atatcgagat gagtgtagat cctaagattg gtatcgcttt aggtcttgaa aatgaccgag
      901 tagcacaggc cataaaggac catccagatg ctaaggctat cctaatcaac aatcctactt
      961 actacggcat ctgttcagac ctaaaggggt tgacagaaat ggctcatgaa gctggcatga
     1021 tggttttagt agatgaagcc catggagcgc atttgcattt cactggtaaa cttccaattt
     1081 ctgctatgga tgcaggagct gatatggcag cagtttccat gcataagtct ggtgggagtt
     1141 tgacacaaag ttcgcttctt ttaatcgggg agcagatgaa ttctgaatac gttcgtcaga
     1201 taattaacct gactcagtct acatctgcct cttacttgtt gatggctagt ttggatattt
     1261 cacgtcgcaa cttggccctt cgtggtaaag agtcgtttga gaaagtcatt gagctatctg
     1321 agtatgcccg ccgtgaaatc aatgctatcg gtggctacta tgcctactca aaagagttaa
     1381 tagacggtgt ttcggtttgc gattttgacg taactaagct gtcagtttac actcagggta
     1441 ttggcttaac aggtatcgag gtttatgacc tcttgcgaga cgaatacgac attcagatcg
     1501 agtttggtga tatcggcaat atcttggcct atatttccat cggcgaccgc atccaagaca
     1561 tcgagcgctt ggttggtgct ctggctgata ttaagagact ctattcaaga gatggaaaag
     1621 atttgatagc aggagaatat attcagcccg agttggtgct gtctccacaa gaagccttct
     1681 attcagagag aaaaagtttg accttagatg agtctgttgg acaggtctgt ggagaatttg
     1741 tcatgtgcta tcctccaggg attcctatct tggctcctgg tgaacgcatt acacgagaaa
     1801 ttgtagacta tatccaattc gccaaggaac gtggttgctc cctccaaggg acggaagatc
     1861 cagaggtcaa tcatatcaac gttattaaga gaaagacaaa ctataagaaa agtcaatagt
     1921 tttatctaaa ctatttctta tttcaatttg atgatttggc gatgatttta gagcacggca
     1981 aaaagccctt gaattagaag cggtcaatcg cttaatttct atcagcttat caaatcctgc
     2041 ctcaatcctt ttctgaggat tagggtagcg tgtcaagagt tggtaggtat attctgaatg
     2101 ctttccaacg attttatcca actcaggaaa gatgatatca agacaacgag tgtattgtac
     2161 tttccaatca gactgttttt cttgagacga tgaatatgtc tagccagtat ttttagttct
     2221 acttgccgat tatcgtgttg aaattgttca cgattggggt cagaaagaag tttaagagcg
     2281 atgccatgag cgtctttctt atccgtttta gtcttgtgaa gtgataatga tttggcaaat
     2341 tccttgatga gcaaaggatt gtaggtgtaa actttatatc cttgttcatg caggaagttc
     2401 agtagattaa aggcataatg tccggtattt tcaagagcga tgagacagtc ttggttgagt
     2461 tgtcgaagag acagatctaa gagttcaaaa ccagctttat tatttgaaaa agtgagtggt
     2521 ttaagaacag tttttcctgg aacattcaag gctgtaacat cgtgtttatt tttagcgaca
     2581 tcaatgccca cataaagcat gggagtacct ccagatgtag tatttcaagt ctacttggtt
     2641 atccacgaat tttttgcctt gttaccttag acgagataaa acgtctatgc gttatcaaac
     2701 tcattaccaa ttgaaacaaa aaactgtggt tagagccttt cggaaatcgt caagcgattg
     2761 gagggaataa actaatccac agtggcttat tccaagtata ccacttgggc tttggcagta
     2821 gctaactgcg ctaaatataa tataaggaga aatagatgga tttatggttt tctgaagttc
     2881 atactccaga tgtcaaattg tctctgagaa cagccaagca actttacgct ggaaaaagtg
     2941 aatggcagga tatcgaagtc ttggatacgc cagcttttgg gaaaatactg attttaaatg
     3001 gccatgtctt gttctcagat gcggatgatt tcgtctacaa tgaaatgacc gttcacgttc
     3061 ccatggctgt ccacccaaat ccaaagaaag tattggttat tgggggtggt gacggcggtg
     3121 ttgcccaagt attaacactc tatcctgaac tggaacaaat cgatatcgtg gaaccggatg
     3181 agatgttggt cgaggtctgt cgtgagtatt tcccagactt tgctgcaggg ctagatgatc
     3241 ctcgtgttac catttactac caaaatgggc tacgcttttt gcgaaactgc gaagatgatt
     3301 acgatattat catcaacgat gcgacagatc catttggcca tacggaagga ctctttacca
     3361 aggaattcta cggcaatagt tatcgagctc tgaaggaaga cggcatcatg atttaccagc
     3421 atgggagtcc cttctttgac gaggatgagt cggcctgccg aagcatgcac cgcaaggtca
     3481 atcaagcctt tccaatcagt cgggtttatc aggcccatat tccaactagc ccagctggct
     3541 attggttgtt tggatttgca tcgaaaaaat accaccctgt caaagatttt gacaaggaag
     3601 gttggaaaaa acgccagctt ttcacagaat actacactgc aaacttacac gtgggatcct
     3661 ttatgttgcc caagtatgtt gaggacattt tagaagaaga ggaaggaaaa aaatgagtcg
     3721 tttactagtt attggttgtg ggggcgttgc ccaagttgct atttcaaaga tttgtcaaga
     3781 tagcgaaaca tttacagaga ttatgattgc tagccgtacc aagtcaaaat gcgatgactt
     3841 gaaagcgaag ctagaaggca aaacaagtac taaaattgaa actgcagcac ttgatgctga
     3901 caaggttgaa gaagtgattg ccctgattga aagctacaaa ccagaagctg ttttgaatgt
     3961 agctctgcct tatcaagatt taaccattat ggatgcttgt ttggcaacag gtgttcacta
     4021 tatcgataca gccaactacg aagcagaaga cacagaagac cctgagtggc gtgctatcta
     4081 cgaaaaacgt tgtaaggaac ttggttttac agcctacttt gactactcat ggcagtgggc
     4141 ttatcaagag aaattcaaag aagcaggctt gactgctctt cttggttctg gttttgaccc
     4201 aggtgtaact agtgtctttt cagcttatgc cctcaaacac tattttgatg aaatccatta
     4261 tatcgacatt ttagactgta atggcggtga ccacggttat ccatttgcaa ccaactttaa
     4321 tccagaaatt aatctccgtg aggtttctgc gccaggttct tactgggaag atgggaaatg
     4381 ggtcgaagtc gaagctatgt ctatcaagcg tgagtatgat ttccctcaag ttggacaaaa
     4441 agatatgtat ctccttcacc atgaagaaat cgaatcattg gccaagaaca ttccaggtgt
     4501 caaacgcatt cgtttcttta tgacttttgg tcaatcttac ttgacgcaca tgaaatgtct
     4561 tgaaaatgtt ggactccttc gtacggatac cattaacttt aacggccaag aaattgttcc
     4621 aattcaattt ttgaaagcct tgcttccaga tcctgctagc cttggcccac gtactgtagg
     4681 taaaaccaat attggatgta tctttacagg tgtcaaagat ggcgttgaaa agactatcta
     4741 catctacaat gtttgcgacc atcaagagtg ctatgcagaa gtcgggtcac aagcaatttc
     4801 atacacgaca ggcgttccag ccatgattgg gacaaaatta gtcatgaacg gaacttggaa
     4861 acaagctgga gtgtataacc ttgaggagtt agatccagat ccattcatgg aagctttgaa
     4921 tgagtatggt ttgccatggg ttgtggttga aaatccacaa atggtggact aatgaagtta
     4981 gaacaagtac caacaccagc ctatgttatt gacttggcca agttagaagc taattgccgc
     5041 attctacaat atgtacaaga agaggccggt tgcaaggtct tgcttgccca gaaggcatat
     5101 tccctctaca aaacttatcc cttgattagc cagtatctat caggtacgac agctagtgga
     5161 ctctatgagg ccaaattggc aagggaagaa tttcctggtg aagtccatgt ttttgcacca
     5221 gctttcaagg atgaggacat ggaggaattg cttgggataa cagaccatat cgtctttaac
     5281 tcagagagac agttgcgtaa gcatggggct cgttgtcgag aggctggtgt cagtgttggt
     5341 ttgcgcctca accctcagtg ttcaactcaa ggagatcacg cgctctatga cccttgtgca
     5401 cctggttctc gctttggagt tactatagac aagattccga gtgatttgct agatttggtt
     5461 gacggacttc attttcatac cctttgcgag cagggagcag atgatttaca aacaactttg
     5521 aaagcagtag aagaacagtt tggtccctac ttacatgagg taaaatggct caatatgggt
     5581 ggtggtcatc atattacaag agaaggttac gatgtggatt tgctgatttc agaaatcaag
     5641 cgtatccgaa aaacttacaa tcttgaaatc tatatcgagc ctggtgaagc cattgcgctt
     5701 aatgcgggtt atttagcaac tgaggtatta gatattgtag aaaacggtat ggaaatcttg
     5761 gttttagacg cctctgcgac ctgccatatg cctgatgtac ttgagatgcc ctatcgtcca
     5821 cctttgagaa atggctttga gtcacaggaa aaagcccata cctacagact ttcttctaat
     5881 acctgtctga ctggcgatgt gattggtgat tatagttttg aaaatccagt ccaaatcgga
     5941 gacagacttt attttcaaga catggccatt tattcttttg tcaaaaataa tacctttaat
     6001 ggtattggat tgccaagtct ctatctcatg gacgaacagg gagactgtag cttactcaaa
     6061 gcttttggct atcaagactt taaagggaga ttatcatgat ggacagtcca aaaaaattag
     6121 gctatcacat gccagcagag tacgaacccc atcatggtac cctcatgata tggccgactc
     6181 gaccaggatc atggcctttt caaggaaagg ctgctaaaag agcatttact cagatcatcg
     6241 agaccatagc agaaggggaa agagtctatc ttttggtgga gcaggcctat ctatctgaag
     6301 cccaatccta tcttggagac aaggttgttt atttagacat tcccaccaat gatgcctggg
     6361 cgcgtgatac tggcccaacc attctcgtca atgataaagg taagaaatta gccgtggatt
     6421 gggcctttaa tgcttgggga ggcacctatg atggtcttta tcaagattat gaagaggatg
     6481 accaagtagc cagtcgtttt gctgaggcct tggaaaggcc tgtctatgat gctaaacctt
     6541 ttgtactgga aggaggcgca atccatagcg atggtcaagg aactattctc gtaactgaaa
     6601 gttgcttgct tagtcctggt cgcaatccta acctgactaa agaggagatt gaaaacacat
     6661 tattagaaag tcttggtgct gaaaaagtta tttggcttcc ttatggtatt tatcaggatg
     6721 aaaccaatga acacgtcgat aatgttgttg cctttgttgg tcctgctgag gttgttttgg
     6781 cttggacaga tgacgaaaat gatccccagt atgccatgtc aaaagcagat ctcgaactct
     6841 tagaacagga aacagatgca aaaggttgtc acttcaccat tcataaattg cctatccctg
     6901 cagttcgaca agttgtgaca gaagaagatt tgccaggcta catctatgaa gaaggagaag
     6961 aagagcgata cgcaggtgaa cgactagcag cttcctacgt aaacttttat atcgccaaca
     7021 aggctgtctt ggttccacag tttgaggatg taaacgacca agtggcctta gatatcctca
     7081 gcaagtgttt cccagaccgt aaagttgtcg gaataccagc cagagatatt ctcttaggtg
     7141 gtggcaatat ccactgtatc acccaacaaa ttccagaata ggagaaaaag atgagaaatg
     7201 taagagttgc aaccattcag atgcaatgcg ctaaggatgt ggcaacaaat atccaaaccg
     7261 cagagcgttt agtacgtcag gctgctgagc aaggagccca aattattctc ttgcccgagt
     7321 tgtttgaaca tccctatttc tgtcaggaac gtcagtatga ctactaccag tatgcccaat
     7381 ctgtagcgga aaatactgcc attcagcatt ttaaggtgat tgctaaggaa ctacaagttg
     7441 ttttaccaat cagtttctat gaaaaagatg gtaatgtctt gtataactct attgccgtca
     7501 ttgatgcaga tgggggagtg ctgggcgttt atcgaaagac ccatatacca gatgaccatt
     7561 attatcaaga aaaattctat ttcacgcctg gtaacactgg tttcaaggtc tggaatactc
     7621 gctatgctaa gattggtatc ggtatctgtt gggatcaatg gttccctgaa acagcccgct
     7681 gtcttgcatt gaatggtgct gaattgctct tttatcctac agctatcggt tcagagccaa
     7741 ttttggatac agatagttgt ggtcactggc aacgtactat gcaagggcac gcagcagcga
     7801 atattgttcc agtcatcgca gccaatcgtt atggtttaga ggaggttact cctagtgagg
     7861 aaaatggcgg acagagttcc agtcttgact tctacggttc ctcctttatg acggatgaaa
     7921 caggagctat tctagaacga gctgaaagac aagaagaagc tgttctgtta gctacttatg
     7981 acctagacaa gggagcaagt gaacgtctaa actggggctt gtttcgagat agaagaccag
     8041 aaatgtatag acaaattaca gattagtgtg ggagaaatga gagattcatt ctgctagact
     8101 aacttcttat tagcaactat aagatactat ggcatctagt aaatcgattt ttatgattcg
     8161 ctattcttgt ctattgatta gtccgtattt taaaatatta gcaaaaaagc aaatagcagt
     8221 aacttctgtc tatttgcttt tcttttttat agaatatatt tctcaatagc acgcgcaacg
     8281 ccgtcttctt cgttgcttga ggtaacggca tccgcaagag atttgacata atcgctggca
     8341 tttcccattg caatcccaag ccctgcaaac tggagcattt cgatatcgtt attagcatcg
     8401 cccatggcca taatctctga ggaatcaatc ttcaaaatct cagctagtcg tgaaagagca
     8461 gtagcctttg tcgttccaag cggcattgct tcataaatga caggctgcga acgaactcca
     8521 ctgaatcgtt ggcaaagctc ttcagcaaaa cgctgctcaa aatcgtctgt ttgttctttt
     8581 gttcctaaaa acataccttg gaacatccgg aactttccac tagtcgcttc ttcaagagaa
     8641 atttcagtca ggtctgaaaa tactagttta gcatcatttt caataacttg attgggtttg
     8701 ccaccgagaa caaaataatg tgactcgtca aaaagtgtca actgaacatc actcttttca
     8761 gcaaggtcat agaggtattc gatgtcagct ggactcagtt ctttccagtc aactagactc
     8821 caatcactgg tctggtgagt tgaacaaccg ttgttaacaa taatatattc gttctggagg
     8881 tcaagctcca gttttttgta gtaggggagg acaccgaaaa gggggcgacc cgtacagaga
     8941 accagtttga cacctttttc aatggctttg tgaatagcag taatgtgagc ttgtgggatt
     9001 tccttggctt cattgaggag ggtgccgtcc atatccaagg ctagtagttt aatcataggt
     9061 cttccttctt tatcttttgc tattattata gcatattttg gagaagaaat tgatagaaag
     9121 cttgagacta attgatttta tagtttaaga tgttttgatg acaattcatg atttgaagag
     9181 gatatttggc aaagatatgc tatactatgt ttgtcaatgt tgcaactaga caaattaaaa
     9241 aaccaactta atataatagt tt
//
LOCUS       DAVKFH010000077         8729 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKFH010000077 DAVKFH010000000
VERSION     DAVKFH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.077"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            13..1014
                     /locus_tag="VW006_001873"
     CDS             13..1014
                     /locus_tag="VW006_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9380672.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1226..1455
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1621..1698
                     /locus_tag="VW006_001874"
                     /pseudo
     CDS             1621..1698
                     /locus_tag="VW006_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2245..3606
                     /gene="trpE"
                     /locus_tag="VW006_001875"
     CDS             2245..3606
                     /gene="trpE"
                     /locus_tag="VW006_001875"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9380673.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNSFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGVTDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3603..4169
                     /locus_tag="VW006_001876"
     CDS             3603..4169
                     /locus_tag="VW006_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9380674.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4180..5184
                     /gene="trpD"
                     /locus_tag="VW006_001877"
     CDS             4180..5184
                     /gene="trpD"
                     /locus_tag="VW006_001877"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9380675.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDVRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5181..5948
                     /gene="trpC"
                     /locus_tag="VW006_001878"
     CDS             5181..5948
                     /gene="trpC"
                     /locus_tag="VW006_001878"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9380676.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLNYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5935..6534
                     /locus_tag="VW006_001879"
     CDS             5935..6534
                     /locus_tag="VW006_001879"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9380677.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6512..7735
                     /gene="trpB"
                     /locus_tag="VW006_001880"
     CDS             6512..7735
                     /gene="trpB"
                     /locus_tag="VW006_001880"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9380678.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7728..8504
                     /locus_tag="VW006_001881"
     CDS             7728..8504
                     /locus_tag="VW006_001881"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9380679.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8476..8577)
                     /locus_tag="VW006_001882"
                     /pseudo
     CDS             complement(8476..8577)
                     /locus_tag="VW006_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         2599 a         1621 c         2085 g         2424 t
ORIGIN      
        1 tggaggactg aaatgaaaaa tataaatggg aaaaaggtaa ctatttatga tattgcacgt
       61 ttatcaggtt tttctccaaa aacagtttca cgagttatta atggtggagt aaatgttaaa
      121 gaagaaactt atcaagcgat tcaaaaagtt atagaggaat tgtcgtatat tccaaatgcg
      181 tatgcaaaaa atttaactaa aaaagaagct ataaatatat tgatttctgt aaaaaaaatc
      241 gactcttttc ctttaatttg gtttcatacg ctgttagata aagtgttacg gacgtgtaaa
      301 gaatttggtg ttaatgctat agttgagtac tttggagagg aggatacaat tagtaattca
      361 attatatcaa gcacaggtag cctagtagat ggtgttattg ttttttatga aagtgtagat
      421 gatatcagaa ttcagtattt aaagaagaac catatgcctt ttcttgtttt cggtgaatct
      481 caaacgtctg gagtagtcta tgtatctaat aataattttc aagctactta tgatatgatg
      541 aaagctgtaa ctgaagaaaa atttaaaaat atgtggttgc ttatgggagg agaatcccat
      601 gttaataagg atcgtgaaag aggtgtgcgt tcttttctga atgataaaaa ttactttatg
      661 gatttaaaag tcatttatgg cttatctaca attgattcag tttattctta tgcaatgcag
      721 cacttgacta cccaaaatta tccggacatt atttttgttt caggagatga aaaggttcag
      781 ggcttgattc gtgcgtgcta tgagaaagga attcttattc cagatgatat ttctatcatt
      841 ggatttgata atattcctat ttctcagtat tatactccag ctttgtctac aattgctccc
      901 aattatgtta aattggctaa agaaatgata gaaggtgttt tagcaattat taaaggggaa
      961 agtgtcacat ctgttgaagt ttctcctaaa tttgttagga gacagagttt ctagtattgt
     1021 ttaattcttg aaaatcctag tggaaaagat ggagtgttct aaaataagtt ctgtttagcc
     1081 tccattcagt ttctattgag ttggatccaa tttctaacaa gtgggacatg ttcatgttcg
     1141 atctaaggaa gacatggtca cattcgaatg atattttcaa aaaaatcgta aaaaagtctt
     1201 gacaaagaaa aaacaaagta ttaaaataag ttcaacaaat cagaaaagta actatttttg
     1261 atcttcaggg agcctgtggt ggttgtgaat aggtggttgg aagtagtgaa agtgggctga
     1321 ttttgaaaat gaatttgaaa caatgaaaat tcggtgcgca caccttacag tgcagcttgt
     1381 tgttaaacaa ggcagagatg taaagggata gtccctttat aattgaggtg gcaccgcgtt
     1441 accaacgccc tcacacggaa gtatattttg tgtgtgggct tttttctatc cgtcgtttgg
     1501 tttatctttt attagggcct taagtcgctt tgatgaactt gagttctatc tacagctcct
     1561 tgcctactac taaaagcaaa caaaaagctg gactaatacg aaaaaagcta gtaatactca
     1621 atgaaaatca aagagcaaac taggaaacta gccgcaggtt gctcaaaaca ctgttttgag
     1681 gttgtggata gaactgacga agtcagctca aaatactgtt ttgaggttgt ggatagaact
     1741 gacgaagtca gctcaaaaca ctgttttgag gttgcagata gaactgacga agtcagtaac
     1801 catacctacg gcaaggcgac gttgacgcgg tttgaagaga ttttcgaaga gtataaaggt
     1861 gtatcgaaat aaaaatctcc tcatcaatat ctctggtcgt ggaggcaagg acctagctgc
     1921 gattgcagat tacctagaag ctaaaaaata atagatggaa aaattacagt cttttctctc
     1981 acagagagct tgtggttgct gaaaacaagc agagaaagct gtaaagttgg gttcatctga
     2041 aacgagagaa gaaaacatca ttctcaaggg agtgcccttt atcgcacagc ctgttacagg
     2101 aggtatctga gacaggaatg agagatagga gaaatcctat aattgaggtg gcaccgcgaa
     2161 tttcgtcctc acgcaagtta ttttgcgtgg ggatttttca tacagtcacc acggactaga
     2221 agtagaactg aaagggaaat tacaatggaa cgaatcattc atggagatgt cttatcacca
     2281 atcttggctt atatgcgctt aaaggggcaa cacaaggtta tcttagagag tattccgaga
     2341 gacaaggaaa cagctcgttt ttctatctta gcctataatc ctgtttttga gattaagttt
     2401 gaaaatggag ttctttatca aaatggtcaa gtgattgatc gagatccctt ggatttcctt
     2461 tatgaagtga ttcataagag tcagcaccac tcagagctac cttttggtgg tggagccatt
     2521 ggttttgtgg gttacgatat gatttcgctt tatgaagaaa ttggtcaaat ccctgaggat
     2581 acaattggga cgccagacat gcatttcttt gtctatgaga gctatatggt ctttgaccac
     2641 aagaaggaga agatacatgt catagaggat gctctctata gtgagcgcag tcaagaggcc
     2701 ttggaaaaat ccttgaacca agtgcttgag gaattacgca ttcctgctcc aaatgaattt
     2761 gaagacttgg atttatcacc acttgacttc aaaccgcata tcgctccaca taagtttgag
     2821 gtaatggtag aaacagctcg tgacttgatt cgtaatggtg atatgttcca atgtgtactc
     2881 agccagcgtt tctcagcaga agttactgga aattcatttg acttctacag aaatctccgc
     2941 gtgaccaatc catccaatta cctctatttc tatgattttg gggattatca aatcatcgga
     3001 gctagtccag aaagtttagt ttctgtcaaa aatggcatcg tgacaaccaa tccgattgca
     3061 gggacgcgac caagaggggt tacggatgaa gaagacaaga ccttggcgac tgacctcctc
     3121 tctgatgaga aggaaacagc agaacatcga atgttggtag acttggggcg taacgatatt
     3181 ggccgcatct ctgaaacggc cagtgtccaa gttactaagt atatgaaggt ggagctcttc
     3241 cgctacgtca tgcatttgac cagcgtggtc aaggggcgtt tgcttgcaga actcactgcc
     3301 atggatgcct tgaaagcaac gcttcctgct ggaaccgttt ctggagcacc aaagattcgg
     3361 gcgatgagac gcatctatga actggaaacg gaaaaacggg gcgtatatgc aggagcaatc
     3421 ggctacttgt ctgcgacggg tgatatggat ttggccattg ccatccgaac catgattctc
     3481 aaaaatcaaa gagcctatgt gcaggctggg gcagggattg tctacgactc tatcgcccaa
     3541 aacgaatacc aagaaaccat taacaaagca aaatctatga ctagaattgg agaactaaga
     3601 ccatgatttt attgattgac aactatgatt cttttaccta taacttggcc caatacattg
     3661 ggaattttgc agaagttcag gttctgagaa atgatgattc caagctgtat gaagaagctg
     3721 aaaaagcaga tggtctggtc ttttctcctg gtcctggttg gccagttgat gctggaaaga
     3781 tggaagacat gattcgtgat tttgcaggca agaagccgat tctagggatt tgtttgggcc
     3841 accaagccat tgcagaagtc tttggtggta agctaggttt ggctccaaaa gtcatgcatg
     3901 ggaaacagag caatatcaac tttgaagcgc catctgtttt gtatcaaggt attgaggatg
     3961 gacgtgcggt catgcgttat cacagtattt tgattgagga aatgccagaa gactttgaag
     4021 tgacagctcg ttcgactgat gaccaagcca tcatggggat tcaacataaa aacctaccga
     4081 tttatggctt ccagtaccat ccagagagca ttggaacgcc agacggcttg tcttctattc
     4141 ggaattttat cgagaaggtt gtaaagtgag gaaactagga tgaaagagat tattgaaaaa
     4201 ctagcaaaat ttgaaaattt atcaggtgtg gaaatgacgg atgtcattga gcgtatcgta
     4261 actgggcgtg taactgaagc gcagattgct tctctcctct tagctcttaa gatgaagggg
     4321 gaaacacctg aagaacgcac agccattgcc caagtcatga gaggacatgc ccagcatatt
     4381 ccaactgaga ttcatgatgc catggacaac tgtggtacag gtggggacaa gtctttcagt
     4441 tttaatattt ccacaactgc agcctttgtc ttggctggtg gcggtattca catggccaag
     4501 catggtaacc gctcgatttc ttctaaatct ggttccgcag atgtcctcga agccttggga
     4561 atcaatcttg acctcaaacc agctgaacta ggtaaggtct ttgataaaac tggaatcgtc
     4621 tttctcttcg ctaaaaatat gcacccagct atgaaataca tcatgccagc tcgtttggaa
     4681 ttgggaattc caacgattat gaacttgact ggtccactga ttcatccaat ggctttagaa
     4741 acacagcttc ttggaattag tcgtccagaa ctcctagaaa gtacagctca ggttttgaaa
     4801 aatatgggtc gcaaacgtgc catcgtggtt gctggaccag aagggttgga tgaagctggc
     4861 ttgaacggaa caaccaagat tgcacttctt gaaaatggcg aaatcagctt gtcaagcttt
     4921 actccagagg atttgggaat ggaaggctat gctatggaag atgttcgtgg tgggaatgct
     4981 caggaaaatg cagaaatttt gcttagcgtt ctgaaaaacg aagcaagtcc attcttggaa
     5041 acgacagtct tgaatgctgg tcttggtttc tatgctaatg gtaagattga tagcatcaag
     5101 gaaggagttg ccttggcccg tcaagtgatt gctagaggca aggcccttga aaaactcaga
     5161 ctgttacagg agtatcaaaa atgagtcagg aatttttagc acgaatctta gagcagaagg
     5221 cgcgtgaggt ggagcaaatg aagctggagc aaatccagcc tctgcgccag acctatcgct
     5281 tggcagaatt tttgaagaat catcaggacc gcttgcaggt aatcgctgag gtcaagaaag
     5341 ctagccctag tttgggagat atcaatctcg atgtggatat tgtgcaacag gcccagactt
     5401 atgaagaaaa cggagcagtg atgatttcgg tgttgacaga tgaggttttc tttaaagggc
     5461 atttgaatta tctacgggaa atttccagtc aggtagagat tccgacgctc aacaaagact
     5521 ttatcataga tgaaaagcaa atcatccgcg ctcgcaatgc aggtgcgaca gttatcttgc
     5581 ttattgtggc agccttgtcc gaagaacgcc tcaaggaact gtatgactac gcgacagagc
     5641 ttggtctgga agtcttagtg gagactcaca atctagctga actagaggta gcccacagac
     5701 ttggtgctga gattatcggg gtcaacaacc gcaacttgac tacctttgag gtcgacttgc
     5761 agaccagtgt agatttagcc ccttactttg aggaaggtcg ctattacatt tctgaatctg
     5821 ccattttcac agggcaggat gcggaacgac tagccccata ctttaacgga attttggtag
     5881 ggacagctct tatgcaggca gaaaatgtgg cccagagaat caaggagttg cagattgaca
     5941 aaggttaaaa tttgtggact atcgaccaaa gaagcggtgg aaacagccgt ttcagcagga
     6001 gccgactata tcggttttgt ctttgcacct agtaaaagac aggtgacttt agaagaggca
     6061 gctgagttgg caaagcttat tcctgcagat gtgaaaaagg ttggagtatt tgtttcacca
     6121 agtcgggtag aactgctgga agcgattgac aaagttggct tggacttggt tcaagttcac
     6181 ggtcaggtgg cagctgattt atttgagaat ttgccttgtg ccagtattca ggctgtgcag
     6241 gtagatggaa atgggcatgt ccccaattct caggcagatt atctactctt tgatgcccct
     6301 gtggcaggaa gtggtcagtc ctttgattgg ggtcaactgg atacgactgg actagcacag
     6361 cccttcttta tcgcaggtgg ccttaatgaa gataatgtag taaaagcaat tcaacatttt
     6421 actccctatg cagtagatgt atcgagcgga gtggagacag atggacaaaa agatcatgaa
     6481 aagattagaa gatttataga gagggtaaag catggcatat caggaaccaa ataaagatgg
     6541 attttacgga aaattcggcg gacgttttgt cccagaaaca ttgatgacag cagttttgga
     6601 gttggagaag gcctaccgtg aaagtcaggc agacccaagt ttccaagagg aattaaacca
     6661 gcttttgcgc cagtacgtag gacgtgaaac tcctctttat tacgcaaaaa acttgaccca
     6721 gcatatcggc ggagccaaga tttatctcaa acgggaagac cttaaccata caggagccca
     6781 caagattaac aatgccttgg gacaagtttg gcttgccaaa cgcatgggta aaaagaaaat
     6841 tatcgcagaa actggtgctg gtcagcacgg tgtggcaact gcaactgctg cggccctctt
     6901 taacatggaa tgtaccatct acatgggtga ggaagatgtc aaacgccaag ccctcaatgt
     6961 cttccgtatg gagcttttgg gagctaaggt cgaggccgtg acagatggtt cgcgcgtgct
     7021 caaggatgcg gtcaatgcag cccttcgttc atgggtggct aatatagatg atacccacta
     7081 tatccttggt tctgccttgg ggcctcatcc atttccagaa attgttcgtg acttccaaag
     7141 tgtcattggt cgagaggcta aacaacagta ccgtgacttg acaggacaaa atctgccaga
     7201 tgccctagta gcctgtgttg gtggtgggtc gaatgccatc ggtctcttcc atccctttgt
     7261 agaagatgag tcagtagcca tgtatggagc tgaagcggct ggatttggtg tggatacgga
     7321 gcaccacgca gctaccttga ccaagggtcg tccaggtgtc cttcacggtt ccctcatgga
     7381 tgtgctccaa gatgcccatg gtcaaattct tgaagccttc tctatctcag caggtttgga
     7441 ctatcctggt atcggtccag agcattctca ctaccacgat attaaacgtg ccagctatgt
     7501 ccctgtgaca gacgaagaag ccttggaagg attccaactc ttgtctcgtg tggaagggat
     7561 tatcccagct ttggaatcta gccatgctat cgcctttgcg gtgaaattgg ccaaagaact
     7621 tggaccagaa aagtctatga ttgtatgcct atcaggtcgt ggggacaagg atgtggttca
     7681 agtaaaagac cgcttggaag cagatgcagc aaagaaggga gaagctcatg cctaagacac
     7741 taacaaaaaa attaaatgct attaaagcag ctggaaaagg aatttttgtt ccctatatca
     7801 tggctggtga ccacgaaaaa ggtttggatg gtcttgctga aacaattcac tttttagaag
     7861 atttgggtgt ctctgcaatt gaagtgggta ttcccttttc agaccctgtt gcagatggac
     7921 ctgttatcga agaagctggc ttgcgcagtt tagcccacgg gacctctacc caggctttag
     7981 ttgaaacctt gaaaaccatt gaaacagaga ttccactggt catcatgacc tacttcaacc
     8041 ccctctttca gtacggtgtg gagaactttg tcaaagatct ggctgataca gctgtgaagg
     8101 gcttgattat cccagacctg cctcatgaac atgccaactt tgtagaacca tttttggcag
     8161 atacagatat cgccttgatt cctttagtaa gcttgaccac aggaattgag cgccagaaag
     8221 agttgattga aggggcggag ggattcgtct atgccgttgc catcaatggg gtaacaggga
     8281 aatcaggcaa ttaccgtgca gatttggaca agcacttggc gcaacttcat caagtggccg
     8341 acatcccagt cttgacaggt tttggtgtat ctagtcaagc cgatctggaa cgcttcaatg
     8401 cggtgtcaga tggtgttatc gtcggttcga aaatagtaaa agctctccac caaggagagc
     8461 cgattcagga ctttatcaaa caagcagtag cttaccaaaa ataattaaac aagtagcaag
     8521 taagaggaaa ggcacgaaag gaagtctttc ctttttcttt tgcaggagaa aggctagttt
     8581 tatagtgaat tgaatctgga atattacact acggttccta aaatatttta agaaagtgat
     8641 ttgaatttcc caataaattt gttcagtttt taattgattt tactatagaa gatataattt
     8701 tctaaattac tctgacacag aactttaag
//
LOCUS       DAVKFH010000078         8480 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKFH010000078 DAVKFH010000000
VERSION     DAVKFH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.078"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..259
                     /locus_tag="VW006_001883"
     CDS             <1..259
                     /locus_tag="VW006_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU9380680.1"
                     /translation="MNRAISCYREEQSGCLDMVVIQKQNEIEELSTNIEKKIMNYIFE
                     DDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            272..1747
                     /gene="galT"
                     /locus_tag="VW006_001884"
     CDS             272..1747
                     /gene="galT"
                     /locus_tag="VW006_001884"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9380681.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1760..2740
                     /gene="galE"
                     /locus_tag="VW006_001885"
     CDS             1760..2740
                     /gene="galE"
                     /locus_tag="VW006_001885"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9380682.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGLPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
     gene            2950..3609
                     /locus_tag="VW006_001886"
     CDS             2950..3609
                     /locus_tag="VW006_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9380683.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3634..4701
                     /locus_tag="VW006_001887"
     CDS             3634..4701
                     /locus_tag="VW006_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9380684.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELASKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWGVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4804..5814
                     /locus_tag="VW006_001888"
     CDS             4804..5814
                     /locus_tag="VW006_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380685.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5826..7517
                     /locus_tag="VW006_001889"
     CDS             5826..7517
                     /locus_tag="VW006_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9380686.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7520..8230
                     /locus_tag="VW006_001890"
     CDS             7520..8230
                     /locus_tag="VW006_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9380687.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2873 a         1168 c         1663 g         2776 t
ORIGIN      
        1 tatgaatagg gctatatctt gttatagaga ggaacaatct ggatgtttag atatggttgt
       61 tatacaaaaa cagaatgaga tagaagagct tagtacaaat attgaaaaga aaataatgaa
      121 ttacatattt gaagatgatg ggaatgtttc tgaagtaatt ggtgctttag atattattca
      181 tcaccttgat aaaattgctc atactacgca atcaatttat aaatggataa tgtatagaaa
      241 atatgggaac ataaattaga aagaaaaata tatgaaaaca attattgata attttgttga
      301 tagagtaatt gaatacggaa tgtataatga aattgacaaa atatatgtga aaaatagagt
      361 actagcgtta ataggtgagg agggaactga tcgcatttcc gatgaaaatg acctgaaaca
      421 aataaaagat tacttagtgg aaatcgcctt aaaaaacggt aaaattaaag atttaatcga
      481 ggaaaaggaa tgtttaggag cagagttaat gaatttcata gttcctttac ctagtcgatt
      541 aaatgatatt ttttggagtt catatgatat atcacctcaa gaagcagttg aagagtttta
      601 taagttaagt aaagatagtg attatattaa aacttctgct attgctaaga atattgagtt
      661 tcgagcatca actaaatacg gagagctaga gattacgata aatttgtcaa aacccgaaaa
      721 agatccaaaa acaattgctg ctgaaaaatt agtaaaagcc actaattatc ccaaatgttt
      781 actatgtatg gaaaatgaag gatatcaggg gcgaataaat tatccagcac gttcgaatca
      841 tagaattatt cgattgaaac ttggtgacga agtatgggga ttccaatatt ctccctactt
      901 atactttaat gagcatgcga tatttttaaa tagtcaacat gtaccaatgg ctattacctc
      961 taaaacattt gaacaattat tggagattgt tgatattttg ccaggatatt ttgctggttc
     1021 aaattctgat cttcctatct caggaggctc cattcttagt cataaccact accagggagg
     1081 gaaacatatt ttcccaatgg aaaaagcaaa atttgagagt gaatttcgtt ttaaagactt
     1141 tgaggatgtt aatgctggta ttgtaaaatg gccaatgtca gtaattaggt tacaaagtga
     1201 aaataaaaat cgcttgttag atttagctac taaaattcta aataagtgga gagaatattc
     1261 agatttagaa gtagacatca ttgctatgac tgaagatgtc ccacaccata cggtgactcc
     1321 tattgctcgt aaagtggacg ggagatatga attagatatt gttctaagag ataatcatac
     1381 tactgaacag tacccagatg gtgttttcca tcctcatcaa gatgtacaac atattaaaaa
     1441 ggaaaatatc gggttaatcg aagtgatggg acttgcgata ttaccacctc gtttgaaacc
     1501 agagttagaa gaagttggaa aatatctttt aggagaggat aacgcaattg cggattatca
     1561 tttagaatgg gccgaccaat tgaaagaaaa atatcctcgc attaataaag aagaggttaa
     1621 tagcgtagtt caacacgaag caggacaagt gtttgcgaga gttctggaag atgcaggagt
     1681 ttataagaat acaccgtctg ggcatgaagc gtttatgaga tttgttaagt cggttggaat
     1741 taattaggag gaaacaaaaa tggcaatatt ggtaacaggc ggagctggtt atattggtag
     1801 ccataccgta gtagaattac taaatttagg aaaggaagtc atcattgtcg acaacctttc
     1861 gaactctagc atcttggtgc tagaccgtat tgaagcaatt acaggaatac gtcccgtgtt
     1921 ttacgaatta gatgtttgtg ataaaccagc attgagaaag gtatttgaac aagaatcgat
     1981 tgatgctgca attcattttg caggttataa agctgtcgga gaatccgtgc aaaagcctgt
     2041 gatgtactac aaaaataata ttatgagtac attggcactt gttgaagtga tgtcagaatt
     2101 taatgttaaa aagattgtct tttcttcaag tgcgactgta tatggaatta acaatcagtc
     2161 acctctaatt gagacgatgc aaacaagtgc gacaaatcct tatgggtata cgaaagtgat
     2221 gcttgagcaa attttaaaag atgttcatgt ggcagattca gaatggagta ttgcgttgct
     2281 tcgttatttc aatccaattg gtgctcatga gtctggtttg attggggaag atccctcagg
     2341 acttcctaac aacttgatgc cttatattgc acaagtagcg gtaggtaagc tatcagagct
     2401 aagtgtgttc ggaaatgatt atgatacgct tgatggtact ggtgtgcgtg attatatcca
     2461 tgtagtagat ttagcaatag gacatataaa agctttagaa aaagtatctg aaaaaacaga
     2521 tgtttatatc tataacctag gctcgggaga aggcactagt gtattacaac ttgtaaatac
     2581 atttgaaagt gttaataaga tccctattcc ttataaaata gttccaagac gttcaggaga
     2641 cgttgcaact tgttatgcaa atgcagacaa agcatataag gaattaaatt ggaggacaac
     2701 aaaatcgatt gaagacatgt gtagagatac atggaattag caatcaaaaa atcccaatgg
     2761 ctataattga tgatactgtc tatatgagtg ctgtattttg gaaaaattac ttaacctttt
     2821 aatgaaatgc atatttaatg aaagataaat taaaaaaaat gtttgacagc gcttgacaaa
     2881 ggaaacggtt tcattgtata atgtaaacat agtgacaccg atgtcaaata taaaaaagaa
     2941 ggtctttata tgaaaaactt aacaaaaatt aagttcaaag aaaatgggga atttaatcat
     3001 ttccctggga atacagttgt agcaaatctt tatactaaac aagatttgat ggaagttgtt
     3061 gatattattc aatcacgtta tagagaatta ccatttatcg ataagtttac tttaactcca
     3121 aggaattcta ttcacatgac tgtaattgaa ttgttgtgcc atgaaaatcg tgaaacggag
     3181 ttttggagca gtaatcttcc tctagataca cctttacagg aaatacatga ttactttgct
     3241 aaacaacttg aaatttttcc attgttggat gaagaaattc atatgcgtat aactgaaatg
     3301 ggaaaacaaa acatactagt tgaacctgca gatgaagctt ctgcaaagag attagaagaa
     3361 attcgtactt atgtctcaga aaaagcaggt gtttgtttcc ctaatcatga tagatatcaa
     3421 ttccatattt caattgggta tcttcggatt cctctaaccg aagaggagga agaagagttt
     3481 actaaagtca gagcagaatt aactgaaatt ttattagaga agattccaac tattactgta
     3541 aaccgtattg actatactgt atttgaagat atgagacaat ttgttccata tcacgaaaaa
     3601 tttaaataaa ataataaaaa aggagaactt atcatgaaaa aaatgaaagt ttggtctact
     3661 gtacttgcaa cgggagttgc tcttactaca cttgctgctt gctctggagg ttcaaattct
     3721 acgactgctt cttcatctga agaaaaagct gataaaagtc aagaattagt tatctattcg
     3781 aactcagtct caaatggtcg tggtgattgg ttaactgcta aagcaaaaga agctggtttt
     3841 aatataaaaa tggttgatat cgctggcgct caattagcag accgtgttat tgctgagaag
     3901 aataatgcag ttgcagatat ggtatttgga attggtgctg ttgactcaaa taaaattaga
     3961 gatcaaaaat tactagtaca gtacaagcct aaatggttag ataaaattga tcaatcttta
     4021 tcagataaag ataattatta taatcctgtg attgttcaac cattagtttt aattggggcg
     4081 cctgatgtaa aagaaatgcc taaagactgg acagagttag ctagtaagta taaaggtaaa
     4141 tattcaattt atggtcttca aggtggtaca ggacgggcaa ttctagcaag tatcttagtt
     4201 cgttaccttg atgaaaaagg agatttaggc gtatcagaaa aaggatgggg agttgcaaaa
     4261 gaatactttg caaatgccta tactcttcaa aaaggtgaaa gctcagttgt taaagcgtta
     4321 gataaagaaa gcccaattca atacggtatg atctggggtt ctggagcgtt aatcggacaa
     4381 aaagaacaaa atgttgaatt caaagttatg actcctgaga ttggtgtacc atttgtaact
     4441 gaacaaacta tggttttaag cactagtaaa aaacaagcgt tagctaaaga atttattgat
     4501 tggtttggtc aatcagaaat tcaagtagaa tatagtaaaa aatttggatc tattcctgca
     4561 aataaagatg ccctaaaaga gttacctgaa gatacgaaaa aatttgttga tcaagtgaaa
     4621 ccacaaaata ttgactggga agctgttgga aagcatttgg atgaatgggt agaaaaagct
     4681 gaattagaat acgtaaaata agaaataaga gttatttttt atgtccaagt ctctctatta
     4741 tttctaaatt taaggaagaa ataaagagag gcttttggtt tagtaataga gggagttagt
     4801 gagatgatta aatttgataa tattcaaatt aaatatggtg attttgttgc aattgataat
     4861 ctgaatttag atatacatga aggggaattc tttacatttc ttgggccttc aggatgtggt
     4921 aaatcaacta ctttgagagc attggtaggt tttttagatc catcatcagg aagtattgaa
     4981 gttaatggaa cagatgtcac tcatttggaa cctgaaaagc gtggaattgg tattgtattt
     5041 caatcttatg cgctatttcc aactatgact gtttttgata atattgcatt tggtttaaaa
     5101 gttaagaagg tagctccaga tgttattaaa gctaaagtat cagcagtggc agcaaaaatt
     5161 aagatctctg atcaacagtt acagcgtaat gtatcagaat tatctggggg tcaacaacaa
     5221 agggtagcat tggctcgtgc tctggttctt gaacctaaaa ttctttgtct agatgaacca
     5281 ttgtcaaacc ttgacgcaaa attacgtgta gatttgagaa aagagttgaa aagacttcaa
     5341 aaagagttag gtattactac tttatatgtt actcatgatc aagaggaagc cttgacttta
     5401 tctgatagaa ttgcagtctt taacaatgga tacatcgaac aggtcggtac accagtagag
     5461 atttatcata attctcaaac tgaatttgta tgtgatttta ttggagatat taatgttttg
     5521 accgatgaaa cagtccacga agtattactg aaaaatacaa gcgttttctt agaggataaa
     5581 aaaggttaca ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca agattttatt
     5641 ctaaaaggga caattattga tgttgagttt tctggagtta caattcacta tacaataaaa
     5701 gtttctgaaa gtcagattct taatgtaaca agtattgata gtcaggctgc tattagatct
     5761 gtcggagaaa gtgtggaatt atttatcaca ccatcagacg ttctgcaatt ttaaggaggt
     5821 gcagtatgcg tcataaatta aatttaaaag attggcttat tcgtttaggg ttaatctggt
     5881 tcttagtaac atttattatt tatccaaact ttgatctagt agtgaatgta tttgtaaaag
     5941 gaggagaatt ttcccttgat gctgtacatc gtgttctaaa atctcagagg gcacttcaga
     6001 gtattatgaa cagttttaag ttagcatttt cactcattat tacagttaat gtcgtaggta
     6061 ttctttgtgt tctatttaca gagtactttg atattaaagg tgctaaaatt ttaaaattag
     6121 gttatatgac ctctttaatt tatggaggag tggttttagc gactggatat aaatttgtct
     6181 atggtcctta tggattgatt acaaaatttt tacaaaatgt tatcccttct ttagactcta
     6241 actggtttat tgggtatggt gcagtcttat tcattatgac attttcagga actgctaatc
     6301 atacattgtt tttaacaaat acaattcgaa gcgttgacta tcacactatt gaggctgctc
     6361 gaaatatggg agcaaaacca tttactgttt tccgaaaagt agtgttacca accttaattc
     6421 caactctatt tgcacttact attatggttt ttcttagtgg tttatctgca gtagcagcac
     6481 ccatgattgt tggtggtaaa gaatttcaaa ctataaatcc aatgattatt acatttgcag
     6541 ggatggggaa ttctcgtgat ttagctgcct tacttgcaat tattttaggt attgcaacta
     6601 caattttgct tactatcatg aataagatag aaaaaggtgg aaattatatt tctatctcta
     6661 agactaaagc gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc
     6721 acattgtagc atatggattg ttcacagttt tcatgcttcc actaattttt atagtattat
     6781 actcatttac agatccagtt gcaattcaaa caggtaactt aacattatca aactttactt
     6841 tagaaaatta tcgcttattc tttagtaata gtgcggcatt ctctccattc ttggtcagct
     6901 ttatttattc tattattgct gcgacaacag caacaattct cgcagttgta tttgctcgtg
     6961 ttgtcagaaa acataaatct cgttttgatt tcttatttga atatggtgct ctacttcctt
     7021 ggttactacc aagtacactt ttagcagtaa gtttattatt tacttttaat cagccacaat
     7081 ttcttgtctt gaatcagatt ttggtaggta gtttggtaat tctacttatt gcatatatag
     7141 ttgtaaaaat cccattttct tatagaatgg tacgtgctat tttatttagt gttgatgatg
     7201 agatggaaga tgcagcaaga agtatgggtg cttcaccttt ttatactatg atgaaggtta
     7261 tcattccatt tattttaccg gttgttctct ctgttattgc tttaaacttt aactctttat
     7321 taactgactt cgacttatct gtattccttt accatcccct agctcaacca ttaggtatta
     7381 cgattcgatc tgcaggtgat gaaacagcaa catctaatgc acaagctctg gtatttgttt
     7441 atacaattgt tctgatgatc atttctggaa cggtattata cttcacacaa agaccggggc
     7501 gtaaagtaag gaaataatca tgacagccac tagtcttggg ttatcaaata ttgaaatagt
     7561 tgtcaggatt gttttatcag tagtcattgg tagtataatt ggtttagaga gagggagcaa
     7621 atcccagcct gcaggcatcc gaacttatag tattgtttgt ctagctgcat gtttgattat
     7681 gatgacgaat gaatacgtat cttataaatt tgggacagga gatcctacac gattaggagc
     7741 tcaagttata tcaggtgtgg gttttctagg cgctggaacg attcttatta cagataaaaa
     7801 gaaaattaca ggtctgacaa ctgcagcagg catttgggct tcggcaggaa ttggattagc
     7861 tattggagta ggtttttatg agggagctct tttagtagcc atttctgttt ggggtgtgat
     7921 atccatgttc caaccactaa aaaaatatct gcaaaatcgt tctaaaatga ttgaattgta
     7981 tatagtggtt aaatctacag aagcctataa tcgtgtattg gtttattgtg cagaaaatgg
     8041 tatcagaatg accgattcaa gaacagcgtt tggagatgtt aattctgaca gaattgaata
     8101 ctttgatgtt ccggacaaaa aaatagcgtc atttattact ctggaattat caggtagatt
     8161 tgaacatctt cgactgatgg aagaaattgc taatattgta ggtgttattt atgtcgaaga
     8221 aattagctga aatttagtaa acaaagggac agatgttttt gtccttttgt tttttagatg
     8281 ttttaatcat ctaatttttg agtagatttt tgctcagagg tttttatgaa agattataaa
     8341 atcaattttg atttaggtaa aatagaatat tttgataata attgtttgat acaagtatac
     8401 aaatttattt ctttttatga tatttgtgaa atggtgtttg cctttcattt acctcctgat
     8461 gagttgataa caaatgttat
//
LOCUS       DAVKFH010000079         8266 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKFH010000079 DAVKFH010000000
VERSION     DAVKFH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.079"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            432..614
                     /locus_tag="VW006_001891"
                     /pseudo
     CDS             432..614
                     /locus_tag="VW006_001891"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1226..1510
                     /locus_tag="VW006_001892"
     CDS             1226..1510
                     /locus_tag="VW006_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380688.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1503..1741
                     /locus_tag="VW006_001893"
                     /pseudo
     CDS             1503..1741
                     /locus_tag="VW006_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1837..2055
                     /locus_tag="VW006_001894"
     CDS             1837..2055
                     /locus_tag="VW006_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380689.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2422..4746)
                     /gene="pflB"
                     /locus_tag="VW006_001895"
     CDS             complement(2422..4746)
                     /gene="pflB"
                     /locus_tag="VW006_001895"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9380690.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5140..6201
                     /gene="dinB"
                     /locus_tag="VW006_001896"
     CDS             5140..6201
                     /gene="dinB"
                     /locus_tag="VW006_001896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9380691.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(6348..7193)
                     /locus_tag="VW006_001897"
     CDS             complement(6348..7193)
                     /locus_tag="VW006_001897"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9380692.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            7448..>8266
                     /locus_tag="VW006_001898"
     CDS             7448..>8266
                     /locus_tag="VW006_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9380693.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIETTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILA
                     EIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKVC"
BASE COUNT         2683 a         1585 c         1548 g         2450 t
ORIGIN      
        1 ttattcaaga tacatactcc tagaataaac tttatatgaa attctcattt ttgtttttac
       61 aattctcctt agttaaatct tgtttaatat atgttttaca tatagtattt agcgccacat
      121 agtactgaac tctctccaaa aacggttatt cctctttgaa tagggcgtta tcacaagaaa
      181 agcatctcca cgtttcaact tcatatggct caaaaacaat caattgatgc taaaacctgt
      241 acctagatgt ttcggttcat aaaaccatga aactgtaaaa gtgaatgaaa ttgatagcga
      301 tagtcaaatc aagaggcatc ataactctaa aaagtcacaa tatataagtt catcctcgga
      361 aaaatatcat tctaattgtt gaaatgccta catgaaaaga aacgtcaaat gctcatgaaa
      421 caacgaatac aggtatcaaa actatgacaa aacaagtccc taaatttact aaagacactg
      481 ctcaacttta cacctgtaag tggttgttgt ataataaagt tacaaagatg tacgaccaca
      541 ctgttgtaaa tcatagtgtt cgcgaatata ttactgatag catttctaca aatacaattg
      601 aagagactgg atagacttta aacgaaatat gtcagtgttt tggcattcct agccttcata
      661 tcatttaaag aattctatag acaaaatttt tcccaataca gacactcgta acaactgctt
      721 catttttcta ccaacatatt taggaacagg ataagataca agaggattaa tccatagatc
      781 agttctatac caatctaaga caaataagct aaaaaaacga ttgataataa gcaaatagat
      841 tccaaatttt ctctatctgc tcattttaat aaacaatact agtgtaacta tccttccagt
      901 cagaaacttg tcaaatcaca ccgaaaactc ttctaaaatt tatctcgtta ggcaatcaag
      961 caaaaactcg acgatagtac aaacattatc atacagaatt gactgcctaa attatatact
     1021 ttagtaaggt tttcggataa gaaaaaaggt tcattttaca tttctaaaca ttcttttcta
     1081 agatgaaaaa cagaattttt cgattgtgat ttaaagcaac aagaagattt tcagtatcat
     1141 cctatagata cgagctaatt aagaaaaact acatttttga atataaacta caataatata
     1201 aactaaattt tataggagga agacaatgga ttggtacgat tatatgatac aggcatccaa
     1261 acaatcacaa ttcaacgcaa gccattggtt tcgctatttg cgaaaagtta tttttgaaga
     1321 ctattcttat ttaacaaacc aagatgtaga aaagttgcta gactccaaag aactaacccg
     1381 ttttcaaaaa attagcttga agtatgcctt tcaagagcat actccaactc ataaatatgt
     1441 gatttcatta aataaacctg ctaagttaac caatgttcaa aaattgatgg agaaatacaa
     1501 acatggataa aatgaaaccg gtcttccaag ccctaaataa gaaattaatt caggaaaatc
     1561 tgactttaac aattatggtt atgtcttaga atatcatggt ttacgtacca cacaagatgt
     1621 tgatgctttt tatgaccaaa atcagaaaat caatgaaatt attgctcgtg taggaaaaca
     1681 gtttaatctt aacatccacg aagagttatg gctcaacaat catgttgcaa agcaaatatg
     1741 aacaaacaac ctcccttaag tttatgcgaa tccttgtact catttgagaa tctgaccgtt
     1801 ttagtagtcc ctattgagta tgtgttagga atgaagatga tgagtatcag agagcaggat
     1861 ttgcaagata ttggtgcaat tattaaatac aaaaactttc attcaccatt cgataccttt
     1921 aaatatttaa aagacatggg atttgatact attgacctct ctgttctatt agaaggtttt
     1981 agttatgctt atggtatgga ttggttagaa aaatttttta aagaaaatca agataaactt
     2041 cgcgaatttt attaacagta tcaaacaaga tgattgtcaa ttagccagtc atcttatttt
     2101 tatgatgaaa tgaaataaaa tcggaacaaa tcacttagag aattccagtc aatttctagc
     2161 actgtttgag ccgtttcagc ggactatagt aaaatgaaat aagaatagga caaatcgatc
     2221 aggacagtca aatcgatttc taagaatgtt ttagaagtaa aggtgtacta ttctagtttc
     2281 aatctattat atttcagatt cggtccacta ttccttccca aaaacattgc atattgcgcc
     2341 gaaggaattg ctcatactat cccaagcaca aaaccctcag tcgtttgacc gagggttcct
     2401 tttcattatt caaaaacttg attagctcaa tgcatccaag gcatcatcca ttgaaagaac
     2461 ttcgtggaag acacgttgtg tcaattcagt tttttgttct ggagtgaggt atttagtatt
     2521 tacacagtat ccagagatac gtacgataac gtcttcacct gacataattt tttcgtaaac
     2581 atcgttcaag tccataacgt tcaagttaac gtgttgtcca ccgttttcga agtaaccatc
     2641 aaggattgtt accaagttat caacttgttc atcacgagtc ttaccaagag cgcgaggtga
     2701 tacttgtgta gtcaatgaga taccgtcagc tgcataacta aagtcaaggc tagaaagtga
     2761 gttcaagttt tgcaaccaac cacctttagc tttgttagat gggttagcac ctggtgagaa
     2821 gaattcaagt ttagacaagt tcacagaacc atcttcgttg aggtatacac ctttgtgaac
     2881 tggtgagtta ccagtttgtt tagagtaagc aacgttagat gtgattgtca aaagtgatac
     2941 tgtagcttct gcgtctttgt atagtttgtg gctacgtaga cgagttgtgt aagcttcgat
     3001 caaccattct gccaattcgt ttgaacgtgg gtcatcttca ccccagcgtg ggtagtcacc
     3061 gattgtttcg taatcgtaga tgtagccatc ttcgtcacgg attggtttaa ctgtagcgta
     3121 tttgatagct gacaatgtat caacagtgtt agcaaatcca cagataccga atcccatgtt
     3181 ggcacgttgt ttagttggca agaaggccat ttgaacagct tcgtagttgt acctatcagt
     3241 catgtagtgg atgatgttca aggcatctac gtaagtgtca gtcaaccagt caagagattt
     3301 ttcaaagttc gctttaactg attcgaattc aagaacttcg tcacggattg gttcgatatc
     3361 aaatactttg tagtctttgt gaacatcgtc gtaaccacca ttcaaaccag taagaagggc
     3421 tttaagaacg tttacacgag caccgaagta ctggatgttg tggcgttgtt cttcattttc
     3481 tggatcaagt ggagacacac agcatgagat acagctcatt tcaccatatc cgtctttagc
     3541 cattgttgtt acaccttcgt attggataga agagtgtttg tggctcatgt gcatacagta
     3601 gcgacggaag ttgtatggca atttgtcagt ccaaagaact gtcaagtttg gttctggtga
     3661 gttaccgatg ttgtcaagag tgttcaagaa acggtagtcc atcttagtaa cacggtgacg
     3721 accgtcgtta cccataccag ccatagaagt tgtgataaag gttgggtcac ctgagtacaa
     3781 ttggtcataa gcttttgtac gagcaaattt aactgtacga agtttcataa cgaaatcatc
     3841 aacgaattct tggatttctg attcagtaaa tgtaccacga gcaaggtcac gttctgcaaa
     3901 gatgtccaat acgattggta cacgacctag agatgtagca gcaccgttaa tcacacggca
     3961 gacagccatg aaagcaatgt taacccattg gattgcttct ttcacgttca tcgctggttt
     4021 gcgaacatca accccgtaaa ggtcacccag gcgaacaact tgttgcaatg cttggtattg
     4081 aaggtttact tcttcacgaa gacggattgt ttcttcatcg atttctttga ttgcattcca
     4141 gtcatttact ttttcttgca tcaagtagtc tgcaccgtaa agagcaagac gtgcgtaaac
     4201 accgatgata cgtccgcgtg agtatgcatc tggaagacca gttacagtgt gtgcgtgacg
     4261 agcgcgacga atatttgaag tgtaggcacg gaaaataccg tcgttaactg ttgttacata
     4321 tttagtgaag atttcgtgaa cagctgggtc tggttcgtat ccattttctt tcaaagtagt
     4381 ttcagccata cggataccac cttttggcat gaagttcaat ttgaagagtt catcgttttg
     4441 gataccgaag ataacttcat tttctttgtc gataaatcca gcagggatat cagcgataga
     4501 tgttggacga gtgtccattg ggaaacgagt ttcttcgtag tgtgctttag tttcttctac
     4561 aattttcttg atgtgaagtg aacgctctgt tggtcctgca aggaagcttt cgtctccatc
     4621 ataaggtgtg tagttagctt gtacaaagcg tgatacactt gctttttctt tccaatctac
     4681 gcctttgaag ccttcccaag ctttgtcaaa aatatcttgt gcttcaacaa ctgtcttaac
     4741 aaccatatta atgtcctctt ttttctagta acagtcatct gttacattca tgagacaagt
     4801 ataccatata gtaaccgatt ttaacaagtg aaaaatccct atttttacac tttcttttct
     4861 aaaacagtct atattttatt tcaaactgta ttatattttt gaaaaaataa agtccttttt
     4921 tctttttttc agaaaaaagg gtataataaa agaaaataag cagtaacact caatggaaat
     4981 cgaaaagcaa actaggaagc tagccgcagg ctgctcaaag cactgctttg aggttgtaaa
     5041 tagaactgac gaagtcagct caaaatactg ttttgaggtt gcagatggaa gctgacgtgg
     5101 tttgaagaga ttttcgaaaa gtataaaaag gtgctaggca tgttgatttt tcctttgcta
     5161 aatgatttgt caagaaaaat catccatatt gacatggatg ccttttttgc tgcggtggaa
     5221 atcaaggata atcctaaact cagaggaaaa cctgtcatta ttggaagcga ccctcggcaa
     5281 acaggtggac ggggagtcgt ttctacctgt agctatgagg ctcgagcttt tggtgtccat
     5341 tctgccatga gttccaagga agcttatgaa cgttgtcccc aggccgtctt tatctcagga
     5401 aattatgaga aatacaaagc tgtgggactc cagattcgag ctatttttaa gcgctataca
     5461 gatttgattg aacccatgag cattgacgaa gcctatttgg atgtgacaga aaataaactc
     5521 ggtatcaagt cagcggtcaa aattgctcgc ctcattcaaa aagatatctg gcaagaactc
     5581 catctaactg cttctgccgg tatttcctat aacaagttct tagctaaaat ggcgagtgat
     5641 tatcaaaaac cacatggttt gacagtgatt ctacctgaac aggctgagga ttttctcaaa
     5701 caaatggata tttccaaatt tcatggagta ggaaaaaaga cagtagaacg tcttcatcaa
     5761 atgggcgttt ttactggtgc tgatttactt gaagttcctg aggtaaccct aatagaccgt
     5821 tttggtagac taggctatga tctgtatcga aaggctcgtg gcattcacaa ctctccagtc
     5881 aaatccaatc gcatccgtaa atcaatcggc aaggagaaaa cctacgggaa gattctcctt
     5941 gctgaggaag atatcaaaaa agagctgact cttctatcag aaaaagtcgc tctcaatcta
     6001 catcaacaag aaaaagctgg aaaaattgtc attttgaaaa tccgctacga ggacttttca
     6061 actcttacca aacgaaaaag tcttgctcaa aaaacacaag atgctagtca gataagccaa
     6121 atagccctgc aactctatga agaattaagc aagaaagaaa gaggtgtccg cctattgggg
     6181 attaccatga ctggatttta aagctcaatg aaaatcaaag agcaaactag gaggctagcc
     6241 gcaggctgct caaaacactg ttttgaggtt gcagataaag ctgacgcggt ttgaagagat
     6301 tttcgaagag tataaaacct tgaagagttg ccccttcaag gtttttctta tacaaataaa
     6361 cggacaaaac tgtaaagtag caaaacacta ccaagcacga tacggtattt accaaaaagg
     6421 gtgaagtcgt gttttttcac atagctggtc aagaagcgaa tagccaccat gctgaccgca
     6481 aaagctactc ccatcgcgac caagagcaaa aacaattgcc caaagctcaa gagttctccg
     6541 gctttcacaa atttgaaaat ctttaaggca ctagctccaa acataacagg aatcccaaga
     6601 tagaaggtaa attctgtcac aactgaacga ctggttccat ttaacaaacc accgacaatc
     6661 gttgcacctg aacggctagt ccctggtaaa agagcaagaa cttggaagag tccgatatag
     6721 aaagcggtcg tataaggaag cttgtccaac tctgttacac ttggctcgat agcacgcgct
     6781 ttattgcgct tttccaaata gatgaaggca accccgtaga taatcaacat gagagcaact
     6841 gaaaccatgt tatggaagtg ggtatcaaac caatcatcaa atttaaagac accaagtaaa
     6901 ggtaaagtag caatcaagac cttcaaccat agtctccaag tcttacgaac ttcctgtttg
     6961 tccttagtcg gtttaaaagg attgagcttg ttaaaataaa tcaccataac tgctaaaata
     7021 gcaccaagct gaatcacgac attaaacatg gacataaagg cttcattttg attttggtat
     7081 tggatgaatt cctctgctaa aatcaagtga cctgtactgg aaatcggcaa ccattccgta
     7141 attccttcaa caatcccgaa gaagatagat tttaaaattt caataagata catagattac
     7201 tccctatatt ataattagcg cagttagcta ctgccaaagc ccaagtggta tacttggaat
     7261 aagccactgt ggattagttc atttcctcca atcgcttgac gatttccgaa aggatctaac
     7321 cacagctttt gtttcaattg gtaatgagtt tgataacgca tagacgtttt atctcgtcta
     7381 aggtaacaag gcaaaaagtt cgtggataac caagtagact tgaaatacta tatctggagg
     7441 tactcccatg ctttatgtgg gcattgatat cgctaaaaat aaacacgatg ttacagcctt
     7501 gaatgttcca ggaaaaactg ttcttaaacc actcactttt tcaaataata aagctggttt
     7561 tgaactctta gatctgtctc ttcgacagct caaccaagac tgtctcatcg ctcttaaact
     7621 tctttctgat cccaatcgtg aacaatttca acacgataat cggcaagtag acctaaaaat
     7681 actggctaga catattcatc gtctcaagaa aaaacagtct gattggaaag tacaatacac
     7741 tcgttgtctt gatatcatct ttcctgagtt ggataaaatc gttggaaagc attcagaata
     7801 tacctaccaa ctcttgacgc gctaccctaa tcctcagaaa aggattgagg caggatttga
     7861 taagctgata gaaattaagc gattgaccgc ttctaaaatt caggatatcc tctcagtcgc
     7921 acctcgttct atcgaaacaa catctcctgc tcgtgaattc gaaatcatcg aaatcatcaa
     7981 acattacaag aggctcattg acaaggcgga aacatgtgtc aatgacttga tggctgagtt
     8041 caactcagtc atcacgacgg ttactgggat tgggggtcgt ttaggggcgg tcattttagc
     8101 cgagattcga aatattcatg cctttgataa tcctgctcaa ttacaagctt tcgctggact
     8161 ggattcttct atttatcagt caggtcagat tgatttagct ggaagaatga tcaaacgggg
     8221 ttcccctcat ctgcggtggg cactcataca agctgccaaa gtatgc
//
LOCUS       DAVKFH010000080         8076 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKFH010000080 DAVKFH010000000
VERSION     DAVKFH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.080"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(19..2565)
                     /locus_tag="VW006_001899"
     CDS             complement(19..2565)
                     /locus_tag="VW006_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9380694.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(2688..3128)
                     /locus_tag="VW006_001900"
     CDS             complement(2688..3128)
                     /locus_tag="VW006_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9380695.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(3103..3972)
                     /locus_tag="VW006_001901"
     CDS             complement(3103..3972)
                     /locus_tag="VW006_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9380696.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(4014..4742)
                     /locus_tag="VW006_001902"
     CDS             complement(4014..4742)
                     /locus_tag="VW006_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380697.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(4750..5229)
                     /locus_tag="VW006_001903"
     CDS             complement(4750..5229)
                     /locus_tag="VW006_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9380698.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(5240..5938)
                     /locus_tag="VW006_001904"
     CDS             complement(5240..5938)
                     /locus_tag="VW006_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9380699.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(5931..6197)
                     /locus_tag="VW006_001905"
     CDS             complement(5931..6197)
                     /locus_tag="VW006_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9380700.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(6263..7189)
                     /locus_tag="VW006_001906"
     CDS             complement(6263..7189)
                     /locus_tag="VW006_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9380701.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(7264..8070)
                     /locus_tag="VW006_001907"
     CDS             complement(7264..8070)
                     /locus_tag="VW006_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380702.1"
                     /translation="MKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYLREQ
                     IYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAVLRY
                     YQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYIVVG
                     TIVLLVVLGYVGMASFIQEGVFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVSMII
                     AHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
BASE COUNT         2442 a         1823 c         1402 g         2409 t
ORIGIN      
        1 tttttatttt aggcttattt atgcatttcc gtattgaaga acaactgctt cgactgcagc
       61 tttttcacgg ctaatcaagt caacacgtgc tgcaatttcc ttgattccca taccgatgtt
      121 acggctaaga gcaaggtcag aaagttgcgg ctcaaagaac tccttgtatt ccgccaagcg
      181 ttgctgagtc ttaaatacat gagcaggaag gataacaaag ctatcaaagc tcatatctcc
      241 tccaagggct gccttaatcc aagcccagtt ttcacgcgcc caagaccaag ctgttttctg
      301 agttgcttga tgagctagga attggtaata ccaagcagac aagtcctgtg gtttgaccac
      361 aaatttgtcc ttccaagaag taatcaagtt ttggatatta tccgcatctg tactgtatgc
      421 aagagctgct gccaactggc gtttaaagac agcatctgtt gcgtgagtat aagtatcaag
      481 ataaagtgct aacaagtctt tagtctcatg atgtttcatc tcattaatca gaacttgtga
      541 acgaatagct gctgggagtt ctgcaagatt ctccttgtgt gttgcgaaga tttggctagc
      601 gacttgacga gcttctgcat catttgagcg aatcatcatc gaaacagcca gctgacgaac
      661 caattcatcc tcatctgatt ctccgtcttt agcttcaaaa ccaagacggt catagttatg
      721 acgagccaat ttagcaacca gtcctttgaa ggctgtttca gcatccgttc cttcatcaat
      781 aaagcgctca agggctgaaa tcacttgaga aacagctgaa accaccagat aagactcttc
      841 cttagcaagt ttatcaagaa ctggaagcaa gtctgcataa gaaatgtgcc ctgcctcagc
      901 caacaaacga cgttcttgaa caatttgcag tttgcttgtg ttatcaagtg tctctagctc
      961 agcaagaaca gcttctaaca agtctccttg atagtcggta atatagtggg cagtattttc
     1021 agtgttgaga cgaagagctc cttcattttc agcaagaaga gctgcgtagc cagggatttc
     1081 gatactttca gtttcgagtg tatcaggcaa gcctttccag ttgctattga ggggcaccac
     1141 ccagagacgg ttcttgtctt cgttctcacc gatgaagaat tgtttttgtg aaatcttcaa
     1201 gacatcattt tcaactttga cagtaagaac tgggtaacca ggctgttcca accaagaatc
     1261 catgaaggct gcgacatcac gtcctgacgc ttgaccaagg gcatcccaaa ggtcactacc
     1321 aatggtgttg ctgtattggt gtttttcaaa gtaggcatgc aaacctttag caaaatcagc
     1381 atctcctagc caacggcgaa gcatgtgcat gagacggctt cctttggcat agacgatagc
     1441 gccgtcaaag agtgtattga tttcatctgg atgtttaact tcgacgtgga cagactgaac
     1501 gccatcagta gcgtcacgtt caagagcaag aggtactcca cctgtttgga aatcttcaaa
     1561 gatattccag cttggttcga tggtatccac acagacgtat tccatcatat tagcgaaact
     1621 ttcattgagc caaaggtcat cccaccattt catagtcacg aggttcccaa accattggtg
     1681 agccaattca tgggccacaa caagggcaac ttgttgacgg ctagcaaatg tagagttctc
     1741 atcgacaacc aagtaaactt cacggtaggt cacaagaccc cagttttcca tagcaccagc
     1801 tgagaagtca ggaagggcga tgtggagaga ttgaggaatt ggatacttaa ctccatagta
     1861 atcttcgtaa aactcgatag agcgaacagc gatatccagt gagaaatcaa gatttgaaag
     1921 tggatgtgct ttggttgagt agacacctac cagggtacca tttttagttt tagcggtcac
     1981 cccttgcaaa tcaccagcaa caaaggccaa caagtaagaa gacatgcgag gtgttgtctc
     2041 aaacttccag atacctgttt ccttacggtt ttcaacatcg atttctggca tgtttgacaa
     2101 ggccaattca ccttctgctt ggtcaaagcg aagagagagg tcaaaagttg ctttggcttc
     2161 aggctcatcc acacatggga aagcttcgcg cgcaaaatgg ctctcgaact gagtagacaa
     2221 gacctccttc ttgactccat caactgtata ataagaaggg taaatccctg tcatgttgtc
     2281 tgtaatttta ccagaaaagg caagaaccaa ttcaacttga ccagcctcag ccaattcgat
     2341 atgaagggct tcattgtcat ggtcaactgt aaatggacga gcttgacctg caacttctac
     2401 agaggtgatt tccaagtctt tttggtggag ggagatgcgg tcactctgtg cttgaccagt
     2461 gatggtcact ttcccagaaa aagtcttggt ctcacgactc aaatctaaaa ataaatcata
     2521 atgttcagga acaaattgct taataaaatg ttcaactgct tgcatagttt tctcctattc
     2581 taagtttaag agctgctgct cttcttcaaa caaacttatt atatcatatt ttgcttgaaa
     2641 aaaaaggatt ggacagcgat ttccaaaact ttcttccttc taatagtcta cagtgggtag
     2701 accttgcgaa attcttctaa aacaaccttg ctttcaggtg taaaagtcag ccctacttca
     2761 cccaaacagt ctgtgaaaaa gtcctcatga acctggcgcc aataggcaag tgatttgtct
     2821 ccctcacctt ccttaaaggc atgttgcgca gaaacttgat tgaagagttc aacagaaacc
     2881 tttgtaattt cgacaatgca gacagactga ttttgactat ctaaaatgat atcgaaggtc
     2941 cctacttggg gaagtggttc gtcttctagt acatagaggt cataggctga tgctgttgct
     3001 gtcttttctc ctttaaacac caaatccgct aaaagatctg gttcaactcc aaaagcccaa
     3061 gcatcgattt catctccgat caaaggattg atttgcttgt atttattcca catttcttgc
     3121 ggtatcatgg gtactccttt gtaatttttt actttcttct tttatgtgtt taagatgatc
     3181 tggatggtca atctctaaat caaaaatctc tggaatagaa ctgtagtgga taatgcactt
     3241 gatacccaac tgattcattt tttgtatgaa agaagtattc agatagcctg ctacagcaaa
     3301 atcaatcttg ttctttcttg ctttatcctg catatctctt agcatatcta acattattgg
     3361 actttccata tcatgccatt gactgtttct catagtcgca aaaacaaagg aagtcaaatc
     3421 attcattcca actacaatct ttgaaatgcc cgtttccagt atactagata agtcaaaata
     3481 cgctgacggt aattcaatca tcgttccgac tttcccagta aaaccctgct gacgcaatac
     3541 tgtaatagct tgttttaatt ggtcggcatc attgacaaaa ggaaagataa gagatagatt
     3601 gggattggtt tgataaactt ctgtaacgac atgtgcttca gcctgaaatt catccaaaca
     3661 cgccagtaaa cgcctggttc ctctatagcc aaacaaggga tgcccttcgt caaaaaactc
     3721 tctagtcccc actaaacaat tggcttctgt attcgttaat tcagaaaaac gataccaaac
     3781 ttcttcatct gagtacaggg agcagatagt ctctagataa tcttttacaa attgctgaca
     3841 acttgctaat atgatgtttt gattaagctc tctcaacaaa tattccccac gaatcatgcc
     3901 gacgtggtga aatagttgag gataaatttt ttcaagaatt ttttcgccac taagggcaag
     3961 ttgatttctc atagccattc acctcgtctt aaaagataca atatcatata taattatatc
     4021 aaaatttcct ctagttcaaa agcttcataa ggttcagata actcatatcc taaaaattct
     4081 gctacttttt tggaagcctc attatgagca tcccaaagtg gaaacatatc tctatttaaa
     4141 ctctctagaa tcattgcagc accaagcttt ttagcaaatc catttccttg ttcgtttggt
     4201 ctagttgcaa cttccacttc aactgcttta cggtaaacta accctgagga aatcccagca
     4261 atcagttcat tatttttaag aatcacaaag ccaaatccac cttttaaaac aaaatcctga
     4321 taggactcaa aatccccctg taaatcctgt gaccattctt ccgtagaaaa gcagttataa
     4381 atatgattat caataggcac aatattgtaa ccttcctcta aatgagtaac tagattattt
     4441 aaaaattcaa cttgaaaatt tgccttatct ttaaaagaat agcgagtaaa agaatttagc
     4501 cccacttttc gatgcagaaa atcttgccaa ttagtatttt ctgaaataat aatcttataa
     4561 tctaaaccat attttctaac aaaatcttcc caaaatctgg aatccaactc ccctgctagg
     4621 tatagaaaat tcccaacatc ataaaagctt gttgtcctat cattgtttcg gtagacagtt
     4681 ccaatttgag atttaagacc atagagaacc atatttttct tccatgtttt aaatagttcc
     4741 attatccctc tacttcacct tccaattcat gtaagaagtc ttgtccagtt ctggcaatcc
     4801 taataattca gacttaacct tcaagactaa tggcgatgca ttttcttctg taatctcttg
     4861 aatatccatc caaatatatc caagtgaatc attcgcacca tcagacacag cttccgaaat
     4921 cgtaacttga ggtgcactct cattcatttc aacatcatac aaggctatga catggtgaac
     4981 cataaaattt tttaactctt ccctgacgaa aacatcgtag attcgaggat tagagtagct
     5041 tctaacagta aatcccgtct cttccataac ttctctagtc agcgtttccg tcagtccttc
     5101 accaagttgc tgactgcctc caggtagatc ataccgatgt tgataagggc ctctcgtttt
     5161 ttcaatgcaa agtaactttc cattttcaaa gcaaacacag tagaccccaa agtgattttt
     5221 gatttccatc caactcctcc tacttcaaag accagccacc atctattgtc agaatttgtc
     5281 cctgcatggc agatgccttt ccactggcca aaaagagact gacttctgcc acttcctctg
     5341 gttcaatcca gcgcttgatt ggggtttcac tagccaccca gtcagctaag ccacctgtct
     5401 caaaatccgc agcggtcata gctgtcttga ctgctcctgg agcgatacca aagacctgaa
     5461 tcctagcttc agcatagtct agagccaact gcttggtgaa gccagccaag gcatgcttgg
     5521 atgaagtata ggcgtgacca cctccacctg ctaggctaga agcaatggaa cacatattga
     5581 tgatgattcc ctttttattt tccagcattt gtgtcaaata ataccgagtc aactctactg
     5641 gaataatgta gttgatttca aaaatctctt gaatgtcctg cgccgtttgt tccaacagtg
     5701 gtttgtaagc atccaaaact ccggcagtat tacacaaaac atccacctga gggcaccagt
     5761 caaaaatagg ttccaagtcc aaggtcaaat ctctctgtaa aaagtgaaaa tcaccctcta
     5821 agagtggctt ttcaccttgg tcaactccat aaacttgata gcccttctct aaaaagaggc
     5881 gagcttgagc caatccgatc cctgaactca ctcctgtaat gagtacacgt ttagtcatgc
     5941 acttctaccc aatccgttgc caaaacatca caaactgtcg ggctccacat ggaaaaacct
     6001 tctccttctc cagaaacgtt gattaggaaa taaggtgtca tttcaagtgc aagcccattt
     6061 tgctcgatgg tatcaaagag ttggacatag ttttccgcac ctccccaacc agttcgtaca
     6121 tattttctct tagcctttaa cccaggcagg atctcttcaa atgtcatgtt tttctccttt
     6181 aattctacat tcttcattta attatagcaa aaaaccactt tatacggctt tttgactgtg
     6241 agttattcaa acctgctact acttacggca aattattccc tgcagcaaga taaatttcat
     6301 accattcttt tcttgttaag ctaaagtttg ccgctcggct aacttctctc aagtgcttag
     6361 gatttgttgt acctacgact gcctgcattt ttgctggata acgcaatatc caagaaatgg
     6421 caatagttga agaggttact ccatatttaa tagctaaacg atcaagtact tgatttaaag
     6481 cttgaaattt ctcatttcca acaaagttcc ctttaaaata cccgaattgc aagacagacc
     6541 atgcttgaat gaccacatcg tgtaattggc aatattcaaa aatgctgcca tctcgcatag
     6601 ctgcttgact atcttccata ttaacatgaa aagctgattc aaatcctgga gtaaaagccg
     6661 cactcaattg tagctgatta acagctaacg gctgcttgac atctttttta agcaactcca
     6721 tcatcatagg attttgatta gaaactccaa aatctcgaac tttaccttgt ttataaagga
     6781 gatcaaaggc ttctgctact tggtcagatt ccatcaaagc atctggtcga tgaaggagca
     6841 agctatctag atgatcaatc ttcaatcttt gcaaaatacc gtctactgat tttataatat
     6901 agtccttaga aaaatcaaaa taggtaaatt cttcaatgcg aatgccacat ttggactgaa
     6961 tccacatctt ttctcttaaa tctggacgat tttttaggac aagacctaac agttcttcac
     7021 aacgaccacg accataaata tcagccaagt cgaaggcatt gattccaaca gaaagtgctg
     7081 tttctacaag ctcttcaact tcttttacag atttatcttt tattctcatc attccgagaa
     7141 caatttctga taattctttg tcatcttgac caagagttat gtatctcatc aaatttttct
     7201 cctttaattt ctaacattct tcccttcatt ataacaaaaa accgctttgc aacggctttt
     7261 tgactatact tcactccatt ttatcttctt aaacccacgg aacaagacaa agattccaat
     7321 aaagaggaca gctaaaggaa taacttttgt aaggaaaaca tttgaaattc ccatccactc
     7381 ataataacgg agcagagaac ccaccacaag atgggcaata atcatactga caaatggacg
     7441 aaagaccgct tctttccaat tccaaatacc gataactagc gaaatcgtaa agacagacaa
     7501 actatcccag ggagccggaa tataaaagac tccttcttgt atgaagcttg ccattcctac
     7561 atatcctaaa acaactagaa gaactatagt cccaacaaca atgtaagtgc caattttcat
     7621 tttaggagaa tcttggacta aacttcttcg taaaattgtg gccacaagtc caaatccaat
     7681 cagaaaaata agaagttgcc ctaaaaatgt gagcaaattg actgttaata gaggaccttt
     7741 agaaaaatca cttagtagtt gataataacg taataccgcc aggacaagaa ttggcgtcaa
     7801 aagggactct ttgatagaac tgcgaggtgc tcccttgaga atctctttca ttattttttt
     7861 aggattctta cctagataat cctctgcact catgccatct cgttctgctt ctgagaaatc
     7921 tagcatcatc aaatagatct gctctctgag atagtcttca tcatagagaa atccagcaag
     7981 attaaaactt tcccacaact cctcaaaata cttttgattc tcctcagaaa actcatgtag
     8041 caaagcgctt gtttcttcgt aatacttcat tttctt
//
LOCUS       DAVKFH010000081         8036 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKFH010000081 DAVKFH010000000
VERSION     DAVKFH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.081"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            53..1534
                     /locus_tag="VW006_001908"
     CDS             53..1534
                     /locus_tag="VW006_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9380703.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1896..2897
                     /locus_tag="VW006_001909"
     CDS             1896..2897
                     /locus_tag="VW006_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9380704.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     KVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3048..3965
                     /locus_tag="VW006_001910"
     CDS             3048..3965
                     /locus_tag="VW006_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9380705.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAVRLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3976..4866
                     /locus_tag="VW006_001911"
     CDS             3976..4866
                     /locus_tag="VW006_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9380706.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4895..6511
                     /locus_tag="VW006_001912"
     CDS             4895..6511
                     /locus_tag="VW006_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9380707.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6521..7840
                     /locus_tag="VW006_001913"
     CDS             6521..7840
                     /locus_tag="VW006_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9380708.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         2700 a         1265 c         1528 g         2543 t
ORIGIN      
        1 gtataattgc aagaggttta atccgataat ttataaagag agaaagatat tcatgagaga
       61 tttattatct aaaaaaagtc ataggcaatt agaattatta gaattattat ttgaacataa
      121 acgttggttt catcgttctg aactagcaga gttattaaat tgtacagaac gtgcagtcaa
      181 agatgatcta tcccatgtta aatctgcttt tcctgacttg atttttcatt cttctactaa
      241 tggtatacgc attattaata ccgatgatag tgatattgaa atggtttacc atcatttctt
      301 taaacattca actcattttt cgattttaga attcatcttc tttaatgaag gttgtcaagc
      361 tgagagtatt tgtaaagaat tttatatcag ttcatcttcg ctctatcgta ttattagcca
      421 aatcaataaa gtgattaaaa ggcaatttca atttgaagtc agtctgaccc ctgttcaaat
      481 cattggaaat gagagagaca ttcgttactt ttttgcacaa tatttttcag aaaaatatta
      541 tttcctagaa tggccatttg aaaatttttc atcagagcca ctatctcaat tgttagaatt
      601 ggtttataag gaaacaagct ttccaatgaa tttgtcaacg catagaatgc taaagttgct
      661 cctagttacg aacctatata gaataaagtt tggtcatttt atggaagtag ataaagattc
      721 ttttaacgac caaagtttgg attttttaat gcaggcagaa ggaatagaag gtgttgctca
      781 gagttttgaa tcagaataca atatctcttt agatgaagaa gttgtttgcc aattatttgt
      841 gtcttacttt caaaaaatgt ttttcataga tgaaagtctc tttatgaaat gcgtaaaaaa
      901 ggatagttat gttgaaaaat cttaccatct attgagtgat tttattgatc agatttcagt
      961 caagtatcag attgaaatgg agaataagga taatctgatt tggcatctgc ataataccgc
     1021 acatctgtat cgtcaagagt tgtttactga gtttatttta tttgatcaaa aagggaatac
     1081 aatcaggaac tttcaaaata tttttccaaa atttgtttca gatataaaaa aagagctttc
     1141 tcactattta gagactcttg aggtttgttc tagttcaatg atggtaaatc atctatctta
     1201 taccttcatc actcatacca aacatttggt gattaatttg ctacaaaatc agcctaaact
     1261 aaaggtttta gttatgagta attttgatca gtatcatgca aaattcgttg cagagacact
     1321 ttcttattac tgtagcaata attttgaact ggaagtttgg actgaattag aattatcaaa
     1381 ggaatcttta gaagattcac cttatgatat cattatttct aattttatta ttcctcctat
     1441 tgaaaataag agactcatct attcaaataa tataaacacg gtctcactca tatacttgtt
     1501 aaatgccatg atgtttattc gattagatga gtaaccaaaa ataacaaaaa aacgctattt
     1561 cgcgtttgaa ctgtacctca aaagttagac agaaaaaatc taatttttgg ggtgtttttg
     1621 ttaagaaaag accttcaatc atttaagcaa aatgtacatc tagatttata gccctctaaa
     1681 actcttgctt tttttgtaga agatacgaaa aaagccataa aaccaaggct tttcctgttt
     1741 tatgtagatg tttacacatg aaaattgaag tattttcaaa agctaattgg agtgatttta
     1801 ttcatgatgg gataaatgat ctggccaaaa atgattgaga aatagttgct taaaaagcat
     1861 ttgcatgata ctatatattg aaaggaggtg ataatatggt agaacaaaga aaatcaatta
     1921 ccatgaaaga tgttgcttta gaagcaggag ttagtgttgg aactgtttca cgtgtaatta
     1981 ataaagaaaa aggcattaaa gaagtaactt tgaaaaaagt ggaacaagcg attaaaactt
     2041 tgaattacat tccagattac tatgctagag gaatgaaaaa aaatcgaaca gaaacgattg
     2101 caatcattgt accaagtatc tggcatccct tcttttcaga atttgctatg catgtggaaa
     2161 atgaagtcta taagagaaat aacaaattac tcttatgttc tataaatggt acaaatagag
     2221 agcaagacta tctggagatg ttgcgtcata ataaagttga tggagtggtt gccattacct
     2281 atagtccaat tgaacattac ttgacgtcag gaattccctt tgttagtatt gaccgcacat
     2341 actcagatat tgccattcct tgtgtttcat cagataatga tgcaggtgga agagaagcag
     2401 ctaaacaatt aattagtaaa ggatgtcagc atttagcctt tgtgggcgga cataatacaa
     2461 ccattaatga aaccaaacgt cgtagaattt catttgaaaa gtatgtccaa gagcataaaa
     2521 tacctcttag tatttttgat ttggatgaga aagttactga ctattatgga aagctagaga
     2581 gtttcttgga agaaaattct tctatagatg gaatttttac aatcaacgat tttacagctt
     2641 tggatgtgat tgaaatacta gaaaaaaatg gcaaacgtat tcctcatgat gtccaaatta
     2701 ttggatacga tggaattaaa atggctggag atagagatta tctactttca actattgaac
     2761 aaccattgga agaaatggca aaagaagcgg ttcgtatttt gtttgatata attgatggaa
     2821 agactgttaa tttgcagaca atcctaccag taaaatttgt tgaaggaaaa acaacaaaaa
     2881 atgaaaataa gtcttgacag aaagcgctat caatgataga atgaattcag ataaaaagat
     2941 ttatttttaa aacaaaaatg aaacgtttca aaaaaagaaa taaaaagaca gcgccaagcg
     3001 ctatctttta tagaaaaaat gaaacgtttc aaaaaaagga ggttgctatg aatagcaaag
     3061 cgaagcaagt ttctctttgg gaaagaatca agaaacaaaa actcttgtta ttgatgactg
     3121 tccccggttt agttttaacc tttatcttta aatacatccc tatgtatggg gttttaatcg
     3181 catttaaaga ttacaatcct ttaaaaggaa ttttagggag tgattggatt ggtttttctg
     3241 agtttacaaa attcatatcc tctcccaact ttggtatctt gttagccaac acattaaaat
     3301 taagtatcta tggtttattg cttggctttt taccaccaat cattcttgcg attatgctca
     3361 atcaactctt gagtgaaaaa gtcaaaaaac gaattcagct cattttatac gcaccaaact
     3421 ttatctcagt cgttgttatt gtcggtatga ttttcctctt cttttcagtg ggaggaccaa
     3481 tcaacaattt tctttctatg tttggaatga aggctgactt cttgacaaat ccagacttct
     3541 ttagaccctt atacatcttt agtggtatct ggcaaggaat gggctgggct tcaacgctct
     3601 acacggcaac attggtaaat gtagatccag ccttagtaga agcagttcga ctggatggag
     3661 ccaatatctt ccaacgaatc tggcacattg atattccagc tcttaagcct attatggtta
     3721 tccaatttgt tttagctgca ggtggaatta tgaatgtcgg atatgaaaaa gcattcttga
     3781 tgcagacatc gttaaatttg ccaacttctg aaattatctc gacatatgtc tataaagttg
     3841 gtcttgtatc aggagactat tcttactcaa cagcggttgg tttgtttaat gcagtgatta
     3901 acgtagtatt gcttgttgca gttaaccaaa tcgttaaacg catgaataat ggtgaaggaa
     3961 tttaaggagg aaagtatgaa aaattcgatt atggatacaa aatttgatag acgtatctta
     4021 ctcttaaata aaatcattat tgtctttatc gttttgatga ctttgcttcc tttactttat
     4081 atcgtcgtag catcctttat ggatcctaag gttctggtta gtagagggat tagctttaat
     4141 ccagccgatt ggactgtaga aggttaccag cgtgtattca gtgaccaatc tattctaaga
     4201 ggttttatca attctctact atactctttt ggatttgcag ctttaacagt attgctatct
     4261 gtgtttacag cttatcctct ttctaagaaa gacttggttg gacgtcgttg gattaactac
     4321 ttcttgattg tgactatgtt ctttggtggt ggtttagtcc caacttactt gctcgtaaaa
     4381 gaattgggaa tgctcaatac tccatgggct atcattgttc caggtgctgt caatgtttgg
     4441 aatattattc ttgctagggc ctatttccaa ggattgcctg aagaattagt tgaagctgct
     4501 gtcattgatg gagcgaatga cttgcagatt ttctttaaaa tcatgcttcc tcttgcaaaa
     4561 ccaattatgt ttgttctctt cctctatgct tttgtaggac agtggaactc atactttgat
     4621 gcaatgattt atatcaagga tccaaacttg gaaccattgc aacttgtact tcgtaaaatt
     4681 ctcattcaga gccaaccagg tcaagacatg attggagcac aagcggctat gaatgaaatg
     4741 aaacgtttag ctgaattgat taaatacgca actattgtca tttccagctt gccattgatt
     4801 gttatgtatc cattcttcca aaaatacttt gataaaggaa ttatggctgg ttcacttaaa
     4861 ggataaaaaa agaaaaaata aaaggagttt tctcatgaaa ttcaaaacat tctcaaaatc
     4921 agcagttttg ttgacagcta gtttagcagt acttgcagcc tgtggctcaa aaaatacagc
     4981 ttcaagtcca gattataagt tggaaggtgt aacattcccg cttcaagaaa agaaaacatt
     5041 gaagtttatg acagccagtt caccgttatc tcctaaagac ccaaatgaaa agttaatttt
     5101 gcaacgtttg gagaaggaaa ctggcgttca tattgactgg accaactacc aatccgactt
     5161 tgcagaaaaa cgtaacttgg atatttctag tggtgattta ccagatgcta tccacaacga
     5221 cggagcttca gatgtggact tgatgaactg ggctaaaaaa ggtgttatta ttccagttga
     5281 aaatttgatt gataaataca tgccaaatct taagaaaatt ttggatgaga aaccagagta
     5341 caaggccttg atgacagcac ctgatgggca catttactca ttcccatgga ttgaagagct
     5401 tggagatggt aaagagtcta ttcacagcgt caacgatatg gcttggatta acaaagattg
     5461 gcttaagaaa cttggtcttg aaatgccaaa aactactgat gatttgatta aagtcctaga
     5521 agctttcaaa aacggggatc caaatggaaa tggagaggct gatgaaattc cattttcatt
     5581 tattagtggt aacggaaacg aagattttaa attcctattt gctgcatttg gtatagggga
     5641 taacgatgat catttagtag taggaaatga tggcaaagtt gatttcacag cagataacga
     5701 taactataaa gaaggtgtca aatttatccg tcaattgcaa gaaaaaggct tgattgataa
     5761 agaagctttc gaacatgatt ggaatagtta cattgctaaa ggtcatgatc agaaatttgg
     5821 tgtttacttt acatgggata agaataatgt tactggaagt aacgaaagtt atgatgtttt
     5881 accagtactt gctggaccaa gtggtcaaaa acacgtagct cgtacaaacg gtatgggatt
     5941 tgcacgtgac aagatggtta ttaccagtgt aaacaaaaac ctagaattga cagctaaatg
     6001 gattgatgca caatacgctc cactccaatc tgtgcaaaat aactggggaa cttacggaga
     6061 tgacaaacaa caaaacattt ttgaattgga tcaagcgtca aatagtctaa aacacttacc
     6121 actaaacgga actgcaccag cagaacttcg tcaaaagact gaagtaggag gaccactagc
     6181 tattctagat tcatactatg gtaaagtaac aaccatgcct gatgatgcca aatggcgttt
     6241 ggatcttatc aaagaatatt atgttcctta catgagcaat gacaataact atccaagagt
     6301 ctttatgaca caggaagatt tggacaagat tgcccatatc gaagcagata tgaatgacta
     6361 tatctaccgt aaacgtgctg aatggattgt aaatggcaat attgatactg agtgggatga
     6421 ttacaagaaa gaacttgaaa aatacggact ttctgattac ctcgctatta aacaaaaata
     6481 ctacgatcaa taccaagcaa acaaaaacta gaggttgatt atgggagata agaaatacac
     6541 agtagaaaaa gccaatcgtt ttatagcaga aaataaacat ctcgttaata ctcaatataa
     6601 gcctgaagaa catttttcag ctgagattgg ttggatcaat gatccaaatg gatttgtcta
     6661 ttttcgtgga gaataccatc tcttttatca attctatcca tatgatagtg tttgggggcc
     6721 tatgcactgg ggacatgcta aaagtaagga cttggtgact tgggagcact tgccagtggc
     6781 acttgctcct gaccaagatt atgaccgaaa tggttgtttc tcaggctctg ccattgtcaa
     6841 ggatgatcgc ctctggctca tgtacactgg acatatcgaa gaagaaaccg gtgtccgcca
     6901 agtgcaaaat atggcatttt cagatgacgg gattcacttt gaaaagattg aacaaaatcc
     6961 agttgcaact ggatcagact tgccagatga gttgattgct gctgattttc gtgatccaaa
     7021 actctttgaa aaagatggac gctattactc cgtagtagct gccaaacaca agaataatgt
     7081 gggctgtatc gttctactag ggtccgataa cctagtagaa tggcagttcg aatccatctt
     7141 tttaaaaggg gtagaacacc aaggctttat gtgggaatgc ccagattact tcgagttaga
     7201 tgggaaagat tgccttatta tgtcacccat gcgttatcag cgtgagggag actcatatca
     7261 taacatcaac tcatcgcttt tgttcacggg taaggtagat tggggagaaa aatgttttat
     7321 cccagaatca gttcaagaaa ttgatcatgg ccaagacttc tatgcgcctc aaacattgtt
     7381 ggacgatcaa aatcgtcgta tcctgattgc ttggatgcag acatgggggc gtacccttcc
     7441 aacccatgac caagaacaca aatgggcatg tgccatgact ttacctagaa ttttaagatt
     7501 ggaagatggc aaactaagac aattccctgt taaaaaaggc caatatcaaa tccaaataga
     7561 taaagattgt cattaccact taggaaatga tatagattat cttgaatttg gttatgatag
     7621 taatgcgcag caagtttaca ttgatcgtag ccatcttatt caaaaaattc taggtgaaga
     7681 agaacaggac actagtcgac ggtatgtaga tattgaagct aaagaattgg aagttgttct
     7741 agataaaaat tccatcgaga tttttgtcaa tcaaggtgaa gcaagcttga ctgcaactta
     7801 ttacttaacg gtgccagctg agctatcacg aattgattaa aaattaagtt atttctccta
     7861 aagaaaaagt tctctttcta aaatagtgga aagaggactt tttgtgtttt gggtatataa
     7921 gcttagttta tggtatttgt aaaattggtg ttggattatg atttaagcta gttttctaaa
     7981 gaatttgaaa aaaattttat ttaagcaaaa aaaacccttg gttccaaggc ttttcc
//
LOCUS       DAVKFH010000082         7963 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKFH010000082 DAVKFH010000000
VERSION     DAVKFH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.082"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(164..304)
                     /locus_tag="VW006_001914"
     CDS             complement(164..304)
                     /locus_tag="VW006_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEU9380709.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQVTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            473..1015
                     /locus_tag="VW006_001915"
     CDS             473..1015
                     /locus_tag="VW006_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9380710.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            1015..1644
                     /gene="nth"
                     /locus_tag="VW006_001916"
     CDS             1015..1644
                     /gene="nth"
                     /locus_tag="VW006_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9380711.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            1855..2376
                     /gene="pyrR"
                     /locus_tag="VW006_001917"
     CDS             1855..2376
                     /gene="pyrR"
                     /locus_tag="VW006_001917"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9380712.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            2395..3318
                     /locus_tag="VW006_001918"
     CDS             2395..3318
                     /locus_tag="VW006_001918"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9380713.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            3368..4447
                     /gene="carA"
                     /locus_tag="VW006_001919"
     CDS             3368..4447
                     /gene="carA"
                     /locus_tag="VW006_001919"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9380714.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            4759..7935
                     /gene="carB"
                     /locus_tag="VW006_001920"
     CDS             4759..7935
                     /gene="carB"
                     /locus_tag="VW006_001920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9380715.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         2306 a         1612 c         1798 g         2247 t
ORIGIN      
        1 acaggtccat agagacttag tacgtgtttg acatactcgt aatggatttt atcatagttt
       61 cgccaagcag tgcgtgcttg attgtttagt tttaggctac ccagtccaat cagaggactg
      121 gtgggttggt aaaatttctc aagattaatt aagatactgt tgttcaactt ttctgttttt
      181 tgagctcttg catagtagtg tttagcagct cttgtagtcg aatatcatct gccgtaacct
      241 gtttacagtt ttggtagctt ttatttaact tggataggag ttgaatgagt ttcttgtctt
      301 ccatgtgagc atcctccttg atctgatgta ctctttccct atagtatagc actaaaaaat
      361 aaaactatca tagtaaatat gttaaaattt gaattttaat tacaaagttt ggtgtgctga
      421 tttgtagcct tttcatggta taatcaagag agtaaatctt tatggaggaa gtatgaaatt
      481 aaatattcaa gaaattcgta agcagtctga aggtttgaac tttgaacaaa cgttagattt
      541 agttgatgac ctgcgtgcac gtaatcaaga aattttagat gtaaaagata tccttgcagt
      601 tgggaaagta caatatgaag accgtatgta tttcttagat tatcaactat cttataccat
      661 tgttcttgct tcgagtcgca gtatggagcc agttgagtta gttgaatctt atccagtcac
      721 ggaagttttc atggaaggcg caactaacca gctagatcaa gaagttttag atgatgactt
      781 ggtcttgccc atcgaaaatg gggcgcttga ccttgctgag agtgtatcag acaatatcct
      841 gctaaacatt cctatcaagg tcttgacggc tgaagaagaa gctggtcaag gatttgtctc
      901 aggaaatgac tggcaaatca tgacagagga agaataccaa gctcaacaag cagtaaagaa
      961 agaagaaaac agtccttttg ctggcttaca aggactattt gacggagatg aataatggtc
     1021 ttgtcaaaaa aacgagcacg aaaggtgcta gaagaaatca ttgccctctt cccagatgcc
     1081 aagcctagtc ttgattttac caatcatttt gaactcctgg ttgcggtcat gttatcagcc
     1141 cagacgacag atgcagcggt aaataaggcc acaccaggtc tctttgttgc ctttccaaca
     1201 ccccaagcta tgtctgtagc gacagagagt gagattgctt cacatatttc tcgcttggga
     1261 ttgtatcgga ataaagctaa attccttaaa aaatgtgccc aacagttact agacgatttt
     1321 gatggtcaag tccctcagac acgtgaagaa ttggagagtt tggcaggtgt tggtcgcaag
     1381 acagccaatg ttgtcatgag tgtaggattt gggattccag cctttgcagt ggatactcat
     1441 gtggagcgta tttgcaaaca ccacgatatt gtcaaaaaat cagcgacgcc acttgaggtg
     1501 gaaaagcggg tcatggatat cttgccgcct gagcagtggt tagctgccca tcaggccatg
     1561 atttattttg gaagagccat ttgccatcca aaaaatccag agtgtgacca gtacccacaa
     1621 ttatatgatt ttagcaattt gtaagatgct acattgaaac tttttgcttg attttaagca
     1681 tgctttgtgc tatagtaaat gataactaaa tattccttta aacctgtccc gtgaggcagg
     1741 caaggagcgc gataaagtag aagggtgaag tctacactgt gtgcagtagg gctcgcttgg
     1801 ctataaattt caagtctcct tgtttaagga gacttttctt tttggaggtt tgtcatgaaa
     1861 acaaaagaag ttgtagacga attgaccgtc aaacgagcga ttacgcgtat tacttatgag
     1921 attatcgaac gcaacaaaga tttgaataag atcgtcttgg ctggtattaa aactcgtggt
     1981 gtctttattg cccaccgaat ccaagaacgt ttgaagcagt tagaaaatct ttcagttcct
     2041 gttgtggaat tggatactaa acctttccgt gatgatgtta aaagtggaga agatacttct
     2101 ttggtttctg tcgatgtgac agaccgcgaa gttatcttgg tggatgatgt gctctataca
     2161 ggtcgtacca tccgtgctgc tattgataat attgtcggtc atggtcgtcc tgcgcgtgtg
     2221 agtttagcag ttctagtcga tcgtggacat agagaattgc caatccgtcc agattacgtt
     2281 ggaaaaaata tcccaaccag tcgttctgaa gaaatcatcg tagagatgac agaacttgat
     2341 gaccaagaca gagttctgat tactgaagaa gcttagaaag ttaaaggagt agccatgtca
     2401 gaaaatcaac aagcattgaa ccatgtggtg tccatggaag acctcactgt cgatcaagta
     2461 atgaaattga tcaagcgagg aattgagttt aaaaatggag cccagcttcc ctatgaagac
     2521 catccgattg tttccaatct cttctttgag gattctacac ggacacataa gtcctttgaa
     2581 gtcgcagaga ttaaacttgg attggaacga cttgactttg atgtgaagac tagttcggtt
     2641 aataagggtg agacacttta tgacaccatt ttgactctgt ctgctttagg agtggatgtc
     2701 tgtgtgattc gccatccaga ggtcgactac tatagagaat tgattgctag tccaacgatt
     2761 acgacttcca tcatcaatgg tggagatggt tcgggccaac accctagcca gagcttgctt
     2821 gatttgatga ccatttatga ggaatttggc cactttgagg gtcttaaagt tgctattgca
     2881 ggtgacttgg atcactcacg cgttgccaaa tccaatatgc agattttgaa acgcttggga
     2941 gctgaactct ttttcgctgg acctgaggaa tggagaagtc aagagtttgc agactatgga
     3001 cagtttgtaa ccattgatga aatcattgat caggttgatg tcatgatgtt tctccgtgtg
     3061 caacacgaac gccatgatag tggagctgta ttttcaaaag aagactacca tgcccaacat
     3121 ggcttgactc aagaacgtta cgatcgtttg aaagaaacag caatcctcat gcacccagct
     3181 ccaatcaatc gtgatgtaga aatagcagac cacttggttg aagcaccaaa atcacggatt
     3241 gtccaacaaa tgaccaatgg tgtctttgtt cgaatggcaa tcttagaatc cgtactagcg
     3301 agtagaaacg ccaactaaaa cacaagacgt taaaagacgc cagtgagcgt ccacgagagg
     3361 tgaaaatatg acaaaaagaa ttctagtatt agaagatggc acagtttttg aaggcaaggc
     3421 cttcggagca gatattgatg taacaggcga aatcgtcttt aatacaggga tgaccggcta
     3481 ccaagaatcc attacagacc agtcttataa tggacaaatc ttgaccttta cttatccttt
     3541 ggtaggaaat tatggtatta atcgtgatga ttatgaatcc attattccaa cttgtaaggg
     3601 agtcgttgtt tttgaagaag cgcgtagagc tagcaactgg cgcaaccaaa tgaccttgga
     3661 tgaatttttg aaagccaaga aaattccagg gatttcagga attgatacgc gtgctcttac
     3721 caagattatc cgtaagcatg gtactatgcg tgcaaccttg acccatgttg gggacagtat
     3781 ggaccatgtg acggaccagc tccaagcaac agttttaccg acagacaata tcaaacaggt
     3841 ttctactaaa acgtcttatc cagctccagg agttggtttg agcgtggtgc tagtggactt
     3901 tggtctcaag cactcaatcc tacgtgaact ttctaaacgc aactgtaacg tgacagttgt
     3961 tccttattcg acaacggctg aagaaattct ccatctcaat cctgatggag ttatgttgtc
     4021 aaatggtcca ggtaacccag aagacgttcc acaagcactc gacatgattc gcggtgtaca
     4081 aggaaaaatt ccaatctttg gtatttgtat gggacaccaa ctctttgcaa tggcaaacgg
     4141 ggctaagacc tataagatga agtttggcca tcgtggattc aaccatgcgg tacgtgaaat
     4201 cgcaacagga cgagtagact tcaccagcca gaaccatggt tatgcagtca gccgcgagga
     4261 tttgccagag cacttgatta ttacccacga agaaatcaat gacaagtcag tggaaggtgt
     4321 gcgccacaga taccaacctg ctttctctgt tcaataccac ccagatgcag ctcctgggcc
     4381 acacgacgct agctacctat ttgacgagtt tatcgagatg atggaagttt ttaaacaatc
     4441 aaactaagaa cgagtaaaag ctcgtcggag aatttatgta actgaacacg gacggaaagc
     4501 tcagaattta gagaaaccaa cttggattgt caatccttcc ttgatttctt aaaattcaat
     4561 cgctttctat tcgtcctttg tatcttattt aatgtcgccc tgagaggcga cgaatagaaa
     4621 ggagaagata cattgttcgc ggaagtgaac acgccctaag aactgtgtga aaaagataaa
     4681 catcgtcttg acgctgaagg cgtctgcgcc gagtttccta ttttcactgt gttctttacg
     4741 ggctttgtat cataaactat gcctaaacgt actgatattc aaaaaattat ggtgattggt
     4801 tctggtccga ttattattgg tcaggctgct gagtttgact acgctgggac ccaggcttgc
     4861 ttgtcgttga aagaggaagg ttatgaggtt gttttggtta actcaaatcc tgcaaccatc
     4921 atgacggaca aggaaattgc tgacaaggtt tacattgaac cgattacact tgagtttgtg
     4981 acacgtattc ttcgtaagga acgtccagat gccttgctac caacactcgg tggtcagaca
     5041 ggtctcaaca tggccatgga attatctaaa aatggtatcc tagacgagct tggcgttgaa
     5101 cttctgggta ccaaattatc tgccattgac caagcggagg accgtgacct ctttaaacaa
     5161 ttgatggaag agcttgagca acccattcca gaatctgaaa ttgttaacac agtagaagaa
     5221 gcagttgcct ttgcagcgac aattggctac ccagtcatcg ttcgtccagc ctttaccctt
     5281 ggtggtactg gtggtggtat gtgtgccaac gaaaaagaat tgcgtgaaat cactgaaaat
     5341 ggtttgaaat tgtcacctgt tacccaatgt ttgattgagc gttcgattgc cggttttaag
     5401 gaaattgaat acgaagtgat gcgtgactca gctgacaatg ccttggttgt ttgtaacatg
     5461 gaaaactttg acccagttgg gattcacaca ggggattcca ttgtatttgc ccctgcgcaa
     5521 accatgtcag actatgaaaa ccaaatgctt cgtgatgcga gcttgagcat tattcgcgcc
     5581 ctcaagattg aaggtggatg taatgttcag ctagctcttg atcctaacag cttcaagtat
     5641 tatgttatcg aagtaaaccc tcgtgtatca cgttcgtcag cccttgcttc taaagcgaca
     5701 ggatatccga ttgctaagtt ggctgccaag attgcagtag gtttgacctt ggatgaggtt
     5761 ataaacccag ttacaggttc aacctatgcc atgtttgaac cagcccttga ctacgtagtt
     5821 gctaagattc cacgtttccc atttgacaag tttgaaaagg gtgaacgccg tctcggtaca
     5881 caaatgaagg cgactggaga agtcatggca atcggtcgta acatcgagga atctcttctc
     5941 aaggcctgcc gctcccttga aattggggtg caccacaatg aaatacctga acttgcagca
     6001 gtttctgatg atgccttgat tgaaaaggtt gtcaaagccc aagatgaccg tctcttctac
     6061 gtatcagaag ccattcgtcg tggctacaca cctgaagaaa ttgcagagct tacaaaaatt
     6121 gatatcttct atcttgataa actcttgcat atctttgaaa ttgagcaaga attgggtgct
     6181 catccacaag atctagaagt tttgaaaaca gccaaactta atggtttctc agaccgtaag
     6241 attgctgaac tctggggaac gactgatgat caagttcgcc aacttcgctt ggaaaacaag
     6301 attgttccag tttataagat ggtagatact tgtgcggcag agttcgactc tgaaacgcca
     6361 tatttctatt caacctatgg ttgggaaaat gaatctatca gatctgataa agaatctgta
     6421 cttgtcctag gttcaggtcc aatccgtatc ggtcaagggg ttgagtttga ctacgcaacc
     6481 gttcactctg ttaaggctat ccaggcagct ggttatgaag ctatcatcat gaactcaaac
     6541 ccagagaccg tttcaacaga cttctctgta tcagataagc tttactttga gccattgaca
     6601 ttcgaagatg ttatgaacgt cattgacttg gaacaaccaa agggagttat cgttcagttt
     6661 ggtggtcaaa cagccatcaa ccttgcggag ccattggcaa aagcaggtgt gaccatcctt
     6721 ggtacacagg ttgctgacct agaccgtgcc gaagaccgtg acctcttcga gcaagctctc
     6781 aaagaattgg atattccaca gccaccagga caaacggcta ccaatgaaga agaagcagct
     6841 cttgcagctc gtaagattgg cttcccagtt cttgtccgcc catcttatgt acttggtgga
     6901 cgtgccatgg aaatcgttga aaacgaagaa gaccttcgtt cttacatgcg aactgctgtt
     6961 aaggctagtc cagatcaccc agttcttgtc gactcttata tcgttgggca agagtgcgaa
     7021 gttgatgcca tttcagatgg taaagatgtg ctcattcctg gtatcatgga gcatatcgaa
     7081 cgtgctggtg tccactcagg tgactcaatg gctgtttacc caccacaaac cttgtcgcaa
     7141 aaggttcagg agaccatcgc agactacacc aaacgcttag caatcggtct tcactgcctt
     7201 ggaatgatga acatccagtt tgttatcaag gatgaaaaag tctacgttat tgaggtcaat
     7261 ccacgtgcca gccgtactgt tccattccta tctaaggtaa ccaatattcc tatggctcaa
     7321 gtagcgacca agctcattct tggtcaaagt ttatcagaac ttggctacca aaatggactt
     7381 taccctgaaa gcacccgtgt tcatatcaag gcacctgtct tctcctttac caaactagct
     7441 aaggtagaca gcttgctcgg tcctgaaatg aagtcaacag gtgaagttat gggttctgat
     7501 gctactttgg aaaaagctct ctataaagcc tttgaagctt cttatctaca cttgccaact
     7561 tttggtaatg tcgtctttac catcgctgat gatgccaaag aagaagcctt gaacttggct
     7621 cgtcgtttcc aaaacattgg ttatggaatc cttgcgacag aagggacagc agccttcttt
     7681 gctagtcatg gcttgcaagc ccaacctgtt ggtaagattg gtgatgacga taaggatatc
     7741 ccaagttttg tacgcaaagg aagaattcaa gctatcatta acacagtagg aacaaaacga
     7801 actgctgacg aagatggtga gcaaattcgt cgttcagcca ttgaacacgg ggtgcccctc
     7861 ttcacagcgc tagatacagc taatgccatg cttaaggtac ttgaaagtcg tagctttgtt
     7921 acagaagcga tttaaataga aaaataagat atagcagact gaa
//
LOCUS       DAVKFH010000083         7745 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKFH010000083 DAVKFH010000000
VERSION     DAVKFH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.083"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..372)
                     /locus_tag="VW006_001921"
     CDS             complement(<1..372)
                     /locus_tag="VW006_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9380716.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAAVFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPK"
     gene            complement(465..623)
                     /locus_tag="VW006_001922"
     CDS             complement(465..623)
                     /locus_tag="VW006_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9380717.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(694..1123)
                     /locus_tag="VW006_001923"
                     /pseudo
     CDS             complement(694..1123)
                     /locus_tag="VW006_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1218..1658)
                     /locus_tag="VW006_001924"
     CDS             complement(1218..1658)
                     /locus_tag="VW006_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9380718.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            1649..1957
                     /locus_tag="VW006_001925"
                     /pseudo
     CDS             1649..1957
                     /locus_tag="VW006_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2112..2435)
                     /gene="tnpB"
                     /locus_tag="VW006_001926"
     CDS             complement(2112..2435)
                     /gene="tnpB"
                     /locus_tag="VW006_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9380719.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(2416..2601)
                     /locus_tag="VW006_001927"
     CDS             complement(2416..2601)
                     /locus_tag="VW006_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380720.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2874..3485)
                     /locus_tag="VW006_001928"
     CDS             complement(2874..3485)
                     /locus_tag="VW006_001928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU9380721.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            complement(3508..4893)
                     /locus_tag="VW006_001929"
     CDS             complement(3508..4893)
                     /locus_tag="VW006_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU9380722.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            complement(4894..6669)
                     /locus_tag="VW006_001930"
     CDS             complement(4894..6669)
                     /locus_tag="VW006_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU9380723.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            complement(6732..7052)
                     /locus_tag="VW006_001931"
     CDS             complement(6732..7052)
                     /locus_tag="VW006_001931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU9380724.1"
                     /translation="MEANKYKIGIIGSHDIILPFSMIGFDIFSAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            complement(7042..>7745)
                     /locus_tag="VW006_001932"
     CDS             complement(7042..>7745)
                     /locus_tag="VW006_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU9380725.1"
                     /translation="SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEV
                     ESIWNEYETFNNIRVLDVGADLAYFKHLKLLSNELGEVLSQVIVAMIDFYNIITVKRG
                     LSQNKSHGDILQLLSDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQD
                     ATISSSELEFLCDLLLYKTLDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTI
                     PFESIKERIRPHYGS"
BASE COUNT         2443 a         1610 c         1222 g         2470 t
ORIGIN      
        1 tttagggcga ttgattttta cttccgtcgc atcaatcatt accgtgtcct cagaactgag
       61 aggagttctt gaaatcgtaa caccactttg aacaagagtt acttcaaccc attggctccg
      121 acggattaag ttgctttcgt gaataccaaa aacagccgca atttgttcat aagttcgata
      181 ttctcgcaca tattgaagag tggccataag aaggtcttct agacttaatt taggttttcg
      241 tccacctttt gcgtgtttaa gttgataagc tgtttttaat acagctaaca tctcttcaaa
      301 agtcgtgcgt tgaacaccaa caagacgctt aaatcgtgca tcggttagtt gtttacttgc
      361 ttcatcattc atagaactac tataccatat tttgtttcgc aggaagtcta ttgaaaaatc
      421 tccagactgg agaactcacg gatagttcct aatctggaga tttcttattt gcacttttct
      481 tgtacaactt tagtccatgg taaataagcc tctaaaacct ctttgtttac gagagtttcc
      541 tcgtttggaa gacattctag aagataggat agatatttct cgctatttaa ttgatgacgt
      601 ttagctgttt ccaacaaact cataataata gctcttgctt tagctccttc aaaactttga
      661 gaaaataacc accctcgctt ttttaaactg agcttaggct aaaagagtcc actggactct
      721 tttactccgt cccataacca atgatttaat ggcgcgttca gctagattat tggaaaggac
      781 cagatgtccg tctttcaaaa tggtcttaaa ggtttcttca tacttgaggc tgtattcaat
      841 tgcccttcct agttttgaac ctgctaaaac tgactgacgc cggcactaag caaagaagtc
      901 ttccattaag ggctggagat gttcttgacg tttctgtagt cgttcatcag ctggcaaagc
      961 ctcccagtct ctttccaagg aaaataactg atcacaataa gctaaacctt tagctcctaa
     1021 ggatgattta tccgcttgct tgggggggcg cttcaaaaaa cttccttctc acatgcgccc
     1081 aacatccaac aagttcagct tcttccagtt gacgataagc agacgagcgg ttcgaagttt
     1141 accaagctta gcgtcgcctt gctcctaaac cttggactgc tatcgcatag gcagaactag
     1201 aggactaaag tcctaagtta ctgccgcaaa atatcacaat gcacatagcc agaataatct
     1261 cctaggaatt cttgtactac tgaaccactt cgacactgat catggtggta aagcgtaatc
     1321 ccttgtttct ctgctttacc tgacaaaaaa gtccaatagt aagtcagctg actatcactc
     1381 tctagcaccc tataagaagt ttcatccgca tgaagtaagg gctgagtcaa tagtctctct
     1441 cgcaagaggt tataaagggg ctccaaatag tattgactcg ccttgatatg ccaattagag
     1501 atttccttac gtgtgattgg taaacccatc ctagcccaat cttcttcttg gcgataattg
     1561 ggtaccttca gattaaactt ctgatggatg gtgtgagcga taatagaagc tgagccaaag
     1621 ttatgcgcta aaggggcttt aggaatagga gatttcacaa gcttatccag atgattatct
     1681 tttactcgtt atggacaatg ctatatggca taaatcaagt accttaaaga ttccgactaa
     1741 tattggcttt gcatttattc ctccatacac accagagatg aaccccattg aacaagtgtg
     1801 gaaagagatt cgtaaacgtg gatttaagaa taaagccttt taaactttgg aagatgtcat
     1861 gaatcaactc caagatgtta tacaaggact ggagaaggag gtgataaagt ccatcgttaa
     1921 tcggagatgg actagaatgc tttttgaaag cagatgagta taaaaagaaa gtcctcattt
     1981 caatagaaat cacgactttc tgatggattt atagtaaaat gaaataagaa caggacaaat
     2041 cgatcaggac agtaaaatcg atttctaaca atgttttaga agcagaggtg tactattcta
     2101 gtttcaatct actatatttt tggagtgata gaaaagccct tcataagcca gtctacttgt
     2161 tctggtgtga gagctttgac atccttttct gtacttagcc aaatcaatct gccgttctca
     2221 aagcgtttat atagtagcca aaatccttga ccatcccagt aaaggacttt aaagcggtct
     2281 ttacgtccac cacaaaagag aaagacttga ccggagaaag gatccaattc aaagtgggtt
     2341 ttaaccagat aagccagtga atcgattcct tgtctcatat cagttttccc acacacgaga
     2401 tagacctccc ctaggctaga tagatgaatt gtcatagagc aacaccttat ccaaaagctg
     2461 ttctaccatt tcgagattga gagattgaaa aaaacttact tcaagcttgc caatacgaat
     2521 tttaagcaga atatcatttc tctttttaga atcaaaacga cgagattgtg gaatctctac
     2581 aggaacaata ggttgtggca ttaaaatatc ctccaatagt tttactttac taatagtata
     2641 caggaggagc tccatgaatg atagatacct tgttatgacg cgcttacttt ctatataatt
     2701 acatttacga tttactaatc ctaaatctag attagtagaa acattttcat tatctaacaa
     2761 accaaaattt tgaaaaagat atcctgatac tgtcaataaa aaaagcttag aaaggcttaa
     2821 agccttatcc aagctgatat ttgttcatct aattaataat ctaaataaag gatctacttc
     2881 actttcataa tacgaactaa attggctctt tctgcctcct ctagtttcaa ttctatataa
     2941 tgaatagttt ccgacaagtt tggaataatc gagtattcta aaccatttac acgtctacgt
     3001 gttttttcta tttcatcagc cattaactga cacgtttttt caacttctgc cagtcttagt
     3061 aatttataaa ttaaactatt cattgtagca aatacatcat ccatttcact attagaagat
     3121 aaatagctgt attcactgtt ctcattttga gaagtaatat tcatatgcat tctaggaact
     3181 gttacactca tgatattttc tttctcaaca aataattcaa tttctttcga tggaattgaa
     3241 aataattcct ccaccatttg agaattcttt aatgatttag caactgcaaa ggattttaga
     3301 ttatcaatta gataactttc cacttctttc cgaagttgat tattctcacg aatcaaagaa
     3361 ataaatcgcc tcatcaattc atctctttta tcctttaata acttatgtcc acgttcagct
     3421 gttgtcaaac gttcctttaa gttattcaat tccatacgag ttggttttac attcaaacgt
     3481 accatagata atcactccat ttccggatta aacttctacc aaaggtaagt atttatcaag
     3541 caaatcatct ttgatacgtt ttaactctgt tctaggaaga attgatagta attcccaccc
     3601 aagattcaac gtatcttcta tatttcgatt tttataaaat ccttggttta tgtactcttc
     3661 ttcaaaacgc tttgtaaacc tcacatacaa tttatctaca tcagataaag ccgattctcc
     3721 taatactact gctaactctt caaccttttt cccttgggca taggctgcaa acagttgatt
     3781 catagttgga gcatgatctc cacgagtttt accttctcca gatcccttat cttttaatcg
     3841 agagagagaa ggtaaaacat tgattggtgg acgataacct tgattataca actcatgcga
     3901 caaaataatt tgcccttcag taatgtatcc agttaaatca ggaattggat gtgttatgtc
     3961 atcttctggc attgttaaaa taggaatctg tgtcaccgaa ccttttttac caactaagcg
     4021 accagccctt tcgtatagag ttgataaatt tgtatataaa tatcccggat agcctcgtct
     4081 ccctggaact tcacggcgag ctgccgagac ttcacgtaac gcttcacaat agttagtcat
     4141 atccgtcatg ataactagaa cgtgcatatc tttttcaaaa gctagatact ctgccgcagt
     4201 taaagcaatg cggggagttg caatacgctc aattgcagga tcatttgcca agttcataaa
     4261 taaaaccgaa cgatcgatcg ctcctgtttt tctgagttct tccataaaaa actcagcttc
     4321 ttcaaaagta atacccattg ctgcaaatac aaccgcaaaa ttttcatcag aatttaaaac
     4381 agtcgcttgt cttgctatct gagcagctaa ttcattatga ggtaagcccg aacctgaaaa
     4441 tactggtaat ttttgaccac gtacaagagt attcaaatga tcaatagagg agatccctgt
     4501 ctgaataaat tcatctggat aatctctaga tacaggatta atagcttgac catcaatatc
     4561 taaatatttc tctggaatta aatctggtcc accatcaatt ggttttccca tcccattaaa
     4621 aatacgacca accatatcct cagatacagc caattctaat gcatgaccag caaaacgaat
     4681 tttagacttt tctaaattta ttccactaga tccttcaaaa agctgaacca ttgctttatc
     4741 ttcgtggatc tctaaaactt gtccacgacg aatttctcca ttatgaagtt gaatttcaac
     4801 taactcattg taagacacat tatttacttg ttcaacaatc ataagaggcc caacaacttc
     4861 actagcagtt ctgtattctt ttataacact catttataga cctcctgttt ctaatatcaa
     4921 atgaatttga tgtgtaatct catttgatat aattttgatt tcatctaatc tatcttctga
     4981 aacatattta cttctcgcca tacggtctcg aactgccacg gtaccttcca taatctctgt
     5041 aaagtaagaa cccaactcta aagcatgatt tgcctgatca gcaaaagtga gaatattact
     5101 tagcattgct tcttgttttg caaacgaagt gaatgtatct accgaatcaa aagcgttctg
     5161 ttgcaaataa tcttctcgaa tttgtttagc aatttccatc gttagtcgtt cattatcaga
     5221 cagagaatca attccaacaa gacgaacaat ttcctctaaa ctagattccc gttgtaagta
     5281 gttcatcgca cgagttattt tactattcca atctgtcttc tctttaccat ctatataagt
     5341 gcccacactg tctttatata gtgaataaga tgtaagccag ttaattgcag gaaaatgacg
     5401 tcgctgtgcc aacggagcat caagccccca aaaaactttc acaatccgta aagtgttttg
     5461 agtaactggt tctgaaatat ctccaccagg tggcgataca gctccaatag cagtaatcgt
     5521 tccttcacgt tctggaagcc ctagaacctg agaacgtcct gctctttcat aatattcagc
     5581 gatacgactt cccagataag caggataacc ctcatcacca ggcatttctt ctagacgtcc
     5641 tgacatttca cgtagcgctt ctgcccaacg tgaagttgaa tcagccataa tggcgacaga
     5701 gtagcccata tcacgaaaat actcagccat ggtaattcct gtataaattg aagcctcacg
     5761 agcagcaaca ggcatatttg aagtattagc aatcagaact gtccgttgca taattgattg
     5821 tccggtatta gggtcaatca actcaggaaa ctcattcagt acatccgtca tttcatttcc
     5881 acgttctcca caaccgacat aaataacaat atcaacattg gcaaatttag ctacttggtg
     5941 ttgtacaact gtctttcctg ctccaaacgg tccaggaact gctgcagctc cccctttggt
     6001 tactggaaag aatgcatcaa taactcgttg acctgtgatt aatggttctt ctggaattaa
     6061 acgtttagaa acaggacgcg ccttgcggac aggccatttt tgcataagcg ttcctttata
     6121 gaaactaccg tccaattttt ttatttcata tacaacttca tcaattgtaa aatcgccaga
     6181 tgcaatagaa acgacttctc cagatactcc ataaggaacc ataattttat gattaactac
     6241 ctcggtttcc ttgacagttc caagaatatc acccgtactc actttttgac caattgctat
     6301 agtggaatca aaatgccact taatatctct atccaaactt ggaacttcta ccccacgaac
     6361 tagaaaatca ttatgagtag ccaatttaaa tcgatctaat gggcgttgta tgccatcaaa
     6421 catttgagaa atcaatcctg gccctaattc aaccgagaga ggttctccag ttgtaacaac
     6481 aggttctccc ggaccaagac cagatgtttc ttcatagact tggatagatg cctgatctct
     6541 tctcatttca ataatttcac cgattaaccc tagcttacct acacggcaaa tatcttgaat
     6601 attagcctcc tgcatacctg atgcaataac tagaggtccc gatactttta taatcttccc
     6661 ttgagtcaaa gttattctcc ttactaagtc caaaaattat agaataatat tacagacaat
     6721 tttgtacatt attataaaat attgtgtcct actgctttct ctacattatc ctctatacgt
     6781 tttaatccta aatttaaacc ttgtttatga gtcggtaata aaataatagc aggcacaact
     6841 tgagaatcat aatggcgaat tgtatctaat atcattgaag caatgtcttc agtgatataa
     6901 atgacaccat aatcagattg agctaatttt cttagagtat ttatagcttc ttgttcttga
     6961 taggcagaaa atatatcaaa cccaatcatg ctaaagggta aaataatatc atggctacca
     7021 attatgccaa ttttatactt attagcttcc ataatgcggg cgtatccttt cttttattga
     7081 ttcaaaagga attgtatttt gaagagctga tatgatcatt ctaagattct ttacttcaaa
     7141 ctcacatccc aataaatatc tagcaagaac taacggccca tctacattat accttccttg
     7201 atctaaagtt ttatacaata gtaaatcaca taaaaattct aactcagaag atgaaattgt
     7261 tgcgtcctgc atcttcaatt cgtaagttga aaagattgaa tctaagcttg gatttatttt
     7321 attgaaccac acaaatattt cttgattttc tacaatgtat ataaattctt tagcagaaat
     7381 acttccttca tctgaaagta attgtaaaat atccccatga ctcttatttt gagataaacc
     7441 acgttttaca gtaataatat tataaaagtc aatcattgca acaataacct gagacagcac
     7501 ctcacctaac tcattagata aaagtttcag atgtttaaaa tatgctagat cagctccgac
     7561 atcaagtaca cgaatattat taaaagtttc atactcattc caaattgatt ctacttcatg
     7621 aaccataaaa tcaggaagtg tatctgaatg taaggaagaa actaaatgtt ttaaactttc
     7681 tatatcaaaa atccctattg gaattaataa tttagaaaaa tctttcttaa ttgccgcctt
     7741 agatt
//
LOCUS       DAVKFH010000084         7292 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKFH010000084 DAVKFH010000000
VERSION     DAVKFH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.084"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..986)
                     /locus_tag="VW006_001933"
     CDS             complement(<1..986)
                     /locus_tag="VW006_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380726.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDY"
     gene            1146..2900
                     /locus_tag="VW006_001934"
     CDS             1146..2900
                     /locus_tag="VW006_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380727.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            2884..4605
                     /locus_tag="VW006_001935"
     CDS             2884..4605
                     /locus_tag="VW006_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380728.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            4616..5200
                     /locus_tag="VW006_001936"
     CDS             4616..5200
                     /locus_tag="VW006_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9380729.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            5200..5895
                     /locus_tag="VW006_001937"
     CDS             5200..5895
                     /locus_tag="VW006_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9380730.1"
                     /translation="MSTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            5883..7247
                     /locus_tag="VW006_001938"
     CDS             5883..7247
                     /locus_tag="VW006_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380731.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSINVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFIFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         2617 a          969 c         1294 g         2412 t
ORIGIN      
        1 caataatcgc atttaaaatt gtgatgtttg caagctaaat tacggacttc acttggaagt
       61 tttcccttgt atcttttata atagatagaa aatttgctgg cagatgaata tccaacagat
      121 tctgctatct cttttatagg tagttcagtg tttaaaagaa gagtttcagc tacattcatt
      181 ctttttcttt gagtgtactc tgtaatgctt tgacaatatt tttccttaaa taaatttttt
      241 aatttagtac cactcatttt agatattttt tcaagcgtgc cttgatttac attcgttgca
      301 aaatgatcat ctaagaatct tgctacatct tcaagtgctt tatcatcatc aatttcaatt
      361 ttatattttt ttctatttaa gtatgtgtca attactatac ttatccattc atttgccttt
      421 gctttaaaga aaaaatcagc ggcaggagcg tccatcttac aatttaatat ttccattgcc
      481 actctttcta aggcctttgt aagtattatt tgattcggtt gaagcaaggt tgaataaaaa
      541 gattctggat taatgttaat agatgctaaa tgtttttcta ttagctcttt tttaaaacca
      601 atggaaacag caagataaca acaattctcg tgtaataaaa aaacaaaatc atcttttata
      661 ttatcaaaat caaaagtaca taaagagttt gcggtaatag tttgatacgg attaaacttt
      721 tctccgtttg cactgacaat gtaacttgaa taaattgaaa catagtctga catactataa
      781 gtgctatttt gaactacttc ctctttgata taaaaatcat gtatatcgat aatgaagctg
      841 cctccttcat aaaaccaata taggccttct gcataagttg aattctcttt tttccaacaa
      901 aatgtatgac ctgcacttga atacttctca ttgtttttac attcatctac tttcatataa
      961 tcttttacaa aatcataata tgacataaca cactatccct tttagacaat attccaatta
     1021 gccttattaa ttcaaaacta ttgtattagt aattataaca gatgtataat agaaaagcaa
     1081 tgatagatat tatcaattaa gcgaatttat atctaaaagg gatattaaag aaaggagata
     1141 tgcttatgaa gatttacaaa aaactatttg cttatgtcca agataagaaa tatcttgggg
     1201 ttttggccat aattttttct gctatatctg ctctacttac agtatatgga tattatttaa
     1261 tctacaaatt tctagataag ttaataatta attcaaactt atccggtgca gagagtatag
     1321 cattaaaatc tgttattaca ctaacaagtg gagcgatatt ttattttgtc tcaggaatgt
     1381 tttcacatat cttgggattt aggcttgaaa caaatttaag aaaaagggga atcgatggtc
     1441 tggaaaaagc aagttttagg ttctttgact taaatccatc tggtcaaata agaaagatca
     1501 tagatgataa tgcagcacaa actcatcagg tggtagcaca catgattccc gatagttctc
     1561 aggcaataat cacacccgta cttgtacttg cacttggctt tatagtaagt ataagggttg
     1621 gcataatttt gcttgctctt actataattg gtggcttaat tttaggggca atgatgggcg
     1681 agcaagaatt tatgaagata taccaagaat ccctatctaa actaagtgct gaaactgttg
     1741 agtacgtgag aggaatgcaa gttgtaaaaa tatttaaagc aaatgtagag tcttttaaaa
     1801 gcttttataa ggcgataaaa gattactcaa agtatgctta tgattattcc ctatcttgta
     1861 aaaggcctta tgttttgtat caatggttat tttttggact gattgcaatt ttaattattc
     1921 ctatagttta ttttatgact agcttagcta gcgcaaaggt gattttactt gagcttatca
     1981 tgattttatt tttatcagga gttctctttg tttcattcat gagaatgatg tggtactcca
     2041 tgtatatttc tcaaggaaat tatgcagtag atactttaga ggcgctttac gaagatatgc
     2101 aaaaagacaa attagtgcat ggtaatgtca ataattttaa aaactataat atagaatttg
     2161 agaatgttag ctttgcttat aatgataaag ctgtcattga aaatttatcc tttaatttag
     2221 aagaaggaaa gtcctacgca cttgtcggtt catctggatc aggcaaatca acagtagcaa
     2281 aacttatatc aggtttttac aatgttaata aaggaagcat aaagataggc gggatagcaa
     2341 taagtgaata ttctgacgaa gccttaatta aagccatttc ctttgttttt caagattcaa
     2401 aattattcaa gaagagcatt tatgataatg tagctttagc taataaagat gcgacgaaag
     2461 atgacgttat gagagcctta aaattagcag gatgcgattt aatattagac aaattcccag
     2521 aaagagaaaa tacaatcata ggctcaaaag gtgtttattt atccggtgga gaaaaacaaa
     2581 gaattgcaat tgctagagca attttaaagg attccaaaat tattattatg gatgaagcat
     2641 cagcatctat tgacccagat aacgagtttg aattgcaaaa agcttttaaa aatcttatga
     2701 aggataaaac agttatcatg attgcacaca ggctatctac aattaaagac cttgatgaaa
     2761 ttattgtcat ggatagtgga aaaattatag aaagagggtc tgacaaagaa ttaatgtcaa
     2821 aagatacaag gtataagagc ctgcaagaga tgtttaacag tgcgaatgaa tggagggttt
     2881 caaatgaaag agttttataa aaaaagattt gctcttacag atggaggagc aagaaattta
     2941 agtaaagcaa cactggcttc atttttcgtt tattgtataa acatgcttcc tgccatatta
     3001 cttatgattt ttgctcagga agttttggaa aatatgggca aaagcaatgg cttttatata
     3061 gtattctcag ttttgacttt gatagcaatg tatattttgc tttctatcga atacgataaa
     3121 ttatataaca caacctatca agaaagtgca gatttaagaa taaggacagc ggagaattta
     3181 tcaaaattac ctctatctta cttttctaaa catgacattt ccgacatttc acaaacaatc
     3241 atggctgata ttgaaggcat agagcatgca atgagccact caataccaaa ggtgggcggc
     3301 atggtactgt ttttcccatt aatatctgta atgatgctag cgggcaatgt caagatgggt
     3361 ttagctgtaa ttattccatc tattttaagc tttatattta tacctttatc taaaaaatat
     3421 caggttaatg gacagaatag atattatgat gtcttaagaa aaaactcaga aagctttcaa
     3481 gaaaatatcg aaatgcaaat ggagattaaa gcatataatt tatcgaagga tattaaagat
     3541 gacttatata aaaaaatgga agatagtgag agagtacact taaaggcaga agtaactaca
     3601 attttaactt tgtctatatc ttcaatattt agctttatat ctcttgctgt tgtgatattt
     3661 gtcggcgtaa atctaattat taataaagag ataaattctc tctaccttat aggatattta
     3721 ctagctgcta tgaagataaa agactcttta gatgcatcta aagagggctt gatggaaata
     3781 ttttatttat cgcccaaaat agaaagatta aaagaaattc aaaatcaaga tttacaagaa
     3841 ggcgatgact atagcttaaa aaaatttgat attgatctaa aagatgttga gtttgcctac
     3901 aataaagacg caaaagtttt aaatggtgta agttttaaag ctaagcaggg agaggtcact
     3961 gctttggtag gtgcaagtgg ctgcggtaaa acaactatct tgaaacttat atcaagactt
     4021 tatgattatg acaagggaca aatcttaatc gatggcaaag atataaaaga aatatcaaca
     4081 gaatctcttt ttgataaggt ctctatagtt ttccaagatg tggttctctt taatcaaagc
     4141 attatggaaa atattagaat cggaaagcaa gatgcaagtg acgaagaggt taaaagagca
     4201 gcaaaacttg caaattgcac agattttata gaaaaaatgg ataaaggttt cgatacagtt
     4261 attggtgaga acggagctga gctatcagga ggagaaagac aaagattatc aatagccaga
     4321 gccttcttaa aagatgcgcc gatattgatc ttagatgaga taacagcaag ccttgatgtt
     4381 aacaacgaga aaaagattca agagtcttta aataatttag ttaaagataa aactgttgta
     4441 atcatttcac atagaatgaa atccatagaa aatgcagaca agatagtagt tcttcaaaac
     4501 ggaagagtag aaagcgaagg taagcatgaa gagcttttac aaaaatcaaa aatttacaaa
     4561 aatttaatag aaaagacaaa aatggcagaa gaatttattt attaggagga ctacaatgga
     4621 taataaaaaa ttaaaagtaa aagatttagt aagtatcggt gtttttggcg taatttattt
     4681 tgccttcatg tttggagttg gtatgatggg cttgattcca atattgttct taatataccc
     4741 gacagtatta gccatagttg caggaactgt tgttatgtta tttatggcta aggttcaaaa
     4801 gccatgggca ctatttatat ttggtatgat atcaccactt gtgatgtttg cagctggtca
     4861 tacctacgta gttgtggttt tatcacttat agtaatgata atagcagaat taattagaaa
     4921 gattggtaat tataattcat ttaaatacaa tatgctttct tatgcaatct tcagcacatg
     4981 gatatgtagc tctttaatgc aaatgctttt agcaaaagaa aaatatatgg agtggtcttt
     5041 gatgacgatg ggaaaagatt atgttgatgt attagaaaag ttaataactt atcctcacat
     5101 ggctttagta gccttaggtg ctttcttagg aggaattctt ggagcatata taggcaaggc
     5161 tctattgaaa aaacactttg aaaaagcagg cattgtataa tgtctacttt ttcttcaaat
     5221 gctcccttta atcccaatcc gatctctaag atattagtcg tatttatcac aggcttaact
     5281 gttatgcata gcataaacat ccgttttgag ctggcaattg tttgtcttat tggtattttg
     5341 ctttatttga atggatacaa aaaaacactt ttcaaatgga ttatattgtg tgggatactt
     5401 tactccttgc ctaattttat ggtgttatct gaattaaacc ctatagttaa gatgtttttg
     5461 agtataccta ttgttattag aatgtttatt ttaccattta tggcagcaag ctttatgata
     5521 aagacctcgg atgtaggcgc aataatttca tcgatggata agcttaagat ttcaaagaat
     5581 gtatccatac ctattgcggt tatgtttaga ttcttcccat cttttaagga ggagaagaaa
     5641 aacatcaaaa tggctatgag agtaagaggg ataagtttta aaaacccagt caaatatctt
     5701 gaatatgttt ctatgccact actcattata tcatctaata tatcagatga cattgcaaaa
     5761 gcggcagaaa caaaggcaat agaaaatcca attgccaaga ccagatacat tcgcgtaaag
     5821 atacagctaa ttgattttgt ttatgtttta gcggttgctg gacttattgt gggaggctta
     5881 atatggttga aataaaaaat ttaagtcttg attatggtga agagcatata ttagatgata
     5941 tatcactatc catagccgag ggagagtgcg tgctatttac aggaaaaagt ggaaatggta
     6001 agtcatcttt aataaattca atcaatggac tagctgtaag gtatgataac gcaaagacaa
     6061 agggcgaaat aattattgat ggtaagaata taaaaaattt ggaactttat caaatctcaa
     6121 tgcttgtttc aactgttttt caaaatccta agacatattt ttttaatgtc aatacgacat
     6181 tagaattatt attttatttg gaaaatatcg gtcttgcaag agaagagatg gacaggcgtt
     6241 tgaaggatat gcttaagata ttcccgataa aaaatctttt gaacagaaat atatttaatc
     6301 tatccggcgg tgaaaaacaa attctttgca ttgcagcttc ttatatagca ggtacaaaga
     6361 ttatagttat ggatgagcct tcatcgaatt tagatattaa aagcataaat gttttggcaa
     6421 agatgctaaa gatattaaaa gagaaaggca taagcataat tgttgcagag catagaattt
     6481 attatttgat ggacatagtt gaccgtgtat ttttaataga taaaggaaag cttaaaaaaa
     6541 cttatactag aagtgaattt ttaaagctag ataaaaatga attaaatgct ttaagtttaa
     6601 gagataaaga attaagtaaa ttaaaagttc cttatttaaa agaaggtgga gagtatcaga
     6661 taaaaaatct tagctacaaa tttactgatg atgagtgttt aagcttaaaa gatatttcat
     6721 tcaagcttgg gaaaatttat ggcataatag gatccaacgg acgaggaaaa tcaacgcttt
     6781 taagatgttt aataggtctt gagaaaaaat caaaagaaga aatttatttt aagggagaga
     6841 agctatctaa aaaagaaaga ctcaaaaact cttcacttgt tatgcaagat gtaaatcatc
     6901 aattattcac agatgaagta ttcaacgagc ttagattagg agtaaagaat tttgatgaag
     6961 aaaaggcgaa aatcatttta aaagatttag gcctggacga atttattgaa aggcatccga
     7021 tgagtttatc aggagggcaa aagcaaaggc ttgcaatagc atctgttatg tgcaagaatt
     7081 ctccatttat cttttttgac gaaccttcaa gtggtatgga ttattccaat atgataaaaa
     7141 tatctgaact gattaataag tataaaacca tggataaaat aatttttatt gtttcccatg
     7201 atatagaatt tttaaatgaa gtggcagatg aaatttttga attgtaaaat agagtctgtt
     7261 aaacatgtct atagaactag taaattgtct aa
//
LOCUS       DAVKFH010000085         7088 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKFH010000085 DAVKFH010000000
VERSION     DAVKFH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.085"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            226..6660
                     /locus_tag="VW006_001939"
     CDS             226..6660
                     /locus_tag="VW006_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9380732.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         2782 a          966 c         1367 g         1973 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcaaaggata ctgaagaaaa gaaaacaaca gttattgagg aaaaagaagt tgttagtaaa
      481 aatcctgtga tagacactaa aactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagataatgc acactcgtct gcaaataaag gcacagaaaa tccgaaaaaa
      601 gaagatagac ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 acatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgca gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctattcgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcag gctgatgaaa aggggatgat ttcctattat
     4201 ctagtagatc ctgatcaaga ttcatctaaa tatcaaaagc ttggcgagat tgcagaatct
     4261 aaatttaaaa atttaggaaa tggaaaagag ggtagtctaa aaaaagatac aactggggta
     4321 gaacatcatc atcaagaaaa tgaagagtct attaaagaaa aatctagttt tactattgat
     4381 agaaatattt caacaattag agactttgaa aataaagact taaagaaact cattaaaaag
     4441 aaatttagag aagttgatga ttttacaagt gaaactggta agagaataga ggaatacgat
     4501 tataaatacg atgataaggg aaatattatt gcttatgacg atggtagtgc cttacaatat
     4561 gaaactgaaa aacttgacga aataaaatca aaaatttatg gcgttctaag cccatctaaa
     4621 gatggacact ttgaaattct tggaaagata agcaatgttt ctaaaaatgc caaggtatat
     4681 tatggaaata gctataaatc gatagaaatc aaagcgacta agtatgactc acattcaaaa
     4741 acgatgacat ttgatttata cgctaatatt aatgatattg tggatggatt agcttttgct
     4801 ggagatatgc ggttttttgt taaagatgat aatcgaataa aagctgaaac taaaattaga
     4861 atgcctgaaa aaaataagga aactaaagca gaatatccct atgtatcaag ttatgggaat
     4921 gtaatcgaat taggagaagg agatctttca aagaacaaac cagacaattt aactaaaatg
     4981 gaatctggta aaatctattc tgattcagaa aaacaacaat atctgttaaa ggataacatc
     5041 attctaagaa aaggctatgc actaaaagtg actacctata atcctggaaa aacggatatg
     5101 ttagaaggaa atggagtcta tagcaaggaa gatatagcaa aaatacaaaa ggccaatcct
     5161 aatctaagag tcctttcaga aacaacaatt tatgctgata gtagaaatgt tgaagatgga
     5221 agaagtaccc aagctgtatt aatgtcggct ttggacggct ttaacattat aaggtatcaa
     5281 gtgtttacat ttaaaatgaa cgataaaggg gaagctatcg ataaagacgg aaatcttgtg
     5341 acagattctt ctaaacttgt attatttggt aaggatgata aagaatacac tggagaggat
     5401 aagtccaatg tagaagctat aaaagaagat ggctccatgt tatttattga taccaaacca
     5461 gtaaaccttt caatggataa gaactacttt aatccatcta aatctaataa aatttatgta
     5521 cgaaatccag aattttattt aagaggtaag atttctgata agggtggttt taactgggaa
     5581 ttgagagtta atgaatcggt tgtagataat tatttaatct acggagattt acacattgat
     5641 aacactagag attttaatat taagctgaat gttaaagacg gtgacatcat ggactgggga
     5701 atgaaagact ataaagcaaa cggatttcca gataaggtaa cagatatgga tggaaatgtt
     5761 tatcttcaaa ctggctatag cgatttgaat gctaaagcag ttggagtcca ctatcagttt
     5821 ttatatgata atgttaaacc cgaagtaaac attgatccta agggaaatac tagtatcgaa
     5881 tatgctgatg gaaaatctgt agtctttaac atcaatgata aaagaaataa tggattcgat
     5941 ggtgagattc aagaacaaca tatttatgta aatggaaaag aatatacatc atttgatgat
     6001 attaaacaaa taacagacaa gacactaaac attaagattg ttgtaaaaga ttttgcaaga
     6061 aatacaaccg taaaagaatt cattttaaat aaagatacgg gagaggtaag tgaattaaaa
     6121 cctcataggg taactgtgac cattcaaaat ggaaaagaaa tgagttcaac gatagtgtcg
     6181 gaagaagatt ttattttacc tgtttataag ggtgaattag aaaaaggata ccaatttgat
     6241 ggttgggaaa tttctggttt cgaaggtaaa aaagacgctg gctatgttat taatctatca
     6301 aaagatacct ttataaaacc tgtattcaag aaaatagagg agaaaaagga ggaagaaaat
     6361 aaacctactt ttgatgtatc gaaaaagaaa gataacccac aagtaaacca tagtcaatta
     6421 aatgaaagtc acagaaaaga ggatttacaa agagaagatc attcacaaaa atctgattca
     6481 actaaggatg ttacagctac agttcttgat aaaaacaata tcagtagtaa atcaactact
     6541 aacaatccta ataagttgcc aaaaactgga acagcaagcg gagcccagac actattagct
     6601 gccggaataa tgtttatagt aggaattttt cttggattga agaaaaaaaa tcaagattaa
     6661 gacaaaagct atagaaaaaa atggtttatg tactgagatt agatagtgag gtgatgacat
     6721 agttttgtga aaacggccat ttataactca attatttagt ttacttcact tcactattga
     6781 tactatttgg agttattaac ggacttagtt tagataacta atgaattgat tgaaagggtt
     6841 agtattgaca atattggtca tattgattag aaaatagagt ctatcaaaat ttaaaggcta
     6901 atagaggtga tgagacaatt tcaaggaatt aagtagcgtt tggttgctta attccttttt
     6961 atttggaagg aagtttttcg taaccgccca ataacgaagt ctattggacg gttacgatat
     7021 gatcactgac ttcgtcagtc ttatctacaa cctcaaaact gtgttttgag caacctgcgg
     7081 ctagcttc
//
LOCUS       DAVKFH010000086         6953 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKFH010000086 DAVKFH010000000
VERSION     DAVKFH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.086"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(102..377)
                     /locus_tag="VW006_001940"
     CDS             complement(102..377)
                     /locus_tag="VW006_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9380733.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(484..1323)
                     /locus_tag="VW006_001941"
     CDS             complement(484..1323)
                     /locus_tag="VW006_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9380734.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1465..2313
                     /locus_tag="VW006_001942"
     CDS             1465..2313
                     /locus_tag="VW006_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9380735.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2406..3323)
                     /locus_tag="VW006_001943"
     CDS             complement(2406..3323)
                     /locus_tag="VW006_001943"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9380736.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3760..3965
                     /locus_tag="VW006_001944"
                     /pseudo
     CDS             3760..3965
                     /locus_tag="VW006_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VW006_001945"
     CDS             complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VW006_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9380737.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4586..4930)
                     /locus_tag="VW006_001946"
     CDS             complement(4586..4930)
                     /locus_tag="VW006_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9380738.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VW006_001947"
     CDS             complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VW006_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9380739.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5296..5383)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5442..6146)
                     /locus_tag="VW006_001948"
     CDS             complement(5442..6146)
                     /locus_tag="VW006_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9380740.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6548..6847
                     /locus_tag="VW006_001949"
     CDS             6548..6847
                     /locus_tag="VW006_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9380741.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2189 a         1411 c         1159 g         2194 t
ORIGIN      
        1 gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa gcctactgac
       61 caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac agcgtcttta
      121 agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat ttctttacca
      181 gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt accaaaaccg
      241 atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa tacagcttca
      301 actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt agcgatcaaa
      361 tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac aaatataatc
      421 atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat tttatttatt
      481 tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt gagtttgaat
      541 aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta agctttcttt
      601 catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg ctacagcacg
      661 ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag ttccacgacc
      721 tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga tattgagaag
      781 agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat tttccaaagt
      841 tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag ataaaattgc
      901 ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga atttcagggc
      961 ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc tagcaccttg
     1021 gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa gaatctggcc
     1081 accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg gctgacttgt
     1141 tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt ctttcaaatc
     1201 cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg taatatctaa
     1261 ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct taatttttgt
     1321 catagtatca atctttctat tttaggattc agattggttt ccttacttct aattatatca
     1381 aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt tgatataatc
     1441 aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt taccatttta
     1501 ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca tcatctcttt
     1561 gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat ccctgtttcc
     1621 ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat ctttggagcc
     1681 aaatccctct attctagttt actaggaacc ttagctttgt ccgcctggtt agcttttttt
     1741 gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc ccttatagcg
     1801 ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac aactggcgga
     1861 actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg aaaactgctc
     1921 tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga tttgagattg
     1981 gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt gattggtgaa
     2041 ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca acttgctaag
     2101 gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg ctactatagt
     2161 aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt gaaaacgaag
     2221 gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc ccatgaaatc
     2281 ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg acctcaaaag
     2341 ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt tatttactca
     2401 agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc cactaattgg
     2461 tcaactgagt caaatttggt catatcacga atgcgatcaa gccaataaac catgacggtt
     2521 tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc ttcttctcca
     2581 tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct ttgaatctca
     2641 acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag cactaaattc
     2701 gctgtcggat aaccaattgt acgaccacga gcattaccat gaaccaccat acctcttgat
     2761 ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa aatagcttga
     2821 cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac aatgataact
     2881 tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc aaatgtataa
     2941 tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc aaagaattct
     3001 tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc ttctacacct
     3061 tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa caaatctgga
     3121 tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac gataggcaat
     3181 aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc cttatgtatg
     3241 ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc tttttggttt
     3301 tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta tttctggaac
     3361 agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa aagcacttta
     3421 ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg taggacatct
     3481 tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc atactatgtt
     3541 atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat tggtgccaaa
     3601 aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa tgagacaact
     3661 tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc actttccctg
     3721 atgacaaaga aaaaagccaa aacagcaaag aaaaactaga tgactgcttt ccaacaactc
     3781 ccatctagtg tgcttcagac tgggctattt ttctctccat ctgttagctt ggattctcag
     3841 accgtttcag ctaaagaata tcttttccct tatcagaagg aacggctcaa accattcaga
     3901 caagtgaagg gacgacaagc caatatttga aaccagatag cagttcttat agtcaattga
     3961 aataaaatct gaagaaatcg agtaggaaac tcatatcaat ttttaacagt gttctattcc
     4021 agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag gctgtacttg
     4081 agtacggcaa ggtgaaattg acgtggtttg aatttgattt tcgaagagta tcaatccact
     4141 atacatatta aaaagtatgg gtatcaaaat aaaaaaacag gctctccgaa aactcggaaa
     4201 gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc gggataaaag
     4261 gttcaatgaa cctttttatt ttgcgattgg gtaaacagaa acttgttttt tatcgcgtcc
     4321 tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt ctccaccacg
     4381 accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga ttgatccacc
     4441 tgttacagtt tgtccgtcag ctgctttagc tccaagacgt tttgcttgtg aatcacgtcc
     4501 gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt taagagtcat
     4561 ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt tggacgaact
     4621 cagaatagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag aataactggg
     4681 tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga tagccacctt
     4741 catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata gaattgataa
     4801 agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat tcgccactct
     4861 cggcgtgtcc agtaatttcc gcactcctca gctcgccatc ttcggctctc tcaaagactg
     4921 cctgtatcat gtgttctcct taaaattaag cgttgattgc gttgatgaca acttttgtat
     4981 atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac ttgtaagtaa
     5041 ccactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct ccagcaacaa
     5101 gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta aaagtaactt
     5161 cttgaccagc ttcaacgttc aatttttcaa cgtaaactgc ttgaccaact tcaactttaa
     5221 cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc ctatgatttt
     5281 tatgggtttc cccgattttt tgtgaagact cgcctagcat cgtgggacga accacttaaa
     5341 cttgaataat caagcaacga tgttcgtgcg gttgcacagg atcgtgcata gtcaactctt
     5401 caagtatagc atatctccta ttttcttaca agtaataaca cctaaaatga agctttttct
     5461 tttacttttt tctgccaaga ggcaaaaagc atgctgaggt aaaaaacgct catcataata
     5521 ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca aataggctga aaagacaacg
     5581 ccaaggacaa aactactaag caggctaaca aatatgaatc cttcacgcaa aaaaggagtg
     5641 tgcttggttc ggaaataatc tccaaaagcc agcatggtcc gtttgatatt ccctgtcata
     5701 aaagcgttat tataggcaat acccgacact tctccaaaag cagttgtcac cagtcccata
     5761 cagaaggcca agggcggcac tagatagata ttatccacag tttgcggcac aaaagcaata
     5821 atgattgata agattgccaa gggaatcaag gacagaatag gttttttcac aattctcaat
     5881 ttttccttat aaatcgttaa taaaaagact cccatcataa acgctagcaa ggtgagaacc
     5941 ttgtccctaa catccgaaac attattttta attaattcta ctgaaagaaa gacaacattt
     6001 ccagtttgtc cagctacaag ggtattcccg cgaacaataa aagtgtaagc atccacatat
     6061 ccagcacaaa acgtcaaaaa aagtgctaac cttttagact gacgtgatat ttttcttata
     6121 ggtaataacc tcattttacc tcccattgta ttttctctta gaaatattgt accattttct
     6181 ttctaaaaaa tcgtaggcta ccatttagat tttactatta gcataaaaat aataatagac
     6241 aactatttat ccaaaaatag atagatgtaa catgtttgca aacaaagcat acgaaccttt
     6301 agtaaaatca tttccatgaa actagaatag agccctctta gcaaaaatca ttattttaat
     6361 ttatttctaa tcactccttg acataaataa ctctcaccaa taaaagacta tgtcttaaaa
     6421 aaatggtata ataaaatcaa tacttgggct tgatggctat gctactaata acaattagga
     6481 gagaaaatca ggcacttgtt aacaacaagg attatcccct tgagatgaaa ggaactttag
     6541 aaatcttatg atgaacatgc aaaacatgat gcgtcaagca caaaaacttc aaaaacaaat
     6601 ggaacaaagc caagctgaac ttgctgctat gcaatttgtt ggcaaatctg ctcaagatct
     6661 tgtccaagcg accttaactg gcgataagaa agttgtcagc attgatttca atccagctgt
     6721 cgttgaccca gaggaccttg agactctttc tgatatgacc gttcaagcca tcaactctgc
     6781 tcttgaacaa atcgatgaaa ctaccaagaa aaaactgggt gctttcgctg ggaaattacc
     6841 tttctaaaaa caaggagcta gaacaatgct tgtcgataac aaaggctaag aaaggtgcaa
     6901 aaatgactct ataatatttg tagtgggtaa atcccctata gatattatgg agc
//
LOCUS       DAVKFH010000087         6870 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKFH010000087 DAVKFH010000000
VERSION     DAVKFH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.087"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..425
                     /locus_tag="VW006_001950"
     CDS             <1..425
                     /locus_tag="VW006_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9380742.1"
                     /translation="YFYREYGRSLKGQLRRGKVSGRRYQRISLVAGLTNGELIAPMTY
                     EEMMTSDFFEAWFQKFLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYS
                     PEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            511..1461
                     /locus_tag="VW006_001951"
     CDS             511..1461
                     /locus_tag="VW006_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9380743.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1638..2645
                     /gene="gap"
                     /locus_tag="VW006_001952"
     CDS             1638..2645
                     /gene="gap"
                     /locus_tag="VW006_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9380744.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2713..3549)
                     /locus_tag="VW006_001953"
     CDS             complement(2713..3549)
                     /locus_tag="VW006_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9380745.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3675..5870
                     /locus_tag="VW006_001954"
     CDS             3675..5870
                     /locus_tag="VW006_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9380746.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5923..6075
                     /gene="rpmG"
                     /locus_tag="VW006_001955"
     CDS             5923..6075
                     /gene="rpmG"
                     /locus_tag="VW006_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9380747.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6085..6261
                     /gene="secE"
                     /locus_tag="VW006_001956"
     CDS             6085..6261
                     /gene="secE"
                     /locus_tag="VW006_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9380748.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6316..6852
                     /gene="nusG"
                     /locus_tag="VW006_001957"
     CDS             6316..6852
                     /gene="nusG"
                     /locus_tag="VW006_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9380749.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2138 a         1196 c         1489 g         2047 t
ORIGIN      
        1 cttattttta tcgagaatat ggtcgctcat taaaaggtca gttaagaaga ggtaaagtat
       61 ctggaagaag atatcagagg atttctttgg ttgcaggtct aacaaatggt gagttaatcg
      121 ctccaatgac ttacgaagag atgatgacga gcgacttttt tgaagcttgg tttcagaagt
      181 ttctcttacc aacattaacc acaccatcgg ttattattat ggataatgca agattccata
      241 gaatgggtaa gttagaactt ttatgcgagg agtttgggca taaactttta cctcttcctc
      301 cctactcacc tgagtacaat cctattgaga aaacatgggc tcatatcaaa aagcacctca
      361 aaaaggtatt accaagttgc aatacctttt atgaggctct tttgtcctgc tcttgtttca
      421 attgactata gttcacggat acagttggga aagaagctat taaatatttg gagagtcctt
      481 ttgggatgaa catgtcaaat attactatag atgggcaaga aaagatgggc aagaaatgga
      541 tctgagtcaa acgatgcttc ttatgctcaa gtagtgagtc tttatgatga tacaagtatc
      601 tctgttagta ataatgagac ggacaaagtg ttgactggat ccctctatac tgaaacaaat
      661 gaacagggat taacgattcc aagcagttta ctaaaaaatt ggaatgaaca gacaggaaaa
      721 aatttgacaa ctaatgatct tattggcaaa tcagtctcag ccagcattgt agaaagtgct
      781 gccgaaacta gtaagattgc tcaatttcaa acgaagattg tacgtgtaat caatgatgaa
      841 gatgacatgg aggacagcaa cagtttcatg ctgtctcatc aaatggaaac gattttgaaa
      901 gaggctggat ttacgaaagc tgtatcttat tttatcttgg aactcaaaga tccatcacag
      961 acaaaagtag taacagaaga attacagaaa aataagaagt atactgtgct ttctcaacag
     1021 agagttcttg atattgtgat tacctttatt cgtgttattc agggattatt gattgtgctt
     1081 tcatcacaag ctattgtggt agcagcggtt atgattggta tcattattta catcaatatc
     1141 atgcaacgtt ccaaggaaat aggtgtcatg aaagcagttg gttatcagaa tcgtggtgtc
     1201 aaaggaattt ttatttacga ggctatctgg attgtaggca tcgccttgct gctggcattt
     1261 ttggttgcac aaggggtggg aagtttggcg aatgcgattg taagtcactt ttacccatcc
     1321 atcactaagg tttttgaatt aaatctttta tctgttttag gaactctagt tttcgctcta
     1381 ttacttggtt atgtctcagc ctacttcccg gcgcgtaaga ttagtaaaat ggatcctgta
     1441 gaatcgttac gctatgaata ataagggatt ccttgattta cagtcttaaa aagaaactca
     1501 catagaaaaa caatggccat acttgcaatt caactcaaat agttgtataa taggtttgtt
     1561 gaaaggtgat ttgtagtgat tcaagttact cttttcacaa taaaaatttc atgattttca
     1621 taaggaggaa atcactaatg gtagttaaag ttggtattaa cggtttcgga cgtatcggtc
     1681 gtcttgcttt ccgtcgtatc caaaacgtag aaggtgttga agttacacgc atcaacgacc
     1741 ttacagatcc agttatgctt gcacacttgt tgaaatacga cacaactcaa ggtcgtttcg
     1801 acggtactgt tgaagttaaa gaaggtggat tcgaagttaa cggtaaattc gttaaagttt
     1861 ctgctgaacg tgatccagag caaatcgact gggctactga cggtgtagaa atcgttcttg
     1921 aagctactgg tttctttgct aagaaagaag cagctgaaaa acaccttaaa ggtggagcta
     1981 aaaaagttgt tatcactgct cctggtggaa acgacgttaa aacagttgta ttcaacacta
     2041 accacgacgt tcttgacggt actgaaacag ttatctcagg tgcttcatgt actacaaact
     2101 gcttggctcc aatggctaaa gctcttcaag ataactttgg tgttgttgaa ggattgatga
     2161 ctactatcca cgcttacact ggtgaccaaa tgatccttga cggaccacac cgtggtggtg
     2221 accttcgccg tgctcgcgct ggtgctgcaa acatcgttcc taactcaact ggtgctgcaa
     2281 aagctatcgg tcttgtaatc ccagaattga atggtaaact tgatggatct gcacaacgcg
     2341 ttccaactcc aactggatca gttactgaat tggtagcagt tcttgaaaag aacgttactg
     2401 ttgatgaagt gaacgcagct atgaaagcag cttcaaacga atcatacggt tacacagaag
     2461 atccaatcgt atcttcagat atcgtaggta tgtcttacgg ttcattgttt gacgcaactc
     2521 aaactaaagt tcttgacgtt gacggtaaac aattggttaa agttgtatca tggtacgaca
     2581 acgaaatgtc atacactgca caacttgttc gtactcttga atacttcgca aagattgcta
     2641 aataattctt gagttgatag aaagcaaggc tttgtggtct tgctttttta tatggaaaaa
     2701 tggatgacac gatcatccat tcttttttaa ttctttttca aatgtatttg aaagggtagt
     2761 gaaagttagc ctctctaaag taagtgggtg ggtaaaggaa agtcggaagg catgaagcat
     2821 aagccggctt gtctttgatt tactattata gagagggtct cccaggatag gaagattatg
     2881 atgcaaaagg tgcacacgaa tctgatgggt tcgccctgtc tttagcttgc aatgagccaa
     2941 ggaagtcttg tttgagaatt gctttaatct gcttacatgc gtttcagcat atttcccatt
     3001 ttttgcatca actattcttt ttctacgatc atggcgatca cgtccaattt tgtctctgaa
     3061 aacaagttct tttctgttga tatttccatc aactagagcc caatattctc tagaaatctc
     3121 ttttttctcc aataagcgat tgagaatggg caggataaaa ggatttttgg caaagagaac
     3181 taagccactg gtttccatgt ccagacgatg aacgacatag caggtttggc caacataggt
     3241 actgacatgg ttaagaaggg caatttcgtt tggttgatta ccatgcgttt tcatcccctc
     3301 tggtttgttt acaataatca agtgttgatc ttgataaact tcctgcacta agtctgggtt
     3361 gccccaaggg atcgtctttt ggggataatc ttcctcgtca aaagtcaact ggcaaacatc
     3421 tccaggattt acgatttcgt tccagcggac ttcttcttga tttatcaaaa tatgtttctt
     3481 gattctcaaa aaatgacgga tttttctagg gatgaggagt tgttcctcaa gtaattgctt
     3541 taccgtcatt tgaggtagag aggcgggtaa tgtaaatgtg aattgcatac agatattgta
     3601 acaaaaaaag ccctatttgg ataggaaata gctaaattct tgtcttccta tgatgaagat
     3661 gataaaataa acgcatgaaa ttagataaat tatttgagaa atttctttct ctttttaaaa
     3721 aagaaacaag tgaactagag gactctgatt ctactatctt acgtcgctct cgtagtgatc
     3781 gaaaaaaatt agcccaagta ggtccgattc gaaaattctg gcgtcgttat catctaacaa
     3841 agattatcct tatactaggt ttgagtgcag gcttgctagt tggaatctat ttgtttgctg
     3901 tagccaagtc gaccaatgtc aatgatttgc aaaatgcctt gaaaactcgg actcttattt
     3961 ttgaccgtga agaaaaagag gctggtgcct tgtctggtca aaagggaacc tatgttgagc
     4021 tgactgacat cagtaaaaac ttgcagaatg ctgttattgc gacagaagac cgttctttct
     4081 ataaaaatga cgggattaac tatggccgtt tcttcttggc tattgtcact gctggacgtt
     4141 caggtggtgg ctctaccatt acccaacagc tggctaaaaa cgcctattta tcgcaggatc
     4201 aaactgttga gagaaaagcg aaagaatttt tccttgcctt agaattaagc aaaaaatata
     4261 gtaaggagca aattctaacc atgtacctta acaacgctta ttttggaaat ggtgtgtggg
     4321 gtgtagaaga tgcgagtaag aaatactttg gagtttctgc atcagaagtg agtctggatc
     4381 aagctgcgac tctggcaggg atgctcaagg ggccggaact gtataatccc ttgaattccg
     4441 tagaagattc tactaatcgg cgcgatactg tcttgaagaa tatggttgca gcaggatata
     4501 ttgataaaaa ccaagaaacc gaagctgctg aagttgatat gacttcgcaa ttgcacgata
     4561 agtatgaagg aaaaatctca gattaccgtt acccctctta ttttgatgcg gtggttaatg
     4621 aagctgtttc caagtataat ctaacagagg aagagattgt caataatggc taccgcattt
     4681 acacagagct ggaccaaaac taccaagcaa atatgcagat tgtttatgaa aacacatcgc
     4741 tatttccgag ggcagaggat ggaacgtttg ctcaatcagg aagtgtagct ctcgaaccga
     4801 aaacaggggg agttcgtgga gttgtcggtc aagttgctga caatgataaa actggattcc
     4861 ggaatttcaa ctatgcaacc caatcaaagc gtagtcctgg ttctacaatt aagcctttag
     4921 ttgtttatac gccagcagtt gaagcaggct gggctttgaa taagcagttg gataaccata
     4981 ccatgcagta taacagctat aaggttgata actatgcagg gatcaaaacg agtcgagaag
     5041 ttcctatgta tcaagccttg gcagaatcgc ttaatctacc tgctgttgcc actgttaatg
     5101 atttgggtgt tgacaaggct tttgaggcag gcgaaaaatt cggactcaac atggaaaagg
     5161 tcgaccgtgt tcttggtgtc gccttgggaa gcggtgttga aaccaaccct cttcaaatgg
     5221 ctcaagcata cgctgccttt gcaaatgaag gtttaatgcc tgaagctcat tttattagta
     5281 gaattgaaaa tgctagtgga caagttattg cgagtcataa aaattcacaa aaacgggtga
     5341 ttgataagtc tgtagctgac aagatgacca gtatgatgtt ggggactttc accaacggta
     5401 ccggtattag ttcatcgcct gcagactata ttatggcagg gaaaactgga acaactgaag
     5461 cagttttcaa tccggagtac acaagtgacc agtgggtaat tggttatact ccggatgtag
     5521 tgattagcca ctggcttggc tttcctacca ctgatgaaaa tcactatcta gctggctcta
     5581 cttcaaacgg tgcagctcat gtctttagaa acattgccaa taccatttta ccttatacgc
     5641 caggaagtac ctttacggtt gaaaatgctt ataagcaaaa tggaattgca ccagccaata
     5701 caaaaagaca agtacaaacc aatgataata gccagacaga tgataatttg tctgatattc
     5761 gagggcgtgc gcaaagtcta gtagatgagg ctagccgggc tatctcagat gcgaagatta
     5821 aggaaaaggc tcaaacaata tgggattcga tagtcaatct atttcgttaa gatgcttgtc
     5881 aaagcctagc tttcttgtta taatggataa gatggaggcg ttatggcact aaaaaaagca
     5941 agcctagctt gtgcggtttg tggttcgaga aactattcaa tcaagatcag cggaaacccc
     6001 aagcctacac gactagaagt aaataaattt tgtaagcatt gtggcaagta cactacacac
     6061 agagaaacga gataggagag agcgatgcgt tttattggag atatttttag acttcttaaa
     6121 gacacaacat ggccaactcg caaggaaagc tggagagatt ttcgttctat catggaatac
     6181 acagctttct ttgtagtaat tatttacatt tttgaccagt tgattgtttc aggtttgatt
     6241 cgatttatta acatttttta gaagattagt ggagttaatt acactagaaa tcttctattt
     6301 atgaaaggaa atatcatgga tagttttgat aaaggatggt ttgttttaca aacttattct
     6361 ggttatgaaa ataaggtgaa agaaaatcta ttacaacgtg cacaaaccta caatatgttg
     6421 gataatattc tacgcgttga aattccaaca caaacagtgc aagttgaaaa aaatggaaag
     6481 agaaaagaag tagaagaaaa tcgctttcca ggttatgttc ttgtagaaat ggtcatgaca
     6541 gatgaagctt ggtttgttgt tcgaaacaca ccaaatgtta caggatttgt cggatcacac
     6601 gggaacagat caaaaccaac tccattattg gaacaagaaa ttcgtgacat tttggtatct
     6661 atgggacaaa ctgttcaaga atttgatttc gatgttgaga ttggtcaaac tgtacgtatt
     6721 attgatggtg cttttgcaga ctacactggt aagattacag aaattgataa taacaaagtg
     6781 aaaatgatta tctctatgtt tggtaatgac acagttgcag aagtaaacct aaaccaaatc
     6841 gcagaattat aaccctgaga gaggctggag
//
LOCUS       DAVKFH010000088         6709 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKFH010000088 DAVKFH010000000
VERSION     DAVKFH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.088"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(28..291)
                     /locus_tag="VW006_001958"
                     /pseudo
     CDS             complement(28..291)
                     /locus_tag="VW006_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            489..2255
                     /gene="patB"
                     /locus_tag="VW006_001959"
     CDS             489..2255
                     /gene="patB"
                     /locus_tag="VW006_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9380750.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2394..3083
                     /locus_tag="VW006_001960"
     CDS             2394..3083
                     /locus_tag="VW006_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9380751.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3087..3266
                     /locus_tag="VW006_001961"
     CDS             3087..3266
                     /locus_tag="VW006_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380752.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3253..4602
                     /locus_tag="VW006_001962"
     CDS             3253..4602
                     /locus_tag="VW006_001962"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9380753.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4726..6186
                     /locus_tag="VW006_001963"
     CDS             4726..6186
                     /locus_tag="VW006_001963"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9380754.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            6200..6631
                     /locus_tag="VW006_001964"
     CDS             6200..6631
                     /locus_tag="VW006_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9380755.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT         2106 a         1289 c         1440 g         1874 t
ORIGIN      
        1 gcatgctttg gcagcttgta tgagtaccta ccgcagatga ggggaacccc gtttgaccat
       61 tctttctgct aaatcaatct gacctgactg ataaatagaa gaatccagtc cagcgaaagc
      121 ttgtaattga gcaggattat caaaggcatg aatatttcga atctcggcta aaatgaccgc
      181 ccctaaacga cccccaatcc cagtaaccgt cgtgatgacc gagttgaact cagccatcaa
      241 gtcattgaca cattttttcg ccttgtcaat gagcctcttg taatgtttga tgttttcatt
      301 acaagagata aaacgtttat gcgttatcaa actcattacc aattgaaaca aaaagctgtg
      361 gttagatcct ttcggaaatt gtcaagcgat tggaggaaat gaactaatcc acagcggctt
      421 attccaagta taccacttgg gctttggcag tagctaactg cgctaaatat aatataagga
      481 ggagtaaaat gaagacagtt caattttttt ggcattattt taaggtctac aagttctcat
      541 ttgtagttgt catcctgatg attgttctgg cgacttttgc ccaagccctc tttccagtct
      601 tttctggaca agcggtgacg cagctagcca atttagttca agcttatcaa aatggcaatc
      661 cagaacttgt atggcaaagc ctatcaggaa tcatggtcaa tcttggcctg ctggttttgg
      721 ttctatttat ctctagtgta atatacatgt gtctcatgac gcgcgtgatt gcagaatcga
      781 ccaacgagat gcgcaaaggc ctctttggta agcttgctca gttgacggtt tctttctttg
      841 accgtcgaca agatggcgat atcctgtctc attttaccag tgatttggat aatatcctcc
      901 aagcctttaa cgaaagcttg attcaggtca tgagcaatat tgttttatac attggtctga
      961 ttcttgtcat gttttcgaga aatgtgacgc tggctctcat caccattgcc agcactccat
     1021 tggctttcct tatgctgatt ttcatcgtga aaatggcacg taaatacacc aacctccagc
     1081 agaaagaggt agggaagctc aacgcctata tggatgagag catctcaggc caaaaagccg
     1141 tgattgtgca aggaattcaa gaggatatga tggcaggatt tcttgaacaa aatgagcgcg
     1201 tgcgcaaggc aacctttaaa ggaagaatgt tctcaggaat tcttttccct gtcatgaatg
     1261 ggatgagcct gattaataca gccatcgtca tctttgctgg ttcggctgta cttttgaatg
     1321 ataagtctat tgaaacaagt acagccctag gtttgattgt tatgtttgca caattttcac
     1381 agcagtacta ccagcctatt atccaagttg cagcgagttg gggaagcctt cagttggcct
     1441 ttactggagc tgaacgaatt caggaaatgt ttgatgcaga ggaggaaatc cgacctgaaa
     1501 aggctccaac cttcactaag ttgcaagaaa gtgttgaaat cagtcatatc gatttttcat
     1561 acttgcctga taaacctatt ttgaaagatg tcagcatttc tgcccctaaa ggccagatga
     1621 cagcagttgt tgggcctaca ggttcaggaa aaacgactat tatgaacctc atcaatcgct
     1681 tttatgatgt tgatgctggt ggtatttatt ttgatggtaa agacattcgt ggctatgact
     1741 tagatagtct tagaagcaag gtgggaattg tattgcaaga ttcggtcttg tttagcggaa
     1801 cgattcgaga caatatccga tttggtgtgc cagatgctaa tcaggaaatg gttgaggtag
     1861 cagcaaaagc aacccacatt cacgactata tcgaaagttt gcctgataag tacgatactc
     1921 ttattgatga tgaccagagc atcttttcaa cagggcagaa gcaattgatt tcaatcgctc
     1981 gaaccctgat gacagatcca gaagttctca ttctcgatga agcaacttca aacgtagata
     2041 cggtgacgga aagcaagatt cagcatgcca tggaggtggt tgtagcaggt agaactagtt
     2101 tcgtcattgc ccaccgcttg aaaaccattc tcaatgcaga tcagattatt gtccttaaag
     2161 atggagaagt cattgaacgt ggtaaccacc atgaactttt gaagctaggt ggcttttatt
     2221 cagaactcta tcacaatcaa tttgttttcg aataagaaag aagttgtcct atgtgggcag
     2281 ctttttcttg tccataaaaa atgtttatca cagccttaaa aaaaacatat tagacgaaag
     2341 tcattttgag tgatatgata ggactatcgt tagtattcga aaggagaggc atcatggcta
     2401 gaacggttgt aggagttgct gcaaatctat gtcccgtaga cgcagaaggc aaaatcattc
     2461 attcatctgt atcttgtaga ttcgcagaga tcattcgtca agtcggtggt ctccctttag
     2521 tcattcctgt tggtgatgag tcagttgtac gtgattatgt ggaaatgatt gacaaactca
     2581 ttttgacagg aggccaaaat gttcatcctc agttttatgg agagaaaaag accgtcgaaa
     2641 gcgatgatta caatctggtc cgtgacgaat ttgaattggc actcttgaag gaagcgcttc
     2701 gtcagaataa accaattatg gcaatctgtc gcggtgtcca acttgtcaat gttgcctttg
     2761 gtggaaccct caatcaagaa atcgaaggtc actggcaagg actacctttc gggacatctc
     2821 actctattga aacagtagaa ggaagcgtgg tggctaagct atttgggaaa gaaagtcagg
     2881 tcaactcagt ccatcgtcaa agcattaaag atttggcacc taatttccgt gtaactgcta
     2941 ttgattcaag agaccaaact attgaagcga ttgagtctat cgacgagcac cgcattatcg
     3001 gtttacagtg gcatccagag tttctggtta atgaagaaga tggcaattta gaattatttg
     3061 agtatttatt gaatgaactg taacgattgg aacatctggt cgttctttct tttatttttt
     3121 caaaattttt agaatgtggc attttacgca aacgtttgaa ttctgataaa aattggagaa
     3181 attcgacaaa aaaacttgaa aaaaacgaag gtaagcgtta tgatagaaaa gaagaaatat
     3241 tggaggaaga acatgtcaca tattaaattt gattattcaa aagttttaga caaatttgtt
     3301 gcaccacatg aagtggaata catgcaatca caagtaacag cagcagatga attgatccgt
     3361 aaaggaactg gtgctggtag cgacttcttg ggatggttgg accttcctga aaaatacgac
     3421 cgcgaagaat ttgaccgcat cttgaaagct gctgagcaaa tcaagtcaga cagcgatgtt
     3481 ttggttgtga tcggtattgg tggatcttac cttggtgcaa aagcagcaat cgacttcttg
     3541 aatcaccatt ttgctaactt gcaaacaaaa gaagaacgca aagctccaca aatcctttac
     3601 gcaggaaact caatctcatc tacttacctt gctgacttgg tagagtatgt tgcagacaaa
     3661 gacttctcag taaacgtaat ttctaaatca ggtacaacaa ctgaaccagc gattgctttc
     3721 cgtgtcttta aagaactctt ggttaagaaa tacggtcaag aagaagctaa caaacgtatc
     3781 tatgcaacaa ctgaccgcca aaagggtgct gttaaggttg aagcagacgc taacggttgg
     3841 ggaacatttg ttgttccaga tgatatcggt ggacgcttct cagtattgac agccgttggt
     3901 ttgcttccaa tcgcagcatc aggagctgac atcaaagctc ttatggaagg tgcgaatgca
     3961 gctcgcaaag actacacttc agacaaaatc tctgaaaacg aagcttacca atacgcagtc
     4021 gttcgtaaca tcctttatcg taaaggctat gcaactgaga tcttggtaaa ctatgagcca
     4081 tcacttcaat acttctcaga atggtggaaa caattggctg gtgaatcaga aggaaaagac
     4141 caaaaaggta tctacccaac ttcagccaac ttctcaactg acttgcactc acttggtcaa
     4201 tttatccaag aaggaactcg tatcatgttt gaaacagttg tccgtgttga caaacctcgt
     4261 aaaaacgtgc ttattcctac tttggaagaa gaccttgacg gacttggtta ccttcaagga
     4321 aaagacgttg actttgtaaa caaaaaagca actgacggtg ttcttcttgc ccacacagat
     4381 ggtgatgtac caaacatgta tgtgactctt ccagagcaag acgctttcac tcttggttac
     4441 actatctact tcttcgaatt ggcaattgcc ctttcaggtt acttgaatgc tatcaaccca
     4501 tttgaccaac caggtgttga agcttataaa cgtaacatgt ttgcccttct tggaaaacca
     4561 ggatttgaag aattgagcaa agaacttaac gcacgtctat aatagaagaa aagagtggtt
     4621 tgcccactct ttttactctc tttatccata gaaattggac tcagccaaga cttgtgatat
     4681 aatatagaaa gcaaaaaggc agacgcctag ataataggag aaactatgtc aaaagatatc
     4741 cgcgtacgtt acgcaccaag tccaacagga ctactacaca tcggaaatgc ccgtacagca
     4801 ttgtttaact acttgtatgc gcgccatcat ggtggaacat ttctcatccg tatcgaagat
     4861 actgaccgta aacgccatgt cgaggatggt gaacgttcac aacttgaaaa ccttcgctgg
     4921 ttaggcatgg attgggatga aagtccagaa tcacatgaga attatcgcca gtctgagcgt
     4981 ttggacttgt atcaaaaata cattgaccaa ctattagctg aaggaaaagc ctataaatct
     5041 tacgttacag aagaagagtt ggcagctgaa cgtgaacgtc aagaagtagc tggcgaaaca
     5101 ccacgctaca tcaatgaata ccttggtatg agtgaagaag aaaaagcggc ttatatcgca
     5161 gaacgtgaag cagctggtat cataccaaca gttcgtttgg ctgtcaatga gtcaggtatc
     5221 tacaagtggc atgatatggt caaaggtgat atcgaatttg aaggtggcaa tatcggcggt
     5281 gactgggtta tccaaaagaa agacggttac ccaacttaca actttgccgt tgttatcgat
     5341 gaccacgata tgcaaatctc tcatgttatc cgtggagatg accatattgc taatacacca
     5401 aaacagctta tggtctatga agctcttggt tgggaagctc cagagtttgg tcacatgacc
     5461 ttgattatca actctgagac tggtaagaag ttatctaaac gtgatactaa tacacttcaa
     5521 tttatcgaag attaccgaaa gaaaggttac cttccagaag cagtctttaa ctttattgct
     5581 cttcttggtt ggaacccagg tggtgaagat gagattttct ctcgtgaaga actcattaaa
     5641 ctttttgatg aaaaccgcct cagcaagtca ccagcagcct ttgatcagaa aaaattagac
     5701 tggatgagca atgattatat caagaatgca gaccttgaaa ccatctttga aatggcaaaa
     5761 ccattcttag aggaagcagg ccgtttgact gacaaggctg aaaaattagt tgagctctat
     5821 aaaccacaaa tgaaatcagt agatgagatt atcccattga cagatctttt cttctcagat
     5881 ttcccagaat tgacagaagc agagcgcgaa gtcatgacgg gtgaaacagt tccaacagtt
     5941 cttgaagcat tcaaagcaaa acttgaagcg atgacagatg atgaatttgt gacagaaaat
     6001 atcttcccac aaattaaagc agtccaaaaa gaaacaggta ttaaagggaa aaatcttttc
     6061 atgcctattc gtatcgcagt ttcaggcgaa atgcatgggc cagaattacc agatacaatt
     6121 ttcttgcttg gacgtgaaaa atcaattcag catatcgaaa acattctaaa agaaatctct
     6181 aaataaagag gatacaataa tggacatctg ggaaaagatg tacgaagaag cacagaaact
     6241 atacaatcca catgaagtat cagactttgt ttatgccaat catgttgtag ctgcagtaga
     6301 agcaggagat ggacaaatat ttacaggatt ctgtatggag ggaacctgtg gtgttttcca
     6361 tctctgcgca gaacgggcga ccctcttcaa tatgtaccaa ttttcaggac aaactaaggt
     6421 taagaaagta ttagcctttc gagacaaacc accttatggt ggaagttcag ccatgccttg
     6481 cggtgcttgt agagaattcc ttttagagct taatgctgaa aataaagatg cagaattcat
     6541 gatggactat aatataagaa aaacagttaa agtcgcagaa ctaatccctt attggtgggg
     6601 agaagaacgt gcttctaagt ttaatgagtg atagaagagt cagtataatt ctggctcttt
     6661 ttactataag ttgaaaaaat aaaaaaaatt agaaaaagta gttgacaaa
//
LOCUS       DAVKFH010000089         6595 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKFH010000089 DAVKFH010000000
VERSION     DAVKFH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.089"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(231..860)
                     /locus_tag="VW006_001965"
     CDS             complement(231..860)
                     /locus_tag="VW006_001965"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9380756.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(862..1644)
                     /gene="thiM"
                     /locus_tag="VW006_001966"
     CDS             complement(862..1644)
                     /gene="thiM"
                     /locus_tag="VW006_001966"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9380757.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPE
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(1881..2549)
                     /locus_tag="VW006_001967"
     CDS             complement(1881..2549)
                     /locus_tag="VW006_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9380758.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLIMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(2764..2852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3106..4242)
                     /gene="lctO"
                     /locus_tag="VW006_001968"
     CDS             complement(3106..4242)
                     /gene="lctO"
                     /locus_tag="VW006_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9380759.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLRLY"
     gene            complement(4867..6357)
                     /gene="lysS"
                     /locus_tag="VW006_001969"
     CDS             complement(4867..6357)
                     /gene="lysS"
                     /locus_tag="VW006_001969"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9380760.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(6485..>6595)
                     /locus_tag="VW006_001970"
     CDS             complement(6485..>6595)
                     /locus_tag="VW006_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9380761.1"
                     /translation="DFINTYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         1957 a         1407 c         1132 g         2099 t
ORIGIN      
        1 taaagcaccc caaatgtctt tattatttaa ttatgtgatt attataacac acattttata
       61 ctagttcaag aaattgaact ggaaatacag ccttgcactc acaaagacag cagatctttc
      121 ttttgcaaaa aacaaatgac ctgtttgatg aattagccat tcaagctgaa tctggacata
      181 gctttttaaa aaaggaaaat cctacttact tagaatccaa ggatagatat ctattgttca
      241 ctcatttccc gaacagtttt ttctatattt tttgcatacg atattgccga aatgattgaa
      301 acgccatcca tattggtctt tataatgtct ttaatatgtt tcgtctgtat cccaccaatt
      361 gcaactaaag gcatttgtgg caatagtttt ctcatcaatt caagaccttc ataacctata
      421 gcaccaccag catcatcttt tgactgggta tcaaatacag gcccaacacc tacataatct
      481 acatattcaa cttttgattg ttgaaattct tcttcgtttc ttatagaaag accaattatt
      541 ttatctggca tcaattttct aatttcatca acaccaatat catcttgacc tacatgtacg
      601 ccatcggcgt caatttccat tgctaaatct atatcgtcat taacgataaa tggaacattg
      661 tattttttac aaagttcttt aatttggata gctagctcaa gtttttctaa gccttctaaa
      721 gcaccctcac ctttttctcg aaattgaaat aaggttatac caccttttaa ggcttcctca
      781 acgactgtat atagattttt tccttggcaa gtagtcgttc cacaaataaa atatagtttt
      841 agtaattctt tatgaaacat cttacttcac tcttttgaat tcctttacat cttcatctgt
      901 aatctcgtat aaggcattta taaattcaac tttaaatgtc ccaggaagat gtccatttgg
      961 acgtttttct gccatttctc cagcgatatt gtaaactaac attgctgttt ctaatgattt
     1021 taattcttga cctttttcca gtccgataaa acttgctact acagctccta ataaacatcc
     1081 agttccaata acttttggca tcatggcact accattatga atcgttacca cttctccatt
     1141 taccgcaata gcatccactt cacctgttac tactattgga atattgaact tctcatttgc
     1201 tgctagagca atctcgtcaa tattatctac tcccgcacta tctactcctt tagatgccac
     1261 atctattcct actaaagagg caatctctcc agcattcccc ctaatcgcag ctagtttgta
     1321 attgttgatt aaatcatctg ctactttttt tctatattct cctgctccac aggctacagg
     1381 atctaaaact gctgggacat tatatttctc tgcaattttc agagcagctt ggtataattt
     1441 ccaattttca tctgtcaatg ttcctatgtt tattaataaa ccaccagcat actttaataa
     1501 gtcctctaaa tcttctggaa actcactcat ggctggtgag gcgcccagtg ctactaatcc
     1561 atttgctgtg aaatttttta ctacatcatt ggtgatgcaa atgaccaaag gtgctttttc
     1621 ttttaataat tttaaacttg tcatattgaa atccttcctt ttcactttat acgatctact
     1681 aatttcgatt tatctttagt tgagaatttt tttcatttac attgaatgat ttatactcaa
     1741 tgaaaatcaa agagcaaact aggaggctaa ccgcaggttg ctcaaaacac tgttttgagg
     1801 ttgtggatag aactgacgtg gtttgaagag attttcgaag agtcttacct catcaaattt
     1861 gtaaatatca tgagccttct ctagacatcg taaccaatat caaaaaaagc taattctaaa
     1921 gcgactgctt gattccagcg ttgctgaagt tctgtcaaat cttctcgatt tttaccgaca
     1981 cgattgagtt cgtcaaccag aaattgaacc cactctgcaa agaaaggacc tctgtggaga
     2041 ttgatccatt ccgaatgaat atagacttca ggtaaagcca aatctttaga accccagtct
     2101 aaatagagac cttctgcaat gaccagcata atcaaaagat gggcatagtc tgatgaagcc
     2161 actgccgaat acattagatc ctgaaaggct tttgttacag ggtgcaaagt cacttctaga
     2221 tagtcattct ctgctacttt taactcttta aaagcctttt ggaaataacc atcttcatct
     2281 gcttcaagaa agcctagttg cttggcaaaa cgaagcttgg attcaagttt atctgcgtga
     2341 gctacgcagg cacccagcat ggataagaag gcatcaaaga agtgataatc ttgaatcaga
     2401 tagtccttta agaccttatt ctcaattgtc cccgcaaaaa gttccttaac aaaacgatga
     2461 ttgattgcag cctgccaatc cttctgactg ctttttaata attctccaac agtcaaacct
     2521 ggctgaaatg catagtcttg tgtttccata tttacttctc ctctctttac ttgttagtaa
     2581 ttaataaaac accaagaaat atcaagcaaa atcgtaattc cacttgatcc ttttaaagca
     2641 catcgagagc atttgcagag agctaactaa acaagcctat ccagtttata taaacaaaaa
     2701 actccaatta caatcaagaa ttagagttga cttacaagat tagatcgttc atttcgtcat
     2761 acgaaaaaac gtttcacatt tcccttcgcc agtcttaact gtatcaggtt caatgggtat
     2821 catctcagct taaagcaccc caaatgtctc tattatttaa ttactgaacc agtatagcaa
     2881 aaaatgaaag ccctagcaag atatttgacc gaaaaatatc tttatatata gtagattgaa
     2941 actagaatag tacacatcta cttctaaaac attgttaaaa atcgatttaa ctgtcctgat
     3001 tgatttgtcc tgtttttatt ccattttact atggttttcg atagcgattt attcttccaa
     3061 tacacgaaga aaaacctcca cattcagtgg aggcaatctc ttttatcaat acaatcttaa
     3121 gtcacgaggg tcaactggga aagttgggtt gtatgggttg tgacggagtt tgaagtgttt
     3181 aacatcttca atagtctgag ttccagacaa ctgcataact gtcttcaatt ccgcattcaa
     3241 gtgttcaaag acttgacgca caccaacact accgccgaga gccaagccat agataaccgg
     3301 gcgaccaata gctaccaagt ctgctcctga agccaaggct ttaaagacat gttgaccacg
     3361 acgaacacca gagtcaaaga caattggcac acgtttatca actgcttctg ccacttcttg
     3421 aagcgagtca aaggcagctg gtccaccgtc gatttgacga ccaccgtggt tggttaccca
     3481 gataccagaa gctcctgcag caagcgaacg ttcaacgtcc tcacggcatt gtggtccctt
     3541 gacatacaca ggaagtcctg agtattcagc gataaattct acatcacgtg gagacaagcg
     3601 ttgtttagct gatttgtaaa caaagtccat tgatttacca gcaccttctg gcaggtattc
     3661 ttcaacaatc ggcatgccaa ctgggaagac aaaaccatta cgcttatcca cttcacgatt
     3721 cccccctaca gtagcatctg ccgtcaagac aatcgcttta taaccttcag ccttcacacg
     3781 atccatgatg tggcggttga taccgtcatc cttactaaag taaaattgga accaatgggg
     3841 tgtcccttga agggcttcag aaatctctgg aaggtcaaca gtagagtaag aactggttgt
     3901 ataaagagaa ccaaactcat gcacaccacg cgcagtcgcc acttccccct gttcattcgc
     3961 taatttatgg gccgcaacag gcgccataat gattggtgaa gatagttttt cacctgcaaa
     4021 ttcaatctct gtacttggat tttcaacatc gcaaagtgta tgaggaacga tgagtttgtg
     4081 gttaaaggcg cggatattct ctcttaaagt gaaagtatct tccgccccac tagcgatata
     4141 gccaaatgct gctttaggaa taacttgttg cgccattggc tccaaatcat aggtattgat
     4201 gaagtctaca tgaccttctg cattgcttgt tttgtatgac ataaaatgcc ctccttgatt
     4261 aagtaagcgt ttgcttttta ctacacaact ataataactc ttttttcaat cattttcaag
     4321 agttttgctt aaaaaccatc ttattttttc atacaatata ctagccctac tttccttaaa
     4381 ctctaaacct tagtctatat taagtgaatc gttgagattt tttgctgcca ggcatttgaa
     4441 ctactttttt atttgcggat tctatctact aatactataa aaaaacaagt gaataagaca
     4501 ccagaacatc ctctaatagg acctatggac ttatatcagt cccatatata ctgtttaagc
     4561 aagatactat cctagttgac gctaacttcc tatcccactt attaattttt tatttatagt
     4621 gaatctgaac aaatcaattg gggaatccaa gtcaatttct agtacttttt gaacagtttc
     4681 agcgtactat cgtaaaatga aataaaacat gcacaaattg cttagggaat ttaaaccaat
     4741 ttctaacaat gtcttagaaa tcaaagtgta ctatttcaac ttcaatatac tacacttcat
     4801 tttctcctta aattcagtct ctttttgaat caaaaaatcc cctggtaaga gccagaggat
     4861 aagaatttat ttcattgttg ggaagagcaa tacatcacgg atagttgttg tatcagtgag
     4921 gagcatgcag agacggtcga taccgattcc caaaccacct gttggtggca taccgtattc
     4981 aagagcttca atgtagtcat agtcgattcc tgtcgcttca tcatcaccaa gttctttggc
     5041 tttagcttgg gcttcaaaac ggctaagttg gtcgattggg tcgttcaact cagtaaaggc
     5101 attaccgtac tccttagtca tgataaagag ctcgaaacgg tcagtaaagc gttggtcttc
     5161 aggatttttc ttagcgagtg gagatacagc tactggatgt ccatagacaa aggttggttg
     5221 gattaaagtt tcttcaacaa actcttcaaa gaaggcattg atgatgtgac caacctcagt
     5281 gtagtgtttc tcaactggaa ctttcttctc agcagcgata gctttagctt cttccaaagt
     5341 catgtcttgc cagaaatcga caccagtaat ttctctgata gcatccacca tatgaacacg
     5401 cttaaatggt tcgttaatct tgatttcagt accttggtag ttgactgggc catcaccttt
     5461 gactgattta gcagcgtgtt ggataatgcc ttcagtcaag tccatgatgt cttggaagtc
     5521 tgcataagct tggtaaactt cgatagaagt gaactcaggg ttatgagtag cgtccattcc
     5581 ttcgttacgg aagatacggc caatttcata gacacgttcc ataccaccca cgataaggcg
     5641 ttttaagtga agctcagtcg cgatacgaag caccatgtca atgttttggg cattgtggtg
     5701 ggtgataaat ggacgggcag cagcaccacc ggcttcatta tgaagaacag gtgtttccac
     5761 ttcaaggaat cctttttggt caaggtaacg acggatttca gagatgattt ttgaacgagt
     5821 gacaaagcgt tcaaagcttt cacgattaga aatcaagtca aggtaacgtt tacggtaaat
     5881 tgtttcaacg tctgtcaaac catggaattt ctcaggaaga ggacgaagag ccttagacaa
     5941 gtgtgtgatg tgggttgcct tgatagagag ttctcccata tccgtacgca tcacttcacc
     6001 ttcgacacca aggaagtcac caaggtctgc ttttttgaag atttcgtagt tttcttcacc
     6061 gacagcatcc ttacgaacgt agatctgaat ctggccttcg cggtcttgaa ggtgggcaaa
     6121 accaactttt cctttaccac gtttggttac caagcgtcct gcgatagtag ctgtttcgtt
     6181 tttatcgtgt aattgttctt tatcgaggtt ggtatattta tcttttaatt cttgtgaatt
     6241 tgcagtacgt tcaaaacgtt ttccgaaagg atcgattcct tgttcgcgga gcgcagccat
     6301 tttttcacgg cgaacgatct gctggtcatt tagttcttcc atatgttctg tagacatggg
     6361 atcctcctag ttttactcaa aattgaatac gatgagtcat tttttgcgat actcccattt
     6421 tatcataaat aagcaagaaa ttcacggata atctttaaaa actaaaaaga acctgacgct
     6481 aaaatcactg gaaagaagtg aaagtatctc ccgccccact agcgatatag ccaaatgctg
     6541 ctttaggaat aacttgttgc gccattggct ccaaatcata ggtattgatg aaatc
//
LOCUS       DAVKFH010000090         6540 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKFH010000090 DAVKFH010000000
VERSION     DAVKFH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.090"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            208..471
                     /locus_tag="VW006_001971"
     CDS             208..471
                     /locus_tag="VW006_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9380762.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            477..2210
                     /gene="ptsP"
                     /locus_tag="VW006_001972"
     CDS             477..2210
                     /gene="ptsP"
                     /locus_tag="VW006_001972"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9380763.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2465..4915
                     /gene="phtA"
                     /locus_tag="VW006_001973"
     CDS             2465..4915
                     /gene="phtA"
                     /locus_tag="VW006_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9380764.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            5073..>6540
                     /locus_tag="VW006_001974"
     CDS             5073..>6540
                     /locus_tag="VW006_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9380765.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFE"
BASE COUNT         2222 a         1252 c         1360 g         1706 t
ORIGIN      
        1 aatgattatc ctataaattg tttttgaaga aactatatcc tgtgttttgt tatctaggat
       61 agaagatttt cagcaagtta tctgaaagga aattgaagga aaatccagaa aatgcttgaa
      121 aaaaatccta gaagatggta taatactaaa ttgtaagggt tatcacatat aactcaaaaa
      181 aagaaagaac aaaaggagag tcaaactatg gcttctaaag atttccacgt agtggcagaa
      241 acaggtattc acgcacgtcc agcaacattg ttggtacaaa ctgctagcaa atttgcttca
      301 gatatcactc ttgagtacaa aggtaaatca gttaacctta aatcaattat gggtgttatg
      361 agtcttggtg ttggtcaagg tgctgacgta actatctcag ctgaaggtgc agatgcagat
      421 gacgctatcg ctgcaatctc agaaacaatg gaaaaagaag gattggcata agggaaatga
      481 cagaaatgct taaaggaatc gcagcatctg acggtgttgc agttgcaaaa gcatatctac
      541 tcgttcagcc ggatttgtca tttgagacta ttacagtcga agatacaaac gcagaagaag
      601 ctcgccttga tgccgctcta caggcatcac aagacgagct ttctgttatt cgcgagaaag
      661 cagtaggtac gctcggtgaa gaagcagctc aagtttttga tgctcactta atggttcttg
      721 ctgacccaga aatgatcagc caaatcaagg aaactatccg tgcgaagaaa gtgaatgcag
      781 aagcaggtct gaaagaagtt acagatatgt ttatcactat ctttgaaggc atggaagaca
      841 acccatacat gcaagaacgc gcagcggata tccgcgacgt gacaaaacgt gtattggcaa
      901 accttcttgg taaaaaattg ccaaacccag cttctatcaa tgaagaagtg attgtgattg
      961 cgcatgactt gactccttca gatacagctc aattggacaa aaactttgta aaagcttttg
     1021 taaccaacat tggtggacgt acaagccact cagctatcat ggcacgtaca cttgaaattg
     1081 ctgctgtatt aggtacaaac aacatcactg aaatcgttaa agacggtgac atccttgctg
     1141 ttaacgggat cactggagaa gtgattatca acccgacaga tgaacaagcg gcagaattta
     1201 aagcagctgg tgaagcctat gcgaaacaaa aagctgaatg ggcacttttg aaagatgctc
     1261 aaacagtgac tgctgacggt aaacacttcg agttggctgc caatattggt actccaaaag
     1321 acgttgaagg tgttaacaac aacggtgcag aagctgttgg actttaccgt acagagttct
     1381 tgtacatgga ttctcaagac ttcccaactg aagatgagca gtatgaagca tacaaggctg
     1441 ttcttgaagg aatgaacggt aaacctgttg tcgttcgtac aatggatatc ggtggagata
     1501 aggaacttcc ttacttcgat atgcctcacg aaatgaaccc attccttgga ttccgtgctc
     1561 ttcgtatctc tatctctgag actggagatg ctatgttccg cacacaaatc cgtgctcttc
     1621 ttcgtgcgtc tgttcacggt caattgcgta tcatgttccc aatggttgcg ctcttgaaag
     1681 aattccgtgc agcgaaagca gtctttgatg aagaaaaagc aaaccttctt gctgaaggtg
     1741 ttgcagttgc ggataacatc caagttggta tcatgatcga gattcctgca gcggctatgc
     1801 ttgcagacca atttgctaaa gaagttgact tcttctcaat tggtacaaac gacttgatcc
     1861 aatatacaat ggcagcagac cgtatgaacg aacaagtttc atacctttac caaccataca
     1921 acccatcaat cctacgcttg attaacaatg tgatcaaagc agctcacgct gaaggtaaat
     1981 gggctggtat gtgtggtgag atggctggtg accaacaagc tgttccactt cttgtcggaa
     2041 tgggcttgga tgagttctct atgtcagcaa catctgtact tcgtacacgc agcttgatga
     2101 agaaactcga cacagctaag atggaagagt acgcaaaccg tgcccttaca gaatgctcaa
     2161 caatggaaga agttcttgaa cttcaaaaag aatacgttaa ttttgattaa tcgaaaagtc
     2221 cctgcaactc agttacaggg actttttttg atattttaaa aagaattttc aagaaaatct
     2281 ttcttataaa aagtccaacc ttgaaaaagt agtggtcaga acaaaaaata cttaaatggt
     2341 tcataaaatt cttgacaagt tggagattta ggagtaaact attaaccagt taagtaatag
     2401 agaggagttt ctgcaattta gaaatgaatt gcaactagaa atatcaaata gaaagagagt
     2461 ttcgatgaaa attaataaga aataccttgt tggttctgcg gcagctttga ttttaagtgt
     2521 ttgttcttac gagttgggac tgtatcaagc tagaacggtt aaggaaaata atcgtgtttc
     2581 ctatatagat ggaaaacaag cgacgcaaaa aacggagaat ttgactcctg atgaggttag
     2641 caagcgtgaa ggaatcaatg ctgagcaaat cgtcatcaag ataacagacc aaggctatgt
     2701 cacttcacat ggcgaccact atcattatta caatggtaag gttccttatg atgcgatttt
     2761 cagtgaagaa ttactcatga aagatccaaa ctataagcta aaagatgagg atattgttaa
     2821 tgaggtcaag ggtggatatg ttatcaaggt agatggaaaa tactatgttt accttaagga
     2881 tgctgcccac gcggataacg tccgtacaaa agaggaaatc aatcgacaaa aacaagaaca
     2941 tagtcaacat cgtgaaggtg ggactccaag aaacgatggt gctgttgcct tggcacgttc
     3001 gcaaggacgc tatactacag atgatggtta tatctttaat gcttctgata tcatagagga
     3061 tactggtgat gcttatatcg ttcctcatgg agatcattac cattacattc ctaagaatga
     3121 gttatcagct agcgagttgg ctgctgcaga agccttccta tctggtcggg gaaatctgtc
     3181 aaattcaaga acctatcgcc gacaaaatag cgataacact tcaagaacaa actgggtacc
     3241 ttctgtaagc aatccaggaa ctacaaatac taacacaagc aacaacagca acactaacag
     3301 tcaagcaagt caaagtaatg acattgatag tctcttgaaa cagctctaca aactgccttt
     3361 gagtcaacga catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg
     3421 aacagctaga ggtgttgcag tgccacacgg agatcattac cacttcatcc cttactctca
     3481 aatgtctgaa ttggaagaac gaatcgctcg tattattccc cttcgttatc gttcaaacca
     3541 ttgggtacca gattcaaggc cagaacaacc aagtccacaa ccgactccgg aacctagtcc
     3601 aggcccgcaa cctgcaccaa atcttaaaat agactcaaat tcttctttgg ttagtcagct
     3661 ggtacgaaaa gttggggaag gatatgtatt cgaagaaaag ggcatctctc gttatgtctt
     3721 tgcgaaagat ttaccatctg aaactgttaa aaatcttgaa agtaagttat caaaacaaga
     3781 gagtgtttca cacactttaa ctgctaaaaa agaaaatgtt gctcctcgtg accaagaatt
     3841 ttatgataaa gcatataatc tgttaactga ggctcataaa gccttgtttg aaaataaggg
     3901 tcgtaattct gatttccaag ccttagacaa attattagaa cgcttgaatg atgaatcgac
     3961 taataaagaa aaattggtag atgatttatt ggcattccta gcaccaatta cccatccaga
     4021 gcgacttggc aagccaaatt ctcaaattga gtatactgaa gacgaagttc gtattgctca
     4081 attagctgat aagtatacaa cgtcagatgg ttacattttt gatgaacatg atataatcag
     4141 tgatgaagga gatgcatatg taacgcctca tatgggccat agtcactgga ttggaaaaga
     4201 tagcctttct gataaggaaa aagttgcagc tcaagcctat actaaagaaa aaggtatcct
     4261 acctccatct ccagacgcag atgttaaagc aaatccaact ggagatagtg cagcagctat
     4321 ttacaatcgt gtgaaagggg aaaaacgaat tccactcgtt cgacttccat atatggttga
     4381 gcatacagtt gaggttaaaa acggtaattt gattattcct cataaggatc attaccataa
     4441 tattaaattt gcttggtttg atgatcacac atacaaagct ccaaatggct ataccttgga
     4501 agatttgttt gcgacgatta agtactacgt agaacaccct gacgaacgtc cacattctaa
     4561 tgatggatgg ggcaatgcca gtgagcatgt gttaggcaag aaagaccaca gtgaagatcc
     4621 aaataagaac ttcaaagcgg atgaagagcc agtagaggaa acacctgctg agccagaagt
     4681 ccctcaagta gagactgaaa aagtagaagc ccaactcaaa gaagcagaag ttttgcttgc
     4741 gaaagtaacg gattctagtc tgaaagccaa tgcaacagaa actctagctg gtttacgaaa
     4801 taatttgact cttcaaatta tggataacaa tagtatcatg gcagaagcag aaaaattact
     4861 tgcgttgtta aaaggaagta atccttcatc tgtaagtaag gaaaaaataa actaatgaaa
     4921 aatgaaagtc tcgataaaga ggctttcatt tttattatgt atatatgtaa aattcttgac
     4981 aagcaatatt aaaaagagta aactattaac tagttaatta accggtttat tactttatag
     5041 tgaatcaaat atacttaaga aaagaggaaa gaatgaaaat caataaaaaa tatctagctg
     5101 ggtcagtagc tacacttgtt ttaagtgtct gtgcttatga actaggtttg catcaagctc
     5161 aaactgtaaa agaaaataat cgtgtttcct atatagatgg aaaacaagcg acgcaaaaaa
     5221 cggagaattt gactcctgat gaggttagca agcgtgaagg aatcaatgcc gaacaaatcg
     5281 tcatcaagat aacagaccaa ggctatgtca cttcacatgg ggaccactat cattattaca
     5341 atggtaaggt tccttatgac gctatcatca gtgaagaatt gctgatgaag gatccaaact
     5401 accagctaaa ggacgaggat attatcagtg aaatcaaggg tggttatgtg attaaggtag
     5461 atggaaaata ctatgtttac cttaaggatg cagctcatgc ggataatgtc cgtacaaaag
     5521 aagaaatcaa tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg acttcaacaa
     5581 acgatggtgc ggtagccttt gcacgttcac aaggacgcta tactacagat gatggttata
     5641 tctttaatgc ttctgatatc attgaggaca cgggtgatgc ttatatcgtt cctcacggcg
     5701 accattacca ttacattcct aagaatgagt tatcagctag cgagttagct gctgcagaag
     5761 ccttcctatc tggtcgggga aatctgtcaa attcaagaac ctatcgccga caaaatagcg
     5821 ataacacttc aagaacaaac tggctacctt ctgtaagcaa tccaggaact acaaatacta
     5881 acacaagcaa caacagcaac actaacagtc aagcaagtca aagtaatgac attgatagtc
     5941 tcttgaaaca gctctacaaa ctgcctttga gtcaacgcca tgtagaatct gatggccttg
     6001 ttttcgaccc agcgcaaatc acaagtcgaa ccgccagagg tgtagctgtc cctcatggta
     6061 accattacca ctttatccct tatgaacaaa tgtctgaatt ggaaaaacga attgctcgta
     6121 ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaagacca gaacaaccaa
     6181 gtccacaatc gactccggaa cctagtccaa gtctgcaacc tgcaccaaat cctcaaccag
     6241 ctccaagcaa tccaattgat gagaaattgg tcaaagaagc tgttcgaaaa gtaggcgatg
     6301 gttatgtctt tgaggagaat ggagtttctc gttatatccc agccaaggat ctttcagcag
     6361 aaacagcagc aggcattgat agcaaactgg ccaagcagga aagtttatcg cataagctag
     6421 gagctaagaa aactgacctc ccatctagtg atagagaatt ttacaataag acttatgact
     6481 tactagcaag aattcaccaa gatttacttg ataataaagg tcgacaagtt gattttgagg
//
LOCUS       DAVKFH010000091         6516 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKFH010000091 DAVKFH010000000
VERSION     DAVKFH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.091"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..921
                     /locus_tag="VW006_001975"
     CDS             <1..921
                     /locus_tag="VW006_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9380766.1"
                     /translation="DEINKSLNYIFFSKNRLAIQNQNGKYYLLSRGKSVVPSRVSVGE
                     RNALALCYFFTEIIQQRELADAYSHEYFIVIDDPISSFDMENKVGITSYLKYCLTRFF
                     KGNSNTRVLLMTHDKQTIYDFDIFLKEIMESCKEEEGGQKSKYKKLELVSGKLQEFKT
                     STHDYTELLEIVFGYALGNSTPTSESFVGNAMRKILEAYGSFNYKKGIAELTTDPLIV
                     EKIDKEYRTYFENLMYRLVLNGESHFKDPVKTLSIDFFDTISDEERKKTARDLLVLLY
                     LLDDLHVLKHLEGVSNAENRLEQWKCEILE"
     gene            1094..2392
                     /gene="purB"
                     /locus_tag="VW006_001976"
     CDS             1094..2392
                     /gene="purB"
                     /locus_tag="VW006_001976"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9380767.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEINEIFNPIYYTKRVDDIFERLGLGD"
     gene            complement(2447..6385)
                     /gene="strH"
                     /locus_tag="VW006_001977"
     CDS             complement(2447..6385)
                     /gene="strH"
                     /locus_tag="VW006_001977"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9380768.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPRLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         1737 a         1232 c         1412 g         2135 t
ORIGIN      
        1 gatgaaatta ataaaagttt aaattacata tttttttcaa aaaatcggtt agctatacag
       61 aatcaaaacg gaaaatacta tctgttatca agaggtaagt ctgtggtccc cagtagggtt
      121 tccgtagggg aacgaaatgc gctggcatta tgttatttct ttactgagat aattcaacag
      181 agagagttag cagatgctta tagtcatgaa tattttattg ttattgacga tccaatttct
      241 agttttgata tggaaaataa agttggaata acctcgtatt taaaatattg tttaactaga
      301 ttttttaagg ggaatagtaa tactagagtt ttactaatga cccatgataa acaaacaata
      361 tatgactttg atatcttttt gaaagagata atggaatcct gtaaagaaga ggaaggtgga
      421 caaaaatcta aatacaagaa gttagaattg gtttctggta aattacaaga attcaaaact
      481 agtactcacg attatacaga actacttgaa atagtcttcg gttatgcatt gggaaatagt
      541 acacctactt cggaaagttt tgttgggaat gcaatgagaa aaattttaga agcctatggt
      601 agttttaatt ataagaaggg aattgccgag ctaacaacag atcctttaat tgttgaaaaa
      661 attgataaag aatacagaac ttattttgag aatcttatgt atagactggt tttaaacggt
      721 gaaagtcatt ttaaggatcc ggttaaaact ttgagtattg atttctttga taccatttct
      781 gatgaagaac gaaaaaaaac agctagagat ttactagttt tgctttatct tttagatgat
      841 ttacatgttt taaaacattt agagggtgta tcaaatgcgg aaaatagact tgaacaatgg
      901 aagtgtgaga ttttagaata agagaatcat accatttgac ttattgcaat ggttgaagtt
      961 tgcatgatag ctgattagag taatcaaagg attattactt cctaatttta ttttctacaa
     1021 accctaattt agcatctaat aatgcaaagt ttcctgagcc tcatagataa aaaagatttt
     1081 taaggagaaa aacatgatca accgttactc tcgccctgag atggcgaata tttggagtga
     1141 agaaaataaa taccgtgctt ggcttgaggt ggaaatcctc tctgacgagg catgggctga
     1201 gttgggggaa atccctaagg aagatgtggc tttgattcgc aagaaggcgg actttgacat
     1261 cgaccgtatt ttggaaattg agcaggagac gcgccacgat gtggtggctt tcacgcgtgc
     1321 ggtttctgag actcttggtg aagagcgcaa gtgggttcac tatgggttaa cttctaccga
     1381 cgtggtggat actgcctatg gttacctcta caagcaggcc aacgacatca tccgtcgtga
     1441 ccttgaaaac ttcactaata tcatcgctga caaggccaag gagcacaagt tcaccatcat
     1501 gatggggcgt actcatggtg tgcacgctga gccgacaact tttggtctta aattggcaac
     1561 ttggtatagc gaaatgaaac gcaatatcga gcgcttcgag catgcggctg ctggtgtaga
     1621 agctggtaag atttctggtg cggttgggaa ctttgccaat atcccaccat ttgtagagga
     1681 gtatgtctgc gataaacttg gcatccgtgc ccaagaaatc tctacacaag tccttcctcg
     1741 tgaccttcac gctgagtact ttgcggttct tgccagcatt gcgacttcaa tcgaacgtat
     1801 ggcgactgag attcgtggtc tacaaaaatc tgagcaacgc gaagtagaag agttctttgc
     1861 taaagggcaa aaagggtctt cagcaatgcc tcacaaacgc aacccaatcg gttctgaaaa
     1921 tatgactggt ctggcgcgtg tcattcgtgg tcacatgatt acggcttatg aaaacgtcgc
     1981 tctctggcac gaacgcgata tttctcactc atcagctgag cgtatcatca caccagatac
     2041 gaccattttg attgactaca tgctcaaccg ttttggaaat atcgtcaaga acttgacagt
     2101 cttcccagaa aatatgatcc gaaacatgaa ctcgactttt ggtcttatct ttagccaacg
     2161 ggctatgttg acattgattg aaaaaggcat gacccgtgag caagcctatg acttggtgca
     2221 accaaaaaca gcctactctt gggacaacca agtagacttt aaaccacttc ttgaggcaga
     2281 ttcagaagta acatcacgtc tcacacaaga agaaatcaat gaaatcttca acccaattta
     2341 ttacaccaaa cgagtggatg atatctttga acgtcttgga ctaggtgatt aattaaaaaa
     2401 taaacagcga gcttcaatct cgctgtttat tttttatcga aaagacttag tcttcttttc
     2461 ttttagtgag tccataggct gctagtgtgg acatgagtcc tgcgactact agtcctgcag
     2521 aatcgtgagt tcctgtttca ggaagttttt tctctgttac cacaggagct ggatcttgag
     2581 gaagagcttt gctttcctca gcaggagcag ttgatggagc tggttggctt gggatttcta
     2641 gtcttggttt ttcttcagca atagcggctt gtccgttttc atcgcctaca tgtgttacca
     2701 tagttccgac ttcgactatt tgagtaacgg cttcctgtgc tacgacacta tttacaagtg
     2761 ttttcacttc cttaccatcg gcagaagtgc tcacagagta gaagttgcta cgatgtccat
     2821 tgacgccctt agtaatgact tgtgtttttc ctttgagtaa gagtggattt tcacgagtca
     2881 ctgtggtaaa tggaatttct tctttttgga tatccagtct aggttttacc tcagtagttg
     2941 gtgcaagacc actttcatca cccttgtgag ttacaggagc gccaacttca accacttggt
     3001 ttataacttc tttggttacc tggctatcaa ggactgtttc tgttgttttt ccattttcag
     3061 tgagtacaga gatgtaatga gttcgttcac ctttgactcc tgctgtgata atattttcct
     3121 gaccagctgg gaggttagga ttttctttct tgataacttc aaatggaatt tcttcagttc
     3181 ttgtgatgag ttctggtctg gtttcaacat tggcagccac ttcattttca tccaggcttc
     3241 ctgaatgagt tgcagctggt ttgaggcctt gaagagcggc ttttaggttg gctacaagtg
     3301 tgtcaagctc agcttgttta ttacggttga ggttgtaatt tagagctgtt ttagctgcgt
     3361 caagggcctc aagactttct ttactatatc cttctaagtt tgtaggaatt ttagctaatt
     3421 cttcgcggag agcattataa ttagcacgga agtagtcttt gttgtggtct gcaaaggcag
     3481 tcatgagttc aaagatttcc tcttccttgt actcagcgct tggtctatct gcccagattg
     3541 aaagcatact tccgactgtt ggaagatcta cttcaggata tttggtagaa gctagttgat
     3601 tgaatggaac tttaccagaa ttttcaactg cttttgaaag agggtaagct tcatcttgtt
     3661 tgtgattgcc tataacataa taccagtcac cgttggtatt caagaattta tagcctttgc
     3721 ttgctaggta ttgaggtgat gcgaggttat atccccacca gcctttagac cagtaagaaa
     3781 tcaagacatc tttgtcaaac tgaacatcgt ccttgtcttc atagtagaag ccatcgttga
     3841 aggccattgg ttgaagccct ctttctttgg ccatagctgc gagggtgttg gcatattcgg
     3901 caaatttgcc atagagttga taccacttga ggtagtacca gccttgggca ctagtcgcat
     3961 cgttggcgta ttcgtcagta ccaaagttga aaatctttgt tttacctgca aagaagtcca
     4021 tgtatttacc gatgagggct tttacaaagt tcatcgcttc ttcgtttttc aagtccatag
     4081 ttgtttttga aactttatca aagtgggctt gaggattttt aatacctaat ttttccatgg
     4141 caaccagcat agcatccatg tgacctggac tgttaatagc tgggatgaga ccaatgtcct
     4201 tagatttagc gtattcaatt agctctgtca cttctgcctg tgttagtgca gtaccgtttg
     4261 gatcgtcgta gtaagcttta gttccttcga taatagcttt tttaacgtca tcactagcat
     4321 aggtttttcc gttggcagta atggtcatat cattgagtag aaagcgaagt ccgtcatttc
     4381 ctagaaggag atggacatca gaatatccga gctcactggc cttgtctacg atgcgtttga
     4441 gctggtccag agtaaagtat ttgcgtccag catcgattga gattaccttg tttttggcaa
     4501 gtttttcaac ctcacgttta gcttcttctt ctttttgagc ttcaggcgtg agggtcaagt
     4561 tgttgacagt ttcttgaagt ttagcaatgg cttgatcaat cgtatcttgt tgggcacggc
     4621 taaggttgct atcgagagag cgaatagctt tttcagcttc ttttacggcc gtgacgcttt
     4681 ctgcagtata acggttcagg tcttttggta cctcgttaag tgcttgctct gcagattcat
     4741 aatcagctgc gaagtattca gcgttggcat ttgcaaaatg acgcatgagt ttgaagaggc
     4801 gtgatggtga ataacgtgca gatggagtgt cagcccaagc agctaccata ccaccgatga
     4861 ttgggatatc agctccttct gtttttggta cagaagtgat tggtgtgttt ttaataccat
     4921 tgagcccctg atcgagattg taccagcctt ggccatcagc gtttcgtcca agaacgtagt
     4981 accaagcatc attggtatta aggatttggt gacctttttc agctagtagt ttagaagaag
     5041 cgacatcgta gcctccccaa ccaccagtcc acatagaaac gatgatgtct ttgtcaaaac
     5101 taccaaagct tgtgtcgcta ttgtagtaga taccgtcgtt aaaggccatt ggtttgagac
     5161 cgtgcgattt tacaatacga gcgaggtcat tggcgtaggc aataaatttt tcatagcctt
     5221 ttacagggta gccttcgttt ggatagtatt tatcagcttg aagcacactc caacctttag
     5281 catctgtcgc atcattggca tattcatcaa gtccgatgtt gaagatttca gtctttttcg
     5341 cgaaataagc agcatacttg tcgataaggg cttttgtaaa agcgacagct tgttcgttgt
     5401 caagatcgac agtacgggct gatttcttcc caaaatagct aaagttaggg ttttggattc
     5461 ccaattcttt catggcattg agaatcgcat ccatgtgtcc aggactattt actgtcggaa
     5521 tgagaccgat acctttatct ttggcatagt taatcagatc tgtcatttga ctttctgtta
     5581 agtgattgcc gtttggatcg ttgtaataat catttgtacc tttttcaatg gcgcgtttga
     5641 catcgtcact ggcataggtc ttgccgttag ctgtgatgct catatcgtcc aacatgaaac
     5701 ggagtccatc atttccgact aataggtgta aatcagtgta gccataatgt ttcgctttat
     5761 cgatgatttc cttgagctgt tctggtgaga aatatttacg tccagcatca atagaaacaa
     5821 ttttcttttt cgctagtttt tcatttacag ttgcagcacg ttcctttcct gtctctgttt
     5881 ccggtttgtc agcctctgct ttcgcttcat cttttttagc tggtttatcc ttgtcagtct
     5941 tgtctgtatt tgactcttta gaatcaacct ctttcgcttc ttccttttta gggctagctt
     6001 cttcttcctt tttattagca gtttcttttt cagcagaagt tggagttacc acctctgctt
     6061 tatcactagg agttgaacta acttcctctt gtggtttttc ttctgttttt ggaagactag
     6121 ctaccttatc agtagctgga gtttctgttt ctacagtttt tggagcttct ggttgaagct
     6181 ctgctttagg tgtttcctca gtccgatttt cggatgattg aggggaatca gaaaccgtat
     6241 ggatggtcgg ttggttttct gtagtagtag gagtaactcc atcggctgca acagtctgtg
     6301 cttggaaggc aaatccaatt agaacagaag ctgctcctac agcgtattta cgaatagaaa
     6361 aacgctgttg tttttcatgt ttcattgcaa aacctcctga ttgcattgtt atattgatag
     6421 cgattatata aatcaacgcc tttattttat ttcttatatt aacgagagtc aagaggagat
     6481 gacaaaaaac tataataagt ataaaaaaat ataaaa
//
LOCUS       DAVKFH010000092         5215 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKFH010000092 DAVKFH010000000
VERSION     DAVKFH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.092"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            145..672
                     /locus_tag="VW006_001978"
     CDS             145..672
                     /locus_tag="VW006_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9380769.1"
                     /translation="MEDYCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNT
                     RKAQGRHLEQEGNIKHALIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQ
                     TLMESLSDDDYDFAFDEALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKY
                     ECYSPLNNLELDDFE"
     gene            complement(838..909)
                     /locus_tag="VW006_001979"
     tRNA            complement(838..909)
                     /locus_tag="VW006_001979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(874..876),aa:Arg,seq:cct)
     gene            complement(951..1298)
                     /gene="rplS"
                     /locus_tag="VW006_001980"
     CDS             complement(951..1298)
                     /gene="rplS"
                     /locus_tag="VW006_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9380770.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1417..1746)
                     /gene="crcB"
                     /locus_tag="VW006_001981"
     CDS             complement(1417..1746)
                     /gene="crcB"
                     /locus_tag="VW006_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9380771.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1740..2114)
                     /gene="crcB"
                     /locus_tag="VW006_001982"
     CDS             complement(1740..2114)
                     /gene="crcB"
                     /locus_tag="VW006_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9380772.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(2111..2377)
                     /locus_tag="VW006_001983"
     CDS             complement(2111..2377)
                     /locus_tag="VW006_001983"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9380773.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2493..2936)
                     /locus_tag="VW006_001984"
     CDS             complement(2493..2936)
                     /locus_tag="VW006_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9380774.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            3040..3975
                     /locus_tag="VW006_001985"
     CDS             3040..3975
                     /locus_tag="VW006_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9380775.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            4029..4313
                     /locus_tag="VW006_001986"
     CDS             4029..4313
                     /locus_tag="VW006_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9380776.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4370..4588)
                     /locus_tag="VW006_001987"
     CDS             complement(4370..4588)
                     /locus_tag="VW006_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380777.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(4585..5159)
                     /locus_tag="VW006_001988"
                     /pseudo
     CDS             complement(4585..5159)
                     /locus_tag="VW006_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1679 a         1091 c          900 g         1545 t
ORIGIN      
        1 gcagaattaa ttacaagaat aattaataat gttaacattg acaatttaga tattccgttc
       61 gaatttaaac taacaaaaga agcacaaaat cttattatcg aacatagtga ctatatcaaa
      121 gcatactatg ataaagacat aactatggaa gattattgta aagaaaaaaa caatatctct
      181 tttaaagcaa cttttggtga tataaaatgg agtctcttaa ataaacaagc tcataggaat
      241 actgtatcag gagattttgg atgcttatct aacacacgaa aggctcaggg aagacatttg
      301 gaacaagaag gtaatattaa acatgcttta atatattaca tagaatcttt gataattact
      361 atttcaggat tagaaaacaa tttttcagcc actgattatc cagtatatta tcccgattcg
      421 atacctgact actcactaaa acatattcaa acattaatgg aatcattatc tgatgacgat
      481 tatgattttg cttttgatga agcattattt cgcttctcaa ttttgaatgc aaatcatttt
      541 ttatctaagg aagatattga ctatttaaga gttaatttac ctcgttccac tgctgaagaa
      601 ataaacaatt acttaaagaa atatgaatgt tatagtcctt taaataattt agaacttgac
      661 gattttgaat aaattgacta tacaaacatt tatatactcg atatagtctc aattttatct
      721 gatgattgcc caaatttttc aataataaaa cgcataatat tatggagaca atcccctata
      781 ttatgcgttc ttttaatatc aaagactttt tgacaaactt cttcgatatc taattactgc
      841 cccctgcagg aatcgaacct gcaactactc cttaggaggg agttgttata tccattgaac
      901 taagggagct agataaaaac tctgctgaaa agcagagttt tttagtcgaa ttaacgacgg
      961 atttctttga tacgagctgc cttaccttga agagcacgca agtagtacaa tttcgcacga
     1021 cgtactttac cgtaacgaac aacttcgatt ttttcaacac gtggagtgtg gattgggaag
     1081 atacgctcaa cacctacacc gttagagatt ttacgaactg tgtagttttc tgagattcca
     1141 gcacctttac gtgcgataac aacaccttca aaaatctgga tacgttcacg gttaccttcg
     1201 acaactttcg catgtacacg aacagtgtca ccaggacgga atgatgggat atctgtacga
     1261 agttgacctt cagtcaagct ttggattaat ggattcattt tattctccta tctttgtcaa
     1321 tcttgaggaa tcttcctcag cggataaact gtatttttgt gcgtccatta cacacaagat
     1381 atagtttacc aaatttctaa aaaaaagtaa agaaatttat agcaaaattc ctaaaaaaat
     1441 cgcaaccaaa ccacctatgt aagtcaaagt caggtaagaa taaaagacct tcttatcact
     1501 tagcagtctt tgaagttcgt cattcaaggt cgaaaaagtt gttaaaccgc cacaaaatcc
     1561 tgttgctaga atagcatata cttccttaga ctctacatga ttgtagaata ctccaattaa
     1621 aaaacaacct aaaagattag ctatgagcgt tccgagtggc aatttagaag cttgattata
     1681 gcgagaaaag aaataccgta cgagtgctcc caagccacag gcgattgcaa gatagacgat
     1741 taccatttct tcctccccaa atagtaagct aaaagcaggc ctccaccgat actcaaaagc
     1801 aaatacagta tcaaactaag ataacgccct gtatcaagca gcttcacagt atcaagcatt
     1861 aggctggaaa aagttgttaa acctccgcaa aaacccgtcc ccagtgctaa aatcaagccc
     1921 ttactagtcc ccttatagac cagatagccc ttgacaagat aaatcaagca gaaaattccc
     1981 agatagttga caaagagagt gccccaaggg aaatctggac tggctggtaa ccaagtggaa
     2041 actaggtagc ggacaagtcc gcccaacata gcagctagaa aaatccctag cggataaaat
     2101 tgttcttttt tcatttgata ttttgatcct gataatcacg cgaacgtttg agtatgtccg
     2161 aaaaagtcgc gacaatagtc tcctgatagc gcttgtcctc tacacgactt ctaacctttt
     2221 caaaaataac tgcttctctc ttagtatcta aaatcggttt ccctgaagcc ttcttataag
     2281 cgacaactcc ttcaactaag tgcattcgtt cttctaacag tttgacaatt tggtcgtcga
     2341 tttgatcgat ttcttgacga ataatatcta aatccataca gtctcctcct ttatttgaat
     2401 tattgtatca aaaagccatc aattaggcta gtaaaatccc aactctcgat aagaaaaatg
     2461 cactgattga ttacatcagc gcacttttat gcttatccta ctttagcagc caattcttct
     2521 gcgaattgtt ccaagcgttc aatatcttct tcctcggcag aaagatcaac tttaacacac
     2581 tctgaacctt tttctgctcc tgttgacaca aagacacggt caaagtcatc aacagcctta
     2641 caaaattcat cgtagaaggt atctcctgag ccgaccactc cgtagatttt gccattcaag
     2701 ttgagatctg ctaggtcttc gtagaagtcc atcatctcat ctggcaattc accatcacca
     2761 taagtatagg tcgcaacgat agcgatgtct gcttccaaga agtctgaagc gtcaacagtt
     2821 gtacattcat caacatcaac atccaagccc aagtcacgta atttgtctgc tacaatatct
     2881 gcaatttctt cggtattacc ggtcatactg gcaaatacaa tttttgctaa tgccatatcg
     2941 tcctcctcaa tttatctttc tccattatat cacatctttt tcattttaaa aataaaaatt
     3001 cttgtgctac aatgaaatga gaaagaattg aggttattta tggagatttg ccaacaaatt
     3061 ttagaaaaaa ttaaagaata cgacaccatt atcattcatc gtcatatgaa accagaccct
     3121 gatgccttgg gaagtcaggt gggattgaaa gccttgctag aacatcattt cccagaaaaa
     3181 accatcaaag ccgtcggttt tgatgaacca actcttactt ggatggctga gatggatctt
     3241 gttgaagata gagcctacca aggcgcactt gtcatcgtct gtgatacagc taatactgct
     3301 cgtatcgatg ataagcgcta tagtcaaggt gattttctca ttaagattga ccaccatcca
     3361 aatgatgatg tatacggtga cctgtcttgg gtcgatacta gttcaagtag cgctagcgag
     3421 atgattaccc tatttgccca aacaacccaa ctagccttgg cagatcgcga tgctgagttg
     3481 ctctttgcag gaattgtcgg tgatacaggt cgcttcctct acccttctac cactgcacgg
     3541 actcttcgcc tggctgctta tttgagagaa cataactttg actttgcggc tctcactcgc
     3601 aaaatggaca ctatgagcta caaaattgct aaactgcaag gctacatcta cgaccatctg
     3661 gaagtggatg aaaatggtgc tgctcgcgtt atcctgagtc agaaaatctt gaaacaatac
     3721 aatataaccg atgctgaaac tgcggccatt gtaggtgcac ctggacgcat tgacagagtg
     3781 agtctctggg gaatttttgt cgaacaggct gatggccact accgagttcg cttacgcagt
     3841 aaagtccatc ctatcaatga aattgccaag gagcatgatg gtggaggcca ccctctagca
     3901 agtggtgcta attcctatag cctagaagaa aacgaaatca tctaccaaaa gttaaaaaac
     3961 ttgcttaaaa actgataaaa tacttgccaa acttttcaga atctgataga ctagtatagt
     4021 aacaatctat ggctcgcaaa gagaccatgg cagaaaggaa atattgcaaa atgaaaaaag
     4081 atatccatcc agaatatcgc ccagttgtct tcatggacac aactactggt taccaattcc
     4141 ttagcggttc aacaaaacgc tctaacgaaa cagttgagtt cgaaggcgaa acttacccat
     4201 tgatccgtgt ggaaatttca tcagactcac acccattcta cactggacgt caaaagttca
     4261 ctcaagcaga tggacgcgtg gatcgtttca acaaaaaata cggtctcaaa taatgataag
     4321 agaacagttt tggctgttct tttttgtttc ttgaaatcaa ctgctgtttt catgttccag
     4381 actcatctgt aggttcgatt tccatgctac taggcaggaa ggaaatagct gtttcaacac
     4441 gtccataatg agctatacta ttgtcacgaa ccacactttc attgatggtc caagtggaat
     4501 tcattttctt aaaagcttct cggacttttt ccaaatcttt ggaggcaatg gcctgctcta
     4561 aggtttcaaa acgaggactt atactcatct gctttcaaaa agcattctag tccatctccg
     4621 attaccgatg gactttatca cctccttctc cagtccttgt atgacatctt gaagttgatt
     4681 catgacatct tccaaagttc gaaaggcttt attcttaaat ccacgtttac gaatctcttt
     4741 ccacacttgt tcaatgggtt catctctggt gtgtatggag gaataaaggt aaaatcaata
     4801 ttagtcggaa tatttaaggt acttgattta tgccatatag cattgtccat aacgagtaaa
     4861 aggataagct tgtgaaagct cttctaaaaa ggcgttcatc cacactcctt tttataaacc
     4921 tgaaataagg catcaattgt aacaaattct cctgcctctg tagccttcaa atgacgggca
     4981 agaaaggctt tctcttcctc aactgtcata tatgcatggt tacgaccacc acgtgtttct
     5041 tgaaggagag agtcgagtcc gaactcctca tattttttta cgtttcgcca aatcgttgtt
     5101 tgattacagt ctaaaagctc tataatctct ttataagatt tgcccatcag acgaaatata
     5161 gtagattgaa actagaatag tacacctcta cttctaaaac attgttagaa atcga
//
LOCUS       DAVKFH010000093         4679 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKFH010000093 DAVKFH010000000
VERSION     DAVKFH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.093"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(274..1002)
                     /locus_tag="VW006_001989"
     CDS             complement(274..1002)
                     /locus_tag="VW006_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9380778.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1160..2569
                     /locus_tag="VW006_001990"
     CDS             1160..2569
                     /locus_tag="VW006_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9380779.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2587..3882
                     /locus_tag="VW006_001991"
     CDS             2587..3882
                     /locus_tag="VW006_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9380780.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3887..4195
                     /locus_tag="VW006_001992"
     CDS             3887..4195
                     /locus_tag="VW006_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9380781.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4192..4500
                     /locus_tag="VW006_001993"
     CDS             4192..4500
                     /locus_tag="VW006_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9380782.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1582 a          799 c          862 g         1436 t
ORIGIN      
        1 agactcaaaa acttctcgtc agtgtaagaa tatttgaaac actatttaaa actaaataac
       61 tataagaagt agatcaggtg aacaatttca gtacctactc tataagctat acgaagatat
      121 ttttcaacat gatggataaa acgatcactc ttttaaagcc atcttattta gaaaagagaa
      181 accatttaag catagatggg accttcttgc cctgttttaa atttttctta aactagagtc
      241 tgtaaaagat tggcttcatt ttcacatttt ctcctaggag gaatgtcgta atgcaaaaga
      301 attaaattca aataaatcat agcgatgacg actaatcgag tactcaaacg gagttccgtt
      361 atccaaataa gccacttgtt ctacttcaaa taccggttcc gttggaagga gctgcaagta
      421 atgttgctca ttttctgaac tagaagaagc tcgtaagatt tttgtagcac tggcaatatg
      481 caatcctaac tttccttcaa tgtaattgta aatcgatttt tgtaaaatat cttcagtaat
      541 acctggaata acatcggtac tcatataagt ttgttccaga acataaggtt taccgtcaat
      601 aatacgcagg cggtaaatat tataaactgg actctgcaaa gcgacctgta gtttttctgc
      661 taaaaattca tttgcaaatt ctaatttgaa gtgaataatc ctcgagtcaa ttgtagaatg
      721 agcatcttca gatatttttg tcagtccccg gatatctctt tctggaacga ttaattttct
      781 ttttgagctg ccacgagaga gaacaaaggt tccctgccca cgttttctaa aaattaagcc
      841 ctcagaaact aacaagtcta aagctttttt gatcgtcatt cgactacagg caaactgttt
      901 agctaatgtc atttcatcag gtaattgttc gttcatggta tatttattac aaagaatctt
      961 ttcacgaatc tcattgtaaa taaataaata ttttgtactc atttaatctg gttctctcta
     1021 taatattcta atagtagtat aacaaagttc aagaaatgag gcaaacacaa gatatgtatc
     1081 aacaattttt aaaaatgtct agacaaattt taaaattatg ctatacttta tgtatagaat
     1141 aatagaaagg aggttgagca tgaaaaaaaa gttagtattt cctaatctgt tttggtgggg
     1201 agcagcttct agcggacctc agacagaagg tcaatatgga aaagtacatg aaaatgtgat
     1261 ggactactgg ttcaaaacgc atccagaaga ttttttcgat aatgtcggac ctcttgtagc
     1321 cagtaacttt tttcatactt acaccgaaga tttccacttg atgaaggaaa ttggagttaa
     1381 ttctttccgc acttccatcc aatggagtcg actcatcaag aatttagaga caggtgagcc
     1441 tgatccaaaa ggtattgctt tctacaatgc catcattgaa gaagctaaaa agaaccagat
     1501 ggatcttgtg atgaatttac atcattttga tttaccagtg gaacttcttc aaaaatacgg
     1561 tggttgggaa agcaaacatg tagtggagtt attcgtgaag tttgccaaga ctgctttcac
     1621 atgctttgga gataaggttc attactggac aactttcaat gagccaatgg tcattccaga
     1681 agcaggatac ttatatgctt tccattatcc aaatctaaaa ggaaagggaa aagaggccgt
     1741 acaagtcatc tataatctaa accttgctag tgcaaaagtg attcaactat atcgctcatt
     1801 agaacttgat ggaaagattg ggattatttt aaacttgaca cctgcttatc caagaagtaa
     1861 ttctccagaa gacttagaag caagtcgatt tacagatgac ttctttaaca aagtcttctt
     1921 gaatccagct gttaaaggaa ctttcccaga aagattggta aaacagctag agagagatgg
     1981 cgtgttatgg agtcataccg aaaaagagct tcaactgatg aaatcaaata cggttgattt
     2041 tcttggagta aactactacc atccaaaacg tgttcaagca caagcaaatc ctgaggaata
     2101 tcagacgccc tggatgccag accaatactt caaagagtat gaatggccgg agcgtcgcat
     2161 gaatccatat cgtggttggg aaatttttcc gaaatccatt tatgatattg ctatgattgt
     2221 gaaggaagaa tatggtaata tcccatggtt tatcagtgaa aacggaatgg gtgttgaaaa
     2281 cgaagcacgg tttatcgatg aaaatggagt tatcgatgac gtgtatcgta ttgaatttta
     2341 tgaagaacat ttaagatggc tacataaagc cattgaagag ggaagtcact gttttggata
     2401 ccacgcttgg accgcatttg attgctggtc ttggaataat gcatataaga atcgttacgg
     2461 atttatctcc gttgatttag aaacgcaaaa gagaaccatc aagagctcag gaagatggta
     2521 tcgcaaagta agtgacaata acggttttga agtagaaatt gaggagtaaa gagaggagat
     2581 attcatatga aaaactcaaa atttatagac caatttgcca cctttgctgg taaactaggg
     2641 aaccaaattc atttaaaaac cctaagagat gcattcgtaa cagtaatgcc attatatatt
     2701 ttggcaggtt taatcgttct tttgaacaac acggtattta agtggatttt ccaaggggat
     2761 acattaacaa gattccaata ttggggaata acaattgcaa acggtacttt aagtatttca
     2821 ggtatgatta ttgctgtaat ggttggttat ttcttagcta aaaacagaga tttcgaaaac
     2881 ccgttagcag catcaatgct atcattagtt tctttaattg tgatgatgcc aaatacagtt
     2941 tctgtagttc ctgacggagc aaaagatgct gtaaacattt caggtgttct ttcattcaac
     3001 aacacaggta caggcgcaat gttcgccggg gttatcgtag cgattattgc aacagaatta
     3061 ttcattgaat tatcaaatgt taaagcttta caaatgaacc ttggtgaaaa tattccacca
     3121 gctgttaata gatcatttag cgtattactt ccagtcatga ccgtcatctc cttatttggg
     3181 gttgtttcag cattattatt caatataact ggaatgaact taatctcaat cattacaatc
     3241 tttattcaag aaccaattcg tcatattggt acaagcttaa tcggggtcat tattatttac
     3301 tctttaggaa atatgttatg gctatttggt attcaccaag cagttattta cagtgccatc
     3361 ctagaaccat tactattaat taacattact gaaaacatca ctgcagcaaa taatggacaa
     3421 gccattccac acatcatcaa cctatcacaa atacaaacat tcgctttaat gggtggtagt
     3481 ggatctacat tatgtttatt aatagcaaca ttcttagtga gtcgcaatgc tgtctctaaa
     3541 aacgtggcta aattatcttt tggacctggt atcttcaata tcaatgaacc agtattattc
     3601 ggttacccaa tcgtttataa catttcatta gctattccat ttatcacagt tccagtcctt
     3661 ggtattttaa tcagctactt agcaacagtt acaggattca tgagtcctgc atttatacaa
     3721 gttccttgga ctacaccagt attcttaaat gcatggttag caacagcagg ggacgtgaga
     3781 gcagttctag ttcaattcat catctttgca cttggagttc ttctatacat tccatttatc
     3841 aaagttaatg acaaagttgt tgaacaagaa atggaaggtt aagattatga aacaaatttt
     3901 attagtatgt aatgcgggaa tgtcgacaag tatgcttgtt aaaaaaatgc aacaaagtgc
     3961 gactgaacgt gggattgaaa tctctatcca agcaaaatca atgacagaag ctaaaaagaa
     4021 tatccatgaa gcagatgtca ttctaattgg gccacaaatt cgttatgagt tacttgctgt
     4081 gaaagaaatt gcaggaaata tccctgtaga taccattgat atgcgggatt acggaatgat
     4141 gaatggagca aaagttctgg aacaagcact tgcatggata ggagaaataa gatgacagaa
     4201 gaaatggaat atatctgttt tcagctcatt gccaatagtg gagcagccaa atcatccttt
     4261 attgaagcca tccaactagc taaggcagga aatttaaaag aagcgaaaat taaagttgaa
     4321 gaagcggaag actccttagt agaagcgcac aagattcact ctaatctcat acaaaaagaa
     4381 gctactggag aaaaaatagg attctccttg ctatttatgc atgcagaaga ccaaatggct
     4441 tcaacagaga ttattcagtt gctatctaaa gaattcatcg atctctatca aaataaatag
     4501 gaacactgtc attcccatct gtctttatta atgacgacta cattgtagga acctccaaaa
     4561 gttggacaaa atgattgaaa ggattggatc ctgcattaca caagatccaa tccttttctt
     4621 ttactctagt ttttataata tgttgaatct agaatagtcc actgtagttt ctaaaaaat
//
LOCUS       DAVKFH010000094         4644 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKFH010000094 DAVKFH010000000
VERSION     DAVKFH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.094"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..648)
                     /locus_tag="VW006_001994"
     CDS             complement(<1..648)
                     /locus_tag="VW006_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9380783.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKF"
     gene            complement(936..1898)
                     /gene="nrdF"
                     /locus_tag="VW006_001995"
     CDS             complement(936..1898)
                     /gene="nrdF"
                     /locus_tag="VW006_001995"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9380784.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(2086..4245)
                     /gene="nrdE"
                     /locus_tag="VW006_001996"
     CDS             complement(2086..4245)
                     /gene="nrdE"
                     /locus_tag="VW006_001996"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9380785.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4326..4544)
                     /gene="nrdH"
                     /locus_tag="VW006_001997"
     CDS             complement(4326..4544)
                     /gene="nrdH"
                     /locus_tag="VW006_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9380786.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
BASE COUNT         1351 a          858 c          952 g         1483 t
ORIGIN      
        1 aaacttattg aacttactat gatctgcgag taaatatttt ttattagaat tatttaaagc
       61 gatgcgttga gcctctccct cttcctcgct aaaagtagct agagctccgt tttgaatacc
      121 attacagcta acgaaagctt tagaaaattg gagattagag agattttgta gggtcaatgt
      181 gccaacaaaa gcacctgtaa tatcgcgata atttccacct atcaaaatca aatctgttaa
      241 ttttcgttcg cttaaaatca gaaaaacagg tagactgttg gttacgacgc ggatattgtc
      301 aataggcaac tcacgcgcaa aaaactctaa tgttgttcct ggtccaatga aaatagtttc
      361 tctttcttct actagactgc ctgcaaaatg ggctatttct tgtttttctg ccgtttggag
      421 ggcttgtttt tcaatatttg atcgctcatt agtcaaaagg gagttggttc gaagtttttc
      481 agctccacca tgcacacgaa tcagcaaatc tttatcagct aattcctgta aatagcgcct
      541 tgcagtcata tctgaaacgg ctatttcgtc cataatctgt ttaactgtta tggttccttt
      601 actatttact atctctaaaa ttttggttaa tttttcttgt ttgagcatat tatcacctcg
      661 tttcctacta ctatcttacc ataaacaaac tcatcattca aatacaaaaa caacaaaatg
      721 aaacaaaaac aaaaatatcg aagtttgttt tcaaaacttt cgatattttt gttgggttat
      781 tattttgttg tttctagttt actttttgat ggttaagagt ggtggagaat tatactcaat
      841 gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgt acttgagtac ggcaaggcga
      901 agctgacgtg gtttgaattt gattttcgaa gagtattagt ccaaaccgta gttgtagtca
      961 tcatcttgca tggcttcaac ttcgccaaga aggtaaccat ttccgacttg agagaagaag
     1021 tcatggttgg aagttcctgt tgaaataccg ttcataacga ttgggttgac atcttcagct
     1081 gaatctggga aaagtggatc ttgtcccatg ttcatgagag ctttattggc attgtagcga
     1141 aggaaggttt taacctcttc agtccaacca acaccgtcat aaagactctc tgtgtagcct
     1201 tcttcatttt cataaagagt atagagtagg tcgtacatcc attctttgag tttttcttgc
     1261 tcttcttcag gtaattcatt gaaaccaagt tggaatttgt aaccaatgta ggttccgtga
     1321 acagactcgt cacgaataat caatttaatg atttctgcaa cgttggcaag tttgttgtta
     1381 ccgagatagt agaggggagt gaagaaacca gagtagaaga ggaaggtttc gaggaagacg
     1441 ctggcaactt tcttttcaag tgggctgccg tttaggtaga tttcgttgac aatctcagcc
     1501 ttcttttgta ggtaaggatt ggtattggtc cattcgaaaa tttcttcaat ctcagcctta
     1561 gtattcaagg tagaaaagat tgatgagtaa gatttagcgt ggacagattc cataaattgg
     1621 atgttattga aaacagcttc ctcatgtggt gtacggatgt ctgcgcgaag ggcttgaacc
     1681 ccagtttcag attgcatagt gtcaagaagg gttaaaccac caaaaacttt tccgaccaag
     1741 tctttctctt tgttagatag ctttctccag tcatccaagt cgtttgataa gggaatacgt
     1801 gtatcgagcc aaaattgctc cgtcagtttt tcccaagttg atttgtcgat gacatcttcg
     1861 atggcattcc agttaatggc tttgtagtaa gtttccattt aaaatctctt tctgtgttta
     1921 gtattgcgaa ctcacaatta tttctacttt accataattc tataggagta tcgcacaaaa
     1981 agtcggaagc ccgactttta aaatgttaca taaattatgt tatgacatag tagatttgat
     2041 tttatcagtg ctgcttaggg aaaaatagtg tttctatgct agaaactaaa tcacacagct
     2101 ttcacattgg ttggcgccga cttctccacc gtcatctgta aaggtacgga cgtagtagat
     2161 agacttgatt cccttgttaa aggcatagtt acgaaggatg gacaagtcac gtgtcgtttg
     2221 tttattttcc ctcttccatt cgtaaaggcc ttttggaatg tcactacgca tgaagagggt
     2281 gagtgaaagt ccttgatcca cgtgttcagt cgcagcagcg taaacatcga tgactttacg
     2341 catatccata tcgtaggcag aagtgtagta aggaatggtt tctgtagaca agccagcagc
     2401 agggtagtag attttaccaa ttttcttttc ctgacgttct tcaatacgtt gcgtaatcgg
     2461 gtggatagaa gcagaaacat cgttgatgta gctgatagaa ccatttggcg ctacagcaag
     2521 gcgattttgg tggtaaagac catcctcttg aaccttgtcg cgaagttcag tccaatcagc
     2581 aacaccaggg ataaaaacat ttttgaagag ttctttaaca cggtctgatg ttggaacaaa
     2641 ttcaccagtt acatacttgt caaagtaact tccgttagca tagtctgatt tttcaaagtt
     2701 gtggaaggta ataccacgtt cacgcgcaat attgtttgac tctaccaagg tccagtagtt
     2761 cataagcata aagtagatgc ttgtaaattc aacagactca ggtgaaccat attcaatgag
     2821 ttgttgggca aggtagctgt gcagtcccat ggcaccgaga ccaaaggtgt gggcttggct
     2881 atttccatgg tcaatcgttg gtacagctac gatatgtgaa ctatctgtaa cgaaagtaag
     2941 ggcacgaacc atagcacgga tagaacgacc aaaatcaggt gaagtcatca tgttaaccac
     3001 gttggttgaa cccaggttac atgaaacatc tgttcccatt tgaaggaatt cttgagcatc
     3061 gttgatcaag cttggttctt gaacttgaag aatctcagaa cacaagttac tcatgataat
     3121 ctttccatcg acaggatttg cacggttagc cgtatctatg ttgactacat aaggatagcc
     3181 agactcttgt tgcaatttag agatttcagt ttccaaatcc cgcgccttga tttttgtctt
     3241 gcgaatattt ggatttgcga ccaattcatc gtatttttca gtaatgtcga tgtaattgaa
     3301 tggcacaccg tattcttttt ctacagagta agggctgaag aggtacattt cttcattttt
     3361 acgagccaat tcgtagaatt tatcaggtac tacaacacca agtgatagag tcttgacacg
     3421 tactttttca tcagcgtttt ctttcttagt tgaaaggaaa gcgatgatat ctgggtgaaa
     3481 gacgttgagg tagacaacac cggcaccttg acgttgcccc aattggttgg agtaagagaa
     3541 gctgtcttca aaaagcttca taacaggaac gacacctgaa gcagctcctt catagccttt
     3601 gataggtgca ccagcttcac gaaggttgct gagggtaatt cccacaccac caccaatacg
     3661 tgaaagttga agagctgagt tgatagaacg tccgatagag ttcatatcat ccgtcacttg
     3721 gattaggaaa caagatacca actccccacg acgagcacgt ccagcattca agaaggaagg
     3781 agtagcaggt tggtagcgtt ggtggatgat ttcattggca atatcgattg caacagcttc
     3841 attcccatca gcgaaataaa gggcgttgaa gaagacacgg tcttccatat tttcaagata
     3901 gtattcaccg tcattagtct ttaaggcata ttgattgtaa aatttataag ctgccatgaa
     3961 tgacttgaat tggaagtttt ggtctttgat aaattgagcc aattcttcca agaactctgg
     4021 acggtatttc ttgataaagg ctgtttcgat gtagttgtgt tcaatgaggt aattgatttt
     4081 gtctttgatt gaatcaaaaa ccatagtgtt tggaactaca ttttctttaa agaaagcatc
     4141 caaggcttcc ttatctttat gaagcatgat ttgtccatta acaggacggt taatttcgtt
     4201 attaagacgg aagtaagtca cgtcttcaag atgttttaat cccataaaat ttcccttatc
     4261 taattacaaa agaaaggctt ctaagttagc cctaaaagca gtttcttctg gatgatgtac
     4321 taagattatg ctaattgttt cagtttttct ggttggaaac ctgaaaagac ttcagttggt
     4381 gtttggataa caggagctgc gctaaaaccg agctctttaa cttgatcgac gtactcaggt
     4441 tgctcatcaa gattgatttc acgataagag acattattac tgtccaagaa acgcttggtc
     4501 attttacatt ggacacaatt gtttttagaa taaacggtta ccattgtgta actcctcttc
     4561 aaaatttaat actatcttag tatatcagaa aataaaattt tgtcaatgat ttaaactaaa
     4621 tatagtggtg ttcttttgtt gttt
//
LOCUS       DAVKFH010000095         4333 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKFH010000095 DAVKFH010000000
VERSION     DAVKFH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.095"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            42..1067
                     /locus_tag="VW006_001998"
     CDS             42..1067
                     /locus_tag="VW006_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9380787.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1057..2064
                     /locus_tag="VW006_001999"
     CDS             1057..2064
                     /locus_tag="VW006_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9380788.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2064..3071
                     /locus_tag="VW006_002000"
     CDS             2064..3071
                     /locus_tag="VW006_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9380789.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3071..3865
                     /locus_tag="VW006_002001"
     CDS             3071..3865
                     /locus_tag="VW006_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380790.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT         1569 a          636 c          735 g         1393 t
ORIGIN      
        1 ttatcgatac aatagacttg aaatatgttt aaggagtttt tatgaaaaac aaattttttc
       61 taatagctat tttagctatg tgtatagttt ttagcgcttg ttcttctaat tctgttaaaa
      121 atgaagaaaa tacttctaaa gagcatgcgc ctgataaaat agttttagat catgctttcg
      181 gtcaaactat attagataaa aaacctgaaa gagttgcaac tattgcttgg ggaaatcatg
      241 atgtagcatt agctttagga atagttcctg ttggattttc aaaagcaaat tacggtgtaa
      301 gtgctgataa aggagtttta ccatggacag aagaaaaaat caaagaacta aatggtaaag
      361 ctaacctatt tgacgatttg gatggactta actttgaagc aatatcaaat tctaaaccag
      421 atgttatctt agcaggttat tctggtataa ctaaagaaga ttatgacact ctatcaaaaa
      481 ttgctcctgt agcagcatac aaatctaaac cttggcaaac tttatggaga gatatgatta
      541 aaattgattc aaaagcctta ggtatggaaa aagaaggtga tgagttaatc aaaaatactg
      601 aagctcgtat atccaaagaa ttagaaaaac atccagaaat caaaggaaaa atcaaaggaa
      661 aaaaagtatt atttactatg attaatgctg cagatacatc aaaattctgg atttatacta
      721 gcaaagatcc aagagcaaat tatttaacag atttaggtct agttttccct gaatcattaa
      781 aagaatttga gagtgaagat agttttgcaa aggaaatttc tgcagaagaa gcaaataaga
      841 taaatgatgc tgatgtaatc ataacttatg gtgatgataa aactcttgaa gctttacaaa
      901 aagatcctct tttaggtaaa ataaatgcaa ttaaaaatgg tgccgttgct gtaattccag
      961 ataatacacc gttagcagcc tcatgcactc caacaccact ttcaataaac tatactattg
     1021 aagaatacct aaatctttta ggaaatgcat gcaaaaatgc gaaataaaaa acaaataaac
     1081 ctaggcataa tttttgtaat ctgcctaggt cttcttatta caatattttt gtcattaaag
     1141 cttggaacaa aagaaattaa tatcagagat tttttagcag cttttggaat gggcaataca
     1201 aatgatgatt ttattaaatc aattatatat aaaagaatac ctagaactat ttttgcaatt
     1261 ttagcaggtt ctagtcttgc cataagcggt gtattgatgc aatcagttac tagaaaccca
     1321 atagctgatc caggtatact cggtataaac acaggagcaa gtcttagtgt agtaattggt
     1381 ctttcttttt taggaatttc atcaagcata agccatataa gttttgcaat cattggtggc
     1441 ttagtaagtg caatttttgt atacgcgatt gctgtaagcg gaaaagcagg ccttacccct
     1501 ataaaacttg ccttatcagg aacttgtgtt agtatggctt taagcagttt tgtaagtttt
     1561 ttaattttac cgaataataa cgtcttagac aaatttagat tttggcaaat aggtagcctt
     1621 ggagcagcta cattatcttc tatatctaca ctactacctt ttataatttt aggtcacttg
     1681 atagctatat ttatttcatc agatttaaac gctttagcta tgggtgatga aatggctgtt
     1741 ggtcttggag ttaatgttaa taggataaga tcacttgcaa taattgcaag tgtgctttta
     1801 tgttcaagta ttactgcaat tggtggacct attggcttcg taggtcttat agttcctcac
     1861 ttttgtggct tatttataag caaagatata cgcacaatga ccatttcttc atcttttata
     1921 ggtgcagagc tcttgcttat atgtgatata atcggccgta tgttaggtaa accaggtgaa
     1981 attgaagtag ggataattac tgcaataatc gggggtccag tacttattta tgtaactatg
     2041 aaaaatagag gggttaataa ctaatgcaaa atttaattat aggtattcaa aaaagaaaaa
     2101 atagaataac actattttcc tcactatttt tattaataat aatcagtcta tcatttttca
     2161 ttttacttat cggagatgaa agttattctt tttcaacttt gattaaagtc ttaaatagtg
     2221 aaactgttcc tggagctagt ttttcgatta tggaaattag attaccaaaa ttattagcag
     2281 gaattatagc tggctggtct tttggattgg caggatttat ctttcaaact atgttaagaa
     2341 atcctcttgc aagtcctgat ataatcggtg tcacaagttc ttcatctatt gcagcggtct
     2401 tttgcatatt ggtattaaaa acaaatagtt taactactgg aattatttca ataacttgtg
     2461 gactaacatc atctttaata ttatttttac tagctaaaaa agatggtttt tcagcagcaa
     2521 gactgataat attaggtatt ggttttcaag ctgtcacaag agcaggcacc tcatttttat
     2581 tgttgaaagt agcaagatat gaattacaag aagttatgag atggctcagt ggctctttat
     2641 cttttacaaa gttagatgac atacctcttg ttctaatagt aagtattatt gctactatat
     2701 tagttttatt ttttaataaa agactagaaa ttattgaact tggtgaagaa atagcaatcg
     2761 gacttggagc aaatcccgag ctttcaaggc ttgttttaat tttttgcgct gtatctttaa
     2821 ctgctttttc tacttcaatt acaggaccaa tagcttgtat atctttttta gctggtccca
     2881 tagccttaaa tattggcaag aaaagaagcc caatattagc tggattggtt ggaattttac
     2941 tagttttgtt atcagacata ttctctcaaa atattttacc agctagatat ccagtaggtg
     3001 ttgtaactgg cttgttaggt tcaccatact taatatactt actaataaaa atgaacagga
     3061 ggaatatata atgaaaggtc tgtggtcaaa taatcttacc tgcggttatg atgaaaaaat
     3121 aatcttggaa aatataaata taaaaatacc tgaagaaaaa atatcagtta ttattgggtc
     3181 aaatggttgt gggaaatcaa cactcattaa aaccttgtct cgacttataa agccattaga
     3241 gggagaagta ttgcttgata ataaatcaat taattcttat aaagaaaaag atttagcaaa
     3301 acacatagct atattacctc aatctccaat aatccctgaa tcaataacag tagctgatct
     3361 tgtaagccgt ggtcgtttcc cctacagaaa gccttttaag agtcttggaa aagatgacct
     3421 tgaaataata aacagatcaa tggttaaggc caatgttgaa gatctagcaa ataacctagt
     3481 tgaagaactt tctgggggtc aaaggcaaag agtatggata gctctagccc tagcccaaga
     3541 tacaagtatc ctacttttag atgagccaac tacttacttg gatatctcat atcaaataga
     3601 actattagac ctcttgactg atctaaacca aaaatataag acaaccattt gcatgatttt
     3661 gcacgatata aatctaacag caagatacgc tgattaccta tttgcaatta aagaaggtaa
     3721 acttgttgca gagggaaagc ctgaagatat actaaatgat aaactagtta aagatatctt
     3781 taatcttgaa gcaaaaatta tacgtgaccc tatttccaat tcgcctctaa tgattcctat
     3841 tggcaagcac catgttaact cttaataatt gaatattgga attcatttaa aagtcgcaaa
     3901 ttttgaactg accctcaaaa gttaggccaa aaaactaact aaaggaggtc agttttttaa
     3961 tggcaaatac agcacagaat ttaagataaa attagtaaaa gaatatcatg aatgaaaaat
     4021 aggatataga gaataacagc aatattaaaa agatcaggac tgataataaa caaaaagaaa
     4081 gtacaacgac tagttcaaaa gctaaaactt caagtaaaaa gttattcaag aaaatctaga
     4141 aaatactcat cctacaaagg acaggtagga aaaatatcag acaacaaaat aaaaagaaat
     4201 atttattacc gaaaaaaagg ggaaatcgag ttaagtacca acaggctcat ctattccaaa
     4261 aagatattta ctcgatggaa aaactcctag aattagtgct acaaatattg acaatggtat
     4321 cttaggttac tac
//
LOCUS       DAVKFH010000096         4320 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKFH010000096 DAVKFH010000000
VERSION     DAVKFH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.096"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..168
                     /locus_tag="VW006_002002"
                     /pseudo
     CDS             1..168
                     /locus_tag="VW006_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            335..535
                     /locus_tag="VW006_002003"
     CDS             335..535
                     /locus_tag="VW006_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380791.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            577..982
                     /locus_tag="VW006_002004"
                     /pseudo
     CDS             577..982
                     /locus_tag="VW006_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1213..1920
                     /gene="ccdA2"
                     /locus_tag="VW006_002005"
     CDS             1213..1920
                     /gene="ccdA2"
                     /locus_tag="VW006_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9380792.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1931..2488
                     /gene="sdbB"
                     /locus_tag="VW006_002006"
     CDS             1931..2488
                     /gene="sdbB"
                     /locus_tag="VW006_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9380793.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2862..4253
                     /locus_tag="VW006_002007"
     CDS             2862..4253
                     /locus_tag="VW006_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9380794.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT         1266 a          783 c          912 g         1359 t
ORIGIN      
        1 aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac aaatggtgaa
       61 ttaatcgctc caatgactta cgaagagatg atgacgagcg acttttttga agtatggttt
      121 cagaagtttc tcttaccaac attaaccaca ccatcggtta ttatagtaaa atgaaataag
      181 aacaggacaa atcgatcagg acagtaaaat cgaattctaa caatgtttta gaagtccata
      241 tgtactattc tagtttcaat ataccataac tgttcctagt caacctgcaa gccctgtccc
      301 agctccaagc tcagctccga ctcaagatcc tagagtggta gcaagcgctt cggctagctc
      361 aacgagcaca caagctcaag agcaagttga caagtctgaa cttcgtgcct tgagtcaaga
      421 gttagaccaa cgcttgaaag ctttggcaac agtatctgat ccgaaaatcg atgcaaccaa
      481 ggctgtcctc ctagatgctc aaaaagctcc ggaagatagt gccttgacag agtaaggtct
      541 gcatactgca gtagagtctg tgaaggctgg gctgccttgg actctctcaa agatgtgaaa
      601 gcaaatgcta gcgacagcaa gcctacacag gacaagaagg atgcaaaaca aggaacggaa
      661 gatagtaagg attcagataa gatgactgaa acaaactcag ttccagcagg agtgattgtg
      721 gtcagtctac ttgccctcct aggcgtgatt gccttctggc taattcgccg taagaaagag
      781 tcagaaatcc agcaattaag cacggaattg accaaggttc taggacagct agatgcagaa
      841 aaaagcggat aaaaaagtcc ttgccaaagc ccaaaacctt ctccaagaaa cccttgattt
      901 cgtgaaagaa gaaaatggct cagcagagac agaaactaaa ctagtagagg agcttaaagc
      961 aatccttgac aaactcaagt aaaacatgat ttatgaaatg tcagacctaa aagacatttt
     1021 cgttcacaaa agcagcgacg agtaatcctt gtcgttgctt tttggctatt ttgggggata
     1081 agatccgcct tgactagcaa aagaaaagat tggtccaata gtgcccgata cgattttgcg
     1141 tagcagaaag cttacaaata aaattgaata tagtaaaata ggattagaat tattaagaaa
     1201 gttggttctt tattggaaac gatagtattt ttaatctctg tttttctagc aggtgtttta
     1261 tccttttttt ctccttgtat ttttcctctt ctgccagtct atgctgggat tttattggat
     1321 gatcaagaaa gtgcaaaaag cttttctttg tttgggagaa aggttctctg gtcaggcttg
     1381 attcgaacac tttgctttat cgctggtatc tctctcattt tctttattct aggctttggt
     1441 gctggttact ttggtcatat tctctatgca aattggtttc gatatggcat gggagctatt
     1501 attatcattt tgggtcttca ccagatggaa atttttcatt tgaagaaatt agaagttcaa
     1561 aaaagtttta cctttaaaaa atcagactct aatcgttatt ggtcagcttt tttacttggt
     1621 attaccttta gctttggttg gacaccttgt attggtccag ttttaagttc tgttttagca
     1681 cttgcggctt ctggaggcaa tggcgcttgg caaggagcga tttatactct catttacact
     1741 ctgggcatgg cccttccttt cttggtattg gcactagctt caggtctagt catgccatat
     1801 tttagtaaaa tcaagcgtca tatgatgcta ctaaagaaaa ttggtggttt cctcattgtt
     1861 ttaatgggaa ttttgttact attaggacaa gtaaatgttc tagctggaat ttttgaataa
     1921 aggaggacaa atgaaaaaag taatgtttgc tggcttaagt ctcttgtcat tagttgtatt
     1981 gatggcctgt ggtgaggaag aaactaaaaa gactcaagca gcacaacagc caaaacaaca
     2041 aacgactgta caacaaattg ctgttggaaa agatgctcca aacttcacat tgcaatccat
     2101 ggatggcaaa gaagttaagt tatctgattt taagggtaaa aaggtttact tgaagttttg
     2161 ggcttcatgg tgtggtccat gcaagaaaag tatgccagag ttgatggaac tagcggcgaa
     2221 accagatcgt gatttcgaaa ttcttactgt cattgcacca ggaattcaag gtgaaaaaat
     2281 tgttgagcaa ttcccacaat ggttccagga acaaggatat aaggatatcc cagttcttta
     2341 tgataccaaa gcaaccacct tccaagctta tcaaattcga agcattccta cagaatattt
     2401 aattgatagc caaggaaaga ttggaaagat tcaatttggt gctatcagta atgcggatgc
     2461 agaagcagca tttaaagaaa tgaactagaa tcgataaaat ctgattacaa gatgtagtca
     2521 gattttggta acttctaaaa ctaacttcca gtttcccaca acctagcttt tagtatgaga
     2581 aaaacgcgtg acatcagtgg aaatccgtct tagactaact tccactggtt ttctttttct
     2641 tgatatataa gggttcaaaa gcgaaaagac tcagaaaaag tgcacgacaa atagccctaa
     2701 aacagagtcg tcttagagta acttccagtt ttgggagaga gatggaagtt actttgagaa
     2761 gttacggtag tcagattttt ttagaaaaac attttataaa tattcacata tctcctatat
     2821 ttatggtaaa atagaatcat cagtttattt tggagtcaaa gatgaatata tttagaacaa
     2881 agaatgttag tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt
     2941 tgctgggtat cggagccatg gtagggacag gcgtctttac aatcacaggt actgcagctg
     3001 caacacttgc tggcccagcc ctagtgattt caatcgttat ttctgccttg tgtgtgggat
     3061 tatcagccct cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata
     3121 gttacctcta tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga
     3181 tggagttcat gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct
     3241 tgctctctca atacgggata gcctttcctc aggctttaaa tggtaccttt aatcctcaag
     3301 cagggacatt tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt
     3361 tactaaatgc caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg
     3421 ctttagctct ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt
     3481 ttgctcctta cggttttgga caaatctatg gagctagcac tggtattatg gctggtgcgt
     3541 ccttgatgtt cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca
     3601 agactcctca aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc
     3661 tctatgcctt ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg
     3721 atgatgccgt tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt
     3781 cattagtggc tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc
     3841 gtatgattta cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga
     3901 agactagcaa gataccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag
     3961 caggaatgtt cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact
     4021 tgatcatgtt ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg
     4081 gggaatttaa aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat
     4141 ccttcatgtt acagtataat atgaatacct ggattgcttt cctagttgcg ttgttagtag
     4201 gaagtattat ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa
     4261 gtctattgag ttgttgacac tcagcagatt tttatatgtg aaagaaattt caaatttttt
//
LOCUS       DAVKFH010000097         4136 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKFH010000097 DAVKFH010000000
VERSION     DAVKFH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.097"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            720..1712
                     /locus_tag="VW006_002008"
     CDS             720..1712
                     /locus_tag="VW006_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9380795.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VW006_002009"
     CDS             1714..2532
                     /locus_tag="VW006_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9380796.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VW006_002010"
     CDS             2534..3319
                     /locus_tag="VW006_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9380797.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="VW006_002011"
     CDS             3417..3635
                     /locus_tag="VW006_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9380798.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3781
                     /locus_tag="VW006_002012"
     CDS             3620..3781
                     /locus_tag="VW006_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9380799.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
BASE COUNT         1153 a          703 c          896 g         1384 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat taatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta caaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cggtgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tatttcatga
      541 gagaaatcct aatttcacaa tctataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttctgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aacattttgt gaagattgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg agcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca aaagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctatc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttcg tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttaatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgtt gaggttttga
     3421 aggatttgcc agaatggttt ggaatcccag aaagcacaca agcctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaaggttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagg ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt tctcagctat ggaatccgta
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctattctcag
     3841 agtgccatac tgtaagtgta atcgccgatt tagctcagtt ggtagagcaa ggcactcgta
     3901 aagcctaggt tataggtaga taaacgactg aggagttgaa aaaatagata ggtagaagat
     3961 aaccgttaag ccttattctt agcggttatt tatattgttt aatagcgctg atatttcatc
     4021 aattatgcct gttttcgtgt ttctggtagt tgttcaagtt tattgctact atttttgatg
     4081 gtatgaatgt gcttataatg tatcccggtt aacgaaagtt ttggacttat actcat
//
LOCUS       DAVKFH010000098         3983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKFH010000098 DAVKFH010000000
VERSION     DAVKFH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.098"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(131..1546)
                     /locus_tag="VW006_002013"
     CDS             complement(131..1546)
                     /locus_tag="VW006_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9380800.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1559..3397)
                     /locus_tag="VW006_002014"
     CDS             complement(1559..3397)
                     /locus_tag="VW006_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9380801.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3415..>3983)
                     /locus_tag="VW006_002015"
     CDS             complement(3415..>3983)
                     /locus_tag="VW006_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9380802.1"
                     /translation="IIYINLTDHISSSIERYKEGIIISNPLRWEISKYYKEEFELGKR
                     ALQIIKKELGIELPIDEAAFIALHFVNANLENNFQESYKITEIIMGIEKIIQDFYCTE
                     FNQDSIDYYRFITHIKLFAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFI
                     QTEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1339 a          796 c          523 g         1325 t
ORIGIN      
        1 acaaaattaa attaattaat ccccctataa aataaagagg tttagaattt gatgtcttcc
       61 aaacctcttt atttcatatt taatgagatg ctgccttaac cgtgataata tctaatcatc
      121 aataaacaaa ctatttgaat aaggattctc catttgattc aatcactcct ttataccaag
      181 taaaggacat tttcttatat cgattgaatg tgccatttcc atcatcattt cgatcaacat
      241 aaatgagacc gtacctctta gaaagctgtg cagtggacat agaaacgcag tcaatacatc
      301 cccaagacgt atagcccata atttccacac catcttgtag agcttcagct acttgcaata
      361 aatgttcttt catatactga attctataat catcttggac tgttaagtta ttaaattcat
      421 cttttattag ttgatcttta gcacctaatc cgttttccac tataaataat ggaatttgat
      481 aacggtcata atatctattt aaaattatac gtagtccaat tggatcaatt tgccatcccc
      541 actcagaaga ctctaaataa ggatttacta aaccaccaat aatattccct tctcctgaat
      601 tatactgtgt tggaagagca gattgagtca cactcatgta atagctaaag gataaaaaat
      661 ctacagtata attttttaat aactctgcat cttcagctgc aaactctatg ttaatgtcat
      721 tttccttaaa atatcttttt gcataattcg gataataacc tctaacatgc acatctgaaa
      781 atagataatt tagattctca tactcatgag tcgcccatac atcttttgga tttggagtca
      841 ttggataagc tggcatagct aataccatac accccacctt aaactctgaa ttaatctcac
      901 gagcaatttt tgtaaccaaa ctcgaggcga ctaattcatg atgtatagct tgatataatt
      961 cttgtttcga aagattctcc ttaggtatat ctattcctcc actagtaaac ggtaattcca
     1021 aaacagagtt tacttcgtta aatgtaagcc aatatttaac tttatcttta tacctttcta
     1081 aaactgttcg agcaaatttt tcataaaaat gaatcattct cctatcaatc catccatgat
     1141 attttcttgc taaatataat ggagtctcat agtgtgaaag agttacaagt ggttctatcc
     1201 cgtgagcatg tagttcatca aacaattcat cataatattt caacccagct tcgttaggtt
     1261 cttcctcatc tccttttgga aaaattctac tccatgcaat agaagtacga aaaacattaa
     1321 agcccatttc agaaaacaag gatatatctt ccttatattt atgataaaaa tcaataccta
     1381 tcaattttaa gttatcttct gtaggatttt ctgttgcttc tcctaatcca cctttgggta
     1441 acacatcctg aactgataag cccttaccat cttcattata tgctccctct acttgattag
     1501 ctgcaacagc tccaccccaa agaaaatcat ctggaaaaat ggtcataact ttcctccatt
     1561 ataatattac cagtaattcc ttagaatgct cgattgtctg attattaggt aatactaata
     1621 catctagaaa atcattggta ttcgttacaa ttactggtgt aactgtttcg tagcctttag
     1681 tcttgattaa attcaagtcc atttcaaaaa tcaactgatt tttgaaaact ctgtctcctt
     1741 cttctacatg actaataaaa ccttgacctt ttagctcaac agtatctaat ccaatatgaa
     1801 ttagtaactc aacaccctca tcactcttca atccaattgc gtgcttagtc ggaaaaatat
     1861 ttgtaatttt cccatcaaat ggtgcataaa ccttaccttc acttggaata atcgctactc
     1921 cgtctccaat tagtttatct gaaaatgttt tatcctggac atcgcttaac ggaatgattt
     1981 ctcctgatat aggagaaaat atcatttttt tatttgaaac tccagcttta acttctaaat
     2041 tgctagaact ctcttcttca tcgattccaa atatataagc taatacaaag gtaataacaa
     2101 ccgaaataac cgccacaatt aaagcattta caatatttga tggcacatca gaataaataa
     2161 attgaggcaa cgctatcaaa gatgggacag caaatagata cgctttaaca ctagtaagac
     2221 ctgcaaataa tcccgctaat ccaccaccaa tcatagctgc ataaagcggt tttttatatt
     2281 ttaaagtcac accatataat gcaggttcgg taatccctgc aagtaaggct gagaaacctg
     2341 ctgcaaaagc aatttgtttt gtattattat ttttactctt taatgcaaca gccatcgaag
     2401 cagccccttg agctaagttt gaccctaaca ttgctggaag aattaatacg tctggagtag
     2461 caatagatgc cgccaaaaaa ataggtgcaa aagcccaatg cattccagtc ataacaataa
     2521 atggcataat agcaccaaga atagctaatg taagccatcc agctacacca tacatttgcc
     2581 caactagatt tgataatcct tcaccaacaa ttactccaat aggcccgact acaactaagg
     2641 caatacagct tgatactaat aatactagcg taggttgcaa aaaactctta gtaacagctg
     2701 gtgttaattt agcaatcatt ttttcaatat atttcatcaa ccaaaccata ataagaattg
     2761 gaacgactga cgaaccataa ctagctggtg tcacaggtac accaaataaa ctaagaggat
     2821 tccctgattg caccatttga acaaaatttg gatggagaag tacacctgct acagacatag
     2881 ctaatgtaga tgttactttt aatttttgtg atgcagaata agctaataac agcggtaaga
     2941 aataatatgg agcatcccca aaaaatgtca aaaaagcaat agtctgagaa tctgattgca
     3001 atataccaag cattggtaaa atgattacca agactttcaa catacctccc cctaacattg
     3061 ctggaatgat tggagtcatg gaaccagcga tatactcaat gattctttct aaaatattcc
     3121 ctttgtgccc ttgaacaact gaatcggatt caaaattgcc aagtttaacg aattctttat
     3181 aataattagc tacatcatta ccaagtataa tttgatattg tccattcttt ttcataatac
     3241 ctattacacc tggtatcttc ttcacatcat catcattgac taaattttca tcttttaatt
     3301 ctaatcttaa acgtgttaca caatgggtaa ctctattgac atttttttca cctccaatta
     3361 catcgaggat tttttgtacc ttatctttat aactcatggt attctcctat tctattaatc
     3421 taaatttttt gttaagcgac gaatatgagc catcaaataa actaattcac tagaagtcag
     3481 caaataattg tactcagttt gtataaacat tgccacctgt tcaccacatt catattctct
     3541 aggatattta tttttcatta atgctaacaa gtcttcatca tcatcgtcac aataagttgt
     3601 attttcaacc aagcgatggg caaataattt tatatgagtt atgaatctat aataatcaat
     3661 agaatcttgg ttaaactcag tacaatagaa atcttgaatg attttctcaa ttcccataat
     3721 tatttcagtg attttatacg actcttgaaa attattttct aaattagcat taacaaaatg
     3781 tagcgctatg aatgcagctt cgtcaattgg aagttcaata cctaactctt tttttattat
     3841 ttgtaaagcc cttttcccaa gctcaaattc ttctttataa tatttcgaga tttcccagcg
     3901 taggggattc gaaataataa tcccttcttt ataacgttct atgctcgaag aaatatgatc
     3961 cgttaaatta atatagataa ttt
//
LOCUS       DAVKFH010000099         3956 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKFH010000099 DAVKFH010000000
VERSION     DAVKFH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.099"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(251..862)
                     /gene="rpsD"
                     /locus_tag="VW006_002016"
     CDS             complement(251..862)
                     /gene="rpsD"
                     /locus_tag="VW006_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9380803.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1057..2109)
                     /locus_tag="VW006_002017"
     CDS             complement(1057..2109)
                     /locus_tag="VW006_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9380804.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2106..2804)
                     /locus_tag="VW006_002018"
     CDS             complement(2106..2804)
                     /locus_tag="VW006_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9380805.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGCETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2971..>3956)
                     /gene="pavB"
                     /locus_tag="VW006_002019"
     CDS             complement(2971..>3956)
                     /gene="pavB"
                     /locus_tag="VW006_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9380806.1"
                     /translation="VKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVY
                     GNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVKDSIDVPAAYLEKAKGEGP
                     FTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYF
                     YQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKI
                     NVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASA
                     NKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT         1069 a          874 c          775 g         1238 t
ORIGIN      
        1 gttatactac ctgttcgctc acaataagag agaacttttt tacgaaaatc tattgaatat
       61 gccataaaaa gattatacca cattgtgtac tatttttggt tcactttact atatcacttt
      121 ggactatatc ggtctagtta tgtctaaacg attatagctg gatactaaga gaaatcaaaa
      181 aagcactaag ggagaacgtc ccaaagtgct taatatcaag gcctcaaagc ctatcttatt
      241 taataaaata ttacaacatt ttgttgtaga attcaacgac aagtgcttcg ttgatttctg
      301 ggttgatttc gtcgcgttct ggcaagcgag tcaatgaacc ttccaacttt tcagcgtcga
      361 atgatacgaa tgctggacgt ccaagagtag cttctactgc ttcaaggatt gctggaactt
      421 tcaatgattt ttcacgaact gagatcactt gacctggagt tacgcggtat gatgggatat
      481 caacgcgttt cccgtcaaca aggatgtgac cgtggtttac gaattgacga gcttgacgac
      541 gagtagtcgc gagaccaaga cggtaaacaa cattatccaa acgacgttcc aaaagaagca
      601 taaagttgaa acctaggatt ccacctttga tttttgtagc ttgtacgaac aagttacgga
      661 attgtttttc acctacaccg taagtgaaac gaagtttttg tttttcagcc aattgcaaac
      721 catattctga caatttagaa cggttgtttg gtccgtgttg tcctggtaca tagttacgac
      781 gtgccaattc tttacctgta cctgtaagtg aaaggccaag gcgacgagct tgtttccaag
      841 atggtcctgt ataacgtgac atatgaatgt cctcctgata taaataatat ttcggtggaa
      901 atagtcactt agaaagccct gattcgtgca gatgcccttc gcctaaacag ccaaggttac
      961 ttgtcataag acacctgttg acgagcttca tgctttcctg ctgatatttc acactaaatt
     1021 cattttacca tgaatagctg gatttgtaaa ggggttttag gctttatttt cactaccaga
     1081 gaggttgaga acgagggtaa aggtacttcc tagaccgtac tggctgctga ctgtgatttc
     1141 cccacccaat tgatgggcca attcacgcgc aatcgcaagt cctaatccat gaccacctgt
     1201 cttcatgtta cgcgaagttt cgacacgata aaggcgtttg aaaatatttt ccaaatcctc
     1261 tggggcaata ccctgccctt catcggtcac actgattgaa agctggtcct tctccagctt
     1321 agccaccact tccagcttgg ttcctggagc agaatattta aaagcgttat cgaccagatt
     1381 caccaagata cgagaaagct tagcataatc tccctcaatc cgggcagact ctgggattac
     1441 ctgcaagtgg acatctcttc tctcctgctc aatcaaaaac tgaaattcac tcatgcactc
     1501 aattaagagc ttgtccagaa aaatactgtc tttactggta gtttccacct gatttctagc
     1561 tgtgtttagg gtcaaaaaat tcaactcctc aaccagttta ttgagcctct ccgtctggcg
     1621 tccaatggtt gctagataat gagcttgctc cgactccttg ataataccat ccaaaatccc
     1681 ttctaccgtc gcttggatcg aagtgatagg agtcttaata tcatgcgaca actgggcaat
     1741 catcaagccc ttttctcgtt cgctttcttc caaggaatca aagcttacct gcaaatcatg
     1801 ggacatctca ttaaaagttt gccctaattg ctgaaattct acaggacctt gaacctccaa
     1861 atttgaagga aaatccttgg ccgctacccg cttggcatgc tccttgagtt tgcccaacga
     1921 cgtaaagact ggcaatagga gaaagagact aatcccagca ccgacaaggc tggcaacgat
     1981 ggtcatccca agcaaaaagt aaatctcgcc tttcgcaatc agcatttttt gaacagccca
     2041 aaaaacaacc aaaatggtta agagggttga aataatatat ccaaccaaaa tataactttt
     2101 tagtttcatg tttgtcctct cggtttctct atcttatatc ccaaccccca aactgtctta
     2161 atagtgggcg tttggtcact actatatttt gccagctcct gtcgaagagc atggatatgc
     2221 acattcaagg tattggtgtc atccacgtag tcttctttcc agatcttttc atagaggtct
     2281 gtcttggaga aaactcgctc tggattgcta gctaaaatcc acagcaattc aaatgattta
     2341 acagttaaat ccagcatttc ttctcctatt tgaacttcat gactactatg attcattttt
     2401 agattgccaa gggaaatcag ctctgtttcg cacccacgat gaaggcggcg caaaatattg
     2461 tggacacgca aaactagctc acgtgggcta aaaggcttag caataaaatc atctgctccc
     2521 aagctcaggc cgtaaatctt gtcctgttca ctggtcttag cagtaataaa taggaaaggc
     2581 tgctctggtg ataagtattg aacctcactg attaaatcat aaccatccat ccgaggcatc
     2641 atgacatctg tgataatcaa atcaatcggt tttttcttga acagctctag cgcttccagt
     2701 ccatcatggg ctaccaagac ctgataacct gcctgaatta agtatctctg atgaatatct
     2761 gtgatttcta cctcgtcgtc aacgagtaaa attgtctttc ccatctgtct ctcctttgat
     2821 aaaaacagtg ctatactgct ttaagtataa cactattttt attgttttac gatgatttta
     2881 atctgctatt gaaagaaagt cctgattgct gattgagttg ttaaactaat cttagtcagc
     2941 ccactatcca tcatttattt ccttagctga ttagttttct tcttttttgt ttttcaaacc
     3001 tagaccaccg agtaaacctg caagcgcaag cccaaggaaa ccaatacttg ccattgatgt
     3061 ttgagtctca ccagtatttg gtagcatagc tttatcctct gacatcatcg tatcagacat
     3121 cttgttagca gaagcagcca tgttttcacc tgccatcgtg ttggtagaac ttgtcatggt
     3181 gtcagcaggc atgctatctg tagtacctgt agcatgattg tgattcatcg gagtcacgcc
     3241 agaaccagag ttagaaggtg ataatgaacc atttgctgtg tctgatgttt ctttaacatt
     3301 tatcttaata gtgacttttt tagttgctac gatgttgtcc aagtctggtt taccgtcttt
     3361 gttaccatag acattgactg tagcgctgta agtttgagta ccgtttgctc ggaactggtc
     3421 aatgagcgct tgtttttctt tgccagctac atttccgtcc aaggctactt gatagaagta
     3481 ttgacctttg gtcttcacgt tttcgcctag tggagatagg gctgggtttt tagcgtcgcc
     3541 gttatctgac catggtgcct tgtcagatgc cttgagcaag agacgagtca acatgccatc
     3601 acctgcgaag agttcgtatg gaatcacatg gttgacacct gctgtgaatg gaccttcacc
     3661 cttggctttt tctaagtagg ctgctggaac atcgatactg tctttaacgt tgtctgcaac
     3721 ggctttttga actgtttctt tagaaattaa accgtttatg ttaatagtga cttttttagt
     3781 tgctacgatg ttgtccaagt ctggtttacc gtctttgtta ccatagacat tgactgtagc
     3841 gctgtaagtt tgagtaccat ttgctcggaa ctggtcaatg agcgcttgtt tttctttgcc
     3901 agctacattt ccgtccaagg ctacttgata gaagtattga cctttggtct tcacgt
//
LOCUS       DAVKFH010000100         3947 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKFH010000100 DAVKFH010000000
VERSION     DAVKFH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.100"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            69..521
                     /locus_tag="VW006_002020"
     CDS             69..521
                     /locus_tag="VW006_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380807.1"
                     /translation="MEFNINSKEFSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1353..1712
                     /locus_tag="VW006_002021"
     CDS             1353..1712
                     /locus_tag="VW006_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380808.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            1722..2051
                     /locus_tag="VW006_002022"
     CDS             1722..2051
                     /locus_tag="VW006_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9380809.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQV"
     gene            2074..3947
                     /locus_tag="VW006_002023"
                     /pseudo
     CDS             2074..3947
                     /locus_tag="VW006_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT         1456 a          579 c          772 g         1140 t
ORIGIN      
        1 ttgaaaaaag gttctcactt atcaggtgca tataaacgaa aaattttttc taaagaaaat
       61 acacgatttt ggagtttaat attaactcga aagaattttc gcaacaactt gatattctct
      121 ataaagaagt tggtgtatta agtttgggac aaaaagttgt agccatgctt gattttttat
      181 tagcatatag tgattattct aaagacttca gaccattgat tattgatcag cctgaagaca
      241 atctagacaa tcgttatatt tacaggcatt tagttcagca gtttagagat gtgaaagctc
      301 aacgtcaaat tattttagca acacataatg ctacaattgt aacaaattct atgacagatc
      361 aagttgttat tatggagtca gatggagtta acggatggat tgaatcacag ggatatgtta
      421 gtgaaaaata tataaaaaat catatcatca atcaattaga gggaggaaaa gattccttca
      481 agcataaaat gtctatatat gagacggctt tatcagagta gagtcagaaa aagtaggtta
      541 gaaatttagc ctactttttt ctttgtccga caggcatagt gtacatctga ggtccaagtc
      601 ctctgtggat atttgctgca gatgaaacca atagcgactc ctaagcctga atatcgtgag
      661 gtagggggga taggaaggaa ttagcgaaat caaggttcta caaacagaat cgtgacttga
      721 agccatatat agcggatgag gaactctaaa atccaaatag gtgtcgtaac ctatatacgt
      781 aaattacgag agtaaactag gaaagatgta cggcttattc cgtgagcgtt taggacgtag
      841 tacaacgaat catgggagtc agctgaacac atagtattga agaaatttct gtaatggaaa
      901 tggagcgaag aagtgaacaa ttaaatgaat acctctctaa ttaaatttgt caattctaat
      961 tcctggtatg aaaagacagt gacctgaaaa tgtaaacgat gggagctgat cataaatata
     1021 ggacggtaca tgcagtggtg ttagagatta gtccttactt gatttgtgat aacttcccca
     1081 aatttcttct gctatacttt tctcaacttt taaaaatcca actaagaatt ttacctgggg
     1141 gtttgggggc ggagccacta agttatctta tcgttagctg tcaaaactgg taggttttga
     1201 taggctggcg atatgatttt tgggatattg tggacacaat atctgagctc gcaaagaccg
     1261 aacaaggaga gactgaggtc tttaggaatc gtactgacaa tgtgaggcag ccttacattg
     1321 tcagacaagt ataatgttga aagaatggta agatgggaca agaaattaag ttaatccgta
     1381 agcaatttag aatcacgaga caagaagaaa aacagataaa agaaatgatg agggaacaaa
     1441 aagtggatag tttctcagaa tttcttcgtc aaaatttatt gaaaaagaat tatcaggata
     1501 gaatttttga aagttggttc tccctttggc agtctcaaaa gtttgaacag attagtcgag
     1561 atgtgtatga agttctggtt gtcgcaagag aaaatcatca agtgactcaa gagcacgtct
     1621 caatcttatt gacttgcgtt caagaattga ttgcagaagt gaatcaagta cagccactca
     1681 gtcgtgagtt tcgtgaaaaa tatatgggtt aggaggatgt aatggtttat cgctatcgta
     1741 ccaatctcaa aaaagtattt ctaactgatc cagaattaca ccaattgaat gaacggattg
     1801 ctaagagcaa ttgtcaaaat ttttcagtct atgccagaaa agttttgctt aatcccaata
     1861 tgtcatttgt cacgattaac actgatactt atgaccagtt agtttttgaa ttgagacgta
     1921 ttggaaataa catcaatcaa attgcgcgtg cgattaatca aagccatctg atttctcagg
     1981 accaattaca agaattgagt aaaggagtcg gagagttaat taaggaagtg gacaaagaat
     2041 ttcaagtgta ggtgaaaaga ctgaaggagt ttcatggtag ttactaaaca ctttgcgacg
     2101 cacggaaaaa aatatcgtag gcgtttgatt aaatatatcc tcaatccaga taaaacagat
     2161 aatctaaaat tagtatctga ttttggcatg agtaattact tagattttcc tagccataca
     2221 gaaatggtag aaatgtacaa tgttaacttt actaataacg acaagttgta cgagtccaga
     2281 aatgaccgac aagaaaaaca ccaacaaact attcatgccc atcatctcat ccaatcattt
     2341 tctcctgaag ataatctgac acctgaagaa attaaccgca ttggttatga gaccatgatg
     2401 gaattgacag gaggtcgttt taagtttatc gtagcaactc acacagacaa agatcatgtt
     2461 cataatcaca tcctaatcaa cgctattgac cgcaattcag ataaaaagtt gatatggaac
     2521 tatgccttgg aacgaaacct ccgtatgatt tcagaccgta tttctaaaat ggctggtgca
     2581 aaaattattg agaaacgtta ttcttatcga ggttataaaa aatataggga atctagtcat
     2641 aaatttgaat tgaaacaacg tctttatttt ttgatgcagt aatctaaata gtttgatgat
     2701 tttttagaga aagcaaagca gttgcatgtt cagattgatt ttagtcaaaa gcatagtcga
     2761 ttcatgatga cggatcgagc aatgacaaaa cctattcgag gacgccaact cagcaaacga
     2821 gatctatatg atgaagaatt ttttagaacg cattttgcta agcaagagat tgaaagtcgt
     2881 ttagaatttt tgttgaaccg tgtcaattct ttagaagagt taataacaaa agcaaaagaa
     2941 ttgaatctaa ccattgactt aaaacaaaaa aatgtaactt ttatcctgaa agaaaataat
     3001 caaaagataa gtttgggtca tcaaaaaata agtgataaga aattatatga tgtcaaattt
     3061 tttcaagatt attttaaaaa taaggaagtc attgcttcag aaggattaga gaatttacag
     3121 gaacagtacc atgctttcca agaagaacga gataaggata aagtatccac tgaagagatt
     3181 gaggaagcct ttaagacatt taagaagcct ttaagacatt taaggaaaaa tgagataccg
     3241 ttcgtgaatt tgaagtggaa cttgcagaga accaaataga gaagctagtt gataagggcg
     3301 tctatatcaa ggtgtctttc ggtgtcaaat agagcggtct tattttcatt cccaactatc
     3361 aattggatat tatggaagaa gaaaatcata aaaaatataa agtctacatt cgagaaacgt
     3421 cttcttactt tgtctataat aaagaaaaca tggataataa ttgtttcatc aagggtagga
     3481 ccttaatcag acaattgagc aacgatagcc aaaaactccc ctatagacga ccaactttaa
     3541 aaagtcttca agagaagatt tctgaaatta atcttatgat tgaactatct aacacgaaca
     3601 aacaatacca agaaatcaaa gatgagcttg tattagaaat tgcagagatt gatatgaagc
     3661 tagaagaaac ccaagaaaaa atcgccacct taaacaagat ggcggaagtc cttatcaatt
     3721 tgaagagtga agatgagatc ggtagaaaat tagctaagta tgattttgac caaatgaaca
     3781 tgacggaatc aataatgtta gatcgactca atacatatat tttaaaatta caacaggaac
     3841 ttagtaatga aattaataaa tatgaggaga tagctagaag attggatttg tttgtgaaaa
     3901 ttattaatac caataaattt acagttctaa aatttcacga gaatgcc
//
LOCUS       DAVKFH010000101         3910 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKFH010000101 DAVKFH010000000
VERSION     DAVKFH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.101"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            332..559
                     /locus_tag="VW006_002024"
     CDS             332..559
                     /locus_tag="VW006_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380810.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            847..3729
                     /gene="nanA"
                     /locus_tag="VW006_002025"
     CDS             847..3729
                     /gene="nanA"
                     /locus_tag="VW006_002025"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9380811.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKISRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQKIIGIAKGSIESM
                     HNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKESDSLVTLTTK
                     EDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1333 a          650 c          864 g         1063 t
ORIGIN      
        1 tcatgcggct agcttcttag tttgctcttt gattttcatt gagtataaaa acagatgagt
       61 ttctgttttc tttttatgga ctataaatgt tcagctgaaa ctactttcaa ggacattatt
      121 atataaaaga attttttgaa actaaaatct actatattac actatattga aagcgtttta
      181 aaaatgaggt ataataaatt tactaacgct tataaaaagt gatagaatct atctttatgt
      241 atatttaaag atagattgct gtaaaaatag tagtagctat gcgaaataac agatagagag
      301 aagggattga agcttagaaa aggggaataa tatgatattt aaggcattca agacaaaaaa
      361 gcagagaaaa agacaagttg aactactttt gacagttttt ttcgacagtt ttctgattga
      421 tttatttctt cacttatttg ggattgtccc ctttaagctg gataagattc tgattgtgag
      481 cttgattata tttcccatta tttctacaag tatttatgct tatgaaaagc tatttgaaaa
      541 agtgttcgat aaggattgag caggaagtat ggtgtaaata gcataggctg atgtccatca
      601 tttgcttata aagagatatt ttagtttaat tgcagcggtg tcctggtaga taaactagat
      661 tggcaggagt ctgattggag aaaggagagg ggaaatttgg caccaatttg agatagtttg
      721 tttagttcat ttttgtcatt taaatgaact gtagtaaaag aaagttaata aaagacaaac
      781 taagtgcatt ttctggagta aatgtcttat ttcagaaatc gggatataga tatagagagg
      841 atcagtatga atcggagtgt tcaagaacgt aagtgtcgtt atagcattag gaaactatcg
      901 gtaggagcgg tttctatgat tgtaggagca gtggtatttg gaacgtctcc tgttttagct
      961 caagaagggg caagtgagca acctctggca aatgaaactc aactttcggg ggggagctca
     1021 accctaactg atacagaaaa gagccagcct tcttcagaga ctgaactttc tggcaataag
     1081 caagaacaag aaaggaaaga taagcaagaa gaaaaaattt caagagatta ctatgcacga
     1141 gatttggaaa atgtcgaaac agtgatagaa aaagaagatg ttgaaaccaa tgcttcaaat
     1201 ggtcagagag ttgatttatc aagtgaacta gataaactaa agaaacttga aaacgcaaca
     1261 gttcacatgg agtttaagcc agatgccaag gctccagcat tctataatct cttttctgtg
     1321 tcaagtgcta ctaaaaaaga tgagtacttc actatggcag tttacaataa tactgctact
     1381 ctagaggggc gtggttcgga tgggaaacag ttttacggta attacaacga tgcaccctta
     1441 aaagttaaac caggtcagtg gaattctgtg actttcacag ttgaaaaacc gacaccagaa
     1501 ctacctaaag gccgagtgcg cctctacgta aacggggtat tatctcgaac aagtctgaaa
     1561 tctggcaatt tcattaaaga tatgccagat gtaacgcatg tacaaatcgg agcaaccaag
     1621 cgtgccaaca atacggtttg ggggtcaaat ctacagattc ggaatctcac tgtgtataat
     1681 cgtgctttaa caccagaaga ggtacaaaaa cgtagtcaac tttttaaacg ctcagattta
     1741 gaaaaaaaac tacctgaagg agcggcttta acagagaaaa cggacatatt cgaaagcggg
     1801 cgtaacggta aaccaaataa agatggaatc aagagttatc gtattccagc acttctcaag
     1861 acagataaag gaactttgat cgcaggtgca gatgaacgcc gtctccattc gagtgactgg
     1921 ggtgatatcg gtatggtcat cagacgtagt gaagataatg gtaaaacttg gggtgaccga
     1981 gtaaccatta ccaacttacg tgacaatcca aaagcttctg acccatcgat cggttcacca
     2041 gtgaatatcg atatggtgtt ggttcaagat cctgaaacca aacgaatctt ttctatctat
     2101 gacatgttcc cagaagggaa gggaatcttt ggaatgtctt cacaaaaaga agaagcctac
     2161 aaaaaaatcg atggaaaaac ctatcaaatc ctctatcgtg aaggagaaaa gggagcttat
     2221 accattcgag aaaatggtac tgtctataca ccagatggta aggcgacaga ctatcgcgtt
     2281 gttgtagatc ctgttaaacc agcctatagc gacaagggtg atctatacaa gggtaaccaa
     2341 ttactaggca atatctactt cacaacaaac aaaacttctc catttagaat tgccaaggat
     2401 agctatctat ggatgtccta cagtgatgac gacgggaaga catggtcagc gcctcaagat
     2461 attactccga tggtcaaagc cgattggatg aaattcttgg gtgtaggtcc tggaacagga
     2521 attgtacttc ggaatgggcc tcacaaggga cggattttga taccggttta tacgactaat
     2581 aatgtatctc acttaaatgg ctcgcaatct tctcgtgtca tctattcaga tgatcatgga
     2641 aaaacttggc atgctggaga agcggtcaac gataaccgcc aggtagacgg tcaaaagatc
     2701 cactcttcta cgatgaacaa tagacgtgcg caaaatacag aatcaacggt ggtacaacta
     2761 aacaatggag atgttaaact ctttatgcgt ggtttgactg gagatcttca ggttgctaca
     2821 agtaaagacg gaggagtgac ttgggagaag gatatcaaac gttatccaca ggttaaagat
     2881 gtctatgttc aaatgtctgc tatccatacg atgcataatg gaaaagaata cattatcctc
     2941 agcaatgcag gtggaccgaa tcgtgaaaat gggatggtcc acttggcacg tgtcgaagaa
     3001 aatggtgagt tgacttggct caaacacaat ccaattcaaa aaggagagtt tgcctataat
     3061 tcgctccaag aattaggaaa tggggagtat ggcatcttgt atgaacatac tgaaaaagga
     3121 caaaatgcct ataccctatc atttagaaaa tttaattggg aatttttgag caaagatctg
     3181 atttctccta ccgaagcgaa agtgaagcga actagagaga tgggcaaagg agttattggc
     3241 ttggagtttg actcagaagt attggtcaac aaggctccaa cccttcaatt ggcaaatggt
     3301 aaaacagcga ctttcctaac ccagtatgat agcaagacct tgttgtttgc agtagataag
     3361 gaagatatcg gacagaaaat tattggtata gctaaaggaa gcatcgaaag tatgcataat
     3421 cttcctgtaa atctagcagg tgccagagtt cctggcggag taaatggtag caaagcagcg
     3481 gtgcatgaag ttccagaatt tacaggggga gttaatggta cagagccagc tgttcatgaa
     3541 atcgcagagt ataaggaatc tgattcgctt gtaactctta ctacaaaaga agattatact
     3601 tacaaagctc ctcttgctca gcaggcactt cctgaaacag gaaacaagga gagtgacctc
     3661 ctagcttcac taggactaac agctttcttc cttggtctgt ttacgctagg gaaaaagaga
     3721 gaacaataag agaagaattc taaacatttg attttgtaaa aatagaagga gatagcaggt
     3781 tttcaagcct gctatctttt tttgatgaca ttcaggctga tacgaaatca taagaggtct
     3841 gaaactactt tcagagtagt ctgttctata aaatatagta gattgaaata agatgtgaac
     3901 aactctatca
//
LOCUS       DAVKFH010000102         3834 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKFH010000102 DAVKFH010000000
VERSION     DAVKFH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.102"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            317..1696
                     /locus_tag="VW006_002026"
     CDS             317..1696
                     /locus_tag="VW006_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9380812.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            1710..2375
                     /locus_tag="VW006_002027"
     CDS             1710..2375
                     /locus_tag="VW006_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9380813.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            2489..2584
                     /locus_tag="VW006_002028"
                     /pseudo
     CDS             2489..2584
                     /locus_tag="VW006_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2820..3359
                     /locus_tag="VW006_002029"
     CDS             2820..3359
                     /locus_tag="VW006_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9380814.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLREFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            3426..>3834
                     /locus_tag="VW006_002030"
     CDS             3426..>3834
                     /locus_tag="VW006_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9380815.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLY
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
BASE COUNT         1016 a          726 c          884 g         1208 t
ORIGIN      
        1 acagtgtttt gagctgactt cgtcagttct atccacaacc tcaaaacagt gttttgagca
       61 acctgtgact agctttctaa tcgatgcctt ggttttcatt gcctataatc aaaaagagaa
      121 attttctcct gaaaagcata tagagtagct ggcgttaaaa gctcctgtct tgcttttttg
      181 acctatagtc atatctatca agtattgttc ttgcctaagc tatcaataaa aaggtggcat
      241 tttttaggct tggtgttagt gaattttgcc ttatcctatc taagtcattt cgagcttttt
      301 atggtacaat ggaaacatgt tattcaaatt atctaaggaa aaaatagagc taggcttatc
      361 tcgtttatcg ccagcccgtc gtattttttt gagttttgcc ttggtcattt tactaggctc
      421 tcttcttttg agcttgccct ttgtccaagt tgaaagctca cgagcgactt attttgatca
      481 tcttttcact gctgtctctg cagtctgtgt gacaggtctc tcaacccttc cagtagctca
      541 cacctataat atctggggcc aaataatctg tttgctcttg attcagatcg gtggtctagg
      601 gctcatgacc tttattgggg ttttctatat ccagagcaag caaaagctta gtcttcgtag
      661 ccgtgcaact attcaggata gttttagtta tggagaaact cgatctttga gaaagtttgt
      721 ctattctatt tttctcacga cctttttggt tgagagcttg ggagctattt tgcttagttt
      781 tcgccttatt cctcaacttg gctggggacg tggtcttttt agttccattt ttctagcgat
      841 ctcagccttc tgtaatgccg gttttgataa tttagggagc accagtttat ttgcttttca
      901 gaccgattta ctggtcaatc tggtgattgc aggcttgatt attacaggcg gccttggttt
      961 tatggtctgg tttgatttgg ctggtcatgt aggaagaaag aaaaaaggac gtctgcactt
     1021 tcatacgaag cttgtattat tattgactat aggtttgttg ttatttggaa cggcaactac
     1081 tctctttctt gagtggaaca atgctggaac gattggcaat ctccctgttg ccgataaggt
     1141 tttagttagc ttttttcaaa cagtgacgat gcgaacagct ggcttttcta cgatagatta
     1201 tactcaggct catcctgtga ctcttttgat ttatatctta cagatgtttc taggtggggc
     1261 acctggagga acagctgggg gactcaagat tacgacattt tttgtcctct tggtctttgc
     1321 acgaagtgag cttctaggct tgcctcatgc caatgttgcg agacgaacga tcgcgccgcg
     1381 aacggttcaa aaatccttta gtgtctttat tatctttttg atgagcttct tgataggatt
     1441 gattctgcta gggataacag ccaaaggcaa tcctcccttt atccacctca tatttgaaac
     1501 catttcagct cttagtacag ttggtgtaac ggcaaatctg actcctgacc ttgggaaatt
     1561 ggctctcagt gttatcatgc cacttatgtt tatgggacga attggtccct tgaccttgtt
     1621 tgttagcttg gcagattacc atccagaaaa gaaagatatg attcactata tgaaagcaga
     1681 tattagtatt ggttaagaaa ggaaagagca tgtcagatcg tacgattgga attttgggct
     1741 tgggaatttt tgggagcagt gtcctagctg ccctagccaa gcaggatatg aatattatcg
     1801 ctattgatga ccacgcagag cgcatcaatc agtttgagcc agttttggcg cgtggagtga
     1861 ttggtgacat cacagatgaa gaattattga gatcagcagg gattgatacc tgcgataccg
     1921 ttgtagtcgc gacaggtgaa aatctggagt cgagtgtgct tgcggttatg cactgtaaga
     1981 gtttgggggt accgactgtt attgctaagg tcaaaagtca gaccgctaag aaagtgctag
     2041 aaaagattgg agctgactcg gttatctcgc cagagtatga aatggggcag tctctagcac
     2101 agaccattct tttccataat agtgttgatg tctttcagtt ggataaaaat gtgtctatcg
     2161 tggagatgaa aattcctcag tcttgggcag gtcaaagtct gagtaaatta gacctccgtg
     2221 gcaaatacaa tctgaatatt ttgggtttcc gagagcagga aaattcccca ttggatgttg
     2281 aatttggacc agatgacctc ttgaaagcag atacctatat tttggcagtc atcaacaacc
     2341 agtatttgga taccctagta gcattgaatt cgtaaagagg gatgacccct cttttttgat
     2401 gcttaagatg gcaaatagag acagaagccc cttgtcttct agtaaaagtt cttcaaaggc
     2461 tggactttat ggtaaaatag aaggaagtga caagagagag taatactcaa tgaaaatcaa
     2521 agatcaaact aggaaactag ctgcgggctg ctcaaaacac tgttttgagg ttgtggatag
     2581 aactgacgaa gtcagctcaa aacactgttt tgaggttgca gatagaactg acgaagtcag
     2641 ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagctcaa aacactgttt
     2701 tgaggttgca gatagaactg acgaagtcag taacatctat acggcaaggc gacgttgacg
     2761 cggtttgaag agattttcga agagtataag aaaaaatcag tcccctaaag gagcagatta
     2821 tgaagttatt gtctatcgcc atttctagct ataatgcagc agcctatctt cattactgtg
     2881 tggagtcgct agtgattggt ggtgagcaag ttgggatttt gattatcaat gacgggtctc
     2941 aggatcagac tcaggaaatc gctgagtgtt tagctagcaa gtatcctaat atcgttagag
     3001 ccatctatca ggaaaataaa ggccatggcg gtgcggtcaa tcgtggcttg gcagaggctt
     3061 ctgggcgcta ttttaaagta gttgacagtg atgactgggt ggatcctcgt gcctacttga
     3121 aaattcttga aaccttgcgg gaatttgaga gcaagggtca agaggtggat gtctttgtga
     3181 ccaattttgt ctatgaaaag gaaggtcagt cttgtaagaa gagtatgagt tacgattcag
     3241 tcttgcctgt tcggcagatt tttggctggg accaggtcgg aaatttctcc aaaggccagt
     3301 atatcatgat gcactcgctg atttatcgga cagatttgtt gcgtgctagc cagttctaat
     3361 tgcctgaaca tactttttat gtcgataatc tctttgtctt tacgcccctt cagcaggtca
     3421 agaccatgta ctatctgcct gtcgatttct atcgttattt gattgggcgt gaggaccagt
     3481 ctgtcaatga gcaagtgatg attaagtgca ttgaccagca actcaaggtc aatcgactct
     3541 tggtagacca actttatttg tcccaagtga gtcatcccaa aatgcgagaa tatctgctga
     3601 atcatattga aatcacgacg gtgatttcca gtaccctgct caaccgatct ggaacagcgg
     3661 agcatctggc aaaaaaacgc caattgtgga cctatattca gcagaaaaat ccagaagtct
     3721 ttcaggctat tcgtaagacc atgttgagcc gtttgaccaa acattctgtc ttgccagatc
     3781 gcaaactgtc caatgtcgtc tatcaaatca ccaaatctgt ttatggattt aatt
//
LOCUS       DAVKFH010000103         3803 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKFH010000103 DAVKFH010000000
VERSION     DAVKFH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.103"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..418
                     /locus_tag="VW006_002031"
     CDS             <1..418
                     /locus_tag="VW006_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9380816.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VW006_002032"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VW006_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9380817.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1425 a          624 c          746 g         1008 t
ORIGIN      
        1 taaaaataag gcagaccaag acagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaa
//
LOCUS       DAVKFH010000104         3748 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKFH010000104 DAVKFH010000000
VERSION     DAVKFH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.104"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..255
                     /locus_tag="VW006_002033"
     CDS             <1..255
                     /locus_tag="VW006_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380818.1"
                     /translation="LIFLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIVYLF
                     LGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEFELFKNSK"
     gene            1390..1893
                     /locus_tag="VW006_002034"
     CDS             1390..1893
                     /locus_tag="VW006_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380819.1"
                     /translation="MKLLDCILDYQERFNRKACQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILLYNDVIERISLYEFIGDIFYFKITSCC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            2179..>3748
                     /locus_tag="VW006_002035"
     CDS             2179..>3748
                     /locus_tag="VW006_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEU9380820.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEEKAKKAEEAAKEVDDASLA
                     VQKAYVEQRKAQEEKNKHSGNPEYAKKLAEADKKITEETTKLTDAQTKFQSVQAMVVP
                     EPEQLAETKKKAEEAKAEEKVAKRKYDYATLKVVLAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADPEDDTAA
                     LQNKLATKKAELAKKQTELEKLLDSLDPEGKTQDELDKEAAEAELDKKVEALQNKVAD
                     LEKEISNLEILLGGADSEDDTAALQNKLATKKAELEKTQKELDAALNELGPDGDEEET
                     PAPAPQPEQPAPAPAPKPEQPAPAPAPKPEQPAPAPAPKPEQPAPAPKTGWKQENGMW
                     YFYNTDGSMATGWLQ"
BASE COUNT         1384 a          538 c          736 g         1090 t
ORIGIN      
        1 cttatctttt tgataaaaat ttgtgtagat actacgataa aatttttatt catcagatta
       61 tttgacacaa gtactttaat gaagaggaga atattttttc ttttcgcatt ggatgttata
      121 gtttacttat ttttaggata tcttttagtg attcaaaaag caggctattt gttttcggtt
      181 ttacttcttt tttctaattt ttcagttcca tttatcaaag aaaaggaatt cgaattattt
      241 aaaaatagca agtaatagaa acataggtta aatcttagaa cggcttaaaa tcagatatga
      301 taaagggaaa tagtcttatc taggtaacta agtgctacaa acacagtcga ggatcacttg
      361 ttaggattac attttaatca tgcttaataa gaatggagaa ttcctatggg gattgtatta
      421 ctctgaagtt tcgaatacct tagttctcgt gattttgagc tgttctagct ttagaatcct
      481 tcagaacgta aaattttaag cagtcaaaga cttagaatag cctggaaaca tttattttat
      541 agcaattcta aacttagaat tgctcatata tatgtatttg agctgctcga tagttaggac
      601 agctctattc tgagaaatct gagctatcaa tgcttcagaa tagtatttcg ccaatggttt
      661 ttattgaaga ggtgattgtg ttatgatagt ctaaaataaa attgatttaa tagagtaaat
      721 ggagaatcgt tacatgaaac atttttttgc aggaattggt gagataaaac ttgttggcta
      781 cttattgtat atatgtgtag gactagcccc tctttttcat atatatgtca taggttcgga
      841 actgagtttt gtgaaaatag tgttatctat tttgggagtt atatttgtaa gtatgttaac
      901 tattgctcga atttatcgga cttttctata aagaatagtt tggacttgaa ctgtatctaa
      961 aaacagaggt tggtcgatta tcagcctctt tcttgtcgtt gaaaagagtg agatatattg
     1021 acttttgaaa aaaagatgtc ataattgagt tgggataaac ctaaaagtaa tatagtagat
     1081 tgaatctgga atagtccgtt atgactgtta aaatatttct agaaattaat ttgactttcc
     1141 taatcgattt gttcacatct tgtttcaatc tactatactt tctcgttaaa ccgttcggca
     1201 attcatggtg acatggacac atagtagtaa gcacactatg tggcttggca gagctaaaaa
     1261 ctgttccctt gcgataagcc taataagcac aacataggga gttagagaag ccgactctaa
     1321 tcaaccactt tgggcagtag tgagaactgc cccgtgcttt tttattttga gaaaatatgg
     1381 agtttgtcgt tgaaattact tgattgtatt ttggattatc aagaaagatt caatagaaaa
     1441 gcatgtcaag tatcaacgaa ttataagtat ttagagactt tcaaagtgaa tttttgcctg
     1501 actgatttac atcatttatt tggcttacac aaaatcacac gagattatgc tagtcaaaca
     1561 aaacctgcta ttcaagctgg tgtttttatt ttagaagatt ttagaaatat cctattgtac
     1621 aatgatgtaa tcgaaaggat atctttatat gagtttatag gtgatatttt ctattttaaa
     1681 ataacaagtt gttgcatcgt agctaaggat ttatctaaaa atactatgaa attggacgtc
     1741 atattttttg aggataaaaa taaaagatcc gcagttttag gtttacgaag agacaaaagc
     1801 ggagtattta aaccagttac tctacatttt acaagcgcta agaaatatgc taaagttcgt
     1861 aaaacagatg tgaaagaaat gaaatggcta taaagattgt ccgcaggctt aagcttgcgt
     1921 tttttgttgt ttaacgaaat taaaaaacaa gctctctcat cggaaatgtt tttataggaa
     1981 tgaaggaaga tgatgctatg tttaaaaaga gaatcgaaaa gggcaaaagt agtgtcttta
     2041 tctttttgga gtagaagctt atgatataga aatttgtaat aaaaatgtaa tataaaacat
     2101 ttgacaaata tttacggggg gagtatactt aatataagta tagtctaaaa atgactatca
     2161 gaaaagaggt aaatttagat gaataagaaa aaaatgattt taacaagtct agccagcgtc
     2221 gctatcttag gggctggttt tgttacgtct cagcctactt ttgtaagagc agaagaagct
     2281 ccacaagttg tcgaaaaatc ttcattagag aagaaatatg aggaagcaaa agcaaaagct
     2341 gatactgcca agaaagatta cgaaacggct aaaaagaaag cagaagacgc tcagaagaaa
     2401 tatgatgagg atcagaagaa aactgaagag aaagcgaaaa aagcagaaga agctgctaag
     2461 gaagtagatg atgctagttt agcggtgcaa aaagcttatg tggaacaaag aaaagcacaa
     2521 gaagaaaaaa ataaacattc aggaaatcca gagtacgcta aaaaattagc agaagcagat
     2581 aaaaaaataa ctgaggagac gactaaacta accgatgctc agacaaaatt tcaatctgtt
     2641 caagcaatgg tagttcctga gccagagcag ttggctgaga ctaagaaaaa agcagaagaa
     2701 gctaaagcag aagaaaaagt agctaagaga aaatatgatt atgcaactct aaaggtagta
     2761 ctagcgaaga aagaagtaga ggctaaggaa cttgaaattg aaaaacttca atatgaaatt
     2821 tctactttgg aacaagaagt tgctactgct caacatcaag tagataattt gaaaaaactt
     2881 cttgctggtg cggatcctga tgatggcaca gaagttatag aagctaaatt aaaaaaagga
     2941 gaagctgagc taaacgctaa acaagctgag ttagcaaaaa aacaaacaga acttgaaaaa
     3001 cttcttgaca gccttgatcc tgaaggtaag actcaggatg aattagataa agaagcagaa
     3061 gaagctgagt tggataaaaa agctgatgaa cttcaaaata aagttgctga tttagaaaaa
     3121 gaaattagta accttgaaat attacttgga ggggctgatc ctgaagatga tactgctgct
     3181 cttcaaaata aattagctac taaaaaagct gagttagcaa aaaaacaaac agaacttgaa
     3241 aaacttcttg acagccttga tcctgaaggt aagactcagg atgaattaga taaagaagct
     3301 gctgaagctg agttggataa aaaagttgaa gctcttcaaa acaaagttgc tgatttagaa
     3361 aaagaaatta gtaaccttga aatattactt ggaggggctg attctgaaga tgatactgct
     3421 gctcttcaaa ataaattagc tactaaaaaa gctgaattgg aaaaaactca aaaagaatta
     3481 gatgcagctc ttaatgagtt aggccctgat ggagatgaag aagaaactcc agcgccggct
     3541 cctcaaccag agcaaccagc tccagctcct gcaccaaaac cagagcaacc agctccagct
     3601 cctgcaccaa aaccagagca accagctcca gctcctgcac caaaaccaga gcaaccagct
     3661 cccgcaccaa aaacaggctg gaaacaagaa aacggtatgt ggtacttcta caatactgat
     3721 ggttcaatgg cgacaggatg gctccaaa
//
LOCUS       DAVKFH010000105         3377 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKFH010000105 DAVKFH010000000
VERSION     DAVKFH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.105"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..865
                     /locus_tag="VW006_002036"
     CDS             <1..865
                     /locus_tag="VW006_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEU9380821.1"
                     /translation="LKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSII
                     EDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKER
                     VLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQL
                     VPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKE
                     MDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQND
                     KKIVIPSLLEI"
     gene            1215..1919
                     /locus_tag="VW006_002037"
     CDS             1215..1919
                     /locus_tag="VW006_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEU9380822.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            1900..2829
                     /locus_tag="VW006_002038"
     CDS             1900..2829
                     /locus_tag="VW006_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU9380823.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            2826..>3377
                     /locus_tag="VW006_002039"
     CDS             2826..>3377
                     /locus_tag="VW006_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9380824.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFF
                     VTALLYVIQSLVPIANRYIIDTNF"
BASE COUNT         1206 a          414 c          569 g         1188 t
ORIGIN      
        1 attgaaagat aaatcatcat atactcaact gtacaataaa atgttatctg caattccgcc
       61 atctgtttct ttatctaaga ccacatttgg tttaactggg gatataggat atttcattgt
      121 tctatccatt atagaggatt acgatacaaa tactctcgca tgcttaaatt atataaagag
      181 tgttttaact gagcttgaaa agactgattt tgcaagtgtt tcaaacaagt ctgactatat
      241 taatggtctt ctacctttaa ttaatacact ggtaagatat tataggagag gaatagagaa
      301 ggaacgtgtt ctaaggctag tattaagttt aggtgatacg ctatataatg atgtaagcga
      361 gagagataat agtaattttg gatatagttt tggccatgga ctatcaggtg taatttttac
      421 attaaaaaat atttataaag tgacaagact taaaaaatat aaagaattaa ttttacaact
      481 agttcctagg gagaaaatca aaattcattc tttgaagtgg tgtagtggag aatttgggac
      541 attagttaat catccccaaa gtgttccaga gggaattgta gctagtttag ataatgatac
      601 attttgccat ggaagtatgg ccataataaa tttttacatc gactacatga aagaaatgga
      661 tactagtatg gaattggagt ctttattgtg tagtgttgta atgaataaat caaaagaagg
      721 agagtatcgt atcgtccaaa caagcaattt cccagatttt tctttgtata caggtgtgac
      781 tggtattgct tatacttttt taaggtatat cgcactgaat cagaatgaca aaaagatagt
      841 tatcccctca ttacttgaaa tctagcttaa tttatagttt tgtttctaaa atagaggtgt
      901 ccactcatta agcaatataa aaataccaag attgactagt ttaatatgta attctataaa
      961 atatttttaa tagtgtagct tttcgttgag ataataatat aataaagaaa agttctattc
     1021 taattgctaa acggcttgag gtatatgcta ctgttataga aatgatttta aactaaaatc
     1081 aaaattactc caaaaatagt attgattttg gtttaataca aaacatatgt aataatgtga
     1141 gtcaacaaaa gtctgttttt gtaccatact atcttgaaaa atctactaaa ttcaaattga
     1201 gaggatgaat taaaatgaat aaaatattta tatatgcagg agtgcgaaat cataactcta
     1261 agactttaga gtatactaag agactttcaa gtatcatttc tagtagaaat aatgttgata
     1321 tatctttcag gacacctttc aattcagagc tagaaatatc taattctgat tcagaagagt
     1381 tgtttaagaa aggaattgat aggcaaagta atgctgatga tggaggtgtt ataaaaaaag
     1441 agttattaga gtctgatatt ataattatct cttctcctgt atatttacaa aacgtatctg
     1501 tggacacaaa aaactttatt gaaaggattg gggggtggag ccatcttttt cgattagctg
     1561 gaaagtttgt tgtaacatta gatgttgctg agagtaacgg ctctgataat gtgtcagaat
     1621 atcttagaga tatattttca tatatgggag gacagatatt acaccaagtt tcaattacca
     1681 attcactcaa agatattgca gaggctcagt tgatggaggc aacttataaa attgaagatg
     1741 tacttgaagg gaaaattaag tacaaaacaa cggattatca agaaagagct taccagacat
     1801 taaaattaat tttagaaaat tatgattccg aacattttga gaagatgtat tgggagaaaa
     1861 aacgcctttt tgaagctaat agtttagagg aatggtatta tgttgaaaat attaaataac
     1921 tctgtcatat atttatgttt gattccgtta ttgttcctat tattgataat ttttccaaat
     1981 gattcattaa tctattattt tcgattgatt ttaatttcgt taatatcaat tacattacat
     2041 gaactaggtc attttttagt gggaagatgt ttatcttata aactagagat gcttgctact
     2101 ccctttttct tctatttcag aaaaaaaata tatttcaaat ttccagtgtt attagcattc
     2161 ggttattgtc aaatgagtaa tagaaacatt actaatgaaa aaaatagtga taggaatttg
     2221 gtgttctact tctttggggg aggtggagct aacctaattg tcgcaatatt agcacttttg
     2281 ggatttgtgc cgtacgcatc agaatttttt attttgaata taattctttt tctagttacg
     2341 gtatgtttac caattgatgg aacagatggc aatgctatta gagagatagt tttgtattca
     2401 aaagattcaa aaacatatca gagattcttt gcgaacagtc tctataataa tccttatata
     2461 acaatcgacg attttgcaaa attaacctct aaagaaaaat cttttttttc aaaatttgaa
     2521 aaatgtttga taaaccttta ctttgaagtg aaaggtgaag ggtcagcatt tacaattgct
     2581 aatcatgaag tttttgattc aaatattcag gaaaatatta tccatgagta ctataaatta
     2641 ctacataaat cttctgattg gattattcca cagaacttat ctttagaaga agttgttttt
     2701 accttgtcaa actatattta ctcgaaaaat aataaatatt tagaaaagat taagtatcta
     2761 aagaaattag tagattttag acaagaagaa attatcgact ttattttgaa caaggaggaa
     2821 gtattgtgaa aattccaatg atatatcaaa tggaaaattc agaatgtgga ttagcatgct
     2881 gtgccatgat attgaactat tttaaatatg agatttcttt aaatgaacta cgtgaaatct
     2941 acccatcatc cagatctgga tattctctcc tatctataag taaagtttta ggagatttta
     3001 atataagttc tcatgctttt aaagcttcgg taagagattt aaaaccgctc agtttcccac
     3061 tcatttgctt ctgggagagt tctcatttta ttattcttga aaaaattagt aaaaacaagt
     3121 tttatatttt agatcctgca aaaggcaggc agagaatgtc aataagtgaa tttgaaaggc
     3181 attattcaaa tatcatttta acatttaaaa agttagatag ctttatgtct cgtaaagata
     3241 ataagaagtc gcctgtttta aagtattttt ttaagtatag gaataagcta gggattttat
     3301 tttttgtaac agcattattg tatgtaatac aatcattagt acctatagct aatagataca
     3361 taattgacac gaatttc
//
LOCUS       DAVKFH010000106         3310 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKFH010000106 DAVKFH010000000
VERSION     DAVKFH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.106"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            467..1753
                     /locus_tag="VW006_002040"
     CDS             467..1753
                     /locus_tag="VW006_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9380825.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1768..2079
                     /locus_tag="VW006_002041"
     CDS             1768..2079
                     /locus_tag="VW006_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380826.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            2339..3067
                     /locus_tag="VW006_002042"
                     /pseudo
     CDS             2339..3067
                     /locus_tag="VW006_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT         1026 a          567 c          699 g         1018 t
ORIGIN      
        1 cttttttctt tttaaatatt gattgtttct aaaagttcta gtaatattgc tatttctcat
       61 ttttccaaat atgttattca acttattagc tacgtctcta ttcatatttg ggtataaaac
      121 aaaaaatcaa ttctaacagt tttatatcag aataataaat aaaaaagcga tattctagac
      181 tgtatcaatc cttttccatt ataggagcaa cagagtggga gtagtcatct aaggactaat
      241 ttatgtattt ttacgagtca gtatcttggg atactggttt ttactttttt agactttttg
      301 actacttgtt aaaactgggg taattttcga ctgtttaaca gttattatgc aaagtctaaa
      361 agattagaat tgtcaaaaca atccgtctag gcttgatttt atcctttatt tactataaaa
      421 tgagaaggaa aaatgtcaaa cttttatatt gcaaatagga gaaatcatga caaaaacatt
      481 aaaacgtcct gaggttttat cacctgcagg gactttagag aagctaaagg tagctgttca
      541 gtatggagca gatgctgtct ttatcggtgg tcaggcctat ggtcttcgta gccgtgcggg
      601 aaactttact ttcgaacaga tggaagaagg cgtgcagttt gcggccaagt atggtgccaa
      661 ggtctatgta gcggctaata tggttatgca cgaaggaaat gaagctggtg ctggtgagtg
      721 gttccgtaaa ctgcgtgata tcgggattgc agcagttatc gtatctgacc cagccttgat
      781 tatgattgca gcgactgaag caccaggcct tgaaatccac ctttctaccc aagccagtgc
      841 cactaactat gaaacccttg agttctggaa agagctaggc ttgactcgtg tcgttttagc
      901 gcgtgaggtt tcaatggaag aattagctga gatccgcaaa cgtacagatg ttgaaattga
      961 agcctttgtc catggagcta tgtgtatttc atactctgga cgttgtactc tttcaaacca
     1021 catgagtatg cgtgatgcca accgtggtgg atgttctcag tcatgccgtt ggaaatacga
     1081 cctttacgat atgccatttg ggaaagaacg taagagtttg cagggtgaga ttccagaaga
     1141 attttcaatg tcagccgttg acatgtctat gattgaccac attccagata tgattgaaaa
     1201 tggtgtggac agtctaaaaa tcgaaggacg tatgaagtct attcactacg tatcaacagt
     1261 aaccaactgc tacaaggcgg ctgtggatgc ctatcttgaa agtcctgaaa agtttgaagc
     1321 tatcaaacaa gacttggtgg acgagatgtg gaaggttgcc caacgtgaat tggcaacagg
     1381 tttctactac ggtataccat ctgaaaatga gcagttgttt ggtgctcgcc gtaaaattcc
     1441 tgagtacaag tttgtcgctg aagtggtttc ttatgatgat gcggtacaaa cagcaaccat
     1501 tcgtcaacga aacgtcatta acgaagggga ccaagttgag ttttatggtc caggtttccg
     1561 tcattttgaa acctatattg aagatttgca tgatgccaaa ggaaataaaa tcgaccgcgc
     1621 tccaaatcca atggaactat tgactattaa agtcccacaa cccgttcaat caggagacat
     1681 ggttcgtgca ttaaaagaag gactcatcaa tttttataag gaagatggaa ccagcgtcac
     1741 agttcgagct taagaaagga aaaggaaatg atagaggcac agggtttctt agtggataag
     1801 caaacaagat gcattcatta ccatagcaag ctggatatta ttgctttaca atgctatgat
     1861 tgtaaaaagt attatgcttg ttatcggtgt catgattcat tagaaaatca cccttttgag
     1921 ccttatccct tatctttgat acaggataag cctattttat gtggtgtttg tctaaaacta
     1981 ttaacatata agcaatataa agaaagctta agttgcctct tttgtttttc tcgctttaat
     2041 ccaggttgcc aaaatcataa ggaacgctat tttaaatagc aaatcatcta gttttgaagt
     2101 aggagaaaac tcaattccaa gagaaaatga agtaaatctt cccacaataa aacgcataat
     2161 atcaagattg ttcaatacct gatactatgc gtttttaaga ttttaaagac ttttttccta
     2221 gccccttttt actttatctg gtattttgac tacttgttaa aactggggta attttcgact
     2281 gtttaacagt tattatgcaa agtctaaaag gttagaattg tcaaaacaat ccgtctagag
     2341 tatgcgtgat gccaaccgtg gtggatgttc tcagtcatgc cgttggaagt acgaccttta
     2401 cgatatgcca tttgggaaag aacgtaagag tttgcagggt gagattccag aagaattttc
     2461 aatgtcagcc gttgatatgt ctatgattga ccatatctca gatatgattg aaaatggtgt
     2521 ggacagtcta aaaatcgaag gacgtatgga gtctattcac tatgtatcaa cagtaaccaa
     2581 ctgctacaag gcggctgtgg atgcctatct tgaaagtcct gaaaagtttg aagctatcaa
     2641 acaagacttg gtggacgaga tgtggaaggt tgcccaacgt gaactggcta caggatttta
     2701 ctatggtaca ccatctgaaa atgagcagtt gtttggtgct cgtcgtaaaa tccctgagta
     2761 caagtttgtc gctgaagtgg tttcttatga tgatgcgaca caaacagcaa ccattcgtca
     2821 acggaacgtc attaacgaag gggaccaagt tgagttttat ggtccaggtt tccgtcattt
     2881 tgaaacctat attgaagatt tgcatgatgc caaaggcaat aaaatcgacc gcgctccaaa
     2941 tccaatggaa ctattgacta ttaaagtccc acaacttgtt caagctggag acatggttcg
     3001 agctcttaaa gaggggctta tcaatcttta taaggaagat ggaaccagcg tcacagttcg
     3061 tgcttaatgt agttgtttag ttttaaaaaa ttatgcaaag ctccatatac aacacttaaa
     3121 cgagattaaa gaatggcgaa atcccttgat gcgcaagaga ttagctgtcc ttttttattt
     3181 tttaagtgat aaagtcggag cttaggtatt aaagcctatc aaattaaaca aagaagcgat
     3241 gtcttagata ttttgaaaaa aattaataag cagaaaactc tctattattt tgttgtagag
     3301 agttttttgt
//
LOCUS       DAVKFH010000107         3179 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKFH010000107 DAVKFH010000000
VERSION     DAVKFH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.107"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            20..751
                     /locus_tag="VW006_002043"
     CDS             20..751
                     /locus_tag="VW006_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380827.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            766..1917
                     /locus_tag="VW006_002044"
     CDS             766..1917
                     /locus_tag="VW006_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9380828.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(1969..2628)
                     /locus_tag="VW006_002045"
     CDS             complement(1969..2628)
                     /locus_tag="VW006_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9380829.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTCHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            2698..3144
                     /locus_tag="VW006_002046"
     CDS             2698..3144
                     /locus_tag="VW006_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9380830.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
BASE COUNT         1161 a          473 c          655 g          890 t
ORIGIN      
        1 atttggagga ggaagaatta tgttgaatca ggatctcttt gattcgcttg aggcacaaaa
       61 aattgtagat actttgatga aagggcaaaa agattatgta gatgaacgtc tagaaaaaag
      121 agagacaatg atagtgtcga atggttatgc atggacgcga cctaatcata ttgatactgc
      181 atttgcatca gcagatttgt ttgagtataa attacaatta gcaggacaga cttggggata
      241 tttagaattt gaaacaaata cagaaaaata tgggaaagta ttgttaatta taaagggtaa
      301 gaagcgactt acgaaccaat ttcctttggt acaaaaaaat aagagtggct acttatttga
      361 atatgctcaa atgaatacac tttatcttaa tcaacattct tcctacaaaa atgatgaaga
      421 tagtcattcc tttccaattc agatggagtt agtttctgat gaaatgattc aagaaattga
      481 acaagctacc aaaaattcga atatcgaaaa atttatgatt ttaacttatg aggcggactc
      541 agaaaacaat attatatctg tagatgttgt tatgcctgat gcacgaactg gtcagttaca
      601 cttgattcaa gatttgtctg agtatattca atcaagttcg taccatttcg aagaagctaa
      661 ataccaagat attcctaatt tttcagaatt atcggaaaca gaagattttg aaattattcc
      721 aagaatagaa aaacaagaag gtcaaaagta gtaaggagta tgtaaatgtt taatggtcgg
      781 gtattgaaag aattacggct gttaaatggt ttaagtagag cagaattagc tcagagaatt
      841 aatttaacgg aacaagccat ttggcagttt gagtccaacg aaacgaaacc taaattatca
      901 accaaaatgc atttggccaa ccaatttcat gttgatttaa cttattttga acaggaagaa
      961 gagagcattc gatttgattc ttctgtaatt gcctttagaa atgcagacct agcaacacgg
     1021 aaaacaatag atattcaaac tatgtattta cataaggtag atagtttgat tgattatttt
     1081 gaaagttttg taattatacc taatattaca attcatgacc taagtaatgt agtgagtgaa
     1141 tcttatcata agggagaatc cattgaggaa ttggctcttt atgccaggga aaaattaggt
     1201 atttcaaaag ataatcatga tttgctttat aaattagaac gttcaggcat ctatatcgtg
     1261 gaacgattaa ttaatggcca agctgatgct tatagcgcat ggtcaaaatt gggaagacct
     1321 tatattgtgt tgggaacgaa taaatcatct gtacgtcgaa attttgactt agctcatgag
     1381 ctaggacata ttcttttaca taaatataaa gatatgaatg aagatggcga tcgtttggag
     1441 caagaagcaa attattttgc atcatgtttt ttattgccaa aagaagagtt tttagtcaaa
     1501 tttgaagaga gggttggcaa gcgtgtcagc aatcctgata gttatatttt attgaagtcg
     1561 gatttgaatg tttcgataca ggctttagag tatcgagctt ttaagttagg attattgact
     1621 ccaaagcaac attcttactt ttatcgtcaa attgcgcaaa aaggttacaa aatgattgaa
     1681 cctttggatg atcaaatttt tgttaaaaaa ccaagcaaag taaagagtat tctggacgtc
     1741 gttttgagta atcatctagt cagtctagcg actataatgt ctaaacaaag tattcgttta
     1801 cagtttataa gcgaaatatt ttcagtcgaa atgaaatttt ttgatcagta tcaagaagat
     1861 agaagaacag atcgatttga taacatcatc cctttgtaca aaagaaataa tttataattt
     1921 aactgtgaaa taatttcatg aaggacatat ggcagaaatt ggattttatt agacaagcag
     1981 tagcttacca aaaataatca aacaagtagc aagtaagagg aaaggcacga aaggaagtct
     2041 ttcctttttc ttttgcagga gaaaggccag gatacccgtc gcagaagcga actgaatcaa
     2101 gatcagtaac tccgttacgc taaagacgag agcacaagaa gctaaaaaaa ggaaatcccc
     2161 tgcacccatg cggatatcga taaaatgagc caaaattcca agtatgagga aggagaccat
     2221 gaccagattc cagccagagg aagctattag gattaggtgg caagtcatcc agaccagtaa
     2281 gggatattcc tgatggtgaa agtcgtagat acccaaggtc aaaccagcgg tgattaggac
     2341 gacttgcccc aaggaaagca atttccaaga gtaaagcaga aagaggagtc ctaagactaa
     2401 ttcaaagagg gcataccaga caggatagcg aactttgcag tagcgacagc gaaagcgatt
     2461 gaagacctgt gagagaatcg gaatcaaatc taagggacgc aagcgagtct gacaggaatc
     2521 gcagtgactg gctgaactga taatggattg ctctggaaaa cggtcaatga ccaaaccaag
     2581 aaaggaagcg agaatgctcc cgacgagaaa aaaataaaaa tcaatcatac ttatctattc
     2641 gtaaaaaatg ggagaaatag tataatggag aaacatagat aagatggtga ggaaaagatg
     2701 acaattcgtt ttgaagaaaa ggtgagtata gaaaacgctc aatttgtatg ccagtggtcc
     2761 aactccctag gcaaatcctt tcaagaacaa tggatgggac caaggattcc ttttctactg
     2821 acccttcaag ccttggaagg agtcttttca atctttgatg aacaagagtt tgtgggattt
     2881 atccagaaaa tcaggctaga agacagcaat cttcatatcg ggagattttt tatcaacccc
     2941 cagaaacagg agcaaggctt aggtagccag gctttaagga aatttgttag tttggccttt
     3001 gaaaatgaag atatagatag tatttctcta aatgtcttcg aggcaaatca aagagctcag
     3061 aatctttacc aaaaagaagg atttgaaatc gttcaaatgg ttgaagcacc tgtacgaaaa
     3121 tatagtatat tgaaactaga atagtacacc tctacttcta aaacattgtt agaaatcga
//
LOCUS       DAVKFH010000108         3134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKFH010000108 DAVKFH010000000
VERSION     DAVKFH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.108"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..162
                     /locus_tag="VW006_002047"
     CDS             <1..162
                     /locus_tag="VW006_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9380831.1"
                     /translation="SFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTLITGK
                     NGTGKSTLL"
     gene            complement(528..1838)
                     /locus_tag="VW006_002048"
     CDS             complement(528..1838)
                     /locus_tag="VW006_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9380832.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2075..2947
                     /locus_tag="VW006_002049"
     CDS             2075..2947
                     /locus_tag="VW006_002049"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9380833.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          960 a          525 c          711 g          938 t
ORIGIN      
        1 tcttttgcta ttagttcaaa aggagaaaaa atgaaagtag aaaatatttc gtatagggtg
       61 gatcatcgta tattgtttga taatatttct tttgatactt cgagttcagg cgtgacatta
      121 attactggta aaaatggtac aggaaagtca actttactat agtagattga aactagaata
      181 gtacacatct acttctaaaa tattgttaga aatcgatttg actatcctga tctatttgtc
      241 ctgttcttat ttcatttcac tatatctcaa attgagtatg acgaagtgcg ctcccatgtc
      301 ctgggaacgc actttcttca tatttttcat attcttgaat ccatcgataa agactattgg
      361 gatgaatttt taaagttgaa ctaatcattt ttacaggatg agatttacag cagagagttt
      421 gaaggcttta tcaaaggttt ttcttggcat aatgactttt cctcgtttcc acttaatttt
      481 gtgtctactt tattatacca agtccaccct taagttagat aataaatcta acttaaggaa
      541 gctagaagga tgagaatcca ggtggtcaag agtcccaaac ttaagctgat ggggacaccc
      601 agaataattt gctttttgaa ggcaaggcca cgttcctcta tattgggaag tgagagttga
      661 atgagagaac cagctgatga aaagggtgag atattagtag atagagcgcc aataacggtg
      721 gctgttgtga gtaagtgaat atcaatctga ggattttgag cactgatgat agcaatgatg
      781 ggaaagaggg ctggagctac aacggatagg gtggaactaa agagtgacat cactccggct
      841 atcacacaaa agaacagagg taaccagaaa tgaggaatgg ttgttgtcat gaggtgccct
      901 atcagtgtga ctaaacctga cttgaccgct agagacatta gtaagctcat gccgcagagc
      961 atgataattg tagcccaggg aaccttagct aaaatggctt cttgcttccc taatttgagc
     1021 cttaaggcga ggcagaccat gagtattgag acaaagccaa tatcaaatgt tttttgataa
     1081 gtagctatcc aggcgatgtt tgggaaaatg agatgcaaca agggaaaaag ccaaaccaaa
     1141 accatgctgc tgatcatgag caaggtggtt tgtctttgaa ccttgctgag gagtggtggt
     1201 tggtcaatag tcaaggatga gtttgttctt cccttactat agtgactgta acaggataat
     1261 aaaagcaaga cgatgagtgg gtagataatg ctgacgataa agatatgatt gccaagtgaa
     1321 aaagcttgct cttcccatcc catttgctta aacaggcctt gaaagacaat gcctgagcta
     1381 ctggttatca aattagcccc tcctgaagct ccccaattga cggcttgagc tccaatcaaa
     1441 gggtgtttgt ccgctttttg acagagggta atcgctagag gacagcaaac ggccatagta
     1501 gtgaaaaatc cagcacctaa agcagacaaa agggttgcca tcaggtataa aatcatgtag
     1561 agggcgttag ggtgggtgcg tgtgcggtag agaatgtgtt gagccaaaac atcaagagta
     1621 ccgttagttg ttgcaacgtt ataaaagaga gagacgctaa aaatggtaaa aaagagtgag
     1681 gttggccaaa aatgaagaag ttctttgggg cttaatccca tgagagtggt tgcgatgagg
     1741 taagaaaaag caatagccag caggccaata ttgattttgg tgcggtaacc aattccaatg
     1801 gctagagcaa tggcgctaat cattattaaa tgaatcattg aattgtcctt tcagttagaa
     1861 tatggaaata ggttggcaat tttggcgctc aaaagaccac ctgtttccta ttggtacttg
     1921 actattatac acaaaaaaaa tacaattgtc ttgacaattg tattgacact tgttagaatt
     1981 aaaaatgagg tattcaggct tgtgatttcc tatggaagtt aatagtaatt gcctctagtg
     2041 cttacaagtg atattaaaaa tagaggactt agtgatgtca atcatttcaa ctgatttaac
     2101 cccttttcaa atagatgata cattgaaagc agccttgcga gaagatgttc attccgaaga
     2161 ttacagtacc aatgccattt ttgatcatca tggccaagcc aaggtgtcgc tttttgccaa
     2221 ggaagctggt gttttagcgg gactaaccgt ttttcaaagg gtttttaccc tatttgatgc
     2281 cgaggtgacc ttccagaatc ctcatcaatt taaggatggg gatcgtttga ctagtggcga
     2341 tttggtttta gaaatcatag gctcggtgag aagtctctta acatgtgaac gcgttgcctt
     2401 gaatttttta caacatttat cagggattgc ttcgatgaca gctgcttatg tagaagcctt
     2461 aggcgatgat tgcattaagg tatttgatac tcgaaaaact actcctaatt tacgtctttt
     2521 tgagaaatat gccgtgagag ttggcggtgg ctataatcat cgctttaatt tatcagatgc
     2581 tatcatgcta aaagacaatc acattgcggc agtaggtagt gttcagaagg caattgctca
     2641 agcgcgtgcc tatgcccctt ttgtgaaaat ggtcgaggtg gaagtggaaa gccttgctgc
     2701 tgccgaagaa gctgcggcgg cgggtgctga tattatcatg ttggataata tgtcattgga
     2761 acagattgaa caggccatta ccctaattgc aggacgttct cggattgaat gttctggaaa
     2821 tattgatatg accactatta gccgttttcg tggtttagcg attgattacg tctccagtgg
     2881 tagtttaacc catagtgcta agagtcttga tttttccatg aagggtttaa cctaccttga
     2941 tgtctaagtt gtaaaataaa ctaacttttt aaaggatgtc tttcctctag aacgagtttt
     3001 atgtcagata gtttaaacgc ctcttcaaat atagtaaaat gaaccaaaaa tagtacacaa
     3061 tgtggtataa tcttcttatg gcatattcaa tagattttcg taaaaaagtt ctctcttatt
     3121 gtgagcgaac aggt
//
LOCUS       DAVKFH010000109         3097 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKFH010000109 DAVKFH010000000
VERSION     DAVKFH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.109"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(128..661)
                     /locus_tag="VW006_002050"
     CDS             complement(128..661)
                     /locus_tag="VW006_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9380834.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(750..1526)
                     /gene="recX"
                     /locus_tag="VW006_002051"
     CDS             complement(750..1526)
                     /gene="recX"
                     /locus_tag="VW006_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9380835.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1564..2919
                     /gene="rlmD"
                     /locus_tag="VW006_002052"
     CDS             1564..2919
                     /gene="rlmD"
                     /locus_tag="VW006_002052"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9380836.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            2981..3052
                     /locus_tag="VW006_002053"
     tRNA            2981..3052
                     /locus_tag="VW006_002053"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3014..3016,aa:Glu,seq:ttc)
BASE COUNT          982 a          556 c          578 g          981 t
ORIGIN      
        1 ataaatctct ttctagtaat gtgttgtaac tctgctataa tggatttatt cctttttgta
       61 tttacacaat ttattttata ctaccaaaaa aacaaggtca ggattttgtt cctgaccttt
      121 gacaacttta ccgattcttt agttctacat agcgcttgta ccaaatgttt acataggctt
      181 ctgagaaagg accacgtcca ttgttaatcc aatcaacaag aattttgaca tgttctttta
      241 aaatatagtc caagtcatca gaataattca ttttgcgttt gtgacgctcg tattcttcaa
      301 cgtccaagag acgtttttcc ccatctgtaa aaattttaac atccaaatcg taatcaatat
      361 acttcagtgc ttcttcatcc agatagtagg ggctagccat attgcaatag taagaagttc
      421 cattatcacg aatcatggca atgatattaa accaatattt cttgtgaaag taaacaatag
      481 ccggttctcg agtgacccaa cgacgaccat cactttcggt aacaagtgta tgatcgttga
      541 caccaataat ggcgttttct gttgttttta gtaccatggt gtcccgccaa gttcggtgga
      601 gactcccatc atgcttataa ctttgaattg taataaagtc gccttctttt ggaagcttca
      661 taactaacca actttctaca atttataagt ttatcattta ctattgtacc ataaaattac
      721 ctaaaatctg tgaatttcac ttggaaatat taaagatatt ctctaagagc gcttgctata
      781 tccgaaaaat cgtagccctt tcgtgctaaa acttgagtta aacgctgctt cagttcgtat
      841 ccttcatact ttcgagcata cttagtatat tgcttatcaa gttccttgaa gatgagttct
      901 tgagtcgttt cttggtcaac ttggctatct aagtcgtcaa aggcaatttt agcatcagaa
      961 taagagaagc ccttgttagt caagttctgg ataatcttat cttgcagggc acgagctgga
     1021 agttttccct catatttttt caatagttta ttggctacac gttgagcaac ttcagaaaaa
     1081 tcaaattctt tcaagttctc ttctatagta gattttgaaa tccctttttg agatagtttc
     1141 tgagtcagca cataaggacc cttgtctcct gaaagttgat tggtattgat gatagcataa
     1201 gcgtactgac catcattaat ccacttatct tctttaagat tagcaataac ttgagaaacg
     1261 atgtttttat caatatcgta ttttttcaga tattctctga cttctttttc agtgcgtgct
     1321 ttaaaggata agtggtagag agcgagattc ttaccataag aaaattgagc aaagtcttga
     1381 atctctttca attcctcttc gcttatcacc ttatctctcg ataacataaa acgaacaatt
     1441 gtatcttcgg tgatatagca tttgtcgcca ttatcaagct ccatcaggta gagtcttttt
     1501 ttcttttcaa gttttgtgat tttcatagct ctattataac tcaaaatgtg ataagatagg
     1561 ggtatgaatc tgaaagtgaa acaaaaaata ccattaaaaa tcaagcgcat gggaattaac
     1621 ggtgagggaa tcggctttta ccaaaaaaca ttagtctttg taccaggagc tctcaaaggc
     1681 gaagatatct attgtcagat tacttctatt agacgcaact ttgttgaagc aaaattactg
     1741 aaggtcaaca agaagtctaa atttcgaatt gtgccatctt gtactattta taatgaatgc
     1801 ggaggctgcc aaatcatgca cctgcattat gataagcagc tggagttcaa gacggactta
     1861 cttcatcaag cgctgaaaaa atttgctcct gcaggatatg aaaattatga aattcgtcca
     1921 actattggaa tgcaggaacc aaaatattac agagctaagt tacaatttca gactcgaaaa
     1981 tttaaaaatc aggtcaaggc gggcttatat gcacaaaact ctcactattt agtagagttg
     2041 aaagactgcc tggtacaaga taaggaaacc caagtgattg ctaatcgctt agcagaatta
     2101 cttacttatc accagattcc aatcacggat gagagaaaag ttctaggtgt ccgtactatt
     2161 atggtccgac gcgcgagaaa gaccggacag gttcagatta ttattgttac aaaccgccag
     2221 cttaatttaa ctcaattggt aaaagagttg gttaaagatt tcccagaagt tgtgacagta
     2281 gctgttaata caaatacagc taaaaccagt gagatatatg gtgaaaagac agagattatc
     2341 tgggggcaag agagtattca agaaggtgta ctcaattatg aattttcact atcccctcga
     2401 gctttttatc aactaaatcc tgagcaaaca gaagtcctct atagcgaagc agtaaaagcg
     2461 ctggatgttg ataaagaaga ccatttgatt gacgcttatt gtggagttgg aacgattgga
     2521 tttgcctttg caaagaaagt taaaacactc agaggtatgg atattattcc agaagctatt
     2581 gaagatgcca agcgaaatgc taaaagaatg ggatttgaca atactcatta tgaagctgga
     2641 acggcagaag agattattcc tcgttggtac aaggaaggct accgagcaga tgctttgatt
     2701 gttgacccac cacgtacagg tctggatgat aagttattag atactattct tacttatgta
     2761 ccagaaaaaa tggtttatat ttcttgtaat gtttcgacct tggctcgtga tttggtacgc
     2821 ttagtagaag tctatgatct tcattatatc cagtcggtcg atatgttccc acatacagct
     2881 cgaactgaag ctgttgtaaa attaataaaa aaagtttaaa aaagtagttg acaaagtttg
     2941 aaaaggctgt ataatagtaa gagttgaaaa taacaactca ggtccgttgg tcaaggggtt
     3001 aagacaccgc cttttcacgg cggtaacacg ggttcgaatc ccgtacggac tatggtatgt
     3061 tgcgtcagga ccacttgatg aaaaaaagtt taaaaaa
//
LOCUS       DAVKFH010000110         2812 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKFH010000110 DAVKFH010000000
VERSION     DAVKFH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.110"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..339)
                     /locus_tag="VW006_002054"
     CDS             complement(<1..339)
                     /locus_tag="VW006_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380837.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYA"
     gene            complement(397..1110)
                     /locus_tag="VW006_002055"
     CDS             complement(397..1110)
                     /locus_tag="VW006_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9380838.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1568..1948)
                     /locus_tag="VW006_002056"
                     /pseudo
     CDS             complement(1568..1948)
                     /locus_tag="VW006_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
     gene            complement(2365..>2812)
                     /locus_tag="VW006_002057"
     CDS             complement(2365..>2812)
                     /locus_tag="VW006_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9380839.1"
                     /translation="RNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHPESDE
                     KENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEK
                     VTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESK"
BASE COUNT          874 a          556 c          437 g          945 t
ORIGIN      
        1 ggcatatttt gctacatgat ctaggtatat atcaaaaaag tcatgaggaa aatctcccat
       61 acagccatta cgattcatat tgaggctata ttttcgatta gtaagataat tcggaaatat
      121 atttcctatt actctaagat tatctacttg tgaatccctt ctcccaatat aatccccttc
      181 tttgactaat gatggagatt tccaagtaaa aattaaatcg ccagagaaat ttttcttatc
      241 aaaaaactta aaaagagatt gataaatact atttgctaat tgacaagtgt cagatgtaaa
      301 ttcataatta acagcttcat taaaatcaac ttcagacatt tcaatgtact cttcaatagt
      361 aatattagct tttttgtagt cattagctcg tctcctttaa aatgtgatct tttcaagaat
      421 atttttatct aaacaatcca gcaagtcttg gtaagaatag acttcgtaag tcggctgggc
      481 ttgtgtgtga ttttcgaggt gatgaggatt ataccagata gtgtcaatcc ccgcattatt
      541 gccaccttga atgtcggcgg ttagagaatc tccaatcatc agcgtctttt ctttactaaa
      601 tccagcaatt tgctggccaa tcttttcata aaaaagagca tccggctttt gagtttgcaa
      661 ctgttctgag ataaagactt gattgaaata aggtgctaga ccagattgag ccaaacgtcc
      721 tgtctgaatg gcagtaatgc catttgtcgc agcatacaag tcataatcac gctcaatgag
      781 gctgtccaag agatcatgag cgcccgatag tgtttgtccc tgctgagcga gataaaattg
      841 gtaacgctgg gcaagaaaac taccgtcttt ttcctgtcca aaatgagaaa ataaacgaga
      901 aaagcgcgtg ttaaccagct cttgtttact gattttcttc agctccaagt ctttccagag
      961 agccttgttc ataggaacgt aataatcttt ataagcctga atatccgcaa ctccttcttc
     1021 ttttagaagt tgggtcaaag ccacatcctc agcagcatca aaatcaagaa gagtgtggtc
     1081 gaggtcgaag agtagaaatt tgtagaacaa tttgaggttt tcctttctga aaattcatta
     1141 agaacattat atcataaagc acctcataca attaactaat ttaatcactt aaaaaaattc
     1201 gaacactttc tatacaactg acagctcaaa tctttcagaa tagaacaata ctaactatcg
     1261 agcaccccgt attcataaat atatgtgtaa ttctagacct agaattccta taaactaaat
     1321 gctttcatac tcttccaagt aattgattgc cttaaatttt aatttttgaa ggtttctaaa
     1381 gctagaatag ccccatcaca atcagttttg attgattcac aatttagaaa cactatagtt
     1441 tcactcctgt taaaataaaa aggaactgca taaagcaatc cctttctgat tttgaaatca
     1501 tttacttaac attttatagt tgagataatc aatagcttac ctataaaaag agttatagta
     1561 aaattcctta tttattgatt tcaagctccg ctaactgtgt ttgaataact gacagttctg
     1621 caccagcctg aagaagagca gctgcattat aggcaccttc tacaattgga accctgttga
     1681 tgatgatact tttatcactg aaatcagtca ccatttttaa gttcatttta gcagaaccta
     1741 ggtcaaaaaa ggcaagtaaa gtatctgctg tattttcgga aacaacccta tctacttaat
     1801 caaaactcgt tccaattcct ccgccctcgg ttcctcctac ataagtaatc ggaacatctt
     1861 tagctacttt actaatcagt tcaacaacac cttctgcaat gtgtttggaa tgtgaaacga
     1921 taacaagacc aataccaata ctttccatca aaccacccca gtttctaaaa tagcagtaaa
     1981 gagtaatcct gatgagaatg atccaggatc aatatgtcca agaaaccata tgctcctaag
     2041 acaagagcca acagactggt catcaataat agtattgttc tttttttcat cattactcct
     2101 taactagtgt ttaactgatt aattagccag taaatagttt atctttattt acactatctg
     2161 ttaagatata gtaaaataag aacaggacag tcaaatcgat ttctaacaat gttttagaag
     2221 tagaggtata ctattctagt ttcaatctac tatattttgc acattttcat gaaaaaaatg
     2281 agaactagaa ctcacattct gctctcattt ttcgttttcc cgttctccta tcctgttttt
     2341 aggagttaga aaatgctgct acctttactt actctccttt aataaagcca agagactatc
     2401 tacttctgct gaaatagtgt tattatcttt cgttccgaga agaagactac tttttagacc
     2461 agtcaatgtc tccacagcat tttgtctaat actagaatct gttacttttt ctagcaaggc
     2521 ctccgcatct gctatcttag cgttaataac agaatgctct acttgaggaa tttcagcctc
     2581 atctgtagta tcttcagctt cttcctctgt ctcttccgta tcagtgcttg gtttataaag
     2641 attatctgct gaaggattta aaccagcgtg attctctttt tcatcagatt cagggtgagt
     2701 tggctcactt acttcatcat gttccttatc ttcctcaggt ttttctgtct gaggtttctc
     2761 ctcgtttggt ttttccgttt gattggtatc agcttgacca tttttgtttc tt
//
LOCUS       DAVKFH010000111         2797 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKFH010000111 DAVKFH010000000
VERSION     DAVKFH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.111"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..802
                     /locus_tag="VW006_002058"
     CDS             <1..802
                     /locus_tag="VW006_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9380840.1"
                     /translation="RDYWLEYPWGEKDISLIKLLNRIYRPFVKKSLRHLDIVTAPSAF
                     TLNNFINKGFINPSSQKCMVIENAVIFSKGKLENIIKQKQETKRNPEKTNFLFVGSIL
                     EIKGVFNLVKAFKKLTSPEISLKIVGKGKDLEKLQKEIESDPRIQYLGFQDTETLYKT
                     YQNSDVLVVPSAWDEPFGRVVIEGNANGLPVITSDRGGLPEIVQTVGGGEIFTDESGN
                     TLYSLLKKFVEMENYNVYYQCILENIDIYSIEKQSEKFTNLYLDKEIE"
     gene            836..2068
                     /locus_tag="VW006_002059"
     CDS             836..2068
                     /locus_tag="VW006_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU9380841.1"
                     /translation="MKIAVAGTGYVGLSIAVLLAQHHEIKAVDIVAEKVELINQKKSP
                     IQDDYIEKYLVEKDLNLEATMDAKYAYEDAEFVVVAAPTNYDSKKNFFDTSAVEAVIQ
                     QVKKINPKAVIIIKSTIPVGFTESVQKKYQYKNILFSPEFLRESKALYDNLYPSRIIV
                     GTDLEDTYLKDKANIFASLLKEGAIKEDIDTLFMGVTEAEAVKLFANTYLALRVSYFN
                     ELDTYAEVKGLDAKAIIDGIGLDPRIGNHYNNPSFGYGGYCLPKDSKQLLANYFDVPQ
                     NMMTATVESNRTRKDFIAQRILEKAGVSETDSLDAFKNIQDIVIGIYRLTMKSNSDNF
                     RHSSIQGVMKRLKAKGVTVIIYEPTLKDGETFFGNKVVNNLDKFKEASNVIVANRFEP
                     SLEDVSNKVYSRDIFKRD"
     gene            2287..2797
                     /locus_tag="VW006_002060"
                     /pseudo
     CDS             2287..2797
                     /locus_tag="VW006_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1009 a          396 c          533 g          859 t
ORIGIN      
        1 gcgagactat tggctagaat atccatgggg tgagaaagat atttctctta tcaaacttct
       61 aaatagaata tatagacctt ttgttaagaa aagtttaaga cacttagaca tagtaacagc
      121 accatcagca tttactttga ataattttat caataaagga tttattaacc catcttctca
      181 aaaatgtatg gtcattgaaa atgctgttat attttcaaag ggtaagttgg aaaatattat
      241 caaacaaaag caagaaacaa aaagaaatcc agaaaaaacg aattttcttt ttgtaggttc
      301 tatacttgaa ataaagggcg tttttaactt agtaaaggcc tttaaaaaac taactagtcc
      361 ggaaatttct ttgaaaattg taggaaaagg aaaagattta gaaaaacttc aaaaagaaat
      421 tgagagtgat cctagaatac aatatttagg ctttcaagat acagaaaccc tctataaaac
      481 ttatcaaaat tcggatgtat tggtcgtccc atctgcttgg gatgaaccgt ttggtagagt
      541 tgtaattgaa ggaaatgcaa atggccttcc tgtcatcact tcagatagag gtggattacc
      601 tgaaattgtg caaacagtag gaggaggaga aatttttaca gatgaatcgg gaaatacatt
      661 atatagtctt ttaaagaaat ttgtagaaat ggaaaactac aatgtctatt accaatgcat
      721 tcttgagaat attgatattt attctatcga aaaacaaagt gaaaagttca ctaatctgta
      781 tttagataaa gaaatcgaat aataagttgt ttttgtagca gtaacggagg taattatgaa
      841 aatagcggta gcaggaacag gttatgtagg attatcaatt gctgtgttgt tggcacaaca
      901 tcacgaaata aaagcagttg atattgttgc tgaaaaagtt gaattaatca atcaaaaaaa
      961 gtctccaatt caggatgatt atattgaaaa atatttagtg gaaaaagatt taaatttgga
     1021 agcaactatg gatgctaagt atgcttatga agatgcagaa tttgttgttg ttgcagcacc
     1081 aacaaattat gattcgaaaa agaacttttt tgatacatca gcagtagagg cggtcattca
     1141 acaagtaaaa aaaattaatc ccaaagcggt aataattatt aaatctacaa tccctgttgg
     1201 attcacagag tccgttcaaa agaaatatca gtataaaaat atcttattta gtccagaatt
     1261 tttacgcgaa tctaaagcat tatacgataa cctgtatcca agtcgcatca ttgttggaac
     1321 tgacttagaa gatacttatt taaaagacaa agctaatata tttgcttccc tactaaaaga
     1381 gggggctatt aaagaagaca tagatacact tttcatggga gtaacggaag ctgaggcagt
     1441 taaattattt gccaatacct accttgcgtt gcgtgtttct tactttaatg aattggatac
     1501 ttatgcagag gtgaaaggtt tggatgcaaa agccattatt gatggtattg gcctagatcc
     1561 acgtatagga aatcattata acaatccatc ctttggctac ggtgggtatt gtttacctaa
     1621 agatagtaag cagttattag caaattactt tgatgtacca caaaatatga tgacagcaac
     1681 tgttgaaagc aatcgaacgc gtaaggattt tatcgctcaa cggattttag aaaaagctgg
     1741 agtatctgag actgatagtt tagatgcgtt taagaacata caagatattg ttataggtat
     1801 ttatcgttta acaatgaaaa gtaattcgga taacttccgt cactcctcta ttcaaggcgt
     1861 tatgaaacga ttgaaagcta agggagttac ggttatcatc tatgagccaa ctttgaagga
     1921 cggggaaaca ttctttggta ataaagtggt aaataatcta gataaattta aagaagctag
     1981 taatgttatt gtagccaatc gttttgaacc ttcactagag gatgtgtcta ataaagttta
     2041 tagtcgagat atatttaagc gtgactaata aatacgtcca cgtgacgtaa tatctattaa
     2101 atgaataaaa tattaataaa catggcatcg atatcttttt tggacttgaa ttgtcaaatt
     2161 aagtgcacat ttttttcata gaaaacttca taaggtgttt tccggttaag acattttcgt
     2221 ggtctattat ctaagttggt ttatataata gacttcctgc gaaacaaaat atggtataat
     2281 agttctatga attatgaagc aagtaaacaa ctaaccgatg tacgatttaa gcgtcttgtt
     2341 ggtgttcaac gcacgacttt tgaagagatg ttagctgtat taaaaacagc ttatcaactt
     2401 aaacacgcaa aaggtggacg aaaacctaaa ttaagcctag aagactttct tatggcgact
     2461 cttcaataag tgcgagaata ccgcacttat gaacaaattg cggctgattt tggtattcac
     2521 gaaagcaact taatccgtcg gagctaatgg gttgaagtaa ctcttgttca aaggtggtgt
     2581 tacgatttca agaactcctc tcagttctga ggacacggta atgattgatg cgacggaagt
     2641 aaaaatcaat cgccctaaaa aaagaattag cgaattattc tggtaaaaag aaatttcacg
     2701 ctatgaagac tcaagcgatt gtcacaagtc aagggagaat tgtttctttg gatatcactg
     2761 tgaactattg tcatgatatg aagttgttca aaatgag
//
LOCUS       DAVKFH010000112         2535 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKFH010000112 DAVKFH010000000
VERSION     DAVKFH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.112"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..559
                     /locus_tag="VW006_002061"
     CDS             <1..559
                     /locus_tag="VW006_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9380842.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VW006_002062"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VW006_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9380843.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2029
                     /locus_tag="VW006_002063"
     CDS             1424..2029
                     /locus_tag="VW006_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9380844.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1977..>2535)
                     /locus_tag="VW006_002064"
     CDS             complement(1977..>2535)
                     /locus_tag="VW006_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9380845.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          868 a          364 c          435 g          868 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaacca tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgaac aagtttattt
     1681 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacacta agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tggaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 ccttttatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2161 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
     2221 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2281 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2341 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
     2401 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2461 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2521 gtttcgtctt tgttc
//
LOCUS       DAVKFH010000113         2504 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKFH010000113 DAVKFH010000000
VERSION     DAVKFH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.113"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="VW006_002065"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="VW006_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9380846.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1449..2504)
                     /locus_tag="VW006_002066"
                     /pseudo
     CDS             complement(1449..2504)
                     /locus_tag="VW006_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          769 a          490 c          530 g          715 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacatg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggttct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat ttcgtccttt ctttttgatg ttcagggcga
     1501 tgaaaatccg ttttttgaag ttttcaaagt tccgaaaacc aaaggcattg cgcttgataa
     1561 gtttgatgag attattggtc gcttccaatt tggcgtttga atagggtagt tgaagggcgt
     1621 tgacgatttt ctctttgtcc tttagaaagg ttttaaagac agtctgaaaa agaggatgaa
     1681 cctgcttaag attgtcctca atgagtccga aaaatttctc cggttcctta ttctgaaagt
     1741 gaaacagcaa gagttgatag agctgatagt ggtgtttcaa gtcttccgaa taactcaaaa
     1801 gcttgtttaa aatctcttta ttggttaagt gcatacgaaa agtaggacga taaaatcgct
     1861 tatcactcag tttacgacta tcctgttgta tgagcttcca gtagcgcttg atagccttgt
     1921 attcatgaga ctttcgatga aactgattca tgatttggac acgcacacga ctcatagtac
     1981 ggctaagatg ttgtacaatg tgaaagcgat caagaacgat tttagcattc gggagtgaaa
     2041 cagtctggga gactgtttca gcctgagcct agaaatttga aagcgaagct gtttagctaa
     2101 gtcatagtaa ggactaaaca tatccatagt aataattttg acgcgacatc ggacggctct
     2161 atcatattta agaaagtgat ctcggatgac agcttgtgtt ctaccctcaa gaacagtgat
     2221 gatattgaga ttgttaaaat cttgcgcaat gaagctcatc tccatctccc gattgaaaca
     2281 gtcactcccc ggactgtttc aacgtcccag gacataatct caggaagacg cgaaaaatca
     2341 tgctcaaagt gagaatcatt gagcttgcga atgacagttg aagttgagat ggaaagctga
     2401 tgggcaatat cagtcataga aatcttttca attaactttt gagcaatctt ttggttgatg
     2461 atacgaggga tttggtgatt cttcttgacg atagaagttt cagc
//
LOCUS       DAVKFH010000114         2365 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKFH010000114 DAVKFH010000000
VERSION     DAVKFH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.114"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            15..932
                     /gene="adcAII"
                     /locus_tag="VW006_002067"
     CDS             15..932
                     /gene="adcAII"
                     /locus_tag="VW006_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9380847.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            940..>2365
                     /gene="phtD"
                     /locus_tag="VW006_002068"
     CDS             940..>2365
                     /gene="phtD"
                     /locus_tag="VW006_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9380848.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPVQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVRDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEKNGVSRYIPAKNLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFE"
BASE COUNT          788 a          455 c          500 g          622 t
ORIGIN      
        1 ttaaggagtt gttcatgaag aaacaaaatt tatttttagt cctgttaagt gtctttcttt
       61 tgtgcttggg ggcttgtggt caaaaggaaa gtcagacagg aaaggggatg aaaattgtga
      121 ccagttttta tcctatctac gctatggtta aggaagtatc tggtgacttg aatgatgttc
      181 ggatgattca gtcaagtagt ggtattcact cctttgaacc ttcggcaaat gatatcgcag
      241 ccatctatga tgcagatgtc tttgtttacc attctcatac actcgaatct tgggcaggaa
      301 gtctggatcc aaatctaaaa aaatccaaag tgaaggtctt agaggcttct gagggaatga
      361 ccttggaacg tgtccctgga ctagaggatg tggaagcagg ggatggagtt gatgaaaaaa
      421 cgctctatga ccctcacaca tggctagatc ctgaaaaagc tggagaagaa gcccaaatta
      481 tcgctgataa actttcagag gtggatagtg agcataaaga gacttatcaa aaaaatgcgc
      541 aagcctttat caaaaaagct caggaattga ctaagaaatt ccaaccaaaa tttgaaaaag
      601 cgactcagaa aacatttgta acacaacata cagccttttc ttatctagcg aagagatttg
      661 ggcttaatca acttggtatt gcaggtatct ctcctgaaca agaaccaagt ccacgacaac
      721 taacagaaat tcaggaattt gttaagacct ataaggttaa aacgattttt acagaaagta
      781 acgcttcttc aaaagtagct gaaactcttg tcaaatcaac aggtgtgggt cttaaaactc
      841 tgaatccttt agagtcagac ccacaaaatg acaagaccta tttagaaaat cttgaagaaa
      901 atatgagtat tctagcagaa gaattaaagt gaggaaagaa tgaaaatcaa taaaaaatat
      961 ctagcaggtt cagtggcagt ccttgcccta agtgtttgtt cctatgaact tggtcgtcac
     1021 caagctggtc aggttaagaa agagtctaat cgagtttctt atatagatgg tgatcaggct
     1081 ggtcaaaagg cagaaaattt gacaccagat gaagtcagta agagagaggg gatcaacgcc
     1141 gaacaaattg ttatcaagat tacggatcaa ggttatgtga cctctcatgg agaccattat
     1201 cattactata atggcaaggt tccttatgat gccatcatca gtgaagaact tctcatgaaa
     1261 gatccgaatt atcagttgaa ggattcagac attgtcaatg aaatcaaggg tggctatgtg
     1321 attaaggtag acggaaaata ctatgtttac cttaaagatg cggcccatgc ggacaatatt
     1381 cggacaaaag aagagattaa acgtcagaag caggaacgca gtcataatca taactcaaga
     1441 gcagataatg ctgttgctgc agccagagcc cagggacgct acaccacaga tgatggttat
     1501 atctttaatg cttctgatat cattgaggac acgggtgatg cttatatcgt tcctcacggc
     1561 gaccattacc attacattcc taagaatgag ttatcagcta gcgagttagc tgctgcagaa
     1621 gcctattgga atgggaagca gggatctcgt ccttcttcaa gttctagtta taatgcaaat
     1681 ccagttcaac caagattgtc agagaaccac aatctgactg tcactccaac ttatcatcaa
     1741 aatcaagggg aaaacatttc aagcctttta cgtgaattgt atgctaaacc cttatcagaa
     1801 cgccatgtgg aatctgatgg ccttattttc gacccagcgc aaatcacaag tcgaaccgcc
     1861 agaggtgtag ctgtccctca tggtaaccat taccacttta tcccttatga acaaatgtct
     1921 gaattggaaa aacgaattgc tcgtattatt ccccttcgtt atcgttcaaa ccattgggta
     1981 cgagattcaa gaccagaaca accaagtcca caatcgactc cagaacctag tccaagtccg
     2041 caacctgcac caaatcctca accagctcca agcaatccaa ttgatgagaa attggtcaaa
     2101 gaagctgttc gaaaagtagg cgatggttat gtctttgaga agaatggagt ttctcgttat
     2161 atcccagcca agaatctttc agcagaaaca gcagcaggca ttgatagcaa actggccaag
     2221 caggaaagtt tatctcataa gctaggagct aagaaaactg acctcccatc tagtgataga
     2281 gaattttaca ataaggctta tgacttacta gcaagaattc accaagattt acttgataat
     2341 aaaggtcgac aagttgattt tgagg
//
LOCUS       DAVKFH010000115         2014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKFH010000115 DAVKFH010000000
VERSION     DAVKFH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.115"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..91
                     /locus_tag="VW006_002069"
     CDS             <1..91
                     /locus_tag="VW006_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HEU9380849.1"
                     /translation="IEVSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            106..846
                     /locus_tag="VW006_002070"
     CDS             106..846
                     /locus_tag="VW006_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380850.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            849..>2014
                     /locus_tag="VW006_002071"
     CDS             849..>2014
                     /locus_tag="VW006_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380851.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMA"
BASE COUNT          660 a          260 c          368 g          726 t
ORIGIN      
        1 tattgaagtt tcacatcatt atgacttaaa tgacaagaga tacactgata tatataaatt
       61 ggaaaatgga acacttttca aaattaagtg attggaggca agaatatgga taaagattat
      121 atattaaaag tgaaagggct gtatcatcaa tttttactag gaaataataa aacgttgcaa
      181 gtgctgaaaa atgtttctct ttctgcttcg agaggagaat ttataagtat tctaggaatt
      241 agtggttctg gaaagtcaac tttattaaaa tgtatttcta gtttgcttga accaacaagt
      301 ggggaagtaa ttttaaatgg aatcaacccc tataaaatca gaaatgcaaa attgtcaagt
      361 ataagacgta acgaagtatc ttttatattt caagcataca atttaatacc ttccctgccg
      421 gtaatagaaa atatagcact tcctttgcga ttatcacaaa aaaaattaac tattaaaaat
      481 gtagaaaact tactcaaaag aatgaagttt aatgctggct taaacgattt tgttggaact
      541 ctgtctggtg gagaacaaca gaaggttgct atagctagag cggttattgc tgatagtgat
      601 ataatatttg ctgatgagcc aactggggct ttagacagcg tttctcgtga agtaattttt
      661 gaattattga gagagttagt aggggcgggt aagtgtgtaa ttatggtaac gcacgatata
      721 gaattggcct cgaaaactga tcgtgcatta atattgaaag atggaaaaat tttcaaagaa
      781 cttcatagac ctagcgggga agagttgtat aaaatcttag aggtacaatc aactacggag
      841 gaatagagat gctcagatta gtttactatc aatttttaca taataagaaa caatggttag
      901 gcgtttcacc agtaattttt gtttcgagtt tagttatggg attggctgtt aatggggtaa
      961 ttaatgttga gaataattcg caagtatttg tcggacttcc agatcctaaa ccaattttta
     1021 tgtttccgat agtattcgga ggagtaacat tattctttgt gctctctaac ataataaata
     1081 tgttggttga aatttttaga gatgattacg aacttctgga ggtgttggga gcaagtctcc
     1141 ttcaactatc atttcttgtg gggggacaga tttttatcat ttcctcgatt attagtttta
     1201 ttgcatatct atgctctata tttgttacgt caaattatta ttatttttta caatactttt
     1261 tcggggaaaa tatattacct gatatccaat ttcaaacgag tgctgttgga tgtatcatta
     1321 cagtagtgtt gatttcattt ttagcctttc taagtggttg cttttatacg ttcaagaaga
     1381 ttagaaatag gaagagttct aaaataaggc atgttctttc aatagtaaaa agaatactgc
     1441 tattggctgg ttttagtgtt atctggttgc tttctttgca acaaattttc caagatagca
     1501 caattttagc gaaggcccag ataatattca atattgtaat cttagatatt gtgattattt
     1561 atcaattatc tccttttata cagtcatgtt ttatcaaact actttctata attatattta
     1621 gaaataattt tatgttcatt gtttcgaagt ggaatttact atatcgtaag ccttatataa
     1681 aatctatcag tgctgcaatt acaggagcta tattactaat ttctagtttt caaatgattt
     1741 ctcagaatat actatctcaa tttcaagatg acagtgattt agagctaaaa gtggcattta
     1801 ttgtctatgt aggagcacca attttgatag tacttgcaaa tattatttca attgcatttt
     1861 tgtcatcgca tcaagaaagg attgagattc agcaatttga aattttagga acttctaatt
     1921 accagatggt taagattaaa gttggtgagg ctatattttt gacatttgtt acttcattaa
     1981 ttgcattttt acttaacatt aaaattatgg cttt
//
LOCUS       DAVKFH010000116         1981 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKFH010000116 DAVKFH010000000
VERSION     DAVKFH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.116"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            24..>1981
                     /locus_tag="VW006_002072"
     CDS             24..>1981
                     /locus_tag="VW006_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9380852.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETK
                     PAVTTTERSAVDSAILPISTAAKVETTSAPASTNEILKPSLSLSDARQNPAIRKEDVD
                     RGYSGFRTAGSGFRAAGSGFRAAGPENKPILNPNNTIAFSDISQGLHSFRGIGHSRGG
                     REIHYDVTTVRRGNRLNFTIKYSGPGEFVNNNFILDKGDGFGNPSNATITSSNPRVRE
                     QSKSISQGANYVSHSGYSMTSATSTNTEQTIRFSLPIINPNGDLSVRLKPVTFNVDQG
                     GGGAATSNDPYSNSNYYHRANPLLLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQ
                     TRLVREGEKGQRQITYKVHRFGNETLLGLPISNRVTKEAKPRIMQIGVAKELIDTVKP
                     RVDQNKVGDTNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAK
                     GEKGDRGERGLTGAQGAKGEKGDRGERGL"
BASE COUNT          692 a          354 c          425 g          510 t
ORIGIN      
        1 aaaatactat ctaggagttt aaaatgaaac attcacataa aaaatcattt gactggtata
       61 gcatgcaaca acgttattct attcgtaagt attactttgg tgcagttagt gtcttgctcg
      121 gtaccgcttt ggtattaggt gcagcagcta gtgtccaaac agtacaagcg gaagaaaaca
      181 aacaagaaac taccaatagt atttctgttg gtaggggaga agcagctact aaaccagcag
      241 aggtttctgc gtctaataaa gagaaaacct atgcagctcc aactgtagct aatccagtag
      301 aaacgactcc agttaaaact ggagaagtta ctaaaccagc agaaaaagtt gaagaagcaa
      361 aagacaaaaa agaggaagta acgcatcaag atgctattga caagtcaaaa ttgttaacgg
      421 ctctttcgcg tgctaaaaag ttagaaagca agttatatac agaagcaagt gctgctaact
      481 tgcaaacaag tatccaagct ggtcaaagct tgcttgggaa agcagatgca tctgaagctg
      541 aattatcagc agcagagtca tctattcaat catctattat tggtctagaa cttcgttcta
      601 actctaacaa aggaactgta tcagaaacgc ctgtagcaaa gaaagctaat atagttgaag
      661 cgaaggaaga gactaaacca gctgtaacaa cgactgaacg ttcagctgtt gatagcgcta
      721 ttttgccaat tagcacagct gctaaagtag aaacaacttc agctccagca tctactaatg
      781 aaatcttgaa accaagtttg agcctttctg atgctcgcca aaatccagct atccgtaagg
      841 aagatgttga tagagggtat agtggtttta gaacagcagg aagtggcttt agagcggctg
      901 gaagtggttt tagagcagct ggaccagaaa ataaaccaat cttaaatcca aataatacta
      961 ttgcattttc tgacatcagt cagggcttac attcatttcg tggtatagga cattcacgtg
     1021 gtggtcgtga aatccattat gatgtaacga cagtacgtag aggtaataga ttgaatttca
     1081 caattaaata ctctggtcca ggtgaatttg ttaataataa ttttatcttg gataaagggg
     1141 atggatttgg aaatccttca aatgcaacga tcacaagttc aaatccaaga gtaagggagc
     1201 aatcaaaatc tattagtcag ggtgccaact acgtatctca ctcaggatac agtatgacct
     1261 ctgctacttc aacaaatacg gaacaaacta tcagatttag tttgcctatc attaatccaa
     1321 atggtgattt gtctgttcgt ttgaaaccag ttacttttaa cgtggaccaa ggtggtggtg
     1381 gtgccgctac tagcaatgac ccatacagta actccaacta ttatcataga gcaaatccac
     1441 tcctcttgga tgcaaaccct tatggtggta ctaataataa gactgtttca gaagacattg
     1501 acttccaaac tgtctatctt ccaactagta agttaccaga aggtcaaact agattagttc
     1561 gagaaggtga aaaaggacaa cgtcaaatta cctataaagt acatcgattt ggtaacgaaa
     1621 cacttttagg attgccaatt agtaatagag ttactaaaga agctaagcca cgtattatgc
     1681 aaattggtgt ggctaaagag ttaatcgata cagtaaaacc acgtgttgat caaaataaag
     1741 ttggtgatac aaataacctc actttctatc ttgataacga tggaaacggt gtttatactg
     1801 agggcgtaga cgaacttgtt caaaaaattg ctattaaaga tggagccaag ggtgaaaaag
     1861 gagaccaagg tgaacgcggt ctaactggag ctaaaggtga aaaaggagac cgaggcgaac
     1921 gcggtctaac tggagctcaa ggagctaaag gtgaaaaagg agaccgaggt gaacgcggtc
     1981 t
//
LOCUS       DAVKFH010000117         1889 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKFH010000117 DAVKFH010000000
VERSION     DAVKFH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.117"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(151..1781)
                     /locus_tag="VW006_002073"
                     /pseudo
     CDS             complement(151..1781)
                     /locus_tag="VW006_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
BASE COUNT          577 a          367 c          397 g          548 t
ORIGIN      
        1 gatttctaac aatgttttag aaatagaggt gtactattct agtttcaata tactatatat
       61 ctgcagaata ctctttcagt ttcattcagc ctagtcttgc ttaggaaaaa gtaagaagcc
      121 atccaattat gatggatagc ttcttggtta ttagatttgt tctgcaaaaa ccttttcagc
      181 aaggttcata gcgtggtctg atacacgagt gtagtgtgag atgatgtcga taaagttgac
      241 cccagcttgt gttgaacatt cgcctttgtt gaggcgtttg atgtgggttt ttctgagaac
      301 acgttctatc ttattgattg cttcatgacg ttctacaaga ctgcgtgctt tttcaatatc
      361 attgttttcc acactatcca gagcatcttt gataaagtca ctagtttggc ggtaaacttc
      421 ctctaattct ttcaaggcgg catcagaaaa ttcaacattt ttccgttgaa gatagtcagt
      481 cagattgagt agagcctccg tgtggtctcc aatccgttcc aaatcacggg aggaatcaag
      541 gatattggtg agcacttcac tttctttttg gctgagagct tcgcttgaaa gggcaatgag
      601 ataacgtgtt aattgctcat cgatggtgtt aattgcttct tcggttttat gccctttttc
      661 agcaactttt tcatccaagt caatgatgta cttataggaa aggtcaaagg ctttagcagc
      721 gtagtttcct aagtgcaaga gctctttttt agcatttcct agagcgatag atggggcttg
      781 tttgatgaaa tgttcatcaa gatataaggg ttcgtatttg acaacctcgt cctctccagg
      841 aataatcttg gttacaaagt aagccagagc tccgataaat ggaaattgga caatggtgtt
      901 ggtaatatta aggttccgtg agcaaaggcg atggtcattt ccggtgctag atttagcgta
      961 gcttcaaacc aatgaatcag gacagtaaaa ggaactagaa aaataacgca gacgactgtt
     1021 ccgataacgt tgaaggcaac atgagctcct gctacccgtt tagctgcaat attagcccct
     1081 aaagaggcaa tgatggctgt aatggttgtc ccgatattgt caccaaatag aactggcaaa
     1141 gctccctgta ggtcaattag attgccggcg tagaggtttt gtaaaatccc aatggtagcc
     1201 gaagaagctt gaattagcaa ggtcaagccg gtaccgacaa agacacccaa aacaggattc
     1261 ttacttagct caatcatata gtccttaaag acctgtaaat ccttgagtgg agccattgcg
     1321 ccgctcatga gattgagggc aaaaaagata ccaccgacac caaagaggat gcgtccgata
     1381 ttattgactg tccgattttt cgtaaaaaag agacagacgg caccgataaa gagcataggt
     1441 agggcatagt tacctaattt aaaaccgatg agaaaggatg tgacagttgt cccaatatta
     1501 gcacccatga caatcccgat agcctgacgt aaggttaaga gaccggcact gaccaggccg
     1561 actgtaataa ctgttacacc agaactagac tgaattagag cagtcatccc aataccaacc
     1621 agaactccaa agaaaggatt actagtatat ttgtcaatgt aaaaacgaag gcgatctcca
     1681 gcagcttgtt gcaaaccgtc tcccatggtc ttgatgctat ataagaatag ccccagacca
     1741 cctaaaaagt gaaataaaat ttcctgccaa ttaatggaca tttctttttc ctccgaaaaa
     1801 taatcgcgga atatctccta ttctatttta aaggataaaa gtaaatctaa caagtgttaa
     1861 tgtcaagttt gtaaaagaaa attataatt
//
LOCUS       DAVKFH010000118         1799 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKFH010000118 DAVKFH010000000
VERSION     DAVKFH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.118"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..991)
                     /locus_tag="VW006_002074"
     CDS             complement(<1..991)
                     /locus_tag="VW006_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380853.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEA"
     gene            complement(1001..>1799)
                     /locus_tag="VW006_002075"
     CDS             complement(1001..>1799)
                     /locus_tag="VW006_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9380854.1"
                     /translation="RNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISF
                     LDDDDEYMSDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQ
                     MVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYA
                     HSKENGITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDK
                     CKALKVIKKAREEKIFNEFLFLKYMLLYYFGFFYCIYDNYVQLKFRK"
BASE COUNT          623 a          318 c          211 g          647 t
ORIGIN      
        1 ttgcttcata taacggaaaa tctctattca cctcatttgg ttcgatatca ttcattttat
       61 ttgactcaat tggattaatt gaatacaatt ttaaaaaaat aggcttcgta ccaataatag
      121 aggataattg acctcctaat tcacctagcg ctgtaaaaat aacacctgtt agtgctccta
      181 ttgcttcaat agtaccaatt ttcactattc cttttattgc aagatagcct gttaaaaaaa
      241 cgagagatat ctgaaaaaaa atattgagaa agaagctaat agcgcctgct aacgtttcta
      301 cagttgtctt tctttgtata accatcttta ataaaattcc tgcttcttta attttcttag
      361 gcaatacata taaaagattc aaggatgcta acacatcaaa tccattcaat atagtctcac
      421 tagattttaa aaaagcttca ttttggttag ttaaatttag actaacttct cgcattttcg
      481 atgcaaagat ttttggtaca agtagcataa tcattaatga aaacaaggtg gctacagtca
      541 atgaccaatg atagtgatta agagtcacaa ctgcaaatat agtaccagaa attcctttta
      601 ttactaaaaa aagttgttta aaagcctgat catttaaagt ctgaacatca ttatttagcc
      661 acgaaagata tgttcctgat gatttactat gaaattcttg ataggtagag ttagagatgt
      721 ctgtggcaac tctatttcga atctctagat taaactcttg gatcacttca acctgataat
      781 ttttcactac ccagtcaagg aatattatcc cacaccagac aatcatttgg tagattgaca
      841 atttcaaaaa ccgctctaaa ttcatcgcaa ttaattcatt caacaccaga gcattaatag
      901 ttgctgcata aattagcaat aattgaccag caacaataaa tatcgttaat aaactaaatt
      961 tttttatatt tgattttata atagtataca caatagtttc tcactttcta aattttaatt
     1021 gaacatagtt gtcatatata caatagaaaa aaccaaaata atataataac atatatttca
     1081 aaaaaagaaa ttcgttaaaa attttttctt ctcttgcctt cttgattact tttaaagcct
     1141 tgcatttgtc tcctattaat agtaaccgct ttatgtttaa agaataatat ttctttgtaa
     1201 ccaatattct ctcgttgaaa ctcaataaat taaaatattt cctacagtaa ttataatatt
     1261 cttcatctgc attaattgtt ttttgtgtca ctccagtgat accgttttct ttactgtgag
     1321 cgtagtaatt caccaagaat tctcgcacta tatcaatttg gtatccttga gcaagtagtt
     1381 ttaataaaac aacaccgtcc tgatgtgaat ctattttctc aaaaccatta attaattcta
     1441 gcacctcttt tttacacaac caaaatgacg tacctgctat attgtgaacc atttgaacaa
     1501 acaagggatt tccaacaaaa tcggtcttct cctcttctcg tgtaccattt ggataaatta
     1561 ttattccata actataaact aaagctaaat tcttcattct actcttttta aaacaagcca
     1621 tcaactttaa aattcgatct gacatatatt catcatcatc gtctaaaaat gatatatact
     1681 tacctctaga atttttgata cctatgtttc tggcattagt tgcacctaaa tcttcattac
     1741 ttaaaattaa cttaattcta tgattggtat agccaaattg atggataatt ttatttctt
//
LOCUS       DAVKFH010000119         1724 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKFH010000119 DAVKFH010000000
VERSION     DAVKFH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.119"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            338..1689
                     /locus_tag="VW006_002076"
     CDS             join(338..779,779..1689)
                     /locus_tag="VW006_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9380855.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
BASE COUNT          586 a          268 c          386 g          484 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagggatt ctctgaagtt atggatgtcg ttccaatatg tgtacgttgg aatgttagtg
      121 cttatatggg gggaacagaa tcctctcttg attgaagaca agttagccat taggctggtt
      181 tgtctctttg tcaactgtag tgggttgata taatagtatt agtgagtggg ataaaagttt
      241 catttagttt attcagtaca aatttaacgg gtcaagattt attgaactgc accccaatag
      301 ttagacagaa aaaatctaac ttttggggtg ttttattatg aaattgagtt atgaagataa
      361 agttcagatc tatgaactaa gaaagcaagg acaaagcttc aaacagcttt caaaaagatt
      421 tggtgtggat gtttctggtc taaagtacat ggtgaaatta attgaccgtt atggcataga
      481 aatcgttaaa aaagggaaga atcgtcacta ttcttctaaa ttgaaacaag aaatgatgga
      541 taaagcctta ctggaaggtt gttcacaaag aagtattagt cttgattatg ccctcccaaa
      601 ccaaggaatg ctttcatttt ggccggcaca atacaagaaa aacgggtata ctattgttga
      661 gaaaacaaga gggagaccag ctaaaatggg acgtaaacgg aagaagactt gggaagaaat
      721 gacagaacta gaacgactcc aggaggagaa tgaacgctta cggactgagg tggcctacct
      781 aaaaaagtta aaagagttag aggaaaggga cgaagcccta gagcgagaaa ggcagagaca
      841 gttagagaaa tggtttcagg aggatttcga ctagattttc ttcttgaaac agcacgttta
      901 gctcgctcga cttactatta tcagttgaaa caactggatg gggttgataa agataaagag
      961 attaaaaccg aaattcaggg cattgataat gaacataaag gcaactatgg ctatcgtcga
     1021 atccatttag aactaaggaa tcgtggtttt gtggtcaatc ataagaaagt tcaacggttg
     1081 atgagaattc ttggtttaac ggctcgaatt cgccgtaaac ggaagtattc ttcctaccaa
     1141 ggagagattg gcaagaaagc agagaatctc attcaacgcc agttcgaagc atcgaggcca
     1201 atggaaaagt gctatacaga tgtgacagag tttgccattc caaatagcac acagaaactc
     1261 tatttatcac cagttttaga tggttttaac agtgaaatca ttgcttatca tctttctact
     1321 tcgcccaact tagaacaagt gaagagtatg ttagaacaag cctttacaga gaaatactat
     1381 gagaatacga ttctccatag tgaccaaggc tggcaatacc aacacgattc ttatcatcgg
     1441 ttcctagaga gtaagggaat tcaagcatcc atgtcacgca agggcaacag cccagacaac
     1501 ggcatgatgg aatctttctt tggcattttg aaatcggaga tgttttatgg ttatgagaag
     1561 aactttagat ctttagaaaa ccttgaacaa gctattgtgg actacattga ttactacaac
     1621 aacaaacgaa ttaaggtaaa actaaaagga cttagttctg tgcagtacag aactaaatcc
     1681 tttggataaa ttatttgtct aattttttgg ggtcagtaca gtat
//
LOCUS       DAVKFH010000120         1661 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKFH010000120 DAVKFH010000000
VERSION     DAVKFH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.120"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            145..1491
                     /locus_tag="VW006_002077"
     CDS             145..1491
                     /locus_tag="VW006_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9380856.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          491 a          359 c          325 g          486 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gctgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tattggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa
      541 ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc
      601 acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat
      661 catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct
      721 ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt agaacggttt
      781 aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt
      841 gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt
      901 ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc
      961 tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc
     1021 tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg
     1081 agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc
     1141 atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa
     1201 aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat
     1261 ctagctggag gtgacttcca aactttaaca atcaaacgct tccgccatct ttttcttcac
     1321 gtggtaggaa aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc tagtctctat
     1381 gcctattccg aattgttttc agcactttat tctaggatta gaaaagtcaa cctgaatctt
     1441 cctgttcctt atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta aagaacagtc
     1501 gagatgaaaa aatcgtgtga cgaaccaagg gaggagtctg cccttttgag gaaatctagc
     1561 gaggaaaaac gatactggaa cagcagaaag taaaactgac ctcatgagga ggaagaaagt
     1621 ggctcatgag gtcaggggtt ttgtaagtta catctagttg a
//
LOCUS       DAVKFH010000121         1618 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKFH010000121 DAVKFH010000000
VERSION     DAVKFH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.121"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            84..491
                     /locus_tag="VW006_002078"
     CDS             84..491
                     /locus_tag="VW006_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9380857.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VW006_002079"
     CDS             656..955
                     /locus_tag="VW006_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9380858.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VW006_002080"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VW006_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9380859.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          554 a          323 c          283 g          458 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatca atttgtcc
//
LOCUS       DAVKFH010000122         1555 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKFH010000122 DAVKFH010000000
VERSION     DAVKFH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.122"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(129..216)
                     /locus_tag="VW006_002081"
     tRNA            complement(129..216)
                     /locus_tag="VW006_002081"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Ser,seq:gct)
     gene            complement(223..296)
                     /locus_tag="VW006_002082"
     tRNA            complement(223..296)
                     /locus_tag="VW006_002082"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Ile,seq:gat)
     gene            complement(332..402)
                     /locus_tag="VW006_002083"
     tRNA            complement(332..402)
                     /locus_tag="VW006_002083"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Gly,seq:tcc)
     gene            complement(422..494)
                     /locus_tag="VW006_002084"
     tRNA            complement(422..494)
                     /locus_tag="VW006_002084"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(459..461),aa:Phe,seq:gaa)
     gene            complement(498..571)
                     /locus_tag="VW006_002085"
     tRNA            complement(498..571)
                     /locus_tag="VW006_002085"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Met,seq:cat)
     gene            complement(582..671)
                     /locus_tag="VW006_002086"
     tRNA            complement(582..671)
                     /locus_tag="VW006_002086"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(633..635),aa:Ser,seq:tga)
     gene            complement(682..755)
                     /locus_tag="VW006_002087"
     tRNA            complement(682..755)
                     /locus_tag="VW006_002087"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Ile,seq:cat)
     gene            complement(769..842)
                     /locus_tag="VW006_002088"
     tRNA            complement(769..842)
                     /locus_tag="VW006_002088"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Met,seq:cat)
     gene            complement(848..921)
                     /locus_tag="VW006_002089"
     tRNA            complement(848..921)
                     /locus_tag="VW006_002089"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(885..887),aa:Pro,seq:tgg)
     gene            complement(937..1010)
                     /locus_tag="VW006_002090"
     tRNA            complement(937..1010)
                     /locus_tag="VW006_002090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(974..976),aa:Arg,seq:acg)
     gene            complement(1020..1105)
                     /locus_tag="VW006_002091"
     tRNA            complement(1020..1105)
                     /locus_tag="VW006_002091"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1069..1071),aa:Leu,seq:taa)
     gene            complement(1113..1184)
                     /locus_tag="VW006_002092"
     tRNA            complement(1113..1184)
                     /locus_tag="VW006_002092"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1150..1152),aa:Gly,seq:gcc)
     gene            complement(1207..1279)
                     /locus_tag="VW006_002093"
     tRNA            complement(1207..1279)
                     /locus_tag="VW006_002093"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1244..1246),aa:Thr,seq:tgt)
     gene            complement(1298..1379)
                     /locus_tag="VW006_002094"
     tRNA            complement(1298..1379)
                     /locus_tag="VW006_002094"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1343..1345),aa:Leu,seq:tag)
     gene            complement(1385..1457)
                     /locus_tag="VW006_002095"
     tRNA            complement(1385..1457)
                     /locus_tag="VW006_002095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Lys,seq:ttt)
     gene            complement(1482..1554)
                     /locus_tag="VW006_002096"
     tRNA            complement(1482..1554)
                     /locus_tag="VW006_002096"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1519..1521),aa:Asp,seq:gtc)
BASE COUNT          427 a          434 c          339 g          355 t
ORIGIN      
        1 gaaatttgtc ctttctcgag cttagctttt cttcaaccca ctacagttga caaagagcct
       61 taagtttacg gatctgaagt tttattgtct ctaaaaacaa aaaagagagt tacaaactct
      121 cattaaaacg gagaataagg gattcgaacc cttgcgccag ttacccgacc taacgattta
      181 gcaaaccgtc ctcttcagcc tcttgagtaa ttctccaatt aatgggcacg agtggactcg
      241 aaccaccgac ctcacgctta tcaggcgtgc gctctaacca cctgagctac gcgcccaagt
      301 taaaaaactt ggtaatttga acaaagttca aagcgggtga cgagaatcga actcgcgaca
      361 acagcttgga aggctgtagt tttaccacta aactacaccc gcataaatac tatcaataaa
      421 atggcgcgag acggaatcga accgccgaca catggagctt caatccattg ctctaccaac
      481 tgagctaccg agccttattg cgggagcagg atttgaacct acgaccttcg ggttatgagc
      541 ccgacgagct accgagctgc tccatcccgc gttaatataa aaggaggatg tgggattcga
      601 acccacgcac gcttttacac gcctgacggt tttcaagacc gttcccttca gccggacttg
      661 ggtaatcctc caatattcaa atggaccttg taggacttga acctacgacc actcggttat
      721 gagccgagag ctctaaccag ctgagctaaa ggtccgacaa gatcattata gcggcgaagg
      781 ggatcgaacc cccgacctcc cgggtatgaa ccggacgctc tagccagctg agctacaccg
      841 ccatgaatcg ggaagacagg attcgaacct gcgacacctt ggtcccaaac caagtactct
      901 accaagctga gctacttccc gagttaaata gaaaaatgca ccctagagga gtcgaacctc
      961 taaccgcctg attcgtagtc aggtactcta tccagttgag ctaagggtgc tccatattat
     1021 gccgaggacc ggaatcgaac cggtacgatc gttaccaatc gcaggatttt aagtcctgtg
     1081 cgtctgccag ttccgccacc ccggcctctc taagcgaacg acgggattcg aacccgcgac
     1141 ccccaccttg gcaaggtggt gttctaccac tgaactacgt tcgcactgtt ttcttctatc
     1201 taaaaatgcc ggctacatga cttgaacacg cgaccctctg attacaaatc agatgctcta
     1261 ccaactgagc taagccggct catttgttat atcttaatgc gggttaaggg acttgaaccc
     1321 ccacgccgtt aagcgccaga tcctaaatct ggtgcgtctg ccaattccgc caaacccgca
     1381 tatatgaccc gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc
     1441 aactgagcta acgagtctaa aataacttgc gttatcttaa acggtcccga cgggaatcga
     1501 acccgcgatc ttcgccgtga cagggcgacg tgataaccgc tacactacgg gacct
//
LOCUS       DAVKFH010000123         1532 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKFH010000123 DAVKFH010000000
VERSION     DAVKFH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.123"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            24..1370
                     /gene="gdhA"
                     /locus_tag="VW006_002097"
     CDS             24..1370
                     /gene="gdhA"
                     /locus_tag="VW006_002097"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9380860.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          472 a          297 c          323 g          440 t
ORIGIN      
        1 tatgacaata aaaggagaac gatatgacat ctgctaaaga atatatccaa agcgtgtttg
       61 aaactgtgaa agctcgtaac gggcacgagg ctgaattcct ccaagctgtt gaagaatttt
      121 tcaacacttt agaacctgta tttgaaaaac accctgagta tatcgaagaa aatatcttgg
      181 cacgtattac tgagcctgag cgcgtggttt ctttccgtgt tccttgggtt gaccgtgatg
      241 gaaaaattca agtaaaccgt ggttaccgtg ttcaattcaa ctcagctgtt ggaccataca
      301 aaggtggact tcgtttccac ccaactgtaa accaagggat cttgaaattc ctcggatttg
      361 aacaaatctt taaaaatgtc ttgactggac ttcctatcgg tggaggtaaa ggtggatcag
      421 acttcgatcc taaaggtaaa acagatgctg aagtgatgcg cttctgccaa agcttcatga
      481 ctgaattgca aaaacacatc ggaccatcac ttgacgtacc tgctggtgat atcggtgttg
      541 gtggacgtga aattggttac ctttacggtc aatacaaacg tcttaaccaa tttgatgctg
      601 gtgtcttgac tggtaaacct cttggatttg gtggtagctt gattcgtcca gaagcaactg
      661 gttacggttt ggtttactat actgaagaaa tgctcaaagc taacggtaac agctttgctg
      721 gtaagaaagt cgttatttca ggttctggta acgttgctca atatgctctt caaaaagcaa
      781 ctgaactcgg tgcaactgtt atttctgttt ctgactcaaa tggttatgtc atcgatgaaa
      841 atggtatcga cttcgatctt ttggttgatg ttaaagaaaa acgtcgtgct cgtttgactg
      901 agtatgcagc tgaaaaagca actgcaacct accacgaagg tactgtatgg acttacgctg
      961 gaaactatga cattgctctt ccatgtgcca ctcaaaacga aatcaacggt gaagcagcta
     1021 aacgtttggt tgctcaaggc gttatctgtg tatctgaagg tgccaacatg ccgagcgacc
     1081 ttgatgccat caaagtttac aaagaaaatg gtatcttcta cggacctgca aaagctgcca
     1141 acgctggtgg tgtagccgtt tcagctcttg aaatgagcca aaacagtctt cgcctctcat
     1201 ggactcgtga agaagttgat ggccgtctca aagacatcat gacaaacatc ttcaacacag
     1261 ctaaaacgac ttcagaaaca tacggtcttg ataaagacta ccttgcagga gctaacattg
     1321 ctgcctttga aaatgtagca aatgccatga ttgcacaagg tattgtttaa gatttattta
     1381 ctcaatcctc aatcactacg attggggatt tttagcttga attaaaaaat tggaaaatct
     1441 aattaaataa aatgaaatga aataaaaact gaacaaattg attgcggaat tcaaaccaat
     1501 ttctaacata tttctagaaa ttaatttgac tt
//
LOCUS       DAVKFH010000124         1361 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKFH010000124 DAVKFH010000000
VERSION     DAVKFH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.124"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>1361)
                     /gene="lanM"
                     /locus_tag="VW006_002098"
     CDS             complement(<1..>1361)
                     /gene="lanM"
                     /locus_tag="VW006_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEU9380861.1"
                     /translation="DKNGNIFSSLVFYLEDLIYPWIVKPLVLEINSLREKGLLEGESE
                     QQRYKYFITLFDKEENILNFYNKYPVLLRQISESCLRFYTYFIEILSNLENDFSVLEE
                     ELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKIVYKPKNLIINNSLNTIAEYIRKV
                     DEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKKLPEYYYNFGVLLAFIYLFNGSDIHF
                     ENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTRTLLLPTEGVKREDGLD
                     VEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKNLPVRDGDIEFDKYIKD
                     FKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFRDTNSYASVLSFLYHPDFYE
                     EMLDREKALENLWSNKFSNQGIVASECEQMRLLDIPIFYTDTNINEIYDDFGNHFISL
                     HQF"
BASE COUNT          469 a          253 c          155 g          484 t
ORIGIN      
        1 aaaattgatg tagggaaata aaatgatttc caaaatcatc gtagatttca ttgatatttg
       61 tatctgtata aaaaatcgga atatctaata acctcatttg ttcacattcg cttgctacaa
      121 taccttgatt agaaaactta ttgctccaga gattttccaa tgctttttct ctatctaaca
      181 tttcttcata aaaatcagga tgatataaaa aagatagtac tgaagcatag ctatttgtgt
      241 ctctaaaaag tacccttgtc tttaaaccat acaagtttgc ttttaatagc attttaaatt
      301 cttctgtttt atttaactct tcaaatatca gataaaaatc cctaaaacct tttttgaaat
      361 cttttatata cttatcaaat tctatatcac catcccgaac aggcaggttt ttcccacctt
      421 caaaatcaat tttcccaata tcaaacttta ccttatcagt atttaaatta attaaaactt
      481 gaccattaaa agcatccttc ttaaaatttc ctgtaagagc actcatctca acatcaagac
      541 catcctccct ttttactccc tctgttggaa gaagcaaagt tcgagtcacc ctgtgaaaca
      601 gggtatccag caaactctga ccagttttat catcaaatag aggttgctgt aacattgttt
      661 caaagtctat tattacaggc atatctccat aggaaattaa attttcaaaa tgtatatcac
      721 tcccattaaa taaatatata aatgctaaaa gcacaccaaa attataataa tattcaggta
      781 attttttctt ttgctctaga ggtagataat caataaattc ttcatagctg tgatccgaat
      841 aagcaatagt tcgaggtatt cttatcctaa ttttttcatc aacctttcgg atatactcag
      901 caatagtatt tagtgagtta ttgattatta aatttttagg cttgtaaaca attttcgcgt
      961 catcaaagaa gagtatcaaa acagtttttc cttggctgtg tgtatcaccc tttccaaatt
     1021 ttatatcatt taatttcccc cttagcccta attcttcttc tagcacacta aaatcatttt
     1081 ctaaatttga taaaatttct ataaaataag tatagaaccg aagacaagac tccgatattt
     1141 gcctcagtaa aacgggatat ttgttataaa aatttaatat attctcttcc ttgtcaaaca
     1201 atgttataaa atatttgtac cgttgctgct ccgattcccc ttcaagtaaa cctttttcac
     1261 gcaatgaatt tatctctaaa accaaaggtt taacaatcca aggatagatt aaatcttcta
     1321 aataaaatac tagagaggaa aaaatatttc cattcttgtc a
//
LOCUS       DAVKFH010000125         1353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKFH010000125 DAVKFH010000000
VERSION     DAVKFH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.125"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..459
                     /locus_tag="VW006_002099"
                     /pseudo
     CDS             1..459
                     /locus_tag="VW006_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            587..>1353
                     /locus_tag="VW006_002100"
     CDS             587..>1353
                     /locus_tag="VW006_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEU9380862.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQF"
BASE COUNT          446 a          280 c          218 g          409 t
ORIGIN      
        1 catgaataca aggctatcaa gcgctactgg aaactcattc aacaggatag tcgtaaactc
       61 agcgataaac atttttatcg ccctactttt cgtatgcatt taaccaataa agagatttta
      121 gacaagcttt tgagctattc acaagacttg aaacaccact atcagctcta tcaactcttg
      181 ctgtttcact ttcagaataa ggaaccagag aaatttttcg gacttattga ggacaatctt
      241 aagcaggttc atcctatttt tcagactgtc tttaaaacct tcctcaaaga taaagaaaag
      301 attgtcaacg cccttcaact acactattct aatgccaaac tggaagcgac caataatctc
      361 atcaaactta tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt caaaaaacgg
      421 attttcatcg ctctgaacat caaaaaagaa aggacgaaat ttggctctat aatatttgta
      481 gtgggtaaat cccctatgga tattatggag cttatttttg tgtagaaaaa aagtcccata
      541 tgacctataa tgaaaagcga caaaacaact cattagaaag aatcatatgg aacaattaca
      601 ttttatcaca aaactgctcg atattaaaga cccaaacatc aagattctag atatcatcaa
      661 tatggatacc cacaaagaaa ttatcgctaa gctggattat gaggctccat cttgccctga
      721 ttgtggaagt ctaatgaaga aatatgactt tcaaaaaccg tctaagatcc cttacctcga
      781 aacaactggt atgccttcta gaattctcct tagaaaacgc cgtttcaagt gctatcactg
      841 ttcaaaaatg atggttgctg agactcccct ggtaaagaaa aatcaccaaa tccctcgtat
      901 catcaaccaa aaaattgctc aaaagttaat tgaaaaaatt tctatgactg atattgccca
      961 tcagctggcc atttcaactt caactgtcat tcgcaagctc aatgactttc actttgagca
     1021 tgatttttct cggcttccaa agattatgtc ttgggatgag tatgccttca ctaagggaaa
     1081 gatgagtttc attgcgcaag attttgataa tcttaatatt atcactgttc ttgaaggcag
     1141 aacacaagct gtcatccgaa atcactttct tcgctacgat agagccgttc gttgtcaagt
     1201 gaaaatcatt acgatggata tgtttagtcc ttactatgac ttggctaaac agctttttcc
     1261 gtgtgctaaa atcgttctag atcgtttcca tattatccaa catctcagcc gtgccatgag
     1321 tcgttttcgt gttcaaatta tgaatcagtt tga
//
LOCUS       DAVKFH010000126         1277 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKFH010000126 DAVKFH010000000
VERSION     DAVKFH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.126"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            53..>1277
                     /gene="pcpA"
                     /locus_tag="VW006_002101"
     CDS             53..>1277
                     /gene="pcpA"
                     /locus_tag="VW006_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9380863.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTF"
BASE COUNT          497 a          177 c          204 g          399 t
ORIGIN      
        1 gcaaagttat aattttatat ctattagaaa ataaatatgt aaggaaattt aaatgaaaaa
       61 aactacaata ttatcattaa ctacagctgc agttatttta gcagcatatg tccctaatga
      121 accaatccta gcagatactc ctagttcgga agtaatcaaa gagactaaag ttggaagtat
      181 tattcaacaa aataatatca aatataaggt tctaactgta gaaggtaaca taggcactgt
      241 tcaagtgggt aatggagtta ctcctgtaga gtttgaagct ggtcaagatg gaaaaccatt
      301 cacgattcct acaaaaatca cagtaggtga taaagtattt accgttactg aagtagctag
      361 tcaagctttt agttattatc cagatgaaac aggtagaatt gtctactatc ctagctctat
      421 tactatccca tcaagcataa aaaaaataca aaaaaaaggc ttccatggaa gtaaagctaa
      481 aactattatt tttgacaaag gtagtcagct ggagaaaatt gaagatagag cttttgattt
      541 ttctgaatta gaagagattg aattgcctgc atctctagaa tatattggaa caagtgcatt
      601 ttcttttagt caaaaattga aaaagctaac cttttcctca agttcaaaat tagaattaat
      661 atcacatgag gcttttgcta atttatcaaa tttagagaaa ctaacattac caaaatcggt
      721 taaaacatta ggaagtaatc tatttagact cactactagc ttaaaacatg ttgatgttga
      781 agaaggaaat gaatcgtttg cctcagttga tggtgttttg ttttcaaaag ataaaaccca
      841 attaatttat tatccaagtc aaaaaaatga cgaaagttat aaaacgccta aggagacaaa
      901 agaacttgca tcatattcgt ttaataaaaa ttcttacttg aaaaaactcg aattgaatga
      961 aggtttagaa aaaattggta cttttgcatt tgcagatgcg attaaacttg aagaaattag
     1021 cttaccaaat agtttagaaa ctattgaacg tttagccttt tacggtaatt tagaattaaa
     1081 agaacttata ttaccagata atgttaaaaa ttttggtaaa cacgttatga acggtttacc
     1141 aaaattaaaa agtttaacaa ttggtaataa tatcaactca ttgccgtcct tcttcctaag
     1201 tggcgtctta gattcattaa aggaaattca tattaagaat aaaagtacag agttttctgt
     1261 gaaaaaagat acatttg
//
LOCUS       DAVKFH010000127         1152 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKFH010000127 DAVKFH010000000
VERSION     DAVKFH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.127"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..805)
                     /locus_tag="VW006_002102"
     CDS             complement(<1..805)
                     /locus_tag="VW006_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEU9380864.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKIEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGENK"
     gene            885..947
                     /locus_tag="VW006_002103"
     CDS             885..947
                     /locus_tag="VW006_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEU9380865.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            1028..>1152
                     /locus_tag="VW006_002104"
     CDS             1028..>1152
                     /locus_tag="VW006_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380866.1"
                     /translation="MSLSYYYEINPSTDILKCIEELLYKEDKCFNNILKNWKDIR"
BASE COUNT          412 a          148 c          182 g          410 t
ORIGIN      
        1 acttattttc tcctaaaata tcgaagactt gtatggcttg tttcattttt tcaattccac
       61 tggttgattg gcaacgttta aattcaaagt aaccagttgc atagagaaaa actgtttgtt
      121 cataaaagtt tagttcatta tccaacaatg tctttatctt actaattaaa taggagcaat
      181 tagaaaattc ttccttatca atgcttagaa tgaagcaatt aatagcaagc tgagagacaa
      241 tctgcttatt agttttattc agagatgagt aaatatagtt atttaacatc tctttagtta
      301 ataagaagta ggagttgtag ttaattgtgc gtacacagtt acctaataaa atgatttcat
      361 aatagcccca tttttcaatt ttgaaaagat agtctgtcag atgaagaagc tcttcatctg
      421 atggaatgat gttcgaatcc attgtgtgta aaatagattt taccattgtt ttctcaaaag
      481 aatttagagt gtaatcagaa gtatcagaaa gtagacatgc tatattattg atactttgtg
      541 atgaatattg cttacgaatg tactgtacta gattagcaaa tgattcggaa ctggtatagt
      601 cgtcattgtg taaaacaagg aactcatcca aagtaatatg taatttgtct aaaatattga
      661 ttagtcgaat acaagatatc tctgactccc cacgttcaaa acgagatatt tgtgattttg
      721 aaagatgttc atctgctaca gagcatagac taatttgttt tcctttacga acttttctaa
      781 gtgtgatgcc aagttttgat ttcataattt cccattttcc caacaaaaat taaatccgtt
      841 tttatttatt gtacaataaa taaaaacgga aaggaagtcc aagtatgaag aaaatttcaa
      901 aatttttacc gattttggtt ttggtgatgg atattatcat catttaaaaa agtgtattaa
      961 ttattgtatt tcacaattag ataatataac agtaacaggt ttagcacacg gttattctgg
     1021 agtagcaatg tcattatcat attattatga aattaatcct agtacagata tattaaaatg
     1081 tatagaggaa ttactatata aagaagataa atgtttcaat aatatattaa aaaattggaa
     1141 agacataaga ag
//
LOCUS       DAVKFH010000128         1081 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKFH010000128 DAVKFH010000000
VERSION     DAVKFH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.128"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            117..>1081
                     /gene="pavB"
                     /locus_tag="VW006_002105"
     CDS             117..>1081
                     /gene="pavB"
                     /locus_tag="VW006_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9380867.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLE"
BASE COUNT          369 a          198 c          224 g          290 t
ORIGIN      
        1 atataagtgt tttataagag ggatttaaga aaaattttaa ctttttctta gtccttttta
       61 atttcaggag attatactag agtcatcaaa taaagaaaga ctctaaggag aatcctatga
      121 aattcaatcc aaatcaaaga tatactcgtt ggtctattcg ccgtctcagt gtcggtgttg
      181 cctcagttgt tgtggctagt ggcttctttg tcctagttgg tcagccaagt tctgtacgtg
      241 ccgatgtggt caatccgacc ccaggtcaag tcttacctga agagacatcg ggaacgaaag
      301 agggtgactt atcagaaaaa ccaggagaca ccgttctcac tcaagcgaaa cctgagggcg
      361 ttactggaaa tacgaattca cttccgacac ctacagaaag aactgaagtg agcgaggaaa
      421 caaactcttc tagtctggat acactttttg aaaaagatga agaagctcaa gaaaatccag
      481 agctaacaga tgccttaaaa gaaactgtag atacagctga tgtggatggg acacaagcaa
      541 gtccagcaga aactactcct gaacaagtaa aaggtggagt gaaagaaaat acaaaagaca
      601 gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg gaaaggtcct ttcactgctg
      661 gtgtaaacca agtaattcct tatgaactat tcgctggtga tggtatgtta actcgtctat
      721 tactaaaagc ttcggataat gctccttggt ctgacaatgg tactgctaaa aatcctgctt
      781 tacctcctct tgaaggatta acaaaaggga aatacttcta tgaagtagac ttaaatggca
      841 atactgttgg taaacaaggt caagctttaa ttgatcaact tcgcgctaat ggtactcaaa
      901 cttataaagc tactgttaaa gtttacggaa ataaagacgg taaagctgac ttgactaatc
      961 tagttgctac taaaaatgta gacatcaaca tcaatggatt agttgctaaa gaaactgtcg
     1021 aaaaagctgt taaagataac gttaaagaca gtatcgatgt tccagcagcc tacctagaaa
     1081 a
//
LOCUS       DAVKFH010000129         1051 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKFH010000129 DAVKFH010000000
VERSION     DAVKFH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.129"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..481)
                     /locus_tag="VW006_002106"
     CDS             complement(<1..481)
                     /locus_tag="VW006_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9380868.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     "
     gene            complement(478..960)
                     /locus_tag="VW006_002107"
     CDS             complement(478..960)
                     /locus_tag="VW006_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU9380869.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL
                     "
BASE COUNT          401 a          184 c          119 g          347 t
ORIGIN      
        1 tcatggaaat taagcgttta gcaatcatat caatacttct tgcatccact gttgataaag
       61 tctcatcaat aaaaatataa tcccaatttt tagcaaaaat taaggataag cctaatttga
      121 attttgttcc agtagatgct gtcatatagt tatctccgat tttattatca aaaaaagtat
      181 taatttgttc tttatcctca aatgatagat ggatttgaaa agaatcacac aacaactgca
      241 aattttcttc tatagtaaat ggaacaaccc caagctgatt ggtatcaagg tatagtacat
      301 ctttggagac acaattcttt aaaccactat caaaagtctc tagcccagct aaaagttgta
      361 gaagggtgct ctttcctgaa ccattctctc cttcaactct gtaaattccc ttatcaatct
      421 ctagtgatat atttttcaat atacatttat cataactttt agattcattt tcaattttca
      481 taatttaatt ccctctatca acgctgctaa aattaataaa agataagaaa tgatatacca
      541 ttttaaatta ctttttatat aaataagtat atttttaatt cccctatcct tcaataatcg
      601 gtataaaaga tactgactca taccttgata ccaaataaaa ttcggtattt caataagagc
      661 atgaggtaat attcctttta ttgtagctac tattccaatc atctgaccag atatacccaa
      721 ttgtattcct aaagatagca aatcaaaaag ttgcaataag ggcgaaatcg gaaaacagaa
      781 tagatatagc aaaaaatttg tagaatttgc ctgtaaaact gtcaatgtaa attgtaattc
      841 cagtttcgga ggtatagtag caaaagtctc attttttaat ataaagctta aaagtaaacc
      901 tagtaaaaaa cagactatac aaaataacac atagtaaatc agaaaaccac tttttctcat
      961 ctaatgcccc ctactaaaat tacaaatatt atcagaaaac aatataaaac cagaaaactc
     1021 catctcaata aactaactgt acctttaatt a
//
LOCUS       DAVKFH010000130         1049 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKFH010000130 DAVKFH010000000
VERSION     DAVKFH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.130"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            56..967
                     /locus_tag="VW006_002108"
                     /pseudo
     CDS             56..967
                     /locus_tag="VW006_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          339 a          183 c          216 g          311 t
ORIGIN      
        1 taatttatag aaatatttta gcagctaagg tgtactgtta tagattcaat acactataga
       61 ctgtaatcaa acaacgattt ggcgaaatgt aaaaaaatat gaggagttcg aactcgactc
      121 tctccttcaa gaaacacgtg gtggccgtaa ccatgcatat atgacgattg aggaaaagaa
      181 agtctttctt gcccgccatt tgaaggctac agaggcagga gaatttgtta caattgatgc
      241 cttatttcag gcttataaaa aggagttagg tcgttcctac acacgtgatg ccttctatca
      301 actgttgaag caccatggtt ggcgaaatat tatgccacgt ccagaacatc ctaggaaagc
      361 agacgctcaa accattgtcg cgtctaaaaa taaaatctca attcaagaag aaaagaaagc
      421 gctttaaaag cagtagacgt tttcgtaagg ttcgcttgat gtaccaagat gaggctggtt
      481 tcggtagaat cagtaaactg ggatcttgtt gggctccaat aggagtaggt ccacatgtcc
      541 atagtcacta tatacgagaa tttcactatt gttatggagc tgttgatgcc catacaggcg
      601 aatcattttt cttaatagct ggtggatgta atactgagtg gatgaacgcc tttttagaag
      661 agctttcaca agcttatcca gatgattatc ttttactcgt tatggacaat gctatatggc
      721 ataaatcaag taccttaaag attccgacta atattggttt tacctttatt cctccataca
      781 caccagagat gaacccattg aacaagtgtg gaaagagatt cgtaaacgtg gatttaagaa
      841 taaggccttt cgaactttgg aagatgtcat gaatcaactt caagatgtca tacaaggact
      901 ggagaaggag gtgataaagt ccatcgttaa tcggagatgg actagaatgc tttttgaaag
      961 cagatgagta ttatatgcaa tttctttata taaaaagacc ggattgctcc gatctttcaa
     1021 tagttcatat tctcaatttc tattttaaa
//
LOCUS       DAVKFH010000131         1033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKFH010000131 DAVKFH010000000
VERSION     DAVKFH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.131"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            238..837
                     /locus_tag="VW006_002109"
     CDS             238..837
                     /locus_tag="VW006_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380870.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYTTIDKTSDATALLKLKLNNVDNQPATEVLSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNYP
                     VGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT          372 a          152 c          199 g          310 t
ORIGIN      
        1 ttaatatttg gagaagagga tggccatctt ctccattttt atgtgcagag ttatagtgac
       61 ttgatgctgg gaaagtacac tgtgactgct aatactcaat gaaaatcaaa gtgcaaacta
      121 ggaagctagc cgcaggctgt acttgagtac gtcaaggtga agctgacgtg gtttgaattt
      181 gattttcgaa gagtataaaa cattcctaga agttgctttg atttccctaa tctatttatg
      241 caaattttat gttattttac tataacagtt gttgctaagc caaataatag tggggaagtt
      301 catttagacg taagcattga agataatcag ggaggtagtg ggcataattt cagttctgtt
      361 tcaagtagct cacaaacagc taaatatgaa gaaactggtt ataataacaa tttatcatta
      421 tatacaacga ttgataaaac gtctgatgca acagctcttt tgaaattaaa gttgaataat
      481 gttgataatc aacctgctac tgaagttctt agttcaggaa ttactgtaaa attaaatgct
      541 aaagataatg ctggaaactg gacaagtgct tcgaataaaa aagaaataac agtaaaaatt
      601 gtttctgcta aaccgacata tccagacaaa atcttagtga aaaatcctga taatataaaa
      661 gatacagaaa aaaatgccat tattgaaaaa ttgaaagagg caaataaaaa ttatccagta
      721 ggagctccaa cctttgctaa aggtgaagga gagcatgcaa atgatattgt agcaacttat
      781 tcagatggta caacttatta tgtaccgtta aatgatgtga caaaatatgc gaggtagtgg
      841 ctgtaccact cacttattca cctccccgtg atttgtagta gtgataggct ttctaactat
      901 tattataaaa caaaataaag agcacaacac tttttcattc tgtgttgtgc cttgagtgaa
      961 acgaaaggaa tgaattataa atatgaaaag tatagtcact agcatagatg agcgcttgcg
     1021 tactcgctta cga
//
LOCUS       DAVKFH010000132          920 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKFH010000132 DAVKFH010000000
VERSION     DAVKFH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.132"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..654
                     /locus_tag="VW006_002110"
     CDS             <1..654
                     /locus_tag="VW006_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9380871.1"
                     /translation="LKGINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEV
                     DGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNV
                     VKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITT
                     SYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            678..>920
                     /locus_tag="VW006_002111"
     CDS             678..>920
                     /locus_tag="VW006_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9380872.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTT"
BASE COUNT          339 a          117 c          167 g          297 t
ORIGIN      
        1 ttgaaaggaa taaatgttac tattaaaaaa ggagaaactg ttgctattgt tggagaatca
       61 ggttcaggta agagtacatt agctaaaatt ttattaggtt tattagaacc taatattggt
      121 tcaatagaag ttgatggagt agaaaaagaa gaaattggtc aaacattgta tagaaagatt
      181 tttggagcag tgttacaaaa ttcaacccta agttatggta ccttaagaga gaatttgaca
      241 tttggacact ttgtttcaga tgaagaatta atgacaaatc taaattcaat tggtcttagc
      301 aatgtagtta aatctttacc tcttggatta gagacaatca tcgctgaaga aggtaataac
      361 ttttctggag ggcagcagca aatgatactt ttagctcgtt gtcttttgtc gaaaccttcg
      421 gtagttgttt tggacgaagc aacaagtagt ttagataatt tatctcaaca aattacaact
      481 tcttacttaa gtgaaatcgg taccactaag attttaattg cccatcgact agatactatc
      541 aagtctgcag ataagatctt agtaatgcat aatggtgaaa ttgtagagat tgggacccat
      601 agagaacttc ttgaactagg aggcatttat aagcaattgt attcaaataa ttagtttttg
      661 attaaaaggg taaatttatg aagattatga aaaaaaaata ttggacttta gcgatattat
      721 tcttttgttt gttcaataat tctgttactg ctcaagaaat acctaaaaat cttgatggca
      781 atataactca cactcagact agcgaaagtt tttctgaatc tgatgaaaaa caggttgact
      841 attctaataa aaatcaagaa gaagtagacc aaaataaatt tcgtattcaa atcgataaga
      901 cagaattatt tgtaacaaca
//
LOCUS       DAVKFH010000133          900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKFH010000133 DAVKFH010000000
VERSION     DAVKFH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.133"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            41..223
                     /locus_tag="VW006_002112"
     CDS             41..223
                     /locus_tag="VW006_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9380873.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            233..598
                     /locus_tag="VW006_002113"
     CDS             233..598
                     /locus_tag="VW006_002113"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9380874.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            595..>900
                     /locus_tag="VW006_002114"
     CDS             595..>900
                     /locus_tag="VW006_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9380875.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     "
BASE COUNT          364 a          113 c          150 g          273 t
ORIGIN      
        1 tgataactta aacatcaaag ttcaagagag agttcgtgaa atggcaaatg ataataagag
       61 ccattatttg atatacagag ttttaggtat ttcatttgaa gaaggagaaa atatcgattt
      121 gtatcaaaat aaaggtcgtt ttttatacaa atatgctggt tcatttttag aagaagctgc
      181 agtactatcc tttaacgaaa aatttggtac agaaaatact taaaaagtta acattcctaa
      241 ttctgaaaat acaaaaccta agacttttga aattgattgt ttagtcggag aaaaacacgc
      301 atacgaaata aaatggtggg atgcaactac agatggagac catataacta aagaacacac
      361 tagaataaaa gttattcata acaaaggata tataccaatt cggttaatgt tctactatcc
      421 aaatagaact caagctataa aaattcagca aactttagaa acattgtata acggtattgg
      481 agggaaatat tattatggag attctgcctg ggaacattta agagcagtga ccggtattga
      541 tttacttagt attctaacag atattgcaaa taaaaaaaca ggggtaaaat caaaatgaca
      601 gtattaaaag gagataactt agaaatatta aaaactattg aatcctcaag tattgattta
      661 atctatatgg accctccttt ctttacacag aaaacccaaa aattatctaa taacaaaaat
      721 attatgtatt cattcgaaga tacgtggact tcgattgagg attacaaaga atttttgtct
      781 gtaagattag aagaatgcaa aagagtgcta aaaaatagtg gcagtatttt cgttcattgt
      841 gataaaattg caaatcatca tattagatta attttagata atatctttgg agcagatatg
//
LOCUS       DAVKFH010000134          840 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKFH010000134 DAVKFH010000000
VERSION     DAVKFH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.134"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..491
                     /locus_tag="VW006_002115"
     CDS             <1..491
                     /locus_tag="VW006_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9380876.1"
                     /translation="LRDYSGELRFCGDDIKKTSYLNMVSNVLYVDQKAYLFEGTIRDN
                     ILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLARGLIR
                     NKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQFPKD
                     FI"
BASE COUNT          299 a           98 c          164 g          279 t
ORIGIN      
        1 ttttgagaga ttatagtgga gaattgcgat tctgcgggga tgatataaaa aaaacctcct
       61 atttaaatat ggtttcgaat gttctatatg tagatcaaaa agcttatttg tttgaaggta
      121 cgattagaga taatatttta ttggaagaaa attatactga tgaagaaata ctacagtctt
      181 tagagcaagt tggtttgagt gtaaaagatt ttcctaataa cattttagat tattatgttg
      241 gtgatgatgg gagattactg tcaggagggc agaaacaaaa aattacttta gctagagggc
      301 taattagaaa taagaaaata gtattaattg acgagggaac ttctgctatc gataggagaa
      361 cttcgttagc gattgaacgt aagatattag atagagagga tttgactgtc attattgtta
      421 cccatgctcc gcatccggaa cttaaacaat attttactaa gatatatcaa tttccaaagg
      481 attttattta agaatatagg ggagggaaac atgagattct attgtgaagc ttatgaggta
      541 attactgaag agaaaaaagt atcatttttt tatgtagatg agaaaccctt aaggtatctg
      601 ttatttgact atatatttct actagttcta gctaaaaaaa tatcccaatc ttatgaactg
      661 caccccaaaa gttagattta ttctgtctaa cttttggggt gcagttcatt ttgcatagtt
      721 ttggcattca tcctagatat ccatataaaa tgtctgagat tgttgaaatg tctaaaaaaa
      781 tatcagatct aaacgataaa aaagaatttc acaaaaagaa ctgtgagttg aggtttcaag
//
LOCUS       DAVKFH010000135          803 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKFH010000135 DAVKFH010000000
VERSION     DAVKFH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.135"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..643
                     /locus_tag="VW006_002116"
                     /pseudo
     CDS             1..643
                     /locus_tag="VW006_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          267 a          141 c          143 g          252 t
ORIGIN      
        1 cttactgaca atccagacgc ttatttgact gaaatagctt ctgaatttgg ctgtcatcta
       61 actaccatcc actatgcgct caaagctatg ggttacactc gaaaaaaaga accacaccta
      121 ctatgaacaa gacccagaaa aagtagcctt atttcttaag aattttaata gtttaaagca
      181 cctagcacct gtttagattg acgaaacagg attcgatact tatttttatc gagaatatgg
      241 tcgctcatta aaaggtcagt taataagagg caaagtatct ggaagaagat atcagagaat
      301 ttctttggtt gcaggtctaa caaatggtga gttaatcgct ccaatgactt acgaagagac
      361 gatgacgagc gacttttttg aagcttggtt tcagaagttt ctcttaccaa cattaaccac
      421 accatcggtt attattatgg ataatgcaag attccataga atgggtaagt tagaactctt
      481 gtgtgaagag tttgggtata actttttacc tcttcctccc tactcacctg agtacaatcc
      541 tattgagaaa acatgggctc atatcaaaaa gcacctcaaa aaggtattac caagttgcaa
      601 taccttttat gaggctcttt tgtcttgttc ttgtttcaat tgactatagc agattgaaat
      661 aagatgagaa caaatcgatt gggaaagtaa aattaatttc tataaatgtt ttagcaattg
      721 tttcgtacta tttcagattc agtctactat atatcataat ggacaaaggg caagctgtca
      781 agaaattaca gttattttag taa
//
LOCUS       DAVKFH010000136          799 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKFH010000136 DAVKFH010000000
VERSION     DAVKFH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.136"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..788
                     /locus_tag="VW006_002117"
                     /pseudo
     CDS             1..788
                     /locus_tag="VW006_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          286 a           70 c          172 g          271 t
ORIGIN      
        1 ccatggatgg gttgaatata acttaaaaaa gaaattcttg acttattttg atttttggac
       61 atattctatg ggagatgctt ttatttgtgt atctgaaact atgaaaaaaa gattggagag
      121 tataataaaa aaataagaag atagttgtaa taaataacgg aattaatgta tcaaatagcg
      181 atttagatgt agtaggtgtt caagacttta aaaaagaatt ttgtattcca aataacaaaa
      241 aaatcatttg ttatgttgga aggttggatc cagaaaaaag gccggataga ttccttgaat
      301 ttgcagaaaa attattttta gtcagagaag atgtgatttt tattatggct ggaaatggta
      361 gtatgtgggc tgctttgaaa gaaaaaattt gccatttgaa atgtagagat aatttcaggt
      421 tacttggtga aatctatcca gctactatag tttaccaaat atcggattta ttgtatattc
      481 cttctgatac agaaggaata cctatgtgtg ttttagagtc aatgtcacaa ggaactccgg
      541 ttttagctag taatgttggc gggttaagtg aaattattga acatagggtt gatggatttt
      601 tatttgagaa ggaagatgtt gagggagtgt gtgcttgtgc taatttttta ctcaatgatt
      661 ctgagtattt gaaatatata ggtgagaata gtaaatcaaa aataagaaaa catttttctg
      721 tgcaaaaaat gtttgtagaa accatgagag tatatgatga attattagag aagagtagtc
      781 atggatagga atatagatc
//
LOCUS       DAVKFH010000137          776 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKFH010000137 DAVKFH010000000
VERSION     DAVKFH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.137"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>776
                     /locus_tag="VW006_002118"
     CDS             <1..>776
                     /locus_tag="VW006_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9380877.1"
                     /translation="SCPECGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHC
                     SKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHF
                     KHDFSRLPEIMSWDEYAFTKGKMSFIAQDFEKLDIITVLEGRTQAIIRNHFLRYDRVV
                     RCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQLDRKS
                     HEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEIF"
BASE COUNT          235 a          158 c          136 g          247 t
ORIGIN      
        1 catcttgccc tgagtgcgga agtcaaatga agaaatatga ctttcaaaaa ccttctaaaa
       61 ttccttatct tgaaacgact ggtatgccta ctagaattct ccttagaaag cgtcgattca
      121 agtgctatca ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag aagaatcacc
      181 aaatccctcg tatcatcaac caaaagattg ctcaaaagtt aattgaaaag atttctatga
      241 ctgatattgc ccatcagctt tccatctcaa cttcaactgt tattcgtaag ctcaatgatt
      301 ttcactttaa acatgatttt tcgcgtcttc ctgagattat gtcttgggat gagtatgctt
      361 ttacaaaggg aaagatgagc ttcattgcgc aagattttga aaagctcgat atcatcactg
      421 ttcttgaggg tagaacacaa gctatcattc gaaatcactt tcttcgctac gatagagttg
      481 tccgatgtcg cgtcaaaatt attactatgg atatgtttag tccttactat gacttagcta
      541 gacaactttt cccgtgtgct aaaatcgtgt tggatcgctt tcacattgta caacatctta
      601 gccgtgctat gagtcgtgtg cgtgttcaaa tcatgaatca attggatcga aagtctcatg
      661 aatacaaggc tatcaagcgc tactggaagc tcatacaaca ggatagtcgt aaactcagcg
      721 ataaacattt ttatcgccct acttttcgta tgcatttaac caataaagag attttt
//
LOCUS       DAVKFH010000138          724 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKFH010000138 DAVKFH010000000
VERSION     DAVKFH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.138"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..652)
                     /locus_tag="VW006_002119"
     CDS             complement(<1..652)
                     /locus_tag="VW006_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9380878.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQIL"
BASE COUNT          249 a          140 c           88 g          247 t
ORIGIN      
        1 tcaaaatctg agatttatag cacaatattg atgatattct atcaatataa tgtttttcat
       61 caagttcctc ttgatacatt tttaattctt tagtttttcc catataacta accatactac
      121 tatcacttac atatgggaag tcctcataat atattacttt ataacgcata aattcaagcg
      181 cccttccaat actattcaca aaaacatgat caacatggtc accaagtgaa agcggacaat
      241 atacgacaca tttgtcgtct aaatgcatta acagctcttt tatgatatca ttctttaatg
      301 tgtcctcatt ttttaattca ctatagatat gacggtatag aaaattgcca tttctatctt
      361 tcctatagag acattcatag tacgataagt gtctaaaatc acactgtaga cgttcacaag
      421 ctaacctgtc ttctttcttc ctttcttcaa tcggatattt cccaaggtta cacaacttat
      481 gaaattgctt agcagagggc tgtagctgtt ggctcaaagg gtaaccagaa aatatagtaa
      541 taacaagtac aatttctcct tctgaagtta attttgaaat ataatcacca caggaaaaaa
      601 ttgcgtcatc taaatgtgga gataaaaaga tatacttagt attgttactc ataaccattc
      661 cctctacaat ttatctaaaa actcactaag tgtctgatta aattccacat catcaaaaaa
      721 attc
//
LOCUS       DAVKFH010000139          633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKFH010000139 DAVKFH010000000
VERSION     DAVKFH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.139"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>633
                     /locus_tag="VW006_002120"
     CDS             <1..>633
                     /locus_tag="VW006_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9380879.1"
                     /translation="DYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPFGFSNKL
                     KRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTANI
                     KNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRIGN
                     FYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQ"
BASE COUNT          227 a           78 c          113 g          215 t
ORIGIN      
        1 agattatgat gagaaaattg gtagttttaa tacatatgaa atcgaacaaa cggtatcgaa
       61 gattctgagg gtaaaaccta atggaaagat tattttaaag tcaacagttc cgttcggctt
      121 ttcaaacaaa ttaaaaaggc tgtttgatac aaaaaatatc atttttgtcc ctgaattttt
      181 gagagaaggt tgttctatat atgataattt atatccaagt cgcatagttg ttggagatga
      241 gacagttgaa ggaagaaaaa ttgcagagtt gttcctttcg attagtactc atagtactgc
      301 caatattaaa aatgttatgt tagtttctcc tactgaagca gaagcaatta agcttttttc
      361 taacacattc ttagctctcc gtgttgcttt ttttaatgaa ctagattctt ttgctgagag
      421 gagaagttta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa gaattggaaa
      481 tttttataat aatctttctt ttggatttgg aggatattgt cttcccaaag atactaaaca
      541 attaaaaaaa gaatttatag aaataaatgc cccagtgata gaagcgattg atatttcaaa
      601 cacaaataga aaacagttca tagttaaaca aat
//
LOCUS       DAVKFH010000140          633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKFH010000140 DAVKFH010000000
VERSION     DAVKFH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.140"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            192..410
                     /locus_tag="VW006_002121"
     CDS             192..410
                     /locus_tag="VW006_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9380880.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          209 a           77 c          118 g          229 t
ORIGIN      
        1 cggctagttt cctagtttgc tctttggttt tcattgagta taacacattg ttagaagttg
       61 gtttaaattt cctaatcagt ttgttcacat ttaccttcga tatattatat cccatagtta
      121 aggttggtca tacagatgat tatagccatg gagccgtaaa acttagtgtt tctttagttg
      181 acaaagatgc catgaaaaaa atatctgtaa ctgtaatagg atattttgaa ataaatatag
      241 atgaaaatat caccgatatt ctatacgtaa atggtactgc tattctttat ccttatttac
      301 gttctattgt ttcaatagtt tcggcaattg atagcagtga agcaatgttg ctacctacca
      361 ttaatgtttt agagttacta gataaatctc aaccttttga agaagaataa tttattagct
      421 cactaaattg agggtaagga aaagtaaaag cagtaagaaa aatgtcttgc attatacagc
      481 aactttttgg gaatgagtag atggattgaa taaaatttga ttaagagtgg atgatttatc
      541 tgtagattat tattggacag ttagtcttga agtagtctaa gaattaggtt ataatcagta
      601 gaagccttgc taataatgag gaggttagtt tat
//
LOCUS       DAVKFH010000141          629 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKFH010000141 DAVKFH010000000
VERSION     DAVKFH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.141"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>629
                     /gene="phtB"
                     /locus_tag="VW006_002122"
     CDS             <1..>629
                     /gene="phtB"
                     /locus_tag="VW006_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9380881.1"
                     /translation="LKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQV
                     AKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKE
                     KGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPH
                     YDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          200 a          129 c          139 g          161 t
ORIGIN      
        1 actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc
       61 tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga
      121 tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga
      181 tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag
      241 ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc
      301 taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa atactgaggc
      361 aaaaggagca gaagctatct acaaccgcgt gaaagcagct aagaaggtgc cacttgatcg
      421 tatgccttac aatcttcaat atactgtaga agtcaaaaac ggtagtttaa tcatacctca
      481 ttatgaccat taccataaca tcaaatttga gtggtttgac gaaggccttt atgaggcacc
      541 taaggggtat actcttgagg atcttttggc gactgtcaag tactatgtcg aacatccaaa
      601 cgaacgtccg cattcagata atggttttg
//
LOCUS       DAVKFH010000142          611 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKFH010000142 DAVKFH010000000
VERSION     DAVKFH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.142"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>611
                     /locus_tag="VW006_002123"
     CDS             <1..>611
                     /locus_tag="VW006_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9380882.1"
                     /translation="LQEQSLIYSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTK
                     YKKRSIVHILYNSATNVFQLFAPIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSE
                     ISIFNAYTQYILAAGYLNRVNDIWLENEENVENGLKKCSLEGRIDIKDLSFSYSKDSA
                     PVIENLNLTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGK"
BASE COUNT          232 a           74 c          105 g          200 t
ORIGIN      
        1 aattacaaga acaatcattg atttactcag tagaaacaga agcattaaat actatttctt
       61 caataaaaat atcgggatta gaagatgaaa tatatgaaaa ctggtctaag tatttaaaaa
      121 acgtattgac aaagtataaa aaacgaagta ttgtgcatat tttatacaat tcagctacta
      181 atgtatttca attgttcgct cctataatta tattgatttt tggacttgat aatgttttaa
      241 atggaaaaat tttattgggt gaggttgtcg catttcaaac catggcatca attttattta
      301 gttcagaaat ctcaatattt aatgcttata cgcagtatat tttggcagca ggatacttaa
      361 atagagtaaa tgatatctgg cttgaaaatg aagagaatgt tgaaaatggt cttaaaaagt
      421 gttctttaga aggtagaata gatattaagg acctatcttt ctcttattcc aaagactcag
      481 ctcctgtaat agaaaattta aatttgacta ttgagccagg acagagaatt gcactagtcg
      541 gtcaatcggg gtcaggaaaa agtacgttat caaaaatact atcaggatta tataagattg
      601 atacaggaaa a
//
LOCUS       DAVKFH010000143          599 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKFH010000143 DAVKFH010000000
VERSION     DAVKFH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.143"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(261..431)
                     /locus_tag="VW006_002124"
     CDS             complement(261..431)
                     /locus_tag="VW006_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380883.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(444..>599)
                     /locus_tag="VW006_002125"
     CDS             complement(444..>599)
                     /locus_tag="VW006_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU9380884.1"
                     /translation="CTQIIVAHRLSTIKDADVIFVMKGGKIVESGNHKYLMDLGGEYY
                     SLYTKRK"
BASE COUNT          201 a          119 c           74 g          205 t
ORIGIN      
        1 ctaggaagcg agccgcaggc tgtacttgag tacggtaaag caaagctgac gtggtttgaa
       61 gagattttca aagagtatta aacaaaaaga aaaaccgcta ctcctaagaa tagcgattta
      121 atcatgtttt taagctacta ctgtagttgc tctattattt aagtgttatg aataacttca
      181 atatctttaa agctttttgt tagatttata atttcaacta tagccatctc tactccttag
      241 tttacagcaa aaaattatgc ttaattctta ttgtttttgt aaacaatcag cgaacccaat
      301 actattccat tcaacaagcc taaagaaata ccatattcaa ctggaaaatc taaaaaaatt
      361 gcaaacacta ttccaatcat cacccctagt gaggctgttg ttaaaattac atattcattt
      421 tctttcttca ttttctttac acctcatttc ctttttgtat ataagctgta gtactctcca
      481 ccaagatcca ttaagtactt atgatttcct gactcaacaa tcttaccacc tttcattaca
      541 aaaataacat ccgcatcctt aatcgttgac aatctatgag ctacaattat ttgagtaca
//
LOCUS       DAVKFH010000144          587 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKFH010000144 DAVKFH010000000
VERSION     DAVKFH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.144"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(179..475)
                     /locus_tag="VW006_002126"
     CDS             complement(179..475)
                     /locus_tag="VW006_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9380885.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          212 a          107 c           71 g          197 t
ORIGIN      
        1 tcaaagtagc gtaatctact accaaaaaaa gtaaaaataa tttcccttga aaaggaagat
       61 agcttttaaa atagattgac aaacttatga taacaaaaaa caaaagggtt atcccaataa
      121 aattaagata acctaaaata ctttcacatt cttttttaga aagtattttc atctagttct
      181 accatagagc tgctttagca gcacctttct ttttcaatat ataaagaact gttgctgcaa
      241 tacccgaacc aatagaacct actcctgtga ttgcacctac aattgaaatt actgctgtaa
      301 ctgtactata agcactaatc aaattgacta cagtagcagc agcttttgtc gaaattccta
      361 atgtcccagc caacatagga aaagaaacaa cacctaagct taaccaacta atgactgcta
      421 acattgctga aacaataaga aatttattat gaaattccat tcttttagat ttcattttag
      481 tgtactcctt ttgtatatta tgtatgtttt attgagagtt aattcgcaca tgttgtattc
      541 ctaactctct tcaaatgttc gtctatattt tatactactt ttattaa
//
LOCUS       DAVKFH010000145          516 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKFH010000145 DAVKFH010000000
VERSION     DAVKFH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.145"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..268)
                     /locus_tag="VW006_002127"
     CDS             complement(<1..268)
                     /locus_tag="VW006_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380886.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLITFWDNQ"
     gene            complement(261..482)
                     /locus_tag="VW006_002128"
     CDS             complement(261..482)
                     /locus_tag="VW006_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9380887.1"
                     /translation="MKDEENLLIKESIDFYFDNVVSQVIKPELSTDLNTMNTFSFMLG
                     VSGVVYEISRKQDDRLLNVLLLELRGHDD"
BASE COUNT          172 a           94 c           61 g          189 t
ORIGIN      
        1 gttgattatc ccagaatgtt attaatggta acggaagttc actaatttta tttacatctt
       61 taacttgata tgagccagta tcaaaatttc gtttattcaa taactgtttc aagtcaccta
      121 tactataacc atctctccct ttttctatat cacgtcttaa atctagtaat gtctctttgc
      181 ttttatagaa atgtagtatg gacaagcagc aacataaacc acattctatt tgagacattt
      241 gctcaatatt aggtactttt ctagtcatca tgtcctctta actccagtaa taaaacatta
      301 agtaatctat catcttgttt tcgagatatt tcatagacaa ctccagatac tcctagcata
      361 aaagaaaatg tattcatagt attcaaatcc gtacttaatt ctggtttgat gacctgagaa
      421 acgacattat caaaataaaa atctatagat tcttttatta ataggttttc ttcatctttc
      481 aatattgcat caagacaact gatagtacct acagaa
//
LOCUS       DAVKFH010000146          506 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKFH010000146 DAVKFH010000000
VERSION     DAVKFH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.146"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>506
                     /locus_tag="VW006_002129"
     CDS             <1..>506
                     /locus_tag="VW006_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9380888.1"
                     /translation="LQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKIL
                     HYHKQKFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNF
                     KPAGKSIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKT
                     AESVNQAL"
BASE COUNT          183 a           92 c           99 g          132 t
ORIGIN      
        1 tttacaacaa gtgagaaaag ggctatacaa aaaggtctat tctgccgatt acgcacaaac
       61 tgtttaccaa ttcaatcgaa aacggtcggt gaaaaagtta attttaacaa aggaaatcag
      121 agagaagatc ttacactatc ataagcaaaa attttcgcct gaaatgatgg ttaacaagaa
      181 gcaagtgaaa gttggtattt caaccatcta ctactggttt cataatggtc atttaggatt
      241 gacgaaggcc gacatgcttt atcccagaaa aaggaaaggt gtcaagaagc aagctagtcc
      301 gaactttaag ccggcaggta aatctatcga agaacgtcct gacgttatta atcttcgctt
      361 ggaaaatggt cattatgaaa ttgataccgt cctactgact aagataaaaa attattgcct
      421 gttagtctta accgaccggc ggagcagaca ccaaattata aggttaattc caaataaaac
      481 tgctgaatct gtcaatcagg cgctta
//
LOCUS       DAVKFH010000147          443 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKFH010000147 DAVKFH010000000
VERSION     DAVKFH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.147"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            128..443
                     /locus_tag="VW006_002130"
                     /pseudo
     CDS             128..443
                     /locus_tag="VW006_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
BASE COUNT          187 a           51 c           72 g          133 t
ORIGIN      
        1 taaagaatca aaaactctta aacaaatatt tgaaactgct ttactcgagg ttaaagatga
       61 aatcggttta aactcatatg aattaacaga gtataataaa atgaaataat aataggacaa
      121 atcaatcagg acagtcaaat cgatttctaa caatgtttta gaagtagagg tgtactattc
      181 tagtttcaat atactatatt atcgcttacc aacatttgct tttatatttg ctaaactttc
      241 agaaaaaaag ttggagtata tgatgctagg tgactgcgtc atgttagtaa acgaaatgga
      301 aataacggat catagagtag ataacttatt tgaaaaaggg aaaaatgaga taaaagatcc
      361 aattggtaca aatagtgttt taaataaaaa aattatttta caaaaaatca gaaaattatc
      421 taatcaaccg agtgggtact gga
//
LOCUS       DAVKFH010000148          430 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKFH010000148 DAVKFH010000000
VERSION     DAVKFH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.148"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            17..235
                     /locus_tag="VW006_002131"
     CDS             17..235
                     /locus_tag="VW006_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9380889.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
BASE COUNT          177 a           49 c           85 g          119 t
ORIGIN      
        1 aaaaaagagg gatattatgg cacttaacta taaaccatta tggatacagt tagcaaaaaa
       61 aggactaaag aaaacagatg taatagctat ggcaggactt acaacaaatg ttatggcaca
      121 aatgggaaag gataaaccaa ttacatttaa gaatttagaa agaatatgta aggctttatc
      181 ttgcactcct aatgatatta ttagttttga agataatttt agtgacgagg aatagaaaat
      241 gactttaagg acagaagatc aagttaggga ttatgcaaga gaagtatagg ctttaatgaa
      301 gttgaagaaa acatcaatca aggtactggt caaataacta cttttaatca attaggcttc
      361 aagggatatt cagataagcc agatggttgg tatttaccta aaaatatgaa tgatgtagca
      421 ataatccttg
//
LOCUS       DAVKFH010000149          345 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060518
            SAMN33606286-rid17941373.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKFH010000149 DAVKFH010000000
VERSION     DAVKFH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606286
            Sequence Read Archive: SRR23727524
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722566.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:58:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,084
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 59 of 130
            Pseudo Genes (internal stop)      :: 45 of 130
            Pseudo Genes (multiple problems)  :: 30 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606286-rid17941373.denovo.149"
                     /strain="AUSMDU00060518"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            231..>345
                     /locus_tag="VW006_002132"
     CDS             231..>345
                     /locus_tag="VW006_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9380890.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLG"
BASE COUNT          118 a           53 c           78 g           96 t
ORIGIN      
        1 taccttagtc aagaattaga tgtttcacta tgtttgagta agttgatgat ttcatttgat
       61 aacaggtttg aaactgttag gctaggtggc caaggctaat catagccttg gtttagctga
      121 aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca
      181 cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag atgcaagaac
      241 attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt gagcggtgga
      301 agaatgaaaa taagtccaat cgtgaaattg caggcttgtt aggaa
//
